LOCUS       DATTQF010000001        62496 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.001, whole genome shotgun sequence.
ACCESSION   DATTQF010000001 DATTQF010000000
VERSION     DATTQF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 62496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.001"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(225..1070)
                     /locus_tag="VB241_000001"
     CDS             complement(225..1070)
                     /locus_tag="VB241_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP1243341.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNISSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQDDFVRIIKQLN
                     RKYGK"
     misc_binding    1299..1668
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1734..2837
                     /locus_tag="VB241_000002"
     CDS             1734..2837
                     /locus_tag="VB241_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1243342.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQKLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAEKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            2834..3994
                     /locus_tag="VB241_000003"
     CDS             2834..3994
                     /locus_tag="VB241_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEP1243343.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAMSE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTATHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTLER"
     gene            3963..5804
                     /locus_tag="VB241_000004"
     CDS             3963..5804
                     /locus_tag="VB241_000004"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEP1243344.1"
                     /translation="MSQFLTHLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLEKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKHTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            5801..8029
                     /gene="metE"
                     /locus_tag="VB241_000005"
     CDS             5801..8029
                     /gene="metE"
                     /locus_tag="VB241_000005"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEP1243345.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            8072..8824
                     /locus_tag="VB241_000006"
     CDS             8072..8824
                     /locus_tag="VB241_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEP1243346.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKCLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(8907..10088)
                     /locus_tag="VB241_000007"
     CDS             complement(8907..10088)
                     /locus_tag="VB241_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP1243347.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEINEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPAIMGIAPVDAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            10628..11761
                     /locus_tag="VB241_000008"
     CDS             10628..11761
                     /locus_tag="VB241_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEP1243348.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFATVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIASTGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(12130..12759)
                     /locus_tag="VB241_000009"
     CDS             complement(12130..12759)
                     /locus_tag="VB241_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEP1243349.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTGGWLVVIVYAVIGIVITSVSYISSIKIFNKQEL"
     gene            complement(12759..13601)
                     /locus_tag="VB241_000010"
     CDS             complement(12759..13601)
                     /locus_tag="VB241_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243350.1"
                     /translation="MTTLLNLDRVNKQYKSSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHAIDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(13626..14339)
                     /locus_tag="VB241_000011"
     CDS             complement(13626..14339)
                     /locus_tag="VB241_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP1243351.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATHNNVV
                     VISIIATIIIILIEIIVLMNQRRALKYKRLVDEEVDNEETDQYELLANRHVLNGSILS
                     ILQTVIALLVLLIFVVGQAEVNGILLFLIPFFASAIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGEHHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(14336..14539)
                     /locus_tag="VB241_000012"
     CDS             complement(14336..14539)
                     /locus_tag="VB241_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1243352.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNEKVETIFIIEEDEV"
     gene            14779..15168
                     /locus_tag="VB241_000013"
     CDS             14779..15168
                     /locus_tag="VB241_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEP1243353.1"
                     /translation="MAFKLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAPSSNQSIVQDVLTRHQQGQTDFETFCDEMAQAGIYK
                     WHIDIQAGTCTYIDLQEQAIISELIPQ"
     gene            15267..15491
                     /locus_tag="VB241_000014"
     CDS             15267..15491
                     /locus_tag="VB241_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEP1243354.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            15508..16209
                     /gene="tatC"
                     /locus_tag="VB241_000015"
     CDS             15508..16209
                     /gene="tatC"
                     /locus_tag="VB241_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEP1243355.1"
                     /translation="MVHFSELRQRLVKILLSFVIAVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFMGLAKFDLIDTYSLKHYRKYIYFACFVLASIIAPPDITLNILLTLPLILLFEFS
                     MFIVKFTSRNDLSSQ"
     gene            complement(16555..18267)
                     /locus_tag="VB241_000016"
     CDS             complement(16555..18267)
                     /locus_tag="VB241_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEP1243356.1"
                     /translation="MKHYLTKIIATIITLVMVFSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISKDNKLKAIEQVVSAVKKLSLEDNSEGNAVKSNVRKLEDAKANDDQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSGTSANDQYHVSQLNDELEKAIKAIDNNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIVLAITFVETLGNSGMLRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYTNAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFVIGIALAIVILIVFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(18248..19477)
                     /gene="efeB"
                     /locus_tag="VB241_000017"
     CDS             complement(18248..19477)
                     /gene="efeB"
                     /locus_tag="VB241_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEP1243357.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQSGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATQQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(19474..20328)
                     /locus_tag="VB241_000018"
     CDS             complement(19474..20328)
                     /locus_tag="VB241_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243358.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNKLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(20593..21138)
                     /locus_tag="VB241_000019"
     CDS             complement(20593..21138)
                     /locus_tag="VB241_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1243359.1"
                     /translation="MFGMKVNEQIRLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKSGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFDEIELNRIEISAAVNNEKSQAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYNEG"
     gene            21498..22493
                     /locus_tag="VB241_000020"
     CDS             21498..22493
                     /locus_tag="VB241_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP1243360.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFKGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLRGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(22553..23119)
                     /locus_tag="VB241_000021"
                     /pseudo
     CDS             complement(22553..23119)
                     /locus_tag="VB241_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(23133..24194)
                     /locus_tag="VB241_000022"
     CDS             complement(23133..24194)
                     /locus_tag="VB241_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP1243361.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGVFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAKTTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(24208..25134)
                     /locus_tag="VB241_000023"
     CDS             complement(24208..25134)
                     /locus_tag="VB241_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1243362.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMNDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            25446..26804
                     /gene="glpT"
                     /locus_tag="VB241_000024"
     CDS             25446..26804
                     /gene="glpT"
                     /locus_tag="VB241_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HEP1243363.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLVLT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYILIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(26888..27328)
                     /locus_tag="VB241_000025"
     CDS             complement(26888..27328)
                     /locus_tag="VB241_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEP1243364.1"
                     /translation="MYKSKILLKYIFSEESDVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKRAEQTQKWQCKYFFEY"
     gene            complement(27434..28789)
                     /gene="mepA"
                     /locus_tag="VB241_000026"
     CDS             complement(27434..28789)
                     /gene="mepA"
                     /locus_tag="VB241_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HEP1243365.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVALPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMSNKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGVVIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(28896..29315)
                     /gene="mepR"
                     /locus_tag="VB241_000027"
     CDS             complement(28896..29315)
                     /gene="mepR"
                     /locus_tag="VB241_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HEP1243366.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            29527..31482
                     /locus_tag="VB241_000028"
     CDS             29527..31482
                     /locus_tag="VB241_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP1243367.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTANTTILNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHIRIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAITVAVQPLINDGIVNSNYTAAILKQLEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLCRQDFLDGNIFKIKQHIALTMTKEV"
     gene            31487..31930
                     /locus_tag="VB241_000029"
     CDS             31487..31930
                     /locus_tag="VB241_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1243368.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            31932..32216
                     /locus_tag="VB241_000030"
     CDS             31932..32216
                     /locus_tag="VB241_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1243369.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            32231..33577
                     /locus_tag="VB241_000031"
     CDS             32231..33577
                     /locus_tag="VB241_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP1243370.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(33671..34696)
                     /locus_tag="VB241_000032"
     CDS             complement(33671..34696)
                     /locus_tag="VB241_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEP1243371.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAQHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQASGKRFIATADGQIDLLEIAKLIKEKRPEIAQKVAT
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(35012..36034)
                     /locus_tag="VB241_000033"
     CDS             complement(35012..36034)
                     /locus_tag="VB241_000033"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP1243372.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGARDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELSDADWESIEELASKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            complement(36012..36956)
                     /locus_tag="VB241_000034"
     CDS             complement(36012..36956)
                     /locus_tag="VB241_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEP1243373.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEDKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHKDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            complement(36946..37746)
                     /locus_tag="VB241_000035"
     CDS             complement(36946..37746)
                     /locus_tag="VB241_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEP1243374.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKLNQDLLAKCYLSCLKLADQQSLNHIAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(37747..38079)
                     /locus_tag="VB241_000036"
     CDS             complement(37747..38079)
                     /locus_tag="VB241_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEP1243375.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(38113..39114)
                     /locus_tag="VB241_000037"
     CDS             complement(38113..39114)
                     /locus_tag="VB241_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP1243376.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSETSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKYFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(39228..39326)
                     /locus_tag="VB241_000038"
     CDS             complement(39228..39326)
                     /locus_tag="VB241_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243377.1"
                     /translation="MESIFKIKIELMNVICRSENMNMKKKNTSEKI"
     gene            39422..40636
                     /locus_tag="VB241_000039"
     CDS             39422..40636
                     /locus_tag="VB241_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP1243378.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLSEQRPHDIDLEFSMADLENLAIPRAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDTSSKDNFS"
     gene            40694..41521
                     /locus_tag="VB241_000040"
     CDS             40694..41521
                     /locus_tag="VB241_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1243379.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIDDIFTSFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSGINTEPFKMANSYYAKIAQSVNETVLAQLTS
                     PTPIIQDQIAQRFLIYGYARGTGEWINMINIADYTDSKYNISPNELKTLPPVFIAHCN
                     GDYDVPVEESEHIHNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITTA"
     gene            complement(41763..43838)
                     /gene="lip2"
                     /locus_tag="VB241_000041"
     CDS             complement(41763..43838)
                     /gene="lip2"
                     /locus_tag="VB241_000041"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HEP1243380.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVTSHEAQASEKIPTT
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDMLQSSKHQST
                     QNSKIIRTQNDNQVKQDSERQGSKQSHQNNATNKTERQNDQIQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKEQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDNVHQSNQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     YKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLNGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     SKGTQLKAS"
     gene            44255..45568
                     /locus_tag="VB241_000042"
     CDS             44255..45568
                     /locus_tag="VB241_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEP1243381.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KFGPSILFKANYGPFLFEKLMMPLSILIPVSAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAIDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSVTFMIII
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            complement(45694..46362)
                     /locus_tag="VB241_000043"
     CDS             complement(45694..46362)
                     /locus_tag="VB241_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP1243382.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            46502..47302
                     /locus_tag="VB241_000044"
     CDS             46502..47302
                     /locus_tag="VB241_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1243383.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            complement(47579..48439)
                     /locus_tag="VB241_000045"
     CDS             complement(47579..48439)
                     /locus_tag="VB241_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP1243384.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYHLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            48596..49477
                     /locus_tag="VB241_000046"
     CDS             48596..49477
                     /locus_tag="VB241_000046"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEP1243385.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            49517..51049
                     /locus_tag="VB241_000047"
     CDS             49517..51049
                     /locus_tag="VB241_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEP1243386.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLLIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(51154..52374)
                     /locus_tag="VB241_000048"
     CDS             complement(51154..52374)
                     /locus_tag="VB241_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP1243387.1"
                     /translation="MNILFAITGIAFALFVAFLFSYDRKNIDFKKTLIMIFIQVLIVI
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPKL
                     SRAKLYTIATSGMSAVSMTMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(52385..53308)
                     /locus_tag="VB241_000049"
     CDS             complement(52385..53308)
                     /locus_tag="VB241_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEP1243388.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     VTTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     RIGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAVLGAKIAVAVNKLL"
     gene            complement(53283..54401)
                     /locus_tag="VB241_000050"
     CDS             complement(53283..54401)
                     /locus_tag="VB241_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1243389.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            complement(54744..55775)
                     /locus_tag="VB241_000051"
     CDS             complement(54744..55775)
                     /locus_tag="VB241_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP1243390.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            complement(55983..56660)
                     /locus_tag="VB241_000052"
     CDS             complement(55983..56660)
                     /locus_tag="VB241_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243391.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIVALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(56673..57722)
                     /locus_tag="VB241_000053"
     CDS             complement(56673..57722)
                     /locus_tag="VB241_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP1243392.1"
                     /translation="MFLAWNEIRRKKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            complement(57971..58861)
                     /locus_tag="VB241_000054"
     CDS             complement(57971..58861)
                     /locus_tag="VB241_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEP1243393.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQIVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEIK"
     gene            59445..60752
                     /gene="brnQ"
                     /locus_tag="VB241_000055"
     CDS             59445..60752
                     /gene="brnQ"
                     /locus_tag="VB241_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP1243394.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESVLSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALVFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            61003..61827
                     /locus_tag="VB241_000056"
     CDS             61003..61827
                     /locus_tag="VB241_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP1243395.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(62077..62475)
                     /locus_tag="VB241_000057"
     CDS             complement(62077..62475)
                     /locus_tag="VB241_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEP1243396.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
BASE COUNT        19643 a        11950 c         9575 g        21328 t
ORIGIN      
        1 acactttcat atgtttcaat tttagttaat ggttcgaata gcgatgaaat aatactcata
       61 acttgatcca taattagcgt tactcccttc tatatagtct caaataattt tattaatatt
      121 attgtatgct tccaacctac tgaagtcgca tcatcttcaa agaatataga taactatcga
      181 tatttctata gattcacaaa gtgatattat ataaaattgt gtaactattt accatactta
      241 cgatttaatt gtttaatgat tctcacaaag tcatcttgcg attcaaactc aaatgagatt
      301 ttgccgactg atttttttat cgatatatct acttttgcgc catactgttc tcgcaactgt
      361 cgttcttgtt gctttataaa tttaggctta gttatatcta ctttgtctgt ttccgacttt
      421 gacgaaatat tttttaattc attaacatgg ttttctaaat atctaacact ccacttttct
      481 ttaacaaccc gtttcgctaa cctaagcatt tgttgttcat ctttaattgc caataacgtt
      541 cgtccatgtg cacttgtcag tcgcccatct tttaccatgt cagcaatctt tttcggtaaa
      601 tgtaataacc tcaacatatt cgctatatac gggcgcgact tactcaatcg cttcgctact
      661 tcttgttgcg taattttcaa atctgtcatc aaacgttgat aactttcagc ttcttcaatc
      721 gcatttaagt cttctcgttg taaattttcg atgaccgcca gttccatcat atcttcatct
      781 gttaaatctt tgataatcgc tgatacgtat tttagaccag caattttcga agctctaaac
      841 cttctttcac caactacaat gtaataacct tgaactgttt ttctaagcac aattggttgc
      901 aaaataccat attgcttaat tgaatctgcc aaatcattta aatgattttc atcgaaagtt
      961 tttcggggct gatacggatt cggtttgata tccgatatat ctatttgctt aatttgttca
     1021 tcttttgttt tagtaatacg ttgatcttca ctttttgaca attcactcac atcacttttt
     1081 atttgataac aacagttaaa tatattttat gtattctaaa tgagtcagac aaccattaac
     1141 cacactttat ttaaaaaata attcattttt caacttcaag ataccaatta caatttcaca
     1201 aacaaattaa cttttaaatc aaaattttct gaataattgt tgacaaatct ttttttactc
     1261 tgtaagctaa ttaatatctt ataacagttt aatgaaacgt aaacacaata aagaggaaag
     1321 taaaacacac cctgcttata cagagagtct ttagtagctg agagaagatt ttgaaagcgt
     1381 gtttgaaaat ggccttggag tgttgatgcc aatatgaggt gtctacgggt tcgcccgtta
     1441 tagcgataca gtattaacat tgatgttaaa tggcgtactg gattctttac gcacgatttt
     1501 ttgttaataa gtatgggata gcacattact gtatccttac ttaacgactt aaattgtgat
     1561 aattgttcag taagcatatt tacttttaat gcgtactgaa taaggttatt tcagcgatgg
     1621 aataacaaat aaaggtggta ccgcgaaaca taagctttcg tcctttttat ccgattcatt
     1681 cgggtacgaa ggacggaagc ttttttattt tttcttacta ggagggtctt aatatgaagg
     1741 atacacagtt agcccaaatc acattaactg atgattcaac cggtgctata gcgaatccaa
     1801 tccatttatc tactgcctac aagcatccaa aactaggaca atcgacagga tttgattata
     1861 cacgtactaa aaatcctaca cgctcaacat ttgaaacctg ttttgccaaa cttgagcatg
     1921 gtattgcatc attcgctaca tcaagtggaa tgtcagccat tcaattaata tgtaatttat
     1981 ttaaaccaca cgatgaaatt ttagtttcat tcgatttata cggcggcaca tttagattat
     2041 ttgaatttta cgagcaacaa tacgatatca aatttaagta cgttgatttt acagattatg
     2101 aacaagttga aaaagaaatc actgataaaa cagtagcatt attcattgaa ccaatatcca
     2161 atccacaaat gattgctatt gatgtaaagc cgtactatca actttgtaaa gctaaaggct
     2221 tattgtcaat tatcgacaat acttttttaa caccttatct ttcaacacca ttagcagaag
     2281 gtgctgatat agtcttacat tcagctacga aatatattgg tggacataac gatgtactag
     2341 caggtgtcgt aaccgtcaaa gatgaatcac tcgcgcaaaa gttgtttgat tttcacaaca
     2401 tgactggcgc aacactatca ccaatagata gttatttgtt gttacgtgga cttaaaactt
     2461 tgcatttacg cattgagcgt gcgcaatcaa acgcaagaaa acttgctgaa aaatgtcagt
     2521 cacttcaagc aattgacgaa gtactatata gcgggcaaac tggcatgctt agtttaagac
     2581 ttaacaaggc ctatagcgtc gctaaattat tagaaaattt agacatttgc atttttgcag
     2641 aaagtttagg aggtactgaa acattagtga ccttccctta cacccaaaca catgttgata
     2701 tgccagatgc tgaaaaagat aaacgtggca ttgatgagta tttaatccgc ttatcactag
     2761 gtgttgaaaa ttatgaagac atcgaacgcg atatcatcca agcattagat aaagctcaga
     2821 ttggagagat tgtatgacac tttcaaaaga gacagaagtg atattcgatt ggcgtagagg
     2881 tgtggaatat cattcagcta acccaccact ctatgattct tcaacattcc atcaaacaag
     2941 tcttggtggc gatgttaaat atgattatgc acgaagtggc aaccctaacc gtgaactttt
     3001 agaagagaag ttagcacgat tagaacaggg gaaattcgct tttgcttttg catcaggtat
     3061 tgctgctatt tcagcagtac ttttgacttt caaatctggt gatcatgtca tcttacccga
     3121 tgatgtatat ggcggtactt ttcgcctcac tgagcaaatt ttgaatcgat ttaatattga
     3181 atttacaacc gtagatacta ccaagctcga acaaatcgag ggtgccattc aatcaaacac
     3241 aaaattaatt tatatcgaaa caccatcgaa tccctgtttt aaaattactg atatcaaagc
     3301 tgtttctaaa atagccgaaa agcatgaact actggtagct gtggacaata catttatgac
     3361 accgttaggt caatcacctt tattacttgg cgctgatatc gtcattcata gtgctacaaa
     3421 atttttaagt ggacatagcg atttaattgc tggtgctgtc attactaata atgaagcaat
     3481 gagtgaagca ctttatttaa tacaaaatgg tacgggcaat atgttatctg ctcaagatag
     3541 ctggacgctt gctaaacatt tgaaaacatt tccaatcaga tttaaacaat ctgtcgaaaa
     3601 cgcgcaaaaa atcgtgtcat ttttaataaa gcaagatgaa atttcagaag tttattatcc
     3661 gggacttact gctactcatt tagaacaagc taaaaatggc ggtgccgtta ttggctttcg
     3721 tttagctgat gagtctaaag cacaacaatt tgtcgatgca ctgacattac cactcgtttc
     3781 agtgagtctg ggcggtgttg aaacgatact ttcacatcca gcaacaatgt ctcacgctgc
     3841 actacctgaa gaagtgagac aagaacgtgg tatcactttc ggtctgttcc gattaagtgt
     3901 tggtctcgaa gatcctgatg aactcattgc agacatcaaa tacgcattaa aggaggcatt
     3961 caatgagtca attcctcaca cacttgaaag ataatgtttt agtagctgat ggcgctattg
     4021 gaactatttt atactctgaa ggtttagaca cctgcccaga agcatataac cttagccatc
     4081 cagataaagt tgaacgtatc catcgttcat atattgaagc cggtgctgat gtcattcaaa
     4141 ccaatactta tggtgcaaat tttgaaaagt taaaacgatt cggtcttgaa gataaagtta
     4201 aggctataca tcaagcagcc gttcgcatcg caaaaaaagc agcaaataaa gatacgtata
     4261 tattaggcac agttggtggg tttagaggta tcaaacaaga ggatatcagc ttacaaacta
     4321 ttctttatca tactgaaatt caaatagaca ccttgattga agaaggtgtt gacgcgctac
     4381 ttttcgaaac gtattacgac ctagaagagc taacaaatgt catttcacga acgagaaaga
     4441 aatacgacat tccaatcatt gctcaattaa ccgcttcaaa cacaaattac ttagttaatg
     4501 gtcaggcaat caatgaagga ttaaaacaac tcgttcaatg tggtgcaaac atcgtgggac
     4561 tcaattgtca tcatggccca caccatatgc aagagtcttt cacacatatt gaattaccag
     4621 agcacgcata cttatcttgt tatccaaatg ccagcttatt agatattgaa aatagtgaat
     4681 ttaagtatag tgacaatgca caatatttcg gtcaagttgc tcaaaatcta attcgcgaag
     4741 gtgttcgttt aattggtggt tgctgtggta caacgcctga gcacatcaaa tttattaaag
     4801 aatctattca gacacttaaa cctgttaatg acaaaaaagt gattccgata ccaacgaaag
     4861 cacttttcaa tccatctcaa aataaagtta gacaatcatt aacatctaag gttcaagaac
     4921 gtccaaccgt tattatcgaa ttggatacac cgaaacattt agacacggat agattttttg
     4981 aaaatatcgc taaacttgaa aaagctaatg tagatgcggt aacactcgca gataattcat
     5041 tggcaactgt cagaattagc aacattgctg ctgcaagctt aattaaacaa tattacaata
     5101 ttgaaccact cgtacatatt acatgtcgag accgaaactt aatcggcttg cagtctcatt
     5161 tacttggatt atcactcatt ggcgttaacg aaatattagc cataactggt gatccttcaa
     5221 aagttggtca cctacctggt gcaaccaatg tctatgatgt taattctaaa ggattaactg
     5281 aactcgctct aagatttaat caaggtatta acactgacgg tgatgcgctg aagaaacata
     5341 cacacttcaa catcgctggc gcctttaacc ctaatgttcg taaattagat ggtgccgtaa
     5401 aacgactaga gaaaaagata gaaagcggaa tgtcttattt tataacacaa cccgtgtaca
     5461 gcaaagagaa aatcattgaa atttaccatg ccactaagca cttgaacaaa ccatttttca
     5521 taggcattat gcctatcgca agttacaaaa acgcactctt tttgcataat gaagtgccag
     5581 gaatcaagat gtcagatgaa attttacaac aatttgaagc agttaaagat gataaagcca
     5641 aaactcgtga actaagcctt aagctttcaa aggatttaat cgatactgtt catgaatatt
     5701 ttaatggttt atacattatc acaccgtttc aaaatgtcga agattcatta gaacttgcag
     5761 catactcaaa atctattact gctcacaagg aggcaatatt atgacaacaa ttaaaacatc
     5821 aaacttagga ttcccaagat taggtagaaa aagagaatgg aaaaaagcca tcgaaagtta
     5881 ttgggcaaag aagatttcta aagaagaatt agatcaaaca ttaactgatc tacataaaga
     5941 aaatttatta ttacaaaagt actatcactt agatagcatc ccagttggtg atttctcttt
     6001 atatgaccac attttagata cgtcattatt attcaacatc atcccagaac gtttccaagg
     6061 aagaactatc gatgatgact tattatttga tatagctcgc ggtaataaag accatgttgc
     6121 aagtgcactt ataaaatggt ttaacactaa ctatcattac atcgtacctg aatgggacaa
     6181 tgttgagcct aaagtgagtc gcaatgtatt attagatcgc tttaaatacg cgcaatcttt
     6241 aaatgttaat gctcaccctg ttattgttgg tccgattact tttgttaaat tatcaaaagg
     6301 cggtcaccaa acttttgaag aaaaagttaa aacgttatta ccactttata aagaagtgtt
     6361 tgaatcatta attgatgcag gtgctgagta cattcaagtt gatgagccaa tcttagttac
     6421 agacgacagt gaaagctatg aaaatattac acgtgaagct tatgactatt tcgaaaaagc
     6481 tggtgttgct aaaaaattag tcattcaaac atacttcgaa cgtgcacatc ttaaattctt
     6541 aagttcttta cctgttggtg gtttaggatt agactttgtc catgataatg gctataacct
     6601 taaacaaatt gaagctggag attttgataa atcaaaaaca ttatatgctg gaattattga
     6661 tggtcgtaat gtatgggcaa gtgacattga agctaaaaaa gtcttaatcg ataaattgtt
     6721 agcacacact aatgaacttg tcattcaacc atcatcttca ttattacatg ttccagtatc
     6781 tttagatgat gaaacattag atacaagtgt tggcgaaggc ttaagctttg caactgaaaa
     6841 attagacgaa ttagatgcat tgcgtcgcct atttaatcaa aacgatagtg ttaaatatga
     6901 taaattaaaa gcacgttatg agcgtttcca aaatcaatca ttcaaaaact tagattatga
     6961 tttcgaaagc gtgcgtactt caagacaatc accattcgcg caacgtattg aacaacaaca
     7021 aaaacgtttg aacttaccgg atttaccaac aacaactatt ggatcattcc cacaaagccg
     7081 agaagttcga aaataccgtg cagattggaa aaacaaacgc attacagacg aagcatatga
     7141 aacattctta aaaaatgaaa ttgctcgatg gattaaaatt caagaagaca tcggcttaga
     7201 tgtattagtt cacggtgaat ttgaacgtaa tgacatggtt gaattcttcg gagaaaaatt
     7261 acaaggtttc ctagtaacta aattcggttg ggtgcaatca tatggttcac gtgccgtaaa
     7321 accaccaatc atttatggtg atgtaaaatg gacagcgcct ttaactgttg atgaaacagt
     7381 ttatgcacaa agcttaacag ataaaccagt taaaggtatg ttaactggac ctgtaacaat
     7441 tctaaactgg tcatttgaac gtgttgattt accacgtaaa gttgttcaag atcaaattgc
     7501 tttagcaatc aacgaagaag tattagcact tgaagctgca ggaatcaaag ttatccaagt
     7561 tgacgaacct gcattacgtg aaggcttacc attacgctct gaatatcacg aacaatatct
     7621 taaagatgct gttttatcat ttaaacttgc aacgtcttca gttcgtgatg aaactcaaat
     7681 ccatacacat atgtgttatt ctcaattcgg tcaaatcatt catgctattc atgacttaga
     7741 tgctgatgtt atttcaattg aaacatctcg tagccatggt gatttaatta aagactttga
     7801 agatattaat tatgatttag gtattggtct aggtgtatat gatattcata gtccacgtat
     7861 tccaacaaaa gaagaaatta ctacagcaat caatcgttca ctacaacaaa tcgaccgctc
     7921 attattctgg gtaaaccctg actgtggttt gaaaacgcga aaagaagaag aagttaaaga
     7981 tgcattgact gtgcttgtga atgctgttaa agctaaacgc caagaataat cgcagcattt
     8041 atcatataat aaactaaaaa cgggtgattt tatgacacaa tatcctttat ggcaacaatt
     8101 aaatacttta aaacaagcaa cttgggttga tttaacgcat acgtttgatc cggaaatacc
     8161 acgttttagt gagtttgaaa aaggcgaagt ttcaacttta ttcacggtta aagatcatgg
     8221 tttctatgtg caacgttgga atattgtaac gcaatatggt actcacattg atgcaccaat
     8281 tcactttgtc gagaataaac gttatttaga agacattgat ttaaaagaat tggtattgcc
     8341 gttaattgta ttagactttt caacagaagt agcgaacaac aatgatttca tcgtaacacg
     8401 tgcgcatata gaagcatggg aaaaagagca tggtactatt gaacctggca cttttgttgc
     8461 acttcgtacc gattggtcaa aacgttggcc taatattgaa acgtttgaaa acaaagatgc
     8521 gaacggacaa caacatgcac cgggttgggg attagatgct ttgaagtgct taattgaaga
     8581 acgccgtgtt gaagctgttg gtcatgaaac attcgatacc gatgcatctg tggacgttgt
     8641 aaaaaatggt gatttagttg gcgaacgtta tattcttggt caggataaat accaagtcga
     8701 gttattaact aatttagatc aattaccaac tcgtggcgcc atcatttacg ctatcagccc
     8761 taaaccaaaa gatgcacctg ggttccctgt tagagcattt gcaattaaac catcaaatga
     8821 ctaatattac ccaataaatc cataaacaaa taaaaacagg ttatgccaac atgtaattaa
     8881 tactgttgtg ataacctgtt ttcttattat ttatactttg atacaactgc agcgatagct
     8941 tgaccgccac caatacacaa tgatgttaaa ccagtttcaa cttcatcatt cagttgatgc
     9001 aataatgtca ctaaaactct agcaccagat gcaccaatag gatgtcctaa tgcaatagcg
     9061 ccacctttaa cattcacctt ttcaggaggt aattttaatt cacgatcaac agctaatgat
     9121 tgtgctgcaa atgcttcatt taattcaaat acatcaatat cacttaattc ttttttacta
     9181 cgtttcaaag ccttttcaac agcgtcaact ggggcaatac ccataatagc aggatctaca
     9241 ccatgacttc caaagccatc aagcactgcc aatggttcga tatttaattc tttagctttg
     9301 tcttctgaca tgactaacat catcgcagca ccatcattga ttcctgatgc attacctgct
     9361 gtaactgtac cgtctttttt gaaagctggt cttaagcgac ttaatttttc aactgataca
     9421 ttttcacgta caccttcatc cttagtgaca acgattggtt cacctttacg ttgaggaatc
     9481 gatactggaa ctatttcact atcaaattca ccattttgct gtgcacgtac tgctttttgt
     9541 tgtgagttta cagcaaatgt atcttgttct tctcttgaaa taccatattg ctctactaaa
     9601 ttttcagcag taatacccat atgatattga ttaaatacat ctgttaaacc atcatatacc
     9661 atgctatcaa ccattgattg atgtcccatt ttaaaaccaa agcgactgtt gttgacaagc
     9721 attggtgatt gagacatatt ctccataccg ccagctagca cgatgtcatt ttcaccagtc
     9781 acaatagatt gatatgctaa ttgaatcgac tttaacccag aaccacatac tttattcact
     9841 gtaaatgcag gtactgtttc tggcaagcca cctttcatag cagcaattcg tgctggattt
     9901 tgtccttgtc ctgcttgtag tacgttaccg atgatgactt cattaatctc acttggattc
     9961 aaacccgtct ctttaataat atgttctatt aaagtcgcac ctaaatcata ggctggcacg
    10021 tctttaaacg cacctccaaa aacgccaata ggtgtcctgt atgctgctgc taatacgact
    10081 ctcgtcatgt catattcatc tccttatgat atatacaatt ttattactaa tacgtctaat
    10141 gaaccaatac gccactaaca tccaatttat gaaaccaatt gctacgtatg ttacattttc
    10201 attcttgtta tgcatttaac aaatcatatt atacaatgat gtaatttgtt tgggcaatcg
    10261 gaaacgtaaa cggtttcact ttaacatttc acactcttaa atatgctcta tcatcctcat
    10321 ctatagtaca aaattatgct ttgatttacc acaaattatc attaatcatt tcgcgtaatg
    10381 actctaatta aatgctatct atcattgcat cgctattcac agcttgtttc accatcaatt
    10441 tcaccatcta ctttttcaaa ccatgacgtc tctattcaaa tattaaatcg ctgataactg
    10501 ttaatgccgc aaccagctcc ctgtcacccc tccttccatt tcatcgtcaa atctgcccct
    10561 attcactaga aaaaataaat agtatactta gtaaattaat tacacacctt aaaggagtta
    10621 aaattttatg gttaatgcat taacaccaaa acatttagct gaaaagcgtg caggattcca
    10681 tgagttgttc tttgatttaa ttttcgtcta tgccattcaa aaaattgcac atgtgatttt
    10741 aacaacacaa aatggttcca tttcagctga tttatttttc aaatacatcg tcatgagttt
    10801 gttcttatgg ttaatgtggt ctcatcaaac attttttaca aatcgatttg gtcaagttac
    10861 attcaaagac gtctcgttta tgatgtttaa tatgtttatc atggtcttcc tatctaacag
    10921 cctatatccg gactttgaaa agacattctt cccattcttc ttatgcgtgg caattatgta
    10981 tttaagtatc ggattacagt atttattaca tatacgtaca ggattagatt atggcgataa
    11041 gcgtacatgt caagcttttg cgaccgtagc ctttgtgatt tcatttttat catttctatc
    11101 attagtacta ccacaaagta tccattacat ccctggattt ttaggcgtat tcattgcatc
    11161 gacaggactt attccatttc aaaaatatct cgtattatca cctgttaata tgatgcattt
    11221 agtagaacgt ttttcattac tgacaattat catctttggc gaggtactgg ttggtttagc
    11281 ttcgtcatca tttagcattg atcattttag ttacatttat atattccaat ttatgatttt
    11341 aattagtctt ttcggtgtat attggatcat tactgaaaat tacattaatc ataaattgtc
    11401 atctatcggt ttccgtttat cgtatacgca tttattaatc aatatcgctt taggtgtgat
    11461 taatgccgca attgttttta gtaataacaa taaattaaat gacttgtttg aaattaatat
    11521 gatgtatatc agtgttttaa tattctatat tggattatgg ttaatcacgc cttatttcca
    11581 taacgaattg acaaatgcga agtatatttc atattcacta gggatactcg ttatgtctta
    11641 tattgtcagc cttttattta aaggtcacga ccatgtaatg attatctcag tatcagttgc
    11701 aaccttctgt atcatgctga tttattttaa aaatcaacgt ctacgtaata aacatgaata
    11761 aatagtattt acctcggtca aagtatttga actgaatcca aaagtgtcga acttcacatt
    11821 atactttgtg acaaacttca gttatgatac atgatcgagg tttttaaata tttccgagga
    11881 aatcgttagc ctatacaatc atcaaatgat actcataaat aacatatatt ctctaacccc
    11941 cactatctaa aaggaaataa ttccctcaca acataaccac ctcaaaacaa ataaatcaat
    12001 ctagatatgt cacctatact tatcaccccg catttcattc cacaccaaaa agaacagatg
    12061 acaatcgtaa agatttatca cctgctctaa atattatttc aatgactagc ttcacaccaa
    12121 gaatattgat tatagttctt gtttgttaaa aattttaata gacgatatat aagaaacact
    12181 tgttataact ataccgataa ccgcatatac tatgacaact agccatcctc cagtgctaat
    12241 tttttcaaat tctaatgcag atgcaccact aaccatacca tatataaaga aaaagaccac
    12301 gaaagaaata agtagtagta tagatgcaca tatcataatg acattggagt tttcagcgcc
    12361 aaatttaaat gtcaatggga aaatgataga gtatgcccct atgataccaa aactcattaa
    12421 acctgacata ataccaatca tcacactttg ggtcacaatc gtagtcacaa ctaatccaat
    12481 cattaaactt gcaccgaata agattaaata aaaggcaaag tatgacttaa tataatcact
    12541 acgtttaacc ggtaaagtag atacataata catccatctt gagtcttttt catgtttaat
    12601 attatcagta ataggtgtaa ttaacataac cccagccatt gccgaactca tcaacggatt
    12661 aaatactgca aagtatcccg cagctatgat agcgactata aaataaatat atgtttgctt
    12721 tctcgttgca taaaaactac ttaggaacat acctttcatt atacttcacc tcgcattatg
    12781 attttcgtag cgtcatcaat gtgttttaaa ggttctgcat tttcaatttc tgcataatct
    12841 ttaactaaaa tttgatattt ccccttcgat aatctagaag caatgattaa atgcttagga
    12901 atttcaacat ctttatcttc tgtcgtaaca attccatatt gtgctaaaag tatatctttc
    12961 tgttcagtta aaatcattcg tccatctttc ataaacacta atttatccgc taattgttct
    13021 atatcttcag aaatatgaga tgatattaag atgcctccac cttgcgcgac aaaatcttct
    13081 aatagttcca ttacttcttc tcgtccagaa acatccatac ccgcagttgc ttcatctaag
    13141 atcaatagct tcacatcatg agaaagcgca atcgtcaaag ctatcttcat tcgcatccct
    13201 cttgaaaaag ttttaatttt agtttgtagt ggcaactcga aatatttgat taaatcaaag
    13261 aatttttgac tattccaagt catataaata gattgaaata ctttatcaat atctttaata
    13321 gtcaatttat taggtactct caaatcatca aacactacac ctatgtgttc tttatattcg
    13381 acatcatcaa tagcatgttc ttccccaaaa aatgtgatac taccgttatc tttatgtcga
    13441 ttgcctacta gaatattaat taatgtcgat ttacctgagc catttttccc aattaatcca
    13501 actgtctcat tggtagaaat cgttaaagat gcatcttgta atttaaaatc cgaagacttg
    13561 tattgtttgt tcacacgatc taagtttaac aatgttgtca tatccatgct cctctttgtt
    13621 taattttaat aaaaacgtct tctcttcaat aaataactaa aggcgttata tatgaaaata
    13681 gcaatgataa gtagcaaact aaagctttga ttaattcctg tagcaattga ataagaccct
    13741 ataaaaataa tggctagtat caatatggat aagttgattg catatgtttt aaataatgca
    13801 attaattcta tatggtgttc accttcatcc aatatttcca atcgcttttc agtgtaattc
    13861 ttatctgcaa tttttggcat tctatcatca aatcttctat taaacagcgt aaattgtgta
    13921 ttgaaaatag cactagcaaa aaatggtatt aaaaataata gtattccatt tacttcagct
    13981 tgtcctacca caaaaataag caacactaac aaggcaataa ctgtctgtag aatacttaat
    14041 atacttccat ttaaaacatg acgattcgca agcaattcat attgatctgt ttcttcgtta
    14101 tctacctctt catctacaag tcgcttatac ttcaatgcac gtctttgatt catcaaaaca
    14161 atgatttcta ttaatatgat gattatagtc gcaataattg atattacaac gacattgtta
    14221 tgcgttgcga acgtcaaatt ctcaataccc aatccatcaa agttacctaa aattccacct
    14281 atgataccac ctacaagtcc acctagtaaa agatatccga tatatcttag tattttcaaa
    14341 cctcatcctc ctcaataata aaaatagttt ccaccttttc attgaagata cgagcaatct
    14401 ttattgcagt taataccgat ggcataaagt tgtttcgctc aattagcgaa atagtttgtc
    14461 ttgaaacacc cgcttgttta gcaagctgcg tttggtttaa gccatctcgt gctcgtaatt
    14521 ctttcaatcg attacgcaca tcgcatcaac tcctcaacta cactgtaaat gataattgtc
    14581 tttttgacaa ctatatttgt caaatctaca caaaaaatat aagtgtccac ctatgtatga
    14641 cattttgaaa cacacacctc aacacctaca agtcataatt gtttactttc gttacacctt
    14701 cctgcataat taacagcatt ctaattttag tatgatgcac gcattttcac taaatcaaac
    14761 cattcaaagg agattattat ggcatttaaa ttatctgcaa ttcaacaagc acatcaacaa
    14821 tttactggtg ttgattttcc aaaactattc aaggctttta aagatatggg gatgacttac
    14881 aatatcgtca atattcaaga tggcacagca acatacgtac atcaatcaga agatgatatc
    14941 gttacgtcat ctgtaaaaag taatcatcct gtcgctccaa gttcaaatca atctatcgtt
    15001 caagatgtat taactagaca tcaacaagga caaacagatt tcgaaacatt ttgtgatgaa
    15061 atggctcaag ctggcattta taaatggcat atcgatattc aagctggtac ttgtacttat
    15121 atcgacttgc aagaacaagc tattatttca gaattaatac ctcagtaagt tgtgtttata
    15181 gcaatatttt aattattata taaaatttgt tgataatcat tatcgttcgg tataaagtaa
    15241 atactatata ctacttatga gtgaggttga ttatcatgat aactaacact tttattttag
    15301 gcatcacagg tccaacaagt cttgtcgtca ttagcattat cgctttaatt atttttggtc
    15361 cgaaaaaatt accacaattt ggtcgtgcta tcggttctac tttaaaagaa tttaaatctg
    15421 ctacagaaga tttagataaa gagtctcacg atacacccag taaggaatcg aagcaacagc
    15481 gagagcaata gcactgatga tacattaatg gttcacttta gcgaactacg ccaacggtta
    15541 gtaaaaattt tattgtcgtt cgtcattgcg gtcatcgtcg tatatgtttc atctttttgg
    15601 tggatgacac cattcataac gtatattacc cgtgcacatg tgtccttaca tgcattttca
    15661 ttcacagaaa tgatccaaat atacgtgatg attatatttt tcatcgcatt ttgtttcatt
    15721 tcaccagtca tgttttatca attatgggca ttcattgcac ctggattaca taataatgaa
    15781 cgtcaattta tttataaata tagttttttc agcgtgttat tattctgtgc tggcgtcgct
    15841 tttgcatttt atgttggctt tccaatgatc attcaattcg cgttaaaatt atcattaact
    15901 ttaaacattt cgcctgttat tggctttaaa gcatatttga ttgaactcat acgttggtta
    15961 tttacatttg gcattctatt tcaattacca atattattta tgggtcttgc aaaattcgat
    16021 cttatagata catattcttt gaaacattat cgtaaatata tttattttgc ttgctttgtg
    16081 ctggcgagta tcattgcacc accagacata acattaaata tattactcac attgccactc
    16141 atattgctct ttgagttcag tatgttcatc gttaaattca ccagtcgaaa tgacttatcg
    16201 tcgcaatgac cttatacgaa tcaatactgc aatactcaca atgcaacaac atcacaatag
    16261 ccaataaaag aacaatgtag cacctcagat tatatcaata ctgcagctaa ctaacattgc
    16321 gttaatcaga atacagcatc ggattacacc gtgatatccg caatgcaacg agaaccaaca
    16381 atgcttcgat atctgcaata caacgagaac caacaatgca gtgatatccg cacctcaaca
    16441 atgccgcgat atccacaatg ctaagataat ccgcacctca acaatgcggc gatatctgca
    16501 atgcttcgat atccacaata cagcaacaaa aaaaacaggc actcggcctg ttttttattt
    16561 tttaaattta aagattaaga tagcaactac cataatatag gcagcttgtg ctatcaatgg
    16621 ttcgtaactt ggatagaatc ctaaccagtt aatcgttggg aatccttcaa taacatgtct
    16681 tggcatcgca cctaataatt gtaacttttg aatacttacg ccaagcattt tgaatcccat
    16741 aataaaaata aagatcgaca atactctaaa tatatagaat ataggtatca atctgacgat
    16801 aaatctaaat aatagcgcaa agacaattaa tatcacgatg gccaaggcaa tacctatgac
    16861 aaaatctttg gtcgctaatt cgcctatcat ccccatatag aaaatgataa cctcaacacc
    16921 ttcacgcaac acagatatta aaccaatcgt cgctaacaat accaaattac cattactaat
    16981 cgcattggta tacatatttt taatcatatc attccagcgt ttcgcattcg aacgtttatg
    17041 catccaaaca ccaacgataa acattaatat gaccgcaacg atacctaatc ccgcttccat
    17101 actctcacga agcataccgc tattccctag tgtttctacg aatgtaattg ctaaaacgat
    17161 actcaataca agtccggcaa ttgcaccacc aatcacactt gcagtaccct tcttatcttt
    17221 gacattacgc gtcatagtag tcaatgtcat tacaattaat aacacttcta gtccttcacg
    17281 taaaaagata atcatcacat cgacgaagct ataactatgg ccaacaacct ctttaatttg
    17341 attgtttata tctactaaac catctttgac atgtgcttta ttatgttcgt ctaatacact
    17401 ttgataatat ggtattttat cttcaatttt cgtatacaaa gcaccgtctt tagtttgaat
    17461 ttgaccttca acatatggcc aaatttctat aaaatgtgta agcgcagcat cagcatccga
    17521 caattgattg ttgtcgatag ctttaatcgc cttctctaac tcatcattta attgtgatac
    17581 atggtattga tcatttgcag acgtaccact tttcttatcg acatgatcaa tatttgattt
    17641 aaaagttgtc caagcatgtg acacttttgc cgtatctaat ggtgacttat gaattgcaat
    17701 tctaagttgt aataatgcga cttcaatttg tccatattga tttgcgtcat aattgcgaat
    17761 cactgtttca ttactagtcc aaatctgatt taaactattg ttcaaagatt ctaattccgc
    17821 tttatttttg tctttaatcg cttttgtcat cgctgcatct ttggcatcga cttgctgttg
    17881 caacagttta attttagaac ccgcatcttt actagccaat ttctcttcat aagcaattaa
    17941 tgacttcgtt aattgcgaaa gtgtatcttt ttgatcatca ttcgcttttg catcttcaag
    18001 ctttctcaca tttgatttga cagcattacc ttcgctatta tcttcaagcg ataatttctt
    18061 aactgcactt accacttgct caattgcttt cagcttattg tctttcgata tcgaattatt
    18121 agaaagtgca gattttgcat ccgttatcac actatataca tcactaatac tttgttgttc
    18181 tgctgcctga cttttcaata acccaaagct aaataccata actaaagtaa ttatcgtcgc
    18241 tataatttta gtcaaataat gtttcaccaa ggtatcctcc cttactaaca cctggtaata
    18301 ctaaaaatga agcagaacct ctatgtgtaa tgtattcatt taacttatca ttactaccta
    18361 aattgttttg aatatcgata aattgttggg tcgctttttg aaaagcaatg aacaacaagc
    18421 ctgtttcgaa gttacctgtg cggtcatccg taccatcaac ataattaaag gctctacgta
    18481 aaattgacgt atttgcttct ttcgctagtc tcgtatgggc atctttatca ataatatact
    18541 cgccatgact atctttcgct tttaagtcaa tttcatcaaa ctctttccca cctgttaacg
    18601 gtgcaccact atgtcgcttg cgaccaaatg tagcctcttg ttcttccagt gcagtacgat
    18661 cccacgtttc aatgtgtatt tgaatacgtc tgacaacaca ataagttcca tgtttcgccc
    18721 atccgtcatc aataaacaca taatctttaa gttgattact ctttctagga ttaattgttc
    18781 catctttaaa tgccattaaa tttctaggtg tttccttacc tttagcagag ataaaaccag
    18841 attgcgccca acgtacctta acaatatctc gaaatggacg aactaaatta tgaaccgcat
    18901 gaaaggatac ttgcgaatca tttgagcatg cttgaatcat aatatcacca tcgctgtaat
    18961 cgtctattaa ctgatcattc ggaaaatgcg gtaaatcttt aaaggcatcg ggaattttac
    19021 tagataaccc aattttcttc atcaaagact tactaattcc aaaggtaatc gttaacttgc
    19081 ttgcacctaa tccaatcgat tcaccagtat ctactggcgg cattaaagga ttgttactcg
    19141 gcttgccaac tgtatcacca tccatcatac gatcagccat aaccgtccac tttttaaaca
    19201 ttgccttaat tgcatctcta tcttttgact tcaaatctaa tgcaacgaaa ttgcatgttt
    19261 tttgcgtggg tgtagtaatg cctgattgca ctttaccata aaattcatac gcatcttttt
    19321 ccggatcttc tggtgtattg aacattgatt tgaaagacca catgctacca acaccacttg
    19381 cgccaattgc aacaccggca ccgccaatac ctaacatttt caaaaatgta cgtcttgaaa
    19441 attgcgtact atcgttaact tgttcataat ttgtcattca gtaatcacag ccattttact
    19501 tagtggctct cctaatgcat taacagcatc cgcaaatgct ttacggtctt tcttcgatac
    19561 tttttcaaat gactcataac cgccgttgtt atctttatac ttatccaata atttattcac
    19621 tttatcgaag ttcatttgaa tatcatcact taatttttta tcttttttct ctaaaatagg
    19681 tttaaataag tcataaattt tttgtgcgcc ttcaacgttc gctttaaaat catataaatc
    19741 tgtatgtgaa taaatttctt cttcacctgt gattttagaa gttgcaactt catttaatag
    19801 gtcaacagaa ccttgtaaca ttaattttgg tgtgatatct aatgtatcag ctttggcatg
    19861 caattctttt gcatctttca ataattgttg tgcgtctttt ttagtcacat catcaatttt
    19921 cttatcttcg tataatgctt tttcaatctt atgatatcct gaccattctt tttccttttt
    19981 ctcttctttc atatctgcaa gacgtgcatc aattttagga tctaaatctc caaatgcttc
    20041 tgcaactggt tcagagcgtt cataatacat acgaactttt ggatataacg cttttgcttg
    20101 ggccatatca ttattttcaa tcgctttaac aaatttttct gtacctttta aaaattcatt
    20161 taactgttca tcagtatatt tatcatatgc ttttgttgct tgttttaatt ctgctttaac
    20221 accatcatct tttttcgatg tctttgaatc atccttctta ctatcgtcgt tcccacatgc
    20281 agcaattaat aacgttgatg ctaataatag cgttgttaac tttttcattt tgacacctca
    20341 ttatagaaat tctattatcc ccatagacat gctctcattc gtactatctc acactcccta
    20401 aatgccaaga taatacgtgg ctcttaaaat gatgatttta aggcatcctc ccatgaacat
    20461 tatctagttg ataataatta tcaaaatcat tatattgtta taaaataata ctgtcaacaa
    20521 cctttttagt ttgaaaataa ataaaagagt aacacttata tgtaagtcat cactcttttg
    20581 tagtctgatt tatcatcctt cattatattc tgattttaat aaactataga tgtaactcga
    20641 tgaataaata ccatttagta attcattgtc acgtaacata ccttccctag taaatcccag
    20701 cctttcagga atagcttggc ttttttcatt attaactgcg gcactgatct caattcgatt
    20761 taattcaatc tcatcgaagc aatactttat caatgcctca actgcttgtg tcataatccc
    20821 atgaccctca aattgtttat ctaaataata ccctaatgaa gtttttctgt gcatgtgatt
    20881 aatttcatgt aaaccgatga caccaactag cgttccttca taccaaatgc cacactgaaa
    20941 tccattacca tcagcaaatt gcaaaagtcc acttttaata aatgcacgcg tatctgatgg
    21001 ttgctcggtt gcatctaccc aaggtaacca ttccctaagt gaatttcttg aacgattgac
    21061 taaattgaaa agcgcttctg tgtcatgggc ttctaaaatt tttaatctta tttgttcatt
    21121 cactttcatt ccaaacatac catcacaccc tcatttattt ttcatataat tctgtaaatt
    21181 atcataataa taacatatta atattacgcc ttgtatctta aatttgttgt ccttccccaa
    21241 cttgcattgt ttgtagaatt tcttttcgaa attctctatg ttggggcccc gccaacttgc
    21301 actgtttgta gaatttcttt tcgaaattct ctatgttggg gcctcgacaa taagcattgt
    21361 agtgcctatt actttggttg atgcccttga tacctatcac acaacttaat ttaatcacta
    21421 ctttattaac cattccaaat ttatatttaa aaatacgaca aaacagtgct aaaatgctat
    21481 taaaggaggc actgctcatg gcatcattga aaaataagca ttttatgatt ggactatcat
    21541 taacatttat agtcgcatta tttagctttt tagcagctaa attgccaatc ttagataagg
    21601 ttggcgcatt aactatcgcg atattaatcg ctattctgta tcgtcacttt aaaggatatc
    21661 ctgaacaata tagctcaggt attacatttt catccaaata tttattaaga tttgcaatca
    21721 tcctatatgg acttaaactc aatatattcg atattatcgg tcaaggtagt agactattag
    21781 ccatcgatgt aggtgtcgtt attttcagta ttgtaatgat gctctttgtc aacaaactgt
    21841 tgcgtggtga caaaaacatc gcattattac taggtgtagg tacaggcgtt tgtggtgctg
    21901 ccgctattgc tgcagtagct ccaatattca aatcacgtga aaaagataca gctattagta
    21961 tcggtatcat cgcattgatt ggtacgatat tttcacttat atatacagct atctatgcta
    22021 tcttttcaat gacgacaaat gtttatggcg cttggtctgg cgttagtctt catgaaattg
    22081 cacacgttgt cttagctggt ggctttggtg gtagtgatgc acttaaaatc gcacttcttg
    22141 gtaaacttgg tagagtattc ttactgattc cattaaccat cgtacttatt ttaattatgc
    22201 gcttccgttc atcagaatca tctagcaagg gtcgaataag cattccatac tttttaattg
    22261 gatttgtcat tatggcctta gtgaatacat atgtaactat cccgtcagta ttgttaaata
    22321 ttttaaatac tgtatcaaca atctgcttat taatggccat ggttgcttta ggattaaatg
    22381 ttgcatttaa agatcttaaa aatcgtgcac ttaaaccgct tatgacaatc attattacct
    22441 caatatgctt gtcatcactt gcctttatcg tcgttcactg gctatatagt taatatgaaa
    22501 agacgctcaa acacttacaa atttagtgtg ccgagcgtct tatttttata ctttaaaagt
    22561 cgtattggtt attttcttct tcaagcactt gttgttgtac tttggcagtt cgcattgtgg
    22621 attgtgccaa cgcttttaaa cgaaatacaa catcatcatt gacaatttgt tgatttgaaa
    22681 agtctttctc ttcaatgaat acatacgttt gcatcgtatg tgctttcatg taactcaata
    22741 ttggttttaa atgcatttca ggaattaaat aatgtttact agaacctgct gtcgctacaa
    22801 gtcctattac cttatcatga aacgcattga ctggaagtaa atcaaacaca tttttcaaag
    22861 aaccagggat ggaagcttga aaaattggaa aaccaataaa aatcacatca gcctgcatta
    22921 acgacgtcgt taatttatat acatctcctg tagtatctag ataattcttt ccaacactaa
    22981 attataattc gagatctcgt aaatccatca atgctatttg gtgtccctca ttgatgactt
    23041 ctagttcatt ttttaaatca tccatagcaa ttctcgtttt agaacctact gtggagcctg
    23101 acaataatac aatattcatg atgtcgcccc tcctattttg ataaatgctt tttaatcgct
    23161 ggtataatgt cgttgccaat taactcaata ttcttcataa cattttcaaa tggcacaccg
    23221 ccaaaatcaa gctgtgccat aaaacgttga tgaccatata actcgtgttg atacaatatc
    23281 ttttcaataa tttgttgcgg gcttccaacc attagcgctt ctcgataatc tgacgaatta
    23341 gcaaattgct gtttcggata accaacgcca cgaataaatg acatccctgt attcaaatgt
    23401 ggataaaatt ctctcatagc atcctgagtt gttttagctg tataaaacag actcgctgta
    23461 cttactggta aagacttagg cgaagcatcg aaacctgctt cagttgctgc ttgacgataa
    23521 gcatctatag aacctttaaa gttcattgct gggccaccaa gggttgtaat catcattggc
    23581 acaccttgtt ttcccgcttt gatagcactt gcaggtggac caccaacagc acgccatatt
    23641 ggcaatgtat tatcgattgg tcttgggaat attttcatat ttctaagttc tggacgatat
    23701 tttccagacc aagtaatacg ctcagtttta tttaactcta aaagtaaacc taatttttct
    23761 tcaaacaatt catcatagtc ttttaaatca tagccaaaca agtcaaaaac acctgttctt
    23821 gatgctctgc cagctacaat ttcggctcta ccatgagaaa tcaaatctaa tgtcgcgaag
    23881 tcttcaaata ctcttacagg atctgttgca ctaataatcg ttgaagaact tgaaacttta
    23941 atatgctttg tcgcttgggc aattgcacct aacacaaccg tatgtgcctg tgtcgtaaaa
    24001 tgttcctgat gactttcacc aactgcaaaa acatcaatac ccgcttcatc tgctaattta
    24061 cttgcttcaa taatttcatt aatacgttgc tcataactaa ctttttcacc tttaaatgga
    24121 ttcaataaat gatcacctaa ggaatacaaa ccaaactcaa gaggcgtatt tttattcatt
    24181 tctaattttg ccattaaaca ttctccttta tatttcgtgt aacttacttt caatttgttc
    24241 acgttctgct tctaaaaagt caggcaagtt caattgactt cctaattgtt caacaggtgt
    24301 atcaatagta aatccaggcg cttcagtcgc aaattcatac ataattgaat tatggcgata
    24361 gtataatgat ttaaagaaat agcgatcaat tatacctgaa ttactttgtg gttgttgttg
    24421 tagtttcttg taaattgcat ctaagtcatt catttgtggt gtattaactg caatatgatg
    24481 gatataacct cgtccaggtc tttcacgttg tccctgttgc tcaataacta caaaatccga
    24541 atataaacct tgtggtgcta atgtcagcac atcgaatgat ttattatctc ttttgcgata
    24601 acctaagaca ttttccaaaa attctaccgt acgcgctgca tttctaactc ttaattcgac
    24661 tggccctatt cctaaaattt gatatgcttg aggtatttca ctataagcgt tatgttgcca
    24721 ttgatgtggt acttcaaaac tatcattaac aagcagtatg atttctaagt cgtcttcatc
    24781 tttaaaaaca acaccttctt gtccaagata gtacaaacgt tcagacttaa taccattatt
    24841 ctcaagacgt ttttcaaaat aatgaagtgc cgcttgattt ggtactaata atgaaaatcg
    24901 ataaatcgtt tcagtacctg gctgcttatg acccgcattg ggaatttcaa aaaaacttaa
    24961 aattgttccg gctgtaccta cttcgtcccc ataaaacaaa tgatacattg aaggattgtc
    25021 ttgattaacc gacttttcaa ccaatcgtaa tccaaggaca tttgtgtaaa aatccttatt
    25081 acgttttgca tcttttgtat acatggatat gtgatgatgc cctactatat tcatactatc
    25141 cctcctatta gtataaattt tataccaact tatataaaaa agatagttat atgccattta
    25201 gcccttttct gcaaataaaa atatgtttgc gctccttcgt agatacacag ctagtatgta
    25261 taacgacata ataattgcat aattataaat ttatgtgtag caatttattt tttctaaatg
    25321 tttataaaat acttcaataa ctaaatatgt aagatttcat aaaagtatat tgacaacgct
    25381 ttcatacatg tctaatataa gtttatcgca caaaatgcat ataaatacat attaaggagg
    25441 attttatgaa ttttcttaaa cctgcaaagc atattaagcc tttgccagaa aatcagatag
    25501 atgataccta taaacgatta cgtctccaag tatttcttgg tattttcatc ggttacgctg
    25561 ggtactattt attacgtaaa aacttttcat tagcgatgcc ggcattgcaa gagcaaggtt
    25621 ttacaaaagc agaactaggt tttgcgcttt ctgctgtttc catcgcatat ggatttagta
    25681 agttctttat gggtactgta agtgatcgga gcaatgcacg gatattctta gttcttggat
    25741 tagtactcac tgctatcgtc aatttgttaa tgggatttgt accgttcttt acatcaggta
    25801 tcggtattat gtttgtccta ttattcttaa atggatggtt tcaaggtatg ggctggccac
    25861 cttcaggccg tgttctcgtt cactggttta gtgtaagtga acgcggaagt aagactgctc
    25921 tttggaacgt tgcgcataat gttggtggag gtattatggc acctattgct gcttggggta
    25981 ttacaacaac agcatttatc aactttggtt atttaaaagg ttttgaaggt gtattcattt
    26041 accctgcact cttagcactt atcattgccg caatttcata tatattgatt agagacacac
    26101 ctcaatctca aggtttacct ccaatcgaaa tttataaaaa tgactttgct acaagcgata
    26161 agaaaacatt agaaacagaa ttaactacaa aagaaatttt atttaaatat gtactgaaca
    26221 ataaatgggt atgggcaatt gcctttgcaa atatatttgt ttatttcgtg cgttatggtg
    26281 tacttgattg ggcgccagtc tacttaagtg aagaaaaaca tttcgactta aaagcatcag
    26341 gttgggcata cttcttatac gaatgggctg gaattcctgg tacattatta tgtggttaca
    26401 tttctgataa attattcaaa ggtcgccgtg gacctgctgg tttcttcttt atgttaggtg
    26461 tcacagtatt tgtattaatt tattggttaa atcctccagg caatgcttgg ttagacaatg
    26521 tctcattaat tgccattggt ttcttaatat atggaccagt tatgttaatt ggtttacaag
    26581 cattagatta tgtacctaaa aaagcagctg gcacagcagc tggattaact ggattatttg
    26641 gttatctgtt tggtgctgta atggccaaca tcgtcttagg tgctgtagtt gataaattcg
    26701 gatgggatgt cggttttatt ttattaacag caatcagtgt gtttgcaatg ttgagcttta
    26761 tcctcacttg gaataaagta ggacaagaaa ctgttcatca ttaaatgata aaaaataaag
    26821 tcatatggtt atcttatcga aagatgatat atacatctct tataagttca accatatgac
    26881 tttttaatta gtattcaaaa aaatatttac attgccactt ttgtgtttgc tctgctcttt
    26941 tattcaattg attacaccac ttaggataaa ctctaaaagc cattttacct ttatgctcaa
    27001 atgtagttaa gatgcctttt tctaccaata attccctagg aaataagaaa taaccgctta
    27061 attcttcatc aataactata atcattaagt aatctgcaaa tgcctctttt gagtatggtt
    27121 gattacaatt gttctcgtct tttgtccaac atgtaacaaa ataccctgct ttagtcggtg
    27181 ttttctttgc taacctactt tgatatgttt cctctttaaa gctaaatgtt aatgcttcgt
    27241 aatcttgatt atatttttct tccgttaaat ctttaacgtc tgattcttca ctaaaaatat
    27301 atttcaacag tattttagat ttatacatat gccaatctca ctttattttt tcataatcgt
    27361 atcatatatt tattttttat ttgaaaaata cacttccgac cgaaatatac tcagccagaa
    27421 gtgtacgaaa tatttaacct tctattaatt cagatgtatc aactgtcaaa cgatcacgta
    27481 ataaatagac gattaacatt gctgctaaag cacaaagtga ctcagcaatt aataatgacc
    27541 aaatgacacc tgttagtcca aacaaagcat tcataataaa tagtactgga atgataacca
    27601 caccttgtaa aatggccata attgtagcac cgcgaccttg accagttgct tgaagcatac
    27661 cagtaaacaa gaaacctata ccatttaata acaatgatgt cattgttact ttcaaaataa
    27721 atgtcgccat ctcaacaatg gcttgatcag tagtaaatag tccgaccata tgatgtccaa
    27781 ttgtaaatac cgcaatcata catacaacaa agataacgcc gatagacatg ataactgctt
    27841 tgataacgtc tttcatacga cctttatttg acataaagtt atatgcaatt aatggtacaa
    27901 caccttcaca taagcccatg ataataagtt ctggaaattg cacaagtcta aatgagatac
    27961 cataacttgc aatcgcgaag tttccataat gtgctaaaaa taaatttaaa actaatcctg
    28021 tgaatcccat taagatactc attaaaaatg caggaatacc gattttaaag atttcagaaa
    28081 gcatttcttt attaggtttc gcaagtttaa tattaactga cacaacgtca ctatttttca
    28141 taaaataaat gataaagaac agagcagcag caacattact gattgcagta cccaaagctg
    28201 caccaacaac gtttaaatca aaaccaaaaa ttaaaattgg atctaaaata atatttaagc
    28261 ctacactagc taacatacca atcatagaaa tcattggtgc cccaattgca cgtgcaaatt
    28321 gttctaatat gaagaacaaa attacaaaag gtgcacttaa aaacattact ttcaaataat
    28381 tacttgttaa agctaacgtt tcacctctcg cccctaaaat tgctgcgatt tgatcactga
    28441 atggtaaagc aactaaaatt acgataagtc ctagtgcaat accaccataa atagagaaac
    28501 tacttacaaa tttactctta ctatagtctt tcgcacctaa taaacgtgaa atataagttc
    28561 ctgcaccaac gccaaataaa ttacccaacc ccattaagat agcaaatact ggcagtgtta
    28621 gtgagatagc agaaatcatg tggctatctt ctaaaaatcc tataaagtaa atatttaata
    28681 tgccataaat aacgcttaat aaagtcccta tcatcattgg caatgagaaa tgcatcatcg
    28741 ctttaaatac tggcgatttc tcaaaataat ataattgttc gtctttcatt gttcaatact
    28801 ccttgtcttt tccaataatt agcttactaa caattagata tctaactata acattaaaga
    28861 taaagtgact gatttctacc agtcacgctt accatttatt gtaaactaga taacatttta
    28921 gttaagtttg ctttcatctg ttcattttct tcttcagata actgcgatac gagtgtttgc
    28981 tccatttcat caaatatcga agtgaatgct tctacaagtt taatcccaga ggtagtcagt
    29041 cctatattct ttcttctcgt atcctgtgca tcgacatagc gatagatcag ctttttacgt
    29101 tcaaggttcc ttaataaatt actgacagtt ggacctgttc gttgtaatgc tttagcaata
    29161 tcattttgtg tcagcccatc ttgttgatgt gcataaagat aacctaacgt atgcccttgc
    29221 tcatttgtaa tatcaaattg ctctaacttt tgatcagcct tttgtttcat ctcatgacta
    29281 atcattctaa ataaatacga ataagtgaat tccatttatt gcaccaccat ttcgttagat
    29341 gtctaactaa atatactgcg ccatttttaa attgtcaaca tgaatatcta atttatttta
    29401 aaagttccta taaaatcttg cgccataaac attttgatat gcaatttttc ggattaaaat
    29461 atgaaagaga ttgcatatct acgttcttga gaaatcgctt acaattacaa ataggaggat
    29521 attacgttgt taagttatag acaaattgaa attttgtatc ttttaattca tgagcaaacg
    29581 tttattccaa tcaataccat tgctaatcaa cttggtgtat cacctcgtac cattcaatac
    29641 gatatagcat atattgagca atatgctgaa acttatcatt accaagtcag ccgtaataaa
    29701 gctgcaggca tcaaagtaac aacagctaac acaacgatat taaatgaatt agagcacaat
    29761 ctaacgaatc aaattcattt ttcaaaagat gagcgactca cacatatcgc tttaaagtta
    29821 ttcgaaacaa ctgatcctgt ttcaacaaag caacttgcgc aagatgttaa tgtttcgcgt
    29881 cggacaattg cagatgatat taaaatgatt caagcccaac tagatcaata ccatttaaag
    29941 ttaaattatg tccacaataa aggattcaat attattggtg aggaagatca ttatcgtaaa
    30001 gcgtatgcac actttataca tcaatatatg aaacaagctg caccttttat agaggctgat
    30061 atctttaatt cagaatcaat cgcattagtt cgccgtgcca ttattaagac attaaatagt
    30121 gaaaattatc atttagttca gtcggctatc gatggcttaa tctatcatat acgcattgcc
    30181 attcagcgtt taaatgaaaa tttttcgttc gatataccta tcaatgaaat tgataaatgg
    30241 cgacatacta atcagtatgc tattgcttca aaaatgatag aaaacttaga acgcagttgt
    30301 aatgtcacat ttccagagtc tgaaattatc tttatcacgt tacatttact aggctctaaa
    30361 atgaccgaac atacagcatc ttcaattacc tttgaatacc atgatttatc gcaaaatata
    30421 catgaattga tcacttgtgt tagccaagaa ttaggcattg atatgtcaaa agacaacaag
    30481 ttgcatacca gtcttatcac acatatcaaa ccagctatac atcgtattaa atacgatatg
    30541 ctacaaccta atcctttgag gcaagaagtt atgcgtcgct atcctcaaat cattgaagcc
    30601 gttagcaagc atattagccc aattgaacaa gatgctgcta ttcgcttcaa cgaagatgaa
    30661 ttaacataca ttacaattca cttcgcatca agtatagagc gtgttgcaac acataaacaa
    30721 tcaatgatta aggttgtctt actatgtggt tctggtatag gcacgtcgca acttttaaaa
    30781 tcaaaactaa atcatctgta tcctgagttt cacatttggg atgcctattc catttatcaa
    30841 ttggaagaaa gtcgattatt gcaagataac attgattatg tcatttcaac agtaccttgt
    30901 gaaatctcag ctgtaccagt tattcatgtc gatccattta tcaatcagca atctcgtcaa
    30961 aaattgaatc aaattatcaa tgactcaaga gaacaacgag tcatgaaaat ggcaactgat
    31021 ggcaagtcac tcgcagattt attgcctgaa catcgcatca ttataaataa acaaccatta
    31081 tcaattgaat cggcaattac agtggctgtg caacctttaa tcaatgatgg cattgtcaat
    31141 tcaaattata cagctgcaat tttaaaacaa ttagaacaat tcgggtcata tatggtcatt
    31201 agtccacaca ttgcacttat tcacgctggt actgattatg tacagaatgg tgtaggtttc
    31261 gcactaactt atttcactga agggattatt tttggtagta aagctaacga tcccgttcac
    31321 cttgtaatta cattagcaac ggaccacccc aatgcacatt taaaggcatt gggacagtta
    31381 agcgaatgct taagcaacga cttatgtcga caagatttct tagatgggaa tatttttaaa
    31441 attaaacaac acattgcttt aactatgaca aaggaggttt aataacgtgt cattagacat
    31501 tttgtcaaca acacgcatca ttgtaaaaga acaagtaaat gattggactg aagctataac
    31561 tatagcttct cagccattac tacaagaaca aattattgaa caaggctatg ttcaagcaat
    31621 gattgatagc gttaatgaac ttggacctta tatcgttatc gcacctgaaa ttgcaattgc
    31681 acatgcaaga ccgaacaatg acgtacatca agttggttta agtctattaa agttgaatca
    31741 acatgtggca ttttgtgatg aagatcacta cgcatcactc atttttgtat tgagtgccat
    31801 cgacaatcat tcacacttat ctgtattaca aaatttagca accgtactgg gcgataacca
    31861 aacagtccag caactattaa ctgcaacaaa cgcacaagac attaaaaaca ttttaaagga
    31921 gcatgattaa tatgaaaatt ttagtagtat gtggccacgg tttaggaagt agttttatgg
    31981 tagaaatgaa tgcacaagaa gcgcttaggc aacttaatgc accatctgat atcgaagttg
    32041 aacatagcga cattatgaca gcaagtccag agatggctga cttgtttatt tgtggtagag
    32101 atttagctga aaatgccgaa cgtctagggg atgtcttagt tcttgataat attttagaca
    32161 aagctgaatt acaacaaaag ctctcagaaa aattacaaca acttaacatg atttaaagga
    32221 ggtacgacct atgcaagcaa tccttaattt tatagtcgat attttaagtc aaccagccat
    32281 tcttgttgca ctgattgcct ttataggttt aatcgttcag aaaaaacctg ccgcaacaat
    32341 cacttcagga accattaaaa cgatattggg cttcttaatt ttaagtgcag gtgctgatgt
    32401 cgtcgttcga tctcttgagc cattcggcaa aatattccaa cacgcattcg gtgtgcaagg
    32461 tatcgtacct aacaacgaag ctatcgtctc actagcctta aaagattttg gaacaacagc
    32521 tgcactcatc atggtctgtg gcatgattgt taatatttta attgcccgct tcactaattt
    32581 aaaatatatc tttttaacag gtcatcatac attttacatg gctgcgtttt tagcaatcat
    32641 tttaacagtc agtcatatta aaggctggct aacgattgtt atcggcgcac tcgtattagg
    32701 attaatcatg gcagtcttac ctgcattact ccaacctacg atgcgaaaaa ttacagggaa
    32761 tgaccaagta gcgttaggtc attttggctc aatcagttac tttgccgcag gtgctgtagg
    32821 tcaattattc aaaggtaagt ctaaatcaac ggaagagatt aaatttccaa aaggcttaag
    32881 tttcttacga gaaagtacaa ttagtatctc gattacgatg gcattacttt acttcatcgc
    32941 atgcttattt gcgggcgtta gttatgtaca cgaatctatt agtgatggtc aaaactttat
    33001 tgtattttca ttaattcaag gtgtgacatt tgctgctggt gtatttatta ttttaacggg
    33061 cgttcgctta atcttagctg aaatcgtccc agcatttaaa ggcatttcag aaaagcttgt
    33121 accaaattct aaacctgcat tagactgccc tattgtgttc ccttatgcac aaaatgcagt
    33181 actaatcgga ttctttgtca gctttattac aggtgtcatc ggtatgttta tcttattctt
    33241 atttggtggc gtcgtcattt tacctggcgt agttgcacac ttcttcttag gtgcaacggc
    33301 tgctgtattc ggtaatgcaa gaggcggtat taaaggtgct gttgctggcg ccgctctaaa
    33361 tggtatccta ataacgtttt taccattatt attcttgcca tttttaggcg aattaggtgg
    33421 tgctgcaaca acattctcag atacagactt tttagctgtc ggtatcgtgt tcggtaacgc
    33481 agtaaaatat atgggattat ttggtgcgat tctatttatt ataatcgtag gtgcaacagc
    33541 aattttatta aaaggtcgtc aaaagccaca gcaatagtgt taacgtaaaa atataaaaca
    33601 ccgtcacata ttgagtgaat gcccctttta tcaagaggaa agccacttac ttatggacgg
    33661 tgtttttgta ttatattaaa tgaaatttag ccatactatc aattgctgct aaaattgctt
    33721 cttcttgtgt cgcaatcggt tcccaaccaa gtaatgtttt tgcacgttcg ttacttacat
    33781 tgcggttcat atctaataaa agtttacctt ctttagcttg atgattaaat ttagcaccta
    33841 gactcaaaat aaagtctggc aattttttag tagcaacttt ttgagctatt tcaggtcgtt
    33901 tttctttaat taattttgca atttccaata aatcaatttg cccatcagcc gtcgcaataa
    33961 atcgtttacc gctggcttgc tcattggtca ttgccaaaat gtgtagttct gctacatctc
    34021 tcacatcaac aacatttaat ggaatttgag gtacacgttt cattgaacca ttcaataaat
    34081 tttctaataa atgaaagctt cctgaaacat gtgcatctaa tgatggtcca aaaattgcaa
    34141 ctggattgat tgtggcaaat tctactgtcg tattttcatt ctcaacaaaa tcccacgctg
    34201 ctttttcagc taacaatttt gatttttcat atactgataa gcctggttca tcttcatttg
    34261 tccaatgact ttcatttgtg attgaatttt tatctttatt actaaaacca actgcaccaa
    34321 agtttgcagt cattaccaca cgtttaacac ccgcatgttg tgcagctctt aaaatacgtt
    34381 gtataccttc aattgcaggc ttcgccatca cttctgcatc gtctgtttta ccgaaaaaca
    34441 ccggagatgc cacactcaac acatatttac aatctttcat tgcttcatcc caatgttcat
    34501 cttgtgataa atccgcttcg acaaacatta atcgctctgt ggaaatgcca ttgtcttgca
    34561 tcgttttaat tactttatca gctttactta aatcacgtat cgtcgtttgt acctcataac
    34621 cttgttctaa taatcgtgaa ataattcgca tgccaacaaa accagtacca ccggtcacta
    34681 atactttatt attcatcaca taatctcctc tttgtgtcta ttgattattc attatagcag
    34741 tcattatatt ttgacgtata taaatagact ttggtagcta ttatttaatc ttacttgtat
    34801 aaatgaaaat gaatttattc ctttaaaaac tatcattaac cattttcaaa tacccaatca
    34861 ctaataaaca caaacgctat aaatatgatg tctttgctaa ttaactaaat tcattttcaa
    34921 tcgtatgttc aaatcacata actacatagt caccatttta aaagcaaata cacatgttca
    34981 acgagagtgc gacattaata cctaaacaat attaacttaa aatcatctca accaatgatt
    35041 caaccgtaac attaccaaaa taataaacga tgtctaattg ctttaactga tgcgttaaat
    35101 cttctcttgt catttttgtt ccttgtaatg cttgttccac atcttttata tcaccttgtc
    35161 caaagaaatc accaaaaatt ctacaatctg caatacgatt ttgttcaaca gaaatggtaa
    35221 tatctacagt acctgatgat aatctttcac ttcgattata ttcatattta ggtgacttcc
    35281 cataattcca atcccaattt ttatactttt tactcgccaa ttcttcaatc gattcccaat
    35341 ctgcatcaga taattcatat cgtttcgcct ctttaatgtc atcgattccc aaaatctgcg
    35401 tcaccattaa atttttaaat tcttcaatgg ttatatccct atacttttca tccagtgctt
    35461 ctctaagatg accaacacgt gcacgcacag atttaatccc tttcgatgca atctttttgc
    35521 gattaggctt taacacttta tccattgctt cataattaac atcaagtaat agcgaataac
    35581 cgccataaat acgattgttc attaatgtca tggcggcgcc tgacactttt ttgccgttca
    35641 atgttaaatc atttctaccg ctttgtatca catctcttgc acctaatgta tgcaacgcct
    35701 ttatagctgg ttgataaaat cgttgaaaat caccataaat tgaagtgtct tgttctaaaa
    35761 tacaacacat attaacggca cctttatcaa catacacagc gccaccacca gtatctcggc
    35821 gaactacttg aatactgtgt ttatctatat aatcttgatt cacttctata gcagtatttt
    35881 gaaaacgtcc aatttccact tttggatcac aataataagg gaataaaata tcttcatcta
    35941 aaaagatatt ctggttaaca taaacttgca tagcgagtgc aatcgcacca tcagtaatat
    36001 attctccatt cctaatcggt tctattaagt acatcttttg tctctccaat gttgttattt
    36061 tgcgttgtgc tatcgttccg gagtgctgtt gtaatcaatg ttgagatatc ctcagttaaa
    36121 tgtatggtac gttcttgaat tgaattcgga atgcgatatg cctttttatt catcgtcata
    36181 taaagggcat tttcattttt acgtgtcata cgctgaaaag gatgtttcac aaattgtggc
    36241 gtagtataac caattccaat ttccaaatac aacactttat catctttatg ttgctctaga
    36301 aaagcattat aacgttgtag ttgcgcatga aattcagcat cttcaaccat gccaacttcc
    36361 gctttacgtt tattcacttc cattggggca tcacatttgg gacatcttgg aatcatctcc
    36421 caaggtataa gcatatcttg ttgcgcaaca accattttac gaattaaatc atcattgcga
    36481 tacgtttgag catgacaatg ctgactacac tgttgtagta tatactcgcc ttgtatatga
    36541 aatacatgcg tcatatcata ttcagcagca tcgaaagcat tgtctgcatt cgtagttatg
    36601 atatggtact gtttatcttc cactaaggat tttaatgcga gataagactg acctacaggt
    36661 tgatctaaat agtttaatgt aataaaacga ctctcaaatg cccaatactc ttgccaactg
    36721 ccataaggat gtaaactcgc ttgcaacata tcaaagaagc gatatttttc aataaaatct
    36781 gggaaatttt ccgtaaaacg ctctcctaca tatgtaaacc cgtcagatgc agacatgcct
    36841 gcaccaattc caatcactat cgcatctgct tcatcaatcg cagtacgcaa tacttcagcc
    36901 tgctttgtct tgtcatccat taaaagagac attgcattcc atttactact ctgcatcacg
    36961 gttaaatgcc tccttatata gttgtaaatc cttatctgta aatacattga acacgacttt
    37021 caatgttgaa tttgtttctt tgagatagct ttctactgtt cgaacagcaa tttctgctgc
    37081 ttcatcttga ggaaaagcaa atacacctgt agatatacag caaaatgcta tatgatttaa
    37141 actttgttga tcagccaatt taagacaact caaatagcat ttagctaaca agtcctgatt
    37201 caactttgaa acaggcaatc gacgtatttg cggaccaacc gtatgaatta tatactttgc
    37261 tggcaaatta tatccacgtg ttattttggc tttacctaca ccttcattgc gcccttgttg
    37321 tcgaataatc tctgcacaat caagtcgaac ttgaacaccc gcttttgtat gaataatatt
    37381 atcaatgcag tcatgattag cttgcataca tcctagaaag cgactattcg cagcattaac
    37441 aattgcatcg atttttaacg tcgtgatatc accttgccaa acaaagatat tgtcaccttt
    37501 tattggcttc aaatctttca aatccgtaac atgttgacga ttcaaatcac ttaacatagc
    37561 atcttgtaca gctaaatatt catcacttac aggtaatgcc ggcctgacat ttgctaaacc
    37621 tcgatataat tcccacaaat cttcaaatga agatggcatt accaatacgt cattgtcatt
    37681 tctctctcga cgcatatcat tgattaaata ttcaagtctc gctttatttg attttaacgt
    37741 ttccatttaa gcctccggta atgccaggaa tgcttcttta tcgacatcat ccaatttgaa
    37801 caaccaattt tcttctggtt tttcagagtt taaaattgtc ggttcttctt ccgcttttgt
    37861 atttcgctca ataatcgttc ctgacaatgg cgtttgcaca tcaatgaccg ttttcgatgc
    37921 ttcgatactc acaatttcat catccacttt aacttcatct ggactcacga attcaacata
    37981 acctactgtc ccaatatcat cttgcaattc aggtgtcata ctaaacacat acaaatctcc
    38041 tactttttct acccataaat aattggctaa ctttttcatc ttatcttcat cctttctctt
    38101 ctatttaaaa tgctacaaat agatttccgc gagtaatttt aatgttttac aacgctgttc
    38161 aatgcctgga ataagcggtg ctactaacac ctcatcaact tcaaacgtag caatgaaatc
    38221 atctaattgt gctttaacct gttcttgtgt acctgcaatg atacgtgctt gatgtgcttg
    38281 aatcatctct ttgtctcgat cattgagctt atacttttgc gcagtgtcta ccgatggaaa
    38341 atgttcaaat tctgcaaatt gtaatttacc taataaccaa acatctaagg catgttgtaa
    38401 tgctgctact tccgcttcgt tatcagctac aatgacaaat acagatgcca tcacctttgc
    38461 gtccatttta atcgttgatg cttggaaata ttttttgtaa atatcaatgt tatccttcgc
    38521 tgcatgtatc gcatttatat ctggtagcaa aaatgttcca acagaaagcc ctatacctag
    38581 ttcggcagct attttggcag atgtttcgct actacttaat aaccacattt ctggaaaatg
    38641 atcaatgtgt ggttggacac ctaacgtatg cgcacttggc ttatccttta ttgtaagata
    38701 atcacgtaat aacgaaatcg attcatcgta actatcatat gtaggattta ttccatctaa
    38761 agcttgcttt accatagtag tacccggatt attaccaata cctaaatcaa tacgatttgg
    38821 atataacgct gccatcattc taaaatgctc agcaatttta taaggtcgat agtgcggcag
    38881 catcacacca ccagaaccaa ctcgtatgtg atttgtctgc gccaatgtat gcatcatcaa
    38941 aagttctgga ctactacacg caaacgctgg tacattatga tgttctgtaa accaaattcg
    39001 cttaaagcca agtcgatctg ctaattttgc aagtgtcact gaatcctgca acgccttttg
    39061 tgcatcctta ccttcatcta ttaaggcata gtctaatacg cttaatttaa ccaatccgtc
    39121 atctccaaac ttatcctgtc atatcaaacc gacataacat tttaacgttc ttaataccat
    39181 tccctcttca tatacccacg tatatgataa cgtttttcaa ttaactttta tatcttttcg
    39241 cttgtatttt tctttttcat attcatattt tcagacctac aaatgacatt cattagttca
    39301 attttaattt taaaaatgct ttccatgtac caattctatt ctatcaagac tgttcatcat
    39361 tgattattca ttctttccat ggtactttga aaacaatatt gaatagaaaa ggaatcatag
    39421 tatgtaccga tacaaaccac ttttacaatc aatccactta cctaatggca tcaaaatttc
    39481 gaatcgcttt gtattatcac caatgaccgt aaacgcatca acaaaagaag gctatattac
    39541 aaaagcagac ttggcttatg ctgcacgtcg ctctaattca gctggtatgc aagtcaccgg
    39601 cgctgcttac attgaacctt atggtcaatt gtttgaatac ggctttaata ttgatcatga
    39661 tgcttgtatc cctggattaa ctaacatggc atctacgatg aaacaacatg gtagcctcgc
    39721 tattattcag ctagcgcatg ctggtcgttt ttcaaatcaa gcaattttaa attttggaaa
    39781 ggtgtatgga ccaagtccta tgacgttgca ttcaccgatt gaacacgatg ttatcgccat
    39841 gtctcatgag aaaatcaata gcatcattca gcagtatcgc gatgctacat tacgcgcgat
    39901 taaagcaggt tttgatggtg tggaaatttc gattgcacaa cgtttgctga ttcaaacatt
    39961 tttctcaact ttttcgaata aacgtacaga ccattacggt gccgactcat taaaaaatcg
    40021 tgcaagatta tgtttagaag ttatgcgtgc ggtacaagaa gtgattgata aagaagcacc
    40081 tgacaatttt attttaggat ttcgagcaac gccagaagaa accagaggta gtgatttagg
    40141 atataccatt gacgagttca atcaattaat cgattgggtc atggacgttt caaacatcca
    40201 atatttagca atcgctagct ggggtcgaca tatttatcaa aatacgtctc gtacaccagg
    40261 tgaacatttc ggtcggccag ttaaccaaat tgtctatgaa catttagctg gtcgcatccc
    40321 tttaattgca agtggcggga ttaattctcc agagtctgct ttagatgctt tacaacatgc
    40381 agatatggtc ggcatgtctt cgccttttgt cacagaacct gactttgtgc ataaactttc
    40441 tgaacagcga ccacatgata ttgaccttga attttctatg gcagatttag aaaatttagc
    40501 cattccgcgt gccgctttta aagatattgt taaaatgatg gattatgggg aaggtctaaa
    40561 gaagcatacc cgtgacgctt tacgtcaatt agaacagaat tatcatgaca catcatcaaa
    40621 agataatttt tcatagaaat ttaagacaat gttataatga catcaatttc aaaaataagg
    40681 agtctgtata gtcgtgactg aaattaaaga taaagtaatt actaaagatg catttgaatt
    40741 accctacaca attattaaag cgaagcatca accaacaaaa ggtgccattg tctacattca
    40801 tggtggcggt ttaatgtttg gaaaggccaa tgatttatcg ccacagtata tcgatatcct
    40861 tacggaacat tacgatttaa ttcaactaag ttatcgttta ttaccagaag taagccttga
    40921 ttgcatcatc gatgacatct tcacatcatt cgatgcgatt caatcacaat attctaattg
    40981 ccctattttt acctttggca gatcatcagg tgcatattta agtctattaa ttgcacgaga
    41041 cagagatatt gatggtgtta ttgattttta tggttatagt ggcatcaata ctgaaccgtt
    41101 taaaatggca aatagctatt acgctaaaat agctcaaagt gtgaatgaaa cagtacttgc
    41161 tcaacttact tcaccaacac cgattattca agatcaaatc gcccaacgtt ttttaatcta
    41221 cggttatgca cgtggtacag gtgagtggat caatatgatt aacattgctg attatactga
    41281 ttcaaaatac aacatctcac caaatgaact taaaacactt cccccggtgt tcattgcgca
    41341 ttgtaatggc gattatgatg tccctgttga agaaagcgaa cacatccaca atcatgtgcc
    41401 acactcaaca tttgaacgcg tgaataaaaa cgagcatgat tttgatcgta gacctaatga
    41461 cgaagcaatc actatttatc gtaaagttgt tgatttctta aacgctataa caacggcgta
    41521 atataccatt atttttaaaa acccaattta tcatcatgat aaacagttat tcaatcaacc
    41581 atattattca tacaatttat taagctcata aacagatacg cctgtccctt tagttttcgc
    41641 taatgagaca ggcgctttat ttcactttac tattttaaaa ttcaacactg tgatgctact
    41701 tatatcttcc gtgccatgtt agtagcacaa aatacatagc atattaaatt acgaatatga
    41761 atttaacttg ctttcaattg tgttccttta ctttcagtcg cttcaacacg caacaagtca
    41821 tttataatac ctgtatagaa gttggcgagt tctgcacctt tacgcttgaa atccaggaaa
    41881 tctacaccga taaaatcgac atgatcccat ccttgtatga ttggtttaac ttgccagata
    41941 cctcttcgtg tggcaggttc atcattcgta acattaacaa atggttgatt ggacggatgt
    42001 aatgacgaaa ttactggtac gacaccatca tttttacgcc attcttctct tgcatcatga
    42061 ccaataattc tactcgttgt atccattaag aaaaatgtac ctaaatcagg attttcataa
    42121 cctaatggac cagtatgaga tgatacacca gtataagtcg tgtacgtaat atttggattc
    42181 atacttgtca tgttgttcaa ttttgcagag ccatttaacg ttaaatcata cgcagcatta
    42241 tcgtctgatg tccaaatttt gcttttacta acgcgtttta tatagtcaat gtaactctca
    42301 tttggtaatt gtttaaagcc ccattgcgtt aatcctaaat cgatattcga atacttgtta
    42361 cccataaatc gatttaaagc gaacatgatt tttctaacag cttctgtatt tccaaactta
    42421 tcagctgctt gtgaaccatt atgtggtgtt gctaatgttg tgattgatgc aaccatattg
    42481 ttatgaccac cagtgaataa tggtgatatt tctccaccat gcgctttatg ataggcaatt
    42541 tcttctttgt taccatttct taaaaactct tccattaaac gaattgtttg accacccata
    42601 ctatgcccta caagatgtac ctttttacca ggttcccaat taggcatgat tcctttatat
    42661 gtcttaccat agcgttcatg tccgtattta gctgcatgtg ctgcgccata atctacgcga
    42721 ccacctttga tataataata aagttctaca gcacgatcat agttgctacc aaatgcacta
    42781 acacttgctt gatgtacatt atagccttgc tttctcaatt cttcgataac tttatattta
    42841 ttcccacccc aataatttgg gtataaagca ggtgcattat cgcctactaa tcctaaaaat
    42901 ccatgtacaa aaacaactgg atatttgtta agtggttgta cttgattcgc tttaagtggt
    42961 cgaacctttt tagcatctgc tgctgcattt gtagtcgaac gtgttttagg cgcttgaagc
    43021 gcatctttga ttagttgttt attatcagta tttttatctg tcggtttctc cggggaattt
    43081 tgaccatcga tatgtttact taaatcgcca acttgtggtt tctgattact ttgatgaaca
    43141 ttatcatctt gctttgcatc tatgatttga tgcgcgggtt gatgtgtatc ttgttgattt
    43201 ggatgtttgt ccgtggttgc atcttgttct tttgttgatt taggtgcagt tttatcatta
    43261 gatgggggtg ttgttgaagt tggtgctgct ttatcatgat ttggtattac cttttgtgcc
    43321 ggaatggatg gttgtgattt atcaacatca ttcgattgtg acgttgtcga ttgtgatcca
    43381 ttacgttcag catgatgggt attttgaatc tgatcatttt ggcgttcagt tttgttagtc
    43441 gcattatttt gatgtgactg tttagaacct tgtcgttcag aatcttgctt tacttgatta
    43501 tcattttgcg ttctgattat tttactattt tgtgttgatt gatgcttcga tgattgaagc
    43561 atatctttac cttctgtcac tgttccactt ttaccattct ctttatgcat atcgtctaat
    43621 tgctttcctg actgcatttg atgtgacgtt atcgcattcc cttgttctcc cggttgattt
    43681 agtgtttctt tttgtgccgc tgcattagtc gttggtattt tttccgaggc ttgtgcttca
    43741 tgtgatgtca caacaaacat tgtagccgct aacactgaca ccacgcctat tgaatacttt
    43801 ctaatactat actttctttc ttcttgtcct cttaacatat aatcacctca aaagtatttt
    43861 tttgatcccc tcttgagaat gaaaaacaac tgcactttca atataacatg acatatatta
    43921 ttttttgatt aagactacaa aattaataaa aatttaataa tacgatatta aatcgatact
    43981 aattatttac aaatatctgt atcctcaaat aatttataaa tactttaatt gtgacgttga
    44041 atcaacacaa caacatttaa gataaatatt ttattaagct atatagtttc aatctatata
    44101 taattagtta atttgtattg gtctgaaaat aatccaaact atacaattat agtaagtttt
    44161 cagatttttc taaattaaaa caatgattca tctcattaag gggaagatgc atcatacagt
    44221 tttgtgtatt catttaaaca aaggggttgc tttaatgaat caatatcatt ctaatgcaca
    44281 acaaccaagt gcatggcgtt tttttgtcta tagtttagtg ggcatactat gtttctttat
    44341 tccttttacg attaatggta acaacactat tttcgtcgat catgttcatc tagccattcg
    44401 ctcaatcata ggtccactta tgccctatgt tgcactgatt atgattttaa ttggtacagc
    44461 gttaccaata gtgagacgta cttttatgac ttcaatcaca aacttggtca ttacattatt
    44521 taaagttgca ggtgcaatga tcggtataat gtatgtattt aaattcggtc catcaatact
    44581 atttaaagct aactatggtc cgtttttgtt tgaaaaatta atgatgccat taagtatctt
    44641 aattccagta agtgcaattg cgctttcttt attagtaggt tatggcttat tagaatttgt
    44701 cggtgtttat atggagccta ttatgagacc tatttttaaa acaccaggaa aatccgctat
    44761 cgatgcagtg gcttcgtttg tcggcagtta ttccttagga ttattgatta ctaatcgtgt
    44821 ctataagcaa gggatgtaca acaaacgaga agccacgatt attgcgactg gcttttcaac
    44881 agtttcagta acttttatga ttatcattgc taaaacttta ggattaatgc ctcattggaa
    44941 tttatacttt tggataactt tagtcatcac atttgtcgtg actgcaatta ctgcatggct
    45001 accgccaatc accaatgaat caacagaata ttataacgga caagaaggag aacaagaagt
    45061 tgctattgaa ggaagcagac tgaaaactgc atatgcagag gcgatgaaac aaaatgcatt
    45121 aacaccatcc ctcgtgaaga acgtttggga caatttgaaa gacggtttag aaatgactgt
    45181 tggtatttta ccttctatat tatcgattgg ttttttaggg ctgattgtag cgaactatac
    45241 accattcatt gattggcttg gctacatatt ctatccattt atttatattt tcccaattgc
    45301 tgatcaggct gtacttgcaa aagcttcagc gatttctatt gtagaaatgt ttctaccatc
    45361 tttgttagta actaaagctg caatgagtac taaatttgtc gtcggtgtag taagcgtatc
    45421 agccattatc tttttctcag cattagtgcc atgtatacta gcaactgaaa ttaaaatacc
    45481 tgtctggaaa ctcatcatca tttggttttt acgcgtggca ttgtcgctat taatcaccat
    45541 ccccgtcgct ttacttattt ttggataaag ttgaaaataa aatcactatc ataaacaaac
    45601 aaaacgagat gaacccatta cattcattca gccacctaag ataattaggt acctttaatt
    45661 aatgatggtc atctcgtttt ttatcgttat catttaatct tccataattt gaacaaaacg
    45721 tttcgtaatt tcttttggtc gtgttatcgc accaccaacg actgaacaat gaacgcctaa
    45781 gtccatcacg cgtttataca tatccggtgt aatgacatta ccttccgcaa taacttttgc
    45841 atcaacactt tgtagtacat cttttagaaa ttggaagtca ttttgataaa gtaattgtcc
    45901 ttgcgtataa ctagtatagc catgtaacgt cgtgccaata taatcaaagc caagtcgtgc
    45961 cgcattttta gcttcttcaa cggtcgcgat atcagccatg atttcaacgt tcggtgcatg
    46021 tgttctaata tatgatacta attcgtctaa cgtttctttc ggacgttgct gtaacgttgc
    46081 atccaatgca atgacttcac attggctttc tatcagttca tcaacttctt ttgacgttgc
    46141 tgtaatgaaa acatctgagt gatcatagtc acgtttcaca atgccaataa ctggtaaatc
    46201 taccgtttct ttaattgcta aaatgtcttc cttagtattt gcacgaatac caacagcacc
    46261 accttcatac gcagctaatg ccattttcga cataataaaa gatgaatgca atggttcatc
    46321 tggtagtgcc tgacaagata ctattaatcc atgtggtaac attattcctc actccataag
    46381 tcgttttttc acgtaaagtc atttacaata tatcattaag ataattttta tcaatgcttt
    46441 ttactatctt gaaaatattt ttcatatata atagtaatca ttaagatgaa ggggtgacac
    46501 aatgaaattt gagaatcgtg ttcaacgtta tcaacattta tttacaaaaa ctgataaacg
    46561 catcgtcaat tatataagac aaaatggcta tagcgatgcc ttttcaacga ttaactcctt
    46621 agcccatgcg attggtacat caccagcaac gatgacacgc tttagtcata agctggatta
    46681 tgagaatttc caagatttaa aatttaatat tcaacaagaa atgacagaga cagttattga
    46741 aaatagccca attattcaaa gaattcataa atatcatcaa caaatcattc aacaaactgg
    46801 tgaatttatt gataacgaca ttatccaaac ctttatcgac aaacttcaat caagtcgaca
    46861 tatactcttt gctggactag gtagctctgg tttatcagcg actgaatttt attatcgtat
    46921 gattcgtatg gggctaaaag gtaatgttac taccgattca catttaatga aaatatcggc
    46981 atccctacta tctcattcgg atatgtttat tgctatgtca aatagtggta atacttcgga
    47041 attaatttct gcagcggaag tcgccaaatc tcatggtgca tatgtcgttg ccatcacaaa
    47101 tttcgaaggt agtaaactta cagattgtgc agatttagta cttttaacaa cggatcaatc
    47161 gcgtaatacc gaccatcaat ttatcaacac acaaattgcg acactctttt taatcgatat
    47221 cgtgagctat catttattag aaaatacgaa tctgagtcaa acttatcaac atactaaatc
    47281 tattatccta gacaacaaat aactagtagt atattcaaat ccacctgcaa ctgtagacaa
    47341 tcatcgtgat tgtcccaacg agttgctagg tgctttttta atttcaatac caaatatttt
    47401 ttacattttc taaaatttta atatctcagc ttcttcaatt taatatgccc gtgtcacact
    47461 gttggttgat atagtttaga ttggtcatgg atataacata atctgtggaa ctaacaacag
    47521 ctctcatctc tcgtaacggt ttcaaattct attttagaat gactccttct ttcaaccatc
    47581 attgcaaaca gccatataat gctgcatcat ttttactctt agtcgtttgt attggtgcat
    47641 aaacatagtc ttttggtaaa tagtgtgcaa ctttcggctc gatatattta atgagattgt
    47701 ctccttgttc agatataccg ccaccaatta atataagccc tggatcatac atgacttgta
    47761 tttgggcaat cccttctgct acatcttctg cccactcatt caatatttgt tttgcaatat
    47821 catcaccttc ttcagctgct tcaaacaata ctggcacatg tgtgcttctc gtaaatcctc
    47881 cgtcaatcat gcgctttttc aatgcactcg ttgcagcacg ttgctcaaac gttgtatttt
    47941 cagttggacg atacaataaa tacccaactt catttgcctt atgaagttca ccattatcaa
    48001 tatggccttg attatttttg tacgcaccac caatgcctgt accaagcgtc atacaaaaga
    48061 tccgttctgc ttgatattga tgtaatttca attctcctag taatgcagcg tttacatcat
    48121 tttttacttt gacataagga gacagtgatt ttaataatcg cttaaaatta gtacctttat
    48181 aattcggaat ggttggccct gcgtatacaa tttcgccttt ttgttcatca acaacgcctg
    48241 ctgatgaaat acctatgaca ggttggatca aatgatattg cttcatatat cctgttacaa
    48301 tctcatatac tttgtcagta ataagctcac ttttgttgtc cggcgttggt atttgttgat
    48361 agtcaaacat attcaattgc ttatcaataa ctgccgattt aatttgagtg cctccaatat
    48421 cgattgcgat gtaatacacc ttactcactc catttctatt gattttgaaa aaattttcat
    48481 taattataca taaatatgac tacaattttc aaaaagatat tgttacattt tatgatagcg
    48541 tttacaatta tagctgtaaa ctatattatc aaatgatttt tggagggact tcataatgaa
    48601 caaagattta aaaggtttat atgcagcgtt actcgttcct tttgatgaaa atggtcaagt
    48661 aaatgaacaa ggacttaaac aaattgctca aaatgccatt gaaactgaag aattagacgg
    48721 tctttatgta aatggtagct caggcgaaaa ctttttatta aatacagaac agaagaagca
    48781 agttttcaaa gttgccaaag aggcagttgg ggataaagtg aaattgattg ctcaagtagg
    48841 ttcgttagat ttaaatgaag ccattgaact cggaaaatat gccacagaac tcggttatga
    48901 tgcactttct gccgtaacac cattctacta tccatttact tttgaagaaa ttagagatta
    48961 ctatttcgat attatcgaag caacacagaa caatatgatt atttatgcaa taccagattt
    49021 aacaggtgtg aatatttcta ttgaacaatt cagcgagctt tttaatcacg aaaaaattgt
    49081 tggtgttaaa tatacagcgc caaacttctt cctactcgaa cgtattagaa aagcattccc
    49141 agacaaatta atcttatctg gctttgatga aatgttagtt caagctacga tttctggcgt
    49201 agatggtgca attggttcta catataacgt taatggtcgc cgcgctagaa aaatcttcga
    49261 cttagcaaga caaggtcaaa ttcaagaagc ttaccaacta caacatgatt caaacgacat
    49321 catcgaaaca gtgttatcaa tggggattta tccaacattg aaagaaatac tacgtcaccg
    49381 tggcattgat gcaggattac cgaaacgtcc attcaaacct tttaacgaag cacatcgaca
    49441 aacattagat caactcattg caaaatacga tttatagaca tcaaagaatc aatacattgt
    49501 aaaggggatg ttgcccatga aagaagttgg gtttggcaca ctaaactggg ttgccgttat
    49561 catatatcta ctagctatgt tactcattgg cgtttatttt actaagcgcg cgagccaaag
    49621 tacgaatagt ttctttactg caagtggtcg cttgccatct tgggtagttg ggttttcaat
    49681 ttatgccact acattaagtg caattacgtt tatgtctaca ccagagaaag catttttaac
    49741 agattggtca tatattgcag gtaacatcgc tatcgtagca attattccat tactcattta
    49801 tttctatgtt ccattcttta aaaagttaaa ggtaacatct gcatatgaat atctagaagc
    49861 acgtttcgga cctagcattc gtgttattgg ctcattatta tttgtcgttt atcatctagg
    49921 gcgtgtagca atcgttatct acttaccaac attagcgatt acatctgtat cagacatgaa
    49981 cccttatatc gttgcatcac tcgttggttt actatgtatt ttatatacat ttttaggtgg
    50041 tttcgaaggt gtggtttgga gtgatttcat tcaaggcgtc attctattag gcggcgcttt
    50101 agttatcatc attcttggtg ttatgaacat taaaggcggt tttggcactg tctttgcaga
    50161 tgcgattgag cacaaaaaat taattagtgc agacaattgg aaactgaata ctgcagcagc
    50221 tgccattcca attattttcc taggaaatat tttcaacaat ttgtatcaat acacggcgag
    50281 tcaagacgtc gtgcagcgtt atcaagcttc tgatagttta aaagaaacaa ataaatcgtt
    50341 atggacaaat ggtatcctag ctttaatttc agcaccctta ttttatggta tgggtacaat
    50401 gttgtattca ttttatgcac atgaagctgt tttaccaaaa ggcttcaata catcatctgt
    50461 agtgccatat ttcattttga ctgagatgcc accatttgta gcaggattac ttattgcagc
    50521 cattttcgca gctgcacagt ctaccatttc atctagttta aattctatat ctgcttgtat
    50581 ttcaatcgac attaagcaac gcttcttcgg aaaaggtagc gaacgacacg aagttaactt
    50641 tgcacgtttc attatcatca ttgcaggtat tttcggtttt ggaatgtcac tatacttaat
    50701 tgcttctaat tcaaatgact tatgggattt attcttgttt gtaactggat tattcggcgt
    50761 tccattggct ggtgtatttg cagttggtat tttcactaaa cgtacgaata cattcggtgt
    50821 tatttgtgga ttaatattgg gtatcatctt tgcttatgtc tataatggtg ttggcaaagg
    50881 taactcacct ttctatgtat ctaccatttc atttacagtt gcttttgtct ttgcttatat
    50941 acttagcttc attgtccctt caaaacataa aaaagatata acgggattaa caatttttga
    51001 aaaagataaa ccatcaacat acatttcaaa aacggctacg aaaaagtaga ttgttatgat
    51061 aaaaccccgt cactaagtta tgatgcgctg ttgcgccacc ttggtgatgg ggtttagctt
    51121 tgccatgaat ttaatttaga tacttcgatt aatttataat actaggccaa tgattgatcc
    51181 tgaaatgatt gaagctagag ttgaaccaag taacagcctc attgcaaagg atgcaacttt
    51241 ttctccttgt ttatcactaa tgcctttaat tgaacctacg atgataccaa ccgtaccaaa
    51301 attagcgaag cttactaagt aaactgaaat gataccttgt gttcgagcag atacatcacc
    51361 caggacattt ttaaaatcaa gcattgctac aaactcattt gtaattaatt tggtcgccat
    51421 taaagagcca gctggaacag cttcgctcca tggaatcccc attaagaatg cgattggtgc
    51481 aaacacatag ccaataagct gtttaaagtt caaaccaaca ctaccaaaca tgatattaat
    51541 tgcttccatt aatgaaataa atgctaacaa cattacggct actacaacag cgattttaaa
    51601 cccatccatc gcactatcac caatcatttg gaaaaaggca actttcttag gtttccctgt
    51661 ttttccattc aatgttttag tttctgtgga tttcgttaag ttatcaattt caacatcagt
    51721 atcatcagat ttatagggat tgattacact ggcgatgata agcgcactaa aaatatttaa
    51781 cattactgct gtaactacga acttaggctc aatcatctgc atatatgaac ccaacattgt
    51841 catactaaca gcactcatac cagatgtcgc aattgtatat aattttgctc tagacaactt
    51901 tggtataata tcttttatag ttaaatacac ttctggctgt ccaaacatcg ctgtcgaaat
    51961 agcaaagtaa ctttctaaac gccccattct cgttatttta ttaatagcaa tacctacata
    52021 tttgataata aatggtaata ccttaatata attaaaaatg cctattaata cagaaatgaa
    52081 cactaatggc agcaacacat ttaaaaagaa tgtaaagcca tttttatttt gtatatctcc
    52141 aaaaacaaag tttatacctg ctttactaac atttattaac ccttcaaaaa aagaacctaa
    52201 tgcagttaag attgttaaac caatcgttgt gttcatcata aatatcacaa tcaacacttg
    52261 aataaatatc attattaacg tctttttgaa gtctatgttt ttacgatcat aactgaataa
    52321 aaacgcaaca aatagtgcaa atgctatccc tgtgatagcg aataaaatat tcatgtatca
    52381 tcacctacaa taatttatta acagcgacag caattttagc acccaacacc gcattgtttt
    52441 caacaagttt tatatttgct gctaaacttt taccattcgt tttttctaca attttcccta
    52501 acaagaacgg tgtggcgtcc ttacctttaa taccttgatt ttcggcttca acaacagctt
    52561 catttatgat tgcctcaata tatgcttttg ataaggcatg ctcatatgga attggattag
    52621 caacaacaat gccaccttca agatttaact gctgttttgt taaatgaatg tcagcaagtc
    52681 gttctggcgt ttcaaccgaa cttgttaact taacaccgct ttcgcgagtg aagaatgctg
    52741 gcaattcatt cgtttgatat ccaataactg gaacgccttt tgtttctaaa tattccatcg
    52801 tcttaggtaa gtctaaaatt gatttggcac ctgcacagat aacagtgaca tttgttttag
    52861 acaattcttc taagtctgct gaaatgtcca tcgtatgttc tgcaccttta tggacacccc
    52921 caatacctcc agtaacaaaa aattgaatac cagccattgc agcacatatc atcgtcgtcg
    52981 ctacagtagt agcaccaata cgcttcatcg caacaacttc tgctaaatcc cttctagata
    53041 ctttagcaac gtctttacta gttgccagta tttctaaatc ttcgctttct aaaccaattt
    53101 taattttgcc atctataatg gctatggttg ctggaatggc accattattc ctgataattt
    53161 gctctactgt tgttaccatt tcaacatttt gtgggtacgg cataccatgc gaaataattg
    53221 ttgattctaa tgctacaatc ggttgattgt tctcccgtgc ttgctgaact tctcgagaat
    53281 actcaatata cttttgtaaa tttgccattt ttataatcct ccatatcgtg ataaagttgc
    53341 tgttgatcta ggttttgcct aactgtatat ttcgtttcta tcgttttctt tgcattaacc
    53401 ataccagcaa ttaatatatc ttcagtagac atcccattta accagctata cactactgca
    53461 gcacagaatg aatcacctgc acctgtaaca tctttcacac tatttgatgg cataactgac
    53521 ttgatgattt cttcctcacc acttcgataa atgagttctt tcacgccatt tgtcacaata
    53581 acatttttaa cacctaaatc attccagcgt ttagcagcta tttttaaatc atcagtagat
    53641 tctattttta aatttaagta tgtttctgtt tcatctttat tcgtgataat ccaatcaata
    53701 gcatgtaatg aatcaggcat atttttcatt tttggggaag aaaccgtggt gataactaat
    53761 ttgatttgat gtttcgtggt ataggcacat aagaagttta atgcctcttt gcctaaattc
    53821 aaatcgacaa taatgcactt agcctttttc aataagtgtg aacgcttaat taaaaattca
    53881 ggcgtaatgt agtcaaacac ttccatatct gctaagccat atgtcatgtc gccttcttta
    53941 ctaattaaag ctgtatatga acctgtactc gcattttcaa attgttgaac atgatccaaa
    54001 ttcataaatg gtgtggacaa tcgtttaatc atttcccatt cactatcttg tccactagca
    54061 gataaaaaag cgaccgtttc gccaagccta cctaagttct cagcaatatt tcttgctacg
    54121 ccaccaatag agcgtgttga cgttacagga tttgatgttt ctgcaactaa atctttatgc
    54181 acataaaact tacgatctac attcgctgcg ccaatacaaa caataggata atcttcattt
    54241 aaaacatatg cctttcccat aacatattcc ttttgtatta atcctgaaat aatgtttgct
    54301 acgctgggtc tagataatcc aattgcctca gcaagttcac gttgtgaaat aaacggatta
    54361 tctttaattc tttttaaaat ttctttctca gaatcgctca taacatccct cctcatattg
    54421 acttaaaata cttgaatcag tgaatttaca aaaatatcgt agcatgcagt tatttctgtt
    54481 gtaaacgctt acatttaccg ttatatcaat gatatttgtt tataaaatac gcataatttc
    54541 gtaaaaagta agttcattcc ataaagtaaa aggcacctac gaaaatgata agtgtttatg
    54601 tggcgtgata tttgtcgact ataaattatg acatttttat gtcttatatt tttctataaa
    54661 aaaacgtgac tcattatcga ttttttcatc gactgaatca cgttactatg ttttatttgt
    54721 ttttactatt caatatgatg atgctatgcg tcaactgcac ctcgattaat catgtcttgc
    54781 ttagtaacaa ttggatattt tttaaatacg attgaaccaa gataacctgc aagcgcacct
    54841 acaatcgcca ttactacacc ataaattaca atagtcattg gatgattaaa tccaaacatt
    54901 actaaaaatc ctgcaatcgg tgtagctgta cctgtcgcat cgttaattaa acctgacata
    54961 gcaataatta aaccagcaat cgcaccacca aagaaatttg taatatagat tggaattgga
    55021 ttggctgata caatatctgc ttgtgataaa ggttcaatac ttactgcaat cgtagactta
    55081 cgatcaccta attttaagcg atggaatagc gtcccattca taaatgccga actaaatgct
    55141 gccatggcac caatagccat aggtacaccc gttaaaccta ataatgctgt caatgccatt
    55201 gaactcaatg gcgctgtgcc gacaaccgta ataataccac ctaaaatgat acccatgata
    55261 attggattcg tattcgtact actttggata atatcaccaa ttcgaatcaa tgtactattc
    55321 actactggcg ttaataatac tgcaataaga cgagctaatg gcgctaagat gacaatcgat
    55381 ccaattaagt cgacaccatc cggcacatat ttctctgtat atttcatcac atatccaatc
    55441 atataacccg caaagaaacc tggtaataaa tccataccac cgcacgcggc tgctataact
    55501 aatgcatata ctggtgacac accgatagct aatgctgtta atccagcggc agcgacacca
    55561 cctagacctc ccgcagcgtc tcctagctct tctaaaaatt taataccgaa cacttcacca
    55621 ccaacatatt tattaaatgc ttccactaaa aatgttgcga ttgctgcatt tgctaatgct
    55681 cccatggcgc gcataccatt aggtgcttta tatgtaaata atgtaaaaat cactaagacc
    55741 aaaaataaaa ataaagtacc tattaataaa tccataacca ttgaccccca ttacgataat
    55801 tttatattca atccccatat ttaaatattg aatacttaga ggccatttta ctacaaaaaa
    55861 tattatattt cacggaatat ttctaatttt cgataataat tattattaat tgaatgtgaa
    55921 agtttattca aataatacat aaacataaaa ataccggcac atgatgtacc ggcttaataa
    55981 aactattcta ctgtttcatt ttcaagatta aggacgccat ctttcatatg atatgaacga
    56041 tcacaatatg ctttaagtcg ttcatcatgt gtaacaataa tacatgcttt ctttcttttt
    56101 ttggcttgat cacgtagaat tttaatgact tcaatcgcat tttcagtatc taacgccgcg
    56161 gtaggttcat ccgctaaaat aatcgacgga ttcgtatata acgctttggc tatcgccaca
    56221 cgttgtttct gaccacctga aatttctgaa ggtaacttat taagcaatga tgttagacct
    56281 aattgtgaca taagttgctg atagtcttca ttagacataa cattcttatt tttctttttc
    56341 aataatgtaa attgttgctt taccgttaaa aatggtacaa gatttgtagc ttgtaaaata
    56401 aaacctatct cagacattct aacttttgcc aatgcttttt gcttcattgt cgtaatatct
    56461 tggttattga ttaaaatgtg cccagatgtc ggtgtttgta aagcacctgc catagttaga
    56521 aatgtacttt taccagagcc agaaggtcca actaatgcta caatatcacc tttatttatc
    56581 tcaaaatttg tatctttaac cgcttcaatg ttacgattcc catctttaaa tgactttgtt
    56641 acattttcaa atttcaacat ttgctacacc tcctacccaa tcgcctttaa cggatctatt
    56701 tttctaattg ttaaaatgga gaataaactt cctaatacag agacaatcat taacacaatt
    56761 gcaaatacga gcaatgttag tacatcgaat ttgacaggta ctgcatcagg taaaaatgca
    56821 cctgtaacgc ctgttaacag taagccaaat gccgtaccaa ataatgctaa tatgagcgtt
    56881 tgtgaaataa ctacgttcgc taaatagcca ttcgtaaatc cttgagcctt taatatgcca
    56941 aataaacttg ttttttgtaa tgtcatgaca tataagaata tacctataac tgtagctgaa
    57001 atgacaaata agaatgaaat cataaagttt aatgttaagt tctgtggttt ataacctggt
    57061 aaattttcaa taaagtcatt aatacttacc gcttcaagct cttggtttaa ttttttgtct
    57121 ttccaatttg tatcacgtac aacaactgca ttaattttat ctccagtcaa tctaggattg
    57181 attttggcaa tggtagcgtc attcgtgaaa atgactggtg acgcattata ttttgcactt
    57241 tctgtaaaac ctacgatatg caatttttca tctgattgag atagtgataa tgtgtcgcca
    57301 attttaaatc ctttattttt aagtgtttca tcagctaaca cttcattatc tttagtcgct
    57361 ttatgccctt ctattaaact cggaactaaa aatgatgact tttcaacacc gaacactaag
    57421 acattgtctt tttgatgacc attagaaact atttctcctg tttgcttcaa tgtagcttgt
    57481 ttcttgtatt tattttcaat atctttcttg ttaaaaacag attgttgcac agtttgattg
    57541 gcatctttat ttagaacaat ggcatctgct tgccacttat caatgccttc tttattcata
    57601 ttgataagac cattcgccaa tccagataat aaaaatagca agtaactaat catcgttaac
    57661 acaccaataa ttagtccaaa cttcaatttt ttgcgccgta tttcattcca agctaaaaac
    57721 atgcatttct ctccctacta ctatgattta aacattgttt atattcttag atgcacgtac
    57781 gtcgtgttgc gctctgtaat gttatacata cacttatcct tcattatacc cgaacttttt
    57841 atattaaaag caaatttatg gaaaatgcaa ttaattgtct attatttttg tacggtacat
    57901 ttaaaattaa ggatcaattt aaaaacgcct acatatacct ttaagtacat gtagacgtcc
    57961 aattcatata ttatttaatt tcgcctgttt taggatcgaa ttgcttaata gcatttttac
    58021 gtaatttatc ttttgctttg tcacttgctt tatagttatt gttgtaaatc gtagcttccc
    58081 aactaccata cattgggtta gggaaaatga tatatttctt accgaaatcg tctttatgtt
    58141 tttcaattaa tgcttcacga gattcagctg tagcttcttt tggatctgta aagtctaata
    58201 aattatctcc aaatagcatg acaagtttat gatccttttg aactatttgt ctgcgtgatt
    58261 ctttactctt atcatcttta ccttttagta aaatatgact cttcttagct tgagggatac
    58321 cttgttgttt taagttcttt tgtgttgcct ttaaatcttt ttctttatct ctatcagaaa
    58381 tatagtagat atcgacacct tttttgtcag catatttcaa gaattctttt gcgccataga
    58441 caggtttagc tttagcagct tgtacccatt catgccaacc ttctgggaaa ggtttattgt
    58501 gtattgatgc atagccttga tatggagaat tatctaaaac tgtttcatct aaatccaaag
    58561 caatagctaa cttatgttta cccttattct ttttaatctc tttatctaac tgagtctttg
    58621 cactgttata accttgtaaa tataatgctt ttgcttcagc tgaattttga taccaagcca
    58681 ctgccataat attttgatta ccaagattcg ccttttgtga tgctggtata gaagcttgtt
    58741 gtgtttgttg aacttcagca gaacttttgg caaacgctgt agaatttgtc gtttgtggtg
    58801 ccgaaactgt aaccgctacc gataatgatg ctattgcaat atactttgaa attttattca
    58861 tcttatcacc tcatgattaa tatttaaaat acagttaaaa ttataaatgc atttatttaa
    58921 tattgctata ctatgaaaag atatttaata ttatttcttg aaaagctaac aaatatgtga
    58981 acatttcata taagcatgat ttaatgggag acctatcaca tgagagagat ttgtgattta
    59041 tcacgtaata aactttaatt catttgagtg actaggcctt cctatcagtc atattcactc
    59101 atccacgtat cattatgtgt acagtgtgct atctcttgtt tacctattgg aacaaccata
    59161 aactcatcca tagtttacct tttataaata gcagtcctca ctcatacaat ttctcataaa
    59221 aatcacaacg ctccaacgta tttccaactt actttcacct attttaattc ataaaaacga
    59281 cactttaatt gtcattatcc aataatagca agacgttatt attgcaatct tttttataaa
    59341 ataatagaat catagtattg tcatttaaag ataaagtaag aacgttttta tttttcagat
    59401 tttttaaatt attatgaata tctagtttta ggaaggaaat tacattgaaa aaacaagtta
    59461 ttatttcggg cctcatgtta ttttcactat tttttggagc cggaaattta atattcccgc
    59521 ccatgctcgg ccatacagcg ggtcaaaata tgtggattgg tatgctaggc tttgctctta
    59581 caggcatatt actccccttt attactgtta ttgttgttgc attttatgat gaaggtgttg
    59641 aaagtgtagg caatcgtata catccatggt tcgggtttat ttttgctgtc gtgatttaca
    59701 tgtctatcgg agcattttac ggtattccac gtgctgcaaa tgtcgcgtac gaaattggta
    59761 caagacacat tttacctgtg cacaaccaat ggactttaat tatattcgca gcaatctttt
    59821 ttgctatcgt ttactggatt agtttaaatc catcgaaaat cgttgataat ttaggtaaat
    59881 tattaacacc gttattacta ttaatggtcg ctctattaag tattgctgtc attttcaacc
    59941 ctgaatctgt actaagtgca cctaaggata aatatataac acatcctttc atttcaggaa
    60001 gtttggaagg ctattttaca atggatcttg ttgctgcgtt agctttttcc gtagtcattg
    60061 tcaatggcta taagtttaaa ggcctcacag atcgcatgaa aattttaaaa tatgtctgct
    60121 tttcaggtct tattgcagcc atattacttg gaatgattta ctttgcactt gcatacgttg
    60181 gggcatcaac agctccagga aactttaaag atggtacaga tatattgacg tacaactcat
    60241 tacgagtatt tggttcgttc ggtaacctcg tatttggaat gacggttatt cttgcatgcc
    60301 taacaacatg tataggactc gtcaacgctt gtgcaacatt tactaagaaa catgtaccta
    60361 agttttctta taaaatattc gcacttgttt tctccatcat aggattttta tttacaacac
    60421 ttggtttaga aatgatttta aaaattgctg taccattgtt gacattaata tatcccgtgt
    60481 ctattgcact tgtactgata tcatttgcta acatgtttag cacattcaga ttcagttggg
    60541 cctatcgact cgcaactgtt attacattga ttatttcaat tttacaaata ctaaatagtt
    60601 tcaacttatt acacggtgtt attttgaaat ggtttatgat gttaccttta gcagatatcg
    60661 atttagcttg gcttgtacca ttcatgctct ttgctattat cggtttcatc atcgatgtat
    60721 ttatacgtcg tccgaaacaa gcgacaactt aataaatgct cactgcctag taatgattga
    60781 cccatcgtta ctaggctttt ttatatgaaa ttcatgactt ttcagtgata ccgtctgtct
    60841 attaattatt ttaaaataaa tgatttgtta aaaaatccta gctgttattc aaaaatatta
    60901 gtttttaaaa ttaattttgt gaaattaatt ttgtgaaatt attcacaaat aaggcgattc
    60961 atagtatact ctgattaaca aatgaaaagc gaggatgtca ttgtgaagga gaaacatatt
    61021 aaatgggata aaatatttta tggggacgac tgggtaaata acgtagtcga aacgataaga
    61081 acgaaagata tattacaaag cgtttattta aaacgttatc tattacgcgc gatgatggca
    61141 ggatttatta tcgggattat tacggtcttc gtattatcag ttaaagcaac acacgaacca
    61201 gatttaccgc caggcattgt gaatatggcc agcgccatta cattcagttt tgcgttagta
    61261 ctcattttat ttacaaactc cgaactacta accagtaact tcatgtactt tactgtaggc
    61321 ctgtattata aagtaattaa accgactaga gtattgaaaa tatttttatt gtgctttgca
    61381 ggaaatattt taggtgctgc tattttattt agtttcatgc gtttttcaaa tgtaatgacg
    61441 ccagatatgt taaatcaatt atcagcagtt atagaacata aaacgttgtc tactgggttt
    61501 gtaagtatat taatgaaagc gatattcgcc aactttttta ttaatatttc attagttatc
    61561 gccatgcaaa ttgacgatgt acttgcaaaa atgtttgtca tgatgttcgg tgttaccata
    61621 ttcgccttta tgggttatga acatgtcgtt tacaacagtt gcttgtttat gggaggctta
    61681 atttatcaag ttgatacatt gcacttcatt cctgctattt caaatattgc tgccgctttt
    61741 atcggtaact atatcggagg cggtctcatt ataggtttat tctatgctta cttgaatgat
    61801 catcatcaat tttataaaaa taattaactc tatttgaaca atgcgttagt cgatgccttt
    61861 aatatttaga agtgcattgt ctaacgtatt tttttaaagt tcgtcaatgg tttttgtccg
    61921 atgctatgag cattatcaaa tacaaaatgc tctcttaaaa gcagttattg actgaaaatc
    61981 tacttctaag agagcaattt atttaattgg ttaatttatt aaaaactaac agaaatataa
    62041 ttacttaaga aatcttgaaa tttcaatata cgatgtttat gataagtcgc ttatttcatc
    62101 tttaggcttg ttattagtaa ggagtttaat accactgact aaccataaag caaatgtaat
    62161 tatgttacca cttattacag ctccaataat caacaatatt ccactcattt ttttgttttt
    62221 agatgcttta aacataccga ttgcacctaa aataattgaa atgattccaa atatgaatag
    62281 gaataagaat aatacagtga aaattgctgc tgctgtttct gcatcaactg ggtcaacctc
    62341 accattaact gttgttgaca catctgtagt cattacacct gatgctgcta aaaccatcaa
    62401 tagtaaacta aataccgttg ctaaaccttg aacaacaatt cctattattg tcattatttt
    62461 gatcgatttt tccattgcct tactccttta ttatta
//
LOCUS       DATTQF010000002        62212 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.002, whole genome shotgun sequence.
ACCESSION   DATTQF010000002 DATTQF010000000
VERSION     DATTQF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 62212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.002"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..319
                     /locus_tag="VB241_000058"
     CDS             <1..319
                     /locus_tag="VB241_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1243397.1"
                     /translation="KEKQKSIYYKEQQARITLYLKHNTIEPNTIKSVHFTNLETSPMG
                     SAVIEGYINENKEDDFVAYASPENNFQFVGDLIKSERLSELLKPAHQLKSPDDIKKEL
                     NKK"
     gene            346..772
                     /locus_tag="VB241_000059"
                     /pseudo
     CDS             346..772
                     /locus_tag="VB241_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
     gene            1024..1443
                     /locus_tag="VB241_000060"
     CDS             1024..1443
                     /locus_tag="VB241_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1243398.1"
                     /translation="MSKKKILILTNIMLIILISVAGIYFKMKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTIEPNTIKSVHFTNLETSPMGSAMIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAQKLKSPDDIKKELNKKKGH"
     gene            1461..1874
                     /locus_tag="VB241_000061"
     CDS             1461..1874
                     /locus_tag="VB241_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1243399.1"
                     /translation="MSKKHVFIIIGVILCICIVATVIHFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDIILSKNLSEIIKIKTKSPDEIKEELDKKEGR"
     gene            2327..2749
                     /locus_tag="VB241_000062"
     CDS             2327..2749
                     /locus_tag="VB241_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEP1243400.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(2861..4399)
                     /locus_tag="VB241_000063"
     CDS             complement(2861..4399)
                     /locus_tag="VB241_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEP1243401.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAMMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     VSVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIAWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            4634..5452
                     /locus_tag="VB241_000064"
     CDS             4634..5452
                     /locus_tag="VB241_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1243402.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            5553..6320
                     /locus_tag="VB241_000065"
     CDS             5553..6320
                     /locus_tag="VB241_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1243403.1"
                     /translation="MNIRHIRNATAVIEYGGKRVLIDPMLSDKGAFEPFPNSPRQDEN
                     NPLVELPLAIEDIINNIDLVILTHLHIDHFDPKAIEVLPKDIKIYTQNEADASEVEGY
                     DFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGLVLKHSEEPTLYLAADTV
                     WFDGVEKTLKAHHPDIIVLNGGANQFFEGGPLVMDEHDVLKVAHTLSLAQIVVVNMEA
                     VNHWHLSREELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            6895..7716
                     /gene="cntK"
                     /locus_tag="VB241_000066"
     CDS             6895..7716
                     /gene="cntK"
                     /locus_tag="VB241_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HEP1243404.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVSQVTTE
                     IQHLVETYVREQQWNHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            7727..8545
                     /gene="cntL"
                     /locus_tag="VB241_000067"
     CDS             7727..8545
                     /gene="cntL"
                     /locus_tag="VB241_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HEP1243405.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEVLETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            8538..9839
                     /gene="cntM"
                     /locus_tag="VB241_000068"
     CDS             8538..9839
                     /gene="cntM"
                     /locus_tag="VB241_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HEP1243406.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGEMLGIKTPMINQFLTRYEA
                     SCKTYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            9982..11580
                     /gene="cntA"
                     /locus_tag="VB241_000069"
     CDS             9982..11580
                     /gene="cntA"
                     /locus_tag="VB241_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HEP1243407.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            11593..12528
                     /locus_tag="VB241_000070"
     CDS             11593..12528
                     /locus_tag="VB241_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243408.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTMISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            12525..13394
                     /locus_tag="VB241_000071"
     CDS             12525..13394
                     /locus_tag="VB241_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243409.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            13391..14206
                     /locus_tag="VB241_000072"
     CDS             13391..14206
                     /locus_tag="VB241_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243410.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIVGLNPERLRVTGDITFDGKSMLSLSETQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            14199..14948
                     /locus_tag="VB241_000073"
     CDS             14199..14948
                     /locus_tag="VB241_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243411.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            14960..16153
                     /locus_tag="VB241_000074"
     CDS             14960..16153
                     /locus_tag="VB241_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1243412.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PANSL"
     gene            complement(16377..17126)
                     /locus_tag="VB241_000075"
     CDS             complement(16377..17126)
                     /locus_tag="VB241_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1243413.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRMQIVASA
                     KYKWHILISFTLSIVLFLLLNHFAIFALNVDSFTVFDIYPLIREYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            17322..18899
                     /locus_tag="VB241_000076"
     CDS             17322..18899
                     /locus_tag="VB241_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEP1243414.1"
                     /translation="MTFLTVMQFIVNIIIIGFLLTVMVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLTNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(19067..19522)
                     /locus_tag="VB241_000077"
     CDS             complement(19067..19522)
                     /locus_tag="VB241_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HEP1243415.1"
                     /translation="MKKLCSLIVVALVCVIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            19760..20836
                     /locus_tag="VB241_000078"
     CDS             19760..20836
                     /locus_tag="VB241_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEP1243416.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLTNDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKKHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSVRLVTEIVRSLNDETYKNIMW"
     gene            21182..21844
                     /locus_tag="VB241_000079"
     CDS             21182..21844
                     /locus_tag="VB241_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1243417.1"
                     /translation="MLLEIKDLVYEASDRVILNHINLKVDKGETIAIIGPSGSGKSTF
                     QKLICNLISPTSGTLYFKGKPYDDYEPEILRRHISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDINNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            21837..22613
                     /gene="fetB"
                     /locus_tag="VB241_000080"
     CDS             21837..22613
                     /gene="fetB"
                     /locus_tag="VB241_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEP1243418.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFLISFVAIFVGTALP
                     LVGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TTKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            23007..24194
                     /locus_tag="VB241_000081"
     CDS             23007..24194
                     /locus_tag="VB241_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1243419.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGSLGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(24256..25608)
                     /locus_tag="VB241_000082"
     CDS             complement(24256..25608)
                     /locus_tag="VB241_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEP1243420.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSATFTEHEDCLYLNIWKQHNDQSKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSIWTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(25915..27552)
                     /locus_tag="VB241_000083"
     CDS             complement(25915..27552)
                     /locus_tag="VB241_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP1243421.1"
                     /translation="MIKDKQLHNEKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMKHLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSY"
     gene            27733..28332
                     /locus_tag="VB241_000084"
     CDS             27733..28332
                     /locus_tag="VB241_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HEP1243422.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLEE
                     IQARREEIKYYIAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            28367..28476
                     /locus_tag="VB241_000085"
                     /pseudo
     CDS             28367..28476
                     /locus_tag="VB241_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28556..28639
                     /locus_tag="VB241_000086"
     CDS             28556..28639
                     /locus_tag="VB241_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243423.1"
                     /translation="MGVCPPVTMDACALLQKFDFCNNISHF"
     gene            29002..30228
                     /locus_tag="VB241_000087"
     CDS             29002..30228
                     /locus_tag="VB241_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243424.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMTHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDRDNHLLGFLDIEDINQGIRGHKSLRETMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEYVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            30225..30860
                     /locus_tag="VB241_000088"
     CDS             30225..30860
                     /locus_tag="VB241_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243425.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLILGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            30877..31818
                     /locus_tag="VB241_000089"
     CDS             30877..31818
                     /locus_tag="VB241_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1243426.1"
                     /translation="MKKIKYILVVLVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGV
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFNSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            31818..32513
                     /locus_tag="VB241_000090"
     CDS             31818..32513
                     /locus_tag="VB241_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243427.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            32807..34207
                     /locus_tag="VB241_000091"
     CDS             32807..34207
                     /locus_tag="VB241_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1243428.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLVA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            34433..35368
                     /locus_tag="VB241_000092"
     CDS             34433..35368
                     /locus_tag="VB241_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP1243429.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKPHIDNETIVVC
                     TMNGLKHEEIIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            35690..36541
                     /locus_tag="VB241_000093"
     CDS             35690..36541
                     /locus_tag="VB241_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP1243430.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(36648..38147)
                     /locus_tag="VB241_000094"
     CDS             complement(36648..38147)
                     /locus_tag="VB241_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP1243431.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQNNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLTLTTAFIALFSMLITLLLILHTTTIKKP"
     gene            38379..40208
                     /locus_tag="VB241_000095"
     CDS             38379..40208
                     /locus_tag="VB241_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP1243432.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFDDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVHNVEVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(40348..42426)
                     /locus_tag="VB241_000096"
     CDS             complement(40348..42426)
                     /locus_tag="VB241_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEP1243433.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAIEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITQG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKD"
     gene            complement(42564..43973)
                     /locus_tag="VB241_000097"
     CDS             complement(42564..43973)
                     /locus_tag="VB241_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1243434.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            44259..45065
                     /locus_tag="VB241_000098"
     CDS             44259..45065
                     /locus_tag="VB241_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1243435.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKNLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQRGKHYGATWQDKERISWGTSDQNLSKIA
                     AEQVKKDLLEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(45128..45784)
                     /locus_tag="VB241_000099"
     CDS             complement(45128..45784)
                     /locus_tag="VB241_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEP1243436.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPDAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQKQNKHT
                     "
     gene            45897..46370
                     /locus_tag="VB241_000100"
     CDS             45897..46370
                     /locus_tag="VB241_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1243437.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYTIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(46493..47704)
                     /locus_tag="VB241_000101"
     CDS             complement(46493..47704)
                     /locus_tag="VB241_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEP1243438.1"
                     /translation="MQSSKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILASGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTPKEELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(47886..48539)
                     /locus_tag="VB241_000102"
     CDS             complement(47886..48539)
                     /locus_tag="VB241_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243439.1"
                     /translation="MKRTDKYRDSYQYDNQNHRRQSEDASYRQQYAKGDPEEHPERYY
                     NGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDPKV
                     SQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADSKL
                     TQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGAN"
     gene            complement(48599..49741)
                     /locus_tag="VB241_000103"
     CDS             complement(48599..49741)
                     /locus_tag="VB241_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP1243440.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGKTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGDNLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            50009..50395
                     /gene="gtxA"
                     /locus_tag="VB241_000104"
     CDS             50009..50395
                     /gene="gtxA"
                     /locus_tag="VB241_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HEP1243441.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNISLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            50529..50636
                     /locus_tag="VB241_000105"
     CDS             50529..50636
                     /locus_tag="VB241_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEP1243442.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            51284..53047
                     /locus_tag="VB241_000106"
     CDS             51284..53047
                     /locus_tag="VB241_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243443.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIVLIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFIAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGIDIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            53072..54805
                     /locus_tag="VB241_000107"
     CDS             53072..54805
                     /locus_tag="VB241_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243444.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLVAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNGSKRINTKIEMQFIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGDPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            55036..55192
                     /locus_tag="VB241_000108"
                     /pseudo
     CDS             55036..55192
                     /locus_tag="VB241_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            55247..55933
                     /gene="bioD"
                     /locus_tag="VB241_000109"
     CDS             55247..55933
                     /gene="bioD"
                     /locus_tag="VB241_000109"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEP1243445.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMANQIFLNKQCVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTGSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            55911..57269
                     /gene="bioA"
                     /locus_tag="VB241_000110"
     CDS             55911..57269
                     /gene="bioA"
                     /locus_tag="VB241_000110"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEP1243446.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKCKLLSQIETTSSTLEKQLHALKRHRNVGYVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            57271..58278
                     /gene="bioB"
                     /locus_tag="VB241_000111"
     CDS             57271..58278
                     /gene="bioB"
                     /locus_tag="VB241_000111"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEP1243447.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSREIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            58256..59371
                     /locus_tag="VB241_000112"
     CDS             58256..59371
                     /locus_tag="VB241_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEP1243448.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFESFMRKYSYHLSSSRLISGSSTVYEEIESMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAE
                     YPNQNIQKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGI
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            59382..60074
                     /locus_tag="VB241_000113"
     CDS             59382..60074
                     /locus_tag="VB241_000113"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HEP1243449.1"
                     /translation="MYSIKMRSSNQDIHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIKQIKALQIIEDDKANLQHLTQKCGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGHRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYILFL
                     ESTPKQVVYETV"
     gene            60085..60552
                     /locus_tag="VB241_000114"
     CDS             60085..60552
                     /locus_tag="VB241_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP1243450.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLILSVILNLPVWVVLLNAIPGVIFTLIVAIPLYLILRKRMAVLLR"
     gene            complement(60604..61581)
                     /gene="hlgB"
                     /locus_tag="VB241_000115"
     CDS             complement(60604..61581)
                     /gene="hlgB"
                     /locus_tag="VB241_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HEP1243451.1"
                     /translation="MNMNKLVKSSVATSMALLLLSNTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKAAGNINSGYERPNPKDYDFSKI
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(61583..>62212)
                     /gene="hlgC"
                     /locus_tag="VB241_000116"
     CDS             complement(61583..>62212)
                     /gene="hlgC"
                     /locus_tag="VB241_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HEP1243452.1"
                     /translation="FQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQS
                     APSLGGNGSFNYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDL
                     FVGYKPHSKDPRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMD
                     VTHAIKRSTHYGNSYLDGHRVHNAFKNRNYTVKYEVNWKTHEIKVKGQN"
BASE COUNT        21247 a         9093 c        11729 g        20143 t
ORIGIN      
        1 aaaagaaaaa caaaaatcaa tttattataa agaacaacaa gcgcgcatta cactttatct
       61 taagcataat actatagaac cgaacacaat caaatctgta catttcacaa acttggaaac
      121 aagtcctatg ggaagtgctg tgattgaagg ttacataaat gaaaataaag aagatgattt
      181 tgttgcctat gcatcacctg aaaataactt tcaatttgta ggtgatttaa ttaaaagtga
      241 aagattaagt gagttactaa aaccagcgca tcaattaaaa tcaccagatg atataaaaaa
      301 agaactaaat aaaaagtaga gtcactaaag ttaggagtta ctttaatgaa caaaaaacat
      361 gtttttataa ttattggtgt cattttatgt atatgtatag ttgcatcagt catttattta
      421 aaaatgaaat tatgacgaaa aagaaaaaca aaaagcactt tactacaaag gacaacaaga
      481 gcgcattaca ctttatctca agcataatac caaagaaccc aataccatca aatctgtgca
      541 tttcacaagt ttaaagacag gacctatggg cgatgcagta attgaaggat acatcaatga
      601 acacaaaaaa gctgattttg ttgcatatgg gtcacctgaa gaaaactatc aattcactgg
      661 aggattgaca ggaagtaatg aagtcttagg aaaattgaag aacgctgaaa atttaaaatc
      721 tcctgaaaaa attaaagaag aaataaacaa aaagaaaaac actaaacagt gattttcacg
      781 taatatttag cttgatttgt acatgtttaa tatcgattgc tatcgaagat atttatgtta
      841 gtagggtgtc gaattttata gaagcggaat atcatatcgt tcaattctca gtaatagtta
      901 attctaatat tcacgttgtt ggatacgacg atagtggtac tatgatttat aaactataac
      961 attaattatt acagaactat aaaaattttt cattttccac cttacattag gagctggtga
     1021 tacatgtcga aaaagaaaat tttaatatta actaatatta tgttaatcat attaatcagt
     1081 gttgcaggta tctattttaa aatgaaatat gacgaaaaag aaaaacaaaa atcaatttat
     1141 tataaagaac aacaagagcg cattacactt tatctcaagc ataatactat agaaccgaac
     1201 acgatcaaat ctgtacattt cactaacttg gaaacaagtc ctatgggaag tgctatgatt
     1261 gaaggttaca taaatgaaaa caaaaaagat aattttactg cttatgctac accagaacat
     1321 aattatcaat ttggtggcgc tatgatagaa agtgaaagat taagtgaatt actaaaacca
     1381 gcacagaaat taaaatcgcc tgatgatata aaaaaagagc taaacaaaaa gaagggtcac
     1441 taaaagttgg aggtggttaa atgtccaaaa aacatgtttt tataattatt ggtgttattt
     1501 tatgtatatg tatagtcgct acggtcatac attttaaaat gaaatatgat gaaaaagaaa
     1561 agcaaaaagc aatttattat aaagaacaac aagcgcgtat tacactttat ctcaagcata
     1621 acactaaaga accgaatacg atcaaatctg tacacttcac aaagttggaa acaagtccta
     1681 tgggaagtgc agtgattgaa ggatacataa acgaaaacaa aaaagatgat tttgttgctt
     1741 atgcatcgcc tgaaaataac tttcaatttg taggtgatat aatactaagc aaaaatttat
     1801 cggaaataat taaaataaaa acaaaatcac ctgacgaaat caaagaagaa ttagacaaaa
     1861 aagaaggccg ctagggtctt ctttattttt gatttaatct tccaataatc tatttcattg
     1921 ctatcgaagg tgtttcgcaa ttaatataaa tcacttcatc atcaccaata cttcgccagt
     1981 ttttttacag tacattaaca caagtgaacc ctattaatat aaatgaaata atagtttggc
     2041 cattataaaa acattatata ccgaataaca atggcgatga cgtgatttat tgacattatc
     2101 aaattttctc aaatgtgtgt gattttttat aaaaaagggt agagcaaggt ataatatcct
     2161 ttcaatctgt tttcatattt tatatatttt ttaaatataa gcgctaaatg ttttaactaa
     2221 agcacagatt gacaagatgt tatacagaat ttcaaattct atccaatatt gttcgaagtg
     2281 tagtatcact ggattggtat taaacaatgt aaaggagaga ttgcaaatgc cgtataatta
     2341 caagaaacaa aatggagagt taatgtctgt aatgagccaa ggtgaaaagt ttattcatca
     2401 atcacccgtt aatgatgaac ttagtgcatt gattaagtta ttaatttcaa aaattaacgg
     2461 ttgtcattat tgtgttgata tccataaaaa agaattaaag gaattgggtg taacacaaat
     2521 gaaaattgat gaagtcttga gttttagaca tttagattta tttactgatc aagaaaaagt
     2581 gacgcttgaa tttgcagaaa tgttaaattc aatcaaagac tttaagaagt ttgaaattat
     2641 tgaccggcta aaatcatttt atgatgaaga acaaattatt gatcttgtct ttgttgtaaa
     2701 ccaaattaac ggttggaaca gactaaatat tattagtgat aaactataat tgttcatata
     2761 aatacagagt ttcatctcga acgctatatc ataacaaatc atgccactat acaggtcaaa
     2821 tcttgtatag tggcatttta aattatcccc ttgagtgctg ttatttaacg aatatcggtc
     2881 cacctggtcc aacagggata cctaataaga accaaataat gacaaatact gtccacgcaa
     2941 tacttagcgc gattgaatac ggcattaaac tagaaagtaa ggctccgagt ttcatgcgtt
     3001 tatcgtattt ttgtgcataa gttaatagta aaggtaagta cggcatcatc ggtgtaattg
     3061 gattggtaat tgaatctcct acacggtaaa tgacttgtgt aaatgcgggg tgaaagccga
     3121 taaggattaa cattggtacg aatatcggtc ctaaaatacc ccatttagcc gatgcgcttc
     3181 cgattaacat gttgaccatt gcactcagta caataatacc tagtatcaat acaataccgt
     3241 tttgatgttc taataatttg gcacctttaa cagcagcgat aattcctaaa ttactccact
     3301 ttaaatacgc aagtagctgt gctgcaaaaa acacaataac gataaatgtt cccattgatc
     3361 ctacagcatc gccaaacatt ttacctaagt cttttgtatt tttaatttct ttgcttaaaa
     3421 tcccataaac taatccaggt actaaaaata caacaagaat aattaatccg acaccgttaa
     3481 ttaatggcgc atcgtctagt aagctgcctg ttttagcatt tcttaaaaag ctatgttcag
     3541 gaatggctgt aataattaat aaaataattg tgactatgaa actgatattt gcccatttta
     3601 aagcatgtgc ttctttgtca gtaatatgtg aagacgtttc ttcatgatca tcatgcatta
     3661 aacgatcatc atattgacct aacctaggaa taatcagttt agttgtaact agcaatatcg
     3721 taggaagtaa tacaacgaca ctcactgcga taaagtacca gttcatagca acatttgttt
     3781 taatagaatc tgaaacgata cgtgttgctg gttctgtaaa tgaatagact aaagcatctt
     3841 gcataccaac aactatattt gcggcaaagc cacctacagc ggaagcatat gccatcgtta
     3901 gtccagcgat agggtgatag ccaattttaa taaaaagcat cgctgcgagc ggcggcaaga
     3961 taattgtagc agcatcgccg gctgtactac ctaaaatccc aattaatata atagtcggta
     4021 aaattaagaa acgtggtgcg cgattcacaa cagaaatcat taacttgtcg aagtatcctg
     4081 ttttctctgc aacacctata ccaatcatca ttgctagtac taagcctaat gctgggaact
     4141 ctgagaaatt tttaatcgta tcattcatta tcattgtaaa tccatcatgg ctaattatat
     4201 ttttaatata aatggtttga tgcgtacctg gatgcttaac agatacatta aataatgaga
     4261 taacccacgt cataatggct aagcccacac acattaaaaa gaataaaacg ctaggatctg
     4321 gcaatttatt tccaattttt tcaacactat tcaagaaacg attgacgata gacccctttt
     4381 gttgatgttt tgatgtcatc aatgattccc ccctttgtta aatatttaaa gtgtaacaaa
     4441 aaatactctc aaaagtaaca attttcagaa aatgaaaaat ctaatattgt taaatatttt
     4501 gagttattca atagaaattg tatagcagag agtagctaag actgcctgga gacttagcta
     4561 ttaggtttat gaagcattga acagtggaaa ataaggactg taagtttaag atatgttgta
     4621 taggagtgac tgaatgaaac gtttggaaaa taaagttgca gtcgtaacag gagcaagtac
     4681 aggtatcgga caagcttctg caatcgcttt agctcaagaa ggtgcgtatg tattggcggt
     4741 agacatagct gaagcggtat cggagactgt cgataaaatt aaaagtaatg gtgacaaagc
     4801 aaaggcttat attgtggata ttgcaagcga acaacaaatt gacaattttg catcagaaat
     4861 tagagaacag attgggcatg ttgatgtgtt atttaataat gccggtgtgg ataatgcggc
     4921 tggtagaatt catgagtatc caacagatgt atatgacaag attatgaatg tagatatgcg
     4981 tggaacattt ttgatgacga aaatgatgtt acctttaatg atgactaaag gtggctctat
     5041 tgttaacaca tcttcatttt caggacaggc agcagatttg tatcgctcag gatataatgc
     5101 tgcgaaaggg gcagtgatta attttactaa atccattgca atcgaatatg gtagagatgg
     5161 cattcgagct aatgcaattg caccaggtac aattgaaacc ccgttagtag ataaactgac
     5221 aggtactagc gaagataagg aaggcaaagc gtttagagaa aatcagaaat ggatgacacc
     5281 gctgggacgt ttaggcaaac cagaagaagt aggcaagctc gttgtctttt tagcatctga
     5341 tgagagttca tttattactg gtgaaacaat tcgcattgac ggtggtgtta tggcttacac
     5401 atggcctggt gagatgttga gtgatgattc gtggaagcgg acattggaat aattgtttta
     5461 aaatcgatat ccagtggaaa ataatttggc atgaagtagg caatgtatgc atattgtcgc
     5521 aatgacagac atatgaaata gggggattta acatgaatat tagacatata agaaatgcca
     5581 cggccgtgat tgagtatgga ggtaaacgcg ttttgattga tccaatgcta agtgacaaag
     5641 gcgcatttga accatttcca aattcaccga gacaagatga aaacaatcca cttgtagaac
     5701 taccattagc aattgaagat atcattaaca acatcgattt agtaatttta acacatttgc
     5761 atattgatca ttttgatccg aaagcgattg aagtattgcc aaaagatatt aaaatttata
     5821 cacaaaacga agcagacgct agtgaagtgg agggttatga ctttacaaac gtttcggtat
     5881 ttaatgatgt gacacacatt ggagaaattg agttgattaa aacagatgct caacatgggc
     5941 atggtgaaat cttgaaaatg acaggacatg ttcacggttt agtgctgaaa cattctgaag
     6001 agccaacgtt atatttagct gctgatacgg tttggttcga cggtgttgag aagactttaa
     6061 aagcgcatca tccagatatc atagtactta atggtggtgc aaatcaattc tttgaaggcg
     6121 ggccgttagt gatggatgaa catgatgtat tgaaagtcgc tcatacattg tcgcttgctc
     6181 aaatagttgt tgtgaatatg gaagcggtca atcattggca tttatctcgt gaagagctga
     6241 atgaatttat aaattctaac gacttaggta atagagttgt tgtaccaaat gacggggaat
     6301 tattaacctt tgaaaaataa atatttgttt atttgatgaa atgtattgct taaagctagt
     6361 tttaacgtat tttacgttga agctggcttt tttgtttgat aaaaattttc aaaaagactt
     6421 gaatgtgctg agaatgcgat gtttatggag ggattcataa aatataaatg tgctttcatt
     6481 tacaaccttt attgacaatg attctcaact aatatagtat ataatcaaat cgtaatagtt
     6541 acgatttgtt ttctgcaact tttttgaagt tttagttgag gtgaaaacag taaaagcatc
     6601 taagtgaatg tagttaacgg acaactgcat tcgcttgtag agccacaaga agcaacttta
     6661 aataaggttt acggttgcat gttgatgcaa caaccgatta ctaagtcatg ctttccactt
     6721 tgcggattag catgacttac ctaatagatg ggaaactatt aggttcatct tctgaaaaat
     6781 cacttaaggt aaaagagcta gaggaataca gttgcttgct tcgcaacaac tgcataagag
     6841 cctctagtaa ttaaaattac agaggctcta aaaatacatc taaaggagtg tcgtatgaat
     6901 cggcaggtta tagaattttc taagtataat ccttcgggga atatgacgat acttgttcat
     6961 tcaaaacatg atgctagtga atatgcatct atcgccaatc agttgatggc cgcaacacat
     7021 gtatgttgtg aacaggtagg ctttatagaa tcaacacaaa atgatgatgg caatgatttt
     7081 cacttagtta tgagcggtaa tgaattttgc ggtaatgcga cgatgtcata tatacatcat
     7141 ttgcaggaaa gtcatttgct taaagaccaa cagtttaagg tgaaggtatc tggctgttcg
     7201 gatttagtgc aatgcgcaat tcatgactgc caatactatg aagttcaaat gccacaagcc
     7261 catcgtgttg tgccaacaac aattaatatg ggtaatcatt catggaaagc aatagaaatt
     7321 atttatgaaa catatgtaca ttatgtgatt ccagttagtc aagtaacaac agaaattcaa
     7381 catttggttg aaacatatgt gcgtgagcaa caatggaatc acaaatataa aacagtaggt
     7441 atgatgcttt ttgatgaaca acgtcaattt ttacagccat taatctatat accagaaatc
     7501 caaagtttaa tttgggaaaa tagctgtggt tctggtacag catcaattgg ggtttttaat
     7561 aattatcaac gtaatgacgc atgcaaagat tttacagtac atcagccagg gggcagtatt
     7621 ttagtgacat caaagcgatg tcatcaattg ggatatcaaa cttcaattaa aggacaggtt
     7681 acaactgtag ctacaggaaa agcatatata gaataaggag cctacaatga ataactttaa
     7741 taatgaaatc aaattgatat tacaacaata tttagaaaag tttgaagcgc attacgagcg
     7801 tgtattacaa gacgatcaat atatcgaagt attagaaaca ttgatggatg actatagtga
     7861 atttatttta aatcctattt atgaacaaca atttaatgct tggcgtgacg ttgaagaaaa
     7921 agcacaatta ataaaatcac tgcaatatat tacagcgcag tgtgttaaac aagtggaagt
     7981 cattagagct agacgtctat tagacggaca ggcgtctacc acaggttact ttgacaatat
     8041 agaacattgt attgatgaag agtttggaca atgtagtata gctagcaatg acaaattatt
     8101 gttagttggt tcaggtgcat atccaatgac gttaattcaa gtagcaaaag aaacaggtgc
     8161 ttcagttatc ggtattgata ttgatccaca agccgttgac ctaggacgca gaatcgttaa
     8221 cgtcttagca ccaaatgaag atataacaat tacggatcaa aaggtatctg aacttaaaga
     8281 tatcaaagat gtgacgcata tcatattcag ctcgacaatt cctttaaagt acagcatttt
     8341 agaagaatta tatgatttaa caaatgaaaa tgtcgtagtt gcaatgcgct ttggtgatgg
     8401 catcaaagca atatttaatt atccgtcaca agaaacagcg gaagataagt ggcaatgtgt
     8461 gaataaacat atgagaccac agcaaatttt tgatatagca ctttataaaa aagcagctat
     8521 aaaggtaggt attacggatg tctaaattat taatgatagg cactggtccg gtcgcaatcc
     8581 aattagcgaa tatttgctat ttaaaatcag attatgagat tgatatggtt ggacgtgcct
     8641 caacatcaga aaaatcaaaa cgcttatatc aagcgtataa aaaagagaaa caatttgaag
     8701 tcaaaataca aaacgaggcg catcaacatc tggaaggtaa gtttgaaatt aatcgtttgt
     8761 ataaagatgt taaaaacgtt aagggtgaat acgaaacggt tgtcatggca tgcacagcag
     8821 atgcttatta tgacacacta cagcaattgt cgttagaaac tttgcaaagt gtcaaacatg
     8881 tcattttaat ttcaccgaca tttggatcgc aaatgattgt ggaacaattt atgtctaagt
     8941 tcagtcagga tatcgaagtg atttcatttt caacttatct tggcgataca cgtattgttg
     9001 ataaagaagc acctaatcat gtgttgacaa caggtgtaaa aaagaaattg tacatgggat
     9061 cgacacattc aaactcaaca atgtgtcaac gaatctctgc tttagctgag cagttgaaga
     9121 ttcaattaga agtcgttgaa tcgccactgc atgctgaaac gcgaaacagc tcgctttatg
     9181 tgcacccacc actatttatg aatgactttt cattgaaagc cattttcgaa ggaacggatg
     9241 taccggttta tgtgtataag ttatttcctg aaggaccgat aacgatgaca ctaatccgtg
     9301 aaatgcgttt aatgtggaag gaaatgatgg ctattttaca agcatttaga gtaccgtcag
     9361 tcaacctgct tcaatttatg gtgaaggaaa attatccagt acgtcctgaa actttggatg
     9421 aaggtgatat tgagcatttc gaaatcttgc cagatatctt acaagaatat ctgctttatg
     9481 taagatatac cgcaatcctt attgatccat tttcacagcc agacgaaaac ggacattact
     9541 ttgatttttc agctgtacca tttaagcaag tctataaaaa tgaacaggat gttgttcaaa
     9601 ttccaagaat gccaagtgag gattattaca gaacagcgat gattcagcat attggggaaa
     9661 tgctaggtat caaaacgcca atgattaatc agttcctaac tcgctatgaa gcaagttgta
     9721 agacgtacaa ggatatgcat caagatcaac aactatcttc tcaatttaat acaaatctat
     9781 ttgaaggaga taaggcactc gtcacaaaat ttttggaaat caatagaacg ctttcataat
     9841 aagggtttga agttttataa tagaaaaaaa ttattgaatt atgtttgaca tttacataaa
     9901 aataagcaaa taattgagaa aaataatcat tacgatttga ttaagtaatg caacttatca
     9961 atttagaaag aggaaaagca aatgagaaaa ctaactaaaa tgagtgcaat gttacttgca
    10021 tcagggctaa ttttaactgg ttgtggcggt aataaaggtt tagaggagaa aaaagaaaac
    10081 aagcaattaa cgtatacgac ggttaaagat atcggggata tgaatccgca tgtttacggt
    10141 ggatcgatgt ctgctgaaag tatgatatac gagccgcttg tacgtaacac gaaagatggg
    10201 attaagcctt tactagctaa aaaatgggat gtgtcagaag atgggaagac atacacgttc
    10261 catttgagag atgacgttaa attccatgat ggtacgccat ttgatgctga cgcagttaag
    10321 aaaaatattg acgcagttca agaaaacaaa aaattgcatt cttggttaaa gatttcaaca
    10381 ttaattgaca atgttaaagt taaagataag tacacggttg aattaaattt gaaagaagca
    10441 tatcaacctg cattggctga attagcgatg cctcgtccat atgtatttgt atctccaaaa
    10501 gactttaaaa acggcacaac aaaagatggc gttaaaaagt tcgatggtac tggtccattt
    10561 aaattaggtg aacacaaaaa agatgagtct gcagacttta ataaaaatga tcaatactgg
    10621 ggcgaaaagt ctaaacttaa caaagtacaa gcaaaagtaa tgcctgctgg tgaaacagca
    10681 ttcctatcaa tgaaaaaagg tgaaacaaac tttgccttca cagacgatag aggtacagat
    10741 agcttagaca aagactcttt aaaacaattg aaagatacag gtgactatca agttaagcgt
    10801 agtcaaccta tgaatacgaa aatgttagtg gtcaattctg gtaaaaaaga taacgctgtc
    10861 agtgacaaaa cagtcagaca agcgattggt catatggtaa acagagataa aattgccaaa
    10921 gaaattttag atggtcaaga aaaaccagca actcaattat ttgcgaaaaa tgtaacagac
    10981 attaatttcg atatgccaac acgtaagtat gaccttaaaa aagcagaatc attattagat
    11041 gaagctggtt ggaagaaagg taaagacagc gatgttcgtc aaaaagatgg taaaaacctt
    11101 gaaatggcaa tgtactatga caaaggttct tcaagtcaaa aagaacaagc agaatactta
    11161 caagcagaat ttaagaaaat gggtattaag ttaaacatca atggcgaaac atcagataaa
    11221 attgctgaac gtcgtacttc tggtgattat gacttaatgt tcaaccaaac ttggggatta
    11281 ttgtacgatc cacaaagtac tattgcagca tttaaagcga aaaatggtta tgaaagtgca
    11341 acatcaggca ttgagaacaa agataaaata tacaacagca ttgatgacgc atttaaaatc
    11401 caaaacggta aagagcgttc agacgcttat aaaaacattt tgaaacaaat tgatgatgaa
    11461 ggtattttta tccctatttc acacggtagt atgacagttg ttgcgccgaa agatttagaa
    11521 aaagtatcat tcacacaatc acagtatgaa ttaccattca atgaaatgca gtataaataa
    11581 aggagcgttt agatgttcaa atttatctta aaacgtattg cgctcatgtt tccattgatg
    11641 attgtagtaa gttttatgac atttctattg acgtatatta caaatgaaaa tccagctgtg
    11701 acaattttac atgcacaagg aacgccaaat gtaacaccag agttgattgc agaaacgaat
    11761 gagaagtacg gtttcaatga tccattatta attcaatata aaaattggtt acttgaagcg
    11821 atgcaattta attttggtac aagctacatt acaggtgatc ctgttgctga acgcattggt
    11881 ccagcattta tgaatacatt gaaattaaca atgatttcaa gtgttatggt gatgattaca
    11941 tcaattattt taggtgtagt tagtgcatta aaaagaggaa agttcactga tcgtgcgata
    12001 cgttcagtgg ctttctttct aactgcatta ccatcatatt ggatagcttc aatacttatt
    12061 atttacgttt cagtgaagtt aaacatattg ccgacttctg gattaacagg tccagaaagt
    12121 tacatattgc cagtgatcgt tattacgatt gcctatgctg gtatttactt tagaaatgtt
    12181 agacgctcga tggtggaaca attaaatgaa gattatgtac tttatttaag agcaagcggt
    12241 gtgaaatcta tcacattaat gttgcatgtg ttgcgtaatg ctttacaagt tgcggtatca
    12301 atcttttgta tgtctatacc aatgataatg ggtggactag ttgttatcga gtatatcttt
    12361 gcatggcctg gattaggtca attaagttta aaagcaatac ttgaacatga ttttccagtc
    12421 attcaagcat atgtattaat tgtagcggta ttatttattg tatttaatac attagcagat
    12481 atcattaatg cgctattaaa tccaagatta agggagggcg cacgatgata attttaaaac
    12541 gattattaca agataaaggt gcagtgattg ctttaggcat tattgtatta tatgtctttt
    12601 taggattagc agcaccactt gtgacatttt atgatcctaa ccatatcgat acagcaaaca
    12661 aatttgctgg catgagtttt caacatctac taggtactga ccatttaggt agagatattt
    12721 taactaggtt aatttatgcg attagaccaa gtttgttata tgtctttgtt gcactatttg
    12781 tttctgtact tattggatct attttaggat tcttatcagg atatttccaa gggtttgttg
    12841 acgccttaat catgcgtgcg tgtgatgtta tgttggcatt cccaagttat gttgtaacgt
    12901 tagcattaat tgcattgttt ggtatgggtg ccgaaaatat tatcatggca tttattttga
    12961 cgcgttgggc atggttctgt cgcgttatac gtacaagtgt tatgcagtac actgcttctg
    13021 accatgtaag atttgctaaa acaatcggta tgaatgatat gaaaattatt cacaaacata
    13081 ttatgccgtt aacattagca gatattgcta tcatctctag tagttcgatg tgttcaatga
    13141 tcttgcaaat atctggcttt tcatttttag gattaggtgt caaagcgcct actgcagagt
    13201 ggggcatgat gcttaacgaa gctagaaaag tgatgtttac acatcctgaa atgatgtttg
    13261 cgccaggtat tgccatagtg attatagtca tggcatttaa cttcttatcc gatgctttac
    13321 aaattgccat tgatccccgc atctcttcta aagataaact tcgttctgtg aaaaaaggag
    13381 tggtgcaatc atgacattgt taacagttaa gcatttgacg attacagata cctggacaga
    13441 tcaaccactc gtgagtgatg tgaattttac attaactaag ggtgaaactt taggcgttat
    13501 tggagaaagt ggtagcggta aatcaattac atgcaaatcg attgtaggtt taaatccaga
    13561 acgattacgc gtaacaggtg atataacctt tgatggtaag tcaatgttgt cgttatcgga
    13621 aacacaattg aaaaaatacc gtggtaaaga tattgcgatg gtcatgcaac aaggtagtcg
    13681 tgcctttgat ccatcaacta ctgtcggtaa acaaatgttt gagactatga aagtacatac
    13741 gtcaatgtct acacaagaaa ttgaaaagac attgattgaa tatatggatt atttaagttt
    13801 gaaagatcct aaacgtatat taaaatcata cccttacatg ttatcaggag gaatgttaca
    13861 gcgattgatg attgctttag cgttagcttt gaaaccaaag ttaatcattg ctgatgagcc
    13921 gacaacggct ttagatacaa ttacacaata tgatgtactg gaagcattta tagatataaa
    13981 aaaacacttt gactgtgcga tgattttcat ttcacatgat ttaacggtta ttaacaagat
    14041 tgcagaccgt gttgttgtga tgaaaaatgg tcaactgatt gagcatggta cgcgtgaatc
    14101 agtcttgcat catccagaac atgtttatac gaagtattta ttatccacta agaaaaagat
    14161 taatgatcat tttaaacatg tgatgagggg tgatgtacat gattaaaatt aaagatgttg
    14221 aaaagtcata tcaaagcgca catgttttta agcgtcgtcg aacacctatc gtgaaaggtg
    14281 tgtcatttga gtgtccaatc ggtgcgacga ttgcgattat cggagaaagt ggtagcggta
    14341 aatcgacgtt gagtcgtatg atattaggta ttgagaaacc agataaaggt tgtgtaacct
    14401 taaatgatca accgatgcat aagaagaaag tgagacgtca tcaaattggt gctgtatttc
    14461 aagattatac gtcatcatta cacccatttc agactgttag agaaatctta tttgaagtga
    14521 tgtgtcaatg tgatggacaa cctaaagatg ttatggaagt ccaagcaatt acattgttgg
    14581 aagaagttgg tctatctaag gcatacatgg ataaatatcc taatatgtta tcaggtggag
    14641 aggcacagcg tgttgcgatt gcgcgcgcaa tatgtattaa ccctaaatat attttgtttg
    14701 atgaagccat tagttcactc gacatgtcaa ttcaaacaca aatattagat ttattgattc
    14761 atttacgtga aacgcgtcag ttgagttata tttttatcac acatgatatt caagctgcca
    14821 cgtatttatg tgatcaatta attattttta aaaacggaaa aatagaagaa caaattccga
    14881 caagcgcatt gcataaaagt gacaatgctt atacaagaga attaatagaa aaacaactat
    14941 cattctaagg agtgagataa tgaaaggtgc aatggcttgg ccctttttga gattatatat
    15001 attaacattg atgttcttta gtgccaatgc aatcttaaac gtgtttatac ctttacgagg
    15061 gcatgattta ggcgcaacga atacggttat cggtatcgtt atgggggcat acatgttaac
    15121 agcaatggta tttcgaccat gggcaggaca aattattgct cgtgtcggtc ccattaaagt
    15181 attaagaatt attttgatta tcaatgccat agctttaatt atttatggtt ttactggctt
    15241 agaaggttat ttcgtagcac gtgttatgca aggtgtgtgt acggcattct tttctatgtc
    15301 tttacagcta ggtattattg atgcattacc ggaggaacat cgttctgaag gtgtatcatt
    15361 gtactcgcta ttttcaacga ttccaaactt aatcggacca ttagttgccg taggtatttg
    15421 gaatgcaaat aatatttcac tatttgcaat tgtcattatc tttatcgcat taacaacaac
    15481 attctttggc tatcgcgtga cctttgctga acaggaaccc gatacgtcag ataagattga
    15541 aaaaatgccg tttaacgctg taactgtttt tgcgcaattt ttcaaaaata aagagttgtt
    15601 gaacagtggt attatcatga ttgttgcatc gattgtattt ggtgcagtta gtacatttgt
    15661 accgttatac acagtgagtt taggattcgc gaatgcggga atctttttga caatacaggc
    15721 catcgcagtt gttgtggcaa gattttactt aaggaaatac attccgtcag atggtatgtg
    15781 gcatcctaaa tatatggtat ctgtactatc attattagta atcgcgtcat ttgtagtagc
    15841 atttggtccg caagtaggtg caattatttt ctatggcagt gcaatattaa taggaatgac
    15901 acaagcaatg gtgtacccaa cattaacatc atacttaagt ttcgtcttac caaaagtagg
    15961 tcgtaatatg ttgttaggtt tatttattgc ctgtgcagac ttaggtatat cgttaggtgg
    16021 cgcattgatg ggacctattt ccgatttagt aggatttaaa tggatgtatc taatttgtgg
    16081 tatgttagtc attgtaataa tgattatgag tttattgaaa aaaccaacac cacgcccagc
    16141 aaatagtctt taatgaagtg aattaaagca tattaagtta atgaatattt aaattttaaa
    16201 aggtatattg agcatggcga ttcatgtgct tcatgctagg acatgaaaca ttctatatgg
    16261 ctcgttttta gaacgacagt atatctaaat aaagcacgct taaaagtgag ttgtgatact
    16321 gacttttaag tgtgcttttt gatgtgtatg gataatggat aatactctga aaaaatttac
    16381 atgaaaatat gcaaaacgag tataactgct aattgataga aatagctcac cataaaatta
    16441 cggtttgatt ttaaatataa gtaagtcgca ctactagcta gtatcaatgc tggaatgaat
    16501 tcccaccatg tattaatgta tggataatag tacagtgttt caaggataac ggataatact
    16561 attgtaatct ttaaaggtat taatctgctt aattcttgaa ttaaaatatg acggaaaata
    16621 agttgacaaa tcaaagtatt taatataatg gttaacgaaa atatagctat taaactgatg
    16681 gaaccatatt ctctaatgag cgggtaaatg tcaaagacag taaaggaatc tacatttagt
    16741 gcgaaaatag cgaaatgatt taaaagtaaa aagagtacga tacttaatgt aaatgatata
    16801 agaatatgcc atttatattt agcactggca acgatttgca tacgtatcat tggaacaaat
    16861 gcatcttcat gcatccgacg aaaaatagct agtgaaataa taactgcgag taaatagcta
    16921 atgttcattg aaataggaaa agagaaaccc catggggctt gttgagtgaa tacagctact
    16981 aacccaaaag ttaaaaaaac gataatgatt ggcaagatgt taaccaaaaa tatataaagg
    17041 aaaataaatc caatatcacg tttgaaaaaa cgcgattgtt cggtagcgta ttcttcttct
    17101 atgtaatgtt tatttgtatt tgacatagta tacctcttaa atagttgtat tatatagata
    17161 ctttagcaca tattactttg tattgtatgt tttatacatt aaaatttaaa atgaaaaaca
    17221 tatcataaaa ttgttttata aaatgaagcg cttccattgt gttttatttt gtaaggtgta
    17281 tcataaatat tgaattgaaa ttttttgggg gagggattgt aatgacgttt cttacagtca
    17341 tgcaatttat agttaatatt atcattatag gcttcctgct tacggtcatg gttatcgggc
    17401 ttatttggtt aattaaagat aaaagacaat cacaacatag tgtattaagg aattatcctt
    17461 tactagcacg tattagatat atttcagaaa aaatgggacc ggaattacgt cagtatttat
    17521 tttctgggga taatgaaggg aaaccttttt cacgtaatga ttataaaaat atcgttttgg
    17581 ctggaaaata taactctcgt atgaccagct tcggtactac taaagattat caagacggct
    17641 tttacataca gaacacaatg tttccgatgc aacgtaatga gatttcagta gataatacaa
    17701 cattgttatc aacattcatt tataaaatcg cgaatgagcg tttatttagt cgtgaagaat
    17761 atcgtgtacc gacaaagatt gatccgtatt acttaagtga tgatcatgca ataaaattag
    17821 gtgaacattt aaaacatccg tttattttaa aacgtatcgt aggacaatcg ggtatgagtt
    17881 atggagcctt aggaaaaaat gccattacag ctttatctaa aggtctagct aaagcgggca
    17941 cttggatgaa tacaggtgaa ggtggcttat cagaatatca tttaaaaggt aatggggata
    18001 taattttcca aattggtccc ggtttatttg gtgttcgtga taaggaaggt aattttagtg
    18061 aaggtttatt taaagaggtc gcacagttaa ctaacgttcg cgcatttgag ctgaagttgg
    18121 cacaaggtgc taagactcgt ggtggtcata tggaagctga aaaggtaaac gaagaaattg
    18181 ctaaaatccg aaatgttgaa ccttataaaa caatcaattc acctaaccgt tacgaattta
    18241 ttcataatgc tgaagatttg attcgtttcg tcgatcagtt gcagcaatta ggtcaaaaac
    18301 cagtaggatt caaaattgta gtaagcaaag tttcagaaat tgaaacactt gtacgtacga
    18361 tggtggaatt agataagtat ccaagcttta ttacgattga cggtggtgaa ggtggtactg
    18421 gtgcaacatt ccaagaatta caagatggtg ttggcttacc gctatttaca gctctaccta
    18481 ttgtgtctgg catgttagaa aaatatggta ttcgagataa agtgaaatta gcggcatctg
    18541 gtaagttagt gacaccagat aaaattgcga ttgcactagg tttaggtgca gattttgtta
    18601 atattgcacg tggcatgatg attagtgtcg gttgtattat gagtcaacaa tgtcatatga
    18661 atacatgtcc tgtaggtgtt gcaacgacag atgcgaaaaa agaaaaagca ttgattgttg
    18721 gagaaaagca atatcgtgtc acaaactatg taacaagttt gcatgaaggc ttattcaata
    18781 ttgcagcagc tgttggcgta tcaagcccta cagaaattac tgctgatcat attgtatatc
    18841 gaaaagtcga tggtgaattg caaacgatac atgattataa attaaaactc attagttaac
    18901 ttatttattt cgggaaaatg aaagcagcgg attttagcgt tactgcaaat aattttatat
    18961 tagtagtgga tgttggtcac acaagaattt caaatattaa agccctcaga atatgaatta
    19021 aggtttgtaa ccttagtctt atctgagggc atttttatgt tataaactat ttgtcgtcca
    19081 ttttatcttt ttcttttaag cctctgtgct ttaattgctt ttcaagtttt tcaaaactaa
    19141 tatctttatt ttctttagtt gaaacaccaa gacgtttatt taattttttc atgtcaactt
    19201 ctgtgtaatc tatgtctaag tgttcaattg cttttttatc tttatagtct actttgtatt
    19261 ttacgccttt aaggtctttg aaaatacttt cagatttggc gaataacttt ttggcttcgt
    19321 ctttatccat acctagatcg tcatatttaa ttgtgttgat tgtagactgt tttaaaactt
    19381 tatcatcttt atatgtgata gaagttagta catgtttacc actaacatca ccttcatatg
    19441 ttttggtttg ttctttacca caagctgata atgcaatgac acaaactaat gctactacaa
    19501 ttaatgaaca taattttttc aaagtcagtc gccttctttc gatatttgta ttataaagaa
    19561 attataacat ttactaaaaa atgatgttat tcaaaaattt aaattttgat aatttttttg
    19621 taaatatgag gtttttgaag cggatttctc acaaagtttt taaaattatt aagcctgaaa
    19681 acgataaagc ggtagggaac gtttttctga aagttagtga tacaatagtt ttaagttgaa
    19741 atacaggagg atgaataaca tgaatcagtc agtcaaatta cttaaacatt taacagatgt
    19801 aaacggcatt gctggttatg aaatgcaagt taaagaagca atgcgtaact atatagagcc
    19861 tgtcagtgat caaattattg aagataattt gggtggcatt tttggaaaga aaaatgctga
    19921 gaatggtcaa tactcaatta tgatttctgg ccatatggat gaagttggtt ttatggtaac
    19981 aaagattgat aaacatggtt ttatttcatt tacgccagtt ggtggatggt ggaatcaagt
    20041 catgttatct caaaaagtaa cgattacaac agattcaggc aaagaaatta gaggtatcat
    20101 cggttctaaa ccgccgcatg tcttaacgcc tgaagaacgt aaaaagccaa tggaaatcaa
    20161 aaatatgttt atagatattg gtgttagtag caaggaagaa gctgaagaag ctggcgttga
    20221 agtaggcaat atggttacgc catatagtga atttgaagtg cttacaaatg ataaatattt
    20281 aactgcgaaa gcatttgata atcgctatgg ctgtgcatta gctgttgagg tattaaaacg
    20341 tttaaaagat gaaaatattg gcattaactt atacagtggt gccacagtgc aagaagaagt
    20401 tgggttacgt ggtgcgaaag tggcagcgaa tacaattaaa ccagatttgg cgatagccgt
    20461 cgatgtgggt attgcttatg atacaccagg tatgtcaggt caaacgagtg atagtaaact
    20521 aggcggtggt ccagttgtta ttatgatgga tgctacaagt attgcgcacc aaggtttgcg
    20581 aaagcatatt aaagatgtag ctaagaaaca taacatcgaa gtacaatggg atacgacacc
    20641 aggtggaggt acagatgcgg gaagtattca tgtcgcaaat gaaggtattc caacgatgac
    20701 aataggtgtc acgttacgat acatgcactc taatgtatcg gtactcaatg tagatgatta
    20761 tgaaaattct gtccgtcttg ttactgaaat tgtccgttca ttgaatgatg aaacttataa
    20821 aaatatcatg tggtagtcaa atccataaat aataaagaaa gtctttaata tgatgtcatg
    20881 caagaactaa gttagatgct tagttagcat gagatgtcat gttaaaggct ttttattttt
    20941 ataaagatga gactaagtca ctgcagtgta cttcaatttt cacataaata ttaaaaaaat
    21001 tttccagaat gaaatggcac taaatttttc aaaaatggga atactatatt tgatatgttt
    21061 tattagagcg attttaatgg gtaataagca ttgactaggc cgacttatag gtaagcacgc
    21121 tattcaagaa aaggattacc ctattatatc gaaataactc atgatacgag gaggggtaaa
    21181 tatgttgtta gagataaaag atttagtgta tgaggcaagt gatagagtca tactaaatca
    21241 tattaattta aaagtagata aaggtgaaac cattgcaatt attggtcctt caggtagtgg
    21301 taaaagtaca tttcaaaaat taatatgtaa tttaattagc ccaaccagtg gaacgcttta
    21361 ctttaaaggt aaaccatatg atgattacga accagaaata ttacgccgac acattagcta
    21421 tttaatgcaa caaagcgatt tgtttggtga aacgattgaa gacaatatga tttttccgtc
    21481 tcttgcgcgt aatgataaat ttgataaaaa acgtgctaag caattaatta aagatgtagg
    21541 attaggacac tatcaattaa gttctaaaat agaacatatg tcaggtggcg agcgtcaaag
    21601 gatcgcgatt gcacgccaat taatgtatac cccagacatc cttctattag atgaatcaac
    21661 aagtgcgtta gatattaata ataaagaaaa aatagaaaat atcattttta aattagcaga
    21721 tcaaggcgtg gcaattatgt ggattaccca cagcgatgac caaagtatgc gacactttca
    21781 aaagcgtata accattgttg atggtcaaat ttctaataca gaggagttga accaacatga
    21841 gtaatacagc attaggactg acggcattgc tattagttat tccaattatt atttcttata
    21901 aagaaggtct acatattatt aaagatttga ttgtggcgac actacgtgcg gtcgtacaat
    21961 taatcatttt aggattctta ctgcactata tttttaaaat taatgacaaa tggctattag
    22021 tattatgtgt atttgtcatt attgtcaacg catcatggaa tacgattagt cgatcatcac
    22081 ctgtcatgca ccatgtcttt ttgatttcct ttgtggctat ctttgttgga acggcattac
    22141 cgcttgtagg tactattgcg acaggggcca ttcaatttac agccaatgaa gttataccta
    22201 tcggcggcat gcttgcaaat aatggcttga ttgcaattaa tttagcttac caaaatttag
    22261 atcgtgcatt cgtacaagat ggtactaata ttgaatctaa attatcactt gcagctacaa
    22321 ctaaattggc ttctaaaggt gcaatacgtg aaagtattcg tttagctata gtgccaacaa
    22381 ttgattcggt taaaacatat gggcttgtgt cgattcctgg tatgatgaca ggcttaatta
    22441 ttggtggcgt accaccttta caagcgatta aatttcaatt gttagtcgtg tttattcata
    22501 caactgcgac cattatgtct gctttgattg cgacatattt aagctatggt caatttttca
    22561 atgcaagaca tcaattagta gcacgaaata cggatgttaa gagtgagtca tgatagcaat
    22621 tgctggatgt gatttagagc ttcgctatag ttgtaatgaa gtgacgcgca catatagtaa
    22681 tgttattatt tagcgcagcg aaaatattca taaaggcacg catactttgt agtcagttat
    22741 atgttctgac atataaagcg tgcgtgcttt tttggagtca ttgttgaaac tgaagtaatt
    22801 atacataatt attaaatgac atacttgtgt taatttttca aatactgaaa aacaatttca
    22861 ataattttcc aattaagcac agaaaattaa agcaaaatat tatataatag aaaggttata
    22921 tataaaattg tgttgcacac attttttaat aaatcgttat tctaagggaa atgaatatcg
    22981 gaaattttgt tgaaaggagt tttaaaatgt caatcatgcg actatttaca ttcattttaa
    23041 gtatttttat cgtaggaatg gttgaaatga tggttgcagg aattatgaac ttgatgagtc
    23101 aggacttaca tgtatcagaa gctgtcgttg gtcaattagt gacaatgtac gctttaacat
    23161 ttgcgatatg tggacctatt cttgttaaat taacgaaccg tttttcatca aggcctgtat
    23221 tattatggac attacttata tttatcattg gtaatggcat tattgctgta gcgccaaatt
    23281 tttcaatatt agtagttggt agaattatct catctgcagc agcagcacta attatcgtaa
    23341 aagtattagc tattacagcg atgttatcag cacctaaaaa tcgtggcaaa atgattggac
    23401 ttgtctatac agggtttagt ggtgccaatg tttttggtgt accaattgga acggttatcg
    23461 gcgatttagt aggttggcgc tatacatttc tattcttaat tattgtgagt attattgttg
    23521 gcttcttaat gatgatctat ttaccgaagg atcaggaaat acaacgaggc cctgtgaatc
    23581 atgaggcacc atctcatgaa aatcatgtta cttcgaaaat attaagacct gctgaagtag
    23641 caaaatattt aattattacg tttttagtat tgattgcaaa ctcagtgaca ttcgtcttta
    23701 taaatccact tattttatct aatggtcatg atatgtcatt cgtttcatta gcacttctag
    23761 taaatggaat cgctggcgtt attggaacat cattaggtgg tatattctct gataaaatta
    23821 caagtaagcg ttggttaatg atttctgttt ctatttttat cgtgatgatg ttacttatga
    23881 atttgatctt acctggttca ggtctattgt tagcaggact atttatttgg aatatcatgc
    23941 aatggagtac taatccagca gtgcaaagcg gtgtgattca acatgttgaa ggcgacacaa
    24001 gccaagtaat gagttggaac atgtctagtt taaacgctgg tattggtgtt ggaggcatta
    24061 ttggaggctt ggtcatgaca catgtttctg ttcaagctat cacgtatacg agtgccatca
    24121 ttggctcatt aggattaatc gttgttttca cattgaaaaa taatcattat gctaaaacat
    24181 ttaaatcatc ataattctca tatgaaaagc acgcctgcta tcaaattcag gtgtgctttt
    24241 ttagatgcga taacgttatt gatatgtgcg ataatagcga cgttcattat gatacatcgg
    24301 ccaaggcatt ttaccgcttt tagcaaaatt agctaaatca ttttgcattt gtcgacttaa
    24361 aaatttaagg tgagcagttg ttggatattg atgtgctgct aatatttgta agtttccgaa
    24421 ccaaaatacc atatctagaa tatggtaagc actgcgatag tgtgcactag aagtatcatg
    24481 ccatgcaaat tcagcaagcc atagttgtgc gtttgaatct tgtatattga gttgttgtaa
    24541 aaattgcttc atcggctgtt tgaaataaca atgtgtaatc gctaaagatt gttgcttcgc
    24601 cgtctgaaca tcttcgtatt ttaaaggaat atcatttaat tccataatgt cgataaagcg
    24661 ttgcggcgat aatttttttt gttcactagt aatataaata tcgccttcat cttttgtata
    24721 acatgcaaaa attggtttcg ttgttggata attattttgt atataatctg ttttaatagg
    24781 cgcatatatt aaatcaagcc ctttagaagg tcctcgagat tgttttagct tcgccatcag
    24841 cataagaata tcatctgtcg ataatgacgt aacatcatct gtatctaaat aatcgagcat
    24901 caatttttgg aaatgttgtg ctttattgcg tgcactctca agggtgtcta atcgtagtgc
    24961 gccacttagc aaaacgactt tatggaagta tggctcaatg tcaggtattt taagtaaagt
    25021 ccaaatgctc atactgcctg cagactgacc cattaaagta atgttattag cgtcgccacc
    25081 gaaggattca ataaattgat gcacccattt tatgacattg atttgatctg aaagtccatt
    25141 attggaatga aaacctttat taaaatatga ccagtctaaa tatcctaatg cgcctaaacg
    25201 ataattgcat gtaataacga taatgtcgtt attttgtact aaatgtgccg gttgatagag
    25261 ttcggctgta ccatgaccat tttcaaaact accaccataa aaataaatga tgacaggttt
    25321 cttcgactga tcattatgtt gtttccaaat atttaaatat aaacagtctt catgttcagt
    25381 aaaagttgca gattgtgagg agaaaaaatc ttctaatttg ttgtctggtt gcggtgggat
    25441 gggttgtatt tcagttgcat caattggctc tgaccattgt gtttttaacg tagaatgttt
    25501 aaagcgattg tcatgaactg gtggttctgc ataaggaatg cctaagaaga tatctaaacc
    25561 atcttgtgta ataccatgaa tttgaccacc tgtagtatta atcttcatat ttattaacct
    25621 cctttatcat ccccaattta atcataccac ggtctatgga tgataggtga caagtataac
    25681 aagacaattt tgtggctgaa tcagacgagc ttgatatctg ttttaactca aaccattttc
    25741 agcattatat gatgttcttc tacagtgaca tttcattaca atatcactct cgctatcttg
    25801 ttatgttagt tatttataac ccattttcaa aataaaaaac caaccaagat gttgaaatca
    25861 atggttggtt aatgtgtgtg ttgaaatcat atatggcact gcattttaaa atatttaata
    25921 agatgacttt gattcattat ttagtgactc gcgcatcttt gtattgattc gtgttgcgcg
    25981 acggaaatag acgctttcaa atgaactcgt tgtaccgatg taatagaaga taagcgccac
    26041 aataataata acgataaagt caaatggata gtgaatcata tttaagcctt taaattcttt
    26101 gcttcctata aatgacagta ttgatagcac gattaaatat acaataatcc ataagctacc
    26161 accaatttgt ttctttgtat tacgccaatt catacgatat tcatagaaga agtagattgg
    26221 taagccaagt ataatgatta aaattacttc agccgttgtt ggccacattg cccaatatat
    26281 agctaatgaa gctaatacaa atgataatgg tgccattact tttaaaattt ttgctctaaa
    26341 tggacgagtc attgttggtc ccatttttct taatgcaatc actgtcgttg ggccagttaa
    26401 ataggctact aaagttgcag tagaaataac tgctgctagc gtaccccaat ctctaaataa
    26461 cgtaaccata atcatactaa tgatggcatt aaagatgatt gctacacgtg gaatatgata
    26521 tttttcgttc atcttcccaa gaaatttagg gatatgtcca tttttctcca ttgctcgtaa
    26581 aactcgacct gtaacggcga caaatgagac gccagtaccg aatggtgata caaaagcttc
    26641 aatatatagt aaaattgcga gccaattaat tcctaataag atagctaaat cagcaaatgg
    26701 tgaattgaag ttgatgccat tccatccact atgttgtaac attgattgag gcatagaagt
    26761 gataaacgta ctttgtaaaa tgatgtacaa cactgcactg attgacagtg aaatagcgat
    26821 gcctcttgcg atattttttt caggattttt aatttctgaa cccatattaa taattgtctg
    26881 gaatgaattg aatgaaaaaa taatccctga tgctgttgtt gcagcaaaaa tcggtgcact
    26941 tccgtaaggc ataaatgtgc ttgctgaatg tccgtaattt gaagtgtcga atcctgataa
    27001 cattaacatg atgatggtta acatgggtac gcctaattta aatacagaaa ttaaactcgt
    27061 aaatgatgtt aaaagtttta cggaccaata gtttaataaa gaaaaaataa caatgatgag
    27121 atatacagct agcaatccgt atgtgctaat agagccattt tccattaaat gtttcattgg
    27181 tttcgcccaa tgccacggcc aagaactcat atattgaaca gctgacacag cttcgatagg
    27241 tattattgtc accaaagaca cccaattcgc ccaagcagca ataaagccta ataatgagcc
    27301 atgtgtatac tgggcatagt tactcatgcc acctgattga ggaaacattg tgccgatttc
    27361 aatgtagtta taggcaatgg ttccaatgac taggaatcca agaacccatg atatgattgc
    27421 tgctggtcca gctattgatg atgcttccca cgcaccaaat agccaaccag atcctattaa
    27481 agagcctaac cctaataaga ctaattggga tagattgatt ttttcgttgt gcaattgctt
    27541 gtctttaatc ataaatactc cttcgttatt gccgaaaatt caaaaaatag tatgcgaaaa
    27601 attatacgct tattataata atagtcaaat gcattttttg taagaatgca aaagcatttg
    27661 taaaattagt tttttataaa attaggatat ataatacaat gtgtattgaa aaataaaatg
    27721 gaagtgatga ctatgagtga gaaacaatcg aatgaacaag ttgaggcatt tttaaataaa
    27781 gagagtcaat ggcaagaaga atataaatat ttgagagcgt taatctttaa tgaaacagaa
    27841 ttagaggaag cgtataaatg gatgcatcct tgttacacgt tgaataataa aaatgtagta
    27901 cttatccatg gcttcaaaaa ttatgttgca ctattatttc ataaaggtgc cattttggag
    27961 gataaatatc atacactcat tcaacagact gaaaaggtgc aagcagctcg tcagttacga
    28021 tttgaaaatc tagaagagat tcaagcacgt cgagaagaaa ttaaatacta tattgctgaa
    28081 gcaattaaag ctgaaaaagc tggtaaaaaa gttgaaatga agaaaacaga ggaatatgtt
    28141 attcctaaag agttagaagc taagttcgaa gaaatgccac agctagagtc gtcattttat
    28201 aaattgacac caggcagaca gcatcaatac atttatcaca ttggacaagc taaacgcagt
    28261 gaaacaagac aaaagcgtgt tgaaaagtat attaaccaaa tactagaagg taaagggatg
    28321 catgataagt aattaatgag taaaagcata ccggttatac aacaacatac aagatgacac
    28381 gaaacaacca atggctcatg ctgttggttg ttttttaggt gtgcctatca tgggcaacac
    28441 tttgacggtg gaattccgtt acaggcttgg gagtaggaac tgttagcata aggcaagggt
    28501 gtctacaatg aatgatgaag atattgaaat ataaggagga ctttgtgagt ggcgggtggg
    28561 cgtttgtccg cctgtaacaa tggatgcgtg tgcattatta caaaaattcg acttttgcaa
    28621 taatatttca catttttgac acttttttgc tataaaacaa ccaattgagc gataataaat
    28681 tcgcttttga aaaatatgag ttatctattt agttgcaaaa gataaaatga taatgtttaa
    28741 taacatcata tagagtatgt tagttttaaa tgtcgaatat acgaatgtgc aaacaaagta
    28801 atcggtagaa attcaacata catagcgccg tttactgtta agtattcaca ttacagatga
    28861 aaaatataaa attctacata atcaagacca tgatgtgtac ttgtttaact tatgactcta
    28921 tttgtttaac aattgcgatg atggtctttt tattttatgc gtatcattcg tcatattttt
    28981 tatgaggaag gagaaatgat tatgttaagt attaagcatt taacgaaaat ctattctggt
    29041 aataaaaagg cagtagatga catctcttta gatattcagt ctggggaatt tatcgcattt
    29101 attggaacca gtggaagtgg caaaacgact gctttaagaa tgataaaccg tatgattgaa
    29161 gcgacagaag gacaaattga aattgatggt aaagacgttc ggagtatgaa tcctgtcgaa
    29221 ttacgtagaa atattggcta tgttattcaa caaattggct taatgactca tatgacgatt
    29281 aaagagaata ttgtgttggt acccaaattg ttgaaatgga ctaaagaaga aaaggataaa
    29341 cgtgcaaagg aattaattaa acttgtggat ttaccggagt catttttaga gcgttatcca
    29401 gcagaactat caggtgggca acaacaacgt atcggtgtcg taagagcact tgcggccgaa
    29461 caagatatta ttttaatgga tgaacctttt ggagcattgg atcctattac gagagatacg
    29521 ttacaagatt tagttaaaac gttacaacga aaattaggca agacgtttat ctttgtaaca
    29581 catgatatgg atgaagcgat taaattagca gacaaaattt gtattatgtc agaaggtaag
    29641 gtggtgcaat ttgatacacc ggacaatatt ttaagacatc ccgcaaatga ttttgtacgt
    29701 gattttatag gacaaaatag actgattcaa gaccgtccca atgacaagac tgtagaaggt
    29761 gtaatgatta aaccaatcac gatacaagca gaagcaacac tgaatgacgc cgttcatatt
    29821 atgagacaaa aacgtgttga tactattttt gtagtagata gggataacca tttactagga
    29881 ttcttagata ttgaagatat aaatcagggt atacgtggac acaaaagttt acgagaaacc
    29941 atgcaacaac atatttatac cgttcaaatt gattctaagt tacaagattc tgtacgtacg
    30001 attttaaaaa gaaacgttag gaatgtacct gtcgtagatg atcaacagcg tttagtagga
    30061 ctgattacgc gtgccaatgt tgttgatatt gtatatgaca cgatttgggg cgatagtgag
    30121 gatacagtgc aagcagaata tgtgggggaa gacactgcgt cctcaaaagt gcatgagcaa
    30181 cacactacta atgtcaaagt acgtgacata ggagatgata aatcatgatc gagttcctac
    30241 atgaacatgg tggacagttg atgtcgaaaa cactggaaca tttctatatt tctatagtgg
    30301 cattattact tgccatcatt gttgcagtac ctataggcat tttattatca aaaacaaagc
    30361 gaactgccaa tattatatta actgtggcag gtgtcttaca aactattcca acactagctg
    30421 tacttgctat tatgataccg atttttggtg ttggtaaaac gcctgcaatt gtagcgctat
    30481 ttatttatgt attattacct attttaaata acacggtact cggcgttcaa aatattgata
    30541 gtaacattaa agaagctgga aaaagtatgg gaatgacaca atttcaattg atgaaggatg
    30601 ttgaattgcc gttagcctta ccgcttatac taggcggtat tcgcttgtcg tctgtgtatg
    30661 taattagttg ggctacactt gcaagttatg taggtgcggg tggattaggt gatttcattt
    30721 tcaatggttt aaatttatat gatccactga tgattgtaac tgcaacggta ctcgttactg
    30781 cactagcatt aggtgttgat gccttattag ctttagttga aaaatgggta gttcccaaag
    30841 gcttaaaagt atctggataa ttaggaggct aagataatga agaaaattaa atatatactt
    30901 gtcgtgcttg tcttatcgct taccgtatta tctggatgta gtttgcccgg actaggtagt
    30961 aagagcacga aaaatgatgt caaaattaca gcattatcaa caagcgaatc gcaaattatt
    31021 tcacatatgt tacggttgtt aatagagcat gatacacatg gcaagataaa gccaacatta
    31081 gtaaataatt tagggtcaag tacgattcaa cataatgcct taattaatgg ggatgctaat
    31141 atatcaggtg ttagatataa tggcacagat ttaacgggag ttttgaagga agcaccaatt
    31201 aaagatccta agaaagcaat gatagccaca caacaaggat ttaaaaagaa atttgatcaa
    31261 acatttttca attcgtatgg ttttgcgaat acgtatgcat tcatggtaac gaaggaaact
    31321 gctaaaaaat atcatttaga gacagtttca gatttagcaa agcatagtaa agatttacgt
    31381 ttaggtatgg atagttcatg gatgaatcgt aaaggcgatg gctatgaagg atttaaaaaa
    31441 gagtatggtt ttgactttgg tacagtgaga ccaatgcaaa taggtctagt ctacgacgca
    31501 ttaaactcag agaagttaga cgttgcatta ggttattcta cagatggtcg aattgccgcg
    31561 tatgatttga aagtacttaa agatgataaa caatttttcc caccttatgc tgcgagtgct
    31621 gttgcaacaa atgaattatt acgccaacac ccagaactta aaacgacgat taataagttg
    31681 acaggaaaga tttcaacttc agagatgcag cgcttgaatt atgaagcgga tggtaaaggt
    31741 aaagaacctg ctgtcgtcgc agaagaattt ttgaaaaaac accactattt tgataaacag
    31801 aaaggtggtc ataagtaatg gaaggtaatt tattacagca attattcaat tattatgtta
    31861 cgaactttgg ctatctatgg gacttatttt tcaaacactt attaatgtct gtctatggtg
    31921 tgctgtttgc agctttaatt ggtattccat tgggaatctt gcttgcaaga tacacaaaac
    31981 tttctggatt tgtaattaca attgcaaata taattcaaac agttccagtc attgcaatgt
    32041 tagctatttt aatgttagtc atgggcttag gctcagaaac agtagtttta acagtgtttt
    32101 tatatgcgtt acttccaatt ataaaaaaca cttatactgg tatagctagt gttgatgcga
    32161 atattaagga tgctggcaaa ggtatgggaa tgacacgcaa tcaagtgcta cgaatgattg
    32221 aattaccgtt atctgtttcg gttattatcg gtggcattcg tattgccttg gttgttgcga
    32281 taggtgttgt tgccgttgga tcatttatag gagcacctac gcttggtgac attgtgattc
    32341 gtggtacaaa tgcgacggat ggcacaacgt ttattttagc aggtgcgatt ccgattgcta
    32401 tcattgcaat cgtcattgat gtactattaa gatttttaga aaaacgatta gacccaacga
    32461 cacgacatcg taaaaatcaa tctaatcatc ggccgcaaag tattaatatg taatagtaga
    32521 agatgtttat aatttagcga ttccgtttca tgatttataa aaaatgaggc tactcaagga
    32581 gatcaaataa tctttgagta gcctttttat aggttgtgtt tgtatgcgtt tacactaaaa
    32641 tagaaattat tatcatgaaa gtttttggat aaaaagcgtt gattattgta aaaatactaa
    32701 aaaatgagat gttttattta taattttctg caaatttatg atattgtttc ttaatatatc
    32761 atattaaaaa tttgtttttc ttaaacatag gaggcttatc taattcatgg acacatcaaa
    32821 acaatttaga ggtgacaacc gattgctttt aggtatcgtt ttaggggtta ttaccttctg
    32881 gctattcgcg cagtcacttg ttaatcttgt tgtcccatta caatcaacat atagtagtga
    32941 cgttggaacg ataaatatcg ctgttagctt atctgcctta tttgctggtt tgtttatcgt
    33001 aggtgctggt gatgttgctg ataaatttgg tcgcgtcaaa attacttatg taggattgat
    33061 attaaatgtt gtaggttcat tactcatcat tattacacct ttgccagcat ttttaattat
    33121 aggtagaata attcaaggtt tgtctgcagc atgtattatg ccatccacac ttgctattat
    33181 taacgaatat tatattggta caagaagaca acgtgcctta agctattggt ctattggttc
    33241 ttggggtggt agtggtattt gtacgttgtt tggtggctta gtggctacat atataggttg
    33301 gcgttcaata tttgttgttt caattctatt aacattatta gcaatgtact taatcaaaca
    33361 tgcacctgag actaaagcag aaccaatcaa aggtatgaaa gcagaagcta aaaagtttga
    33421 cgttatcggg ttagtcattt tagtagtgac aatgttaagt ttaaatgtaa tcattacaca
    33481 gacgtcccat tttggtttag tttcaccgtt aattctaggt ttaattgtag tgtttatctg
    33541 ttcattagtt ggttttgtat attacgaaaa taaaataaag cacccactcg ttgatttttc
    33601 aatttttaaa aatagaggat acagtggtgc aactatttca aacttcttat taaatggtgt
    33661 agcaggtggt gcacttatcg tcattaacac gtattatcaa caacaattag gatttaattc
    33721 ttcgcaaacg ggttatattt cattaacgta tttaatagca gtgttgtcta tgattcgtgt
    33781 aggtgaaaag attttatctc aacatggtcc gaaacgccca ctattactag gaagtggctt
    33841 tacagtgatt gggttaatct tattgtcgtt aacattttta ccagaaatgt ggtatatcgt
    33901 atctagtata gttggatatt tattgtttgg tactggttta ggattatatg ctacaccatc
    33961 aactgataca gcagttgcta gtgcgccaga tgataagtcg ggtgttgctt caggcgtata
    34021 taaaatggcg tcatcattag gaaatgcatt tggagtagca gtatctggta cggtttatac
    34081 tgtgttagca gctaatttaa atttgaactt aggtggtttc acaggtatga tgtttaatgc
    34141 cttgctagca attgttgcat ttttagtcat tttactatta gttcctaaaa atcaaacgaa
    34201 tttgtaaaac tgaaatgaaa gcaagttatt atgtagggat tttaaaggaa attttgtgaa
    34261 agtaagttta tcatacacac ttaatgttgc gtattgacgt ttaatgttag gtgtgttctt
    34321 gtatagacga taaaagctgt gtgtatatta agcgaatgat tttcaaattg acgctaatat
    34381 gcgaaagtag tattttaaaa atgaacaaca acgatgaaga ggggtttata ggatgaaaat
    34441 tgcaattgct ggatcgggtg cattaggtag tggctttggt gccaaactat ttcaagcagg
    34501 atatgatgtc acacttattg acggatatac atctcatgtt gaagcggtta agcaacatgg
    34561 attaaatata acgattaatg gagaggcatt cgagttaaac attccaatgt atcattttaa
    34621 tgatcaaccg gacgaaagca tttacgatgt tgtctttcta tttccaaagt ctatgcaatt
    34681 aaaagaagtg atggaagcta tgaagccaca tattgataat gaaacgattg tcgtatgtac
    34741 gatgaatggt ttgaagcatg aagaaatcat tgcgcagtat gttgctcaat cacaaattgt
    34801 cagaggtgtt acgacttgga cggcaggtct tgaaagccct ggacacagtc atttacttgg
    34861 tagtggacca gttgaaatag gtgaactagt tgatgaaggt aaagaaaatg ttataaaagt
    34921 tgctgattta cttaacgaag cggaattgaa tggtgtcatt agtaaagatt tataccaatc
    34981 gatttggaaa aagatttgtg ttaatggtac ggcaaatgca ttaagcacag tgttggagtg
    35041 taatatggca tcactgaatg aaagtagtta tgcgaagtgt ttgatttata aattaacgca
    35101 agaaatagtg catgtagcga cgattgataa tgttcattta aatgttgatg aagtatttga
    35161 atatttagtt gatttaaatg aaaaagttgg tgcgcattat ccatccatgt atcaagattt
    35221 aattgttaat aatagaaaga ctgaaattga ttatattaat ggcgcagttg caacattagg
    35281 taaacaacgt catattgaag caccagtcaa tcgctttatt actgatttaa ttcatactaa
    35341 agaaagtcag cgacatgcac aggattaatt agtgttttta ggtgtgattg caaggaaatg
    35401 acaatcacgt gatattacgg tcattattaa aattgaaatg taataaataa agaacagcag
    35461 taaagtactt tcaaattgaa atgatcttgg tgctgttttt cttgattggt cttcgtcata
    35521 attcagattt gtcataggtc tacgacatac tactagcatt tgctagacag tttttacgac
    35581 gacactttga aaaaaattga ggcaaatcat ttggaagtct tacgtgaatt ttgtacactc
    35641 atcaagcaag taattatatt aaaagacaaa tagagaaaag gtgtttataa tgagtaaaat
    35701 ttttgtaact ggtgcaacgg gccttattgg cattaaatta gttcaaagac taaaagaaga
    35761 ggggcatgag gttgctggtt ttactacatc tgagaatggt caacaaaagc tagctgctgt
    35821 taatgtaaaa gcatatattg gtgatatatt aaaagctgat accattgatc aagcgttagc
    35881 agattttaaa ccagaaatca ttatcaacca aattacggat ttaaaaaatg ttgatatggc
    35941 agcaaatacg aaagtacgta ttgaaggttc taaaaactta attgatgcgg cgaaaaagca
    36001 tgatgttaag aaagtaattg cccaaagtat tgcctttatg tatgaaccgg gcgaaggatt
    36061 agcaaatgag gaaacttcac ttgattttaa ctcaactgga gatagaaaag taacggttga
    36121 tggtgtggtt ggtctagaag aagaaacggc ccgtatggat gaatacgttg ttttacgttt
    36181 tggctggtta tatggtccag gtacttggta cggaaaagat ggcatgattt ataatcaatt
    36241 tatggatggt caagtgacac tttcagatgg cgtaacatca tttgtgcatc ttgatgatgc
    36301 agttgaaaca tctattcaag ctattcattt tgaaaatggt atctataatg tagcagatga
    36361 tgcacctgtt aaaggttctg aatttgcaga atggtataaa gaacaacttg gtgttgaacc
    36421 aaatattgat attcaacctg cgcaaccatt tgaacgtggc gtaagcaatg agaagtttaa
    36481 agcgcaaggt ggtactctga tttataaaac ttggaaagat ggcatgaatc caattaaata
    36541 atcggtttct gcatatagca tgttaataat gaagacttgt tcaaatcgct aatcctcata
    36601 cataaatatg tatgtttcgg attgatgtgc gattgaacaa gtctttttta tggtttctta
    36661 attgtcgttg tatgtaatat taacagaagt gtaattaaca tactgaataa tgcaataaat
    36721 gctgtagtta acgttaattt aatttctatt ggcagccaag tcagtacaaa agaccagtta
    36781 ttgctaccga gaatgagata tggtaatgca tataatatga gtgctatagc gatacataaa
    36841 cataaagtta gccaacttaa aaatgtggta agttttgatt tgcgcaggat gattttcccg
    36901 tgacgcattt gccaagcacg ataagctgag aatataaatg ctaataaaat aagtgttgtc
    36961 atcaaaacgg taacaatatt aaattgatct ttatattgat tgagcataga cgcaactgtc
    37021 gaatatcgct tgtgatttac aatttgtgtg ttaagatgct caactaattt gggtacatat
    37081 tccgaattta gatttgcaag cacaacaatt ccataatttt gttttggatt tagtaaaata
    37141 aatgatgaaa agttatcgag cgttcctgaa tgaaacacta aatgttcatc attattagta
    37201 aaccagccgg aagcatatgc attggcatta ggttcaccaa ttgttgatga taaattttta
    37261 tgtgattgtt gaactaatga tttgtattta tcaggtggat taagttggaa ttttatccaa
    37321 tgttccaaat cttcagttga tgtcatcata tatgctgatg gtgtatccca aaggttaaat
    37381 tcaggtttag agacgacagg tgtcgaacct tgtaattcgt agccaatagc atcatgtttt
    37441 gatttgttat tgttttgttt gaatgatgta tgtgtcatat gcaaaggctt gagccatgaa
    37501 tttgtaatat attttgtata ggattgcttc gtaacgtttt ggataattaa acctaataaa
    37561 tcatagttca tatttgagta ttcaaattct tctccgggct tatgatgtaa ttcatcaccc
    37621 ataattgcac gggttacatc atttaaacga ttatttttat ttgttacgga gtcttcgctt
    37681 gtaatatcac taggtatacc acttgtttga gccaaaagtt gcttaatcgt aatagtttca
    37741 ttttgaccat tatagttcat tttaaaatga ggcacatgtt tggatacgtc atcatttaag
    37801 tttaatcgac cttcttgagc taattttaaa attgcaagac ctgtgaaagc tttcgtatta
    37861 gaagcaattt catactttgt tgtgggagaa gctttgactt ttttatcaac atcttgataa
    37921 ccataacctt tatttaaaaa aactttgcca ttttttacta ttaaaattga tgctccagga
    37981 atatgtccct tttgtaaatc atgttcgata attgtatcta tttgttgttg cgaatcattg
    38041 gttaaccgtg tcttcgtatt gctatttaat gttatatata tagcaattga aatggcgact
    38101 aaaatgataa tagaaatgga tagaaaatac agttttttag tagtcataaa cattcctttt
    38161 tatatgttaa atttacttaa gtgcctaatg agatttattt tattatacca gttctattga
    38221 ataaattaat taaagaggta tatttaccat tatttttgac accttatatg ttaataggtg
    38281 tataattaca taaaaaatat taattaactc tctatacttt ttggaaggat aggcgtatag
    38341 tgatatccat caaaaagtat aaaaaggaat aggagattat gttcaatcaa tttaaaagac
    38401 ttattatagg gcaacctaaa aaaaacagag aactaaaaga cgaaaagata tcgaagttta
    38461 aagggttagc gattctttcg tcggatgcat tatcttcagt ggcttacggt cccgaacaaa
    38521 tactgattac attatcagta gtgggtgcag ttgcaacttg gtatacttta ccgattgcag
    38581 gtgctgttct gatcttatta gctgctttaa ttatgtctta taggcaaatt atttatgcat
    38641 atcctaaagg tggcggcgcg tatatggtgt ccaagaccaa tttaggagaa aaatggggat
    38701 tgcttgcagg aggctcatta ttagtcgatt acatactgac tgtcgctgtt agtatatcat
    38761 ctggtgctga tgcatttgtt gctgcattcc caagtttata tgggcataaa gtactgattg
    38821 catgtttact tgtacttttt attttaatat taaatttacg tggtttaact gaatctgcca
    38881 ccgtattatc ctatccagta tatcttttca ttatagggtt agtgatatta atatttatag
    38941 gtactttccg tgtggcgaca ggtgatattc aaccacatat gcatgcatca gtaggaactg
    39001 cggttcctgg agtaacatta ttcttgctat taaaagcatt ttcatcaggg gcttcatcat
    39061 taacaggtgt tgaagctatt tcaaatgcgg taaccaattt tagagaacca agtgctaata
    39121 atgctgtaaa aacgttaatt gctatgggtt caattttagc gtttttatta gttggtatcg
    39181 ttggtttagc atacgtttat ggaattatgc cacaaacgga aacgacagtt ttatcacaat
    39241 tagctatgca aatatttgat gataatgctg ccttctattt tgtacaggca acgaccgtga
    39301 tgattttggt attagcagcc aacactggat ttacagcttt tccgatgtta gcggcttcaa
    39361 tgtctaaaga taaatatatg cctagaatgt ttactgttag aggtgatcgc ttaggctact
    39421 ctaactcgat tattatttta ggtgtattag ccattatttt aattattgta tttgatggta
    39481 tgactgaaga tttaattcca ttgtatgcgg ttggtgtatt cattccattt acactagcac
    39541 aattcggtat ggttattaaa tggattcatg aacgtcctaa gaattggtta agtaagttgt
    39601 ctgtgaactt acttggtggt atcgtgacat ttattgtttt tatgatttta ctcataacta
    39661 aatttagcca agtttggccg atacttattt tcttgccgtt cgtggtgatt ttcttcttga
    39721 aaattaacaa acattatcga gatatcgcag aacaattacg ttctgatatt gatgttcata
    39781 atgtagaagt agtagatcgt aatttagcaa ttgttccgat aacaagcatt acaactgcag
    39841 ttgataagtc tatttattac gcacaaatgc ttgcgaataa tgatgttatt gcagtacatg
    39901 tatcatttgg agatgaagat gaaaaagcat tccaagagaa atggaaacgt catttcccag
    39961 atgtaagatt agttatttta cactcggaat atcgcagtat tatacgacca atttcacgtt
    40021 ttatcgataa gattaatcgt aaagccaacg atcaaaacta tatgattaca gtagtgatac
    40081 cagaatttat tactaagaaa cgttggcata atttattaca caatcaaact agtttgcgta
    40141 tgaaattgta tttaatttat caaaagaatg ttaacgtatg tacaattcca tttaaactta
    40201 aaaaatagat tctataatca aatcggattg atgaaaggtt atttactttt catcaatccg
    40261 attttttgat gttgactata aaaaccccgt acatatgaga tgataatgac tttgtctcat
    40321 atgtacgggg gtgttgttat gtcttgctta atctttggac tgtaaagaca agacaatttc
    40381 gttgtaattg atattttctc gaatttgagc tgcaacgata ttgttgattt tatttttgcg
    40441 aattaattca tctaaaagat gacgttgtaa gtacaatgct tccaatttgt ggttatggaa
    40501 catagcttgc ggtatgattt gattgctgtt ttcttgacgt tcttttcgac gttgtgcacg
    40561 ccactttaat gagcgactca tatcatagta atgattaatg actaagctaa cctcaagtac
    40621 atttgtacta ttttgttctt cttttaagcg taaaataata ttgtgattca caatacgcat
    40681 aattttttga acatctttga atgaattttc aaattcttgc ttcttagatt ctttgtttaa
    40741 ggctttgtag tgcttcatcg ctttaagtct aagccataac atcttgaaga agtttgcaaa
    40801 tttttcaaca aatgatgcag tacggtgtgt ctctgttaat tcgacaatat tgcggtaatt
    40861 attaatatcc tggtatgttg ccttaccttt atcgattaaa cgctcaagtg tagatgtttc
    40921 gattactttt gcaatatctt ctaaacgttt gagttctttt gtattttgat tatcaggttc
    40981 tgaatttaat aaaaataaca attctccata gtattggttt aatactgggc gataatttgt
    41041 atcgtttttg tcttttttac tttcgttttt aaaatgctgg atcacttttt gaacaatgaa
    41101 aattttggca gattgataag tcataccttt aaaagtagta tcttcggcag atggtgtaat
    41161 taaaggtaaa acaatttgcg ctaaaattaa actaattaat accatgaaag atgcaataaa
    41221 caatagatca ttacggtatt caaaagcttg cccttgtgta ataataaacg gcaatgtaag
    41281 tgccattgaa agtgaaatgg taccgtgaat accacacatc gtcataataa atgcgtaacg
    41341 cacacgagaa ggtggtgttt catgtgaatc atgttcctcg tctagataag attgtatatt
    41401 tttcgggaaa taaaaatctt tgtaccagaa ataaacccaa acaaatcgac atgcataaat
    41461 tgcaatagcg attaatatag tgatcactat taaaaatgaa aagttgtcag gctctgtttg
    41521 gaaaatatct atgactacag taggaatcat aaaacctaat acaacaaata caaaaccatt
    41581 taaggcataa ctaaatgtgt tccaaatttg atggtaattc atttgtagtt ccgtttgtgc
    41641 tctaattaaa cggtcgcgtt ctaaaccatg gattagacct gcgattacaa ctgcaatgat
    41701 acctgaagca tgaacttctt ctgctaaaaa gtatacgaca aaaggagtta ataattgaat
    41761 aaaagttaag gtattgttat cttttaaacc tttattagcg gttaagtcta tacgtattct
    41821 aacgacaacg aatccaataa ttgcaccaat aagtacacct agtattgttg aaatgataaa
    41881 ttgttcaata gcttggaata atgaaaaggt accagttact aatgctgtaa cagcgatttt
    41941 gaaagaaatg atacctgcag catcattgag taaagattca ccttctaaaa tcgtcataga
    42001 acctttaggt aataattttc cgcgtgtaat agcagatact gctactgcat cagtaggaca
    42061 taaaattgct gctattgcaa aagcggctgg cattggtaag gcaggccaaa tccaatgtat
    42121 aaaatagcca acaccgacta cagttgcaaa cactagtgcc attgacatta atagtatagg
    42181 tttgcgatat tctaataatt ttgttcgaga gacgtgggta ccttccacaa aaagtagtgg
    42241 cgcgataacg gcaaacataa atacttcaga attgaattgg aaatcaactt gtattggaat
    42301 aatgaaaata acgacaccta atgcaatttg aataaaggca gtaggaattt gtggaaatcg
    42361 attattgata accgaactaa taatcacagc aaaaataaaa attaaaaatg cttctaatag
    42421 tgccatacaa tactcctcaa aattttaata gttaatattt tatcactttt aaggcataat
    42481 gacatagata tattgataaa atgaagttat tttcaaaaaa actctagtat cggttgaact
    42541 gatactagag cgagatgttt aaattattga ttgtcatatc tgaaatgacc gctgtcattt
    42601 tgtcgttgtt catatgcgag cttttcagca ttcgttttgt attttttata aaagaaaaat
    42661 aaaaatataa accagaatgg cgaaatataa atagctgctc ttgtttcgtc actaaagaat
    42721 aataaaatga atacaaagaa gaagaaagct aaaataatgt aagcaatagg cttaccacca
    42781 atcaacttaa atttactgtt tttatgtgcc tcaggatgct ttttcaaata cattagataa
    42841 gcgacaataa tcattgccca aacaactaaa aacaacacag ttgataacgt tgtaacgtat
    42901 atgaatagtt gaattgcatt aggaaaaatg tagtttaaca aggctgctat aagtaatgca
    42961 attgatgaaa ctaacattga taaataaggc acgccatgac tattcgtttt atttaaaact
    43021 ttaggaccta acccttgttg tgacagtccg aataagatac gactattcga atagattcca
    43081 ctatttgtag cagaggccgc ggcagttagc acgacaaagt taacgacacc tgctgcaaat
    43141 ggcacgccga ttaatgtaaa gagtttaacg aaagggctac tatttggatc gatatcattc
    43201 caaggtatga ctgacataat tactaataga ccaccgatat agaataataa aatacggata
    43261 ggtacattat taattgcttt cggtaaagtc ttttcaggat ctttcgtttc gccagcagtt
    43321 acacctataa gttcaatacc aatgaatgaa tatacagcaa tttggaatga cattaagaaa
    43381 ccaaatgtcc ctccagggaa catgccgccg tgactgatta agtttgtgaa tgatgcatgt
    43441 ccataatgtg ttttaaatga gaaaaagatt aatacaagac caacgataac catcgcaata
    43501 attgtaacaa ctttaataat agagaaccaa aactccagtt caccaaataa tctggcacct
    43561 aataagttga agctaatcaa gattaaaaca ataaatagca cggtaatcca atttgggact
    43621 tgtggatacc aaaatgcaaa gtattgtccc atcgcagtta ggtctgacat acttgataca
    43681 atccaacata gccagtaagt ccagccaatg acaaagccac caaaagggcc taaatattca
    43741 tttgcaatat caacaaatga attaaatctt gtattgctca acaacaattc gcctaatgcg
    43801 cgcataaaag cgaatagtac aaccccaata atcatgtatg tgaataacag tgatggacca
    43861 gttaaggata ttgtttgacc tgatcctagg aataacccag ttccaattgc gcccccaatt
    43921 gctatcaatt ggatgtgtct gttattcaac tctctatgca attttcttgc cataattttc
    43981 ctcctcagga taaagcaaat aaaaaagttg tgtgtaagcg ttttcttaat tataaatact
    44041 aaagaaatcc atttaataaa gcaattattt ttttaaatat aagaaaaaac atttgatagt
    44101 taagggtatg ataactaaaa cttatcgatt ttttgaaaag atataagttt ttatgcaaaa
    44161 atataaaata atcatctaaa tgaagcagtt aactatatat attagataag ctatggcata
    44221 ataaataaca ttttaatttg agtaaagaag gtatgggtat gaagataggg acaatcgccg
    44281 atttacatat tgatcgtcat aataaaaaaa catccgaaga ttacttagag gcattggttg
    44341 aaatagttaa gtataaaaat ttggatatat tgttgattgc gggggatatc tcaaatcatt
    44401 atcaattgac gcaccaattt ataacgcaat tgacaaaaca attagatata cctgtgaaat
    44461 ttgttcctgg aaaccatgat ttatgggaag ttgaaagtat gactacgcaa gacatttgga
    44521 ataattataa gagtatgtca cagtgcttgg taggaaaacc atttatagta aatgaagaat
    44581 gggcaatcat aggacataca ggttggtatg attatagctt tgcagcacaa cgattttcat
    44641 tagatgagtt acaaagagga aaacattatg gtgcgacttg gcaagataaa gaacgaatat
    44701 cttggggcac atcagatcaa aatttatcta aaatagcggc tgaacaagtg aagaaagatc
    44761 tattagaagt aggaaataga agagtgattt tagtcacaca tgttgtgacg caccctgatt
    44821 tcattgttcc tatgccgcat cgtatattcg atttttataa tgcatttatt gggacaagtg
    44881 atttcaatcc tttgtatgcg atgttcgata taccatatag tattatgggc catgttcatt
    44941 ttcggaaaag tgtgatggat gatggcagat gttatctctg tccgtgtcta ggctatccaa
    45001 gacaatggcg ttcagaagat atttaccagg aaataaatga gacgatacaa ataatagaaa
    45061 tttaaaatgc gcaaacctga cccagtttgc gcattttatg ttttacacac gcgagtaatg
    45121 tgtttactta cgtgtgttta ttttgtttct gattttcaat tgtatatgaa tgtggttgca
    45181 cataaatgca ctttcttcct ggtgaattaa agctgtattc cattttctct ttacgaattt
    45241 taataatttg tttgcgattt ggaatgatgg caggtaaaac taggccacga cgaatatgac
    45301 tccaaagtgg atcgatataa tagtaatagt aatcatcgta cccaatgagt aacgttacgt
    45361 gaatatttga aacttgtttc gctggcttgt tatcaaagcg gaaaacacgt agtaatggtt
    45421 tagaaccaag attagtatgg tatattaaca caggttgacc ttgatcgata atacctttaa
    45481 gatcttctaa ggatttacca gtgccgtcta cgatattagg attgtatttt tgtaaaaatg
    45541 gtacatatgc atctggaaat attgtttgat gataattgcc aagcttaatg aataagtgat
    45601 gtccaacata acctttatgt ggattgttcg gatgtgtcgg ccaatgtctc ataatttctg
    45661 tagcagggat atgttggtta ttgtattgca acatcatggc tgcggaaaca ccttcacacc
    45721 ccatgaccat aggaatagga aatagctgac tgataggttt aactggtaat atttttcggt
    45781 tcataatata gtcctcgcat tgattcaata aatatttaat ataattatat agcgtcaatg
    45841 caaaatgtcc taaacatatg ttttacatga gtgaataaaa ttaatggagt gataaaatgg
    45901 aatatcaatt acaacaactt gcgtcgttaa cgttagtagg tattaaagaa acgtatgaaa
    45961 atggacgaca ggctcagcaa catatagcag ggttttggca aagatgttat caagagggag
    46021 taattgcaga tttacagtta aaaaataatg gtgatttagc cgggatactt ggcttatgta
    46081 tacctgaatt agatggtaag atgtcatata cgattgcagt taccggagat aatagtgctg
    46141 atattgaaaa atatgatgtc ataacattag caagttcaaa gtatatggta tttgaagcac
    46201 agggagcagt acctaaagca gttcaacaaa aaatggaaga ggttcatcac tacatacatc
    46261 aatatcaagc agatacggta aaatcagcac cattttttga gttgtatcag gatggtgata
    46321 ctacaagtga aaaatatatt acagaaattt ggatgcctgt taaagggtga ttgaatagaa
    46381 atgtacttga tatgaaggcg taattttagt tgaagattca atctcataat tcataaagaa
    46441 gaaccctgac aaagatagat atctttgcta gggttctaat tatgttgaag tgttatgcga
    46501 catgttgctt tttaatcatt ttaaagtaaa tgatttgtag actgactaat agaatggctg
    46561 taatgaagat aataatcata tatggtgatg tattaaattc gccttttaag ccaactaatg
    46621 gtgcaacagc gccacctaag ataaattgga ataagccaag taaacttgat gcgttaccac
    46681 tgccacctgt tcgttcttcc atagccattg tgaaaccaag cggtccaatt gacgtcacag
    46741 gacacacatt taagaagaat gcgatgagta agacccaaag tggtaaatgg aatgtaagtg
    46801 tcaggataat taaagcaaca cctaccactt gtataatagt taaaatgatt aataatatgt
    46861 gacgatgtaa tttttctact aataaagcaa cgacttgact gacaatgatt aaacctacac
    46921 cgttaacagc aaacatgata ctaaattgtt ggggtgtcat attatacaat ttttgagtaa
    46981 taaatggcga tgcagatgaa taactaaata gcattacata agttaaacct tgtaatagca
    47041 ttggaataat aaatgctggt tttttcaaaa gacttccaaa gtctttaatg acgctactaa
    47101 aattcacctg ctttaattct tctttaggtg ttttaggtaa ttgagaagcg acgccaatta
    47161 aaatgatgag tgccacaatt gttaaaattg taaaaataga acgccatgtt gctacggata
    47221 aagctaatcc accggctaat ggtgcaagaa tagtgatgat gccattaact accattaaag
    47281 atgctaaaaa tttagcgagt gcgttgccac taaatttatc accagcagag gctttggcaa
    47341 taacgatgac gccaccacct gtcaaacctt gaataaatcg taaagttaag aataatggca
    47401 attgatcaac aaacatagac aaacctgatg ccaaaatgaa aatgattaaa atggatacag
    47461 caatacgttt gcgaccaaaa gcatcggata aaggtccaaa gataaattgg cctagtgcaa
    47521 gaccaatcat tgtgaatgat aatgttaatt gaatttctga tgtagtagaa ccaaaatcac
    47581 cttgcacttt aggtaatgat ggtacgtaca tatctatagt tagagggcca aaagcggtca
    47641 tgacacctaa cgtgattagc aataaaaatg aaaaatgctt cgttttggtt ttcgaagatt
    47701 gcataaacag actccttaat ttatataatt atataaatta tatacgtttt gtgtgtattt
    47761 gtatgtagtt agaatacaag tttatataaa aaatccctat actcttaatg catcatcacg
    47821 aataacgtag tgatacagat aaagtatagg gaattaatgt ttatcatcaa aagcaggttt
    47881 aataattaat tagcgccacc attgtcatct ttaaataagc tttctaattt agatttaata
    47941 ctgtcgaatt ttgtattaat atcatttaac atgctttcaa ttttaccttg gcttgcattg
    48001 tttttaagtg cattatttgc ctctgtcaat ttgttgattt ggtcttgata aaattgagtt
    48061 agcttagaat cagctttgtt ttgttcttgc tgcttcaagt tatcgatttg attttgtaaa
    48121 tttttaataa tgtcatcagt tttttggttg tttttaatat tttctttagc attatcgact
    48181 tgctggttaa tttggccgtc ttgattttca acttgttttt tataattttg agagacttta
    48241 gggtcattac taactttatc gctatcattg ttaagtaagg cgcgtgtgac aaaaatagcg
    48301 acaataatta agagaatggc aataatgata tataaccatt tttttgaacg gcgggatttc
    48361 tcgttttctt cttcaagaat ttgttctctt cgataatctc taccgttata gtatcgttct
    48421 gggtgttctt caggatcacc tttagcatat tgttgtctat acgatgcgtc ttcagattga
    48481 cgacgatgat tttgattgtc gtattgatat gaatcacggt atttatcagt tcttttcatg
    48541 ggtttcacct cattcaagtt ttaactgcaa ataataaagc tgatgattta ctttgacatt
    48601 attgtaattt cagaatacga gcaatgtcag ttgcagtgtt taataaattt tgttcgctat
    48661 tttgtaggac atcttctaaa gtgctaggtg aagagattat agaataggca ctatcaatac
    48721 cgaaatcgta aacatgttga taattttcgc ctagactgcc acaaatcgcg atgacaggaa
    48781 tatgatattg ttttgcagct aacgcaacgc ctacgggtgt tttaccaaag atggtctgat
    48841 aatccatgcg tccttctcca gtaataacga ggtctgcatc tttaattctt tgatgaaaat
    48901 ctgtaatgtc aaagacgaca tcaatacctt ttgttaaagt tgtctcacaa aacgctaata
    48961 atgctgcgcc cataccgcca gctgcaccag aacctggtat ttgattaact gactttcctg
    49021 tgcacatttt tatcttatca tgataatgcg acattgcgaa atccaacttt ggtatcatct
    49081 ttgcatcagc gcctttttga ggaccataaa tataggtagc accattttca cccaataaag
    49141 gatttgaaac atcgcaggcc actttaaagg tcacttcttt taatcgcgaa tctagattgg
    49201 ttatatcgat ttgtgcaatg tgagcaagat tatcaccatt catttgtaat aagtccccgt
    49261 ttacatcagt aaactttacg cctagtgcac ttagcatacc tgtaccacca tcatttgttg
    49321 cactgccacc aatccctaaa ataatggtct tagcaccatg atttaatgca tctttaatta
    49381 gttcaccggt accatatgat gatgtgtata aaggattacg ttcctctttt tctaataaat
    49441 ccaaacctga cgctgctgcc atttcaataa ttgcaatttg ttgttcgtct gcgcgtgcat
    49501 aacatgcttc aataggtcgc attaaagggt cattaacgat gactgtatac ttagtagcac
    49561 ctgttgcatg cattaaagca tctgtggtgc cttcaccacc atcagccatc ggaatgatat
    49621 cataatgaag ggttttccca taaacattag taaatgcctg ttttataata ttgccaactt
    49681 gctgtgcggt catactttcc ttaaatgagt caggagcgat gacaattttt tgtaattgca
    49741 tagtagacat cctctcatta ctgttgtcgc tcttattgta tataaaatta ctagtgaact
    49801 aaagtaatct ctatagtgct aatgagtcgc gcgataaatc agttacatta taatggcgta
    49861 taaggatgag gggaaagaga ctaaatttgc tgtgaaaggg tataaaaact gaaataagta
    49921 tatttgaaga gaagcggatt ggctcgcata ttcgagtatg ttggagaacg cagttataca
    49981 tatattttga tgttgaggag ttacacatat gaaactaaca caaacgcatg ctgaaatttt
    50041 aaaatttatc attgttggcg gcattaatac gttaaactat tatgttgtct atttattgct
    50101 gttaaaatta ttacacattg aatatatgat tagtcatatt accggattta tagtcgcttt
    50161 tgtgatttcg tattatttga attgttattt tgtttacagg gtaaaaccta cttggagaaa
    50221 attcattagt tttccaatta cgcagattgt caacataagc ttacaaacag ttttattata
    50281 tgtatttgta tcctggctaa atttgccagc agaaattgca ccatttgctg gtttaattat
    50341 tacgataccc atcacattta tattatctaa atggatttta aaagatagta attaaagtag
    50401 cgtaaaccac taaaaaatac tttggaaggg tcttaatatt gcgtgaaata taataaataa
    50461 atgatataat ataatggtcg cctaacatct cgggcgttac tttaacgcag agaggaggtg
    50521 tatgacggat gttcaattta ttaattgaca tcatgacttc agctttaagc ggctgtcttg
    50581 ttgcgttttt tgcacattgg ttacgaacgc gcaacaataa aaaaggtgac aaataaccaa
    50641 cacctaaata aatcccctca ctatggcagt agtgagggga tttggtgtat ttagatgttc
    50701 aatttattat gtaactaatt atagcattga tgaacaaggt gtgcaatgta tttttgcaat
    50761 attacaataa atggatttaa ttacattaga attaagtatt tcgaaaaatg atttaatagt
    50821 tgagtcatag gatcattaga taaagcgaat aatcaattat agggacattg ggatacaaat
    50881 caatgtacta gactctacaa ggctatactc agggacccaa catagagaaa ttggatccac
    50941 agtttctaca gacaatgcaa gttggcgggg ccccaacaca gagttttact catgtattcc
    51001 tatttttaag tacatattag ctgtggctaa ggataaagaa tcattacata ataaatcatt
    51061 agtggctctt tatcatttct gccccataac cttgtgaatt tttaaaagaa atgtgtcaca
    51121 aatccacgcg acattgataa tcgttatcaa ctaatatatg atgtacttgt aataagtatt
    51181 aatttttaac aaataacaaa tttatattat gtatatcatc cagtgaaata aataatataa
    51241 gcggcttaat ttagattatt aattgaaagg agttacatat acgatgaaca atcagacaaa
    51301 ttggataaag atactcagcg gatttgcttc tgatagtaaa tggaaaatta tgttatcaat
    51361 tttgttgtct atcatcagtg tcttttctgg attagttcca tattgggcag tctttaaaat
    51421 tattttaatg atgattaaca atgcatatac gattaattcg attatggttt atatctttat
    51481 tgttttaatc gcttatattt cacaagtgtg ttgttttgga gcgtcaacga tgctatcaca
    51541 tattacggca tatgaaattt tatctgaaat tcgtaaaaaa ttagctcaaa aattaatgcg
    51601 tctcccttta ggcgtagtgg aatctaagaa aataggtgaa ttaaaaaata tatttgtcga
    51661 taaggttgaa acaatagaat tacctttagc acatatgatt cctgaagtta taggaaactt
    51721 acttttgtca gctgctatct ttttatacat aatgctcatt gattggcgta tggctagtgc
    51781 cttattagta acgataccaa tttcaatttt cgcttttaaa aaagttatgt ctggatttaa
    51841 tgagacatat gctgaacaaa tgaaatcgaa taattatatg aatagcgcga ttgtagagtt
    51901 tatcgaaggc attgaagtga ttaaaacatt taatcaatct caaagttcat ataaaaaata
    51961 taaagatgca gtagataatt ataagattca cactttgaat tggtttaaaa atacatgggg
    52021 gtatatgaat ttaggagcta gtgtattacc ttcaacattt ttagggattt taccggtcgg
    52081 catgtatttg atatctatta accaattaaa ctatgcggaa tttttcctat gcatcgtatt
    52141 atctttaggt gtagtagcac cgattaagaa ttttactaat tatgtaaatc atttaaagtc
    52201 tatacaatac gcattaactg aagtgaatca aatattaagt ctagaagagt tagtattgtc
    52261 aacaaaattt aagaagcctc aacattatga aattgctttt aataatgttg gattttcata
    52321 taacaaagat aaggacgatc tggtctttaa gcatttatca tttacagtac cagaaaataa
    52381 tttcatagct atcgtcgggg catctggtag cggtaagtca accattgcca agcttatatc
    52441 acgatactgg gatgtgactt caggtgaaat tacgattggc ggtatagata taaaagatat
    52501 tgaatcgaaa caacttaacg atttagttgg ttttgttgga caagataact ttttattaaa
    52561 tcttactttt aaagaaaata ttaaacttgg caatccagaa gctacggatg aggcagttga
    52621 aaaagctgcc aagttagcac aatgtcatga atttattgaa aagttgccag atggatatga
    52681 tacgaatgtt ggtacagtgg gagataaatt gtctggtggt gaaaaacaac gagtcactat
    52741 tgcaagaatg atattaaaag atgcgcctat cattgtatta gatgaagcga ctgcttacgt
    52801 tgatccagac aacgaacaaa aaattcaaga agcattaaat gtgttgacgc aggataaaac
    52861 attgattgtt attgcacatc ggttatctac gattcaacat gcagatcaaa ttattgtttt
    52921 aggtaaacaa caaattttag aaaaagggtc acatcactta ttgctaaaat taaacggtaa
    52981 ttataagaaa atgtgggata cgcacatgca tacaaaagat tggggaatca atactggaca
    53041 taattaaact aatgagaaaa ggaggtcaaa tatgtttcaa attacattta aaattttaaa
    53101 ctggataaga ccatataaag caagaatgat tttaggattt agcatgtcat ttttaaatgc
    53161 tatttttatt gcgttaccta tctttttagt tgcaaaaata tttaataatg tgctatctca
    53221 taaacctatt tatatgaaag atatattgaa tgttgtaatt atcatggttt tattagtgat
    53281 tggacgattt attacagcat atttcaaaag caaaagccat gagagcatcg cttatgaaat
    53341 gagtgcgaaa gaacgtttag atatagggga taaattgaaa aatgtaaggt taggttattt
    53401 taattcgcat cattcaaatg agttaacaac aatagtaaca acagatttaa cctttttaga
    53461 aaactttgct atgaaaatgg tggacgttgt tgttaatgga tacatattaa ttacagtact
    53521 catattgtct ctacttgtag tttcgtggca agtatcatta ttagcatgca ttggcgtatt
    53581 actatcattt tttgcgattc aattattaga aagaaagagt cgacaaaatg cgccagcgta
    53641 tcataatgta caaaaccaat tagtggaaaa ggtattggag gttattcgtg gtattcaagt
    53701 aataaaatca ttcgcgaaag aaaatacgag tcttaaaagt tttaaccaag cagtcaatgg
    53761 aagtaaacgt ataaatacaa aaatagaaat gcaatttatc ccattcaatt tattgcattt
    53821 acttagttta aaagttgttt caataatgat tgtattagtt gcatgcttat tatatatgaa
    53881 tcatagtatt gatttaccta cccttattat gatttcaatt ttttcatttg tgatatttga
    53941 tagtgttgaa aatattaata gtgctgcaca cgtacttgaa atgatagata tgacgataga
    54001 tgatattgaa aagataaaaa atgctccaga actggatgag aatggaaaaa atttgacgat
    54061 taaaaatgaa aatatcgctt ttcaaaacgt gaatttttca tatgatgata aacaagtgat
    54121 aaagaatgtg aattttgaga tacctacaca aacatcaaca gcaataattg gaccttcagg
    54181 aagtgggaaa tctacattat gtcacttact cttgcgcttt tatgatatcg atgatggaaa
    54241 tattcgcatc gatggtgttg atattaaaga tatgacatta agtacgttaa tgtcgaaaat
    54301 tagtgcagta tttcaaaagg tgtatttatt taatgatacg attgaaaata acatattgtt
    54361 tggcgatcca ggtgcaacga aagaagaaat tattcgtgca gcgaagcaag catgttgcca
    54421 cgacttcatc atgtcattac ctgagggata tcaaacaatg ctaaatgaaa aaggtagtaa
    54481 tttatctggc ggagaaaagc aaaggatttc tattgctagg gcgatattaa aagatgcacc
    54541 aataattatt ttagatgaag caactgcaag tattgaccct gaaaatgaac agctgattca
    54601 aacggcaatt aatgaattaa gtaaaggcaa aacagtaatt acaattgcac ataaacttga
    54661 aactattaaa aatgcagatc agattatagt gctcaatgaa ggtgaaataa ttcaaaaagg
    54721 tagtcatgat gaattaattc gaaaaccagg aatgtatcaa gactttataa gaataaaaag
    54781 taaatcagca ggctggaaat tataaataat agtttaatat ggaatcagaa tttggaaata
    54841 aaaagggtga tagataatca acagcaaaat aaatcccctc actagggcaa tagtgagggg
    54901 atttggtgta tttagatgtt caatttatta tgtaactaat tatagcattg atgaacaagg
    54961 cgctcaacgt aatattacaa ttaatggatt gtttcacaac atggtaaagc taggatgtct
    55021 ttttattaag aaataatgca attattaatt ttaactgaca tattttatcg aatcgctgtt
    55081 tatatctgcc cgaattctta gtcatttcaa tagcaaggtc aaaattttgc taaaatttat
    55141 agttatactg agaagtttaa attcatgttt tcttgaaaaa cacgcgcctt aaatgtaaac
    55201 ttattaatta taaaagttta cattcggatt gaggtgctta ttttttatga ggatttttat
    55261 tacaagtacg aatactgatg taggcaaaac ctatgttaca aagcatttat accatgcttt
    55321 gaaaacacgt ggtcatcgag tttgtatttt taaaccattt caaactgagg aacgccaaga
    55381 cgggacattt ccagatttag aagtatttaa aaatgaatgt gatttaagct atgacataac
    55441 gtcactttat acttttaagc aacctgtatc accacacctt gcatttaaaa tggcaaatca
    55501 aatttttcta aataagcagt gtgtattaga taaggtaaaa gttttagata aggaatttga
    55561 ttttatctta attgagggtg ctgggggaat tgccgtacca atatatgaag gtacagatga
    55621 tttctacatg actaaagatc taatcaatga ttgtgcagat tgtgtcatca gtgtgttgcc
    55681 atcaaaatta ggtgctatta gcgatgccat tgttcaccaa gattatgtta atcagaatgt
    55741 atcggcgagt aattttttaa taatgaatcg ctatacaggc agctatattg aaaaagacaa
    55801 tcaaattacg attggaaaat taacaaataa aacagtctat acatttgaag aacatgccac
    55861 gtatgaaaat ttctcagaag catttttaaa acaattaata ggagttaaaa atgaattaca
    55921 cacaacaact taaacaaaaa gactcagaat atgtttggca tccatttaca caaatgggtg
    55981 tatatagcaa agaagaagca atcatcattg aaaaaggaaa gggtagttac ctttacgata
    56041 cgaatggcaa taaatattta gatggttatg catcgttgtg ggtcaatgtg catggtcata
    56101 ataacaaata cttgaataag gtaattaaaa agcaactcaa taaaattgcc cattctacgc
    56161 tgctaggatc atcaaatatt ccgtcaatag aacttgcgga aaaattaatc gaaatcacgc
    56221 caagtaatct aagaaaagta ttttattctg atacaggcag tgcgtctgtt gaaatcgcaa
    56281 taaagatggc atatcagtat tggaaaaata ttgatagaga aaaatatgcc aagaaaaaca
    56341 agtttataac gctaaatcac ggttatcatg gggatacgat tggtgcggta agtgttggtg
    56401 gtatcaagac ctttcataaa atatttaaag acttaatatt tgaaaatatt caagtagaaa
    56461 gcccatcttt ctatcgcagt aattacgata ctgaaaatga aatgatgaca gctattttaa
    56521 cgaatataga gcaaattcta attgaaagaa atgatgaaat cgcagggttt atattggaac
    56581 cgttgattca aggtgcgaca ggcttgtttg ttcatcctaa aggctttttg aaagaagtcg
    56641 agaaattgtg caaaaaatac gatgtcttat taatttgtga tgaggtagca gttggttttg
    56701 ggagaactgg aaagatgttt gcatgcaatc atgaagatgt tcaaccggat attatgtgtt
    56761 taggtaaggc gattacaggt ggctacttac cacttgcagc tacattgaca tctcaaaaaa
    56821 tatacaatgc atttttaagt gattcgcatg gtgtgaatac ctttttccat ggtcatacat
    56881 acaccggaaa tcaaatcgtt tgtacggttg cattagaaaa tataagactt tatgaaaaat
    56941 gtaagttatt gtcacaaatt gaaacgacat catcaacact tgagaaacag ttacatgcgc
    57001 tgaagcgtca tcgaaatgtt ggttatgtaa gaggacgagg cttaatgttt ggagttgaat
    57061 tagttacaga taaagatagt aaaacgccgt tagaaattga aaaagtagaa cgtattgtac
    57121 gtaattgtaa agaaaacggg ctaatgatta gaaatttaga aaatgtcatt acgtttgtgc
    57181 cagtgttaag tatgtcaaat aaagaagtaa aaacgatggt acgtattttt aaaaaggcag
    57241 tacataacat tttagatagg aagtgttaat atgaatttgg ctaaacgcat attacaaggg
    57301 gaacaattaa caaaagagac tgtattgaaa atttatgagg atactaatat tgatacctta
    57361 gatttattaa atgaggcgta tattttaaga aaacattatt tcggtaaaaa agtaaaatta
    57421 aacatgattt taaatgctaa aagtggtata tgtcctgaga attgtgggta ttgtggacaa
    57481 tcacgagaaa ttaaacaaaa acagcgatat gctctaattc cagaggaaca aattattgat
    57541 ggagcaaagg tagcacatga taatcatatt ggaacatatt gtattgttat gagtggtaga
    57601 ggaccgagcg ataaagaagt tgatcatatt agtaacactg taagaacgat taaatctcaa
    57661 cacccgcaac tgaaaatatg tgcatgttta ggattaacga atgacgaaca agctaagaaa
    57721 cttaagtcag ctggtgtaga cagatataac cataatatta atacaagtga aaattaccat
    57781 gataacgtcg tgacaacgca tagttataaa gatagaacag atacgataga actaatgaaa
    57841 gcgaataata tatcaccatg ttctggcgtg atttgtggta tgggagaatc taatcaagat
    57901 attgttgata tggcatttgc tttgaaagaa atggatgccg acagtattcc gattaatttt
    57961 ttgcatccaa tcaaaggtac aaagtttgga agcatggatg atttaacacc aatgaaatgt
    58021 ttaagaatcg tagcattatt ccgattaatc aatcctacga aagaaattcg tattgctgga
    58081 ggaagagagg tcaacttacg ttcgttacag ccattagcat taaaagcggc gaattcaata
    58141 tttgtcggtg attatctaat tactggtggg caaccgaacc aattagacta cgatatgatt
    58201 aatgatttgg gctttgaaat tgattatgac acttgtgaaa ataaggaaaa caagaatgat
    58261 gtttcaagag caaattgatt tattacaaca gaaagggtta tatcggtcgc ttaaatcggt
    58321 agggtatata gatagacagt atattgaagt agaaaataag cgatgtacga actatacatc
    58381 taatgattat ttaggattag gtcaaatagc gtttgataag gatgatttcg aaagttttat
    58441 gcggaagtat agttatcact tatcaagttc aagattgatt agtggaagtt cgacagttta
    58501 tgaagagatt gaatcaatgt tagcaggctg gctcggatat agtgcatgta ctatcttaaa
    58561 tagtggttat gatgctaatt tggcgttatt taatattttc aaaaatacaa attgtgtcgt
    58621 attttcagat caagaaaatc atgcgagtat tattgacggt attaagctaa gtggtttaga
    58681 aaaagtgata tataagcatt tagatattgc tgatttagaa aaaaggttag cggaataccc
    58741 taatcaaaat atacaaaaaa taatcatatc tgatagtgta ttttcaacga atggcgacgt
    58801 tgtggatatt ggtcaattag tctcattaaa gcataaatat aacgcaacac tgatactcga
    58861 tgtttcacat agttttggaa tagagaatta ctcgaattat caaggcatag atatactcac
    58921 ttctagttta tctaaagcat gtggtgcata cgggggtgtg attttaagtt caaatgatgt
    58981 gaaggatatg ttaattaatc acggtagacc actcatctac tcaagtagtt tgccaattta
    59041 taatttgtat tttataaaaa gaaatattga aaagttaata aatgctgatg atagacgcac
    59101 taaattaaat agcttgagta aatattttaa ccaaaagtta aaagcactca atgttaatta
    59161 taatagttca aactcgccga ttaagtttat tgagtttgat gacatagaag cggctgagaa
    59221 cattcatcaa acattattaa agcatcatgt gtttacaagt tatttaaggt atccaactgt
    59281 gactaagcca atgttaagaa tatcattatc gtattttcat actgaacaag atatagatag
    59341 gttgtttgaa attttgcatc aagaagattg aggtgatcgt catgtatagt ataaaaatgc
    59401 gttctagcaa tcaagatatt catattagtg gtgctgaaac gatttgtgag tttgacaaaa
    59461 tagaacagac ggtacaaagg ttttataata aaggtttttt tcatgaaaat gggcaaccgg
    59521 attttctcaa tataaaaata caaaaaatta tggaaccgat taaacaaata aaagcattac
    59581 aaattattga ggatgataaa gcgaatctcc aacatttaac acaaaaatgt ggtgtcactg
    59641 aacaagcgct aaatcaaggg atgacatata ttaaaaatga aacggtttat acaggagcta
    59701 ttattctatc tgctatatct ggtaaacgtt tagattcatt tggacatagg gggattaggg
    59761 caacacattt ttcgtttgaa gatataaata ataaaggtga tcttaatgaa agagtgactg
    59821 atgccttagc aattgctagt tgtatcaatg cgcatccgta tgtcaaagga gaactttgcg
    59881 tgtccgatga cttaacgtat acgacaggtt attttgcctc tgctaaaatt ggttaccatc
    59941 gattatttga tattaaacca gttaatacga gatatggagg cagaataata tttgtggacg
    60001 atcgtattga tttaaatcat tacatattat ttttagaaag cacaccgaag caagttgttt
    60061 atgaaacggt ataggggttt tagtatgaca tcaaaagata ttactcaaat tagtgtcatt
    60121 gctgcgattt taaccatttt ggcagttttg aaaataccat ccattatacc aggattagat
    60181 tttcaattat ctgcaccagc agcattattg atattagctt tctttggaat taaaaagtac
    60241 tttttaggtg gattattatc tagcctatta ttactagtat ttggcgtatt taatccgatt
    60301 aatgtgatta tttctattat atttagagtt atagctattg cagttgttta tttattgaaa
    60361 ataaatgtac tatcattagt tttagcaagt gtattaggca gtttggtatc aagattgata
    60421 ctatcggtca ttttaaattt acctgtgtgg gtcgtgttat taaacgcgat tccaggcgta
    60481 atattcactt taattgtagc tattccttta tatctcatat tgagaaaaag aatggcagtc
    60541 ttactaagat aataaatcaa aacacggtcg tcacaattac tgttggcgac cgtgttttac
    60601 tagttattta ttgttttcag tttcttttgt atctaacaat ttcactttgt gattttccca
    60661 atcaatttca tatgttgatt taaatgttct agttttaaag tttttataat tcgcgcctgc
    60721 ccagtagaag ccattccaac gaatttggta taaatccatt tcacgttgat aagttactgt
    60781 aattttagat tttttagcgc catcttgtct gtgtgatagt acgcttaaaa attctggatt
    60841 gaagttactt ctagataata atggcatttg gtgttgcgct atgaagtttt ggccagcgta
    60901 tgcactgctt tgtctgccag ctaagaagag ttcattacca tatgttgggt ggaagctatc
    60961 tcgtccataa ggtccccaac cattattcat aattttatgt gcttctacac cccaaccgac
    61021 atttttataa tttgtgttgc gacttaatgt tgttctgtaa ctttcttgtt tataattaat
    61081 tgtttcagaa aaagcagtgt ttccgttaag tccgccagat aaaccattag agatactgat
    61141 gtcaccacca aatgtatagc ctaaagtgtt ttgaacttga aattcttcat tttgattttt
    61201 aggtgcatag tctactacat taaccgaatc attagattgt gaactgatag acacattgta
    61261 ttttgcaccc caatatattt ttgaaaagtc gtagtcttta ggatttggtc tttcataacc
    61321 tgagttaata ttacctgcag ctttaagcac taaagtatct ttatcataac ttttatcttt
    61381 gatgaaattg aatgttaaaa tctgtgaaat tttgaattta tctgaatctg ctgtagctgt
    61441 agttttgtat aaagtaactt tatcatctac ttttttgacg ctaactggtg tgattttgcc
    61501 ttcagcattt gcagtattag aaagtaataa taatgccata gacgtagcaa cggatgattt
    61561 gactaattta ttcatgttca tatcaattct gtcctttcac tttgatttcg tgagtcttcc
    61621 aattgacttc atatttcaca gtgtagtttc tatttttaaa tgcattatgg actctgtgac
    61681 catctaaata actgttgcca taatgtgttg atcttttaat ggcatgagtg acatccatat
    61741 ttcttccata agtgatttca aattcgctcg tgtcgcttga acctttttcg tgagatactg
    61801 ttgcgataaa tgaagggtta aatccacttt gtacaagagg tggtaactcg ctgtctggaa
    61861 cgaaataatc tctaggatct ttactatgag gtttgtagcc tacaaataaa tcgctatcaa
    61921 atgctgattt ttgacctgat tcagtggcga atgaattcgc tttgacgccc cataaaacac
    61981 tttttgagtt ttgttgttct acttcactta cataattttg ttgtgtatag ctaatcgatt
    62041 tagaatagtt aaatgatcca ttaccaccaa gtgatggggc tgattggaaa ttaccaccaa
    62101 tattgtatcc taatgtctga cttacgtttg tagattcaat tttatttttc ggtaaataat
    62161 tgattaaaga aacatattta tcatttgttt tcaaaccaat attatattgg aa
//
LOCUS       DATTQF010000003        52420 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.003, whole genome shotgun sequence.
ACCESSION   DATTQF010000003 DATTQF010000000
VERSION     DATTQF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.003"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(111..473)
                     /locus_tag="VB241_000117"
     CDS             complement(111..473)
                     /locus_tag="VB241_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP1243453.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVVHQKN"
     gene            complement(470..640)
                     /gene="mazE"
                     /locus_tag="VB241_000118"
     CDS             complement(470..640)
                     /gene="mazE"
                     /locus_tag="VB241_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HEP1243454.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(725..1873)
                     /gene="alr"
                     /locus_tag="VB241_000119"
     CDS             complement(725..1873)
                     /gene="alr"
                     /locus_tag="VB241_000119"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP1243455.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYHRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(1939..2298)
                     /locus_tag="VB241_000120"
     CDS             complement(1939..2298)
                     /locus_tag="VB241_000120"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP1243456.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(2302..2793)
                     /locus_tag="VB241_000121"
     CDS             complement(2302..2793)
                     /locus_tag="VB241_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEP1243457.1"
                     /translation="MSDYKFMSPHAKKVMRLSAVLFWIPIAFVLLIAFNILNWLFWEI
                     LDNYISIVSSVIIILLVAIFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHIENNK
                     NEE"
     gene            complement(2786..4363)
                     /locus_tag="VB241_000122"
     CDS             complement(2786..4363)
                     /locus_tag="VB241_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEP1243458.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFVIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQMIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSST
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIAAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKMKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITVVIIGLFVIKGYLYVKHAGFKMTDEQIT
                     IQNFVLLKRNIYYFKAQHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYASS
                     KDVQILKSWYLRGENDE"
     gene            complement(4356..4835)
                     /locus_tag="VB241_000123"
     CDS             complement(4356..4835)
                     /locus_tag="VB241_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243459.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPQYLIYILFVLCTLTVLKLIIKPLWQYHCRFYQVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(5044..5604)
                     /locus_tag="VB241_000124"
     CDS             complement(5044..5604)
                     /locus_tag="VB241_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEP1243460.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RVKHHVKFDNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(5624..7651)
                     /gene="kdpB"
                     /locus_tag="VB241_000125"
     CDS             complement(5624..7651)
                     /gene="kdpB"
                     /locus_tag="VB241_000125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEP1243461.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMLL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMMEKLIVAA
                     YMSSIYDDTLEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESICMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFAELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(7670..9346)
                     /gene="kdpA"
                     /locus_tag="VB241_000126"
     CDS             complement(7670..9346)
                     /gene="kdpA"
                     /locus_tag="VB241_000126"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HEP1243462.1"
                     /translation="MEIILFLTMMVMIAYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGVITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESGVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIHGANMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMISSSLVN
                     KKSYHQDKHTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(9370..9480)
                     /gene="kdpF"
                     /locus_tag="VB241_000127"
     CDS             complement(9370..9480)
                     /gene="kdpF"
                     /locus_tag="VB241_000127"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEP1243463.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            9618..12275
                     /locus_tag="VB241_000128"
     CDS             9618..12275
                     /locus_tag="VB241_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEP1243464.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKLGYIEPHQRDETNALAEQLPKIATNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KSRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KRRDFFNKPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSYIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYAITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNKTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISIEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDI"
     gene            12275..12970
                     /locus_tag="VB241_000129"
     CDS             12275..12970
                     /locus_tag="VB241_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1243465.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTAENATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(13326..14846)
                     /locus_tag="VB241_000130"
     CDS             complement(13326..14846)
                     /locus_tag="VB241_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1243466.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELIEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(15363..16721)
                     /locus_tag="VB241_000131"
     CDS             complement(15363..16721)
                     /locus_tag="VB241_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP1243467.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FEGENVDGHRFVSKALQDGAGAAFYQKGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCCVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIGVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            complement(16736..17806)
                     /locus_tag="VB241_000132"
     CDS             complement(16736..17806)
                     /locus_tag="VB241_000132"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP1243468.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITTEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSIGISKCSNEVELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            18124..19326
                     /locus_tag="VB241_000133"
     CDS             18124..19326
                     /locus_tag="VB241_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEP1243469.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            complement(19487..20170)
                     /locus_tag="VB241_000134"
                     /pseudo
     CDS             complement(19487..20170)
                     /locus_tag="VB241_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20582..20719)
                     /locus_tag="VB241_000135"
     CDS             complement(20582..20719)
                     /locus_tag="VB241_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243470.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(20847..21056)
                     /locus_tag="VB241_000136"
     CDS             complement(20847..21056)
                     /locus_tag="VB241_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP1243471.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVIINIIDGQVT
                     VAYETPANLNSIEKEIYDEGYKIVF"
     gene            complement(21068..21361)
                     /gene="csoR"
                     /locus_tag="VB241_000137"
     CDS             complement(21068..21361)
                     /gene="csoR"
                     /locus_tag="VB241_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HEP1243472.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            21527..23011
                     /gene="cls"
                     /locus_tag="VB241_000138"
     CDS             21527..23011
                     /gene="cls"
                     /locus_tag="VB241_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP1243473.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKVYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLHPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            23039..23686
                     /locus_tag="VB241_000139"
     CDS             23039..23686
                     /locus_tag="VB241_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP1243474.1"
                     /translation="MQQSDIISAAKKYMESIHQNDFTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEQMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFLTEWNYHD"
     gene            23843..24142
                     /locus_tag="VB241_000140"
     CDS             23843..24142
                     /locus_tag="VB241_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243475.1"
                     /translation="MMWRCGVMGGIARGIDAMPLQFYDMSLGRCNVYYAVVTLLFHTV
                     TCICELLWKCCDIYCYTDESLCQDDIAMKNSNSVINHFLHGEISLILFQIKTAVG"
     gene            complement(24299..25171)
                     /gene="yidC"
                     /locus_tag="VB241_000141"
     CDS             complement(24299..25171)
                     /gene="yidC"
                     /locus_tag="VB241_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP1243476.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(25257..25898)
                     /locus_tag="VB241_000142"
     CDS             complement(25257..25898)
                     /locus_tag="VB241_000142"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEP1243477.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGFSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
                     NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            complement(25900..26691)
                     /gene="thiM"
                     /locus_tag="VB241_000143"
     CDS             complement(25900..26691)
                     /gene="thiM"
                     /locus_tag="VB241_000143"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEP1243478.1"
                     /translation="MNYLNNIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYAIYKTAIVIT
                     GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            complement(26675..27505)
                     /gene="thiD"
                     /locus_tag="VB241_000144"
     CDS             complement(26675..27505)
                     /gene="thiD"
                     /locus_tag="VB241_000144"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP1243479.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNNTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDADIAKDYLFTKEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(27498..28187)
                     /gene="tenA"
                     /locus_tag="VB241_000145"
     CDS             complement(27498..28187)
                     /gene="tenA"
                     /locus_tag="VB241_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEP1243480.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(28267..28369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(28712..29407)
                     /gene="sceD"
                     /locus_tag="VB241_000146"
     CDS             complement(28712..29407)
                     /gene="sceD"
                     /locus_tag="VB241_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HEP1243481.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAQASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSADQSNQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNDHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     gene            complement(29796..30191)
                     /locus_tag="VB241_000147"
     CDS             complement(29796..30191)
                     /locus_tag="VB241_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1243482.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIERYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            30385..30825
                     /locus_tag="VB241_000148"
     CDS             30385..30825
                     /locus_tag="VB241_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HEP1243483.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            complement(30882..31322)
                     /gene="fabZ"
                     /locus_tag="VB241_000149"
     CDS             complement(30882..31322)
                     /gene="fabZ"
                     /locus_tag="VB241_000149"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP1243484.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(31356..32621)
                     /gene="murA"
                     /locus_tag="VB241_000150"
     CDS             complement(31356..32621)
                     /gene="murA"
                     /locus_tag="VB241_000150"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP1243485.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LEYQEDGIRVRAEGDLQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            complement(32732..32965)
                     /locus_tag="VB241_000151"
     CDS             complement(32732..32965)
                     /locus_tag="VB241_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP1243486.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            complement(33844..34248)
                     /locus_tag="VB241_000152"
     CDS             complement(33844..34248)
                     /locus_tag="VB241_000152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP1243487.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(34268..35680)
                     /gene="atpD"
                     /locus_tag="VB241_000153"
     CDS             complement(34268..35680)
                     /gene="atpD"
                     /locus_tag="VB241_000153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP1243488.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(35702..36568)
                     /locus_tag="VB241_000154"
     CDS             complement(35702..36568)
                     /locus_tag="VB241_000154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP1243489.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(36599..38107)
                     /locus_tag="VB241_000155"
     CDS             complement(36599..38107)
                     /locus_tag="VB241_000155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP1243490.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(38129..38668)
                     /locus_tag="VB241_000156"
     CDS             complement(38129..38668)
                     /locus_tag="VB241_000156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP1243491.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELVKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(38668..39189)
                     /locus_tag="VB241_000157"
     CDS             complement(38668..39189)
                     /locus_tag="VB241_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP1243492.1"
                     /translation="MTETANLFFLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(39389..39601)
                     /gene="atpE"
                     /locus_tag="VB241_000158"
     CDS             complement(39389..39601)
                     /gene="atpE"
                     /locus_tag="VB241_000158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP1243493.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(39644..40372)
                     /gene="atpB"
                     /locus_tag="VB241_000159"
     CDS             complement(39644..40372)
                     /gene="atpB"
                     /locus_tag="VB241_000159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP1243494.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(40393..40845)
                     /locus_tag="VB241_000160"
     CDS             complement(40393..40845)
                     /locus_tag="VB241_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEP1243495.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQY
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKSHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(40909..42039)
                     /gene="wecB"
                     /locus_tag="VB241_000161"
     CDS             complement(40909..42039)
                     /gene="wecB"
                     /locus_tag="VB241_000161"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEP1243496.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLKQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLNE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENLGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVRDVAHKILGGHDRIELIEPLDVIDFHNFAKQSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVDAGTLKVVGTHEQDVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDFN"
     gene            complement(42063..42692)
                     /gene="upp"
                     /locus_tag="VB241_000162"
     CDS             complement(42063..42692)
                     /gene="upp"
                     /locus_tag="VB241_000162"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP1243497.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(42720..43958)
                     /locus_tag="VB241_000163"
     CDS             complement(42720..43958)
                     /locus_tag="VB241_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP1243498.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(43985..44509)
                     /locus_tag="VB241_000164"
     CDS             complement(43985..44509)
                     /locus_tag="VB241_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP1243499.1"
                     /translation="MKDLTMLLHELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQFNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(44616..45035)
                     /locus_tag="VB241_000165"
     CDS             complement(44616..45035)
                     /locus_tag="VB241_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEP1243500.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(45032..46078)
                     /locus_tag="VB241_000166"
     CDS             complement(45032..46078)
                     /locus_tag="VB241_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP1243501.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(46162..46998)
                     /gene="prmC"
                     /locus_tag="VB241_000167"
     CDS             complement(46162..46998)
                     /gene="prmC"
                     /locus_tag="VB241_000167"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP1243502.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(46985..48061)
                     /gene="prfA"
                     /locus_tag="VB241_000168"
     CDS             complement(46985..48061)
                     /gene="prfA"
                     /locus_tag="VB241_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP1243503.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(48062..48661)
                     /locus_tag="VB241_000169"
     CDS             complement(48062..48661)
                     /locus_tag="VB241_000169"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP1243504.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(49008..49262)
                     /locus_tag="VB241_000170"
     CDS             complement(49008..49262)
                     /locus_tag="VB241_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP1243505.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(49380..50696)
                     /gene="rho"
                     /locus_tag="VB241_000171"
     CDS             complement(49380..50696)
                     /gene="rho"
                     /locus_tag="VB241_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEP1243506.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(50943..52370)
                     /locus_tag="VB241_000172"
     CDS             complement(50943..52370)
                     /locus_tag="VB241_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEP1243507.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT        16346 a         9924 c         7824 g        18326 t
ORIGIN      
        1 ttataagaaa tttcttcgta cagaatttag aaatagttat aattattata aagttattgt
       61 cttttatatc aatattttat ttatctctga aaaatacata taatagacgc ctaatttttc
      121 tgatgaacta ctgcattcag ccctaaacta atcattagtg cattatctac ttctttcatt
      181 ttatcatcgg ataagtatgt cagtttttct ttcaatcgtt ttttatcaag tgtacgaatt
      241 tgctctaata atataactga gtctttatcc aatttatact ttttcttttc aatctctaca
      301 tgtgtcggta ttttcgcttt gttaatccta ccagttattg ccgcaacaat aactgtagga
      361 ctatatttat taccagtatc attttgaatt atgactacag gtctgactcc cccttgttca
      421 gatccctgta ctggtgataa atctgctaaa taaacatctc ctcgtctaat cattcattcg
      481 ttgaattaga agataaatat gtttcgttgc aatcgcatgc ttcacactct atcggaaaag
      541 cttcgttcgc tagggagaga tttaaatcag ccatttgtga atatccttct tttaaagatt
      601 gttctaagct atgacttcta ttttgactaa aagataacat atgagaacct ccaattgatt
      661 ccaaaagtta ttaaatgacc tttaattact ctaatgataa caaattttat atgcactgac
      721 catattattt taacaattca tttgttacaa aacgttgatc accatcatga tagattcgcg
      781 gcaaacgtct cgacaagtta caaagcactt cataattaat agtatgttgc ttttcagctg
      841 ccacctctac cgactgtgga ctttctctat gattatctat taaaatcacc gaatctccag
      901 ctttaacttg atctggcact tttacaattg tctgatccat acatacgcga ccaataactt
      961 cgcattgatg accatttaca tttacaaagc taccttgcat aatgcgtaaa tagccatctg
     1021 catatccaat aggtaacaat gctattgttg ttggatcagt agctgtataa gttgcaccat
     1081 aacttacaga ctcacccgct tgtagcgtct ttgtttgaac tacattagca attaattgca
     1141 cacttggttt aaggtgtact ttaacttttt gctgtacata ctctgatgga taatatccat
     1201 aaagggaaat tcctggtctt attgcattac agaattggca atccattaat agagaacctg
     1261 ctgagttctg acaatgtata tattcaggtt taattgcttc attgaccata tctttaaaac
     1321 ggtgatattg ttcagttgtc atatctcctg gttcgtcagc acaggcaaag tgtgtaaaca
     1381 cgccttcaaa tacaagttgc tcatattgtt gaatgatttc aatcacttct tgatacgttt
     1441 tagtatcttt aatacctaaa cgtcccattc ctgtatctaa tttaatatgc aaccataact
     1501 ttttctcttg ctcaccagaa atgtttttaa ttgcttcttt caaccactgt ttagacggaa
     1561 ccgttaaggc aactcggtgt tgtatcgctt tatcaatatc tttagctggt aacacaccta
     1621 agactaaaat tttagcagta atcccatgca ttctaagttc tatcgcttca tctaacgttg
     1681 ctacagcaaa aaatgtggca ccattttcca ttaaatgacg tgctacttta acactaccta
     1741 gtccataggc attggcttta acgacagcca tcactgtttt atttggatgc aatgtactga
     1801 atactttgaa atttgatgca acagcgttta aatctacatt catatacgca gatctataat
     1861 atttatctga catattactt cctcctgtaa ttcccacacg ttttaaaact agattttaat
     1921 tatcattgta taacaaattt aaaatgctga cttttctaaa acaacttggc tcatcgcata
     1981 atgctcagtg tgtgagatac taacatgtac gatgaaccct tcgtaatcga tctttggttt
     2041 gccaagttcg tcgttgtaac aatctatatc attaaatgcg acatgttttc ctaagcctgt
     2101 gcctaatgct ttactgaacg cttcttttgt agcaaacctg ccagctaaaa attcaatttt
     2161 acgttgctca tgtgtgaaat tgttgaattt gtgctgttca tttttagtta aaatccgctc
     2221 aaccaatttt ggttgcttac tatataacac ttttattcga tctatttcga ttaaatctac
     2281 accaattcca tgtatcattg attattcctc atttttattg ttttctatat gtctctcgtt
     2341 atttaaccag cgtttcaacc tacgtgcttc acttctatct attaattcaa tttcagcttg
     2401 accacccgct gtataaagtg tcaaagttgc taatcgatag cgtctcatta taggaccaac
     2461 atcgatatca atattttgaa tacgaaaata tggtattact ttttcatcca aaaataaaat
     2521 gccgtttcgt acacgcaaat ggtgtttttc aaatgcatat ctgcagtgct tatatcgata
     2581 gactggcgct atgacaagcg tgaaaatagc aacaagtaat attataatca cactactaac
     2641 aatagatata tagttatcta atatctccca aaatagccag ttcaaaatat taaatgctat
     2701 taaaagtaca aacgctattg gtatccaaaa cagcacagca cttaacctca tcactttttt
     2761 agcatgtggt gacataaact tataatcact catcattttc acctctcaaa taccacgact
     2821 tcaatatttg cacatctttg ctacttgcgt attttaagtt aatcgattgg gctcccacac
     2881 ctttagcaac aataaaacta aaattattta aattgttgcg tttaagtaat gtatgttgcc
     2941 aagtgtcaaa tcctataatg tgttgcgctt taaaataata aatatttcgt ttcaatagca
     3001 cgaaattctg gatagtaatt tgttcatctg tcattttaaa acccgcatgt ttgacataaa
     3061 gatatccttt gattacaaat aaaccaataa tgactactgt tataatcgta aataacaata
     3121 ataattgatt ccaaaagtaa cagccaattc ctgccatagc tatgacaata atactaggta
     3181 ttaaaaagtg tctgtggaaa cctgacaaag gcataccttc attgacttgc tgataagata
     3241 aatctggtac tagattctgg ataatttgat atgctttgtc tcgtttaata aacggcaata
     3301 tcggcacact acctgaatca ttgtcatcat cttcgatttt tttattttca aaatcacttg
     3361 tgatgataaa atgtattgca gtgtagccaa ataatcttcg tatatatgac tgacgttcaa
     3421 caacagcttg caatcgtttc gttggtacag tcactttcat cttgttaaag agtccatatt
     3481 caattttaag ttgctcattt tcaagcatca ccgaaaagcc ataaaatctt atcatcgtta
     3541 taatcgttcc aataatatat gccgctatta atactagtaa aatgataatt aatactgaaa
     3601 tacttacaat ttgaacccat tgactaattt catgatttag cttcgaccat gggatcaatt
     3661 ctcttacagc gccgtaaatc ggtactaaag ctgctaacgt tacaccaatg gcgccactgg
     3721 tcattgccat aaatagtgat tctttaaaat tcatctgata tataggaatg cgtttatttt
     3781 tctgatttag catactatca gtgttctgca cttcatctaa gcgaccttct gcgatgtctt
     3841 ccacattacc ttcaatgtca tgattacagt tgtcattctt ctcagtacta gacttttgcg
     3901 ccacttctgt cttcaactct gtttgcaatt gatcaatata tcgttcaaga tattcacctt
     3961 gctttttcga aataacactt aagacaatac catcacttgg cgttttgatt tgtaaatcca
     4021 cgccacctat catttgattg acgacccctt gagtcatatc aacggactga atacgtttta
     4081 tatttaattc tttacgcttt ttattaaaaa tacctgttgt taaaatgaaa taattatcct
     4141 caatccaata tcgcgtgttc ataattccga caatttgaga aatgtatgat attaaaaaga
     4201 atacaaatac aatacctatc cataaatatg attcgggatt cgtataatca aaatctttca
     4261 attgaaagat aacgaaaata aaaaagacga ctatgttttg tttgatagca ttgattatgc
     4321 cattaaaata tgaaatcgga tgtaattttt gaggttcaga catcactttc aacccctctc
     4381 aaattcgaca tagttctcgc ttcgattatt ttaacatcgt catgagacat catcggtaaa
     4441 taaatagtat gacctgcagt cataaatcca actttataca aattaagcac tttactaatt
     4501 ggattagatt taatcgacaa gtattgtaaa cgttcaattc gactcgtttc ttctttatat
     4561 attaaaaatg atgtacgata ttgtacactg agttgatcaa cttgataaaa gcgacagtga
     4621 tattgccata aaggcttaat gattaatttt aaaacagtca aagtacataa aacaaataaa
     4681 atataaatta agtactgagg ccattcaaat cttaaccata taaaataaaa aatgacatac
     4741 acagctacac tcaatataaa ttctaagcca ttcgtaatgt agtaatacaa caatgctgac
     4801 ttaggactct tagataactt agtataatct gacatatacc cctctcccca aataaaaatt
     4861 atacggattt ataatctatt tcattttatt tttatatgtt gataattata gcatatggaa
     4921 tatttcatgc taatttattc ttcctaaagg tacatctaaa aatttagtta agcagaaagt
     4981 acttgaatac ctaaaaaaga caccatgtta taattttatc aacatgatgt ctttcatcta
     5041 tattcaatct ttcatcttat caagagcaat atttagttca agcacattca cataatcatt
     5101 tgttaacaca ccacgctgct tacgatgttg aatcaagtcg gccactcttg aagtagatat
     5161 atgacgagca tcagcaatac gaggtgcttg cttcaatgca ttttcaaccg taatatgcgg
     5221 atctaagccc gacccagaac ttgttgcagc atctattgtt acatttgaat tatcaaattt
     5281 aacatgatgt ttcacgcgtg ctattaattc ggtgtttcca ttcgattcat tacttccacc
     5341 tgatgatacg ccgtttttat ataatttttc aggattcata ttataatcaa ctgcactcgg
     5401 tctcccgtga aaatatcgtg tctctgtcca gtgctgtcca atcaattttg atccaactat
     5461 acgattatca tacgtaatta aactaccatt tgcttgttga taaaaaaata tttgaccaat
     5521 taacgtgata gctaacggga ataaaaatcc acacaatacc atagttatta tcgttaaaca
     5581 aatactattt cttatcgtat tcatggtaca gactccttcc tcttcacaca aaaaattgta
     5641 caatcatatc tattaattta atgcctaaaa acgggacgat taatccacct aatccataaa
     5701 tcaacatatt atttataaaa attctatcaa tgctgtaacc ctttactttt acacctttca
     5761 tggcaattgg aattaaggca acaatgatta atgcattgaa tatcaaagct gatataattg
     5821 cagatttagg tgacgataaa tgcataatat ttagtgaagt catttcagga atcgttgaca
     5881 tcattagtgc aggtaaaatt gcaaagtatt ttgctacgtc attagccaaa ctaaatgtcg
     5941 ttaatgcacc tctcgtcatt aatagttgtt tgcctatttt tacaacctct attaactttg
     6001 taggattcga atctaaatca attagattgg ccgcctcttt agcactaatt gtccctgagt
     6061 tcatagctaa tcctatattc gcttgtgcta gcgcaggtgc atcatttgta ccatctcctg
     6121 tcatcgcaac aatatggcct ttcgcttgtt catctttgat gactttaatt ttatcttcgg
     6181 gtttacactc tgcaacaaat ctatcaacac ccgcttcttt tgcaattgta gctgctgtta
     6241 gagcattatc gcccgtacac ataactgttt caatgcccat ttttctcaat tcagcaaatc
     6301 gttctacaag accatcttta atcacatctt ttaaataaat cacgccaagc atgacattgt
     6361 tttcaatgac tattaatggt gtgccacctt tactcgatac atccatacag atagactcaa
     6421 tattaagagg aatattgcct tgttgttgtt tgacaagatt tatcatacta ttaggtgcac
     6481 ctttgaatac cgatatttca tttgtaatga ttccgctcat tctagtttca gctgtaaaag
     6541 gcttatatgt gccatcaatg tctttaggca gctcatttat atacatctgc ttcgctaatc
     6601 gcacaatact ttttccttct agcgtatcat cgtaaattga tgacatataa gcagcgacta
     6661 tcaatttttc catcatttgt tgattcactg gtaaaaattc actagcgatt cgattgccat
     6721 aagtgattgt gcctgtcttg tctaaaatca ttacatcgac atctccacat acttctacag
     6781 cacgcccact tttcgctaat acattgaatt gagtaacacg atccatgcct gcaataccaa
     6841 tcgccgataa caaaccaccg attgtcgttg gtattaaaca tactgttaac gcaatgagca
     6901 tcgcaatagg taaaattaaa tgcaggtaag atgctattgg atataatgtt acaataacga
     6961 ctaaaaatat aattgttaac gttgttaata atgtaaaaag tgcaatttca tttggtgttt
     7021 tatttctttc cgccccttca actaaggcaa tcattttatc taaaaaagat gtacccgctt
     7081 cactctcaac acgtatttct aaccaatcag atgttacaag tgtaccgcca atgactccat
     7141 caaaatcgcc acctgattct tttatcacag gtgcagactc accagtaatt gcagattcat
     7201 caacggttgc taatccattt attacaacgc catcagcagg gattgtttct ccattttcta
     7261 cccgaatatt ttgtccggct tttaactctg tggcgttcac tatccgatac gcaccatttt
     7321 cttctatcaa tcgagcagtt aaatttgatt gtgcttgtct taaactatca gcttgcgctt
     7381 ttccacgacc ttcagcaaag gcttctgaaa aattagcaaa caatatagtt attaataata
     7441 tgataaaaat tgtaatcaaa taacctcgcg atagatagct agttccaaat atgtcaggaa
     7501 aacatattaa tatcaacgtt aaaagcattc caacctcgac gacaaacatt atcggatttt
     7561 ttattaattg tttaggattc agcttataaa aactcatttt caaagcttct atcaccattg
     7621 tttgattgaa atatttattt acatgatgca ttttttccat ccctttactt tattttaaag
     7681 ttaaaaattc accaatagga ccaagtaata gtactggaat aaatgtcaaa ccacttagta
     7741 aaacgataaa tacgattagt gatacaccaa aataaggttt atcaatcgct attgtatgtt
     7801 tatcttggtg gtatgatttt ttattcacta aacttgatga aatcattaat tgcaaaataa
     7861 ttggtatata acgagaaagc aacataatga ttcctgtaga gatattccag aatgttgtat
     7921 catctttcag tccttcaaac cctgatccat tgttcgcagc agctgatgtc atttcataca
     7981 taacttgtga gataccatga aaagacggat tcgttatact ttcacttgct ccaggaatca
     8041 taaaagcaag tgctgaaaat actaaaatta aaattggatg tatgagaaag actaagacaa
     8101 tacatttcat ttcacgggcg ccaattggca tatttaaata ttctggcgtt ttaccaacca
     8161 tcaaactgca tataaacact gtcagtaaga catatatcaa taaattcatg agtcctacgc
     8221 cttcgccacc aaatacaaca tttagcatca ttaataccat tggtcctaat ccacctagag
     8281 gcgttaagct atcatgcatg ttattaacag aacccgttgt aaatgccgtc gtaataactg
     8341 taaatagtgc tgacaaacct gctccaaacc gtacctcttt accttccata ttcgctccat
     8401 gaattcctaa atttgctagt attggattac cacgatactc actccacata gttaatgtaa
     8461 gaattgctat aaaaatgaaa aacattgcga caaataatat cagcgcatga cgatgtactc
     8521 gtttaccatg tctacttaac atgcgaccaa ataagaacaa cattgacata ggaagtaaca
     8581 tcatactgcc catttctata aaattgctcc aaatatttgg attttcaaaa ggtgttgcag
     8641 aatttcctgc taaaaatcct ccaccattcg taccaagatg ttttattgat tcaagtgatg
     8701 caataggtcc aaatgcaata tgttgaatat gtccgcttaa agtccgaatc attaaattag
     8761 catgcaacgt ttgtggtaca ccttgagtca tcaataaaat actaattaaa catgataatg
     8821 gtaaaagcac tcggacaata aaccgaacaa tatcttgata aaaattacca atgatattag
     8881 tcaatccggt taaacgtctc aacatcgcta tacaaacggc gtaacctgat gcactagatg
     8941 taaacattaa atatgtcatt acaatcattt gcgttaaata tgtcacacct gattcaccgt
     9001 tatagtgttg taaattacta tttgttaaaa aagatattgc tgtattaaac gctaaatcta
     9061 tcgattggtt taaattatga tttggattta aaaaaagcca ttgctgaact attagcaata
     9121 caaatgttat aacccccata aatccattaa atgccagaaa atgtttgaca tatgttttag
     9181 ctgacatgtg ttctaaatct gtgccgataa ttttaaaaca catattttca aatctagtaa
     9241 atattaaatc tactcttgac gattgcacca atgctacgcg atatagatat ccactaaaaa
     9301 catatgcaat cataaccatc attgttagaa acaaaattat ttccatgata accctcactt
     9361 aatatatttc taaaattttt cactacgaat taaggcataa aataaataca aaactaatgc
     9421 aataactacc aataataaaa cgatgagcat tgccataacc tccttacaac acaacaacat
     9481 cgtaacaact tgtttatgag agaaacatta attttcaaac ttagttatta agaaatcatt
     9541 aagatgtgta tgcagaaata aattttatag catttaattg tgaagaatat tatgatattg
     9601 ctatcgaggt gaaggttatg tcaaacactg aatcgctaaa cataggaaaa aagcgtggat
     9661 ctttaacaat ttacatcggt tacagtccag gtgttggtaa aacatttgag atgctttcaa
     9721 atgccattga actatttcaa agtaacgttg atattaaact aggatatatt gaaccgcatc
     9781 aacgtgatga aacaaatgca ttagctgaac aattacctaa aatcgcaacc aattttacta
     9841 aacatggtag tcatcatttt caatatttag atgtcgaccg cataatcgaa gaatcgccga
     9901 caatagtact tatcgatgag ttagcacata cgaacatttc tagagatcgt catgagaaac
     9961 gatatatgga tattgaagaa attttaaatc atggtatcga tgttcatact actttgaaca
    10021 ttcaacatat tgaaagttta agtagtcaaa ttgaactgat gaccggtgta catgttaaag
    10081 aacgtgtacc cgactatttc ataatgagcg ccgatgtatt agaagtcgta gatatctcac
    10141 ctgaacaatt aattaaacgc ttaaaagctg gcaaggtata taaaaaggat aggctagatg
    10201 tagcatttag taatttcttt acgtatgccc acctaagcga actgcgtaca ttgacgttaa
    10261 gaacagttgc cgacttgatg agtgataaag aaaaagtccg acacaatcat aaaacgtcac
    10321 tcaaacctca tattgctgtg gcaattagtg ggagtattta taatgaagca gtaattaaag
    10381 aggcattcca tattgctcaa aaagaacatg caaaattcac tgctatttat atagatgtat
    10441 tcgaaaaaag caggcaatat aaagatagtc aaaagcaagt gcatcaacat ctcatgcttg
    10501 caaaatcttt aggagcaaaa gtaaaagtag tttatagcca aaccgttgca ttaggattag
    10561 acgaatggtg taaaaatcaa gatgtaacca aattaattat cggacaacat attagaaata
    10621 agcggcgaga ctttttcaat aaacctttaa ttgaccattt aatgtccttt gaacatagct
    10681 ataaaatcga aatcgttcca atcaaacaaa tacctgttga attgaaaatg aacaaatcac
    10741 cctatcgtcc taaaggcaaa cggttcgcca tagatatgtt aaaaatgatt ctgattcaaa
    10801 taatttgtgt aatgatggga ctgtggattt atcaacttga taagcatgag tctagtacga
    10861 ttattttaat gatttttctc atcggcatca ttttgttatc catttggacg cggtcctaca
    10921 tcattggctt tttagcagca attattaacg tatttgtttt taattatttt tttacggaac
    10981 ctagatatac atttgaagta taccgctttg actatcctat tacatttatc gtcagcattt
    11041 taacaagtat tttaacgagt gctttattaa aacaaattaa attccaatac gccattacta
    11101 aaaagcaact ttatcgtaca gatttattat ttcaatttaa tgattcgatt aaacaaactt
    11161 atacagttga aaacttacta ataaatgctg gataccaaat taatcaatta ttgcaacaat
    11221 caattactat atacgttatc aatcaatcca aagtaattaa aacaatacca ttgcaaaacc
    11281 atatcgataa taagactcaa cagcatgaac aagcattaag ctgggtaatt aaaaatgaaa
    11341 gacaagctgg tgcaacaaca gatacatttc caggcattaa taaatggttg ataccaattg
    11401 gcacatctcc gattaaaggc attctagcca ttgattacca aagttctcaa gtgattaatc
    11461 catatgatgc atcaatatta gaatcaatgt taaatgaact ttctctcgcc gttgaaaatg
    11521 tgacgttgct taaacaaaca agagaatcca tgttacaggc tgaacgtcaa ctgacacatt
    11581 caaatttttt aagatcaatt tcgcatgaca tacgtacacc gttaacaaca attatgggaa
    11641 atttagatat tttagtgtca catagtaaag atatgtctat catagaaaag gaacaattac
    11701 ttgtgcatag ttttcaagaa agtcagtatt tatatctatt agtgacaaat attttatcat
    11761 taacaaaatt acagtcatca aatgttcaga taaaattgca accttatctc gttagtgaat
    11821 tagtagaaga aatcgatatg attttagaac gtcgccattt aaaaaagcgt ataactgtat
    11881 catcctcagt agatttacag tttatacaca ttgacagtaa gttgatttta caagctttat
    11941 tcaatttgat tgaaaatgca gttaaacaca cttctactga caccaaaatc aatttatcta
    12001 ttcgttatgc tagcgatgaa caaatcgaat ttgctgttat agacgaagga cctggaatta
    12061 gtatagaaga acaacaaaag atattcgagc ctttttacac agggtcaaat aaatatttta
    12121 aagataacca aaaagaaagc atgggactag gcttatattt agtacagact atcctacaca
    12181 aacatcaatc aaacctacaa tataaaccga accaaccaca tggcagtata ttctatttca
    12241 atatagatac agactttaat gaaggagaca tataatgcaa tctaaaatat tgataattga
    12301 agatgatcac gcaatcacac atttacttga tgttgcatta actttagatt attacaatgt
    12361 aactacagcc gaaaatgcca cacaagcaca ctttaaaatt caaattgata aaccagatgt
    12421 cattttatta gatttaggtt taccagataa agatggatta tgtttgattt cagaaatcag
    12481 gcaacatact gacattccta tcattgtaat aagtgcaaga caagaagaac aaacaattat
    12541 tcaagcttta gataacggtg cgaatgacta tatgactaag ccttttaatg ttgatgagct
    12601 tcgggcacga attcgagtca tcgaaagaat cgctaagtca catcaagaaa ctaacattgt
    12661 atttactaac ggtttgttaa gcatcgactt tggctccaaa tctgttgtta ttaataatca
    12721 ggaggtacat ttgacaccaa atgaatttag cttgttagaa ctattgtcaa atcataaagg
    12781 aaaagtactc acttatgaga tgatattaaa acgaatatat ggctatgtta ataagactga
    12841 aatgcctagt ttaagagtgc atatgacatc attaagacaa aaactttcac aatgtcacga
    12901 ggacgcaaaa gatattatta aaacacatcc acgcattggt tatcaaatgt tgcagtggaa
    12961 agagaaataa ttaaataaaa aagatcgctg ccattaaacg aatgattggc agtgatcttt
    13021 tttatattca acttattatt tattgcgcac tagtgtcagc aaaatgccct attgtttttt
    13081 acaagcacat gataccgaaa ttactttcat aaaatttatt tttggtcaat tattcttagt
    13141 tttagtggtt gttatattct agattcatat ttatatttaa acagaatgat ataaccgcac
    13201 acagctctca atccaattaa atatttaacc tttaaaatgt tagtattttg atatctacat
    13261 agaatcagtt attgaaaaca aaaaagagct caagacatta catctttaag ctcttaatct
    13321 ataaattatt tttgatggtc agcaaatgtg cgacctttca taggtctgct tccaccgcta
    13381 ctcttctctt tacggtcgaa tttttttgta cttttcttct tacttgagta tccttttgaa
    13441 cgtttactct tactgtcaaa tttaggatta ccacgcttac tatttctgtt acgagaacca
    13501 cttggtttac cgttacggcc tttgcgagat aatggttttt caaaagttaa ttgaacttca
    13561 acttcatcgt tcgcttctat taactcttgt aaaagtgcag caactaaatc aacatcgtta
    13621 tattcattta acaactctgt agaaatgcgt ttcaagcgtg attctgactc tttagacatc
    13681 cagttttcaa ctttttcttt gatgtcatct tcacgtgctt gaagtacttc tttacgatgt
    13741 ggtggacgaa gtgcgctcat ttttctacca ttagcatctt caatttgtct aatgtaatcc
    13801 atttcaattg gatttacaaa tgttaccgcg ataccttctt taccagcacg acccgtacga
    13861 ccaatacggt gtgtatagct ttcagtatct tgaggtatat caaagttata aacatgactc
    13921 acaccagaaa tatctagtcc tcttgctgct acatcagtag cgactaaaat attaatttgg
    13981 tcatttttaa atttctttaa tacttctaaa cgtttcgctt gtgtaatatc accatgtaag
    14041 ccttcagctt tatatccttt agaaatcaaa gcacttgtta attcatcaac acgacgtttt
    14101 gtacgtccga atacgattgc taattcaggt tgatgaacat ctaggaaatt tgtaaatgta
    14161 tcaaatttct ctaattcttt aacaattgta tagaattctt cgatttgtgg atcagacatt
    14221 tcattattca ttgtcttaat gatttttggt gatttcataa attgttgtac taaagcttgg
    14281 attgctttag gcattgtagc tgagaacaac attgtttgac gttgtactgc tggaatttta
    14341 tccataataa atctcatatc atcgatgaat cccatattca tcatttcatc agcttcatct
    14401 aaaatcaaag tatgaattcc gtccgttttt aatgtgcgac gatttaaatg gtcgataaca
    14461 cgcccaggtg ttccgactac gatttgtggg ccttttttca aggctttaat ttggcgttcg
    14521 ataggcatac caccgaatac agtaacaact tggacacctt gtccacggct aaattctctt
    14581 aattgttcag ctacctgcat tgccaattct cttgtaggtg ctaaaatcaa cgattgaacc
    14641 ccttgtttcc ctactacttt ctcaattaaa ggaataccga atgctcctgt tttacctgta
    14701 ccggtttgag cttgcccaag gatatcaatt ccttgtaacg cataagggat actgtctttt
    14761 tggataggtg tcggctcttt aaatcccatt gattcaagtg actgaaccgt attatccgaa
    14821 atccctagtt ctttaaaatt ttgcaaaata attctccttt ttacctgttt atttctcaat
    14881 atacttttgc atataagttc attaatattt catcaacttc tatatagtac caccaaaaaa
    14941 atttaatcgc aataaagcaa tttgcgaata tttattagaa tcacattaat tgattgtcat
    15001 atttttgtca aaaagctagc attagataag atgtaagcgt tacgcatcat tcgaataatc
    15061 attataaaaa aatttttata aaatacgtat atcgttaaca ttccacaaca aattttactg
    15121 aaacacatgt gaaattagac attataatac tacatctttt tgtaaaatat tatataaaca
    15181 ttcaaactac ttcctatata tagtgtgtac atagaattaa ccttgattta gtttcaaatt
    15241 tatagattta acataaaaag aaaaaggcaa ttttgtattt acacacaaaa ccacctcatc
    15301 tttgtaatct tttagtatta attcaaatca tttttataag taaaaggtcg ctcgactaat
    15361 ctctatgaaa ttaaagcatt tacaacttct tctaacttca taccacgtga gcctttaact
    15421 aatacacggt catgcgcttt taaatcgctt gttaaaactc ctatcatatc gtctttagaa
    15481 ttgaagtgtt gtgctttttc gacatgttgc tggcccgaat cataaatata cttcgcttca
    15541 ttaccaaacg tatacaacac atctatatgc ttttcttcta aataattacc tacaccgata
    15601 tgcatttctt tgctattttc acctaattct aaaacatctc ctaaaattag aatgcgacgc
    15661 cctgtcaagg tactcagtgt atcaatagct gctctcatac ttgtaggact tgcattatag
    15721 gcatcattta tcacagtaat atcattttct aatgtatgtt gctccatacg cataccagtt
    15781 aagctgacat tttttaaatt ttgatagatt gtgttatatg tcaaacctaa ttcatgacca
    15841 accgcaatgg caatcgtcgc atttttcata ttatgctttc ctaatattgg cagatcataa
    15901 tgttctttat tattaatcgt aaatgaaata cctgtagtat ctctatcatc aacacaacaa
    15961 actaatgcat tatcagtagc aacaccaata ctaatacatt ttgcattttc aacttcttta
    16021 acatgcggtt ttaataatgg ttcatcgcca tcatatataa acgtaccatt atcttttaga
    16081 cctattgtaa tttcagattt agctttagca atcccctcac gcgaacctaa atcttgcata
    16141 tgtgactcac caatattagt tatgactgca atatctggtt gagcgaggtt tgacagaaat
    16201 tcaatttcat ggaaacctga catccccatc tccaatattg atatttcagt atcattatct
    16261 aattccaaaa tagttaaagg taaaccaatt tcattattgt aattaccttg cgttttctta
    16321 actttaaatt cggtatgcaa tacactttca atcatatctt tagtcgttgt tttaccatta
    16381 gaccctgtga cggcaattac tttagggttt acatgtctca agtaagcttg tgccaattgt
    16441 tgtaatgccg ttaatgtgtc ttcaacccat ataataggcc cgcttacatt ttcatctata
    16501 ggtgtgcctt tttgataaaa agcagcccca gcaccatctt gtaatgcctt agagacaaag
    16561 cgatgaccgt caacattttc accttcaaat ggtataaata acatattttt agaaattgct
    16621 cgtgaatcaa ttgtgactcc atttatctct tgatttaaaa attgatcttc aatttcacaa
    16681 ggaatccatg attgaatttg ctttaatgta acattaatca taataacaac ctcagttagt
    16741 caattttgta tttatttttc tgtttatcct ggtgacgttc tttagcaagc tcgataagtt
    16801 ttgtaatcaa ttctggataa gataagccca tattttccca taattttgga tacatactga
    16861 aagccgtaaa tccaggcatt gcatttgttt cattaatata tatttggttg tcttctgtta
    16921 caaagaaatc cgcacggact aaaccagaac aatctgtcgc tttgaatgcc tctaatgcca
    16981 tatttctaag cgttaattga acatcttcgt ctaagtcagc tggaatttgt aattgaacct
    17041 taccatcttt atattttgat ttgtaatcgt aaaacgcgac atcttttacg acttcacctg
    17101 gccatgtcgc ttcaggatag tcatttccta aaactgctac ttcaatttca cgtgcgttaa
    17161 cgccttgttc aataacaagc ttacggtcaa attggaatgc ttctttaata ccttctttaa
    17221 gttccacttc attactacat ttactgatac ctatacttga ccctaagtta gcaggtttaa
    17281 caaagactgg gtaatttaat ttatcattta ctaattttaa aatgttatgt tcatattttt
    17341 catattcaga acgtaagaaa ctaatataag gtaactgtgg taaccctcga tgttcaaata
    17401 attgtttcat tacaagtttg tccatagaac ttgcagctga caatacacca tttcctacat
    17461 atggtacatc caatacttca aaaagccctt gaatcgtgcc atcttcacca ttaggaccat
    17521 gtaataatgg gaatactgca tcgtatggtt gtcctgaact actttctttc agtagctgtg
    17581 aaatctcaag cgcctctcca ttttctaaat gaagctcatc agtagattta atttcagttg
    17641 taatattatt ttgctttctc caatcaccat cattggtaat ataaatgata tcaacatgat
    17701 atttgtcttt atctattgca tttaatacat tttgtgctgt cagaatcgat acttcgtgtt
    17761 ctgcactttt ccctccaaaa acgatacaaa tattttcttt tgtcatttcg ttttcctcca
    17821 atgatatatc agggtttgct accttaatat aaattaatat tgatttagtc caattgttaa
    17881 atgatttatt cgcatctttg aatcttaata gtatcacatt ttaatgctgt tttgcatttt
    17941 aaaaacgagg cgttatgtgt ctgtatctat gttgattcat ttataatcag aacaatattc
    18001 ccttctattt tattcctaat tcaaattgaa atctatagct gatatcactg tgatattcta
    18061 gctaattttt aaaaagtcat gtaaaattga tataaacatt agtgagtata aaaggagttt
    18121 gcaatgaatt attcatctcg tcaacagccg gataagcatt ggcttcgcaa agtagactgg
    18181 gtattagtag ccactatagc tgttttagca attttcagtg ttctgcttat taactcggca
    18241 atgggcggtg gtcaatacag tgctaatttc ggtatcagac aaatttttta ttacatttta
    18301 ggtgcaattt ttgcaggtat catcatgttt atttcaccta aaaagattaa acattataca
    18361 tatttattgt atttcttaat ctgtctatta ttaataggat tgctcgttat tcctgagtca
    18421 cctattacac ctattatcaa tggtgccaaa agttggtaca cgtttggccc tatcagtatt
    18481 cagccatctg aattcatgaa aattatttta attttagcat tagcgcgtgt cgtttctaga
    18541 cataatcaat tcacatttaa taaatcattc caaagtgatt tgttattatt tttcaaaatt
    18601 attggtgtct cgttagtacc aagtatttta atattactgc aaaatgacct aggaactaca
    18661 ttagtattag cagcaattat tgcaggtgtg atgttagtaa gtggtataac atggcgtatc
    18721 ttagcaccta tctttattac aggtattgtt ggtgcaatga cagtcatttt aggtattcta
    18781 tatgcacccg cattaattga aaatttatta ggtgtccaac tgtatcaaat gggacgaatc
    18841 aattcatggc ttgaccccta tacatatagt agtggtgatg gctatcattt aactgaatca
    18901 cttaaagcta tcggttcagg acagttacta ggtaaaggat acaatcacgg tgaggtttat
    18961 atacctgaaa atcatactga ctttatcttt tcagtgattg gagaggaact tggttttatc
    19021 ggttctgtca tattgatctt aatattttta tttttaatct tccatctaat aagattagct
    19081 gcgaaaattg aagatcaatt taacaaaatc tttatcgttg gtttcgtcac tttacttgtg
    19141 ttccatattt tacaaaatat tggtatgaca attcagttgt taccaatcac tggtattcca
    19201 ttaccattta ttagttatgg tggtagtgcg ctatggagta tgatgactgg aataggtata
    19261 gtcttatcaa tttattatca tgaaccaaaa cgatatgtag atttatacca tccaaaaagt
    19321 aattaattta aactattttg agtttcaaat atcataactt ttcaagatga cgttatatag
    19381 tctatttgcg tcgtcgattt aaaatgtcat atatagatat tactcgataa taacaatccc
    19441 tctttgaagt gcttgagaca ttaagttctt aggcaatgta aaaaagctaa tttctattaa
    19501 ttatttgata gaaattagct tttttgatat gtattttata atgtacagct cgttgagctg
    19561 ctattttcct tatattaagt gccattaata caaaacctag ctctcgttta actttattta
    19621 ttcctcgaac tgacatacga atgaaaccca aaatagcttt cataaatcca aaaacaggct
    19681 ctacatcaat ttttcttatt ggatttcgaa tttggcttca tgcattgatg tctcaaagaa
    19741 tatgatgaac agtcatcaca ttcatatagt ttgaagtctc gtttaaaacc atatctatca
    19801 ttacggtatg catatctttt aaaacctatt cttttgttat taggacatat aaattcatca
    19861 ttaagttcgt catatttcca attttgagtg ttgaaaatgt cacttttaaa ctttctagtt
    19921 ttatcttaaa taaacatgcc atacgtaata agtggcgttt tattaaaatc atctataata
    19981 gccatatagt tttgctcact accataacct gcatcaacta caatatactc tggtaaataa
    20041 cgaagggatt ttgaatcatt gttaaaaacg gaattaatgt tctagtatct gttgggtttt
    20101 gaaataggtc ataggataaa acaaattgag aatttgtcgc tatttgtaaa ttgtatcctg
    20161 acttaagttg ggcaaagtgt cttatttttt taaagtattt aaaagtaaaa ttacatgtta
    20221 atacgtagta ttaatggcga gactcctgag ggagcagtgc cagtcgaaga caggggcccc
    20281 aacacagaag ctgacatata gtcagcttac aacaatgtgc cgattggggt ggctgagacg
    20341 gcaccctagg aaagcgaagc cattcaatac gaagtattgt ataaatagag aacagcagta
    20401 agatattttt taattgaaaa atatcttact gctgtttttt tagggattta tgtcccagcc
    20461 tctctataga gggattttgt tatatactac ttgctttcat tttagtaaat ccagtcaaaa
    20521 aagctatcat cgaatagtcc gacgatagct ttaacggtgt gtgattcaca atcacagtca
    20581 attattttaa ttcgtgactt actttattag atgctggcaa agtattaata atgtctaaac
    20641 gagatttagt ttttttaata ttcacaccta atgatgacaa tagtttgact acattttgaa
    20701 tcttttcagg acgtttcatt attatcacct cgtttggatc gctttcaata ttatcttacc
    20761 ctattcgaca tacctttact acatcattta caaaattttt aatttatttt caaaattaaa
    20821 atctatagcg cactctacat tataccctaa aatacaattt tgtatccttc atcatagatt
    20881 tctttttcaa tactattcaa atttgctggt gtctcatacg ctacagttac ctgaccgtct
    20941 attatattaa taatcacttg atgaacacct atcatatttg aaatgcgttc tgtcaattga
    21001 cttacttgtt cttctgtttc aattcccgct gtgtaaatcg tattttgatg tatcatctta
    21061 aactccttta gtctttaatc aatttttgaa aagtcactaa taactcttcc attgcctctt
    21121 cctgagcacc ttgattaact ttattcataa tacaactttt catatgttgt tctaataact
    21181 ttgtcgcaac actgtttaac gccgaacgtg tcgctcttat ttgcgtaagg acatcatcac
    21241 aatagacatc ttcttcaatc atgcgattaa tcgctctcac ttgtccttca attcgattta
    21301 aacgtgattt aagattcgtt tttatttgtt cagaatgatg tgcattatct tgttcagtca
    21361 tagcattcac tccttcattt acattcctta ctatactata tcccataaaa cacaacgtca
    21421 acacaaagtg tgtttttcaa actaaattgt catgatattt aaattgcttc atgatacaat
    21481 taataagata tactactcgt ttaagatttt cgatgggagg tttcaaatga tagagttatt
    21541 atccattgca ctcaagcatt ctaatattat tttaaattcc atatttattg gtgcatttat
    21601 tttaaactta ttattcgcct ttaccattat tttcatggaa agacgttctg ccaattctat
    21661 ctgggcttgg ttactagtct tagttttctt gcctttattc ggcttcattt tatacttact
    21721 attaggacga caaattcaac gcgaccaaat tttcaaaatt gataaggaag ataaaaaagg
    21781 attagagtta atcgttgatg agcaattagc cgctttaaaa aatgaaaact tttcaaattc
    21841 caattatcaa attgtaaaat ttaaagaaat gattcaaatg ttgttatata ataacgcagc
    21901 atttttaaca acagacaacg atttaaaagt atacacagac ggccaagaaa aatttgatga
    21961 cttaatacaa gacatccgta atgctactga ttatattcat tttcagtact atattattca
    22021 aaatgatgaa ttaggtcgta ccattttaaa tgaacttgga aaaaaagcgg aacaaggtgt
    22081 agaagttaaa attctttatg atgacatggg ttctcgtgga ctgcgtaaaa aaggcttaca
    22141 cccgtttcgc aataaaggtg gacatgctga agcatttttc ccatcaaaat tacctttaat
    22201 taacttgcgt atgaacaatc gaaaccatcg aaaaattgtt gtaatagatg gacaaattgg
    22261 atatgttggt ggttttaatg ttggtgatga gtacttaggt aaatcaaaaa aattcggcta
    22321 ttggcgagat acgcatttac gaattgtcgg ggatgcagtg aatgcattgc aattacgatt
    22381 tattctagat tggaattcac aagccacacg tgaccacatc tcctatgatg atcgttattt
    22441 cccagatgta aattctggcg gaacgattgg cgtacaaata gcttctagtg gtcctgacga
    22501 agaatgggaa cagattaaat atggttactt gaaaatgatt tcatctgcta aaaaatcgat
    22561 ttatattcaa tctccctatt tcatacctga tcaagccttt ttagattcta ttaaaattgc
    22621 ggcattaggt ggtgttgatg tcaatatcat gattcctaat aaacctgacc atccgtttgt
    22681 tttttgggct actttaaaaa atgcagcatc cttattagat gccggtgtta aagtatttca
    22741 ctacgacaat ggctttttac actcaaaaac acttgttata gatgatgaaa ttgcaagtgt
    22801 tggaacagct aatatggacc atcgcagttt cacgttgaat ttcgaagtca acgcctttat
    22861 ttatgaccaa caaattgcca aaaaattaaa acaagctttt atagatgatt tagcagtatc
    22921 ttctgaatta acaaaagcac gttatgctaa gcgaagtctt tggattaaat ttaaagaagg
    22981 tatttcacaa ttattgtcac ctatcttata aaatagaaat atgaggagtg taactttaat
    23041 gcaacaatca gacatcatta gtgcagccaa aaaatatatg gaatctattc atcaaaatga
    23101 ttttacaggc catgatattg cgcatgtata tcgtgtcact gctttagcta aatcaatcgc
    23161 tgaaaatgaa ggtgttaatg atactttagt cattgaactc gcatgtttac ttcatgatac
    23221 cgttgacgaa aaagttgtag atgctaacaa acaatatgtt gaattgaagt catttttatc
    23281 ttctttatca ctatcaaccg aagatcaaga gcacatttta tttattatta ataatatgag
    23341 ctatcgcaat ggtaaaaacg atcatgtcac tttatcttta gaaggtcaaa ttgtcaggga
    23401 tgcagatcgt cttgatgcta taggcgctat aggtgttgca cgaacatttc aatttgcagg
    23461 acactttggt gaacctatgt ggacagaaca aatgtcacta gataagatta atgatgattt
    23521 agttgaacag ttgccaccat ctgcaattaa acatttcttt gaaaaattac ttaagttaga
    23581 atctttaatg catacagata cggcgaagat gattgctaaa gaacgtcacg actttatgat
    23641 gatgtacttg aaacagtttt taacggaatg gaattatcac gactagacat tggagttgta
    23701 gtatgatgat gcgatgtaat agcgtgttgt tgtggaagct tggtgtcatg ccatgttact
    23761 ttgatgtgtt gttgtgggag cttggtgttg atgccatgct actttgatgt gctagtacca
    23821 cgatgcgtct tgatgtagtg ctatgatgtg gcgttgcggt gttatgggtg gcatagcccg
    23881 tggtattgat gccatgccgt tacaattcta tgatatgtcg ttgggacgtt gcaatgtgta
    23941 ttatgccgtt gtgacgttat tatttcacac tgttacatgt atttgtgaat tgctgtggaa
    24001 atgttgcgac atatactgct acactgatga atcattgtgt caagatgaca ttgcaatgaa
    24061 gaatagcaac tctgttatta accacttttt acatggtgaa atctcgttaa tattatttca
    24121 aataaaaaca gcagtaggat gactttcaca tttgaaatca tcttagtgct gtttctattt
    24181 atcacatatt gtataatgtg acactaagtt tcgctattga agcgaaaaat aatgtgctcc
    24241 ctataaagtt aaaattatct tcaactttta gggggcacat tatttggact tgctaaggtt
    24301 atttcttttt ctttttagac actacttgtg tgtttttgcc ttttttattg ctgccgccgt
    24361 tgtgctctct ttcatacgct tcaatgaaag gctgtacttc ttttttagcg actttttcat
    24421 aataaatgtt cgcaaagtgt gtttgaacta caaggaacgc cgcgctgact gaccagtaca
    24481 aaccaagtgc tgatgctgag cttaatgaaa tccagataat cataattggt gaaatgacca
    24541 tcatcatgta acccatttga cgttgttcgt ctggcatcgt tttacttgat acatatgctt
    24601 ggataaagta taaaacaccg gcaataattg taatccaaat atcaggacgt cctaaatcga
    24661 accataagaa gtgtggatat ttaaacaaac catctacaag ttggtcttta agtacaaagt
    24721 ataatcccat gatgattggc aattggatta gcattggtaa acaacccaac atactcttaa
    24781 tcgggttcat gtcatacttt ttatatactt gcattaattc ttggtttgca gccatttttt
    24841 cttcttgtgt acgcgcacgc ttcacttttt cttgaatttt ctcaacttct ggctttgcaa
    24901 ctttcatttt ttgacgcatc atatgactat ttttatagtt tgacaacatg aatggtaata
    24961 aaataatacg aattaccaat acaagaataa taatagctaa accataattg tcgtttaata
    25021 agttatttcc caaccaatcc aatacatttt tcattggatc tacgaatgta ttgtagaaaa
    25081 acccactacg tttttcaggt ttagaatagt cacaaccagc caaaaagacc ataataccta
    25141 aaaataatgg tagtaacgct tttttcttca tttttccacc tctatcatta tattcacata
    25201 ggatttattc tatcacatta atgagtacgt atgaaacaat aagtggaaaa atttaactaa
    25261 ttattaaaaa aatctttgaa tcgattaaca gtcttttcaa tattttcact tttagaaatg
    25321 gctgaaatga ctgaaattcc attggcacct gcttctacaa tcggcgccac gttactagta
    25381 tttataccgc caatggctac aatcggtagt tgcggattca tttctttaaa tgttgcaatc
    25441 atttctggac ctactggtgt atgcgcgtca tgcttcgacg gcgtaggata gattggtcca
    25501 acacctatat aatcaacatg agttaaatca gattttgcat actcatctaa atcactaata
    25561 ctaaatccaa taattttatc agtgaaatat tgtgctatct ctttgacttt cgcatcatct
    25621 tgaccgacat gtataccatc cgcgttaatt tcttttgcca aggatacatc atcattaacg
    25681 ataaaaggca catcatattg atgacagaga tgctgtaatt ctttagctaa tacaagttta
    25741 tcgtttcctt ttaaagctga ttcacccttt tctcgaaatt ggaaaagtgt aatccctgcc
    25801 tttaatgctg cttctaatac ttcatgaata gttcgatgac tcggaacatc ggaggttcca
    25861 caaataaaat acacatttag atacgattgg ttaaacatat tattccacct cttgaatgcg
    25921 aatccgttgt tgataggttg tttcatttaa atgatataac gtatcaagca acaatggtga
    25981 aaacgtacca ggaccaccac aattttcatt ttcagcagct acctcagcag caatattaaa
    26041 tacgcttacc gcttcaatta acgcttctat gtctggttct gtttctctaa ataaaaatcc
    26101 agcaataatg cctcctaata aacaaccagc tccagttact cgtgctaata atggagatcc
    26161 attagctaat acgattgctt tatcaccttg aacaataacg tcctctttac ctgtgattac
    26221 tattgcagtt ttatatattg cgtaagcctt tttcgctatt gtaaccgcat caagattagc
    26281 atcactatct gtacctttca tagttgctgt atcatcaatc aacgctaata tttcagatgc
    26341 attgccttta attactgata ctttcgctga tttcaataat aatttacaaa attgctttcg
    26401 atatgtagaa gcaccaacag ctacagggtc aaatacaata ggtaagcctg cctcatttgc
    26461 cgtttgagca atcgcaataa tatcttgttc attttgggct gttaaagtac cgatattgat
    26521 taatagcgct tgtgcaactt tgtaaaattc ttcagcttct tcgggagctt cactcattgc
    26581 agggctcgca ccaatactta ataaaccatt cgctgtaaaa tttttaacta catcgtttgt
    26641 ataacaaatt gttaatgggt tttcaatacg tatgttattt agataattca tcgtctaatc
    26701 cctctttctt taaatatgca aaatgattca ctggacctct accacggccg atttcaggcg
    26761 tatattgtat actcattgaa ataaactttt tagccttgtg tacagcctca aataatggtc
    26821 taccttttgc aagttctgcc gttataactg ctgaaaatgt acaccctgtt ccatgcgtat
    26881 gttttgtttt aaatcgttca ttttcaaatg tttgaacacc ttctttagta aataaataat
    26941 cttttgctat atcagcatca tttgaatgac cgcctttaat gatgacacct ttactaccaa
    27001 tctcattaat aaagatgcgg ccagcctgca taattttttc ttcactatca atggttagtc
    27061 ccgttatttc ttcagcttct ggtaaattcg gtgttactac atcagctaat ggcaataacg
    27121 tatgttgcaa gttttgcttt gtgttattat ccattaatga atcaccgctt ttcgccaaca
    27181 taacaggatc aattacatac ggaatagatt catgttgcat taaataatga cgaatcgttt
    27241 ccatagtatc tgctgtagca atcatccccg ttttaatagc atgcggtaag gtatcattga
    27301 agacactatc aagttgttca tctacccatt gatgatttaa attatgaata tgttgtacgc
    27361 ccaatgtatt ttgagcaaca atacttgtaa cgacgcccat accatataca ccacatgaat
    27421 gaaatgattt taaatcagcc attacgccgg caccacctgt tggatcagta cctgcaatgg
    27481 ttaatgctat tttaggttta atcatttact tttcctccaa attcccattg ttctagtgtc
    27541 atagccatat tgaaaaatcg acgctcatgt atgcaacttt ctaaaaatac ttgctttact
    27601 tgttccaatt ccttgtcaga cattgattcg gctaatttat tcattaatga ctcaaatacg
    27661 ttaattatgt catccatttc agtactataa aaatcaaacc attttgccgt atctttttct
    27721 ctattaagct tatgatcact ttgagagcgc ttagctaatt ctgcataaat atatggacaa
    27781 ggtgccattg cagcaattgt ataaatagca ttttcacgac tatgcgcttg gaaatacata
    27841 tgttttatgt aatggtcgcc acttggaggc caaacttttg ttttaatgat ttcttcgtat
    27901 gattcaccaa caatttgcgc taaaatatca tgcgcaagta cttcaccttc aaccataaat
    27961 tctatttgct ctactaaaaa ttttacgtca ttcatgctat tcatttttgg aattaacaaa
    28021 gcatataagt ttgtaaattc ttttaaataa gctgcatcag cttgtaaata atgacgtaat
    28081 gcatcagctt gtatattgcc taataacatt ttttgaataa agtcatcctc ataaatatca
    28141 ttaatgattg gctttgcagc ttggtacaat ttttgtgaaa attccattgt aaaaaatcct
    28201 ccctaaataa aaaaactact tccaacatga aagtagtttg atggcaatgt tgctatacta
    28261 gccccatcac ttcaataact actttcctac gttggtacta accaaatcag gtcataaggg
    28321 tctgaacagt tcatctcagc catatcatta ggctccccta gtagttcctt agtattcaat
    28381 tgcaaattaa tcttagcaaa cggtttcaac attttcaatt attgttgctc agttgggagt
    28441 gggagagaaa tgattttttc gcaaaattta tttcgtcgtc ccaccccggc aaggttgact
    28501 agaattgaaa aaagcttgtt acaagcgcat tttcgttcag tcaactactg ccaatataac
    28561 ttcgtagagc atagaacatt gatttatgtc ccagcctgta caattatata tatttgcgaa
    28621 ataaaaaaga ccggaacata tcgagaattc cccgatatat tccaatctaa aagttactta
    28681 tataactatt ggttagctat gcataaatgg cttatgcagt aacccaatgt ccagcgccac
    28741 cagtgttata tagttttact gctgcggcat cttgaacact ttcaggagca tttgctggtg
    28801 atacaccttt atatttagca ggtgctactg aatcccaagt tgattgtaag aattgatact
    28861 taccagctgc acctgatgtt ggatttacag catgaatatt gccacctgat tcacgttgag
    28921 caatttgttt tagatgatca ttcacattta ctgatgaacc ttctgatgct tttgattcag
    28981 ttggtgttgc agtaacttgt gaattgtttg atgttgatgc ttgtggttgt tgagtttgag
    29041 cattttgtgg tgcttcaact tcttgtgatt gtacttgatt agattgatca gctgatggtg
    29101 caacattatt agttgcaggt gcttgtgcac tcatgtctgc tccattagca cctgttgcat
    29161 ggtaattcca agcaaagtgt gtaccatctg attcaaagtg ataagtaaac ccttcatagt
    29221 caaatgtata attataagcg ccagcttcaa ttggtttttg atttaatgtt tgatcatttg
    29281 attgcgccat ttgcgctaaa gatgctttat ttaagtccgc ttcacttgct tgggcttcgt
    29341 gacctgcatt tcctgctacg attcctaaac ctactgctaa tgatgatgcg agtaatgttt
    29401 tcttcataat cttaaaatcc tcctacaagt aatttgtttc tctaaaagtt ttacagagac
    29461 tactgtaaca tctgtaaaat ccgtgcaggt tacaacaaaa taaaaaaata gccttgtttt
    29521 ttacacagtg attacaacgg aatcctccat aatttcagat atgattctat cgagaattgt
    29581 catgacagta aaaatgtttt taatttgcaa ttttttatgt aatcgttttg caatctatgt
    29641 ctattttcta ttagaaaaac tacacaaaaa gacgacctac acaacagtac tagtcatgta
    29701 agtcgttttt aagtgagtat atttcaagtt aattagatct ataaaaaatt taggatggat
    29761 taaatgtata ttaaggatgt ttaggataat tggatttaaa tttctaataa gtcatgatta
    29821 tctatatttt gagagtcaat atctaaaata ctatctgaga gaatttcatt attttgggat
    29881 ttaggggaca taaattttat tgtttcaaca tataattcag tgacaaagtg tgtttgtccg
    29941 tctttatcat actttcttga tctcatttga ccagttatac caaccaatgt accttgatta
    30001 gtgtatcttt ctatattaga agctaacttg ccaaatgctt tacagaataa gtaatcacag
    30061 acgatttctc cattttcatc tttataattt cggtgcgttg caacacaaaa cgttgcaatt
    30121 tttctatcct ccttttcaaa tatttgtgcg tctttcgtca gtctcccgac aattacgatt
    30181 ttatttagca tgtagtgtgt gattcacctc ctatgcctat atcttaatta ctagcttaaa
    30241 aatggtcaaa atggcaaatt ttatataatt aatagctata gcatctcgtt atttgttata
    30301 tttaaagaaa ttagtcatga aatgacctta aatcttctaa ataattcaaa aatcataaat
    30361 attaaactaa tgggatgtgg gaatatgaaa acttttaaag cagtaaggtt tcaaattgtg
    30421 aatgaacacg gcagaattat agaatatgaa ttagaagatg gcgtaataat taataaagag
    30481 gaaagtggca cgggttggct gcttgaaatt gtcatttcta atgagcatta tgaaacattt
    30541 aaggaatatc aagacaatga acagttattg gatattcgtg tcgtcatcac gcgtccagcc
    30601 aatgatccag ctttatttga atctactgtg aaaagtatta aaaattttaa aacaacaatg
    30661 tctatcgtat ttgaatgtca tatttacact ttacgtcaac agtatgctga aagtttatta
    30721 gaacaactta ttgatgatgg attatctggt gaagaattaa aaaaatcatt taatcgaatg
    30781 atgcagtcta aaccaaagtt aaaggatgaa aaattagaag agtaaaataa aaaacattca
    30841 aagattatgc caacacatta aatctttgaa tgttttttgt tttattttac atcttgaatt
    30901 gcaaatgtaa gttcacaact acaagcaagt tgaccatcga cagtagcttt agcattacct
    30961 ttaccgattg gtcctttaat tttagtgatt tctacttcca acgttaaagt atcaccaggt
    31021 actacttgac gtttaaaacg acatttatca ataccagcaa ataaagcgat tttaccttta
    31081 ttttcttcac tatttaaaat agctaccgca cctgtttgag ctaacgcttc agttattaat
    31141 acgcctggca ttaccgcata ctcaggaaaa tgcccttgaa agaatggttc gtttcctgat
    31201 acttgtttaa tagccacgca acgttgacct tcttcatatt caacaacttt atcaattaat
    31261 aaaaatggct gtctgtgagg tataatttgt ttaatttggt tataatcaaa aattgtttcc
    31321 atggttgaaa tcctccatta tattaattta ctgaattaat cgttaatacg ttcaatgtct
    31381 gcacctaatt gcttcaattt accgtgtaag tcaacatagc ctctatctag gtgcgttaat
    31441 tcagtaacgc ttgttttacc atcagcaact aatccagcta aaattaaggc tgctgctgct
    31501 cttaaatcag tcgctttaac ttgtgcacct tgcaattgac ttttaccttc aagtttagca
    31561 ctacgacctt ctacattgat attagcattc atacgtttga actctgcaac atgcataaaa
    31621 cgattttcaa atacagtttc agttactact ttatgtccgt ttgctgttaa taataatgcc
    31681 atcatttgtg actgcatatc ggttggaaaa ccaggatgcg gtaatgtttt gatgtcaaca
    31741 ggctgtaaat ctccttcagc acgtacacga ataccatctt cttgatattc caattcaacg
    31801 cccatttctt ctaatttata cactaaacta gccatatgtt ctttgattgc accacgtaca
    31861 aaaatatcgc cacgagtaat ggcaccagcg atcagtaatg tgcctgcttc aattctatct
    31921 ggaatgatag catgttctac accatgtaat gattctacac cattgattgt aattgtgtct
    31981 gtaccagcac cagtaattct accgcccatt tcattaatgt agtttgctaa atcaacaatt
    32041 tcaggttctt tagctgcatt ttcaattaaa gtcttaccct tagctaatga tgctgccata
    32101 ataatatttt gtgttgctcc tacacttgga aaatctaaat gaattgatgt accttttaat
    32161 ccatctttag cattagcata aatattacca ttttcaagat gaatttctgc gcctaaagct
    32221 tcaaaacctt taatgtgttg ctcaatcggt ctacttccaa ttgcacaacc accaggcaat
    32281 gcaacaatag catgtcctag tcttgctaaa agaggtccca taactaaaat acttgcacgc
    32341 attttactaa catattcata tggtgcctct tcatttagag tctttgttgc atcaacgaca
    32401 acagcatttt cgtccttttt gtatgtaacg tcagcattta aggttgttaa tacattattt
    32461 attgtttcta catcacttaa agctggaaca ttaactaatt tgctcggttt atcagaagct
    32521 aataaagatg ctgttaatat aggtaatact gcatttttag caccttctac tttaacttca
    32581 cccgttaatt tatttccacc tttgattact attttatcca tcgtaaatcc tccacttaga
    32641 actcctatta ctttacattt ccctatttta aaattatttt aagtcattat ttaattatat
    32701 ctagttttga ttacaaatca tagagttaga cttattttat taaatatttt acctgcgtcg
    32761 agtattgtaa caaatctaca ataaaattgc ttactgcagt gcctaataaa atagcaacaa
    32821 atatcataca cacttgtaat tgagtggcat atcctttttt aaaaaattga tctaatctaa
    32881 ttgattgtaa agcccaatag gcaatacaaa tacatacaac atgtaacact aaatggataa
    32941 ctgcatattg tccgatataa tccattttat cacctcataa gattactgta ttacattgta
    33001 catgaaaaat ttaaataatg atacaaagtt taatacccgt ttttagtaaa gtttaagcta
    33061 acttactata taattctttt tacataaata tcataactat aaagtatctt ggcatggaaa
    33121 aatgtagcta ctttcaaaaa aagaaacgtc gactgtagat agtggtgaaa ggaaattttg
    33181 attttagcaa tctattaatg attgtaaaaa gtaaaactat tcttgggagt cgaacataaa
    33241 tcgatgtcta gactctatag tgttatattt ggcagtagtt gacggggccc caacataaag
    33301 aaattggatt cccaatttct acagacaatg caagttgggg gcggggcccc aacacaaaag
    33361 ctggcggaaa gtcagcttac attaatgtgc aagttggggt gggccccaac ataaagaaat
    33421 actttttctt tagaaattag tatttcttat caggctggga cataaatcaa tgttctatgc
    33481 tctacgaagt tatattggca gtagttgact gaacgaaaat gcgcttgtaa caagcttttt
    33541 tcaattctag tcaaccttgc cggtggggcc ccaacaaaga gaaattgtat tcccaatttc
    33601 tagagacaat gtaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
    33661 tcccactccc attcctattt ttaaatacac attagctgtg tttaatgata aagaaccact
    33721 acatcataaa tctttaatgg ttctttatca attccttccc cactctcatg ttttgtatct
    33781 ttaaattaat ataattcaaa aaagggtcga agatatgaaa tcacatcttc gaccctttat
    33841 ttactatttt aattcagcca cacgcaaacg gttatttgct ctctctaaag ctctttcggc
    33901 tctatgaata tcagtattgt cgtcatcatt ttccaagtga gactctgctc ttgctttagc
    33961 taatttagct ctttcaacat caatttctct tgcagtttct gcagtctgaa caatgattga
    34021 aactttatct tttctaactt caacaaagcc atcgcttaca gcaatatatt cagttccatc
    34081 gtgaaatttc acttttacaa agcctgtttt taaagcagct acagttggaa tatgtccact
    34141 catgacacct atctcaccag ctgttgtttg cataacaacg agttcaacat tatcacgatt
    34201 gtaaacagaa ccattaggag tgacaatatc taggtttaat gtattcatta tccattcctc
    34261 ctaattgtta tacttcaaca cccatatctt tagcttttgc aataacatca tccatgctac
    34321 caactaaacg gaatgcatct tctggaatat ggtcatattt accatctaag atatctttaa
    34381 agtttgcaac tgttgtctta acaggtacat aagaaccttt ttgaccagta aattgttccg
    34441 ctacgtggaa gttttgagat aagaagaatt gaattctacg tgcgcgttca actgtttgtt
    34501 tatcttcatc agataattcg tccataccta agatagcaat gatatcttgt aattcacggt
    34561 atttttgaag tgttgattgt acatcacgag ctacttcata atgttcttga cctacaattg
    34621 atggttccaa tgctcttgat gtagacgcta atggatccac ggctggataa atacccattt
    34681 cagttaattt acgttctaag tttgtagttg catctaaatg ggcaaacgct gtcgctggcg
    34741 ctgggtcagt atagtcatcg gcaggtacga ataccgcttg aatagaagtt actgatcctt
    34801 ttgttgtaga cgtaatacgt tcttgtaatt gtcccatttc agtagcaagt gttggttggt
    34861 aacctactgc agaaggcata cgacctaata atgcagatac ctcagaacca gcttgtgtaa
    34921 atctgaaaat gttatcgatg aataataata cgtcttgacc ttgttcgtca cggaaatatt
    34981 cagccattgt taaaccagat aatgcaacac gcatacgtgc accaggtggc tcattcattt
    35041 gcccgaatac catggctgtt ttcttaatta caccactatc actcatttcg aagtataaat
    35101 cgttaccttc acgagtacgt tcacctacac cggcgaatac agaaatacca ccgtgctctt
    35161 gagcgatgtt gttaattaat tcttggatta atactgtttt acctacaccg gcaccaccga
    35221 acaatccgat tttaccacct ttaatataag gtgctagtaa atctactact ttaatacctg
    35281 tttctaaaat ttgaacttct gttgaaagtt catcgaatgc tggtgcttga cgatggatag
    35341 gatcgcggcg aacagaatca ctaatttctt ctttaaggtc aattgtttca cctagtacat
    35401 taaatacacg acctaatgtt tcatcaccaa caggtacact aatttctttg cctgtatctt
    35461 ttacatccat gcctctttgg acaccatcag ttgaatccat cgcaattgta cgaacaacgt
    35521 cgtcacctaa ttgcagcgca acttctaatg ttagttgtat tgtaccttct tctttaggca
    35581 catcaataac caaggcgtta ttaattttag gaacttcgtt atgttcaaat cgaacatcaa
    35641 ttacaggacc cataacttga gttacacggc caattcccat gctattttcc tcctttaaat
    35701 attattcaag cgctgcggaa ccaccaacaa tttcagtaat ttgttgcgta atttctgctt
    35761 gtctcgctct gttatattct aatgataagt catcaataag ttcagttgca ttatcagtgg
    35821 catttttcat cgcagtcata cgtgttgcat gctcacttgc ttttgcgtct aatattgttc
    35881 cgtaaatcaa actctcaaca tattgaggca agattacact taagatagat tctttatctg
    35941 gctcaaattc ataagaagac aaatgaccat gtcccttact agaatcctct tgagataatg
    36001 gtaatacttg tctagatgta ggcttgtttt caagaacgct gacataatga ctatagtata
    36061 tatttaattc atcaatttct tcttcactgt ataagtctat agcatggtta gctagtgctt
    36121 gaacagattt gaaagaaggt tgatcaggta cgtctacttg agaatactca atgtcataac
    36181 ctctattttt taggaaatca acaccttgtt gccctaaaac tacaatactg tattcgctac
    36241 tatcttgatg tttcgcttca atatcagtaa tcaatttttt aagcacgttt gcactatatg
    36301 cacctgctaa acctttatca ctcgtgataa ctaaatatcc acttctagta attttacgag
    36361 gtcttagcat tggatggttt gtattgctac ttgcccctgc aactgcagta attgcatctt
    36421 gcattttatc catatatggt gtgaattgtt ttgtattttt ttcagctcta cgaagttttg
    36481 aacttgatac catgttcatc gctttcgtaa tctgcttcat ttttttggtt gattttattc
    36541 gagtatctat ttctttaaga gaagccacta tctcaccacc ttatactaaa cttgtttatt
    36601 attcagattt gctaaagctt tttttgaatt cgttaattgc tgtgtcaaac ttctcagcat
    36661 ctggtaagcc accagtttct ctgatttcat ttaacagttc agtagcattt gattctgccc
    36721 agtggtttaa ctcgtcttca aaacgtgtga tatctacaac tggaatatca tctaaatatc
    36781 cttttgttaa tgcataaata atcaacactt ggtgttcaac tggtaatggt ttgttttgat
    36841 cttgttttaa gacttcaaca gtacgtttac cacgttctaa tttacttgca gtaaattcat
    36901 caaggtctga accgaattgt gcaaatgatt caagttctct atatgacgct aagtcaagac
    36961 gtaacgtacc agcaactttc ttcattgctt taatttgtgc agatccacca acacgagata
    37021 cagattgtcc ggcattaatc gctggtctta cacctgagaa gaataaatca gattgtaaga
    37081 agatttgtcc atctgtaatt gaaataacgt ttgttggtac ataagctgaa atatcaccag
    37141 cttgtgtttc gataattggt aatgcagtaa ttgaaccgcc acctaagtca tcgtttaatt
    37201 ttgctgctct ttctaataat ctactatgta agtagaatac gtcacctggg tatgcttcac
    37261 gacctggagg tctacgtaat aataatgata actcacgata agctgctgct tgtttagtta
    37321 aatcatcata tacgattaaa acatgtttac cgttaaacat gaattcttca cccattgtta
    37381 cacctgaata tggtgcaata tataataatg gagaaggttc agaagctgat gctgctacaa
    37441 caatagtgta gtctaaagcg cctgcttgtc ttaacttttc aacatttgct cttactgttg
    37501 aatctttttg accaatagca acatagatac aaatcgtacc ttgatctttt tggttcaaaa
    37561 ttgtgtcaat tgcaattgtt gttttacctg tttgacggtc accgatgatt aactcacgtt
    37621 gacctctacc aattggtact aaagcatcaa ttgctttgat acctgtttgt aatggctcat
    37681 ctactgattt acgatccatt acaccagtag cttttttctc tactggacgt gttttagttg
    37741 tgttaatcgg tccttgtcca tcaataggtt gtcctaatgg attaacaact cttccgatta
    37801 gttcttcacc tactggtact tccatgatac gaccagtacg tttaacttcg tcaccttcag
    37861 taatacctgt gtatggtcct aaaataacca cacccacgtt tgactcttca aggttttggg
    37921 ctaaaccaag tacgccgtta tggaattcta ctagctcacc agccataacg tcatttaatc
    37981 cgtgaattaa tgcaatacca tcaccaattt gtaatacagt acctacatca gttacggaca
    38041 tttctgactc ataattttca atttgtgagc gaagtaatgc actgatttct tcagctttta
    38101 tggccatcta tgtcactcct ctttataatt aattaactct tctaaatttt ctttgtaatt
    38161 ggacaagatc atttctaaca ctaccatcta atacagttgt gccgacttta actctaaatc
    38221 caccaattaa atctggatta atttttgtat ctacaataac tttagataac ttcgtttgtt
    38281 gagttactag tttgacaatc ttaactaact cttcttgact caattcatat gttgactcaa
    38341 ttgttgcaaa atcttgattg tagtgtccgt tatataagct ttggaacgcc ttgaatacgt
    38401 cagctattaa tgagatatgt ctattatctg ctaacacgta catcatattt ttaatgtatg
    38461 gattaatgtc agtaaataca ccattaatca actcacgacg ttgttctgct gtttgagttg
    38521 gattactgtc aaccattttc aattgctcaa ttttatcttt tactgcttcg tttataactg
    38581 ttaattcttc attaatagtt tctaaattat ttgtatctaa tgacacgtca aataatgctt
    38641 tagcatactt gttagctact tttaccatta tttatcgcct gcctctttta gatacttgtc
    38701 aaccaatgct ttttggtctt gttcagaaat ttcttttcta agaactttag aagcaattaa
    38761 cactgatagt tcagatactt gattattaat atctgcaatg gcacgttctt tttggctatt
    38821 gatttcactt tgtgctgttt caatcatacc gtttgcacgt acgtttgctt catgaataat
    38881 ttgttcttgc tgttgacgtg cttgaacctt agcatcttct aaaatctttt gaacttcttc
    38941 ttgtgtttct ttaagttttt gtttattttc ttcttcaagt ttctgtgcat ttaacttagc
    39001 ttgttctgcg tcatcgatat ctctgttaat atctctttca cgtttatcca ttacatcttt
    39061 caatggaccc catgcgaact ttttaagtaa cgcaagtaat acgatgaaag ttaggacctg
    39121 tacaatcaca gtaccccact caacgcctcc agctgcacca agaaagaata agttagctgt
    39181 ttcagtcacg ggttcatgac actcctttca ttaaaataat cgctagtgtt atatagaaaa
    39241 aaaacgacga ggttaggacc tactgaattt atgcgatagg catggtgcgt tcatatctca
    39301 actatttaat aaagttcaaa gatattacta tatatggcat catgaattat gtcctatgcg
    39361 ctttgtcccg acctctttta tctgttaatt atccagcaaa tgtcatgaat gcaattacta
    39421 caccgatgat aggtaatgcc tcaactaaac ctacaccaat gaacatgata cccattaatt
    39481 gaccacgtgc ttctggttga cgtgctacac cttcaactgt tcttgaaacg attaaaccgt
    39541 taccgatacc tgctcctaat gctgataaac caattgcgat tgctgctgcg attaaattca
    39601 taatttaatt tcctcctgtg attgtatata attattgaaa tttttagtgt tcatctgcca
    39661 ctttatgtga catataaacc atcgaaagca taataaagat atatgcttgg attgttccta
    39721 caaatattga aaatgcttgc caaacaatta atcctgggat actaataatc caaccccatg
    39781 ctggttcgtt aaagaataag ccagcaagta atgttaatag tatctcacct gcaaatatgt
    39841 taccgtacaa acgcagacca agcgttaatg ttgaagtgaa ctcttcaaaa acattaataa
    39901 ttgccaatgg ccaaaacggc tgtacataac ctttaagata ttgtttcgta ccacgcattt
    39961 taattccata aaagtgagtt aacagtatta tcgttgtaga caacgttaaa gtcactgttg
    40021 catcagctgt tggtgatttc caccacaatg tgtgatcttt cgttactatt gaaaacggaa
    40081 gacctaacat attagctata aaaatgtaca gaatcagcgt tactgctaag aagtggaatt
    40141 gaccaccttt tttccaagcc atgttacctt caatgattcc cctcacgaaa tcaaaaatcc
    40201 attcaacgaa attttgtttg ccagttggtc tttttttcaa attacgcgta caaatgatag
    40261 caagtagaaa aacaagaaac gccgtaacaa gtatcatcaa tatacttgat aaattgaaaa
    40321 cgatatcaaa accgaataaa ttccaactca cgagcgggga tttgtgatcc ataatctcac
    40381 ctctttctta atttatttgc tcacctttag taaaggacgt atgataacta caacatatga
    40441 aatcattaaa ccaataatta taccgattat acttacatgc gatttattaa agtaccaaat
    40501 catacaggca ataattgcaa ctaagtatcg ccacatattt ccagttgaaa tatgcatagt
    40561 gtctggtctt ttagctttgg ctaagtagct ctcaaatata acagtattga ttgcagaacc
    40621 agtaacacca atgattaatc ctaagataaa tgcatggtgt gtaaaaaggt ataatcctcc
    40681 caatattatt aataaataca aataatattg aatgtactgt ttaaaaatgt tgtagaaacg
    40741 actcatttta tatgcctccg tgtgactgat atcaatcatg aaaaccgttc cactcataca
    40801 aactaaatga taatataggc gatttgtggt gtcaattcta ttcaaattgt tgtaaaaaaa
    40861 taagcatgtt cagacacaat ttagacacat ttagacaaaa agtctttttt aattgaaatc
    40921 gcttggtttt tcagtgatta aattcaaata atacttaata tgattaacta ttctttctga
    40981 agcaaatcca tcaccgtaag gattagatgc ttcagacatt tgatgatata atctctcatc
    41041 atcgattaat tcttttgcag cttgatatac gtcttgttca tgcgtaccaa caactttcaa
    41101 cgtaccagca tcaacacctt caggtcgttc tgtaacgctt cgcaatacta ggacaggctt
    41161 attaaaagat ggcgcctctt cctgtatgcc tcccgaatcc gtcaaaataa aataggattg
    41221 tttagcaaaa ttatggaagt ctattacatc taagggttca atcaattcaa ttctgtcatg
    41281 cccccctaaa atcttatgag ccacatctcg gacttttgga tttttatgca ttggatatac
    41341 taacgctaaa tcggtgtact catctatcaa gcgtctaact gctttaaata tattttccat
    41401 aggttgacct aaattttcac gacggtgtgc tgtcataaga atgaactttt tgtcatggta
    41461 tttatccatg atgttagatt tataattgtc atcaactgta tatttcatag catcaatcgc
    41521 tgtattacca gtgacaacaa cactttctga atatttccct tcatttaaca aatgcgatgc
    41581 agcattttta gtaggtgcaa aatgtaagtc agctaataca ccaactaatt gtctattcac
    41641 ctcttctgga aaaggtgaat atttatcata acttctaagc cctgcttcaa cgtgtccaat
    41701 cggcacttgg ttataaaatg ccgctaaacc acctgcaaat gtcgtcatcg tatcaccatg
    41761 tacaagtacc atgtctggtt tttctaattg aatcacttgt tttaattgag tgattgattt
    41821 agaagttatc tcagaaagtg tctgtcctga tttcataata ttcaaatcgt attttggttt
    41881 gatttcaaag gtacttaata ctgaatcaag catttctcta tgctgtgctg taacaacaac
    41941 aattggctcg agcatttttt cttgttccaa agctttaact aaaggagcca tctttatggc
    42001 ttcgggtctt gttccaaata tggtcataat ctttttcatc aaactactta tctccgattc
    42061 ttctatttag taccaaacaa tctatctcca gcgtcaccta accctggtgt gatatatgct
    42121 ttgtcattta gcttttcatc aagtgcagca atataaatat ctacatctgg atgtgcttca
    42181 tgcatctttt ctacgccttc tggtgctgca attaaacaca tgaagcgaat atttttagcg
    42241 ccacgtttct tcaatgaagt aatagcttca attgctgatg cgcctgttgc taacatagga
    42301 tcaacaacaa tgatttgtct ttcagtaata tcttgaggta acttagcaaa atactctaca
    42361 gcctttaatg tttcgggatc tcgatacaaa ccgatatgtc caactctcgc tgcaggtact
    42421 aaacttaaaa taccatcagt catacctaaa ccagctctta aaattggaac gatagctaat
    42481 tttttaccag ctaatcgttt agccgtcatt ttagttacag gcgtttcaat atcaacatcc
    42541 tgaagctcta agtctctagt tacttcatat gccatcaaca taccaacttc gtctacaagt
    42601 tctctaaatt ctttagtacc tgtatttacg tctctaatat agcttagttt gtgttgaatt
    42661 aatggatgat cgaaaacgtg tactttactc ataaaaatta ctcctatctt tgtgtatgtt
    42721 tattgatata gaggatattc agctgttaat ttcgcaacgc gttctttagc ttgttgtaat
    42781 ttttcttcat ctttactatt tttcaatgct aaactgatga tttttgcaac ttcctcaaaa
    42841 gccttttcat caaatccacg cgttgttgca gcaggtgtac ctaaacgtat accactcgtt
    42901 acaaaaggtt tttcttgatc gaacggaatg gtatttttgt tacatgtgat accaactgaa
    42961 tctaaagtct cttcagcttc tttaccagta agtcctatag acccttttac atcaacagct
    43021 actaagtgat tatctgtacc accagaaaca attctaaatc cttcattaat taatgcttct
    43081 gcaagaactt ttgcgttttt aaccacttgt tgttgatacg ttttgaaatt attttctaac
    43141 gcttctccaa aagcaactgc ttttgctgca ataacatgct caagaggtcc accttgaata
    43201 ccagggaaaa ttgttttatc tatgtctttt ttatattctt ccttacataa aatcatacca
    43261 ccacgtggtc cgcgtaatgt tttgtgtgtt gtagttgtta caaaatcagc atattctact
    43321 ggatttggat gtaaacctgc cgctactaat cctgcaatat gtgccatgtc taccattaac
    43381 ttagcgttta cttcatctgc gatttcttta aactttttga aatcaattgt tcttgaatat
    43441 gctgatgctc ctgccacaat aagcttaggc ttatgctcta acgctaattt acgaacttca
    43501 tcataattga ttcgttctgt gtctttatct actccatatt caacgaaatt gtagaattta
    43561 ccactaaaat taacaggcgc tccatgtgtc aagtgaccac catgactcaa attcatacct
    43621 aaaactgtgt cgcccatttc taatgcaact aagtaaacag ccatgttcgc ttgtgaacct
    43681 gaatgtggtt gaacattgac atgttcagct ccaaacaacg ctttagcacg atcaattgcg
    43741 atgctttcag taacatctac aaactcacag ccaccataat atcggcgtcc tggatagcct
    43801 tcagcatact tattagtcaa cactgaacct tgtgcttcca taaccgcttc cgatacaaaa
    43861 ttttccgatg cgattaactc tatgttgcta ttttgtctct gaaattctct ctcgattgct
    43921 tctgcgataa ctttatcttg cttggtgata taagacataa aatctcccct tctttcaaaa
    43981 aaacttattg gtatttagca cgttcgccac caatcttttt cggcctagat gtggcaatag
    44041 ttacaattgc ctgtcctact tgctttactg aagtccttac aggtacacat acatgtttaa
    44101 tatgcatgcc tattaacgtt tgaccaatat caattccaca aggaacagta atatgttcga
    44161 ccacgattgg atccttcata tgctgaaaag cgtatgttgc caaactccct ccagcatgta
    44221 catctggaac gacggaaact tcttccattg ttaatggatt aaactgagat ttttctattg
    44281 ttatcgctct gttgatatgt tcacatcctt gaaaagcaaa agtaacgcct gtctctttac
    44341 tcacaacatc taatgcatta aaaatagttt ctgcaacttc catcgaaccg acagtcccta
    44401 ttttttcgcc aatgacttcc gatgttgaac atccaattaa acatatatct cctttattaa
    44461 aaaaggacat atcttttaat tcgtgtaata acattgtcaa atctttcata aaagcccacc
    44521 cttcctaaaa ataaaaaagg aatatagcaa agtgctacac tcctctatta taacttattt
    44581 aactgttaac atatactaat tatacagaat tcctactagc aaataatatc ttttaatttt
    44641 aaaattaaac ttacaagttc ttcataggta tgtacataca tttcttttgt accgccgtat
    44701 ggatctataa cttctcctgc ttcttttaca tattcatgca atgtgaaaac atgattttgc
    44761 aaaccaaagt gtgcctctat taattctttg tgcgaatacg acatagtcaa aataatatct
    44821 gctttcaaat ctgcttcagt aaattgttgc gataaggtcg tttcagctaa atgatgttct
    44881 tcaactaagt cttcaacata attcgaaaca ccttgattgt tcacagcgaa tatacctctt
    44941 gattcaaatt gatgatttgg cataacctct tttgcaatac tttccgctaa tgggctacga
    45001 catgtgttac ctgtacaaac gaataaaatc ttcatagttc acatccttta ataatgtgat
    45061 tacctgcagc ttttaacatg cgattcataa ttgcttctgt attatcattc agctcaaagc
    45121 cgtatatata cgccgctgaa atattttcat tttcatcaag tgaatgtaac acatcataaa
    45181 gattatgact tgcttgttta acatcattgt catcctgaca taattgaatg aattgcgctt
    45241 cacttggtat aaacgccacc ttattactcg gcacaataaa agctatagaa gaccaatctt
    45301 taccgtcatt tccaattttg ctctcgatat ctgtaataat tgtaagtggt gtattgggtg
    45361 agtaatgctt atacttcata cctggtgcaa ttggctgttc agtatcatta taatcagcat
    45421 gggcgatact attcggaagt atttctgtta tcattgctgc tgttatagaa ccaggtcttg
    45481 caattttata aggaaaagat gtgcaatcta aaaccgtact ttctaatcct tcttcacttt
    45541 gttcagcttg aacaatacca tcgatacgtc cattcaaatc ttgatataca tgattgaaag
    45601 ttgttggtga aggtctacca cttaaattag cacttggagc agctagaggt tcatttatga
    45661 tttgtaataa ttgtctacct acagaatggc ttggcattct aacagcaact gatgataaac
    45721 ctccagaaac ttttcgacat agatagcctg gctttaacgg caatataaac gaaatagggc
    45781 ccggccagaa tgcctgcatt aacttttcta cgcgtggatc caaagtatat gtaaaatctt
    45841 ttaattgacc tttactgtgt atatgaacaa taagcggatt gtcagatgga cggcctttag
    45901 cttcatatat tttagctaca gcttcttcat ctgtcgcatt tgctgcaagt ccataaactg
    45961 tttcagttgg taaacctatt aaaccaccgt ttaaaacaat gtcttttatt tcattaattt
    46021 taggatattg ctgtaaatct tcattatatt ctctaacatc ccaaatttta gtatccaact
    46081 taatcacgcc tttcttattt atcataatac aaagcaaaaa gctatgcact taactaatca
    46141 tagcaaaggc ataactttta attaccattt aaatgagacg attcgatcgt ggccatttat
    46201 atctttaata atgtcgattt ttttgtcagg aaatttattt aaaattattg atttaagtgc
    46261 ctcaccttga ttgtaaccaa tttcaaaaac aactgggctg cctttttcca taacgtgagg
    46321 taaatcttca atgattgatt cataaatagc atatccatgg ttatctgcaa acaatgcctg
    46381 atgtggttcg aatctcgtaa ccgttggaga catcgtaacc atatcttttt catctatata
    46441 aggtggatta gatatcaagc cgttcaactt gataccttct ttaattaagg gctttaatgc
    46501 atcccctgtt aaaaactgta tttgtgattg atgcttctca gcattattac gagccatatt
    46561 cattgcttca agtgaaatat cagtagcaat aacatttaaa tccggctttt cacatttcaa
    46621 agtaattgca agtacaccac tacccgttcc gatatctacg attgttgcat catcttctaa
    46681 ctgttgtaag aaatgcaaca ttacttcttc agtttcaggt cttggtatca aacaatttga
    46741 gtttacatca aacgttctac cataaaatga ggcaaagcca actatatact gtataggctc
    46801 tcctaataac atacgttgta aggctaagtc gaacttcata atcatcgctt tcggcatatc
    46861 atcatgcatg tggactacaa agtccgtacg cgtccattga aatacatcta acattaacca
    46921 ttcagctcgt gtttgttcaa accctttttg ttgtgttaaa tgaattgctt catctaactt
    46981 ttctttataa ttcaccatta ttaagttctt tcaatttatc tgtctgctct gataaagtca
    47041 gtgcatctat aatttcttct aaatggcctt ccataatttg ccctaatttt tgaagcgtta
    47101 gacctatacg gtggtctgtt acacggcttt gtggataatt ataagttcga atacgttctg
    47161 aacgatcacc agtaccgact gctgatttac gttgtgacgc atacttttgt tgttcttctt
    47221 gaactttcat atcgtataaa cgtgctttta acactttcat tgctttttca cggttttgaa
    47281 tttgagactt ctcagaagat gttgcaatga caccagttgg taaatgggta atacgtactg
    47341 cagagtcagt tgtgtttacg tgctgaccac ctgcaccact tgaacgatac gtatcgattt
    47401 ttaaatcttc atttctaatt tcaatttcta catcttcaac ttctggtaaa actgccactg
    47461 tagctgttga agtatgaata cgtccacctg attctgtttc aggcacacgt tgaacgcggt
    47521 gcgcaccatt ttcaaatttc aatttactat acgcgccatt accagaaact gagaaactaa
    47581 tttctttgta accaccatgg tcactttcag acgcttctac tatttcagtt ttgaatcctt
    47641 gtgattctgc atactttgaa tacatgcgca ttaaatcacc agcaaaaatc gcagcctcat
    47701 caccaccagc tgctgctctt atttctacaa taacgtcttt gtcatcatta ggatctttag
    47761 gaatcaataa tattttaagc tcttcttcaa gatttggaag ttcagcttta ataccactac
    47821 tttcctcttt taacatttct acttcttctt tatcatcagt ttcacttaac atttcttcaa
    47881 tatcagctaa ttcttctttt ttagctttat agttacgata aacatctaca gttttttgta
    47941 aatcagcttg ctctttagaa tatttacgta atttatctga atcatttaca acatctgggt
    48001 cacttaacag ttcatttaac tgttcgtatc tttcttctac aatatctaat tgatcaaaca
    48061 cttataattc ctccttatta ttatcactag gtgctacgat atggtgcgcg cgacaacgtg
    48121 gctcataact ttcattggca cctactaaga taatcggatc atcgatttta gctggtttac
    48181 catttattaa tcgttgcgtt ctactagatg aagaaccaca aacagcacaa actgcttgaa
    48241 gtttcgttac ttgttcactg acagccatca atttaggcat tggttcgaac ggttcgcccc
    48301 taaaatccat atctaatcca gcaacaataa cacggtgtcc atctgctgat agtttttcta
    48361 ctatacttac aatttcatcg tcaaaaaatt gtacttcgtc tattcctata acatcaacat
    48421 tagttaagtc gtgcgtcata atttcacttg ctttagaaat attaatcgct tcaatggcat
    48481 taccattatg agagaccact ttttctttat gatatcgatc atcaatcgcc ggtttaaata
    48541 caacgacttt ttgtttagca tatatgcccc ttcttaaacg tcttataagt tcttcggatt
    48601 taccgctaaa catactacct gtaatacatt ctatccaacc ggaatggtaa gtttcataca
    48661 ttgagagttc cacctttttc aaaacataat cgctttatta tatcatattt caaatattca
    48721 taaatgtctt tttcataatt atatcgatat tgtacatgaa caattatttt ataaatttgt
    48781 cttgcatatt tatatacagc cattctattt tgttgcttct tattcgtgcg atgtttctac
    48841 tcaatacaaa ctttaataaa ggggaaaatt tatcattatg tattaacaac atgttattga
    48901 gtcacttctt tgaacacagt cgtaagtaaa tacccttact acacttataa ataaaaaagc
    48961 tgtctacaat tccattatag gaagcagaca gcttaacgat tactatatta gttgtttgat
    49021 ttaagaccaa actttttgtt gaaacgttcc acacgtccat ccgcagcagc gaatttttga
    49081 cgtccagtgt agaatggatg tgaatcagat gaaatatcta aacgaataac tgggtattct
    49141 tttccatctt cccactccat catttctgaa gatgtttttg tagaaccgct taagaatttg
    49201 aagtttgtag tagtatctaa aaagattact tggtgatact caggatgaat tccttgtttc
    49261 attattttca gctcctttgc cctgaaccat ctggaacaga gttatttgtg agtttttacc
    49321 caatactgtg taattataaa ggtaattaat tcaaaacgca agcccctata taatgtttat
    49381 taaattatag gtcgacccgt tttagtactt tcttctgcag acttttgtag ctgcttgaag
    49441 aaatcttcat tattcttaga ccttttaagt ttgcgaataa atctttcagt aaagtctgtt
    49501 gagtcagtga atagatttct taattgccat aatgtgtcta attcagattt acttatcaac
    49561 aattcttctt tacgcgttga acttctgcca atatcaattg cagggaagat acgacgttca
    49621 gacaatttac gatctaaatg taactccatg ttacctgttc ctttaaattc ttcgtaaatc
    49681 atatcgtcca tacgtgaacc cgtatcaact aatgcagttg caagtattgt taaacttcca
    49741 cccgcttcaa tatttctcgc tgcaccgaag aatgcttttg gtttgtgtaa agatgcagga
    49801 tctaaaccac ctgataatgt acgaccactt ggtggaataa ctaagttata agcgcgtgct
    49861 aatctcgtta tagaatccat taaaataatg acatcttccc caatttctac taaacgcttt
    49921 gcacgctcaa gtaataattc agctacttta acatggtgtt ctggtggttc gtcaaacgtt
    49981 gaatgaacga cttcagcagc ttctactgag cgttctaaat ccgttacctc ttcaggacgc
    50041 tcgccaacta acaaaataaa tagctttgca tctggtttgt tcgtactgat tgcattcgct
    50101 atttctttta ataacgatgt tttacctgct ttaggtggcg ccactattaa accacgttga
    50161 cctaaaccaa tcggtgttac taaatccatg atgcgcgttg aataattttg tgtttctgtc
    50221 tctaatttaa tacgctcatc tggataaagt ggtgtcaaag cttggaaatg cggtcgtttc
    50281 ttcacttctt ctgcgttatg gtcattgaca aagtcaactt gtaataagcc ataatatttt
    50341 tcgttatctt taggttttct aactttccca gttactttat ccccacgttt aatttcaaaa
    50401 cgacgaattt ggctagcaga tatataaata tctttttccc ctttagaata gttcactgtt
    50461 cttaaaaaac cataaccatc cggttgtata tcatctaaga taccttccat ataatagtta
    50521 ccatcttttt ccatttgtgc ttccataata gctagaacaa gttctttttt atttaattta
    50581 ctatagttcg tcaacttaag agttttagct ttttgagtga gttccttggt agtatagttc
    50641 ttgtacaatt cgtggaatga ttcatactga ggagatgtac gttctctttc aggcatatag
    50701 tttacaccca tttcattatt ttttagttag taagtaatag ataggcatga caatgcataa
    50761 ataactgtgc tagcacaatc ccattatgct aatccccaac cttttaaata catgaataac
    50821 tatagcaaat ttttatttta attacaaaat ctatttataa aaattgcaaa attaaaacag
    50881 tacacaaaac ttaattagtc atgtgcactg ttttattgcg atttttaatt caaaaatcaa
    50941 atttatttaa aatagccagc tatagatttc acttctaaga actcttcaat accataatcg
    51001 ccccattcac gacctaaacc agattgttta tagccaccaa atggtaagtc tggctttcta
    51061 cctgcttcgt ttatttctac tgtacctgct tcaatagaac gagctacttt atgcaatgtt
    51121 tctttgtcct taccaataac atatcctgct aaaccatatt ttgtatcatt tgcaatttga
    51181 atcgcttcat ctaaatcgtt ataagtgata actgacatta ctggcccaaa aatttcttct
    51241 tgtgctatcg tcatttgatt atctacatta ataaaaattg tcggacgtgc aaagtatcct
    51301 ttttcaagtc cttctggttt accaggacca ccataaaata attcagcacc ttcttcaata
    51361 cctttattaa tataattttg tacttgatca aattgttttt tactaatgat agggcctact
    51421 tgtgtaccat cttctcttgg attaccgaca cgcacttggc taaattgttc ttttaattca
    51481 gctaagaatg catctttaat tttgttaggc actaaaacac gtgtaccagc tgtacatact
    51541 tgaccagtat tattaacaac tttgcctgtt gttgctttag ccgcttcttt aatatctacg
    51601 tcatctagga cgatgtatgg tgatttgcca ccaagctcta atgatacctt tttaaaatct
    51661 ttagcagctt tttccataat tttagaacca gtagggcctg atcctgtaaa tgacatcatg
    51721 cgtactttag gatgttcaga taaaggattc ccaacgcctg caccatcacc attaacaagg
    51781 ttaaatacac ctttaggaac accgacttta tcaaaaatct cagctaaaat aacagctgca
    51841 aatggtgttt cttcagatgg tttaagtaca actggactac cagccgcaaa tgctgctgct
    51901 aattttaatg atgtttggtt tgtagggaag ttccacggtg taattaagcc agatacaccg
    51961 attgcttctt taacaactaa atcatctccg cggcgttctt caaattcgta attatctaat
    52021 gcgtctctcg ctgcaacaaa atggtttagt cccatttgat aatggacacg ctcagataat
    52081 gataaaggag cacctaactc atccgtaata gcttgtacaa tatcgtcttt tctgttttca
    52141 tattctttta caattttatc taataacgct tgtctttctt tcacagatgt atgacggaac
    52201 tctaaataaa cattgtctgc cgcctcgacg gctttatcaa catcagcttt attaccttta
    52261 gcaactttcc cgattacttc ttcggttgct ggatttataa cttctatcgt ttcattacta
    52321 ttactttcta cccattcgcc attaatgtat tgctttgtgt agtctctcat tattctttca
    52381 ctcctcaaga atgatttata tattatttat ccgtaatgca
//
LOCUS       DATTQF010000004        50085 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.004, whole genome shotgun sequence.
ACCESSION   DATTQF010000004 DATTQF010000000
VERSION     DATTQF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 50085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.004"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..174)
                     /locus_tag="VB241_000173"
     CDS             complement(<1..174)
                     /locus_tag="VB241_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243508.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKS"
     gene            complement(374..3163)
                     /locus_tag="VB241_000174"
     CDS             complement(374..3163)
                     /locus_tag="VB241_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP1243509.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFI
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            3376..3657
                     /locus_tag="VB241_000175"
     CDS             3376..3657
                     /locus_tag="VB241_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243510.1"
                     /translation="MVLITYQIILFLIISLSYYLTLNHFMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIITFSTFVLVHLALKLFFNI"
     gene            complement(3852..4727)
                     /locus_tag="VB241_000176"
     CDS             complement(3852..4727)
                     /locus_tag="VB241_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1243511.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4730..6184)
                     /locus_tag="VB241_000177"
     CDS             complement(4730..6184)
                     /locus_tag="VB241_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1243512.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHNHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(6196..7092)
                     /gene="murQ"
                     /locus_tag="VB241_000178"
     CDS             complement(6196..7092)
                     /gene="murQ"
                     /locus_tag="VB241_000178"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEP1243513.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(7092..8147)
                     /locus_tag="VB241_000179"
     CDS             complement(7092..8147)
                     /locus_tag="VB241_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP1243514.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     IRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDSVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            8732..10777
                     /locus_tag="VB241_000180"
     CDS             8732..10777
                     /locus_tag="VB241_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HEP1243515.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGEALQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNEFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQTGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(10756..10857)
                     /locus_tag="VB241_000181"
     CDS             complement(10756..10857)
                     /locus_tag="VB241_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243516.1"
                     /translation="MMLQNTIAMKKQARHLMKVYAPVYSKHYLASNN"
     gene            11049..12689
                     /locus_tag="VB241_000182"
     CDS             11049..12689
                     /locus_tag="VB241_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HEP1243517.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENAATEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLVSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLSSLLQQLTDISYTNN
                     ASFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     SNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQEVF
                     NTINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            12756..13313
                     /locus_tag="VB241_000183"
     CDS             12756..13313
                     /locus_tag="VB241_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEP1243518.1"
                     /translation="MINFDKTALVLIDLQQGILKMDYAPHTAENVVQNANKLIEAFRK
                     NNGFIAFVRVNFYDGKDALQPNAMISLPPKEGDDYSHFHYLLDKRDDDFVIDKRQFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHVGVDTTARDAYQLNYDQYFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDLLNILN"
     gene            13591..14946
                     /gene="brnQ"
                     /locus_tag="VB241_000184"
     CDS             13591..14946
                     /gene="brnQ"
                     /locus_tag="VB241_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP1243519.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            15199..16383
                     /gene="rocD"
                     /locus_tag="VB241_000185"
     CDS             15199..16383
                     /gene="rocD"
                     /locus_tag="VB241_000185"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEP1243520.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVITEGKGGKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSDVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKSGFHP
                     LLQGTKTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVEQLIKRGILCKDTHRTIIRLSPPLVIDKEEINQIVAAFQDVF
                     KN"
     gene            16422..17453
                     /locus_tag="VB241_000186"
     CDS             16422..17453
                     /locus_tag="VB241_000186"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEP1243521.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSLDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMARGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLKQSPVYP"
     gene            17465..18706
                     /gene="argJ"
                     /locus_tag="VB241_000187"
     CDS             17465..18706
                     /gene="argJ"
                     /locus_tag="VB241_000187"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEP1243522.1"
                     /translation="MKHQETTSQQHNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DAQQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHINDANFATPGAFNEA
                     ILTTDTCTKHIAVSLNIDGKTITIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANQQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            18722..19492
                     /gene="argB"
                     /locus_tag="VB241_000188"
     CDS             18722..19492
                     /gene="argB"
                     /locus_tag="VB241_000188"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEP1243523.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQQQCSAIGLCGLD
                     AQLFEIKRFDQQYGYVGVPTTLNIDSLSYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIAASLEAPIYVLSNIAGVLINDVVIPQLPLADINQYIEHGDIYGGMIPKVLDAKN
                     AIKNGCPKVIIASGNKPNIIEAIYNNDFVGTTILKSSV"
     gene            19777..20256
                     /locus_tag="VB241_000189"
     CDS             19777..20256
                     /locus_tag="VB241_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HEP1243524.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMQLPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(20585..21229)
                     /locus_tag="VB241_000190"
     CDS             complement(20585..21229)
                     /locus_tag="VB241_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEP1243525.1"
                     /translation="MKVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(21242..28417)
                     /locus_tag="VB241_000191"
     CDS             complement(21242..28417)
                     /locus_tag="VB241_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEP1243526.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNIEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHLNRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKSVLQNMSQLQCAKSSLSLETIFHCYHQMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITKENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNYVAVQFDGVFITYQTLNARANDL
                     AQRLRNQYGVEPNDRVAVIAEKSIEMIVAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQVVYENGKRDINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEKTTILLSGTIAFDAATFEIYGALLNGGKLIVAKT
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQRPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNIPFLWRLSSE
                     LNVAQLRQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFKEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSLDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDCDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQIRDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFDEVVSRQGNHVALVMNDLTMTYETLHNYVDAIAHMLLSNGVGKGQPVALIT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPKKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGDQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITYIDCIPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGKTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVKNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSLAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKCKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTKLTLKIMD
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(28864..30114)
                     /locus_tag="VB241_000192"
     CDS             complement(28864..30114)
                     /locus_tag="VB241_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1243527.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKKHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKIVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTTLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(30500..31525)
                     /locus_tag="VB241_000193"
     CDS             complement(30500..31525)
                     /locus_tag="VB241_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEP1243528.1"
                     /translation="MKIVALFPEAVEGQDNQLLNTKKALGLKTFLEERGQEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(31699..32058)
                     /locus_tag="VB241_000194"
     CDS             complement(31699..32058)
                     /locus_tag="VB241_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEP1243529.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESVVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(32272..33303)
                     /locus_tag="VB241_000195"
     CDS             complement(32272..33303)
                     /locus_tag="VB241_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEP1243530.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEATLR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVAVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEGFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(33316..34077)
                     /locus_tag="VB241_000196"
     CDS             complement(33316..34077)
                     /locus_tag="VB241_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243531.1"
                     /translation="MTRLTINKIILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(34074..35048)
                     /locus_tag="VB241_000197"
     CDS             complement(34074..35048)
                     /locus_tag="VB241_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1243532.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRASKEPSNNAQHQQVIKIGYLPI
                     THSANLMMTKKLLSQHNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEALREQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKAVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(35062..35802)
                     /locus_tag="VB241_000198"
     CDS             complement(35062..35802)
                     /locus_tag="VB241_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243533.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTESTIILVAHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPGYYL"
     gene            complement(36144..36656)
                     /locus_tag="VB241_000199"
     CDS             complement(36144..36656)
                     /locus_tag="VB241_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEP1243534.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKASSLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEAGISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            36836..37039
                     /locus_tag="VB241_000200"
     CDS             36836..37039
                     /locus_tag="VB241_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243535.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDTIKDIDENHLDDAT"
     gene            complement(37101..38060)
                     /locus_tag="VB241_000201"
     CDS             complement(37101..38060)
                     /locus_tag="VB241_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP1243536.1"
                     /translation="MNGKKANTINRYKYFHHVDHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDSIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIIADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(38707..40194)
                     /locus_tag="VB241_000202"
     CDS             complement(38707..40194)
                     /locus_tag="VB241_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEP1243537.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPNGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            40621..41004
                     /locus_tag="VB241_000203"
     CDS             40621..41004
                     /locus_tag="VB241_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEP1243538.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNNKIEE"
     gene            41011..41337
                     /gene="isdI"
                     /locus_tag="VB241_000204"
     CDS             41011..41337
                     /gene="isdI"
                     /locus_tag="VB241_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HEP1243539.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TKDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(41402..42547)
                     /gene="cap8P"
                     /locus_tag="VB241_000205"
     CDS             complement(41402..42547)
                     /gene="cap8P"
                     /locus_tag="VB241_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HEP1243540.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGITANALAKLDSIINGEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKESIFITGNTVIDALSTTVQNEFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDALEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRRK"
     gene            complement(42624..43886)
                     /gene="cap8O"
                     /locus_tag="VB241_000206"
     CDS             complement(42624..43886)
                     /gene="cap8O"
                     /locus_tag="VB241_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HEP1243541.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPSYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(43940..44827)
                     /gene="capN"
                     /locus_tag="VB241_000207"
     CDS             complement(43940..44827)
                     /gene="capN"
                     /locus_tag="VB241_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HEP1243542.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KGYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTLLINMPSMLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(44827..45384)
                     /gene="cap8M"
                     /locus_tag="VB241_000208"
     CDS             complement(44827..45384)
                     /gene="cap8M"
                     /locus_tag="VB241_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HEP1243543.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTAYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(45395..46600)
                     /gene="cap8L"
                     /locus_tag="VB241_000209"
     CDS             complement(45395..46600)
                     /gene="cap8L"
                     /locus_tag="VB241_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HEP1243544.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NKHVYKNAENVIVLGTEMKNYLLNHQISKNPDNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(46634..47872)
                     /gene="cap8K"
                     /locus_tag="VB241_000210"
     CDS             complement(46634..47872)
                     /gene="cap8K"
                     /locus_tag="VB241_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HEP1243545.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISAWL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEY
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTVVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            complement(47881..48438)
                     /gene="cap8J"
                     /locus_tag="VB241_000211"
     CDS             complement(47881..48438)
                     /gene="cap8J"
                     /locus_tag="VB241_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HEP1243546.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKVIKKI"
     gene            complement(48435..49829)
                     /gene="cap8I"
                     /locus_tag="VB241_000212"
     CDS             complement(48435..49829)
                     /gene="cap8I"
                     /locus_tag="VB241_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HEP1243547.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQDYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVILPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSLLFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGHSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFVDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            complement(49822..>50085)
                     /locus_tag="VB241_000213"
     CDS             complement(49822..>50085)
                     /locus_tag="VB241_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEP1243548.1"
                     /translation="SIRMYYALFLNKPIITTDDTFTATEANKFGLGFSVNPENLKGIG
                     DELMDWYNNLNVMDINHKREAYRNDVIENNKQFYQEIGRIFNE"
BASE COUNT        16189 a         9461 c         7504 g        16931 t
ORIGIN      
        1 agacttttta agatagttat ataaagactt cattttctgt attacattga tatttttagg
       61 cccttgtata taaaaatcat atgcctcatc aacaacatca cttttatttt tgattgcaat
      121 ttgatgtaat atcttttgac taaaaacacg attaatcata tttctattat ccatagctac
      181 gcacctcctt caacatacat ttaatactta tcctgttaat attataaact attttaaatg
      241 cctatagaca ttatttctat acaaatacga tgataaaatt gtattgaaac caaatgatga
      301 ttgccaaaca catgagcctt ttctaatgaa ataataatgc cccctacact aatctagcga
      361 gggggctgaa tcattacaca ccgtattttt cagttgtttc tgccacgaaa gattcaatgg
      421 cttttcttgc tttacgtttt tctttcaatg gcaaatcacc aatcattttt ttaaggtgat
      481 gtgggtttac aacaccacta tactggtagt catttgaaat tgtttttagg gcttgttcat
      541 cgatagatct ctctccagca aatcctttga actccgcttc ttttttaata ctttcgaaat
      601 taacatattc ttgatcgata tcatcatcct tatttaaaga aggtacaaca ttgtcgatga
      661 attctctaat tagatctcgt tttaacctca atgtcggatc atctgcatga tctaaaatac
      721 gtctaatttg ttcttggtta cgacgttgtt ccgctttgtc ttcaagatca atttgtctca
      781 atatattcat aatataattc acattaatcg tatcattacg catcatttct atttcgaaat
      841 caatatcatt taaaatggat actttatttt tctcagccgt cgctcttttt acttgatcgt
      901 acacggctaa atatttactt ttatagtctt cattctcttg ttcatccatt ccaatttcat
      961 caattgtaaa ctcaaactcg tcaaatgctt ttaaacgtaa tattatttta gctaataaac
     1021 gataagcttc aacaaagcgc tttagctctt cttcatcttg aatgtcatca accatgtgtg
     1081 gtgtcggcac aatcatttta agctcacgat aagcgtcaat aaattctttt ttatactctt
     1141 cataactacg cattaaaatt gtatccgtat catttgtttg tgagaatact ctcagtgcat
     1201 cgtctgtctc ttttttcaag tcacgatagt ttacaatttt accaaatggc tttgattctt
     1261 tttcaaccct atttgtacgt gaatacgctt gaattaaatc atgatacatt aaattcttat
     1321 caacatataa agtgttcagt actttactat caaaaccagt taagaacata ttaacaacga
     1381 ttaagatatc aattttacta tctttaacgc cctttttaac gttttttgaa atatggttaa
     1441 aatactcatt agttgtgtct gttgaaaaat tcgtctcgaa ctttttatta taatcactaa
     1501 tcattatctc taatttttca cgtgaatgat atggcacttc accatcacga tcatcttcat
     1561 taggtttaaa cgtaaatata ccagctaccg ttaatggttg ttccaacttt ttgttaagtc
     1621 gcttaaatgt ctcataatat ttaataagcg cgtgaatact ttgaactgta aatatacttg
     1681 aatattgacg attacgtgta tatttatcat gattattgat gatatgtcgt gttactaatt
     1741 ccacacgttt atccgctaac catacttctt ccgtatcaat tgcttcaacc atgctgttat
     1801 cttctgcttt taaagcttta tttttaaaag tattaatata gtcaactgag aaaccaagta
     1861 cattaccatc atgaatggca tctctaatta aatacgtatg taaacatcta ccaaaaatat
     1921 ctgcagttgt tctaccatct tgactactat tttctgggaa acgtggcgta ccagtgaacc
     1981 caaaatattg ggcatttttg aaatgttgtt taactagacg atgcatgtca ccaaattgac
     2041 tgcgatgaca ctcatcaata ataaatacaa ctttatttgt tttatactgt tctaataaat
     2101 gggcattacc ttgaatcgct ttagccattt tttgaatcgt cgttacaata agtggcaaac
     2161 ttttatcatt tagttggcgt accagttgcg aggtattaaa tgttttgtcg acagcaccct
     2221 tagcaaattt attaaattcc tcttctgttt gactatccaa gtctttacgg tcaaccaaaa
     2281 agataacttt cttaatgtca tcttgttgtg ataaaatctg actcgcttta aaagaagtca
     2341 acgtcttacc acttcccgtt gtatgccaca catatccatt attccctgtc tcagtcgctt
     2401 gttgaataag tgcttctacc gcatacactt gatacggacg cattgccatc agtattctat
     2461 ctgtttcatt aataatcata tagcgtgata tcatcttagc taattgacaa ggtctcataa
     2521 atgactcagc aaacgattgc aatgtattga ttcggttatt ctgtttatca ctccaataaa
     2581 acatgtgact cttcaatagt tcgctatcat tattagaaaa gtatcgcgtt tcaacaccat
     2641 tactaatgat aaacatttgt atgtagcgga ataagcctgt gtaattttgt ttacggtaac
     2701 gttttacttg gttaaacgcc tcattaatat caatgcctcg acgtttcaat tcaacttgga
     2761 caaggggtag tccgttgatt aatatcgtta catcataacg tgctttatat gtatcctcga
     2821 ccgatacttg attcgtcact tgaaacttat ttttacacca acttttcgta tctaaaaacg
     2881 acaaataaat ctcagactca tcatcacgtc taagtggtaa tttatcacgt aaaatacggg
     2941 cactttcgaa aatacttttc ccatcaatca tcgttaacag acgttgaaat tctttatctg
     3001 ttaagggatt gccttctaat ttgtccgcat gacgctcatt taaaatcgtt ctaaaattat
     3061 caagcaattg cttattatca cgtatcgtta ctctttcgta acccaattgt tcaagttgat
     3121 tcatcatttc attttctaat gcgtattcac tttggtatgc cattcatatc cccttccata
     3181 cactttctat tgctctaaat atatcataaa ctttaatgaa aaatgtttgt tttttatctt
     3241 caaacgtaaa tttattctaa ttttattgtc ttatatttta atatttgtgt ttgaggtaag
     3301 tcgtatacta aaatttgaat acaaataatc aaatcattga taaatttttt gcctacgatt
     3361 aatggaggga cttgaatggt gttaattacc tatcaaatca ttttattttt aattatcagt
     3421 ctaagttatt atttaacttt aaatcatttc atggcagtca ctgtaggtaa cttcacttca
     3481 atattcggca tgttcgcagc catactcttt atgtactact acctactcta taaaagtccc
     3541 gaatacaatc aacgcaaacg atttaaacat ttcattcata tcactaattt gataataatt
     3601 acttttagca ccttcgtatt agttcattta gcattaaaat tattcttcaa catttaattt
     3661 ctatactatg aaaaaagcaa agctcaaacc tgaactttgc tttaatttgt cacactttta
     3721 tcattttcaa aataacttct atgccagttt tacaaacttg tatcaacaat ttttcatcaa
     3781 gcaactaaat cacatcaaaa acttcaattg tttacgttgc aactaacttt atattagaac
     3841 gcacacaata tctaatgttt aaaatctatc gtcgcaagat gcttcctgta attatcaagt
     3901 gccattttcg attgggttat acaatctaga atcgcatgat aatttaatgc tacaaatcga
     3961 tagtacaata tatctaccgt gaataactgt gcaaatagtg acgttgtagc cgccatacgc
     4021 atttcatttt catcagttct gccataaatc aatgcatagt ctgcaatttg agccactgga
     4081 ttattagctg tactagatat agttatgatg ggaatactgt aatgtgtggc cacctgtgca
     4141 attgactgca attcactatg actaccttga ttcgtcacaa aaatcatgca atctctatca
     4201 tcatgcgtcg caaatgttga cacaagtaaa tgcgtttcat gtaataacct gacatttaag
     4261 ccaatacgag ataacttttg aaaaagatca ccaatagtca aactcgatgc gccaaatcca
     4321 aataaaaata ttgtcctggc atttttcaac acatcacaaa ttgcatcaat ttgcgcatcc
     4381 ataatattag tagctacaaa tcgcatcgta ttcgttgctc tagcaatcat tttatttttc
     4441 aaagtttcta cagattcatt ttcaatcaat tctaaatgtg gattggttgc aatatcttcg
     4501 ggtaagtatc gagatatcgc aatctttagc tcttgaaaac cttgatgtgt cattttccga
     4561 ctaaatctaa caattgatgc tgtactaaca ttcgtaacat ctgctaaatc attcacagtc
     4621 atatcaatga ttttatgtgg attctttaaa atgtaatcag cgattatctt ttctgtcttc
     4681 gtaaaatcac tcaactgctt atcaatgcga tataaaatat ttgtcatcat taatcaccca
     4741 acaaatctgt ctgtcgcatc gcctttgtcg ttccaaataa atatgtacaa acgaatccac
     4801 cagcatacgc agcaagtaat cctgcaatat aacctaaata catattatct gagattaatg
     4861 gtaatagtga cacaccactt gggcctattg ctttggcacc aatatgtcca attccaccta
     4921 ttacagcgcc accaatacca ccgccaatac aagcagttaa gaaaggtcga cctaatggca
     4981 aagtcacacc atagattaat ggttctccaa tacctaggaa accaactggc aatgcacctt
     5041 ttaaagtatt acgtaatgtt gtgttgcgtt tacatcttac ccaaagtgct aatgcggcac
     5101 ctacttgtcc agcaccagcc atcgctgcaa ttggcaataa gtaagtagca ccagattggt
     5161 taatcatttc tatatgaatt ggcgtaaaaa tatgatgaag ccctaacata actaacggta
     5221 ggaagcttgc accaatgata aatccactaa atacgccacc aatactaata attccgttaa
     5281 ctactgaaac taaactgtct gaaacaaaac ctgctaatgg cataaagata aagatagtta
     5341 atagtcctac aatcaacaat gcaatagtcg gcgttacaat aatatcaatc gcatttggca
     5401 caattttatg caatctcttt tcgacaatac ttaaaatcca aacggcaaaa ataacaccaa
     5461 taattccacc ttgtccaggt tgcaatggtt ctccagtgaa aacattcatt aaaatatttt
     5521 taccagcaat acccgttaat aacgttgtac caccaatcac gccaccaagt cctggtgtcg
     5581 caccaaattc tttagccgca ttaataccag tgaaaatagc taagtatgct aacataccgt
     5641 ctttaatgac attaaatact gttataagtt gcgtaatcca agcacctgaa atatagcctg
     5701 ccaccattaa gttactcagt actgctgcaa taccaccaat taatccagct ccaataaatg
     5761 caggaatcaa cggtataaag atattggcaa ttgatttcaa tactttattc aacttaccat
     5821 tcttttgttt tgctttatgt gcttctttat ttgcctttgc tttatcagct gcatatgatt
     5881 tatagtccat tttttcacta tcattgtgat tgtgtggtat tgggtcacct agtttaacac
     5941 cacttaactc agccatatga ttagccactt tattgactgt accaggtcca actacaactt
     6001 gaatgcgttc atcgtgtata acacccatga caccatcaat gtgccttagt tcttggtcat
     6061 ctactttatt ttcatctaat actttaatac gcacacgtgt catacagttc atgacactat
     6121 ctatattatc cataccacct actgcagcaa taattcgttc tgcaagttgt tgttctttgg
     6181 tcatttaaat ccctcctaag gttgtctatc tctgattgct cgtttaacaa tgtcaccatt
     6241 gtttaataac cgtcttgttg cttcttcctt agaaatgcca cacataccca taactgtcgc
     6301 aactttcaca tcatgctcag atacctgata taacgccatt gcttcatcat atgtgatagc
     6361 acatatttct tgaataatac gcactgaacg gtcgatcagt ttttgattgg ttgctttaac
     6421 atcaatcatg aggttatcgt aaacttttcc gacaccaacc attgtgatgg ttgaaatcat
     6481 atttaaaatt aatttttgtg ctgtaccaga ctttaaacgc gttgaaccag ttaatacttc
     6541 tggaccaact ttaacttcta ctggatactg cgcaatttca cttataactg catgttcatt
     6601 gcatgaaata gatactgttg tagcaccgat tgtattagca aatgttaaac cgcctataac
     6661 atatggcgtt ttgccactcg cggcaattcc tataacgaca tcttttgatg ttaaatctat
     6721 atttttcaaa tcttcttccg ctaatttttt gtgatcttcc gcaccttcta cagccatcgt
     6781 catagcatgt tgtccaccag caataatacc tataatttca tgaggatcag tattgaatgt
     6841 aggtacacac tccgctgcat ctaagacacc caatcgtcca cttgtacctg caccgatata
     6901 gatcaatcgt ccaccctttt tatactgtgc aattgttttt ttaattactt ttgtcaattg
     6961 tggtattgcc tttcgaactg ctaacgggac ttgctgatct tctttattca tcgtaattaa
     7021 tgcctcttcc acagtcattt catcaagatg catcgtcgct tcattacgcg cttcggtcgt
     7081 actattttcc atcacttctt acactcccta gttttttgaa aatcaaatgt atcattcggc
     7141 tcgatacaac ttaacagtgg taagtcttct ttaataattt gtgcaacaac attcacactg
     7201 tcatgtgcac taagcgtttg tctcacaatt tgcatttcac cttgataacg tccgttattc
     7261 aaattatcaa cggttactga accaatgcgt cgttgcgtcg taaactgtgg ttgaatcgaa
     7321 tgcggacata tttgtcttga cgtttccgaa cgaatgacat tctccggatt atccggacgt
     7381 actttatgac acatatcgaa aaggtaagtc actgtcgtat caaacacttc ttcaatacat
     7441 aacgtgaaat gcctatgctt acaaaaatct ataagttgtt ttgcctgcct tatttcaatc
     7501 aatgagtctc caactaacac ttcagataca ccagtttctt gtaataattt agctgcaacg
     7561 acaggatgac tatgtctcgt tgcttcaatt gttggcaagc ctttatgcaa aggacctcgc
     7621 aaaccactcc ctacaataaa accatatatt tgtgcctttg gattaaattg ataaatgagt
     7681 tcatttttct tattgaccaa gtcaacagat aatcccgtat ctggtcttgg ataatagtta
     7741 tgacaaaatg aaagtaatgt aaaatcattc aattgttgat gtaagcttgt taacaattcc
     7801 cgggaaataa tacttgcatt caaacagcac tttaaaccct gtgccattat cgcttcgatt
     7861 gcctcaattg atgtactatg atcgatacga atcataaatt gtgcatcata ttgtcgaaga
     7921 tgctcataaa aagatggtgt taatatagat ggattagcat ctatgaggta agtcacttgt
     7981 tcatgtttta ataaattgag tagttttgtg aaataatgat attttgtctc gtcatcttct
     8041 tctggtattt gtacagatgt aaaaatcatt tggtaacctt gtttaatcat tcgcttaata
     8101 tacgcttcat ctaaaggttg tcctaaatac actgaaaagc ctgtcaaagt agccctcctt
     8161 aacaatataa ttattaggaa aatatagttg agttgtgtaa tcgcttacat tttactataa
     8221 gagaaaacac attacaatat taatcagata aagcctgttc attgtaataa tcttacatat
     8281 ttctgtcaca agttaatttt tttaaacgac atatttttct tatcatttaa cgctcgaaaa
     8341 cagttgctat tgctgaattg ttacaacaac ttttatacat tttattcagg attatcttat
     8401 attatgtttt aataataatc tgtgaacaat taagagattt gaaattgaat ttaataattg
     8461 tattgaaaac gcatacttca ccatgctaaa ataggagtcg caaacaaata agattcaata
     8521 agatgtgatg gtaagctaga taaactaacg tatccgtttt atatactact tagtcaattg
     8581 cttcttttac attaattaca tacaccaacg tgttactaag taagattagg catgagttaa
     8641 attaagctgt gatggttacc aacacagtct atttgctcgt gtctttttta ttgaatctta
     8701 aataataaat acaactttgg aggttggaca agtgaggaag aaacttttcg gtcaattgca
     8761 acgtattggt aaggcactaa tgttacctgt tgcgatttta ccagcagcgg gtctgttatt
     8821 agctatcggt acagctatgc aaggcgaagc attacaacac tacttgcctt ttatacaaaa
     8881 tggtggcgta caaactgtcg ctaaattaat gacaggtgct ggtggtatca tttttgataa
     8941 cttgcctatg attttcgcat taggtgtcgc aatcggatta gctggcggtg atggtgtagc
     9001 agctatcgca gcattcgtcg gttacataat catgaacaaa acaatgggtg actttttaca
     9061 agtaacacct aagaacattg gtgatccagc gagtggttac gctagcattt taggtatccc
     9121 aacattacaa acaggtgtgt tcggcggtat tataatcggg gccctggcag cttggtgtta
     9181 taacgagttc tataacatta acttaccatc ttatttaggt ttcttcgctg gtaagcgttt
     9241 cgtacctatt atgatggcta caacatcatt tattttagca ttcccaatgg cattaatctg
     9301 gccaacaatc caaacaggat taaatgcatt cagtacagga ttattagact caaatactgg
     9361 tgttgccgta ttcttatttg gtttcatcaa gcgtttatta attccattcg gtctacatca
     9421 cattttccac gcaccgttct ggttcgagtt tggttcatgg aaaaatgcag caggtgaaat
     9481 tattcacggt gaccaacgta tctttatcga acaaattcgt gaaggtgcac atttaacagc
     9541 tggtaaattc atgcaaggtg aattccctgt tatgatgttc ggtttacctg cagcagcttt
     9601 agcaatttat cacacagcta aacctgaaaa caagaaagta gtagccggtt taatgggttc
     9661 tgctgcttta acatcattct taactggtat tacagaacct ttagaattct cattcttatt
     9721 cgtagcacca ttattattct ttattcacgc agtacttgat ggtttatcat tcttaacatt
     9781 gtacttatta gatcttcatc taggttatac attctcaggt ggtttcatcg actacttctt
     9841 actcggtata ctacctaata agacacaatg gtggttagtc attcctgtag gtcttgtata
     9901 cgcagtcatt tactacttcg tattccgatt cctaattgta aaattaaaat acaaaacacc
     9961 aggtcgtgaa gataaacaat cacaagcggc tactgcttca gcaactgaat taccatatgc
    10021 agtattagaa gctatgggtg gcaaagcaaa cattaaacat ttagacgctt gtatcacacg
    10081 tctacgtgtt gaagttaacg acaaatctaa agttgatgtt cctggtttga aagatttagg
    10141 cgcatctggt gtattagaag tcggcaataa tatgcaagca atttttggtc caaaatctga
    10201 ccaaatcaaa catgaaatgc aacagattat gaatggtcaa gtagtagaaa accctactac
    10261 tatggaagac gataaagacg aaaccgttgt tgttgcagaa gataaatctg caacaagcga
    10321 attgagccat atcgtgcatg caccattaac tggtgaagta acaccattat cagaagtgcc
    10381 tgatcaagtg ttcagcgaaa aaatgatggg tgacggtatc gctattaaac cttcacaagg
    10441 tgaagttcgt gcaccattca acggtaaagt acaaatgatt ttcccaacaa aacatgcaat
    10501 tggtcttgta tcagatagtg gtttagaact attaatccac atcggtttag acactgttaa
    10561 attaaacgga gaaggcttta ctttacatgt tgaggaaggt caagaagtta aacaaggtga
    10621 tttattaatc aactttgatt tagactacat ccgcaatcat gcaaagagtg atattacgcc
    10681 tattatcgtt acacaaggaa acattacaaa ccttgatttt aaacaaggtg aacatggcaa
    10741 catttcattt ggcgatcaat tatttgaagc taaataatgc ttactataaa caggtgcgta
    10801 taccttcata aggtgacgcg cctgtttttt cattgcgatt gtgttttgca gcatcattga
    10861 tagttcgctc tccccttaaa ttttgaattt taaaatcatc aattaaatcc cccttcatac
    10921 tcatttccta aaaaatatta attgctcact attgttagcg ttttcacaac aaagtcaact
    10981 tccttgacct tacactatat tcgaggctat cattttaagt gtaaatatag agaaaaggtg
    11041 gtttttttat gaaacaacgc attggagctt acttaattga cgctattcat cgagcaggcg
    11101 tcgataaaat ttttggtgtt cctggtgatt ttaatctcgc ttttctagac gatattatca
    11161 gtaatcccaa tgtagattgg gtaggaaata caaatgaatt aaacgcaagt tacgcagcgg
    11221 acggttatgc ccgtcttaat ggactcgctg cattagttac tacatttggt gttggcgaat
    11281 taagtgccgt caacggtatc gcaggttcgt atgctgaacg catacctgtc attgcgatta
    11341 caggtgcgcc gacacgtgct gttgaacaag ccggtaaata tgtacatcac tcacttggtg
    11401 aaggtacatt tgacgactac cgaaaaatgt ttgcacatat aaccgttgca caaggttata
    11461 tcacacctga aaatgcagca actgaaatac cacgcttaat taatacagca atcgccgaaa
    11521 gacgcccagt tcatttacat ttaccaatcg atgtcgcaat ctctgaaatt gagataccga
    11581 caccattcga agtaacggca gccaaagata ctgatgcatc aacatatata gagttattag
    11641 tatctaaatt gcatcaatcg aagcagccta tcatcattac tggacatgaa attaacagtt
    11701 ttcacctcca tcaagaatta gaagattttg taaatcaaac acagatacca gtagcacaac
    11761 tttcattagg aaaaggtgct tttaatgagg aaaatccata ttatatgggt atttacgatg
    11821 ggaaaattgc cgaagataaa atacgagatt atgtggacaa cagcgattta attttaaata
    11881 ttggagccaa attaacagat tcagcaacag caggattttc ataccaattc aatatcgatg
    11941 atgtcgttat gttaaatcat cacaatatca aaattgatga tgttacaaat gatgaaatat
    12001 ctctatcatc attactacaa caattgaccg atatatcata tacgaataac gcatcgtttc
    12061 ctgcatatca tcgtccaaca tcacccgatt atactgttgg cacagaacca ttaacacaac
    12121 aaacttattt taaaatgatg caaaatttct taaaaccaaa tgatgtcatt attgctgatc
    12181 aaggtacatc attttttggt gcttatgatt tagcattata caaaagcaat acttttatag
    12241 ggcaaccgtt atggggttct atcggctata cattacctgc aacattaggt tcacaattag
    12301 cagacaaaga tcgtcgtaac ttattattaa ttggcgatgg ctcattgcaa ctaactgttc
    12361 aagctatttc aactatgatt agacagcata ttaaaccggt attatttgtg attaataatg
    12421 acggctatac ggtagaacga cttattcacg gcatgtatga accttataat gaaattcaca
    12481 tgtgggatta taaagcttta ccagctgtat ttggtggtaa aaatgttgaa attcatgacg
    12541 ttgaatcatc aaaagattta caagaggtat ttaatacaat taatggccat cccgatgtga
    12601 tgcattttgt cgaagtcaaa atgtctgtcg aagatgcacc gaagaaactg atcgatattg
    12661 ctaaagcttt ttcacaacaa aataaataat ttcatcgtat tcagggtata agtttaagcg
    12721 aatatattat taaatgaaaa ggactctgat ataagatgat taattttgat aaaaccgctt
    12781 tagtgttaat cgacctgcaa caaggtattc ttaaaatgga ttatgcgcca catacagctg
    12841 aaaatgttgt tcaaaatgct aacaaattaa ttgaagcttt tagaaaaaac aatggcttta
    12901 tcgcctttgt tcgcgtgaat ttctatgatg gtaaagatgc attgcaacca aatgcaatga
    12961 tctcattacc accaaaagaa ggcgatgact acagccattt ccattattta ctagacaaaa
    13021 gagatgacga ttttgtcata gataaacgac aatttagtgc atttgtagga acggatttag
    13081 acttacagtt gagacgtcga ggaattgata caattgttct tggcggtgta gcaacacatg
    13141 ttggtgtaga tacgacagca cgagacgcct atcaattaaa ctacgatcag tattttgtta
    13201 cagatatgat gagtgcacaa aacgaaacgc tacatcaatt cccaatagat aatgtattcc
    13261 cattgatggg acaaaccata accacaaacg acttgctaaa tatattgaac taaacattca
    13321 cttccccctt cgattatatt gaagggggta tctttatttc acaatgtatt aatacgtcgg
    13381 gttgtctaac ctccactata taaacatatt ctatatctgt taaaccgttc ttaacctatg
    13441 cccttactac ataaaaaaca gtatttattc cggaattttc aaaaaattta gtatttatta
    13501 caaaattatg tatcacttta ggtttaattt ttgatattat cttaattaag tagattttta
    13561 taagttctaa aaaggagaac aaatacatat atgaagaaga aactaacatt taaagaaaac
    13621 atgtttattg gttctatgtt atttggttta ttctttggtg ccggcaacct tatcttccca
    13681 atacacttgg gtcaagctgc tggttctaac gtttttatcg ctaacttagg atttttaatt
    13741 acagcaattg gcttaccatt tctaggtatc attgctattg gtatttcaaa gacatctggt
    13801 ttatttgaaa ttgcatcgcg tgttaataaa acatatgctt atattttcac gattgcctta
    13861 tatctagtta tcggaccatt tttcgcctta cctagactgg caactacatc atttgaaatt
    13921 gcattttcgc catttttatc accaaagcaa atcactttat atttatttat tttcagcttc
    13981 gtcttctttg tgattgcatg gttttttgcg agaaagccat caagaatttt agaatatatc
    14041 ggtaaatttt taaatccggt attcttagta ttattagcaa ttattttatt atttgctttt
    14101 atccatccat taggtggcat atctgatgca cctattagta aacaatatca atcaaacgcc
    14161 ttatttaacg gctttttaga tggatacaat accttagatg cgctagcgtc attggcattt
    14221 ggtatcatca ttgttgcaac gattaaaaag ttaggtatcg aaaatccaac tgatatcgct
    14281 aaagaaacaa ttaagtctgg tactatcagt atcattatga tggggatcat ttatacccta
    14341 ctagcaatca tgggtacatt aagtattggt catttcaaac ttagtgaaaa tggtggtatt
    14401 gccttagcgc aaattacgca atactactta ggtaactacg gtatcgtctt gttatcactc
    14461 atcgttatgg ttgcttgttt aaaaacagct atcggtttaa ttacggcatt ttcagaaaca
    14521 tttgaacacc ttttccctaa aatgaattac ctagcgattg caacagttgt gagctttatc
    14581 tcattcttat tcgcgaatgt tggcttaact aaaattatta tgtactcagt cccagtgtta
    14641 atgttcttat atccattagc aattgccctg attgtactaa cattatttag cagcaaattc
    14701 catcattcaa aaattattta tcaatgtacc attttcttta caatgattgc tgcattagta
    14761 gatggattaa aagctagtcc agagttcatt tcaagcacat cattctcaca aactttgatt
    14821 aatttcagcc aaaaatattt accattatca gacattggta tgggctgggt tgttctcagt
    14881 ttgattggtt tcattatcgg cttcattatt tataaaatta agcatcgtaa aattccacaa
    14941 gcataatact atgccacagt catatgttaa acatatgctt gtggcatttt ttattcatac
    15001 tacattaaac tgcactcgta tacatacata ttaatgatta tccacgaaaa atattagtac
    15061 tttcatttta cgaatcacat taatgcatac acaactttat ctttatatta ttaaatttac
    15121 atttgacact tatattgaac aactgtaata tattaatatt aattctttaa attgtataaa
    15181 tataaaggag ggcgaccgat gaattcaatc attgaattaa ctgattatta tagctctaat
    15241 aattatgcac cactgaagct tgtaattact gaagggaaag ggggcaaagt ttgggatact
    15301 gatggcaaac aatatataga ttgcatttcg ggtttttcag ttgcaaacca aggccattgt
    15361 catccaacaa ttgttaaagc gatgacagaa caagcttcaa agttgtctat catttcacgt
    15421 gtcctttata gtgacaatct cgggaaatgg gaagaaaaaa tttgtcatct tgctaagaaa
    15481 gacaaagtac tcccccttaa ctctggtact gaagctgttg aagcagccat taaaattgct
    15541 agaaaatggg gctctgacgt taaaggcatc actgacggac aagttgaaat catcgctatg
    15601 aataacaatt ttcacggtcg tacacttggc tcattatcac tatctaacca cgacgcatat
    15661 aaatcaggat ttcaccccct acttcaaggt actaaaacag tagattttgg agatattgag
    15721 caattaacac aagctatttc accaaataca gcagcaatta ttttagaacc aattcaaggt
    15781 gaaggtggcg ttaatatacc accgaaagga tatatccaag ctgtgcgtca actatgtgat
    15841 aaacatcaaa tattattgat tgcagatgaa attcaagttg gtcttggtag aactgggaaa
    15901 tggtttgcga tggaatggga gcaagtcgtt ccagacattt atattttagg taaggcattg
    15961 ggtggcggct tataccctgt atctgctgtg cttgcaaata atgatgtcat gcgagttcta
    16021 acaccaggta cacatggctc aacatttggt ggtaaccctt tagccattgc aatatcgacg
    16081 gcagcgcttg atgtacttaa agatgaacaa ctggttgaac gatcagaacg cttaggttca
    16141 tttttattaa aagcgttgct acaacttaaa catcctagta ttaaagaaat tagaggtcgt
    16201 ggtttattta ttggcatcga acttaacaca gatgccgcac ctttcgttga acaactgatt
    16261 aaacgtggca ttttatgcaa agacacgcat cgtactatca ttcgattgtc accacctcta
    16321 gtcattgata aagaggaaat caatcaaatt gttgcagctt ttcaagacgt ttttaaaaat
    16381 taacaattaa tcatttatat atgatatagg agggattcat gatgattaaa gtaggtatcg
    16441 ttggcggtag cggttatggc gcaattgaat taattcgatt attacaaaca catcctcatg
    16501 taacgattgc acacatctac tcacattcaa aagtagatga gccgttgaaa ttaacatttc
    16561 cacatttaca acatattatg caacatttcg aagcacttac agtggacaat aatgactgtg
    16621 atgtaatttt ctttgctaca ccagcacccg tgagtaaaac atgtatccct cctttagtag
    16681 aaaaaggaat tcatgttatt gatttatctg gtgcattcag aattaaaaac cgtgaaatat
    16741 atgaagcata ttacaaagaa actgctgcag cacaagatga tttgaatcat gctatttaca
    16801 gcatttcaga atggcaatct ttagataaca atggaacgaa actcatttct aaccctggct
    16861 gtttccctac agcaacgtta ttagcattac atccacttat tagcgaaaaa attgtagatt
    16921 tgtcatctat tattattgat gctaagaccg gcgtgtcagg tgctggtcgt tcattatcac
    16981 aacgtgttca tttttcagaa atgaatgaaa atctaagcgc ttatgcaatc ggaaaccata
    17041 aacacaaacc ggaaatcgag caatatttat ctatcattgc gggtcaagat gtatcagtca
    17101 tatttacacc acatctcgta ccaatggcac gaggtatttt atcaacaata tatgtcaaat
    17161 tatcatctga atatacgact gaatcattac ataaattaat gacctcttat tatgctaatc
    17221 agccatttgt cagaattaga gatattggga cttttccaac cacaaaagaa gtactcggta
    17281 gtaactactg cgatatcggt atctatgtag atgaaacaac gcaaacagca attttagtat
    17341 cagtgattga taaccttgtc aaaggggcaa gtggtcaagc cattcaaaac ttaaatatat
    17401 tatatgattt tgaagttacg actggcctaa aacaatcacc agtttatcca taaggggtgt
    17461 tagaatgaaa catcaagaaa cgacatcaca acaacataac ttttcaatta ttaaacatgg
    17521 cgatatcagt acacctcaag gcttcacggc cggtggtatg cacatcggtt tacgcgctaa
    17581 caaaaaagac tttggctgga tttactcatc gtctttagca agttcagctg ccgtatatac
    17641 tttaaaccaa tttaaagctg caccacttat tgtcactgaa gacactttac aaaagtctaa
    17701 agggaaatta caagcacttg ttgttaattc agctaatgca aattcttgca caggtcagca
    17761 aggtatcgat gatgcacaac aaactcaaac atgggttgct caacaacttc aaataccatc
    17821 tgagcatgtt gctgttgctt caactggtgt tattggagaa tatttgccaa tggataaaat
    17881 taaaactggt acagaacata ttaatgatgc taattttgca acgccaggtg cgtttaacga
    17941 ggcaatttta acaactgata catgtacaaa gcatatcgct gtatcactaa atattgatgg
    18001 taaaaccatt acaattggtg gcagtgctaa aggatcaggt atgattcacc caaatatggc
    18061 taccatgctt gcttttataa caaccgatgc atcaattgaa tcgaatacac ttcatcaatt
    18121 attaaaatct tcgactgacc atacatttaa tatgattact gttgatggcg atacaagtac
    18181 aaatgacatg gtattagtca tggcaaatca acaagttgaa caccaaatac ttaatcaaga
    18241 ccatccacaa tgggaaacat ttgttgatgc cttcaatttt gtctgtacat ttttagctaa
    18301 agctatagcc agagatggcg aaggcgcaac aaagttaatc agtgtcaatg tttcaggcgc
    18361 taaatcaata tcagacgcaa gaaaaatcgg taaaactatc gtaagttcaa atctagtaaa
    18421 atcagctatt tttggcgaag atgccaattt tggtcgaatc attacagcta ttggctatag
    18481 cggatgtgaa attgatccta actgcacata tgttcaactg aaccaaatac ctgtcgtgga
    18541 taaaggtatg gctgtactat ttgatgagca agctatgtcg aatacattaa ctcatgaaaa
    18601 tgtcacaatt gacgttcagc ttggtttggg taacgctgca gcgactgcat acggttgtga
    18661 tttatcctat gattatgtgc gtattaacgc atcatatcga acataaggtg gtgttggtta
    18721 gatgaaattt attgtcatta aaattggtgg cagtacacta agtgacatgc atccatcaat
    18781 tattaacaac attaagcatt tacgatcaaa caacatctac cccattatcg ttcacggcgg
    18841 tggcccattt attaatgaag cattatcaaa ccagcaaatc gagccacact ttgttaatgg
    18901 cctaagagtg actgataaag caaccatgac tattactaaa cacacgctca ttgcagacgt
    18961 taacactgca ttagtagctc aatttaacca gcagcaatgt tctgcaatag gcttatgtgg
    19021 tttggatgct caattatttg aaattaaacg ttttgatcaa caatatggat atgtcggggt
    19081 tccaaccact ttaaatatag actcattgtc ttatttatgt actaaatttg tacctatcat
    19141 caattcgatt ggtttcaata accatgatgg agaattttac aacataaatg cagacacact
    19201 cgcctatttt attgcagcat cattagaagc acccatttat gtactaagta atattgctgg
    19261 cgtgctaatc aacgatgttg ttatacctca actaccctta gccgatatta atcagtatat
    19321 tgagcatggt gatatttatg gaggtatgat tcccaaagtg cttgatgcta aaaatgctat
    19381 taaaaatggc tgtcctaaag ttatcattgc atcaggaaac aagccaaata ttatcgaagc
    19441 tatttacaat aatgattttg ttgggacaac gatactaaaa tcatcagtat gactttgaat
    19501 atgaccactc aatcttgtat ttttcggctt cgaaacatag ttgtacctaa tttgtttaat
    19561 gattatgcag cgtcaccttt tcacctgtct catttcacaa aagaattctt agaatacagt
    19621 atgatacaat atgagatgaa aaacactata agtgatttta aaatttgtaa ctcgaaatat
    19681 tttatatttt aaaagttttt cattgtgatt attttcacaa aatactataa tgaggatagt
    19741 aaatagagag gagtccttaa gttgacaaaa cgacaaatgg gtatattcat ttatgctgga
    19801 attatcggtg gcttgttatc tggaattgta aaattaggtt gggaggtcat gtttccacct
    19861 cgcacaccag aacgtaatgc aacgaaccca cctcaagagt tattgcaaca attaggattt
    19921 agtagtgagt ttacgcatca aacatataca ttttcaaata tgcaattgcc ttgggtaagc
    19981 tttattgtcc actttagttt ttctatcgtc attgcaatta tttactgcat attagttaaa
    20041 aaatacgctt acttagcaat gggacaaggt gctgtttttg gtattgctat ttgggtatta
    20101 ttccacctta tcattatgcc aatcatgcat actgtacctg ctgtgtggga tcaaccattc
    20161 caagagcatc tgtcagaatt ctttggccat atcgtctgga tgatgacaat tgaattagtg
    20221 cgacaacatt ttgtctatcg ctataaatta aattaataca ctgactaaca ttaacgtgag
    20281 ttttaaatca tcgtttgagt atgatgattg atgctcacgt tattttatta actgacatga
    20341 tataattcta gccaacttac atgagcatta aagtctcaaa tgcgtcgtaa caagccatta
    20401 tttttggcaa tttcaatatt gctcagtata ttttcacctt atcacttact ttaatctcgt
    20461 catgattttg aatgatgcca tcgtgtattc acctttcatt tttccaataa aaaaacatct
    20521 aacagtaaac atttaggcag tatagtttaa aatcactgcc aaataaaact gttagacgcc
    20581 atgtttaact actcaataac tgaaatacag acactttttt ataaccccag ggtgcctgtc
    20641 ctaagaaaca tacctgtacc ataaactgat caaaaataaa ttgtttgaac ttcacttcac
    20701 gtgattgata aaagtgtgat tgcgtcatat cataaatgtc caatcctttg attaaacctt
    20761 caccaatcaa ttttgtaaaa ctttcttttt gtgtccatat ttgataaaaa tcatttaaac
    20821 tacatatttg atgtgcttcg tttgtagaga aacacgtcac taatgtacgc cagtctaaac
    20881 gttgtgatat cttttcgata tcaataccaa ctggttcttt atcgacaaca cacacgatat
    20941 aaggataact atatgataag ctcacataga tgggctgtcc atcacgattg tgttgaacta
    21001 tatctgcctt acctcgtggc gaaatgtgat aatgccattc atgtggtaat aaccctgtgt
    21061 catgttgaat tccatattgc actaaaatat ctcccaatct gtgcatgagt ttatcttgat
    21121 tgtatctata gttgactgta cgcggttttt tatatgacca acgactttgt gatattaatt
    21181 cttcaatact tttcaagtta ctctgtaatt gcattacaaa tactttcata actttcccta
    21241 cttacttatt gaatattgtt ttgatatatt gtgcccaatg atacagccaa ttgttagtta
    21301 tcgttggcca tttttcactg atgtgatcca ttatttttaa tgttaatttt gtatctatca
    21361 ttgctagttg ttgttcacgg tcaacactag ttaatccaat cgtttcgtac atgtcttgtt
    21421 tctgtaaaat ttcattaaat gattcatcgc tgacgagttc aatttctttg cacttaacac
    21481 attctaataa agatttcacc ggcattttat taggtgataa cacatggtaa atgatttgtg
    21541 gcgtgttgac ctgtgctaat gcgacaattt gtcttgcagt cgtatccaca aaagaaaaat
    21601 ctacaggcat ttcagcgagg ctaaccccga tacaatccag ttgtaacaaa tcattcatta
    21661 ccattgaaaa acggttagtc tttatatttc tcatatgcca tcttccattg tatggactcg
    21721 tcaaattacc aacacgtaca attcgaccat ctaagccatt ttttacagct tctaatactt
    21781 ttaattcact ataaaattta ctccgtgtat atggtgatgt tagtagttgt cctttataga
    21841 catccgcttc tgaaaatgtc acatcttctg tatctatatc aaaataagtt cccacactta
    21901 tcgtagacac atatattaac cttgcatgat gttgttgtgc caaacgtatg acatcaacag
    21961 taccttgaac atttactttt tcaaattcat catcatcacc aaagtgatct gtacgagcac
    22021 ctgcatgaat aattgtatcc atgttttctg gtaaaacaac atcatccata cactcgaaat
    22081 caccaacaat gacttcaatg tttgataaca tcatttcaac cgtctcttct gaaaaataat
    22141 catttaaatt cgtcatcaac ttataccatg ctatttcctc attatcagca cgtatgaaac
    22201 aataaatgcg atgactgtat ccttgtagtg cttcaatcag ataagcacct aaaaaccctg
    22261 tagcgccagt caatagtgtt tttcctagag gtcgatgact tagactatcc tctaaaatac
    22321 ccaaattata acgagacata acaatctttt gtaattccga aagattatcc ggtaatgcaa
    22381 ctaatgattg ttgattttgg tgcatataat taacaatctg tcgcacggtt ttatattgat
    22441 ataatgtctg cattgaaata tgatggccaa atcgttttaa atgcgagaca actaacatcg
    22501 cctctaatga gttaccacca agttcaaaga aatcatcgtc aacaccgaca tcattttgtt
    22561 tcaatacctc tccgaataca tcaacaaatg tctgctcaat ttcattagat ggttcgctat
    22621 atactttatt agactgttgt ataggtgatg gatttggcaa acgcgtagta tccaccttgt
    22681 cattcgtggt taatggtata caatcgatat acgttatagt cttaggaatc atatacttag
    22741 gcagatgatc atttaaatat tgcttcaaat cctgctccat ttgttgttct ccgacataat
    22801 aagcattcaa tatatcatgc gtatcaaagt gactaactgt gacaacacaa tcagatatac
    22861 cacgaatagc taatattgca ttttcaattt catcaagttc aatacggtac ccgttaactt
    22921 tcacttgttt atctattctt cccaaaaatt caatttgacc atcagatgta taacgtgcta
    22981 aatcaccact atgatacaac tttcctttac caaatggatt attttgccat ttatcagcca
    23041 ttaattccgg acgattaata tatcctatcg ctaaactatc acctgcaata cacaactcgc
    23101 ctggcatacc aataccgcat aacaaaccat ctgacataat atacacttgg atgttagata
    23161 agggtttgcc aattggaatc gtctcaggta tcaaatcacc acattgatgt gaccaatacg
    23221 atgtgatgac tgttgactca gatggtccat aggcattgaa atacgtgcca caatgcttct
    23281 caatatattt aacaaaggat gccgtactag ttgccccgcc tgtaatcaat ttttcaatat
    23341 aaaagtcttc cataacacta cacatctgta atggaatcga cgccaccgtc acacgatgct
    23401 tattaatgag ttgttgtaac tgttctggat taacacgttc ctctctatct ggaatcacaa
    23461 gcgtatgacc atttaacaaa caacaataaa tctccataac tgatgcatca aaaacaatat
    23521 ttgcatgttg caaaaatact tcattgtcgc ctaattgcaa ttcagttgac catgcatgca
    23581 ctaaattcaa caaatttcgt tgtcgtatgg caaccccttt aggcatcccg gtcgtaccag
    23641 atgtgtaaat agcatacatc tcattatcta acatcgctgt gttttcgagt tgatcgccat
    23701 gtaaatcatc atattgttca ttttcctttg attcaacaaa acctttagca ttttccaatt
    23761 gaatgactgg taatgtcgtt tcaatttcaa cgccgtaaga catgactgca gttactttag
    23821 catcctccaa aattgcacct tgtcgctttt tcggaaaatc aatatcgata ggtatataag
    23881 atgcacctac tttaactgtc gccaacatcg ccgcaatcat ttcaaaacta cgttctgtta
    23941 ttaaagcaac tggttgaccc ttgcccacac catttgatag gagcatgtgc gcaatagcat
    24001 ctacatagtt gtgtaatgtt tcatacgtca ttgtcaaatc attcatgact agcgcaacat
    24061 gattaccttg tcgtgagaca acttcatcaa agtaacttat gatagattta tttcccggga
    24121 cattaagcat tcgatcgtta acatgcgtat tgacccaatt taaaagttcc tccgtgccgt
    24181 ttggtatatc acgaatttgt agtgtatctt gatgcttcaa aatataatca atcataatca
    24241 tacattgatt acccatgtga cgaactgttt ctgagtgata taaatcggta ttatactcga
    24301 tattgattgt atagtcatcg caatcttctt caatgatgaa agataaatca aatttcgccg
    24361 tcaccgattt gggttgaatg tgtgttaatt tactatgccc aaaatgagca tgattcgttt
    24421 cattgttttg tagtactaac atgacatcaa ataatggatt ccgtgaggca tcatgtgatt
    24481 gatctaagtc atttactaaa cattcgaatg ggtattcttg atgctcgtat gcctccaaac
    24541 tcatttcctt aacctcttgt aaaaactgtg tccacatttt atcaagtgat ggttgccctc
    24601 tatataccaa cgtattagca aacatgccta gcatttgctc cgtgccttta tgcatacgcg
    24661 cactcatgac actaccgaca acaacatcat cttttcgagc atatctgctt aacaacgtca
    24721 tgaccacact cataaagaac ataaaatcag taatttgatg cttttctaca tacttttgaa
    24781 gtagctgtct catttgttga ttcattgtaa atgacatcat tgctccattt gtcgttttaa
    24841 tatttggtct aacatagtct gtcggtaagc ttaaaatagg tacttcatct ttgaattgag
    24901 ataaccaata ttgtctatgt ttcgtcatat cacgatgcga catccattca ctatagtctt
    24961 tatattgcaa tttaagtggt aacaataatt tatgttgata taatgcgtta agatcattca
    25021 ttaattgtat attactcata ccatcattaa taatatgatg tgtatctata aacaggtatg
    25081 catgtaatgg acttcgaatg tatctcactc taatttggct tggcttttcc aaattaaaag
    25141 gtgccacaaa ttggcgcatg atttcttgtt catccgtaaa atgcgtgtta acttctttaa
    25201 agtcagctac aacatctgcc acaatacgtt gtcgaacctc atcatctaca acaatatatt
    25261 gtgttcgtag aatctcatgt cgcgctatca aatgctgaac tgcttgtcgc aattgagcta
    25321 cattaagttc tgatgataac cgccataaaa aaggtatgtt atacaccgta tctttatggt
    25381 ttgatttcca taataaatac attcgctttt gtgcagagct caacacataa tcatctttaa
    25441 ctatagcttc aggaatcact tcatagtttt gttcttgaac cttagcaatc gcttgcgcta
    25501 gttcaaatac agttggcttt tgtaataaat caccaatttg taatcgtttc ccagtagatg
    25561 cctctatccg attcaccact aacgttgctt ttaaggaatg gccacctaat tcaaagaaat
    25621 tatcatgaat acctacttga ttcacatgta aaatatctgc aaaaatttgg catagcaagt
    25681 gttcggtatc tgtactcggt gctacatagg catccgtatc gacatagtcc atgataggca
    25741 atgccttctt atctaatttc ccattaatag taataggaat ttgctcaata tgcataaaat
    25801 taactggtat catgtactca ggtaaggtca tacgtaattg tgatttaatc ttattatgtg
    25861 ataatgtatg catcgcttca taataagcaa cgatatactg gtcttgatca tgattttgaa
    25921 caataacaac agctttatta ataccttgta tacgctcgag cgcatgctca acctctgaca
    25981 actcaatcct aaatcctcga atcttaactt gtttgtcctt tcgatataaa taatctatgt
    26041 tgccatcggg taacaaacgt acgatatcac cacttctata catcagctga tttatatttg
    26101 aatctttgat aaatttatct gctgtcaatt ctggctgatt taaataacct gcagccaacc
    26161 caaagccact tgtacataat tctccaggaa taccaacgcc acaccgacgc tcgccttgca
    26221 tgatataaac atgagtacca agaatcggtt taccaatagg aatacgattt ggaactttgt
    26281 taggtatatt atacgtcgtt gtaaatgttg tattttcagt tggtccataa ccattaataa
    26341 tttgaggatg cttcggtctt tgattaagca aatccaccca cttagcattc aatacttctc
    26401 caccaattaa taaatacttt aacggtacca atacttctat tcgttcacta gcaatctgat
    26461 taaataatga ggaggttaac cacatagtat tgacgtcatt ttcattgatt aattgttcta
    26521 acgctattgg atttaataat tgttctgttt tagcaacaat cagttttcca ccatttagca
    26581 atgcaccata tatttcaaat gttgcagcgt caaaggctat agttcctgat aacaaaatcg
    26641 tcgtcttttc atttaatggt acataatgat tttgatggac caagcgaaca atacctcggt
    26701 gcggaattag tgtcccttta gggttaccag ttgtccccga cgtgtaaata acatatgcat
    26761 gatcttctaa cgtgttacat ttagaaagat tatcaatatt tttccacgct atcttgttca
    26821 aatcaatgtg attaatatct cgtttaccat tttcatatac tacttgatat gtgataacaa
    26881 ctttaggcgt tgcatctttt aaaatgtact cctgacgatc acttggatag ttcggatcaa
    26941 ttggcacgta agccccacca gctttcaaca cacctatcat cgctactatc atctcaatac
    27001 ttttttcagc tatgacagcg acacgatcat taggttcaac accatactgg tttctcaaac
    27061 gttgtgctaa atcattcgcg cgtgcattca atgtttgata tgttataaac actccgtcaa
    27121 attgcacagc gacataattc ggtgttgctt caacttgttg ctcaaataag gtaacaactg
    27181 tttgcgcatc atctatctca ggtaaactta aattgatatc gtcatataat tgaatatcac
    27241 gttctgtcat caaattaagt tcatctacag ttgttcgttt atttccattt tctttagtaa
    27301 tttgcaaata aatattacga actaagtcac tcagcgtctc gattgagagc aaatcatatg
    27361 ctgcactgtt atatataatt ttgaacccgt gttgatgtgg aaaaatttca atatccgcta
    27421 aagatgtatg tgcatcatgt atttgatgta catcctcaat aacatcatta caacaagaca
    27481 tcatttgatg ataacaatga aaaatagtct ctaatgaaag cgaagacttc gcgcactgta
    27541 attgcgacat attttgcaac acacttttat taaaatctgt tgtaaagcgt tgacatacat
    27601 cttttgcatc gattgttaac gttaatggca caatatttcc gtgtaaatca tttggtaaat
    27661 gtgatggtac atgtatacct aatgtgacat catgttgttg actcattata tgattagcta
    27721 aatacacact aacagccaac gatgccatat ctattgatgt catatcatca atcaaatacg
    27781 tttgatataa agcttgctca aatggatgct taattggtaa ataactatcg atatggatgt
    27841 cagagttatt ctctagccga aaatagtttg agtctaatgc tatatgcgat gcatcttgat
    27901 tgtctttatc atcatttcta tttaaatgtg catgctgtcg agtattgtta ataacagtat
    27961 tgccacgata tgcattgcat aaatcatcaa gaaaaatatc aatttgacta tcatcgaaaa
    28021 tggacacatg aaaatctaat aatatatatg cagcatcagc gaactgaaac aatttaaatt
    28081 tgaataaagg tgaatcatta aaatggtaag tacttaattc ttgcttaaaa aaagcttcta
    28141 aatcatagtt tgcggaagaa gatggaactt gttttatctc aataaaaggc agaaattcat
    28201 gaagtatcat tgttaaattg tcatcggtag taacatcaaa aaaatgtctt atagatgcat
    28261 gttgttgcac aattgtcgat aatgcataca tcattttagt agcttcaata tttttagcga
    28321 gtttaaccca atacgcatta cggtgtgtcg ttgattctgt attatttttg tatatacgaa
    28381 aatattcctg ttgaaatctc aaattgccca taatcataaa aagtccttct ttcatatcat
    28441 aatactcatt acttactgaa attgcatgat gatatgataa ccgacgaaat gttaattaac
    28501 tcgttatgta atgattaata taaaacacca ttcgcaacat atgagcgata tattctaccc
    28561 taaaatacat cttgtatcat cgttacaatt ggtatatttt tcaatgtaaa ttacatacat
    28621 attcgataac tagcacacta caactcgtta atcactttct cttattcata tctcattgca
    28681 agttcaatat tgttgttaca aaatatccat gaagcaagtt tatattaaac aaacaactcg
    28741 cataaaacaa ttgttatcct taaattttaa caaattctta ataaatttat ctctatttta
    28801 attacgacca aattaatagg ttttcaatat aaaaagatgc atagaataaa tatttaaata
    28861 aattcaattt gtattcactt gtttttgtcc cccgaataca ccagcaacaa gcatgctagc
    28921 accaattgtt aaaactataa acatatacag tcccatttgt aatgtcgtta agaaagcacc
    28981 caacacaatc cctaacctag ctagtgtttc tgaaaaatga atgcctaatg cattaactgc
    29041 actatatgtt cctcttttag ctttaggaat aattttaaag cgttgttctg aaactatagg
    29101 cgaataaata atttcaccta cagtcgcaat tatcataaaa acaactaata agccaaactg
    29161 attaagatag gttagaccac tatagccaac aatataaatc agcaaaccag tgattaaagc
    29221 ttttttaaaa tctattttca atacaatttt cgagattgaa tacgtgagta aaatgacgac
    29281 gaccgtatta atcattagca agattgctaa catcttagca cctgtaatat catatgaacc
    29341 tatacttatt gtttcaaact gatcctttag tctaatagca atatatgagg agattgaaaa
    29401 ttcacccatc atgatgatac tgaaccccga aatcaataac atataattac ggtctttcaa
    29461 aactaattta taactgcgaa atatattcat tatttgtaat ttttgataac gacttgcatg
    29521 tttcttgtca tcactttgct ttacttgatt tcggtcttga ggtaaccaaa tatataaaat
    29581 aaacagtaca attaaaaata tacaagctgc tattaagaaa agcagtaaca tactgtagcc
    29641 atacatcaag ccacctaaca atgctccaat agctaccgat aagttcgtca tccaatagct
    29701 aatcttgtaa atataatgtt ccacatcttc ggtaattgca tccataatta acgtgtccat
    29761 aactggaaat tgcaatcccc aaacaattgt aaatatggcg tatgcaacac aaaaaccaat
    29821 aatttgccac aattgatgtg acccaaatat gcccatgaac acaagcatta tcaccatcgt
    29881 cgcttgataa ataagtacta gcaacttttt cggaaatatc tcaataaggt aaccagatat
    29941 aatagacaat ggaaatttta gaaccactaa accaacaagg tatataccga caattgattg
    30001 acttaacata tctgttaaat atagtgctat aaacggtata aatgctgtcg taataattag
    30061 ctgtaaaaaa ttgctaatca atcgtacttt caaagtcgta cttaaacgtt tcattgataa
    30121 ctacacccct atccacatat atgcacacta ccatttatat aaaatctatt atttgattat
    30181 acgttgaagc aaagaatttt tagttataca aagttattta acaaacaaat gtatcgactt
    30241 tacaatgcat cttgcatata atgcctcaaa aaaattagca ctatatgact atatattatt
    30301 ccaaatgagt gaaatttatc aacatttgcg taagtgaaca catatgtgcg taaacgttaa
    30361 atcattgtcc taacatagcc gtgactatac ttagatgtaa tcataaaaaa actactgcca
    30421 tctttacaac agcagtagta cttatatgat tcatttatat aaaattgaaa gcgttaatcg
    30481 ctagcccagc atccttattc tatttagctg tataactttt actagcaata cgaccacttg
    30541 caacaataat atctttatct tggaaaggtt catgattgaa gaaacgctct aaaatatctt
    30601 taactccatc ttcaatacgt ttttgtgctt ctaaagtcat acctgaatag tgaactgtca
    30661 tagcatttct aggcattgtt ctccatggat gatcagcagg tgcaggttgt ggataccaaa
    30721 catcaccagc atatccttgt aaatgctcag atgctaacgc ttcaactaac gcatcgcgat
    30781 ttacaatttt accacgtgca gtattcacta aataactgtg ttttttcata cgacttaaaa
    30841 catctttatc aaataagtta tcagtttctg gtgttaatgg tgcatgaatt gtaatcgcat
    30901 cacttgttga aacaagttca tcaaagctta caaatttaga caatttatgg tcttgttgat
    30961 tgattggatc atagtgttgt aatgttacat taaatggcgc taatctttca gcaacaagtt
    31021 gtccaattcg tccaaatcca aaaataccaa ttgttttgtg ttgtaattca tgcgcatgat
    31081 tacctacttg agacaagttc cattcacctt ctactgattg acgatgacct tcttcatagt
    31141 ttctaagaag tattaataaa tccataactg catgttctgc cacactaact gtattacttc
    31201 ctgtaacttc aacgacacca atgttatgtt cacttgctgc cgctaaatct acatggtcag
    31261 atcctacacc tgctgtaatt gctaatttca agttcggtgc tttttcaata cgttcacgag
    31321 tcatatatgc aggataaaat ggcgcactaa taatcacatc catatctggt aaatgtttat
    31381 ctaagtcttc accattatct gctaatataa tgaactcttg tcctctttcc tctaaaaatg
    31441 tttttaatcc taatgctttt ttagtattaa gtaattgatt gtcttgacct tctactgctt
    31501 ctggaaataa cgctacgatt ttcatatcca tctctcctaa atgataaatt ttgacttcct
    31561 taaagtacgt gttgcatgtt gcttgtttta aaaatatgac aaaaaaaacg gcactgtttg
    31621 acataccacc cccttgaatt attgttaaat tcaaagcgaa gttagcaaac agcaccgcgt
    31681 accttacacc agcaacactt attctattga cttatcgaaa acaaatattg aaatgacttc
    31741 atcatcattc aaatcaatat ctgtaaataa ttttacaaac ttagcgccta caacactttc
    31801 catctcattt acatctattt ctttatacat ctgtttaatc ttctctgtgc gtgtataatg
    31861 gagcatcgat tcattgcgtt tgtcgtttag gtaaaaactc tcaactttac tcaaaacacc
    31921 tgtcatatgt gcaatcgccc aattatcttt aaacgacact cgaatactgt tgggtccttt
    31981 acccacgtat tctttacgat aagatcttac tagattcgag aattgttgtt ctttagtatg
    32041 cgtatctata gtattcacct tctctaaaat aagaagttca ttaattgttc acattttcat
    32101 tatacacctt attaacactt tttcaatgat aacgattatt attacgaaat gtatattttc
    32161 ctagttaaaa ggatataaca aattaacaga aaatgtttat aaactaagat ttatattatt
    32221 ttaaaaattg tttcaacaag cgcatctaaa caacaaatca gcatatcata cttacccctt
    32281 caactctgcc tcaagtttac ctaaatgtct taatgttggc gttaatgccg caaagaagaa
    32341 tccttctttt aatttacgta cttgtggcga atatggtgtg tacgctcttg aaccaccatt
    32401 gacaacagaa gcttgattta catctaacaa taagtagcca atgtccttct tcaatgatat
    32461 taattcattt aaatgtgaag ttaagttacc gtcatctaat attgcataat attcctctct
    32521 aagttgacga tagcgttttt taaaaccttc aacatcatac tctaaatatt gatttattcc
    32581 gttttgcgca tttgaaaatg catcaattaa ctctaaagaa cttttaattg agcctaatcc
    32641 tattggaatt tggtaagcaa taaattgcgg gcgaatagtt gccgcaaact gcttcgcatc
    32701 atgcgtgata atttgtgatt gtggcactgc gacttgattc aaagtaattt gatacgttgc
    32761 tgagccgttt acccctaaaa aatttgtttt ttcaacaagc gtgataccat cttgattggc
    32821 acgtagaatg aacatgacaa attcatctga tgattcatgc ttcgaaatcg caccaaaata
    32881 atggtcttct tgaatattac ttacagctgg catacgtcca ctgacaacca attgtccatc
    32941 aacataagtg tgttcaaggt tcaacttttc taaatcatta aatgacttca tcggattaga
    33001 caatccggta gcacctaata tttctccaga taacaattgc tgttgtaagt cattatttaa
    33061 atgtggctgt gtggcatttt ctaaatacgt tgaaaaagcc aattggcacc ataaacaaaa
    33121 tcctgttgtc aaacaagact gcgatacagc ttcgattact tcagcatttt ttctcagtgt
    33181 cgcttcacca aaatagccat ctacaaataa ttgttggata aatgttctcg gataatacgt
    33241 cccttcatca acttctacta aatgaggatc taattgttct ttgataagtg tttctaaagt
    33301 catcatcatc tctccttatt caccaaatct tctaagtata aactgctcaa tataactaat
    33361 gaatcgatca ataataaaac caaataatcc gataaagaat attgctgcta aaacatcttc
    33421 taagttcaac atatttcgtg catcaacgat taaaaaacct aatcccgatt gtgcaccaat
    33481 catttcacca gaaactaaaa atatccaact tgttcctacc gccatatgta tcccagccat
    33541 gatttgttta aatgccccgg gaaataatat attgcgatac aatgaccacc cagttaaatt
    33601 taaatttgcc gctattttta aatattgagg ttcaatgtct ctaacgcctt taatggtatt
    33661 gaacacaatt ggaaaaaaag cagcgataaa aataatcgca atcgctggca aactaccaat
    33721 accaaaccat agaacaacaa atggtgccca agctatcgga gatatcggcc taatcaattg
    33781 aaatagcggt tcgatagcgt tgtatagcca acgattcctt ccaagcaaga agcccaatgg
    33841 aatagcaacc aacaatgcga caacaaagcc cgctacaaat ctccataaac taattgctaa
    33901 atgttggaaa atttctccag taacaatgaa agtccatatg ctttttccta caagagcagg
    33961 accaggtaac aatacaggtt ggtaatgccc aataataatg accatttccc aaatgcctaa
    34021 gaaaataata aatgtgataa taggtaatat aattttatta attgtgagac gtgtcatgaa
    34081 cgtgatgcct ccttatacaa tgacggttca acaaagtcat catatgcagg tggattaaac
    34141 aaatgatgtt gttttaccaa tgtcgtaatt tcttgatatc cggatggctt aattgttaaa
    34201 tcaccatagg atgtccatgc cgctgactgt gttaaaacgt cacgactttg tttaaaatga
    34261 tgcgtcataa tgtctacact ttgcttgcga tcattcattt taaagccaga ctttttataa
    34321 tcttgtacaa atgcttgcgc tacagccttg tgttgatcaa gcaattcgcc tctcagtact
    34381 agcacacagc aatacgcatc aggtataacg tcatcaccat gtttcaaagt cttacctttg
    34441 cctaactttt cccccagtgc accgaatggt tcggctacag aataccctgt aattctgtgt
    34501 tcacttaatg cggctggcat ttctgctggc gacatttcat gatagctaaa atgccccggt
    34561 ttaatcttta attgttctcg taatgcttca agtaagagat aatgtgttga ataacgatgc
    34621 ggtataccaa aatggtaatc atcgccatta ttattaaatt catttaagtg catacctttt
    34681 tgtcccataa tgacattgcc ttcatgatgc cccaatgcca cagcttttat attcgagccc
    34741 ttctgctttg atttcatcgc gagctctatt aaagttgatg caccatcaat acgaccactg
    34801 tttaatgcgt ccattaaatc tggccaatta ttgaatttaa ctaattctaa tttatatttc
    34861 ggatgattgt gttgtgataa taatttttta gtcatcatca aattagctga atgtgtaatc
    34921 ggcaaatatc caattttaat cacttgctga tgttgggcat tattagacgg ttctttagac
    34981 gccctttgcc aatcacatcc tgtaattata aagattccaa tgatgacgat tatgcttaac
    35041 cttttcatcg tcactcactc cttataaata atatccaggt tcaacttgat gatgattcaa
    35101 tgcaaatgtt tccataattt cattacgaat cttaagtagg tggctatcat tacgactgcg
    35161 tggatgtgat gctgtaattt catattgaga aataatattg cacccttcac ctaacagaac
    35221 aatgcggtcg gaaagataaa tagcttcatc aatatcgtga gcgactaaga taatagttga
    35281 ttccgtttta tgttttagtt gtactagttg atcctgaagt ttatagcgtg taaatgcatc
    35341 taatgcacct aatggctcat ccatcaatat aacgttaggc ttatgcacat gcgctcgaca
    35401 tagtgccaca cgttgtttca tacccccgga cagttgctcg ggaaaatgct ttcctctgcc
    35461 ttctaaatca actaatttaa gctgtgcgtt aatctcttca tcactaattt tctgttggaa
    35521 tccaatccta atgttgtcat taatcgtttt ccatggcagc agattatgat gttgaaatag
    35581 cattaaacaa tctggagacg gctgttgttt aatttcgtta tcaataatga cacgaccaga
    35641 tgatggatga ataaatccac cgatgatatt gagtaaagta gacttcccgc aaccactttt
    35701 ccctataaaa gtgactattt ctcccttgct aatgtccaaa ttaaagttat gaattacttt
    35761 atgtgatcca aagtgatgtt gtaattgttg tattttaatc atgacaagcc cccttttaat
    35821 ccctaccatt ttaataagaa ttaaatgtat atctaaaggg ctatgccaaa cacagccctt
    35881 aaatcgtgaa ttgttatatc tatagatgat tatggtttgg tatgagtact cttataacat
    35941 aagcgttatt caattaacga cgttatatta atttcagctc tactaaattt caataaaatg
    36001 attgttttag acaaaacttg catttagttc attagtttcg tcatatcaac tacttacatt
    36061 tatagcattg agctattcta attaactgca atcgaaatcc acgccaaacc tatatacata
    36121 tatatcatca acttgttgtc ttattaaagt ttttcgatgc catcaatcgg tgtatcaact
    36181 gctttgcgcg cgcgacgtac cgcttcttca tcaggtgcgt tgtataaaca aatacattta
    36241 gacatatctt cacatacata tgtgcgttta aattcaactt ctggcacttc ttcataatga
    36301 acagaatttt tctttttacg tgctaaatat tgatccatcg taatgccttc cggaatgttc
    36361 caagttacaa ggtaatcaac atgtgcatca ccatttttta cttcatctaa atctttacca
    36421 actaagcgta cttctttgac taattgaatg ctaatacctg cttctgttaa aaattgttta
    36481 gcttctgctt cgtcatttgc ttccacaata aaataaccat gagttaaatt ttcagtagct
    36541 tgtacttcaa ttaatgttgg aattgtcttc gtagatagtg atgatgcctt tgcttctagt
    36601 tcttctttcg ttgatgcaaa atcaagattg ttagcttcta ataaaaataa tgtcattaat
    36661 aagacctcct ataacaatgg ttgaaaaaaa tattactata attcttatcg gattacaagg
    36721 atattgagcg atttttttaa atatttagat aaaattctgt aaaatatatt ttcaaagagt
    36781 ataaaaataa tgttagggta taagtaaata aagatatata tagggggcta tcattatgga
    36841 taaagaatct gtcgtagcaa gtttagcaag aaacaaaaag attgctgtag aaacaatgac
    36901 cggtcaaagg tacatcatag aacgcatttt acatacaaat gatgaaaaac atattcatat
    36961 tttgaaacct aaagatgtcg tgttagatgt agataccatc aaagacatcg acgaaaatca
    37021 tttagatgat gcaacataag tataaaacga aacatgcgcc tatgacattg attaatgttt
    37081 taggcgctac aatgttttat ttatgttagt ttgtcgaagt atggctcatc taatggattc
    37141 aattgcaagt tttcaatttg caacgttgaa tgtgcaatgc catatttttc tttcaacaat
    37201 gatgatactt gatcaatcgc ttgataatca tccccctcat attttttatc taacacaaca
    37261 tgggcactta atgaataatg ttctgttgta atactccaca aatgaaattc atgtacatct
    37321 aatatgccat ctatgttttt tatatctgct ataatttgat cagtatccaa atgttgaggc
    37381 acactttcca ttaaaattaa ccacgcatta cgcgtaattt tataaccacc acgtaaaatg
    37441 atgagtgaaa ttacaatact aatgattgag tcaatgatgc gccatcctgt aaagtagatc
    37501 aatacaactg caacgatgac accaatagag ttcaataagt ctcccatgaa atgccataat
    37561 gcactttgaa tattgatatt gtcttcttgt tttaaagacc ttacaaggat aacagtcaaa
    37621 ataatattga cgagtaaacc aatactagca atcataaaca taatgccact ttcaatgggt
    37681 tgcggataaa taatacgtac aatagcttca tataaaatcc agattgaaat tacaattaat
    37741 gctaaaccat ttaaaaatgc cgctaatatc tcaaatctta aatatccgaa tgtgtagcgt
    37801 gcagtcggct tcttactagc aaaataaatt gccaacattg acaaaccgag tgctaataca
    37861 tcactaagca tatgaaatga gtctgacagc aatgctaatg aattagatac taaacctcca
    37921 acaaattcaa tcactgtaaa taatagtgtg atgataagtg atgcccatag cgtcttttta
    37981 gaactttgtt gaattttttg atgatcgaca tgatgaaaat atttgtatct gtttatcgta
    38041 ttcgcctttt tcccgttcat agtttgccac tcctaaataa atgcgtcgta tttaatgtag
    38101 ttgcttttaa taacaattat ttatagcttc tcaggttagt attaatgctt ccgtaattaa
    38161 ccataattta tacatcatcg tactttacat gacaactcat taatctttac cctttatacc
    38221 tttatgtaat atcaccttgg aaaaactttt acagcacact cgcgctttgc tttatcccta
    38281 tcatatagaa atttttaaac atttccgata tgattgtgct aattacataa aaatgacaaa
    38341 aaagtttctc aaattcattt gttttatttc atcaagtcca aaaataacaa tagtatttta
    38401 tgattgttcc acatattaaa acaaacgata catttgcata tagaacttca tatttcaaaa
    38461 tcaccttaat tcataaacaa ctctgtcctt ataatagccg catcgcaaat tagcataagt
    38521 aaaaacagca gtaagatgat ttgcaattaa aaaccatcat actgctatta ttttagtcat
    38581 tatatcctag tatcattatg caagttccta aatattttgt ttcgcttaaa atctaaacaa
    38641 ctccgtaaac atgctaagac ttaccgctag actgcctaca aacacgcttc aaaaatgata
    38701 tttattctag tacaaacctt ttaaagcatt gcttgtatca atataaatat ttttaacttg
    38761 ttgatagtta cttaacgcac ctttataagt ttctcgaccg atacctgatt ttttataacc
    38821 accaaatggt gcgccttctg gtacttggtt gtaagtgtta atccaaatac gtcctgtacg
    38881 aacagcttta gcaatattta atgcacgtgt gatattttga gaaaatacgc cgccagctaa
    38941 accatactct gaatcattag ctatatcaat tgcttcttga tcatccttca ctttaatcac
    39001 tgttaaaact ggtccaaata tttcttcttg cgctaattta tgatgattat ctggcacagc
    39061 aattaacgtt ggttcaaaga agaatccttt atctaaccca ttttcagtta aacgatgacc
    39121 acctgctaaa atttgtgcat ctgattcttt tgctgcatca atatatgatt gaattttatc
    39181 taattgatcc ttaccagttt gactacccat ttgtgtagct tcatcttgtg gatctccaac
    39241 tttaatattt gaaaatgcct cttgtaaacg tggcaccaat tgatcataaa ttttttcatg
    39301 aactaataat cgagaacctg cactacatac ttcaccttgg ttgaataaaa tacctaactg
    39361 aataccttca actgcaaggt ctaaattagc atcatctaat atgatattgg cgcttttacc
    39421 accaagctct aaagtagcag gtactagatg tttcgctgca gcttcggcaa cttgataacc
    39481 tacatcagtt gagcccgtaa atgataattt atctacccca tcatgattga aaattgcatt
    39541 acctgattct gaacctttac ccgttagtat attgacaaca ccattaggta atacctcttg
    39601 gaaaatttta gcaacttcca ataaacttaa tggtgttgaa gacgaaggtt gaatcacaat
    39661 tgtattacct gcagcaatgg ctggcgcaat cttccatgca gctaatagca ttgggaagtt
    39721 ccaagcaaca acagcaccta cgacgccaat cggctcatgt cgtacgatac tcattgtgtc
    39781 tttatcgata tcattcactg taccttcttc tgtttcaata acacttgcga aataatggaa
    39841 atgtcttgca gcaaatggaa tatcaattgc tgttgtctca cgaatcggtt taccattatt
    39901 taatgtttca atcattgcaa ttttatcttt ttgtgccatt aatttatcac caatatcacg
    39961 caacatttgt gcacgttctg atttagaagt taatgaccat gattcaaatg cctcttgcgc
    40021 cactttgact gcatgatcga catctttatc ttttgctctt gtaatatgtg atagtgtttc
    40081 tccagttgct ggattagtca cttcgattgt ctcgtcactg ctacctttaa caaattcccc
    40141 attgataaat aaaccataat tctctgctat ataatctcga acgtttactg ccatattatc
    40201 tctcctttgt ttattggcta taatataaat ctcttagcat catatttaat aatgctttta
    40261 aacgatatga ttcacattat atagtaagcg tttacatttt ataaaataat cagctcacag
    40321 aagtctcatt tttaaaactt ttgcgtcgtt tttgcacata aaaataatct tattttcgaa
    40381 aacatattgc acttttcaaa aataaaacac ataatataac tataatactt gagaataatt
    40441 ctcattaaat agtttggttg ttatgaaagt cgcgcttaaa atgcgttaat gggacaagga
    40501 taattcaaca acgtttcatc ttactgagtc attgcactta tcatacacat tatatttagc
    40561 atgagttata ttactaaaac aatattacat gcgcattagt actagaatgg agttttttca
    40621 atgcgactta ttttaatcgt cattggctta atatttacag cgcttggtat tgcaggtgcc
    40681 gtattacctt tactgccaac gacccctttt ttactcgtag cagttttttg ttttgctcga
    40741 agttcagatc gcttttacaa ttggcttatt aatcaaaaaa tttataaaga atatgtagaa
    40801 aacttttatt tacatcgagg ctacacgcta caacagaaaa ttaaaatttt aattagctta
    40861 tacattgtaa taggtttttc aatttatatg gtggatgttc ttgcagtccg tataggatta
    40921 atcataatgg ttatcataca aaccgctgta ctctttacat ttgtaaaaac attacccaaa
    40981 tcaaataata aaatagagga gtgattgccc atgtttatgg cagaaaatag attacaatta
    41041 caaaaaggca gtgcggaaga aacgattgaa cgtttttaca atagacaagg tattgaaact
    41101 attgaaggct tccaacaaat gtttgtcact aaaacattaa ataccaagga tacagatgaa
    41161 gttaaaatct taactatttg ggaatctgaa gatagcttta ataattggtt gaattccgat
    41221 gtatttaaag aagctcataa aaatgtacgt ttaaaaagtg atgacgatgg acagcaaagt
    41281 ccaatattat caaataaagt attcaaatat gatattggct accactatca aaaataaaca
    41341 tattaaaaac ccctagtcac cgctggttgt ctaaaccaac ttaatgatta ggggtttttt
    41401 attatttgcg acgtaaaggt acgaattcat ccggcttgtc tgaacgcaat ccaaaataat
    41461 attctattgc ttcacaaata cgtcgtgatg cttgtccatc accatatgga ttattcgctt
    41521 gactcatacg ttgatacgct tcatcatcct caatcaattg tttcacattt cgaacaatat
    41581 tgtcataatc tgtgccaatt actctcgatg ttcccgcctc aacgccttcg ggacgctctg
    41641 tatgattcct taataccaac acaggttttc caaatgtagg agcctcctct tgaataccac
    41701 cagaatctgt cagcacgagg tacgattgat ttgtaaaatt atggaactca agcgcatcta
    41761 atggctcaat taattcaatc cgatttctcc cagataaata tttttcggca atcgctctta
    41821 cctttggatt acgatgcatt ggataaatga agacaacatc tttatattca tctgccaaat
    41881 ctcttactgc tttaaaaatc tgatgcatcg gttctccaat attttcacga cgatgcgctg
    41941 ttagtaaaat aactttcttg cctttatgtt tattaataat cgttgaaaca aactcatttt
    42001 gaactgttgt tgacaatgcg tcaataactg tatttccggt aataaagata ctctctttgt
    42061 ctttgttttc aaaaagtaaa tttttagctg caattactgt tggcgcaaaa ttcaattcag
    42121 caatattact taccatgact cgatttaact cttcaggaaa tggtgagtat ttctgatgtg
    42181 ttcgaagtcc agcttctaca tgtccgaccg gaatttgatg ataaaatgct gccaagcttc
    42241 ctacaaaagt cgttgtagta tcaccatgta ctaaaatcat atccggctgt tccccattaa
    42301 tgatgctatc aagtttagca agcgcattcg ccgtaatgcc cgctaacgtt tgttgatctt
    42361 gcataatatt taaatcatga tcagcttgaa tatcaaatat acttaacaca ctatctaaca
    42421 tatctctatg ttgtgctgta atcacaatgt tcgcttcaaa gttcccatta tgatcaattt
    42481 cttttactaa tggtgccatt tttattgctt cgggtctcgt accgaaaata accataattt
    42541 ttttcataac attatcctct ctgaagttca aacacatgac agcttatctt tccttaatca
    42601 tgtatgccct agtttgacac attttatttg tcgataaaat taaatatatt gccataatta
    42661 taatacgata catcttcaaa tgatgatttc acaacatttt ttgtatcaaa aatcacttta
    42721 tgcttcattt tatcaaaatg actgtccgat aaatttttaa attctgagtg gtcacttaaa
    42781 atcaatacta gcgatgcgtc tttgacagca tgtgacatat catgttccac aaaatctaat
    42841 tcaacatgtg gatcataagc acatacttct atgtctggtt cttgatttaa tagctcataa
    42901 atatcaaatg ctggtgattc tcttatatca tcaacatcac ctttataagt taaaccaaat
    42961 actgtgactt tattcccgct caacgctttg atgatttgct tcgttgtatc aacaacatag
    43021 gacggcattg aattattaat ttcacgtcca gtttgaatta actttgcatt ttcagggtct
    43081 ttagcaataa taaagtacgg atcaacagct aaacaatgac cgcctacacc tggaccaggt
    43141 tgatggatat taacacgcgg atgtttgttt gccatttcaa tcacatctaa tacattaata
    43201 tttaagttgt tgcaaatttt tgttaattca ttagctaaag caatattcac gtctctatat
    43261 gtgttttcca ttagcttact catttcagca gtacgtgcat ctgtttcaat catttctccc
    43321 tgaacgaatg tgcgatagac acgtttcccc gcttcaatac aagctttagt cacaccgcca
    43381 atgatgcgat tgttatgaac taattcttct aaaatttttc ctggcagtac acgttctgga
    43441 caatgcacta aataaatatc ttcacctatt gtaaacccta aattttcaat gactggtttt
    43501 acaaaatcat ccatcgtttt aggcgcaatt gtcgactcta caataatggt atttcctttt
    43561 tctaaaaatg gtaaaatact atctaatgca cgcataacta gcgaaatgtc acatgaccgg
    43621 tactgatcat cattattcgg tgtcggaacg gcaatgataa aaacatcaga tgcttctggc
    43681 gttgtagata ccttcaattt tcccgatgac agtacctctt cataaacctc ttgtaatcca
    43741 ggttcttcaa tactaatttg accactttgt aacttatcaa tcgtttgctg attaatatca
    43801 acaccaagca catcgacgcc atgctttgca aacataattg atgttggtaa accaatataa
    43861 cctaagccaa ctactgttaa cttcatacta taacctccaa ataaaacgat ttgtatgttg
    43921 attttattta atagatgact tatgccttat ctttggttgt cgtttcatcc atgatgtccg
    43981 caataacaag tgacattttt ccaggaataa cttcaagcgc attattattt tcatataaag
    44041 tattgctgta tgttaaattg ccgaataatt ttctaaagac cgacaactta ttaaaatact
    44101 tatttagcat tgaaggcatg ttgatcaata gcgttttacg atgcgattgg cgacgtattt
    44161 catacattac tgacgatgta tcaaagtaaa aactatcttg aggatgatac acgcctgtca
    44221 cttctaatga tattaattga tcaataaatg ctgtcagatg tttaatatat aatgcactcc
    44281 gctgattatt aatatttgga atgattggca atcgctttga caattgcatt aaccgttgga
    44341 aatttcctgg gcaatgtgca ccataaatca ttggtggtct cacaattgct actttaaacg
    44401 aatcactaat caactcttgt aatgcttgtt cagcgaactt tttggaaata ccatagttgg
    44461 tcgtagggtt cattggtgtt tgtgtatcaa tttgatctga tttaccaacc tgaccttctt
    44521 ttccataaac tgccatagta ctcataaaaa taaattgttt aacgtcttca gctttagcct
    44581 tttgtgccaa ttgtttcgta agcaacatat tcacttgcat ataatcagat agccttgctt
    44641 gaggtgaatt gttgtgaacc aaagctgctg tatgaattaa aacatcataa cctttgaacg
    44701 aggtcgactt ccataattga ttcctaacat taatttgatc cacttgatgt ccttgttcaa
    44761 taagcttatc ttttaaagca ttaccgatat atccatgtac gcctgtaatt aaaatatttt
    44821 ttctcattag tgatgcacac cttctgaagt aacgatattt ttaattgttt tatatatgat
    44881 atacatatca agcatcatag attgatgtgt taagtaataa tgatcatacg ctactttttg
    44941 atcatcagta atatcatctc tccccatcac ttgagctagc cctgttacac ctggtctaat
    45001 cgtatgcacg ttcgcttttg tacgtttttc aattaattcg tattgattat aaagcgctgg
    45061 tctaggacct acaattgaca tttctccttt taaaacattc aataattgtg gcaattcatc
    45121 aatagaggtt ttacgaatga ccttccctgt ctttgttata tacgctgttg aatccattaa
    45181 atcagttgca acattaggtg tgtctatttt cattgatcta aacttataaa tattaaacaa
    45241 ttcattatta atcgtcggtc ttttttgttt gaaaatggct ggtccgggtg attccatttt
    45301 aattagtaat gctgtaatta acagaatcgg acttaaaact actaaaccat atattgaact
    45361 cactacatcg aataatcgct tcataattat ctcctcactt caacagattg taatacttca
    45421 tagtattaat ttctctcgta tatttatctt taaacagttg atgtgcgcgc tcacccatct
    45481 cgtgcaattc cttactcgag tgagtgttga tgaagttata aatggcatgt gcatcgccat
    45541 tatcaatttg gatacccgca tcatattgtt caacatgttg aacgatatca gattgcttat
    45601 ccatgatgag taccaacggc ttcttagctg caaggtagcc ataattcttg ctcggcacgc
    45661 ctaaaccgac gccttcttta atcagcgatg caatacatac atccgcaatt tttaatacgt
    45721 cagcatagtc tgtacctgtt aaaaactcaa acattttaac attttcaata cgatggtctt
    45781 ctattgccgt tttgacatct gcaaatttct taccatgacc acaaagtatt gttaacgttt
    45841 gaggctgatc cttatttaat tttaaaaatg agataagtgt ctccatatcc tgtaactgcc
    45901 ccatattacc gctatacaat aaaattttgt cgtattgctc acggtaagct ttaaatgtgt
    45961 cattatagat acgattgtct tgtaactgac gcatgtcata ccagttagga atcacatgaa
    46021 tattgtcagg atttttagaa atttgatgat ttagtaagta gtttttcatt tccgtaccaa
    46081 ggacaatgac attttcagca ttcttgtaga catgtttatt aatgtaacgc atcagtttat
    46141 caatcatgct acctggacgt gttgcacctg tcttaatcgc attatcaggt gctatatcat
    46201 acaccacaaa agaatatttt ttcttaagca atctgtgtag aacgtctggt attaatggca
    46261 agattggtgg attagagtaa acaagaatct gatcatattt caacatttta ggtatattaa
    46321 tcacgaattt tgaaaataaa ctaaagaaat tgatgatcct tccaacctta cttttgttat
    46381 taaacctcga atacttgaga cgtcgaatgc gaataccacg atgcatctcg gttttagaaa
    46441 cctgtttatg attactatat tcatatggcc atccacacat gacatcgaca ttaatgtgat
    46501 tcgcaattaa atcttccgcc aattgtgttg gtaacgtcgc tgaagataca tattccggat
    46561 aaaaatactg acataaaatc aaaatctttt tttcactcat ctaatcgacg tcctttttat
    46621 taatgaattt agactaagaa tttttgaact gtttattttt agcaaaataa tagagtgcta
    46681 tgaaaaatat catgtacgat acgattaaaa caactgtatt aatagataca aagtttaaaa
    46741 aagttaaatt aagcattttt gatataacaa atgttgcagt agtacctatt aaaaataacg
    46801 cttgcagtat taattctaac tgttgttttt gtaatacaat cacagaaagt gataccgttg
    46861 atacaatcag ccttataaca aataatggaa taacaatttt tataactgta attgcgtcaa
    46921 cccagctatg tcctaataat atattgacta attcttcact gaacaaataa agtggcacat
    46981 aaagaattat agaaactata accagtatgg aactaaattt aagtaataaa tttcggaagt
    47041 taccatattc aatataatag tcattagctt gttgcataaa tatttttgat aaccctaatg
    47101 aaataattgt cactggtatg cctagtactc ttatggccat accataaata cctacttctg
    47161 tattggtata caaaatacct ataaagaaca caacaaccga gaaagacaaa ctatttaata
    47221 atattgatgg tgttgaataa actaactgat ttttattttc taaaaataat gctttagttt
    47281 cttctttatc tctcacaata ttaaagtgct ttttaaacgt tttatatcct agtgtaatgc
    47341 ctgcgatata ggataatgaa aaaccaataa ttaatcctag acttcctaat gctaaaagtc
    47401 cgaaaataat ttgaagtaaa gccattgatc cagctctaaa cacattaatc aaacttaaca
    47461 ctttatattt tctttcctta tttagataat ttgtaaaaat gtttgttaaa cttaccaaaa
    47521 acaacataat aataatgaaa ataaaatcta atataaagtt tgggtataac catgcactaa
    47581 tcgcaaatat tggtatcagt attaataaaa taagcaatga tatcaaaaaa ctgatttgtg
    47641 aaagtatatt agcactacgg tcatttttag tattcacaat caacaaatcg tatcttgcat
    47701 ttattattgg tattaaaatc attgcgatat ttgaaaaaat tgtaaactca ccaaattctg
    47761 taggtgaata tagtcttgta ataattgggg tagtgatgat taatatgact tgagcgatgc
    47821 cactgcttaa aatcattaag aacgaatcgc caataaattt atttaatctc ataattaacc
    47881 tcagattttc ttgatgactt tcgctggatt gccacctata acaacgttac cttctggaaa
    47941 actttttgtt acaaccgacc cagcccctac aattgtatgt tcccccaatt ctactcctgg
    48001 taatataact gagttcattc caatccaact ataattccct attttgacat cttcacctgg
    48061 tgcatgtgac ttcaaatttt taatattatg attagctgta ataataccta cgtttggcgc
    48121 tatataaaca cctctaccta tatatataac tgctgaaaaa ttattaaaat acgtcccggg
    48181 cgattggaaa atatgaatat catttttatc aaaaacaatg ttattagggt tatgcattct
    48241 aacagttata tctgcaggaa atggcaagct cgtatttatt cctaacaact tgaacggaac
    48301 agctttccat gcccataacc atccagccac tttattttca aaataatatc cagttaaata
    48361 ttctttctta taaaataata atagtggtgt attcattaat ctaaatattt tcgatttcat
    48421 aaatgttttt acaatcattc tatccctact tttttagtaa atttttttat tataatgcct
    48481 gggataatag ctataacata tacaactaaa atcatagaaa tcaatgtgat taatggtgtc
    48541 gggtgactaa aaatactcgt aataagtctc atataaatat atgtaccaat aaatatcact
    48601 tctaacgaat gactcttccg aataatatac tcactcagaa atgcaaaaaa gatatttgca
    48661 attaaattca aatagaagaa taagggtcca aaataaataa ttccatatcc agcactcgaa
    48721 attagatgac cagtcaattg tttactacca tatatcaatt ggttaaaaag ttggctagta
    48781 atcaactgct ttttatctac aaagaaatta aacccaaaaa cagctctagt attatcaaat
    48841 aaatattgtt taaacgaacc attatagtaa ttcaaatagt ctatagaagc tgcaatattg
    48901 ctatgtccat aaaaatatga ctgcaacgtg tcaactatct taagattact tacactcgta
    48961 ctatttaacg cctcgctata tgaactataa ttaaacacat acaattcttt atatagtgtc
    49021 atcaatgcta atacgaaaat accaacagat ataataatga tattgatacg tcttttgtgc
    49081 ttagaaaaca agagtgacac cactgttaaa acagcaacca tcgtataaag ttgataactt
    49141 cttctttcac caacaattaa actaatattt aaaatcccta tagctagcgg caatataaca
    49201 taaataattt taggattctc tttatacttt ttatatgaag catatgcgat tattaagaat
    49261 aataacgcta aggcaagttg aaatagcatt acaaaaagga catttaaacc acttgttgct
    49321 tcggttcctc ttcccattgc atctgtttta ataattaaaa atgatatgtt tttgcgcact
    49381 tcgggaaaag ctacaaaaat cccacaaata acaagaccaa acacaatgta ataaattgat
    49441 gatccagaaa gtgtaaagct atttcttgat accacttcat gtttgaataa tttagatagt
    49501 atcaatataa atagcgagcc tatgataaac tccacactca tataaataac cgcttgatta
    49561 aaataatctt gtcctaatgg taaattatct atagttgaat cctggaaaaa cactatcata
    49621 gcaggtataa atactaatct aagaaaacct aaaacatcgt atacaatttt cgaaaattta
    49681 aaattcggat tatttgagaa gcttataaag agctttccta ctccataact tataggtgct
    49741 aaaaacaata aaggtttatc acctaacata aggcaaggaa acattaaaat ggcaataaca
    49801 tatgaagtga cattatatat cttattcatt aaatatcctg cctatctctt gatagaactg
    49861 tttattattt tcaattacat cgtttctata agcttctctt ttatgattaa tgtccattac
    49921 attcaaattg ttataccaat ccatcaattc atcaccaata ccttttaaat tttctggatt
    49981 aacactaaaa cccagtccga atttattagc ttctgtagcg gtgaatgtat catctgttgt
    50041 aataataggt ttgtttaaaa acaacgcata atacattctt atgga
//
LOCUS       DATTQF010000005        49660 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.005, whole genome shotgun sequence.
ACCESSION   DATTQF010000005 DATTQF010000000
VERSION     DATTQF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 49660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.005"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            23..4078
                     /gene="sasA"
                     /locus_tag="VB241_000214"
     CDS             23..4078
                     /gene="sasA"
                     /locus_tag="VB241_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HEP1243549.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKITGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTTDSTSVTKNSSSEQTSNSDTVSSEKSENVTSTTNSTSNQ
                     QEKLTSTSESTSPKNTTSSSDTKSVTSISSTDQQTNTSTNQSTASNTTSQSTTPASAN
                     LNKTSTTSTSTAPIKLRTFSRLAMSTFASAATTSAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNAAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSAADNAAKLKVQPTNNTFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSVSMSTSGSLSKSQSLSTSTSDSASTSQSVSDS
                     TSNSISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSDSVSGSLSIATSQSVSTSSSDSMSTSEMISDSMSTSGSLAASDSKSMS
                     VSSSMSTSQSGSTSESLSDSISTSDSDSKSLSLSTSQSGSTSTSTSTSSSVRTSESQS
                     TSGSMSASQFDSTSISTSFSDSTSDSKSASTASSESISQSVSTSTSGSTSGSTSTSTS
                     ESLSMSGSDSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSMSLSTSDS
                     TSTSESLSTSMSGSQSISDSTSTSMSNSTSMSNSTSTSMSGSTSTSESNSMHPSDSMS
                     MHHTHSTSTSRSSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNG
                     LLGGVMTLLVGLGLMKRKKKKDENDQDDSQA"
     gene            4207..5418
                     /gene="secY2"
                     /locus_tag="VB241_000215"
     CDS             4207..5418
                     /gene="secY2"
                     /locus_tag="VB241_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEP1243550.1"
                     /translation="MLKLLQQYEYKIIYKRILYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLITLVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMRSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKDVSDDMPMMTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            5429..6982
                     /gene="asp1"
                     /locus_tag="VB241_000216"
                     /pseudo
     CDS             5429..6982
                     /gene="asp1"
                     /locus_tag="VB241_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
     gene            6972..8540
                     /gene="asp2"
                     /locus_tag="VB241_000217"
     CDS             6972..8540
                     /gene="asp2"
                     /locus_tag="VB241_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEP1243551.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIEETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            8518..9477
                     /gene="asp3"
                     /locus_tag="VB241_000218"
     CDS             8518..9477
                     /gene="asp3"
                     /locus_tag="VB241_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEP1243552.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDNLEYKSMRVTKIG
                     DDQYGNDRINVIIAEPSDAYPTISNDFLKSFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWFTEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            9467..11857
                     /gene="secA2"
                     /locus_tag="VB241_000219"
     CDS             9467..11857
                     /gene="secA2"
                     /locus_tag="VB241_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEP1243553.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDVLKQKTL
                     EFKERIASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVFFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKVLAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQIALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAN
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            11875..13383
                     /gene="gtfA"
                     /locus_tag="VB241_000220"
     CDS             11875..13383
                     /gene="gtfA"
                     /locus_tag="VB241_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEP1243554.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFEDQEVIWLYQYFSDISIAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNENGSITYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYTD
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVVAKLAHAIVMYFNNGPQTPHYISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            13376..14734
                     /gene="gtfB"
                     /locus_tag="VB241_000221"
     CDS             13376..14734
                     /gene="gtfB"
                     /locus_tag="VB241_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEP1243555.1"
                     /translation="MIKLFDYFNGHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVL
                     VWQEQCDGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVKSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAF
                     ES"
     gene            14976..16859
                     /gene="sasF"
                     /locus_tag="VB241_000222"
     CDS             14976..16859
                     /gene="sasF"
                     /locus_tag="VB241_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HEP1243556.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAVSEKDTE
                     ISKELLSKQDLLDKVDKANRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKGSQIEMNGRSDIDSKLDQLLKDLNEVSSKVDRGQQSDEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLSQQIHKSEDSAVSTTDNNHEVAKTPNNDGSG
                     HVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTYHKLNTLQS
                     LDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKHATQRILESIF
                     NKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQ
                     ILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDK
                     KQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQ
                     TKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPDIPTPKPEK
                     TLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLK
                     NKA"
     gene            17027..17587
                     /locus_tag="VB241_000223"
     CDS             17027..17587
                     /locus_tag="VB241_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP1243557.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(17852..19711)
                     /locus_tag="VB241_000224"
     CDS             complement(17852..19711)
                     /locus_tag="VB241_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HEP1243558.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSTDDETSKDTTSKDIDKADNNNTSNQDNNDKKVKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKVNQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDNMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            19922..22903
                     /locus_tag="VB241_000225"
     CDS             19922..22903
                     /locus_tag="VB241_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP1243559.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQEAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVTSTPPSAPNGNTTPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKTANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     SILKNGKTRIDRLQTVLPSIEQQYISAIKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFAQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLEPVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(23015..23953)
                     /gene="manA"
                     /locus_tag="VB241_000226"
     CDS             complement(23015..23953)
                     /gene="manA"
                     /locus_tag="VB241_000226"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP1243560.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLTDMIDNHEFDSLFKRIPVKSGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLAV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(23967..25919)
                     /locus_tag="VB241_000227"
     CDS             complement(23967..25919)
                     /locus_tag="VB241_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HEP1243561.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANMIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHILWFIFAVIVGSL
                     VTMITVLLLKRRTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTIIIADHNMSRNEAIDTLIHQLKVCRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(25996..27869)
                     /locus_tag="VB241_000228"
                     /pseudo
     CDS             complement(25996..27869)
                     /locus_tag="VB241_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            complement(28109..28582)
                     /locus_tag="VB241_000229"
     CDS             complement(28109..28582)
                     /locus_tag="VB241_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243562.1"
                     /translation="MKKFLITVVIILLIGALGFAIYAAINHSTSSNHNNEQDNKANHQ
                     KKDTEVNHQKDENTNNTNENQNSNNGVTNGNQSSTPSTVQNNGSTAPANTQPSNPNQS
                     NSNQSNSNQGGSSQPSTNNQNHKQQNTPAPNKSNNANTNESSKQASPSQPQSSKN"
     gene            28838..29365
                     /gene="isaB"
                     /locus_tag="VB241_000230"
     CDS             28838..29365
                     /gene="isaB"
                     /locus_tag="VB241_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HEP1243563.1"
                     /translation="MNQTTKLCAATTLALGTLIGATVVGTASPIHQEAQAATTPYYTY
                     HGYIGHNANFIMDKHFINAIKYDNVTFNGIKLAKTNATKKVEKYDQTFKGVTAKGNEA
                     NQLQFIVKNNISLKDIQKAYGKDLKKENNKTKESDSGIFYYQHSKKSLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            29779..31308
                     /gene="aur"
                     /locus_tag="VB241_000231"
     CDS             29779..31308
                     /gene="aur"
                     /locus_tag="VB241_000231"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HEP1243564.1"
                     /translation="MRKFSRYAFTSMATVTLLSSLTPAALASDTNHKPATSDINFEIT
                     QKSDAVKALKELPKSENVKNHYQDYSVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDEAADKAFNAVKIDKNKAKNLQDDVIKE
                     NKVEIDGDSNKYIYNIELITVTPEISHWKVKIDADTGAVVEKTNLVKEAAATGTGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAYNFNDQTGQATLITNEDENFVKDDQRAGV
                     DANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            31675..32124
                     /locus_tag="VB241_000232"
     CDS             31675..32124
                     /locus_tag="VB241_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP1243565.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFKHYCEHEVLSSIINGAYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            32127..32294
                     /locus_tag="VB241_000233"
     CDS             32127..32294
                     /locus_tag="VB241_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243566.1"
                     /translation="MYQMNNKNLFCITCTQVSYYILTCVRVFLWKERIHSHSFDCIKN
                     FIQHVFVGVFE"
     gene            32471..33706
                     /gene="arcA"
                     /locus_tag="VB241_000234"
     CDS             32471..33706
                     /gene="arcA"
                     /locus_tag="VB241_000234"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP1243567.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKEVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTIHSAILKAEGNMNIFIIEYDDVNKDISIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            33739..34749
                     /gene="argF"
                     /locus_tag="VB241_000235"
     CDS             33739..34749
                     /gene="argF"
                     /locus_tag="VB241_000235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP1243568.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKDAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQLFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            34840..36270
                     /gene="arcD"
                     /locus_tag="VB241_000236"
     CDS             34840..36270
                     /gene="arcD"
                     /locus_tag="VB241_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEP1243569.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLASIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            36287..37228
                     /gene="arcC"
                     /locus_tag="VB241_000237"
     CDS             36287..37228
                     /gene="arcC"
                     /locus_tag="VB241_000237"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP1243570.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGGLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPGSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            37327..38031
                     /locus_tag="VB241_000238"
     CDS             37327..38031
                     /locus_tag="VB241_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP1243571.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            38381..41002
                     /gene="clfB"
                     /locus_tag="VB241_000239"
     CDS             38381..41002
                     /gene="clfB"
                     /locus_tag="VB241_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HEP1243572.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNTIETPQLNTTANDTSDISANTNSANVDSTAKTMST
                     QTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASDFKLEKTAFDPNQSGNTFMAANFKVTGQVKSGDYFTAKLPDSVTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFDNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKKRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTGEFKDKISYKYDNVASINFGDINKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPVDPTPGPPVDPEPEPEPTPDPEP
                     SPEPEPEPTPDPEPSPEPDPDSDSDSDSGSDSDSGSDSDSDSDSDSDSDSDSNSDSES
                     DSDSDSDSDSDSDSDSDSESDSDSDSDSESDSDSDSDSNSDSDSDSDSDSDSDSDSDS
                     DSNSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSESDSESDSDSDSESDSDSDPDSESDSDSDSDSDSDSESDSESDSDSDSDSDSDS
                     DSRVTPPNNEQKAPSNPKGEVNHSNKASKQHQTDALPETGDKSENTNATLFDAMVALL
                     GSLLLFRKRKQDHKEKA"
     gene            41118..41879
                     /locus_tag="VB241_000240"
     CDS             41118..41879
                     /locus_tag="VB241_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP1243573.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            42124..42579
                     /locus_tag="VB241_000241"
     CDS             42124..42579
                     /locus_tag="VB241_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1243574.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(42682..42792)
                     /locus_tag="VB241_000242"
     CDS             complement(42682..42792)
                     /locus_tag="VB241_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243575.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(42862..44286)
                     /locus_tag="VB241_000243"
     CDS             complement(42862..44286)
                     /locus_tag="VB241_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEP1243576.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            44484..44684
                     /locus_tag="VB241_000244"
     CDS             44484..44684
                     /locus_tag="VB241_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243577.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            44710..45375
                     /gene="nsaR"
                     /locus_tag="VB241_000245"
     CDS             44710..45375
                     /gene="nsaR"
                     /locus_tag="VB241_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HEP1243578.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQSIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            45386..46273
                     /gene="nsaS"
                     /locus_tag="VB241_000246"
     CDS             45386..46273
                     /gene="nsaS"
                     /locus_tag="VB241_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HEP1243579.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQFNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            46381..47136
                     /locus_tag="VB241_000247"
     CDS             46381..47136
                     /locus_tag="VB241_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243580.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMKLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLVFQQRITDSLALVNGGSVNI"
     gene            47133..49133
                     /locus_tag="VB241_000248"
     CDS             47133..49133
                     /locus_tag="VB241_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243581.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFMIISVISIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDTVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTIMKLITNDYDLSFNDNSKSQ
                     IERQQTIEHIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGSIFSKQSVMITALTVQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKKSTISINFNVSGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRISKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMLG
                     INNHHIFLTCYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT        17527 a         7503 c         9161 g        15469 t
ORIGIN      
        1 agataataca aggagttatt acatgagtaa aagacagaaa gaatttcatg acagcttagc
       61 aaatgaaaag acaagagtaa gactttataa atctggaaaa aattgggtaa aatccggaat
      121 taaagaaatt gaaatgttca aaattatggg actaccattt attagtcata gtttagtgag
      181 tcaagataat caaagcatta gtaaaaaaat aacgggatac ggactgaaaa ctacagcggt
      241 tattggtggt gcattcacag taaacatgtt gcatgaccag caagcttttg cggcttctga
      301 tgcaccttta acatctgaat taaacacgca aagtgaaacc gtaggcaatc aaaactcaac
      361 gacaatcgaa gcatcaacat caacaaccga ttctactagc gtaacgaaaa atagcagttc
      421 ggaacaaaca tcaaatagtg acacagtctc aagtgaaaag tctgaaaatg tcacttcgac
      481 aactaatagt acaagcaatc aacaagaaaa attgacatct acatctgaat caacatcccc
      541 aaagaatact acatcaagtt ctgatactaa atctgtaaca tcaatttcaa gtacagacca
      601 acaaactaat acatcaacaa atcaaagtac tgcatcaaat actacttcac aaagcacaac
      661 gccagcttca gccaatttaa acaaaactag tacaacgtca accagcacag cacctatcaa
      721 acttcgaact ttcagtcgat tagctatgtc aacttttgca tcagctgcaa cgacaagtgc
      781 agtaaccgct aatacgatta cggttaataa agataacctc aaacaatata tgacaacgtc
      841 aggtaatgct acttatgatc aaagtaccgg tattgtgacg ttaacgcagg atgcatacag
      901 ccaaaaaggt gctattacat taggaacacg tattgactct aataaaagtt ttcatttttc
      961 tggaaaagta aatttaggta ataaatacga aggtaatgga aatggtggag atggtatcgg
     1021 ctttgccttt tcacctggtg tattgggaga aacaggatta aatggtgcgg cagtaggtat
     1081 cggtggtttg agtaatgcgt ttggcttcaa attggatacg taccacaata catctaaacc
     1141 gaatgcagca gcaaaagcga atgcagaccc atctaatgta gctggtggtg gtgcgtttgg
     1201 tgcctttgtt acaacagata gttatggcgt tgcgacaacg tacacatcaa gttcagcagc
     1261 tgataatgct gcaaagttaa aagttcaacc tacgaataac acgttccaag attttgatat
     1321 taattataat ggtgatacaa aagttatgac tgtaacgtat gcaggtcaaa catggacacg
     1381 taatatttca gattggattg caaaaagtgg tacgaccaac ttttcgttat caatgacagc
     1441 ctcaacaggt ggcgcgacaa atttacaaca agtacaattt ggaacattcg aatatacaga
     1501 atctgctgtt acacaagtga gatacgttga tgtaacaaca ggtaaagata ttattccccc
     1561 aaaaacatat tcaggaaatg ttgatcaagt cgtgacaatc gataatcagc aatctgcatt
     1621 gactgctaaa ggatataact acacatctgt cgatagttca tatgcgtcaa cttataatga
     1681 tacaaataaa actgtaaaaa tgacgaatgc tggacaatca gtgacatatt attttactga
     1741 tgtaaaagca ccaactgtaa ctgtaggcaa tcaaaccatt gaagtgggta aaacaatgaa
     1801 tcctattgtg ttgactacaa cggataatgg tactgggact gtgacaaata cagttacagg
     1861 attaccaagc ggattaagtt atgatagtgc aacgaattca atcattggaa caccaacaaa
     1921 aattggtcaa tcaacagtga cagtagtatc tactgaccaa gcaaataaca aatcgacgac
     1981 aacttttaca ataaatgttg tggatacgac agcaccaaca gtgacaccaa taggagatca
     2041 atcatcagaa gtgtattcac caatatcccc gattaaaatt gctacgcaag ataatagtgg
     2101 gaatgcagta acaaatacag tcacaggatt gccgtctgga ttaacattcg atagcacaaa
     2161 taatactatt agtggtacac caacaaacat tggtacaagt actataacaa tcgtttctac
     2221 agatgcgagc ggtaacaaaa cgacgacaac ttttaaatat gaagtaacaa gaaatagcat
     2281 gagtgattcc gtatcaacat caggaagtac acaacaatcc caaagtgtgt caacaagtaa
     2341 agctgactca caaagtgcat caacgagcac atcaggatcg attgtggtat ctacttcagc
     2401 tagtacctcg aaatcgacaa gtgtaagcct atctgattct gtgagtgcat ctaagtcatt
     2461 aagcacatct gaaagtaata gtgtatcaag ctcaacaagc acaagtttag tgaattcaca
     2521 aagtgtatca tcaagcatgt caggttcagt tagtaaatca acatcattaa gcgattctat
     2581 ttcgaactct agcagtactg aaaaatccga aagtctatca acaagcacat ctgattcatt
     2641 gcgcacatca acatcgttaa gtgattcagt aagcatgagt acatcaggaa gcttgtctaa
     2701 gtcacaaagc ttatcaacaa gtacatctga ttcggctagt acatcacaat cggtaagtga
     2761 tagtacatca aattcaatta gtacatcaga atcattgagc gagtcagcta gcacctcgga
     2821 ctctatcagt atttcaaata gtatagccaa ctctcaaagt gcgtcaacaa gcaaatcaga
     2881 ttcacaaagt acatcaatat cattaagtac aagtgattca aaatcgatga gtacatcaga
     2941 atcattgagc gattcaacga gcacaagtga ttcagtatct ggatcactaa gcatagcaac
     3001 atcacaaagt gtctcaacaa gctcatcaga ctcaatgagc acgtctgaaa tgataagcga
     3061 ttctatgagt acaagtggat cattagctgc atcagacagt aaatcaatgt ccgtaagtag
     3121 ttcgatgagc acgtctcagt caggtagtac atcagaatca ttaagtgatt cgataagtac
     3181 atcggattct gacagtaagt cattatcatt gagtacaagt caatcaggtt caacaagcac
     3241 atcaacatca acaagtagtt cagtacgtac gtcggaatca caaagtacgt ctggttcaat
     3301 gagtgcaagt caattcgatt caacaagcat atcaacgtcg tttagtgatt caacaagtga
     3361 tagcaaatca gcatcaactg catcgagtga atcaatatca caaagtgtct caacaagtac
     3421 atctggctca acaagtggtt caacatcaac aagcacatct gaatcgctaa gtatgtctgg
     3481 ttcagattca acgagcgtat ctgactcaac atcaatgagt gaatccgatt caacaagtgt
     3541 ttcaatgtca caagacaaat ccgattcaac atcaattagc gactcagaat cagtgtcaac
     3601 aagcacatca atgtcattga gcacatccga ttcgacaagc acatccgaat cgctgagtac
     3661 atctatgtct ggttcacaaa gcatttctga ctcaacatca acaagtatgt ctaactcgac
     3721 ttcaatgagc aactcaacat caacaagtat gtccggctca acaagtacat ctgaatctaa
     3781 ctcaatgcat ccgtcagact caatgagtat gcatcataca cacagcacga gcacatctcg
     3841 ctcatcaagt gaagcaacaa cgagcacgag tgaatctcag tctacattaa gtgcaacatc
     3901 tgaagtgact aaacataatg gcacgccagc acaaagtgaa aaaagattgc cagatacagg
     3961 tgactcaata aaacaaaatg gattactagg tggcgttatg acattattag ttggtttagg
     4021 tttaatgaag agaaagaaaa agaaagatga aaatgatcaa gatgattctc aagcataaat
     4081 tgaatttata gttggctaaa attaaaaaga ctggggcgtt gttcactaaa aaatctatat
     4141 cagattttaa taattagtga aataacctta gtctttttaa tgtttttgat ataaaagagg
     4201 tgcaacatgt taaaactttt acaacaatac gaatataaaa ttatatataa acgtatactt
     4261 tacacatgtt ttatcttgtt tatttatata ttaggtacaa acatttccat cgtttcctat
     4321 aacgatatgc aagtaaaaca tgagtcattt tttaaaatag ctatttctaa tatgggtgga
     4381 gacgttaata ctttaaacat tttcacttta ggattaggtc cgtggttaac atcaatgatt
     4441 atcttgatgc tgatttctta tcggaatatg gataaataca tgaaacaaac aagtcttgag
     4501 aagcattata aagagcgcat cttaacatta attttaagtg ttattcaaag ttattttgtg
     4561 attcatgaat atgtttcgaa agaacgtgtt catcaagaca atatttactt aacaatcttg
     4621 attttggtta ctggaacgat gttattagtt tggttagctg acaaaaatag tcgttacggc
     4681 atcgcaggac caatgccgat tgtaatggtt agcattatta aatcaatgat gcatcaaaaa
     4741 atggaatata tagatgcaag tcatattgtg attgcgttgt taatcactct tgtgataatc
     4801 acattgttta tcttattatt cattgaatta gtagaagtga gaataccata tatcgattta
     4861 atgaacgttt ccgcaacaaa tatgagatct tatttatctt ggaaagttaa ccctgcaggc
     4921 agtattactt taatgatgag tatttcagca tttgttttct tgaaaagtgg tattcatttt
     4981 attttatcta tgtttaataa agacgtttca gatgatatgc caatgatgac atttgatagt
     5041 ccaattggta tttcggtgta tttagtgatt caaatgttat tgggttattt tttatcgaga
     5101 tttttaatca atactaaaca aaaatccaaa gatttcttaa agagtggcaa ttatttttca
     5161 ggagttaaac ctggtaagga tacagaacgt tatttaaatt atcaagcacg acgcgtatgt
     5221 tggtttggat cggcattagt tacagtcatt attggtatac cactttattt cacattgttt
     5281 gtaccgcatt tatctactga aatttatttc tcagtacaac tgattgtatt agtttacatc
     5341 agtattaata ttgcagaaac aattcgtaca tatttatatt ttgataaata taagccattt
     5401 ttaaaccagt attggtaagg aggtaattat gaaatacttt attccagctt ggtacgatga
     5461 ccaacgatgg tggcaagaca cgactgtgcc gtattatcaa ctacaaaata agacggaatt
     5521 tgacgatatg attagtttga tggggatgca ccttgaaaat gacttagatt atcgtctgat
     5581 tgttctcaat catgcaccaa atttaagaac atttttacat cgatatgact tatatgagac
     5641 aaagtattgg tctgtgtttg atgaaattca aggattcagt caccatgcgc cacaagcgat
     5701 taattatcat cacttaaaat ggccggatga tgttgagttt gtgtacacgc cgtatttatt
     5761 aaaatgtgtg acgagtgaac agacctatac aaatatttat tttagtcaag aagggtattc
     5821 aatttggttt gaagaatttg aaagagatca gttacaacgg cgttatattt ttgatgacag
     5881 aggttattta tcagctatac gttattttga tgatcaggga gaggcttctt accaagaata
     5941 tttaacgatt aatggagatt gtgtacttca tgaagatttg aaaaatggca gagtcactgt
     6001 ttcaaaaaga tatcaacatc actatcaaca aacagaatat aacaatatgg ctcaactaat
     6061 tgaagaaaaa tttcaagcaa tgattgcaca tcaaatacat gaagatgatc atgtgattgt
     6121 ggcttcagat gctaggcaca atcgacaaat agccaatcat attccggcga aatcattaag
     6181 ttattcattt tttaaaaata gaaatgaaac tgtgtcggat gaggaatatc aatctatcgt
     6241 aaagaatgcc catttaattg ttgatagtgt gcaactagaa cgtgatttaa ttagtcatca
     6301 agagaagtat cagcgagaga atacaatgat tcgaatcacg ccatttgaaa cgagacaatc
     6361 acctaatata agtagtcaat tgatggaaac atttataggt gtatggatag acggtatgag
     6421 tgacgttgat ttgcaacaaa tgatgcaacg acttgtggat tatatcgcac aagaagataa
     6481 ttaccgttta attttattaa cgcgccatca aaatgatata ccgatgtggc ttcgtgaatg
     6541 tattacgtcg gtaaatgagg aataccaagc taaacagaat gccgatgtta atgtttcagc
     6601 attaatgaca cctgaagatc aagatgacat cattgctgtt aagacgatac atgctgaaca
     6661 tgatgttgta gaagcattgc ggacgttgcg acttgtgata gatatgtcaa aagaacctga
     6721 tttgtattta caaattagtg caattagcgc tgggattcca caaattaatg gtcaacaaac
     6781 agattaagtc tctgattatg acaatggccg tattataaat acagttgatg aattagatga
     6841 tgcgttaaat tattatttat tttatttgaa aaattggaat tatgcgtacg cctattcttt
     6901 aaaattaata gatgcatatg cttctaagaa tattattaat cagctcgatg agttaataga
     6961 aggtgaaaat gatgccacgt aaatttagag ttttgcaaat tggaggagac gatttagaac
     7021 ctattttcca acacaaaaaa ggtgtgagtt gggattactt cgatattgga ttgtttgaat
     7081 ttgatagtgg ttatgtagag gctattgaag cgattgttga agcagaagga cgctttgatt
     7141 ttatctatat tcaagcacca tactcggaga cattaacgaa tttattacaa atgataagcg
     7201 aaccatacaa tacgtatgtt gatgaatcat tttggtctgt tgaatatgaa caagacgaaa
     7261 atgtccaaaa atacgttgtt caaccattac attaccggaa cattgaagaa cgtaataata
     7321 aattagaggc agttagcttc tcaggacagt atggagataa ggtttctcct aagttagcac
     7381 ttgttcatcc gaattttaaa ggagatgtcg tttaccaagg taattcagag ctcacgttga
     7441 gtggagaatt tggaaaagaa tttaaaccta tcgcatcttg gcaaaataat ctcgtttacg
     7501 ataaagataa agtcattcaa atatggccag aatttgatat tgatggtgcg gtcgagttgc
     7561 aatatacatt tagattgatt caaactggcg ctgatggtgc attaattgaa caaatcatat
     7621 tgactgatga tatgttagac agtcctttag agatacctac gaaaccattt gatgcttata
     7681 taagtgtaac tgttaaggcg cgagggaacg ggacggtaca tttaggacct atacacaaac
     7741 gatggtctag attagacatg ggtcaatttt tactcggtgg tagtcgtttt gtagatagtc
     7801 aacgacagga atttatttat tatttccacc ctggtgatat gaaaccacca ctaaacgtat
     7861 attttagtgg ttatcgaaca gcggaaggtt tcgaaggata ttatatgatg aaacgtatga
     7921 atgcaccctt tttactaatc ggcgatcctc gtgttgaagg tggtagcttt tatatcggtt
     7981 catctgaata tgaacaaggt attatcaatg ttattgaaga gacattagaa aagctgaatt
     8041 ttaaatcaca tgaattaata ttatctggtt tatctatggg gtcatttggt gccttgtatt
     8101 atggtgctca gttaaatcct caggcaatta ttgttggtaa accacttgtt aatattggaa
     8161 cgattgctga acatatgcga ttacttagac cagaagagtt cggtacagca ttagacgtcc
     8221 tggtgtctaa tgaaggtgat acatctcaag cgtcgataca ggcattaaat caaaaatttt
     8281 ggcaaacatt tcaaaagaaa tcattgtcac aaactgtttt tgccatagca tatatgcaac
     8341 atgatgatta tgatccacat gcatttcaag agctactacc tgtattgaca gcacaccaag
     8401 caagagtgat gaatcgcagt attcctgggc gacataatga tgattcacct accattgcaa
     8461 gttggtttgt taatttttac aacattattt tggaagacaa gtttgggaga gttcaacatg
     8521 ctgaaaaaca aaacatttaa agttacttgg aattatattt cacaaactac atttatgtat
     8581 ggctcaaaag tatcattttc aaatggtgaa gtaactttta taaatccttt gatgccctct
     8641 ggattaccga ttcatgagtg gttgatgtta aaacaatttt caaaatataa aagtgcacca
     8701 agtttaccaa ttttgcgtag aggacagcat tataaattgc attttgattt tgatgcaacg
     8761 cctgctggtt cagtgtattt tatcatcatt ttttataata aaaatggcac aaaactaagt
     8821 actgagattg taaagtcaaa tagtattacg attcaatatc ctgatgaagc ctatgcttat
     8881 aaaataaaaa tgatgaatgc agcatcgacg agtcttatat ttagatgttt aacaattaca
     8941 gagatgacgc atcaagataa tttagaatat aaaagcatgc gggtgacgaa gataggcgat
     9001 gaccaatatg gaaatgatag gatcaatgtc attattgcag aaccatctga cgcctatcca
     9061 actatttcga atgatttttt gaaatcgttt ggtcatgttt ggctcgttga acgttggatg
     9121 gacgacaata tcgagggaaa tattaagcaa ctaaagaatg atttgcaaag tcaagatacc
     9181 ctaaaggcaa ttaatttgat tagttatggg tcaaagtcaa atgtttttgc aacttatgtc
     9241 gctcagcatt tggattgcaa agtgtatcgt acaagccatg aagatgatga tttaaaagaa
     9301 tggtttacag aacatgtccc aggaaatcat gaactaaaag atacaaatgt cgaggtttat
     9361 tttaaagaag agcaagacaa ccatctaaat tatatgtcac gtttaatgaa tccagtacga
     9421 tttttagatt acttagatgt atcgaaactg aatggtgggg aagtaaatga aacataaact
     9481 agatgttacg ataaacgaat taagactaaa gtcgattaga aaaatagtga aacgcatcaa
     9541 tacatggagc gatgaagtta aatcatattc tgatgatgtg ttaaaacaaa agacactaga
     9601 atttaaagaa cgtatagcat caggagttga tacactagac acattgttac ctgaagctta
     9661 tgcagtggca cgtgaagcga gctggagagt attaggtatg tatcctaaag aagttcagtt
     9721 aatcggagcg attgtattgc atgaaggtaa cattgcggag atgcaaacag gcgaaggtaa
     9781 aacattaaca gcaacgatgc ctttatattt aaatgcactt tcgggaaaag gaacatattt
     9841 aattacaacg aatgattatt tagcgaagcg tgattttgaa gagatgcaac cattatatga
     9901 atggttaggt ttaactgctt cattaggttt tgttgatatt gtggattatg agtatcaaaa
     9961 aggggaaaaa cgtaatatat atgaacatga tattatatat acaactaatg gacgtctcgg
    10021 ttttgattat ttaattgata atttggcaga tagtgcggaa gggaaatttt taccacaatt
    10081 aaattacggt attattgatg aagtggattc aatcatttta gatgctgcgc aaacaccatt
    10141 agttatttcg ggcgcaccaa gattacaatc aaatctattt catattgtga aagagtttgt
    10201 agatacattg attgaagacg tgcattttaa aatgaagaag accaaaaaag aaatatggct
    10261 gttaaatcaa ggtattgaag cggcacaatc atactttaat gttgaagatt tatatagcga
    10321 acaagcgatg gtcttagtgc gtaatattaa tctagcactg cgcgcgcaat atttgtttga
    10381 atctaatgtc gattatttcg tatataatgg tgatattgtt ttaattgacc gtattacagg
    10441 tcgtatgtta ccgggaacta agttgcaagc tggactccac caagctattg aagcgaaaga
    10501 aggtatggag gtttcaacag ataaaagtgt tatggcaaca attacctttc agaatttatt
    10561 taaacttttt gaatcatttt caggtatgac agctacagga aaattaggcg aatcagagtt
    10621 ctttgatttg tattcaaaaa tagtcgtaca agtaccaact gataaagcga ttcaacgtat
    10681 cgatgaacca gataaagtgt ttcgttcagt tgatgagaaa aacatcgcaa tgattcatga
    10741 tatagttgaa cttcatgaaa cggggcgacc ggttttactc ataacgagaa ctgctgaagc
    10801 ggctgaatac ttttcaaaag tatttttcca aatggatatt cctaataatt tactcattgc
    10861 gcaaaatgtt gcaaaagaag cgcagatgat agctgaagca gggcaaattg gttccatgac
    10921 tgttgcgact agtatggcag gtcgaggcac agatattaaa cttggtgaag gtgtcgaagc
    10981 attaggtgga ttagctgtta ttattcatga acatatggaa aatagccgtg tagacaggca
    11041 attacgtggt cgttctggta gacaagggga tccaggatca tcttgtatat atatttcact
    11101 agatgattat ttagttaagc gatggagcga tagtaattta gcggaaaata atcaattata
    11161 ttcattagat gcacaacgat tatcgcaaag tagtttgttt aatcgcaaag ttaagcaaat
    11221 tgtagttaaa gcgcagcgta tctcggaaga acaaggggtt aaagctcgtg aaatggctaa
    11281 tgaatttgaa aaaagcatta gtatacagcg agatcttgta tacgaggaac gcaatcgagt
    11341 tttagaaata gatgatgctg agaatcgaga ttttaaagtg ttagctaaag atgtgtttga
    11401 aatgtttgta aatgaggaaa aggtgctaac aaaatcgcgt gttgtcgagt atatttatca
    11461 aaatttaagt ttccaattta ataaagatgt ggcttgtgtt aattttaaag ataagcaagc
    11521 tgtagtaaca tttttattag agcaatttga aaagcaaata gctttgaatc gtaaaaacat
    11581 gcaaagtgca tattattata atattttcgt tcaaaaagtc tttttaaaag caattgattc
    11641 atgttggtta gaacaagtcg actatttaca acaattaaaa gcaaacgtta atcaacgaca
    11701 aaatgggcaa cgtaacgcta tttttgaata ccatagagtt gcattagatt catttgaagt
    11761 tatgacacga aatatcaaaa aaagaatggt gaaaaatatt tgtcaaagca tgattacatt
    11821 tgataaagaa ggtatgccag ttatacattt tccataaacg aaaaggggta gcgcatgaca
    11881 atatataata taaactttgg tataggctgg gcgagtagcg gtgttgaata tgcacaagct
    11941 taccgtgcta aattattgag acgcacaaat caagacacta aatttgtgtt tttagatttt
    12001 atacagtcag agaacataca gacattaacg caaaacattg ggtttgaaga tcaagaagtt
    12061 atttggttgt atcagtattt tagtgatatt tctatagcac caacaacgta cacgttagat
    12121 gacattgaac gtgagcttgg tcatgatatt agttttagag aaagaaatgg taagatagtc
    12181 cgcctatatt ttaatgggaa atcatcattt gtaacatgtt atctgcaaaa tgaacaaaaa
    12241 gacattgtgg atcgtgcaga atttgtaatt aatagtatgt tagtacgtaa agatttttat
    12301 agttatacac gcatattttc tgagtattat gcaccagctg ataataaagc gaaattatat
    12361 atgcgacaat tctataatga aaatggctcc atcacttata acgaatatat tgatggagac
    12421 agctctatat atgtgtttga agatgcggtg ttgtataaca aaacggaatt tattgcatac
    12481 tttttacaac gattgaattt aacccgagac gatattgtca tattagaccg cgcttctgat
    12541 attggtcagg ctgttttgca gcataaaggt gatagtaaag ttggtgttgt gattcatgct
    12601 gatcattata gtaataatat gatgagcgag cagcatatac tatggaacaa ttattatgaa
    12661 tatcaatttt caaaagctaa atacattgat ttctttatta cagcgacaga tattcaaaat
    12721 catatggttt gtcgacaatt tgagcaatat caaggttatc gcccacgtgt ttatactatt
    12781 ccggtgggaa gcattgatgc attatcatat ccaacattat caagaaagcc atacgcgatg
    12841 attagtgcat ctcgtttagc aaatgagaaa catattgatt ggcttgtcaa agcagttatc
    12901 gtggctaaac gacaagtgcc agaattaaca tttgatattt atggagaagg tagtgagaag
    12961 acgcgactgc gtaaaattat tgatacacac cgcgcgcaag attatattcg tttactaggt
    13021 catgttaaac ttgatgaaat ttatactgat tatgagttat ttctttctgc atcaactagc
    13081 gaaggttttg gattaacact aatggaagcg gtaggctcag gactaggtat gattggatta
    13141 aatgtgaatt atggtaaccc aacctttatt cgagatgggg aaaatggcta cttggtacct
    13201 tttgataccg atgaagatag tgtggatgat gttgtcgcaa aactagctca tgccattgta
    13261 atgtatttta acaatggacc ccagacacca cattacatct cttatgaagt ggcacagcaa
    13321 tttatgactc aagatattat tttaaaatgg gaaacattag ttcaagaggt gctgcatgat
    13381 taaattattt gattacttta acggtcattc acgaaagtta tatgagtctt ttaaagccag
    13441 taagttggag gaagatttaa caattgtact caatgacaat ggttttttac cggacgatgt
    13501 tatatcaccg tatcaatttt ttgcagataa tcataataca gaaaatatga aaccacgatt
    13561 ttttaatcag gttactgtac cagctttttg ggaaattaaa ggtaacaaca attcggctac
    13621 aattaatgat atgggacgtc tacgaggcaa aatattttat caaagtggag agcgccctag
    13681 aattgttagt cgtgtcgaat ggtttgatga ccaacaacgt gtccgatttg tcgattatta
    13741 ttctaagaat ggtattaaat ttgcacaaac tgtttacgat ttaaatcgta aagcgatctt
    13801 gaagaaatat atgacagcag aaggtaaaga agtgatttat gaaaattttg taacatctga
    13861 tgtcatattg gattggcaag gaaagtctta ctttttccct tcaaagctcg cattcgtatt
    13921 attttttatc aagcaacttg aaattacgga acatcatttc gttattaact cactagccct
    13981 accattttca gtgttatata atttgccatc aaatggtagt gatgttttgg tatggcaaga
    14041 acagtgcgat ggtaatgtcc cgggaaatat gcaattgatg tgtaaaggtg atatgaaacg
    14101 acattgtaat attattattc cagataaaaa cgaatatgaa acgatgctca atatagcaga
    14161 cgcaaaggtt aaatctcgta ttttacaagg cggatatcta tataattatc gtagtcgtaa
    14221 tagatatact aaagaaattg taatattaac taactcggat caacttcgta acatcaaggt
    14281 attggttgaa actttaccag attttaagtt tcacatcgct gctattacag agatgtctga
    14341 taagctaatg caacttgatc aatatgctaa tgtgcactta tacccatcta ttaatataga
    14401 cagggtaaat gaactatacc agttatgtga tatttatctt gatattaatg agggtaatga
    14461 aattttaaat gcggtggaac aagcgtttga ctatgagttg ttgattttag gttatcgtca
    14521 aacagcacac catgctaaag taacactatc agagcactta tttgagcata atgatgagat
    14581 aacaatggaa agtaaagacc aattaataca aatgttagaa agtttgaaag atcaacaaca
    14641 gtttcgagat gctttattag cacaaaaagc acatgcacac gaaatctcgc gtgaacaatt
    14701 cgagcaagtt tttaaacagg cgttcgaaag ctaaaaagaa aaagcagtct aaaataaaac
    14761 atcatttaat ataaaagatt tgacgtaata tgatgttagc gacatggaca atatgatatt
    14821 acgtcatgct tgttttgttt acttaaatat aagctaagta ataagtagat aattactaac
    14881 aacaataact agatagataa gtgtaaattt cttgtaaaca ggtatataat agtatgtaat
    14941 tcttttaagg aagtaagtga gaaaggagta tgttgatggc taaatatcga gggaaaccgt
    15001 ttcaattata tgtaaagtta tcgtgttcga caatgatggc gactagtatc attttaacga
    15061 atatcttgcc gtacgatgcc caagctgtat ctgaaaagga tactgaaatt tcaaaagagc
    15121 tattatctaa gcaagattta ttagacaaag ttgacaaagc aaatcgtcaa attgagcaat
    15181 taaaacagtt atcggcttca tctaaagcac attataaagc acaactaaat gaagcgaaaa
    15241 cagcatcgca aatagatgaa atcataaaac gagctaatga gttggatagc aaagacaata
    15301 aaggttctca aattgaaatg aacggtcgaa gtgatataga cagtaaatta gatcaattgc
    15361 ttaaagattt aaatgaggtt tcttcaaagg ttgatagggg tcaacaaagt gacgaggacg
    15421 atcttaatgc aatgaaaaat gatatgtcac aaacggctac aacaaaacat ggagaaaaag
    15481 atgataaaaa tgatgaagca atggtaaata aggcgttaga agacttagac catttgagtc
    15541 agcaaataca caaatcggaa gattcagcag tgtctacaac agataataat catgaagtag
    15601 ctaaaacgcc aaataatgat ggttctggac atgttgtgtt aaataaattt ctttcaaatg
    15661 aagagaatca aagccatagt aatcgactca ctgataaatt acaaggaagc gataaaatta
    15721 atcatgctat gattgaaaaa ttggctaaaa gtaatgcctc aacgcaacat tacacatatc
    15781 ataaactgaa tacattacaa tctttagatc aacgtattgc aaatacgcaa cttcctaaaa
    15841 atcaaaaatc agacttaatg agcgaagtaa ataagacgaa agagcgtata aaaagtcaac
    15901 gaaatattat tttggaagaa cttgcacgta ctgatgataa aaagcatgct acacaacgca
    15961 ttttagaaag tatatttaat aaagacgagg cagataaaat tctaaaagat atacgtgttg
    16021 atggtaaaac agatcaacaa attgcagatc aaattactcg tcatattgat caactatctc
    16081 tgacaacgag tgatgattta ttaacgtcat tgattgatca atcacaagat aagtcgctat
    16141 tgatttctca aattttacaa acgaaattag gaaaagctga agcagataaa ttggctaaag
    16201 attggacgaa taaaggatta tcaaatcgcc aaatcgttga ccaattgaag aaacattttg
    16261 catcaactgg cgacacgtct tcagatgata tattaaaagc aattttgaat aatgccaaag
    16321 ataaaaaaca agcaattgaa acgattttag caacacgtat agaaagacaa aaggcaaaat
    16381 tactggcaga tttaattact aaaatagaaa cagatcaaaa taaaattttt aatttagtta
    16441 aatcggcatt gaatggtaaa gcggatgatt tattgaattt acaaaagaga ctcaatcaaa
    16501 cgaaaaaaga tatagactat gttttatcac caatagtaaa tcgtccaagt ttactagatc
    16561 gattgaataa aaatgggaaa acgacagatt taaataagtt agcaaattta atgaatcaag
    16621 gatcaaattt attagacagt attccagata tacccacacc aaagccagaa aagacgttaa
    16681 cacttggtaa agggaatgga ttgttaagtg gattattaaa tgctgatggt aatgtatctt
    16741 tgcctaaagc gggggaaacg ataaaagaac attggttgcc gatatctgta attgttggtg
    16801 caatgggtgt actaatgatt tggttatcac gacgcaataa gttgaaaaat aaagcataat
    16861 tatattgggg gaagtgcatc tatatatttt ttaagtatat aagacgtctt atttccccct
    16921 taaattattg tgaagtatat gcaaaatgca atgaatagat tgtccatcat tttaacgtta
    16981 taatgaattt aacgacttag aactacacaa gtaaaggaga atgaagatgt ctcgaaaaac
    17041 ggcgctatta gttttggata tgcaagaagg tatagcgagt agtgtaccta gaataaagaa
    17101 tattattaaa gcgaatcaga gagcaattga agcagcaaga caacatcgaa taccagtcat
    17161 tttcatacgt ttagtgttag ataagcattt taatgatgtc tcctcgagta ataaagtgtt
    17221 ttcaacaatt aaagctcaag gatatgcgat tactgaagca gatgcatcta cacgaatact
    17281 tgaagactta gcaccactag aagatgagcc gattatttct aagcgacgct ttagcgcatt
    17341 tacaggtagt tacttggaag tttatttacg tgcaaatgat attaatcatt tagtattaac
    17401 gggtgtctct acaagtggag ctgtattgag cacggcatta gaaagtgtag ataaagacta
    17461 ttatattact gttttagaag atgctgttgg tgatagatca gatgataaac atgactttat
    17521 tattgaacaa attttatcac gctcatgtga cattgaatcc gtagagtcat ggaaaagtag
    17581 tttatagtaa atataacgtc aattaaagct cggcagtaat gtttgagaat aagtacattt
    17641 gctcatattt ataaaatgtg tgagatggca attgaaacgg atatgttgag gaacattaga
    17701 acataaaata atatatttat ataaaatgac ccgaggcgtt cgaactgaat gcctcgggtt
    17761 taattgaata agaaatcgga cttatgaaca gaaatatgtt taagtccgaa ctccttgttt
    17821 atacttataa attttacggg tttaataata tttatttacc tgtaatatat gataattctt
    17881 cagcggcagc tgcattgata gttctatgag aaatgatacc taatccttta acattggatt
    17941 ctgaaataac gatagaacca tcactgttaa ctttttcaac aaatgctaca tgaccgtaat
    18001 gttgatctgc accaaattgt ccagcctcaa atacaacagc agcatgacgt tttggtgtat
    18061 gacttacttg ataatcacgg tattgagctc gattattcca attatgtgca tcacctaaat
    18121 cacctgagat agatgtacca aattgtttca tacggttata tacgtaccaa gtacattggc
    18181 catgtggata tggcatatta tcagatacct cgcggaaagg tttgaattca tctgatgaat
    18241 catcataatc cttgatagaa cgttcatatt tatctaaatc tggcatacgt tcatcgtcaa
    18301 actgagttaa ttgatagtgt ttaataatac tgtttaattt cttagcatag tttggatctg
    18361 tagcatatgt tttagataag tgtgatgttg catctttata agaatcggct tccgatttcc
    18421 atgttggttt ataaattgtt cgattgccat caataccatt tttaataagg tcagagtaat
    18481 cttttagtga ttctttcgtg cttggatatt ttcggaatcc agcattaata ctatacaatt
    18541 gattaccatc agcttctaat gtgttaaaag gaacagaatt cccttcaaaa gcacctttga
    18601 taccgaataa attatggttt ggtgacttag ctaaagcact acgacctgag tcagattcta
    18661 agattgcttg ggcaatcatg acagacgcat aaatatcgtt atcttgacca atgcggtgtg
    18721 catctttagc aattgatttg acaaattgac gtgtatcttt tgagtcaaca acgttaaatt
    18781 gtccactatc atcattgtta gatatactag gatctgtttc gaatagtgat gttgcacgtg
    18841 tatccttttg attaacatcg ttattgaatg attgagcagg tttagattta tgtttcaatt
    18901 catcttgtgt tggtaactgt ggattctttg tattagattt ttcatttttg tcttttttag
    18961 attgagatgc gtaatctttt tgtgttttct ttgcatcttc actgtattga tccaaaatag
    19021 aatctaaagc cgaatctgac attgattgat tatctttcga tgaagatttt tgatttactt
    19081 tatcgtcact tgctggttga ctatttgatt gatttggttg tgttggcttt ggcgaatttg
    19141 gttgcttatt agatgtactt ggttttgtat tgtttgattt aggtgctttt tgattgtctg
    19201 ctttatcttg tttagatgat tgcgtatccg tatcattttt gatgctatta tcactgtttt
    19261 tattcgaatc atttgttgac ttttcaccat tacgaggttg ttcgtaatca gaaatatccg
    19321 aatttaaatt gaataagttt tggattaaag ttgttaatga gtaattatca tcgtatttat
    19381 ttttggttag caattggttt atattggttt gtggtaaatt cttataaata aaatcaatga
    19441 tattgttaga gtctgaagtg ctgtcgtcta tagttttaac ttttttgtcg ttattgtctt
    19501 ggttactagt attattgttg tctgctttat caatatcttt acttgtagta tccttagaag
    19561 tttcatcatc agtagatttt ttggaatcat gagatgttgt cttagctgta gtatcttttt
    19621 gaggtgtgtc agcataagcg gtaggtgaaa ctaaagtagg taatacgagc gtagttgata
    19681 gcaaataaat taaaatttta tttttaggca tatttcgtat tctcccttga aaaatataat
    19741 aattaagtgt gataataaac tatgatttgt tataatttat cgtatgctga aaatagttga
    19801 taggtatcaa tcgactaaat atcttccagt aaattgatta tactaattca caacgcaaaa
    19861 ataaattaat ttacaaaaaa tatataaaaa atatgaataa ttcctacata ggagtgtgac
    19921 aatgaagaac gcatttaaat tatttaaaat ggatctgaag aaagtagcta agacgccagc
    19981 tgtgtggatt atcttagcag gcttagctat tttgccatcg ttctacgctt ggtttaactt
    20041 atgggcaatg tgggatccat atggcaacac gggacacatc aaggtcgcag tcgttaatga
    20101 agataaaggc gacacaatca gagggaaaaa agttaatgtc ggtaatacga tggttaacac
    20161 actcaagaaa aataaaagtt ttgattggca gtttgtaagt agagagaaag ctgatcatga
    20221 gataaaaatg ggtaaatatt ttgcaggtat ttacatccca tctaagttta cacatgaaat
    20281 taccgggaca ctacgtaagc agcctcaaaa agcagatgta gaatttaagg tgaatcagaa
    20341 gattaacgct gttgcgtcta agctaacaga tactggttcg tcagttgtcg ttgaaaaagc
    20401 gaatgaacaa tttaataaaa cagtaactcg agcattatta gaagaagcta acaaagcagg
    20461 tttaactatt gaagaaaatg tgccgacaat taataagata aaaaatgcgg tatattcggc
    20521 agataaagct ttacctaaga ttaatgactt tgcgaataaa attgtatatt tgaataacca
    20581 ccaagcggat ttagataaat atgcgaatga ttttagaaaa ctaggaaatt ataaaggtga
    20641 tattttagat gctcagaaaa aattaaacga agtcaatggt gctattccgc aacttaatga
    20701 aaaggctaag ttgatattag ctttaaataa ttatatgccg aaaattgaaa aagcgttaaa
    20761 ttttgcagct gatgacgtgc cagcgcagtt ccctaaaatt aatcaaggac ttaacattgc
    20821 gagtcaaggt attgatcaag ctaatggaca gttaaatgat gccaaaggct tcgtcacaca
    20881 agttagaagt agagtcggtg attatcaaga agcaattcga cgcgcgcaag atttgaatcg
    20941 aagaaaccag caacagattc ctcaaaatag cgcggcgaac aacgaaacat caaatagtgc
    21001 acctgcagct ggtaatggtg taacatcaac gccaccaagt gcaccaaatg gcaatactac
    21061 accaaataat aatgttacgc aaaataccgc accaaatagt aataatgcac ctgtatcgac
    21121 tacaccacaa agtacaagcg ggaaaaaaga tggtcaaagt tttgcagata taacaacaac
    21181 acaagtcagc acagctaacg agaacacaca aaatattaca gataaagatg ttaaatcaat
    21241 ggaagcggca ttaacgggct ctttattatc attatcaaat aatttagata cccaagcgaa
    21301 agccgcacaa aaagatagtc aggcattacg taatatttcg tatggcattt tagcatcgga
    21361 caagccatct gattttagag agtctttaga taatgttaag tccggtttag aatacacaac
    21421 tcaatataat caacaattta tcgatacatt aaaagagatt gagaagaatg aaaatgttga
    21481 tttatcaaaa gaaattgata aggtaaagac agctaataat cgaattaatg aatcattaag
    21541 gttagttaat caattaagta atgcattaaa gaatggtagt tcaggaactg ctgaagctac
    21601 taaattacta gatcaactgt caaaactaga ttcatcatta tcatcattta gagattatgt
    21661 taaaaaagat cttaacagct ctttagtatc aatatcacaa cgtattatgg atgaattgaa
    21721 caaagggcaa acggcattgt ctaatgttca gtctaagcta aatacaattg atcaagtcat
    21781 caacagtggt caatctattt taaaaaatgg taaaacacgt atcgatcgtt tacaaacagt
    21841 attaccaagt attgaacaac aatacattag tgctattaaa aatgctcaag caaacttccc
    21901 gaaagtgaaa agtgatgtag cgaaagcagc taactttgta cgtaacgatt taccacagtt
    21961 ggagcaacgt ttaaccaatg cgactgcaag tgtgaataaa aatttaccaa cgttattaaa
    22021 tggttatgat caagcggtag gattactaaa taaaaatcag ccacaagcga aaaaggcttt
    22081 atcagattta gctgattttg cacaaaataa attgccagat gttgaaaaag atctgaaaaa
    22141 agcgaataag attttcaaga agttagacaa agacgatgca gtcgataaat taatcgacac
    22201 acttaagaat gatttgaaaa agcaagcggg tattattgca aatcctatta ataagaagac
    22261 tgttgatgtt ttcccagtta aggattatgg atcagggatg acaccattct atactgcatt
    22321 atcggtatgg gtaggcgcac tcttaatggt aagcctatta actgttgata ataaacataa
    22381 gagcttagag ccagtgttaa cgacacgaca agtattctta ggtaaagcag gattctttat
    22441 aatgcttggt atgttgcaag cactcattgt atcggttgga gatttgttaa tcctaaaagc
    22501 aggagttgag tcacctgtat tattcgtact tataacgatt ttctgttcga ttattttcaa
    22561 ctcaatcgta tatacgtgcg tatcattact tggtaaccca ggtaaagcca ttgcaatcgt
    22621 attgcttgta ttacaaattg caggtggtgg aggtacattc ccaattcaaa cgacaccaca
    22681 atttttccaa aacatttcgc catacttacc atttacgtat gcaattgatt cattacgtga
    22741 aacagtaggc ggtattgttc cggaaatctt aattacaaaa ttaattatat taacgttatt
    22801 tggcatagga ttcttcgttg taggtttaat tttaaaacct gtaacagatc cattgatgaa
    22861 gcgcgtatct gaaaaagttg accaaagtaa cgttacagaa taaaaattaa atccacacat
    22921 tagggttata gctccttaat gtgtggattt ttatgttttt agacagaaga gatagtaatt
    22981 tctgtctttt atgggacggg tgttatcatt gctattatcc aggatgactt actatagcac
    23041 taatattacc gacaaagtga atatcctcat cttccgtagt taaaataaag ctagaacctt
    23101 tttggatgtc atagtgctta tcgtttactg ctaaagtgcc agtaccatcg ataattgtaa
    23161 ctaagcaata agcatgtggt ttattgaatt ttaaatctcc atgaatatcc catttatata
    23221 ctgcaaaata ttgattatct acaaattgag ttacagtgtg tgtgtcgatg tgagttgtta
    23281 taggagtagt atttggttca tgattgccta attcaatcac atctttactt tgctctaagt
    23341 gcaaatcacg caattgacca ttttgatctc gtctatcata gtcataaata cggtatgtcg
    23401 tatcggatga ttgttgtgtc tctaaaatta aaatacccga accaatggca tggacagtgc
    23461 cagcaggaac ataataaaag tcaccggact taacaggtat acgtttgaaa agactgtcaa
    23521 attcatgatt atcaatcatg tcggttaacg tttgtttatt atgtgcatgt acgccatata
    23581 taatttcagc acctggggct gcatctaaaa tataccaaca ttctgtttta cctagttcgc
    23641 cttcgtgttt taaagcgtaa tcatcatccg gatgaacttg aacagataat ttatcattgg
    23701 catctaatac tttagttagc agagggaaac tatctcgtga atcattatcg aataattcac
    23761 gatgttgtga ccaaagttga tctagggtca tatccttgta tggaccattg ataattgtat
    23821 taggaccatt tggatgtgca gaaattgccc agcattcacc agttgtttca ttagggatat
    23881 catagttaaa tgcttttaat gcatgaccgc cccaaattct gtctttaaaa acgggttgta
    23941 aaaataatgc catagttaaa actcctctat attttcatta ataagttata aatttctgta
    24001 gtactgtttg cattaattag tgattggcgc gtctcatcat tcattaacgc tttagataag
    24061 cgctgaagta tttttaaatg tgtatcctga ctgttgtttg gtacggcaat taagaaaatc
    24121 aattgaggta gactaccatc tagactttcc catttaacac catgattatt tttcataaca
    24181 gctacaatcg gttgttttac aacatcagac tttgcatgtg gaatggccac gttcatgcca
    24241 atagctgtcg tagactccat ttcacgttct aggattgcat tttttaaatg cgatgtgtgc
    24301 tctacataac ggcacacttt aagttgatga atcaacgtat cgattgcttc gtttcgagac
    24361 atgttatgat cagcaataat gatagtttgt tgatcaaaca catgagaagg tttattgaga
    24421 tgtgaatgtt tcgcggtgtt atctacattg tcaacctctg tatcatgttg tgttatatct
    24481 gtatcatgaa gttgcgtatg ttgcgctggt gcatctactg ctataactgg tgtgcgacgt
    24541 tttaataata gtacagtaat cattgtgaca agactaccta ctatcactgc aaagataaac
    24601 cataatatat gatcaatacc acctaataca gccactattg gacctccatg tgccactcta
    24661 tctccaacac caccgatggc tgcaatgact gatgcaatca tcgctccaat catgttagca
    24721 ggtataatgc gtagtgggtc ttgagctgcg aaaggaatag caccttcagt aataccaaat
    24781 agtcccatag tgaaggaagc cttacccatt tctctttcgg aatgattgaa tttatacttt
    24841 tgaacaaacg ttgctaaacc aagaccgatt ggtggtgtac atacagcaac tgcgaccata
    24901 cccataacgg cgtaattacc ttcagcaata agtgctgagc caaataaaaa tgctactttg
    24961 tttactggac cgcccatatc gaaggcgatc atcgcaccta ttatcatcgc aagtataata
    25021 atattagcac cttgcatact ttttaaccag gttgttaatg cctcaaaaat attagaaatt
    25081 ggtgcaccga ttaaaaatat aaatatcaat cctacaacga ccgatgaaat aatgggaata
    25141 ataatgatag gcataattgg tgccatcgct tttggaactt taatatcttt aatccacttt
    25201 gcgatataac ctgctaagaa accagcaaca ataccaccta aaaatcctgc gcctgcatca
    25261 ctgccataaa aactaccgtc agcagcgata gcgccgccaa tcataccagg aacaagaccg
    25321 ggcttgtcag cgatactaac agcgatatat ccagctagta ttgggaccat aaatttaaag
    25381 gctaaactac caatgttttc aatggatttc caaaatgaat catccggtat gactaatcct
    25441 tttgatgtcg tttcaccgcc tagagtcagc gcgatggcga taaggagtcc accaactacg
    25501 ataaaaggaa ccataaacga tacaccgttc attaaatgtt gatacaccat ttgaatacca
    25561 cttttagact taccgcgatc tttcgaatga taatttgttt cagattgata aataggcgca
    25621 tcttgattaa tgatacgttg aattagacct ctcggattat gaatcccttc gcgaacattt
    25681 tcattaatca accgtttacc aacaaatcgg gacagatcaa cttgtttatc agctgcaatt
    25741 atgacaccgt cagcttcttc gatgtcttgc gtagttaaaa cattttcagc accaacaccg
    25801 ccctgtgtct ctactttaat atccacaccc atttcttttg ctacctgctc aagtttttct
    25861 tgagccatat atgtatgtgc aatgccattt gggcatgagg taatagctac aattttcata
    25921 aaatcatctc cttttccata ttgtaagcgt attcttgatg ctaaaaaaaa gaataattac
    25981 cgttactagt ggcaattatt cttgtaagta ttcaaataac tgttgcttta aactatgatt
    26041 atctaaacta cataaatggt tcactgaatc atcatccaag ttagcaatta attgcatcat
    26101 ttgttttgta aaagctgtgt ctttttgtga aattgctaag aaaaagacga gtttgacatc
    26161 gtgttgtcgc catggaaaaa catcttttgt gcgaaaaata agcacatgtg attgtaaaac
    26221 tttttcagga tctccatgag gaatcgccat aaaattacct atgtatgtag aagatgattt
    26281 ctcacgctct aaagctgatt cgatatatcc ttctacaatc gcatgatgtg cttgtaatat
    26341 tttttgagct tcttcaaaaa tttgcacagt atgtcgtggt ttttgttcag tatttacgac
    26401 aaggaaatta acagtgtcca tatgatgatg tgcttgaacc ggattttgct tttgcttcac
    26461 aacgtgtatg attttgtgac ggtcatcctc agaaaataat ggtgcaacct tgatagttga
    26521 aaggtgctta gggagcattt gtaaagtttg ctttgggata tcgtgagttg taattagtaa
    26581 atcaacattg tcaaagtgat agtgtgttat attttctagt ttaatcgtat ttatcactga
    26641 caactcttcg gataagttat tattttagtt tctaaaaaat tcgacacacc tagaccataa
    26701 taacaagcaa tgactacatt taattgtgtt ttggtacgac gctcgatggc agcttgaaaa
    26761 tgaattgtta aaaaggcaat ttcatcttcg ctcatctcta tatcagtatc aattgctaat
    26821 ttatcaatcg cttcaaaaag tgtgttgaac acaaagggat aaagtttttt aatctctata
    26881 actaaaggat tgtttaaata aatgttttga gtgatacgta aatatgcttt actaaaatga
    26941 ttatataaat tttgttgtaa aatcgaatct tcattgaaag gtacatgaat acgttgctgc
    27001 atcaattcga ttaagcgatc aatataactt tgtataaata tacgttctat gccaatatcg
    27061 agtttattaa aatgataagc aataaagaat gaaaacatat tgattacttt ttcattcaag
    27121 tcataaccta atctttcgtt gatttgctta atgcaagatt gagatatcaa ttttgcatcg
    27181 tggtttaacg tgtctagctc acttaaagat tgcttacgtt taatgattaa aatgagatga
    27241 attaaaagct gttgtatttg aatatcagtt gtttcaatac tatgttgttg aagtgtctct
    27301 tgtataatat gcgaaatcat cctttggtgt gaatcaggta attcatttaa aattaggtct
    27361 tcaacatgta catgccctga tgataattga tttaaatgga tgatggcatt agtgatatca
    27421 ttatctgttc catcgaccac aataccttgt tttttcttaa ttgttaaagc gatattaaat
    27481 ttttgacacc attgttgaat tcgaacgaaa tagtcgttga gcacagtttt agataataga
    27541 taatcttgct ctagttgttg caatgtctgt gatttattgt gcattagcag ttgatacgcc
    27601 atagataata atagtttttc tttatatgat tgaatatgcg tgtaacgttc agttatcgtt
    27661 tctaaggtat actgcgatgt atttaattga tatcctttag atttaatact tacgatgata
    27721 tcatccataa aattgctatt gataacatgg atatcattgc gaactgtgcg gtttgaaaca
    27781 ttgacatgtt cagcaatttc attagaacta atgtgctttg atgggttttt aataaaaaac
    27841 tggagtagtt ttaagtgtct atcaagcatt taacatgtac ctcctttcta aatttttcgt
    27901 gtaagcgttt ttaaatgtgt attaatatta ttaaacatga gagcttatac atacgtcaat
    27961 gacattaaag cgaactttta tatgaattta acaaagtgag aattatgcaa ataaagaaca
    28021 gcagtaagat atttcaaata gaaaaatatc tcattgctgt ttttctgaat ttatgtacct
    28081 gatacttgtg gacgtatcag gtaaatgatt aatttttaga tgattgcggt tgtgatggtg
    28141 atgcctgctt agaagactcg tttgtattag cattattaga tttgtttggg gccggtgtat
    28201 tctgttgttt atgattttgg ttattcgttg aaggttgact gctaccgccc tgatttgaat
    28261 ttgattgatt tgaatttgat tgattcggat tagatggttg tgtattcgct ggtgctgttg
    28321 aaccgttatt ttggacagtt gatggcgtag atgattgatt gccattagta acaccgttgt
    28381 tgctgttttg attttcattt gtgttgtttg tattctcgtc tttttgatga tttacttcag
    28441 tatctttctt ttgatggtta gctttattat cttgctcatt attatgattt gatgatgtag
    28501 aatgatttat tgcagcataa atagcgaatc caagtgcacc aattaacaag atgataacaa
    28561 cagtaataag aaattttttc aaatgtaacg cctccgttta taataaaaat agtcatacat
    28621 tgcatggcat catgctttca atgtcgataa aatgatgtta actataaaac ttgaaatgcg
    28681 gacgatatta atatttgtgt cagtttttta tgtacttcaa ataagtataa caaaactgtt
    28741 atatatttat aaatataatc aatagtattt tattgtacaa gcaagttata ataatagtaa
    28801 taagattgaa aaatttgtat ggaaaagaga gataaatatg aatcaaacaa ctaaactttg
    28861 tgcagcaacg acattagcat taggtacttt aattggcgca acagttgtag ggactgcatc
    28921 accaatacat caagaagcac aggcggcaac aacaccttac tatacttatc atggatatat
    28981 cggtcataac gctaatttta taatggataa gcattttatt aacgctatta aatatgacaa
    29041 cgtgacattt aatggtatta aattagcgaa gacaaatgcg acaaaaaaag tagagaaata
    29101 cgatcaaact tttaaaggtg taactgcaaa aggaaatgaa gcgaatcaat tacaatttat
    29161 cgttaaaaat aatatatcac taaaagatat tcaaaaagct tatggtaaag atttgaaaaa
    29221 agagaataat aaaacgaaag aatctgatag cggtattttt tattatcaac attctaaaaa
    29281 gtcattaggc atttggtttg ttgttgatca taatagagtg gttgaagtta cagtgggaca
    29341 tacgccatac aaaacgagta aataataaag taaatatagg ggctagtatg tgaggaatga
    29401 tgtcacatac tagtcttttt tgattatatg atggtagttt tcgtgtctcc atgatattga
    29461 aatatataga tttaatcatg cgcagttaag tagtcagcat atatatttca catgttgatt
    29521 aagtatgtgt tgtactaatt ttttgattgt taataatttc acaaaaataa agccttgata
    29581 aattttaaaa gacaattaag tgcaattttc aaaattttat ttagtcacag ataacatttt
    29641 taaaaaagaa aagaatcaat aaataaaatc aacgaacaaa aagtatagaa ataaatagaa
    29701 ataatcgttt acttttctga aaaattaaat taatatttta tttataagct gtttttaaaa
    29761 tttcaggagg aatgaaatgt gaggaaattt tcaagatatg catttacaag tatggcaaca
    29821 gtaacgttgc tgagctcttt gacacctgca gcactagcga gtgatacgaa tcacaaacca
    29881 gcaacttcag atattaattt tgaaatcacg caaaagagtg atgcagttaa agcattaaaa
    29941 gagttaccta aatctgaaaa tgtgaaaaat cattatcaag attactctgt tacagatgta
    30001 aaaacagata agaaaggatt cacgcattac acgttacaac cgagtgtgga tggtgtgcat
    30061 gcgcctgaca aagaagtgaa agtgcatgcg gacaaatcgg gtaaagtcgt tttaatcaac
    30121 ggtgatactg atgcgaagaa agtaaagccg acaaataaag tgacattaag caaggatgaa
    30181 gcggctgaca aagcatttaa cgcagttaag attgataaaa ataaagctaa aaacctccaa
    30241 gatgacgtta tcaaagaaaa taaagtcgaa atcgatggtg acagtaataa atacatttac
    30301 aatattgaat taattacagt aacaccagaa atttcacatt ggaaagttaa aattgatgca
    30361 gacacaggag cagttgttga aaaaacgaac ttagttaaag aagcagcagc aactggcaca
    30421 ggtaaaggtg tgcttggaga tacaaaagat atcaatatca atagtattga tggtggattt
    30481 agtttagagg atttgacgca tcaaggtaaa ttatcagcat acaattttaa cgatcaaaca
    30541 ggtcaagcga cattaattac taatgaagat gaaaacttcg tcaaagatga tcaacgtgct
    30601 ggtgtagatg cgaattatta tgctaaacaa acatatgatt actacaaaaa tacatttggt
    30661 cgtgagtctt acgataacca tggtagtcca atagtctcat taacacatgt aaatcattat
    30721 ggtggacaag ataacagaaa taacgctgca tggattggag acaaaatgat ttatggtgat
    30781 ggcgatggcc gcacgtttac aaatttatca ggtgcaaatg acgtagtagc acatgagtta
    30841 acacatggcg tgacacaaga aacggcgaat ttagagtata aagatcaatc tggtgcgtta
    30901 aatgaaagct tttcagatgt ttttggatac tttgtagatg atgaggattt cttgatgggt
    30961 gaagatgttt acacaccagg aaaagaggga gatgctttac gaagcatgtc aaacccagaa
    31021 caatttggtc aaccatctca tatgaaagac tatgtataca ctgaaaaaga taacggtggt
    31081 gtgcatacga attctggcat tccaaataaa gcagcttata acgtaattca agcaataggg
    31141 aaatctaaat cagaacaaat ttactaccga gcattaacgg aatacttaac aagtaattca
    31201 aacttcaaag attgtaaaga tgcattatac caagcggcta aagatttata tgacgagcaa
    31261 acagctgaac aagtatatga agcatggaac gaagttggcg tcgagtaaaa atatataaac
    31321 aagaagaagt aatgttaaac acttataaat aattaaattt taaatacagc ttatataagg
    31381 gttataccta cattgataat tgactttgta ggtgtaccct ctttttgtaa ttagatgaga
    31441 ggagtatgag ggttgtctgc cgaaagacta ctcggcagtc taaaatcatt actagtagta
    31501 aatatgtgat aattaaatgc tgacttagaa tacaaaattc attttaaaag ttgtcacaaa
    31561 aaatttacat gtatttttat tatcttttgc aaaacaaagt gttaaattat aaatgaaaca
    31621 tgcatgaatt tattttttaa tacaagaaac gtaactacca aaggagttta caatatgaag
    31681 aaaagtaaac gattagaaat tgtttctaca atagttaaaa agcataagat ttataaaaaa
    31741 gaacaaatca tttcatatat tgaagaatat tttggtgtaa gatatagtgc aacaacaatt
    31801 gctaaagact tgaaagaact taatatatat cgtgtcccta tcgattgtga gacatggatt
    31861 tataaagcta ttaataatca aacagaacaa gagatgagag aaaagtttaa acattattgt
    31921 gaacatgaag tacttagctc gattatcaat ggcgcatata ttatagtcaa aacctcacca
    31981 ggtttcgctc aaggcattaa ctactttatc gatcaattaa atattgaaga aatattaggt
    32041 acggtgagtg gaaatgacac tacattaatc ttaactgcat caaatgatat ggcagaatac
    32101 gtatatgcaa aattatttaa atagacatgt atcaaatgaa taataaaaat ttgttttgta
    32161 tcacgtgtac acaagttagt tactacatat taacttgtgt acgcgttttt ttatggaaag
    32221 aaagaattca tagtcattca tttgactgta taaaaaactt tatacaacat gtttttgtgg
    32281 gtgtttttga ataaaaaatg tatattttaa cccaaaatac ctttatttat gtataaaaat
    32341 ccattattat gtattgtata acaaaaagat atgaaatttt cgactttctt tatgtgaata
    32401 taatcacatg taagcgtttg aagattgtct atactctaaa tgaattcaaa gataaaagga
    32461 ggaaatagac atgacagatg gtccaattaa agtaaatagc gaaattggag ctttaaaaac
    32521 tgtgttactt aagcgtcctg gaaaagaatt agaaaattta gtgcctgatt atttagatgg
    32581 attactattt gatgatattc catatttaga agtagctcaa aaagagcatg accattttgc
    32641 acaggtgcta agagaagagg gtgttgaagt actttacctt gagaagttag cagctgaaag
    32701 tattgaaaat cctcaagtaa gaagtgaatt tattgatgat gtattagcag agtctaaaaa
    32761 aacaatatta ggtcatgaag aagaaattaa ggcattattt gcgacacttt ctaatcaaga
    32821 acttgtagat aaaataatgt caggagtacg taaggaagaa attaatccga aatgtacaca
    32881 tctagtagag tatatggatg ataagtatcc attctattta gatccaatgc caaaccttta
    32941 ttttactaga gatccacaag cctcaatagg acacggtata acaatcaatc ggatgttctg
    33001 gagagcacga cgacgagaat caatatttat tcaatatatt gtaaagcatc atcctagatt
    33061 taaagatgcg aatattccaa tctggttaga tcgagattgc ccattcaata ttgaaggcgg
    33121 cgatgaactt gttttatcta aagaagtctt ggctataggc gtttcagaac gtacatctgc
    33181 acaagctatt gaaaagttag cgcgacgtat ttttgaaaat ccgcaggcga cgtttaaaaa
    33241 agtagtagca attgaaattc caactagtag aacatttatg cacttagata cagtatttac
    33301 aatgatagat tatgacaaat ttacaataca ttcagccatt ttaaaggcag aaggcaatat
    33361 gaatatattt attattgaat atgatgacgt aaataaagat atttccatca aacaatctag
    33421 tcatttaaaa gatactttag aagacgttct aggtatagat gatatccaat tcattccaac
    33481 aggaaatggt gatgtcattg atggtgctag agagcaatgg aatgatggct caaatacatt
    33541 atgtataaga ccaggtgttg tagtgactta cgatagaaac tatgtatcga atgatttatt
    33601 gagacaaaaa ggtataaaag tcattgaaat atctggtagc gagttggtac gtggacgtgg
    33661 gggtcctaga tgtatgagtc aaccactatt cagagaagac atttaacgta ataaattata
    33721 gaagcgaggg tgaaaataat gacagaaatt caaaaaccgt atgatttaaa aggcagatca
    33781 ttattaaaag aaagtgattt taccaaagca gaattcgaag gacttattga ttttgcaatt
    33841 acattaaaag agtataagaa aaatggtatc aagcatcact acttatctgg aaaaaatatt
    33901 gcactactat tcgaaaagaa ttcgacgaga acgcgtgcag cgtttacagt tgcgtctatt
    33961 gatttaggtg cgcatccaga atttttaggg aaaaatgata ttcaattagg caaaaaagaa
    34021 tctgtagagg atactgcgaa agtattaggt agaatgttcg atggtattga attccgtggt
    34081 ttttcacaac aagctgttga agatttagcg aagttctctg gtgtaccggt gtggaatgga
    34141 ttaacagacg attggcatcc tacacaaatg ttagctgatt ttatgacaat aaaagagaat
    34201 tttggatatc tggaaggaat aaacttaact tacgttggag atggacgtaa taatattgcg
    34261 cattcattaa tggtagcagg tgctatgtta ggtgttaatg taagaatttg tacacctaaa
    34321 tcattaaatc caaaagatgc atatgttgat attgcaaaag aaaaagcgag tcaatatggt
    34381 ggttcagtca tgattacgga taatattgca gaagcagttg aaaatacaga tgctatatat
    34441 acagatgttt gggtatcgat gggtgaagaa agtgaatttg aacaacgtat taatttatta
    34501 aaagactatc aagtgaatca acagttgttt gatttaacag gtaaagattc aacgatattc
    34561 ttacattgtt taccagcatt ccatgataca aatacacttt atggacaaga aatttatgaa
    34621 aaatatggat tagctgaaat ggaagttaca gaccaaatct ttagaagtga acattcaaaa
    34681 gtgtttgatc aagctgaaaa tagaatgcat acaattaagg cagtaatggc agcaacattg
    34741 gggagttaat cactaaatgg aacgatatga atatgatgtg tctgatgata taagtatcaa
    34801 gtacagacac ctcatattgg tattaaagga gaaatgaata tgaacgaatc aggagataac
    34861 aaactcagta aatcttcttt aattggacta gttataggat ccatgattgg tggcggtgcg
    34921 ttcaatataa tgtctgatat gggtggtaaa gccggtggat tagccattat tattggttgg
    34981 attattacag ctataggaat gatttcatta gcgttcgtat ttcaaaattt aaccaatgaa
    35041 cggccggagc tagacggtgg tatttatagt tatgctcaag caggatttgg cgattttgta
    35101 ggctttatca gtgcttgggg atattggttc tcagcatttt taggcaatgt tgcctatgca
    35161 acactattga tgtcagcagt tggtaacttt ttcccgattt ttaaaggagg caacacatta
    35221 ccaagtatta ttgtcgcctc gttactactc tggggtgtgc atttcctaat tttaaaaggt
    35281 gttgaaacag cagcctttat caatagtatt gttactgttg caaagttaat accgatttta
    35341 cttgtaatca tatgcatgat aattgcattc aattttgaca cttttaaaac aggctttttc
    35401 ggtatgacgt cagagggtgt attgccattt agttgggcga gcacaatgag ccaagttaaa
    35461 agtacgatgc tagtgacagt ttgggtgttt atcggtatcg aaggtgcagt aattttttct
    35521 agcagagcta aaaataaaaa agatgtaggt agtgccacgg ttataggact tatatcagtt
    35581 ttaattatct atttcttatt aactgtatta gctcaaggcg tgattttgca aaatcatatt
    35641 tctcaattag attcgccaag tatggcacag gtgcttgcat ctattgtagg tggttgggga
    35701 tctacacttg taaatattgg tttaattatt tcggtactag gtgcatggtt aggatggaca
    35761 ttgcttgctg gtgaattacc tttcattgtt gcaaaagatg gattatttcc aaaatggttt
    35821 gctaaagaaa ataaaaatgg agcacctgta aatgcactgc ttattaccaa tatattagta
    35881 caattatttt taataagtat gctatttaca cagagtgcgt atcaatttgc attttcacta
    35941 gcatcaagtg ctattttata cccttacatg ttcagtgcat tttaccaagt taaatacact
    36001 ttagagcatc gacagcaagc aactactaaa caatggacga ttggtatcat agcctcaatt
    36061 tatgctatat ggcttatata tgcagcaggt atcaattact tattactgac gatgttactt
    36121 tatattccag ctcttcttgt ttattcaatc gttcaaaaga ataatcagac acgtttgatt
    36181 aaatcagact atattctttt tatgattatt atcgtacttg cagttatcgg gttaattaag
    36241 ttattgatgg gaacgataaa tgttttttaa aaggagcgac aaaaatatga aagagaaaat
    36301 tgtcattgca ttaggcggta atgcgataca gacaaaagaa gcaacagctg aagcacaaca
    36361 aacagctatt agacgtgcga tgcaaaacct taaaccttta tttgattcac cagcgcgtat
    36421 tgtcatttca catggtaatg gtccacaaat tggaggttta ttaatccaac aagctaaatc
    36481 gaacagtgac acaacgccgg caatgccatt ggatacttgt ggtgcaatgt cacaaggtat
    36541 gataggctat tggttggaaa ctgaaatcaa tcgcatttta actgaaatga atagtgatag
    36601 aactgtaggc acaatcgtaa cacgtgtgga agtagataaa gatgatccac gatttgataa
    36661 cccaactaaa ccaattggtc ctttttatac gaaagaagaa gttgaagaat tacaaaaaga
    36721 acagccaggc tcagtcttta aagaagatgc aggacgtggt tatagaaaag tagttgcgtc
    36781 accactacct caatctatac tagaacacca gttaattcga actttagcag acggtaaaaa
    36841 tattgtcatt gcatgcggtg gtggcggtat tccagttata aaaaaagaaa atacctatga
    36901 aggtgttgaa gcggttatag ataaagattt tgctagtgag aaattagcaa cgctgattga
    36961 agcagatacc ttaatgattc ttacgaatgt agaaaatgta tttattaact ttaatgaacc
    37021 taatcaacaa caaatcgatg atattgatgt agcaacactg aaaaaatacg cggcacaagg
    37081 taagtttgcg gaaggatcga tgttgccaaa aatagaagct gcgatacgat ttgttgaaag
    37141 tggggaaaac aaaaaagtta tcattaccaa tttagagcag gcatacgaag ctttgattgg
    37201 taataaaggt acacacattc acatgtagca ctttatcacg cgacaaaaca ttaaatatgt
    37261 ttctccgttg attcaaatga aaaagttgtc tgctgacact ttgcaaggtt tgaaggagtt
    37321 taacttatga cagaaaactt tattttgggt agaaataata aattagaaca tgaactaaag
    37381 gcattagcag attacattaa tatcccatat agtatattac aaccatatca aagtgaatgt
    37441 tttgtcagac attatacgaa aggccaagtt atttattttt cgccacaaga aagtagcaat
    37501 atttactttt taattgaagg taacattatt agagaacatt acaatcaaaa tggagatgta
    37561 tatcgttatt ttaataaaga gcaagtatta tttccaatca gtaacttatt tcatccgaaa
    37621 gaggttaacg aattgtgcac agcattaacc gattgtacag ttcttggatt gcctagagaa
    37681 ttgatggcct ttttgtgcaa agctaatgat gatatatttt tgacactttt tgcattaata
    37741 aatgataatg agcagcaaca catgaactat aacatggcat taacaagtaa atttgctaaa
    37801 gatcgaatta tcaaattgtt atgccatcta tgtcagacag taggatacga tcaagatgaa
    37861 ttttatgaaa tcaaacagtt tttaactatt caactcatga gtgatatggc tgggatttcc
    37921 cgggaaacag ctggtcatat tattcatgaa cttaaagatg aaaaacttgt tgttaaagat
    37981 cataaaaatt ggttagtaag caaacattta ttcaatgatg tatgtgttta atatacaatg
    38041 taaaatgaat aagttgaaca tgaggtctaa cgtacattta tacgttaggc cttttttgtt
    38101 agcatgatga ttaatttaat atgttagtta aatttgattg ttgaaattac agtaaaattt
    38161 aaggtgatga aaaatttaga acttctaagt ttttgaaaag taaaacattt gtaatagtgt
    38221 aaaaatagta tattgatttt tgctagttaa cagaaaattt taagttatat aaataggaag
    38281 aaaacaaatt ttacgtaatt tttttcgaaa agcaattgat ataattctta tttcattata
    38341 caatttagac taatctagaa attgaaatgg agtaatattt ttgaaaaaaa gaattgatta
    38401 tttgtcgaat aagcagaata agtattcgat tagacgtttt acagtaggta ccacatcagt
    38461 aatagtaggg gcaactatac tatttgggat aggcaatcat caagcacaag cttcagaaca
    38521 atcgaacgat acaacgcaat cttcgaaaaa taatgcaagt gcagattccg aaaaaaacaa
    38581 tacgatagaa acacctcaat taaatacaac ggctaatgat acatctgata ttagtgcaaa
    38641 cacaaacagt gcgaatgtag atagcacagc aaaaacaatg tctacacaaa cgagcaatac
    38701 cactacaaca gagccagctt caacaaatga aacacctcaa ccgacggcaa ttaaagatca
    38761 agcaactgct gcaaaaatgc aagatcaaac tgttcctcaa gaagcaaatt ctcaagtaga
    38821 taataaaaca acgaatgatg ctaataacat agcaacaaac agtgagctta aaaatcctca
    38881 aacattagat ttaccacaat catcaccaca aacgatttcc aatgcgcaag gaactagtaa
    38941 accaagtgtt agaacgagag ctgtacgtag tcttgcagtt gctgaacctg tagtaaatgc
    39001 tgctgatgct aaaggtacaa atgtaaatga taaagttacg gcaagtgatt tcaagttaga
    39061 aaagactgca tttgacccta accaaagtgg taacacattt atggcggcaa attttaaagt
    39121 gactggacaa gtgaaatcag gggattattt tacagcgaag ttaccagata gtgtaactgg
    39181 taatggagac gtggattact ctaattcaaa taatacgatg ccaattgcag acattaaaag
    39241 tacgaatggc gatgttgtag ctaaagcaac atatgatatc ttgactaaga cgtatacatt
    39301 tgtctttaca gattatgtaa atgataaaga aaatattaac ggacaatttt cattaccttt
    39361 atttacagac cgagcaaagg cacctaaatc aggaacatat gatgcaaata ttaatattgc
    39421 ggatgaaatg tttgataata aaattactta taactatagt tcgccaattg caggaattga
    39481 taagccaaat ggcgcgaaca tttcttctca aattattggt gtagatacag cttcaggtca
    39541 aaacacatac aagcaaacgg tatttgttaa ccctaagaaa cgagttttag gtaatacgtg
    39601 ggtgtatatt aaaggttacc aagataaaat cgaagaaagt agcggtaaag taagtgctac
    39661 agatacaaaa ctgagaattt ttgaagtgaa tgatacatct aaattatcag atagctacta
    39721 tgcagaccca aatgactcta accttaaaga agtgactggt gagtttaaag ataaaatttc
    39781 atacaaatac gataacgtag caagtattaa ttttggtgat ataaataaaa cgtatgttgt
    39841 attagtggaa ggtcactatg ataatactgg taaaaacttg aaaacacagg ttattcaaga
    39901 aaatattgac ccagcgacag gtaaagacta cagtattttc ggttggaata atgagaatgt
    39961 tgtacgttat ggaggcggaa gtgctgatgg tgattcagca gtaaatccag ttgatccaac
    40021 accgggacca ccagttgacc cagagccaga accggagcca acgccggatc cagagccaag
    40081 tccagagcca gaaccggaac caacgccaga tccagagcca agtccagaac cagatccgga
    40141 ttcggattca gatagtgact caggctcaga cagcgactca ggttcagata gcgattcgga
    40201 ttcagatagt gactcagact cagatagtga ctcaaactca gacagtgagt cagattcaga
    40261 cagtgactcg gactcagata gtgactcaga ttcagatagc gattcagaat cagacagcga
    40321 ttcggactca gacagtgagt cagattcaga tagcgactcg gattcaaaca gcgattcgga
    40381 ctcagacagt gactcggact cagatagtga ctcggactca gatagtgact caaactcaga
    40441 tagtgactca gattcagaca gcgattcgga ttcagatagc gattcagatt cagacagcga
    40501 ttcagaatca gacagcgatt cggattcaga cagtgactcg gattcagata gcgactcgga
    40561 ttcagacagc gattcggact cagacagtga ctccgattca gatagcgatt cagaatcaga
    40621 cagtgagtca gattcagata gcgattcaga atcagacagt gactcagacc cagacagtga
    40681 gtcagattca gacagcgatt cagactcaga cagcgattca gaatcagaca gtgagtcaga
    40741 ttcagacagc gattcggact cagatagcga ctccgattca agagttacac caccaaataa
    40801 tgaacaaaaa gcaccatcaa atcctaaagg tgaagtaaac cattctaata aggcatcaaa
    40861 acaacaccaa actgatgctt taccagaaac aggagataag agcgaaaaca caaatgcaac
    40921 tttatttgat gcaatggtgg cattattagg atcattacta ttgtttagaa aacgcaagca
    40981 agatcataaa gaaaaagcgt aaatactttt ttaggccgaa tacatttgta ttcggttttt
    41041 ttgttgaaaa tgattttaaa gtgaattgtt taagcgtaaa atgttgataa agtagaatta
    41101 gaaaggggtc atgacgtatg gcttatattt cattaaacta tcattcacca acaattggta
    41161 tgcatcaaaa tttgacagtc attttaccgg aagatcaaag cttctttaat aacgatacaa
    41221 ctgctaaacc attaaaaact ttaatgttgt tacatggatt atcaagtgat gaaacgacat
    41281 atatgagata tacaagcata gaaaggtatg cgaatgaaca caaattagct gtgattatgc
    41341 ccaatgtgga tcatagcgca tatgctaaca tggcatatgg tcatagctat tatgattata
    41401 ttttggaagt atatgattat gttcatcaaa tatttccact ttccaaaaaa cgtgatgaca
    41461 attttatagc aggtcactct atgggaggat atggcacaat taaatttgca ttaacacaag
    41521 gggataaatt tgccaaagct gtaccattat ctgctgtgtt tgaagcgcaa aatttaatgg
    41581 atctagagtg gaatgatttt tcaaaagagg ccataattgg caatctttca agtgttaaag
    41641 gaactgaaca tgatccgtat tacttgctag acaaagctgt agctgaagat aaacaaattc
    41701 caaaattgct cattatgtgt ggtaaacaag actttttata tcaagacaat ttagatttta
    41761 tcgattattt atcacgcaaa aatgttcctt atcaatttga agatggacca ggagatcatg
    41821 attatgcata ttgggaccaa gcgattaagc gtgctataac atggatggtg aatgattaat
    41881 tatttcctgg aaaatatgtg cctacattaa atacacagag tgagtgatac aaactattta
    41941 cgcacgacta acatttctga gtgtttaaat taattttgta ttaatatgat tggtgcaatt
    42001 tgctgataca caaaaatgtt tctcgtgaaa cttagattta gcttatagtt ttatcatcat
    42061 ttgtatgact tacattataa attttattat aatgaggtta acgctttgaa aggagtcatc
    42121 atcatgtcga ccaataaaaa cgattatgag catatgttgt tttattttgc atataaaacg
    42181 tttattacta ccgctgatga aattatagag aagtatggta tgagccgtca gcatcatcgt
    42241 tttttgtttt ttatcaataa attacctggt attactatta aatcattact agaaatatta
    42301 gaaatttcta aacaaggatc acatgcaaca cttcaaaaat taaaagagca aggtctcatt
    42361 attgaaaaag ttttagagac tgatcgacgt gtcaaaaaat tatattcgac ggataaaggc
    42421 gatcaactca ttgctgaatt gaacaaggcg caagatgaat tattgcaaaa tatatatcaa
    42481 caagtcggtt cggattggta tgatgtgatg gaagcattgg ctaaagggcg acctggcttt
    42541 gattttatta agcatttgaa agatgaaaaa gaaagctagc atcagaaatg ttgaaaatct
    42601 tcgcattctt aaatttaaaa aatatgtcaa aaagtgtata ataaaaacat ataatttaat
    42661 tgaactcagt ttcaacacat cttagaaagg agtttgaatg atgaaaaaat tagcagttat
    42721 tttaacatta gttggcggtt tatacttcgc atttaaaaaa taccaagaac gtgttaacca
    42781 agcacctaac attgagtact aaattaaacc ataaaaaatt cccgaacatc ttgttatagt
    42841 gctcgggaat ttttttatgc tttacttgaa tatatcaaat attatttttg cgctttctgt
    42901 attttcgata ttaccactaa atgattctga tctaggtcca taagcgtaag tattaacatc
    42961 ttcgcctgta tgtccatcgg aagtccatcc tgtataagat ttatcattta ctggcttctg
    43021 aattgcgtgt tgtaaggctt ttgtttgttt ttctacatct gcagattttt catctttttc
    43081 ttttttaagt agtcttttta gctttttatt ctctttttta acttttttca tatcatcttg
    43141 tgaaaattca aagccataac cttcattaat aactttttca gggtcttcac ctttagccat
    43201 tttttctgtc atatatgatc cagagtgttt catcgattta atcggttgag gattccattc
    43261 gtatccttta tctttaccaa ttgttagacc accagtagag tgatctgctg ttgcaacgac
    43321 aagtgtatct ttatgtttct tagcatattg aatggcgtca tcgaatgctt tttcaaaacc
    43381 ttccatttca gacatgactc ctgtaatatc gttagaatgc gctgatttat ctatagaagc
    43441 accttcgacc attaaaaaga aacctttttt attacgctca agcttactaa gtgcactttg
    43501 ttgcatatca gctaatgatg gttcgtcttt agaagcatct attgcaagtg gcatattttt
    43561 atctgcaaac aaaccaagca ctttatcttt atcagatttt gataactcct tactgttcgt
    43621 ggcaaggtcg taaccatctt ttttgaattt tttatctaaa ttgccattac ttttaccgaa
    43681 atatttagcg ccaccgccta ataaaacatc aactttatgc tttccgttga ttttatcttt
    43741 ataaaattgt ttagcgattt cgtttttatc atctctagaa gtcacgtgtg cagcatatgc
    43801 tgctggtgtt gcatctgtta attcagctgt tgaaacaaga ccagtcgact tacctttttc
    43861 ttttgcacgt tcaagcaccg tctttacttt ctgcttgtta ctgtcaacac cgatggcacc
    43921 attatatgtc ttatgaccag aactaaaggc tgttccgcca gctgcagaat cagtaatatt
    43981 ctgttttggg tcatttgaat atgtacgatt tgtgcctttt aaatatgaat caaaagcagt
    44041 aggagtcatt tctttagcat gaggatcatt tttataataa cgataagctg tgttaaatga
    44101 tggacccatg ccatcgccaa ctaaaaagat aacatttttt ggatttttag tattaccaac
    44161 cgcgaaactt tcatctttag aacttttatc ggattgcgca attgcaggtg tgacagagct
    44221 aaaaactgtt gacacgataa taaggttagc aactgcaaat tttgtggctt ttttaactga
    44281 taacataaga catcctcctg agtatatgac tatgtcttca gtgtaaaaga ggaattttga
    44341 gcaattatgt agttttagtt aaaaatatgt aaacagagtg atttagaata acaaaaaatg
    44401 aatatatatg acaatttgtt atagaaagcg ttagaataga agcgtgtgaa aatatagaat
    44461 taaatataat ttgaggtgga aaaatgatac tagtaatgtt atctccatta ttaatcatat
    44521 tctttatagt gttgtctatt ttagaagagc gtaaacgtac gaagaaaaag caactcgaga
    44581 aagaaaaagc aaatacacta aatcaaaata caaatgacac ggaaagttca aatcaagagc
    44641 cgtcattgca gcaggctaaa gaacaaaaag ataacaaagg ataattcaat tgaaggaaga
    44701 agattataga tgaaaatatt aattgttgaa gatgattttg ttatagcaga aagtttagca
    44761 tctgaactta aaaaatggaa ttacggtgtt attgtcgttg aacaatttga tgatatactg
    44821 tctatcttta accaacatca acctcagctt gtattgctag atattaattt gccaacgtta
    44881 aatggttttc attggtgtca agaaatccga aaaacatcta atgtgccaat tatatttatt
    44941 agttcccgta ttgataatat ggaccaaatt atggcaatac aaatgggggg agatgatttt
    45001 atcgaaaagc catttaactt gtcattaacg attgccaaaa ttcaagcatt attgagacga
    45061 acttatgact tgtcagtagc taatgattca ttgacggtta aaggttgtac attaatatta
    45121 gatgaagcaa aagtcgtgta tcaagaacaa agcatacagc tatctttgac tgaattacaa
    45181 atattaaagt tattatttca aaatgaagat aaatatgtaa gtagaactgc tttaattgaa
    45241 aaatgttggg aatcagaaaa cttcatagat gataacacat tagctgttaa catgacgcgc
    45301 ctgcgtaaaa aattaaatac tattggcgtt aatgatttta tcattacaaa gaaaaatgtc
    45361 ggatataaag tatagggtga atgcaatgac ctttcttaaa agtattactc aggaaatagc
    45421 aatagtcata gttatttttg ctttatttgg cttaatgttt tacctgtatc atttgccatt
    45481 agaagcatat ttactagcac ttggcgttat tttattatta ttactcatat tcataggtat
    45541 taaatattta agttttgtaa aaactataag ccaacaacaa caaattgaaa atttagaaaa
    45601 tgcgttgtat cagcttaaaa atgaacaaat tgaatataaa aatgatgtag agagctactt
    45661 tttaacatgg gtacatcaaa tgaaaacacc cattactgca gcacaactgt tacttgaaag
    45721 agatgagcct aatgttgtga atcgtgttcg tcaagaggtt attcaaattg ataactatac
    45781 aagtttagca cttagttatt taaagttatt aaatgaaact tctgatattt ctgtcactaa
    45841 aatttcgatt aacaatatca ttcgcccaat tattatgaaa tattcaatac agtttattga
    45901 tcaaaaaaca aaaatccatt atgaaccttg tcatcacgaa gtattaactg acgttagatg
    45961 gacctcttta atgatagaac aattaataaa taatgcactt aagtatgcga gaggtaaaga
    46021 tatatggatt gaatttgatg agcaattcaa tcaattatac gtaaaagata atggtatcgg
    46081 tattagtgaa gcggacttgc ctaaaatatt tgataagggc tattcaggtt ataatggcca
    46141 gcggcaaagt aactcaagtg ggattggttt atttatcgta aaacaaattt caactcacac
    46201 aaaccatcct gtttcagtcg tatctaaaca aaatgagggt acaacattta cgattcaatt
    46261 tccagatgaa taaaaacttt caatattgta agcatactag taacattttt tcactaattt
    46321 aaatgttatt agtatttttt tgttttaata tagaactaac aaagaaatga ggtgcatgcc
    46381 atgttgctag aagtgaaaca tgtaaaaaag gtttatggta aaggtttgaa tgctacgaca
    46441 gcacttaatc aaatgaattt atcagttgga gctggtgaat ttgttgcaat tatgggtgag
    46501 tctgggtcag ggaagtctac actactaaat ttaattgctt cttttgatgg attaactgaa
    46561 ggtgacatta ttgtggatgg cgcacattta aataatatga aaaataaaag taaagcattg
    46621 tatcgtcaac aaatggtagg ttttgttttt caagatttta atcttttacc aacaatgacg
    46681 aataaagaaa atataatgat gccattaatt ttagctggtg ctaaacgaaa agatatagaa
    46741 caaagggtac atcagttgac agtacaatta catttagagg gattcttaaa caaatatcct
    46801 tctgaaatct cagggggtca gaagcaacgt attgccattg cacgtgcatt agttactaaa
    46861 ccgacgattt tactagccga tgaacctaca ggtgcactgg attcaaaaac atcaaaagca
    46921 ttgatgaagt tatttcaaga gattcatcaa ttggaacaga caattttaat ggtaactcat
    46981 tcaaatatcg atgcgtctta tgcagagcga gtcattttta ttaaagatgg gcgtctatat
    47041 catgaaatat atcgtggtga agaaagtcaa ttagtttttc aacagcgaat aacagatagc
    47101 ttagcacttg tgaatggagg aagtgtcaat atatgaagtt aagattgtta tggcacatag
    47161 tacgacgtca atttattacg cagcgacttg taatcattcc attcatttta gcggtaagtg
    47221 tactattcat gattgaatat acgcttgtat caattgggtt aaatagctac ataaaacaga
    47281 agaatgactt cctagtacca tttattatca tagctaattt ttttatggcg cttttaactt
    47341 ttatttttat tttctatgca aatcacttta tgatgtcaca aagacgaaaa gagtttagca
    47401 tttttatgac attgggcatg accaagaaaa gtatgcgttt aattgtagtg atggaaacta
    47461 tcttacaatt tatgataatt tcagtcatta gtattgccgg cggatactta cttggtgcga
    47521 tatttttctt gtttatacag aaaataatgg gcagtgaagt tgctacgtta aggtattatc
    47581 catttgatac tgtagcgatg tttattactt tgattatcat tgctgtatta atgggcatgc
    47641 tacttatatt caacttgttc agtattaatt ttcaacgacc gataacttat caacatcgtt
    47701 ccgattccag tgtcatatca cgatggttgc gttacgtttt aattgttata ggaagcgcag
    47761 cactatattt aggctatttt attgcattac aacaagatac gacgtttggt gcttttttta
    47821 aaatatggat tgtcatagga ttagttatta tcggtactta tgcatttttt gtaggtataa
    47881 gtgaaataat tattagtata ttgcagcagg tatcaaaagt ttactatcat ccacggtatt
    47941 tttttgtggt agttgggatg cgtgtacgtc ttaaaatgaa tgcagtcagt cttgcaacaa
    48001 tcactttgct gtgtacattt ttgattgtaa cgctcacaat gacactaaca acctatcgcg
    48061 atatgaatca taccattatg aagttgatta ccaatgatta tgatttgtca tttaacgaca
    48121 attctaagtc acaaatagaa cgtcaacaaa cgattgagca tattaaacgt gatatacagg
    48181 gttcggttaa tgcaaaagac ttcaaagtat atgaaacgac attatttaga gcttctttag
    48241 aaaagaatcg tgcttattac aagttaaaac aggctagtga tgatattcca atagacttca
    48301 ttggtaatga aggctcgata ttttctaagc aatcagtaat gatcacagca cttactgtgc
    48361 aagattataa tcgatatcac caaaacaaat tacacttaga caatcaaagt ataggtatga
    48421 ttacgaatgt acctattttt aaaaagcagt ctaaggtcgg tcttaataat gaagtcttta
    48481 aagtgaaaca acttgatgca catgcgttaa atttgatgat gattcaagat agtatggttt
    48541 taattgttaa agataacaat caattacaaa aagtaaaagg attttatgca catgaacaaa
    48601 agaagtcgac aatatcgata aactttaatg tatctggaaa gacgaaatta acaacatcac
    48661 aaattcaaaa gctttcgaaa aagtacgatg catcaataaa ttctaaatca gaaatgctaa
    48721 tggtttggga tcgtctgaca ggtggactaa tatttgtagg tggcgtggta tcaattgtac
    48781 ttgtcattgg aatatttcta atgatgtact ataagcaagt ttcagaagcc tatgcaaatc
    48841 agcacaatta tgacattatg aaaaaacttg gtttagataa tgggagaata tcaaagataa
    48901 cacgaaatca aatgactttc ttgttcgcaa taccaataac agtagcactg attcatacat
    48961 taatttctag caacattgtt tatacgttac taaatatgct agggattaat aaccatcata
    49021 tatttttaac atgttacgta ctagctgtca ttataatttc gtttctatat atggcgatgt
    49081 acaaaataac ttcctacatt tatgctaaag ttattcatca acaacgcaat taaccatgtt
    49141 tcagtgaaat cacaatcaat catgtgtttt cactgaaata tattatatga aaaagaaaac
    49201 ctaaaaatac tacaaataac aataacaatt ttcaaccgat aagataaaat gtcttatcgg
    49261 ttttttactt gtatgacaag ttatattttt gaaatatcaa aaatatatag cttgtttata
    49321 taaaatgatt caaaatgttt tataacaaca tattttatgc aacacataaa atgggtaact
    49381 aacatcacga aatcattgta tttgtagtta cactgatgaa gcgatgtcac aaagaaatgt
    49441 cgttatcaaa cgaagcccga aatacatact gatactcgaa gtaatgtaac gataaataac
    49501 aatgataacg attgattact acgcaaaaat atatgtaaat tgacgattta aatgcaattc
    49561 gtatatattt tccaatgatt tacacattaa aatgtttgaa aattgtcaca attcagtcta
    49621 attttaaagg aaattaaaat taaattaatt acaaaattca
//
LOCUS       DATTQF010000006        48870 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.006, whole genome shotgun sequence.
ACCESSION   DATTQF010000006 DATTQF010000000
VERSION     DATTQF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 48870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48870
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.006"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(49..1431)
                     /locus_tag="VB241_000249"
     CDS             complement(49..1431)
                     /locus_tag="VB241_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP1243582.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            1535..2422
                     /gene="pdxS"
                     /locus_tag="VB241_000250"
     CDS             1535..2422
                     /gene="pdxS"
                     /locus_tag="VB241_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEP1243583.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            2426..2986
                     /gene="pdxT"
                     /locus_tag="VB241_000251"
     CDS             2426..2986
                     /gene="pdxT"
                     /locus_tag="VB241_000251"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEP1243584.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3193..4407)
                     /locus_tag="VB241_000252"
     CDS             complement(3193..4407)
                     /locus_tag="VB241_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP1243585.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            4565..5026
                     /locus_tag="VB241_000253"
     CDS             4565..5026
                     /locus_tag="VB241_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP1243586.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5045..5611
                     /locus_tag="VB241_000254"
     CDS             5045..5611
                     /locus_tag="VB241_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEP1243587.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLVE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            5601..6608
                     /locus_tag="VB241_000255"
     CDS             5601..6608
                     /locus_tag="VB241_000255"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEP1243588.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            6622..9078
                     /locus_tag="VB241_000256"
     CDS             6622..9078
                     /locus_tag="VB241_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP1243589.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            9562..10926
                     /gene="radA"
                     /locus_tag="VB241_000257"
     CDS             9562..10926
                     /gene="radA"
                     /locus_tag="VB241_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP1243590.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELVGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            10951..12024
                     /locus_tag="VB241_000258"
     CDS             10951..12024
                     /locus_tag="VB241_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEP1243591.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIIGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            12581..14035
                     /locus_tag="VB241_000259"
     CDS             12581..14035
                     /locus_tag="VB241_000259"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP1243592.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMEISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            14459..15106
                     /gene="cysE"
                     /locus_tag="VB241_000260"
     CDS             14459..15106
                     /gene="cysE"
                     /locus_tag="VB241_000260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP1243593.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15090..16490
                     /locus_tag="VB241_000261"
     CDS             15090..16490
                     /locus_tag="VB241_000261"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP1243594.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQTVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGA
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKKADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            16483..16887
                     /locus_tag="VB241_000262"
     CDS             16483..16887
                     /locus_tag="VB241_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP1243595.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNEIITIVNER"
     gene            16895..17641
                     /gene="rlmB"
                     /locus_tag="VB241_000263"
     CDS             16895..17641
                     /gene="rlmB"
                     /locus_tag="VB241_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP1243596.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            17641..18165
                     /locus_tag="VB241_000264"
     CDS             17641..18165
                     /locus_tag="VB241_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP1243597.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDTIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18246..18815
                     /locus_tag="VB241_000265"
     CDS             18246..18815
                     /locus_tag="VB241_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEP1243598.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSAEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            18930..19073
                     /gene="rpmG"
                     /locus_tag="VB241_000266"
     CDS             18930..19073
                     /gene="rpmG"
                     /locus_tag="VB241_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1243599.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGTATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19129..19311
                     /gene="secE"
                     /locus_tag="VB241_000267"
     CDS             19129..19311
                     /gene="secE"
                     /locus_tag="VB241_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP1243600.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19324..19872
                     /gene="nusG"
                     /locus_tag="VB241_000268"
     CDS             19324..19872
                     /gene="nusG"
                     /locus_tag="VB241_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP1243601.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            20053..20475
                     /gene="rplK"
                     /locus_tag="VB241_000269"
     CDS             20053..20475
                     /gene="rplK"
                     /locus_tag="VB241_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP1243602.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            20683..21372
                     /gene="rplA"
                     /locus_tag="VB241_000270"
     CDS             20683..21372
                     /gene="rplA"
                     /locus_tag="VB241_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP1243603.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASL"
     misc_feature    21463..21609
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            21647..22147
                     /locus_tag="VB241_000271"
     CDS             21647..22147
                     /locus_tag="VB241_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP1243604.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            22190..22558
                     /gene="rplL"
                     /locus_tag="VB241_000272"
     CDS             22190..22558
                     /gene="rplL"
                     /locus_tag="VB241_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP1243605.1"
                     /translation="MANHEQIIETIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            22733..23341
                     /locus_tag="VB241_000273"
     CDS             22733..23341
                     /locus_tag="VB241_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1243606.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKINLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            23556..27107
                     /gene="rpoB"
                     /locus_tag="VB241_000274"
     CDS             23556..27107
                     /gene="rpoB"
                     /locus_tag="VB241_000274"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP1243607.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEFNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            27271..30867
                     /gene="rpoC"
                     /locus_tag="VB241_000275"
     CDS             27271..30867
                     /gene="rpoC"
                     /locus_tag="VB241_000275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP1243608.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFNITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEVI
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            31004..31258
                     /locus_tag="VB241_000276"
     CDS             31004..31258
                     /locus_tag="VB241_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEP1243609.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            31356..31769
                     /gene="rpsL"
                     /locus_tag="VB241_000277"
     CDS             31356..31769
                     /gene="rpsL"
                     /locus_tag="VB241_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP1243610.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            31835..32305
                     /gene="rpsG"
                     /locus_tag="VB241_000278"
     CDS             31835..32305
                     /gene="rpsG"
                     /locus_tag="VB241_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP1243611.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            32428..34509
                     /gene="fusA"
                     /locus_tag="VB241_000279"
     CDS             32428..34509
                     /gene="fusA"
                     /locus_tag="VB241_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP1243612.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIANADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            34726..35910
                     /gene="tuf"
                     /locus_tag="VB241_000280"
     CDS             34726..35910
                     /gene="tuf"
                     /locus_tag="VB241_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP1243613.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(36191..37366)
                     /locus_tag="VB241_000281"
     CDS             complement(36191..37366)
                     /locus_tag="VB241_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1243614.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSKIQEHVMHKMDKL
                     LQGLAIANDIEYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            37537..38724
                     /locus_tag="VB241_000282"
     CDS             37537..38724
                     /locus_tag="VB241_000282"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEP1243615.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMASTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGEEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     NLI"
     gene            39000..39878
                     /gene="hchA"
                     /locus_tag="VB241_000283"
     CDS             39000..39878
                     /gene="hchA"
                     /locus_tag="VB241_000283"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HEP1243616.1"
                     /translation="MSQDVNKLSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPAKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALENDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQSLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            40036..41673
                     /locus_tag="VB241_000284"
     CDS             40036..41673
                     /locus_tag="VB241_000284"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEP1243617.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIEGELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KVDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKDTMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYTNVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            41887..42852
                     /locus_tag="VB241_000285"
     CDS             41887..42852
                     /locus_tag="VB241_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEP1243618.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFTRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IDAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPDFKLEYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLVSMTKLMLEAIEQKDTVKNNN"
     gene            43187..44263
                     /locus_tag="VB241_000286"
     CDS             43187..44263
                     /locus_tag="VB241_000286"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP1243619.1"
                     /translation="MSQAVKIELRETLKEKPDTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKKDGEVALFRPDENFKRLNNSLARLE
                     MPQVNEGELLEGLKQLVDLEREWVPEGEGQSLYIRPFVFATEGVLGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            44514..45197
                     /locus_tag="VB241_000287"
     CDS             44514..45197
                     /locus_tag="VB241_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1243620.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALI"
     gene            complement(45313..45975)
                     /locus_tag="VB241_000288"
     CDS             complement(45313..45975)
                     /locus_tag="VB241_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP1243621.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(45968..46585)
                     /locus_tag="VB241_000289"
     CDS             complement(45968..46585)
                     /locus_tag="VB241_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP1243622.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            46652..47122
                     /locus_tag="VB241_000290"
     CDS             46652..47122
                     /locus_tag="VB241_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEP1243623.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEDCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            47269..48138
                     /locus_tag="VB241_000291"
     CDS             47269..48138
                     /locus_tag="VB241_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1243624.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDVSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            48158..48724
                     /locus_tag="VB241_000292"
     CDS             48158..48724
                     /locus_tag="VB241_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1243625.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT        17540 a         7115 c         9416 g        14799 t
ORIGIN      
        1 aatagacaag ctattgcata agtaacacta acttttgtca aagaagtgtt actttataat
       61 taatgatttt attagagcgt ctacatgcgg ttttaaagca tcatcgtcta taccgccaaa
      121 gcctaatata aatttagggg ttttcttata gtcttggtca tcatcaaaat tataaacttg
      181 taattttaac tttactttat ttgctctatc aagacactct tgtaatgtta atccattttt
      241 tactgtaatt gtaaaatgca tacctgtttc agcaccttga atatcaagtt gctctttgta
      301 tggttttaat ctttttaaaa tataggttag ttttctacga taaattcgtc tcattttatt
      361 taaatgcctt tcaaaaccac cggaagatat aaacgttgca ataaggtttt gcatatgaac
      421 aggtacagtg ttgccttcaa tgtgattttg agaatgatat tttttcatta tagaataggg
      481 taacaccata tatgcaacac gacagctagg aaaaatagac tttgaaaatg tactgatata
      541 aatcactttt tctcctcttg aatatagacc ttgaattgct ggaataggtt taccgaaata
      601 tctaaactcg gaatcataat catcttctat aataaatcgt tcttcttttt cttgagccca
      661 ttgtattaat tgagttcgtt tttttaaatc cattacatat ccagttggaa attgatggga
      721 aggcgttata tatactatat ttttttgtga tttaataact tcatctacgt ttattccatt
      781 atcttcaact tcaatttgtt catattcaac ttgtttttta tctaaaatat ttttgattgg
      841 tggataacta ggtttttcga taataaacgt tgaagtataa agtaaatcga ctaattgatt
      901 tactaattgt tcggtagatg aaccaattat aatttgatta ggatcacaaa ttacgccacg
      961 attagtaaat aaataaaatg ccagttgaaa ccgcaaatgt aattctcctt gaaagtgtcc
     1021 tctacgtaat tgatttaaat gatttgtatc ataaagatct ttggaatact ttctgaaaag
     1081 ttctataggg aaatgtttcg tatctatttc atccaaatta aaagcataat cataagcttc
     1141 atcactcgct tttggtttat atgaatcatc atcaaaaaga gaagggatag gttgattgtt
     1201 taaaattgtt aaagattcaa tttcggacac aaaatatcca gagcgaggtc ttgaataaat
     1261 gtaaccttca tctaatagaa gttgatatgc atgttctacg gttgtttggc tgatagataa
     1321 atgtttgctt aattgtcttt tagaataaaa tttatcacct tctttaaatt gaccttcaat
     1381 tatttgtttt tttaattttt cataaagttg atggtataaa gtgtttttca attttataac
     1441 tgacctccta aatttgtctt attttgtacc tttttaaata tcagtttata cattacaatg
     1501 tatttaatca acttgaaaag gggttttatg tataatgagt aaaattattg gatcagacag
     1561 agtcaaaaga ggtatggctg aaatgcaaaa aggcggcgtt attatggatg tcgttaatgc
     1621 tgagcaagca agaattgcag aagaagctgg cgcggtagca gttatggcat tagaacgagt
     1681 accttctgat attagagctg ctggtggcgt tgcacgtatg gcaaacccta aaattgtaga
     1741 agaagtaatg aatgctgttt ctattccagt catggctaaa gcacgtattg gtcatatcac
     1801 tgaagcaaga gtattagagg cgatgggtgt tgactatatt gatgaatcag aagtgttaac
     1861 accagcagat gaggaatatc acttaagaaa agatcaattt acagtaccat ttgtatgtgg
     1921 atgtcgtaat ttaggtgaag ctgcgcgtag aattggtgaa ggtgctgcta tgttacgtac
     1981 taaaggtgaa ccaggtacag gtaatattgt tgaagctgta agacatatga gacaagttaa
     2041 ttcagaagtt agtcgattga ctgtaatgaa tgatgatgag attatgactt tcgcgaaaga
     2101 tatcggtgcg ccttatgaaa ttttaaaaca aattaaagac aatggtcgtt taccggtagt
     2161 taactttgca gctggtggcg ttgcgactcc tcaagatgct gctttaatga tggaattagg
     2221 tgccgatggt gtattcgttg gatcaggtat ttttaaatca gaagatccag aaaaatttgc
     2281 taaagcaatt gttcaagcaa caacacatta ccaagactat gaactaattg gaagattagc
     2341 aagtgaactt ggcactgcta tgaaaggttt agatatcaat caattatcat tagaagaacg
     2401 tatgcaagag cgtggttggt aagatatgaa aataggtgta ttagcattac aaggtgcagt
     2461 acgtgaacat attagacata ttgaattaag tggtcatgaa ggtattgcag ttaaaaaagt
     2521 tgaacaatta gaagaaatcg agggcttaat attacctggt ggcgagtcta caacgttacg
     2581 tcgattaatg aatttatatg gatttaaaga ggctttacaa aattcaactt tacctatgtt
     2641 tggtacatgc gcaggattaa tagttctagc gcaagatata gttggtgaag aaggatacct
     2701 taacaagttg aatattactg tacaacgaaa ctcattcggt agacaagttg acagctttga
     2761 aacagaatta gatattaaag gtatcgctac agatattgaa ggtgtcttta taagagcgcc
     2821 acatattgaa aaagtaggtc aaggcgtaga tatcctatgt aaggttaatg agaaaattgt
     2881 agctgtccag caaggtaaat atttaggcgt atcattccat cctgaattaa cagatgacta
     2941 tagagtaact gattacttta ttaatcatat tgtaaaaaaa gcatagctta atgtatgcta
     3001 aatcaacgaa ttattgatat ttatagattt gttgagaaga aaattactcc ttcaaactta
     3061 gctttggagg agttattttt tatgtcaaaa ttaaaaatga taaaagataa agatatacat
     3121 aagaaaaaac ccttcaaaga gactgagaat agtcaaaatt ttgaaggggt taattcgatg
     3181 ttgatgtatt tgttaaataa agaatccagc gattgcagct gaaatgaaag atactagtgt
     3241 tgcaccgaat aataatttca aaccaaagcg ggcaactgta tctccttttt tgtcattaag
     3301 tgatttaatc gcacctgaaa taataccaat agagctaaag ttagcaaatg atactaagaa
     3361 tacagatgta acaccttttg cgtgttcaga taaatcacta agtttaccaa gtgcttgcat
     3421 tgctacaaat tcgttagata atagttttgt tgccataact gaaccggctt gaactgcatc
     3481 ttgccatggc acacctacta agaatgcaaa tggtgcaaag acaaaaccaa ttaatgtttg
     3541 gaaatcccaa gaaatagcgc cacctgaaac tgtactaaag atattgctta caattccatt
     3601 taatagagcg ataatggcaa tgtaaccgat tagcattgcg cctacaatga cagctacttt
     3661 aaatccatct aaaatatatt ctcctagcat ttcaaagaat gattgttgtc tttcttcagt
     3721 ttcttcaact aataatttgt catcttcttc attaacttta taagggttaa taattgaagc
     3781 gatgataaaa ccaccaaata agtttaagac aacagccgtt acaacatatt taggttcaat
     3841 taaggtaaag tatgcaccga taattgaagc agaaacagtc gacattgctg aagctgttaa
     3901 tgtgtataaa cgttgcttag gtatgtatgg taattgtttt ttaattgaaa taaatacttc
     3961 agattgtccc aaaattgctg cagcaactgc attgtatgat tctaaacgtc ccataccatt
     4021 aattttagaa attaagaatc ctaaaacatt aatgattaaa ggtaaaatct ttgtgtattg
     4081 aaggataccg ataatcgctg aaataaatac gataggtaat aatacactga agaagaatgg
     4141 tggttgctta ggatcgatat attgaatacc accgaataca aagttaacac catctgcagc
     4201 ttttaataat aagtagttaa aaccgttcga aataccacca ataaccttga ttcccattgt
     4261 agttttaagc aagataaatg caaagataag ctgaattgca agtaaaattc ctacatattt
     4321 ccagcgaata tttttcctgt ctgagctaaa tagaaacgca agtgctaaaa agaagataat
     4381 tccgataatc ccaattagaa tatgcatata tttctcattc ctttagtttt ttctacaatc
     4441 tatcatacaa taaaatggaa gggctaacat cataaatttt tgaaaatata aaaacaaatt
     4501 aattgaaaaa ggtcaaaata ggtcatataa tatagtcaaa gaaggtcaaa aaggggtgat
     4561 atacatgcac aatatgtctg acatcataga acaatacatc aaacgtttat ttgaagagtc
     4621 gaatgaggat gtcgttgaaa ttcagagagc gaatatcgca cagcgttttg attgcgtacc
     4681 atcacagtta aactatgtaa tcaaaacacg attcactaat gaacatggtt atgaaatcga
     4741 aagtaaacgt ggtggtggtg gttacatccg aatcactaaa attgaaaata aagatgcaac
     4801 aggttatatt aatcatttgc ttcagctgat tggaccttct atttctcaac aacaagctta
     4861 ttatattatt gatgggcttt tagataaaat gttaataaat gaacgtgaag ctaaaatgat
     4921 tcaagcagtt attgatagag aaacgctatc aatggatatg gtttctagag atattattag
     4981 agcaaatatt ttaaaacgtt tgttaccagt tataaattat tactaaatga aatgaggtgt
     5041 tgaagtgctt tgtgaaaatt gtcaacttaa tgaagcggaa ttaaaagtta aagttacaag
     5101 taaaaataaa acagaagaaa aaatggtgtg tcaaacttgt gctgaggggc accatccgtg
     5161 gaatcaagct aatgaacaac ctgaatatca agaacatcaa gataatttcg aagaagcatt
     5221 tgttgttaag caaattttac aacatttagc tacgaaacat ggcatcaatt ttcaagaagt
     5281 agcgtttaaa gaagaaaaac gttgcccatc atgtcacatg actttgaaag atattgcaca
     5341 tgttggtaaa tttgggtgtg ctaattgtta tgcaacattt aaagatgaca tcattgatat
     5401 cgtccgcaga gttcaaggtg gacaatttga gcacgttgga aagacaccac attcttcaca
     5461 taaaaagata gctttaaagc gaaaaatcga agaaaagaat gaatatttga aaaaacttgt
     5521 tgaaatccaa gattttgagg aagcagccat tgttagagat gaaattaaag cactaaaagc
     5581 tgagagtgag gtgcaacatg atgacgcata atattcatga taatatcagc caatggatga
     5641 aaagtaatga agaaacacca attgttatgt cttctagaat tcggttagcg cgtaatttag
     5701 aaaatcatgt gcatccacta atgtatgcta ctgaaaatga tggatttaga gttataaatg
     5761 aggtacaaga tgccttgcca aattttgaat taatgcgtct tgatcaaatg gatcaacaaa
     5821 gtaaaatgaa aatggttgca aagcatttga ttagccctga actaataaaa caaccagcag
     5881 ccgcagtatt agtgaatgat gatgaatctt taagtgtcat gataaatgaa gaggaccata
     5941 ttcgtattca agctatggga actgacacga cattacaggc tttatataat caagcttcat
     6001 caattgatga tgaattagat cgaagccttg atataagtta tgatgaacaa cttggttatt
     6061 taactacatg tcctaccaat ataggtactg gtatgagagc aagcgtgatg ctacatttac
     6121 caggtctatc tattatgaaa agaatgacac ggattgctca aaccattaat cgttttggat
     6181 atacaatcag aggtatttac ggtgaaggtt cgcaagttta tggacatact tatcaagtat
     6241 ccaaccaact tacacttggt aaatctgagt tagaaatcat agaaacatta acagaagttg
     6301 ttaatcaaat cattcatgaa gaaaaacaaa tacgacaaaa gttagacact tataatcaac
     6361 tagaaacaca agaccgtgtt tttcgctcgc taggtatttt acaaaactgt agaatgataa
     6421 ctatggaaga ggcttcttat agattaagcg aagttaaact tggtatagat ttaaattaca
     6481 ttgaattaca aaactttaaa tttaatgaat tgatggtagc tatacagtca ccatttttat
     6541 tagatgaaga agatgacaaa tctgtaaaag aaaaacgagc agatatacta agagaacata
     6601 taaagtagga ggtcattatt tatgttattt ggtagattaa ctgaacgtgc acagcgcgta
     6661 ttagcacatg cacaagaaga agcaattcgt ttaaatcatt caaatatagg aacagaacac
     6721 ctattattgg ggttaatgaa agaacctgaa ggaattgctg caaaagtatt agaaagtttt
     6781 aatatcactg aagataaagt aattgaagaa gttgaaaaat taatcggaca tggtcaagat
     6841 catgttggta cattgcatta tacacctaga gctaaaaaag tcattgaatt atcgatggat
     6901 gaagctagaa aattacatca caattttgtt ggaacggaac atattttatt aggcttgatt
     6961 cgtgagaatg aaggtgttgc agcaagagtt tttgcaaatc tagatttaaa tattactaaa
     7021 gcacgtgcac aagttgtgaa agctttagga aaccctgaaa tgagtaataa aaatgcacaa
     7081 gctagtaagt caaataatac tccaacttta gatagtttag ctcgtgactt aacagtcatt
     7141 gccaaagacg gtacattaga tcctgttata ggacgtgata aagaaattac acgtgtaatt
     7201 gaagtattaa gtagacgtac gaaaaacaat cctgtgctta ttggagagcc aggtgttggt
     7261 aaaactgcta ttgctgaagg tttagcgcaa gccatagtga ataatgaggt accagagaca
     7321 ttaaaagata agcgtgttat gtctttagat atgggaacag tagttgcagg tactaaatat
     7381 cgtggtgaat ttgaagagcg tctgaaaaag gttatggaag aaatccaaca agcaggtaat
     7441 gtcatcctat ttattgatga attgcatact ttagttggtg ctggtggtgc tgaaggtgct
     7501 atcgatgctt cgaatatttt gaaaccggca ttagcacgtg gtgaattaca atgtattggt
     7561 gctactacat tagatgaata tcgcaaaaat attgaaaaag acgcggcttt agaacgtcgt
     7621 ttccaacctg tacaagttga tgaaccttca gtagtagata cagttgctat tttaaaagga
     7681 ttaagagatc gttacgaagc acaccatcgt attaatattt cagacgaagc tattgaagca
     7741 gctgttaaat taagtaacag atatgtttca gatcgtttct taccagataa agcaattgat
     7801 ttaattgatg aagcaagttc taaagtaaga cttaagagtc atacgacacc taataattta
     7861 aaagaaattg aacaagaaat tgaaaaagtt aaaaatgaaa aagatgccgc agtacatgct
     7921 caagagtttg aaaatgctgc taatctgcgt gataaacaaa caaaacttga aaagcaatat
     7981 gaagaagcta aaaatgaatg gaagaacgca caaaatggca tgtcaacttc attgtcagaa
     8041 gaagatattg ctgaagtcat tgcaggatgg acaggtatcc cattaactaa aatcaacgaa
     8101 acagaatctg aaaaacttct tagtctagaa gatacattac atgagagagt tattgggcaa
     8161 aaagatgctg ttaattcaat cagtaaagcg gttagacgtg cccgtgcagg gttaaaagat
     8221 cctaaacgac caattggtag ctttatcttc cttggaccaa ctggtgttgg taaaactgaa
     8281 ttagctagag ctttagctga atcaatgttt ggcgatgatg atgcgatgat ccgtgtagac
     8341 atgagtgaat ttatggaaaa acacgcagtg agccgattag ttggtgctcc tccaggatat
     8401 gttggtcatg atgatggtgg acaattaact gaaaaagtta gacgtaaacc atattctgta
     8461 attttatttg atgaaattga aaaagcacat ccagatgtat ttaatattct attacaagtt
     8521 ttagatgacg gacatttgac agatacaaaa ggacgtacag ttgatttcag aaatacaatt
     8581 atcataatga catcaaacgt tggagcacaa gaattacaag atcaacgatt tgctggattc
     8641 ggtggttcaa gtgatggaca agattatgaa acaattcgaa aaacgatgtt aaaagaatta
     8701 aaaaattcat tccgtccaga atttttaaac cgtgtagatg atatcattgt attccataaa
     8761 ctaacaaaag aagaattaaa agaaattgta actatgatgg ttaataaatt aacaaatcga
     8821 ttatccgaac aaaacataaa tattattgtt actgataaag cgaaagataa aatcgcagaa
     8881 gaaggatatg atccagaata tggtgcaaga ccattaatta gagcgataca aaaaactatc
     8941 gaagataatt taagtgaatt aatattagat ggtaatcaaa ttgaaggtaa gaaagttaca
     9001 gtagatcatg atggtaaaga gtttaaatat gacattgctg aacaaacttc agaaactaaa
     9061 acaccatcgc aagcataatt ataaaacagt ccaaaacaaa ttaaagtttt gggctgtttt
     9121 ttagtagcat tgaactatag aaattcgtga aagtatccac caacgaaaca acctaataaa
     9181 ataatcatca aaggatagtt aagaattata tgaaacaagt taatgagcat acagcgatag
     9241 taaaaaggta tgaattttta taaaggtttt ttgtttgaag atactagcaa cttacgtcaa
     9301 aataaaatag gtctatataa tattgtgata aatctagaga aaagagttta ctaagaaatt
     9361 attaagattt tatctttgaa agatacctac tatcaacatg atgtaagttt attttataat
     9421 attcataaaa aataaatatg ataaaacagt ttgcgttagt agtcatgtta aaatacaaac
     9481 aaatgtaact cacatttaat ttgtcataat gggaatgtgc gtttaaaata gatttgttca
     9541 aaggaaagtg gaggtgcaat tttggccaag aaaaaagtga tttttgaatg tatggcttgt
     9601 ggttatcaat ctcctaaatg gatggggaaa tgtcctaatt gtggcgcttg gaatcaaatg
     9661 gaggaaattg ttgaaaaagc agccaatcct aaacatggag tgaaaaccaa ggaattagta
     9721 ggtaaagtac aaaaattaaa tagtattaaa catgaaacaa cgccgagagt gttaacagat
     9781 tcagcagaat ttaaccgtgt attaggtgga ggtattgtga gcggatcgtt agtacttatt
     9841 ggtggggatc caggtattgg taagtcaacg ttacttttac aaatttgtgc atcgttatct
     9901 caaaagaaaa aagtactata tattactgga gaagaatcgc ttagtcagac taaattacgt
     9961 gcagagcgat tagatgaaga ttcaagtgaa ttgcaagtat tagctgaaac agatcttgaa
    10021 gttatttatc aaacagtaaa agaggaacaa cctgatttac tagtagtgga ttcgattcaa
    10081 acaatatatc atcctgaaat cagctctgcg ccaggttctg tttcacaagt tcgtgaaagt
    10141 acacaaagtt taatgaatat tgctaaacaa atgaacattg caacttttat agtgggtcat
    10201 gtaacgaaag aaggtcaaat tgctggccca agattgctag aacacatggt tgatactgta
    10261 ctttattttg aaggcgatga acaccacgca tatcgaattt tgcgagctgt taaaaaccgt
    10321 tttggttcaa cgaatgaaat gggaatcttc gaaatgaagc aaagtggatt aaaaggtgta
    10381 aataatccat ctgaaatgtt tttagaagaa cgttcaacaa atgttccagg ttcaacaatt
    10441 gttgcaacca tggagggaac tagaccactt ttaatagaag ttcaagcgct ggtaactcca
    10501 acgactttta acaatccgag acgaatggca acagggattg atcacaatcg attaagtttg
    10561 ttgatggctg ttttggaaaa gaaagaaaat tatctattac aacaacaaga tgcttatatc
    10621 aaagtagctg gcggtgtaaa gttaacggag ccagcagttg atttaagtgt aattgtagca
    10681 actgcgtcta gctttaaaga taaagctgtc gacggattag attgctatat tggagaagtt
    10741 ggtttaacag gtgaggtacg tcgtgtatct cggatagaac aacgcgtgca agaggctgca
    10801 aaactaggtt tcaaacgtgt aattattcct aaaaataata taggcggatg gacatatcct
    10861 gaaggtatac aagtaatagg tgtaactact gtacatgaag cattgtcgtt tgctcttcat
    10921 tcataaaaca tcaagaaagg aggacattgt gtgaatatcg ttaaactaat ggttattatt
    10981 atttacttaa ttattgggag cgcattagga ataattatta ttcctgaaat tgcaaatgat
    11041 cttggattac aaaactccag ctttttaaaa aatcactatg tagatggcat tatcggtagt
    11101 atttttatgt tcttaatttt tggtgtattt attagacgag ttactaacgc tataataggt
    11161 ttagaacatt ttattatgcg tagaagtgct gttgaaatac tattcgcaac aataggttta
    11221 ataatcggat tacttatttc tgttatggtg tcgtttatat tggaatcaat tggcaactct
    11281 atttttaatc atttcattcc tgtcataatt acgatattac tatgttattt cggtttccaa
    11341 tttggcctta aaaaacgaga tgaaatgtta atgtttttac ctgagaatat agcgcgttcc
    11401 atgtcacaac atactaaaag tgctacacca aaaattatcg acacaagcgc aattattgat
    11461 ggtcgtattt tagaagtcat tcgttgcggt tttatcgatg gcaatatttt aattccacaa
    11521 ggtgttatta atgaattaca aattgttgca gattcaaatg acagtgttaa acgtgaaaag
    11581 ggtaaaagag gcttagatat cttaaatgaa ttgtatgatt tagactatcc tacaaaggtt
    11641 atacatccaa ctaaaacaca tagtgatatt gatacaatgc tattaaaact agcaaaacaa
    11701 tatcatgcaa gtattattac aacagatttc aacctaaata aagtatgtca tgtacatggt
    11761 atcaaagcat taaatgttaa tgatttatca gaagcaatca aacctaatgt acatcaaggt
    11821 gatcaacttc atattttact gacaaaaatg ggtaaagagc ctggtcaagc agtaggatat
    11881 ctagatgatg gtacgatggt agttgttgat aatgctaaaa atcttattgg cagtcatgtc
    11941 aatttagaag tagtgagctt attgcaaaca tcttcaggaa gaattgtttt tgctaaaaaa
    12001 atcgaagata cagtatcatt ataaaaactg tagttgacgg aaacaaaata gatattaaaa
    12061 ggtatatgaa atggcgaagt ttgtaatttg aagtataact ataaataaat acaaaacata
    12121 tcagttatca aaaaatacaa atagtgctaa catatcattg aaacaagttc attaagtgtt
    12181 aaattttaga tattaataaa gttattctta tagcaatcaa gacatacaaa attagaatca
    12241 attttaacgt atttgaatag ttaatttgaa gtttaatttt acagtacata atattcattt
    12301 ttaagtcgtt gaaatgcgta tgtagtgcag tcttgattct tagggatagc ttttttaaag
    12361 tgttgaaaaa acaactgctt tctatttaaa atttaagcat attacccagg gaattatgtt
    12421 atgattaaac aatgaaatta ttttaaggtc atgttaaaaa gcattgtttc gttttaacaa
    12481 taagacgagt tacacatttg ttatgacggt cgaagatagc aaatgaacaa tttatatgaa
    12541 ctaacttgaa aatctgatat ttaatttagg agtgaaaaaa atgagcgatc gtataagagt
    12601 aagatatgca ccaagtccaa ctggttatct tcatattggt aatgcaagaa cagcattatt
    12661 caattacttg tatgctaaac attacaacgg agattttgtg attcgaattg aagatactga
    12721 taaaaaacgt aatttagaag atggagaaac atcacaattt gataatctta aatggttagg
    12781 attagattgg gatgagtctg tagataaaga caatggctac ggaccatatc gtcaatctga
    12841 acgtcaacat atctaccaac cattaataga tcagttacta gcagaagata aagcatataa
    12901 atgctatatg acagaagaag aattagaagc tgaacgtgaa gcgcaaatcg ctcgtggtga
    12961 aatgcctcgc tatggtggtc aacatgcgca tttgactgaa gaacaacgtc aacaatttga
    13021 agcagaagga cgccaaccat caattcgttt ccgagtacct caaaatcaaa cgtattcatt
    13081 tgatgatatg gttaaaggga atatttcatt tgattcaaat ggtattggtg actgggttat
    13141 cgttaaaaaa gatggcattc caacgtacaa ttttgcagta gctatagatg atcattacat
    13201 ggaaatttca gatgtcattc gtggtgatga tcatatttca aacacgccta aacaaattat
    13261 gatttatgaa gcatttggct gggagccacc tcgttttggt catatgtcat taattgttaa
    13321 tgaagaacgt aaaaagttaa gtaaacgtga tgggcaaatt ttacaattta ttgagcaata
    13381 tcgtgactta ggttatttac ctgaagcgtt atttaatttt attgcgttat taggttggtc
    13441 tcctgaaggt gaagaagaaa tcttttctaa agaagaattt atcaaaatct ttgatgaaaa
    13501 gcgtttatca aaatcaccag catttttcga taagcaaaaa ttagcatggg ttaataacca
    13561 atatatgaaa caaaaagata ctgaaacagt attccaatta gcattacctc atttaattaa
    13621 agcgaatttg attcctgagg tgccgtcaga agaggattta tcttggggac gcaaattaat
    13681 tgcgctttat caaaaagaaa tgagttatgc cggtgaaatt gtacctttat cagaaatgtt
    13741 ctttaaagaa atgccagctc ttggtgagga agaacaacaa gtgatcaatg gagagcaagt
    13801 accagagtta atgacgcact tattcagtaa attagaagca cttgaaccat ttgaagcggc
    13861 tgaaattaaa aagacaatta aagaagttca aaaagaaaca ggaataaaag gcaagcaatt
    13921 atttatgcct attcgtgttg ctgtaacagg ccaaatgcat ggtcctgaat taccaaatac
    13981 aattgaagta cttggtaaag aaaaagtgct aaaccgttta aaacaatata agtaatgaaa
    14041 tagacaagat caaggtagta tatacttgaa ttttataacg taaccacata tgataaagtt
    14101 gtattaacaa attacaacaa tatgaataga gtactttata taaaacgaag gattagtaat
    14161 taaatttata cgatgcagag agtgtacggt tgctgtgagt acaacgtaga aattaatgaa
    14221 tgcaccttcg taaaatgaat taaatatata atgagagtga tgagcattaa gttgacttag
    14281 tttccttgat aatttggaag cgcccgcaat tttattaatg taattcgcta aattcagagt
    14341 ggaaccgtgc ggaagcgcct ctaacaatac aatttgtatg ttagtggtgc tttttttgat
    14401 atttaatttc gcaggtactt caattatgta tttacgtagt tcaatcattg aggaggaaat
    14461 gatcttgtta aaaagaatga gagacgatat aaaaatggta tttgagcagg atccagcggc
    14521 acgttcaaca ttagaagtca ttacaacgta tgcaggttta catgcagttt ggagtcattt
    14581 gattgcacat aagttataca accaaaaaaa atatgttgca gcacgcgcga tatctcaaat
    14641 ttcaagattt ttcacaggta tagaaatcca tccaggtgct aaaattggaa agcgtctatt
    14701 tatagatcat ggtatgggcg ttgtaatagg agaaacatgt acaattggtg ataatgtgac
    14761 aatctatcaa ggcgtgacac ttggtgggac agggaaagaa agagggaaaa gacacccaga
    14821 tataggagac aatgttttaa tagcagcggg tgcgaaagtt ttaggaaata ttaaaataaa
    14881 ttcaaatgta aatattggtg caaattcagt tgttttacaa tcagttccaa gttattcaac
    14941 ggttgttggt ataccaggac atattgttaa gcaagatggt gttcgagttg gaaaaacatt
    15001 tgatcatcgc catctacctg atccaattta tgaacaaatt aagcatttag aacgacaact
    15061 tgaaaagact aggaatggag agattcaaga tgattacatt atataatacg cttacacgtc
    15121 aaaaagaagt gttcaagcca atagaaccag gaaaggtaaa aatgtatgta tgtggtccta
    15181 cggtatataa ctacattcat attggtaatg caagaccagc aattaattat gacgtagtga
    15241 gacgttactt tgaataccaa ggatataatg tagaatatgt atcaaatttt acagatgtag
    15301 atgataaatt aattaaacgt tctcaagaat taaatcagac tgttcccgaa attgcagaaa
    15361 aatatatcgc tgcttttcat gaagatgttg gtgctttaaa tgttagaaaa gcgacttcaa
    15421 atccaagggt aatggaccat atggatgaca ttattcaatt tattaaagat ttggttgatc
    15481 aaggttatgc atatgaaagt ggtggcgatg tttactttag aacacgtaaa tttgaaggtt
    15541 atggtaaatt aagtcatcaa tccatagatg acttaaaagt gggtgctcgt atagatgcag
    15601 gagagcataa ggaagacgca cttgatttta cattgtggaa aaaagcgaag cctggcgaga
    15661 ttagttggga tagcccattt ggtgaaggga gaccaggatg gcatattgaa tgttctgtaa
    15721 tggcatttca tgagctagga gctacaattg atatacatgc gggtggttca gatttacaat
    15781 ttccacatca tgaaaatgaa atagcacaat cggaagcaca taatcatgcg ccattcgcta
    15841 attattggat gcataatggt tttattaata ttgataatga aaaaatgagt aaatcactag
    15901 gcaactttat tttagttcac gatattatta aagaagttga tccagatgta ttaagattct
    15961 ttatgattag cgtacattat agaagtccaa ttaactataa tctagaattg gttgaatcag
    16021 cacgtagtgg actagagcgt attcgcaata gttatcaatt aattgaagag cgcgcacaaa
    16081 ttgctactaa tattgaaaat caacagacat atattgatca aattgatgcg attttaaatc
    16141 gttttgaaac agttatgaat gatgatttta atacagctaa tgcaattaca gcttggtatg
    16201 atttagcaaa acttgcaaat aaatatgtac tagagaacac aacatcaaca gaagtaattg
    16261 ataaatttaa agcagtttat caaattttca gcgatgtttt aggtgtaccg ttaaaatcta
    16321 aaaaagcaga tgaattatta gatgaagatg ttgaaaaatt aatcgaagag cgtaatgaag
    16381 caaggaaaaa caaagatttt gcacgagcag atgagattcg agacatgctg aaatcacaaa
    16441 acattatatt agaagacaca cctcaagggg ttagatttaa acgtggataa tcaacaagat
    16501 aatcacatta aattattgaa tccattgacc ttagcatata tgggagacgc agtcttagat
    16561 caatatgtac gtacgtatat cgttttaaag cttaaaagta agcctaataa actacatcaa
    16621 atgtctaaaa aatatgtatc tgccaaaagt caggcgcaaa cgttagaata tttaatggag
    16681 caagaatggt ttacagacga agaaatggat attttgaaac gagggcgtaa cgcgaaaagt
    16741 catactaaag ctaaaaatac tgatgttcaa acatatcgta aaagttcagc gatagaagca
    16801 gtgataggtt ttctttattt agaaaaaaga gaagaacgat tagaggcatt attaaatgaa
    16861 ataataacaa tagtaaacga aaggtagtga caatgtggaa gatacggtta ttgttggtag
    16921 gcatgctgtt agagaagcga ttattactgg gcatccgata aataagatat tgattcaaga
    16981 aggaattaaa aagcaacaaa ttaatgaaat tttaaaaaat gcaaaagatc aaaaaatcat
    17041 tgttcaaact gtaccaaaat ctaaattaga ttttttagca aatgcaccac atcagggggt
    17101 tgcagcgctt attgcaccat atgaatatgc tgacttcgat caatttttaa aacagcaaaa
    17161 agaaaaagaa ggtttatcga cagtgcttat attagacggc ttagaagacc cgcataactt
    17221 gggatcaatt ttaagaacag cagatgcaac gggagttgat ggtgttatta ttcctaaacg
    17281 tcgttcagtt acactaacgc aaacagttgc aaaagcctca acaggtgcaa ttgaacatgt
    17341 accagttatt cgagtgacaa atttagctaa aactatcgat gaactaaaag ataatggttt
    17401 ttgggtagct ggcactgaag ctaataatgc aacagattat agaaatcttg aagcggacat
    17461 gtcattggct attgtaattg gcagcgaagg acagggtatg agtcgtctag taagtgataa
    17521 atgcgatttt tatattaaga ttccaatggt tggccatgta aacagtttga atgcttcggt
    17581 tgcagcaagt ttaatgatgt acgaagtatt tcgaaaaaga catgatgttg gagaaatata
    17641 atgaaagaac gttacttaat cattgatgga tacaatatga taggacaatc accaacgcta
    17701 agcgccattg caaaagagaa tttagaagaa gctagaatgc aattaataga tacaattgca
    17761 aattataatg cagttatttc agatgaaatt atttgtgttt tcgatgctta tgaccaatcg
    17821 ggtgttgaaa gagaatacat gtatcatggc gttaaaacaa tttttaccaa ggaaaaagaa
    17881 acagctgata gtttcataga acgttatgtc tatgaacttt atgacaagca cactaagcat
    17941 attacagttg taacaagtga tatgagtgag caacatgcta tctttggatc aggtgcatat
    18001 agaatatcat ctcgcgaaat gtggagagat ttaaaagaaa atgaaattga tgtgagtaaa
    18061 tcattagatg atataagtga aaacaagcca agaactcgaa ttccgttatc ttctgaaatc
    18121 cttgcagaat ttgaaaaaat acgaagagga catcataaga aatgacattt ccatcatctt
    18181 gaaattttga atgtaacaat attaaactaa ccttaaattt tagatagaag gggttagttt
    18241 aatacttgaa atatgatttg acaactcaag acagtacaat caaacgtaac aatgcaataa
    18301 atgataaaga cttcgaaaag ttagtaatgg atctacaacc attaattata cggcgcatca
    18361 aaacatttgg atttaatcat tatgatttag aagatttata tcaagaaata cttatacgga
    18421 tgtataggtc ggtccaaaca tttgatttta gtgcagagca gcctttcaca aattatgttc
    18481 aatgtttaat tacgtctgta aagtatgatt atttgagaaa atatttagct acaaataaaa
    18541 gaatggataa tttgattaat gaatatagag ttacgtatcc atgtgcaata aagcgttatg
    18601 atgttgaaaa caattatttg aataaattag caattaaaga gttgattcgt cagtttaagt
    18661 atttgagtgc atttgaaaaa gatgtcatgt atttaatgtg tgaacaatat aagccgagag
    18721 aaattgctca actgatgcat gtaaaagaga aagtgattta taatgccata caacgatgta
    18781 aaaataaaat aaaacgttat ttcaaaatga tttgaaaagc gtcttaggac gtgaattgaa
    18841 ttagaacgtg ttacttcctg atggtttgac atttgttata aattttatgt atagtatact
    18901 ggtattataa tgaataaagg tgaattattg tgaggaaaat acctttaaat tgtgaagctt
    18961 gtggcaatag aaattataat gttcctaagc aagaaggcac ggcaacaaga ttaaccttaa
    19021 agaaatattg tccaaaatgt aacgcgcaca caattcataa agaatcgaaa taaatacatt
    19081 cgaaataata ctttgataat atgttcaaag gatttggagg ttgagcagat ggctaaaaaa
    19141 gaaagtttct ttaaaggcgt taagtctgaa atggaaaaaa caagttggcc gacgaaagaa
    19201 gagctattta aatatactgt aattgtagtt tctactgtta tattcttctt agtctttttc
    19261 tatgccttag atttaggaat tacagcattg aaaaatttat tatttggtta gaggagtgaa
    19321 gacatgtctg aagaagttgg cgcaaagcgt tggtatgcag tgcatacata ttctggatat
    19381 gaaaataaag ttaaaaagaa tttagaaaaa agagtagaat ctatgaatat gactgaacaa
    19441 atctttagag tagtcatacc ggaagaagaa gaaactcaag taaaagatgg caaagctaaa
    19501 acgactgtta aaaaaacatt ccctggatat gttttagtgg aattaatcat gacagatgaa
    19561 tcatggtatg tggtaagaaa tacaccaggc gttactggtt ttgtaggttc tgcaggtgca
    19621 gggtctaagc caaatccatt gttaccagaa gaagttcgct tcatcttaaa acaaatgggt
    19681 cttaaagaaa agactatcga tgttgaactc gaagttggcg agcaagttcg tattaaatca
    19741 ggtccatttg cgaatcaagt tggtgaagtt caagaaattg aaacagataa gtttaaacta
    19801 acagtattag tagatatgtt tggccgagaa acaccagtag aagttgaatt cgatcaaata
    19861 gaaaagcttt aattaacaat taaagttatt aaactaacca aaagataaaa aagagtattg
    19921 attttttaat tagaaaagtg ttaaaattat gtggtcgcgc ttttagagcg cccatttcgt
    19981 cacgaaatgt taagagtggg agggcaaaac tgagccctgt gaccacatca cgatatcaag
    20041 gaggtgcaca tcgtggctaa aaaagtagat aaagttgtta aattacaaat tcctgcaggt
    20101 aaagcgaatc cagcaccacc agttggtcca gcattaggtc aagcaggtgt gaacatcatg
    20161 ggattctgta aagagttcaa tgcacgtact caagatcaag caggtttaat tattccggta
    20221 gaaatcagtg tttatgaaga tcgttcattt acatttatta caaaaactcc accggctcca
    20281 gtattactta aaaaagcagc tggtattgaa aaaggttcag gtgaaccaaa caaaactaaa
    20341 gttgctacag taactaaaga tcaagtacgc gaaattgcta acagcaaaat gcaagactta
    20401 aacgctgctg acgaagaagc agctatgcgt attatcgaag gtactgcacg tagtatgggt
    20461 atcgttgtag aataatttta cgaatattaa atttgattac atgatttaaa cgatgaagca
    20521 gataacagag ataataatga tgaattataa atataatctg aatgactaga ttaatgattg
    20581 atttattcat atgattaatt cttctgttgt ctgctcttaa cttgcatcta gcaagtaatg
    20641 tgggaggaaa ttccgctaaa accactaaag gaggaactat aaatggctaa aaaaggtaaa
    20701 aagtatcaag aagcagctag taaagttgac cgtactcagc actacagtgt tgaagaagca
    20761 attaaattag ctaaagaaac aagcattgct aactttgacg cttctgttga agttgcattc
    20821 cgtttaggaa ttgatacacg taaaaatgac caacaaatcc gtggtgcagt tgtattacca
    20881 aacggaactg gtaaatcaca aagtgtatta gtattcgcta aaggtgacaa aattgctgaa
    20941 gctgaagcag caggtgctga ctatgtaggt gaagcagaat acgttcaaaa aatccaacaa
    21001 ggttggttcg acttcgatgt agtagttgct acaccagaca tgatgggtga agttggtaaa
    21061 ttaggtcgtg tattaggacc aaaaggttta atgccaaacc ctaaaactgg aactgtaaca
    21121 atggatgtta aaaaagctgt tgaagaaatc aaagctggta aagtagaata ccgtgctgaa
    21181 aaagctggta tcgtacatgc atcaattggt aaagtttcat ttactgatga acaattaatt
    21241 gaaaacttca atactttaca agatgtatta gctaaagcta aaccatcatc tgctaaaggt
    21301 acatacttca aatctgttgc tgtaactaca acaatgggtc ctggagttaa aattgatact
    21361 gcaagtttat aataataaat gatataaaca attacaggct gaaagaaata tctttcagtc
    21421 tgtaaaaata tattgacaat aagtaatttc caagttatat tacttattgt gattatttta
    21481 cctaagacag taggagttat ttataactta aaatttatcc tgccgaggct aaaattgact
    21541 tgaacgtgat gatctatgat ctttcaagca ctttttgccg tgggtagaaa gtgctttttt
    21601 tattaatttt aaaaaaagca ccaaaaattt aaatggaggt gtctgaatgt ctgctatcat
    21661 tgaagctaaa aaacaactag ttgatgaaat tgctgaggta ctatcaaatt cagtttcaac
    21721 agtaatcgtt gactaccgtg gattaacagt agctgaagtt actgacttac gttcacaatt
    21781 acgtgaagct ggtgttgagt ataaagtata caaaaacact atggtacgtc gtgcagctga
    21841 aaaagctggt atcgaaggct tagatgaatt cttaacaggt cctactgcta ttgcaacttc
    21901 aagtgaagat gctgtagctg cagcgaaagt aatttctgga tttgctaaag atcatgaagc
    21961 attagaaatt aaatcaggcg ttatggaagg caatgttatt acagcagaag aagttaaaac
    22021 tgttggttca ttaccttcac acgatggtct tgtatctatg cttttatcag tattacaagc
    22081 tcctgtacgc aacttcgctt atgcggttaa agctattgga gaacaaaaag aagaaaacgc
    22141 tgaataattt ttagcgtaaa aaaattaaaa ataatggagg aattataaaa tggctaatca
    22201 tgaacaaatc attgaaacga ttaaagaaat gtcagtatta gaattaaacg acttagtaaa
    22261 agcaattgaa gaagaatttg gtgtaactgc agctgctcca gtagcagtag caggtgcagc
    22321 tggtggcgct gacgctgcag cagaaaaaac tgaatttgac gttgagttaa cttcagctgg
    22381 ttcatctaaa atcaaagttg ttaaagctgt taaagaagca actggtttag gattaaaaga
    22441 tgctaaagaa ttagtagacg gagctcctaa agtaatcaaa gaagctttac ctaaagaaga
    22501 agctgaaaaa cttaaagaac aattagaaga agttggagct actgtagaat taaaataatt
    22561 caagtatctt aaacttaata atcaaagttt tatagcaagt attgctataa tataatgatt
    22621 ctttgagaag ttaaaacccc gttattttga taacggggtt ttattcattt aaagactaag
    22681 tgaaatgtta taattataat gacgagttac aaagtgaaga tgaggtggaa taatgagtca
    22741 ttattacgat gaagatccaa gtgtaattag caatgaacaa cgtattcaat atcaattaaa
    22801 ccatcataaa attaatttaa taactgataa cggagtgttt tcgaaagata aagtagatta
    22861 tggttcagat gttcttgttc aaactttttt aaaagcgcat ccacctggtc caagtaagcg
    22921 aattgccgat gttggttgtg gttacggacc aattggtttg atgattgcta aagtatcacc
    22981 acatcattca attacaatgc tagatgttaa tcacagagcg ctagccttag ttgaaaaaaa
    23041 caaaaaatta aatggtattg ataatgtgat cgtaaaggaa agtgatgctt tgtctgctgt
    23101 ggaagacaaa agttttgatt ttattttaac caatccacca ataagagcag ggaaagaaac
    23161 cgtgcatcgt atattcgagc aagcattaca tagattagac tcgaacggtg aactatttgt
    23221 tgtaattcag aagaagcaag gtatgccatc tgcaaagaaa agaatgaatg aactttttgg
    23281 aaatgtagaa gtggtaaata aagataaagg atattacatt ctgagaagta taaaagcttg
    23341 aaatgaaatg gatattctgt tatagttatt taatgtaaaa atttatgttc aataagtgtg
    23401 tacttttacg ttaaatagat aagttaatta agaataaata tagaatcgaa aatggtgtca
    23461 tcattagtgt tgccgttttc tttttgtctt tttattaata tgcttatggt atttagctaa
    23521 aagcggatca cataattttt gaggggtgaa tctgtttggc aggtcaagtt gtccaatatg
    23581 gaagacatcg taaacgtaga aactacgcga gaatttcaga agtattagaa ttaccaaact
    23641 taatagaaat tcaaactaaa tcttacgagt ggttcctaag agaaggttta atcgaaatgt
    23701 ttagagacat ttctccaatt gaagatttta ctggtaattt gtcattagag tttgtggatt
    23761 accgtttagg agaaccaaaa tatgatttag aagaatctaa aaaccgtgac gctacttatg
    23821 ctgcacctct tcgtgtaaaa gtgcgtctaa tcattaaaga aacaggagaa gttaaagaac
    23881 aagaagtctt tatgggtgat ttcccattaa tgactgatac aggtacgttc gttatcaatg
    23941 gtgcagaacg tgtaatcgta tctcaattag ttcgttcacc atccgtttat ttcaatgaaa
    24001 aaatcgacaa aaatggtcgt gaaaactatg atgcaacaat tattccaaac cgaggtgcat
    24061 ggttagaata tgaaacagat gctaaagatg ttgtatacgt gcgtattgat agaacacgta
    24121 aactaccatt aacagtattg ttacgtgcat taggtttctc aagtgaccaa gaaattgttg
    24181 accttttagg tgacaatgaa tatttacgta atactttaga gaaagacggc actgaaaaca
    24241 ctgaacaagc gttattagaa atctatgaac gtttacgtcc aggtgaacca ccaactgttg
    24301 aaaatgctaa aagtctattg tattcacgtt tctttgatcc aaaacgctat gacttagcaa
    24361 gcgtgggtcg ttataaaaca aacaaaaaat tacatttaaa acatcgttta ttcaatcaaa
    24421 aattagctga gccaattgtg aatactgaaa ctggtgaaat tgtagttgaa gaaggtacag
    24481 tgcttgatcg tcgtaaaatc gacgaaatca tggatgtact tgaatcaaac gcaaacagcg
    24541 aagtgtttga attgcatggt agcgttatag acgagccagt agaaattcaa tcaattaaag
    24601 tatatgttcc taacgatgat gaaggtcgta cgacaactgt aattggtaat gctttccctg
    24661 actcagaagt taaatgtatt acaccggcag atatcatcgc ttcaatgagt tacttcttta
    24721 acttattaag tggtattgga tatacagatg atattgacca tttaggtaac cgtcgtttac
    24781 gttctgtagg tgaattacta caaaaccaat tccgtatcgg tttatcaaga atggaaagag
    24841 ttgtacgtga aagaatgtca attcaagata ctgagtctat cacacctcaa caattaatta
    24901 atattcgacc tgttattgca tctattaaag aattctttgg tagctctcaa ttatcacaat
    24961 tcatggacca agcaaatcca ttagctgagt taacgcataa acgtcgtcta tcagcattag
    25021 gacctggtgg tttaacacgt gaacgtgctc aaatggaagt gcgtgacgtt cactactctc
    25081 actatggccg tatgtgtcca attgaaacgc ctgagggacc aaacattgga ttgattaact
    25141 cattatcaag ttatgcacgt gtaaatgaat tcggctttat tgaaacacca tatcgtaaag
    25201 ttgatttaga tacacatgct atcactgatc aaattgacta tttaacagct gacgaagaag
    25261 atagctatgt tgtagcacaa gcaaactcta aattagatga aaatggtcgt ttcatggatg
    25321 atgaagttgt atgtcgtttc cgtggtaaca atacagttat ggctaaagaa aaaatggatt
    25381 atatggatgt atcgccgaag caagttgttt cagcagcgac agcatgtatt ccattcttag
    25441 aaaatgatga ctcaaaccgt gcattgatgg gtgcgaacat gcaacgtcaa gcagtgcctt
    25501 tgatgaatcc agaagcacca tttgttggta caggtatgga acacgttgca gcacgtgatt
    25561 ctggtgcagc tattacagct aagcacagag gtcgtgttga acatgttgaa tctaatgaaa
    25621 ttcttgtacg tcgtctagtt gaagagaacg gcgttgagca tgaaggtgaa ttagatcgct
    25681 atccattagc taaatttaaa cgttcaaact caggtacatg ttacaaccaa cgtccaatcg
    25741 ttgcagttgg agatgttgtt gagtttaacg agattttagc agatggacca tctatggaat
    25801 taggagaaat ggcattaggt agaaacgtag tagttggttt catgacttgg gacggttaca
    25861 actatgagga tgccgttatc atgagtgaaa gacttgtgaa agatgacgtg tatacttcta
    25921 ttcatattga agagtatgaa tcagaagcac gtgatactaa gttaggacct gaagaaatca
    25981 caagagatat tcctaatgtt tctgaaagtg cacttaagaa cttagacgat cgtggtatcg
    26041 tttatattgg tgcagaagta aaagatggag atattttagt tggtaaagta acgcctaaag
    26101 gtgtaactga gttaactgcc gaagaaagat tgttacatgc aatctttggt gaaaaagcac
    26161 gtgaagttag agatacttca ttacgtgtac ctcacggcgc tggcggtatc gttcttgatg
    26221 taaaagtatt caatcgtgaa gaaggcgacg acacattatc acctggtgta aaccaattag
    26281 tacgtgtata tatcgttcaa aaacgtaaaa ttcatgttgg tgataagatg tgtggtcgac
    26341 atggtaacaa aggtgtcatt tctaagattg ttcctgaaga agatatgcct tacttaccag
    26401 atggacgtcc gattgatatc atgttaaatc ctcttggtgt accatctcgt atgaacatcg
    26461 gacaagtatt agagctacac ttaggtatgg ctgctaaaaa tcttggtatt cacgttgcat
    26521 caccagtatt tgacggtgca aacgatgacg atgtatggtc aacaattgaa gaagctggta
    26581 tggctcgtga tggtaaaact gtactttatg atggacgtac aggtgaacca ttcgataacc
    26641 gtatttcagt aggtgtaatg tacatgttga aacttgcgca catggttgat gataaattac
    26701 atgcgcgttc aacaggacca tattcacttg ttacacaaca accacttggc ggtaaagcgc
    26761 aattcggtgg acaacgtttc ggtgagatgg aggtatgggc acttgaagca tatggtgctg
    26821 catacacatt acaagaaatc ttaacttaca aatccgatga tacagtagga cgtgtgaaaa
    26881 catacgaggc tattgttaaa ggtgaaaaca tctctagacc aagtgttcca gaatcattcc
    26941 gagtattgat gaaagaatta caaagtttag gtttagatgt aaaagttatg gatgagcaag
    27001 ataatgaaat cgaaatgaca gacgttgatg acgatgatgt tgtagaacgc aaagtagatt
    27061 tacaacaaaa tgatgctcct gaaacacaaa aagaagttac tgattaatac gcaatttaca
    27121 aaacaggcaa aaagatacta agctgaattt tattgatgat tcggtttagt actttaagcc
    27181 attttaaata aatgcaaatc aatcaaatag cacagctaat ctaaattgaa ggaggtaggc
    27241 tccttgattg atgtaaataa tttccattat atgaaaatag gattggcttc acctgaaaaa
    27301 atccgttctt ggtcttttgg tgaagttaaa aaacctgaaa caatcaacta ccgtacatta
    27361 aaacctgaaa aagatggtct attctgtgaa agaattttcg gacctacaaa agactgggaa
    27421 tgtagttgtg gtaaatacaa acgtgttcgc tacaaaggca tggtctgtga cagatgtgga
    27481 gttgaagtaa ctaaatctaa agtacgtcgt gaaagaatgg gtcacattga acttgctgct
    27541 ccagtttctc acatttggta tttcaaaggt ataccaagtc gtatgggatt attacttgac
    27601 atgtcaccaa gagcattaga agaagttatt tactttgctt cttatgttgt tgtagatcca
    27661 ggtccaactg gtttagaaaa gaaaacttta ttatctgaag ctgaattcag agattattat
    27721 gataaatacc caggtcaatt cgttgcaaaa atgggtgcag aaggtattaa agatttactt
    27781 gaagagattg atcttgacga agaacttaaa ttgttacgcg atgagttgga atcagctact
    27841 ggtcaaagac ttactcgtgc aattaaacgt ttagaagttg ttgaatcatt ccgtaattca
    27901 ggtaacaaac cttcatggat gattttagat gtacttccaa tcatcccacc agaaattcgt
    27961 ccaatggttc aattagatgg tggacgattt gcaacaagtg acttaaacga cttataccgt
    28021 cgtgtaatta atcgaaataa tcgtttgaaa cgtttattag atttaggtgc acctggtatc
    28081 atcgttcaaa acgaaaaacg tatgttacaa gaagccgttg acgctttaat tgataatggt
    28141 cgtcgtggtc gtccagttac tggcccaggt aaccgtccat taaaatcttt atctcatatg
    28201 ttaaaaggta aacaaggtcg tttccgtcaa aacctacttg gtaaacgtgt tgactattca
    28261 ggacgttcag ttatcgcggt aggtccaagc ttgaaaatgt accaatgtgg tttaccgaaa
    28321 gaaatggcac ttgaactatt taaaccattt gtaatgaaag aattagttca acgtgaaatt
    28381 gcaactaaca ttaaaaatgc gaagagtaaa atcgaacgca tggatgatga agtttgggac
    28441 gtattggaag aagtaattag agaacatcct gtattactta accgtgcacc aacacttcat
    28501 agacttggta ttcaagcatt tgaaccaact ttagttgaag gtcgtgcgat tcgtctacat
    28561 ccacttgtaa caacagctta taacgctgac tttgatggtg accaaatggc ggttcacgtt
    28621 cctttatcaa aagaggcaca agctgaagca agaatgttga tgttagcagc acaaaacatc
    28681 ttgaacccta aagatggtaa accagtagtt acaccatcac aagatatggt acttggtaac
    28741 tattacctta ctttagaaag aaaagatgca gtaaatacag gcgcaatctt taataataca
    28801 aatgaagtgt taaaagcata tgcaaatggc tttgtacatt tacacacaag aattggtgta
    28861 catgcaagtt cattcaacaa cccaacattt actgaagaac aaaacaaaaa gattcttgct
    28921 acgtcagtag gtaaaattat attcaatgaa atcattccgg attcatttgc ttatattaat
    28981 gaacctacgc aagaaaactt agaaagaaag acaccaaaca gatacttcat cgatcctaca
    29041 actttaggtg aaggtggatt aaaagaatac tttgaaaatg aagaattaat tgaacctttc
    29101 aacaaaaaat tcttaggtaa tattattgca gaagtattca acagatttaa catcactgat
    29161 acatcaatga tgttagaccg tatgaaagac ttaggattca aattctcatc taaagctggt
    29221 attacagtag gtgttgctga tatcgtagta ttacctgata agcaacaaat acttgatgag
    29281 catgaaaaat tagtcgacag aattacaaaa caattcaatc gtggtttaat cactgaagaa
    29341 gaaagatata atgcagttgt tgaaatttgg acagatgcaa aagatcaaat tcaaggtgaa
    29401 ttgatgcaat cacttgataa aactaaccca atcttcatga tgagtgattc aggtgcccgt
    29461 ggtaacgcat ctaactttac acagttagca ggtatgcgtg gattgatggc cgcaccatct
    29521 ggtaagatta tcgaattacc aatcacatct tcattccgtg aaggtttaac agtacttgaa
    29581 tacttcatct caactcacgg tgcgcgtaaa ggtcttgccg atacagcact taagacagct
    29641 gactcaggat atcttactcg tcgtcttgtt gacgtggcac aagatgttat tgttcgtgaa
    29701 gaagactgtg gtacagatag aggtttatta gtttctgata ttaaagaagg tacagaaatg
    29761 attgaaccat ttatcgaacg tattgaaggt cgttattcta aagaaacaat tcgtcatcct
    29821 gaaactgatg aagttatcat tcgtcctgat gaattaatta cacctgaaat tgctaagaaa
    29881 attacagatg ctggtattga acaaatgtat attcgctcag catttacttg taacgcaaga
    29941 cacggtgttt gtgaaaaatg ttacggtaaa aaccttgcta ctggtgaaaa agttgaagtt
    30001 ggtgaagcag ttggtacaat tgcagcccaa tctatcggtg aaccaggtac acagcttaca
    30061 atgcgtacat tccatacagg tggggtagca ggtagcgata tcacacaagg tcttcctcgt
    30121 attcaagaga ttttcgaagc acgtaaccct aaaggtcaag cggtaattac ggaaatcgaa
    30181 ggtgtcgtag aagatattaa attagcaaaa gatagacaac aagaaattgt tgttaaaggt
    30241 gctaatgaaa caagatcata tcttgcttca ggtacttcaa gaattattgt agaaatcggt
    30301 caaccagtac aacgtggtga agtattaact gaaggttcta ttgaacctaa gaattactta
    30361 tctgttgctg gattaaacgc gactgaaagc tacttattaa aagaagtaca aaaagtttac
    30421 cgtatgcaag gggtagaaat cgacgataaa cacgttgagg ttatggttcg acaaatgtta
    30481 cgtaaagtta gaattatcga agcaggtgat acgaagttat taccaggttc attagttgat
    30541 attcacaact ttacagatgc aaatagagaa gcatttaaac accgcaagcg ccctgcaaca
    30601 gctaaaccag tattacttgg tattactaaa gcatcacttg aaacagaaag tttcttatct
    30661 gcagcatcat tccaagaaac aacaagagtt ctaacagatg cagcaattaa aggtaagcgt
    30721 gatgacttat taggtcttaa agaaaacgta attatcggta agctaattcc agctggtact
    30781 ggtatgagac gttatagcga cgtaaaatac gaaaaaacag ctaaaccagt tgcagaagtt
    30841 gaatctcaaa ctgaagtaac ggaataacaa gtatataaca gaggctaagg ctttagcctc
    30901 ttgttatttt tatgtaaatt atttgattta atgttgacga attctcttgt tcaatgttaa
    30961 tatattaaag gttgatgcaa gcagaacttt ggaggataaa ttattgtcta aggaaaaagt
    31021 tgcacgcttt aacaaacaac attttgtagt tggtcttaaa gaaacgctta aagcgttaaa
    31081 gaaagatcaa gttacatctt tgattattgc tgaagacgtt gaagtatatt taatgactcg
    31141 cgtgttaagc caaatcaatc agaaaaatat acctgtatct tttttcaaaa gcaaacatgc
    31201 tttgggtaaa catgtaggta ttaacgtcaa tgcgacaata gtagcattga ttaaatgaga
    31261 attagtaagt gtttaactta ctaaatttta tttaacctaa aaatgaacca cctggatgtg
    31321 tgggattaaa aagtgaagag aggaggacat atcacatgcc aactattaac caattagtac
    31381 gtaaaccaag acaaagcaaa atcaaaaaat cagattctcc agctttaaat aaaggtttca
    31441 acagtaaaaa gaaaaaattt actgacttaa actcaccaca aaaacgtggt gtatgtactc
    31501 gtgtaggtac aatgacacct aaaaaaccta actcagcgtt acgtaaatat gcacgtgtgc
    31561 gtttatcaaa caacatcgaa attaacgcat acatccctgg tatcggacat aacttacaag
    31621 aacacagtgt tgtacttgta cgtggtggac gtgtaaaaga cttaccaggt gtgcgttacc
    31681 atattgtacg tggagcactt gatacttcag gtgttgacgg acgtagacaa ggtcgttcat
    31741 tatacggaac taagaaacct aaaaactaag aatttagttt ttaattaaat cttaaactta
    31801 aaatatttaa tataaggaag ggaggattta cattatgcct cgtaaaggat cagtacctaa
    31861 aagagacgta ttaccagatc caattcataa ctctaagtta gtaactaaat taattaacaa
    31921 aattatgtta gatggtaaac gtggaacagc acaaagaatt ctttattcag cattcgacct
    31981 agttgaacaa cgcagtggtc gtgatgcatt agaagtattc gaagaagcaa tcaacaacat
    32041 tatgccagta ttagaagtta aagctcgtcg tgtaggtggt tctaactatc aagtaccagt
    32101 agaagttcgt ccagagcgtc gtactacttt aggtttacgt tggttagtta actatgcacg
    32161 tcttcgtggt gaaaaaacga tggaagatcg tttagctaac gaaattttag atgcagcaaa
    32221 taatacaggt ggtgccgtta agaaacgtga ggacactcac aaaatggctg aagcaaacaa
    32281 agcatttgct cactaccgtt ggtaagataa aagcttttac cctgagtgtg ttctatatta
    32341 atgaattttc attaagcgtt catgcttagg gcatcgccat atctatcgta tttattcagt
    32401 aatataaact ggaaggagaa aaaatacatg gctagagaat tttcattaga aaaaactcgt
    32461 aatatcggta tcatggctca cattgatgct ggtaaaacga ctacgactga acgtattctt
    32521 tattacactg gccgtatcca caaaattggt gaaacacatg aaggtgcttc acaaatggac
    32581 tggatggagc aagaacaaga ccgtggtatt actatcacat ctgctgcaac aacagcagct
    32641 tgggaaggtc accgtgtaaa cattatcgat acacctggac acgtagactt cactgtagaa
    32701 gttgaacgtt cattacgtgt acttgacgga gcagttacag tacttgatgc acaatcaggt
    32761 gttgaacctc aaactgaaac agtttggcgt caggctacaa cttatggtgt tccacgtatc
    32821 gtatttgtaa acaaaatgga caaattaggt gctaacttcg aatactctgt aagtacatta
    32881 catgatcgtt tacaagctaa cgctgctcca atccaattac caattggtgc ggaagacgaa
    32941 ttcgaagcaa tcattgactt agttgaaatg aaatgtttca aatatacaaa tgatttaggt
    33001 actgaaattg aagaaattga aattcctgaa gaccacttag atagagctga agaagctcgt
    33061 gctagcttaa tcgaagcagt tgcagaaact agcgacgaat taatggaaaa atatcttggt
    33121 gacgaagaaa tttcagtttc tgaattaaaa gaagctatcc gccaagctac tactaacgta
    33181 gaattctacc cagtactttg tggtacagct ttcaaaaaca aaggtgttca attaatgctt
    33241 gacgctgtaa ttgattactt accttcacca ctagacgtta aaccaattat tggtcaccgt
    33301 gctagcaacc ctgaagaaga agtaatcgct aatgcagacg attcagctga attcgctgca
    33361 ttagcgttca aagttatgac tgacccttat gttggtaaat taacattctt ccgtgtgtac
    33421 tcaggtacaa tgacatctgg ttcatacgtt aagaactcta ctaaaggtaa acgtgaacgt
    33481 gtaggtcgtt tattacaaat gcacgctaac tcacgtcaag aaatcgatac tgtatactct
    33541 ggagatatcg ctgctgcggt aggtcttaaa gatacaggta ctggtgatac tttatgtggt
    33601 gagaaaaatg acattatctt ggaatcaatg gaattcccag agccagttat tcacttatca
    33661 gtagagccaa aatctaaagc tgaccaagat aaaatgactc aagctttagt taaattacaa
    33721 gaagaagacc caacattcca tgcacacact gacgaagaaa ctggacaagt tatcatcggt
    33781 ggtatgggtg agcttcactt agacatctta gtagaccgta tgaagaaaga attcaacgtt
    33841 gaatgtaacg taggtgctcc aatggtttca tatcgtgaaa cattcaaatc atctgcacaa
    33901 gttcaaggta aattctctcg tcaatctggt ggtcgtggtc aatacggtga tgttcacatt
    33961 gaattcacac caaacgaaac aggcgcaggt ttcgaattcg aaaacgctat cgttggtggt
    34021 gtagttcctc gtgaatacat tccatcagta gaagctggtc ttaaagatgc tatggaaaat
    34081 ggtgtcttag caggttatcc attaattgat gttaaagcta aattatatga tggttcatac
    34141 catgatgtcg attcatctga aatggccttc aaaattgctg catcattagc acttaaagaa
    34201 gctgctaaaa aatgtgatcc tgtaatctta gaaccaatga tgaaagtaac tattgaaatg
    34261 cctgaagagt acatgggtga tatcatgggt gacgtaacat ctcgtcgtgg acgtgttgat
    34321 ggtatggaac ctcgtggtaa tgcacaagtt gttaatgctt atgtaccact ttcagaaatg
    34381 ttcggttatg caacatcatt acgttcaaac actcaaggtc gcggtactta cactatgtac
    34441 ttcgatcact atgctgaagt tccaaaatca atcgctgaag atattatcaa gaaaaataaa
    34501 ggtgaataat ataacttgtt ttgactagct agcctaggtt aaaatacaag gtgagcttaa
    34561 atgtaagcta tcatctttat agtttgattt tttggggtga atgcattata aaagaattgt
    34621 aaaattcttt ttgcatcgct ataaataatt tctcatgatg gtgagaaact atcatgagag
    34681 ataaatttaa atattatttt taattagaat aggagagatt ttataatggc aaaagaaaaa
    34741 tttgatcgtt ctaaagaaca tgccaatatc ggtactatcg gtcacgttga ccatggtaaa
    34801 acaacattaa cagcagcaat cgctactgta ttagcaaaaa atggtgactc agttgcacaa
    34861 tcatacgaca tgattgacaa cgctccagaa gaaaaagaac gtggtatcac aatcaatact
    34921 tcacacattg agtaccaaac tgacaaacgt cactacgctc acgttgactg cccaggacac
    34981 gctgactatg ttaaaaacat gatcactggt gctgctcaaa tggacggtgg tatcttagta
    35041 gtatctgctg ctgacggtcc aatgccacaa actcgtgaac acattctttt atcacgtaac
    35101 gttggtgtac cagcattagt agtattctta aacaaagttg acatggttga cgatgaagaa
    35161 ttattagaat tagtagaaat ggaagttcgt gacttattaa gcgaatatga cttcccaggt
    35221 gacgatgtac ctgtaatcgc tggttcagca ttaaaagctt tagaaggcga tgctcaatac
    35281 gaagaaaaaa tcttagaatt aatggaagct gtagatactt acattccaac tccagaacgt
    35341 gattctgaca aaccattcat gatgccagtt gaggacgtat tctcaatcac tggtcgtggt
    35401 actgttgcta caggccgtgt tgaacgtggt caaatcaaag ttggtgaaga agttgaaatc
    35461 atcggtttac atgacacatc taaaacaact gttacaggtg ttgaaatgtt ccgtaaatta
    35521 ttagactacg ctgaagctgg tgacaacatt ggtgcattat tacgtggtgt tgctcgtgaa
    35581 gacgtacaac gtggtcaagt attagctgct cctggttcaa ttacaccaca tactgaattc
    35641 aaagcagaag tatacgtatt atcaaaagac gaaggtggac gtcacactcc attcttctca
    35701 aactatcgtc cacaattcta tttccgtact actgacgtaa ctggtgttgt tcacttacca
    35761 gaaggtactg aaatggtaat gcctggtgat aacgttgaaa tgacagtaga attaatcgct
    35821 ccaatcgcga ttgaagacgg tactcgtttc tcaatccgtg aaggtggacg tactgtagga
    35881 tcaggcgttg ttactgaaat cattaaataa tttctaattt cttagatttt atataaaaag
    35941 aagatccctc aatcgagggg tctttttttt aatgtgtaaa ttttgtaatg gctgttcaat
    36001 ttagaagaac aataattgat gagaggttga ctaataaaac ttataactga taatacagtt
    36061 taaataaaat tgttgagcct tggacattgt aaaatgctcc tctcaaagtt ttcatttttc
    36121 aatgtctact ttgaaggagc attacattag tttatgtctc agattcatat ctttcaatta
    36181 atttaaatgc ttaatttgtt ttaaatactt gctctaattc aatgattttc aaaaatacag
    36241 ctacagcgta ttttaatgat ttttcatcaa tatcaaattt gggattatgg tgtggcgctg
    36301 taataccttt actttcatta ccgcaaccag tcagaaagaa tgcacctggt cgtactttca
    36361 aataatgtga aaaatcttct ccaatcatca ttaaatctga ttcattaaag cgtacatgta
    36421 agtcatttgt tgcttcttta ataacttgat atgctttctc gttgttatgg acaggcaagt
    36481 atcctttaat ataattcaag tcatattcaa tgtcatttgc aattgccaaa ccttgtagta
    36541 gcttatccat tttatgcatg acatgttctt gtattttcga gtcaaatgta cgaactgtac
    36601 ctttacaaaa tgcttgatca ggaataacgc tatctgtggt gcctgcttga atcattccaa
    36661 atgaaagtac agcttgttta actggatcaa tcgtacgtga aattattttt tgtgcactta
    36721 aaatgaactc tgccatgatt actattgggt caatggtttc atgaggtttg gcaccatgac
    36781 cgccacgacc tttaattgtg acactaaatt catctggaga ggccatgatt gcccccgcac
    36841 gcgaatgaat agttccagta ggataaccac tccataaatg tgtaccgtaa attctatcta
    36901 cattttccag acatccagca tcaatcattt cttgagaacc acctggcatg atttcttcac
    36961 cgtactggaa tattaataca acattacctt ctaataaatg tttatgttca tctaaaattt
    37021 ctgctacggt aagtaaaatt gctgtatgac catcatgccc acacgcatgc atacatcctg
    37081 gatttttaga cttataaggc acatcgttta attcctcgac aggtaacgca tcaaagtcag
    37141 ctcttaatgc aatggtaggt cctgtgccca aacctttaaa tgtggctttg ataccattgc
    37201 ggccgatagg agtttcaata tcacaagata actggcttaa ttggttaaca atataatcat
    37261 gtgtttgaaa ttcttcaaaa gataactcag gatattggtg taaataacgt ctgagttgaa
    37321 ttgttttatt ttctttatta tttgctagtt gaaaccaatc taacacccta atcactactt
    37381 tctaaaatat tgtttatagt ataacatttt atgaaattat cgtactaaat gattgctttg
    37441 agatatttta tctatgaatg ataaggcttt caagttatgt agaattactg tatgataaag
    37501 gtattaccaa acaatactta agggggatta tatactgtgg ttcaatcatt acatgagttt
    37561 ttagaggaaa atataaatta tctaaaagaa aatggtttgt ataatgaaat agatacaatt
    37621 gaaggtgcaa acggaccaga aatcaaaatc aatgggaaat catacattaa cttatcttca
    37681 aataattatt taggactagc aacaaatgaa gatttgaaat cagctgcaaa agcagctatt
    37741 gatacacatg gtgtaggtgc aggcgctgtt cgtacaatca atggtacatt agatttacac
    37801 gacgaattag aagaaacact agcaaaattt aaaggaacag aagctgcaat agcttatcaa
    37861 tcaggattta attgtaatat ggctgctatt tcggctgtta tgaataaaaa tgatgctatt
    37921 ttatcagatg agcttaatca tgcatcaatt attgacggat gtcgcttatc aaaagctaaa
    37981 attattcgag ttaaccattc agacatggat gatttacgtg cgaaagcaaa agaagcagtt
    38041 gaatcaggtc aatacaataa agtgatgtat atcactgatg gcgtttttag tatggatggt
    38101 gatgtggcta aattacctga aattgtagaa attgcagaag aatttggttt attaacttat
    38161 gttgacgacg ctcatggttc aggtgttatg ggcaaaggcg ctggtacggt taaacatttt
    38221 ggtttacaag ataaaatcga tttccaaatt ggtacgcttt ctaaagcaat tggtgtcgtt
    38281 ggcggttatg tagcaggtac aaaagagtta atagattggt taaaagcaca atcacgacca
    38341 ttcttattct ctacatcatt agcacctggg gatactaaag caatcactga agcagttaaa
    38401 aagttaatgg cttcaactga attacatgat aaattatggg acaatgcaca atatttaaaa
    38461 aacggattgt caaaattagg atatgataca ggagagtcag aaactccgat tacaccagta
    38521 attattggtg aagaaaaaac aactcaagaa tttagtaagc gtttaaaaga cgaaggtgtc
    38581 tatgtgaaat ctatcgtttt cccaacagtg ccaagaggta caggacgtgt aagaaatatg
    38641 cctacagctg cacatacaaa agacatgtta gacgaagcaa ttgcggctta tgaaaaagta
    38701 ggaaaagaaa tgaatttgat ttaatttaat atatatttat tcccacggca aatatcgtcg
    38761 tgggtttttt attttataaa tgcttagttt attaacagta agttcttata acaatgttta
    38821 gtgttgtcaa ccattaaagt ttgaatttta aaagcatctt gtagaattta gttgtatttt
    38881 tacgattaaa ttcattttga ttatttttga taataagcat tttaatagta atacatgttt
    38941 atagtgtgta gcatatgtct atactagtag taactatata gagaaagtag gaataaacta
    39001 tgtcacaaga tgtaaataaa ttaagtaagc aaccaacgcc agataaagca gaagataacg
    39061 catttttccc atcaccatat tcccttagtc aatatacagc acctaaaaca gattttgatg
    39121 gtgttgaaca caaaggtgcc tataaagatg gtaaatggaa agtattgatg attgctgctg
    39181 aagagagata tgtattattg gaaaatggaa aaatgttctc tacgggtaat catcctgttg
    39241 aaatgttatt acctttacat catttaatgg aagcaggttt tgacgttgat gttgcgacat
    39301 tatctggtta tccagctaaa ttagaattat gggctatgcc aactgaagac gaggcagtta
    39361 taagcactta taataaattg aaagaaaaat taaaacagcc aaaaaaatta gcagatgtga
    39421 ttaaaaatga attaggacct gattcagact atttatctgt ctttatccca ggcgggcatg
    39481 ctgcagttgt tggtatttct gaaagtgagg acgttcaaca aacattagat tgggcattag
    39541 aaaatgaccg ctttatagtt acattatgtc atggaccagc cgcactactt tcagcaggtc
    39601 ttaacagaga aaaatctcca ttagaaggat actctgtttg tgttttccct gactcattag
    39661 atgaaggtgc aaatattgaa ataggttatt tacctggacg tttgaaatgg ttagttgctg
    39721 atttattaac taaacaaagt ttaaaagtag ttaacgacga tatgacagga agaacgttaa
    39781 aagatcgtaa attattaaca ggtgacagtc ctttagcttc aaatgagtta ggaaaattag
    39841 cagttaatga aatgttaaat gcaatacaaa ataaataatt aaatattaat tagaggagcc
    39901 ccatatgtaa atgtatgagg gctctttttt tggcaaaatt ttagtgatac ttgtaaaata
    39961 gaacctatta tgagtatgat ttaagaaaac gcttgcaaaa ctaataactg caactagcga
    40021 tatggaggaa acatgatgtc ttatagcatt ggaattgatt atggaactgc ttcaggccgt
    40081 gtgtttttaa ttaatacaac taacggtcaa gtagtatcaa aatttgtgaa accatataca
    40141 catggtgtca ttgagggtga attaaatggt ttaaaaatac cacatacata tgcacttcaa
    40201 aatagtaatg attatttaga aattatggaa gaaggaatat catatatagt acgtgaatca
    40261 aaaatagatc cagtcaatat agtaggtatt ggtatagact ttacttcatc tactattatt
    40321 tttactgacg aaaaccttaa cccggtacat aatttaaaac aatttaaaaa caatccacat
    40381 gcgtatgtga aactttggaa acatcatggt gcatataaag aagcagagaa attatatcaa
    40441 actgctattg aaaataataa taagtggtta ggccattatg gatataatgt tagtagtgaa
    40501 tggatgattc ccaaaataat ggaagtcatg aatcgagcac cagaaattat ggaaaaaacg
    40561 gcttatatta tggaagcggg cgattggatt gtaaataaat taactaataa aaatgtacgg
    40621 tcgaattgtg gattaggttt caaagcattt tgggaagaag aaacagggtt tcattatgat
    40681 ttgtttgata aagtagaccc caaattatca aaagtaattc aagataaagt atctgcaccg
    40741 gttgttaata ttggtgaagc agtagggaaa ctggatgata aaatggcaca gaaattagga
    40801 ttatcaaaag acactatggt aagtcctttt attattgatg cccatgctag tttattaggt
    40861 attggatctg aaaaagataa agaaatgact atggtgatgg gaacaagcac atgccatctt
    40921 atgttaaatg aaaagcaaca tcaagtgcca ggtatatcag gttctgtaaa aggagcaatt
    40981 attccagaat tatttgctta tgaagcgggg caatcagcag taggtgattt gtttgagtat
    41041 gtcgctaagc aagcaccaaa gtcatatgta gatgaagcag caaatagaaa tatgactgta
    41101 tttgaattaa tgaatgaaaa gataaaacat caaatgccag gtgaaagtgg gctcattgct
    41161 cttgattggc ataatggaaa tcgaagtgta ttaagtgata gcaatttaac aggttgtatc
    41221 tttggattaa ctttacaaac taagcatgag gatatttata gagcatattt agaagctaca
    41281 gcatttggta ctaagatgat tatgcaacag tatcaagatt ggcatatgga agtagaaaag
    41341 gtatttgcat gtggcggtat acctaaaaag aatgctgtta tgatggatat ctatacgaat
    41401 gtactgaata aaaaactaat tgttatggat agtgagtatg caccagcaat aggagcagca
    41461 atattaggtg cagtcagtgg tggcgcacat aattcaatta atgatgcagt tgatgctatg
    41521 aaagagccaa ttttatacga aattaatcca gaagcggaaa aagtacaaag gtatgaaaca
    41581 ttatttaaag cttataaggc tttacatgat atccatggtt ataaaaaagc taatataatg
    41641 aaagatatcc agagtttaag agttgaggga taaaaaattt aatttgacag taattagcaa
    41701 tattaaacgc tataaattac taaaatcaat taatacagct agaatttcca tttacagaaa
    41761 tatcagcaaa atgtacatgt catatataca agaaagcgct tttatattat aattattatg
    41821 aaaatgaata ttatgtcctc ttgtgatgga ctatggttat atagaagaga caaaggagat
    41881 atttctatga aaaaaattat gattactggt gcattaggac aaattggtac agaattagtt
    41941 gttaagtgca gagaaattta tgggacagat aatgttcttg ctacagatat tagggaacct
    42001 gaagcagact cacctgtaca aaatggacca tttgaaatct tagacgtaac agatcgtgac
    42061 cgtatgtttg agttagttag ggactttgaa gcggatagtc taatgcatat ggcagcatta
    42121 ttatcagcaa ctgctgagaa aaatccaatt cttgcttggg atttaaatat gggtggatta
    42181 atgaatgcat tagaagctgc aagaacttat aatttgcact ttttcacacc aagttcaatt
    42241 ggtgcatttg gagactcaac tcctaaagtt aatacgccac aagtaacgat tcagcaacct
    42301 acgacaatgt atggtgtaaa taaagtagct ggcgaattgt tgtgtcaata ttatttcaca
    42361 cgatttggtg tagatacaag aagtgttaga ttccctggtt taatctcgca tgttaaagaa
    42421 ccgggtggtg gcacaacgga ctacgcagtt gaaatttact tcaaggctgt aagagaagga
    42481 cattatacaa gcttcataga taaagggaca tatatggata tgatgtatat ggatgatgcg
    42541 attgatgcaa ttattaaact tatggaagca gacgatgcga aattagaaac aaggaatggt
    42601 tataatttaa gtgcaatgag ctttgatcca gaaatggtta aagaagcaat acaagaatat
    42661 tacccagatt tcaaattaga atatgatgtt gaccctatta gacaaggtat cgctaatagt
    42721 tggccggatt ctattgatac aagctgttca cgtggcgaat ggggatttga tcctaaatat
    42781 gatttagtga gcatgactaa attaatgtta gaagctattg aacaaaaaga tactgttaaa
    42841 aataataact aatcatttcc attcacttta atacacggaa tgatatttta aattactctt
    42901 tattttaata aactagtgca tgaattctaa tattattaat tatacatatt gaattcgcga
    42961 gcttagttca tttttaaata aagagttttt tgtattaaaa agatataatt caaacacaga
    43021 ttacttgtct ctatgaaaat aatacgttta tactaaaaga tataggcttt ctgaaaaagt
    43081 gttaattaat ttcgaatttt tagaaaattt atcgaacagt aaaataggga ttgttataat
    43141 ttatgttaaa caaattctta tactatatta ttaggaggca atcaccatgt cacaagcagt
    43201 taaaattgaa ttacgcgaaa cattaaaaga aaaaccagat acatctcaat taggatttgg
    43261 taagtatttt acagattata tgttgagtta tgattatgat gcggataaag gatggcatga
    43321 tttgaagatt gtaccttatg gtccaattga aatttcgcca gcagcacaag gtgttcacta
    43381 tggacaatca gtgttcgaag gtttaaaagc atataaaaaa gatggagagg ttgcactttt
    43441 ccgtccagat gaaaacttta agcgtcttaa taactcgtta gcacgcttag aaatgcctca
    43501 agtcaatgaa ggtgaattgc tagaaggact taagcaatta gttgatttag agagagaatg
    43561 ggttccggaa ggtgaaggtc aatcattata tattcgtcct tttgtttttg cgacagaagg
    43621 ggtacttggc gttggtgcat cacatcagta taaattatta attattttat ctccttcagg
    43681 tgcatattat ggtggtgaaa ctttaaaacc aactaaaatc tatgtagaag atgaatatgt
    43741 acgtgctgtt cgtggcggtg taggctttgc gaaagttgca ggtaactatg cggcaagttt
    43801 attagcacaa actaatgcaa ataaattagg ttatgaccaa gtattatggc ttgatggtgt
    43861 tgaacagaaa tatatcgaag aagttggtag catgaacatt ttcttcgttg aaaatggcaa
    43921 agtaattaca ccagagttga atggcagtat tctacctggt attacacgta aatctattat
    43981 cgaattagct aaaaacttag gatatgaagt cgaagagcgc cgcgtttcaa tcgatgaatt
    44041 attcgaatca tatgataaag gtgagttaac agaagtattt ggtagtggta ctgcagcagt
    44101 tatttcacct gtaggtacat tgagatatga agatcgtgaa atcgttatta ataataatga
    44161 gactggtgaa attactcaaa aattatacga cgtctatact ggtattcaaa atggtacttt
    44221 agaagataaa aatggttgga gagtcgttgt accaaaatat taataaaaat tgaatatgat
    44281 catgaaaaca aaatgtaaaa gttaatcatg actaaaatcc ctttcaatga agcgaagcat
    44341 ggtaataaat taagtttaca atgtttattg ttaagtattg aaggggattt cacttattat
    44401 tatatttaat tcaatattta aaaagaacaa tactacgagt tcattttcag gggaataacg
    44461 aacatcatag aatcagcata atacgaaata aataaatagg agtattgata ttaatggaat
    44521 ggatattatt tgataaagat ggtacgttaa ttgaatttga tagaagttgg gaaaaaatag
    44581 gggtacgatt tgtacaatca ttgcttgaga cttttccagt acataataaa gaagctgctt
    44641 taagacaact cggtgtcatt aaagaatcta ttgatccaaa atcagtgatg ggttcaggat
    44701 ctttacaaca aattattcag gcatttaatg atgtgacggg acaagataca accgactggt
    44761 ccaagtcaac aagtcaaaag ttggtagatg aacgtattcc tgaaattaat tgggtagaag
    44821 gtgttaaaga agcacttatc gatttgaaag caaaaggcta tcaactaggt attgttacga
    44881 gtgatactaa aaaaggtgta gaacaatttt tagcacatac caatgctacc tcgttgttcg
    44941 atttgatcat ttctaccgaa gcggatgcct atgagaagcc aaatcctaaa gtattatcgc
    45001 ctttatttga gcaatataat gtagatcctc agaaagtagc tatagtagga gacactgcta
    45061 atgatatgaa aaccgctagt aatgcaaatt taggtatggc aataggtgta ttaacaggta
    45121 ttgcaacaaa agaagaatta catgaagctg atattatttt aaatagtgcg gcagatattt
    45181 tagaagcttt aatttaaaaa gtaagacata gtgtatatgt gaatcaatgt aaccaataga
    45241 attgataatg taatcgatga tatctatgga atatacattg aatataaaca tatacgctat
    45301 gtctttagtc ttttatcgtg tatctacttg tcgatatgtt tgaataattc gagcaatttt
    45361 gtctatcata ggatttaaag attcggggtc cttatggata tcatattcat taatattgat
    45421 acgtaccaca ggacatgcat taaagctatt aatccaatcg tcatagcgtt taaatagctt
    45481 tttccagtat tcagggtctg tattaatttc catttcgcga ccgcgttcaa taatacgatc
    45541 aatgacctca tcatagttac attctaaata aatcattaca tcaggtttag gaaaataagg
    45601 tgtcatgacc atggcattaa ataagtctga atatgttttg aaatcctctt tactcattgt
    45661 gccttcttct tcatgcattt ttgcaaaaat atcaacatct tcataaattg atcgatcttg
    45721 gacaaagcca ccaccatatt caaacatacg cttttgttct ttaaagcgtt cagctaagaa
    45781 ataaatttgt aaatggaaac tccaacgttc aaaatcgctg taaaatttat ctaaatatgg
    45841 attatgttcg acattttcaa aagacgtttt aaagtttaat ttatctgcaa gtgcttgcgt
    45901 tagtgttgat ttcccaacac caactgtacc tgcaatggtt ataatggcat tttgtggaat
    45961 accgtaatta ttcattggta atatctccta tcataggtaa tataatatgt aatatatctt
    46021 cgtaatcttg ttcatttttt agaaaatcaa tagaagttgt atcgattaaa actacatttg
    46081 aaccattact ttgtaaggac tcataatact cacgataatc tttttttaac tttaacagat
    46141 attcatcttc tatttgatgc tcaaaactac ggttacgttt agcaattcta gattttaaca
    46201 catcaaggtc tgcatctaaa aagataatca tattcggcat aatcatatct tcagttaaaa
    46261 tatcataaat tttactgaat ttctgaaatt caacagaact caaagtattt ttagcaaata
    46321 tcttattttt atgtatatga taatcactaa ctacaccttg atttagttgt gttacatctt
    46381 gaaattgctt atatctattg cataaaaaga acatttcagt ttgaaaactc catttagaga
    46441 tatcttcata aaagtctgat aaaaatggat tttctgtgat gatttctttt tcttcataaa
    46501 aatctaaagt ttgacttaat ttgtgtgcaa gtgaagattt acctacgcca ataggacctt
    46561 caattgctat aaaaggtttg ttcatattca catctccaaa tgttaaaata gacattgagt
    46621 attgtaccat aagtgaggta tctggacata aatgacaaat gatatatatt ttatgacatt
    46681 agcgattgaa gaagctaaaa aggcagctca actaggcgaa gtacctatag gtgctatcat
    46741 cactaaagat gatgaagtta tcgctagagc acataattta agagaaacac tacaacaacc
    46801 aacggcgcat gctgaacata ttgcaattga acgtgcagcc aaagtgttag gtagttggcg
    46861 tttagaagat tgcacattat atgtaacctt agaaccatgt gtcatgtgcg caggaacaat
    46921 tgtaatgagt cgcattccaa gagtcgtcta tggcgcagat gatcctaaag gtggttgtag
    46981 tggcagttta atgaatttat tgcaacaatc taattttaat catcgtgcaa ttgttgataa
    47041 aggtgtactt aaagaagcat gtagcacatt attaacaaca ttttttaaaa acttaagagc
    47101 caataagaaa tccaccaatt agacgatagt acatactaat taaaatttgt tagaattaca
    47161 ttatatgata aattaatgac aatataaatg ttaatagaaa agtatgtgca ctgagtatat
    47221 attcttatgt aagaacaaga ttttaaattt acgaaacgag gtaacataat gataaaacta
    47281 atagccactg atatggatgg cacgctactt aatgcagcac atgaaatttc tcaacctaat
    47341 attgatgcga ttaaatacgc tcaagaacaa gggataacgg ttgttatcgc gacaggtcga
    47401 gcattttatg aagcacaagc accagttgct gacacagatt taacagtacc atatatttgt
    47461 ttgaatggtg ctgaagtacg tgatgaaact ttcaatgtaa tgagcacttc acaccttaat
    47521 aaatcgttag tacacaaaat tacaaatgtt ttaaaagatg caggtattta ttatcaagta
    47581 tacacgagtc gtgcgattta tactgaagat ccacaaagag atttagacat ttacatagat
    47641 attgctgagc gtgcaggtca acacgcaaac gttgagcgta ttaaaaatgg tattcaaaga
    47701 cgcatagata atggtacgtt gaaagttgtt gataattatg atgctattga aaacatacct
    47761 ggtgaattaa ttatgaaaat attagcattt gatggaaatt tagaaaaaat tgacaaagct
    47821 agtaaaattt tagctgaatc tccgaattta gctatatcat catcttcgag aggaaatata
    47881 gaaataacgc attcagatgc acaaaaaggc attgcgctag aaacaattgc cgaaagatta
    47941 gggattgaaa tgaaagatgt catggcaata ggtgacaatt taaatgacgt atcaatgtta
    48001 gagaaagttg gctatccagt tgcgatggaa aatggtgctg aagaagttaa aaaaatagcg
    48061 aaatatgtca cagatacgaa tgaaaatagt ggtgttggaa aagctattat gaaattatta
    48121 cgtgaacaac aagtttaata aaaaagaggg gtcaaatatg aaaggattaa ttattattgg
    48181 cagtgcacaa gtgaattcac atacaagtgc actagcaaga tacttaactg agcattttaa
    48241 aacacatgat attgaagcgg aaatattcga tttagcagaa aaaccgttaa atcaattaga
    48301 tttttcagga acaacaccgt ctattgatga aatcaaacaa aatatgaaag atttaaaaga
    48361 gaaagcaatg gcggcggact ttttaatatt aggaacgcca aactatcatg gttcatattc
    48421 tggaatattg aaaaatgcat tagatcatct aaatatggat tattttaaaa tgaaacctgt
    48481 aggcttaata ggaaatagtg gtggaattgt tagttcagag ccattgtcac atttaagagt
    48541 aatcgtcaga agtttactag gcattgcagt accaactcaa atagcaacac atgattctga
    48601 ttttgctaaa aatgaagatg gttcatatta cttaaatgat agtgaattcc aattacgagc
    48661 aagattattt gtcgatcaaa ttgtatcttt tgtgaataat agcccatatg aacatttaaa
    48721 ataaattaaa aaatatgtaa atatacatta aaaataggta tgtagcttaa ggtatttcat
    48781 tgagaatgct tttaagtcac atgcctattt ttttgttcta atgcaccaat agtaagcctt
    48841 tgagtttact attgtgagca atgttattaa
//
LOCUS       DATTQF010000007        46313 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.007, whole genome shotgun sequence.
ACCESSION   DATTQF010000007 DATTQF010000000
VERSION     DATTQF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 46313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.007"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..658
                     /locus_tag="VB241_000293"
     CDS             <1..658
                     /locus_tag="VB241_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HEP1243626.1"
                     /translation="LNKLLLGLHSVNTTTALSEMPIGNSIADFILLNGKGVVYEIKTE
                     LDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVLTSRNTLSEVQK
                     PKENNSLLNSKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFDVFKEIPMTKLH
                     NNMIFELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHFLKDTNNPPHFT
                     "
     gene            complement(863..2455)
                     /locus_tag="VB241_000294"
     CDS             complement(863..2455)
                     /locus_tag="VB241_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1243627.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTIVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            2412..3890
                     /locus_tag="VB241_000295"
     CDS             2412..3890
                     /locus_tag="VB241_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243628.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VISIITYLESFKSLYHKAFYKFVLSVLSLPLFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKTQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     IIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFSILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNDVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            3896..5059
                     /locus_tag="VB241_000296"
     CDS             3896..5059
                     /locus_tag="VB241_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243629.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVVFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVTP
                     DVAEWGNILRTGSNYLETHSNLAIVPGICIMFVVLAFNFIGDAVRDALDPRIH"
     gene            5076..6851
                     /locus_tag="VB241_000297"
     CDS             5076..6851
                     /locus_tag="VB241_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1243630.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDNSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            6889..8895
                     /gene="ggt"
                     /locus_tag="VB241_000298"
     CDS             6889..8895
                     /gene="ggt"
                     /locus_tag="VB241_000298"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEP1243631.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLQSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSKL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVTGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLPYRQMYGSNVY
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIT
                     WLARKKGYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(9432..10058)
                     /locus_tag="VB241_000299"
     CDS             complement(9432..10058)
                     /locus_tag="VB241_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEP1243632.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPEDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVLVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            10267..10845
                     /locus_tag="VB241_000300"
     CDS             10267..10845
                     /locus_tag="VB241_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEP1243633.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKLEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            11228..12325
                     /gene="ugpC"
                     /locus_tag="VB241_000301"
     CDS             11228..12325
                     /gene="ugpC"
                     /locus_tag="VB241_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP1243634.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            12338..13609
                     /locus_tag="VB241_000302"
     CDS             12338..13609
                     /locus_tag="VB241_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP1243635.1"
                     /translation="MSKILKYITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            13612..14880
                     /locus_tag="VB241_000303"
     CDS             13612..14880
                     /locus_tag="VB241_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1243636.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     SKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            14882..15721
                     /locus_tag="VB241_000304"
     CDS             14882..15721
                     /locus_tag="VB241_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP1243637.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            15898..16974
                     /locus_tag="VB241_000305"
     CDS             15898..16974
                     /locus_tag="VB241_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP1243638.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAACA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLYKSAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            16999..18039
                     /locus_tag="VB241_000306"
     CDS             16999..18039
                     /locus_tag="VB241_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP1243639.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNCKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            18094..19062
                     /locus_tag="VB241_000307"
     CDS             18094..19062
                     /locus_tag="VB241_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEP1243640.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPTEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(19421..19915)
                     /locus_tag="VB241_000308"
     CDS             complement(19421..19915)
                     /locus_tag="VB241_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP1243641.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            20148..21527
                     /gene="uhpT"
                     /locus_tag="VB241_000309"
     CDS             20148..21527
                     /gene="uhpT"
                     /locus_tag="VB241_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HEP1243642.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(21886..22644)
                     /locus_tag="VB241_000310"
     CDS             complement(21886..22644)
                     /locus_tag="VB241_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1243643.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(22637..24193)
                     /locus_tag="VB241_000311"
     CDS             complement(22637..24193)
                     /locus_tag="VB241_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1243644.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYIANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(24190..25158)
                     /locus_tag="VB241_000312"
     CDS             complement(24190..25158)
                     /locus_tag="VB241_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP1243645.1"
                     /translation="MKSKIYILLLFLIFLSACTNTRHSESDKNVLTVYSPYQSNLIRP
                     ILNDFEKQEHVKIEIKHGSTQVLLSNLHNEDLSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIQGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            25746..27995
                     /gene="pflB"
                     /locus_tag="VB241_000313"
     CDS             25746..27995
                     /gene="pflB"
                     /locus_tag="VB241_000313"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP1243646.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            28018..28773
                     /gene="pflA"
                     /locus_tag="VB241_000314"
     CDS             28018..28773
                     /gene="pflA"
                     /locus_tag="VB241_000314"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP1243647.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            28877..29044
                     /locus_tag="VB241_000315"
     CDS             28877..29044
                     /locus_tag="VB241_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243648.1"
                     /translation="MRQVQQWLKEDAFVSSYADKECDALLKMGFQYHRMGYYLLLAYE
                     YESFQIFIDPE"
     gene            29095..30858
                     /locus_tag="VB241_000316"
     CDS             29095..30858
                     /locus_tag="VB241_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEP1243649.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSSLFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHSENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(31022..31366)
                     /locus_tag="VB241_000317"
     CDS             complement(31022..31366)
                     /locus_tag="VB241_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HEP1243650.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSHLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            31556..33385
                     /gene="coa"
                     /locus_tag="VB241_000318"
     CDS             31556..33385
                     /gene="coa"
                     /locus_tag="VB241_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HEP1243651.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNENSKY
                     DTPIPDWYLGSILNRLGDQIYYAKELTNKYEYGEKEYKQAIDKLMTRVLGEDHYLLEK
                     KKAQYEAYKKWFEKHKSENPHSSLKKIKFDDFDLYRLTKKEYNELHQSLKEAVDEFNS
                     EVKNIQSKQKDLLPYDEATENRVTNGIYDFVCEIDTLYAAYFNHSQYGHNAKELRAKL
                     DIILGDAKDPVRITNERIRKEMMDDLNSIIDDFFMDTNMNRPLNITKFNPNIHDYTNK
                     PENRDNFDKLVKETREAIANADESWKTRTVKNYGESETKSPVVKEEKKVEEPQLPKVG
                     NQQEDKITVGTTEEAPLPIAQPLVKIPQGTIQGEIVKGPEYLTMENKTLQGEIVQGPD
                     FPTMEQNRPSLSDNYTQPTTPNPILKGIEGNSTKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQNKPSETNAYNV
                     TTHANGQVSYGARPTQNKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(33567..33674)
                     /locus_tag="VB241_000319"
     CDS             complement(33567..33674)
                     /locus_tag="VB241_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243652.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVCHIDVRGLF"
     gene            complement(33972..35156)
                     /locus_tag="VB241_000320"
     CDS             complement(33972..35156)
                     /locus_tag="VB241_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEP1243653.1"
                     /translation="MQEAYIVAYGRSATAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNVGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(35186..37447)
                     /locus_tag="VB241_000321"
     CDS             complement(35186..37447)
                     /locus_tag="VB241_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEP1243654.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     LVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLYENGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHNDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENEHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLSDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRHGHFISDHDYHIALNIATILSGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(37634..38845)
                     /locus_tag="VB241_000322"
     CDS             complement(37634..38845)
                     /locus_tag="VB241_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEP1243655.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(38957..40462)
                     /locus_tag="VB241_000323"
     CDS             complement(38957..40462)
                     /locus_tag="VB241_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEP1243656.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(40488..42065)
                     /locus_tag="VB241_000324"
     CDS             complement(40488..42065)
                     /locus_tag="VB241_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HEP1243657.1"
                     /translation="MEVTCLKQITWHDLQHIIKDDDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGVAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     IDDETYLHYTFPSVDVALLRGTYADQQGNIYLTQESYLSECYHVALNAKANHGKVIVQ
                     VKDLVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGELKQSIHKV"
     gene            42424..43095
                     /locus_tag="VB241_000325"
     CDS             42424..43095
                     /locus_tag="VB241_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP1243658.1"
                     /translation="MRVNVLQHTPNEGVGYIGEWAKERGHEVYIYHPYMNDGVLPLAE
                     ETDMLVILGGPMNPDDEMPWIQSERQLILVLMAQNKPIYGACFGAQQISKALGYAVLK
                     SPVKEVGWDDVYLESEIIPNIPSRLKALHWHEDMFQIPQQAELLFSGNHIRNQGFIID
                     HHVIGLQFHFEPGPDDVREIVLNDAQYINHSILEQSAEDILAFEVPKQNRQAIFRILD
                     FITQA"
     gene            43319..44461
                     /locus_tag="VB241_000326"
     CDS             43319..44461
                     /locus_tag="VB241_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP1243659.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTI
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(44772..46247)
                     /locus_tag="VB241_000327"
     CDS             complement(44772..46247)
                     /locus_tag="VB241_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1243660.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTSSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSLFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEDYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNNNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
BASE COUNT        15331 a         7508 c         8531 g        14943 t
ORIGIN      
        1 gcttaataaa ctccttttag gactacattc tgttaataca actactgcac tttctgagat
       61 gcctatagga aatagtattg ctgattttat attgttaaat ggtaaaggcg ttgtctacga
      121 gattaaaaca gaattagata agttggatag attggataat caaattaatg attattatga
      181 agtatttaat tatgtagtag tcattacaaa tgacaaacac ttgaataaag ttatggctag
      241 atacaaagat acaacagtcg gaattttagt attaacatct agaaatacac tgagtgaagt
      301 tcaaaaacca aaagaaaaca atagtctctt aaactcaaaa gcgatgtaca actttttacg
      361 aaaagaagaa agaaaaagag ttattgcaca aaatcatatg gatgtgccaa cttataatga
      421 tttcacagag tatgatgtgt tatttgacgt gtttaaagaa ataccaatga cgaaactgca
      481 taacaatatg atttttgagt tgaaaaaaag aggcaacatg aaagaataca aagatgaatt
      541 tttagcagct ccgactgaaa ttaagttctt gttatatttc gcaaaaatga caaagaaaga
      601 taaaaataaa ctatatcatt ttcttaagga tacaaataat cccccgcact tcacatagtg
      661 gcgggggatt tatcatgcgt taaaatgccc aaagtatggg tgtgaccctt aaatcatggc
      721 aaagcatcta acacttgata tggtgccgag ccctaacata gcacaatata gttagtatga
      781 caaagtcatc taacataaca catcagatgt tgcccgattt attccagacc atacagtgat
      841 aaagttgcac aacgcatgac ttttatttag caataactgc tacttctgaa ataagttgct
      901 ttgcatagtc tgattgcgga tgtttgataa tatcttctgt gttattaagt tcaacgattt
      961 cgccattttt cataactgca actcgatcac atatttcatt gataacaccc atgtcatgtg
     1021 tgatgaataa ataagtgatg ccgaagtcta actgtaattg ttttaataac tcgatgatat
     1081 ctttttgaat tgaaacgtct aaagctgata ctgcctcgtc gcaaacaatc actttaggtt
     1141 ctacagcaag tgctcttgcg atacttacac gctggcgttg cccaccagat aattcgtgtg
     1201 gatagcgata taagaaactt tgatctaggc caactttttc taacaacgat acgatagttt
     1261 taataatgtc gtcattatct ttgactttcc catgaatgat tagtggtcgt ttaatcacat
     1321 caatgacttt aaatcttgga ttaatagatg cgaatggatc ttggaaaatc atttgtatct
     1381 cttgtcgtaa agatttcaat tcatcatctt taaataaact taatggtaat tcgttatacc
     1441 aaataaagcc ttctgacact tcctttagac cgacgaccgt cttagctaat gtcgatttcc
     1501 ctgaccctga ttcaccgaca atgcctaacg tttcgccttt tctaatagcc aagttaatat
     1561 cattaactgc tcggtatagg ctgccactcg gtgatgtgta atccacgctc acgcgatcga
     1621 attttaataa aatatcattg tttaacggtc ttggcggacg cgtttgatga atatcaggaa
     1681 tcgcatctat taagcgtttc gtataggtat gttgtggcga tttaaaaata ctttcaaccg
     1741 tgccactttc aacgacactt ccatctttca ttacaatcac atcgtcgcaa aattgataca
     1801 cagcacctaa atcgtgagtg ataaaaataa tagacgtttc tgtgtactca taaagggact
     1861 tcattaactg cagtaattga ttttgtgtac tggcatctaa tgccgttgtt ggttcatctg
     1921 cgattaaaat ttgtggcttt aaaatcaatg ccattgctat catgacacgt tgacgcatac
     1981 caccagaaag ttcatgcgga taagcatcaa attgtcgagt tgcatgtttt atacctactt
     2041 tttctaaaat gtctattgtc atcgactttg cttcagattt agatacacgt ttatgttgaa
     2101 atagtacttc tgtaatttgt ttgccaatcg ttaatcttgg attcaacgaa gagagtggat
     2161 cttgaaaaat cattgaaata tccttgcctc gaatttgttg taacgctgaa gttgataaat
     2221 tagttaacga ttgcccatta aaaataattt ctcctgttaa cgtgtgatcc ggataatctg
     2281 gtagtagccc taaaatagat ttagcggtaa tactttttcc tgatcctgat tcaccaacaa
     2341 tacctaggat atgttttttt cgtaattcga aagagacgtt ttttaccgct tgaactgtag
     2401 tttcatcata attgaattgt acattcagac tgttgacttc taataaattt gacatgatgt
     2461 tgtgcctccc tattcacaaa tgtgtaaatt tattctatac ttttgtatat catagtaatc
     2521 tactcaagat tattttacaa ggagtgatta gcataattac ttatttagaa tcttttaagt
     2581 cgttgtatca caaggcattt tacaagtttg tattatcggt attaagcttg ccattatttt
     2641 tatatgcagt gataaagttt ttcttttctg ctaagagaaa aaatttttac gctaataatt
     2701 ctgaaatttc agaaatcgaa caggcgttac atcaaaaata taaatattta tcgcagcaaa
     2761 agtcatccac acaaatacat aaagaagcat taaaaatatt caagacacaa agttctaata
     2821 cgaattcaaa gaatattgaa caagcacatt tttcaacata ctttgaaaat gtattatttc
     2881 ataagttcat cattatcaaa gtgatattgg cgttaccgat gttcatctta ttgactattt
     2941 atttacagcc attagttaga tatatttttg agcgaattgt catggctgtg attgtcatca
     3001 ttggtgttat tgtcagtgtg tttagcattc tgtatttttc accgcttgat gcggcttata
     3061 gcatactggg acaaaatgca acaaaggcac agatacatca attcaatgta ttacatcatc
     3121 ttaacgaacc ttattttatt caattgtggg ataccatcaa gggtgttttt acctttgact
     3181 taggtacgac ttacaaaggg aatgatgttg tgactaaagc agttggcgaa agaattccaa
     3241 ttacaataat tgtcgcagta ttagcgctaa ttgtggcatt aattattgca ataccaattg
     3301 gtattatcag tgcgatgaag cgaaatagtt ggcttgatat cacgttaatg ataattgcat
     3361 taattggttt gtctattcca agtttctggc aagggttatt attcatttta gcgttctcat
     3421 tgaaattgga tattttgcca ccatcttata tgccagaaca tccaatatcg ttgattttac
     3481 ctgtacttgt cattggaaca agtattgctg cttctatcac gcgtatgaca aggtcttctg
     3541 tacttgaagt aatgcgcagc gattatgttt taactgctta tgcaaaagga ttatcgacga
     3601 cacaagtggt tattaaacat attttgaaaa atgccattat tccaattgta acgctagttg
     3661 gtcttctagt ggcagagtta ctaggcggtt cagcagtgac ggagcaagta tttaacatta
     3721 atggtatcgg tcgttatatc gtccaaaaac aactaatacc tgatatccca gcagtcatgg
     3781 gtggggtcgt atatatatca attgtaatat ctttagcaaa cttaattatt gatatatttt
     3841 atgctttaat cgatccaaaa ttacgtagtg aaattaacga aaggaagtga ggcatatggt
     3901 aaaacttaca acaaagatag cttccttaaa actattcgca agttatgcta ttgcaactta
     3961 tattttagtt atattaacga gtgcattaaa tctttttaaa ggttatgtgg ccgatacgtt
     4021 ctatattgct gaaacattgc taatcgtttt aaccatcatt ttaattatca tcttaacaac
     4081 ggaacaaaca tggaagcatc atgacctatg gcgacgtatc gtcgaagtgt tgttattgtt
     4141 gatgacatta acagggaacg tatttacatt attaatgttt gtaagtatta gacgttacca
     4201 acgtacatcg caaatacata gttataatgg ctgggaatcg tttatacgaa aaactactag
     4261 acatcgtatt gcgattatcg ggttacttat tttagtctat atgctgacat tatcaattgt
     4321 gtcacaattt acatttgata cgacattggc tactaaaaat cagttcaacg cactgttaca
     4381 tggaccgagt ctagcctatc cgtttggtac tgatgatttc ggtagagact tatttacacg
     4441 cgtagttgtc ggaacgaagc tgacattttc aatttcaatt atttcagtag ttattgcagt
     4501 tgtttttggt gtgttactag gcactatcgc aggttatttt aatcatattg ataatttaat
     4561 aatgcgaatt ttagatgtag tgtttgcaat tccatcatta ttgttagcgg tggcaattat
     4621 tgcatcgttt ggagcaagta ttccaaattt aattattgct ttaagtatcg gtaatatacc
     4681 atcatttgca cggacaatgc gtgccagtgt tttagaaatt aaacgcatgg aatatgtaga
     4741 tgcagcacgt atcactggtg aaaacacttg gaatatcata tggcgttata ttttgccgaa
     4801 tgcgattgcg cctatgattg tacgtttttc attaaatata ggtgtggttg tattaacaac
     4861 aagtagttta agtttcctag gacttggtgt tacacctgat gtagctgaat ggggcaacat
     4921 tttacgtacc ggtagtaact acttggaaac gcacagtaat ttagctattg tacctggtat
     4981 ttgtattatg ttcgtcgttt tagcatttaa ttttataggt gatgcagtgc gtgatgcgct
     5041 agatccaaga attcattaaa aaggtaggga tagatgtgaa gaaaatcatt agtatcgcaa
     5101 ttatagtttt agcgttggta ttaagtggtt gtggtgtccc tacgaaatca gaagtggctc
     5161 aaaagtcatc gaaagttgaa gtgaaaggcg agcgaccaac gatacatttc ctaggacaag
     5221 caagttatga aaatgatatg aatatcgtca aagatcaatt ggaaaatgca ggatttaacg
     5281 tgaagatgaa tatccaacca gattatggta gctatcgtac acaacgtcaa gccggcaatt
     5341 atgatatcca aatcgatgac tggatgacag tattcggtga cccgaactat gctatgacgg
     5401 cattatttag ttctacagga tcaaatagtt tattgaaaga taaacatgta gaccagttgt
     5461 taaataaagc ttctactcaa aatgaagcag atgttaaaca aacatataag caaattgaag
     5521 atgaagttgt atttgataaa gggtatatgg cgcctttata tggatcaaaa aagaatttag
     5581 tatatgacaa taaagtgtta gataaaaata gtgttggatt gccaaattca cgtgcattaa
     5641 tatggcaaca atttgattac aacaatagta gagaacgaga tacgcggcca cttgtgatga
     5701 cacaacaaga cggtgaaatt cctacattgg atccaatacg ttcaattgcg ccgtcagtat
     5761 attcaattaa tatgaatatg tacacaaggt tattattatt agatgaaaat gaccacttaa
     5821 caacgaaagg ttcgttaagt cgtgattatg ctgtgaataa ggacaataaa gcgttttatt
     5881 tcttattaag agatgatgat tattttgcga aagtggttaa tggacaagca cgtaatactg
     5941 gagagcgtgt atcggctgaa gatgttaagt tttctttaga tagagcacgt gataaaaagt
     6001 ctgtgcctaa caataatact tacaatatgc acaaacatat aaatgacatc aagatattaa
     6061 aagatgagga catcgatcag ttgcgtaaag agaaggacaa ggacgataat tccatctatg
     6121 ataagttgat taaagcgtat aacgtcaaat cgttaacgac agatggtcaa aaagtaaata
     6181 ataaagacgg tatttatcaa attgttaaaa ttacgacaga tcaatcgatg cctcgagagg
     6241 taaattactt aacacactct tcggcaggca ttttatctaa aaaatttgtt aatcaagtaa
     6301 atcaagaata tccaaaagga tatggggata gcagtaccat tcctgcaaat tcagatggaa
     6361 aaaatgcgct ttatgcaagt ggcgcataca ttatgacaca gaaaaatgca tatcaagcaa
     6421 cgtttcaacg taatccagga ttcaacgaaa cagaaaaagg tagttatgga ccagctaaaa
     6481 ttaaaaatat tacattgaag tttaatggtg acccgaataa tgcattgtca gaacttagaa
     6541 atcattcaat tgatatgttg gcagatgtga atcaaaaaca ttttgattta attaagtcgg
     6601 ataaaaattt aagcattatt cgcaaaaatg gacgtaagtc agtcttttta atgctaaata
     6661 ttaaaaaagg tatatttaag acgcatccaa acttgagaca agcagtagtt aatgcgatag
     6721 atcaggatca atttattaag ttttatcgtg gcgataaatt taaaattgca tcaccgatta
     6781 caccacttgt cgatactggt aacgagcaac gtcaagattt agaaaaagta gaaaaagcca
     6841 ttaatcaata atgttttaaa tatttaacag aaagtaggag gatatagtat ggtcattaac
     6901 ttaaatgaca aacagacaaa aacatctaaa gaagggttaa tttccgtatc acatcctctt
     6961 gcggctaaaa ttggtaagga tgtattagat caaggtggca acgctatgga tgccgtgatt
     7021 gcaattcaac tggcattgaa tgtggtagaa ccatttgcat caggtattgg tggtggcggg
     7081 tacttgctat attatgagca aagtactggc agtataactg cgtttgatgc gcgtgagaca
     7141 gcccctgcac atgtagacaa acaattttat ctagatgatt caggcgaata taaatcattt
     7201 tttgatatga ctacacatgg taaaactgtc gctgtgccag caattccaaa gctgtttgat
     7261 tatattcaca agcgttatgc taaattgtca ttggaagatt taattaatcc tgcaattgaa
     7321 ctagccattg aaggtcattc agctaattgg gctactgaaa aatattcgcg ccagcaacac
     7381 gcgcgattga caaagtatca cgaaacggca caagtattta cccatgaaaa tcaatattgg
     7441 cgtgaaggtg attggattgt acaacccgaa ttaggtaaga catttcaaat attaagagaa
     7501 caagggttta atgcatttta taaaggtgac attgcgaaac aattagtcaa tgttgtcaaa
     7561 gcatgtggtg ggacaatcac tttagaggat ctagccaatt atgatattca gattaaagcg
     7621 ccaatcagtg cgacatttaa agactatgac atttattcaa tgggaccatc tagttctggc
     7681 ggtatcacgg taattcaaat attgaagtta ttagaacatg tcgatttaca atctatgggt
     7741 ccaagatctg ttgattactt gcatcatttg atacaggcga tgcatttagc atatagtgat
     7801 cgcgcgcaat acttggcgga tgataatttt catgaggtgc ctgtacagtc attaattgat
     7861 gacgattatt taaaagcacg cagtaagctc attgatagca ataaagcaaa tattgatata
     7921 gagcatggtg ttgtgtctga ttgcattagt catacagatg ttgaagaaaa tcataccgaa
     7981 acaactcatt tttgtgtgat tgataaggaa ggtaatattg cttcatttac gacatcaatc
     8041 ggtatgattt acggatcggg tatcacgatt ccaggctacg gcgtgttatt gaataccaca
     8101 atggatggtt ttgatgttgt gacgggtggg atcaatgaaa ttgcacctta taaacgacca
     8161 ctaagtaaca tggctccaac gattgtgatg catcacggga agccaatatt aacagtaggt
     8221 gcacctggtg ctataagtat cattgctagt gttgcgcaaa cattaatcaa tgtattagtg
     8281 tttggcatgg atattcagca ggctatagat gaacctagaa tttatagtag ccatcctaat
     8341 cgcattgaat gggagcctca attttcacaa tctacaattt tagcattgat tgcacgtgga
     8401 catgcaatgg aacataaacc agatgcctat attggagatg tacatgggct acatgttgac
     8461 ttgaatacac gtgacgcatc gggaggtgct gatgatacga gggaaggtac agtgatgggt
     8521 ggtgaggtat tatcaattag aaaacaacca ttaccatatc gtcaaatgta tggtagtaat
     8581 gtatatcgtg tatatttcaa tgatgtgcag ttacctttat tggcagatca agtgcgatgg
     8641 atgcatgaca aatattgggt tgatgaaagt gtcgttagaa tcatcttctc tgaagttagt
     8701 gcacatattg aagatttaag aagttatgaa aacgcaggag aaaattatat agatattacc
     8761 tggttagcac gaaagaaagg ttatcaagta acattgaagg atgacggttt atacttaact
     8821 gatgatacat atacttcagt gaaacggaac acaaatgcat actatagata tgatcgagat
     8881 agtatcacaa gatagtttat acgcttgata tgaagtttgt agtggataag gacttttgtt
     8941 gaaagaaagg attcgatttc gaactttagc gtgacgcttg agtgtagtaa tgttggaaat
     9001 gctatacgaa acgctagtca cctttgtaaa tatacaacac ctcacatatt cgtttttgat
     9061 gtacatcaca atagttagtc taaaatctaa cgtgatgtgg tttggtacag ttgaacgagt
     9121 tatgtgaggt gtttttataa tgtgggattt taatagatga tttggatgtt tctataatta
     9181 cagcataagt attaatgttc gctactaagt ttaaccagaa gtttttatgt gtcttcatac
     9241 tttcatatct actctcaaaa tatatcatca acactatcat atgttttaaa aatatttaca
     9301 tcataactaa agtgaatgtc aaaatttaaa tttgtaaaaa caatagatgt atataaaaag
     9361 ttgaaaagga ttgtaagaaa atcaaaaaaa cactgtatga cgtttgatta gtcacacagt
     9421 gtagataaca attaaaatgt tgttgccaat ttttcagcat tattaatact agttgcttta
     9481 atttcttcag ttttatgcgg ttcggcattg tgtccttcaa taatgattgt ttcatatgat
     9541 ggcacaccta agaatgtcat aattgttctt aaataacggt cacccatttc aaaatcagca
     9601 gcaggtcctt cagtataata tccaccacgt gattgaatgt gtaatacttt tttgtcagtt
     9661 agtaaacctt gtggtccttc agcagaatat ttaaaagttt tacctgcaat tgaaatagca
     9721 tcaatatatg ctttaactac aggtgggaaa gaaaggttcc acataggcgt tacaagcaca
     9781 tatttatctg cacttaaaaa ttcttctaaa atgtcactca atcttgaaac tttcatttgt
     9841 tcatcatcag ttaacgtttc gccattactc attttccccc aaccagttaa tacatctttg
     9901 tcaataactg gaatataagt ttcaaataaa tcaatatgtt tcacttcatc ttcaggatgt
     9961 tgttgttgat atgtttcgat aaatgcttta ccagccgcca tagaatttga taccagttca
    10021 ttaaaagggt gtgctgtaat atataatact tttgccattt gaaaattctc ctctgtttct
    10081 gttattttct taagtataat tattatacta ggtataaaat ttaatatcaa tcaaaatatt
    10141 caaattacca tcattttctt catctatatt ttgcagtact actaaagtat gagtgcattt
    10201 aattatgaaa tagttgattt agaatatata cttaataccc aaaatatatg aaggatggat
    10261 gccactatga caaagcgacc aaaacgtatt ttggcaacaa ttatcatttt tctttcacta
    10321 ttatttacga ttatttatat agatgacata caaaaatggt ttaaccaata taccgataaa
    10381 ttgacacaaa atcataaagg acaaggacac tcaaaattgg aagacttttt tagagggagt
    10441 cggattactg agacttttgg taaatatcaa cattcaccat ttgatggtaa gcattatggt
    10501 attgattttg cattgccaaa aggtacacca attaaagcgc cgacgaatgg taaagtaaca
    10561 cgtatcttta ataatgaatt aggcggcaag gtattacaga ttgccgaaga taatggagaa
    10621 tatcaccagt ggtatctaca cttagacaaa tataatgtca aagtaggtga tcgagtcaaa
    10681 gcaggtgata ttattgcata ttcaggcaat acaggtaaac aaacgacagg cgcacattta
    10741 cattttcaaa gaatgaaggg tggcgtaggt aatgcatatg cagaagatcc taaaccgttt
    10801 atcgatcagt tacctgatgg ggaacgtagc ctatatgatt tgtagttata gaagagtgcc
    10861 cgcagtctaa aaaatcaagc aatcattgtg tgagtataat acttacataa tggttgcttt
    10921 tttcaatgaa aatcgaaatg ctaagtcata cttgtttgat ttagatatta tttaaaatgt
    10981 aagacaaggt tgttagcatt ggcagtgaaa tttcgcacgt aaaaaatcat tattgacaca
    11041 ctaaaaatag ttgtgtgcta tatcaatttt ctgtataaaa gtttaattct gacagtaatg
    11101 taaacgttta caatttatga ttgacattaa taataagtga ctatatgatt tatgtaagta
    11161 tttgtgcaaa cgttttcaca aagtgtattg cacaatcaaa ctgtaaacaa agtatgggag
    11221 gcataacatg gcagaactaa agttagagca tattaaaaag acgtatgata acaacaatac
    11281 tgtagtgaaa gattttaatc tacatattac tgacaaagaa ttcattgtat ttgttggacc
    11341 atcgggatgt ggtaaatcaa caacattacg aatggttgct ggactagagt ctatcacatc
    11401 tggagatttt tatattgatg gggaacgcat gaacgatgtt gaaccaaaga atagagatat
    11461 tgcgatggta tttcaaaact atgcattata tccacatatg actgtttttg aaaatatggc
    11521 atttgggcta aagctacgta aagtgaataa aaaagagatt gaacaaaaag ttaatgaagc
    11581 agctgaaata ttaggattaa ctgagtatct tggtcgtaaa ccaaaagcgt tatctggtgg
    11641 acagcgtcaa cgtgttgctt tgggcagagc tattgttagg gatgcgaaag tctttttaat
    11701 ggatgaacca ttatcgaatc ttgatgcgaa gcttcgagta caaatgcgca cagaaatatt
    11761 gaaattacat aagcgactta atactacgac aatttatgtt acgcatgatc aaactgaagc
    11821 attgacgatg gctagtcgaa ttgttgtttt gaaagatggc gacattatgc aagtcggcac
    11881 acctagagaa atatatgatg cccctaattg catttttgtg gcgcaattta tcggctcacc
    11941 agcaatgaat atgttgaatg ccacagttga aatggatgga ttgaaggtag gaacacacca
    12001 ttttaaatta cataataaaa aatttgaaaa gttaaaagct gctggctact tagataagga
    12061 aattatttta ggtattcgag ctgaagacat tcatgaagaa ccaatattta ttcaaacctc
    12121 tccagagaca caatttgaat ctgaagtagt tgtatccgaa ctgttaggtt cagaaattat
    12181 ggtacatagc acattccaag gaatggaatt gatttctaaa ttagattcaa gaacacaagt
    12241 gatggcgaac gacaagatta cactagcatt tgatatgaat aagtgtcact tttttgatga
    12301 aaaaacagga aatcgtatcg tctaaggggg agtattcatg tctaaaattt taaaatatat
    12361 cacgttagcc gtggtaatgt tattaatcgt aactgcatgt ggccctaatc gttcgaaaga
    12421 agatattgat aaagcattga ataaagataa ttctaaagac aagcctaacc aacttacgat
    12481 gtgggtggat ggcgacaagc aaatggcgtt ttataaaaaa attacggatc aatatactaa
    12541 aaaaactggc ataaaagtaa agcttgtaaa tattggtcaa aatgatcaac tagaaaatat
    12601 ttctctagac gctcctgctg gaaaaggtcc agatatcttt ttcttagcac atgataatac
    12661 tggaagtgcc tatctacaag gcttagctgc tgaaatcaaa ttatcaaaag atgagttgaa
    12721 aggcttcaat aagcaagcac ttaaagcgat gaattatgac aataagcaat tagcattgcc
    12781 agctatcgtt gaaacaaccg cactttttta taataaaaaa ttagtgaaaa atgcaccgca
    12841 aacgttagaa gaagttgaag ctaatgctgc caaactaact gatagtaaaa agaaacaata
    12901 cggtatgcta tttgatgcta aaaatttcta ttttaattat ccgtttttat tcggcaatga
    12961 tgattatatt ttcaagaaaa atggcagtga atatgatatt catcagctag gactaaattc
    13021 aaaacatgtc gtcaagaatg ctgaacgatt acaaaaatgg tacgacaaag ggtatcttcc
    13081 taaggcagca acacatgatg tcatgattgg tctttttaaa gaaggaaaag taggacaatt
    13141 tgtcactgga ccatggaaca ttaatgaata tcaagaaacg tttggtaaag atttaggagt
    13201 aacaacattg cctacagatg gtggcaaacc tatgaaacca tttctcggtg tacgtggttg
    13261 gtatttatct gaatatagta aacataagta ttgggctaaa gatttaatgt tgtatatcac
    13321 tagtaaagat acattacaaa aatatacaga tgaaatgagc gaaattactg gacgtgttga
    13381 cgtaaaatca tctaatccaa atttaaaagt gtttgaaaag caagcacgtc atgctgaacc
    13441 gatgcctaat attcctgaaa tgcgacaagt ttgggaaccg atgggcaatg caagcatatt
    13501 tatctcaaat ggtaagaatc ctaaacaagc gttagatgag gcgacgaatg atataacgca
    13561 aaatattaag attcttcatc cgtcacaaaa tgataagaaa ggagattagt tatgacgaaa
    13621 cgtaacccta aattagcggc attattatct gttatacctg gtttgggaca gttttataat
    13681 aaaagaccca ttaaagggac gatatttttt atctttttca tcagttttat ttctgtattt
    13741 tatagctttt taaatattgg tttttgggga ttgttcacat tagggacagt acctaagtta
    13801 gacgattctc gtgtcttact tgcacaaggt attatttcta tcttactcgt tgcttttgca
    13861 atcatgctat atgtcattaa tattttagat gcatatcgta atgctgaacg atttaatcgc
    13921 aatgaggaaa taaaggatcc gaaggcacgt atggttgcaa catgggacaa gacgttccca
    13981 tacttactaa tctcaccagg tacattctta ttgatatttg tagttgtatt tccattaata
    14041 tttatgtttg gagtagcatt tacaaactac aatttataca acgcgcctcc gagacacaca
    14101 ttagaatggg ttggtttaga taactttaaa acgttattca caattggcgt ttggcgtaaa
    14161 acatttttca gtgttattac ttggacatta gtatggacgc ttgttgcaac gacacttcaa
    14221 attgcattag ggctgttttt ggcaattatt gtaaatcacc ctgtcgtcaa aggtaagaaa
    14281 tttatccgca ctgtgttaat cctaccttgg gctgtaccat catttgtgac aattttaata
    14341 tttgtagcgt tatttaatga tgaatttggt gcgataaata atgatatatt gcaaccttta
    14401 ttaggtgttg caccagcatg gttaagtgat ccattttggt caaaagtggc attaattggt
    14461 attcaagtat ggcttggttt cccattcgtc tttgcactgt tcactggtgt actgcaaagt
    14521 atttcatcag attggtacga agcagcagat atggatggtg cgtctagttg gcaaaagttc
    14581 agaaacatca cattcccgca tgtcatttac gccacagcgc cattgttaat tatgcaatat
    14641 gcaggtaatt tcaataattt taatcttatt tatctattta ataaaggtgg tccaccagtg
    14701 tcagggcaga atgctggtag tacagatatc ttgatatctt gggtgtataa tctgacattt
    14761 gagtttaaca actttaacat gggtgcagtt gtgtcattaa ttattggatt tattgttgct
    14821 attgtcgcat ttattcaatt cagacgtaca agtacgttta aagatgaggg aggtttataa
    14881 gatgacaaag aagaaaaaca tattaaaagc aatcggtatt tacagtttta tagcgatgat
    14941 gtttgtcatc attttatatc cactactgtg gacatttggc atttccctta atccaggtac
    15001 gaacttgtat ggtgccaaaa tgataccaga caatgcaaca tttaaaaatt atgcattctt
    15061 actattcgat gacagtagtc aatacctgac ttggtataaa aatacgctta tcgtagcatc
    15121 tgcaaatgca ctgtttagtg tgatatttgt cacgttaaca gcatatgctt tttctagata
    15181 tcgctttgtt ggtcgtaaat acgggctgat tacatttttg attttacaaa tgttccctgt
    15241 attaatggca atggtcgcaa tctatatttt gctaaataca attggattat tagattcttt
    15301 atttggacta acacttgtat atattggtgg gtctataccg atgaatgcct ttttagtgaa
    15361 aggttacttc gatacgattc caaaagaact tgatgaatct gccaaaattg atggtgctgg
    15421 ccatatgcgt attttcttac aaattatgct tccattagct aagccaattt tagcagttgt
    15481 tgctttgttc aattttatgg ggccatttat ggactttata ttacctaaaa tactattaag
    15541 aagtcctgaa aaattcacat tagcagttgg attgttcaac tttattaatg ataagtatgc
    15601 aaataatttc acagtgtttg cagcaggggc aattatgatt gcagtaccta tagcaatcgt
    15661 attcttgttc ttgcaacgct acttagtatc aggtttaaca acaggtgcga caaaaggtta
    15721 gtttgaaatt gggagcgggg cagaaatgat aaagaaccac taatgaattt attgtgtagt
    15781 ggctcttaca aattagccac agctaatatg tatctaaaaa tagcctagaa cgttgattga
    15841 tgtttaaggc tcgacatacg tactaatgac gataaagatt taaaggagga cgttatgatg
    15901 acgattaaag ttggaatcat tgggtgtggt ggtattgcga atggcaagca catgccaagt
    15961 ttacaaaaag ttgaaaatgt tgaaatgatc gcattttgtg acgtagacat ttcgaaagca
    16021 gcgtgtgcgg cagaagcata cggtactgac aatgcaaagg tttatgatga ttacaaagca
    16081 ttgttaaaag atgacacgat tgatgttatc catgtttgta cgccaaatga ctcgcattgt
    16141 gaaattactg tagcagggtt gcatgccggt aaacatgtga tgtgtgaaaa accaatggct
    16201 aaaacgacag cagaagctca aaaaatgata gatacagcta aatcaacagg taaaaaatta
    16261 acaataggtt atcaaaatcg cttcagacca gatagtcaat ttttatataa atcagcgcaa
    16321 cgtggcgact taggtgacat ttatttcgga aaggcacatg ctattcgtcg tcgagcagta
    16381 ccaacatggg gtgtatttct agacgaagaa gctcaaggtg gaggaccatt aatcgatatc
    16441 ggtacacacg ctttagattt aacgttatgg atgatggata attatgaacc agaatcagtg
    16501 atgggttcaa cattccataa attaaataaa cagcatgatg cggcaaacgc ttggggttca
    16561 tggaatccag atgaatttac agttgaagat tctgcgtttg gctttattaa aatgaagaac
    16621 ggagcgacga tcattttaga atccgcttgg gcgattaatt ctttagaagt ggatgaggca
    16681 aaatgttcat tatcaggaac gaaagcaggt gctgatatga aggatggtct acgtattcat
    16741 ggtgaagata tgggcacact ttataccaaa cacgttgaat tggaaaacaa aggcgtcgac
    16801 ttttatgaag gtaatgaagt ggatgaagct gaagaagaag caaaagcttg gattgatgca
    16861 gttgtaaatg atactgaacc agttgtgaaa ccggaacaag caatggtagt tacaaaaatt
    16921 cttgaagcga tttatcagtc tgcaaaatca ggcaaagcaa tttactttga ataacatcat
    16981 acggtaagga ggcacatcat gacaaaatta aaagttggtg tgataggtgt tggtggtatt
    17041 gcacaagacc gtcatattcc agcattgctg aaactcaaag acacagtctc attagttgca
    17101 gtacaagata ttaatacagt gcagatgatt gatgttgcga agcgctttaa tatacctcat
    17161 gcagttgaga cacctagcga gctgtttaaa cttgttgatg cggtggtcat ttgtacacct
    17221 aataaattcc atgctgatct ttctatagag gcattgaacc atggtgtcca tgtattgtgt
    17281 gaaaagccaa tggcgatgac gacggaagag tgtgaccgca tgattgaagc ggctaataaa
    17341 aattgcaaat tattaactgt cgcatatcat tatcgtcaca cagatgtggc aattactgct
    17401 aaaaaagcaa ttgaatcagg tgtggttgga aaacctttag tagcacgtgt acaagcgatg
    17461 cgtaggcgta aagtgcctgg gtggggtgtt tttaccaata aggcattgca aggtggcggt
    17521 agtttaatcg attatggttg ccacttgtta gacttatctt tgtggctact aggtaaagat
    17581 atggtgccgc atgaagtgct aggaaaaaca tataatcaat tgagcaaaca accgaatcaa
    17641 attaatgatt ggggaacatt tgatcacact aaatttgatg tcgatgatca tgttactagt
    17701 tatatgacat ttgccaatcg agcaagcatg cagtttgaat gttcgtggtc tgcaaatata
    17761 aaggaagata aggtacacgt tagtttatca ggcgaagatg gcggtatcaa tttatttcca
    17821 tttgaaatat atgagccccg ctttggaact atttttgaaa gcaaagctaa tgttgagcat
    17881 aacgaagaca ttgctggtga gagacaggcg cgtaactttg tcaatgcgtg tttagggata
    17941 gaagagattg tggtaaaacc ggaagaagca cgcaatgtaa atgcccttat agaagcgatt
    18001 tatcgtagcg atcttgataa caagagcata caactttaat gattattata tataatacaa
    18061 aattctcaat ataaaaagaa ggagtgcttt tcaatgaaaa taggtgtatt ttcagtatta
    18121 ttttacgata aaaatttcga agatatgtta gattatgtcg cagaatctgg attggatatg
    18181 attgaagttg gaacaggtgg taacccagga gataaatttt gtaagttaga tgagttgtta
    18241 gaaaatgaag acaagcgaca agcatttatg aagtcaatca cagacagagg cttacaaata
    18301 agtggtttca gttgtcataa caatccaatt tctccagatc cgacagaagc gaaagaagcg
    18361 gatgaaacgt tacgtaaaac aatccgttta gcaaatctat tagacgtgcc agttgttaat
    18421 acattttctg gcattgcagg atcagatgat accgctaaaa agcctaattg gcctgttaca
    18481 ccttggccaa cagcctactc tgaaatttat gattatcagt ggaatgaaaa gttgatacca
    18541 tattggcaag atttagctga gtttgcaaaa gagcaagatg taaaaattgc catagagttg
    18601 catgcaggat ttttagttca tacaccatat acgatgttga agttacgtga ggctacaaat
    18661 gaatacatcg gtgctaactt agatcctagt catttatggt ggcaaggtat cgacccaatt
    18721 gctgcgattc gcatattagg ccaagcaaat gcaattcatc acttccatgc taaagatacg
    18781 tatattaatc aagaaaatgt aaatatgtat ggtctaactg atatgcaacc atatggtaac
    18841 gttgcaacaa gagcatggac gttccgtaca gttggttatg gacatagtcc atatgtatgg
    18901 gcagatatca taagccaact tattattaat ggatatgatt atgtattaag tattgaacat
    18961 gaagatccta ttatgtcagt agaagaaggt ttccaaaaag cttgtcaaac tttgaaatct
    19021 gttaatattt acgacaagcc agcagacatg tggtgggcat aatacgaatt cgaggttagt
    19081 ctgaagtttg tctgaagtaa gactggtggc agtgttgaat aaatgcatat atcgccaagc
    19141 cattgccaaa aatttcacac cttaaatcaa gttcattgtt tgtaaagaag gtgtacttta
    19201 tataagtata tagcgatggt catacccatt cacaataaca atcctcacta ttgaaaagag
    19261 taaataagct tttcaatagt gaggtatatg ataataaaaa agcctgttgt cacaatggtc
    19321 atagacacga catactttaa aggtttctga atataatatt tcagaacgca ctttaaagat
    19381 ggacgtcgat ggcgactaat gtgatgacag gctttcattt ttaaatattc attaatttct
    19441 cttcttgttt aatacgtaca tataagaaat acgcatacgg tactaataaa atagttgtat
    19501 atgttgcgtg tgttaataat aatacaccga ttaattcagg aatgatgttt aagaagtaat
    19561 ttgggtgttt tgtaatttta tataatccag atttaataat aggatggtta ggtaaaatga
    19621 ataattttaa tgtccaaata ccacctaaag ttttaataac cataaataac atgatataag
    19681 caaagattaa tataactaag ccaataccat ttgcaaagct aaatgtatct ttattaataa
    19741 atgcctctac accagccaat acataaatta aaacgtgtgt tattgctaaa aacttcgaat
    19801 ttttaacgcc atattcaact gcaccgtctg cttttaattg ttttgagtga ttaatagata
    19861 tctttaagct gacaagtctg atacagaaaa agataagtaa tatagataga atcatgatgt
    19921 cctccgtcat tatgtcatat gtataagcgt tgattttgac aacataaagt attttataga
    19981 taaaacttgt caaatactat taactattta ttaattttag tacataaata tgtttctaag
    20041 tatgtgttta tgttcagtat tttggataat ttaataattt taaggatatt aagcgcttac
    20101 accgacgtga tatatttgac ttaacgaaaa tgattgaggt gacagagatg aacttttttg
    20161 atatccataa gattccgaac aaaggcattc cattatcggt acaacgtaaa ttatggctta
    20221 gaaacttcat gcaagctttc ttcgtagtgt tctttgttta tatggctatg tatttaattc
    20281 gaaacaactt taaggcggca caaccgtttt taaaagagga aattggatta tctacattag
    20341 aacttggtta tatcggatta gcatttagta tcacgtacgg tttagggaaa acattacttg
    20401 gatattttgt cgatggacgt aacacaaaac gtattatctc attcttactt atcttatctg
    20461 cgattacagt tttaattatg ggatttgttt taagttactt tggttcagta atgggattat
    20521 taattgtact ttggggactt aacggggtgt tccaatcagt tggtggacct gcaagttatt
    20581 caacgatttc aagatgggcg ccaagaacga aacgtggccg atacttaggg ttttggaata
    20641 catcacataa tatcggtggt gccattgcag gtggtgttgc actttggggt gctaatgtat
    20701 tcttccatgg aaatgttata gggatgttca ttttcccatc ggtgattgca ttacttattg
    20761 gtatcgcaac attatttatc ggaaaagatg atccagaaga attaggatgg aatcgtgctg
    20821 aagaaatttg ggaagagccg gttgataaag aaaatattga ttctcaaggt atgacaaaat
    20881 gggagatctt taaaaaatat atcctgggaa atcctgttat atggattcta tgtgtttcaa
    20941 acgtctttgt atacattgta cgaatcggta ttgataactg ggcaccgtta tatgtgtcag
    21001 agcatttaca ctttagtaaa ggcgatgcag ttaatacgat attctacttt gaaattggtg
    21061 ctttagttgc aagtttatta tggggctacg tatcagactt attaaaaggt cgtcgtgcaa
    21121 ttgtagctat tggctgtatg tttatgatta catttgttgt cttattctac acaaatgcta
    21181 caagtgtaat gatggttaac atttcattgt ttgcattagg tgcgttaatc tttggtccgc
    21241 aattattaat tggtgtatca ttgactggtt ttgttcctaa aaatgccatc agtgtagcaa
    21301 acggaatgac aggttcattc gcgtatctat tcggtgactc aatggcaaaa gttggtttgg
    21361 cggctattgc tgatccaaca cgtaacggtt taaacatctt tggatataca ttaagtggat
    21421 ggacagatgt tttcatcgtc ttctatgttg cattattcct aggcatgatt ctattaggaa
    21481 tcgttgcttt ctatgaagaa aagaaaatta gaagtttaaa aatctaatat caatcggatt
    21541 aaaagtatcg ccaatttatt gcagtatagt tggcaatcct gccccgacgg catgtgcgtg
    21601 aagagatgaa agatactgct tctacccttg caaatatatc atctctatgt ctcggggcag
    21661 atcataattc cctgttatga agtatcctta tttgcccgac ttagggtgac tcaatgaatt
    21721 tactccctac aataaagaca tatagcggtg tcaatattgt agggagtatt gttttatatt
    21781 tagactctct aaaaagcgga ttgaaagaaa agtgaaaact tctctatcag tccgcttttt
    21841 catagaacaa aatggatgcg ccataatcat tagttatttg ctaatctatt ttgcttgctt
    21901 acaataatca cttggcgaca tttgtaaata ttttttaaaa tgatagctaa acattttata
    21961 ctctgaaaag cctactttgt ctgcaatttc atagtgctta taatgtcgat ctaacaattg
    22021 tagagattgt aaaatacgat agcgatttaa ataatcgaca attgtaatac caacatgatc
    22081 tttaaatgtt cgcatcgcat acgattcact aacatcgata tgttgaatta aatctgaaac
    22141 agtcactttc gtttgataag attgcttaat ttgatcaaca atctgattta cataataatc
    22201 atcgtattct acttttaata atggttggaa ggcatcatga caaggtgcta agctacggcc
    22261 gttctgtgat tgttgctcta ataaggtacg gacaagtctt cctaaaataa cttctaattg
    22321 tgcatggtct actggtttta ataaataatc aagaacatga tgttgaatgc cggctttcat
    22381 atattcaaag tcatcgtaac tcgataatat gatgacatta caatctagat gcgcaatatc
    22441 attgagtaaa tcgacgccat ttttacgtgg catacgaata tcagtaatta ctaattctgg
    22501 ctgatgttgt tgaattagtg ataatgcttc aacaccatct ttagcagtgt atattgtatt
    22561 gaaatgatag tctccccaag gaatgatttg ctttaaacct tctcgaataa ttcgctcatc
    22621 atcacaaata actaccttaa acatctacat tcccccttga aagtggtatt ttataacaaa
    22681 ttaacgtacc ttgattacgc tttgaaaaaa tatggagtcg tgcatgtgaa ccatattgaa
    22741 tcattgcttt attgtgtaaa tgatttaatc ccaaatgctt agtatcaaat acatcattat
    22801 taagagattg gcgtacatat tgcaggcgag atgacgacat cccaatacca ttgtcgcaaa
    22861 ctaaaacatg taaattctga cgtgccaatg tcaggcgtat agtaatgtcc aatgactcag
    22921 tatctctacc atgtttaata gcattttcta tgagtggctg aagcatcatt ttaccaattg
    22981 tctggtgacg cgcttcttca gaactttcga tatggagctt aatcatgtca tcaaaacgga
    23041 tgttttgtat cgcaacatac tgttcaatgt agttcaactc ttcgtttaat tccactgtat
    23101 gtgagtttgt acgtaatgag taacgtaaca tttgcgataa ttgttggacc acagtttgtg
    23161 ctaatttcgg agataacgta attaaatatt gtattgtttg catcgtattg aataggaaat
    23221 gaggttggaa ttggcgttct atttccttta actgaatatc acgcaagcga cgttctgtat
    23281 gctcgataga atggatcagt tgctcatttg attcaaataa atcgtaaata taattattaa
    23341 tttcttctag ttcactgttg ttttttaaag gcgtatatgt acctagatga cgatttttgg
    23401 catagtaaat tttttgaata atcgtttcga tatcttttgt ttgtcgttta gccatattat
    23461 ctgcactaat gaaaccaaat attactagta aaacaagaac tacggccata acaattaaca
    23521 acgtgatacc atcttcaatg ttttcatgta tatctttata aataatgaga cgatggtcag
    23581 catggtttaa ttttacagat tcattcataa atccgaattg ttgcggtcta tacttttcac
    23641 ctatagtaaa acggtcatcg ttggcgtata aaatattgtc atattgatca acgataagtg
    23701 cgaattgtcg gctatctttc ttaatttcac ttaaacgtgg ggtgttagct atataaattt
    23761 taagcatata tgtactattt ttgaatttaa gctgatgcgt tgaaaataaa tacatatttt
    23821 tagtgtttaa atgttcataa ttattggtta taaactgatt tggtccagat aattcataat
    23881 aaagtgttgc gggctgttgg tgtattaatt ttaataattc acgttttgta gcggtaacat
    23941 catgatgatt tgttaaatcg agctcttgaa acgaattatt atgctgtgta ataaacgtct
    24001 gaatttgctt ttcagtatga tgtaaagatg actgactttc atcaacatgt tgatgaatcg
    24061 tacgatgctc aatccaaata tagatggcat agaagcttac tagtccaata ataatcacta
    24121 aaaatactgg aaaaatagta gacgcaaata acgatcgtct caattgatgt ctataaggtt
    24181 tgtatgccgt cattgaatca tctccaaaaa tttatgatgc ggaatgtccg gtaatttaga
    24241 tttcggtatt aaaggtatgt tcttaagatt ttcgatagac tgatcgcttt gttcactaac
    24301 atcttttcga attgacttgg catcgaactc tgcaactaat cgttgttgta ctgagcggct
    24361 tgttaaatat tgcactaact ttttacgctt aggatgaggg tgtgcatttt taactaaagc
    24421 aataccatca acatttaaca ttgttccttc aattggataa acgattgata caggataacc
    24481 tttgtttttc catgtgcgtg catcttgttc gtagcttaga cctgcataat atttaccttt
    24541 tgcaacatct tcaatgactt tagacgtctt tgacagttgc atcgcgtggt cttggaattg
    24601 atgcacatca cttactcgat gatgcatgct ataaatagcg cgcatatgtt gatagcctgt
    24661 cgttgttgta tttggatttg agtacgcaat tttaccttta agtataggtt gtaataaatc
    24721 ttgataacct tgaatcttaa tatctccttg taaatctgaa ttaactacta taactgttgg
    24781 cattaataga aagctagtaa catatttatt gttcgagcga taatcctcta attgctgtgt
    24841 tacagatgta tcttgatagg gaacaaaatc ttctggatga tcaattgttt ctgacaacac
    24901 accacccata aagacatcac cacgctccga aagatcttca ttatgcaagt ttgaaagcag
    24961 tacttgagta gatccgtgtt taatttcaat tttgacatgc tcttgttttt caaaatcatt
    25021 taaaattgga cgaatcaagt ttgattgata cggagaataa actgttaata catttttatc
    25081 tgattcagag tgacgcgtat tagtgcatgc tgataaaaaa atgagaaata atagcaagat
    25141 ataaattttt gatttcatga tatcccatca attctatgta tattttaata caataatttt
    25201 agcaataaat gacgcataag taatgttaaa tatttagaaa tgtttataga tgacttgtta
    25261 agacgttgca aatgttgtga tagcacataa tttttgtttg tcaagacgac ttaccgaagc
    25321 tgtaaagtca aactgttata ttttatttga agctgttata taaaaattgg caagatattg
    25381 aacggttcaa aagtaaattt ttacgtcaat aaaagtattt aatcctgtct cttcatatat
    25441 aaaagtaaat ctttctaagt attgatttaa cgcttattaa caatcatttt ttataaacaa
    25501 atatatactc ctaaattaac ttttaaagta atgaaaatag tgaacattat aactgttgtg
    25561 taacagaatg caattagcat attactgtta cacaaattag tacagtttct atgttttgac
    25621 atacatttga tgaaaattgt acataattta tgtgaaaaaa atcacaacaa acatgctaca
    25681 atgactatga aaacgttaac atagcatttc aaattcacaa cattatacag atggaggcgt
    25741 ttagtatgtt agaaacaaat aaaaatcatg caacagcttg gcaaggattt aaaaatggaa
    25801 gatggaacag acacgtagat gtaagagagt ttatccaatt aaactacact ctttatgaag
    25861 gtaatgattc atttttagca ggaccaacag aagcaacttc taaactttgg gaacaagtaa
    25921 tgcagttatc gaaagaagaa cgtgaacgtg gcggcatgtg ggatatggac acgaaagtag
    25981 cttcaacaat cacatctcat gatgctggtt atttagacaa agatttagaa acaattgtag
    26041 gtgtacaaac tgaaaagcca ttcaaacgtt caatgcaacc attcggtggt attcgtatgg
    26101 cgaaagcagc ttgtgaagct tacggttacg aattagacga agaaactgaa aaaatcttta
    26161 cagattatcg taaaacacat aaccaaggtg tattcgatgc atattctaga gaaatgttga
    26221 actgccgtaa agcaggtgta atcactggtt tacctgatgc atacgggcgt ggacgtatta
    26281 tcggtgacta tcgtcgtgta gctttatatg gtgtagattt cttaatggaa gaaaaaatgc
    26341 acgacttcaa cacgatgtct acagaaatgt cagaagatgt aattcgttta cgtgaagaat
    26401 tatcagaaca atatcgtgca ttaaaagaat taaaagaact tggacaaaaa tatggtttcg
    26461 atttaagccg tccagcagaa aacttcaaag aagcagttca atggttatac ttagcatacc
    26521 ttgctgcaat taaagaacaa aacggtgcag caatgagttt aggtcgtaca tcaacattct
    26581 tagatatcta tgctgaacgt gaccttaaag caggtgttat tactgaaagc gaagttcaag
    26641 aaattattga ccacttcatc atgaaattac gtattgttaa atttgctcgt acacctgatt
    26701 acaatgaatt attctctgga gacccaactt gggtaactga atctatcggt ggtgtaggta
    26761 ttgacggacg tccacttgtt acgaaaaact cattccgttt cttacactca ttagataact
    26821 taggtccagc accagaacca aacttaacag tattatggtc agtacgttta ccagacaact
    26881 tcaaaacata ctgtgcaaaa atgagtatta aaacaagttc tatccaatat gaaaatgatg
    26941 acattatgcg tgaaagctat ggcgatgact atggtatcgc atgttgtgta tcagcgatga
    27001 caattggtaa acaaatgcaa ttcttcggtg cacgtgcgaa cttagctaaa acattacttt
    27061 acgctatcaa tggtggtaaa gatgaaaaat ctggtgcaca agttggtcca aacttcgaag
    27121 gtattaacag cgaagtatta gaatatgacg aagtattcaa gaaatttgat caaatgatgg
    27181 attggctagc aggtgtttac attaactcat taaatgttat tcactacatg cacgataaat
    27241 acagctatga acgtattgaa atggcattac atgatacaga aattgtacgt acaatggcaa
    27301 caggtatcgc tggtttatca gtagcagctg actcattatc tgcaattaaa tatgcacaag
    27361 ttaaaccaat tcgtaacgaa gaaggtcttg tagtagactt tgaaatcgaa ggcgacttcc
    27421 ctaaatacgg taacaatgac gaccgtgtag atgatatcgc agttgattta gtagaacgct
    27481 ttatgactaa attacgtagt cataaaacat atcgtgattc agaacataca atgagtgtat
    27541 taacaattac ttcaaacgtt gtatacggta agaaaactgg taacacacca gacggacgta
    27601 aagctggcga accatttgca ccaggtgcaa acccaatgca tggccgtgac caaaaaggtg
    27661 cattatcttc attaagttct gtagctaaga ttccttacga ttgctgtaaa gatggtattt
    27721 caaatacatt cagtatcgta ccaaaatcat taggtaaaga accagaagat caaaaccgta
    27781 acttaactag tatgttagat ggttacgcaa tgcaatgtgg tcaccactta aatattaacg
    27841 tatttaaccg tgaaacatta atagatgcaa tggaacatcc agaagaatat ccacagttaa
    27901 caatccgtgt atctggttac gctgttaact tcattaaatt aacacgtgaa caacaattag
    27961 atgtaatttc tcgtacattc catgaaagta tgtaacaaaa tttaaggtgg gagcactatg
    28021 cttaagggac acttacattc tgtcgaaagt ttaggtactg tcgatggacc gggattaaga
    28081 tatatattat tcacacaagg atgcttactt agatgcttgt attgccacaa tccagatact
    28141 tggaaaatta gtgagccatc aagagaagtc acagttgatg aaatggtgaa tgaaatatta
    28201 ccatacaaac catactttga tgcatcgggt ggcggtgtaa cagtcagtgg tggcgaacca
    28261 ttgttacaaa tgccattctt agaaaaatta tttgcagaat taaaagaaaa tggtgtgcac
    28321 acttgcttag acacatcggc tggatgtgct aatgatacaa aagcatttca aaggcatttt
    28381 gaagaattac aaaaacatac agacttgata ttattagata taaaacatat tgataatgac
    28441 aaacatatta gattgacagg aaagcctaat acacacatcc ttaacttcgc gcgcaaactg
    28501 tcagatatga aacaacctgt atggattcgc catgtccttg tgcctggtta ttctgatgat
    28561 aaagacgatt taattaaact aggggaattt attaattctc ttgataacgt cgaaaagttt
    28621 gaaattctgc catatcatca gttaggtgtt cataagtgga aaacattggg cattgcatat
    28681 gaattagaag atgtcgaagc gcccgatgat gaagctgtta aagcagccta ccgttatgtt
    28741 aacttcaaag ggaaaattcc cgttgaatta taaatacaat tcagaccgaa aagaaagcat
    28801 atgcaacttc aagagtgaag gggcatatgc ttctttttca attgagtatt gattattaac
    28861 aagacgtagt aaatatatga gacaagtcca acaatggttg aaggaagacg catttgtaag
    28921 tagctatgct gataaagaat gtgatgcctt gttaaagatg gggttccaat atcatcgaat
    28981 gggttattat ttgctacttg catacgaata tgagtctttt caaattttta ttgaccctga
    29041 gtaatgaaaa atattaagat gaaacttaat attaaagcaa tgcggagcgt gattatgaag
    29101 agaattagta aagatatatg ggcagtattt aaattactgt atcaaaataa agggcgtttt
    29161 agcattaatg ccttactatt gcagttaatc atgattttta ttagtagtac atacttaatt
    29221 ttactattta atatgatgtt aaaagtagct gggcaaagcc aacttacgat taacaattgg
    29281 atggaaatcg taagtcaccc tgccagtgtg atacttctta ttatattcat attaagtgtt
    29341 gcctttctga tttatgtaga gttttcattg ttagtttata tggtttatgc cggctttgat
    29401 cgacagatta ttacatttaa atccattttt aaaaatgcct ttgtcaatgt gcgtaaactc
    29461 ataggtgtac cagttatttt ctttgtcatt tatttaatgt taatgatacc cattgccaac
    29521 ctaggactaa gttcagtatt aacaaaaaat atttacatac ctaaattttt aacggaagaa
    29581 cttatgaaaa cgacgaaagg tataatcatt tacggtacct ttatgattgc tgtatttata
    29641 ttaaatttta aattaatatt taccttaccg ttaacgattt taaaccgcca atcgttattt
    29701 aaaaatatga gactaagttg gcaaattacg aagcgaaata agtttcgact tgttatagaa
    29761 atagttatat tggaactcat cattggtgcg attttaacat taattatttc aggagcaaca
    29821 tatcttgcta tttgtgtaga tgaagaagga gataagtttt tagtctcatc aattttattt
    29881 gttgtattga aaagctcatt gttcttctat tatttattta cgaaattatc attaatcagt
    29941 gtgttagtac tgcacttaaa acaagagaat gtattagacc agccgggctt agaatttaaa
    30001 tacccaaaac cgaaacggaa gtctaggttc tttataattt caatggtgct tgcagtgacg
    30061 tgttttatcg gttataacat gtacttactt tacaataata ctatcaatac aaatatctcc
    30121 attattggcc atcgtggttt cgaagataaa ggcgttgaaa attctattcc gtcattgaaa
    30181 gctgctgcaa aagcgaatgt cgaatacgtt gagttagata caattatgac gaaagataaa
    30241 caatttgttg ttagtcatga taacaatttg aaacgtttaa caggtgttaa taaaaatatt
    30301 tctgaatcta atttcaaaga tgtcgtcggt ttgaaaatgc gtcaaaatgg acatgaagca
    30361 aaacttgtat ccttagacga atttattgaa acggctaaac aatcaaatgt gaaactacta
    30421 gttgagttaa agccacatgg taaagaacca gcagattata cacaacgtgt tattgatatt
    30481 ttgaaaaagc atggtgttga acatcaatat cgtgtgatgt cattggatta tgatgtgatg
    30541 actaagttga aaaaagaagc gccatatctc aagtgtggtt atatcattcc gttgcagttt
    30601 ggtcatttta aagaaacatc tttagatttc tttgtcatcg aagatttttc ttattcgcca
    30661 agacttgtta atcaagcaca ctcggaaaat aaagaagtct atacttggac cattaacggc
    30721 gaagaagatt taacgaaata cttacaaacc aatgttgatg gtattatcac agatgaccca
    30781 gcattagctg atcagattaa agaagaaaag aaagacgaaa catacttcga tcgttctata
    30841 agaatattat ttgagtaata taaacaaaga cctctaaagt tatcaagatg atatcttcag
    30901 aggtcttttt aacgttgcca tctatggtat aggcaatcgt ttcattcgtt tataatcata
    30961 tgacaaggat ttataaggaa atttggcgtc acaaacactt acatgattta ttggtgaatt
    31021 attaattgtt ttgtgaatgc aaagggttag aaattgaatc gtaaatactt tctaatctat
    31081 gtttcgcttt agtcatttga tccaaatttt tagtgcgtat agctgattta gcaatatagt
    31141 gtgcagctaa aatatcgcgt ttttgatacg catctaaatt taggtgcgat aatttattta
    31201 agtcagtgtt tgctattaat tcatgtaatt gatctacaag cgcttgatgt tgatacgtat
    31261 gtgatgtagt ttcagatttg cttgctaatt taataccagt cgtatcaagg agcgccgctt
    31321 taataccagc aactaaatat gttttgattt tcatttgtgt tgtcatgctt tgttactcct
    31381 ttgatgtaca ttaatcaaaa aaattataca ctattgtata ttgcaaagct aattaactat
    31441 aacaaaaaga tagttaatgc tttgtttatt ctagttaata tatagttaat gtcttttaat
    31501 attttgtttc tttaatgtag attgggcaat tacattttgg aggaattaaa aaattatgaa
    31561 aaagcaaata atttcgctag gcgcattagc agttgcatct agcttattta catgggataa
    31621 caaagcagat gcgatagtaa ctaaagatta tagtaaagaa tcaagagtga atgagaacag
    31681 taaatacgat acaccaattc cagattggta tctaggtagt attttaaaca gattagggga
    31741 tcaaatatac tacgctaagg aattaactaa taaatacgaa tatggtgaga aagagtataa
    31801 gcaagcgata gataaattga tgactagagt tttgggagaa gatcattatc tattagaaaa
    31861 aaagaaagca caatatgaag catacaaaaa atggtttgaa aaacataaaa gtgaaaatcc
    31921 acattctagt ttaaaaaaga ttaaatttga cgattttgat ttatatagat taacgaagaa
    31981 agaatacaat gagttacatc aatcattaaa agaagctgtt gatgagttta atagtgaagt
    32041 gaaaaatatt caatctaaac aaaaggattt attaccttat gatgaagcaa ctgaaaatcg
    32101 agtaacaaat ggaatatatg attttgtttg cgagattgac acattatacg cagcatattt
    32161 taatcatagc caatatggtc ataatgctaa agaattaaga gcaaagctag atataattct
    32221 tggtgatgct aaagatcctg ttagaattac gaatgaaaga ataagaaaag aaatgatgga
    32281 tgatttaaat tctattattg atgatttctt tatggataca aacatgaata gaccattaaa
    32341 cataactaaa tttaatccga atattcatga ctatactaat aagcctgaaa atagagataa
    32401 cttcgataaa ttagtcaaag aaacaagaga agcaatcgca aacgctgacg aatcttggaa
    32461 aacaagaacc gtcaaaaatt acggtgaatc tgaaacaaaa tctcctgttg taaaagaaga
    32521 gaagaaagtt gaagaacctc aattacctaa agttggaaac cagcaagagg ataaaattac
    32581 agttggtaca actgaagaag caccattacc aattgcgcaa ccactagtta aaattccaca
    32641 gggcacaatt caaggtgaaa ttgtaaaagg tccggaatat ctaacgatgg aaaataaaac
    32701 gttacaaggt gaaatcgttc aaggtccaga tttcccaaca atggaacaaa acagaccatc
    32761 tttaagcgat aattatactc aaccgacgac accgaaccct attttaaaag gtattgaagg
    32821 aaactcaact aaacttgaaa taaaaccaca aggtactgaa tcaacgttaa aaggtactca
    32881 aggagaatca agtgatattg aagttaaacc tcaagcaact gaaacaacag aagcatcaca
    32941 ttatccagcg agacctcaat ttaacaaaac acctaagtat gtgaaatata gagatgctgg
    33001 tacaggtatc cgtgaataca acgatggaac atttggatat gaagcgagac caagattcaa
    33061 caagccaagc gaaacaaatg catacaacgt aacgacaaat caagatggca cagtatcata
    33121 tggcgctcgc ccgacacaaa acaagccaag cgaaacaaac gcatataacg taacaacaca
    33181 tgcaaacggc caagtatcat acggagctcg tccgacacaa aacaagccaa gcgaaacgaa
    33241 cgcatataac gtaacaacac atgcaaacgg tcaagtgtca tacggagctc gcccaacaca
    33301 aaacaagcca agtaaaacaa atgcatacaa tgtaacaaca catgcagatg gtactgcgac
    33361 atatggtcct agagtaacaa aataagttta taactctatc catagacata cagtcaatac
    33421 aaaacattat gtatctttac aacagtaatc atgcattcta tgatgcttct aactgaatta
    33481 aagcatcgaa caatcggaag catatttcta aattatttat tcattatagt cttaaacata
    33541 acatgaccta atatattact aacctattaa aataaaccac gcacatctat gtgacatacg
    33601 acaatcacag caataataat tgctttagaa agtcgtaccg aactggaact tacaagtcta
    33661 gttcgaacac acactgatgt gagtggtttt ctttatttta aacatgaaca atcagataag
    33721 ttactagtag tagcaaatat tattaaatca aagggcttcg attcataaaa tttaaaacaa
    33781 tgattaaaat tagacgtgta aatgttaaat tctaaaacgg aaatacccgc tatcctatta
    33841 aaccactttt tggttcaatc actatatttc acacagtttc aataataaaa cgaatctgct
    33901 tcaatccgct tcaacttcag cctacttcat tcaataacaa aacgaatccg cttcattcaa
    33961 aatcaaccat tctaacgcac atactcaaat atagcagctg cacccatacc gacaccaata
    34021 cacatcgtaa ccatgccgta acgactatcg ggacgtctac ccatttcatt aagtaaacgc
    34081 gcggttaaca ttgcgcctgt agcacctaat ggatgaccta aagcaatagc gccaccattc
    34141 acattcgtac gtgatatatc tagacctact tctttaatag atgcaatcgt ttgagaagca
    34201 aatgcttcgt tcaattcgat taaatcaatg tcttcaacag atagattgct gagtgacaat
    34261 acttcaggaa tcgcatatgc aggcccaata cccataattt tcgggtcaac gcctactgcc
    34321 ttataaccaa cgaatcgtgc aataggtgtc acgcctagtt ctttcacttt atctccagac
    34381 attaaaacta caaatcctgc accatcagaa agtggggcag atgttcccgc agtcacagtg
    34441 ccgtcagctt taaatactgt acgtaatttg gctaatgcct ccatcgtggt gtcaggtcgt
    34501 ataaattcat cttggtcaaa gatatttgtg tgtacttttg gtcctacgtt tgtatattca
    34561 actgagttta cttgtattgg aataatttca tctttgaacc gaccatcacg ttgtgcgtca
    34621 taggcacgct gatgacttct gacagcataa gcatcttgat cttcgcgtga tacgtcaaat
    34681 tgggatgcta cattttcagc agttaaaccc ataggatatg acgcacctat atcatcatat
    34741 tgtaaggtag gattgtttgt gggctcgttg ccacccattg gtacggcact catcaattca
    34801 acgccaccag ctacaagtat atctccttga cccgccataa tttgattggc tgcaatcgcg
    34861 atggtttgta atcctgatga gcagtagcga ttcactgttt gacccggtac agtgtcagat
    34921 aatcccgtac gcaatgcaat cgttcgtgca atgttttgtc cttgtaatcc ttctggaaaa
    34981 gccgtaccaa caatgacatc ttcaatcata ttcttattga attttccgtc aatacgtttc
    35041 aatacgcctt gtaatacttt ggctgcgaca tcatcaggtc tttcgtggaa taatgcgcct
    35101 tgctttgctt tcgctgtggc tgaacgccca taagctacaa tgtatgcttc ttgcatggtt
    35161 atcatcctct cttaatgact atcttttaat tacgtaatgg cttaccagtt tttaacatat
    35221 gcgcaattct ttcatatgat tttttagatt ttagtaagtc aataaagcca attttctcca
    35281 acgattgaat gtaacgttga ttgataaatg tatttcttgg taaatcacca cccgataaaa
    35341 ttgtggcgat atttaaggcg atatgataat catggtcgct aataaaatga ccgtgtcttt
    35401 gcgcatctaa ttgtccttgg atcaatgctt tgaagtcttc gcctaaagcg atatattgat
    35461 gtctaggatt cggaatatag tttgtttctg cttcatattt cgcacgtttg agcgcaactt
    35521 cgacacgttg tgctgtattg aaaataatcg tatctgtatc acttaaataa ccataacgac
    35581 gtgcctcaaa ggcatttgta gagactttcg caaatgcgat attcgtcagt acttttgtca
    35641 tggaagcttg tttgtcatca aacttatgcg atgtgcgtaa tatgcgatca gccatttctg
    35701 caaggccacc tccactcggt aataagccaa cacctgcttc aacaagaccg atataagttt
    35761 cacttgcagc gacaacaata ggtgagtaaa gtacaagctc acaaccaccg cctaaggcac
    35821 gaccttgaac agctgtgact actggtttca aactatactt caaacgatta aagctataat
    35881 gtaatttatc aattgattgt gcaacgacat catctacaag accgtcttca tgcgcctttt
    35941 tcattaagaa aaggttagcg cccacactga aattattacc atctgcataa ataaccatac
    36001 ttgtgtaatg ttcattttcc agtaaatcaa tcgcatcaac taacgcatcg ttgaattcat
    36061 cggtaatgac attattttta ctttgtaatt tcagtaacag ttgatcatta tgagttacgg
    36121 aaagtttggc atcaccttta tcccaaagtt catcttttac gaagtgagaa acaggcgttg
    36181 catattcaat ggtctcatct tgtttataaa agccaccgtc taaatcacta atccattgtg
    36241 gtaagtctcc aagttcgtct tccatacgtg ttttaacacg ttcatatccc attgcatccc
    36301 ataattggaa tggaccaagt ttccagttga acccccagac aagcgcacgg tctatgtctc
    36361 ggaaatcatc ggtagcttta ggtacattga tagcagagta atagaaatta ttacgtaatg
    36421 tctcccataa aaatagtccc gcttcgtctt gcgcattgaa tatgacatca aggttatgca
    36481 ctaagtcttt attaaattca tttaaaattg gtaattgtgg ttgcgataca ggtacataat
    36541 cttgtttttc aacatcgtaa acaagtcgag ctttagtttc tttatccttt ttgtaaaatc
    36601 cttgttttgt tttacgtccg agtgcgccat tttcatacaa cgtatttaca attttgacat
    36661 catgaaaata aggtgtttct tcaggtactt gttgcatgcc tttaattaca gacactgcaa
    36721 tatctaaacc gactaggtca gatagggcat atgtacctgt tttaggacga ccaatcgctt
    36781 gcccagttaa agcatccaca tctacaatgc ttaacttgtg ttgctcggcg cgatacataa
    36841 tatcattcat tgtttgcgtg ccgactctat ttgcgacaaa gccaggcaca tcattgacaa
    36901 caatgacacc tttacctaac acattttgcg cgaaattttt tacatctaat ataatagatt
    36961 ccttcgtgtg tgacgtaggt attaactcca ctaatttcat aatacgtggt gggttaaaga
    37021 aatgtagacc aaagaatcgt tcttgatcct tctcgttaaa tgcttgagca atcgcattaa
    37081 ttggaatacc tgatgtattt gtagcgaata aagcatcttc tttagcatgt tgtagaacta
    37141 gttgccaaac agcatgctta atttcaatat cttctttgac tgcttcgata tataaatcag
    37201 catcatcatt taccaagtca tcatcaaaat taccatatgt taaatgactc gctagattta
    37261 agtcgaatag tagcggtcgt ttcttatctg taattttatc gtaagatttt ttcgcaatga
    37321 gatttggatc gtttttgtcc actacaatat ctaatagttt tactttaagt ccagcattca
    37381 caaagagtgc tgccagttga gcgcccattg tgcctgcgcc aagaacggtt actttattaa
    37441 ttgtcatagt gattcctcca atttagttga ggataagata accattaaga taattggaat
    37501 aacgttgcta ttttataaaa ttaattaagt atctttgaca gtcatcttag cctcttattt
    37561 aaggaaaaag ctttatgctt aaaataagtc tttttttagt gaaattactg catcacatat
    37621 aattctttgc tatttatacg aaagcagaat ctccagtcaa agcgcgtcca attactaagg
    37681 cattaatttc atgtgtgcct tcgtatgtgt aaatcgcttc tgcatcagag aagaaacgtg
    37741 caatatcata atcgtcagct agtatgccat taccacctgt aataccacgg cccatagcta
    37801 ctgtttcacg caaacgtaag gcattcatca tcttcgcggt tgaagttgca acctcatcat
    37861 attcaccatg tgcttgcata ttagctaatt gagcacatgt tgccattgct tgagctaaat
    37921 taccttgcat cattgctagc ttttcttgta ttaactgata tttactaatt ggtttgccga
    37981 attgcttacg ctcagtgaca taatctaatg tggcacgtaa agcgccagcc ataccacctg
    38041 tagccatata agcaacgcct gctcttgttg aataaagaat tttggcaata tctttaaagc
    38101 ttgttatgtt ttgtaagcga tccgcttcat ctactttgac attagttaat ttaattaggg
    38161 cgttaggaac aatgcgaagt gcgattttat tatcaatgac ttcaatatcg acgccatctt
    38221 gttctggtct gactacaaag caatggggtt tgccagtttc tttatttact gcgaatactg
    38281 gaatgacatc agatacatgt gcaccaccaa tccatttctt ttcaccattg ataacccaag
    38341 tatcgccctg gcgttcagcg actgtttcaa gacctcccgc aacgtccgaa ccgtgttctg
    38401 gttcagttaa agcaaagcat gtacgcagtt catgtgactg taatttaggt acatatttcg
    38461 caatttgttc tttgctacct ccgaaataga aagtgttatg ccctaaacct tggtgaacac
    38521 cgagtagggt agctaaggaa atatcaaatc gcgcgagtag gtaagacatg aaaaactgaa
    38581 atagttgact aggcattttg gcgtttggac gatccttgta tagtaatgga ttgttaaaat
    38641 aatttaattc tcccagatct ttaaaatagt cctcgggtac agtagcgtct atccaatgtt
    38701 gattaatatt ttcacggtac ttactttcta gcaatgaatc tacttgttgt aaaaattcga
    38761 cttcaccgtc tgttaaacct ttagcaatac taagtacatc ttcaggaaat aatgttttta
    38821 agactgtttc tttttcaaat gtcatataaa ttcctcctaa aaataatatg aatactaatg
    38881 tgaaatgcat ttaattcaaa acaacacgct ttatttgtaa acgcttacac taaatgctaa
    38941 aaatttttta tcacctttaa agtgtttgtg agactttgtc attcatcatt tgtcgaatcg
    39001 caagtttatc tggtttctgc gtactgttta acggcatatg tgtcactggt acatacattc
    39061 ttggaacttt ataacctgct aaacgacttc gcatatgttg atctaaaatt tcagcgtaat
    39121 gaggttcatc ttcacgaagt ataatggctg cagcaattga ctcaccatat tttggatgat
    39181 catagccaac gaccacacac cggtctacta gtggatgctc cgctaaagca ttttcgactt
    39241 cggatggtaa gacattttcg ccaccagtta tgattaattc ttttttgcgg tcaataataa
    39301 atatatcgcc atcgtcgtcc atcttcgcta aatctcctgt caataagtaa cgaccatgaa
    39361 acgctttagc agtctctgct ggtttattcc aatatcctgg cgtgacattt ttagccttaa
    39421 ttgcaagttc gccaatctca ccagtaggta cttcttcacc gttatcatca aggatacgtg
    39481 catcaacaaa catgactgct ttaccaatac tcattggttt acgttttgaa ttttccggtg
    39541 tattaacaag tacaagcggt gcttcagtta agccatagcc attaataatg tttatgccat
    39601 attgcttaaa agctgcttgg atacttggta atggttgtga accaccttgg atgatataat
    39661 ccatagcttt aaaattttca ggattaaaat tactagcacg tagcgtacta taatacattg
    39721 tcggaatcat gataataaat gtagggtgat attgtgcaat catgtcattc aattcttcgc
    39781 cgttaaagta acgttgaaga ataagtgttc cacctgacat taatactggt aatacagtat
    39841 cgttaaaccc taaaacatgg aacattggtg ttgatacaat cgtaatatag tttgaattga
    39901 acttgtacgt tagctctaag tttgcgccgt tatgaacaaa tgattcatat gagaacatca
    39961 cacctttagg tgatccggtt gtaccacttg tataaattaa tgctgcaaga tcttgtggtt
    40021 caacaggtgt tgcttgaaaa ggttggtgat aatctggatt tacgatttca tcatattgcg
    40081 ctacatcaat atccatatgc aataagtttt ggtcaatatc ggtgagtgaa cttaaatgtt
    40141 tttcagcata gaagagcagt tttaattgtg catcttccac aatggctgca atttcttttg
    40201 ggttaagccg ccaattcaat ggtaaaaaaa ccgcacctgt tttaaaacaa gcaaataata
    40261 aatctaatat tgcaatatca tttggcgcaa aaataccgat aacatcgcct tttttaacac
    40321 cttgagatgt taaataatgt gccatattat cagcgcgtgc attgagttgt tggtatgtcc
    40381 aagatgtttg ttttgcgtga tcaataacgg caggcttgtc atcatcgaag tctgagcgcg
    40441 tttttatcca atcgaaattc attagtatac cccctttagc ttcactttca tactttatga
    40501 attgattgtt taagttctcc ccatttttct ttgtaaatgc tggtatcaat taattttaaa
    40561 tgatcagcaa taattggttt aaaagccatt tgattcaaaa tatctttatg caaatcaaga
    40621 cctggtgcaa tttcaattag tttcaagcct tgattggtga gctcgaatac tgcacgatca
    40681 gtaacaaaat agacttcttg ctcgagtgat tgtgaatatt gtgcattaaa gtcgatatgg
    40741 ctcacatctg atacaaattt ctggttttgt ccttcagttt caatgtttaa tcgttgatta
    40801 tgacatgaga catgactgcc agctacaaaa gtacctgaaa agataatttt atttacagat
    40861 tgtgtaatgt ctataaagcc gccacatcca tttagtcggt cattgaagta agacacgttg
    40921 acattgccgt attgatcaac ctcagcaaag ctaagatagg caactgatac accattgtta
    40981 taaataaaat cccatgctcg atcatgaggc atgcgcacat ctgcattgta attcatacca
    41041 aaatgttcac gactcccaac gaatccaccg aaaatgccaa catctagaat cggttgcaca
    41101 tcatgttcaa cacattcttc atgcaataaa ttagagagtt cattattgat gccataaccg
    41161 atgctaattg tatcgccata agttaaaaac tgagcagcac gtcggagaat caacttgcga
    41221 ctattaaaag gtaatgcggg ttcaggtatt ccatcaatcc gttcttctcc agacaaggct
    41281 ggtaaataat gactctgaat tacttggcgg tgattctttt catcttctgt gacgtataca
    41341 taatcgacaa gatttcctgg gataacaact tcattcggct ttagttgata gtcgtcaact
    41401 aaatctttaa cttgtacaat aactttccca tgattggctt tcgcgtttaa tgcgacatga
    41461 taacactcgc tcaagtaaga ttcttgagtt aaataaatgt taccttgttg atctgcgtat
    41521 gttcctctca gtagtgccac atcaacgcta gggaatgtgt agtgtaagta tgtttcatcg
    41581 tcgatagtta ctaatgaaac taaatcatca gttgttcgtg tatttacttt accgccaccg
    41641 tatctaggat caacagctgt gtttaatccg attttagtaa taactcccgg taataattga
    41701 ttactctgac gataatgagt tgcaatgata ccttgtggca aaaaataagc ttcaatgtca
    41761 ttatttttca ttgcttgtgc cgttttggaa gaagctgtta aaatgctcat aatgacacgt
    41821 ttaatcatgc gacgttctat aaaatcatct aaatccggtg cgacacctaa actatggata
    41881 tcattcgcta atataaatgt taaatcattg ggcgtatgat atgtgtcatg ttgcgctaac
    41941 acagcacgta gaacttcggc gggtaagttg gctacagcta atgctggtaa accaatcaca
    42001 tcatcatctt taatgatatg ttgtaagtcg tgccatgtga tttgtttcaa gcaagtcacc
    42061 tccatcacat ttgataaaat atagcgtttt tacactttgt gtaaaccctt acaagaaata
    42121 taacataacg acgtttaaaa tcaattagaa atatcttttt attctgataa tagacacagt
    42181 atagacacat tttaatagtc gataacaatt gtaatatcaa gggtttgtaa tgaattgaat
    42241 atcattaaaa tacttatata aaaatattgc tcggaatata aaaagtaacc catgtttatt
    42301 taaaaaatgc aaagactaat tatcattttg atgcatcagt aaggcgaggg tgatcagcgt
    42361 acaattagta gtgtataatg taactgataa aatcattgga gagttgaagg agatgttaaa
    42421 catatgagag tcaatgtttt acaacataca ccaaatgaag gtgttggata cataggtgag
    42481 tgggcgaagg aaagagggca cgaagtctat atttatcatc catatatgaa tgatggcgtg
    42541 ctacctcttg cggaagaaac ggatatgtta gtgatacttg gtgggccaat gaatccagac
    42601 gatgagatgc cttggattca atcagagcgc caattaattt tagtattgat ggcacaaaac
    42661 aaaccaattt atggtgcttg ctttggtgca caacaaattt cgaaagcatt aggttatgcg
    42721 gtgttaaaat caccagtaaa ggaagtaggt tgggatgatg tttacttaga atcagaaatc
    42781 attccgaata taccaagtcg attgaaggca ctacattggc atgaagatat gtttcaaata
    42841 ccgcaacaag cagaattatt attttcgggg aaccatatta gaaaccaagg atttatcatc
    42901 gatcatcatg tgattggatt acaatttcat tttgagcctg gtccagacga tgtcagagaa
    42961 atagtactaa atgatgccca atacatcaat cattctattt tagagcaatc ggcggaggac
    43021 atattagcct ttgaggtgcc taaacagaat aggcaggcca ttttccgaat acttgatttt
    43081 attactcaag cgtaagatgt gagttgtatt ttaaaatgag taaaaaaaac gaatgtacac
    43141 aatcgaacaa agtagttata taaatatatg gaaaatccat caatattaaa ttaaaatagt
    43201 tttattatga aaagtgaaag taggttagtc tatggaaggt cttaatcatc aaagaaatac
    43261 agaaaaagaa gagacaacac aaacgcaatc agttgcacct aatacaggtg aagaagggat
    43321 gtcatcagca agtacacaat ctactaagac atccgacata cataatgaat ctatcgataa
    43381 acaaatggaa gccaaagcgc atgaaacagc gcaaaataca gatttaaaaa atgaagcaag
    43441 aagtttattt gataatgcaa ccaaatcaat cggtagacta gctggcaatg atgaaagctt
    43501 aaatcttaat ttaaaagata tgttttctga agtatttaag ccacatacta aaaacgaagc
    43561 agatgaaata tttattgcgg gtactgctaa aactacgcca gcaatttgtg acatatcaga
    43621 agaatggggg aagccatggc tcttttctcg agtattcatc gctttcacag taacatttat
    43681 tggattatgg gtcatggcag caatttttaa taacactaac gcgattccgg gtctcatttt
    43741 tataggggct ttaacagtac cattatcggg tttgttcttt ttttatgaat caaatgcgtt
    43801 taaaaatatt agcatttttg aagttattat catgttcttt attggcggcg tattttcatt
    43861 actaagtacg atggtattat atagatttgt cgtttttagt gatcaattcg aaaggtttgg
    43921 ttctttaaca tttttcgatg catttttagt aggattagtt gaagaaactg gaaaagcgct
    43981 cattattgtt tatttcgtca ataaattgaa aacaaataag attttgaatg gattattaat
    44041 cggtgctgct attggtgcag ggttcgcagt ttttgaatca gcaggttata ttttgaattt
    44101 cgctttaggg gaaaatgtcc cattattaga tattgtcttc acacgtgcgt ggactgcgat
    44161 tggtggtcat ttagtttggt cagcgattgt tggtgctgca atagttattg cgaaagaaca
    44221 gcatggcttt gaattcaaag atatatttga taaacgcttt ttaatattct ttttatcagc
    44281 cgttggttta catggcattt gggatacatc tttaactata cttggcagtg atacgttgaa
    44341 aatatttatt ttaatcgtta ttgtgtggat acttgtattc attttaatgg gggcaggttt
    44401 aaaacaagtg aatttactgc agaaagaatt taaagaacaa cagaaaaaag tagacgaata
    44461 ataattaaag cttatgttgc acatatgttt gtgacataag ctatttttat aatttgtctt
    44521 taaaagagtg gaagtggaat actttttgga gttaaaaaag tgtttcacgt taaacaaata
    44581 gtgacaatta ggtttatata aaatgaacat gattcgctga aagtatgtaa taatcatttt
    44641 attgaaattc atcaaacaga aagtaataca atcatataag caaattaaac cacgccatca
    44701 tcatctagaa tgacttcggc gtggttttta ttgaagcggg gctgagacat aaatcaatgt
    44761 cccacattcc cttatcgttc aatcgtagtt cgataatcga ttaaatagat accttcaggt
    44821 gttactttat aatttttaac cttagagtta gcagcaacta tttgatcgtt gtaagcaata
    44881 taactgttag gtacatctcg acttgataat ttaataatat cattagaaat attgtgacgt
    44941 tccttaacat ctacagtatg attcaattga ttaattaaat catcaacgtt gttattattg
    45001 tagtctcctt tattaatagc accatctttt ttatatgctt gattaaagaa atagcctgta
    45061 tctccacgag gaatcgttcc gaaactatac attgttgcat cccatgcaga acggtctttt
    45121 aagtaatctt ctatgtcatc aacactttta atgtcgattt caatatttgc ttttttagca
    45181 tctgattgta atacttgcgc aattttcgat agctctggac gaccgtcata cgtaattaac
    45241 ttaattttta aagggtgttc ttttgtgtaa ccatctttag ctaataacgt ttttgcttgt
    45301 tcgatatttt gtttggttaa cttaggttct ttaatatatg gaattttatc attaaataga
    45361 ctcgttgcag gtttcgcgta accttgataa atatgatctg caataccttg tctatcaatg
    45421 atatgatcta atgcttcacg aacggattta gtcatttttt tattagtatg attatacata
    45481 agtaaagaag ttctaaatcc agattctttt gacactttta aattttgatt attttctatg
    45541 tcttgaactt tattaactgg gacatcagtt attaaatcat ctttttgaga ttctaaattt
    45601 ctgacgcgat tattgccgtc ttcttggtac gtcacagtaa tatgatcaag tttcggttta
    45661 ccttgccaat agtccttaaa attcgacaat gatatttttc gagattgctt ataatcttta
    45721 atttggtaag ggcctgtacc aacaggagtt tgattaacat ctgatttagc atctgtatca
    45781 taaattgcca taaaaggatt agctaattca gatacaagtt cagggtaagc ggaactggtt
    45841 ttaattgtta gtttttgacc tttagcggta attgatgata ttggtaatga atatttgacc
    45901 aagtcgcttt ttttcatgct attttcaagg ctagatttca ctttttctgc agtcaatttt
    45961 tgaccgtttt gaaatttaat attatctttt aattctatat ctaacgttgt atcatttggt
    46021 tgatgatacg atttcactaa tactttttct atttttcctt gatcatttgt tttaaataat
    46081 gattctgcag caccgatctt aactggtaca tctgtttcat aaggtgcaat agactttgtt
    46141 tttaacggta acgaaatatt taagtctttg ccagatgaat gcattgagcc acatcctgat
    46201 aacactaata ctgctgaaaa tatagttgct agtcttttaa acttcatttc attaacactc
    46261 tctttctaat tactatgtaa aacccaacaa ttaatatttt aaaactttat ttt
//
LOCUS       DATTQF010000008        40633 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.008, whole genome shotgun sequence.
ACCESSION   DATTQF010000008 DATTQF010000000
VERSION     DATTQF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.008"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..384)
                     /locus_tag="VB241_000328"
     CDS             complement(<1..384)
                     /locus_tag="VB241_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP1243661.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILK"
     gene            complement(462..644)
                     /locus_tag="VB241_000329"
     CDS             complement(462..644)
                     /locus_tag="VB241_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243662.1"
                     /translation="MKIKNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(715..900)
                     /locus_tag="VB241_000330"
     CDS             complement(715..900)
                     /locus_tag="VB241_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243663.1"
                     /translation="MSTYKEIEHLHINTGGKELTQEQIEEAKAFIDSQEFKDMIREAK
                     ESHQRVMESKITDRTKL"
     gene            complement(897..1043)
                     /locus_tag="VB241_000331"
     CDS             complement(897..1043)
                     /locus_tag="VB241_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243664.1"
                     /translation="MDFKEVDINIEEWEMVEIPFYTEEELTYRLNNGLPITKSELEEQ
                     ESKK"
     gene            complement(1117..1971)
                     /locus_tag="VB241_000332"
     CDS             complement(1117..1971)
                     /locus_tag="VB241_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEP1243665.1"
                     /translation="MKPRKQDEKILSDQYSYFEPIISDSCDIKFDENKRRMGSIFISH
                     EEICFIRKEEDYIFKISLSEVIDYNTVVTIWKNQAFLTLNDNRKLTVYFVTNSPLTGF
                     ISILKTYMQLSKNKETIISNDCLPINDDEQTKVEIFDVVGLNYEGRRKELKKLIKKMK
                     NNDDFFFLYSDLKGNELKEELLYEDKVYEIPDYEVIPGVFLQKEPDNPYDENAIKVMI
                     SNEYSEFHVGYVPREYASRLVNHMDNIVSCNAYINGGKYKTLDYLEEKIVTKESDYGL
                     RVHLEYKV"
     gene            complement(1983..2699)
                     /locus_tag="VB241_000333"
     CDS             complement(1983..2699)
                     /locus_tag="VB241_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1243666.1"
                     /translation="MNSFKDRLKQIMSERKISQSELSRRTGIGRNSISDYLNGKYEAK
                     QDKVFELAKALNVNEAWLMGFDISKNRKIENNDITSIYSKLTPPRQSNVLKYATNQLE
                     EQNNDSDNLVDFNSYIQEKSEVDIYGCASAGIGERLYNEPISKEFVRGYVPAHDIALK
                     VNGDSMEPLFKNGQIIFIEKSHTIKDGQIGVFIINGDAYVKKVYVEDNRLTLVSLNKK
                     YKDLYFYDNESVRLVGKVIL"
     gene            2863..3105
                     /locus_tag="VB241_000334"
     CDS             2863..3105
                     /locus_tag="VB241_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HEP1243667.1"
                     /translation="MIYNFDYSLLYERMAEYRYSQSSLANAIPISRTSINHKLQGKNL
                     FTQWEIKRICELLEIPPTKVGRYFFEQNVQKPVQMS"
     gene            3118..3561
                     /locus_tag="VB241_000335"
     CDS             3118..3561
                     /locus_tag="VB241_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243668.1"
                     /translation="MEQITLTKEELKEIIAKEVREAINGKKPISSGSIFSKVRINNDD
                     LEEINKKLNFAKDLSLGRLRKLNHPIPLKKYQHGFESIHQKAYVQDVHDHIRKLTLSI
                     FGVTLNSDLSESEYNLAAKVYREIKNYYLYIYEKRVSELTIDDFE"
     gene            3576..3716
                     /locus_tag="VB241_000336"
     CDS             3576..3716
                     /locus_tag="VB241_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243669.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERNNNPELLRAVAELLKK
                     VS"
     gene            complement(3709..3918)
                     /locus_tag="VB241_000337"
     CDS             complement(3709..3918)
                     /locus_tag="VB241_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243670.1"
                     /translation="MKLLVTLKDGSKKHVSDLKKIVFPGYEGIETVTKEEIETFFLDP
                     TKTYVFVGSQTLSVEAGQILTVEFS"
     gene            3975..4724
                     /locus_tag="VB241_000338"
     CDS             3975..4724
                     /locus_tag="VB241_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEP1243671.1"
                     /translation="MQALKTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            4737..4997
                     /locus_tag="VB241_000339"
     CDS             4737..4997
                     /locus_tag="VB241_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1243672.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDKKINATPIKE"
     gene            5017..5154
                     /locus_tag="VB241_000340"
     CDS             5017..5154
                     /locus_tag="VB241_000340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243673.1"
                     /translation="MFKILNDIKTSLKNHPWGWKEHLPYLLMLTLSLVALIFGVLSAI
                     L"
     gene            5231..5470
                     /locus_tag="VB241_000341"
     CDS             5231..5470
                     /locus_tag="VB241_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243674.1"
                     /translation="MSEPIKEKLELLILKTLKHKENSTSIISNDELEKLFNMYKYLNE
                     PNQKRVWIVDLIPCVALICATSILVTLLLLVMQLL"
     gene            5797..6123
                     /locus_tag="VB241_000342"
     CDS             5797..6123
                     /locus_tag="VB241_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243675.1"
                     /translation="MNIQKVMKLILKKIHEMREILKKFNKNIRHKDLIVIKVKDENSV
                     PLVIYKGGELKSKRVVKFLWVTRNGNYEGGYDINIEHYAKSEKGRPGRYEKSGFRSLF
                     FKEDSQ"
     gene            6120..6338
                     /locus_tag="VB241_000343"
     CDS             6120..6338
                     /locus_tag="VB241_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243676.1"
                     /translation="MNKLYKTTLLITMAVVTWKVWKIEKHTRKPVISSRALSDYLNNK
                     SLTIPKDAENSTESARRLLKFAEQTISK"
     gene            6368..6688
                     /locus_tag="VB241_000344"
     CDS             6368..6688
                     /locus_tag="VB241_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEP1243677.1"
                     /translation="MPHVLNVTVPIPETHVLITKDEYEELIAYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            6685..6846
                     /locus_tag="VB241_000345"
     CDS             6685..6846
                     /locus_tag="VB241_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HEP1243678.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            6939..7199
                     /locus_tag="VB241_000346"
     CDS             6939..7199
                     /locus_tag="VB241_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HEP1243679.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     GINITDENDLYTALDILNQSIYEWIEENTDEQDRLINLVMKW"
     gene            7212..7748
                     /locus_tag="VB241_000347"
     CDS             7212..7748
                     /locus_tag="VB241_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEP1243680.1"
                     /translation="MNELQERELETFEQDDRFKVTDLDSANWVFKKLDAITTKENEIN
                     ELANKEIERISEWKDKEVEKLQSGKEYLQSLVIEYFRTQKEQDSKFKLNTPYGKVTAR
                     KGSKVIQVSNEQEVIKQLEQRGFDNYVKVTKKLSQSDIKKDFNVTENGTLIDANGEVL
                     EGASIVEKPTSYTVKVGE"
     gene            7749..8396
                     /locus_tag="VB241_000348"
     CDS             7749..8396
                     /locus_tag="VB241_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEP1243681.1"
                     /translation="MTEQLNLYQKIADVKANIAGFTKDTKGYNFSYVSGSQILHRIRE
                     KMIEHNLLLVPNTSNENWTTHTFKNKKGQEVTEFIVEMDLNYTWINADKPEEQYEVSY
                     HAYGQQNDISQAHGTALTYAERYFLMKFFNIPTDEDDADAKQKQDKYSAVSQELKDML
                     TKEANDFIAIAKESGFAEKHKEQINKLEKMDVEALNKNQINVTRQQIKKWLGGIE"
     gene            8396..8812
                     /locus_tag="VB241_000349"
     CDS             8396..8812
                     /locus_tag="VB241_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1243682.1"
                     /translation="MNTVNLIGNLVADPELKGQNNNVVNFVIAVQRLFKNKQTNEYET
                     DFIRCVAFGKTAEIIANNFNKGNKIGVTGSLQTGSYENNQGQKVFTTDIAVNNITFIE
                     RKNNGQSNNQQQNRQTQTGNNPFDNTTAITDDDLPF"
     gene            8826..9500
                     /locus_tag="VB241_000350"
     CDS             8826..9500
                     /locus_tag="VB241_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243683.1"
                     /translation="MPIITSYITQDDGTTTVVISGVELGNKETLLLDNGFDVEVDVSV
                     IDPFRITGKQRRKIFALVKDIEEHTGQPMDYMRHMFIEFVRTYYGYDERISLSNCTRT
                     QASQIIEETLDWTFYNDIPLSYKTSNLLKQDKSFLYWSTVNRNCVICGKPHADLAHYE
                     AVGRGMNRNKMNHYDKHVLALCREHHNQQHAIGVKSFDDKYQLHDSWIKVDERLNKML
                     KGENND"
     gene            9493..10224
                     /locus_tag="VB241_000351"
     CDS             9493..10224
                     /locus_tag="VB241_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1243684.1"
                     /translation="MTDQPSYYSIITANVRYDNRLTDSEKLLFAEITSLSNKYGYCTA
                     SNGYFSKLYEVTKRTISARINNLKENGYLKIELDYKGSEVKQRRLYPMTQSSIPIENN
                     FYRGVENNFHTPIERNFQENNTSINITRLNNTSINNNSATDVTHEQFEEWWKLYDKKK
                     DKKMSSTKFKSCLKKHSFEQIMQGTREYLKTITDKQYQKYPKTFLTNESYMNDYSEEI
                     KEEVNNQYVDAFQRASQSSIENLPF"
     gene            10237..11022
                     /locus_tag="VB241_000352"
     CDS             10237..11022
                     /locus_tag="VB241_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1243685.1"
                     /translation="MESFQNLAKKPTLKKQIIEQAFDLKCENCGRKYDYYKFDDGSEF
                     KHGCDCEMIEYAKQSTENYHKRNRRRKAERIFKQSIMNEDLTKATFDNYNPTNNQLEY
                     AKNLCERYANNFTLDNKQSLLIQGSFGTGKSHLSMSIVKSVKAKGYTVLYMNVPQLIS
                     TIKNTYNNQTAMTEQELAQIISDVDLMVFDDYGINMNEFATSKMFELIESRIGKHNIF
                     TTNLDEKEMTKNKDLQRIFSRIMSNTTLIKMDGQDYRTRGLKL"
     gene            11019..11177
                     /locus_tag="VB241_000353"
     CDS             11019..11177
                     /locus_tag="VB241_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243686.1"
                     /translation="MITKEFLKTKLECSDMYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAIVEY"
     gene            11190..11411
                     /locus_tag="VB241_000354"
     CDS             11190..11411
                     /locus_tag="VB241_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HEP1243687.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTRAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            11422..11826
                     /locus_tag="VB241_000355"
     CDS             11422..11826
                     /locus_tag="VB241_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEP1243688.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGANYDRIELQP
                     KFELQPKFGKQRPITYIADFSLWKEGKLVEVVDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            11831..12016
                     /locus_tag="VB241_000356"
     CDS             11831..12016
                     /locus_tag="VB241_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HEP1243689.1"
                     /translation="MQQQAYINATIDIRIPTEVEYHHFDDVDDEKDMLAERLDKNPDE
                     LLKYDDIKIRHAYIEVE"
     gene            12017..12373
                     /locus_tag="VB241_000357"
     CDS             12017..12373
                     /locus_tag="VB241_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243690.1"
                     /translation="MSIVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LNAPYGMRLAEYKEIVLSKIMERESKEREMARQPRKEAELRRKKPHLFNVPQKHSRGR
                     YACYLMENDIFVKVKK"
     gene            12377..12619
                     /locus_tag="VB241_000358"
     CDS             12377..12619
                     /locus_tag="VB241_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243691.1"
                     /translation="MTDSARKEYLSRFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQDLEYEEQKQLTLF"
     gene            12628..12999
                     /locus_tag="VB241_000359"
     CDS             12628..12999
                     /locus_tag="VB241_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP1243692.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTVKNELLGRYEHYCFKIRELHPESKANRIGALYIGGKSTADIILSRME
                     ELDGTNEFYEFLGQMEDDTNE"
     gene            12992..13240
                     /locus_tag="VB241_000360"
     CDS             12992..13240
                     /locus_tag="VB241_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HEP1243693.1"
                     /translation="MNNREQIEQSVISTSAYNGNDTEGLLKEIEDVYKKARAFDEILE
                     SLPNAMQDALKEDIYLDEAVGIMTSQVVYKYEEAQEND"
     gene            13233..13763
                     /locus_tag="VB241_000361"
     CDS             13233..13763
                     /locus_tag="VB241_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HEP1243694.1"
                     /translation="MTNTLQVKLLSKDARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTYLVIETGKIDAGYHGNLGINIKNDMEHDGIT
                     SLYEDLDDKLVNTLDIKGNYINEGEGARKVYKINKGDKLAQLVIVPIWTPELKQVEEF
                     ESVSERGAKGFGSSGV"
     gene            13800..14045
                     /locus_tag="VB241_000362"
     CDS             13800..14045
                     /locus_tag="VB241_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243695.1"
                     /translation="MSYIITLVPILVFIVIFNNLLNRYMVLYKELDLFTCRIGMLLVL
                     IVIVDFAKQKNMLATLSVVLILLFVEKLRIIQRSDKK"
     gene            14042..14248
                     /locus_tag="VB241_000363"
     CDS             14042..14248
                     /locus_tag="VB241_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HEP1243696.1"
                     /translation="MTQYLVTTFKDSTGLPHEHITVARDNQTFTVVEAENKEEAKEKY
                     EAQVKRDAIIKLGQLFENIRECGK"
     gene            14245..14394
                     /locus_tag="VB241_000364"
     CDS             14245..14394
                     /locus_tag="VB241_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1243697.1"
                     /translation="MIKEILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            14394..14594
                     /locus_tag="VB241_000365"
     CDS             14394..14594
                     /locus_tag="VB241_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HEP1243698.1"
                     /translation="MWITMTIVFAILLLVCISINSDHAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIELKRINNDIKK"
     gene            14622..15038
                     /locus_tag="VB241_000366"
     CDS             14622..15038
                     /locus_tag="VB241_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1243699.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            15270..15569
                     /locus_tag="VB241_000367"
     CDS             15270..15569
                     /locus_tag="VB241_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEP1243700.1"
                     /translation="MMTKDERIRFYKSKEWQITRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            15700..16044
                     /locus_tag="VB241_000368"
     CDS             15700..16044
                     /locus_tag="VB241_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243701.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            16041..17702
                     /locus_tag="VB241_000369"
     CDS             16041..17702
                     /locus_tag="VB241_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEP1243702.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQTHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKIKPDGNK
                     EYIKKDEVRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            17718..18905
                     /locus_tag="VB241_000370"
     CDS             17718..18905
                     /locus_tag="VB241_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP1243703.1"
                     /translation="MSILEKIFKTRKDISYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            18889..19626
                     /locus_tag="VB241_000371"
     CDS             18889..19626
                     /locus_tag="VB241_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEP1243704.1"
                     /translation="MKVEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASDTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            19649..20794
                     /locus_tag="VB241_000372"
     CDS             19649..20794
                     /locus_tag="VB241_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEP1243705.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEDTEETL"
     gene            20814..21098
                     /locus_tag="VB241_000373"
     CDS             20814..21098
                     /locus_tag="VB241_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243706.1"
                     /translation="MVKFKVVREFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIIDLLNGEDNDD"
     gene            21088..21372
                     /locus_tag="VB241_000374"
     CDS             21088..21372
                     /locus_tag="VB241_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEP1243707.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            21356..21718
                     /locus_tag="VB241_000375"
     CDS             21356..21718
                     /locus_tag="VB241_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEP1243708.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            21715..22119
                     /locus_tag="VB241_000376"
     CDS             21715..22119
                     /locus_tag="VB241_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP1243709.1"
                     /translation="MSVKVTGDKALERELEKHFGIKEMVKVQDKALIAGAKVIVEEIK
                     KQLKPSEDSGALISEIGRTEPEWIKGKRTVTIRWRGPFERFRIVHLIENGHVEKKSGK
                     FVKPKAMGGINRAIRQGQNKYFETLKRELKKL"
     gene            22116..22523
                     /locus_tag="VB241_000377"
     CDS             22116..22523
                     /locus_tag="VB241_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243710.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            22524..23168
                     /locus_tag="VB241_000378"
     CDS             22524..23168
                     /locus_tag="VB241_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1243711.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHGGDGEKGEEAFLKKILGEEYTGNVTEDNEETL"
     gene            23210..23434
                     /locus_tag="VB241_000379"
     CDS             23210..23434
                     /locus_tag="VB241_000379"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEP1243712.1"
                     /translation="MPDSIKTLKVGDTYDLNIVVEPSNQSKLLKYTTDQTNIVSINRD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            23484..23834
                     /locus_tag="VB241_000380"
     CDS             23484..23834
                     /locus_tag="VB241_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243713.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            24079..28608
                     /locus_tag="VB241_000381"
     CDS             24079..28608
                     /locus_tag="VB241_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEP1243714.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSILDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQL
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNSVTVLAPLLASITKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAIESVKQTFSNFIQFIQPFIDSVKNIFKQAISAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNTVGIAGRLWDKVRNIFGNMRDGLKSIIGKIKDHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIAHDTFATVGDKGRGNGPNGFRNEMI
                     EYPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDKIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWVIPTDPARRNDAMKMLHYAAAEVRGKNASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            28605..30089
                     /locus_tag="VB241_000382"
     CDS             28605..30089
                     /locus_tag="VB241_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP1243715.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNNGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHTEFRDFKGWNKMTAEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKVYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            30105..33890
                     /locus_tag="VB241_000383"
     CDS             30105..33890
                     /locus_tag="VB241_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243716.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECVASYLADITTAKPYAPG
                     KLEKKTTSEALKEVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMALD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFEHKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTQALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            33880..34032
                     /locus_tag="VB241_000384"
     CDS             33880..34032
                     /locus_tag="VB241_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243717.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            34079..34366
                     /locus_tag="VB241_000385"
     CDS             34079..34366
                     /locus_tag="VB241_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243718.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVDRTDLSSDKTVDSETM"
     gene            34422..34796
                     /locus_tag="VB241_000386"
     CDS             34422..34796
                     /locus_tag="VB241_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243719.1"
                     /translation="MDDIQKIKKELSELVERVDDVEVLANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVGENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
     gene            35217..35990
                     /gene="sea"
                     /locus_tag="VB241_000387"
     CDS             35217..35990
                     /gene="sea"
                     /locus_tag="VB241_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type A"
                     /protein_id="HEP1243720.1"
                     /translation="MKKTAFILLLFIALTWTTSPLVNGSEKSEEINEKDLRKKSELQG
                     TALGNLKQIYYYNEKAKTENKESHDQFLQHTILFKGFFTNHSWYNDLLVDFDSKDIVD
                     KYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWLDGKQN
                     TVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVFHTSTEPSV
                     NYDLFGAQGQNSNTLLRIYRDNKTINSENMHIDIYLYTS"
     gene            36099..36275
                     /locus_tag="VB241_000388"
     CDS             36099..36275
                     /locus_tag="VB241_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP1243721.1"
                     /translation="MDRWWLSEYKEVVPMVALHKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(36328..36435)
                     /locus_tag="VB241_000389"
     CDS             complement(36328..36435)
                     /locus_tag="VB241_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243722.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            36487..36741
                     /locus_tag="VB241_000390"
     CDS             36487..36741
                     /locus_tag="VB241_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP1243723.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            36753..37508
                     /locus_tag="VB241_000391"
     CDS             36753..37508
                     /locus_tag="VB241_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1243724.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            37699..38190
                     /gene="sak"
                     /locus_tag="VB241_000392"
     CDS             37699..38190
                     /gene="sak"
                     /locus_tag="VB241_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HEP1243725.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPRYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVVI
                     EKK"
     gene            38794..39132
                     /locus_tag="VB241_000393"
                     /pseudo
     CDS             38794..39132
                     /locus_tag="VB241_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            39643..39993
                     /gene="scn"
                     /locus_tag="VB241_000394"
     CDS             39643..39993
                     /gene="scn"
                     /locus_tag="VB241_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HEP1243726.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGLKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            complement(40046..40306)
                     /locus_tag="VB241_000395"
     CDS             complement(40046..40306)
                     /locus_tag="VB241_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243727.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
BASE COUNT        15194 a         5804 c         7882 g        11753 t
ORIGIN      
        1 ttttaatata gctgaattat tttgcgcgct aattaaattt aaaaacaatc ttaaataata
       61 ctcccatttc aaatcaaaat tcatctttaa atactttttg ttttctttag aagataaggg
      121 aataacattt actatatctt ccgtattaga atcattttta ttcatcacta ttgcaaagtg
      181 tgaattagag aattctttat taacgtttat accgaaatct acaaaaacta tttctccttg
      241 tttaaacttt ggataaaaac ctttatggtt tttttcacct tcaaatctct tgagtaaata
      301 gtgaatatct gaatctaatt ttttaaattt tggatttcca gaagttttta atttattaat
      361 gcgtttttct atattatgcg tcatcatttc tcctttattc tcgctcacac tctcaccacc
      421 attcaacgtc tacacttgta gacgtttttt gattagtaaa atcataatga atcttctttg
      481 gttaacttat cgccatctat tttttgtgaa ataaattcca agtatttacg cgcattatgt
      541 gacgataaat ctttaggtaa ctcataagtg aatggttgat taccactagt taaaacttca
      601 tatactatag tttctttttt tattttgcaa ttttttattt tcattataaa cttcctttca
      661 aacactgctg aaatagacgt cttttatatt aaagcgccac acaggcgctg ttaatcacaa
      721 tttagttcta tcagtgattt tagactccat aactctttga tgtgattctt tagcttctcg
      781 aatcatatct ttaaattctt gactgtctat aaaagcttta gcctcttcta tttgctcttg
      841 agtaagctct ttaccaccag tattgatgtg taagtgttca atttctttat aagtactcat
      901 tttttcgact cctgttcttc aagttcactt ttagttatag gtaaaccatt gttcaatcta
      961 taagtcagtt cttcttctgt ataaaagggg atttcaacca tttcccactc ttcaatgtta
     1021 atgtcaactt ctttaaaatc catgttaaac cctcctgtga aatgaatttt ctattattta
     1081 tagtaattac ttataaaaat acaatcttta cctatctcaa actttgtatt ctaaatgtac
     1141 tcgtaatcca tagtctgatt ctttagtaac gattttctct tctaaataat ctaaagtttt
     1201 atacttacca ccattaatat atgcgttaca agaaacgatg ttgtccatat gattgactaa
     1261 tcttgaagca tactctctag gtacatatcc aacgtgaaat tcagagtatt catttgaaat
     1321 cataactttt atcgcgtttt catcataagg attatccggt tctttttgta agaatacacc
     1381 aggaataacc tcgtaatcag gaatttcata caccttgtct tcataaagta attcttcttt
     1441 aagttcattt cctttcaaat cactatataa gaaaaagaaa tcgtcgttat ttttcatttt
     1501 cttgataagt ttctttaatt cttttctacg accttcataa tttaatccta cgacgtcgaa
     1561 aatttcaact ttagtttgtt catcatcatt aataggtaga caatcattcg agataattgt
     1621 ttccttattc ttagataatt gcatataagt ttttaaaatt gagatgaatc ctgttaaagg
     1681 agagtttgtt acgaaataaa ctgttaattt tctattatcg tttaatgtta aaaaagcttg
     1741 gtttttccaa atagtaacaa cagtgttata atctatcacc tctgataatg agattttgaa
     1801 tatataatct tcttctttcc ttataaaaca aatctcttca tgtgaaatga atatagaacc
     1861 cattctcctc ttgttttcgt cgaattttat gtcgcaactg tcgctgatta ttggttcaaa
     1921 gtaactgtat tgatctgata atattttttc atcttgcttt ctaggtttca ttttactacc
     1981 tcctataaaa taacttttcc aactaacctc acactttcgt tatcataaaa atataaatct
     2041 ttatactttt tatttaaaga aaccaacgtt aatctattat cttctacata aactttcttt
     2101 acgtaagcat ctccatttat aataaagacg cctatttgtc catctttgat agtgtgagat
     2161 ttttcaatga atataatttg tccgttttta aataacggct ccattgagtc tccatttact
     2221 tttaaagcta tatcatgtgc ggggacataa cctcttacga attcttttga aataggctcg
     2281 ttatataatc tttcgccaat accagctgac gcacaaccat atatatccac ttcggatttt
     2341 tcttgaatgt aagaattgaa atctaccaga ttatcactgt cattattttg ttcttctaat
     2401 tgattagtcg catattttag tacattgctt tgtcttggag gcgtgagttt actgtatatg
     2461 gaagtgatgt cgttattttc aatttttcta ttcttagaaa tatcaaaccc cataagccac
     2521 gcttcgttaa cgtttaaagc ctttgctagt tcaaagactt tgtcttgttt cgcttcatat
     2581 tttccgttta aataatcgct aattgagttt ctaccaatac cagtccttct tgatagctct
     2641 gattgagata tcttccgttc agacataatt tgctttaatc tatccttaaa actgttcata
     2701 tttctgaaca cctcctaaga acataatact acgtacaatg acgattatca ataattttta
     2761 acaaatattg tacagaaaaa tgtattttat gtgttgactt atttaaacaa aggtgtttta
     2821 attgatttgt acagaaaacc gaacaagaag ggaggtgagt ttatgatata caatttcgat
     2881 tatagtttgc tgtacgaaag aatggcagag tatagatata gccaaagctc tttagcgaac
     2941 gcaatcccta tttcaaggac atctattaat cacaagttgc aaggaaaaaa tttatttaca
     3001 caatgggaaa taaaacgaat ctgtgaatta ttagaaatcc caccaacaaa agtaggtaga
     3061 tatttttttg aacaaaatgt acagaaacct gtacaaatgt cgtaacagga ggaaactatg
     3121 gaacaaatca cattaaccaa agaagagttg aaagaaatta tagcaaaaga agttagagag
     3181 gctataaatg gcaagaaacc aatcagttca ggttcaattt tcagtaaagt aagaatcaat
     3241 aatgacgatt tagaagaaat caataaaaaa ctcaatttcg caaaagattt gtcgctagga
     3301 agattgagga agctcaatca tccgattccg ctaaaaaagt atcagcatgg cttcgaatca
     3361 attcatcaaa aagcttatgt acaagatgtt catgaccata ttagaaaatt aacattatca
     3421 atttttggag tgacacttaa ttcagacttg agtgaaagtg aatacaacct agcagcaaaa
     3481 gtttatcgag aaatcaaaaa ctattattta tacatctatg aaaagagagt ttcagaatta
     3541 actatcgatg atttcgaata aaggaggaac aacaaatgtt acaaaaattt agaattgcga
     3601 aagaaaaaaa taaattaaaa ctcaaattac tcaagcatgc tagttactgt ttagaaagaa
     3661 acaacaaccc tgaactgttg cgagcagttg cagagttgtt gaaaaaggtt agctaaattc
     3721 aacggtaagg atttgccctg cctccacact tagagtttga gatccaacaa acacataagt
     3781 tttagtaggg tctagaaaaa atgtttcgat ttcctctttt gtaacagttt caattccttc
     3841 atatcctgga aaaacaattt tctttaaatc cgaaacatgt ttttttgaac catcctttaa
     3901 agtaactaga agtttcatac ttatcacctc cttaggttga taacaacatt atacacgaaa
     3961 ggagcataaa caatatgcaa gcattaaaaa caaaatcgaa catcggcgaa atgttcaaca
     4021 tacaagaaaa agaaaatgga gaaatcgcaa taagtgcaag agagttatat aaagctttgg
     4081 aagttaaaaa gcgttttagc gcttgggcag aaattaactt gaagcatttc aaagaaaata
     4141 gggattttac aagtgtactt acaagtacgg ttgttaataa cggagctgta agacaactag
     4201 aagattatgc tttaacactt gatgtagcta aacatgttgc gatgatgtca ggtacagaaa
     4261 aaggttttga ttttagagag tatttcatcc aagtagagaa agcatggaac agtccagaaa
     4321 tgattatgca acgtgcttta aaaattgcta acaacacaat caatcaatta gaaacaaaga
     4381 ttgaacgtga taaaccaaaa attgtatttg cagatgcagt agctactact aagacatcaa
     4441 ttttagttgg agagttagca aagatcatta aacaaaacgg tataaacatc gggcaacgca
     4501 gattgtttga gtggttacgt caaaacggat tccttattaa acgcaagggt gtggattata
     4561 acatgcctac acagtattca atggaacgtg agttattcga aattaaagaa acatcaatca
     4621 cacattcgga cggtcacaca tcaattagta agacgccaaa agtaacaggc aaaggacaac
     4681 aatactttgt taataagttt ttaggagaaa aacaaacatc ttaataggag aacactatgg
     4741 aacaaatcac attaaccaaa gaagagtgtg tcgaacaatg catcaataaa gacttaaaac
     4801 ttttagatta tcgagttcaa caaattttag aaggtgttct atcagaaagt accacatacg
     4861 gtgatgcaag aaataaatta gaaacattga aaattattgc tgaatctcat tttaaaaccg
     4921 aacatgcttc agttatttac aaattagcat tgaaaaagtt agacaaaaaa atcaacgcca
     4981 ctccaattaa agagtgacga aaaaggagga tttcaaatgt ttaagatttt aaatgatata
     5041 aaaacttctt taaaaaacca tccttggggt tggaaagagc acttacctta tttactgatg
     5101 ttaactctgt cgcttgtggc tctgattttc ggtgttctgt ccgcgattct atgataacag
     5161 gttttatata gattccttac ctcctctctg taggagataa caatattata cacgaaagga
     5221 gatgtaacaa atgagtgaac caatcaaaga aaagttagaa ctactaattc ttaaaacact
     5281 taaacataaa gaaaattcaa catctatcat cagtaatgac gagttagaaa agctatttaa
     5341 tatgtataaa tatttaaatg agcctaatca aaaaagggtt tggatagtag atttaatccc
     5401 atgcgtagct ttgatttgtg caactagcat tctagtaacg cttttattac tagtgatgca
     5461 attgctatag aaatactgat gattgtacca agaatccatc taatccaact gttacgcgcg
     5521 gaaaaataaa catctttacc tttagatgtt atagacacgt aaccactgta tttcatatac
     5581 agaatcgaga atgaagaatc ttcagaaatt tcgaggtcgt tctcaacttg agtaatccat
     5641 tctttgcgaa gcatgtattc aaagtcttta tgctgatcag atagttttat tttttctttg
     5701 ctacaggctt tatgtagtac taaaaaagtt gagatattca cacacatcac ctctttaggt
     5761 tgataactaa attatacacg aaaagagatg taacaaatga acattcaaaa agtaatgaaa
     5821 ttaatattga aaaaaattca tgagatgagg gagattttaa aaaagttcaa caaaaatatc
     5881 agacacaaag atctaattgt catcaaagtg aaagatgaaa acagcgttcc attagtcatt
     5941 tataaaggtg gagagctgaa gagcaaacga gtagttaaat ttttatgggt aactagaaac
     6001 ggaaattacg aaggtggtta cgacataaac atagagcatt atgcaaagag tgaaaaaggc
     6061 agacccggta gatatgaaaa atcaggattt agaagtttgt tttttaagga ggattcacag
     6121 tgaacaaatt gtataaaaca accctcctca tcacaatggc agttgtgacg tggaaggttt
     6181 ggaagattga gaagcacact agaaaacctg tgattagtag cagggcgttg agtgactatc
     6241 taaacaacaa atctttaacc ataccgaaag atgctgaaaa ttctactgaa tctgctcgtc
     6301 gccttttgaa gttcgccgaa caaactatta gcaaataaca acattataca cgaaaggaat
     6361 gatagaaatg ccacatgtat taaacgtaac cgttccaata cctgaaacac acgtgcttat
     6421 cacaaaagat gaatatgaag agttaatagc ttactcatta gaccctgtat ggaacatgag
     6481 cgacttaaag aagaaattaa aaattgcatc tgatgagact atcaaggaca gattactatt
     6541 tcatcctaga tttgaaaaag aactaagagc gcaaggaatt gtgcattacc cagatgagaa
     6601 ttttaatcgc tggagattta acgcaagaaa gatgaataaa ttcgtcgatg agcatttcaa
     6661 tgaaatatat aaggagagaa taaaatgagc aacatttata aaagctacct attagcagta
     6721 ttatgcttca cagtcttggc aattgtactt atgccgtttc tatacttcac cactgcatgg
     6781 tcgattgcgg gatttgcaag tatcgcaaca ttcatatttt ataaggaata cttttatgaa
     6841 gaataaaaaa actgttactc acggcaatga gtaacagtct aaacaattag aaaattaatg
     6901 catattcaat ataaaacgaa ataaaggaag tgtcaacaat gtactacaaa attggcgatg
     6961 tatgtcaaaa agtaattaat gtagacggat tcgattttaa attagcagtt aagaaacaag
     7021 attacagcat tctagtgaat gtcttagatt tagaagatag atttatcgac ggtataaata
     7081 taacagatga gaatgatcta tacacagcat tagacatatt aaatcaatct atttatgaat
     7141 ggattgaaga gaacacagac gagcaggaca gactaattaa cttagtcatg aaatggtagg
     7201 aggcatgaaa agtgaatgaa ttacaagaga gagaattaga aacattcgaa caagacgacc
     7261 gattcaaagt aactgatcta gacagtgcta actgggtttt taagaaactg gatgcaatca
     7321 caactaaaga gaacgaaatc aacgagttag caaataaaga aattgaacgt ataagcgaat
     7381 ggaaagataa agaagtagaa aaattacaga gtggcaaaga atatttacaa agtcttgtaa
     7441 ttgaatattt cagaacacaa aaagaacaag atagcaaatt caagttgaac acaccttacg
     7501 gaaaagtgac agccagaaaa ggttcaaaag tcattcaagt tagcaatgag caagaagtca
     7561 ttaaacaact tgagcaacga ggttttgaca actatgtaaa agtaactaaa aaacttagcc
     7621 aatcagacat taagaaagat ttcaatgtaa ctgaaaacgg cactttgatt gacgcaaacg
     7681 gcgaagtttt agagggtgct agcatcgttg agaaaccaac gtcatacacg gtaaaggtgg
     7741 gagaatagat gactgaacaa cttaatttat accaaaaaat agcagatgtt aaagcgaata
     7801 ttgcgggatt cacaaaagat actaagggat ataacttttc gtatgtttca ggatctcaaa
     7861 tattacacag aataagagaa aagatgattg aacataattt attgttagtc cctaatacgt
     7921 caaatgaaaa ttggacgaca catactttta aaaacaaaaa aggtcaagaa gtgacagaat
     7981 tcatagttga aatggatttg aattatacat ggattaatgc cgataaacca gaagaacagt
     8041 atgaagttag ttatcatgct tacggtcaac aaaatgatat ttcacaagca catggaacag
     8101 cgttaactta tgctgaacgc tatttcttaa tgaagttctt taacatccca actgatgaag
     8161 atgacgcaga cgcaaaacaa aaacaagata aatattcagc agtaagtcaa gaacttaaag
     8221 acatgctaac taaagaagct aatgatttta tagccatagc taaagaaagt ggattcgcgg
     8281 aaaaacacaa ggaacaaatt aacaaattag aaaaaatgga cgtcgaagca ctgaacaaaa
     8341 accaaatcaa tgtaaccaga caacagataa aaaaatggct tggaggaatt gaataatgaa
     8401 cacagtaaat ttaattggga acctagtggc agatccagag ttaaaaggtc aaaacaacaa
     8461 cgtagttaac tttgtaattg cagtacagag attattcaaa aataaacaaa cgaatgaata
     8521 tgaaacagac ttcattcgtt gtgttgcatt tggtaagact gctgaaatca tcgctaataa
     8581 ctttaataaa ggtaataaaa ttggcgttac tggttcatta caaaccggta gttatgaaaa
     8641 taatcaaggg cagaaagttt ttactacaga catcgcagta aacaatataa ctttcattga
     8701 acgtaaaaat aacggtcaat ctaacaacca acaacaaaac agacaaactc aaactggtaa
     8761 taatcctttt gataatacca ccgccattac tgatgatgac ctcccattct gattggaatg
     8821 attagatgcc aataattact agttatatca ctcaagatga cggtacaaca acagttgtca
     8881 tctcgggtgt cgaattaggc aataaagaaa cattactact tgataacggt tttgatgtag
     8941 aagtcgatgt aagcgtcata gatccgtttc gaattaccgg caagcaacga cgaaaaatat
     9001 tcgcgcttgt caaagacata gaagaacata caggtcaacc aatggactat atgcgacata
     9061 tgttcatcga atttgtaaga acgtactacg gctatgatga acgcatttcg cttagtaatt
     9121 gtacgagaac acaagcgagt caaatcattg aagaaacgct tgactggacg ttctacaacg
     9181 acataccact tagctacaaa acgagtaatc tactgaaaca agataaatca ttcttatact
     9241 ggtcaactgt taaccgcaac tgtgtaatat gcggaaagcc tcacgctgac ctagcgcatt
     9301 atgaagcagt cggcagaggc atgaacagaa acaagatgaa tcactacgac aaacatgtat
     9361 tagcgttatg tcgcgaacat cataaccagc aacatgcgat tggtgttaag tcatttgatg
     9421 ataaatatca attgcatgac tcgtggataa aagttgatga gaggctcaac aaaatgctga
     9481 aaggagagaa caatgactga tcaaccaagt tactactcaa taataacggc aaatgtcaga
     9541 tacgataacc gacttaccga cagtgaaaaa ctactttttg cagaaataac gtctttaagt
     9601 aacaagtacg gatattgcac agcaagtaat ggttactttt ccaaattgta tgaagtaacg
     9661 aaaagaacta tatcagccag gataaacaac ttaaaagaaa atggttattt aaaaattgag
     9721 cttgattata aagggagtga agttaaacaa agaaggttat accccatgac gcaatcttct
     9781 atacctatag aaaataattt ctataggggt gtagaaaata atttccatac ccctatagaa
     9841 agaaacttcc aagagaataa tacaagtatt aatattacaa gattaaataa tacaagtatt
     9901 aataataata gcgcaactga cgttacgcat gagcaatttg aggaatggtg gaaactttat
     9961 gacaagaaga aagataagaa gatgtcttcc actaaattca aatcatgctt aaagaaacat
    10021 tcttttgaac aaatcatgca aggtactaga gaatatttaa aaactattac agacaaacaa
    10081 tatcaaaagt accctaaaac gtttttaact aacgaaagct atatgaatga ttatagcgaa
    10141 gagattaaag aagaagtaaa caatcaatat gtagatgcgt ttcagcgtgc atcacaatca
    10201 agtatagaaa atttaccgtt ttaaaggagt gagaaagtgg agtcattcca gaacttagca
    10261 aagaaaccaa ctttgaagaa acaaatcatt gaacaagcgt ttgatttgaa atgcgagaac
    10321 tgtggacgta agtacgacta ttacaaattt gatgacggtt cagaattcaa acatggttgt
    10381 gactgcgaaa tgatagagta tgccaaacaa tcaactgaaa actatcacaa gagaaacagg
    10441 cggagaaaag cagaacgtat attcaagcaa tcgataatga acgaagatct aacgaaagca
    10501 acgtttgata attacaatcc aactaacaac caactagagt atgcaaaaaa cttatgcgaa
    10561 cgttatgcaa acaatttcac gttagacaat aaacaatcgc tactaattca aggctcattc
    10621 ggtacaggta aatcacacct atcaatgagc attgttaaat cagttaaagc taaaggttac
    10681 acagtgctat atatgaacgt acctcaattg atatcaacga taaaaaatac ttataacaac
    10741 caaactgcta tgaccgaaca ggaattggct caaattataa gtgatgtcga tttaatggta
    10801 ttcgatgact acggtatcaa catgaatgaa ttcgctacta gcaagatgtt cgagcttatt
    10861 gaaagtagga taggcaaaca caacatcttt actactaact tagatgagaa agaaatgaca
    10921 aaaaacaaag acttacaacg tatattcagc agaatcatga gcaatacaac actaatcaag
    10981 atggacggtc aagattacag aactagaggt ttaaaactat gattaccaaa gaatttttaa
    11041 aaactaaact tgagtgttca gatatgtacg ctcagaaact catagacgag gcgcagggcg
    11101 atgaaaatag gttgtacgac ctatttatcc aaaaacttgc agaacgtcat acacgccccg
    11161 ctatcgtcga atattaagga gtgttaaaaa tgccgaaaga aaaatattac ttataccgag
    11221 aagatggcac agaagatatt aaggtcatca agtataaaga caacgtaaat gaagtttatt
    11281 cgctcacaag agcccatttc agcgacgaaa agaaaattat gactgatagt gacctaaaac
    11341 gattcaaagg cgctcacggg cttctatatg agcaagagct aggattacaa gcaacgatat
    11401 ttgatattta gaggtggcac aatgagtaaa tacaacgcta agaaagttga gtacaaagga
    11461 attgtatttg atagcaaagt ggaatgtgaa tattaccaat atttagaaag taatatgaat
    11521 ggcgctaact atgatcgtat cgaactacaa ccgaaattcg aattacaacc taaatttggg
    11581 aagcaaagac cgattacgta tatagccgat ttctctttgt ggaaggaagg gaaactggtt
    11641 gaagttgtag acgttaaagg taaggcgacc gaagttgcca acatcaaagc gaagatattc
    11701 agatatcagt atagagatgt gaatttaaca tggatatgta aagcgcctaa atacacaggt
    11761 caagaatgga tggtatatga ggacttagtg aaagtcagac gtaaaagaaa aagagaaatg
    11821 aagtgattta atgcaacaac aagcatatat aaatgcaacg attgatataa gaatacctac
    11881 agaagttgaa tatcatcatt tcgatgatgt ggatgatgaa aaagatatgc tagcagagcg
    11941 tttagataaa aatccagatg agttattgaa gtatgacgac ataaaaataa gacatgcata
    12001 tatagaggtg gaataaatga gtatcgtaaa gattaacggt aaaccatata aatttaccga
    12061 acatgaaaat gaattgataa aaaagaatgg tttaactcca ggaatggttg caaaaagagt
    12121 acgaggtggc tgggcgttgt tagaagcttt aaacgcacct tatggcatgc gcctagctga
    12181 gtataaagaa atcgtgttat ccaaaatcat ggagcgagag agcaaagagc gtgaaatggc
    12241 taggcaacca cgtaaagaag ctgagctaag aagaaagaag ccacatttgt ttaatgttcc
    12301 tcagaaacat tcaagaggac gttatgcgtg ctacctgatg gaaaacgaca tattcgtgaa
    12361 agttaagaag tagaccatga cagatagcgc acgcaaagaa tacttaagcc gatttttcgg
    12421 ctctaagaga tatctgtatc aggataacga acgagtggca catatccatg tagtaaacgg
    12481 cacttattac tttcacgggc atatcgtacc aggctggcaa ggcgtgaaaa agacatttga
    12541 tacagctgaa gagcttgaaa catatataaa gcaacaggat ttggaatatg aggaacagaa
    12601 gcaactaact ttattttaga ggaggttatg aaagtgaact atgaaacagg attccaaata
    12661 ggcgtaatgg aagctaggtt gaagaagatg agaaaacaac gtgatgagta caagaagcaa
    12721 cgtgacgagc ttattgggga tatagctaag ttaagagagc gtaacgaaga gctggagaac
    12781 atgtggcgca cagtcaaaaa tgaattgctt ggaagatacg aacattactg ttttaaaatt
    12841 agagaactac accctgagag caaagcgaac aggataggag ctctctatat aggaggtaaa
    12901 agcactgcag atattatact gtcgcgaatg gaagaactag acggaacaaa tgagttctac
    12961 gaatttttag ggcaaatgga ggatgacaca aatgaataac cgcgaacaaa tcgaacagtc
    13021 cgttataagt actagtgcgt ataacggtaa tgacacagag ggattgctaa aagagattga
    13081 ggacgtgtat aagaaagcgc gagcgtttga tgaaatactt gagagtttac ctaatgctat
    13141 gcaagatgca ctcaaagaag atatttatct tgatgaagca gtagggatta tgacgagtca
    13201 agtggtctat aaatatgagg aggcgcagga aaatgactaa cacattacaa gtgaaactat
    13261 tatcaaaaga cgctagaatg cccgaacgaa atcataagac ggatgcaggt tatgacatat
    13321 tctcagctga aaccgtcgta ctcgagccac aagaaaaggc agtgattaaa acagatgtag
    13381 ctgtgagtat accagagggc tatgtcggac tattaactag tcgtagtggt gtaagtagta
    13441 aaacgtattt agtgattgaa acaggcaaga tagacgcggg atatcatggc aatttaggga
    13501 ttaatatcaa gaatgatatg gagcatgacg gcataacatc attatacgaa gatttagacg
    13561 acaaactagt aaatacttta gatataaaag gtaattatat aaacgaagga gaaggcgcta
    13621 gaaaggtata taaaatcaac aaaggcgaca aactagctca attggttatc gtgcctatat
    13681 ggacaccgga actaaagcaa gtggaggaat tcgagagtgt ttcagaacgt ggagcaaaag
    13741 gcttcggaag tagcggagtg taaagacatc ttagatcgag tcaaggaggt tttgggaaag
    13801 tgagttacat cattacatta gttccaatac ttgtattcat agtaatattt aacaatttac
    13861 tcaacagata tatggttttg tacaaagaat tagatttatt tacatgcaga attggaatgt
    13921 tattagtctt aatcgttata gtagattttg caaagcaaaa aaatatgttg gctacattga
    13981 gtgttgtact aatactttta ttcgtagaaa aacttagaat cattcaaagg agtgataaga
    14041 agtgacacaa tacttagtaa caacattcaa agattcaaca ggactaccac atgaacatat
    14101 tactgtggct agagataatc agacgtttac agttgttgag gcagagaata aagaagaagc
    14161 aaaagagaag tacgaggcgc aagttaaaag ggatgcgatt attaaattag gtcagttgtt
    14221 tgaaaatata agggagtgtg ggaaatgatt aaagaaatac taagactatt attcttacta
    14281 gcgatgtatg agctaggtaa gtatgtaact gagcaagtat atattatgat gacggctaat
    14341 gatgatgtag aggcgccgag tgattacgtc tttcgagcgg aggtaagtga gtgatgtgga
    14401 ttactatgac tattgtattt gctatattgc tattagtttg tatcagtatt aatagtgatc
    14461 atgcaagaga gatacaagca ctcagatata tgaatgatta tctacttgat gaagtagtta
    14521 aaactaaagg atacaacggg ttagaagaat acaggattga attgaagcga ataaataacg
    14581 atattaaaaa gtaatttata ttatcggagg tattgcattg aatgataaag attgagaaac
    14641 acgatatcaa aaagcttgaa gaatacattc agcacatcga taactatcga agagagttga
    14701 agatgcgaga atatgaatta cttgaaagtc atgaaccaga taatgcggga gctggcaaaa
    14761 gtaatttgcc gggtaatccg attgaacgat gtgcaataaa gaagtttagt gataacaggt
    14821 acaatacatt aagaaatata gttaacggtg tagatagatt gatagatgaa agtgatgagg
    14881 atacgcttga gttattaagg tttagatatt gggattgtcc tattggttgt tatgaatggg
    14941 aagatatagc acattacttt ggtacaagta agacaagtat attacgtaga aggaatgcac
    15001 tgatcgataa gttagcaaag tatattggtt atgtgtagcg gacttttacc ctatgtaagt
    15061 ccgcattaaa acagtttatt atgttagtat cagattaata tttaaagtta ttaactgcta
    15121 atacgacgca tgaacaagag gcgcatcact atgtgatgtg tctttttatt tatgaggtat
    15181 gaacatgttc aaactaatag taaatacatt actacacatc aagtatagat gcgtcttgat
    15241 actacttaag ttatataagg tgaaacatta tgatgactaa agacgaacgt atacgattct
    15301 ataagtctaa agaatggcaa ataacaagaa aaagagtgct agaaagagat aattatgaat
    15361 gtcaacaatg taagagagac ggcaagttaa cgacatatga caaaagcaag cgtaagtcgt
    15421 tggatgtaga tcatatatta tcgctagaac atcatccgga gtttgctcat gacttaaaca
    15481 atttagaaac actgtgtatt aaatgtcaca acaaaaaaga aaagagattt ataaaaaaag
    15541 aaaataaatg gaaagacgaa aaatggtaaa tacccccggg tcaaaaaaat caaaagcgat
    15601 caaaatactt ggggaacggt tagggagtaa acttcgcgat aattttaaaa atccatgtac
    15661 acccccccct cttataacca ttttaaggca ggtgatgaaa tggagattat agttgatgaa
    15721 aacttagtgc ttaaagaaaa agaaaggctg caagtattat ataaagacat acctagcaat
    15781 aaattaaaag tagttgatgg tttaattatt caagcagcaa ggctacgtgt aatgcttgat
    15841 tacatgtggg aagacataaa agaaaaaggt gactatgatt tatttactca atctgaaaag
    15901 gcgccaccat atgaaagaga aagaccagta gctaaactgt ttaatgctag agatgctgca
    15961 tatcaaaaaa taatcaaaca attatcggat ttattgcccg aagagaaaga agacacagaa
    16021 acgccatctg atgattacct atgattagta ataaatacgt tgatgaatat ataaatttgt
    16081 ggaaacaagg aaagataatt ttaaataaag aaagaattga tctctttaat tatctacaaa
    16141 cacatatata ttcacgagat gatgtatatt ttgatgaaca gaaaatcgag gattgtatca
    16201 aatttattga aaaatggtat tttccaacat taccatttca aaggtttatc atagctaata
    16261 tatttcttat agataaaaat acagatgaag ctttctttac agaatttgct attttcatgg
    16321 gacgtggagg cgggaaaaac ggtctaataa gtgctattag tgattttctt tctacgccct
    16381 tacacggagt taaagaatat cacatctcca ttgttgctaa tagtgaagat caagcaaaaa
    16441 catcgtttga tgaaatcaga accgttttaa tggataacaa acgaaataag acgggtaaaa
    16501 cgccaaaagc tccttatgaa gttagtaaag caaaaataat aaaccgtgca actaaatcgg
    16561 ttattcgata taacacatca aacacaaaaa ccaaagacgg tggacgtgag gggtgtgtta
    16621 tttttgatga aattcattat ttctttggtc ctgaaatggt aaacgtcaaa cgtggtggat
    16681 taggtaaaaa gaaaaataga agaacgtttt atataagtac tgatggtttt gttagagagg
    16741 gttatatcga tgcaatgaag cacaaaattg caagtgtatt aagtggcaag gttaaaaata
    16801 gtagattgtt tgctttttat tgtaagttag acgatccaaa agaagttgat gacagacaga
    16861 cgtgggaaaa ggcgaaccca atgttacata aaccgttatc agaatacgct aaaacactgc
    16921 taagcacgat tgaagaagaa tataacgatt taccattcaa ccgttcaaat aagcccgaat
    16981 tcatgactaa gcgaatgaat ttgcctgaag ttgaccttga aaaagtaata gcaccatgga
    17041 aagaaatact agcgactaat agagagatac caaatttaga taatcaaatg tgtattggtg
    17101 gtttagactt tgcaaacatt cgagattttg caagtgtagg gctattattc cgaaaaaacg
    17161 atgattacat ttggttagga cattcgtttg taagacaagg gtttttggat gatgtcaaat
    17221 tagaacctcc tattaaagaa tgggaaaaaa tgggattatt gaccattgtc gatgatgatg
    17281 tcattgaaat tgaatatata gttgattggt ttttaaaggc tagagaaaaa tatgggcttg
    17341 aaaaagtcat agctgataat tatagaactg atattgtaag acgtgcgttt gaggatgctg
    17401 gcataaaact tgaagtactt agaaatccaa aagcaataca tggattactt gcaccacgta
    17461 tcgatacaat gtttgcgaaa cataacgtaa tatatggaga caatcctttg atgcgttggt
    17521 ttactaataa tgttgctgta aaaatcaagc cggatggaaa taaagagtat atcaaaaaag
    17581 atgaagtcag acgtaaaacg gatggattta tggcatttgt tcatgcgtta tatagagcag
    17641 acgatatagt agacaaagac atgtctaaag cgcttgatgc attaatgagt atagatttct
    17701 aatagaggag gtgagacatg agtattctag aaaagatatt taaaactagg aaagatatat
    17761 catatatgct tgatttagat atgatagaag atctatcaca acaagcgtat gtgaaacgtt
    17821 tagcgattga tagttgtatt gaatttgttg cgcgtgctgt cgctcaaagt cattttaaag
    17881 tattggaagg taatagaatt caaaagaatg atgtttacta caagttaaat ataaaaccaa
    17941 atactgactt atcaagcgat agtttttggc aacaagttat atataaacta atttacgata
    18001 acgaggtttt aatcgtagta agtgacagca aagaattact tatcgcagat agcttttaca
    18061 gagaagagta cgctttgtat gatgatatat tcaaagatgt aacggttaaa gattatactt
    18121 atcaacgtac tttcacaatg caagaggtca tatatttaaa gtacaacaac aataaagtga
    18181 cacactttgt agaaagtcta ttcgaagatt acgggaaaat attcggaaga atgataggtg
    18241 cacaattaaa aaactatcaa ataagaggga ttttgaaatc tgcctctagc gcatatgacg
    18301 aaaagaatat agaaaaatta caagcgttca caaataaatt attcaatact tttaataaaa
    18361 atcaactagc aatcgcgcct ttgatagaag gttttgatta tgaggaatta tctaatggtg
    18421 gtaagaatag taacatgcct ttttctgaat tgagtgagct aatgagagat gcaataaaaa
    18481 atgttgcgtt gatgattggt atacctccag gtttgattta cggagaaaca gctgatttgg
    18541 aaaaaaacac gcttgtattt gagaagttct gtttaacacc tttattaaaa aagattcaga
    18601 acgaattaaa cgcgaaactc ataactcaaa gcatgtattt gaaagataca agaatagaaa
    18661 ttgtcggtgt gaataaaaaa gacccacttc aatatgctga agcaattgac aaacttgtaa
    18721 gttctggttc atttacaagg aatgaggtgc ggattatgtt aggtgaagaa ccatcagaca
    18781 atcctgaatt agacgaatac ctgattacta aaaactacga aaaagctaac agtggtgaaa
    18841 atgatgaaaa agaaaaagat gaaaacactt tgaaaggtgg tgatgaagat gaaagtggag
    18901 attaaaggcg tcatcgtttc caacgaagat aaatgggttt acgaaatgct tggtatggat
    18961 tcgacttgtc ctaaagatgt tttaacacaa ctagaattta gtgatgaaga tgttgatatt
    19021 ataattaact caaatggtgg taacctagta gctggtagtg aaatatatac acatttaaga
    19081 gctcataaag gcaaagtgaa tgttcgtatc acagcaatag cagcaagtgc ggcatcgctt
    19141 atcgcaatgg ctggtgacca catcgaaatg agtccggttg ctagaatgat gattcacaat
    19201 ccttcaagta ttgcgcaagg agaagcgaaa gatctaaatc atgctgcaga aacattagaa
    19261 catgttggtc aaataatggc tgaggcatat gcggttagag ctggtaaaaa caaacaagaa
    19321 cttgtagaaa tgatggctag ggaaacgtgg ctaaatgctg atgaagccat tgaacaaggt
    19381 tttgcggata gtaaaatgtt tgaaaacgac aatatgcaaa ttgtagcaag cgatacacaa
    19441 gtgttatcga aagatgtatt aaatcgtgta acagctttgg taagtaaaac gccagaggtt
    19501 aacattgata ttgacgcaat agcaaataaa gtaattgaaa aaataaatat gaaagaaaag
    19561 gaatcagaaa tcgatgttgc agatagtaaa gtatcagcaa atggattttc aagattcctt
    19621 ttttaataca aaaataggag gtcataaaat gactataaat ttatcggaaa cattcgcaaa
    19681 tgcgaaaaac gaatttatta atgcagtaaa caacggtgaa ccgcaagaaa gacaaaatga
    19741 attgtacggt gacatgatta accaactatt tgaagaaact aaattacaag caaaagcaga
    19801 agctgaaaga gtttctagtt tacctaaatc agcacaatct ttgagtgcaa accaaagaag
    19861 tttcttcatg gatatcaata aaaacgttaa ctataaagaa gaaaaacttt tgccagaaga
    19921 aacaattgat agaatttttg aagatttgac gacgaatcat ccgttattag ctgatttagg
    19981 tattaaaaac gctggtttgc gtttgaagtt cttaaaatcc gaaacttctg gcgtggctgt
    20041 ttggggtaaa atctatggtg aaattaaagg tcaattagat gctgcgttca gtgaagaaac
    20101 agcaattcaa aataaattga cagcgtttgt tgttttacca aaagatttaa atgattttgg
    20161 tcctgcgtgg attgaaagat ttgttcgtgt tcaaatcgaa gaagcatttg cagtggcgct
    20221 tgaaactgcg ttcttaaaag gtactggtaa agaccaacca atcggcttaa accgtcaagt
    20281 acaaaaaggt gtatcggtaa ctgagggtgc ttatccagag aaagaagaac aaggtacgct
    20341 tacatttgct aatccgcgcg ctacggttaa tgaattgacg caagtgttta aataccactc
    20401 aactaacgag aaaggtaaat cagtagcggt taaaggtaat gtaacaatgg ttgttaatcc
    20461 gtccgatgct tttgaggttc aagcacagta tacacattta aatgcaaatg gcgtatatgt
    20521 tactgcttta ccatttaatt tgaatgttat tgagtctaca gttcaagaag caggtaaggt
    20581 tttaacgtac gttaaaggtc tatatgatgg ttatttagct ggtggtatta atgttcagaa
    20641 atttaaagaa acacttgcgt tagatgatat ggatttatac actgcaaaac aatttgctta
    20701 cggcaaagcg aaagataata aagttgctgc tgtttggaaa ttagatttaa aaggacataa
    20761 accagcttta gaagataccg aagaaacact ataaaatttt atgaggtgat aaaatggtga
    20821 aatttaaagt tgttagagaa tttaaagaca tagagcacaa tcaacacaag tacaaagtag
    20881 gggagttgta tccagctgaa gggtataaca atcctcgtgt tgaattgttg acaaatcaaa
    20941 tcaaaaataa gtacgacaaa gtttatatcg tacctttaga taagctgaca aaacaagaat
    21001 tattagaact atgcgaatca ttacaaaaaa aagcgtctag ttcaatggtt aaaagtgaaa
    21061 tcatcgactt attgaatggt gaagacaatg acgattgatg atttgcttgt caaatttaaa
    21121 tcacttgaaa agattgacca taattcagag gatgagtact taaagcagtt gttaaaaatg
    21181 tcgtacgagc gtataaaaaa tcagtgcgga gtttttgaat tagagaattt aataggtcaa
    21241 gaattgatac ttatacgcgc tagatatgct tatcaagatt tattagaaca cttcaacgac
    21301 aattacagac ctgaaataat agatttttcg ttatctctaa tggaggtatc agaagatgaa
    21361 gaaagtgttt aagaaaccta gaattacaac taaacgttta aatacgcgtg ttcattttta
    21421 taagtatact gaaaataatg gtccagaagc tggagaaaaa gaagaaaaat tattatatag
    21481 ctgttgggcg agtattgatg gtgtctggtt acgtgaatta gaacaagcta tctcaaacgg
    21541 aacgcaaaat gacattaaat tgtatattcg tgatccgcaa ggtgattatt tacccagtga
    21601 agaacattat cttgaaattg aatcaagata tttcaaaaat cgtttgaata taaagcaagt
    21661 atcaccagat ttggataata aagactttat tatgattcgt ggaggatata gttcatgagt
    21721 gtgaaagtga caggtgataa agcattagaa agagaattag aaaaacattt tggcataaaa
    21781 gagatggtaa aagttcaaga taaggcgtta atagctggtg ctaaggtaat tgttgaagaa
    21841 ataaaaaaac aactcaaacc ttcagaagac tcaggagcac tgattagtga gattggtcgt
    21901 actgaacctg aatggataaa ggggaaacgt actgttacaa ttaggtggcg tgggcctttt
    21961 gaacgattta gaatagtaca tttaattgaa aatggtcatg ttgagaaaaa gtcaggaaaa
    22021 tttgtaaaac ctaaagctat gggtgggatt aatagagcaa taagacaagg gcaaaataag
    22081 tattttgaga cgctaaaaag ggagttgaaa aaattgtgat tgatattttg tacaaagttc
    22141 atgaagtgat tagtcaagac agaattatta gagagcacgt aaatatcaat aatattaagt
    22201 tcaataaata ccctaatgta aaagatactg atgtaccttt tattgttatt gacgatatcg
    22261 acgacccaat acctacaact tatactgacg gagatgagtg tgcatatagt tatattgtcc
    22321 aaatagatgt ttttgttaag tacaatgatg aatataatgc gagaatcata agaaataaga
    22381 tatctaatcg cattcaaaag ttattatggt ctgaactaaa aatgggaaat gtttcaaatg
    22441 gaaaaccgga atatatagaa gaatttaaaa catatagaag ctctcgcgtt tacgagggca
    22501 ttttttataa ggaggaaaat taaatggcag taaaacatgc aagtgcgcca aaggcgtata
    22561 ttaacattac tggtttaggt ttcgctaaat taacgaaaga aggcgcggaa ttaaaatata
    22621 gtgatattac aaaaacaaga ggattacaaa aaattggtgt tgaaactggt ggagaactaa
    22681 aaacagctta tgctgatggc ggtccaattg aatcagggaa tacagacgga gaaggtaaaa
    22741 tctcattaca aatgcatgcg ttccctaaag agattcgcaa aattgttttt aatgaagatt
    22801 atgatgaaga tggcgtttac gaagagaaac aaggtaaaca aaacaattac gtagctgtat
    22861 ggttcagaca agagcgtaga gacggtacat ttagaacagt tttattacct aaagttatgt
    22921 ttacaaatcc taaaatcgat ggagaaacgg ctgagaaaga ttgggatttc tcaagtgaag
    22981 aggttgaagg tgaggcactt ttccctttag ttgataataa aaagtctgta cgtaaatata
    23041 tctttgactc agctaacatg acaaatcatg gtggcgacgg tgaaaaaggc gaagaggctt
    23101 tcttaaagaa aattttaggc gaagaatata ctggaaacgt gacagaggat aacgaagaaa
    23161 ctttgtaacg aaaccggctt catcggaaac tgcggtaaag tcggttaata taccagatag
    23221 cattaaaaca cttaaagttg gcgacacata cgatttaaat attgtagtag agccatctaa
    23281 tcaaagtaag ttattgaaat acacaacaga tcaaacgaat attgtatcaa tcaatagaga
    23341 tggtcaagtt actgcggaag cacaaggcat tgctacggtt aaagcaacag ttggtaatat
    23401 gagtgacact ataacaataa atgtagaagc ataagagggg gcaacccctc tattttattt
    23461 gaaaataagg agagtattat aaaatggcaa aattaaaacg taacattatt caattagtag
    23521 aagacccgaa agcaaatgaa attaaattac aaacgtactt aacaccacac ttcatttcat
    23581 ttgaaattgt atacgaagca atggatttaa tcgatgatat tgaggacgaa aatagcacga
    23641 tgaaaccaag agaaatcgct gacagattga tggatatggt tgtaaaaatt tacgataacc
    23701 aattcacagt taaagaccta aaagaacgta tgcatgcacc tgatggaatg aatgcacttc
    23761 gtgaacaagt gattttcatt actcaaggtc agcaaactga ggaaactaga aattttatcc
    23821 agaacatgaa ataaagcctg aagatttaac atataaagca atgttgaaaa atatggatac
    23881 tctcatgatg gacttaattg aaaatggtaa agacgctaac gaagttttaa aaatgccatt
    23941 tcattatgta ctttccatat atcaaaataa aaacaatgac atttctgaag aaaaagcaga
    24001 ggctttaatt gatgcgtttt aaccttaacc gtttggttag ggttattttt ttgaactttt
    24061 ttagaaagga ggtaaaaaat gggagaaaga ataaaaggtt tatctatagg tttggattta
    24121 gatgcagcaa atttaaatag atcatttgca gaaatcaaac gaaactttaa aactttaaat
    24181 tctgacttaa agttaaccgg taacaacttc aaatataccg aaaaatcaac tcatagttac
    24241 aaacaaagga ttaaagaact tgatggaact atcacaggtt ataagaaaaa cgttgatgat
    24301 ttagccaagc aatatggcaa ggtatctcaa gaacagggcg aaaacagcgc ggaagctcaa
    24361 aaattacgac aagaatataa caaacaagca aatgagctga attttttaga aaaagaacta
    24421 gaaaaaacaa caactgagtt tgaagagttc aaaaaagctc aagttgaagc tcaaagaatg
    24481 gcagaaagtg gctggggaaa aaccagtaaa gtttttgaaa gtatgggacc taaattaaca
    24541 aaaatgggtg atggtttaaa atccattggt aaaggtttga tgattggtgt aactgcacct
    24601 gttttaggta ttgcagcagc atcaggaaaa gcttttgcag aagttgataa aggtttagat
    24661 acagttaccc aagcaacagg agcaaccggc ggagagctta agaagttgca gaattcattt
    24721 aaagatgttt atggcaactt tccagcagac gctgagactg taggcggtgt tttaggggaa
    24781 gttaacacaa ggttaggttt cactggcaaa gaacttgaga gtgccacaga gtcattcttg
    24841 aaatttagtc acataacagg ttctgacggc gtacaagccg ttcaattaat tacgcgtgca
    24901 atgggtgatg caggtattga agctgatgag tatcaaagta tacttgatat ggtagcgaaa
    24961 gcagcacagg ctagcggtat aagtgttgat acattagctg atagtattac taaatacggt
    25021 gctccaatga gggctatggg ctttgagatg aaagaatcaa tcgctttatt ctctcaattg
    25081 gagaaatcag gtgttaatac tgaaatagcc ttcagtggtt tgaaaaaagc tatatccaat
    25141 tggggtaaag cgggtaaaga cccaagagaa gaatttaaga agacattagc agaaattgaa
    25201 aggacaccgg atatagctag cgcaacaagt ttagcgattg aagcatttgg tgcaaaagca
    25261 ggtcctgatt tagcagatgc tattaaaggc ggtcgcttta gttaccaaga gttcttaaaa
    25321 actatcgaag attcgcaagg aacggtcaat cagacattta aagattctga aagtggctcc
    25381 gaaagattta aagtagcaat gaataaactt aaattagtag gtgctgatgt atgggcttct
    25441 attgaaagtg cgtttgctcc agtcatggaa gaattaatca aaaagctatc tgtagcagtt
    25501 gattggtttt caagtttaag tgatggatct aaaaggtcga ttgttatatt tggtggtatt
    25561 gctgctgcaa tcggtcctgt agtttttgga ttaggtgcat ttataagtac aattggcaat
    25621 tcagtaactg tattagcccc attgctagct agtattacaa aggctggtgg attgattagt
    25681 tttttatcga ctaaagtacc tatattagga actgtcttca cagctttaac tggtccaatt
    25741 ggcattgtat taggtgtatt ggctggttta gcagtcgcat ttacaattgc ttataagaaa
    25801 tctgaaacat ttagaaattt tgttaatggt gcaattgaaa gtgttaaaca aacatttagt
    25861 aattttattc aatttattca acctttcatt gattctgtta aaaacatctt taaacaagcg
    25921 atatcagcaa tagttgattt tgctaaagat atttggagtc aaatcaatgg attctttaat
    25981 gaaaatggaa tttccattgt tcaagcactt caaaatatat gcaactttat taaagcgata
    26041 tttgaattta ttttaaattt tgtaattaaa ccaattatgt tcgcaatctg gcaagtgatg
    26101 caatttattt ggccggcggt taaagccttg attgtcagta cttgggagaa tataaaagga
    26161 gtaatacaag gtgctttaaa tataatactt ggctttatta agttcttttc aagtttattc
    26221 actggtaatt ggcgaggtgt ttgggacggt attgtgatga tactaaaagg cactgtgcag
    26281 ttaatttgga atttaataca actgtggttt gtaggtaaga ttctaggtgt tgttagatac
    26341 tttggtggat tgcttaaagg tttaatatcc ggtatctggg gtgttatcaa aggtattttc
    26401 acaaaatcat tatctgcaat ttggaatgca acgaaaagta tttttggttt cttatacaat
    26461 agtgttaaat ctattttcac taatatgaaa aactggttat ctagtacgtg gaataatatc
    26521 aaaagcaata ccgtcggcaa ggctcattcg ttatttacgg gtgtaaggtc taaattcaca
    26581 agtttatgga atgcgacgaa agatatattt actaaattaa gaaattggat gtcaaacatc
    26641 tggaactcta ttaaagataa cactgtaggt atagctggtc gcttatggga taaagtacgt
    26701 aatatcttcg gaaacatgcg tgacggttta aaatctatca ttggtaaaat taaagatcat
    26761 atcggcggta tggtagatgc tattaaaaaa ggacttaata aattaattga aggtttaaac
    26821 tgggtcggtg gtaagctggg aatggataaa atacctaagt tacacactgg tacagagcac
    26881 acacatacta ctacaagatt agttaagaat gggaaaattg cacatgatac attcgctaca
    26941 gttggagaca aaggacgcgg taatggtcct aatggtttca ggaatgaaat gattgagtat
    27001 cctaacggta aacgtgtaat cacacctaat acagatacta ccgcttattt acctaaaggc
    27061 tcaaaagtat acaacggtgc acaaacttat tcaatgttaa acggaacgct tccaagattt
    27121 agtttaggta ctatgtggaa agatattaaa tctggtgcat catcggcatt taactggaca
    27181 aaagataaaa taggtaaagg taccaaatgg cttggcgata aagttggcga tgttttagat
    27241 tttatggaaa atccaggcaa acttttaaat tatatacttg aagcttttgg aattgatttc
    27301 aattctttaa ctaaaggtat gggaattgca ggcgacataa caaaagctgc atggtctaag
    27361 attaagaaaa gtgctactga ttggataaaa gaaaatttag aagctatggg cggtggcgat
    27421 ttagtcggcg gaatattaga ccctgacaaa attaattatc attatggacg taccgcagct
    27481 tataccgctg caactggaag accatttcat gaaggtgtcg attttccatt tgtatatcaa
    27541 gaagttagaa cgccgatggg tggcagactt acaagaatgc catttatgtc tggtggttat
    27601 ggtaattatg taaaaattac tagtggagtt atcgatatgc tatttgcgca tttgaaaaac
    27661 tttagcaaat caccacctag tggcacgatg gtaaagcccg gtgatgttgt tggtttaact
    27721 ggtaataccg gatttagtac aggaccacat ttacattttg aaatgaggag aaatggacga
    27781 cattttgacc ctgaaccata tttaaggaat gctaagaaaa aaggaagatt atcaataggt
    27841 ggtggcggtg ctacttctgg aagtggcgca acttatgcca gtcgagtaat ccgacaagcg
    27901 caaagtattt taggtggtcg ttataaaggt aaatggattc atgaccaaat gatgcgcgtt
    27961 gcaaaacgtg aaagtaacta ccagtcaaat gcagtgaata actgggatat aaatgctcaa
    28021 agaggagacc catcaagagg attattccaa atcatcggct caacttttag agcaaacgct
    28081 aaacgtggat atactaactt taataatccg gttcatcaag gtatttcagc aatgcagtac
    28141 attgttagac gctacggttg ggatggattt aagcgtgcgg gtgattacgc atatgctaca
    28201 ggcggaaaag tttttgatgg ttggtataac ttaggtgaag acggtcatcc agaatgggtt
    28261 attccaacag atccagctcg tagaaatgat gcaatgaaga tgttacatta tgcagctgcg
    28321 gaagttagag ggaaaaacgc aagtaagaat aaacgaccta gtcaattgtc tagtgtaaat
    28381 gggtttgatg acccaagctt attattgaaa atgattgaac aacagcaaca acaaatagct
    28441 ttattactga agatagcgca atctaacgat gtgattgcag ataaagatta tcagccgatt
    28501 attgacgaat acgcttttga taaaaaggtg aacgcgtcta tagaaaagcg agaaaggcaa
    28561 gaatcaacaa aagtaaagtt tagaaaagga ggaattgcta ttcaatgata gacactatta
    28621 aagtgaataa caaaacaatt ccttggttgt atgtcgaaag agggtttgaa ataccctctt
    28681 ttaattatgt tttaaaaaca gaaaatgtag atggacgttc ggggtctata tataaagggc
    28741 gtaggcttga atcttatagt tttgatatac ctttggtggt acgtaatgac tatttatctc
    28801 acaacggcat taaaacacat gatgacgtct tgaatgaatt agtaaagttt tttaactacg
    28861 aggaacaagt taaattacaa ttcaaatcta aagattggta ctggaacgct tattttgaag
    28921 gaccaataaa gctgcacaaa gaatttgcaa tacctgttaa gttcactatc aaagtagtac
    28981 taacagaccc ttacaaatat tcagtaacag gaaataaaaa tactgcgatt tcagaccaag
    29041 tatcggttgt taataacggt actgctgaca cacctttaat cgttgaagcc cgagcaataa
    29101 aaccatctag ttattttatg attaccaaaa acgacgaaga ttatttcatg gttggcgacg
    29161 atgaagtaac caaagaagtt aaggattaca tgccccctgt ttaccatact gaatttcgtg
    29221 atttcaaagg gtggaataaa atgactgctg aagatattcc aagtaatgac ttaggtggta
    29281 aggtcggcgg tgactttgtg atatccaatc ttggcgaagg atataaagca actaattttc
    29341 ctgatgcaaa aggttgggtt ggtgctggca cgaaacgagg gctccctaaa gcgatgacag
    29401 attttcaaat tacctataaa tgtattgttg aacaaaaagg taaaggtgcc ggaagaacag
    29461 cacaacatat ttatgatagt gatggtaagt tacttgcttc tattggttat gaaaataaat
    29521 atcatgatag aaaaataggc catattgttg ttacgttgta taaccaaaaa ggagacccca
    29581 aaaaggtata cgactatcag aataaaccga taatgtataa cttggacaga atcgttgttt
    29641 atatgcggct cagaagagta ggtaataaat tttctattaa aacttggaaa tttgatcaca
    29701 ttaaagaccc agatagacgt aaacctattg atatggatga gaaagagtgg atagatggcg
    29761 gtaagtttta tcagcgtcca gcttctatca tagctatcta tagtgcgaag tataacggtt
    29821 ataagtggat ggagatgaat ggattaggtt cattcaatac ggagattcta ccgaaaccga
    29881 aaggcgcaag ggatgtcatt atacaaaaag gtgatttagt gaaaatagat atgcaagcaa
    29941 aaagtgttgt catcaatgag gaaccaatgt tgagcgagaa atcgtttgga agtaattatt
    30001 tcaatgttga ttctgggtac agtgaattaa tcatacaacc tgaaaacgtc tttgatacga
    30061 cggttaaatg gcaagataga tatttataga aaggagatga gagtgtgata catgttttag
    30121 attttaacga caagattata gatttccttt ctactgatga cccttcctta gttagagcga
    30181 ttcataaacg taatgttaat gacaattcag aaatgcttga actgctcata tcatcagaaa
    30241 gagctgaaaa gttccgtgaa cgacatcgtg ttattataag ggattcaaac aaacagtggc
    30301 gtgaatttat tattaactgg gttcaagata cgatggacgg ctacacagag atagagtgtg
    30361 tagcgtcata tctcgctgat ataacaacag ctaagccata cgcaccaggt aaacttgaga
    30421 aaaagacaac atcagaagcg ctgaaagaag tactaagtga tacgggttgg gaagtttctg
    30481 aacaaaccga atacgatggc ttacgtacta cgtcatggac ttcttatcaa actagatatg
    30541 aagttttaaa gcaattatgt acaacctata aaatggcatt ggatttttat atagagctta
    30601 gttctaatac cgtcaaaggt agatatgtgg tactcaaaaa gaaaaacagc ttattcaaag
    30661 gtaaagaaat tgagtatggt aaagatttgg ttgggttaac taggaagatt gatatgtcag
    30721 aaatcaaaac agcattaatt gctgtgggac ccgaaaatga caaaggaaag cgtttagagt
    30781 tagttgtgac tgatgacgaa gcacaaagtc aattcaactt acctacccgt tatatttggg
    30841 gaatatacga acctcaatca gatgatcaaa atatgaatga aacacggttg cgttctttag
    30901 ccaaaacaga gttaaataaa cgtaagtcgg cagttatgtc atatgagatt acttctactg
    30961 atttggaagt tacgtatccg cacgagatta tatcaattgg tgatacagtc agagtaaaac
    31021 atagagattt taacccgcca ttgtatgtag aggcagaagt tattgccgaa gaatataaca
    31081 taatttcaga aaatagcaca tatacattcg gtcaacctaa agagttcaaa gaatcagaat
    31141 tacgagaaga gtttaacaaa cgattgaaca taatacatca aaagttaaac gataatatta
    31201 gcaatatcaa cactatagtt aaagatgttg tagatggtga attagaatac tttgaacaca
    31261 aaatacacaa aagtgataca ccgccagaaa atccagtcaa tgatatgctt tggtatgata
    31321 caagtaaccc tgatgttgct gtcttgcgta gatattggaa tggtcgatgg attgaagcaa
    31381 caccaaatga tgttgaaaaa ttaggtggta taacaagaga gaaagcgcta ttcagtgaat
    31441 taaacaatat ttttattaat ttatctatac aacacgctag tcttttgtca gaagctacag
    31501 aattactgaa tagcgagtac ttagtagata atgatttgaa agcggactta caagcaagtt
    31561 tagacgctgt gattgatgtt tataatcaaa ttaaaaataa tttagaatct atgacacccg
    31621 aaactgcaac gattggtcgg ttggtagata cacaagcttt atttcttgag tatagaaaga
    31681 aattacaaga tgtttataca gatgtagaag atgtcaaaat cgccatttca gatagattta
    31741 aattattaca gtcacaatac actgatgaaa aatataaaga agcgttggaa ataatagcaa
    31801 caaaatttgg tttaacggtg aatgaagatt tgcagttagt cggagaacct aatgttgtta
    31861 aatcagctat tgaagcagct agagaatcca caaaagaaca attacgtgac tatgtaaaaa
    31921 catcggacta taaaacagac aaagacggta ttgttgaacg tttagatact gctgaagctg
    31981 agagaacgac tttaaaaggt gaaatcaaag ataaagttac gttaaacgaa tatcgaaacg
    32041 gattggaaga acaaaaacaa tatactgatg accagttaag tgatttgtcc aataatcctg
    32101 agattaaagc aagtattgaa caagcaaatc aagaagcgca agaagcttta aaatcataca
    32161 ttgatgctca agataatctt aaagagaagg aatcgcaagc gtatgctgat ggtaaaattt
    32221 cggaagaaga gcaacgcgct atacaagatg ctcaagctaa acttgaagag gcaaaacaaa
    32281 acgcagaact aaaggctaga aacgctgaaa agaaagctaa tgcttataca gacaacaagg
    32341 tcaaagaaag cacagatgca cagaggagaa cactgactcg ctatggttct caaattatac
    32401 aaaatggtaa ggaaatcaaa ttaagaacta ctaaagaaga gtttaatgca accaatcgta
    32461 cactttcaaa tatattaaac gagattgttc aaaacgttac agatggaaca acaatcagat
    32521 atgatgataa cggagtggct caagctttga atgtggggcc acgtggtatt agattaaatg
    32581 ctgataaaat tgatattaac ggtaatagag aaataaacct tcttatccaa aatatgcgag
    32641 ataaagtaga taaaaccgat attgtcaaca gccttaattt atcaagagag ggtcttgata
    32701 tcaatgttaa tagaattgga attaaaggcg gtgacaataa cagatatgtt caaatacaga
    32761 atgattctat tgaactaggt ggtattgtgc aacgtacttg gagagggaaa cgttcaacag
    32821 acgatatttt tacgcgactg aaagacggtc acctaagatt tagaaataac accgctggcg
    32881 gttcacttta tatgtcacat tttggtattt cgacttatat tgatggtgaa ggtgaagacg
    32941 gtggttcatc tggtacgatt caatggtggg ataaaactta cagtgatagt ggcatgaatg
    33001 gtataacaat caattcctat ggtggtgtcg ttgcactaac gtcagataat aatcgggttg
    33061 ttctggagtc ttacgcttca tcgaatatca aaagcaaaca ggcaccggtg tatttatatc
    33121 caaacacaga caaagtgcct ggattaaacc gatttgcatt cacgctgtct aatgcagata
    33181 atgcttattc gagtgacggt tatattatgt ttggttctga tgagaactat gattacggtg
    33241 cgggtatcag gttttctaaa gaaagaaata aaggtcttgt tcaaattgtt aatggacgat
    33301 atgcaacagg tggagataca acaatcgaag cagggtatgg caaatttaat atgctgaaac
    33361 gacgtgatgg taataggtat attcatatac agagtacaga cctactgtct gtaggttcag
    33421 atgatgcagg agataggata gcttctaact caatttatag acgtacttat tcggccgcag
    33481 ctaatttgca tattacttct gctggcacaa ttgggcgttc gacatcagcg cgtaaataca
    33541 agttatctat cgaaaatcaa tataacgata gagatgaaca actggaacat tcaaaagcta
    33601 ttcttaactt acctattaga acgtggtttg ataaagctga gtctgaaatt ttagctagag
    33661 agctgagaga agatagaaaa ttatcggaag acacctataa acttgataga tacgtaggtt
    33721 tgattgctga agaggtggag aatttaggat taaaagagtt tgtcacgtat gatgacaaag
    33781 gagaaattga aggtatagcg tatgatcgtc tatggattca tcttatccct gttatcaaag
    33841 aacaacaact aagaatcaag aaattggagg agtcaaagaa tgcaggataa caaacaagga
    33901 ttacaagcta atcctgaata tacaattcat tatttatcac aggaaattat gaggttaaca
    33961 caagaaaacg cgatgttaaa agcgtatata caagaaaata aagaaaatca acaatgtgct
    34021 gaggaagagt aatccttagc actattttta tacaaaaatt taaggaggtc atttaattat
    34081 ggcaaaagaa attatcaaca atacagaaag gtttatttta gtacaaatcg acaaagaagg
    34141 tacagaacgt gtagtatatc aagatttcac aggaagtttt acaacttctg aaatggttaa
    34201 ccatgctcaa gattttaaat ctgaagaaaa cgctaagaaa attgcggaga cgttaaattt
    34261 gttatatcaa ttaactaaca aaaaacaacg tgtgaaagta gtgaaagaag ttgtggatag
    34321 aactgacttg tcatctgata aaacagttga tagcgaaaca atgtaactat actaagttat
    34381 gagtattaca ctcatagctt tcttagaaag taggtgtagt tttggatgat attcagaaaa
    34441 taaaaaaaga gctttctgaa ttagttgaac gtgtggatga tgttgaagta ctagcaaatg
    34501 aaacagctga tcacgtgctt gaacttagag aggaacataa gcaacatcat aatgaactaa
    34561 gagaatctca taaagaactt aaagacaagc aagataaagt tgtaggtgag aatttagagc
    34621 aaacaaagat attaaacaga attgaagaaa ggtatcaaac gcaagtagat gttgcgcaaa
    34681 aaaacgaaga aaagacactc gcccaaaata aatggctcgt aggtgccata tgggcgcttg
    34741 tgacaattgt tatgattgca gtcattactg catcaattac tgcgttatta ccttaaggga
    34801 ggtggacaaa atgagttggg caagatggtt gtcatgctat ttgtatggtc gtaaatgtaa
    34861 ataatatttt tggtcagtgc ttcagcactg gctttttatt ttgattgaaa agaggtacgt
    34921 acatggtatt acacagctca aaagacagga agcatactgc aagtgaagtt gggaagtgtt
    34981 gtcaatacca agtaagtaag atatctgaaa tgtataatag agtaaaaatg aaatcttttt
    35041 attatattat agacaagtat aaaaaaggta tagtaatata tgtatgtata agtaaataat
    35101 gataattcta taattattgt atataactaa taattacttt gacaaaaata atctattatc
    35161 caaatatttt agataataaa aagtttgtat ggaattatgc tttagaggtg agcaaaatga
    35221 aaaaaacagc atttatacta cttttattca ttgccctaac gtggacaaca agtccacttg
    35281 taaatggtag cgagaaaagc gaagaaataa atgaaaaaga tttgcgaaaa aagtctgaat
    35341 tgcagggaac agctttaggc aatcttaaac aaatctatta ttacaatgaa aaagctaaaa
    35401 ctgaaaataa agagagtcac gatcaatttt tacagcatac tatattgttt aaaggctttt
    35461 ttacaaatca ttcatggtat aacgatttat tagtagattt tgattcaaag gatattgttg
    35521 ataaatataa agggaaaaaa gtagacttat atggtgctta ttatggttat caatgtgcgg
    35581 gtggtacacc aaacaaaaca gcttgcatgt atggtggtgt aacgttacat gataataatc
    35641 gattgaccga agagaaaaaa gtgccaatca atttatggct agacggtaaa caaaatacag
    35701 tacctttgga aacggttaaa acgaataaga aaaatgtaac tgttcaggag ttggatcttc
    35761 aagcaagacg ttatttacag gaaaaatata atttatataa ctctgatgtt tttgatggga
    35821 aggttcagag gggattaatc gtgtttcata cttctacaga accttcggtt aattacgatt
    35881 tatttggtgc tcaaggacag aattcaaata cactattaag aatatataga gataataaaa
    35941 cgattaactc tgaaaacatg catattgata tatatttata tacaagttaa acatggtagt
    36001 tttgaacacg taatgttcag attattatga accgagaata atctgaaagt ttacaagcag
    36061 taaaaaaagt atatgtgcta taatatgctt tgagcaagtt ggatagatgg tggctatctg
    36121 agtataagga ggtggtgcct atggtggcat tacataaatc tttagaaagg agacgcctaa
    36181 tgattacaat tagtaccatg ttgcagtttg gtttattcct tattgcattg ataggtctag
    36241 taatcaagct tattgaatta agcaataaaa aataaccatc gctaactttg gctggtttcg
    36301 atggttaaat ggttattaat ttaatcttta atctaaaata gccaccgtct ttttaacggg
    36361 ctcattaggg taacatgttt gcgcatgttg ccctttttct atatataaat taacacacca
    36421 taatataaat atcaaataga cggcttatta gtcgtctttt tattttggat aaaaggagat
    36481 aagaatatga ttaattggaa aattagaatg aaacaaaaat cattttgggt agcgatattg
    36541 tcagctatct ttttatttgc tcaaaacatc gccaaagcta ttgggtatga tatccaagtt
    36601 tatacagagc aattaacaga cggtttaaac gctatattag gatttttagt attaactggt
    36661 gtgattcaag acccgactac taaaggtata ggtgatagcc accaagcttt agaatatgaa
    36721 gaaccaagaa gaaaatacta ggaggtaaaa taatgaaaac atacagtgaa gcaagagcaa
    36781 ggttacgttg gtatcaaggt agatatattg attttgacgg ttggtatggt taccaatgtg
    36841 cagatttagc agttgattac atttattggt tgttagaaat tagaatgtgg ggaaatgcaa
    36901 aagatgcaat caataacgat tttaaaaaca tggcaacagt atatgaaaac acaccatcgt
    36961 ttgttccaca aataggtgat gtggctgtat ttaccaaagg aatatataaa caatacggtc
    37021 atattggttt agtgtttaat ggtggtaata caaatcaatt tttaattttg gaacagaact
    37081 atgacggtaa cgcaaatacg cctgcaaagt tacgttggga taattattac ggctgtactc
    37141 actttattag acctaagtat aaaagtgagg gcttaatgaa taagatcaca aataaagtta
    37201 aaccacctgc tcaaaaagca gtcggtaaat ctgcaagtaa aataacagtt ggaagtaaag
    37261 cgccttataa ccttaaatgg tcaaaaggtg cttattttaa tgcgaaaatc gacggcttag
    37321 gtgctacttc agccactaga tacggtgata atcgtactaa ctatagattc gatgttggac
    37381 aggctgtata cgcgcctgga acattaatat atgtgtttga aattatagat ggttggtgtc
    37441 gcatttattg gaacaatcat aatgagtgga tatggcatga gagattgatt gtgaaagaag
    37501 tgttttaatt cttaggttaa aatgttaaat atttgttaat tattttttaa tgtaagttta
    37561 gtttctttta atattttatt gatttttaat attttctcaa tataaaatga agttgttgat
    37621 atttatcatc ttaaataagg gtgttagcta taaaaagaga taaataaaaa caaatatatt
    37681 atatttggag gaagcgccat gctcaaaaga agtttattat ttttaactgt tttattgtta
    37741 ttattctcat tttcttcaat tactaatgag gtaagtgcat caagttcatt cgacaaagga
    37801 aaatataaaa aaggcgatga cgcgagttat tttgaaccaa caggcccgta tttgatggta
    37861 aatgtgactg gagttgatgg taaaggaaat gaattgctat cccctcgtta tgtcgagttt
    37921 cctattaaac ctgggactac acttacaaaa gaaaaaattg aatactatgt cgaatgggca
    37981 ttagatgcga cagcatataa agagtttaga gtagttgaat tagatccaag cgcaaagatc
    38041 gaagtcactt attatgataa gaataagaaa aaagaagaaa cgaagtcttt ccctataaca
    38101 gaaaaaggtt ttgtagtccc agatttatca gagcatatta aaaaccctgg attcaactta
    38161 attacaaagg ttgttataga aaagaaataa aacaaaatag ttgtttattg tagaaagtaa
    38221 tgtcttgatt gaatatgtgt agtgagaatt atctttcatc aaattctcat tcatgcacga
    38281 atggttctgc cctgcctaat cagatattag gtgacttatg gggagaaatc agttaggatg
    38341 aaaaagtgga tgatcctttt ctaggcaggt acttcggtac ttgcctattt ttttatgtta
    38401 tagctagcct tcgggctagt tttttgttat gatgtgttac acatgcatca actatttaca
    38461 tctatccttg ttcacccaag catgtcactg ggtgtttttt tcttatgata gagagcatag
    38521 ttttcatact actcccccgt agtatatatg actttagcat tcccgtataa tagtttacgg
    38581 ggtgcttttt atgttataat taattgtata tagtaggagt gaactatata gcctgttaag
    38641 tggcctagta acctaacact tatcctggca attgacaccc tttttgccct tcactcgata
    38701 catatatctc aacaacatag aaatattaca gtcgctacac cgcatcttaa atggtgtggt
    38761 tatttttatt ggaagtgtgt atcaggtatc agtaatgtta aaacaccagc taaaaatgaa
    38821 aagaatccac cagtgccagc aggttataca ctcgataaaa ataataatgt accgtataaa
    38881 aaagagattg gttattacac agttgccaat gttaaaggta ataacgtgag ggatggctat
    38941 tcaactaatt caagaattac aggtgtatta cccaataacg caacgatcaa atatgacggc
    39001 gcatattgca tcaatggcta tagatggatt acttatattg ctaatagtgg acaacgtcgt
    39061 tatatagcga caggagaggt agacaaggca ggtaatagaa taagcggttt tggtaagttt
    39121 agtgcagttt gaaaaaagtg tgtaaaattt catatgaagt taattaattt attatagaat
    39181 agtttaaaat tatgctataa tcattttaga cacagcaatg tgttcaaatt ttcatctatt
    39241 cataagctag ccttcgggct agtttttttg tgctatatat ttgttttaat taaataaaat
    39301 tagataatgc aatagtagcc attttatgtt aatattacct tgggcgtttt caaggagcgc
    39361 ctttcatttt ttatgtattg ctccccttcg gggctagtat attaaattta tttttgcgct
    39421 ttccaaatca atgtatatgt gttatattgt ttatgggaag taggtaagca tttcggtgct
    39481 tacctttttt tgtttttcta taaatacaat aaggtatgtc aatttgataa tttattaatt
    39541 ttcatttaat aagaagatct atatagttaa tgaataatta atgtactttt ttttagttag
    39601 tcattaaaat aaattagtac taattactaa ggagaataaa aaatgaaaat tagaaaatct
    39661 atacttgcgg gaactttagc aatcgtttta gcatcaccac tagtaactaa tctagataaa
    39721 aatgaggcac aagctagcac aagcttgcca acatcgaatg aatatcaaaa cgaaaagtta
    39781 gctaatgaat taaaatcgtt attagatgaa ctaaatgtta atgaattagc tactggaagt
    39841 ttaaacactt attataagcg aactataaaa atttcaggtc taaaagcaat gtatgctctt
    39901 aagtcaaaag actttaagaa aatgtcagaa gcaaaatatc aacttcaaaa gatttataac
    39961 gaaattgacg aagcactaaa aagtaaatat taaaaaaacc acccttttac gggtggtttt
    40021 aattttctag ataatataaa agtgttcata aataaaacag tataggcaaa caataaagta
    40081 ttgaaaaaag taagtttaat atgaaaattg ttaaatgaac gacatctttt gtttttataa
    40141 atatcaagaa aataatcaaa ctcaaaataa ataacgtaac tgtagtcata ggcgtccata
    40201 cataatcagc attagtcatt aagaatggtg cagccattat gaaaaaattt ataatgcaga
    40261 tgaaatagac aattagacta taaattagat aaataacaat acacaccctt cataaataaa
    40321 taatttaaat cctatatatt ttaacaaaag taacacacag aagtgtagaa aataaaaaat
    40381 attggtaaat aaaatcaata agtttaacca atatgttgct cgcttcatac cgtatattgc
    40441 aacaaaaatt ccgattaaga aaaatatagc ccctatgata aaacagaaat ccgatgctga
    40501 attattaaaa aatgaggtgt ttagagttag aaaatgagtt aatgagttga ctataactaa
    40561 taagatatta attatatttg tatggttctt cacatgatac ctccaagtaa aaaaatctaa
    40621 ttaataaagt gaa
//
LOCUS       DATTQF010000009        40487 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.009, whole genome shotgun sequence.
ACCESSION   DATTQF010000009 DATTQF010000000
VERSION     DATTQF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.009"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            237..32078
                     /gene="ebh"
                     /locus_tag="VB241_000396"
     CDS             237..32078
                     /gene="ebh"
                     /locus_tag="VB241_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HEP1243728.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGLNTSQAQAAETNQ
                     PASALKQKQQNGDTQTENREVEVQNSQNGQSLSAPIENEQPNNNQTNHVDASAVQSST
                     TEHDQPVSQNEQAKKDTAAATPTQSAKAASKHEQSESRAANKKENDNKATHVESHEAN
                     VVTASDSSDSGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKINTLL
                     GSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSSRIQTRSIESRAAEPRSVSD
                     YQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDAKEIALITAK
                     QTGDGYQWVIKFNKGHAPHENMIYWFALPAGQTPVGRTEFVTVNADGTNVQWSNGAGA
                     GANKPLPEMWPYGVNDSRSWDYKIRNRSGQVIYDWPTVHINSLKDLARASDYFSEAGA
                     TPATKAFGRQTFEYINGERPTESPGVPKVYTFIGKGDASYTISFKTQGPTIDKLYYAA
                     GGRALEYNQLFMYSQLYVESTQDYQQRLNGLRQVVNRTYRIGTTKRVEVSQGNVQTKK
                     VLESTNLNIDDFMDDPLSYVKTPSNKVLGFYPTSANTNAFRPGGVNPLNEYQLSQLFT
                     DDKLQQAARTGSPIRLMIGFDYPDAFGNGETLVPVNLTVLPEIQHNIKFFKNDDGQNI
                     ADKPASKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQITGI
                     PRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTPRITT
                     TAEELRGTALQKVPVTVTDIPLDPSALVYLVIPTSQTRDGGSEADQIPSGYTKIATGT
                     PDGVHSTITIRPEDYVVFIPPVGNQIRALIFYNNVVASNMSNAVTILPDDIPPTINNP
                     VGLNAKYYRGDEVSFTMGVSDRHSGLKSTTITTLPSGWTSNLTKSDKKNGSLAISGRV
                     SMNQAYNSDITFKVSATDNVNNTTNDSQSKHVTVHVGKISDDAHPIVLGNSEKVVVVN
                     PTALTGDEKQRITTAFMNKNQNIRGYLASSNPVTVDNHGNVTLQYRDGSSTTLDATNV
                     MTYEPVVKPEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPSSSFTNITSD
                     RTIPTAQEVSQMNAGTQLYHIVATNAYHKDTEDFYITLKIIDVKQPEGDQRVYRMSTY
                     DITTDEISKVKQAFINANRDAISFAEGDISVTNTPNGSNVSTITVNINKGRLTKSFTS
                     NLNNMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGTQITT
                     NDILTLLKATTTVPGLRNNIAGNEKAQAEAGGRPNYKTTGYSQSNPTSDGQRQFTLNG
                     QVIQIMDIINPSNGFGGQPVTNSNVRANHSNSTVVSVNESAANGAGAFTIDHVVKNNS
                     THNAADAVYKAQLYLSPYGPKQYVEHLNQNTDNTNEAINIYFVPSDLVNPTISVGNYT
                     NHQVFSGETFTNTITANDNFGVQSVTVPTTSQLTGTVDNNHQHVSATAPNVTSTTNKT
                     INLVATDTSGNTATTSFNVTIKPLRDKYRVGTSSTAANPVRIANISNNATVSQADQTA
                     IINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNAQVTVTVTYQDGTTSTVTVP
                     VKRVIPEIVAHSHYTVQGQDFPTGNGASASDYFKLSNGSAIPDATITWVSGQAPNKNN
                     TTIGQDINVTAHILIDGETTPITKTATYKVVRTVPKQVFETARGVLYPGVSDMYDAKQ
                     YVKPVNNSWSTNAQHMNFQFTNSYGPSKDVVGISTRDIRVTYDNHQTQIIKILSKVKP
                     DPPRIDGNSVTYKAGLTNQQIKINNVLSSSSIKLFKADNTPLTITNTTYGSGNTAVVT
                     VSDALPNGEIKARSSISMNNVTYTTQDEHGRAIDVTRNESVDSNDSASVHVTPQLQAT
                     TEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDNPDTWKNTVGNTHKTAVVTLPNG
                     QGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAINYITFDPNTNTNGITAVWANR
                     QQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTSYTTTVGGTLASGTQA
                     SGYAHIQNATGLPTDGFTYKWNNAATGTNDANWAAMNKPNTAQVINAKYDVIYNGHTF
                     ATSLPAKFVVKDVQPAKPTVTETAAGVITIAPGANQTVNTHAGNVTTYADKLVIKRNG
                     NVVTTFTRHNNTSPWVKEASAANVAGIAGTNNGITVAAGTFNPADTIQAVATQGSGET
                     ISDEQRSDDFTVVAPQPNQATTKIWQNGHVDITPNNPSGHLINPTQAMDIAYTEKVGN
                     GAEHSKTLNAVRGQNNQWTIANKPDYVTLDAHTGKVTFNANTIKPNSAITITPKAGTG
                     HSASSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGTAMPT
                     NLAGGSTTTIPVTVTYNDSSTEEVQESIFTKADKRELITAKNHLDDPVSTDGKKPGTI
                     TQYNNAIHNAQQQINTAKTEAQQVINDERATPQQVNAALSKVQAAQTKINEAKALLQN
                     KEDNSQLVTSKNNLQSSVNQVPSTTGMTQQSIDNYNAKKREAESEITAAQRVIDNGDA
                     TAQQISDEKHRVDNALTALNQAKQNLTADTHELEQAVHQLNRTGTTTGKKPASITAYN
                     NSIRALQSDLTSAKNSANAIIQKPIRSVQEVQTALTNVNRVNDRLTLAINQLVPLADN
                     SALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDATDQQ
                     IAAKKTKVEEKYNGLKQAIAGLTPDLAPLQAAKTQLQNDIDQPTSTTGMTSASVAAFN
                     DKLSAARTKIQEIDRVLASHPDVATIRQNVTAANATKTALDQARNGLTVDKAPLENAK
                     NQLQQSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVDQINTNTSAA
                     NQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQAARQ
                     KLTEINQVLNGNPTVQNINDKVAEANQAKDQLNTARQGLTLDRQPAFTTLHGASNLNQ
                     AQQNNFTQQINAAPNHAALETIKSNITALNNAMTKLKDSVADNNSIKSGQNYTDATTA
                     NKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAETVKSTKGALDGQQNLQRAKTEATN
                     AITHASDLNQTQKNALTQQVNNAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHNQVVQ
                     SDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEQALNGEA
                     KLNAAKQEANTALGQLNNLNNAQRQNLQSQINGAHQIETVNTIKQNATNLNSAMGNLR
                     QAVADKEQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVNDVNSATSAVT
                     TNKNALNGDEKLAQSKTDAASAIDALPHLNNAQKADVKSKINAASNIAGVNTVKQQGT
                     DLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQNKTKD
                     QVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTHLTNQINSGTTVA
                     GVHTAQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAETIIN
                     ANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKLNAKTYLNTLTSITDAQKNNLI
                     SQISSATRVSSVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQEYDN
                     AITAAKAILNKQHGPNTAQNAVEAALQRVKTAKNALNGDAKLIAAQNAAKQHLGTLTH
                     ITTAQRNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQNFLDA
                     DEQKRNAYNQAVSNAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNNAKQA
                     AITAINGASDLNQHQKDALKAQANGAQRVSNAQDVQRNATELNTAMGQLQHAIADKTT
                     TLASSKFVNADSTKQNVYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQELNG
                     DERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNNAMKG
                     LRDSIANETTVKASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDASAITQATTQ
                     VNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTAAKNA
                     ATELNTQMGNLEQAIHDQNTVKQGVNFTDADKAKRDAYTNAVSRAETILNKTQGANTP
                     KQDVEAAIQSVTSAKNALNGDQNVTNAKNAAKHALNNLTSINNAQKRDLTTKIDQATT
                     VSGVEAVSNTGTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTNAENI
                     LNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTAQKDK
                     LKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNAATKNGQNYLDATESNKTNY
                     NNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNAIDGA
                     TNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQTQKYR
                     DAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKLAEAK
                     AAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGSINDK
                     DATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRAKAAL
                     NGADNLRNAKTSATNTINGLPHLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTLNTAM
                     GALRTSIQNDNTTKTSQNYLDASDINKNNYNTAVNNANGVINATNNPNMDANAINGMA
                     NQVNTTKAALNGVQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDANNVQ
                     HTATELNGAMTVLKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGTPNAT
                     LTVNDVNSATSQVNAAKTVLNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQVQSA
                     TTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAVTAAK
                     AIINQTSNPTMEPNTITQATSQVTTKEQALNGAQNLAQAKTTAKNNLNNLTSINNAQK
                     DALTRSIDGATTVAGVNQETVKATELNNAMHSLQNGINDETQTKQTQKYLDAEPSKKS
                     AYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAKQTLG
                     TLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTLQSQN
                     YQDADDAKRTAYSQAVNAAATILNKTSGGNTPKADVERAMQAVTQANTALNGIQNLER
                     AKQAANTAITNASDLNTKQKEALKAQVTSAGRVSVANGVEHTATELNTAMTALKRAIA
                     DKADAKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPSDVTNAATQVTNAKT
                     QLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQTLNT
                     AMKGLRDSIANEATIKAEQNYTDASPNNRSEYDSAVTAAKAIIGQTSSPTMNAQEINQ
                     AKDQVTAKQQALNGQENLTNAQINAKQHLNGLSDLTNAQKDAAKRQIEGATHVSEVTQ
                     AQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILNKVQG
                     PNTAKDNVESALQNVQRAKNDLNGNQNVANAKTTAKNALNNLTSINNAQNEALKSQID
                     SATTVAGVNQVSATASELNTAMSNLQNGINDEAATKAAQKYTDADSDKQTAYNDAVTA
                     AKTLLDKTAGTNDNKAAVEQALQRVNTAKTALNGDARLNQAKNTAKQQLATMSHLTDA
                     QKANLTSQIERGTTVAGVQGIQANAGTLNEAMNQLRQSIASKDATKASEDYHDANTDL
                     QNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQTAKSD
                     IGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDNTKRS
                     VNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGDNKVA
                     QAKETAKRALASYSNLNNAQSTAATSQIDNATTVAGVTAAQNTANELNTAMGQLQNGI
                     NDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQVTTAK
                     NALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKAQDLD
                     GAMQRLQSAIANKDQTKASENYIDADPTNKTAFDNAITQAESYLNKDHGANKDKQAVE
                     QAIQSVTSTENALNGDANLQRAKTEATQAIDNLTHLNTPQKTALKQQVNAAQRVSGVT
                     DLKNSATSLNNAMDQLKQAIADHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIVNGSP
                     NVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSITVQI
                     DNATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDADRDKQNAYNTAVT
                     SAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINQLSHLNN
                     AQKQDLNTQVTNAPNISTVSQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTDADPE
                     KQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKNNANQ
                     TLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKDTTLG
                     SQNFADADPEKKNAYNEAVRNAENILNKSTGTNVSKDQVEAAMNQVNTTKAALNGTQN
                     LEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMDKLRQ
                     SIADNATTKQNQNYTDSSPNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAGQVST
                     TKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTDQINGAHTVDEANQIKQNAQN
                     LNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNATQTE
                     VEQAIQQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNATTVAG
                     VNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYKQAVAKAEALISG
                     TPDVVVTPSEITAALNKVTQAKNDLNGNTNLATTKQNVQHAIDQLPNLNQAQRDEYSK
                     QITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTAYDNA
                     VTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQAAKTNLGTLDHL
                     NDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLSNALQDKTETLNSINFTDAD
                     QAKKDAYTNAVAHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRAKDAA
                     KQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIADKDQT
                     KANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVTQAKGDLNGNHNLQVAK
                     DNANTAIDQLPNLNQTQKTALKDQVSHAELVTGVNAIKQNADALNNAMGTLKQQIQAN
                     SQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLTPDAVTQAVTTMNQAKDAL
                     NGDEKLAQAKQDAIANLDTLRDLNQPQRDALRNQINQAQALATVEQTKQNAQNVNTAM
                     SNLKQGIANKDTVKASENYHDADADKQTTYTNAVSQAEGIINQTTNPMLNPDDITRAL
                     TQVTDAKNGLNGEAKLATEKQNAKDAVNAMTHLNDAQKQALKGQIDQSPEIAIVNQVK
                     QTATSLDHAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKAEALLNKQSGTN
                     EVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQKQSFESQITQA
                     PLVTDVTTINQKAQALDHAMELLRNSVADNQATLASEDYHDATAQRQNDYNQAVTATN
                     NIINQTTSPTMNPDEVNRATTQVNNTKVALDGDENLVAAKQQANNRLDQLDHLNNAQK
                     QQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQTVEQLGNFVNADTDKQT
                     AYTTAVNEAEAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQVAKANATQAID
                     ALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRESIQDNAATKANSK
                     YINEDQSEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKAALHGDVKLQND
                     KDHAKQTVSQLTHLNNAQKHMEDTLIDSETTRTAVNHDLTEAQALDQLMDALQQSIAD
                     KDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVTSATQAVTSSKNA
                     LDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAEIIAQAQALNEA
                     MKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTDPTLAKSIIDQA
                     TQAVTDAKNNLHGDQKLDQDKQRATETLNNLSNLNTPQRQALENQINNAATRGEVAQK
                     LTEAEALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNNP
                     TLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQINN
                     AATRDEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQNA
                     KDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQLQAVTTVNALPNLNHAQ
                     QQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDKK
                     EAVDQALQAAESITDPTNGSNANKDAVDQALTKLQEKVNELNGNERVAEAKTQAKQTI
                     DQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQTV
                     DYTQADLDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAKT
                     NGKHDIEQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRVMDQLSQGITGNE
                     GRTKGSTNYVNADTQVKQVYDEAVDKAKQALDKSTGQNLTAEQVIKLNDAVTAAKKAL
                     NGEEKLNNRKSEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNAM
                     GAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQL
                     KQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQHLAHNAINNADTVSDVTDI
                     VNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNAEDNAKTNFDDAKRLANTLLNSDNT
                     NVNDINGAIQTVNDAIQNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDKL
                     IAKNKAEELANSIINNINKATSNQDVSQVQTAGNHAIEQVHANEIPKAKIDANKDVDK
                     QVQALIDEIDRNPILTDKEKQALKDRINQILQQGHNDINNAMTKEEIEQAKAQLGQAL
                     QDIKDLVKAKESAKQDIDKQVQALIDEIDRNPILTDKEKQALKDRINQILQQGHNGIN
                     NAMTKEEIEQAKAQLGQALQDIKDLVKAKESAKQDIDKQVQALIDEIDRNPNLTDKEK
                     QALKDRINQILQQGHNGINNAMTKEEIEHAKAQLAQALQDIKDLVKAKEDAKNAIKAL
                     ANAKRDQINSNPDLTLEQKAKALKEIDEAEKRALENIENAQTKDQLNQGLNLGLDDIR
                     NTHVWEVDAQPAVNEIFDATPEQILVNGELIVHRDDIITEQDILAHINLIDQLTAEII
                     DTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELTVVKQQAIESIENAAQQKI
                     NEINNHATLTPEQKEAAIAEVNKLKQQAIEQINNAADVHTVEEVQHQEQAHIEQFNPD
                     QFTIDQAKSNAIKSISDAIQHMIDEINASKDLTDKEKQEAISKLNQLKDQSIQAIQRA
                     QSIDEIAQQLEQFKAQLKAANPFAKELENRKKAAISKIKDISTDKIDRIRNSTIGTAE
                     ERQAAMNRINEIVLETIKDINNAQTPQQVEAALNNGIARILAVQIVTSDHSKPSSNSD
                     GQSNSHLHVGYGTVNHPFNSSPIGHKKKLDQDDEIDPLHMRHFGDRIGNVIKNALGVV
                     GISGLLASFWFFIAKRRRKEDEEEELEIRDNSKDKKKGSIEGTKHLPLLFAKRRRKED
                     EEDAIVEEKDSLNNDESLDKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLQDNEHS
                     PVLIAKRLKDKDGNVETTTSIESKDEDVPLLLAKKKNQKDNQSKGKKSASKKPSKKVA
                     AKKKKKKSKKNKK"
     gene            complement(32137..32538)
                     /locus_tag="VB241_000397"
     CDS             complement(32137..32538)
                     /locus_tag="VB241_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEP1243729.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQHAQQALYKFIKKNK"
     gene            complement(32779..33483)
                     /locus_tag="VB241_000398"
     CDS             complement(32779..33483)
                     /locus_tag="VB241_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEP1243730.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTAIFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            33728..33922
                     /locus_tag="VB241_000399"
     CDS             33728..33922
                     /locus_tag="VB241_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEP1243731.1"
                     /translation="MSRILTYSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            33934..34185
                     /locus_tag="VB241_000400"
     CDS             33934..34185
                     /locus_tag="VB241_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HEP1243732.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            34223..35347
                     /locus_tag="VB241_000401"
     CDS             34223..35347
                     /locus_tag="VB241_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HEP1243733.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEDILPDIKAAFSGESQVLESGKDPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTKHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMTNRTI"
     gene            35363..35800
                     /locus_tag="VB241_000402"
     CDS             35363..35800
                     /locus_tag="VB241_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEP1243734.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEEVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGRDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            36224..37180
                     /locus_tag="VB241_000403"
     CDS             36224..37180
                     /locus_tag="VB241_000403"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP1243735.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            37380..37859
                     /locus_tag="VB241_000404"
     CDS             37380..37859
                     /locus_tag="VB241_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP1243736.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            37874..38713
                     /gene="fakB2"
                     /locus_tag="VB241_000405"
     CDS             37874..38713
                     /gene="fakB2"
                     /locus_tag="VB241_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HEP1243737.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVAHANVIEYVDKLKKVFNEAFHVDNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            38799..39332
                     /gene="msrA"
                     /locus_tag="VB241_000406"
     CDS             38799..39332
                     /gene="msrA"
                     /locus_tag="VB241_000406"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP1243738.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHADNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DKHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            39325..39753
                     /gene="msrB"
                     /locus_tag="VB241_000407"
     CDS             39325..39753
                     /gene="msrB"
                     /locus_tag="VB241_000407"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP1243739.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            39765..40265
                     /locus_tag="VB241_000408"
     CDS             39765..40265
                     /locus_tag="VB241_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEP1243740.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            40265..40486
                     /locus_tag="VB241_000409"
     CDS             40265..40486
                     /locus_tag="VB241_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP1243741.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT        16115 a         7056 c         7279 g        10037 t
ORIGIN      
        1 tttacctacc aatatatttt gtgttattat attctggaga ataaataaat tgttcaacac
       61 atagttgtaa tgtatttcaa tactttttag atagattgcg aaattgtatt gaatcgtcat
      121 tgttttaaat ttttaaatga gaatggaatg agcattacaa tacacatgaa atcaaatgta
      181 aatacattca caacacaaca gagacataac aacaagataa ggagtgaaca atagctgtga
      241 attatcgtga taaaattcaa aagtttagta ttcgtaagta taccgttgga acattctcga
      301 cggtcattgc gacgttagta tttctaggat taaacacatc acaagcacaa gctgctgaga
      361 caaatcaacc agctagtgca ttaaaacaga aacaacaaaa tggtgatacg cagactgaga
      421 atcgagaagt agaagttcaa aattcacaaa atggtcaatc attatctgcg cctattgaaa
      481 atgaacaacc aaataataat caaacaaacc atgtagatgc tagtgcagta caatcatcta
      541 caacagaaca tgaccaacca gtatctcaaa atgagcaagc aaagaaagat acggcagctg
      601 ctacgccaac acaatcggct aaagcggcaa gtaagcatga acaaagcgaa agtcgcgctg
      661 ctaacaaaaa ggaaaacgac aataaagcga cacatgtaga aagtcatgaa gcaaatgtag
      721 tcacagcttc agattcatca gatagtggta acgtacaaca cgatcgtaat gaattacaag
      781 cattttttga tgctaattac catgattatc gctttattga ccgcgaaaat gcagattctg
      841 ggacatttaa ctatgtaaaa ggcatttttg acaagatcaa tacgttatta ggtagtaatg
      901 atccaataaa caataaagac ttgcaacttg catacaaaga attagaacaa gctgttgctt
      961 taattcgtac aatgcctcaa cgtcaacaaa caagtcgacg ctcaagtaga attcaaacgc
     1021 gttcgattga atcaagagct gcagaaccaa gatcagtatc agactatcaa aacgcaaact
     1081 catcatatta cgttgaaaat gctaatgatg gttctgggta tcctgtaggt acatatatta
     1141 atgcttctag taagggggca ccatataatt tacctactac gccatggaat acgttaaaag
     1201 catcagacgc aaaagaaatc gcacttatta cagcaaagca aactggagac ggttatcaat
     1261 gggtgattaa gtttaacaaa ggtcatgcac cacatgaaaa tatgatctac tggtttgcac
     1321 taccggccgg acaaactcca gttggtcgaa cggagtttgt aacagtgaat gcagacggca
     1381 caaatgtaca atggagtaac ggtgcaggag ccggtgcgaa caaaccactt ccagaaatgt
     1441 ggccatatgg ggtaaacgac tcacgttcgt gggactacaa aattagaaat aggagtgggc
     1501 aggtgattta tgattggcca actgtccata ttaatagctt aaaggattta gcacgtgcaa
     1561 gtgactattt tagtgaagct ggagcaacac ctgctacaaa agcttttggt agacaaacat
     1621 tcgaatatat taatggtgaa aggccgacag aatcacctgg tgtaccaaaa gtttatacat
     1681 ttattggtaa aggtgatgca agttatacga tttcatttaa aacgcaaggt ccaacaattg
     1741 ataagctgta ctatgcagca ggtgggcgtg ctttagaata caatcaatta tttatgtata
     1801 gtcagttata tgtcgaatca acacaagact atcaacaacg tcttaatggt ttaagacaag
     1861 tggttaatcg tacttatcgt ataggtacaa ctaaacgagt agaagtgagt caaggaaatg
     1921 tacaaacaaa aaaggtatta gaaagtacga acctaaatat agatgacttt atggatgatc
     1981 cattaagtta tgttaagaca ccaagtaata aagtattagg tttttatcca actagtgcaa
     2041 acactaacgc ttttagaccg ggtggagtca atccattaaa tgaatatcaa ttaagtcaat
     2101 tatttactga tgataaattg caacaggcag ctagaactgg cagcccaata cgattaatga
     2161 ttggtttcga ctatcctgat gcttttggta acggtgaaac tttagtgcct gttaacttaa
     2221 cagtattacc tgaaattcaa cataacatta aattctttaa aaatgatgac ggtcaaaata
     2281 ttgctgataa acctgcttca aaacaagctg gacatccggt tttctatgta tatgcaggta
     2341 atcaagggaa tgcttcagta aacttaggtg gtagtgtaac atctatacaa ccgttacgta
     2401 ttaatttaac aagtaatgaa aactttacag ataaagattg gcaaattaca ggtattccac
     2461 gtacattaca cattgagaat tcaacaaata gaactaataa tgctagagaa cgtaatattg
     2521 aactggttgg taatttatta ccaggcgatt actttggaac aatacgattt ggacgtaaag
     2581 aacaattatt cgagattcgt gttaagccac atacgcctag aattactaca acagcagaag
     2641 aattaagagg tacagcatta caaaaggtac ctgtaaccgt tacggatatt ccattggatc
     2701 catctgcatt agtatattta gttataccta cgtctcaaac aagagatggt ggttctgagg
     2761 cagatcaaat accatcaggt tatacaaaaa ttgcgacggg tacacctgat ggggttcatt
     2821 caacgataac gatacgacca gaagattatg tagtatttat acctccggta ggtaatcaaa
     2881 ttagggcgtt aatattttac aataatgtag ttgcctcaaa tatgagtaat gcagtaacta
     2941 ttttacctga tgatattcca ccgacaatta ataacccagt tggattaaat gcgaaatatt
     3001 atcgtggtga tgaagttagc tttactatgg gggtttcaga tagacattct ggattaaaaa
     3061 gtacaactat tacgacattg ccaagtggtt ggacatcaaa tttaactaaa tccgacaaga
     3121 aaaacggctc attagctatt tcaggtagag tctctatgaa tcaggcctat aatagtgata
     3181 ttacattcaa agtgtcagca accgacaatg tcaataatac gacgaatgat agtcagtcta
     3241 aacatgtgac agtgcatgta ggtaaaatta gtgatgatgc gcatccaatt gtcttaggaa
     3301 attctgaaaa agttgtcgtt gtaaatccga ctgctttaac tggtgatgaa aagcaacgta
     3361 ttactactgc gtttatgaat aaaaaccaaa atataagagg ttatttagct tcatcaaatc
     3421 cagttacagt agataatcat ggtaatgtca cattgcaata tcgtgatggg tcatcaacta
     3481 cgcttgatgc gacaaacgtg atgacatacg aaccagttgt taagcctgaa tatcaaactg
     3541 ctaatgctgc taaaacggca acggttacaa ttgctaaagg gcaatctttt aatattggag
     3601 atatcaaaca atatttcact ttaagtaatg gacaagcgat tccaagcagt tcatttacaa
     3661 acattacatc ggatagaacg attccaactg cgcaagaagt tagtcaaatg aacgctggca
     3721 cacagctata tcatatagtt gctacaaatg cgtatcataa agacactgaa gatttctata
     3781 tcactttgaa aatcattgat gtgaaacaac cagaaggtga tcaacgtgta tatcgtatgt
     3841 caacatatga tataactact gatgaaattt caaaagtgaa acaagcattt atcaatgcca
     3901 atagagatgc gatttcattt gcagaaggag atatttcagt aacaaataca ccgaatggtt
     3961 ctaatgtgag taccattact gtcaatataa ataaaggtcg attaacgaaa tcgttcacat
     4021 caaatcttaa taatatgaac ttcttacgtt gggttaactt ccctcaagat tatacagtga
     4081 catggacaaa cgcaaaaatt gcaaatagac cgactgatgg tggtttatca tggtctgatg
     4141 atcataaatc attaatttat cgctatgatg caacattagg tacgcaaatt acgacgaatg
     4201 atattttaac tttgttaaaa gcgacaacaa cggtgcctgg attacgtaat aacattgctg
     4261 gtaatgaaaa agcacaagca gaagctggtg gtagaccaaa ctataaaaca actggttatt
     4321 cacaatcaaa tccaacatct gatggtcaac gacaatttac gttgaatggt caagtcattc
     4381 aaataatgga tattattaat ccttcaaatg gatttggtgg gcaacctgtt acaaattcaa
     4441 atgtccgtgc gaaccacagt aactcgactg tcgttagtgt taatgaatca gcagctaatg
     4501 gtgcgggtgc atttacaata gatcatgtag taaaaaataa ttctacgcat aatgcagctg
     4561 atgcagttta taaagcgcag ttatacttat cgccatatgg accaaaacaa tatgttgaac
     4621 atttaaatca aaatacggac aatacgaatg aagcaattaa catttatttt gtacctagtg
     4681 atctagtaaa tccaacgatt tcagtaggta attacacaaa tcatcaagta ttctctggtg
     4741 aaacatttac caatacgatt acagcgaatg ataattttgg tgtgcaatct gtaacagtac
     4801 caacgacatc acaattgaca ggcactgttg ataataatca tcaacatgtt tcagcaacag
     4861 caccaaatgt gacatctact acaaataaaa caattaattt ggttgcaact gatacaagcg
     4921 gtaatactgc aacaacgtca ttcaatgtca caattaaacc tttacgtgat aaatatcgag
     4981 ttggaacatc atcaacggct gctaatccag tgagaattgc caatatttcg aataatgcga
     5041 cagtatcgca agctgatcaa acagcaatta ttaattcatt aacgtttaca agcaatgcac
     5101 caaatagaaa ctatgcaaca gcaagtgcaa atgaaatcac tagtaaaaca gtgagtaatg
     5161 tgagtcgtac aggaaataac gcacaagtaa ctgtaactgt tacttatcaa gatggaacaa
     5221 catcaacagt gacagtacct gtaaagcgtg ttattccaga aatcgttgca cattcgcatt
     5281 acactgtaca agggcaagac ttcccaacag gtaatggagc aagtgcatct gattacttta
     5341 aattatcgaa tggtagtgcc attccagatg cgacgataac atgggtaagt ggtcaagcgc
     5401 caaacaaaaa taacacaact ataggtcaag atatcaatgt aactgcacat atcttaattg
     5461 atggtgaaac aaccccaatt acgaaaacag caacatataa agtggtaaga actgtaccaa
     5521 agcaagtctt tgaaacagcc agaggtgtct tatacccagg tgtttcagat atgtatgatg
     5581 cgaaacaata tgttaagcca gtaaataatt cttggtcgac aaatgcgcaa catatgaatt
     5641 tccaatttac aaattcatat ggaccaagta aagatgtagt aggtatttca acaagggata
     5701 tccgagttac ttatgataat caccaaacac aaattatcaa gattttatct aaagttaagc
     5761 ctgatccacc gagaattgac ggaaactctg tgacatacaa agcaggctta actaaccaac
     5821 aaatcaagat taataatgtt ttaagtagtt cttctattaa attatttaaa gcagataata
     5881 caccattaac aattactaac acgacttatg gtagtggaaa tactgcagta gttactgtaa
     5941 gtgatgcgtt accaaatggt gaaattaaag caagatcttc gatttcaatg aataatgtaa
     6001 cttatacgac acaagacgaa catggacgag ctatagatgt aacaagaaat gaatctgttg
     6061 attcaaatga cagtgcttct gtacatgtga cacctcaatt acaagcgact actgaaggcg
     6121 ctgtatttat taaaggtggc gacggttttg atttcggtca tgtagaacga tttattcaaa
     6181 atccaccaca tggggcaacg gttgcatggc atgataatcc agatacatgg aagaatacag
     6241 tcggaaacac tcataaaact gcggttgtaa cattaccaaa tggtcaaggg acacgtaatg
     6301 tagaagtacc tgtcaaagtt tatccagttg ctaatgccaa agcgccatct cgtgatgtta
     6361 aaggtcaaaa tttgactaat ggaacggatg cgataaacta cattacattt gatccaaata
     6421 caaacacaaa tggtatcact gcagtatggg caaatagaca acaaccaaat aaccaacaag
     6481 caggcgttca acatttaaat gtcgatgtaa catatccagg tatttcagct gcaaaacgtg
     6541 tacctgttac tgtgaatgta tatcaatttg aattccctca aacttcttat acaacaacag
     6601 ttggtggcac tttagcaagt ggtacgcaag catcaggata tgcacatata caaaatgcta
     6661 ctggtttacc aacagatgga tttacgtata aatggaataa tgcagcaaca ggtacaaacg
     6721 atgcaaactg ggcagcaatg aataaaccaa atactgcaca agtcattaat gcaaaatatg
     6781 atgtcatcta taatggacat acatttgcaa catctctacc agcgaagttt gtagtaaaag
     6841 atgttcaacc agcgaaacca actgttacag aaacggcagc aggagtaatt acgattgcac
     6901 ctggagcaaa ccaaacagtg aatacacatg ccggtaacgt aacgacatac gctgataaat
     6961 tagttattaa acgtaatggt aatgttgtga cgacatttac tcgtcataat aatacgagtc
     7021 catgggtgaa agaagcatct gcagcaaatg tagcaggtat tgctggaacg aataatggta
     7081 ttactgttgc agctggtact ttcaatcctg ctgatacaat tcaagctgtt gcaacacaag
     7141 gtagtggtga aacaatcagt gacgaacaac gtagtgatga tttcacagtt gtcgcacctc
     7201 aaccgaacca agcgactact aagatttggc aaaatggtca tgttgatatc acgcctaata
     7261 acccgtctgg acatttaatt aatccaactc aagcaatgga tattgcttac actgaaaaag
     7321 tgggtaatgg tgcagaacat agtaagacac ttaatgctgt tcgtggtcaa aataatcaat
     7381 ggacaatcgc caataaacct gactatgtaa cgctagatgc acatactggt aaagtgacgt
     7441 tcaatgccaa tactattaag ccaaattcag caatcaccat tacaccgaaa gcaggtactg
     7501 gtcactcagc aagcagtaat ccaagtacat taactgcacc ggcagcacat actgtcaaca
     7561 caactgaaat tgtaaaagat tatggttcaa atgtaacagc agctgaaatt aacaatgcag
     7621 ttcaagttgc taataaacgt actgcaacga ttaaaaatgg cacagcaatg cctactaatt
     7681 tagctggtgg tagtacaacg acgatccctg tgacagtaac ttacaatgat agcagtactg
     7741 aagaagtaca agagtccatt ttcacaaaag cggataaacg tgagttaatc acagctaaaa
     7801 atcatttaga tgatccagtg agtacagatg gtaaaaagcc aggtacaatt acgcagtaca
     7861 ataatgcaat tcataatgcg caacaacaaa tcaatactgc gaaaacagaa gcgcaacaag
     7921 tgattaatga tgagcgtgca acaccacaac aagtgaacgc agcattatct aaagttcaag
     7981 ctgcacaaac taagataaat gaagctaaag cattacttca aaataaagaa gataatagcc
     8041 aattagtaac atctaaaaat aacttacaaa gttctgtgaa ccaagtacca tcaactactg
     8101 gtatgacgca acaaagtatt gataactata atgcgaagaa acgtgaagca gaatctgaaa
     8161 ttacagcagc tcaacgtgtc attgataatg gtgacgcaac agcacaacaa atttcagatg
     8221 aaaaacatcg tgtcgataac gcattaacag cattaaacca agcgaaacaa aatttaacag
     8281 cagatacaca tgaattagag caagcagtac atcaattgaa tcgtacaggt acaacgacag
     8341 gtaagaaacc agcaagtatc actgcataca ataattcgat tcgtgcactt caaagtgact
     8401 taacaagtgc taaaaatagt gccaacgcta ttattcagaa gccaattaga tcagtgcaag
     8461 aggtacaaac agcattaacg aatgtaaatc gtgtaaatga ccgattaacg ctagctatta
     8521 atcaattagt acctttagct gataatagtg ctttaagaac tgctaaaacg aaacttgatg
     8581 aagaaatcaa taaatcagta actactgatg gtatgacaca atcatcaatc caagcatatg
     8641 aaaatgctaa acgtgcgggt caaacagaat caacaaatgc acaaaatgtt attaacaatg
     8701 gtgacgcaac agaccaacaa attgccgcaa aaaaaactaa agtagaagaa aaatataatg
     8761 gcttaaaaca agctattgcc ggattaacac cagacttggc accattacaa gctgcgaaaa
     8821 ctcagttgca aaatgatatt gatcaaccaa cgagcacgac aggtatgaca agtgcatcag
     8881 ttgctgcatt taatgacaaa ctttcagcag ctagaactaa aattcaagaa attgatcgcg
     8941 tactagcatc gcatccagat gttgcaacaa ttcgacaaaa cgtgacagca gcgaatgcta
     9001 ctaaaacagc acttgatcaa gcacgcaatg gcttaacagt agataaggcg cctttagaaa
     9061 atgcgaaaaa tcaactacaa caaagtattg atacgcaaac aagtacaact ggtatgacgc
     9121 aagattctat taatgcatac aatgcgaagt taacagctgc acgtaataag attcaacaaa
     9181 tcaatcaagt attagctggt tcacctactg tagatcaaat taatacaaat acgtctgcag
     9241 caaatcaagc gaaatctgat ttggatcatg cgcgtcaagc tttaacacca gataaagcac
     9301 cgcttcaaac tgcgaaaacg caattagaac aaagcattaa tcaaccaacg gatacaacag
     9361 gtatgacgac cgcttcgtta aatgcgtaca accaaaaatt acaagcagcg cgtcaaaagt
     9421 taactgaaat taatcaagtg ttgaatggca acccaacggt ccaaaatatc aatgataaag
     9481 tggcagaggc aaaccaagct aaggatcaat taaatacagc acgtcaaggt ttaacattag
     9541 atagacaacc agcgtttaca acattacatg gtgcatctaa cttaaaccaa gcacaacaaa
     9601 ataatttcac gcaacaaatt aatgctgcac caaatcatgc tgcacttgaa acgatcaagt
     9661 ctaacattac agctttaaat aatgcgatga caaaattaaa agacagtgtt gcggataata
     9721 attcgattaa atcaggtcaa aattacactg acgcaacaac ggctaataaa caagcgtatg
     9781 acaatgcagt taatgcagct aaaggtgtca ttggagaaac gactaatcca acgatggatg
     9841 ttaatacagt gaaccaaaaa gcagaaacag tgaaatctac aaaaggcgct ttagatggtc
     9901 aacaaaactt acaacgtgcg aaaacagaag caacaaatgc gattacgcat gcaagtgatt
     9961 taaaccaaac acaaaagaat gcattaacac aacaagtaaa taatgcacaa aatgtacaag
    10021 cagtaaatga tattaaacaa acgactcaaa gcttaaatac tgctatgaca ggtttaaaac
    10081 gtggcgttgc taatcataac caagtcgtac aaagtgataa ttatgtcaac gcagatacta
    10141 ataagaaaaa tgattacaac aatgcataca accatgcgaa tgacattatt aatggtaatg
    10201 cacaacatcc agttataaca ccaagtgatg ttaacaatgc tttatcaaat gtcacaagta
    10261 aagaacaggc attgaatggt gaagctaagt taaatgctgc gaagcaagaa gcgaacacag
    10321 cattaggtca attaaataac ttgaataatg cacaacgtca aaacttacaa tcgcaaatta
    10381 atggtgcgca tcaaatcgag acagttaata cgattaagca aaatgcaact aacttaaata
    10441 gtgcaatggg taacttaaga caagctgttg cagataaaga gcaagtgaaa cgtacagaag
    10501 attatgcgga tgcagataca gctaaacaaa atgcatataa tagcgccgtt tcaagtgctg
    10561 aaacaatcat taatcaaaca acaaatccaa cgatgtctgt taatgatgtt aacagtgcaa
    10621 cttcagctgt tactactaat aaaaatgcat taaatggtga tgaaaaatta gcacaatcta
    10681 aaacagatgc tgcaagtgca attgatgcat taccacattt aaataatgct caaaaagcag
    10741 atgttaaatc taaaattaat gctgcatcaa atattgctgg tgtaaatact gttaaacaac
    10801 aaggtacaga tttaaataca gcgatgggta acttgcaagg tgcaatcaat gatgaacaaa
    10861 cgacattaaa tagtcaaaat tatcaagatg cgacaccaag taagaaaaca gcatacacaa
    10921 atgcggtaca agcagcgaaa gatattttaa acaaatcaaa tggtcaaaat aaaacgaagg
    10981 atcaagttac agaagcgatg aatcaagtga attcggctaa aaataactta gatggtacgc
    11041 gtttattaga tcaagcaaag caaacagcga aacagcagtt aaataatatg acgcatttaa
    11101 caactgctca aaaaacgcat ttaacaaatc aaattaatag tggtactact gtcgctggtg
    11161 ttcatacggc tcaatcaaat gcgaatacat tagatcaagc gatgaatacg ttaagacaaa
    11221 gtattgccaa caaagatgcg actaaagcaa gtgaagatta cgtagatgct aataatgata
    11281 agcaaacagc atataacaac gcagtagctg ctgccgaaac gattattaat gcgaatagta
    11341 atccagaaat gaatccaagt acgattacac aaaaagcaga gcaagtgaat agttctaaaa
    11401 cggcacttaa cggtgatgaa aacttagcta cggcaaaact aaatgcaaaa acatacttaa
    11461 acacattgac aagtattact gatgctcaaa agaacaattt gattagtcaa attagtagtg
    11521 cgacaagagt gagtagtgtt gatactgtaa aacaaaatgc gcaacatcta gaccaagcta
    11581 tggctagctt gcaaaatggt attaacaacg aatctcaagt gaaatcatct gagaaatatc
    11641 gtgatgctga cacaaataaa caacaagagt atgataatgc tattactgca gctaaagcga
    11701 ttttaaataa acaacatggt ccaaacactg cgcaaaatgc agttgaagca gcattgcaac
    11761 gtgtgaaaac tgcgaaaaat gcattgaatg gtgatgccaa attaattgca gctcaaaacg
    11821 cagcgaaaca acatctaggt actttaacgc atatcactac agcacaacgc aatgatttaa
    11881 caaatcaaat ttcacaagct acaaacttag ctggtgttga atctgttaag caaagtgcga
    11941 atagtttaga tggtgctatg ggtaacttac aaacggctat caacgataag tcaggaacat
    12001 tagcgagcca aaacttccta gatgctgatg agcaaaaacg taatgcttac aatcaagctg
    12061 tatcaaatgc cgaaacaatt ttaaataaac aaactggacc gaatacagcg aaaactgcag
    12121 tcgaacaagc acttaataat gttaatagtg cgaaacatgc attaaatggt acgcaaaact
    12181 taaataatgc gaaacaagca gcgattacag caatcaatgg cgcatctgat ttaaatcaac
    12241 atcaaaaaga tgcattaaaa gcacaagcta atggtgctca acgcgtatct aatgcacaag
    12301 atgtacaacg taatgcgact gaactgaaca cggcaatggg tcaattacaa catgccatcg
    12361 cagataagac gactacgtta gcaagcagta aatttgttaa cgccgatagc actaaacaaa
    12421 atgtttacac aactaaagtt accaatgctg aacatattat tagcggtacg ccaacggttg
    12481 ttacgacacc ttcagaagta acagctgcag ctaatcaagt aaacagcgca aaacaagaat
    12541 taaatggtga cgaaagatta cgtgttgcaa aacaaaacgc caatactgct attgatgcat
    12601 taacgcaatt aaatacacct caaaaagcta aattaaaaga acaagtggga caagccaata
    12661 gattagaaga cgtacaatct gttcaaacaa atggacaatc attgaacaat gcaatgaaag
    12721 gcttaagaga tagtattgct aacgaaacaa cagtcaaagc aagtcaaaac tatacagacg
    12781 caagtccgaa taaccaatca acatacaata gcgctgtgtc aaatgcgaaa ggtatcatta
    12841 atcaaactaa caatccgact atggatgcta gtgcgattac ccaagctaca acacaagtga
    12901 ataatgctaa aaatggttta aacggtgctg aaaacttaag aaatgcacaa aacactgcta
    12961 agcaaaactt aaatacgtta tcacacttaa caaataacca aaaatctgca atctcatcac
    13021 aaattgatcg tgcaggtcat gtgagtgagg taacagctgc taaaaatgca gcaactgagt
    13081 taaacacgca aatgggcaac ttggaacaag ctatccatga tcaaaacaca gttaaacaag
    13141 gtgttaactt cactgatgca gataaagcca aacgtgatgc gtatacaaat gcggtaagca
    13201 gagcagaaac aattctgaac aaaacgcaag gtgcaaatac gcctaaacaa gatgtagaag
    13261 cggctattca aagtgttact agcgctaaaa atgcattgaa tggtgatcaa aatgtcacaa
    13321 atgcgaagaa tgcagctaaa catgcattaa ataatttaac ttcaattaat aatgcacaaa
    13381 aacgtgactt aacaactaaa attgatcaag caacaacagt atctggtgtt gaagcggtat
    13441 ctaatacagg tacgcaattg aatacagcga tggctaactt gcaaaatggt attaatgata
    13501 aaacgaatac tttagcgagc gaaaactatc atgatgctga ttcagataag aaaactgctt
    13561 atactcaagc cgttacgaac gcagaaaata ttttaaataa aaatagtgga tcaaatttag
    13621 acaaagctgc cgttgaaaac gcgttgtcac aagtgacaaa tgcgaaaggt gccctaaatg
    13681 gtaaccataa tttagagcaa gctaaatcaa atgcaaacac tactataaac ggacttcaac
    13741 atttaacaac tgctcaaaaa gataaattga aacaacaagt gcaacaagca caaaatgttg
    13801 caggtgtaga tactgttaaa tcaagtgcca acacattaaa tggtgctatg ggtacgttaa
    13861 gaaatagcat tcaagataac gcagctacga aaaatggcca aaactatctt gatgctacag
    13921 aaagtaacaa aacaaactat aacaatgctg ttgatagtgc taatggtgtc attaatgcaa
    13981 caagcaatcc aaatatggat gctaatgcaa ttaaccaaat cgctacacaa gtgacatcaa
    14041 cgaaaaatgc attagatggt acacataatt taacgcaagc gaaacaaaca gcaacaaatg
    14101 cgattgatgg tgctactaac ttaaataaag cgcaaaaaga tgcgttaaaa gcacaagtta
    14161 caagtgcgca acgtgttgca aatgtaacaa gtatccaaca aactgcaaat gaacttaata
    14221 cagctatggg tcaattacaa catggtattg atgatgaaaa tgcaacaaaa caaactcaaa
    14281 aatatcgtga tgcagagcaa agtaagaaaa ctgcttatga tcaagctgta gctgctgcga
    14341 aagcaatttt aaataaacaa actggatcaa attcagataa agcagcagtt gaccgtgcat
    14401 tacaacaagt aacaagtacg aaagatgcat tgaatgggga tgctaaactg gcagaagcga
    14461 aagcggcagc taaacaaaac ttaggcactt taaaccatat tacgaatgca caacgtactg
    14521 acttagaagg ccaaatcaat caagcgacga ctgttgatgg cgttaatact gtgaaaacaa
    14581 atgccaatac attagacggc gcaatgaata gcttgcaagg ttcaatcaat gataaagatg
    14641 cgacattaag aaatcaaaat tatcttgatg cggatgaatc aaaacgaaat gcatatacgc
    14701 aagctgtcac agcggctgaa ggcattttaa ataaacaaac tggtggtaac acatctaaag
    14761 cagacgttga taatgcatta aatgcagtta caagagcgaa agcggcttta aatggtgctg
    14821 acaacttaag aaatgcgaaa acttcagcaa ctaatacgat taatggttta ccacacttaa
    14881 cacaattaca aaaagacaac ttgaagcatc aagttgaaca agcgcaaaat gtagctggtg
    14941 taaatggtgt taaagataaa ggtaatacat taaatactgc catgggtgca ttacgtacaa
    15001 gtatccaaaa tgataatacg acgaaaacaa gtcaaaatta tcttgatgca tctgatatca
    15061 acaaaaataa ttacaatact gctgtaaata atgcaaatgg tgttattaat gcaacgaaca
    15121 atccaaatat ggatgctaat gcgattaatg gcatggcaaa tcaagtcaat acaacaaaag
    15181 cagcgttaaa tggtgtacaa aacttagctc aagctaaaac aaatgcgacg aacacaatta
    15241 ataacgcaca tgacttaaat caaaaacaaa aagatgcatt aaaaacacaa gttaacaatg
    15301 cacaacgtgt atctgatgca aataacgttc aacatacagc tactgaattg aacggcgcga
    15361 tgacagtact taaagcagct attgctgata aagaaagaac aaaagcaagc ggaaattatg
    15421 tcaatgctga tcaagaaaaa cgtcaagcgt atgattcaaa agtgactaac gccgaaaata
    15481 tcattaatgg tacaccgaat gcgacattaa cagtcaatga cgtaaatagt gcaacttcac
    15541 aagtgaatgc agctaaaaca gtattaaatg gtgataacaa cttacgtgtt gcgaaagagc
    15601 atgccaacaa tacaattgat ggcttagcgc aattgaataa tgcacaaaaa gcaaaattaa
    15661 aagaacaagt tcaaagtgca actacattag atggcgttca aactgttaaa aatagttctc
    15721 aaacgttgaa tacagcgatg aaaggcttaa gagatagtat tgcgaatgaa gcaacaatta
    15781 aagcaggtca aaactacact gacgcaagtc caaataatcg taacgagtac gacagcgcag
    15841 ttactgcagc aaaagcaatc attaatcaaa catcgaaccc aacgatggaa ccaaatacta
    15901 ttacgcaagc aacatcgcaa gtgacaacta aagaacaagc attaaatggt gcgcaaaact
    15961 tagctcaagc taagacaact gcgaaaaaca acttgaataa cttaacatca attaacaatg
    16021 cacaaaaaga tgcgttaacg cgtagcattg atggtgcaac aacagtagct ggtgtaaatc
    16081 aagaaactgt caaagcaact gaattaaata acgcaatgca cagtttacaa aatggtatca
    16141 atgatgagac acaaacaaaa caaactcaga aatacctaga tgcagagcca agtaagaaat
    16201 cagcttatga tcaagcagta aatgcagcga aagcaatttt aacaaaagct agtggtcaaa
    16261 atgtagacaa agcagcagtt gaacaagcat tacaaaatgt gaatagtact aagacagcgt
    16321 tgaacggtga tgctaaatta aatgaagcta aagccgctgc gaaacaaacg ttaggtacat
    16381 taacacacat taataatgca caacgtacag cgttagataa tgaaattaca caagcaacaa
    16441 atgttgaagg tgttaataca gttaaagcca aagcgcaaca attagatggt gctatgggtc
    16501 aattagaaac atcaattcgt gataaagaca cgacgttaca aagtcaaaat tatcaagatg
    16561 ctgatgatgc gaaacgaact gcttattctc aagcagtaaa tgcagcagct acaattttaa
    16621 ataaaacatc tggcggtaat acacctaaag cagatgtcga aagagcaatg caagctgtta
    16681 cacaagccaa tactgcatta aacggtattc aaaacttaga acgtgcgaaa caggctgcta
    16741 acacagcgat tacaaatgct tcggacttaa atacaaaaca aaaagaagca ttgaaagcac
    16801 aagtaacaag tgcaggacgc gtatctgtag caaatggtgt tgaacatact gctactgaat
    16861 taaatactgc gatgacagct ttaaaacgtg ccattgctga taaagctgac gcaaaagcta
    16921 gtggtaacta tgtcaatgct gatgcgaata aacgccaagc atatgatgaa aaagtgacag
    16981 ctgcagaaaa tatcgttagt ggtacaccaa caccaacgtt aacaccatca gatgttacaa
    17041 acgcagcaac gcaagtaacg aatgcgaaga cgcagttaaa cggtaatcat aatttagaag
    17101 tagcgaaaca aaatgccaac acagcaattg atggtttaac ttctttaaat ggtccgcaaa
    17161 aagcaaaact taaagaacaa gtgggtcaag cgacaacgtt gccaaatgtt caaactgttc
    17221 gtgataatgc acaaacatta aacactgcaa tgaaaggtct acgagatagc attgcgaatg
    17281 aggcgacgat taaagcagaa caaaactaca cggatgcaag tcctaataat cgtagtgaat
    17341 acgatagtgc agtcactgca gcaaaagcaa tcattggtca aacatcaagt ccaacaatga
    17401 atgctcaaga aatcaatcaa gcgaaagacc aagtgacagc taaacaacaa gcgttaaacg
    17461 gtcaagaaaa cttaacaaac gcacaaataa atgcgaaaca acatttgaat ggcttaagtg
    17521 acttaactaa tgcacaaaaa gatgcagcaa aacgccaaat cgaaggtgca acgcatgtta
    17581 gtgaagtaac acaagcgcaa aataatgcgg acgcattaaa tacagctatg acgaacttga
    17641 aaaatggtat tcaagatcaa aatacgatta agcaaggtgt taacttcact gatgcagatg
    17701 aagcgaaacg taatgcatat acaaatgcag tgacgcaagc tgaacaaatt ttaaataaag
    17761 tacaaggtcc aaatactgca aaagacaatg ttgaatctgc gttacaaaat gtacaacgtg
    17821 ctaaaaacga tttgaacggt aatcaaaatg ttgcaaatgc taagacaact gcgaaaaatg
    17881 cattaaataa tttaacatca attaataatg cacaaaacga agcgttgaaa tcacaaattg
    17941 atagtgcgac aacagttgca ggtgtaaatc aagtgtctgc aacggcatct gaattaaata
    18001 cagcaatgag caacttacaa aatggtatta atgatgaagc agctacaaaa gcagctcaga
    18061 aatatactga tgcagatagt gataaacaaa ctgcatacaa tgatgctgta acagcagcta
    18121 aaacgttatt agataaaaca gctggcacaa atgacaataa agcagccgtt gaacaagcat
    18181 tacaacgtgt gaatactgct aaaactgcat taaatggtga cgcgcgatta aatcaagcga
    18241 agaacacagc taaacaacaa ttagcgacaa tgtcacattt aactgatgct caaaaagcaa
    18301 acttaacatc gcaaatcgaa cgcggtacga ctgttgcagg tgttcaaggt attcaagcta
    18361 atgccggtac tttaaatgaa gcaatgaatc aattgagaca aagcattgct tctaaagatg
    18421 cgactaaagc gagcgaagat tatcatgatg cgaatacaga tttacaaaat gcatacaatg
    18481 atgcggtaac taatgctgaa ggtattatta gtgcaacgaa taaccctgaa atgaatccag
    18541 atacgattaa tcaaaaagcg agccaagtga acagtgcgaa gtctgcattg aacggtgatg
    18601 aaaaattagc agcagcaaaa caaactgcga aatcagacat cggtcgttta acagacttga
    18661 acaatgcaca acgaactgcg gcaaatgctg aagtggatca agcaccaaat cttgcagctg
    18721 tcacagcggc taaaaataaa gcaacatcgt taaatacagc gatgggtaat ttgaaacatg
    18781 cacttgctga aaaggataat acgaaacgta gtgtcaatta cacagatgcg gatcaaccta
    18841 aacaacaagc gtatgatact gcggttacac aagcagaagc aattactaat gctaatggca
    18901 gcaacgcgaa tgaaacacaa gttcaagcag cgcttaacca attgaatcaa gctaaaaatg
    18961 acttgaatgg tgataataaa gttgctcaag ctaaagaaac tgcgaaacgt gcattagctt
    19021 catatagtaa cttgaataat gcgcaatcaa ctgcagcaac tagtcaaatt gacaatgcaa
    19081 cgacagtagc aggcgtaact gctgcacaaa atactgctaa tgagttaaat acagcaatgg
    19141 gtcaacttca aaatggtatt aatgaccaaa acactgttaa acaacaagtg aactttacag
    19201 atgctgacca aggtaagaaa gatgcttaca caaatgctgt tacgaatgct caaggtattt
    19261 tagataaagc acacggtcaa aatatgacga aagcacaagt tgaagctgca ttaaatcaag
    19321 taacgactgc taagaatgct ttaaacggtg acgcaaatgt aagacaagca aaatcagatg
    19381 cgaaagcaaa cttaggcaca ttaacacact taaataatgc acaaaaacaa gatttaacat
    19441 cacaaatcga aggcgcaaca acagtcaacg gtgtaaatgg tgttaaaacg aaagcacaag
    19501 acttagatgg tgcaatgcaa cgattacaat cagcaatcgc aaataaagat caaactaaag
    19561 cgagcgaaaa ttacatcgac gcagatccaa ctaacaaaac agcatttgat aatgccatca
    19621 cacaagcaga atcatactta aataaagatc acggtgcgaa taaagataag caagctgttg
    19681 aacaagcaat tcaaagtgta acgtctactg aaaatgcttt gaacggtgac gcaaacttac
    19741 aacgtgctaa aactgaagct acgcaagcta tcgataactt gacacatttg aatacaccac
    19801 aaaaaacagc attaaaacaa caagtgaatg ctgcgcaacg tgtatcaggt gtaactgatc
    19861 tgaaaaatag tgctacatca cttaataatg cgatggatca attaaaacaa gcaattgctg
    19921 atcatgacac aattgtagct ggtggtaatt acactaacgc gagtcctgat aagcaaggtg
    19981 cttatactga tgcatataat gctgcgaaaa acattgtaaa tggttcacct aatgtgatta
    20041 caaatgcagc agatgttaca gcagcaacac aacgtgtcaa taatgctgaa acaggtttaa
    20101 acggtgatac aaacttagca actgcgaagc aacaggctaa agatgcatta cgtcaaatga
    20161 cacatttatc tgatgcacaa aaacaaagta ttactgttca aattgataat gcgacacaag
    20221 taactggcgt tcaaagtgtg aaagacaacg cgacaaatct tgataatgca atgaatcaac
    20281 ttcgaaatag tattgcgaat aaagatgaag taaaagcgag tcaaccatat gttgatgcag
    20341 atagagataa acaaaatgca tacaacacag ctgttacaag tgctgaaaat atcattaatg
    20401 caacgagtca gccgacactt gatccatctg cagtaacaca agcagctaat caagtgaaca
    20461 ctaacaaaac tgcgcttaat ggtgcacaaa acttagcaaa taaaaagcaa gaaacaactg
    20521 ccaacatcaa ccaattaagt catttaaata atgctcaaaa gcaagattta aatacgcaag
    20581 tgacaaatgc accaaatatt agcacagtaa gtcaagtgaa aactaaagct gaacaattag
    20641 atcaagcaat ggaacgttta atcaacggaa tccaagacaa agatcaagtg aaacaaagtg
    20701 ttaactttac agatgcagat ccagaaaaac aaacagcata caacaatgcg gtaactgctg
    20761 ctgaaaatat tattaatcaa gcaaatggta caaatgcgaa ccaatcacag gttgaagcag
    20821 cactttcaac tgtaacaact actaaacaag cattgaatgg tgatagaaaa gtaacagatg
    20881 ctaaaaacaa tgcaaaccaa acattatcta cgttagataa cttaaacaat gcacaaaaag
    20941 gtgctgttac tggaaacatc aatcaagcgc acactgttgc tgaagtaacg caagccattc
    21001 aaacagctca ggaactaaat acagcgatgg gtaacttgaa aaatagcttg aatgataaag
    21061 acactacact tggcagtcaa aactttgcag atgcagatcc agagaagaaa aatgcataca
    21121 atgaagcggt tcgtaatgct gaaaatattt taaataaatc tacaggtaca aacgtgtcta
    21181 aagatcaagt tgaagcagct atgaatcaag tgaatactac aaaagcagcg cttaatggta
    21241 ctcaaaacct tgaaaaagct aaacaacacg caaatacagc gattgacggt ttaagccatt
    21301 taacaaatgc acaaaaagag gcattaaaac aattggtaca acaatcgact actgttgcag
    21361 aagcacaagg taatgaacaa aaagcaaaca atgttgatgc agcaatggac aaattacgtc
    21421 aaagtattgc agataatgcg acaacaaaac aaaaccaaaa ttatactgat tcaagtccga
    21481 ataaaaagga tgcgtacaat aatgctgtca caactgcaca aggtattatt gatcaaacga
    21541 caagtccaac tttagatccg actgttatca atcaagctgc tggacaagta agcacaacta
    21601 aaaatgcatt aaatggtaat gaaaacctag aggcagcgaa acaacaagcg acacaatcat
    21661 taggttcatt agacaactta aataatgcgc aaaaacaagc agttactgat caaattaatg
    21721 gcgcgcatac tgttgatgaa gcaaatcaaa ttaaacaaaa tgcgcaaaac ttaaatacag
    21781 cgatgggtaa cttgaaacaa gcgattgctg acaaagatgc tacgaaagca acagtaaact
    21841 tcactgatgc agatcaagca aaacaacaag catataacac tgctgttaca aatgctgaaa
    21901 acatcatttc aaaagctaat ggtggcaatg caacacaaac tgaagttgaa caagcaatcc
    21961 aacaagttaa tgctgcaaaa caagcattaa atggtaatgc caacgttcaa catgcgaaag
    22021 acgaagcaac agcattaatt aatagctcta atgaccttaa ccaagcacaa aaagacgcat
    22081 taaaacagca agttcaaaat gcaactactg tagctggtgt aaacaatgtt aaacaaacag
    22141 cgcaagagtt aaacaatgca atgacacaat taaaacaagg cattgcagat aaagaacaaa
    22201 caaaagctga tggtaacttt gtcaatgcag atcctgacaa acaaaatgca tataaacaag
    22261 cagtagcaaa agctgaagca ttaattagcg gcacacctga tgttgtcgtt acacctagcg
    22321 aaattactgc agcgttaaat aaagttacac aagctaaaaa tgatttaaat ggtaatacga
    22381 acttagcaac aacgaaacaa aatgttcaac atgctattga tcaattgcca aacttgaatc
    22441 aagcgcaacg tgatgaatac agcaaacaaa ttacgcaagc aacacttgta ccaaacgtca
    22501 atgctattca acaagcggca actacgctta atgatgcgat gacacaattg aaacaaggta
    22561 ttgcgaataa agcacaaatt aaaggtagcg agaactatca cgatgctgat actgacaagc
    22621 aaacagcata tgataatgca gtaacaaaag cagaagaatt gttaaaacaa acaacaaatc
    22681 caacaatgga tccaaataca attcaacaag cattaactaa agtgaatgat acaaatcaag
    22741 cacttaacgg taatcaaaaa ttagctgatg ctaaacaagc tgctaagaca aatcttggca
    22801 cattagatca tttaaatgat gcgcaaaaac aagcgttaac aacacaagtt gaacaagcac
    22861 cagatattgc aacagttaat aatgttaagc aaaatgctca aaatctgaat aatgctatga
    22921 ctaacttaag taatgcatta caagataaaa ctgagacatt aaatagcatt aactttactg
    22981 atgcagatca agctaagaaa gatgcttata ctaatgcggt tgcacatgca gaaggtattt
    23041 tatctaaagc aaatggcagc aatgcaagtc aaaccgaagt ggaacaagcg atgcaacgcg
    23101 tgaacgaagc gaaacaagca ttgaatggta atgacaatgt acaacgtgca aaagatgcag
    23161 cgaaacaagt gattacaaat gcgaatgatt taaatcaagc gcaaaaagat gcgttaaaac
    23221 aacaagtcga tgctgcgcaa actgttgcaa atgtaaacac gattaagcaa acagcacaag
    23281 atttaaatca agcaatgaca caattgaaac aaggtattgc agataaagac caaactaaag
    23341 caaatggtaa ctttgtcaat gctgatactg ataagcaaaa tgcatacaac aatgcggtag
    23401 cacatgctga acaaatcatt agtggtacac caaatgcaaa cgtggatcca caacaagtga
    23461 ctcaagctaa gggtgattta aacggtaacc ataacttaca agttgctaaa gacaatgcga
    23521 atactgccat tgatcagtta ccaaacttaa atcaaacaca aaaaacagca ttaaaagacc
    23581 aagtgtcaca tgcagaactt gttacaggtg ttaatgctat taagcaaaat gctgatgcat
    23641 taaataatgc aatgggtacg ttgaaacaac aaattcaagc aaacagtcaa gtaccacaat
    23701 cagttgactt tacacaagcg gatcaagaca aacaacaagc atataacaat gcagctaacc
    23761 aagcgcaaca aattgcaaat ggcacaccaa cacctgtatt gacgcctgac gcagtaacac
    23821 aagcagttac tacaatgaat caagcgaaag atgcattaaa cggtgatgaa aaattagcgc
    23881 aagcgaaaca agatgcaata gcaaatctag atacgttgcg tgacttaaat caaccacaac
    23941 gtgatgcgtt acgtaatcaa atcaatcaag cacaagcgtt agctacagtt gaacaaacta
    24001 aacaaaatgc acaaaatgtt aatacagcaa tgagtaactt gaaacaaggt attgcaaata
    24061 aagataccgt caaagcaagt gaaaactatc acgatgctga tgccgataag caaacaacat
    24121 atacaaatgc agtgtctcaa gcggaaggta ttatcaatca aacgacaaat ccaatgctta
    24181 acccagatga tattactcgt gcattaactc aagtgactga tgctaaaaat ggcttaaacg
    24241 gtgaagctaa attggcaact gaaaagcaaa atgctaaaga tgctgtcaat gcaatgacgc
    24301 atttgaacga tgctcaaaaa caagcattaa aaggacaaat cgatcaatcg cctgaaattg
    24361 ctatagtgaa ccaagttaaa caaacagcaa cgagcctaga tcatgcaatg gatcaattat
    24421 cacaagctat taatgataaa gctcaaacat tagcggacgg taattactta aatgcagatc
    24481 ctgacaaaca aaatgcgtat aaacaggcag tagcaaaagc tgaagcatta ttgaataaac
    24541 aaagtggtac taatgaagta caagcacaag ttgaaagcat cactaatgaa gtgaacgcag
    24601 cgaaacaagc attaaatggt aatgataatt tagcgaatgc taagcaacaa gcgaaacaac
    24661 aactggcgaa tctaacacac ttaaatgatg cacaaaaaca atcatttgaa agtcaaatta
    24721 cacaagcgcc acttgttaca gatgtcacta caattaatca aaaagcacaa gcgttagatc
    24781 atgcgatgga attattaaga aatagtgttg cggataatca agcgacatta gcgtctgaag
    24841 attatcatga tgcaactgcg caaagacaaa atgactataa ccaagcagta acagctacta
    24901 ataatatcat taaccaaaca acatcaccta caatgaatcc agatgaagtt aatcgtgcaa
    24961 cgacacaagt gaataacacg aaagttgcat tagatggtga tgaaaacctt gtagcagcta
    25021 agcaacaagc gaacaataga cttgatcaat tagatcattt aaataatgcg caaaagcaac
    25081 agttacaatc acaaattacg caatcatctg atattgctgc agttaatggt cacaaacaaa
    25141 cagcagaatc tttaaataca gcaatgggta acttaattaa tgcgattgca gatcatcaaa
    25201 ccgttgaaca acttggtaac ttcgtcaatg ctgatactga taaacaaact gcttatacta
    25261 cagcggtaaa tgaagcagaa gccatgatta acaaacaaac tggtcaaaat gcgaaccaaa
    25321 cagaagtaga acaagctatt actaaagttc aaacaacact tcaagcgtta aatggagatc
    25381 ataatttaca agttgctaaa gcaaatgcga cgcaagcaat tgatgcttta acaagcttaa
    25441 atgatcctca aaaaacagca ttaaaagacc aagttactgc tgcaacttta gtaactgcag
    25501 ttcatcaaat tgaacaaaat gcgaatacgc ttaaccaagc aatgcatggc ttaagagaaa
    25561 gtattcaaga taacgcagca actaaagcaa atagcaaata tatcaacgaa gatcaatcag
    25621 aacaacaaaa ctacgatcaa gcagtgcaag ccgcaaataa tattatcaat gaacaaacag
    25681 caacattaga taataatgca attaatcaag cagcgacaac tgtgaataca acgaaagcag
    25741 cattacatgg tgatgtgaaa ttacaaaatg ataaagatca tgcgaaacaa acagttagtc
    25801 aattaacaca tttaaacaat gctcaaaaac atatggaaga tacgttgatt gatagtgaaa
    25861 caactagaac agccgttaac catgatttga ctgaagcaca agcattagat caacttatgg
    25921 atgcattaca acaaagtatt gctgacaaag atgcaacacg tgcgagcagt gcatatgtca
    25981 atgcagaacc gaataaaaaa caatcatatg atgaagcagt tcaaaatgct gagtctatca
    26041 ttgcgggatt aaataatccg actatcaata aaggtaatgt aacaagtgcg acacaagctg
    26101 taacatcatc taaaaatgca ttagacggtg ttgaacgatt agcgcaagat aagcaaactg
    26161 ctggaaattc tctaaatcat ttagaccaat taacaccagc acaacaacaa gcactagaaa
    26221 atcaaattaa taatgcaaca actcgtgata aagtggctga aattattgca caagcgcaag
    26281 cattaaatga agcgatgaaa gcattaaaag aaagtattaa agatcaacca caaactgaag
    26341 caagtagtaa atttattaat gaagatcaag cacaaaaaga tgcttatacg caagctgtac
    26401 aacacgcgaa agatttgatt aacaaaacaa ctgatcctac attagctaaa tcaatcattg
    26461 atcaagcgac acaggcagtg actgatgcta aaaacaattt acatggtgat caaaaactag
    26521 atcaagataa gcaacgtgca acagaaacgt taaataactt gtctaacttg aatacaccac
    26581 aacgtcaagc acttgaaaat caaatcaata atgcagcaac tcgtggcgaa gtagcacaaa
    26641 aattaactga agcagaagca cttaaccaag caatggaagc tttacgtaat agcattcaag
    26701 atcaacagca aacggaagcg ggtagcaagt ttatcaatga agataaacca caaaaagatg
    26761 cttaccaagc agcagttcaa aatgcaaaag atttaattaa tcaaactaac aatccaacgc
    26821 ttgataaagc acaagttgaa caattgacac aagctgttaa ccaagctaaa gataacctac
    26881 acggtgatca aaaacttgca gacgataaac aacatgcggt tactgattta aatcaattaa
    26941 atggtttgaa taatccgcaa cgtcaagcac ttgaaagcca aataaacaac gcagcaactc
    27001 gtgatgaagt agcgcaaaaa ttagctgaag caaaagcgct tgatcaagca atgcaagcat
    27061 tacgtaatag tattcaagat caacaacaaa cagaatctgg tagcaagttt atcaatgaag
    27121 ataaaccgca aaaagatgct taccaagcag cagttcaaaa tgcaaaagat ttaattaacc
    27181 aaacaggtaa tccaacactc gacaaatcac aagtagaaca attgacacaa gcagtaacaa
    27241 ctgcaaaaga taacctacat ggtgatcaaa aacttgctcg tgatcaacta caagcagtaa
    27301 caactgtaaa tgcattgcca aacttaaatc atgcacaaca acaagcatta actgatgcta
    27361 taaatgcagc gcctacaaga acagaggttg cacaacatgt tcaaactgct actgaacttg
    27421 atcacgcgat ggaaacattg aaaaataaag ttgatcaagt gaatacagat aaggctcaac
    27481 caaattacac tgaagcgtca actgataaaa aagaagcagt agatcaagcg ttacaagctg
    27541 cagaaagcat tacagatcca actaatggtt caaatgcgaa taaagacgct gtagaccaag
    27601 cattaactaa gcttcaagaa aaagtgaatg agttaaatgg taatgagaga gtcgctgaag
    27661 ctaaaacaca agcgaaacaa actattgacc aattaacaca tttaaatgct gatcaaattg
    27721 caactgctaa acaaaatatt gatcaagcga cgaaacttca accaatcgct gaattagtag
    27781 atcaagcaac gcaattgaac caatcaatgg atcaattaca acaagcagtt aatgaacatg
    27841 ctaacgttga gcaaactgta gattacacac aagcagattt agataagcaa aatgcttata
    27901 aacaagctat tgctgatgcc gaaaatgtat tgaaacaaaa tgcgaataag caacaagtgg
    27961 atcaagcact tcaaaatatt ttaaatgcaa aacaagcatt aaatggtgat gaacgtgtag
    28021 cacttgctaa aacaaatggt aaacatgata tagagcaatt gaatgcatta aacaatgctc
    28081 aacaagatgg atttaaaggt cgcatcgatc aatcaaacga tttaaatcaa atccaacaaa
    28141 ttgtagatga ggctaaggca cttaatcgtg taatggatca attgtcacaa ggaatcactg
    28201 gcaatgaagg acgcacgaaa ggtagcacga actatgtcaa tgcagataca caagttaaac
    28261 aagtgtatga tgaagcggtt gataaagcga aacaagcact tgataaatcg acaggtcaaa
    28321 acttaactgc agaacaagtt atcaaattaa atgatgcagt cactgcagca aagaaagcat
    28381 taaatggtga agaaaaactt aataatcgta agtctgaagc attacaaaga ttggatcaat
    28441 taacacatct aaacaatgct caaagacaat tagcaatcca acaaattaat aatgctgaaa
    28501 cgctaaataa agcatctcga gcaattaata gagcaactaa attagataat gcaatgggtg
    28561 cagtacaaca atatattgac gaacagcacc ttggtgttat cagcagcaca aattacatca
    28621 atgcagatga taatttgaaa gcaaattatg ataatgcaat tgcgaatgca gcacatgagt
    28681 tagataaagt gcaaggtaat gcaattgcaa aagctgaagc agagcaattg aaacaaaata
    28741 ttattgatgc tcaaaatgca ttaaatggag accaaaacct tgcaaatgcc aaagataaag
    28801 caaatgcgtt tgttaattcg ttaaatggat taaatcaaca gcaacaacat cttgcacata
    28861 atgcaattaa caatgccgat actgtatcag atgtaacaga tattgttaat aatcaaattg
    28921 acttaaatga tgcaatggaa acattgaaac atttagttga caatgaaatt ccaaatgcag
    28981 agcaaactgt caattaccaa aacgctgaag ataatgctaa aacaaacttc gatgatgcta
    29041 aacgtctagc aaatacattg ctaaatagtg ataacacaaa tgtgaatgat attaacggcg
    29101 caattcaaac agtcaatgat gcaatccaaa atcttaatgg tgatcaacga ctacaagatg
    29161 ctaaagacaa ggcaattcaa tctattaatc aagctttagc taataagcta aaagaaatcg
    29221 aagcttcaaa tgcgacggat caagacaagc ttattgcgaa aaataaagca gaagaattgg
    29281 caaacagcat catcaacaac attaataaag caacaagtaa tcaggatgta tctcaagttc
    29341 aaacagctgg caaccatgcg attgaacaag tgcatgccaa tgaaataccg aaagctaaaa
    29401 ttgatgcgaa taaagatgtt gacaagcaag ttcaagcatt aattgacgaa attgatcgaa
    29461 atccaattct aacagataag gaaaaacaag cgcttaaaga tcgaattaac caaatacttc
    29521 aacaaggtca taacgacatt aacaatgcga tgactaaaga agaaattgaa caagccaaag
    29581 cacaacttgg acaagcgtta caagacatta aagatttagt gaaagctaaa gaaagcgcga
    29641 aacaagatat tgataagcaa gttcaagcat taattgacga aattgatcga aatccaattc
    29701 taacagataa ggaaaaacaa gcgcttaaag atcgaataaa tcaaatcctg caacaaggtc
    29761 ataacggcat taacaatgcg atgactaaag aagaaattga acaagccaaa gcacaacttg
    29821 gacaagcgtt acaagacatt aaagatttag tgaaagctaa agaaagcgcg aaacaagata
    29881 ttgataagca agttcaagca ttaattgacg aaattgatcg aaatccaaat ctaacagata
    29941 aggaaaaaca agcgcttaaa gatcgaataa atcaaatctt gcaacaaggt cataacggca
    30001 ttaacaatgc gatgactaaa gaagaaattg agcatgccaa agcgcagctt gcacaagcat
    30061 tacaagacat taaagattta gtgaaagcta aagaagatgc gaaaaatgca attaaagcat
    30121 tagcaaatgc gaagcgtgat caaatcaatt caaaccctga tttaacactt gaacaaaaag
    30181 caaaagcgct caaagaaatt gacgaagctg aaaaacgagc actagaaaac attgaaaatg
    30241 ctcaaactaa agatcaatta aatcaaggat taaatttagg attagatgat attagaaata
    30301 cacatgtatg ggaagttgat gcacaacctg ctgtaaatga aatttttgac gcaactcctg
    30361 aacaaatcct tgtaaatggc gaactcattg tccatcgtga tgacatcatt acagaacaag
    30421 atattcttgc acacatcaac ttaattgatc aacttacagc ggaaattatt gatacaccgt
    30481 caactgcaac aatttctgat agtttaacag caaaagttga agtaacattg cttgatggat
    30541 caaaagtgat tgttaatgtt cctgtgaaag tagtagaaaa agaattgaca gtagttaaac
    30601 agcaggcgat tgaatcaatt gaaaatgcgg cacaacaaaa gattaatgaa atcaataatc
    30661 acgcaacatt aacacctgaa caaaaagaag ctgcgattgc ggaagttaat aagcttaaac
    30721 aacaagctat tgagcaaatt aacaacgcag cagatgttca tacggtggaa gaagttcaac
    30781 accaagaaca ggcgcatatt gaacaattca atccagatca atttacaatt gaccaagcca
    30841 aatcaaatgc cattaaatca atttctgatg ctatccaaca tatgattgat gaaattaatg
    30901 ctagtaagga tttaactgat aaagagaagc aagaagctat atctaaatta aatcagttaa
    30961 aagatcaatc aattcaggcg attcaacgtg cgcaaagcat cgatgaaatt gcgcaacaat
    31021 tagagcaatt taaagcgcaa ctaaaagcgg ccaatccatt tgctaaagaa ttggaaaacc
    31081 gtaaaaaagc tgcgatttca aaaattaaag atatttctac agataaaatt gatcgcatta
    31141 gaaatagcac aattggaact gctgaagaaa gacaagcagc aatgaatcga attaacgaaa
    31201 ttgtgcttga aacaatcaaa gatattaata atgcgcaaac gccacaacaa gttgaggctg
    31261 cattgaataa tggtatcgca cgaattttag cagtacaaat tgtaacatca gatcatagca
    31321 aaccttcatc aaattcagat ggtcaatcta atagtcattt acacgttggt tatggcactg
    31381 taaatcatcc attcaatagt tcgcctattg gacataagaa gaaattggat caagatgatg
    31441 aaatcgatcc acttcatatg cgtcatttcg gtgaccgaat tggtaatgtt atcaaaaacg
    31501 cacttggcgt tgttggtatt tctggattac ttgctagctt ctggttcttc attgcgaaac
    31561 gtcgtcgtaa agaggatgaa gaggaagaat tggaaattag agataatagc aaagacaaga
    31621 agaaaggttc aatagaaggt actaaacatt taccactttt atttgcgaaa cgtcgtagaa
    31681 aagaagatga agaagatgct attgttgaag aaaaagattc gctaaacaat gacgagtcac
    31741 tcgataaagt taaacatacg ccgttcttct tacctaaacg tcgcagaaaa gaagatgaag
    31801 aagatgtgga agttacaaat gaaaacacag atgaaaaagt gttgcaagat aacgaacatt
    31861 caccggtctt aatcgcaaaa cgactcaaag ataaagatgg aaatgttgaa acaacaacta
    31921 gtattgaatc taaagatgaa gacgttcctt tattattggc taaaaagaaa aatcaaaaag
    31981 ataaccaatc caaagggaaa aagtcggcat caaaaaaacc ttctaaaaag gtagcagcta
    32041 aaaagaagaa aaagaaatct aaaaaaaata aaaaataatt tgtttcttcg ataaaaagag
    32101 gagcaccgat tgaaaatata tcagtcggtg ctccttttat ttgttctttt taataaattt
    32161 gtacaatgct tgctgtgcat gttgattcgc ttctttgttt tgttctctcg gaatccattt
    32221 aacaaataat aaatcaaaat cttgttcaaa tatttccaat tgatcaaaat aaggtttgaa
    32281 tttggcattt tttacataac cagcgttaac actatccgca attaacttag agtcagtgta
    32341 caacaacgca ttactaacat ttaattcacg tgcatgttct agtgcataaa tacatgctgc
    32401 ccattctgct gtgtgattat ccatttcacc taactcatgt gtatatgtat aatgctgctc
    32461 atcttctttg attacaatgg cacaagtact tatgcctgga tttcctttcg tcgcagcatc
    32521 aaaatttatt ttcgccataa taaacctact ttctattcaa tacttagtta aagttactat
    32581 tactgtaata caaaatacgt tgggtaatcc ataaaaaaac acgcatcact taaataagta
    32641 acacgtgttt aaaatactcg ctgagtcaaa gatgattttc taatacgtag tactgtaata
    32701 tacttcctaa aaaaatcatc ttgataaaat tttctcaaat atcagcctta aaacttattc
    32761 ttttatgtag taagtgcttt aatagccttg atctaattta tcaatcttac ctttacgata
    32821 aaatgattta gcaatatatc caaatggtac attgaaaact gttgaagcta attttagtac
    32881 gtacgttgta ataaatattt caaatacaac tgtaccaggt aaacttccga taaatgcgat
    32941 agctacaaat atagctgtat caattattga gcttaaaaat gtactaccgt atgcacggat
    33001 gaaaaacgtt ttatcagaac taaatacttt tttaattagt gaaaagataa atacatcaat
    33061 atgttgacca ataatatatg cgacaattga gcctaaagct atacgtggca caacatcaaa
    33121 gattgcgtgt aatgcttttt gtgccatatc ttctggtgca ggaataaaat gtaatgacaa
    33181 ttgcataaca ataatcataa ttaatgttga tgaaaagcct aaccaaactg ctctttttgc
    33241 aactctacgc ccataaatat cgtttaatat atctgttgct aaataaatag aagcaaacat
    33301 gacattacct aaagttgctg aaataccaaa gatttctaca gttttaatca cttgtatgtt
    33361 ggcaatgatt gtgccaattg caacccatgc aattaaacct tgtttaccaa aaaagcgata
    33421 cataagtacc ataagcacga acgttgcaat aaacgtaact agtcctaaaa tttcattata
    33481 catattaaaa tgtcctccta aattttgatc atacgggtgt ttagaaaccg ctcaataaat
    33541 aacttactca ttttactgaa aaatcgtttg cgatgcaata gcatttgaat ataaatacat
    33601 tttacgtata gaattgtaaa aggtttcatt caaatcttag ggtcaaaaat gttataatat
    33661 ttttatgtca aatttaaaac agtaacactt atttacaagg ttgcaatatt ttgaagtaat
    33721 aaaggaagtg tcgcgtattt taacttattc agagcaaaat gcactcgcga aaatagatga
    33781 tttaatgaat acttattgca atcaatgtcc aatcaaaact cgtctgcgta aattagaggg
    33841 gaaaacgaag gcgcatcatt tttgtatcaa tgagtgttca atagggaaag aaataaaaca
    33901 attaggaaat gaacttcaat aggaggaagt caaatgcaaa ttatatctat atcagaaaca
    33961 ccgaaccaca acacaatgaa gattacactt agtgaaagca gagaaggtat gacatcagat
    34021 acgtatacta aagttgatga ttcacagcca gcatttatta atgacatctt aaaggttgaa
    34081 ggcgttaaat caattttcca tgttatggac tttatttcag tagataaaga aaatgacgca
    34141 aattgggaaa cagtattgcc aaaagtagag gctgtattcg aataaatttt tcatcaacta
    34201 gtattcgggg ggaataaagt atatggaaat tttacgtata gagccaacac ctagtccaaa
    34261 tacaatgaaa gttgttttgt catatacaag agaagacaag ttatctaata cttataaaaa
    34321 agtagaagaa aatcaaccaa gatttataaa tcagttgtta tctatagatg gtatcacttc
    34381 catttttcat gtcatgaact tcttagctgt tgataaggca ccaaaagctg attgggaaga
    34441 catactacct gatattaaag ctgctttttc aggtgaaagt caggtgttgg aatctggaaa
    34501 agacccacaa attgacaatc attttggtga aattaaagct gaactattaa cttttaaggg
    34561 tataccgtat caaattaagc taacttctgc tgaccaagaa ttaagagaac aattaccaca
    34621 aacatatgtt gatcatatga ctcaagcgca aacaaaacat gacaatattg tttttatgcg
    34681 taaatggcta gatttaggaa atcgctatgg aaatattgaa gaagtaatgg atggtgtcct
    34741 agaagaagta ctagctacct atccagaatc acagttacct gtattggtaa aacatgcttt
    34801 agaagaaaat cacgcaacta ataattatca tttctatcga catgtctctt tggatgaata
    34861 tcatgcgact gataattgga agactcgatt aagaatgtta aatcattttc caaagccgac
    34921 ttttgaagat ataccgctgc ttgatttagc tttatctgat gaaaaagtac cggttagacg
    34981 tcaagcgatt gtactattag gtatgattga aagtaaagaa attttaccgt atttatataa
    35041 ggggcttcgt gataaaagtc ctgctgtaag aagaactgca ggggattgca taagcgattt
    35101 agggtatcca gaggcactac cagaaatggt gctactatta gatgatccac agaaaatcgt
    35161 taggtggcgt gctgctatgt ttatctttga tgaaggtaat gcagagcagc ttcccgcact
    35221 taaagcccat attaatgaca atgcgtttga agttaaatta caaattgaaa tggccatatc
    35281 tcgtattgaa aacggagatg aagcacttgg ttcagtatgg aaacaaatga cgaacagaac
    35341 aatttaattg gaggaattaa atatgaatgc atatgatgct tatatgaaag aaattgcgca
    35401 acaaatgcgt ggcgaattaa ctcaaaatgg ttttacaagt ttagaaacga gcgaggaagt
    35461 atcagagtat atgaaccaag taaatgctga tgacactact tttgtagtta ttaactctac
    35521 atgcggctgt gcagctggat tagcaagacc agctgcagta gctgttgcaa cacaaaatga
    35581 acatagacct acaaatacag ttacagtttt tgctgggcaa gataaagaag caactgctac
    35641 aatgcgagaa ttcattcagc aggtaccatc tagtccttcg tatgctttat tcaaaggtcg
    35701 agatttagtt tattttatgc ctagagaatt tatcgaaggt agagatatta atgacattgc
    35761 aatggactta aaggatgcct ttgacgaaaa ttgtaaatag tacacataaa taaatataaa
    35821 ggttaacaca ttttataata ttaaaaatgg tgtctgtcat tgaaaataga gaatatagtt
    35881 gtattccatt tgttatataa agaacgttct taccaactat attttctaga aatttaactg
    35941 ttttaatagg acatcaaaca taatattcaa atcatgtgtt aacctctttt ttaaaatttt
    36001 ttagcattaa agttatagat ttgggtaaac aattagcaat tggaaacata gatcacgtta
    36061 tgatgtggca ggtacttaat catcatttta taaataaagt aacggaattc atgatattaa
    36121 tatcatattc ctaaaatgag tgataacaaa atgctacata aagttaaatt atatcgaact
    36181 aaatatacat actataaata ataaaaatga ggtgttatcg catatgttga attcatttga
    36241 tgcagcatat cacagtcttt gtgaagaagt tttagaaata ggaaatacac gaaatgatcg
    36301 cacaaataca ggtacgattt cgaaatttgg tcatcaactt cgctttgact tatctaaagg
    36361 atttccacta ttaacgacaa agaaagtttc ttttaaatta gtagcgaccg aattattatg
    36421 gttcattaaa ggagatacaa atatccaata cttattaaag tataataata atatttggaa
    36481 cgaatgggct tttgaaaatt atatcaaatc agacgagtat aaaggtccag atatgacaga
    36541 tttcgggcat cgtgcattga gtgatcctga atttaacgaa caatataaag aacaaatgaa
    36601 acaatttaag caacgtattc ttgaagatga tacatttgcg aagcaattcg gtgatttagg
    36661 aaatgtttat ggtaaacagt ggcgagattg ggttgataaa gatggtaatc attttgatca
    36721 acttaaaaca gtaattgagc aaattaagca taatccagat tcaagacgac acatcgtatc
    36781 tgcatggaat ccaacagaaa ttgatacaat ggcacttccg ccttgtcata caatgttcca
    36841 gttttatgtt caagatggta agttaagttg ccagttatac caacgtagcg cagatatctt
    36901 tttaggtgtg ccatttaata tcgcaagcta cgctttattg acgcacctta ttgccaaaga
    36961 atgtggactt gaagtaggag aatttgtgca tacatttgga gatgcacata tttattcaaa
    37021 tcatattgat gcgattcaaa cacaattagc acgtgaaagc ttcaatcctc caacattaaa
    37081 aattaacagt gataagtcta tttttgacat aaattatgaa gatttggaaa ttgttgacta
    37141 tgaatcacat ccagcaataa aagctccaat agcagtgtag tcgttgcata gttagctaac
    37201 catatagaca tcaaaatgac atcaaagtat tttcaagtgc aaaaaagtac ttttttgtgt
    37261 taaacgtttt cataaattat gcaaaatcat tatttctatc acactttatg ataaaaattg
    37321 tgttaaatta aagataactt agtaattaaa aatgaaatga tagaagaagg aggataatca
    37381 tgactttatc cattctagtt gcacatgact tgcaacgagt aattggtttt gaaaatcaat
    37441 taccttggca cctaccaaat gatttgaagc atgttaaaaa attatcaaca ggtcatactt
    37501 tagtaatggg tcgtaagaca tttgaatcga ttggtaaacc actaccgaat cgtcgaaatg
    37561 ttgtacttac ttcagataca agtttcaacg tagagggcgt tgatgtaatt cattctattg
    37621 aagatattta ccaactaccg ggccatgttt ttatatttgg agggcaaaca ttatttgaag
    37681 aaatgattga taaagtggac gacatgtata ttactgttat tgaaggtaaa tttcgtggtg
    37741 atacgttctt tccaccttat acatttgaag actgggaagt tgcctcttca gttgaaggta
    37801 aactagatga gaaaaataca attccacata cctttctaca tttaattcgt aaaaaataag
    37861 ggggaaaacg accatgacaa aacagattat agtaacagac tcaacatccg atttatctaa
    37921 agaatattta gaagcaaaca acatacatgt aattccttta agtttaacta ttgaaggagc
    37981 ttcatacgtt gaccaagtag atattacatc agaagaattt attaatcata ttgaaaatga
    38041 tgaagatgta aagacaagtc agccagccat aggtgaattt atatctgctt atgaagaact
    38101 aggaaaagat ggttctgaaa tcataagtat tcatctttct tcaggactaa gtggtacatt
    38161 taacactgct taccaagcaa gtcaaatggt agatgctaat gtaactgtta ttgattcaaa
    38221 atctatttct tttggtttag ggtatcaaat acaacaccta gtagagcttg taaaagaagg
    38281 tgtctcaact tctgaaatag ttaaaaagtt aaatcattta agagaaaaca ttaaattatt
    38341 tgtagttata gggcaattga atcaattaat taaaggtggc agaattagta aaacaaaagg
    38401 tttgattggt aatcttatga aaattaaacc aattggtaca ctagatgatg gtcgcttaga
    38461 gcttgtgcac aatgcgagaa ctcaaaattc tagtatccaa tacttgaaaa aggaaattgc
    38521 tgaatttata ggagatcatg aaatcaaatc cgttggtgtc gcacatgcta acgtcattga
    38581 atatgttgat aaattgaaga aagtttttaa tgaagctttt catgtggata attacgatat
    38641 aaatgtaact acaccagtta tttctgcaca tactggtcaa ggtgcgattg gcctcgtagt
    38701 ccttaagaag taaatttaat cttttcagtg ttaattactt ccatttcaat cctttataga
    38761 ctaaatttat aattagatag atagaggagg taattcatat gacaaaagaa tatgcaacat
    38821 tagcaggagg atgtttctgg tgcatggtta aaccatttac atcatatcca ggcatcaagt
    38881 cagtcgtatc tggttatagt ggcggtcatg ctgataaccc aacttatgaa caggtatgta
    38941 cgaatcaaac cggccatgtc gaagcagtac aaattacgtt tgatccagag gttacttcct
    39001 ttgaaaatat attagacata tatttcaaaa catttgaccc aaccgatgat caagggcaat
    39061 ttttcgatag aggcgaaagc tatcaaccag tcattttcta tcatgataag catcagaaaa
    39121 aggctgctga gtttaaaaag caacaattaa atgaacaagg tattttcaag aaaccagtga
    39181 ttacacctat taaaccatat aaaaatttct atccagctga agactaccat caagattatt
    39241 ataaaaagaa cccggtacat tattaccaat atcaacgtgg ttcaggtaga aaagcgttta
    39301 tagaatcaca ttgggggaat caaaatgctt aaaaaagata aaagtgaact aacagatata
    39361 gaatatattg ttacacaaga aaatggcact gaaccaccat ttatgaatga atattggaat
    39421 cattttgcta aagggattta tgtagataag atttctggta aacctttatt tacatctgaa
    39481 gaaaagtttc attctgagtg cggatggcct agcttttcca aagcgcttga tgacgatgaa
    39541 attatagaat tagtcgacaa atcatttggt atggtaagaa ctgaagtgcg ttcagaagaa
    39601 tcaaatagtc atttaggaca cgtctttaat gatggaccta aagaaagtgg cggcttaaga
    39661 tactgtatca attccgctgc aattcaattt attccatatg aaaaattaga agaattgggt
    39721 tatggcgatt taatatcaca ttttgataag taggagtgga aagtatgttt aaaaaattat
    39781 tcggaaaagg caaagaagtt cagaaagata tcgcaattta tgcaccatta actggagaat
    39841 acgtgaaaat tgaagatatt ccagatcctg tattcgcaca aaaaatgatg ggcgaagggt
    39901 ttggtattaa tccaactgaa ggagaagttg tgtctccaat tgcaggacgt gttgacaatg
    39961 tctttccaac taagcatgca attggtttaa aagcagataa cggcttagaa ttattagttc
    40021 atatcggttt agacacagtt caattagacg gtgaaggatt tgaagtgtta gtgtctagtg
    40081 gtgatgaagt taacgtcgga gatccattag taagattcaa ccttgaatat attaataata
    40141 atgctaaatc tgttatttca ccaattataa ttactaacac tgatcaagcg gcttctatta
    40201 atatttatga tgaaaatgct gtgattaaag gtgaaactaa agtgattgat gtgacaatga
    40261 actaatgaaa aactattcgt tttatcaatt tgtcatgaca gtgcgtggtc gacacgacga
    40321 taaaggtcgt ctagcagaag agatatttga cgatcttgct ttcccaaaac acgatgatga
    40381 ttttaacata ctgtctgatt atattgagac acatggtgat ttcacattgc caatgtctgt
    40441 atttgatgat ctatatgaag aatatacgga atggctaaaa ttttaat
//
LOCUS       DATTQF010000010        36411 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.010, whole genome shotgun sequence.
ACCESSION   DATTQF010000010 DATTQF010000000
VERSION     DATTQF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.010"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..128
                     /locus_tag="VB241_000410"
     CDS             <1..128
                     /locus_tag="VB241_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HEP1243742.1"
                     /translation="INITDENDLYTALDILNQSIYEWIEENTDEQDKLINLVMKW"
     gene            143..622
                     /locus_tag="VB241_000411"
     CDS             143..622
                     /locus_tag="VB241_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEP1243743.1"
                     /translation="MANLYELSEAFKELSNQDELDPTLLKDTLDSIKAEMNVKVDNIV
                     NWRRETLGDIDVIDKEIKRLQNLKKQKQNLTDRLRDYLKEMLETQEVDSYRTATNHIY
                     KRKNGASKNIIDEKLIPKDYWLSQAPKLNSKQLIDDLKAGKDIPGAELKVTESLVIK"
     gene            622..1395
                     /locus_tag="VB241_000412"
     CDS             622..1395
                     /locus_tag="VB241_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1243744.1"
                     /translation="MSEEQDILQELGIEEINEDTQNYYSIMVYGKSGTGKTTLATREN
                     NAFIIDIHEDGTQVTRQGFVKRVDNYIAFRNTIASIESIVNTARQKGKLLDVVVIETA
                     QKLRDITLTHVMNTHQVKKARIQDYGETSKLIVNSIRHLLKVKDKLGFHVVLTGHEGL
                     NSEDKDENGKIINPRISIEVQPAIHNNLVTQFDIIGHTFIEDHTDENGNATHDYVFSV
                     EPSNLYTTKVRHNPQITINNPGIKNASISKIIDMAQNGN"
     gene            1427..1981
                     /locus_tag="VB241_000413"
     CDS             1427..1981
                     /locus_tag="VB241_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1243745.1"
                     /translation="MKITGRTQYIQETNQEAFMKGGDFLGAGEFTVEVANVEFNDREN
                     RYFTIVFENNEGKQYKHNQFVPPFQQDYQEKQYIELLSRLGIKLNLPDLTFDTDQLIN
                     KIGTIVLKNKFNEEQGKYFVRLSYVKVWNKDDEVVNKPEPKTDEMKQKEQQANGKQTP
                     MSQQSNPFANANGPIEINDDDLPF"
     gene            1994..2665
                     /locus_tag="VB241_000414"
     CDS             1994..2665
                     /locus_tag="VB241_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243746.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESIRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSIETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWLKVDERLNKMLK
                     GEKEK"
     gene            complement(2775..3434)
                     /locus_tag="VB241_000415"
     CDS             complement(2775..3434)
                     /locus_tag="VB241_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016028252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243747.1"
                     /translation="MNNLKKCFIACPIGTDDSKVRRNSDFLLQSIIKPALESDFEIQR
                     SDLISSTNKITDEIIGGLTNSELVIVDLSTHNPNVFYELGYRHALERPTITMINKDEN
                     IPFDVSAYRTIYYSELYADVVNAKDQLKETIKTFTDNDFNFENPVNKYNNIDNEYGVL
                     NRHLLDIKSDLSELKEFLPSVTKQDPDIPADSMVRMMELAVQYPDQFERLMELQNKNS
                     Q"
     gene            3480..4250
                     /locus_tag="VB241_000416"
     CDS             3480..4250
                     /locus_tag="VB241_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEP1243748.1"
                     /translation="MATFRTIKESGDFVTVHKSFVFDSNLSAKAKGILLYFLSRPDNW
                     QIYTSEVVKHMNDGQKSINSGVQELMDNKYVHRIQKRAENGVFKGFEYLVYEKPTEMP
                     FSENGLSANGFSENGKPENRKGRTTNNNSTNNDLTNNNNTNNDGSILSGNPTVYSIPY
                     KEIIEYLNKKTGKHFKHNTAKSKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKY
                     LRPETLFGNKFEGYLNQKIQPTGTDQLERMKYDESYWD"
     gene            4260..5033
                     /locus_tag="VB241_000417"
     CDS             4260..5033
                     /locus_tag="VB241_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1243749.1"
                     /translation="MKPLFDEKINKSLKKYQPIEVILRQNCDKCGHQYDLYKFENGYE
                     YKDGCECEIQRLAYEEYKRNKQKKLDYIFNQSNVNPSLRDATVNNYKPQNEKQVQAKQ
                     TAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDRIK
                     ATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTN
                     FSDKELNQNMNWQRINSRMKKRARKVRVTGDDFRERDAW"
     gene            5027..5188
                     /locus_tag="VB241_000418"
     CDS             5027..5188
                     /locus_tag="VB241_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243750.1"
                     /translation="MVITKQNIKEILHCRDVYAQKMIDFANGDQEKLKKLIDDKLKEK
                     EERPAIVEY"
     gene            5201..5422
                     /locus_tag="VB241_000419"
     CDS             5201..5422
                     /locus_tag="VB241_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HEP1243751.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNVNEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            5432..5857
                     /locus_tag="VB241_000420"
     CDS             5432..5857
                     /locus_tag="VB241_000420"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HEP1243752.1"
                     /translation="MEVHYSSKTNEWTTPQHLFDDLNEEFSFTLDPCSTDENAKCRKY
                     YTVKDNGLIQDWSEDIVFMNPPYGRSIKRWVKKAYEESLKGATVVCLIPARTDTTYWH
                     DYIFNKADDIRFLRGRLKFGDSKNSAPFPSAIIVYRGAQ"
     gene            5854..6252
                     /locus_tag="VB241_000421"
     CDS             5854..6252
                     /locus_tag="VB241_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEP1243753.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGANYDRIELQP
                     KFELQPKFGKQRPITYIADFSLWKEGKLVEVVDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKMK"
     gene            6257..6442
                     /locus_tag="VB241_000422"
     CDS             6257..6442
                     /locus_tag="VB241_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HEP1243754.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDKEKEVLADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            6443..6805
                     /locus_tag="VB241_000423"
     CDS             6443..6805
                     /locus_tag="VB241_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243755.1"
                     /translation="MGSVVIINNKPYKFNNFEKEIMAKRGINAGIVSKRVRGCWEFSE
                     ALDAPYGMHLKEYREMKQMEKIKQARLERELERERKKEAELKRKKPHLFNVPQKHSRD
                     PYWFDNTYNQMFKKWQEA"
     gene            6806..7054
                     /locus_tag="VB241_000424"
     CDS             6806..7054
                     /locus_tag="VB241_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HEP1243756.1"
                     /translation="MPKTDNARKEYLNHFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQSVKKTFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            7068..7301
                     /locus_tag="VB241_000425"
     CDS             7068..7301
                     /locus_tag="VB241_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HEP1243757.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQEND"
     gene            7294..7854
                     /locus_tag="VB241_000426"
     CDS             7294..7854
                     /locus_tag="VB241_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HEP1243758.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIETKNPKDTHKAYVEEFFEWY
                     NTIEPFKNWKKIKGKPIEEQLDELSDMLAFALSYVLMTESYEELETYFCKIPELTNQY
                     SFLAKLHKVISVTEYHKLNKQYERTGDLEINHILDLLTDVELVLPFQIAVEYYSIDQL
                     IDAYKKKMKRNHERQDGTADAGKGYV"
     gene            7891..8127
                     /locus_tag="VB241_000427"
     CDS             7891..8127
                     /locus_tag="VB241_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HEP1243759.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            8152..8388
                     /locus_tag="VB241_000428"
     CDS             8152..8388
                     /locus_tag="VB241_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243760.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            8381..8554
                     /locus_tag="VB241_000429"
     CDS             8381..8554
                     /locus_tag="VB241_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HEP1243761.1"
                     /translation="MIKQILRLIFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            8555..8956
                     /locus_tag="VB241_000430"
     CDS             8555..8956
                     /locus_tag="VB241_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243762.1"
                     /translation="MYSKESIVNMIGTHKMKCNVLADVIPEYDSNSIAQYGIQATLPK
                     PQGENSSKVEDVVVRLERANKRYAQMLKEVEFINQSQQRLGHVDFCFLELLKKGYNRD
                     AIIKKMPNSKLNRNNFLARRDELAEKIYLLQ"
     gene            9311..9805
                     /locus_tag="VB241_000431"
     CDS             9311..9805
                     /locus_tag="VB241_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP1243763.1"
                     /translation="MIKLTPKQEKFVLGLIEGKSQRKAYIDAGYSTKGKSGEYLDKEA
                     STLFKNRKVSGRYEKLRQEVAEQSKWTRQKAFEEYEWLKNVAKNDIEIEGVKKATADA
                     FLASLDGMNRMTLGNEVLANKKIETEIKMLEKKIEQIDKGDSGTEDKIKQLHDAITEV
                     IVNE"
     gene            9798..11030
                     /locus_tag="VB241_000432"
     CDS             9798..11030
                     /locus_tag="VB241_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP1243764.1"
                     /translation="MNKLKSLYTDKQIEILKQTQKRDWFMLINHGAKRTGKTILNNDL
                     FLRELMRVRKIADEEGIETPQYILAGATLGTIQKNVLIELTNKYGIEFNFDKYNSFML
                     FGVQVVQTGHSKVSGIGAIRGMTSFGAYINEASLAHEEVFDEIKSRCSGTSARILVDT
                     NPDHPEHWLLKDYIENTDPKAGILSHQFKLDDNNFLNDRYKESIKASTPSGMFYERNI
                     NGMWVSGDGVVYADFDLNENTIKADELDDIPIKEYFAGVDWGYEHYGSIVLIGRGIDG
                     NFYFIEEHAHQFKFIDDWVVIAKDIVSRYGNINFYCDTARPEYITEFRRHRLRAINAD
                     KSKLSGVEEVAKLFKQNKLLVLYDNMDRFKQEVFKYVWHPTNGEPIKEFDDVLDSLRY
                     AIYTHTKPERLRRGNDIV"
     gene            11017..12441
                     /locus_tag="VB241_000433"
     CDS             11017..12441
                     /locus_tag="VB241_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP1243765.1"
                     /translation="MTLYKLIDDIEAQGILPKHIEALIESHKDDRERMVNLYNRYKTH
                     IDYVPIFKRRPIEEKEDFETGGNVRRLDVSVNNKLNNSFDSEIVDTRVGYLHGVPVTY
                     DLDENAEKNEKLKKFITNFAIRNSVDDEDSEIGKMAAICGYGARLAYIDTNGDIRIKN
                     IDPYNVIFVGDNILEPTYSLRYFYEKDDDNGTDYVYAEFYDNTYYYVFRGEGIDALQE
                     VGRYEHLFDYNPLFGVPNNKEMIGDAEKVIHLIDAYDLTMSDASSEISQTRLAYLVLR
                     GMGMSEEMIQETQKSGAFELFDKDMDVKYLTKDVNDTMIENHLDRIEKNIMRFAKSVN
                     FNSDEFNGNVPIIGMKLKLMALENKCMTFERKMTAMLRYQFKVILSALKRKGYNLDDD
                     SYLNLIFKFTRNIPVNKLEESQVLINLKGQVSERTRLGQSQLVDDVDYELDEMEKESL
                     EFNDKLPDIDEGDANDKSQNNQSE"
     gene            12410..13363
                     /locus_tag="VB241_000434"
     CDS             12410..13363
                     /locus_tag="VB241_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEP1243766.1"
                     /translation="MTNPKITNQNDIDEYIEGLISKAEKPIEQLFANRLKEIKQIIAD
                     MFEKYQNDDVYVTWTEFNKYNRLNKELTRIGTMLTDDYRQVAKMIQKSQEDAYIEKFL
                     MSLYLYEMASQTSMQFDVPSKEVIKSAIEQPIEFIRLMPTLQKHRDEVLKKIRMHITQ
                     GIMSGEGYSKIAKAIRDDIGMSKAQSLRVARTEAGRAMSQAGLDSAMVAKDNGLKMKK
                     RWHATKDTRTRDTHRHLDGESVEIDQNFKSSGCVGQAPKLFIGVNSAKENINCRCKLL
                     YYIDENELPTVMRARKDDGKNEVIPFMTYREWEKYKRKGGN"
     gene            13365..13571
                     /locus_tag="VB241_000435"
     CDS             13365..13571
                     /locus_tag="VB241_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243767.1"
                     /translation="MDFKIKVNVDTGEAIEKLERIKSLYEEIIELQNEKVGVNVTVKN
                     EADLDMVKTSISEENAKNNDFTLF"
     gene            13674..14258
                     /locus_tag="VB241_000436"
     CDS             13674..14258
                     /locus_tag="VB241_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEP1243768.1"
                     /translation="MNIEEVKSFFEEHKDDKEVKDYLNGLKTVSVDDVKGFLDTEEGK
                     RFIQPELDRYHSKGLESWKEKNLENLIEQEVQKRNPEQSEEQKRISALEKELEKRDAE
                     AKREKLRSNALGKAQELNLPTSLVDRFLGDSDEDTEQNLKALKETFDKYVQKGVESKF
                     KSSGRDVKESQNQDLDPSNVKSIEEMAKEINIRK"
     gene            14275..15189
                     /locus_tag="VB241_000437"
     CDS             14275..15189
                     /locus_tag="VB241_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEP1243769.1"
                     /translation="MATPTYTPGNVILSDFKNGVIPAEQGTLIMKDIMANSAIMKLAK
                     NEPMTAQKKKFTYLAKGVGAYWVSETERIQTSKPEYAQAEMEAKKIGVIIPLSKEFLK
                     WTAKDFFNEVKPLIAEAFYKAFDQAVIFGTKSPYNTSTSGKPLVEGAEEKGNVVTDTN
                     NLYVDLSALMATIEDEELDPNGVLTTRSFRSKMRNALDANDRPLFDANGNEIMGLPLS
                     YTGADVYDKKKSLALMGDWDYARYGILQGIEYAISEDATLTTLQASDASGQPVSLFER
                     DMFALRATMHIAYMNVKPEAFATLKPTE"
     gene            15201..15344
                     /locus_tag="VB241_000438"
     CDS             15201..15344
                     /locus_tag="VB241_000438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243770.1"
                     /translation="MANPAEEIKVKKDNMTITVTKKAFDSYYSLVGYKEVKSRRTTSD
                     KSE"
     gene            15350..15700
                     /locus_tag="VB241_000439"
     CDS             15350..15700
                     /locus_tag="VB241_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEP1243771.1"
                     /translation="MTLYEDVKLLLKKNGVEVKSDEEEIFKMEVDGILEDVRDITNND
                     FMKDGQVIYPYSIKKYVADVLEYYQRPEVKKNLKSRSMGTVSYTYNDGVPDYISGVLN
                     RYKRAKFHPFKPIR"
     gene            15712..16047
                     /locus_tag="VB241_000440"
     CDS             15712..16047
                     /locus_tag="VB241_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEP1243772.1"
                     /translation="MFNPYDEFPHTISIGSIKKVGEYPIIQERFVSDKTIKGFMDTPT
                     TSEQLKFHQMSQEYDRNLYVPYDLPISKNNLFEYEGRIFSIEGDSVDQGGQHEIKLLR
                     LKQVPYGKS"
     gene            16034..16447
                     /locus_tag="VB241_000441"
     CDS             16034..16447
                     /locus_tag="VB241_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP1243773.1"
                     /translation="MAKVKYGADSMVVELDKFDKKIEEWVKKGIAKTTTKIYNTAVAL
                     APVDLGFLEESIDFKYFDGGLSSVISVGADYAIYVEYGTGIYATGPGGSRATKIPWSF
                     KGDDGEWYTTYGQAPQPFWNPAIDAGRKTFEQYFS"
     gene            16460..16885
                     /locus_tag="VB241_000442"
     CDS             16460..16885
                     /locus_tag="VB241_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEP1243774.1"
                     /translation="MWVSVEPELTNQIYKRLISDPNINKLVDDRVFDVVQDDAVYPYI
                     VVGESNVTNNESSATMRETVGIVIHVYSQFATQYEAKLILSAIGYVLNRPIEIDNYEF
                     QFSRIDSQAVFPDIDRFTKHGTIRLLFKYRHKKKNEGVY"
     gene            16886..17443
                     /locus_tag="VB241_000443"
     CDS             16886..17443
                     /locus_tag="VB241_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1243775.1"
                     /translation="MAQKNYLAVVRPAETDLDPVESLLLADLQEGGHTIENDLAEIVR
                     GGKTDYSPNAMSESFKLTIGNVPGDKGIEAVKHAVQTGGQLRIWLYERNKRADGKHHG
                     MFGYVVPESFEMSFDDESDKIELSLKVKWNTAEGAEDNLPKEWFEAAGAPTVEYEKFG
                     EKVGTFENQKKASVVSDSHTEDHSM"
     gene            17510..18016
                     /locus_tag="VB241_000444"
     CDS             17510..18016
                     /locus_tag="VB241_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243776.1"
                     /translation="MTEFNPITTLKINDGEKDYEVEAKVTFAFDRKAEKFSEDSEDGR
                     KGAMPGFNVIFNGLLESRNKAILQFWECATAYLKNPPTREQLEKAIDDFITENEDTLP
                     LLQGALDKLNNSGFFKRESRSYWMTLNKAPNMAKSEDKEMTKAGIEMMKENYKEIMGA
                     EPYTITQK"
     gene            18061..18345
                     /locus_tag="VB241_000445"
     CDS             18061..18345
                     /locus_tag="VB241_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243777.1"
                     /translation="MLALTPAEWRDWLIGGQDRYLDQRQLLIEQAQANGLVQASKRLT
                     SMIRDIEKQRYEIREPGSYARVQKARLEEEKRRRELFKEGTRKFLESKGG"
     gene            18349..21492
                     /locus_tag="VB241_000446"
     CDS             18349..21492
                     /locus_tag="VB241_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEP1243778.1"
                     /translation="MDTHFMAKIMANIRDFQSNVRKAQRLAKTSVPNEIETDVKADIS
                     RFQRALQRAKSMAQRWREHSVKLFMKTDEYKANLERAKAQVERFKQHKVDLKLSNTEL
                     MAKYNATKATVEAWRKHVVKLDLDANPAKMAVKGFKEDLIDLSRHSFDIDSSRWKLGN
                     KFTKEFNEVEGAVKRSFGRIGQIMRKEVNGTSDIWGKLNNSLKDYGEKMDALATKIRT
                     FGTIFAQQVKGLMIASIQALIPVIAGLVPAIMAVLNAVGVLGGGVLGLVGAFSVAGLG
                     VVGFGAMAISALKMVEDGTLAVTKEVQNFRDASDQLKTTWRDIVKENQASIFNAMSAG
                     IRGVTSAMSQLKPFLSEVSMLVEANARKFEDWVKHSETTKKAFEALNSIGGAIFGDLL
                     NAAGRFGDGLINIFTQLMPLFKFVSQGLQNMSIAFQNWANSVAGQNAIKAFIDYTTTN
                     LPKIGQIFGDVFAGIGNLMIAFAQNSSNIFDWLVKLTSQFRAWSEQVGQSQGFKDFIS
                     YVQENGPTIMQLIGNIVKALVAFGTAMAPIASKLLDFVTNLAGFIAKLFEAHPAVAQI
                     IGVIGILGGVFWALMAPIAAVSSVLSNVFSMTLLNVVKRILDLTRITGVVSKAFGLLT
                     GAFTSISWPILAVVAVIGVFIGILVYLWKTNENFRKTITEAWNGIKTAVSGAIQGVVD
                     WLTQLWGKIQSTLQPIMPILQVLGQVFMQVLGVLVIGIITNVMNIIQGLWTLITIAFQ
                     AIGTVISVAVQIIVGLFTTLIQLLTGDFSGVWETIKTTVTNVLDTIWQYMQSVWASII
                     GFLTGVMNRTLSMFGTSWSQIWSTITNFVSSIWSTVTSWFSRVAWSVAEKMGQALNFI
                     ITKGSEWVSNIWNTVTSFASKVADGFKRVVSNVGDGMSDALGKIKSFFSDFLNAGAEL
                     IGKVAEGVANAAHKVVSAVGDAISSAWDSVTSFVSGHGGGSSLGKGLAVSQAKVIATD
                     FGSAFNKELSSTLTDSIVNPVSTSIDRHMTSDVQHSLKENNRPIVNVTIRNEGDLDLI
                     KSRIDDMNAIDGSFNLL"
     gene            21507..22448
                     /locus_tag="VB241_000447"
     CDS             21507..22448
                     /locus_tag="VB241_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP1243779.1"
                     /translation="MIAHDIEVIRNGSQYRVSDNPFTYNHLEVVEYNVTGAGYHRNYS
                     DIEGIDGRFHNYAKEELKKVELKIRYKVPKIAYASHLKSDVQALFAGRFYLRELATPD
                     NSIKYEHILDIPKDKQAFELDYVDGRQLFVGLVSEVSFDTTQTSGEFSLSFETTELPY
                     FESVGYSTDLESNNDPEKWSVPDRLPTNEGDKRRQMTFYNTNSGEVYYNGDVPLTQFN
                     QFNVVEIELAEDVKANDKDGFTFYTDKGNISVIKEVDLKAGDKIIFDGKHTYRGYLNI
                     DSFNKTLEQPVLYPGWNRFKSNKVMKQITFRHKLYFR"
     gene            22459..24345
                     /locus_tag="VB241_000448"
     CDS             22459..24345
                     /locus_tag="VB241_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1243780.1"
                     /translation="MPILLKSLQGVGHAINVSTKVSKKLNEDSSLDLTIIENASTFDA
                     IGAITKMWTITHVEGEDDFNEYVIVILDKSTIGEKIRLDIKARQKELDDLNNSRIYQE
                     YNESFTGVEFFNTVFKGTGYKYVLHPKVDASKFEGLGKGDTRLEIFKKGLERYHLEYE
                     YDAKTKTFHLYDELSKFANYYIKAGVNADNVKIQEDASKCYTFIKGYGDFDGQQTFAE
                     AGLQIEFTHPLAQLIGKREAPPLVDGRIKKEDSLKKAMELLIKKSVTASISLDFVALR
                     EHFPEANPKIGDVVRVVDSAIGYNDLVRIVEITTHRDAYNNITKQDVVLGDFTRRNRY
                     NKAVHDAANYVKSVKSTKSDPSKELKALNAKVNASLSINNELVKQNEKINAKVDKMNT
                     KTVTTANGTIMYDFTSQSSIRNIKSIGTIGDSVARGSHAKTNFTEMLGKKLKAKTTNL
                     ARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGTDKTD
                     TKTFYGAFCSAIEVIRKNNPDSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTLEDYV
                     NAQILACSELDVPVFDAYHTDYFKPYNPAFRKASMEDGLHPNEKGHEVIMYELIKDYY
                     SFYD"
     gene            24358..26256
                     /locus_tag="VB241_000449"
     CDS             24358..26256
                     /locus_tag="VB241_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243781.1"
                     /translation="MAYGLITSLHSMTGRKIVAQHEYNYRLLDEGMSKLEKMFIYHQK
                     EEIYAHSAKQIKYLNDSVEDYLTYLNGRFSNMILGHNGDGINEVKDARIDNTGYGHKT
                     LQDRLYHDYSTLDAFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYTNAVM
                     QSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIDGEL
                     WIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENLMVFR
                     REYKTSEQQAKNALNFVEVRSADDIDKGIDKVLYQMDIPMQYTSGTQPMQGIAYDAGI
                     LYWYTGDSNPANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMYYDLE
                     TGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPIQNP
                     AYLSDITEVGHYYIYTQDTQNALDFPLPRAFRDAGWFFDVLPGHYNGALRQVLTRNST
                     GRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLGFYITTEE
                     SKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGVGKWS
                     LFEGKVVE"
     gene            26256..28079
                     /locus_tag="VB241_000450"
     CDS             26256..28079
                     /locus_tag="VB241_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEP1243782.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFDK
                     IVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKITDDYGKAIESSEQSIDSVLS
                     AINTSRIIHITSATDAPTFKDIGTLETPKEDGVDDGSEVSATTNTLGKSGLLVVYVVD
                     DSTARATWYPDDSNDEYTKYKIGGTWYQFYKKVDEELTKKFVEETSNNALNQAKQYVD
                     DKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGY
                     LSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGT
                     TINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECL
                     LSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK"
     gene            28079..28456
                     /locus_tag="VB241_000451"
     CDS             28079..28456
                     /locus_tag="VB241_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HEP1243783.1"
                     /translation="MKITVNDKNEVIGFVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASKQQSESDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            28460..28633
                     /locus_tag="VB241_000452"
     CDS             28460..28633
                     /locus_tag="VB241_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEP1243784.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPEAKDEKSQV"
     gene            28674..28973
                     /locus_tag="VB241_000453"
     CDS             28674..28973
                     /locus_tag="VB241_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HEP1243785.1"
                     /translation="MFGFTKRHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIK
                     LDKTLDALQRERQIDEKNKKENDKNIRDMKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            29110..31008
                     /locus_tag="VB241_000454"
     CDS             29110..31008
                     /locus_tag="VB241_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP1243786.1"
                     /translation="MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTT
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGI
                     YFTRDKYK"
     gene            31021..32193
                     /locus_tag="VB241_000455"
     CDS             31021..32193
                     /locus_tag="VB241_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEP1243787.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPGTK
                     MWQIDGKDTNIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFEKFKETLNDLYAPKNHNH
                     DERYVLSSQAFTKEEADKLYQPIGSSQPSLNIWTGSETEYNYLYQKDPNTLYLIKG"
     gene            32198..32593
                     /locus_tag="VB241_000456"
     CDS             32198..32593
                     /locus_tag="VB241_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243788.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRVSQTNLGSSDLGISGVNSGVAS
                     KNINFSNPSGMLYVTISDVYSGSPTLTIE"
     gene            32649..33086
                     /locus_tag="VB241_000457"
     CDS             32649..33086
                     /locus_tag="VB241_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP1243789.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQITDLFGFDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSNKEEQQVTWKSSQDSSLT
                     PELSTKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKID"
     gene            33067..34512
                     /locus_tag="VB241_000458"
     CDS             33067..34512
                     /locus_tag="VB241_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP1243790.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQFNIDLWYAFQCFDYANAGWKALF
                     GLLLKGVGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQLGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKQLQLKDYFIKQIRVYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKIGPFLSCPVAYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            35468..36025
                     /locus_tag="VB241_000459"
     CDS             35468..36025
                     /locus_tag="VB241_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001657239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243791.1"
                     /translation="MGNTTYLKINSENDVDLQDILNDFINCFCKGYVEIKTKYKLLPI
                     FKINFHKNNLPHLLGLHYTHKKVSAKKIIGRIAEGKITHESIKKHYEYSNIKDRLINY
                     NFLHKCFIDKEIRLCVIVPKNSINPQKIDVAFIDDKNSQVMILGLRKSNNNDFYSPAT
                     MYVLGKNSSYRRMRRTHVISIEWKN"
BASE COUNT        13442 a         5478 c         7428 g        10063 t
ORIGIN      
        1 gtataaatat aacagatgag aatgatctat acacagcatt agacatatta aatcaatcta
       61 tttatgaatg gattgaagag aacacagacg aacaggacaa actaattaac ttagtcatga
      121 aatggtagag ggggattaac taatggctaa tctatatgag ctatcagaag catttaaaga
      181 gttgtctaat caagatgaat tagatccaac attactaaaa gacacattag attctatcaa
      241 agcagaaatg aatgtcaaag tagataacat cgtcaattgg agacgtgaaa cattaggtga
      301 catagatgtc atagataaag agattaagag acttcaaaat ttaaaaaaac aaaaacaaaa
      361 tttaactgat cgattaagag attacttaaa agagatgtta gaaacacagg aagtagatag
      421 ttaccgcaca gctactaatc atatttacaa gcgtaaaaac ggggctagta aaaatattat
      481 cgatgaaaaa cttattccaa aggattattg gctatcacaa gccccgaaac ttaattctaa
      541 gcaactaatc gatgatttga aagctggcaa agatattccg ggtgctgaat taaaggtaac
      601 ggaaagtttg gtgattaagt gatgagtgag gaacaagaca ttttacaaga actaggtatt
      661 gaagaaatta acgaagatac tcagaactat tattcaatta tggtatatgg caaatcagga
      721 accggaaaga cgactttagc cactagagaa aacaacgctt ttattatcga tattcacgaa
      781 gatggtactc aagtaacgcg acaaggtttt gtgaagagag tcgacaatta cattgctttt
      841 agaaacacaa ttgcgagtat tgaatcgatt gtaaatacag ctagacaaaa aggaaagtta
      901 cttgatgtgg ttgtaattga aacagctcaa aaattaagag atataacgct aactcatgta
      961 atgaatacgc atcaagtcaa aaaagcgaga atacaagatt atggggaaac atctaaattg
     1021 atcgttaact cgattaggca cctattaaag gttaaagata agctcggatt tcacgttgtg
     1081 cttacaggac atgaagggct taactcggaa gataaagatg agaacggaaa aattattaac
     1141 cctagaatat caattgaagt acaaccggca atacacaata acttagtaac tcagttcgac
     1201 attataggac atacatttat agaagatcat acagatgaga acggaaatgc gacacacgac
     1261 tatgtatttt ctgtagaacc ttctaattta tatacaacta aagttaggca taatccgcaa
     1321 ataacaatca ataatccagg tattaaaaat gcttcaattt caaaaattat agatatggca
     1381 caaaacggaa attaataaaa aactaaaaaa ggacggtata aaaattatga aaatcactgg
     1441 tagaacacaa tacattcaag aaactaatca agaggcattc atgaaaggtg gggacttttt
     1501 aggagctgga gaatttacag tagaagttgc aaatgtcgag tttaacgaca gagaaaacag
     1561 atacttcacg attgtttttg aaaacaacga aggtaaacaa tacaaacaca accaattcgt
     1621 cccaccattc caacaagatt atcaagaaaa acaatatatc gagttactta gtagattagg
     1681 aattaaattg aacttaccag atttaacttt tgacacagat caattaatta acaaaatcgg
     1741 aactattgta cttaaaaata aatttaacga ggaacaaggc aagtattttg taagactctc
     1801 atatgtaaaa gtttggaata aagacgatga agtagttaat aaaccagaac ctaaaactga
     1861 tgagatgaaa caaaaagaac agcaagcaaa tgggaaacag acgccaatga gtcaacaatc
     1921 aaacccattc gctaatgcta atggtccaat agaaatcaat gatgatgatt taccgttcta
     1981 ggacgtggtt taaatgcaat acattacaag ataccagaaa gacaatgacg gtacttattc
     2041 cgtcgttgct actggtgttg aacttgaaca aagtcacatt gacttactag aaaacggata
     2101 tccactaaaa gcagaagtag aggttccgga caataaaaaa ctatctatag aacaacgcaa
     2161 aaaaatattc gcaatgtgta gagatataga acttcactgg ggagaaccgg tggaatcaat
     2221 tagaaaatta ttacaaacag aattggaaat tatgaaaggt tatgaagaaa tcagtctgcg
     2281 cgactgttct atgaaagttg caagggagtt aatagaactg attatagcgt ttatgtttca
     2341 tcatcaaata cctatgagca tagaaacaag caagttgtta agtgaagata aagcactatt
     2401 gtattgggct acaatcaacc gcaactgtgt aatttgtgga aagcctcacg cagacctagc
     2461 gcattatgaa gcagtcggca gaggcatgaa cagaaacaaa atgaaccact atgacaaaca
     2521 tgtattagcg ttatgtcgcg aacatcacaa cgagcaacat gcgattggcg ttaagtcgtt
     2581 tgatgataaa tatcacttgc atgactcatg gctaaaagtt gatgagaggc tcaataaaat
     2641 gctgaaagga gagaaagaga aatgaacaaa atattaatac gctttgctat taactatata
     2701 aaatatcaac aaaaacaatt gcgtgaaaaa gaagcccgaa ttaaatatct agagggcttc
     2761 ttaaaaggga agggttattg actgtttttg ttttgcaatt ccatcaatct ttcaaattga
     2821 tctggatact gaacggcaag ttccatcatt cttaccatag aatcagcggg aatatcaggg
     2881 tcttgtttgg tcactgaagg tagaaactct tttaattcgg ataaatcgct ttttatatct
     2941 aacaaatgtc tatttaaaac accgtattca ttatcgatat tattatactt attgactgga
     3001 ttttcaaaat tgaaatcatt atctgtaaat gttttgatag tttctttaag ttggtctttg
     3061 gcatttacta catccgcgta taattcgctg tagtaaattg tacggtaagc gctaacatca
     3121 aaagggatat tctcatcttt gttaatcata gtaattgttg gtctctctag agcgtgtctg
     3181 taacctaatt cataaaatac gttcgggtta tgcgtactca aatctacaat aactaattca
     3241 gaattcgtta atccaccaat tatttcatca gtgattttat tggtcgatga tataaggtca
     3301 gatcgttgga tttcaaaatc tgattctaaa gcaggtttta tgatggattg taaaagaaaa
     3361 tcagagtttc ttcttacttt agaatcatca gtacctatag ggcaagcaat gaaacatttt
     3421 ttcaagttat tcactcctaa tcatattttt attaattata acagaaagga gataaaaaaa
     3481 tggcaacatt tagaacgata aaagaaagtg gcgattttgt aactgtgcat aaatcttttg
     3541 tgttcgatag taatttaagt gctaaagcta aagggatatt attgtatttc ctgagtcgtc
     3601 ctgacaattg gcaaatatac acgtcagaag tagttaaaca tatgaatgat ggacaaaaat
     3661 caatcaatag tggcgttcaa gaacttatgg ataataaata tgttcacaga atacaaaaaa
     3721 gagctgaaaa cggtgtgttt aaaggttttg aatacttagt ttacgaaaaa ccaaccgaaa
     3781 tgccattttc ggaaaacgga ttatcggcaa acgggttttc ggaaaacgga aaaccggaaa
     3841 accgaaaagg gcgtactact aataataata gtactaataa tgatttaact aataataaca
     3901 atactaataa tgatggaagt atattgtcgg gaaacccgac tgtgtattcc attccctata
     3961 aagaaattat cgaatactta aacaaaaaaa caggaaagca ttttaaacac aatacagcta
     4021 aatcaaaaga ttttattaaa gcaagatgga atcaagattt taggttggag gattttaaaa
     4081 aggtgattga tatcaaaaca gctgagtggc taaacacgga tagcgataaa taccttagac
     4141 cagaaacact ttttggcaat aaatttgagg ggtacctcaa tcaaaaaata caaccaactg
     4201 gcacggatca attagaacgt atgaagtacg acgaaagtta ttgggactag gaggaagtta
     4261 tgaaaccatt atttgacgaa aaaataaaca aaagtttaaa aaaatatcaa ccaatcgaag
     4321 taatactaag acagaattgc gataaatgcg ggcatcaata tgacttatat aagtttgaaa
     4381 atggatatga atacaaagat ggttgcgaat gtgaaattca aagattggct tatgaagaat
     4441 acaaaaggaa taaacaaaag aaacttgatt atattttcaa tcaatcaaat gttaatccgt
     4501 ctttaagaga tgcaacagtt aacaactata agccacaaaa tgaaaaacaa gtacaagcta
     4561 agcaaacagc aatagagtac gtacaaggct tctctacaaa agaaccaaaa tcattaatat
     4621 tgcaaggttc atacggaact ggtaaaagcc acctagcata cgctatcgca aaagcagtta
     4681 aagctaaagg acatacggtt gcttttatgc acataccaat gttgatggat cgtatcaaag
     4741 cgacatacaa caaaaatgca gtagagacta cagacgagct agtcagattg ctaagtgata
     4801 ttgatttact tgtactagat gatatgggtg tagaaaacac agaacacact ttaaataaac
     4861 ttttcagcat tgttgataac agagtaggta aaaacaatat ctttacaact aactttagtg
     4921 ataaagaact aaatcaaaat atgaactggc aacgtataaa ttcgagaatg aaaaaaagag
     4981 caagaaaagt aagagtaacc ggagacgatt tcagggagcg agatgcgtgg taatcacaaa
     5041 acaaaatata aaagaaatat tacattgtag agatgtatat gctcaaaaga tgattgattt
     5101 tgcaaacgga gaccaagaga aacttaaaaa acttattgat gataagttga aagaaaaaga
     5161 agaaagaccc gcaatcgtcg aatattaagg agtgttaaaa atgccgaaag aaaaatatta
     5221 cttataccga gaagatggca cggaagatat caaagtcatc aagtataaag acaacgtaaa
     5281 tgaagtttat tcgctcacag gagcccattt cagcgacgaa aagaaaatta tgactgatag
     5341 tgacctaaaa cgattcaaag gcgctcacgg gcttctatat gagcaagaac taggtttaca
     5401 agcaacgata tttgatattt agaggtggca catggaagta cattacagta gtaaaacaaa
     5461 cgagtggaca acaccacaac atttatttga tgacctaaac gaagaattca gttttacatt
     5521 agatccttgt tcaacagacg agaacgccaa atgccggaag tattatacag taaaagataa
     5581 tgggttaatt caagactggt ctgaggacat tgtttttatg aacccgccat acggtcgaag
     5641 tattaagcgt tgggtcaaga aggcttatga agaaagtttg aaaggcgcaa cggtagtttg
     5701 tttaataccc gcaagaacag acacgacata ttggcatgat tacattttta ataaggctga
     5761 tgatataaga ttcctacgcg gtcgtctgaa gtttggagat agtaaaaaca gcgctccttt
     5821 tcctagcgca attatcgttt atagaggtgc acaatgagta aatacaacgc taagaaagtt
     5881 gagtacaaag gaattgtatt tgatagcaaa gttgagtgtg aatattacca atatttagaa
     5941 agtaatatga atggcgctaa ctatgatcgt atcgaactac aaccgaaatt cgaactacaa
     6001 cctaaatttg ggaagcaaag accgattacg tatatagccg atttctcttt gtggaaggaa
     6061 gggaaactgg ttgaagttgt agacgttaaa ggtaaggcga ctgaagttgc caacatcaaa
     6121 gcgaagatat tcagatatca gtatagagat gtgaatttaa cgtggatatg taaagcgcct
     6181 aaatacacag gtcaagaatg gatggtatat gaggacttag tgaaagtcag acgtaaaaga
     6241 aaaatgaagt gatttaatgc aacaacaagc atatataaac gcaacgattg atataaggat
     6301 acctacagaa gttgaatatc agcatttcga tgatgtggat aaagaaaaag aagtgttggc
     6361 agattactta tataacaatc cagacgaaat actagagtat gacaatttaa aaattagaaa
     6421 tgtaaatgta gaggtggaat aaatgggcag tgttgtaatc attaataata aaccatataa
     6481 atttaacaat tttgaaaaag aaataatggc aaagcgtggg ataaatgctg gaattgtttc
     6541 taaacgtgta agaggttgtt gggagttttc agaagcttta gacgcgcctt atggcatgca
     6601 tctaaaagaa tatagagaaa tgaaacaaat ggaaaaaatt aaacaagcga gactcgaacg
     6661 tgaattggaa agagagcgaa agaaagaggc tgagctaaaa agaaagaagc cacacttgtt
     6721 taatgtacct cagaaacatt cacgtgatcc gtactggttt gataatactt ataaccaaat
     6781 gtttaagaaa tggcaggaag cataaatgcc taaaaccgat aacgcacgca aagaatactt
     6841 aaaccatttt ttcggatcta agagatatct gtatcaggat aacgaacgag tggcacatat
     6901 ccatgtagtg aatggcactt attactttca cgggcatatc gtgccaggtt ggcaaagcgt
     6961 taaaaagaca tttgatacag ctgaagaact tgaaacatat ataaagcaac atggtttgga
     7021 atatgaggaa cagaagcaac taactttatt ttaaggagat gtaaacaatg aataaccgcg
     7081 aacaaatcga acaatccgtt atcagtgcta gtgcgtataa cggcaatgac acagagggat
     7141 tactaaaaga gattgaagac gtgtataaga aagcacaagc gtttgatgaa attcgcgaag
     7201 ctattaatgc gcaatgggtt gagtatccag aagactgggc gtcagaggtt ttgagagaag
     7261 taagagagct tgaatatgag gaggaacagg aaaatgacta acacattaac aattgatcaa
     7321 ttacaagagt tattacaaat acaaaaggag ttcgacgata gaattgaaac caaaaatccg
     7381 aaagatacgc acaaagcgta tgtagaagaa tttttcgaat ggtataacac aatagagcca
     7441 ttcaagaact ggaaaaagat aaaaggtaaa cctattgaag aacaacttga tgaactatca
     7501 gatatgttgg cttttgcact gtcctatgta ttgatgactg aaagttacga agagcttgag
     7561 acttattttt gtaaaatacc agaattaaca aatcaatata gttttttagc caaattacac
     7621 aaagttattt cagtcacaga atatcataaa ctaaataaac aatatgaacg tactggtgat
     7681 ttagaaatca atcatatttt agatttgttg acagatgtgg aattagtatt accttttcaa
     7741 atagctgtgg agtattactc tatcgaccaa ctcattgacg catacaaaaa gaaaatgaaa
     7801 aggaaccacg aaagacaaga tggaacagca gacgcaggaa aaggatacgt gtaaagacat
     7861 attagatcgg gtcaaggagg ttttggggaa gtgacacaat acttagtcac aacattcaaa
     7921 gattcaacag gacgcaagca tacacacata actcgagcta aaagcaatca aaggtttaca
     7981 gttgttgagg cagagagtaa agaagaagcg aaagagaaat atgagtcaca aaatacacct
     8041 attgtttact acactaataa ttctaaagtg accttattcg aaagacctag tgaagaagta
     8101 ttaggttctt tgttcgaaaa gaaataaaat cattaaagag gggagataat aatgtttaat
     8161 acacctaaaa tgaaattacc agaaaagcac accgaggtat ttaagacgta taaaaatgga
     8221 acgccagaag aaaaagctga gattgaaggc tgttttatta aaactgttaa agatgaagat
     8281 agtgaatttt acagccctat gttagccagt ctaaatgaac aacagttaaa gagtatgttg
     8341 agacaggtac tttttttgat tgatacagga gatgacaatg atgattaaac aaatattaag
     8401 actaatattc ttactagcaa tgtatgagct aggtaagtat gtaactgaac aagtatatat
     8461 tatgatgacg gctaatgatg atgtagaggc gccgagtgac ttcgcaaagt tgagcgatca
     8521 gtcagatttg atgagggcgg aggtgtcaga gtagatgtat agcaaagagt caattgttaa
     8581 tatgataggc acacataaaa tgaagtgtaa tgtattagct gatgtaatac cggaatatga
     8641 tagcaattca attgcacagt atggcataca agcaacgttg ccgaaaccac aaggggaaaa
     8701 ctcaagtaaa gttgaagatg ttgttgtgag gcttgagaga gcaaataaaa ggtatgctca
     8761 gatgttaaaa gaggttgagt ttataaatca atcgcaacag agattgggac acgttgactt
     8821 ttgtttctta gagttattga agaaaggtta taacagggat gcaattatca agaagatgcc
     8881 taactctaaa ttgaacagaa acaacttctt agcgcgccgt gatgaattag cagaaaagat
     8941 ttatctacta cagtgacgaa aatgacaaaa atgacagaaa tgacgaaaat gacactattt
     9001 ttaaactgtg aattaatttt atataattga tttgtaagaa ttatcttaag acgtggggta
     9061 atagccacaa cagatgttct catcgatgtg attaagaagt gacaaacata taaaagatga
     9121 tatgttacgc tattaatcac ttactacctg cctatatggt gggtagttta attcttgcat
     9181 tttgagtcat aactattttc ctcctttcac atttattgaa cgtagctcct gcacaagatg
     9241 taggggcatt tttttatatt taaaataact agagtaatta acgtaaaggc gtgtgataca
     9301 gtgaaaacaa ttgattaaat taacaccgaa gcaagaaaag tttgtgctag gactcataga
     9361 gggcaagagc caacggaaag catatattga cgcagggtat tcgactaaag gtaagagtgg
     9421 ggaatatcta gataaagaag cgagtacact ttttaaaaat cggaaggttt ccggaaggta
     9481 cgaaaaattg cgtcaagaag tagctgaaca atcaaaatgg acacgccaaa aggcctttga
     9541 agaatatgag tggctaaaga atgtagctaa gaatgacatt gaaatagagg gagtgaagaa
     9601 agcgacagct gatgcattcc tcgctagttt agatggtatg aatagaatga cgttaggtaa
     9661 cgaagtttta gctaacaaga aaatagaaac tgaaattaag atgcttgaga agaagattga
     9721 acaaatagat aaaggtgaca gtggaacaga agataaaatc aaacaacttc acgacgcaat
     9781 aacggaagtg atcgtcaatg aataaactta aatctttata tacggacaaa caaattgaaa
     9841 tattgaagca aacgcaaaaa cgagattggt ttatgttaat taatcacgga gcaaagcgta
     9901 caggtaaaac aatattaaac aatgacttat ttttacgtga gttaatgcgt gtgcgaaaga
     9961 tagcagacga agaaggaatt gagacacctc aatatatact tgctggtgca acattaggta
    10021 cgattcaaaa aaacgtacta atagagttaa ctaacaaata tggcattgag tttaattttg
    10081 ataaatataa ttcattcatg ttatttggcg ttcaagtggt tcagacaggt cacagtaaag
    10141 taagtggtat aggagctata cgtggtatga catcgtttgg tgcatatatc aatgaagcgt
    10201 cgttagcgca tgaagaggtg tttgacgaga ttaagtcacg ttgtagtgga actagtgcaa
    10261 gaatattggt agataccaac cctgaccatc ccgagcattg gttgttgaaa gattatattg
    10321 aaaatacaga tcctaaagca ggtatactga gtcaccaatt taagctcgat gacaataact
    10381 ttcttaatga tagatataaa gagtctatta aggcttcaac accatcaggt atgttctatg
    10441 aacgtaatat caacggtatg tgggtgtctg gtgacggtgt agtatatgcc gactttgatt
    10501 tgaatgagaa tacgattaaa gcagatgaac tggacgacat acctatcaaa gaatactttg
    10561 ctggtgtcga ctggggttac gagcactatg gatctattgt gttaatagga cgaggtatag
    10621 atggtaactt ttattttatt gaggagcacg cacaccaatt taagtttatt gatgattggg
    10681 tggttattgc aaaagatatt gtaagtagat atggcaatat taatttttac tgcgatactg
    10741 cacgacctga atacatcact gaatttagaa gacatagatt acgtgcaatt aacgctgata
    10801 aaagtaaact atcgggtgta gaggaagttg ctaagttgtt caaacaaaac aagttacttg
    10861 ttctttatga taatatggat aggtttaagc aagaggtatt taaatacgtt tggcacccta
    10921 caaacggaga gcctataaaa gaatttgatg acgtgttgga ctcgttaaga tatgccatat
    10981 acacacatac taaacctgaa cgattaagga gggggaatga cattgtataa gttaatagat
    11041 gatattgaag cacaaggaat attgcctaag catattgagg ctctaataga gtcacataaa
    11101 gacgatagag agagaatggt taatctctat aatagataca agacacatat tgactatgta
    11161 ccaatattca aacgtcgacc aattgaagaa aaagaagatt ttgaaactgg tggaaatgta
    11221 aggcgattag acgtgtctgt taataacaaa cttaacaact cttttgacag cgaaattgtt
    11281 gatacacgtg ttggttattt acatggtgtt cctgttactt atgatttaga tgaaaacgca
    11341 gaaaaaaacg aaaagttgaa aaagtttata accaactttg ccattagaaa tagtgttgat
    11401 gatgaggatt ctgaaatagg taaaatggca gcaatttgcg gatatggtgc taggttagca
    11461 tatattgata cgaatggtga tattaggatt aagaatatag atccctataa tgttattttt
    11521 gttggcgaca atattttaga acctacatac tcattgcgct acttttatga aaaagatgat
    11581 gataatggca ctgattatgt gtacgcagag ttttacgata atacttatta ttatgtattt
    11641 cgaggagaag gtattgacgc tttgcaagaa gttggacgat atgaacattt atttgattac
    11701 aatccattgt ttggtgtacc taacaacaaa gagatgatag gagatgctga aaaggttatt
    11761 cacttaattg acgcatatga tttaacaatg agcgatgcat caagtgagat tagtcagaca
    11821 cgtttagcat accttgtgtt acgcggtatg ggtatgagtg aagaaatgat tcaagaaaca
    11881 caaaagagtg gcgcatttga gttgtttgac aaagatatgg acgttaaata cttaacaaaa
    11941 gatgtaaatg acacaatgat tgagaaccat ttagatcgaa tcgaaaagaa tatcatgcgt
    12001 tttgcaaagt cagtaaactt taattctgac gagtttaacg gaaatgtacc tatcattgga
    12061 atgaaactta aacttatggc tttagagaac aagtgtatga cgtttgagcg taagatgaca
    12121 gctatgttga ggtatcaatt caaagttatt ttatctgcat taaagcgtaa agggtacaac
    12181 ttggatgatg atagttattt aaacctgata tttaagttca ctcgtaacat tccagttaat
    12241 aagttagaag aatcacaagt gctaattaac ctgaagggac aagtttcaga acgaacaagg
    12301 ttaggacaat cacaactagt tgatgatgtt gattacgaat tagacgaaat ggaaaaagaa
    12361 agtcttgaat ttaatgacaa attacctgac atagatgaag gtgacgcaaa tgacaaatcc
    12421 caaaataacc aatcagaatg atattgatga gtatatcgag ggtttaatct ctaaagcaga
    12481 aaaaccaata gaacaactat ttgctaatcg acttaaagag ataaaacaaa tcatcgcaga
    12541 tatgtttgag aaatatcaaa atgatgatgt gtatgttaca tggactgaat tcaataaata
    12601 caacaggctc aataaggagt taactcgtat aggtacaatg ttgactgatg actataggca
    12661 agtagctaag atgattcaga agtcacaaga agatgcttat atagaaaaat tccttatgag
    12721 cctttattta tatgaaatgg cgagtcaaac atctatgcag tttgatgttc cgagtaaaga
    12781 ggtaatcaaa tcagctattg aacaacctat tgagttcatt cgtttaatgc caacactaca
    12841 aaaacatcgt gatgaagtat tgaaaaagat acgtatgcac attacacaag gtattatgag
    12901 tggagagggt tactctaaga tagcaaaagc aatacgcgat gatatcggca tgtctaaagc
    12961 tcaatcattg cgtgtggctc gtacagaagc aggcagagca atgtcacaag ctggacttga
    13021 tagcgcaatg gttgctaaag ataacggttt gaagatgaag aaacgttggc atgctactaa
    13081 agatacacga acacgtgata ctcatcgtca tttagatggg gaatcagtgg aaatagatca
    13141 gaattttaaa tcaagtgggt gtgttgggca ggcgcccaag ctatttattg gtgtaaacag
    13201 tgcgaaagag aatattaatt gtcgttgcaa attactttat tatattgatg aaaatgaatt
    13261 gccaactgta atgagagcac gtaaagacga tggtaaaaat gaagttatcc cattcatgac
    13321 ttatcgtgag tgggagaaat ataagcgaaa aggtggtaat tgatatggat tttaaaataa
    13381 aagtaaatgt tgatactggc gaagctatag aaaagttaga acgcattaaa tccttgtacg
    13441 aagagataat agagttacaa aacgaaaaag ttggtgtaaa cgtaacagtt aaaaatgaag
    13501 ctgatttaga tatggttaaa acatctatta gcgaagaaaa tgctaaaaat aatgatttca
    13561 cactttttta gttgtctctt tgctactcga ccttagcatg tcgttaaact gctttttatt
    13621 atgcactttt cggactgtta gggtacgcga agggcaaaaa ggagttttga tatatgaata
    13681 tcgaagaagt taagtctttt tttgaagaac acaaagacga taaagaagta aaagattatc
    13741 taaacggact taagacggtg tctgttgatg acgttaaagg ctttttagat acagaagaag
    13801 gtaaacgatt cattcaacct gaattagatc gttatcattc gaaaggatta gaatcatgga
    13861 aagagaaaaa tcttgagaat ttaatcgaac aagaagtaca gaagcgtaat cctgagcaat
    13921 cagaagaaca aaaacgtatt agtgctcttg aaaaagagtt agaaaaacgc gacgcagagg
    13981 caaaacgtga gaagttaaga agtaacgcgc taggtaaagc gcaggaacta aatttaccaa
    14041 catccttagt tgatagattt ttaggcgatt ctgatgaaga tactgagcaa aacttaaaag
    14101 ctttaaaaga aacatttgac aagtatgttc aaaaaggtgt tgagtctaaa tttaaatcga
    14161 gtggaagaga tgttaaagaa tcacaaaatc aagatttaga cccttcaaat gtaaagtcca
    14221 ttgaagaaat ggcgaaagaa atcaatatta gaaaataaag tgaggtaata aaatatggca
    14281 actccaacat acacgccagg caatgttatt ttatcggatt ttaaaaacgg cgttattcca
    14341 gcagaacaag gtactttaat catgaaagac attatggcta attcagcaat tatgaaatta
    14401 gctaaaaatg agccaatgac agcacaaaag aaaaaattta cttacttagc aaaaggtgta
    14461 ggcgcctact gggtatcaga aacggaacgt attcaaactt ctaagcctga atatgcacaa
    14521 gcagaaatgg aagctaagaa aattggtgta attattccgt tatcaaaaga gtttcttaaa
    14581 tggactgcaa aagatttctt taatgaggtt aaacctctaa ttgcagaggc attttacaaa
    14641 gcgtttgacc aagctgttat ctttggtact aaatcacctt acaacacttc aactagtggt
    14701 aaaccgcttg ttgaaggcgc agaagagaaa ggtaacgttg ttacagatac taataattta
    14761 tacgtagacc tttcggcatt aatggctact attgaagatg aagagttaga tccaaacgga
    14821 gtattaacta cacgttcatt cagaagtaaa atgcgtaatg ctttagatgc taatgacaga
    14881 ccattatttg atgctaacgg gaacgagatt atgggattac cactatctta tactggagcg
    14941 gatgtatacg acaaaaagaa atcgttagca ctaatgggtg attgggatta cgcacgttac
    15001 ggtatcttac aaggtattga gtatgcaatt tctgaagatg ccacgttaac gacgttacaa
    15061 gcatcagatg cttctggcca accagtatca ttatttgaac gtgatatgtt cgctttacgt
    15121 gcgacgatgc atattgcata catgaacgtt aaaccagaag cgttcgcaac gcttaaacca
    15181 actgaatagg aggagatatg atggctaatc ctgcagaaga gattaaggta aaaaaagaca
    15241 atatgactat tactgttaca aagaaggcat ttgactctta ttacagtctt gtcggttaca
    15301 aagaggttaa atcacgtcgt actacgtctg ataagagcga gtgataaaaa tgactcttta
    15361 tgaagatgtt aaacttttac tcaagaaaaa tggagtggaa gttaaaagtg atgaagaaga
    15421 aatatttaag atggaagttg acggaatact agaagatgtt agggatataa caaacaatga
    15481 ttttatgaaa gatggtcaag tcatttatcc ttactcaatc aaaaagtatg tcgcagatgt
    15541 cctagagtat tatcaacgac ctgaagttaa aaagaattta aagtcaagaa gtatggggac
    15601 agtgtcgtac acttataacg atggtgtccc tgattacatt agtggagtat taaacaggta
    15661 taaacgagca aagtttcatc cgtttaaacc aataaggtag aggtgttgtt tgtgtttaac
    15721 ccatacgacg aattccctca cactatttct attggaagta tcaaaaaagt aggagagtat
    15781 ccaattatac aagagcgctt tgtaagcgat aaaacaatta aaggatttat ggatacgcct
    15841 actacatctg aacaactaaa atttcatcaa atgtcacaag aatatgacag aaacctatat
    15901 gtaccttatg acttgccaat atctaaaaac aatttatttg agtatgaggg tagaatcttt
    15961 agtattgaag gtgattctgt agatcagggc ggacaacatg aaattaagtt actacgactt
    16021 aagcaggtgc catatggcaa aagttaagta cggtgctgat agcatggttg ttgaattgga
    16081 taagttcgat aagaaaatag aagagtgggt taaaaaaggt attgctaaaa caacgacgaa
    16141 gatttacaac actgctgtag cattagctcc tgttgactta ggttttttag aagaaagtat
    16201 tgactttaaa tatttcgatg gtgggttatc cagtgttata agtgtcggcg cagattatgc
    16261 aatatacgtt gaatacggta ctggtatata tgctactggt cctggtggta gtcgcgctac
    16321 aaagattccg tggagtttta aaggtgatga cggcgaatgg tacaccacat atggtcaagc
    16381 gccacagcca ttttggaacc ctgcaattga cgcaggacgc aagacattcg agcagtattt
    16441 ttcatagagg tggttaaata tgtgggtatc agttgagcct gaacttacaa atcaaatata
    16501 taaaagatta atctcagacc ctaacattaa caaactagtt gatgataggg tctttgacgt
    16561 tgttcaagat gacgctgttt acccatatat tgttgtgggt gaatcaaacg tcactaacaa
    16621 cgaatctagc gcaacaatga gagaaacagt cggtattgtc atacatgtgt attcacagtt
    16681 cgctacacaa tacgaggcta agctcatttt aagcgcgata ggttatgtgc ttaacagacc
    16741 tatagaaata gataattacg agtttcaatt tagccgtatc gatagtcaag cagtattccc
    16801 tgatatagac aggtttacta agcatggcac gatacggctt ttatttaagt acagacataa
    16861 aaagaaaaac gaaggagtgt attaaatggc gcaaaaaaac tatttagcag ttgtacgtcc
    16921 agctgaaact gacttagatc cagtagaatc tttattatta gctgacttac aagaaggtgg
    16981 acatacgatt gaaaatgatt tagctgaaat agtacgaggc ggtaaaacgg actattctcc
    17041 caatgcaatg tcagaatcat ttaaattaac aattggtaat gtgcctggag ataaaggaat
    17101 tgaagcagtg aaacacgctg tacaaacagg tggacagttg cgtatatggc tttatgagcg
    17161 taataaacgt gcagacggta aacatcacgg aatgtttggt tatgttgttc cagaatcatt
    17221 tgaaatgtca tttgatgatg aaagtgacaa aatcgaacta tcattaaaag ttaaatggaa
    17281 tacagcagaa ggtgctgaag ataacttgcc gaaagagtgg tttgaagctg caggtgcgcc
    17341 tacagttgaa tacgaaaaat tcggcgaaaa agtcggaaca ttcgagaatc aaaagaaagc
    17401 tagtgttgta tctgattcac acacggaaga ccattctatg taaactaata gatcaagggg
    17461 gcgtaagctc cctatttttt tataaaaaaa ttgaaaagag gtatatattt tgactgaatt
    17521 taatccaatt acaacattaa aaattaatga cggagaaaaa gattacgaag tagaagcaaa
    17581 agtaacattt gcatttgacc gaaaagctga aaaattctca gaagatagcg aagatgggag
    17641 aaaaggagca atgccaggat tcaatgttat ctttaacggt ttgctagaat ctagaaacaa
    17701 agcgatttta caattttggg aatgtgctac tgcttattta aaaaacccac caactcgaga
    17761 acaattagaa aaagcaattg atgatttcat cactgaaaac gaggatactt tgccgttatt
    17821 acaaggggct ttggacaaac ttaacaatag tggttttttc aagagggaga gtcgctcgta
    17881 ctggatgaca ttgaacaaag caccgaatat ggccaaaagc gaggacaaag aaatgacgaa
    17941 agcaggcata gaaatgatga aagagaatta caaggaaatc atgggcgcag aaccttacac
    18001 gattactcaa aaataaggca actgacagct agatatttag gatatatccc tgaacatgaa
    18061 ttgttagcac taacacctgc tgaatggcgt gattggctta ttggtggtca ggataggtac
    18121 ctagatcaaa gacaattatt aattgaacaa gcgcaagcta acggcttagt acaagcttct
    18181 aagaggctaa ctagtatgat tcgtgacatt gagaaacaac gttacgaaat aagagaacct
    18241 ggtagctatg ctcgtgtaca aaaagctaga ttagaagaag aaaaaagaag acgtgaactc
    18301 ttcaaagaag gtacaagaaa attccttgaa tcgaaaggag gttagccttt ggatactcat
    18361 tttatggcaa agattatggc caatattaga gatttccaaa gcaacgtaag gaaagctcaa
    18421 cgattagcaa agacgtctgt accaaacgaa attgaaacag atgtaaaagc agatatttca
    18481 agattccaaa gagctttaca acgcgctaaa tcaatggctc aacgatggcg agagcattct
    18541 gttaaattat tcatgaaaac agatgagtat aaagcgaatt tagaacgcgc taaagctcaa
    18601 gtagagcgat ttaaacaaca taaagtagat ttgaaactaa gtaacactga attaatggcc
    18661 aaatataatg caactaaagc tactgtcgaa gcttggagaa aacatgttgt taagttggat
    18721 ttagatgcaa accccgctaa aatggcggtt aaagggttta aagaagattt aatagatctt
    18781 agcaggcata gttttgatat tgattccagc agatggaaat taggaaataa attcacaaaa
    18841 gaattcaatg aagtcgaagg agcagttaaa cgttctttcg gaagaattgg tcagattatg
    18901 agaaaagaag taaatggaac aagtgatatt tggggtaaac ttaacaactc attgaaagat
    18961 tacggcgaga aaatggacgc cttagctact aaaatccgaa ctttcggtac tatcttcgcg
    19021 caacaggtca aaggcttaat gattgctagt atacaagcat tgataccagt gattgccgga
    19081 ttagtacctg caataatggc agtacttaat gcggttggtg tattaggtgg tggcgtttta
    19141 ggtttagttg gcgcattctc tgtcgcaggt cttggagttg ttggctttgg tgcaatggct
    19201 attagcgctc ttaaaatggt tgaagatgga acattggcag taacaaaaga agttcaaaac
    19261 tttagagatg cgagcgatca gttaaaaact acatggcgtg atattgttaa agagaatcaa
    19321 gcaagtatct ttaatgcgat gtcagcaggt atcagaggtg ttacaagtgc gatgtctcaa
    19381 ttaaaaccat tcttatctga agtatctatg ctggttgaag caaacgcacg caagtttgag
    19441 gattgggtta aacattctga aacaactaag aaagcatttg aagcattgaa tagcataggt
    19501 ggcgcaatct tcggagattt attgaacgct gcaggaagat ttggcgacgg attaattaac
    19561 attttcactc aattaatgcc gttgttcaaa tttgtgtctc aaggactaca gaacatgtcc
    19621 atagctttcc aaaattgggc taatagtgtg gctggtcaga atgctattaa agcgtttatt
    19681 gactacacta ccactaactt acctaagatt ggtcagatat ttggcgatgt gttcgctggt
    19741 attggtaatt taatgattgc ttttgctcaa aacagttcta acatttttga ctggttagtt
    19801 aaattaactt ctcaatttag agcatggtca gaacaagtag gacaatcaca aggatttaaa
    19861 gactttatca gttacgttca agagaatggt cctactatta tgcagttaat cggtaatatc
    19921 gtaaaagcgt tagtggcatt tggtactgca atggctccta tagctagtaa attactagat
    19981 ttcgttacta atttagctgg atttatcgcc aaactattcg aagcacaccc agcagtcgct
    20041 caaattatcg gtgttatcgg tattttaggt ggcgtatttt gggctttaat ggctccgatc
    20101 gcagctgtta gcagtgtgtt aagtaatgtg tttagtatga ctttattgaa tgttgtcaaa
    20161 agaatactgg atttaactag aataactggg gtggtaagta aagcgttcgg tttattgact
    20221 ggtgctttca caagtatttc ttggccaata ttagcagtag ttgcagtcat tggtgtattc
    20281 attggtattc ttgtttattt atggaaaaca aacgagaatt tcagaaaaac aataacagaa
    20341 gcttggaacg gtattaaaac agcagtttcc ggtgcgattc aaggtgtagt agattggtta
    20401 actcaattgt ggggcaaaat tcaatcaaca ttacagccaa tcatgcctat attacaagta
    20461 ttaggacaag tattcatgca agttttaggt gttttggtaa taggcattat tacaaatgtt
    20521 atgaatatca tacaaggttt gtggacgtta attacaattg cgttccaagc cataggaaca
    20581 gtgatatccg tagctgtcca aatcatagta ggtttattca ctactttaat tcagttgctt
    20641 actggcgact tctcaggtgt ttgggagact attaaaacta cggttaccaa tgtacttgat
    20701 acgatttggc aatacatgca atcagtttgg gcgtcaatta tcggcttttt aactggcgta
    20761 atgaatcgaa cgctttcaat gtttggtaca agttggtcac agatatggag tacaatcact
    20821 aattttgtta gcagtatttg gagcactgtt acaagttggt tcagtcgtgt tgcttggagt
    20881 gtggctgaaa aaatgggaca agcattaaac tttattatca caaaaggttc tgaatgggtt
    20941 tctaacattt ggaatacagt tacaagtttc gcgagtaaag tagctgatgg gtttaaaaga
    21001 gttgtctcaa atgtaggtga cggtatgagt gatgcacttg gtaagattaa aagtttcttc
    21061 agtgatttct taaatgccgg agcggaatta atcggcaaag tagctgaggg tgtagccaat
    21121 gctgcgcaca aagtagtcag cgcggtaggc gatgcgattt catcagcttg ggactctgta
    21181 acttcattcg taagtggaca cggtggaggt agtagcttag gtaaaggttt agcggtatca
    21241 caagcaaaag taattgctac agactttggc agtgccttta ataaagagct atcctctact
    21301 ttgacagata gtatagtaaa tcctgtaagt acttctatag acagacacat gactagcgat
    21361 gttcaacata gcttaaaaga aaataataga cctattgtga atgtaacgat tagaaatgag
    21421 ggcgaccttg atttaattaa atcacgcatt gatgacatga acgctataga cggaagtttc
    21481 aacttattat aagggaggtt tgttagttga tagcgcacga tatagaagta ataaggaatg
    21541 gttcacagta tcgcgtcagt gacaatcctt tcacttataa tcacttggaa gtagttgaat
    21601 ataacgttac aggcgcagga tatcatcgta actattctga tatagagggt attgatggta
    21661 gatttcataa ttacgctaaa gaagaactta aaaaagtaga gcttaagata aggtataaag
    21721 tacctaaaat tgcttatgct tcacatttaa agtcagacgt ccaagcacta tttgctggac
    21781 gtttttattt aagggaatta gctacaccag acaattcaat taagtatgag catatattag
    21841 atataccaaa agacaaacaa gcatttgagc ttgattatgt tgatggacga caactttttg
    21901 taggactagt aagtgaagtt tcttttgaca caacacaaac atcaggggaa ttttctttgt
    21961 cgtttgaaac aaccgaacta ccatactttg aaagtgtcgg ttatagtact gatcttgaaa
    22021 gtaataacga ccctgaaaaa tggtcggtac ctgatagatt gcctacaaac gaaggtgata
    22081 agaggcgtca aatgacattt tacaacacta actcaggaga agtttattat aacggtgatg
    22141 ttcctttaac acagtttaat cagtttaatg ttgttgaaat agagttagct gaagatgtta
    22201 aagctaatga taaggatgga ttcactttct atacagataa aggaaatatc tcagttatta
    22261 aggaagttga tttaaaagcc ggagataaaa taatcttcga cggtaaacat acctatagag
    22321 gttatttaaa tatagattct tttaataaaa ctttagaaca accggtttta tatccaggct
    22381 ggaatcgatt caagtctaat aaagtaatga aacaaattac atttagacac aaattatatt
    22441 ttagataagg agtagcctat gccaatttta ttaaaaagtc tacagggtgt agggcacgct
    22501 attaatgtta gtacaaaggt aagtaaaaag ctaaatgaag atagttcttt ggatctaact
    22561 attatcgaga acgcgagtac gtttgacgca ataggtgcta taactaaaat gtggacgatc
    22621 actcatgttg aaggtgaaga tgatttcaac gaatatgtaa ttgtcatact tgataagtct
    22681 actattggcg aaaaaataag gcttgatatc aaagctaggc aaaaagaact tgatgacctt
    22741 aacaattcta ggatttacca agagtataac gaaagtttta caggcgttga gttcttcaat
    22801 actgtcttta aaggaacggg ttataagtat gtattacatc caaaagtaga tgcatctaaa
    22861 ttcgagggat taggcaaagg agatacacga ttagaaatct ttaaaaaagg acttgagcgt
    22921 tatcatctcg aatatgaata cgatgcaaag actaaaacgt ttcatttgta tgatgaatta
    22981 tctaagtttg ccaattatta cattaaagct ggtgtgaatg ctgataacgt caaaatacaa
    23041 gaagatgcat ctaaatgtta tacctttatt aaaggttatg gtgattttga tggacaacag
    23101 acttttgcag aagcgggact acaaattgaa ttcactcatc cattagcaca attgataggt
    23161 aaaagagaag cgccaccgct tgttgatgga cgtattaaaa aagaagatag tttaaaaaaa
    23221 gcaatggagt tattgataaa gaaaagtgtc actgcttcta tttccttaga ctttgtagcg
    23281 ttacgtgaac atttcccaga agctaaccct aaaataggtg atgttgttag agtggtggat
    23341 tctgccatag gatataacga cttagtgaga atagtcgaaa tcactacaca tagagatgcg
    23401 tacaataata tcactaagca agatgtagta ttaggagact ttacaaggcg taatcgttat
    23461 aacaaagcag ttcatgatgc tgcaaattat gttaaaagcg taaaatctac aaaatccgac
    23521 ccatctaaag aactaaaagc attaaacgca aaagttaacg caagtttatc tataaataat
    23581 gaattggtta agcagaatga aaaaataaac gctaaagtcg ataagatgaa tactaaaaca
    23641 gttacaactg ctaatggtac gatcatgtac gactttacta gtcaatcaag tataagaaac
    23701 atcaaatcaa ttggaacgat tggcgactct gtagctagag ggtcgcacgc aaaaactaat
    23761 ttcacagaaa tgttaggcaa gaaattgaaa gctaaaacga ctaatcttgc aagaggtggc
    23821 gcaacaatgg caacagttcc aataggtaaa gaagcggtag aaaacagcat ttatagacaa
    23881 gcagagcaaa taagaggaga cctaatcata ttacaaggca ctgatgatga ctggttacac
    23941 ggttattggg caggcgtacc gataggcact gataaaacgg atacaaaaac gttttacggt
    24001 gccttttgtt ctgcaattga agttattaga aagaataatc cagattcaaa aatactagtg
    24061 atgacagcta caagacaatg ccctatgagt ggtacaacaa tacgccgtaa agacacggac
    24121 aaaaacaaac tagggttaac acttgaggac tatgtaaacg ctcaaatatt agcttgtagt
    24181 gagttagatg taccagtgtt tgacgcatat cacacagatt actttaagcc atacaatcca
    24241 gcttttagga aagcgagcat ggaggacggc ttacacccta acgaaaaagg tcacgaggtt
    24301 attatgtacg agttaatcaa ggattattac agtttttacg actaaaggag gcaaccaatg
    24361 gcttacggat taattacaag tttacattca atgacaggtc ggaaaatagt tgctcaacat
    24421 gagtataact atcgcttgtt agatgaaggt atgagcaaac ttgagaaaat gtttatatac
    24481 catcaaaaag aagaaatata cgcacactca gcgaaacaaa ttaaatactt gaatgacagt
    24541 gttgaagatt atttaacgta tttaaatggc cgttttagca atatgattct aggccataac
    24601 ggcgacggta tcaatgaagt aaaagacgcg cgtattgata atacaggtta tggtcataag
    24661 acattgcaag atcgtttgta tcatgattat tcaacactag atgctttcac taaaaaggtt
    24721 gagaaagctg tagatgaaca ctataaagaa tatcgagcga cagaataccg attcgaacca
    24781 aaagagcaag aaccggaatt tatcactgat ttatcgccat atacaaatgc agtaatgcaa
    24841 tcattttggg tagaccctag aacgaaaatt atttatatga cgcaagctcg tccaggtaat
    24901 cattacatgt tatctagatt gaagcccaac ggacaattta ttgatagatt gcttgttaaa
    24961 aacggcggtc acggtacaca caatgcgtat agatacattg atggagaatt atggatttat
    25021 tcagctgtat tggacagtaa caaaaacaac aagtttgtac gtttccaata tagaactgga
    25081 gaaataactt atggtaatga aatgcaagat gtcatgccga atatatttaa cgacagatat
    25141 acgtcagcaa tttataatcc tatagaaaac ttaatggttt ttagacgtga atataaaact
    25201 tctgaacaac aagctaagaa cgcattaaat tttgttgaag taagaagtgc tgacgatatt
    25261 gataaaggta tagacaaagt actgtaccaa atggatatcc ctatgcaata cacatcaggt
    25321 acgcaaccta tgcaaggtat tgcttatgat gcaggtatct tatattggta cactggcgat
    25381 tcaaatccag ctaaccctaa ttacttacaa ggcttcgata tcaaaacgaa agaattgtta
    25441 tttaaacgtc gtatcgatat aggcggtgtg aataacaact ttaaaggaga tttccaagag
    25501 gctgagggtc tagatatgta ttacgatcta gaaacaggac gtaaagcact tttaatcggg
    25561 gtaactattg gacctggtaa caacagacat cattcaattt attctatcgg tcaaagaggt
    25621 gtaaaccaat tcttgaaaaa catcgcacct caagtatcaa tgactgattc aggcggacgt
    25681 gttaaaccgt taccaataca gaacccagca tatctaagtg atattacgga agttggtcat
    25741 tactatatct atacgcaaga cacacaaaat gcgttagatt tcccgttacc gagagcgttt
    25801 agagatgcag gttggttctt tgatgtactg cctggacact ataatggtgc tctaagacaa
    25861 gtacttacca gaaacagtac aggtagaaat atgcttaaat tcgaacgtgt cattgacatt
    25921 ttcaataaga aaaacaacgg agcatggaat ttctgtccgc aaaacgccgg ttattgggaa
    25981 catatcccta agagtattac aaaattatca gatttaaaaa tcgttggttt aggtttctat
    26041 atcactactg aagaatcaaa acgatttact gattttccta aagactttaa aggtattgca
    26101 ggttggatat tagaagtaaa atcgaataca ccaggtaaca caacacaagt attaagacgt
    26161 aataacttcc cgtctgcaca tcaattttta gttagaaact ttggtactgg tggcgttggt
    26221 aaatggagtt tattcgaggg aaaggtggtt gaataatggt agtagataat ttttcgaaag
    26281 acgataactt aatcgagtta caaacaacat cacaatataa tccaattatt gacacaaaca
    26341 tcagtttcta tgaatcagat agaggaactg gtgttttaaa ttttgcagta actaagaata
    26401 acagaccgtt atctataagt tctgaacacg ttaaaacatc tatcgtgtta aaaaccgatg
    26461 attataacgt agatagaggc gcttatattt cagacgaatt aacgatagta gacgcaatta
    26521 atgggcgttt gcagtatgtg ataccgaatg aatttttaaa acattcaggc aaggtgcatg
    26581 ctcaggcatt ctttacacaa aacgggagta ataatgttgt tgttgaacgt caatttagct
    26641 tcaatattga aaatgattta gttagtgggt ttgatggtat aacaaagctt gtttatatca
    26701 aatctattca agatactatc gaagctgtcg gtaaagactt taaccaatta aagcaaaata
    26761 tggctgatac acaaacgtta atagcaaaag tgaatgatag tgcgacaaaa ggcattcaac
    26821 aaatcgaaat caagcaaaac gaagctatac aagctattac tgcgacgcaa actagtgcaa
    26881 cacaagctgt tacagctgaa ttcgataaaa tagttgaaaa agagcaagcg atttttgaac
    26941 gtgttaacga agttgaacaa caaatcaatg gcgctgacct tgttaaaggt aattcaacaa
    27001 caaattggca aaagtctaaa attaccgacg attacggcaa agcaattgaa tcgtctgaac
    27061 agtccataga tagcgtttta agcgcaatta acacatctag gattattcat atcactagcg
    27121 caacagatgc gccaacattt aaagatatag gcactttaga gacgcctaaa gaagatggcg
    27181 ttgatgatgg ttctgaagtt tcagcaacta cgaatacttt agggaaatca ggcttgttag
    27241 ttgtttatgt tgttgatgac agtacagctc gtgctacatg gtatccagac gattcaaatg
    27301 atgagtacac aaaatataaa atcggtggca catggtatca gttctataaa aaagttgacg
    27361 aagaattaac gaagaaattt gttgaagaaa cgtctaacaa cgctttaaat caagctaagc
    27421 agtatgtaga tgataaattc ggaacaacga gctggcaaca acataagatg acagaggcga
    27481 atggtcaatc aattcaagtt aacttaaata atgcgcaagg cgatttggga tatttaactg
    27541 ctggtaatta ctatgcaaca agagtgccgg atttaccagg tagcgttgaa agttatgagg
    27601 gttatttatc ggtattcgtt aaagatgata caaacaagct atttaacttc acaccttata
    27661 actctaaaaa gatttacaca cgatcaatca caaacggcag acttgagcaa cagtggacag
    27721 ttcctaatga acataaatca acggtattgt tcgacggtgg cgcaaatggt gtaggtacaa
    27781 caatcaatct aactgaaccg tacacaaact attctatttt gttggtaagt ggaacttatc
    27841 caggtggcgt tattgaggga ttcggactaa ccgcattacc taacgcgatt caattgagta
    27901 aagcgaatgt agttgactca gacggcaacg gtggcggtat ttatgagtgc ttactatcca
    27961 aaacaagtag cactacttta agaatagata acgatgtgta ctttgattta ggtaaaacat
    28021 caggttctgg agcgaatgcc aacaaagtta ctataactaa aattatgggg tggaaataat
    28081 gaaaatcaca gtaaacgata aaaacgaagt tatcggattc gttaatactg gcggtttacg
    28141 caatagttta gatgtagatg ataacaatgt gcctattaaa tttaaagaag agttcgaacc
    28201 tagaaagttt gttttcacta acggcgaaat taaatacaat agcaatttcg aaaaagaaga
    28261 cgtaccgaat gcatcaaaac aacaaagtga atcagatttg agtgatgaag aacttcgcgg
    28321 aatggttgca agtatgcaaa tgcagatgac gcaagtgaac atgttgacaa tgcaattgac
    28381 gcaacaaaac gctatgttaa cacaacagtt gaccgaactg aaaactaaca aaacaaatac
    28441 tgagggggac gtttaaatga tgaagatgat ttatccaact tttaaagaca ttaaaacttt
    28501 ttatgtgtgg ggttgctata aaaatgagca aattaagtgg tacgtagaca tgggtgtaat
    28561 cgacaaagaa gaatatgcat tgatcactgg tgaaaaatat ccagaggcaa aagatgaaaa
    28621 gtcacaggtg taatgcttga ggctttttaa tttaacacaa agtaggtggc gtaatgtttg
    28681 gatttaccaa acggcacgaa catgaatggc gaattagaag attagaagag aatgataaaa
    28741 caatgcttag cactctcaat gagattaaat taggtcaaaa aactcaagag caagttaaca
    28801 ttaaattaga taaaacttta gatgctctcc agagggaaag acagatagac gaaaaaaata
    28861 agaaagaaaa cgacaaaaat atacgcgata tgaaaatgtg gattctcggt ttgataggga
    28921 ctatcttcag tacgattgtc atagctttac taagaactat ttttggtatt taaaggaggt
    28981 gattaccatg cttaaaggga ttttaggata tagcttctgg gcgtgcttct ggtttggtaa
    29041 atgtaaataa cagttaagag tcagtgcttc ggcactggct ttttattttg attgaaatga
    29101 ggtgcataca tgggattacc taatccgaaa aatagaaagc ccacagctag tgaagtggtt
    29161 gaatgggcgt tatatatcgc taaaaacaaa atagctattg atgtacctgg ttctggaatg
    29221 ggagcacaat gctgggattt acctaattat ttactcgata aatattgggg atttagaaca
    29281 tggggaaatg ctgatgctat ggctcagaaa tctaattata gaggtagaga tttcaagata
    29341 attagaaata caaaagactt tgtaccacaa ccaggcgact ggggtgtttg gactggtggt
    29401 tgggcaggac atgtaaacat tgtagtggga ccatgcacaa aagactattg gtatggcgta
    29461 gatcaaaact ggtatacaaa taacgcaaca ggaagtccac cttataaaat taaacactct
    29521 tatcatgatg gaccaggtgg aggggttaaa tattttgtta gaccaccata tcatccagac
    29581 aaaactacac cggcacctaa accagaagat gatagtgatg ataacgaaaa aaataataaa
    29641 aaagttccaa tttggaaaga tgtaacaact ataaagtaca ctatttctag ccaagaggtt
    29701 aattatccag aatatattta tcactttata gtagaaggta atcgacgact cgaaaaacct
    29761 aaaggaataa tgattagaaa cgcacaaacg atgagctcgg tagaaagttt atataacagt
    29821 aggaagaaat acaaacagga tgtagaatat ccccactttt atgttgatag acataatatt
    29881 tgggcaccta gaagagctgt atttgaagtt cctaatgaac ctgattatat agttatagac
    29941 gtatgtgaag attatagtgc gagtaaaaat gaatttattt ttaatgagat tcacgcaatg
    30001 gttgtagctg tagatatgat ggccaaatat gagatacctc taagtattga aaatttaaaa
    30061 gtagacgaca gcatttggcg ttctatgttg gaacatgtta attggaatat gattgacaac
    30121 ggtgttcccc ctaaagataa atacgaagca ttagaaaagg cattacttaa tatatttaaa
    30181 aacagagaaa aattattaaa ttctataact aaaccaacag taacaaaatc tagaataaaa
    30241 gttatggtag ataataaaaa cgctgatata gcgaatgtaa gagactcatc accaacagct
    30301 aataatggct cggcatctaa acaaccgcag attataactg aaacgagccc ttatacattc
    30361 aaacaagcac tggataaaca aatggcaaga ggtaacccga aaaaatctaa tgcttggggc
    30421 tgggctaacg ctacacgagc acaaacgagc tcggcaatga atgttaaacg aatatgggaa
    30481 agtaacacac aatgctacca aatgcttaat ttaggtaagt atcaaggtgt ttcagttagc
    30541 gcacttaata agatacttaa aggtaaggga acattgaata atcaaggtaa agcgttcgca
    30601 gaagcttgta aaaagcacaa cattaatgaa atttatttaa tcgcgcatgc tttcttagaa
    30661 agtggatatg gaacaagtaa cttcgctaac ggaaaagatg gagtatacaa ctacttcggc
    30721 attggcgctt acgacaacaa tcctaactac gcaatgacgt ttgcaaggaa taaaggttgg
    30781 acatctccag caaaagcaat catgggcggt gctagcttcg taagaaagga ttacatcaat
    30841 aaaggtcaaa acacattgta ccgaattaga tggaatccta agaatccagc tacccaccaa
    30901 tacgctactg ctatagagtg gtgccaacat caagcaagta caatcgctaa gttatataaa
    30961 caaatcggct taaaaggtat ctacttcaca agggataaat ataaataaag aggtgtgtaa
    31021 atgtacaaaa taaaagatgt tgaaacgaga ataaaaaatg atggtgttga cttaggtgac
    31081 attggctgtc gattttacac tgaagatgaa aatacagcat ctataagaat aggtatcaat
    31141 gacaaacaag gtcgtatcga tctaaaagca catggcttaa cacctagatt acatttgttt
    31201 atggaagatg gctctatatt caaaaatgag ccccttatta tcgacgatgt tgtaaaaggg
    31261 ttccttacct acaagatacc taaaaaggtt atcaaacacg ctggttatgt tcgctgtaag
    31321 ttgtttttag agaaagaaga agaaaaaata catgtcgcaa acttttcttt caatatcgtt
    31381 gatagtggta ttgaatctgc tgtagcaaaa gaaatcgatg ttaaattggt agatgatgct
    31441 attacgagaa tcttaaaaga taacgcgaca gatttattga gcaaagactt taaagagaaa
    31501 atagataaag atgttatttc ttacatcgaa aagaatgaaa gtagatttaa aggtgcgaaa
    31561 ggtgataaag gcgaaccggg acaacctggt gcaaaaggtg aagcaggtaa aaaaggagaa
    31621 caaggcgcac ccggtaaaaa cggtactgta gtatcaatca atcctggcac taaaatgtgg
    31681 caaattgatg gtaaagatac aaatatcaaa gcagaacctg agttattgga caaaatcaat
    31741 atcgcaaatg ttgaagggtt agaagataaa ttgcaagaag ttgaaaaaat caaagataca
    31801 actctcaacg actctaaaac gtatacggat tcaaaaattg ctgaactagt tgatagcgcg
    31861 cctgaatcta tgaatacatt aagagaatta gcagaagcaa tacaaaacaa ctctatttca
    31921 gaaagtgtat tgcaacagat tggctcaaaa gttagtacag aagattttga gaaattcaaa
    31981 gaaacactaa atgatttata tgctccaaaa aatcataatc atgacgagcg gtatgttttg
    32041 tcatctcaag cttttacaaa agaggaagca gataaacttt atcaacctat cggttcttcg
    32101 cagccgtcac tgaatatttg gacaggcagt gaaacagaat ataattattt gtatcaaaaa
    32161 gaccctaata cactttactt aattaagggg tgattttatg gaaggtaatt ttaaaaatgt
    32221 aaagaagctt atttacgaag gcgaagaata tacaaaagta tatgctggaa atatccaagt
    32281 atggaaaaag ccttcatctt ttgtaataaa acccttacct aaaaataaat atccggatag
    32341 catagaagaa tcaacagcaa aatggacaat aaacggagtt gaacctaata aaagttatca
    32401 ggtgacaata gaaaatgtac gtagcggtat aatgagggtt tcgcaaacta atttaggttc
    32461 aagtgattta ggaatatcag gagtcaatag cggagttgcg agtaaaaata tcaactttag
    32521 taatccttca gggatgttgt acgtcactat aagtgatgtt tattcaggat ctccgacatt
    32581 gaccattgaa taattttaaa cgactaattt tttagtcgtt ttttattttg gataaaagga
    32641 gcaaacaaat ggatattaac tggaaattga gatttaaaaa taaagcagta ttaacaggtt
    32701 tagttggtgc attgttgtta tttatcaaac aaatcacaga tttattcgga ttcgatttat
    32761 caactcaatt aaatcaagcc agcgcgatta taggtgctat cctcacgcta cttacaggga
    32821 ttggcgttat tactgaccca acgtcaaaag gcgtctcaga ttcatctata gcacagacat
    32881 atcaagcgcc tagagatagc aataaagaag aacaacaagt tacgtggaaa tcatcacaag
    32941 acagcagttt aacgccggaa ttaagcacga aagcaccaaa agaatatgat acatcacaac
    33001 ctttcacaga cgcctctaac gatgttggct ttgacgtgaa cgagtatcat cacggaggtg
    33061 gcgacaatgc aagcaaaatt gactaaaaaa gagtttatag agtggttgaa aacttctgag
    33121 ggaaaacaat ttaatatcga cctttggtat gcatttcaat gctttgatta tgctaatgct
    33181 ggttggaaag ctttgtttgg attactccta aaaggtgtag gcgcaaaaga tattccgttc
    33241 gctaacaact tcgacggatt agctactgta taccaaaata caccggactt cttagcgcaa
    33301 cctggcgaca tggttgtatt cggtagcaac tacggtgctg gatatgggca cgttgcatgg
    33361 gttatcgaag caactttaga ttatatcatt gtatatgagc agaattggct cggcggtggc
    33421 tggactgacg gaattgaaca acccggttgg ggttgggaaa aagttacaag acgacaacat
    33481 gcttatgatt tccctatgtg gtttatccgt ccgaatttta aaagtgagac agcgccacga
    33541 tcagttcaat ctcctacaca agcacctaaa aaagaaacag ctaagccaca acctaaagca
    33601 gtagaactta aaatcatcaa agatgtggtt aaaggttatg acctacctaa gcgtggtagt
    33661 aaccctaaag gtatagttat tcataacgac gcaggaagca aaggggcaac agcagaagcg
    33721 tatcgaaacg gattagttaa cgcaccttta tcaagattag aagcgggtat tgcgcatagt
    33781 tatgtatcag gtaacacagt gtggcaagct ttagatgaat cgcaagtagg ttggcatact
    33841 gctaaccaat taggcaataa atattattac ggtattgaag tgtgtcaatc aatgggagcg
    33901 gataatgcga cgtttttaaa aaatgaacag gcgactttcc aagaatgcgc tagattgttg
    33961 aaaaaatggg gattaccagc aaacagaaat acaatcagat tacacaacga attcacttca
    34021 acatcatgcc cacacagaag ctcagtattg cacactggtt ttgacccagt aactcgtggc
    34081 ctattgccgg aagataaaca attacaactt aaagactact ttatcaagca aattagagtg
    34141 tatatggacg gtaagatacc agttgccact gtctctaatg agtcaagcgc ttcaagtaat
    34201 acagttaaac cagttgcgag tgcatggaaa cgtaataaat atggtactta ctacatggaa
    34261 gaaagtgcta gattcacaaa cggtaatcaa ccaatcactg taagaaaaat aggaccattc
    34321 ttatcatgcc cggtagctta ccaattccaa cctggtggat attgtgatta tacagaagtg
    34381 atgttacaag atggtcatgt ttgggtagga tatacatggg aggggcaacg ttattacttg
    34441 cctattagaa catggaatgg ttctgcccca cctaatcaga tattaggtga cttatgggga
    34501 gaaatcagtt agaatgacat agtcatgtct atttaagcag gtgcgttaca tacctgcttt
    34561 ctatttacat ttaaagataa aatgtgctat tattttacta gaacttttta acatttctct
    34621 caagatttaa atgtagataa caggcaggta ctacggtact tgcctatttt tttgttataa
    34681 tgtaattaca ttaccagtaa ccaatctggc ttaaaaccac atttccggta gccaatccgg
    34741 ctatgcagag gacttacttg cgtaaagtag taagaagctg actgtatatt taaaccaccc
    34801 atactagttg ctgggtggtt tttatgttat aatataaatg tgaaatggtc attcttgaaa
    34861 tgactcggtc gctactggca cagactgttt aaagtgtcac cacaacatga actgagaatt
    34921 catatgacgt tgctgacgag cgacaaagct ctgtgttcct gaatgggagt aggtttgtgt
    34981 ggtggtacat aacaagtcgc tgaaatattt gcgacataat aaaacatatt atcggtttta
    35041 ttatgtgctt caggcacacc ttaaccaccc atactagtta ctgggtggtt gtttatatat
    35101 aacgcaagtt aaccaaaact aactctatct aataaaaagt atgaaaaatt tattcatatc
    35161 tatctaataa aaagtatgaa aaatttactc atatctattg cgtataaagt taaaagatat
    35221 tatagttaac tatgaagaaa gtcaactctc tattccgttc tttcttccta acttgcattc
    35281 gttcgtagtt agttcgtcaa gtaactatta atttagttat atacaatcag gagtgaattg
    35341 tatagcccgg cagaggccat atatctgact gttggtcccg caggagactt cttccttgcc
    35401 atcactcata tacataatcc ctacttacat taatgtttgt agggatattt tttaaggggt
    35461 gtactaggtg gggaacacaa cgtatttaaa aataaatagt gaaaacgatg ttgatttaca
    35521 agacatcttg aatgatttta ttaattgctt ttgcaaaggt tatgtggaaa ttaaaacgaa
    35581 atataaattg cttcccatct ttaaaataaa ttttcataaa aataatttac cccacttatt
    35641 aggtttgcat tacacacata aaaaagtgag cgctaaaaag atcattggaa gaatagctga
    35701 agggaaaatt acacacgaat ctataaaaaa acattatgaa tatagtaaca ttaaagatag
    35761 gcttatcaat tataattttt tgcataaatg ctttattgat aaagaaatca ggctatgcgt
    35821 tatagttcca aaaaattcaa ttaatccaca aaagattgat gtagctttta tagatgacaa
    35881 gaacagccaa gttatgatac tcgggttaag gaagtctaac aataatgatt tttatagtcc
    35941 ggcgactatg tacgttctgg gtaaaaacag ttcatatcga agaatgagaa gaacacatgt
    36001 tattagcata gaatggaaaa attaataaat tcgcctatcg gtgaatcagt atagatcgca
    36061 tcttaaatgg tgtgtttatt ttactccccc tacaaccaac aaaaccacac cacctattaa
    36121 tttaggagtg tggttatttt tgtcgcgcgt gtcaaatacg tgtcaattta gttctattta
    36181 tttagtgttt tttctgaagt taaatgcttt caaatagctt agttatagtt ttttcggtta
    36241 tatgacaaaa tgaaatttat ccctcactct ccgttatagc gcttaaaatg gttttaccca
    36301 ttttaagcgc tatttttaag gttttttgtc tattatctga gtcacttagt ttaaaatgtg
    36361 tttgttcaat tgttaatagg attgttaagt tttaaaatcg taatcaaaaa g
//
LOCUS       DATTQF010000011        36359 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.011, whole genome shotgun sequence.
ACCESSION   DATTQF010000011 DATTQF010000000
VERSION     DATTQF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36359
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.011"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(372..2780)
                     /locus_tag="VB241_000460"
     CDS             complement(372..2780)
                     /locus_tag="VB241_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP1243792.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNRDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGCPVVT
                     DYHGDDQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQITKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            complement(3020..3211)
                     /gene="cwrA"
                     /locus_tag="VB241_000461"
     CDS             complement(3020..3211)
                     /gene="cwrA"
                     /locus_tag="VB241_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HEP1243793.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            3450..4049
                     /locus_tag="VB241_000462"
     CDS             3450..4049
                     /locus_tag="VB241_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEP1243794.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDKN
                     KRKELIDELFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPINIGSNTWFGGHVAVLPGVTIGEGSVVGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNEVPSEALNDETLN"
     gene            4239..5783
                     /gene="pruA"
                     /locus_tag="VB241_000463"
     CDS             4239..5783
                     /gene="pruA"
                     /locus_tag="VB241_000463"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEP1243795.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            5895..6062
                     /locus_tag="VB241_000464"
     CDS             5895..6062
                     /locus_tag="VB241_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243796.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFMFFFIVYVLYISYF
                     NSDKHDKNKKR"
     gene            complement(6139..6687)
                     /gene="farR"
                     /locus_tag="VB241_000465"
     CDS             complement(6139..6687)
                     /gene="farR"
                     /locus_tag="VB241_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HEP1243797.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHKVINIKIK"
     gene            6832..9321
                     /gene="farE"
                     /locus_tag="VB241_000466"
     CDS             6832..9321
                     /gene="farE"
                     /locus_tag="VB241_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HEP1243798.1"
                     /translation="MAKFLYKMGTFIAKYKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPSITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQINKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAVGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKHI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYNKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTFV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDARSIVLDDKTMALYQEVKQQSASSLFLYDALMDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            9536..9763
                     /locus_tag="VB241_000467"
     CDS             9536..9763
                     /locus_tag="VB241_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEP1243799.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            9775..11769
                     /gene="feoB"
                     /locus_tag="VB241_000468"
     CDS             9775..11769
                     /gene="feoB"
                     /locus_tag="VB241_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEP1243800.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            11782..11952
                     /locus_tag="VB241_000469"
     CDS             11782..11952
                     /locus_tag="VB241_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEP1243801.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDIDRDCCG
                     TEQHTANHFPGK"
     gene            complement(12022..14127)
                     /gene="clpL"
                     /locus_tag="VB241_000470"
     CDS             complement(12022..14127)
                     /gene="clpL"
                     /locus_tag="VB241_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HEP1243802.1"
                     /translation="MNNNFFNSDFDSILRRMMQDMQNSNQTSNKKYYINGKEVSPEEL
                     AQFTQQGGNHSAEQSAQAFQQAALRQQGQQSGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISI
                     DISSLEAGTQYRGAFEENIQKLVEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNIMKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFETHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAIDKVT
                     TEKRISELEHDKRKAVSAEEYKKADDIQKEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDANDIERLKNISSRLRSKIIGQDKAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKEALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDTEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            14490..15011
                     /locus_tag="VB241_000471"
     CDS             14490..15011
                     /locus_tag="VB241_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEP1243803.1"
                     /translation="MVYKSYYDSPVGRLELVSDGVSLTAVLFENQQDDGTREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KLLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            complement(15182..16348)
                     /locus_tag="VB241_000472"
     CDS             complement(15182..16348)
                     /locus_tag="VB241_000472"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP1243804.1"
                     /translation="MTLGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VISHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEQQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            16603..17883
                     /locus_tag="VB241_000473"
     CDS             16603..17883
                     /locus_tag="VB241_000473"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP1243805.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEEQFNILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHQIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASKDGQYRGIATWRYDQERQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQDLRSQQ"
     gene            complement(18023..18454)
                     /locus_tag="VB241_000474"
     CDS             complement(18023..18454)
                     /locus_tag="VB241_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1243806.1"
                     /translation="MKYKKILFRLLIAFAVLFSADFIYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(18842..19060)
                     /locus_tag="VB241_000475"
     CDS             complement(18842..19060)
                     /locus_tag="VB241_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HEP1243807.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            19227..20105
                     /locus_tag="VB241_000476"
     CDS             19227..20105
                     /locus_tag="VB241_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1243808.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            20318..20713
                     /gene="cidA"
                     /locus_tag="VB241_000477"
     CDS             20318..20713
                     /gene="cidA"
                     /locus_tag="VB241_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HEP1243809.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKQRKGIDAYE"
     gene            20706..21395
                     /locus_tag="VB241_000478"
     CDS             20706..21395
                     /locus_tag="VB241_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEP1243810.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLIILFY"
     gene            21435..23174
                     /locus_tag="VB241_000479"
     CDS             21435..23174
                     /locus_tag="VB241_000479"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEP1243811.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            23474..25540
                     /locus_tag="VB241_000480"
     CDS             23474..25540
                     /locus_tag="VB241_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HEP1243812.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKIVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            25920..26330
                     /locus_tag="VB241_000481"
     CDS             25920..26330
                     /locus_tag="VB241_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEP1243813.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            26372..26728
                     /locus_tag="VB241_000482"
     CDS             26372..26728
                     /locus_tag="VB241_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1243814.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            26849..27817
                     /locus_tag="VB241_000483"
     CDS             26849..27817
                     /locus_tag="VB241_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1243815.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTHYQFKMPVKVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQYYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVNGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(27898..28833)
                     /locus_tag="VB241_000484"
     CDS             complement(27898..28833)
                     /locus_tag="VB241_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEP1243816.1"
                     /translation="MRYLVNNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(29017..29220)
                     /locus_tag="VB241_000485"
     CDS             complement(29017..29220)
                     /locus_tag="VB241_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243817.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            29770..30813
                     /locus_tag="VB241_000486"
     CDS             29770..30813
                     /locus_tag="VB241_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP1243818.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            30816..31496
                     /gene="sdaAB"
                     /locus_tag="VB241_000487"
     CDS             30816..31496
                     /gene="sdaAB"
                     /locus_tag="VB241_000487"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP1243819.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIVEEE
                     GDSIGQHPNCAYIKSFRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIMIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            31510..32409
                     /gene="sdaAA"
                     /locus_tag="VB241_000488"
     CDS             31510..32409
                     /gene="sdaAA"
                     /locus_tag="VB241_000488"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP1243820.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            32428..32526
                     /locus_tag="VB241_000489"
     CDS             32428..32526
                     /locus_tag="VB241_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243821.1"
                     /translation="MIKKKHLSGSLVPDRCFLIIFHKVEKFMGNAC"
     gene            32588..32758
                     /locus_tag="VB241_000490"
     CDS             32588..32758
                     /locus_tag="VB241_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243822.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(33208..33699)
                     /locus_tag="VB241_000491"
     CDS             complement(33208..33699)
                     /locus_tag="VB241_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1243823.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
     gene            33893..34513
                     /gene="srtA"
                     /locus_tag="VB241_000492"
     CDS             33893..34513
                     /gene="srtA"
                     /locus_tag="VB241_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HEP1243824.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            34743..35543
                     /locus_tag="VB241_000493"
     CDS             34743..35543
                     /locus_tag="VB241_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1243825.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEANFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(35754..36234)
                     /locus_tag="VB241_000494"
                     /pseudo
     CDS             complement(35754..36234)
                     /locus_tag="VB241_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        12283 a         5599 c         6901 g        11576 t
ORIGIN      
        1 taataatatt gtagagtctg agacataaat caatgttcaa tgctctacaa agttatattg
       61 gcagtagttg actgaacgaa aatacgcttg taacaagctt ttttcaactc tagtcaacct
      121 tgccggcggg gccccaacat agagaaattg gattcccaat ttctacagac aatgcaagtt
      181 ggggtgggac gacgaaataa attttacgaa aatatcattt ctgtcccact cccaagatac
      241 actaattcgc caagcgaaaa gtgtaactat atatgcagtt ggagcgaaga tccaactgta
      301 aaccgcgaca ttatagagcc aatcactagc ctatctcaga atcaccaacg aatccttcat
      361 tattaaggaa tctaggcatc tttacggcgt ggttctaatc gcatcttttt caatctaagt
      421 gcgtttgtga caacacttac tgaacttagt gccattgcag caccagcaac ccatggcgca
      481 agtaagccta atgcagctat agggataccg gcaatattat agccgaatgc ccaaaataga
      541 ttttgacgaa tattacgaat ggttgcttta cttgcataaa tggctttagg aataagcatc
      601 aagtcgccac caagaatagt aatatcagcc gcttcaatag caacttctgt acctgtacca
      661 atggcgatac cgatatcagc tttaactaat gcaggtgcat catttacacc gtcaccaacc
      721 atcgcaacct tcttaccttg ttgctgtagt ttcgttattt gtgcagcttt ttcttctggt
      781 aaaatatctg caataacagt atctatgcct acttgctttg caatggcttg agcagtgttt
      841 ttattatcgc cagttaacat ggcaacttca atgcccatat catgcaattg ttttatagca
      901 tctttggcat gatcttttac agtatctgcc actgcgatga taccagttaa tgaataatta
      961 acagcaatga gcatagcagt tttaccatct cgttcataat gtgttaaatc atcagaaata
     1021 tgcttaggca agctaatatc attgtcagcc attaatttac ggttaccaac caatatatgg
     1081 tgatgatcaa tcgttgcttc aataccatgg ccaggtactg ctttaaatgt tgttgtctca
     1141 gttaatgtta attgcttttc ttttgcataa ttgacaatgg cttctgccaa tgggtgttca
     1201 gaatcttttt cagcagtagc aagtagttgt agcgtttgat cgtcaccatg ataatctgtc
     1261 acgactggac aaccatttgt aatggtaccc gtcttatcta aaacgatggt atcaatttga
     1321 tgtgtgcgtt caacaaactc gccaccttta aataaaatac cattttcagc agcgcgacca
     1381 gtacctacca taatagaagt tggtgtagca agtcctaatg cgcatggaca agcaatgacg
     1441 agaacggaaa tactcgcaac aagtgcaggt tcaaatgtac ctggtgtaac taaagtaatc
     1501 cacacgataa atgttaatag tgcgatacca acaacgatag gaacgaaata accagaaata
     1561 atatctgcca atcgctgaat cggcgcttta gaactttgag cttcttcgac aactttaata
     1621 atatttgcca atgcagtgtc cccgccaact tttgttgctg tcatagtaat agtaccgttt
     1681 ttgttcatcg ttgaaccaat tacagtatca tcaacattct tctcaacagg gatagattca
     1741 cctgttaaca tagattcgtc gatggcagtc atacctttaa taattttgcc atcaacaggt
     1801 atcttttcac ctggtttaac gataagtgta tctccaacat gtacctcatt tagaggaatc
     1861 atcacttcat taccgtcttt taaaatgcgt gcttctttag cttgtaaact taataattcg
     1921 ccaagcgcat tggttgtttg agacttcgct ctagcttcta aatacttacc gaataagatt
     1981 aaggtaatta gtacggcgct tgtttcaaag tataaatgcg gttgtgttgt tgagccattt
     2041 agccaacgaa ccatttcata aatactgtaa aaatatgctg cacttgtacc aacagcaaca
     2101 agtacatcca tattggcgcc accatttctt aaatttttat aagcacctac ataaaattgc
     2161 catccaataa taaattgtac aggtgtagct aaaataaatt ggaaccatgg attcgtaaat
     2221 agtgctggta tatgcatatt gaaaagatgt acaaacatta acattaatag cggtaaagat
     2281 aatactgctg atatgataag cttaatcaat ttatgttgta gcgcttcagc tttgcgtgac
     2341 gtttgatctc tattattatc tttaatagat gcgtcataac ctaatttttg aatgcgagtg
     2401 acaagtttat cagcatctgt ttcttcagga taatagtcaa ctttagcttg ctccgttgtt
     2461 aaattgaccg ttgcattttg aacgccgtcc attttattta acactttttc aatacggctt
     2521 gagcatgcag cacaagtcat gcctgtaatg tctaattcga cagtttctac agcgacaccg
     2581 taacctaaat gttgaatcgt attaataaat tcttggacat catgttgatc agggttatac
     2641 tcaacagttg ctttctctgt agttaaattc acttgggcat taacgtcatc aagtttattc
     2701 agtttctttt cgatacgatt tgaacatgcg gcacaagtca taccagtgat atctaatgtt
     2761 gtttttttcg tattagccac attattgcct ccctgtgatt cattgttaca cgtctaatgt
     2821 accccctata ggtatataag tcaatcgttg cgttttactt ttaccaattt taaaaagaaa
     2881 aagagagatt gccgtgcaaa cattgcacaa caatctctct atttagcatc tatataaaag
     2941 aagtcgtaat taatattaaa ggatgtatga atgaaaggta aaacttaaat gacttctgga
     3001 tttgtaattc ttaggtgact taaaagaaat cagatgggtt aaattctttc gcatgtaaat
     3061 ttttgaaatt attaagatgg atgactttat cgtcatcttt gttgtctgac aactgacgac
     3121 ttgtgtcgtt tgtcatttcg taatcttgta tcgttttaac taaacctaga atgattaaga
     3181 tagcaactgt gcctgtaatt aatattctca tcgctatcat actcctttat atttctctta
     3241 ctcacatttt acgttaatag attaaaaaat gaatgcgtct gtagtattga atttatctac
     3301 tactttagca gtatttgatg cgagaatggt atagataaga gtgataaggt tatgatttca
     3361 taatgttata gcttattagt tgcataggcg aatgtttatt tgattaacta ttaaacaagt
     3421 aaataagtta ttaggagttg aaaggtgtaa tgactgaaaa agaaaaaatg ttagcagaaa
     3481 aatggtacga tgcaaacttt gatcaagact taatcaatga acgtgcacga gcgaaagata
     3541 tttgctttga attaaatcat acaaagccga gtgacaaaaa taaaagaaag gaattaatcg
     3601 atgaattatt tcaaacaaca acagacaatg taagtatttc gattcctttt gatacagatt
     3661 atggttggaa cgttaaacta ggaaaaaatg tctatgtaaa caccaattgt tattttatgg
     3721 atggtggaca gattacaatt ggcgataatg tttttatagg acctaattgt ggattctaca
     3781 cagcaacaca tccacttaat tttcatcata gaaatgaagg atttgaaaaa gcaggaccaa
     3841 ttaatattgg cagtaatact tggtttggcg gacatgtagc cgtgcttccg ggagtgacga
     3901 ttggagaagg cagtgtggtt ggtgctggta gtgttgtcac caaagatatt ccgccacaca
     3961 gtttagcggt tggaaaccct tgtaaagtcg ttcgtaaaat tgataatgag gtaccatcag
     4021 aagcattgaa cgatgaaaca ctaaattagt tgtcatgaca cttctaaatt ttaaaaagta
     4081 accaatcaat ttcaaaatac ttgagaataa gggataaagc ataaaaatga aagcgattgc
     4141 aaaaccaacc ttactcatag taaaatgagt ttaaatgatg ttatatcatt aaatcaaagg
     4201 gttaaaccac tttcaaaatt tatagggggt aagtgtacat ggtagtagaa tttaaaaatg
     4261 aacctggtta cgatttttca gtccaagaaa atgtggatat gtttaagaaa gcattgaaag
     4321 acgtggaaaa agaattaggt caagacatac cattagtgat taatggagag aagattttca
     4381 aagatgacaa aattaaatca atcaatcctg cagacacatc acaagtgatt gcaaatgcat
     4441 caaaggcaac aaaacaagat gttgaagatg catttaaagc agcgaacgaa gcttataaat
     4501 cttggaaaac atggtcagca aatgatcgtg cagagcttat gttacgtgtt tcggcaatta
     4561 ttcgtcgtcg aaaagcggaa attgcagcaa ttatggttta tgaagcaggt aagccatggg
     4621 atgaagcagt tggtgatgca gctgaaggta ttgattttat tgaatactat gcacgttcaa
     4681 tgatggactt ggctcaaggc aagccagtac ttgatcgtga aggtgaacac aataaatatt
     4741 tctataaatc aattggtaca ggtgttacaa ttccaccgtg gaacttcccg tttgcgatta
     4801 tggctggtac aactttagca ccagttgtgg caggtaatac agtgttattg aaacctgctg
     4861 aggatacacc ttatatcgct tataaattaa tggaaatttt agaagaagca ggattaccaa
     4921 aaggcgtagt taactttgtt cctggtgatc cgaaagaaat tggtgattac ttagttgatc
     4981 ataaagatac gcattttgtt acatttacag gatcacgtgc gacaggtact agaatttatg
     5041 aacgtagtgc cgttgttcaa gaaggtcaaa acttcttgaa acgtgttatc gctgaaatgg
     5101 gcggtaaaga tgccattgta gttgatgaaa atattgatac agatatggca gcagaagcta
     5161 ttgtaacgtc agcgtttggt ttctctggtc aaaaatgttc agcatgttca cgtgcaattg
     5221 tgcataaaga cgtttatgac gaagtattag aaaaatcaat taaactaaca aaagaattaa
     5281 cgttgggtaa tactgtagac aatacgtata tgggcccagt tattaataaa aaacaatttg
     5341 ataagattaa aaattatatt gaaattggta aagaagaagg taaattagaa caaggtggcg
     5401 gcactgatga ttcaaaaggt tactttgtag aaccaacaat catttcagga cttaaatcta
     5461 aagatagaat catgcaagaa gaaatttttg gtccagttgt tggctttgtg aaagttaacg
     5521 attttgatga agcaattgag gttgcaaatg atacagatta cggtttaact ggtgcagtga
     5581 ttacaaataa tcgcgagcat tggattaaag cggtaaacga atttgatgta ggtaatttat
     5641 accttaacag aggctgtact tcagcagttg ttggatacca tccatttggt ggtttcaaaa
     5701 tgtcaggtac ggacgctaaa acaggtagcc cagactactt attacacttc ttagaacaaa
     5761 aggttgtttc agaaatgttc taatgcttca taatgaacaa gggccatcca ttgcggttgg
     5821 ctctttttat ttataacttt aaaaattaag taatagcaac catattttta gaaaaggatt
     5881 ctaggtataa gaatgtgaga tacgttattt caataataat gggaattgta ttagcaattt
     5941 ggtcatttaa acaactgagt caaagtcatt tagatagcgg atttatgttt ttcttcatag
     6001 tttatgtgtt gtatatcagt tattttaata gcgataagca cgataaaaat aaaaaacgat
     6061 agtagtacac gaattcatta acgtgtacac tatcgttttt attatgaatg ttggtaacgc
     6121 ttatgagcct ctaatttact atttaatctt aatattaatg accttatgga aaatgttatc
     6181 aatatcttcg ccaggccagt caattttttc atcttttatc caatgtataa aaccttcaat
     6241 caacgagtca taaatataaa atatgagatt atctgggatg tcgctgtcaa tatcgatacg
     6301 gtctctatta tttttgatat cgttatgcat aattttaatg cagacatttt ttaatacttt
     6361 attaaattca gcgtcttctt cttggaattc tgcgatttct ctcaaatcct ctttattatt
     6421 aatcgtatca ctcatcgttt ggaatggatg attaagacgg tcactgatat ctctagcaaa
     6481 gtagtcttta gtcaattgat tgaacaaata ctctagaaga tcatatttat cataaaagtg
     6541 tttataaaat gttgtgcggt gtacgagtgc gttgtcgcaa atttgattga cagtaatcgt
     6601 ttggaataat tgctgttcta acaattgtag caagctactc gacaatgctt tttttgtctt
     6661 tataactcgt aaatcagtct ctttcatcta catttctcct ttgtgtgtag tttaatatac
     6721 aaaattttcc aattgattct ttaagaggtc ggtgatagat actataatca aacaataatc
     6781 tacactgtat agttaaacta caacgttgat tttaaaagga ggcaatatac tttggcaaaa
     6841 tttttatata agatggggac attcatcgca aaatataaat ggtccgcggt cattgcatgg
     6901 atagtgattg tagctgcaat attgatacca cttgctacaa atgcaccgaa atttgacaat
     6961 gatattaaaa tgacaggact agagtcgtta gatacgaata aaaagattga aaaacatttt
     7021 aatcaagata gtgaaaaggc acaaatacgt gttgtcttta agacgactaa agatgatggt
     7081 attgtgcaac cgagtattac taaagatatt aagaaaacgt tagatgatat taaaaaagat
     7141 gacaagcaca ttgataaaat atcagatcct tatgaaaata agcaaattaa taaagataaa
     7201 acaactgcat ttgcagatat cacatatgac gttagtcaaa catctttaaa agatggctct
     7261 agagataatg ttaaaagtca tttgaaagac ttacgtgaca atcataatgt acaaacagag
     7321 ttaactggta caggaatgac atctacagaa gtaggcggta attctgaact agttggtatt
     7381 attgttgcat ttgttgtact gttaatcaca ttcggttcag ttattgctgc aggattacca
     7441 attataagtg cattaattgg tttagcttct ggtgtaggta ttatcagttt gttaacttat
     7501 gcattcgata taccaaatgt cacactgaca ttagccgtaa tgattggact agcagttggt
     7561 atcgattatg cattatttat tttattcaga tatcgacaag ttatgaaaac tgaaacagat
     7621 tacattaaag gcattggtct tgcagtggga acagcaggta gcgcagtcat cttcgctggt
     7681 gtgacagttg tgattgccgt atgcggttta tcgctagttg gtatagattt cttagcagtg
     7741 atggggtttg cttcggcaat tagtgttata tttgctgtat tcagtgcact gactttatta
     7801 cctgcgttaa ttagtatttt ccataaacac attaaagtga acaaattgca gtctaatttt
     7861 aaaaaagata ttgatacgcc gtggtctaaa tttattactg gtaatgcact tgcagcagta
     7921 ttattaggtt taattatatt agttgcagcg gctattccag ttagtcatat gcgtcttggc
     7981 attccggatg atggcgtaaa gccagcagat tctactcaga aaaaggcata cgatattatt
     8041 tcagataagt ttggcgaagg atttaatggc caaataccga tgctgattaa cgttaaagat
     8101 aaaaaagatg atccacaagg attacaacaa gatttacaat ctgtgtataa agatattaaa
     8161 gataagaaaa atgtggatat tgttacgccg ccacaaatga gtaaagataa tgattacgct
     8221 ttgatggtcg tgatacctaa gcaagggcct aatgcagaaa gtacaaatga cttagtgcat
     8281 gatttacgag attataataa agatgcgcaa gataaatatg gatttaaaac ggaaatttca
     8341 ggacaaagtg tcattaatat tgatatgtct aaaaagttaa atgaggcgat acctttattt
     8401 gcaacagtaa ttgttgtatt ggcatttttc ttattaatga ttgtcttccg ttcaatctta
     8461 ataccgttaa aagcagtttt aggatttgtc ttgtcattaa tggcaacact aggttttaca
     8521 acctttgtga tgcaagatgg ctttatgaaa ggattattcg gtatagaaac gacaggacca
     8581 atgctagcct tcttgccggt tattacaatt ggtatactat tcggcttagc aatggattac
     8641 gaagtcttcc ttatgtcgcg aattcatgaa gaatatagta aaacagggga taatgattat
     8701 tctatcaaag taggattgaa agaaagtggt cctgttattg tagcagctgc attaatcatg
     8761 tttagtgtct tctttgcgtt tgtattccaa gaagatgtca tgattaaatc aatgggtatg
     8821 gcattagcgt ttggtgtact ctttgatgcc ttcgtagtca gaatgatgtt aattcctgca
     8881 ttaactaaat tgtttggaaa aggttcgtgg tatttaccag catggttgaa tcgtattatt
     8941 ccacgagttg atattgaagg acatgcactt gaaaaatata aaacggttga atcacaggaa
     9001 tctgaagcta aagatagtaa ggaaacatac gacacaacct ttaaagtata tccacaaggt
     9061 gcaacaaatg tatcgaaaca tcaagatgta catggacaag atgatgcacg ttcaatagta
     9121 ctagatgata aaacgatggc attgtaccaa gaagtgaaac agcaatcagc aagttcattg
     9181 ttcttatatg atgctttaat ggattatcaa aacaagcatc aactaaattc aaaacaacaa
     9241 gccacgaata tagaacagtt gaataaaaat attgaaaagc tcaatcaatt gttagaaaaa
     9301 aacttaagaa ataaatcata aaataacatg tacatgcctc cgctttaaat gtctatcaat
     9361 gacatttgaa gtgggggttt ttgcatggaa gcgattatac aaaacatttt tgcaatgtca
     9421 caaattttac atgaaaaatg aaaattattt aaataaagta ttgcgagcta caattttaag
     9481 ggttatgatt tttaagtgat aatgattatt atttctaata ggagagcgaa atagtatgtt
     9541 aaacattaaa aatggtgaaa tgaataaggc ttataaaata aagcgaatgg atattgctaa
     9601 tgagaatatg ttgtatcgtc taagtgcctt tgggttaaca gatgacgcta tcataacgat
     9661 taaacaaaaa tgtttattta aagggccatg tattattgaa gtaaacggac aacagttaag
     9721 tattagacat tgcgatgctt gttctattgc attagaagaa taggagccgg acgaatggaa
     9781 aattattgta ttttaggaaa tcctaatgtt ggtaaaacat ctttgtttaa cgctttaact
     9841 ggttcgtatg aatatatagg caactggagt ggcgtaacgg tagagaaaaa agtagggaag
     9901 cttaaagaaa atgtaggaca attaattgat ttaccaggta catatgattt atcgccgatt
     9961 tctaaagatg aaactgtagt gacagactat ttattaaacg attctttttc aggaatcatt
    10021 aacattgttg atgcgagtca actaaaaaga aatatgcaat taacagtaca attgttagaa
    10081 cttaatcaac caatatacat cggattaaat atgattgatg tagctacaaa gcgcgggata
    10141 aaaatcgatt atcataaatt gatgaaaaag ttaaaaacac ctatttttcc agttgtggca
    10201 cgtacaggca aaggaactaa acatttactt ggcgaaataa agcatctagg ggaagggtat
    10261 caaccgcatt ttaaaatcaa ttatggtgag aagattgaag agacaattaa aaatatgtgt
    10321 caaataataa tgacagagac atctcatgat aaatatcaag ctagatttat tgcgattcaa
    10381 ttcttactaa ataacatgca aattgcaaat gaacttaatt cagaggttgt aaataaattg
    10441 tcgagtttac gtgatcaagt tgctgaacaa gtagaggctg tatctgttcg tcgagagatg
    10501 gaacgtatac gcaatcatta tattgagacg ttattgcaag acgtggtaac gtatccagat
    10561 gaagataagc aatatttcag ctctagaata gacaagatat taacacataa atatataggg
    10621 atgcctattt ttctagcgat tatgtggttg atctttcaaa ctacatttac atggattggc
    10681 acacctttgt cggatcaact cgatgcattt ataggtggta catttacgga tagtgtgaag
    10741 acgattatga attatcttgg cgttatacca tttttacaag atttaattac agatggcatt
    10801 attgctggtg taggatcagt attagtcttt gtaccacaaa ttgttgtgct ctttttcttt
    10861 atatctttat tagaagattc aggttatatg gcacgtattg cggttttaat ggatcgtatt
    10921 atggaatcgt tcggtttaag cgggaagtct tttataccga tgattatcgg atttggttgt
    10981 aacgtaccga gtattatggc tgcgcgcagt attgaaaatg aaaaagagcg tttaacaacg
    11041 atattaattg caccattcat gtcatgctct gctagactgc cagtgtatgc attgttcgta
    11101 ggtatctttt tcaaagaaaa tcaatcttta gtagtattaa gcttgtatgt tttaggtatt
    11161 attatggcat ttttagtcag tacagtactt actaagacag ttttaaaaaa tgataatgca
    11221 atctttattg tggagttgcc aacataccgt gtcccttcca ttaaaacgtt gtggcgtagt
    11281 acttgggaaa aggctaaggg ctttgttcgt aaagcaggta catttatttt tggcggctca
    11341 gtagtcattt ggctattaag ttatgtagga ccacatggta tcaatgtgaa tatcaatcaa
    11401 agttttttgc atatggttgg tagcttcttt ggtatgttag tgcaaccgct cggatttgga
    11461 acatggcaag caggtgctac gttagtgcca ggatttttag caaaagaagt tatcgtaagt
    11521 tcgatggcga ttatctattc atcaggtgat gcaggccttg ttaatgtgat tcaaaatcaa
    11581 ttcacaccgc tatctgcgta tgcatttatg atatttatac tattgtatat tccttgtgtt
    11641 tctacagtgg cagcaatccg taaagagact tattcatgga aatggacggc acttgcagtt
    11701 gcctatcctc tagtcactgc atatgtctta acattcattt tttatcaagt cggacattta
    11761 tttgtttaag gagtggttaa agtgtcagtc attattaaca ttttaatttt tttagcaatt
    11821 ttcggatatg ccttgtatac actagtgaaa tttttcaaac gttcgaaaca aggaaaatgt
    11881 ggtacatgtg acattgatcg tgattgttgt ggaacagaac aacacacagc gaatcatttt
    11941 cctgggaaat aatttaaaaa atgagcacag gcgagggcgt cctgatgctc attttttgat
    12001 acgctatatg ttaaattgtc gttatttacc ttttacgact aattcgttat cctcaacatc
    12061 tatatccaca tgtttaacgt ctgtatgatc taaataataa tctgttattt tgtcacgcac
    12121 ttgttgttca acaatacgtc ttaatgggcg tgcgcctaat tcttcatcat aaccttcttc
    12181 aattaaccaa tctttcgcat cttgtgaaac gtccatcgta ataccttttt tatctaatgt
    12241 aacttgtaca tcgtctaata acaagttgac gatatcttgt aatgcatctt tatctaaatg
    12301 taagaattca acgatgccgt tgaagcggtt aaggaattca gggcggaaga attttttcat
    12361 ttcatgcata atatcttttt cttcagtgtc attgccattg ccaaagccag catttgatgt
    12421 acaaataata attgtatttt taaagttgat gacattacct tgaccatcag tcaaattacc
    12481 atcatccatc acttgaagta ataatgttaa tatttgtgga ttagcctttt caatttcatc
    12541 aaatagaatg actgaatatg gattgcgtcg tactttttca gttaatgtat ttgaattatc
    12601 gtcataaccg acataaccag cagttgtacc aatcatttta gaaacagcag tagtatcact
    12661 atattcactc atatcaagtc gaatcagtgc ctctttatta ccaaataaat caatggctaa
    12721 ctgtttggca agctctgttt tacccacacc agtaggaccg acaaatagga agctaccaat
    12781 tggacgatta ccatcatcaa aaccagctcg attacgacga atggcacgag aaaccatttc
    12841 tacagcttta tcttgaccga tgattttact tcttaaacga ctagaaatat tttttaaacg
    12901 ttcaatgtca ttggcatcca tttgagatac aggaatacca gttaatcgtt gaatggtatc
    12961 agaaatatca tgcaccgttg ctactgcagt atgttcacca ttactgtttt ctaatttatc
    13021 ttgtagagat ttaatttctt tttgaatgtc atctgctttt ttatattctt cagcacttac
    13081 agctttacgc ttatcatgtt ccaattcaga aatacgtttt tcagtcgtga ctttatcaat
    13141 agcaggactt tgtgcagata aatgtgcagc tgtaatatct aatacatcga ttgctttgtc
    13201 aggtaatagg cgttgtggaa tatattgaat ggataagtct acacatgcct ttaatacgtc
    13261 atcaggtaat ttgacttgat ggtgtgtttc aaatttttcg cgaattcctt ttaaaatttc
    13321 aaccgtatct tttgcgcttg gttcgttaac cagtacctcg ttaaaacgac gtgttaatgc
    13381 agcgtctttc atgatattat tgcgatattc atcttgagtc gttgcaccta taattgaaat
    13441 ttcaccacga cttaatgctg gttttaaaat atcagataac cctttgctac ctgaatcact
    13501 tcctgtggca ccggaaccga tgatttggtg aatttcgtca aagaagagca cagcattttg
    13561 tgatgactta acgccttcaa ctaacttttg gatgttttct tcaaaggcgc cacgatattg
    13621 cgttccagct tcaagcgatg aaatatctat agatataatt tctttatctt tgattgctgc
    13681 aggtacgttc ccttcaacga ttgcttgcgc taaaccttcg acaatcgcag ttttaccaac
    13741 tccagcttct ccgactagga taggattgtt tttagtgcgt ctacttaaga cttctgcagt
    13801 ttcttggatt tctttatcac gaccgatgac tggatctaat aatccgtcac gagcctcttg
    13861 cgttaagtta cgaccaattt gttctaaata attgccattg ccactttgtt gaccttgttg
    13921 tcttagtgct gcttgttgaa aagcttgcgc actttgttcg gcagaatgat taccaccttg
    13981 ttgcgtaaat tgtgctaatt cttccggtga aacttcttta ccattaatat agtatttctt
    14041 gttactagtc tgatttgagt tttgcatgtc ttgcatcatt cttcgtaaaa tagaatcgaa
    14101 gtcactattg aaaaaattgt tattcataaa catcacctct aaaattttaa actattaagt
    14161 aaaagaaaat atattgaaac tatatgcaaa ctataaacaa tggcaattct atttttccac
    14221 cattgtagtt ttaaaaacgg cgcattgtag gcactttatg aactttttgt gtaatttttg
    14281 aagtaagtaa gaatttaaaa gtaaatatta atcaatggtt aagaaacgta tgtgttgata
    14341 catagttaat taagaaatgg tatattgtgt tggtagtcta tgtgaatttt gatttggaag
    14401 tgaatagttg atcttgataa agcataaagt gttaatcatt ttcataaagt taaatgaata
    14461 gtatgatgat gaaaaaggag tgtgtgttta tggtatataa gagttactat gattctcctg
    14521 taggacgatt ggaacttgtg agtgatgggg taagtctgac cgctgttttg tttgaaaatc
    14581 aacaagatga tggtactagg gaagaaaata cgtctttagc gatatttaaa gaggcgacac
    14641 agtggttaga cgcgtatttt aaaggtgaca atcctgaaat cacaatacct ttaaagccaa
    14701 caggtagtca ttttcaacag tgtgtttgga atgaattaag acagattcct tatgggacgt
    14761 taacaacgta tggtgctatt gccaaaaaag taggcaagct gcttgataag ccaaaaatgt
    14821 cagcccaagc ggttggaggt gcagtcggaa gtaatccatt atctattatc gtgccatgtc
    14881 atcgcgttgt cggtaaaacg ggtagcttaa caggattcgg tggaacgatt aataataaaa
    14941 ttaagctgtt agagttggag aatatcgata tgtcaaagct ttatgtacca aaacatagca
    15001 cgaagccgta acgtataaag gattcaggca ataggaaaac tttaataatg tgtcaaatag
    15061 gcattatgaa ttcattcaag acgaagcatt ataaataaaa aacagcagta agacgatttc
    15121 aatgagaagc tgtcttactg ctgtttttag gttatattta aatgtctttc aaatacacag
    15181 attactctgg tctgtgatat tcgcgaacgt tattttcaat atttgataag tagaaaatat
    15241 gacgatcttc atgaacagca tcttgttgtt cgtcaaagtc tacgtcatca aaacgtttga
    15301 agaatgtttc atatgcatca acagatactt cagtacggtt atttaataat gctttgtgcg
    15361 cagcctgatc taaatgatct ttgtagcctt caactaatgt cgcactataa aattcaccaa
    15421 ctgaacctga gccataactg aataaaccaa ttgtttcacc agcttgtaaa tcacgatttt
    15481 caagtaatga tattaggctt aaatataatg atccagtata aatattaccg acataacggt
    15541 tataatctac agcatcttca tatcctgaac gtaaacgctc ttgagttgtt tcatcagcgt
    15601 tatcaatgat tgactctaat gcctttttac ccatttttgt aaatggaaca tggaagcata
    15661 gtgatgcgaa gtcagctagt gacttacctt gacgttttgc gtattcattc cagctttgtt
    15721 ggaatgagcg gatataagca tctttagata atgcaccatc aactaatgga tatttatgtc
    15781 cagttggacg ccagaaatca taaacgtctt cagtgtaagc aacagcatct tcatttaatg
    15841 ccaaaatgct tggattatgt gaaataacca tcgcaactgc gccagcacct tgtgttggct
    15901 cgccgcctga attcaatcca taacgtgctg tatctgtagc aataactaat actttttcat
    15961 tcggtctagt tgctaaataa tctttagcta attgaattgc tggtgttgca gcataacaag
    16021 cttctttcat ttcaaagcaa cgtgcaaaag gttgaatacc taataagttg tgaatttgaa
    16081 cagcggctgc tttagcagca tcaactgctg attcagttgc cacaattacc ataccaattt
    16141 tctttttatc ttcgtctgtt ataatgtcct tagcagcgtt agcgcccatt gaaacgatgt
    16201 cttgatttac aggactaaca gccatttcag tttgacctat tccaattaaa aatttgtttg
    16261 ggtctacttg gcgtgcttct gctaatttag ccatgtctac atagtacttt ggaacgtaaa
    16321 agtttatttt gtcgatacct aatgtcataa ggttttacat cctttcaaat ttataaaagc
    16381 aacacttgta tcatatcaga atatgctaat cttatacaaa gaaatacact aaatttaaaa
    16441 atgatttgac gtttcttgta agcgttttaa tttaccttgc tttattttaa agtcaaaacg
    16501 acaattattc aattgaattt taagacgaat aacgttattt tatggtaaaa atagtagtga
    16561 ttgaaatgtt aaaaaattat gtgaagtaaa aggagtatgt ccatgcaaag tttagataag
    16621 aatttccgac atttatctcg tcaacaaaag ttacaacaat tggtagataa gcaatggtta
    16681 tcagaagagc aattcaacat tttattgaat catccattaa ttgatgagga agtagcaaat
    16741 agtttaattg aaaatgtcat cgcgcaaggt gcattacccg ttggattatt accgaatatc
    16801 attgtggacg ataaagcata tgttgtacct atgatggtgg aagagccttc agttgtcgct
    16861 gcagctagtt atggtgcaaa gctagtgaat cagactggtg gatttaaaac ggtatcttct
    16921 gaacgtatta tgataggtca aatcgtcttt gatggcgttg acgatactga aaaattatca
    16981 gcagacatta aagctttaga gaagcaaatt catcaaattg cggatgaggc atatccttct
    17041 attaaagcgc gtggtggtgg ttaccaacgt atagctattg atacatttcc tgagcaacag
    17101 ttactatctt taaaagtatt tgttgatacg aaagatgcta tgggcgctaa tatgcttaat
    17161 acgattttag aggctataac tgcattttta aaaaatgaat ttccgcaaag cgacatttta
    17221 atgagtattt tatccaatca tgcaacagcg tccgttgtta aagttcaagg cgaaattgat
    17281 gttaaagatt tagcaagggg cgagagaact ggagaagagg ttgccaaacg aatggaacgt
    17341 gcttctgtat tggcacaagt tgatattcat cgtgctgcaa cacataataa aggtgttatg
    17401 aatggcatac atgccgttgt tttagcaaca ggaaatgata cgcgtggtgc agaagcaagt
    17461 gcgcatgcat acgcgagtaa agatggtcaa tatcgtggta ttgcaacatg gagatacgat
    17521 caagaacgtc aacgtttaat tggtaccata gaggtgccta tgacattggc aatcgttggc
    17581 ggtggtacaa aagtattacc aatagctaaa gcatcattag agctactaaa tgtagagtca
    17641 gcgcaagaat taggtcatgt agttgctgcc gttggtttag cacaaaactt tgcagcatgt
    17701 cgcgcgctcg tttctgaagg tatccagcaa ggccatatga gcttgcaata taaatcttta
    17761 gctattgttg taggtgcaaa aggtgatgaa attgcgcaag tagctgaagc attgaagcaa
    17821 gaacctcgtg cgaatacaca agttgctgaa cgcattttac aagatttaag aagccaacaa
    17881 taatcaaatg cgaatagcga ttttaaagaa aagtttaagt cagtccatcg tagcgcttaa
    17941 ataaattaat atgaacaaaa aataaaaaac ttctatcaca cgcatacagg aatttgaagc
    18001 gttcgtgata gaagtttttt gattagtaaa tatagttaaa gttacgtgct gcctttttgc
    18061 ttagtacacg tgtattgtat tttaatgggc gataaaagtt gtattctgaa actttaatac
    18121 taccgttttt atatacttgt tcaacaactg cgacatgtcc ataatatcct gcagttgatt
    18181 gcatgacagc aaattttctt ggtgtacgac cagtcgcata ttttgattta cgtgcattgt
    18241 aataccaatt tttggcattg ccccatctat tagaaacagg tttaccgact tgtgcgcgac
    18301 gtttaaatgc ccaccatgta cattggtttt tactataata attaactgcg gcatgcgatt
    18361 gatgcgtttg ttcgacggat tgataaatga aatctgctga gaaaaggact gcaaaagcaa
    18421 ttagtaaacg aaaaagtatc tttttatatt tcatttttat ctcccaagtt aattttttct
    18481 ttattcaacg ttatttatga tagcaaaatt tttttagaaa acaatgcaag caatcaacaa
    18541 taaaagtaac tgtaatgaag ctgtcatata aagtgttaaa ttgtaataaa aatggatttt
    18601 ttcataaaat gtagaaattt atgtatctaa caattaagtg tcatagtgat acaaggatgt
    18661 caggattgat taacagcgga tattagtggg aaaatataaa atgatttaaa agaaaaggta
    18721 cgtggttcta taactgaaag aaaatagcaa tcacaacata taaatgaagc gataacatta
    18781 aaaacagcgc cctgcaatca gagcgctaac atgagatgat gtgactccat gtattcatgt
    18841 atcattgtac atttgtgaat tggttgaaaa ctgatagtgc ccgtttaatt acaggtactg
    18901 agatgctttc aggacatgga tttgttcgaa aatatgacaa tatttcacta tggctcttca
    18961 cttctctagg gaaattgcta tcttgattaa tccaatcaac caattcacct aatggtgtgt
    19021 catcaccaag aaaactttgc ataaattcgt aaaagctcaa tacctcacct cgaataacaa
    19081 acttgagcca ttaatataat accataaaag aatgtgtatt ttacataaaa ctttttataa
    19141 agcaatttta taaatctaaa tttattgaat gtttaagaag ttgtgtataa tgaaataaac
    19201 gtgttgaaat agttaggatg atgttagtgg atatcaaaca tatgaaatat tttattgaag
    19261 tcgtaaagca aggaggcatg actaatgctt ccaaatcatt atatattgca caacctacaa
    19321 ttagtaaagc aattaaagat attgaaaatg aaatgggtac gcccttattt gatagaagta
    19381 aaagacattt aatacttacc gatgcaggtc aaatttttta tgagaaaagt aaagaaattg
    19441 ttgcactata cgattattta ccatctgaaa tggaacgctt gaatggactg gaaacagggc
    19501 atataaacat gggcatgtcg gcagtcatga atatgaagat tcttataaat attcttggtg
    19561 cattccatca acaatatcca aatgttacat ataatttaat agaaaatggc ggaaaaacga
    19621 ttgagcagca aattatcaat gatgaagtag atataggcgt gaccactttg ccagtggatc
    19681 atcatatttt cgattatact accctagata aggaagattt gcgacttatc gtgagcagag
    19741 agcatcgact cgcaaaatat gaaactgtta aactcgaaga tttagcaggt gaagacttca
    19801 ttttatttaa taaagacttt tacttgaatg ataaaattat tgaaaatgct aaaaacgttg
    19861 gctttgttcc gaatactgta gcgcaaattt cacaatggca tgttatagaa gatttagtta
    19921 cgaatgaatt aggtattagt attttaccaa catcaatttc agagcaacta aatggagatg
    19981 tgaagctact acgcattgaa gatgcgcatg tacattggga attaggtgtt gtttggaaga
    20041 aggataaaca attaagtcat gccacaacga aatggataga atttttgaaa gatcgtttag
    20101 gctaacatat taataaagca ctcattattt gtgattcctc attacttgga tcattgaaat
    20161 aatgagtgtt tttttgtgaa aatgaagtga aatttagaga gcgtttccat agaaaatagt
    20221 aatacaaacc ataaaaaaag agtattttta tattgtgtac gccatcttta taatagttat
    20281 tgtaacaatt tagacatatt tagaaaggga tggcgccatg cacaaagtcc aattaataat
    20341 caaattatta ctacaactag gaatcatcat tgtgattact tatattggca cagaaattca
    20401 aaagattttt catcttccct tagccggcag tattgttggt ctatttttat tttatttact
    20461 attacaattt aagattgtac cgctaacttg ggtagaagac ggtgcaaact ttttattaaa
    20521 gacgatggtc tttttcttca taccgtcagt tgtaggtatt atggatgttg cttccgaaat
    20581 tacgctaaat tatatactct ttttcgcagt cattatcata ggaacatgta tcgttgcatt
    20641 atcttcaggt tatattgctg aaaaaatgtc tgttaaacat aagcaacgta aaggtataga
    20701 tgcttatgaa tgattacgtg caagccttgt taatgatttt attgactgtc gttttatatt
    20761 atttcgctaa aagattacaa caaaaatatc caaatccatt tttgaatcca gcattaattg
    20821 catctttagg aattattttt gtcttactta tctttggaat tagttataac gggtatatga
    20881 aaggtggcag ttggatcaac catattttaa acgcaacggt cgtatgttta gcgtacccac
    20941 tttataaaaa tagagagaaa attaaagata atgtctctat catttttgca agtgtattaa
    21001 ctggcgtcat gctaaatttc atgttagtgt tcttaacact taaagcattt ggctattcta
    21061 aagacgtcat tgtaacatta ttgccccgat ctataacagc cgctgttggt atagaagtgt
    21121 cacatgaact tggtggcaca gatacgatga ccgtactttt tattatcaca acgggtttaa
    21181 tcggtagtat tttaggttcg atgttattac gatttggaag atttgaatct tctatcgcca
    21241 aaggtttaac gtatgggaat gcgtcacatg catttggcac agctaaagca ctggagatgg
    21301 atattgaatc tggtgcattt agttcaattg ggatgatttt aactgcagtt attagttcgg
    21361 tgttaatacc tgttctaatc atactgttct attaattaga aaaagacact aagaaaggga
    21421 ggctattagt aataatggca aaaataaaag caaatgaagc attagttaaa gcattacaag
    21481 catgggatat agatcactta tatggtattc caggtgattc aatcgacgca gtggtcgata
    21541 gtttacgtac agtgagagat caatttaaat tttatcatgt acgtcatgaa gaagtagcaa
    21601 gtttagcagc tgctggttat acaaaattaa ctggtaaaat cggtgtggca ttaagtatcg
    21661 gtggccctgg tttaattcat ttattaaatg gtatgtacga tgccaaaatg gataatgtac
    21721 cgcaattaat attatctgga caaacgaata gtacagcact tggaacgaaa gcattccaag
    21781 aaacaaattt acaaaaatta tgtgaagatg tagccgttta taatcaccaa attgaaaaag
    21841 gtgacaatgt gtttgaaatc gttaacgaag caattcgtac ggcatatgaa caaaaaggtg
    21901 tagctgttgt tatttgtcct aatgacttat taactgaaaa aattaaagat acaacgaata
    21961 aaccagtaga tacatcaaga ccaacagtag tatcaccaaa atataaagac atcaaaaaag
    22021 cggttaaatt aattaataaa agtaaaaagc ctgtcatgtt aatcggtgtt ggtgcgaaac
    22081 atgcgaaaga tgagctacgt gaatttattg aaatggctaa aattcctgtc attcattcat
    22141 taccagctaa aacaatcttg ccggatgatc atccatatag tatcggtaac ttaggtaaaa
    22201 tcggtactaa aacatcttat caaacaatgc aggaagcgga tttattaatt atggttggta
    22261 caaactatcc atatgtggat tacttaccta agaaaaatat taaagccatt caaattgaca
    22321 caaatcctaa aaatatcgga catcgtttca atattaatgt aggaattgtt ggagatagta
    22381 aaattgcgtt gcatcagtta actgaaaata ttaaacatgt tgctgaaaga ccattcttaa
    22441 acaaaacgtt agaacgtaaa gcggtttggg ataaatggat ggaacaagat aaaaataata
    22501 atagtaaacc attacgtcca gaacgattaa tggcatcaat caataaattt attaaagatg
    22561 atgcagtgat ttcagcagat gtaggtacag caacagtttg gtcaactcga tacttaaacc
    22621 ttggtgtaaa taacaagttc atcatttcaa gttggttagg tacaatgggt tgcggtcttc
    22681 caggtgcgat ggcatcaaaa attgcatatc caaatagaca agccatcgca attgctggtg
    22741 acggtgcatt ccaaatggtg atgcaggact ttgcaacagc tgtacaatat gatctacctt
    22801 taacagtgtt tgttctaaat aataagcaat tagcgtttat taaatatgaa caacaagctg
    22861 ctggtgaatt agaatatgcg gttgatttct ctgatatgga tcatgccaaa tttgctgaag
    22921 cagcaggtgg taaaggttat acaattaaaa gtgctagcga agtagatgct attgtagaag
    22981 aggcactagc acaagatgta ccaacaattg tagatgttta tgttgatcct aatgctgcgc
    23041 cattaccagg taaaattgta aatgaagaag cgcttggtta tggtaaatgg gcatttagat
    23101 ctattactga agataaacat ttagatttag atcaaattcc acctatttca gttgcagcaa
    23161 aacgtttctt ataactgatt gaaaggttat cacaattgaa ttgaactata aaaacggtaa
    23221 tttctatttc aacaaaatgg gaattgccgt tttgtttatt tatcacaaat gattgtactg
    23281 aattgatgat aaaattgtga aaaagttgtt gaaaacgctt ttacaaatat gtataatagc
    23341 tatgaattac atatcacttg cgtgttactg gtaatgcagg catgagcaaa caaccgtact
    23401 atgagaatag tcttgtttgt tcatgcctgc ttttttgtac atggaagcgg aaattgagat
    23461 aggggatgtt tatatgttta agaaattgtt tggacaattg caacgtatcg gtaaagcatt
    23521 aatgttacct gttgcgattt taccagcagc tggtatttta ttagcgtttg gtaacgcaat
    23581 gcacaacgaa caattagtag aaattgcacc atggttaaaa aacgatatca ttgtaatgat
    23641 ttcgtcggtc atggaagcag caggacaagt tgtatttgat aacttgccat tattatttgc
    23701 agttggtaca gcacttggat tagcaggagg agacggtgtt gcagcattag cagcgctagt
    23761 aggttactta attatgaatg caacaatggg gaaagtgttg catattacaa ttgatgacat
    23821 tttctcatat gccaaagggg caaaagaatt aagtcaagca gcgaaagaac cagcacatgc
    23881 cttagtatta ggtattccaa cattacaaac gggtgtgttt ggtggtatta tcatgggtgc
    23941 tttagccgca tggtgttaca acaaatttta taatattaca ctaccaccat ttttaggatt
    24001 ctttgcaggt aaacgatttg taccgattgt gacatcggtc gtagcaattg caacaggtgt
    24061 ggttttaagt tttgcgtggc caccaattca agatggatta aatagtttat cgaatttctt
    24121 attaaataaa aatttaacat taacaacttt tatattcggt attattgaac gctcattaat
    24181 tccatttggt ttacatcata ttttctattc accgttctgg tttgaattcg gaagttatac
    24241 aaatcacgca ggtgaattag ttcgtggtga ccaacgtatt tggatggcac aattgaaaga
    24301 tggcgtacca tttactgctg gtgcatttac tactggtaaa tatccattca tgatgtttgg
    24361 tttaccagcg gcggcatttg ctatttataa aaatgcacga ccagaacgta aaaaaatcgt
    24421 gggtggttta atgttatcag caggattaac tgcattttta actggtatca ctgagccatt
    24481 agaattttca ttcttatttg tagcaccagt actttatgga attcacgtat tattagctgg
    24541 tacatcattc ttagtaatgc atttattagg cgttaaaatt ggtatgacat tctcaggtgg
    24601 tttcatagat tatattttat atggtttatt aaactgggat cgttcacacg cattattagt
    24661 tattccagtc ggtattgtat atgctatcgt gtattacttc ttattcgact ttgcaattcg
    24721 taagtttaaa ttgaaaacac caggtcgtga agatgaagaa actgaaattc gtaactctag
    24781 tgtcgcaaaa ttaccatttg atgtcttaga tgcaatgggt ggaaaagaaa acattaaaca
    24841 tttagatgca tgtattacac gtctgcgcgt agaagtggtt gataaatcaa aagtagatgt
    24901 agcaggtatt aaagctttag gcgcatcagg tgtattagaa gttggaaaca atatgcaagc
    24961 tatctttggt ccaaaatcag atcaaattaa acatgatatg gcgaagatta tgagtggtga
    25021 aattacaaag ccaagtgaaa cgacagtgac tgaagaaatg tcagatgaac cagttcacgt
    25081 agaagcactt ggaacaacag acatctatgc accaggtgtc ggtcaaatca ttccattatc
    25141 agaagtacct gatcaagtat tcgctggtaa aatgatgggt gatggtgttg gctttatccc
    25201 tgaaaaaggt gaaattgtag caccgtttga tggtacagtg aaaacaatct tccctacgaa
    25261 acatgcgata ggattagaat ctgaaagtgg tgtcgaagta cttattcata ttggtatcga
    25321 tacagtgaaa ctgaatggtg aaggattcga aagtctgatt aacgttgatg aaaaagtaac
    25381 acaaggtcaa ccattaatga aagtgaattt agcatacttg aaagcgcacg caccaagcat
    25441 cgttacacca atgattatta caaatcttga aaataaagaa cttgtcattg aagatgtcca
    25501 agatgctgat ccaggtaagc taattatgac agtcaaataa tgattgaaaa tgaaatagca
    25561 tatcaaatga atgaactttt agtcattcat agtgcgtatg cgaagtagcg agttgaaaga
    25621 gaatacgtta caaaaggcag tagcttaaaa tgaagctact gtctttttag tgcgcaatga
    25681 tgtatagcag gtgtgttgat gagaataagt taaatattag tgttaggtat agaaaacatt
    25741 gcttatgttt ttgtcacatt ttagaaaaat gcatcttcgc gactagccaa attaatagcg
    25801 tcattgaaaa gtttgatttt ccgtaataaa gttcaattgc ttgtattttt atcatgaaag
    25861 taataaaaat aaattaacat gattttaaat ctatttgtaa gataaggaga tttgtcatta
    25921 tgacaacaga aggtctatta gttgcagaga aagaaatcga agtgaatggt tacgacattg
    25981 atgcgatggg tgtcgttagt aatatcgttt atattagatg gttcgaagat ttgagaacag
    26041 cgtttattaa tcagcacatg aattactcaa caatgatcaa tcaaggcatt tcacctatac
    26101 ttatgaaaac ggaagcagaa tataaagtac ctgtcacaat acatgacaaa ccagtaggcc
    26161 gtatttactt agttaaagca agcaagatga aatgggtgtt tcagtttgaa attgtgtccg
    26221 cacatggcgt gcattgtatt ggtacacaga caggcggttt ttacagattg agcgataaga
    26281 agattacctc tgtgccacaa gtatttcaag acattttagc aacaaaataa tgacttcatt
    26341 ttaaaatata aaaagtaaga aggtgttcga aatggttaag caattaaata gtgtcgaagc
    26401 attccgtgaa tttattcatc aatatccgtt agcagttgta catgtcatgc gcgatcagtg
    26461 tagcgtgtgt catgccgttt taccacaaat tgaagacttg atgcaatcat accccaatgt
    26521 gccattagct gtgattaatc aaagtcaggt ggaagctatt gctggagaat taaatatttt
    26581 cactgtacct gtggatttaa tttttatgaa tggaaaagaa atgcatcgtc aagggcgttt
    26641 tatcgatatg caacgttttg aacatcatct taagcaaatg aatgatagtg tgaataacga
    26701 tgtcgatgag catgaatatc gcaaatgatt agtattgcta agattatgta gacattataa
    26761 cttatttccc agtaaatatt ggtagtaata agaatcagca tggtacatta atactatagt
    26821 agaaatcatc aaagaggagt gacgacaaat gcgtaaaaaa tggtctacac ttgcgtttgg
    26881 atttttagta gcggcatacg cacatattag aattaaagaa aaacgcagtg tgaaaagtta
    26941 tatgttagaa caaggtatac gattatctag agctaagcgt cgttttatgt ataaagaaga
    27001 agcgatgaaa gcattagaaa aaatggcgcc acagacagca ggtgaatatg agggcaccca
    27061 ttatcagttt aagatgccag taaaagtgga taagcactac ggttcaaccg tttataccgt
    27121 taacgataaa caagataagc atcaacgcgt tgtattatat gcacatggag gcgcatggtt
    27181 ccaagaccca ctcaaaattc atttcgaatt tattgatgaa cttgcagaaa cactcaatgc
    27241 taaagtcatc atgccagtat atccgaagat tccgcatcaa tattatcaag cgacgtatgt
    27301 gctttttgaa aagttgtacc atgatttatt gaatcaagta gcagattcta aacaaatcgt
    27361 tgtaatgggt gactctgcgg gcggtcaaat tgctctatca tttgctcaat tgttaaaaga
    27421 aaaacatatt gtgcaaccag gacatattgt attaatttca ccagttttag atgcaacgat
    27481 gcagcatcct gaaattcctg actacttaaa gaaagaccca atggtaggtg tgaatggcag
    27541 tgtgttctta gctgaacaat gggcagggga cacaccttta gataactaca aagtatcgcc
    27601 aattaatggt gacttagatg gtttaggtcg tattacatta actgttggca caaaagaagt
    27661 actgtaccca gatgctttga acttatcgca attgttgagt gcaaaaggta tcgaacatga
    27721 ctttatacct ggatattacc aattccatat ttatccagta tttccgatac cagagcgacg
    27781 acgcttttta taccaagtga aaaatattat taactaaaat aaaaaactct gatatcacct
    27841 ataaggtaac tacgttgtta aaaatagtta cttgctaaag tgattatcag agttttctta
    27901 ttcatctaat gtttcaggtt gtcttttaag ggatagtaat aaaattaaaa tcataataag
    27961 aatgatgcct acgatttgaa atggtttgaa tgccacatgt aaccatagac tgcttgcgat
    28021 aacggctacg acaggttcaa cagttccgaa taatgttgtt tctttcgctg atatgtattg
    28081 taaactgtcg ataaagaaat aaaatgcgag tgcggtacca ccgataatac caaagattaa
    28141 aaataatata attgatatgt cccatttagt gatatcaatg tgccaaattg gatggcgtaa
    28201 attcatcgca acaccagaaa taagcattgc ccacccgaca attagaatcg aaccgaagcg
    28261 gttaagtagg tctgaaggat aaattgtgta aaaagcgagt gctacaccag ctaaaatacc
    28321 ccagaataaa cttgcaggat tgacgactaa attagaaaat gaaccattcg ttaataataa
    28381 aaatgttcct aatagtgtca tgataatagc aagcacatca aataatgtta gttttgcaac
    28441 gcctcttatg acaaaccaaa taataatata gactggcgca atgtattgta gtaatgttgc
    28501 aatcgcagca ttacctgtat taatagatgc catataagca tattgtacaa ctaacatgcc
    28561 cagtatactg aagataagta atttgcctaa catacgtcga tcttggaata cgctaaatat
    28621 agaacgtttg ggttgcatca ttttgtacat aataagtaag aaaacaccac tgactacaag
    28681 tcgagcagtg acgtaccaat cgacattaat attcttatat ttgaacaaga aatcagaaac
    28741 agtaccacct aatccccata aacaagcacc gacaatggca agtataattc cttttaaacg
    28801 caaagctgaa tgattattca ccaagtatct caccccttta aaacttaaca ccaacaacat
    28861 aaaatatgtc agtgtatgat aatgatttaa ttcattatat cgacttttaa gaaagagtga
    28921 aatgagaaaa taaaaaaaat catctaagat gtagtggtct ggatgataga taaatgaatc
    28981 gatatgggaa atgaatgttg aaacacagag gataaactat ttaaatgaga tttcaggaag
    29041 actcaaatgt ttttgcgtaa gagatccatg atgagaatgc tgactatcta aaacgaaatg
    29101 tgatacatca tgtccagtca tactcgtata taaaataatg actaaataag aagtgaacgt
    29161 tataaagata agtgctaaat agatagaaat tgcaacttta acggaatgac tcaattgcac
    29221 ctttatcgcc tccaataagt tatactaaat attttcatct tcagtataac caaccattgt
    29281 taagttgatg taaatttagc tttaaaaaaa gtaaagattc aacctataga ctatcatcat
    29341 tcctatcaga aaactaaaaa taatatccta ctttaattat cttattgcaa atatatgtgt
    29401 aataagttat ttttgaatat agtgtgcggt tttataaaca cagcacaaaa ataagaaata
    29461 aatagctcga aagataaaat gtataaatat taaaagtttt tagaaatatt acttactttc
    29521 agatgtaaaa aagttatcaa aaaactgtca cacaactgtt tgaaatatag agtaaaaagt
    29581 taattagtaa atattaatga caacataagc agtttttgaa acaatccatt ttaaaaatct
    29641 ttcacaaata tgaaatattt cacttaaata tttgatttga taacagataa aagttgaaaa
    29701 cgttttctta agggtgtacg atgtttatgg tctgaaaata aaataaacat agagagaggt
    29761 ttttgggaaa tggatattct gttaggtgta ggcacattag tcttggtttt agtgatcatg
    29821 acgctatttt taaagtatgc accgtacggt aaacaaggac tacaagcatt atccggtgca
    29881 gcttgtgcga catttctacc acaagcattc ttaagttacg ctattggtgg cgtatttgat
    29941 attaaatttc tacaagatat tggggattta gcaggaagtt taagcggtat cgcagtaggt
    30001 attctaacgt gcttaaactt aggcgttgca ccagtatttg cggtcattgt tggtttagta
    30061 ttacatgact ttaaattatt accagcattc atcgcagctt atggcgtagc ttttttaatt
    30121 aaatggattg aaaagaaagt tccagaaggc ttggacttaa ttgtggtgat cttatttgca
    30181 ccagcaattg ctttcggact tgcatcaatc attacacctg gtgtgctagc aacattaaaa
    30241 caaataggta gtgctgtcac tgcggtaggt gataacaatc catatgcatt agcggtcatt
    30301 ttaggactag ttattcctgt tacaggtatg acaccgctaa gttcaatggt attaacaagt
    30361 ttattaggac tgacaggtgt tcctatggct attggagcac taacatgtac aggtagctca
    30421 tttgttaacc ttattttatt ccgaaaatta aacattggtg gaccgtcaaa agcatttgca
    30481 gtttgtattg agccattgac gcaaattgat ttaatagcgc aatatccagt acaactatat
    30541 ggtacaaatg cactgattgg ggtcgtcaac gcatgtatcg tgacatttag cggactagta
    30601 ataggtgtca aaggtatggc aacaccaatt gcaggtgcca tcgtattatt tggctttaat
    30661 aatgcagtga catcaattgt aacaatcgca acagtgatta ttgtcagcat tgtcttagcc
    30721 tatattattg gaacactaat taataagttt aatttaatga atatcaactt taaaatgccg
    30781 agcaaaaaga atcatattaa ggagagtgtt taattatggc aaagagctat gattatcaaa
    30841 gtgctttcga tattatcgga ccagttatga tgggaccttc aagttctcat acggctggtg
    30901 cagtaaagat tgggaattca gcacgcgcaa tattgggaga tattcctaaa aatattgaaa
    30961 tacattatta tgaatcattt gctcaaacac accaaggaca tggtacagat gtcgctattg
    31021 tgggtggagc aatgggatat tcaacatttg ataaccgaat taaatcttca ttagaaattg
    31081 cagctgatga tggtattaaa atagatatcg tcgaagaaga aggcgatagt attggtcaac
    31141 atccaaactg tgcttatatt aaatcatttc gtaatgatgg tcgctacatt gaaattatcg
    31201 gtatttcaat cggtggtggt acaattaaaa ttaaaggtat tcatataaat ggactagaag
    31261 tagatttaaa tcatggatta ccaattttag ttgttgacgg aaatatgact aaagcacaag
    31321 taaatcattt cattaatgat atcaacgata tgaaattaga ctgtaaagat gaatttatta
    31381 tgatagatga agataaatgc ttggttgtaa taccattaaa taaagcaatc tcagaatcga
    31441 cattaaatac aattaaagag aaatacagtg acgtaaacgt ttcctatata aattagaggg
    31501 ggaataaaca tgtttgattc aattagagag actatagatt atgccgtaga aaataatatg
    31561 tcatttgcgg atatcatggt taaagaagaa atggaattaa gcggtaaatc acgtgatgaa
    31621 gtgcgagcgc aaatgaaaca aaatttagat gtcatgcgag acgcagtaat aaaagggacg
    31681 acaggtgatg gcgttgaaag tgtaacgggc tacactggtc atgacgctgc taaactacgt
    31741 gattataatg aaacacatca tgctttgtct ggatatgaaa tgattgatgc agtcaaaggt
    31801 gccattgcaa caaatgaagt caatgctgcg atgggtatta tttgtgcaac gccaacagct
    31861 ggttcctcgg gtaccattcc cggtgcactt tttaaattag aaaaaacaca tgatttaaca
    31921 gaagagcaaa tgattgactt cttattcact tcagcattgt ttgggcgtgt cgtagcaaac
    31981 aatgcaagtg tagctggtgc aacaggtggc tgccaagctg aagttggttc agcatctgca
    32041 atggcggcag ctgcagcagt agctatattc ggaggatcac cagaagcatc cgggcacgct
    32101 atggcattag cgataagtaa tttattaggt ttagtttgtg acccagtagc cggacttgtt
    32161 gaaattcctt gtgttatgag aaatgcaatt ggttcgggta acgctttaat ttcagcagat
    32221 ttagcattag caggtattga aagtagaatc cctgttgatg aagttattga agcaatggat
    32281 aaggttggtc gtaaccttcc tgcatcatta cgtgaaactg gattgggtgg actagcaggc
    32341 acaccaactg gcgaagcaat taaacgtaaa atctttggca cagctgaaga tatggttaaa
    32401 aataattaat gagataacaa taaaatattg ataaaaaaga agcatctgtc tggatcatta
    32461 gtaccagata gatgcttctt aattatcttt cataaagttg aaaaatttat gggaaatgca
    32521 tgctaatcaa aatagtgatt gcaaaagcgc ccccgtatat ctaaaataac ttaaaaggag
    32581 tgatgatatg ctaagactat caatcatctt tatagcattc atcattaaca caacgattac
    32641 atatggatat acgactgagg gaacgtgggt caatctatta tttaaatctt tatcgctcag
    32701 catgattatc gttttcatgt tctactatat tcgttttgtt attgagaaaa agcgttaaat
    32761 caaagctatg gggatagtat ttgaaaagaa taaataaaag ggacgatgta aatcaatgta
    32821 atatgctcta caaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag
    32881 cttttttcga ttctagtcag gggccccagc acagagaatt tcgaaatgaa attctacagg
    32941 caatgcgagt tggggtggaa cgacgataaa gaaatacttt ttctatagaa aaagtatttc
    33001 ttatgcatga gttttactca tgtattccta tttttaagta cacattagct gtggctaatg
    33061 tgtaagaacc actacataat aaatcattag tggctcttta tcatttctgt cccacttccc
    33121 tgagaagttt aaaattttat atgttggctt gttatgttaa gtgaattaac atggttgtct
    33181 tgtttatatt atgtgattca aatattacta gtcttgtaaa tctaattcgt aaaatgctaa
    33241 atctaaccat tgattaaatt taaaacctac attggtcagt gtgccggcat gcttaaaagc
    33301 aaacttttga tgcaactgaa tactcgcttt gttggaagca tcaatgcctg caactagtgc
    33361 acgataacct ttagcttttg cttccacaat taaatggtgt agcaattgac tagcaatacc
    33421 ttttcctcta gctgaagcat cgacataaat agaatgttcg attgtatata ggtatgctgg
    33481 ccaaggtcta aacgaaccga acgtcgcaaa ccctaagaca cttccatttt cctcaaatac
    33541 aaagataggc tcatgcttac gttgtttcgt ttcaaaccat gcgacacgtt cgtctatggt
    33601 ttgtggttca taagtataaa cagctgtagt attgataatg gcatcattgt atatcgctaa
    33661 tatagcgttt aaatcctctt ttttagcgca tctaatcata acaattcccc cttagtaatt
    33721 attaaaagcg tttcgttatt tgaatgcaaa tatgtgtaat gaaatctaac gtgacaatat
    33781 atgtgcaaat ttaatattat aaaatgaatt gctatgagtc attttataat taatggtata
    33841 ctatatgaaa tgttaatagg cattgtgaaa tgtataaaag gagccttaac gtatgaaaaa
    33901 atggacaaat cgattaatga caatcgctgg tgtagtactt atcctagtgg cagcatattt
    33961 gtttgctaaa ccacatatcg ataattatct tcacgataaa gataaagatg aaaagattga
    34021 acaatatgat aaaaatgtaa aagaacaggc gagtaaagac aataagcagc aagctaaacc
    34081 tcagattccg aaagataaat caaaagtggc aggctatatt gaaattccag atgctgatat
    34141 taaagaacca gtatatccag gaccagcaac acctgaacaa ttaaatagag gtgtaagctt
    34201 tgcagaagaa aatgaatcac tagatgatca aaatatttca attgcaggac acactttcat
    34261 tgaccgtccg aactatcaat ttacaaatct taaagcagcc aaaaaaggta gtatggttta
    34321 ctttaaagtt ggtaatgaaa cacgtaagta taaaatgaca agtataagag atgttaagcc
    34381 tacagatgta gaagttctag atgaacaaaa aggtaaagat aaacaattaa cattaattac
    34441 ttgtgatgat tacaatgaaa agacaggcgt ttgggaaaaa cgtaaaatct ttgtagctac
    34501 agaagtcaaa taatccattg tgataatgga tgaatattaa gtagaaaaca gtcttgattg
    34561 agagactgtt tttttgtttg gtgtgaggta aaaagtaatt taatatccag tgtaatgtgt
    34621 tttgcatata tgaaagcaat ttcaaaatgt gaatataggt tcattgtggt atgacaaact
    34681 tcattgcttg tcatgagatg gatataatga agacgactaa aagagcaatg aggaggataa
    34741 tcatggttaa ggctattgcg gtagatatgg acgggacgtt tttagattca aaaaagacat
    34801 acgataaact tagatttgaa gcgaatttta ctgaacttag aaatagagat attacattta
    34861 ttgctgcgag tggtaatcaa tatgcgaagt tgaagtctat tttcggggat agagatatgt
    34921 attttatttc tgaaaatggt gcagttattt ataaaggcaa tgagttatat aattataaaa
    34981 gctttaatcg tcaggtgttt caacaggttg tcgattactt aaatatgaac caaagtattg
    35041 atcaactcgt catctgtggt ttgaaaagcg cgtatatttt aaaacatact tctgaagcat
    35101 ttaaagaaga tacgagattt tattatcatc agttaaaaga aattgacagt ctacaacaat
    35161 tacctgagga tgattatgtc aaaatagcat ttaatattaa tcgtgagacg catccgaatg
    35221 ttgacgaaga agtagcaacg caattcagca atgatattaa acttgtctca agtgggcacg
    35281 atagcattga tattattatg ccaaacatga ctaaggggca ggcattaaaa cgattgttag
    35341 ataaatgggg aatgtcttct tcagagctca tggccttcgg agatgcgaat aatgataaag
    35401 atatgctagc attcgctaag catagttatg tgatggaaaa cagtcatgat gaagaattgt
    35461 tcaatattgc ctctgctgtc gctccaagta atgataagca gggtgtttta acgattatcg
    35521 aacaagaagt gttgaaaaag taggaagtag attatcaatg aagcagctat catgatacta
    35581 aatacatgtt aaatgaaaac tttgaatacg aatcatcaaa tattaatata tctcaaacag
    35641 gttaggcaat aatgccatag taaaataagc gcactaaaat catttgaata gatgataaaa
    35701 taaagcggat tttaatcaaa tcgattttga tacagatata caaaataaaa caattcatga
    35761 ttttgaatac ttttcttcca cgcaaatgta tacctattgg cattagcttc tacttttgta
    35821 catcaataaa aatcgaatta tcatcaataa ggttttgctt taaacattga ctatgaaact
    35881 ggataaataa agattcaatt aatgcatcag tattaggatt tactctaaaa cgattaatag
    35941 ttttataaga aggtgtttga ttttgagcta accacatcat tcgaatactg tcatgaagta
    36001 gtttctctat tctacgacca gaaaatacag attgagtgta tgcatataag ataattttta
    36061 cattattttt ggatgataag atgttgcgcc acgatggtgt ctgaattcat cgaattcact
    36121 attaggtatc gtttcaacaa tttcattaac atatcgcgaa atatcaattt gaggaattct
    36181 aacagaagtt tctattggta gtgtaagttg gatcaagtta taatttttat acataaggca
    36241 cttcattaat ttagtttagt ggtatttatt aaattatacg aagggaccca acacagaaaa
    36301 ttcaaattat tgaattatac atttatgtgc aagtttggca aagtgtctta ttttttaaa
//
LOCUS       DATTQF010000012        36226 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.012, whole genome shotgun sequence.
ACCESSION   DATTQF010000012 DATTQF010000000
VERSION     DATTQF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.012"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            4..450
                     /locus_tag="VB241_000495"
     CDS             4..450
                     /locus_tag="VB241_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1243826.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKDYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIVNNLRNFVSKNFDNSDKK"
     gene            668..1096
                     /locus_tag="VB241_000496"
     CDS             668..1096
                     /locus_tag="VB241_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEP1243827.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFTTPSFVTNIYE
                     TDELYYLEAELAGANKEDISIDFNNNVLTIQATRSAKYKSGKLILDERNFESLIRQFD
                     FEAVDKQNITASFENGLLTITLPKIKPSNDTTSSTSIPIS"
     gene            complement(1204..2373)
                     /locus_tag="VB241_000497"
     CDS             complement(1204..2373)
                     /locus_tag="VB241_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HEP1243828.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLGQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            2882..3796
                     /locus_tag="VB241_000498"
     CDS             2882..3796
                     /locus_tag="VB241_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HEP1243829.1"
                     /translation="MYKINIIRSDSVYKNILKTPLNDRDSIFTKEILVPFKKKFEVQQ
                     IPIHNDAKQTMSAIQFLDAFQKSPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFA
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPFLYGDDINKLQGRSIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVNDFWHTHTL"
     gene            3775..4515
                     /locus_tag="VB241_000499"
                     /pseudo
     CDS             3775..4515
                     /locus_tag="VB241_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4769..5422)
                     /gene="nreC"
                     /locus_tag="VB241_000500"
     CDS             complement(4769..5422)
                     /gene="nreC"
                     /locus_tag="VB241_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HEP1243830.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5445..6479)
                     /gene="nreB"
                     /locus_tag="VB241_000501"
     CDS             complement(5445..6479)
                     /gene="nreB"
                     /locus_tag="VB241_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HEP1243831.1"
                     /translation="MINEDSIQLEALLKKYYEHSIEKIVFADDSGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTAKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGVKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6503..6955)
                     /gene="nreA"
                     /locus_tag="VB241_000502"
     CDS             complement(6503..6955)
                     /gene="nreA"
                     /locus_tag="VB241_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HEP1243832.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMIIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRSLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6975..7652)
                     /gene="narI"
                     /locus_tag="VB241_000503"
     CDS             complement(6975..7652)
                     /gene="narI"
                     /locus_tag="VB241_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEP1243833.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAAGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNVQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKIHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7645..8220)
                     /gene="narJ"
                     /locus_tag="VB241_000504"
     CDS             complement(7645..8220)
                     /gene="narJ"
                     /locus_tag="VB241_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEP1243834.1"
                     /translation="MINFDNFKKYQESFGYIAQQLCFPEKLTFHPKTFEETISKDHPG
                     YDDLIAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8213..9772)
                     /gene="narH"
                     /locus_tag="VB241_000505"
     CDS             complement(8213..9772)
                     /gene="narH"
                     /locus_tag="VB241_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEP1243835.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGNHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IKKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGISYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTNQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFDTSRLERLGLTERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9762..13451)
                     /locus_tag="VB241_000506"
     CDS             complement(9762..13451)
                     /locus_tag="VB241_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEP1243836.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTDIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQSEWKPVIHDTISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESNGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILNISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13758..14735)
                     /gene="cobA"
                     /locus_tag="VB241_000507"
     CDS             complement(13758..14735)
                     /gene="cobA"
                     /locus_tag="VB241_000507"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEP1243837.1"
                     /translation="MSVNGYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYSKSTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGYFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLEKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKPDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDTFIKQQENVTYISTDRADTNTVLCH"
     gene            complement(14726..15040)
                     /gene="nirD"
                     /locus_tag="VB241_000508"
     CDS             complement(14726..15040)
                     /gene="nirD"
                     /locus_tag="VB241_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEP1243838.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15044..17449)
                     /locus_tag="VB241_000509"
     CDS             complement(15044..17449)
                     /locus_tag="VB241_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1243839.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHDIKLITNDPVVDVDRANQNVTTANGIEVAY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPTDLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17495..18247)
                     /locus_tag="VB241_000510"
     CDS             complement(17495..18247)
                     /locus_tag="VB241_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEP1243840.1"
                     /translation="MNGNIIVAHGMRRGRQNQALEAFISELVKDDIHHYHIAFLESEH
                     QDLETVMTTLIQSGVKQLKIVPLLLFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMTEIIMQRIDEVRVDEMQQVGVVVVAHGNINGKFTKAHEELQTCVQHLQINKPT
                     YARTLYGEISFIHDLEDIARQYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLNDSNENIKALTKS"
     gene            complement(18475..19011)
                     /locus_tag="VB241_000511"
     CDS             complement(18475..19011)
                     /locus_tag="VB241_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1243841.1"
                     /translation="MANTTIRLANENDAVALHQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEETFLRDTLKAAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19478..20302)
                     /locus_tag="VB241_000512"
     CDS             complement(19478..20302)
                     /locus_tag="VB241_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP1243842.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSLNKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20505..20687)
                     /locus_tag="VB241_000513"
     CDS             complement(20505..20687)
                     /locus_tag="VB241_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243843.1"
                     /translation="MAQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSNLKKNK"
     gene            complement(20773..21240)
                     /locus_tag="VB241_000514"
     CDS             complement(20773..21240)
                     /locus_tag="VB241_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEP1243844.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSEFNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21426..22973)
                     /gene="adcA"
                     /locus_tag="VB241_000515"
     CDS             complement(21426..22973)
                     /gene="adcA"
                     /locus_tag="VB241_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEP1243845.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHGKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGTKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(23265..23864)
                     /locus_tag="VB241_000516"
     CDS             complement(23265..23864)
                     /locus_tag="VB241_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEP1243846.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKFRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(23883..24245)
                     /locus_tag="VB241_000517"
     CDS             complement(23883..24245)
                     /locus_tag="VB241_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HEP1243847.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            24500..25750
                     /gene="fmhA"
                     /locus_tag="VB241_000518"
     CDS             24500..25750
                     /gene="fmhA"
                     /locus_tag="VB241_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HEP1243848.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESAEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPILYKLQTYLKHKRR"
     gene            complement(25847..26578)
                     /locus_tag="VB241_000519"
     CDS             complement(25847..26578)
                     /locus_tag="VB241_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1243849.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVGKGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVFVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKAEAREKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAREVSNNIVFIHEGVIGEQ
                     GAPEEIFNNPKTEELKRFLNVINEE"
     gene            complement(26575..27294)
                     /locus_tag="VB241_000520"
     CDS             complement(26575..27294)
                     /locus_tag="VB241_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP1243850.1"
                     /translation="MFLNLNSEQLHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKVLRSIARVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYRQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27275..28054)
                     /locus_tag="VB241_000521"
     CDS             complement(27275..28054)
                     /locus_tag="VB241_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP1243851.1"
                     /translation="MKRLLFVVIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKAEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFNDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSKSAFAF
                     SKKVDDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28175..29614)
                     /gene="mdeA"
                     /locus_tag="VB241_000522"
     CDS             complement(28175..29614)
                     /gene="mdeA"
                     /locus_tag="VB241_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HEP1243852.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGIVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIEMMNQIKIDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSHQ"
     gene            29788..29892
                     /locus_tag="VB241_000523"
     CDS             29788..29892
                     /locus_tag="VB241_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEP1243853.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30045..30731)
                     /locus_tag="VB241_000524"
     CDS             complement(30045..30731)
                     /locus_tag="VB241_000524"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEP1243854.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIDIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(31058..31924)
                     /locus_tag="VB241_000525"
     CDS             complement(31058..31924)
                     /locus_tag="VB241_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP1243855.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(32424..32528)
                     /locus_tag="VB241_000526"
     CDS             complement(32424..32528)
                     /locus_tag="VB241_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243856.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKKVLYGEMQ"
     gene            32623..33936
                     /locus_tag="VB241_000527"
     CDS             32623..33936
                     /locus_tag="VB241_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein sbi"
                     /protein_id="HEP1243857.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVQKHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNKYYQYKGLIDKTVLTTIGSGYGSYIKPLEVSKESGNLAKSYAQVRNYVT
                     ESINTGKVLYAFYQKPELVKTAIKAQETATTFKNALTGIFKSFWK"
     gene            34472..35401
                     /gene="hlgA"
                     /locus_tag="VB241_000528"
     CDS             34472..35401
                     /gene="hlgA"
                     /locus_tag="VB241_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HEP1243858.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHEKGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            35951..>36226
                     /gene="hlgC"
                     /locus_tag="VB241_000529"
     CDS             35951..>36226
                     /gene="hlgC"
                     /locus_tag="VB241_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HEP1243859.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGNDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYY"
BASE COUNT        11900 a         6910 c         5195 g        12221 t
ORIGIN      
        1 tggatgtatg tagaaaacag ctatcttagc aaacagttgt gttttttatt ttatgtttct
       61 tcaaaagaaa taattaaaaa atacacaaac tatcttaagg attatgattt aacctatact
      121 ggttacattg ttttaatggc gattgaaaat gatgaaaaac ttaacatcaa aaaattaggt
      181 gaacgtgtat tcttagattc tggaacactg acaccattac taaagaaatt agaaaagaaa
      241 gattacgttg ttcgaacacg tgaagagaaa gatgaaagaa acctacaaat ttctctaaca
      301 gaacaaggca aagcaataaa aagtcctctt gctgaaattt ctgttaaagt atttaatgaa
      361 ttcaacattt cagaacgaga agcatcagac attgttaata atttgcggaa ttttgtttcg
      421 aaaaatttcg acaattccga caaaaagtaa ctccctatca taagatgatg ggcagaagta
      481 tgcatcgcta tgtttattca ttatctttat tgcttgtata aatctcgatg ctcagttctt
      541 ttgccctgat tccttatcag gcacaaatct atccatttat aactttttta tcccccccct
      601 tttactttat ctttttttat tctactcata taatgacttt gtaatacaaa tttatataat
      661 ggaagtgatg atattgaact tcaatcaatt cgagaatcaa aactttttta acggtaatcc
      721 aagtgataca tttaaagatt taggtaaaca agtatttaat tactttacaa caccttcatt
      781 tgtaacgaat atatatgaaa cagacgaatt atattactta gaagctgagc tagcaggtgc
      841 aaataaagaa gacattagta tcgatttcaa taataatgtg cttactattc aagctactag
      901 aagtgctaaa tataaatccg gaaaactcat tttagatgag cgaaacttcg aatcattaat
      961 acgccaattt gattttgaag ctgttgataa gcaaaatatt actgctagtt ttgaaaatgg
     1021 gctattaacc attaccctgc ctaaaatcaa accaagcaat gacactactt catcaacatc
     1081 tattccaatt tcttagttaa atttaaaatg aatacattac taaagcctta atgtcatgct
     1141 actaacgtta cacatggtat taaggcttta tcatttaaat ttaaaaatgc ttcgtattac
     1201 atatcaactg tgtttcaatg aaccatattc tttttgatat aaatggcgca ttgtaaataa
     1261 tgcaatgcat ccgaataccg ctaagaaaat aaatgctaaa tggcttgaac ctgttaaatt
     1321 agctacgtat gtgatgacca acggtgggaa gaatcctcct aaaccaccca tcattgatac
     1381 gataccattt gctgatcctg cttcatttga gaagtatgat ggtactaatt taaaaattaa
     1441 gccgttacca atacctgcac aaatacttat tgttaaacaa cctacagtaa ataatgcgat
     1501 atggtctgaa atacctaaaa taacggcacc gataatcata ataacaaaat caatcatcag
     1561 tactttaact gcattaaatt tatcacctag tataccacct attggtctca agaatgttgc
     1621 cagcgcaatg aatacacctg aacgaatacc agcatctact ttgtcaattc caaaatgatt
     1681 tactaagtag ttaggtaaga aaataccaaa tgcaacaaaa gcaccaaaag taatgaaata
     1741 ccaataactt aagtaatata atttgtagtt tttagataat gttttcattt gggccattaa
     1801 tggcacttta attttacgtt cttgtgtgtc accaaaaatg aacataatta atgcaaataa
     1861 agcgataata attaagtaac ttctaactgt tgtttgccaa ccaataatac ccgctatcgg
     1921 tggtgctaaa aatgaagaaa ctgctgtacc gatattcccc ataccataaa taccatttgc
     1981 tagacctact ttttctttag ggaaatattt aggaactgat gtaacaccaa cagagaaaat
     2041 tgcgccacct acgccaagga aaaatcctga tgccattaac atccccggcg tttgtgcttg
     2101 gcccaagaaa aatatcggga ataacaatac gataaaactt gtaaagaata cccatttagc
     2161 accaacaata tttgttaaat aaccaaatgg cacacggagc actgatccca aaataactgg
     2221 tatcgctaaa atgattgata tttgaccttc agttacattg acatcctgtt taataaaagg
     2281 cattaaagga gcaataatac tccaagccat aaatccaacc actaaactta atgtttgtaa
     2341 tgtaagttga aagccacctt ttgttttgta catacgtttc accttcacca aaatcattta
     2401 ttttttaata gatatcaata ctctgcctat atgttagagg tcaatgttat ttaaagagcc
     2461 tgggaaataa atcaatgttc catgctctac gaagttatat tggcagtagt tgactgaacg
     2521 aaaatacgct tgtaacaagc ttttttcaat tctagtcaac cttgccgggg cgggacgacg
     2581 aaataaattt ttcgaaaata tcatttctgt cccactcccc aatatagaca aagtattgac
     2641 tacacattta ttttattatg attgtgaaaa agtgaacata aggattttcc cttatttatg
     2701 gtgtaatttc ccctatacaa tgtcccttta cgatgcattt agacactatt ttgtcatcat
     2761 tttaatcaga ttattaagga tcactcacat gcatttctta aatttttttc aattagttac
     2821 aattactgct tgaaaatcac gttacgttac cctttatgct gacaacgtaa ggaggtttat
     2881 catgtataaa atcaatatta ttagatcaga ttccgtctat aagaatatac tcaagacacc
     2941 attaaacgac cgtgattcaa ttttcactaa agaaatatta gtacctttta aaaagaaatt
     3001 tgaggtgcaa cagattccta ttcataatga tgccaagcag acaatgagtg caattcaatt
     3061 tttagatgcc tttcaaaagt ctcccaaaga tttacgcatg tctgaccaaa tgtcaataca
     3121 atacctgaat aatgatttct ggagtaattg cgaaaaatat ttaaaagttg ctatagatca
     3181 atttgcaaat tatagtatct catctcaagt ttctaactat catttcacag tattacttgg
     3241 agatagccaa aaaccactta tgtatctaaa taaaaatcgc ggtggtgatg gtggcatacc
     3301 aggttatatt atgatttatt tagtgccgag tacaagtaca attaattcta tgaaaagctt
     3361 aattgcacat gaagtaaatc ataatatgcg ctatcaatat attgattggg atggcggaag
     3421 tttgattgaa ttgattatcg cagaaggatt agctgaaaac tatgtagaat cattgtatgg
     3481 taaagcgcat attggaccgt gggtaacaaa tactaattgg agtcgtgata acgtaaaaat
     3541 taaaaatact atttataatc atttacattt aaagcatata tttgaatcga tgccttttct
     3601 ctatggtgat gatattaata aacttcaagg taggtctatc gttggcttat ctcatgctgc
     3661 cgggtatgca tgtggctatc acttggtgaa atacttttta caaaaaacaa acatacctat
     3721 tgaagttgcc acaacacttc cagcacaaaa aataataaat gaggtgaatg atttttggca
     3781 tacacataca ctttaaaaga tattattgaa attacaggtg taactaaacg aactttacat
     3841 tattacgatg aaataggatt attagttcca aataaaaatg ataaaaatta tcgcgtttat
     3901 aaacagcaag acttagaaaa attacaaaaa attttaatac tcaagtcttt tgattttgat
     3961 atcgctaaaa taaaacaata catttcatat gataatgaac aattgcgaaa gttattatca
     4021 gagcaagtaa gcaagttaga taaaaagatt tctgacttat aattaattag acgctctgta
     4081 tgtgaattta ttaaaggaca ctctctaata gataccagca ttttaaacaa gtcactacag
     4141 acacaatatg ataaagaagc atctataaag tatggtcata cgaaggcata tcaatcattt
     4201 attaaccgtc aagacaactt acaatcacag gatatcagac agaaattgac aatgatcttc
     4261 aatcaattca atcaaatgtc tttaagtcat tatccaatcc aagactgtaa tgatatcgtg
     4321 gaagaatgga aatcttttat gaacacaatc gctgattttg atgatgaaac attatgctgt
     4381 attgctaaaa catatgaaaa tgatacgcgt ttcaaagatt actttaattc atatgataat
     4441 caaaatttag catcatatat ttcagaagct gttaattatt ttttgagcaa tgagaataag
     4501 aaagacaatt tttaatcctc acttagagac taaaacactg aatacaccct aactaacatg
     4561 tcatgtccaa aatagacaac tactcattta ttctctatat caataactat attaaaagtt
     4621 attccgtttc caataaaata agtatggctt tccaaaagga gaaagtatta gcttccatac
     4681 ggttataaaa aaatatattt cgaaacatac aaaagcgcca gaagataatc atttcctctg
     4741 gcgctttgta tggtattaat ttcaaactct aaaactctaa taatttcttt ttcaatgcat
     4801 attcaacgag ctctggttta cttttcaagc caagctttgt cataatatgc gttttatgtg
     4861 cctctactgt ctttacagaa acaaataact tctctgcaat ttctttattc ccgtaacctt
     4921 tggcaataag tggcaatatt tctagttctc gttttgataa gattttaaaa ggatctgatg
     4981 tgtttgcagt gtcctgattt gaattagata caaattcatt cactaaagat gttgtaagtt
     5041 tcatatctac atatgtttca cctttatata cagttcgaat agctaacaat aattgttcat
     5101 caggtgcatt tttcaatatg taacctttcg caccattacg caacacatgg aacaagtact
     5161 cttcatcatc aaacatggtt aatattaaaa ttttagtttc tggaaaactg tcagcaattt
     5221 tactcgtagc gataagacct gactcacctg gtggcatgct taaatccatt agtaacacat
     5281 caggtttata ttccattact ttttgataag cttcgacacc atctgcagct gttgcaacaa
     5341 cttccatatc attttgataa tttaaaatca tagagaatcc cgtgcggaca acagcgtgat
     5401 catcggcaat gactattttc aattttattc ccccaatgta tgtttcaaat tggaatgttc
     5461 agtgtaacat tggtgccctc accaattttc gtttcgatgt tgacgctacc actgactaat
     5521 tcagctcgtt cattcattcc atataaaccg agtccagaac ctttaggctt agaacttgga
     5581 tcaaaaccat ttcccgcgtc tattacttca gcaaccaaat ggcgcccagt ttgacggata
     5641 cctacattta tttcatttac atcagcgtat ttcaacgcat ttaaaatagc ttcttgtact
     5701 actcggtaaa caaccgtttc aatatcacta tcaaaacggg tatttttaat attggaagta
     5761 tatatgattt taaccccata attttcttca aattgtttaa aatatgattt aaacgctgcc
     5821 tcaaggccta aatcatccaa agaggctggt cttaattcca ccgacatatt acgtatatca
     5881 tcgattaatt tagcgacaat gtattcaatg ttttctgcgt cttccaaaag ctttgctgta
     5941 tcttcttgat atttcaacaa tcgcaattga acatctacat ttaacatttc ttgaatcaca
     6001 ctatcatgta attccctaga aattcgtttt cgctcatttt cttgtgcgga aatcgtttta
     6061 cgcatcatac gttgttgatg caatttttct tgttgttcaa tttgtgatga gacattttga
     6121 agcgtaaatg catgaatccc tctatcctga tcaataagct gataagttgc agtaaacggc
     6181 atcacttttt gatctttcgt cttcataaac acttgaaaat tcgtagcttg tacttgcatg
     6241 gactctaaga aacaatcttt acatgattgt acgtcatagg cattcgtata cccttcacat
     6301 ctgtgacaaa tcgcattcgc tactgcacta taattatctt cttcggataa tatatcttta
     6361 gctgcatcat tcatcgcaat aattttacca ctatcgtcgg caaatactat tttttcgatt
     6421 gaatgttcat agtatttttt taataaagct tctaattgta tactgtcctc attaatcatg
     6481 acctacaccc taattcatct cattatttat catcattgaa aattccaaat ttgcgttgaa
     6541 tatcatcgtt atcaaatatt tttggtaaag aacgtccatc tctttgacca aataatagta
     6601 caccatacac ttgatttttg taccaaagcg gtacagctaa gactgctgtt aatgattcgc
     6661 ttaataaaat tggatagtca atcttttctt ctggtcccaa tgctaaaccg acattagcta
     6721 taatcatacg cttccctgtt ttcataacag ttccagctaa tccacggcct tttcttaaaa
     6781 taattaattt aaaccgatta tttttattac ctgaaacata gtgccatttt attggagatg
     6841 atggtttgtt agattcatag aaagcgattg ccgcaaaatc ataaccttct tctttgcgta
     6901 ttttatctaa tgtctcttga aatctacgat cttcaataat tgcttctggt gtcaaatcct
     6961 ttcacctctt atacttacac tttattctta cggtaaataa tatatctgcg atttatatat
     7021 gtcaaaggta cactccaaac atgtactaaa cgtgtgaatg gccaacaagc cataatagtg
     7081 aaacctaata atatatgaat tttaaatgca attggtacac cactcattaa tgatgcatct
     7141 ggtttcaaca taaataattg tctaaaccaa attgataatg aagttctata gttaaaatct
     7201 ggatgctgta cgtttgttac taatgttgca taacatccca taaatacaat aagtaataat
     7261 aagaaattaa caaagatatc ggacgctgaa cttaatctgc gaatactttt cgtagtaaca
     7321 cgtctcgctg ttaataaaaa catccctacc aatgtaatta taccaaagat actccctata
     7381 taaacagcac caatatgata taaatgctca gacacaccgg cagcatccat ccacgactta
     7441 ggtattaata atcctacaac atgtccaaaa aataccggaa tgatacctaa gtgaaatagc
     7501 aaactacccc acatcaatct ttttctttca attaattcac tagattttgc tgtccacgaa
     7561 aatttatcat aacgataacg tgcaatatga cctgcgacaa agacaactaa acataaatac
     7621 ggaaatataa cccataaaaa ctgattaagc atgatgtttc actccttttg gtgatgtcaa
     7681 acataatttc aatgtttttc taagtgcttg aatcacatag gcatatggat tgttatcttc
     7741 accaagtgca ttcgccatca catatgttcc atcctcaata atcataatga ttaattgaat
     7801 attctcttca gctcttggat catttcgcca ttctgccact tgtaaaaatt gcaacatcaa
     7861 cggtagataa tcagaaagtt cattgtctac catttctagt ccaaacatct cgtacaatac
     7921 ctttagtttc gctaacattt gcccacgttc tttttgtgta tcaaacttgt tatacgtcat
     7981 atataatggc gctttttttg taaaatcaaa tgtatcagta taaatcgctt tgatttctga
     8041 taatgaaaat gtcatcatta catttctaaa tgctattaaa tcgtcatacc ctggatgatc
     8101 cttcgaaatt gtttcttcaa aagtctttgg atgaaatgtt aatttttccg gaaagcatag
     8161 ctgttgcgct atatatccaa aactttcttg atacttttta aaattatcga aattaatcac
     8221 ggaaaatccc tccatagaaa ttctcattat aaatttcttg accagttttt cctgaaccta
     8281 ctgcaacacc acaaccttca cagttatcac caaagtgttc gccaccatag ttatatcccg
     8341 tactaccttg agcatgatac gtatctaaat atgtttcctt atgtgacgtt ggaataacga
     8401 atcgatcctc atattttgca agtcctaata agcgatacat atctttagtt tggcgttcag
     8461 ttaaacctaa tcgctctaaa cgtgatgtat caaatggttg ttgtgtaact tgggaacgca
     8521 tgtaacttct catcattgcc atacgctgca atgcttcttt tactggtttt gtatctcctg
     8581 ccgtgaaaat attcgctaaa tattcaatcg gcaagcgcat ttcttcaatg gctgggaaaa
     8641 tcgcatccgg attttgattt gtatttttac cttcgaaata gctcataatt gggctaagtg
     8701 gtgggcaata ccaaaccatc ggcatcgttc taaattcagg atgtaatgga aatgctagtt
     8761 tatattcaat cgctaactta taaattggtg agttttgtgc agcttcaatc caatcataac
     8821 taatgccatc tttttcagct tgtgcaatga cttcttcgtc gaatggattt aaaaatatat
     8881 ctaattgttt ttcgtataaa tctttctcgt ctactgctga agccgcttca tgaactcgat
     8941 ctgcatcata taataaaaca cctaagtatc gcatacgtcc tgtacaagtt tcagagcata
     9001 ccgtaggcat acccgcctcg attctcggga aacagaatgt acacttttca gctttgttcg
     9061 ttttccaatt gaagtaaact ttcttatatg gacaacctgt catacagtaa cgccatccac
     9121 gacatgcgtc ttggtcaact aatacaatgc catcttcatc acgtttatac atagcacctg
     9181 aagggcatga ggcaacgcaa cttggattca agcaatgttc acataaacgt ggtaaataca
     9241 tcataaaagt ttcgtcaaat tggaatttaa tatcttcttc tatttttttg atgttaggat
     9301 cttttggacc tgtaacgtga ccaccagcca agtcatcttc ccagttaggt ccccattcaa
     9361 tttcaatatt atcccctgta atttctgaat atgctctagc aactggagaa tgattacctg
     9421 atttcgcagt tgttaaatgt tcataattat agttccatgg ctcgtaataa tctttaatta
     9481 gtggcatatc tggattatag aaaattttcc ctaatgcaat ttttgaaatt ctacttccag
     9541 attttaattc aagtttccct ttacgattta gtacccaacc acctttgtaa tgttcttgat
     9601 cttcccaacg tttcggatac cctacgcccg gcttagtctc tacgttgttg aaccacatgt
     9661 actcagcacc tggacgattt gtccaagtgt ttttacatgt cacactacac gtatggcatc
     9721 ctatgcattt atctaaattt aataccatcg caacttgcgc tttaatcttc aagccaatta
     9781 acctccttca tctttctaac tgctacatat aaatcccttt ggttcccaat tggtccataa
     9841 taattaaagt gataactaat ttgtgcgtat cctccgacta gttgtgttgg tttcaaatgg
     9901 attctagtcg gcgcgttgtg tgaaccacca cgtgtatctg taatttctga cccaggcgtt
     9961 tgaatatgtt tatcttgtgc atgatacata aacattgtac ctttaggcat acgatgcgaa
    10021 ataactgctc ttgccgttac aacaccatta cggttataca cttctagcca atcattatct
    10081 tggatatcgt gtttttcagc atcttcattt gatatccaaa ccgttggacc acctctaaat
    10141 agtgtcaaca tatgcttatt atcttgatac attgagtgta tattccattt tccatgaggc
    10201 gttaaataac gcagtaccaa agcatctgta ccacctttaa ttttcttatc tctattacca
    10261 aataccattg gcggcaatgt cggtttatat actggtaagc tttcaccaaa ctgttggaaa
    10321 acttcatgat caacataata actttgacga cctgttagcg ttctaaaagg tactaaacgt
    10381 tctacattcg ttgtaaatgg tgagtaacgt cggccttgtt tattagatcc cgggaatact
    10441 gctgttggta taacttcacg tggttgtgat gttatattca agaacgaaat tttttcagca
    10501 gcacgctcac tagaaatatc tttcaacggc ataccagttt gttcttcaag atcctcatat
    10561 gatttctgtg ataactttcc attcgtagct gatgaaatat ttagtatggc atcagcgaca
    10621 ttacgtgctg tatcaatacg tggacgattc gctcttacag aatcatcatt tgtatcactc
    10681 cacgtgccta acatactttt tagttcttcg tactgctcac tgacaccgaa acttacacca
    10741 tgtgctccaa ctttcccttt ttcaagtaca ggaccaagtg tgacatattt gtcgtaaatt
    10801 ttagtgtagt cgcgttctac aattgcaaag ttaggcattg tacgtccagg caccgcttca
    10861 atttcaccct tcgaccaatc tttcactacg ccatatggtg tagatatttc ttgttttgta
    10921 tcatgactaa gtggtgttgt cacaacatct ttaaacgttc caggtaaata gtcttttgcc
    10981 atttctgaaa atgcttttgc caatgtttta taaatatccc agtctgaacg tgattcccat
    11041 aatggatcaa tagcaggatt gaaaggatgt acatatggat gcatatccgt tgatgataaa
    11101 tcatgttttt cataccaagt cgctgctggc aaaacaatgt cagaatataa cggtgttgcc
    11161 gtcattctga agtctaaaga gaccactaaa tctaacttac ctgttgtttc ttcacgccac
    11221 gtaatttctt ctggcttttc atcttcattt ggtgtagcta ataatcctga ttttgtgcca
    11281 agtaaatgct tcataaagta ttcttgacct tttgcagaac ttgaaattaa gtttgaacgc
    11341 catataaata atgattttgg atgattcttt ttcaaatcag gatcttcaat tgcaaattgt
    11401 gtttgttttg atttcacttc atcaattgca cgttgcaaaa tcgcttcatt agaatctata
    11461 ccttcatctt tagcttcttc tgcaaacaac aagctatttt tattaaattg tggatatgat
    11521 ggtaaccaac caagtctagc tgctaacaca ttataatcag ctggatgttg atgctttaac
    11581 tcctctgttt tagctaatgg agattttaaa cgatctacat ttgactcttc atatttccat
    11641 tggtctgtcg caaaatagaa ccaacttgtt ccgttttgca aacgtggtgg tccttgccag
    11701 tctttcgcaa atgcgacagt actccatcct tcaatcgggc gacatttttc ttgtcccaca
    11761 tagtgagccc aaccgccacc attcacacct tgacagccac ataacataac taagtttaag
    11821 attgaacgat aaatcgtatc tgagttaaac caatggttaa tacccgcacc catgataatc
    11881 attgaacgcc cttcagtatc gatagcgttt tgcgcaaatt ctttagctac ttgaatgaca
    11941 acactttgtt ttacgcctga aatggcttct tgccaagcag gtgtatattt tgattctgca
    12001 tcgtcgtatc catttgattc caatttatga tcaaaacgac gcacgccata ttgacttgcc
    12061 attaagtcaa aaattgtagc aatacggact ttgtcaccat ttgctaaagt gacttgtcga
    12121 gttggaattg ggcgattgaa tatcccatct ccatcactat caaagtatgg gaattgaatt
    12181 gtttctaatt catacccacc ttctgtcatt gataatgtag ggttaatttt agaaccatct
    12241 tctgtttcta gttttaagtt ccatttctta ccttcttccc aacgttgacc cattgtgcca
    12301 ttaggtacca ctaaactgtc actgattgtg tcgtggatta ccggtttcca ttcactttgc
    12361 tctgtcgttt gtcctaagtc acttgctctt aaaaatcgac ctgctttata tccattttca
    12421 tcttcatcta acatgatgat aaatggcatg tctgtatatt gtttcgcata attaataaag
    12481 cgttcattcg gttgattaac ataatgctct tgtaaaataa catgtgtcat tgcttgagca
    12541 attgcagcat ctgaaccagg attcggtgct aaccagttat cagcaaactt cacattttct
    12601 gcataatcag gtgctactga aataactttt gttcctttgt agcgaacttc tgtcataaaa
    12661 tgtgcgtctg gcgtacgcgt tagtggtaca ttagaccccc acataataat gtatgatgcg
    12721 ttataccagt cacttgattc aggtacgtct gtttgttctc cccaaatttg tggagaggcc
    12781 ggtggtaaat ctgcatacca gtcataaaaa ctaagcattt caccaccaag taaattgatg
    12841 aatcgagcac ctgctgcata gctaatcata gacatcgctg gaatcggtgt gaatccggca
    12901 attctgtcag gaccatattt tttaattgta tatagtaatt gtgctgcgat aatatccgta
    12961 acatctttcc aatttgaacg cacatgtcca cccataccac gggcttgctt atactgtttg
    13021 gctttgtctt cattttcaac aatagaagcc catgcagcca cacgattgcc attgttttct
    13081 tctaatgctt cagtccataa atcccaaagt ttcccacgaa tatatggata tttaattcga
    13141 agtgggctgt attcatacca agagaatgat gcaccacgag gacagcctct cggttcatat
    13201 tcaggcatat ccggaccaca acttggatag tcagtttgtt gattttccca ggtaatcaca
    13261 ccatttttca caaagacttt ccaagaacat gatccagtac agttaacacc atgtgttgtt
    13321 cttacttctt tatcatgact ccaacgttct ctgtacattt tttcccattc tctactttta
    13381 ctttctagga tcgaccaatt cccattaaat ttttctgttg gcttaaagaa attcaatcca
    13441 aattttccca tatttatatc ctcctacgta taaaaatacg atgtgtagat gtcgtgtttt
    13501 taaatactaa aaatgcccaa gactattgct ttaattagat tgtacatttt ttcacaaata
    13561 taaaatatta gggaattacc taattactta aggaatttcc ctatcaataa cgggatttca
    13621 ttgaaataat acacaatcat gtgtggtcat gcttattgcc aatctaaatc gttcaaattt
    13681 ggcacaacga caaataaggc ttcaacacga atatattctc tcggttgaaa ccttacttat
    13741 tcatttattt ttataaatta gtgacataac actgtattag tatctgcacg atcggttgaa
    13801 atatatgtta cattttcttg ctgcttaata aacgtatcat agtaatcata ttgcgacgaa
    13861 tgatatgtac cattcgatgt atcatttggg tttagcaaac agccataacc ttcgtcatat
    13921 aaatgttcac agagcataag agcgtcaggt ttagaaccac ttactacata aaattgctga
    13981 gtcggattag aagttgttga gacattttct gtataaccca ctacttcgcc tataatacat
    14041 atacctggtt ttgcctcaat tgcgtaatgc tccagtttcc caacaatatt acttaagcgc
    14101 cctttaacga cgaattcatt aaaacaagat gcttgaaaaa caatcgctat cggataatca
    14161 atatctgtgt actgttgtat ctgggcgata attttctcta aacgtttaac tcccatatat
    14221 atcgccaacg taccaccatt tactaaagcg ttgacatcta cttcattttc ttctgaatct
    14281 ttaaaatacc ccgtcgaaaa tgtaacactt ttagcaacag cacgcatcgt taatcctgtt
    14341 tgcatcgtag caactgcagc actcgcagat gtcacaccag gtacaatttc aaacgcaata
    14401 ttaaagttgt ttagtgtgtc aacttcttct tgaactcgtc caaatattgc tgggtcgcca
    14461 cctttaagcc tcacaacctt gttatatcga cgtgctgctt ccacgataca ttcatttatt
    14521 ttttcttgct gaatgtgttt tgcatatggc tttttaccaa catctataat ctcagttgtc
    14581 gacttcgaat attgtaaaat caacggattc actaatcgat catatagtat aacatccgct
    14641 tcgcgtatta aacgctcagc ctttttagtc aaataattcg gattacctgg gcccgcacct
    14701 atcaagtaaa ccttgccgta accatttaca gacatatata tacgttcccg tctgtaactt
    14761 ctacctcata tacatctaca caaccttcat caggttcttg tacaatccca gtatttaaat
    14821 caatcttttg atcatggagt ggacagaata catattcacc actcactgtt ccctctgaca
    14881 atggcccttg tttatgcgga caaatattgt gaatcgcgtg aattgtacca ctttcagtta
    14941 aaaacaatcc tatttctttg cctttgacaa taaccttttt accaattaag ggtgtcaatt
    15001 catctaaagt tgtcactttg attttctctt tcgtttccat gtattacacc ttctccactt
    15061 caaagatttt tcgagcttgc gcattactag tgattgcttc ccaaggttca gcttcgactg
    15121 cttttttagc atccataata cgttcaaata attcattctg tctttctgga tcaagcaaca
    15181 cttctttaac attttcaaac cctagtcttc ttaaccacgg tgctgttctt tcagcataaa
    15241 tacctgtctc gcgataatat tgcatcaaag caccacataa tttgattact tcatcttccg
    15301 tttctacagt tgttaaaaat tcagcttttt caacttctgt accaccatta ccaccgatat
    15361 agatttggaa tccattttca actgagataa taccaaaatc tttaacacct gattcaacac
    15421 aacttcttgg gcagcctgat acacccattt tgaatttatg aggtgtatcg atgtattcaa
    15481 atgttttttc taaacgaatg ccaagtcgtg tcgtgtattg cgtaccaaat cgacaaaact
    15541 ctttaccaac acagctttta actgagcgtg ttttcttacc ataagctgat gctgaacgca
    15601 tacctaggtc ttcccatata tttggtaatt cttctttttt aactccatac aaaccaacac
    15661 gttgcgaacc tgtcacttta actagtggca catgatattt cttagccact tctcctagac
    15721 gaatcagttg gtctgcatct gtaacacccc cacgcatttg aggtataaca gaaaatgtac
    15781 catcattttg aatattcgca tggtaacgtt cgttagcaaa tcttgattct ctttcatctt
    15841 catgatcatg tggataaacc atattcaaat aatagttaat cgcaggtcga cattttggac
    15901 atccaccttt gtttttaaag tttaaaacat gtcgaacttc tttagatgtt tttaaacctt
    15961 tcgctcgtat ttgcgtaaca atttgatcac gtgtcaaatc agtacaacca catatacctg
    16021 caggtttagc agcaacaaag tcatcgccca acgtgtgctg taagatttga gcaatttgcg
    16081 gtttacattt accacatgaa ttacctgctt tcgttttagc cgtaacttct tcaactgtag
    16141 taaaaccatt ttccgtaatt gcatttacta tcgtgccttt atcgacacca ttacagccac
    16201 aaattgtttc atcatcagcc atatcagcaa ttgatagcga tgcctcttct ccacctttag
    16261 taagcaatga taccagtgtg tagtcttcag tagattcacc ttttttcatc atgttataaa
    16321 agcgtgaacc atcatcgata tcaccatata gtactgcacc aactacatta ccgtctttta
    16381 aaaagatttt tttatagtta ttatcaacgc tattaaatat ttcaatacct ttaatttcag
    16441 cattttcaac aatttgacca gcactatata ggtcacaccc agaaactttt aatgacgtaa
    16501 atgttgttga tcccttgtat ccgttcgttt ctttatttgt taaatgatca gctaatactt
    16561 taccttgttc atatagtggt gcaacgagtc cataaacttt gccgttatgt tctgcacatt
    16621 caccaactgc atatacatta ctatcacttg tttgcatcac atcattgaca acaataccac
    16681 gattaacatc tagacctgat tctttggcta cttctgtgta tggtcgaata cctactgcca
    16741 taacaactaa gtctgtcgga atctcgcgtc catcagccaa tttaacacct tcaacatcat
    16801 cttctcctaa gatttcagtt gtgttggctt gcatttcaaa cttcatacct tgcttttcta
    16861 gatctgcttt aagcatattt ccagctttac ggtctagctg catttccatc aaccattcag
    16921 ctaaatgtaa caccgttact tccatacctt gatctaataa accacgtgca cactctaaac
    16981 ctagtaagcc tccaccaatt acaattgctt tctttttagt cttagcaatg ttcatcattt
    17041 gttcagtatc atcaattgtt ctccagccaa taacacttgg taatgtcgaa cctggcactg
    17101 gaatgacaaa tgctttagat cctgtcgcaa atattaattt atcgtatgct acttcaatac
    17161 catttgcagt cgttacattt tgatttgctc tatccacgtc aactactgga tcattggtaa
    17221 ttaacttaat atcgtgttct tcataccact catatggatt cataattgtt tcttcaactg
    17281 tcattttatt ttgtaaaata tttgaaagca tgatgcggtt atagtttgga taaggttctt
    17341 taccaattac cgtaatatca tataaatcat tggcacgctc taatatttct tcgattgttc
    17401 gaatgcccgc cataccgtta ccaatcatta ctagtttttg ctttgccata aaatatgccc
    17461 ctttactaca taatatttat ttcaaaaaaa ggtgttaact tttcgttagt gcttttatgt
    17521 tttcattgga gtcattaaga ctttctaatc tatcgttaat aatttgtttt aaaatcgggt
    17581 cgaagttaat tgaaggtgtg atgtgtatat ctgtattaat aaccatgtca ttcatttgct
    17641 gcttcacttt gttaacaagt cttccgtcat ataaaaataa aggtacgaca attaattttt
    17701 gatactgtct cgcaatatct tctaaatcat gtataaaact aatctcacca tatagcgttc
    17761 tcgcataagt aggtttatta atctgcaaat gttgaacgca tgtctgtaac tcttcatgtg
    17821 cttttgtaaa cttgccatta atgttgccat gcgcaacaac gacaacaccg acttgttgca
    17881 tttcatccac gcgcacctca tcaatacgtt gcatgattat ttctgtcatt aaaggatgtg
    17941 tgccaagtgg ctcgcttact tctaccttta tgtctggata ccgtcgtttc atttcatgaa
    18001 cgatattcgg tatatccttg agatagtgca tggcactaaa gagaagcaaa ggtacaatct
    18061 taagctgttt aacgccactt tgaatcaacg ttgtcattac tgtttctaaa tcctgatgct
    18121 cactttctaa aaatgcaata tggtaatgat gtatgtcatc ttttactaat tcagatataa
    18181 acgcttctaa cgcttgattc tgccgaccgc gcctcatgcc atgtgcaaca atgatattcc
    18241 cattcacatt taccaaccct ttcacacgta ttgtatacca aatcatttcg tttttgtgaa
    18301 aagaatcaca ttataatgta aaatcaggga attccctgat gcctgtagtc atgcatattc
    18361 cttataaatt ttcccttttt gttaaatcaa aaaagcgacc atatatgaat ccctactcaa
    18421 catttatttg agcaagcatc aatatatcgg tcgcttgtag tgtatattat tatcttaaaa
    18481 tggtggttgg cctaatattg tttcgtcaaa gcgctcgggt atcaatactt tgcgcatgat
    18541 cacacctaaa tcgccatcat cattttcatg ttcgctgtat atttcataac ctcttttttc
    18601 ataaatttta agtaaccacg gatgcaatct tgcagatgta cctaaagtta cagcagctgc
    18661 ctttaatgta tctcgtaaaa atgtttcttc aacataagta agtaattgac taccatagcc
    18721 tttcccttca tactcaggat ttgtcgcaaa ccaccagaca aaaggatagc ccgaaatgct
    18781 tttcacactt ccccaaggat atctaaccgt aatcgtagat ataatttcat catcaattgt
    18841 catgacaaat gtagtatttt tatctatatt ttctttcaca gcatctaaat ttgcatttac
    18901 cgaaggccag tctatgccaa gttctctcaa cggtgtaaac gcctcatgca ttaattgatg
    18961 tagtgctact gcatcatttt catttgcgag tcgaatcgtc gtatttgcca tttgaatcca
    19021 cacactttct gtttatttta aatgaatcat ctttaatttt agctatgctt atcttttata
    19081 gcgtaccaac atttagcaca tgtgtcagat aacatgcctc attgatataa gataagtatg
    19141 tgatcatcaa ttatttcatt attgaaagca aagtaaaagt ctaggacatg aattaatgat
    19201 atgcacgctt caaagctttc atttttcaat gtctactttg aagcgtgcag ttcataatgt
    19261 cctagactct acaatgttat attggcagta gctgactgcg tggccccaac atagagaatt
    19321 tcaaaaagaa attctacaga caatgcaagt tggcgggggc cccaacacag aagctgacgg
    19381 caggccagct tacattaatg tgcaagttgg ggaacgatgc cccaaaaagg atgattctaa
    19441 attcattgtt gtcacaacat cttcaattat tacttcatta ctgttgctct aaacgattgc
    19501 gtttaccatt taaaaatgca taaacaagtc ctacaaaaat accgccacct acaaagttac
    19561 caaggaatgc aaaaacgata ttttttaata catgtaacca agatacagca tcaaggttga
    19621 agaacaccat acctgcatat aaaccagcat tgaatacaac gtgctcatat cccatgtata
    19681 caaagactat aacaccacaa gcaatgaaga atgcttttgt caatccgcct ttgaattgca
    19741 tagaaataaa aataccgata ttaataaaga agttacagaa aatacctttt gtaaaaatat
    19801 tcaaccaagt cgaatcgact gtctttttct gaactaaggc agttaaggct tgtgtcattt
    19861 ctggtgtcat aacatgtgca tatttcatta agaagaataa tacaaaacca cctaaaatat
    19921 ttccaaggaa acaatataat aaaatccaca tcatcttatt aagagaaaca actttataat
    19981 accagccaac tgtaaaatac ataaaattac ttgttaataa ttcagaattc gttagtacaa
    20041 ccaagaccaa tcctaagctg aaagcaattg ctcccattaa attaatgaga ccatcaacat
    20101 gtgtacttgc gaattgtgtt ttaataccaa acataaaaac agtaacaatg gacaataaga
    20161 aacctgccat catagattta agcatgtatc gtcctggtgt ttgctgtacc atcacttgtt
    20221 tcatttgaac agatgatgat attgagtcta cgattgcttt agttgaatac gtatcttcaa
    20281 ctgttttatt attttttatc acagcacttc ccccatttac tattatcaaa taaattttac
    20341 ctattaatta tattagtatt tagatgtaat tcatagatta aaattcagaa aattcttttt
    20401 accaattcaa aactaatatt tactcataaa aatagcgaga tagatatcgc gacataaaac
    20461 aattggtatc attttaattg cgctcatctc gctttatgta agtattactt attctttttt
    20521 aaatttgaaa agtaatgtaa accatatgca aatataccgt agcctacgac tgtacgcaaa
    20581 ataaatctaa caactttatt tggtacacat ttcgaagtaa cagtaattga agataaaagc
    20641 gtagtaaaaa tatataatat taatacttta atgtaacgtt gtgccatcga atcacccttc
    20701 attcaattaa taatattacg ttattattat aaactgaatg gcatataaaa tttttacata
    20761 ttttcaacaa ctttatttca ataaagcatc aagttgatcc aatgtttgat tcatcccttg
    20821 ctcaacaccc atgtttatga cttgttgagc ggcttcttta gttggaaata ccgatgttga
    20881 tgtcactgtc gttttcgttg tattgaactc ggaaaatgac aaagtaatct tcatgcttgg
    20941 catttttgta tcttttgcac cttgaggtgt cgcaaacgag tcaatatatt caattaaata
    21001 tggacgctta accgttttat attctgctaa tgtatagctt gtcattgtgg gtgttttaat
    21061 cgcgtaaaat gcatcaccgc ctgaaacagc attaaagcga aacactttgg tactagcgtc
    21121 ttttggatga aaccattttt caaataatgc ctttgtcgta taggcatcaa acaccttttc
    21181 aatcggtgct tctatcgttc ttgaaaaaat aattttatta tcttcaactt taatcgtcat
    21241 cttcccactc cctatctttt atatttactc atcttaacat gcattgagtg acatatgtta
    21301 ccaaatcatt tgttataaac tatagtaata ttaatcattc cctctgttga aattgataac
    21361 tacgaaataa aaaacacact ctattcagct attaacagtt gagtagagtg tgcaaacctg
    21421 ttgaattaat gcgctaacat ttcttctttg atttggtctt tatttaattt tgaaggataa
    21481 tatgttggcc agttatccat ttcttttaat aacgcgtcat tatcattacc cataaagata
    21541 tggaagtgtt ctgccttttt aggtgcaatg ttgtgatcgc taaattggat gaatttcggt
    21601 aagtctttat cattaccatc gaccaattta aacataaatc tcacgccacg gttaccttta
    21661 ggatatttca atgttttctt accattgtat tcatatttac cagtgtgttt cgtaccatct
    21721 ttagtaaatg taatttcatt tccttttata tcaatgttag taatatcagt tttatatcct
    21781 ttgtcataat aagcttttaa atctttagca gatttcttcg gatcattttc agctttatgt
    21841 tccatcactt catcaagcgt accgtctttt aagtaagggt aaacagattg ccattcacca
    21901 gcataatcgc ttaattcacg gtctttaact tgctcatctt taaaataccc atcagaaatt
    21961 gctttgtcgt gtttactttc agctttgtcg tctttcactt taacaccact gtctaaagct
    22021 ttaccgatat tttcaatatt cgatttcatt aatgattgat aggtaatatt atcttttttc
    22081 tgttgttctt tatttaaaga ctccatgttg tagaatttta aaggcttcgc atctgtttct
    22141 ttacgaattg tttcagtcac tttattcgca acattatctt cgtaaagaat atatttagca
    22201 ttgctatctc taatttcttt aacaatttta gtcaattctt tttgtgatgg atcttcagca
    22261 ttcatgtttt gaataccttt ttgaacaaaa ccataacgat cagctaagta tccaattgat
    22321 tcatgtgaaa tgaatactgc attaccttgc ttatctttag taacttgctt catatcgtta
    22381 tcaattttct taagatcgtc gtttaatttt ttgtagtttt tctcatagtc atctttatgt
    22441 tttggatctt tcttcactaa ttcatcttta atttctttag cgaaagtttg gttaatttta
    22501 ggatctaacc atacgtgtgg atcatatccg ccatgatgat gatgttcttc tttcccatga
    22561 tcatgtccct catgttcatg ctcttcacca tgttcgtgtt gatcagttaa aagctttgct
    22621 ttatctaatt tatcttctaa agacagtttt ttatctttat ctttaatagt agatgcaact
    22681 ttcttagcaa ccggatctaa attatcccct gtatacataa acaagtctga cttgcttgca
    22741 cttaatatat ctttttgtgt tggttcatag ctatgtaaat ctgtccctgc tggatagatt
    22801 gatgatacct tcacgtgttt tccaccaatt tgctctgcaa atgattgcaa tggataaact
    22861 gtcgttttaa ttgttacctt gccatcttta tcttttccat catcattccc acatgcggct
    22921 aatgataaag ttacggctgg tacaagaagt aacataccta atttcttttt catagtcacc
    22981 ctcctaaata gtaatcttta cgttttaaaa ttatatactc atgctactta aaatacaagc
    23041 actgttttta taaataatat aaagtcgaat catactttag gtatttacca cttttcgttt
    23101 tatctaatac atgactattg tacaaatatg atacagaaaa ctttatagta tttacgaacg
    23161 tcgtaatata agaaaaaaga caatctctat catattgcta atagaaattg cctttcatat
    23221 tgtcctaata aattggtatc acttatttat ctcaacaatt aagactattt gattttatct
    23281 tttaataatt tttcataact ttcataatca tatggatctt caactttctc gccattaata
    23341 aatgcagttg gtgtcgtttt tatatgatta tctttagcta tttttttatc tttctctgca
    23401 gctttccaag acttgctatc ttttgtcttg taatctttta taattttatt ttccgtctct
    23461 ttatctaaat gcagttgttt aatatgttta tctaatagtt cttttgttaa ccattcttta
    23521 ttttcatctt gctgggcagc aaataattgc ttttgaaaat ctaaaaatga tttaggtgca
    23581 tacatcaata ctgcatgact cgcacgtgaa ccaacaattg agtctttacc taagaaagct
    23641 aaattgacaa attggtattc cactttgtga ttatctatat aatttttacg aaactttggc
    23701 atgacttttt catctaattc tttacaataa ggacatttat agtcgccata tatgacaact
    23761 aatggtttgc cgtttttcga agatgtcgtt gctgattctt ttttaccgca agctgttaaa
    23821 attaacatgc tcactataaa taatgttagt aattttttag tcataataac tcctgttcat
    23881 atctagttcg tcatattttc ttcatgataa tccgcatcat gttgttgata atatcgtctc
    23941 gaatcaaaat cttgcttata tgatacacgt ttatcactaa aatcatatgc ataataatgc
    24001 acttcatcat cattactcaa aggtttataa gctaaaatga taattttatc cttatcatag
    24061 acataaatat tagcatcttt gcgttcaaaa tgatcaacac gatcaccgtt gccttttttg
    24121 gcaagttgtt cttgtttttg attttgaatt ttaactgctt catctatttc tttcacatat
    24181 ttatcattac cacatcctga tataaagact agtaataata ataccgtcgc cacaaatcgc
    24241 ttcaatgctc ccactcctac tatcaacttc caactaactt tcaatataat taatactata
    24301 caccgtcaaa catcaatgcg ctacaactac taccaataca tttaatataa atatccaaag
    24361 aaataacagt tatgtatagt caaagaaaaa tatatttcat tttctatgtg tatttgcaat
    24421 cagtttttga taaaataaaa aaatagcatt atatataaat tcaaaaatta cagttcaatt
    24481 cagagaggga tgggataaaa tgaactttgt aacgttgact tcggatgagt tcaatgcgtt
    24541 tacaacaaag catttttcac attacacaca atcagctatt cattacaatc atagagttga
    24601 tttaaaaggc gatgtgcatc ttgtaggggt taaagatgac aatggtcaag tgattgcagg
    24661 atgcttattg acagaagcac gcacacttaa atttttcaaa tatttttata ctcatcgcgg
    24721 gccagtgatg gattatacaa atcaatcatt agtagcattt ttctttaaag cattaacgtc
    24781 atatttaaag aaacaaaatt gtttatatgt ccttgtagat ccatatttaa ttgaaaattt
    24841 acgcaatgca gacggtgaaa ttgttaagtc ttatgacaac cgagcatttg ttagaacaat
    24901 ggacacatta ggctataagc atcaaggctt tccagtcggt tatgattcaa tgagtcaaat
    24961 ccgttggtta tctgtattag atttaaagga taaaacagaa gaccagttat taaaagaaat
    25021 ggattatcaa acgagacgta atattaaaaa aacatatgat attggtgtca aaactaaaac
    25081 gttaacgatt gatgaaacgc aaaccttttt cgacttattc catatggccg aagaaaagca
    25141 tggtttcaaa ttccgtgagt taccatactt tgaagaaatg caaaagttat acgatgacca
    25201 cgccatgtta aagttggcgt atattgattt aaacgagtat ttaaaaacgt tacaattaaa
    25261 gcaacaacaa ttaacagctg aactctcagg tgttgaagag gcattggaag aaagtcctaa
    25321 ttcaaagaaa aataaaacga aacgcacaca gctagaacaa cagctgaata gcaataagcg
    25381 taaaatcgat aatacaatag aacaaattga acaagatggt gcagtgttaa acttagcttc
    25441 tgctttgttt atctacaatg aacatgaagt ttattactta tctagtggtt caaatcctaa
    25501 atataatgct tatatgggtg cctaccattt gcagtgggaa atgattaagt ttgctaaagc
    25561 gcatcatatt gatcgctata atttttatgg cattactggt gatttttcag agtcagcaga
    25621 agactatggc gtacaacaat tcaaaaaagg atttaacgca cacgtagaag aatatattgg
    25681 tgattttatt aaaccaatca aacctatact ttacaaatta caaacatact taaaacataa
    25741 acgccgttaa cagtttatgc tgtaacatct agaatataac aaagaaccac gcatcatgta
    25801 tatggcatca tcgccatatt aaaacatgat acgtggttct atttgtttat tcttcattaa
    25861 taacatttaa aaagcgtttt agttcttcag tttttggatt gttaaaaatc tcttcaggtg
    25921 ccccttgttc tccaattaca ccttcatgaa tgaatacaat gttattagat acttctctag
    25981 caaaacgcat ttcatgtgtc acaatgacca ttgtcatgcc ctcattcgct aaatctttaa
    26041 tcacctttaa cacatcattc acgagttcag gatcaagcgc tgatgttggt tcatcaaaca
    26101 acatcacttt aggattcata gctaatgctc tcgcaatggc aacacgttgc tgttgaccac
    26161 ctgataatgc atgaggtcgt tgatctttga catgtgtcaa accaactttc tcaagtaatt
    26221 ctagagattt ctcacgagct tcagcctttt tcaacttttt aactgtaata agaccttcca
    26281 ttacattttc taatgccgtc ttatgcggaa aaagattata gctttgaaat accataccag
    26341 attgtttacg aacttctatt tgtgattttt tatctttaga tgtatatgtt ttgccgttaa
    26401 caaatactgt accttcagtt ggaatttcta acgcattaat cattcgtaac aacgttgttt
    26461 tacctgaccc agatcggcca attaaggtta caacctcacc tttgccaaca gataaatcaa
    26521 tacctttgat gacttcaaca tcattaaatg atttatggat attgttcaat tgaatcatga
    26581 gcgataccct ctttcaatat aagattcgta gataccttgg attattgaaa taataaagca
    26641 taccacccag tacattagcg caactaacac ataaattgtt aaatattcat atgttgtaga
    26701 cgcaacttct tgagcttttc taaacatttc tgctactaaa ataaatccta gtaatgatgt
    26761 atctttgatt aaacttaaaa atgtattacc tagtgcaggt atagatactc gaattgcctg
    26821 cggtaagatg atgcgttgta tcgtttgacg atatgtcata ccaatagaat atgcagcttc
    26881 cgtttgtcct tttggtatag aaataatacc gccgcgaata atttctgaag catacgcacc
    26941 tacatttaat gacaagccaa taatggcagc gaccacaggc gccaatgtcc attgattatc
    27001 tgcatcattg gttaataatc tacctaattc tggaatacca taaaagatga taaataattg
    27061 tactatcata ggtgtcccac gaataataga tacatagaca cgtgcaatac ttcttaaaac
    27121 tttactcgtt gaaatgcgca tcaatgctgt aaataatgca atgattaacc ctaatacaaa
    27181 tgttacgagg gtaatcggaa ttgaatattt caccaaacct tcgagcatag gcccaaaagc
    27241 ttgttttgca gcatcaagtg catgtagttg ttcactattt agatttagaa acatcttgac
    27301 caaaccattt cttacctatt ttagctaatt caccgttttc ctcgattttt ttcaagccat
    27361 cattgaattt ttgaactgtt tcgtcatcca cttttttaga aaatgcaaat gcagatttac
    27421 tttgttcagc gttacctttg attgccttga tcttagcatt aggtttttgt tttttataat
    27481 ctaagtatga cagactgtca ttaaatgtac catcaacacg cttagacaat aataaatcca
    27541 ttgattggtt aaagccatca acttttgtaa tatcagcacc tttatcctta gctaatttac
    27601 cataattaga tgtgaatgtt tgtgctaact tcttaccttt aacatcatta aaatctttaa
    27661 tatctttttc attttcacga ataactaaaa cagcacttga gaatgtgtaa ggcttagaaa
    27721 atttatattt cttttctcta tcaggattaa tacccacttg gttcgcgatt acatcaaaac
    27781 gccctgcgtc taaacctgca aacatagagt cccaagaagt ttcgttaaat ttaagcttta
    27841 atccttctgc tttagccact gctttaataa catcgatatc ataaccagtt aatttgccat
    27901 ctttatcatg gaaactaaat ggtgcataag taccctcagt accaacatta attgtcttac
    27961 tatctttact agcttccttg tctttagacg aattgttccc acatgcagct aatatgaaaa
    28021 cgaaagctat caccacaaat aaaagtcttt tcatagaata tatctcctta ttcttattat
    28081 tctaatcgga ataaaaatat tttaatctga atcgccagat aagtcaatat gttgaacttt
    28141 tgctaaaaag cgttatatag ctagcaactt atatttattg atgtgaatca acttcttttt
    28201 tctttccttg taacataaat gaacaaaaga aactaatcac tgtaatcaat agcgcaaata
    28261 aaaatccagc gtgataacca tgtaacatcg tatcaatttt aatttgattc atcatctcaa
    28321 ttttgttcat gccattatag tctgacatag ttggagaaaa gtttttactt gcatgagata
    28381 aaatagtaat taatgcagct gttccgatag aaccagcaat ttgttgtact gtgtttgtca
    28441 tagatgaacc gtgtgcattc atttctggct ttaattgatt catcgtatgt gtcattactg
    28501 gaattaatcc taacgcgata ccaaccattc gaatcgcata aactgttgcc aacatgattg
    28561 ttgatgtttg ttcatccatt acaacaaaat aagccgtagt aataacaaca atcgccatac
    28621 ctactaaagc aagctttcta ggaccaaact tttcatataa agcacccgaa gtcatcgaca
    28681 taaatgccat aacaattgct cctggtaata aagttaaccc agaatctaaa gctgaacgat
    28741 gcaataaatt ttgcacaaag ataggtaaaa tcgtttccga accaatcatc gataccattg
    28801 tgacacccat aataatgata ccaactgtaa attgtttatt tttaaatact gcaaaattta
    28861 ataaaggtgt tgatagccga tattgacgta atacaaataa tgcgactaaa acaataccac
    28921 caatcataga tactaatacg ataggattat caaatccttt ttctgaaatt gaactcgttc
    28981 catacaataa cccgccgaaa cctagtacag aaaaaatgac agaaataaaa tcaataggta
    29041 ctttaattgt agttccaaca ttttttatcg ttttaaatcc aattaaaaat gtcacagctg
    29101 caattggagc gacaacatgg aaaagcgatc tccagttaaa atattcaaca agataacctg
    29161 aaagtgtagg tccaatagct ggtgctaaac caattaccaa accaaatatc cccatatatt
    29221 taccgcgttc atgcggttgg aaaacatcca aaatcgttgt catcatcaat ggcatcataa
    29281 tacctgcacc taacgcctga ataattctag caactaataa tgttgcaaag tgcggactca
    29341 aaccgccgag taatgtacct aagaaaaaga tagaaatacc cactaaaaac acttgtcttg
    29401 ttgtataacg ttgtataaca agtgcggaca aaggaataac aatcccatta gtcaataaaa
    29461 aagccgttgt cagccattga acttgtgtat atgaaatttc aaaatctttc ataatacttg
    29521 gtaaagccgt cactaatagt gtttcattta ataatccaaa aaatccaccc atcagcatag
    29581 taataatcat cgttaatttt tgtttcttcg acatttaaag cttccccctc accatctaaa
    29641 aaacgacaca catttttaga tatccggtaa ttatacccat tttcaaaaaa aatagcacga
    29701 ctttattaca atttctttaa aacgtaatca ttactattta catatgagta gttatcggtc
    29761 ataatatatt tacaaggagg gatagagatg aaattagatt tacagactgc tcgtcgtaat
    29821 ttaaatagcc ctaacattaa aacaagaaag cgtgctttaa agattattaa gcaacacaaa
    29881 agagcgaaat aattgtttta atttgactat attgtatgtc taactatatt ttcgagtatc
    29941 cctagctcgt taatataaac tctatactta actatttcct tacattactt cacatattta
    30001 agagctgcat atacactcac tccttgtgta catgcagctc ttttttataa gtagtattta
    30061 tctataactt ctaaatcatc cgttaattca taaacaagcg gtgcacctgt tttaatttca
    30121 taattaatga tatcttcatc tgacacatct tcaagatatt taatcaatgc gcgaattgaa
    30181 tttccgtgtg cagaaactaa taccgtttga ccatctagca aatattgtga aatatgatct
    30241 gtccaaaatg gtatcactcg aactaaagta tctttcagac tttcggaata aggcatcata
    30301 cgtttatcta aatgattata tcgacgatca gctaagtaag cttcacgttg ttcttcggtt
    30361 tcagcaggtg gtttcacatc ataagaacga cgccaaatat gtacttgttc ctctccaaat
    30421 tcttttctag catcatcttt atttaagcct tgcaatccac catagtgacg ctcatttaaa
    30481 cgccagcttt tatatacagg aatccattgt tgtttagatt cagttaaaat ataatgcgtt
    30541 gtatctaaag cacgtgttaa taacgatgta aaagctacat cgatgtcaat gttattttct
    30601 cttacttttt cacctgctct agtcgcttca ttaatacctt gttcagataa attaacatct
    30661 tcccatccag taaataagtt tttagcattc cactcgcttt gtccatgacg acataaaatt
    30721 aattttggca taacttataa cctctttcct ttaatcttta ggacgttatt tcagggatat
    30781 tttaagatga tttaatctta gcaattttct tttcaatatg ttttaaaatt caatgattaa
    30841 atcaattatc actagacatt aaatacataa atcctattcc ccattttcat tttttaattc
    30901 ataaatgaat caataaccac ctaataacaa atcatattat acacctttgt ccgtcatttt
    30961 tctaaggttt aaaaaatatt tttaggtgcg cctaaaaata aatgtaataa aaacgcctcc
    31021 tcagatattt atatatctat gaagacgttt aaatacatta tagatggtct ggttctgggt
    31081 gaacgtatac tgaggaaata ccttttttgt gcaaatgatg ttcgacatta tcacaaattt
    31141 gatgcgcttc tactaaggaa aggttagcat ctacaacaat tgtgacatca ataaacacac
    31201 tacttccatg gtaacgccct ttaatacttt taacttcttg tacttcatca acttctaaaa
    31261 tatcattgcg atacgcttct aattcagttt cattgaaacc atcactcaac ataaaaattg
    31321 cttctttaaa aataccaaaa ccagtataaa cgattagtaa gcctagcaat gttgctaaaa
    31381 taatatcgac aattgggaaa ccgatttgcg taaaaattaa tcctatcgcc gttccaatgc
    31441 tgactaaact atctgataaa ttatctttgg cagcagaatt taaagaacta cttttcgttc
    31501 ttttcgctag tctttgattg actgcaaata caatcaacat tacaagacca ctgattaagc
    31561 tgacgataat tgttattgcg ttaggtacaa cgtcatcttc tttgaacaaa cgaggtgcat
    31621 tttgaataac tacttggata cctacaaaca taatgacaaa tgacaccaat aatgaagaaa
    31681 tattttcaga cttcaaatgg ccgtaaggat gatttcgatc ggcaggttta attgaaattt
    31741 tcaatccaat aataacagct aaagaaacga taatatctgt catattgttt aatgcgtcag
    31801 ctcttacagc tgcagagtta aagacaaaac ccgtgacata cttaacaata gataagatta
    31861 tatatacaat caaactcaaa taagcaccgc gttgcgccaa tttaagattt tcattatgag
    31921 acatgcgttg aaccaccttg aattagtata gtaacaatat tatgaatgat ttattttaaa
    31981 tttacaacgt ttttaatttt tattaaattt ttataaaatt aaactaattt attcattcgc
    32041 aacccctaaa aatatttttt agcctttctg cgaattttat gagctagaaa ggcttatgca
    32101 ttagtagcga agctatctaa tgcgttcctt tattttctgc ggattttatg agctagaaag
    32161 gcttatgcag ttgacttttt acgtccaact gcgttcctcc gtcttcttca aatttatttg
    32221 ttagaaaggc gcccaactct ccctgtttgt taactttcgt ctcgaaagtt tctatgttgg
    32281 gaccctatgc atgagttgcg aagctatcta atgtcttgac ctaattatat agaagaaaaa
    32341 gtgcatcagt gacaaatcaa atgagatttc tactctacca aactctcttc gaaagacaat
    32401 tttctcctct atttattagc aaactattgc atttctccat atagtacttt cttacttaaa
    32461 atacgctgaa tgttctgaat taaacgcttt tttacatagt taacactagt taatctatta
    32521 gttaacatta gttaataatt agttaatttc catttgtatt ctcatgtgat aaattctaaa
    32581 agcatacaat aaatttaata tgtaaaaaga aagggaatac acatgaaaaa taaatatatc
    32641 tcgaagttgc tagttggggc agcaacaatt actttagcta caatgatttc aaatggggaa
    32701 gcaaaagcga gtgaaaacac gcaacaaact tcaactaagc accaaacaac tcaaaacaac
    32761 tacgtaacag atcaacaaaa agctttttat caagtattac atctaaaagg tatcacagaa
    32821 gaacaacgta accaatacat caaaacatta cgcgaacacc cagaacgtgc acaagaagta
    32881 ttctctgaat cacttaaaga cagcaagaac ccagaccgac gtgttgcaca acaaaacgct
    32941 ttttacaatg ttcttaaaaa tgataactta actgaacaag aaaaaaataa ttacattgca
    33001 caaattaaag aaaaccctga tagaagccaa caagtttggg tagaatcagt acaatcttct
    33061 aaagctaaag aacgtcaaaa tattgaaaat gcggataaag caattaaaga tttccaagat
    33121 aacaaagcac cacacgataa atcagcagca tatgaagcta actcaaaatt acctaaagat
    33181 ttacgtgata aaaataaccg ctttgtagaa aaagtttcaa ttgaaaaagc aatcgttcgt
    33241 catgatgagc gtgtgaaatc agcaaatgat gcaatctcaa aattaaatga aaaagattca
    33301 attgaaaaca gacgtttagc acaacgtgaa gttaataaag cacctatgga tgtacaaaag
    33361 catttacaga aacaattaga cgcattagta gctcaaaaag atgctgaaaa gaaagtggcg
    33421 ccaaaagttg aggctcctca aattcaatca ccacaaattg aaaaacctaa agcagaatca
    33481 ccaaaagttg aagtccctca aattcaatca ccaaaagttg aggttcctca atctaaatta
    33541 ttaggttact accaatcatt aaaagattca tttaactatg gttacaagta tttaacagat
    33601 acttataaaa gctataaaga aaaatatgat acagcaaagt actactataa taaatattac
    33661 caatataaag gtttgattga taaaacagtt ttaacaacta tcggtagtgg ctatggttca
    33721 tacattaaac ctcttgaagt aagcaaagaa agcgggaact tagctaaatc atatgcacaa
    33781 gtaagaaact atgtaactga gtcaatcaac actggtaaag tgttatacgc attctaccaa
    33841 aaaccagaat tagtaaaaac agctattaaa gctcaagaaa cagcaacaac tttcaaaaac
    33901 gctttaacag gcatattcaa atcattctgg aaataatcaa tcaaaaatat cttctctagt
    33961 tttacatcat tatttaaata attttcgtaa aaaaccgtga ttaaaaagaa ccgttgattc
    34021 tcaatcgaat ctgcggttct tttttcattt tccatcaatt aaatgtttct tcgctatttg
    34081 tcagctcact tttttacctg caacttgtta aataatcctt acatcgttaa cgaatagttc
    34141 atcatttagt tgaatcagct caactttatt aacttcaaat tttcacaaac tattgcacaa
    34201 tccattcctc taccacttca agcaccttaa ttaaacatta attcaataga ataagacttg
    34261 caaaacatag ttatgtagct atataaacgc ctgcgaccaa taaatctttt aaacataaca
    34321 taatgctaaa acatcattta acaatgctaa aaatgtctct tcaatacatg ttgatagtaa
    34381 ttaactttta acgaatagtt aattcgaaaa cgcttacaaa tagattatta tatatatgaa
    34441 cttaaaatta aatagaaaga aagtgatttc tatgattaaa aataaaatat taacagcaac
    34501 tttagcagtt ggtttaatcg cccctttagc caatccattt atagaaattt ctaaagcaga
    34561 aaataagata gaagatatcg gccaaggtgc agaaatcatc aaaagaacac aagacattac
    34621 tagcaaacga ttagctataa ctcaaaacat tcaatttgat tttgtaaaag ataaaaaata
    34681 taacaaagat gccctagttg ttaagatgca aggcttcatc agctctagaa caacatattc
    34741 agacttaaaa aaatatccat atattaaaag aatgatatgg ccatttcaat ataatatcag
    34801 tttgaaaacg aaagactcta atgttgattt aatcaattat cttcctaaaa ataaaattga
    34861 ttcagcagat gttagtcaga aattaggcta taatatcggc ggaaacttcc aatcagcgcc
    34921 atcaatcgga ggcagtggct cattcaacta ctctaaaaca attagttata atcaaaaaaa
    34981 ctatgttact gaagtagaaa gtcagaactc taaaggtgtt aaatggggag tgaaagcaaa
    35041 ttcattcgtt acaccgaatg gtcaagtatc tgcatatgat caatacttat ttgcacaaga
    35101 cccaactggt ccagcagcaa gagactattt cgtcccagat aatcaactac ctcctttaat
    35161 tcaaagtggc tttaatccat catttattac aacattgtca cacgaaaaag gtaaaggtga
    35221 taaaagcgag tttgaaatca cttacggcag aaacatggat gctacatatg catacgtgac
    35281 aagacatcgt ttagccgttg atagaaaaca tgatgctttt aaaaaccgaa acgttacagt
    35341 taaatatgaa gtgaactgga aaacacatga agtaaaaatt aaaagcatca cacctaagta
    35401 aacagttcaa tcattttata aaatcctggg acacttcata cttgtctcag gattttttaa
    35461 catattgaat cagcctcata acattaaatt attttatcgt acaactgatt tttataagag
    35521 caaagtatcg catcatagta gatacacaaa taataaaaat gaaacaattt aacttgaaag
    35581 cttaaataaa tattctcaag ttaataaata attaactttt agatggattc atcaaaaatc
    35641 gcaaaaaaga acaatttgta ttttacaaac attaattaaa aataaaagca agacattcgt
    35701 gcaatcggtt gccttaaaat gtttacaact gtcaacaata ccaaggtttt attaactata
    35761 tttctcacaa aattagcttt tagcattcca aacaaaaaag gttaaattga acggaattat
    35821 ggcattttta acttaattgt aaaaaaagtt gataatggtc aattgttaat gaacagttaa
    35881 ttataataac gtccaaaata tattattatt taattaagtt aaataaaatt atagaaagaa
    35941 agtgaaaatt atgcttaaaa ataaaatatt agctacaact ttatctgtaa gcttacttgc
    36001 ccctcttgcc aatccgttat tagaaaatgc taaagctgcc aatgatactg aagacatcgg
    36061 taaaggaaac gatgtagaaa ttatcaaaag gacagaagat aaaacaagta ataaatgggg
    36121 cgtgactcaa aatatccaat tcgactttgt gaaggataaa aaatataaca aagatgcttt
    36181 gatcttaaag atgcaaggat tcattagctc tcgaacaaca tattat
//
LOCUS       DATTQF010000013        36060 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.013, whole genome shotgun sequence.
ACCESSION   DATTQF010000013 DATTQF010000000
VERSION     DATTQF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 36060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.013"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(55..120)
                     /locus_tag="VB241_000530"
     CDS             complement(55..120)
                     /locus_tag="VB241_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HEP1243860.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(152..217)
                     /locus_tag="VB241_000531"
     CDS             complement(152..217)
                     /locus_tag="VB241_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HEP1243861.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            736..2220
                     /locus_tag="VB241_000532"
     CDS             736..2220
                     /locus_tag="VB241_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HEP1243862.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVSIYIATGEWRVPQHIAHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILISIVTFIYIITHNYFYDVLQNITTHATTPPTISVIISVVILIFGS
                     LLSIWVARHRYSKTFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            2233..4938
                     /locus_tag="VB241_000533"
     CDS             2233..4938
                     /locus_tag="VB241_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HEP1243863.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLNHFSIAQEDYQAYVEGHLLALPGWAGILYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVTENDNANQS
                     HSVSLNATQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRVSLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDVMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDFVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            5100..5462
                     /locus_tag="VB241_000534"
     CDS             5100..5462
                     /locus_tag="VB241_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HEP1243864.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(5691..6107)
                     /locus_tag="VB241_000535"
     CDS             complement(5691..6107)
                     /locus_tag="VB241_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243865.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVGRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            6145..6819
                     /locus_tag="VB241_000536"
     CDS             6145..6819
                     /locus_tag="VB241_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP1243866.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTMTKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            6856..7590
                     /locus_tag="VB241_000537"
     CDS             6856..7590
                     /locus_tag="VB241_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEP1243867.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFITYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVVKIPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENIIRFFNALT"
     gene            7768..7881
                     /locus_tag="VB241_000538"
     CDS             7768..7881
                     /locus_tag="VB241_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243868.1"
                     /translation="MGIVPEVVMWRDVGDMDGVVCGEMLAQVYSVEPEILM"
     gene            8047..9384
                     /locus_tag="VB241_000539"
     CDS             8047..9384
                     /locus_tag="VB241_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEP1243869.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            9601..10506
                     /locus_tag="VB241_000540"
     CDS             9601..10506
                     /locus_tag="VB241_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEP1243870.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEMAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QHIDYFVAGIGSGGTYTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERSLVDGIFTIKDQDAFRNVKSLAKHEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKEIFNYEENDYE"
     gene            10499..11641
                     /locus_tag="VB241_000541"
     CDS             10499..11641
                     /locus_tag="VB241_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEP1243871.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIASLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGVEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTLYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            11932..12957
                     /locus_tag="VB241_000542"
     CDS             11932..12957
                     /locus_tag="VB241_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP1243872.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSPSLIRRLNDKQVGDFKDYKIFVEETQ
                     MTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDGTAINQYFKEKN
                     IQFEEVH"
     gene            12961..13620
                     /locus_tag="VB241_000543"
     CDS             12961..13620
                     /locus_tag="VB241_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1243873.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVVIVQVIQTLGNVLARFIRR
                     H"
     gene            13657..14499
                     /gene="gmpC"
                     /locus_tag="VB241_000544"
     CDS             13657..14499
                     /gene="gmpC"
                     /locus_tag="VB241_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HEP1243874.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGVYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSDSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            14833..15837
                     /gene="aaa"
                     /locus_tag="VB241_000545"
     CDS             14833..15837
                     /gene="aaa"
                     /locus_tag="VB241_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HEP1243875.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNSTSNSSRPSTNSSGGSYY
                     TVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVTGNASTNSGSTTTTNRG
                     YNTPVFNHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(16024..16293)
                     /locus_tag="VB241_000546"
     CDS             complement(16024..16293)
                     /locus_tag="VB241_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243876.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            16443..16838
                     /locus_tag="VB241_000547"
     CDS             16443..16838
                     /locus_tag="VB241_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP1243877.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESPVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYDGKHDK"
     gene            16828..17313
                     /locus_tag="VB241_000548"
     CDS             16828..17313
                     /locus_tag="VB241_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1243878.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRKRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(17482..18264)
                     /locus_tag="VB241_000549"
     CDS             complement(17482..18264)
                     /locus_tag="VB241_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEP1243879.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMIQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18261..19379)
                     /locus_tag="VB241_000550"
     CDS             complement(18261..19379)
                     /locus_tag="VB241_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEP1243880.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(19494..20378)
                     /locus_tag="VB241_000551"
     CDS             complement(19494..20378)
                     /locus_tag="VB241_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1243881.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYNESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLGPFAESVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSCIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            20559..25058
                     /gene="gltB"
                     /locus_tag="VB241_000552"
     CDS             20559..25058
                     /gene="gltB"
                     /locus_tag="VB241_000552"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEP1243882.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKH
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKFYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGNLEDALEVLGREAVDAVKQGAQILVLDDSGLVDGNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKERQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            25076..26539
                     /locus_tag="VB241_000553"
     CDS             25076..26539
                     /locus_tag="VB241_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEP1243883.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDFVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FVNGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            26812..26904
                     /locus_tag="VB241_000554"
     tRNA            26812..26904
                     /locus_tag="VB241_000554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26848..26850,aa:Ser,seq:tga)
     gene            27357..28784
                     /gene="treP"
                     /locus_tag="VB241_000555"
     CDS             27357..28784
                     /gene="treP"
                     /locus_tag="VB241_000555"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEP1243884.1"
                     /translation="MAVKREDVKAIVTAIGGKDNLEAATHCVTRLRLVLKDESKVDKE
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKKAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKELIVMYPQIADISNMIN
                     VIANTAFIFLPALIGWSSMRVFGGSPILGLVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLVVTGIA
                     IVVPCILTIVMSNFSKQKAKEIVED"
     gene            28848..30488
                     /gene="treC"
                     /locus_tag="VB241_000556"
     CDS             28848..30488
                     /gene="treC"
                     /locus_tag="VB241_000556"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP1243885.1"
                     /translation="MLKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFERLINVAHEKGIKVMLDIVI
                     NHTSTEHEWFKAARKSKDNPYRDYYFFKASEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLACEGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            30513..31241
                     /gene="treR"
                     /locus_tag="VB241_000557"
     CDS             30513..31241
                     /gene="treR"
                     /locus_tag="VB241_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP1243886.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            31303..31572
                     /gene="ffs"
                     /locus_tag="VB241_000558"
     ncRNA           31303..31572
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VB241_000558"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            31642..31743
                     /locus_tag="VB241_000559"
     CDS             31642..31743
                     /locus_tag="VB241_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243887.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYMKFDSSGMDAQFE"
     gene            31882..32406
                     /locus_tag="VB241_000560"
     CDS             31882..32406
                     /locus_tag="VB241_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1243888.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEMNSDDKTYYGLAIASLSVNPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            32475..34172
                     /gene="dnaX"
                     /locus_tag="VB241_000561"
     CDS             32475..34172
                     /gene="dnaX"
                     /locus_tag="VB241_000561"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP1243889.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            34262..34579
                     /locus_tag="VB241_000562"
     CDS             34262..34579
                     /locus_tag="VB241_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP1243890.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            34586..35182
                     /gene="recR"
                     /locus_tag="VB241_000563"
     CDS             34586..35182
                     /gene="recR"
                     /locus_tag="VB241_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP1243891.1"
                     /translation="MHYPEPISKLINSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            35524..35599
                     /locus_tag="VB241_000564"
                     /pseudo
     CDS             35524..35599
                     /locus_tag="VB241_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11891 a         5197 c         7529 g        11443 t
ORIGIN      
        1 catgtgaatg gcccccttca aataagatgt tcatatctat gatattttta taacttactt
       61 accagtgaat ttctcaatta atcctttaat gaatttaatg attcctgcaa tgatacccat
      121 gtgaaagacc tcctttgttt gttatgaaaa cttatttacc agtgaattgt tcgattaagc
      181 ttttgataac tttaatgatg ccagcgatga tacccattaa gattacctcc tttgcttatg
      241 agttaacttc attgtacata gttatcttgt gcgtaattga tttttttcct aacttgaatt
      301 gttatgatca taactgcgaa ttttttattt taaaaggacg ggaaatactg cctaactgta
      361 caggtgcatt tacaaaatct ttacaatcat tagtttagtg gggttaaggt catgaatttg
      421 acatgaagca gaaatttaat tatattttat gttataagtt taaaacgaat aacacgttag
      481 gtctcgtcta ggcaaagcat atttatgaag atgaattgaa cagacagaag taacatttta
      541 tatacatatg tgcatgggag cacgcttaaa gagaacagtg taacaaggta tatcaacata
      601 ccatggtgca gtgtctagtt aaactgaaac ctgcaatagt aaattttctg ccattatgta
      661 cagaatctac tattgtaggt ttttcttttt atacataagc attatgcgag agattagaaa
      721 taaggaggtt atgcagtgtt aagttttcaa ttgctatttt cactgtttgt tattgcgctt
      781 atcattgcat tgataagtgg cttgttgttt ttagcaccag ttatgccaat gagatatatt
      841 aaattacatt tatacatact agtcatgcca gtattatttg cagtcattgg ctttttcggt
      901 attcatggtc aacatgtctt aggtccattt aaaatagatc gtttatcttg gttattagct
      961 ggctttgtaa tggcgcttgg ctttattatt caaaagtttt caatgcgata tttactaggt
     1021 gatcatcatt atagacatta ctttccattg ttcactgcga ttacatcgtt tgcatcttta
     1081 gcatggatgt ctgaagactt aagactgatg gcactctgct ggggtatgac attattatgt
     1141 ttaacattgc tgatgaacgt taatcgtttt tggaaagtgc cacgtgagtc tgcgaaatta
     1201 tcaagcatga catttttatg tggttggctt gcattcgttg gagcaattgt atctatttat
     1261 attgcaactg gcgagtggcg ggtaccacaa catatagcgc atccgacatg gtcattgttg
     1321 acgaatgtac tacttgtatt agctgtcatg ataccggcag cacaatttcc ttttcatcga
     1381 tggttgattg aatctgtaac agcaccaacg ccagtatcgg caattatgca tgcaggaatt
     1441 gtgaatgcag gtggtgttat tctaactcgt tttgcgccga tatttgataa tggatttgcg
     1501 ttatcattat tacttatcct ttcaagcatt tctgtattgt taggatcggg tattagcttg
     1561 gttcaagttg actataaacg ccaattggtg ggctctacga tgagtcaaat gggctttatg
     1621 ttagttcaat gcgcacttgg tgtatattca gcagcgatta tacatttaat attgcacggt
     1681 atttttaaag caacattatt tttacaatca ggttctatcg tgaagcgatt caatattcca
     1741 aaacaagcat ctgctaaaga cgcttatggc tggattgtca tgggacgtgt attagctatt
     1801 atcgtggcat ttctgttttg gatgagtagt gacagaagtg catatgaagt gttaagtgca
     1861 ctcattctag catggtcatt acttgtatct tggaatcaaa tggtagcctt tagtaaaggg
     1921 cgtatggcac gtctggttgg catgattttg atttcaattg tgacatttat ctacatcatc
     1981 acacataatt atttttacga tgtattacaa aatataacaa cacatgcgac aacgccgcct
     2041 acaattagtg tcatcattag tgttgtcata ttaatatttg gtagtttatt aagtatctgg
     2101 gtagcgcgtc atcgatactc taagactttt gcggtattgt acgtgtggtt agttaatcta
     2161 ggagaagcac gctcgaaagc gatagaaagt catccgaatt atttgaagaa gtatttatag
     2221 gaggtgaaag gtatgacaac acagttaaat atcaattcag tcattgaaaa tgcgaaacgt
     2281 gttattacac cattatcacc gatttcgatt tttgcagcac gaaatccatg ggaaggatta
     2341 gaagcggata cgtttgaaga tgtcgcaaaa tggttacgtg atgttcgcga tgtggatatt
     2401 ttcccaaata aagcattaat agaaagtgct gtggcacgcg gtgaattaga cgaaagtgtc
     2461 tttaatcaac ttgttactga tatgctactt gaacatcact acaatatccc gcaacactac
     2521 atcaatcttt atattgataa cattaaaaca ttaaaagacg tacctgcatc atacatgaat
     2581 cattcaaatg ttgatgttgt tgctgatcta ctattagaaa aatcaaaacg tgatatggct
     2641 gaatcatatc atcactatga tgtacgtccg atgagtgatg caataataga tgaacaaggt
     2701 gagccactta gcgaacaagt gaatcgtcaa atgattaaat ggacgaaact ttatatcgat
     2761 caatttctat cgagttggac aatgccgaag cgtgagcaaa gtttttacca tgcatggttg
     2821 catttagcgc aacatgacca tagttttact aaagcacagc gccaagtgat taaaggctta
     2881 cccaatgatc ctaaaatgac gatagagtca gtattaaatc atttttcaat agcgcaggaa
     2941 gactaccaag cttatgttga aggacatctt ttggcgttac cgggttgggc aggtatatta
     3001 tactaccgct cacaacagca tcactttgaa caacatttgt taacggatta tttggccatt
     3061 cggttagttg tcgaacaatt gctagttggt gatgagttta agtcagtcac taaagattgt
     3121 gaaagcagat cggaaaattg gtttaagcaa actgttgcat catggtgtta ctacagtgat
     3181 atgcctagcg atgtattact acaacatgac gtcaatgaaa ttcaaacgtt tattcatttt
     3241 gcagcaacta tgaataaaaa tgtatttaaa aatttatggt tgattgcctg ggaaatgaca
     3301 tacgaatctc agttaaaaca aaaaattaaa gcaggtcatg aaagtgtggc gggcgcatta
     3361 gatgtaaacc aagtaaatgt tactgaaaat gacaacgcta atcagtcaca ttcagtatcg
     3421 ttaaatgcca cacaagcagt tgatgaaaat aatagcgagc taaatcaggt gggcacatca
     3481 acgaaagcgc aaattgcatt ttgtatagat gttcgttcag aaccatttcg tagacatatc
     3541 gaagcagcag ggccctttga aacgattggt attgcaggtt tttttggatt acctattcaa
     3601 aaagatgccg tagacgaaca attcaaacat gattcattac ctgtcatggt accgccggca
     3661 tatcgcatta aagaatttgc agaccgctac gatatgaatg tttatcgaca acagcaacag
     3721 acaatgtcat cgatgtttta cacatttaaa ttgatgaaaa ataatgttat gcctagtctg
     3781 ttattgcctg aattaagtgg gccattttta agcttgagta ccattgtcaa ttcgattatg
     3841 cctagaaaaa gtcgcgtgtc tttacaaaaa ataaaacaaa aatggttaaa aaagcctgaa
     3901 acaaagttaa cgattgatcg tgagtttgac agaacatcag acttacctgt tggatttact
     3961 gaacaagagc aaattgattt cgcgttacaa gcgttgaaat tgatggattt aaccgaagca
     4021 tttgcgccgt tcgttgtgtt agcaggtcat gctagtcatt ctcacaataa tccacatcac
     4081 gcatcacttg aatgtggggc ttgtggtggt gcatcaagcg gttttaatgc taagttatta
     4141 gcgatgatat gtaatcgtcc aaatgtcaga caaggattaa aacaagcagg cgtgtatatt
     4201 ccagagacta ctgtttttgc ggcagcagaa catcatacgt ctactgatac attggcatgg
     4261 gtatatgtgc cagacacatt atcagcttta gctctagatg catatgaatc attgaatgac
     4321 gtcatgccga tgatttctga acacgcgaat cgcgaacgtt tggacaaact gccaacgatt
     4381 ggtcgtgtga atcatccagt ggaagaagcg cagcggtttg caagtgattg gagtgaggta
     4441 cgtccagaat ggggcttggc taaaaatgca tcatttataa ttggacgacg ccaattaaca
     4501 aaaggcattg atttagaagg gcggacattt ttacacaatt atgattggcg taaagataaa
     4561 gatggcaaat tgttaaatac catcatttct ggtccggcgc ttgtggcaca atggattaat
     4621 ttacaatatt atgcgtcgac agttgcgccg catttttacg gaagtgggaa taaagcgaca
     4681 caaaccgtca cgtcaggtgt tggtgtcatg caaggtaatg cgagtgatct gatgtatggc
     4741 ttatcatggc aatctgttat ggctgctgat cgaacgatgt atcattcgcc aattcgtttg
     4801 cttgtcgttg ttcaggcacc cgattttgtt gtagctagac tactcgcgaa taatgagcat
     4861 ttcgctagga aggtgtctaa tcattggctg cgtttaatga gcgttaatga ggaagggcgt
     4921 tttaaaagtt ggatttaacg atgaaatgtt gcgatgctaa gtaactaata tatcatcata
     4981 tctacaatgt tctagcttct taaaatatac taaaaatgat aaacttaatg ttatagttga
     5041 tgtcgtgata acgatacaaa gatgaagtaa tcaattttaa tattattgga gttagtgata
     5101 tgaaaagaac gaaaggtgaa atcgaagctg aaatcagtaa agccattacg caatgggaaa
     5161 aagatttcct tggcagaggt tccttgtcgg ttaaatcaga cattttaaga gatatggtga
     5221 ttattagttt acaaggtatc ttaacgccag cagaatatcg tgtgtgtagt acgaatgaag
     5281 gattattaaa tattaaacga acacgttctg aattagttga atccggtgag caagatttga
     5341 atgatatcat ttttaaaatt acaggtatca aagtgatgag cttccatagt gatttaagta
     5401 cagttacagg tgaacgtatt atcgtattca aacttgagga taatttggaa aagcatattt
     5461 aagaaggtta ggtatgcttc tgcaggctta ttaaagctat agaagtatgc ttggttaaac
     5521 agcgcaacgc catatatttg ggtgtgctgt ttttctatta tatgatgtgt ttatcatgtg
     5581 tatgtatgca ttggtttgaa tatagcccgt gaaaggtgta tgtcataaaa tttgtgaatg
     5641 gtgacaagtc aacgcaagca tatttgttgc gagacatgcc cactatcctt ttacataaag
     5701 agtatatgtg tgacgtaggc atataatcga taaagtatac ctaaaaaatt aggtataatg
     5761 accattgttg caataagttt ttcggccttg aaaccgataa cgcatagtat aataggaata
     5821 aaatataata caaatatcca gtaaatcaaa tttgaaaacg atggatgtat aggggtaaag
     5881 aatctaacta atgtaatgat taacgctata tagacaaaaa ttactctatt gatacgccct
     5941 accccctctg tataataaat atagatcgta ctaaattgaa aatagcaaaa tattgttatt
     6001 tcaattatac aatgttttat ttgcaatata cataacaata tgaactttat aattttgata
     6061 ttgtcggtaa agtgaaattt gtggcacatt gatattatgg ataacatttg taaaatgaaa
     6121 atgatgatac gaggagattg taagatgata gataaaaaat taacatcacc gaaaatgaca
     6181 gtgcctttat ttttaatcgc gctgattgta tttataggta tgttttacag tgtagtgaca
     6241 aatcaagaat ggcttaaaaa tatagatatg ggatcattaa catggtttac agattatttc
     6301 ggtgagccac aacgccagta tgttaacaat ttatttaatt actatatgac gtttagtgcg
     6361 gaaattggag atgtcaaagg tgtcgtgttg atttccatta tcgtcacaat catactgttt
     6421 attaaacaga ggcatttagc ggtttggttt gtgacatatt tggtttcagg tgtcatcatg
     6481 aacaaattaa ttaaagatac tgttttacgt ccaagaccat ataatcattt agccgttgat
     6541 acaggctttt catttccgag tggacattct aacgccagca cattattata tttcgcctta
     6601 atgatcatta ttatttcagt tgctgctaag acaatgacaa aagtgttgag tgcgctggtt
     6661 atgggaatat tatggcttag catattattt tgtcgccttt attttcatgc gcattacttt
     6721 tcagatgtca ttggcggcac gtcactagca atcatttggg tagcgttatt cttaatggta
     6781 tacccatact ttattaatca tcgacgacaa cgcgtttagc aaacatcttt agacatagtg
     6841 gaggtatata gaaaaatgag aattaaaaca ccgagtccat cttatttaaa aggcacaaat
     6901 ggacatgcga tattattatt acattcattt acaggtacaa atcgggatgt gaagcatctt
     6961 gcagctgagt taaatgacca aggatttagt tgttatgcac cgaattatcc aggtcatggt
     7021 ttattattga aagatttcat aacatataat gtagatgatt ggtgggaaga agttgagaaa
     7081 gcttaccaat ttttagtcaa tgaaggttat gaatctatca gtgcaacggg tgtgtcttta
     7141 ggtggattaa tgacattaaa attggcgcaa cactatcctt tgaaacgtat cgctgtcatg
     7201 tcagcaccaa aggaaaagag tgatgatggc ttaatagaac atttagttta ttatagtcaa
     7261 cgcatgtcga atattttaaa tctagatcag caagcatcga gtgcgcaatt agcagcaatt
     7321 gatgattatg aaggtgaaat tacgaagttt cagcatttta ttgatgatat catgacaaat
     7381 ttaaatgttg ttaaaattcc agctaatata ttatttggtg gtaaagatgc gccatcctat
     7441 gaaacaagtg cacactttat ttatgaacat ttaggatcag tagacaaaga attaaatggt
     7501 ctgaaggatt ctcatcattt aatgacgcat ggagaaggca gagatatttt agaagaaaat
     7561 attattcgct ttttcaatgc tttaacataa ttgaagattt taaaaattaa cacttgagtg
     7621 acgatgttat tatatttgtc atttgagtgt tttttgtggc gtgatagtat agggaactta
     7681 gacaaagtta atttgtattg tgatgtcgtg gaaatattat gtagttatac ctgaagttga
     7741 tttgtattgt gatgttgtgg gaattttgtg ggcattgtac ctgaagttgt tatgtggcga
     7801 gatgttgggg atatggatgg tgttgtatgt ggcgagatgt tggcgcaagt atacagcgtt
     7861 gaaccagaaa ttttaatgta gcgtaatgac aacacgttac aagggatttc ctatattatg
     7921 ataaactcag gcgctaccgt attaatgaaa caattttgat gcaggtgtat tgcatgttat
     7981 ttacatacat gttagagtgt tacttaaaca cgtagacgct ttaacgcttt aaaggagatg
     8041 tgaatgatga aaagacaaca atcacaatgg aagtcatcaa ctggatttat tttagctagt
     8101 gcgggttctg caatcggtct tggtgccatg tggaaattcc catatatggc agggatctat
     8161 ggcggcggtg cctttctagc tatgttctta atattcacca tttttgttgg attaccatta
     8221 ctcattatgg aattcactgt tgggaaaatg ggacggacat atacaacaca aatatatagt
     8281 aaattaactg gtaaaaaatg gctcaatatc attggctgga acggtaattt ggcagtgttt
     8341 attttatttg gcttctatag tgttattggt ggttggattg tcatttacat cggacaagta
     8401 ttatggcaat tagttatatt tcaacgcatc aatcatctcc aagaaatgaa ttttgaagcg
     8461 gtaatatcaa atccttggtt aaccgttcta gggcaaggta tattcatatt cgctacgatg
     8521 attattgtca tgttaggtgt tgaaaaggga ttagaaaagg catcgaaagt tatgatgccg
     8581 ttgctgtttg tctttttaat catcattgtg attaagtctt taacattaga tggtgcctta
     8641 gaaggtgtga aatttatttt acaaccaaga gtgtcagaga ttactgctga tggaatcttg
     8701 tttgcgctag gtcaatcatt ctttacgtta tcattaggaa ctacaggtat gattacttat
     8761 gcgagttatg cctctaaaga catgacgatt aagtcatcag ctatttctat cgttgttatg
     8821 aatatctttg tatctgtatt ggcaggccta gctatatttc cggcgttaca tagttttggc
     8881 tatgagccac aagaagggcc tggattatta tttaaagtac tgccaatggt ctttagtcaa
     8941 atgcatctag gcacattatt ctatttggga ttcttagtgc tgttcttatt tgcggcttta
     9001 acgtcatcta tttctttatt agaattaaat gtttctaact tcacgaagaa tgacaataca
     9061 aaacgtaaaa aagtcgcagt gatcggtagt atattagtat ttatcattag tattccagca
     9121 accttatctt ttggtatctt aaaagatgta agattcggtg cgggaacgat ttttgataat
     9181 atggatttca tcgtttcgaa tgtattgatg ccattaggcg cattaggtac tacgcttgtc
     9241 gtaggacaat tattagataa aaagttatta caacaatatt ttggtaaaga tcgatttaga
     9301 ttattcagtg gttggtatta cttaattaag tatgcgatgc ctgtcgttat tattttagtc
     9361 tttatcgtgc aattatttag ttaatacata gaagcatctg acacacaagt atttgtgttg
     9421 ctcaggtgtt tttttgtttg gaattgaaaa ttttgtactg gttctgaatt aatcgaaact
     9481 tagataattg caaaataaca aaatgtcgtt tataattctt atcttaaaag taagaattaa
     9541 aattgagaca tgaagaaagg tttcattcat ttgatcggca tgtcagatga gggtgcatct
     9601 atgattactt atgatttaat tggcaataca ccattagtac tgttagaaca ttatagtgat
     9661 gataaagtta aaatttatgc caagcttgaa caatggaatc ctggaggcag tgttaaagac
     9721 agactcggga aatatttagt agagatggca attcaagaag ggcgtgtgcg tgcaggtcaa
     9781 actattgttg aagcgactgc tggcaataca ggcatagggt tagctattgc agcaaataga
     9841 catcatttga aatgtaagat ctttgcgccg tatggttttt cagaagaaaa gattaatatt
     9901 atgatagcgc ttggtgcaga tgtttcaaga acgagtcagt ctgaaggtat gcatggggca
     9961 caattagctg cacgttcata tgctgaaaaa tatggtgccg tttatatgaa tcaatttgaa
    10021 tccgaacata atccggatac atattttcat acattgggac ccgaattgac ttcagcatta
    10081 cagcatattg attattttgt ggctggtatt ggctctggcg gtacatatac aggtaccgca
    10141 cgttatttaa agcaacatca cgtgcaatgt tatgccgttg agccagaagg gtccgtgtta
    10201 aatggagggc cagctcatgc acatgacact gaaggtatcg gttctgagaa atggccgata
    10261 tttttagaga gaagtcttgt agatgggata tttacgatta aagatcaaga tgcctttcga
    10321 aatgtcaaaa gtttggcaaa gcatgaagga ttattggtag gcagttcttc aggtgcagca
    10381 ttacaaggtg cattgaattt aaaagcgcaa ttatctgaag gtacgattgt tgtcgtattt
    10441 ccagatggta gcgatcgata tatgtctaag gaaatattta attatgagga gaatgattat
    10501 gaataagaaa acgaaattaa ttcacggtgg acacacaacg gacgactata caggtgcagt
    10561 tacaacacca atttatcaaa caagtacata tttacaagat gatattggtg atttacgtca
    10621 aggatatgaa tattctcgta ctgcgaatcc aacaagaagt tctgtagaaa gcgttattgc
    10681 gtcattagaa aatggcaaac atggctttgc atttagttca ggtgttgcag caatcagtgc
    10741 agttgttatg ctgttggaca aaggtgatca tattatttta aattcagatg tatacggcgg
    10801 tacttatcgc gcattgacaa aagtatttac acgatttggc gttgaagtgg attttgtaga
    10861 tacaacgcat acagattcaa ttgtacaagc gatacgccca acaacaaaaa tgttatttat
    10921 tgaaacacct tctaatccat tattacgtgt tactgacatt aaaaagtctg ctgaaattgc
    10981 gaaagaacac ggtttgattt cagtcgttga taacacattt atgacacttt attatcagaa
    11041 tccattagat ttaggcatcg atattgtctt acattctgca acgaaatatt taggtggaca
    11101 tagtgatgtc gttgctggtt tagttgcaac atcggatgac aagcttgcag aacgtttagc
    11161 atttatttca aattcaacag gtggcatttt aggacctcaa gatagttatt tacttgtgag
    11221 ggggattaaa acattaggtt tacgtatgga acaaattaat cgcagtgtta ttgaaattat
    11281 taaaatgtta caagcacatc cagctgtgca acaagtgttc catccaagta ttgaaagtca
    11341 tttaaatcat gatgtccata tggctcaagc ggatggccat acaggtgtga ttgcttttga
    11401 agtgaaaaat acagaaagtg ccaaacaatt gattaaagca acatcgtatt acacattagc
    11461 tgaaagttta ggtgcagtgg aaagtttaat ttcagtacct gcattgatga cacatgcatc
    11521 tattccagca gatattcgag ctaaagaagg tattacagac ggacttgtaa gaatttctgt
    11581 aggtattgaa gatactgaag atttagtaga tgatttaaaa caagcactag atacgttata
    11641 aataatagca gcactggcat atattttgag ttcaacgttt gttgttcttg aatatgctag
    11701 tgcttttttg tgtggtgtta atatttcgta attgcattgt ggaggattgc tggtcatttt
    11761 tattcattta atttgataaa tgtcatagta gtctcacaat tcgtcattgt cacaaggtat
    11821 tattttttaa ttttttagaa aattcggcaa attgtattga cgatatatgc aattttacat
    11881 ataatgctct tattcctagt ggattaataa gaatttgtag gaggggcgat gatgattgag
    11941 tttcgacaag ttagtaaatc atttcataag aaaaagcaaa caatagatgc tttgaaggac
    12001 gtatcattta cggtcaatcg caatgatatt tttggtgtga ttggatatag tggtgcagga
    12061 aaaagtacgt tggtaagact cgtgaatcat cttgaagctg cctcgaatgg acaagtgatt
    12121 gtagatggac atgatattac gaattatagc gataaaatga tgagggatat taagaaagat
    12181 atcggtatga tatttcagca ttttaattta ctaaattcag ctacggtatt taaaaatgta
    12241 gctatgccgc tcattttaag taagaaaagc aaaacagaaa ttaagcaacg agtaacggaa
    12301 atgcttgaat ttgtaggatt gagtgataaa aaagaccaat ttcctgatga attatctggt
    12361 ggtcagaagc aaagggtggc tattgcaaga gcgcttgtta ctaatccgaa aatactccta
    12421 tgcgatgaag caacaagcgc attggatcca gcaacgactg cttcgatatt gacgttatta
    12481 aagaatgtca atcaaacctt tggcattaca attatgatga ttacacatga aatgcgcgtt
    12541 attaaagaca tttgtaatcg tgttgctgta atggaaaagg ggcaagtggt tgaaacagga
    12601 actgttaaag aggtgtttag tcatcctaaa acgacgattg ctcaaaattt tgtgtctaca
    12661 gttatacaga ctgagccaag tccatcattg attcgtcgat tgaatgacaa acaagttggc
    12721 gattttaaag attataaaat cttcgtcgag gaaactcaga tgacacaacc gattataaat
    12781 gacttgattc aaatttgtgg cagagaggtt aaaattttat tttcatccat gtcagaaata
    12841 caaggtaaca ccgtatgtta tatgtggctt cgatttaata tggatcaaca atttgatggc
    12901 acggcaataa atcaatattt caaagagaaa aatattcaat ttgaggaggt gcattaacat
    12961 atgtttggtt ctgatttaga cagtgcacag ttattacaag cattatacga aacactatat
    13021 atggtatcta ttgctttatt tttaggagca gtgattggta ttccattagg tgtcttattg
    13081 gtaattactc gaaaacaagg catatggccc aatatagtga tacatcaagt tttaaatcct
    13141 ataatcaata ttttaaggtc actaccattt attattttgt tgattgcgat tgtgccattc
    13201 acaaaattag tagtaggtac ttccattggt acgactgctg ccatcgtgcc tttaacagta
    13261 tatgtggcac cttacattgc aagacttgtt gaaaactcat tattggaagt agacgagggg
    13321 attattgaag cggcgaaagc gatgggcgct tcaccactac agatcattag atatttttta
    13381 attcctgaag cgttaggttc attagtatta gcaattacca ctgcgattat tggacttatt
    13441 ggaagtacgg cgatggcagg cgctgttggc ggtggtggta taggagactt agctttagtg
    13501 tatggttatc aaagatttga tacgacggtc attattatta ccgttattgt attagtcgtt
    13561 attgtccaag tgattcaaac gctagggaat gtcctagcta gattcatacg tagacattga
    13621 tgatatatag tgaagatttt gaaaggaatt gatagaatga aaagattgat tgggttagtt
    13681 atcgtagcac ttgtattatt agcagcgtgt ggtagtaaca atgataaaaa agtaacaatt
    13741 ggtgtcgcgt caaatgacac taaggcttgg gagaaggtta aagaattagc taaaaaagat
    13801 gatattgatg tggagattaa gcacttctcc gattacaatt taccgaataa agcattaaac
    13861 gatggtgata ttgatatgaa tgcattccaa cattttgcat ttttagatca atataaaaag
    13921 gcgcataaag gaacaaagat ttcagcatta agtacaacag ttttggcacc gttgggcgtt
    13981 tactcagata aaattaaaga tgtcaaaaag gttaaagatg gtgctaaagt tgtcattcca
    14041 aatgatgtgt caaaccaagc acgtgcactt aaactattag aagcagctgg tttaataaaa
    14101 ctgaaaaaag atttcggatt agcaggtaca gtgaaagata taacgtcaaa tccaaaacat
    14161 ttaaaaatta ctgcagtaga tgcacaacaa actgcacgtg ctttatctga tgtcgatatt
    14221 gcagttatta ataacggtgt agcaactaaa gctgggaaag atccaaaaaa tgatccgatt
    14281 ttcttggaaa aatcagattc agatgctgta aagccatata ttaatattgt tgcagttaac
    14341 gacaaagact tggataacaa aacatatgct aaaatcgtag aattgtatca ttcaaaagaa
    14401 gctcaaaaag cgttgcagga agatgtcaaa gatggagaga aacctgttaa tttatctaaa
    14461 gatgagatta aggcaataga aacgtcatta gcaaaataaa ttatattacg tcctacaagc
    14521 atagttcatg cttaagtttg tagggcgttt ttgtttgcct ataaaaatgt ttcaaaagac
    14581 agaaagggat tgtaatcatt ttatagtgaa atattatgaa attgtaataa tttagatatt
    14641 gtaaaatcta ataagttgta ataattttaa gaggtaatta taaaatttga tgacacagta
    14701 tatgattttt ttgtaatcat aatgtcatca aacatcaacc tattatacat aattaaatcg
    14761 tataatgatg tagtattcat aaattcggat aaaaagaatg ttaggaaagt tatgcaagag
    14821 gaggatttta aagtgcaaaa aaaagtaatt gcagctatta ttgggacaag cgcgattagc
    14881 gctgttgcgg caactcaagc aaatgcggct acaactcaca cagtaaaacc gggtgaatca
    14941 gtgtgggcaa tttcaaataa gtatgggatt tcgattgcta aattaaagtc attaaacaat
    15001 ttaacatcta atctaatttt cccaaatcaa gtattaaaag tatctggctc aagtaattct
    15061 acgagcaata gtagccgtcc atcaacaaat tcaggtggcg gatcatacta cacagtacaa
    15121 gcaggcgact cattatcatt aattgcatct aaatatggta caacttacca aaacattatg
    15181 cgacttaatg gtttaaataa tttctttatt tatccaggtc aaaaattaaa agtatcaggt
    15241 actgcaagct caagtaattc tacgagcaat agtagccgtc catcaacgaa ttcaagcggt
    15301 ggatcatatt acacagtaca agcaggtgac tcattgtcat taatcgcttc aaaatatggt
    15361 acaacatatc aaaacattat gagacttaat ggcttaaata atttctttat ttatccgggt
    15421 caaaaattga aagtaactgg taatgcatct acgaactcag gatctactac aacgacaaat
    15481 agaggttaca atacaccagt attcaatcac caaaacttat atacatgggg tcaatgtaca
    15541 tatcatgtat ttaatcgtcg tgctgaaatt ggtaaaggta ttagcactta ttggtggaat
    15601 gcaaataact gggataacgc agcggctgca gatggttaca caattgacaa tagacctact
    15661 gtaggttcaa tcgctcaaac agatgtaggt tattatggtc atgttatgtt tgtagaacgc
    15721 gtaaataacg atggtagtat tttagtttca gaaatgaact attcagctgc accaggtatt
    15781 ttaacttaca gaacggtacc agcttaccaa gtaaataatt atagatatat tcactaaagt
    15841 cttacgtata taaatatata taatgaattc ctattacatt tcacaagctg aaaagttgga
    15901 tttaatccta aactttatgg cgtgactatc aaagtcgtga ttattgtaat aggaattttt
    15961 gtataagaaa aaggattgat cgaatgaacg tcatgctcta tgttcgacca atccttttct
    16021 gatttagtat tcatgcttta ctttgcgaat gcttgtaaat aatctagcac cgtttgttat
    16081 taaagtaaca actgccactg cttttttgaa cttgcgtggt gacttaattg aaatgtaaaa
    16141 gtctaacacg gttcctacag tgcttaaacc taatgaaaga tatactaatt ttttattttt
    16201 agcatgatat ttatagccat tgtagccgtc gactatgaaa cctgcgacat ttagtaaact
    16261 tgataaacgt tgtgatttgg aacgttttgc cataataata attcccccta gtatcttctg
    16321 tttatatgga ataattgggt gcgctgaatt gctaacgttt tgcgctataa ctactcatat
    16381 atgataacat aattgtacag tataatttga aaaattgatt tcacaaagtt ggggtgtcaa
    16441 agatgattaa atgtgtctgt ttagtagaag aaacagctga taaaatattg cttgttcaag
    16501 taaggaatcg cgaaaagtat tatttcccag gtggtaaaat agaagaaggg gaatcaccag
    16561 tacacgcgct gttaagagaa gtaaaagaag aattaaattt aacattaaca atggatgaaa
    16621 ttgaatatat cgggacaatt gtaggtcctg catatccaca acaggatatg ttaactgagt
    16681 taaatggatt tcgcgcatta accaaaatcg attgggaaaa cgtaactatc aataatgaaa
    16741 ttacggatat acgctggatt gataaagata atgatgcgtt gattgcgcct gctgtcaaag
    16801 tttggattga aacgtatgat ggtaaacatg acaaataatg acaccatcat gttacgacat
    16861 tatgtcccac aagattattc gatgttagaa gcttttcaat taagtgaaag tgatttgaag
    16921 tttgttaaaa cgccagagga aaatattaca gctgcaatgt ctgataatga aaggtatccc
    16981 atcgttgtaa tggatggcag gcaatgtgtg gcctttttta cattacatcg tggaaaaggt
    17041 gtcgcaccat ttagcgataa ccaagatgca gtatttttca ggtcatttag tgttgatcaa
    17101 cgttatcgta aaagaggaat aggtaaagtg gtaatggaaa aattggcgtc atttatcact
    17161 tcaacatttc aggatattaa tgagattgtg ttaacggtta atactgacaa tccacatgcc
    17221 atggcacttt atcgccaaca aggatatcaa tatatgggag atagtatgtt cgtcggaaga
    17281 cctgttcata ttatggcgct aactataaaa taaattaaat ttaaaagcat ctttactcat
    17341 cgtcgaccac aacaattaat gatgaataaa ggtgcttttt gttatagacc accggacaat
    17401 ttactatagt aaaaagcgac ctagggaaca attgacatat atccacaggt cgcttacctt
    17461 aagttatatt gctagttgcg attaattgat agactcatca tttttgcgct gtcgagatgg
    17521 tctttttatt aaaaatgccg taatccaagc cgtaatcgga atactgattg caacggcaat
    17581 accgcctaaa ataatagaaa taaattcttg ggcaaatatt ttcgagttta taatatgacc
    17641 aaatgaatat ttaagtttga aaaaccaaat aaataaagca agttggccac caaaaaaggc
    17701 aaggtaaatc gtgttcgcag atgtcgctaa aatttctcta ccaacacgca tgccagattg
    17761 gaataattcg tattgcgtaa gtgttggatt cacttgatgc aattcataaa tgggtgaact
    17821 aatggtaatc gttaaatcta tcacagctgc aataacagca agaataatag tgaacaccat
    17881 aaattgaatc atatcaatgc caatattcat tgaatacaca tatgtttcat cttgttgttc
    17941 ggttgaaaag ccttgtagat gaccgaagta gaccgataaa taaatgagtg taatcaacaa
    18001 tattgttgta acgatagtgc tgataaatgc agcttgtgtt ttaacattgt aactattgag
    18061 tacgaataaa ttacaagcgc caataataat gcagaaaaag aatgtgacga cataaatcgg
    18121 tacgccaaaa ataatcaata caatactaat aattaaaata gcgaaattta aaaatagggt
    18181 taaataagag atgaatccct ttttacctcc gaaaattatc atcagaaaga ggagcaataa
    18241 cgccaatata aatacagcat tcattgtttc gccctcctta atgtttcaaa tatttccata
    18301 aacaatattg tgataggaat tgtaagtacg atacctatac cgcctgttag tgcgcgcgca
    18361 atttctaacg accaattcat cgaaatagta taagtcactg tattggcatt ttttaaaaag
    18421 attaaaaaca taggtagtgc accggataaa tatgagaata ataagatgtt agtcattgtt
    18481 cccataatat cttggcctat gtttcgccca gcaagcgccc atctcctcat tgaaatgtgc
    18541 ggcgtacgct gtaaaatttc atgcatacca ctagcaattg taattgcaac atccataata
    18601 gcgccaagtg aacctattaa cactgaggct aggaagatat ctttcggtgg taatgataaa
    18661 aagttcatcg tttcatattt aatgccttta ccatctgtca tatatatgat taattctgtt
    18721 aaacctatac tcaaaaaagt tccgataatt gtactggcta tggtaatgag tgtacgcata
    18781 tgccagcctg taacgagcaa taaagtgagt attgttgaac agatcatggc aatggtcatg
    18841 agtaagaata aattaatatt gctatgttga atatgaatgt aaattgcgat taatatggca
    18901 atagaattca agattaatga taaaatcgat tgcagtccga ctttgcgacc aaccaataat
    18961 acagttaata agaacaaacc agtgatgata accgttaagg tatcacgctt cttttctata
    19021 atataagcat cactcggctt gttagaaata tgtaataata ctttttcgtg tgtgcgaaat
    19081 gcctcagaat ctgcttgcga tttgacgtac tgatgattaa tcgtcgtcgt ttctccagca
    19141 aattgaccat ttaatatttt gacttttaat tgatttttat atttaatatc acgattattt
    19201 tgtgcatctt ttgtaggtgt cgaagaaaca tgtttgacat ctataatttg accaattggt
    19261 ttgttgtaaa agttctcatt attgaatgtg aataaaatag caccaatgaa tgcgatgcag
    19321 aacaaaccta aaattatatt aaatggcttt gtaaataaat ttctatattt caaaaacaaa
    19381 accccaattc tatgaatgaa ttaatatggt gattatacgc ccttaatttt ttattttcaa
    19441 agatattact gtgaaatgta taacgaaaat catcattgat agagtcgaat ggcttaatgg
    19501 aatgtagacg ttttagtcat taattgttga atgagtgtta ataagatacc aatatcactc
    19561 ttcgtataag gttctttcgt aatagcacat atcgttcttt ttaattcagt atggtctaat
    19621 tttatatcta tacatgattt agattctggt aaatgtatat tttgtgatga aatgatgtaa
    19681 ccttcttttt gacgaaggag atactgcgca agtggttggc tactgattgt gtatacatct
    19741 gatttagtaa tcttacgcaa ttgttttttt acagattcgg caaatggtcc caagcaataa
    19801 atatgactat gctcaaactg aattaatggt gggtgtgtcg ccatcgtgat tggatcgtct
    19861 gaaggcgcat ataaatgata gtgctcttcg aataaaggta gcatatgtaa ttgtttgtgt
    19921 ttacgtattt ctggtgtaag ttccgtgaaa ccaatgtcta tatttccatt taatacgcta
    19981 tttataattg tgtcatgttc taataaatta gggatgacat gtgtatcatt ttgtaaatga
    20041 aacgtttgga taagtggtag taacatgtgg gatacgtcac tctcattata gccaatgtag
    20101 atgcttttat ttttagttaa tccatgactt tgaaattgtt caatcgtgct atctaaatgt
    20161 tcaataatac gcagagcttc attaaataat aatttccctt cagatgtgag cgtaatatta
    20221 cgtccttgct ttttaaataa tgacacatta agttcttgtt ctaataatgt aatttgacgg
    20281 cttatcgctg attgagcaat gtttagttca agtgctgttt cggagatatg ttctctttta
    20341 gcgacctcga taaaatatct taattgttta atttccatag cgatataggc acctccaaaa
    20401 atgagtgttt tgtaactatt atagcaatat tattgataaa tgttctattt tttagatgaa
    20461 tatcttctat tttatatatt gaacagataa attttttaga ttatagtaat tatcattaat
    20521 aactaatatc agaatattct aaaaaagggg tgtgcatcat gcacaatgag aaattaatta
    20581 aaggcttata tgactatcgt gaggaacatg atgcgtgtgg tattggtttt tatgcgaata
    20641 tggacaataa aaggtctcac gacatcattg ataaatcgct tgaaatgttg cgacgtttag
    20701 atcacagggg cggggtcggc gcagatggca tcactggtga tggcgcaggt attatgactg
    20761 aaataccttt tgcatttttc aaacaacatg taacggactt tgatatccca ggtgaaggtg
    20821 aatatgccgt ggggttattt ttttccaaag aacgcatttt aggttctgaa catgaagtag
    20881 tttttaaaaa atattttgaa ggcgaagggt tatcaattct tggttatcgt aatgtaccag
    20941 ttaataaaga tgccattgct aaacatgtag cagatacgat gccagtcatt caacaagtgt
    21001 ttattgatat tagggacatt gaagatgttg aaaagcattt gtttttagcg agaaaacaat
    21061 tagagttcta ttcgactcag tgcgatttag aattgtattt tacgagctta tcacgcaaaa
    21121 caattgtata taaaggttgg ttacgatcag accaaattaa aaaattctat acagatttat
    21181 cggatgattt atatcaatca aagctagggt tagtgcattc gagatttagt acgaatacat
    21241 tcccgagttg gaaaagggcg catcctaacc gtatgttaat gcataatggt gagattaaca
    21301 cgattaaagg taatgtgaac tggatgcgag cacgtcaaca taaattaatc gaaacattat
    21361 ttggtgagga tcaacataaa gtgtttcaaa ttgtcgatga ggatggtagt gactctgcta
    21421 ttgtagataa tgcgctagag ttcttatcgt tagccatgga gccagaaaag gcagcgatgc
    21481 tactcatacc tgaaccttgg ttatataatg aagcgaatga tgcaaatgta cgtgcgtttt
    21541 atgaatttta tagttattta atggagccgt gggatggtcc tacaatgatt tcgttctgta
    21601 acggtgacaa acttggcgcg cttacagata gaaatggatt acgtccaggt cgttatacga
    21661 ttactaaaga taactttatt gtcttttcat ctgaagtggg tgttgtggac gtacctgaaa
    21721 gtaatgttgc ttttaaaggt caattgaatc ctggaaagtt attgcttgtt gattttaaac
    21781 agaataaagt cattgaaaat aatgatttaa aaggtgcgat tgccggagaa ttaccatata
    21841 aagcgtggat tgataaccat aaagttgatt ttgattttga aaatatacag tatcaagatt
    21901 cgcaatggaa agatgagacg ttatttaaat tacaacgtca atttgcatac acgaaagaag
    21961 agattcataa gtatattcag gaacttgtag aaggtaagaa ggatcctatc ggtgcaatgg
    22021 gatatgatgc gccaattgca gtgttgaacg agcgaccaga atcactattt aattacttta
    22081 aacagctgtt tgcacaagtt acgaatccac caattgatgc gtatcgtgaa aaaatcgtaa
    22141 cgagtgaact ttcttattta ggtggcgaag gtaacttact agcacctgac gaaacggttt
    22201 tagatcgtat tcaattgaaa agaccggtat tgaatgaatc acacttagca gcgattgatc
    22261 aggagcattt taaattaaca tatttatcaa cggtatatga agggaatttg gaagatgcgt
    22321 tagaagtatt aggccgagaa gcagtggatg ctgtaaagca aggcgctcaa attctagtgt
    22381 tagatgatag tggattagtt gatggcaatg gctttgcaat gccgatgtta ctcgcaataa
    22441 gtcatgtgca tcaattactt attaaagcag atttacgtat gtctacaagt ttagtcgcta
    22501 aatctggtga gacacgagaa gtgcatcatg ttgcttgttt actcgcatat ggagcgaatg
    22561 caattgtgcc atacctagcg caacgtacag ttgaacaact gacattgaca gaagggttac
    22621 aaggcaccgt tgtcgataat gttaagacat atacggatgt attgtcggaa ggtgttatta
    22681 aagtaatggc gaagatggga atttcgacag tgcaaagtta tcaaggtgca caaatatttg
    22741 aagcgattgg cttgtctcat gatgtgattg atcgttattt tactggaaca cagtctaagt
    22801 tatctggtat ttcgattgat caaattgatg ctgaaaataa agaacgtcaa caaagtgatg
    22861 ataattatct tgcatcaggt agtacattcc aatggagaca acaaggtcaa catcatgctt
    22921 ttaatccgga atctattttc ttattgcagc acgcatgtaa agaaaatgac tatgcgcaat
    22981 ttaaagcata ctctgaagcg gtaaacaaaa atagaacaga tcacattaga catttacttg
    23041 aatttaaagc atgtacaccg attgacatcg accaagttga accggtaagt gacattgtca
    23101 aacgttttaa tacaggggcg atgagttatg gatcgatttc agcggaagca catgaaacgt
    23161 tagcacaagc catgaaccaa ttaggtggaa agagtaatag tggtgaaggt ggcgaagatg
    23221 caaaacgtta tgaagtacaa gttgatggaa gcaacaaagt aagtgcgatt aaacaagttg
    23281 cttctggacg ttttggtgta actagtgatt atttacaaca tgccaaagaa attcaaatta
    23341 aagttgcgca aggtgcaaag cctggtgaag gtggtcaatt acctggtact aaggtatatc
    23401 cgtggattgc gaagacaaga gggtcaacgc caggtatcgg tctgatttca ccaccgccac
    23461 atcatgatat ttattcaata gaagatttag cgcaactgat acatgatttg aaaaatgcga
    23521 ataaagatgc agatatcgcg gtaaaattag tttcgaaaac aggtgttggt accattgcat
    23581 ctggggtggc gaaagcattt gcagataaaa ttgtcatcag tggttacgat ggtggtacag
    23641 gggcttcacc taaaacgagt atacagcatg ccggtgttcc ttgggagatt ggtttagcag
    23701 aaacacatca aacattaaaa ctaaatgact taagaagtcg tgttaagtta gaaacagacg
    23761 gtaagttatt aactggtaaa gatgtagcgt acgcatgtgc gcttggagcg gaagaatttg
    23821 gatttgcaac tgcaccatta gtggtgttgg gctgtattat gatgcgtgta tgccataaag
    23881 atacatgtcc agtaggagtt gcaactcaaa acaaagattt acgtgcttta tatagaggta
    23941 aagcacatca tgttgttaat tttatgcatt ttattgcaca agaattaaga gaaattttag
    24001 catctttagg tttgaaacgt gtagaagact tagttggaag aactgattta ttacaacgat
    24061 catcaacatt aaaagcgaat agcaaagcgg ctagtattga tgttgaaaaa ctgttatgtc
    24121 ctttcgatgg gccaaacaca aaagaaattc aacaaaatca taatcttgag catggatttg
    24181 atttaacaaa tttatatgag gtaacgaagc catatattgc tgaagggcgt cgctatacag
    24241 gtagctttac agtaaataat gaacaacgtg atgtaggggt tattacaggt agtgagattt
    24301 cgaaacaata tggagaagca ggacttcctg aaaatacaat taatgtttat acgaatggtc
    24361 atgctggtca aagtcttgca gcatatgcac cgaaaggctt aatgattcat catactggag
    24421 atgcgaatga ctatgttggt aaaggattat ctggtggtac ggtcattgtc aaagcacctt
    24481 ttgaagaacg acaaaatgaa attattgctg gtaacgtctc attctatggt gcgacaggtg
    24541 gtaaggcatt tattaacggt agtgcaggag aaagattctg tattagaaat agtggtgtag
    24601 atgttgtcgt tgaaggtatc ggcgaccatg gattagagta catgactggt ggacatgtca
    24661 ttaatttagg tgatgtaggt aagaacttcg gtcaaggtat gagtggtggt attgcttacg
    24721 ttatcccgtc tgatgtagaa gcttttgttg aaaataatca actagatacg ctttcgttta
    24781 caaagattaa acaccaagaa gaaaaagcat tcattaagca aatgctggaa gaacatgtgt
    24841 cacatacgaa tagtacgaga gcgattcatg tgttaaaaca ctttgatcgc attgaagatg
    24901 tcgtcgttaa agttattcct aaagattatc aattaatgat gcaaaaaatt catttgcaca
    24961 aatcattaca tgacaatgaa gatgaagcga tgttagctgc attttacgat gacagtaaaa
    25021 caatcgacgc taaacataaa ccagccgttg tgtattaagg aaagggggag atacgatggg
    25081 tgaatttaaa ggatttatga agtatgacaa acagtactta ggtgaattat cactggtaga
    25141 ccgtttgaag catcataaag catatcaaca acgatttact aaagaagatg cctctatcca
    25201 aggtgcgcga tgtatggatt gtggaacgcc gttttgtcaa accggacaac agtatggtag
    25261 ggaaacaata ggttgtccaa ttggaaacta cattcctgaa tggaacgact ttgtgtatca
    25321 tcaagatttt aaaactgctt atgaacgctt aagcgaaaca aataactttc ctgactttac
    25381 agggcgtgta tgtcctgcac catgcgaaag tgcttgtgtg atgaagatta atagagaatc
    25441 gattgcgatt aaaggtattg aacgcacaat tattgatgaa gcttttgaaa atggttgggt
    25501 agcgccgaaa gttccgagtc gccgtagaga tgaaaaagtg gcaatcgttg gaagcggtcc
    25561 agcaggatta actgctgctg aagaacttaa tctactagga tatcaagtga ctatttatga
    25621 acgtgctaga gaatcaggcg gtttattaat gtatggtata cctaatatga agcttgataa
    25681 agatgtggtt cgacgtcgta ttaagttaat ggaagaagcg ggcattactt tcgttaatgg
    25741 tgttgaagta ggcgttgata ttgataaagc aacgttagaa tctgagtatg atgccattat
    25801 attatgtact ggtgcacaaa aaggtagaga tttaccttta gaaggacgca tgggtgatgg
    25861 tatacatttc gctatggatt atttaactga acaaacgcaa ctgttaaatg gagaaattga
    25921 tgatataaca ataactgcaa aagataagaa tgtcattatc attggtgctg gtgatacagg
    25981 ggcagactgt gtagcgacag cattaagaga aaattgtaag tcgattgttc aatttaataa
    26041 atatacgaaa ttgccagaag caattacgtt tacagaaaat gcatcatggc ctttagcaat
    26101 gccggtgttt aaaatggact atgcgcacca agagtacgaa gctaagtttg gtaaggaacc
    26161 acgtgcatat ggtgttcaaa caatgcgtta cgatgttgac gataaaggac atatacgtgg
    26221 tttgtatact caaattttag agcaaggcga aaatggtatg gtcatgaaag aaggacctga
    26281 aagattttgg cctgctgacc ttgtattatt atcaatcggc ttcgaaggta cagaaccaac
    26341 agtaccgaat gcttttaaca ttaaaacgga tagaaatcga atcgtggctg atgatacaaa
    26401 ctatcaaact aataatgaaa aagtatttgc tgctggagat gctagacgtg gtcaaagttt
    26461 agttgtatgg gcgattaaag aaggtagagg cgtagcgaaa gcagtagatc agtatttagc
    26521 gagtaaagtt tgtgtataat ctttgtgtga caatgatgat gttcgcgttg acgttgtatg
    26581 gtgctgaatt gaaaaggaag cgacttagta tctatcaacg tcacatgcca tctttgtaac
    26641 ctaaaaataa aggtttgtaa gacaactaat agattaatta taagtagtga ttttttacat
    26701 tcgttttata ggtcaactgt agtggaagac aatgatttgt ggtaatcatg taatgcttaa
    26761 aaacaatatt gacttttaca gaacgttcat atatgataaa tattgtgttt aggaggaata
    26821 cccaagtccg gctgaaggga tcggtcttga aaaccgacag gggcttaacg gctcgcgggg
    26881 gttcgaatcc ctcttcctcc gccatcaata tttatattaa atactatata taatgaaggt
    26941 aagtgctcaa attttgagta tttacctttt ttatttgtct ttgaatgggt cgtatttttg
    27001 gctatagaaa tgataaggca ttgaaataga aaggacattt ggcttttgaa tgacacatgg
    27061 ctgaatgtct tttttatttg gtgatgtacg taggagtctt tgcttgggca tggaaattac
    27121 tatttgggta tgggtatgat aatacagctt attctttaac atagtgtatg cgtttggagg
    27181 ttatggataa tgctattgta gcgaaaattc atgctataaa tgtagtcgta gccatttgat
    27241 tttcttaaat atctaaacta gataattgtg aaatattaaa atattttcat gaaagccttt
    27301 acataacttg tctagacaag ttatactctt tttaagacat taagggagtg aagttcatgg
    27361 ctgtaaaaag agaagatgta aaagccatcg ttaccgcaat tgggggaaag gataatctag
    27421 aagctgcaac acattgtgta acaagattgc gtttagtact taaagatgaa agtaaagttg
    27481 ataaggaagc gttaagtaat aatgctttag ttaaagggca gttcaaagca gaccatcaat
    27541 atcaaattgt cattggtcca ggaacagtcg atgaagtgta taagcagttt attgatgaaa
    27601 caggtgctca agaagcttct aaagatgaag cgaaaaaagc agctgcgcaa aaagggaatc
    27661 cagtacaacg tttgattaaa ttgttggggg atatttttat accaatatta cctgcgattg
    27721 tgacagctgg tttgttaatg gggatcaata atttacttac aatgaaagga ttatttggtc
    27781 cgaaagaact cattgtgatg tatccacaaa tagctgatat ttcaaatatg ataaacgtca
    27841 tagcaaacac ggcatttatt ttcttaccag cattaattgg ttggagtagt atgcgtgtat
    27901 ttggtggtag tccgattcta ggtttagtct taggtttgat tttaatgcac ccgcaattag
    27961 tatctcagta tgatttggca aaaggtaata ttccgacgtg gaatttattt ggcttagaga
    28021 ttaagcagtt gaattaccaa ggtcaagtgt tgcctgtttt aattgcagct tatgttctag
    28081 ctaaaattga aaaaggatta aataaagttg ttcacgattc gataaaaatg ttggtcgttg
    28141 gacccgtagc gcttttagtt actggatttt tagcatttat tatcattgga ccagttgcat
    28201 tattgattgg tacaggtatt acatctggtg ttacatttat attccaacat gctggttggt
    28261 taggtggcgc aatttatggt ttgctatatg caccgcttgt aattactggc ttgcatcata
    28321 tgtttttagc agtagatttc caattaatgg gtagcagttt aggcggtaca tacttatggc
    28381 caattgttgc aatctcaaat atttgtcaag gttcagcagc atttggtgca tggtttgtct
    28441 ataaacgacg taaaatggtt aaagaagaag gtttagcatt aacatcttgt atttctggta
    28501 tgttaggtgt tacggagcca gccatgtttg gtgtgaactt acctttaaaa tatccattta
    28561 tcgccgcaat atcaacgtct tgtgtattag gggcaatcgt tggtatgaat aatgtacttg
    28621 gaaaagttgg tgttggtggt gtaccggcat tcatttcaat tcaaaaagaa ttttggcctg
    28681 tatatcttgt tgtaacagga attgcaattg ttgtaccatg tatattaacg attgtgatgt
    28741 ctaattttag taaacaaaaa gcaaaagaaa ttgttgaaga ttaataaaat aaaaaagggg
    28801 cgttcgttat ttggacgcct tttattatgt tataaggtgg tcatcgtgtg ttgaaagaaa
    28861 tagattggag aaagtcagta gtatatcaaa tatatcctaa gtcatttaat gatacgacag
    28921 gaaatggtat aggtgatatc aacggtatta tagaaaaatt agattatatc aaattgttag
    28981 gtgttgatta tatttggtta acaccagtgt atgaatcgcc aatgaatgat aatggttatg
    29041 atataagcaa ttatttagaa ataaatgagg acttcggtac gatggatgat tttgaaaggt
    29101 taattaatgt tgcgcatgaa aaaggtatta aagtgatgtt agatattgta atcaatcata
    29161 catcgacgga gcatgaatgg tttaaagcgg ctcgtaaatc taaagacaat ccatatagag
    29221 attattactt ttttaaagca tctgaagatg ggcctccaac aaattggcat tccaaattcg
    29281 gaggtaatgc atggaagtat gattctgaga cagatgaata ttatttacat ttatttgatg
    29341 tcagtcaagc tgatttaaat tgggataatc cggaagtacg tcaatcgtta tatcgcattg
    29401 tcaatcattg gatagacttc ggcgttgatg gttttcgatt tgatgtcatt aacttaattt
    29461 ctaaaggtga atttaaggac tctgacaaaa taggtaaaga attttatacg gatggtccta
    29521 gagtgcatga gtttctgcat gaattaaatc gtcaaacgtt tggtaacact gacatgatga
    29581 ctgtaggaga aatgtcttcg acgacgattg aaaattgtat taagtataca cagccagaac
    29641 gccaagaatt gaatagtgtt tttaattttc atcatctaaa ggttgattat gttgatggtg
    29701 aaaagtggac aaatgcgaag cttgattttc ataagttaaa ggaaattctg atgcaatggc
    29761 aacgaggtat ttatgacggt ggcggatgga acgcgatttt ctggtgtaat catgatcagc
    29821 cacgggtagt gtctagattt ggtgatgata cgtcggaaga gatgaggata caaagtgcta
    29881 aaatgttagc tatcgcactg catatgttgc aagggacgcc atatatttac caaggtgaag
    29941 aaattggtat gacggaccca cattttacat caatagcaca atatcgtgat gttgaatcga
    30001 ttaatgccta ccatcagttg ttaagtgaag ggcatgctga agcggatgtg ttagcgattt
    30061 taggacagaa gtcacgagat aattcgagaa cgcctatgca atggagtgat gatgttaatg
    30121 ctggatttac agctggtaag ccttggattg atatttcgga aaattatcat caggtcaacg
    30181 ttagacaagc acttcagaat aaagattcta ttttctatac gtatcaaaaa ttaatacaat
    30241 taagacatac gcatgatatt attacgtatg gagacattgt gccacgtttt atggatcatg
    30301 atcatttatt tgtttatgaa cgtcattata agaatcaaca atggctagta attgcgaatt
    30361 tctcagcatc ggctgttgat ttgccagaag gattggcgtg cgaaggtcgt gttgtgattc
    30421 aaactggtac agtggaaaat aatacgataa gcgggtttgg tgcaattgta atcgaaacaa
    30481 acgcgtaaaa taaattgagt ggatgcgttt atatggcgaa acaaaaaaag tttatgaaga
    30541 tttatgaggc gttgaaagaa gatatattaa acgggcagat tcaatatggt gaacaaattc
    30601 cgtctgaaca tgatttggtg caattgtacc agtcatctcg agagaccgtg cgtaaggcat
    30661 tagatttgtt ggcattagac ggcatgattc aaaagattca tggtaaaggg tcacttgtca
    30721 tttatcaaga ggttacagag tttccatttt ctgaacttgt tagttttaaa gaaatgcaag
    30781 aagaaatggg tgtcgcatat ttaactgaag ttgttgtgaa tgaggttgtt gaagcgcatg
    30841 aagtgccaga agttcaacat gctttaaaca tcaattctag tgaatcactc attcatattg
    30901 ttagaactcg tcggcttaac caacatgtga agattgttga tgaagattat tttctaaagt
    30961 cgattgtttc agacataggt aatgatgttg cgagtgattc tatttatgat tatttggaaa
    31021 aggtattaaa tcttaatatt agttattcaa gtaagtctat tacttttgaa ccgtttgatg
    31081 aacaagcata tcaattgttt ggtgatgtat cagtggctta ttcagcaaca gttcgaagta
    31141 ttgtgtattt agaaaataca atgccgtttc aatataatat ttcaaaacat cttgcaaatg
    31201 aatttaaatt taatgacttc tcaagacgtc gtacaaagta aacaatgata taaatgattt
    31261 atacttgcaa ttaactatta aaatatagta atatatatct tgccgtgcta ggtggggagg
    31321 tagcggttcc ctgtactcga aatccgcttt atgcgaggct taaatccttt gttgaggccg
    31381 tatttttgcg aagtctgccc aaagcacgta gtgtttgaag atttcggtcc tatgcaatat
    31441 gaacccatga accatgtcag gtcctgacgg aagcagcatt aagtggatca tcatatgtgc
    31501 cgtagggtag ccgagattta gctaacgact ttggttacgt tcgtgaatta cgttcgatgc
    31561 ttaggtgcac ggttttttat ttttaaaaat attaaaccga ttattaagag ttgaaaatat
    31621 ataattatag aagctacttt cttgaagaca attcagcgta ttatacgtgg aacatgtttg
    31681 tgggaagtag cttttttata tatgaagttt gattcaagtg gaatggatgc gcagtttgaa
    31741 tgatttttta cggatataaa aagtaagaag ttattatttg atgataaaga aatgatggtg
    31801 aaatgagggg gagtacctta caatagaatt attaatgaga tacgttatga ttattgacaa
    31861 tcaaatgcct acggaggaca tatgcaaata tatttaagta ctttaacaga gttagattat
    31921 gataaatctt taaatagtat tgaagaaagt tttgatgata atcctgaaac gagttggcaa
    31981 gcacgtgcga aagtaaaaca tttaagaaaa tctccttgct ataattttga attagaagta
    32041 attgcgaaaa atgaaaataa cgatgtcgtt ggacacgttt tattaattga agtagaaatg
    32101 aatagtgatg ataagacgta ttatggtttg gcgattgctt ctttatcagt caaccctgaa
    32161 ttgcgtggac aaaaattagg tcgtggcttg gttcaagcag tagaagagcg tgcaaaagcg
    32221 caagagtata gtacggttgt tgtagaccat tgttttgact actttgaaaa gttgggttat
    32281 caaaatgctg ctgagcatga cattaaatta gaatctggtg atgcaccgtt acttgtaaaa
    32341 tatttatggg ataatttgac ggatgcacca cacggaatcg taaaatttcc agaacatttt
    32401 tattaattgt tcatttaaga agtaaaggta ttatcatgct ataatgaaag gtaattgttt
    32461 atggaggtgc taacttgaat tatcaagcct tatatcgtat gtacagaccc caaagtttcg
    32521 aggatgtcgt cggacaagaa catgtcacga agacattgcg caatgcgatt tcgaaagaaa
    32581 aacagtcgca tgcatatatt tttagtggtc cgagaggtac ggggaaaacg agtattgcca
    32641 aagtgtttgc taaagcaatc aactgtttaa atagcactga tggagaacct tgtaatgaat
    32701 gtcatatttg taaaggcatt acgcagggga ctaattcaga tgtgatagaa attgatgctg
    32761 ctagtaataa tggcgttgat gaaataagaa atattagaga caaagttaaa tatgcaccaa
    32821 gtgaatcgaa atataaagtt tatattatag atgaggtgca catgctaaca acaggtgctt
    32881 ttaatgccct tttaaagacg ttagaagaac ctccagcaca cgctattttt atattggcaa
    32941 cgacagaacc acataaaatc cctccaacaa tcatttctag ggcacaacgt tttgatttta
    33001 aagcaattag cctagatcaa attgttgaac gtttaaaatt tgtagcagat gcacaacaaa
    33061 ttgaatgtga agatgaagcc ttggcattta tcgctaaagc gtctgaaggg ggtatgcgtg
    33121 atgcattaag tattatggat caggctattg ctttcggcga tggcacattg acattacaag
    33181 atgccctaaa tgttacgggt agcgttcatg atgaagcgtt ggatcacttg tttgatgata
    33241 ttgtacaagg tgacgtacaa gcatctttta aaaaatacca tcagtttata acagaaggta
    33301 aagaagtgaa tcgcctaata aatgatatga tttattttgt cagagatacg attatgaata
    33361 aaacatctga gaaagatact gagtatcgag cactgatgaa cttagaatta gatatgttat
    33421 atcaaatgat tgatcttatt aatgatacat tagtgtcgat tcgttttagt gtgaatcaaa
    33481 acgttcattt tgaagtattg ttagtaaaat tagctgagca gattaagggt caaccacaag
    33541 tgattgcgaa tgtagctgaa ccagcacaaa ttgcttcatc gccaaacaca gatgtattgt
    33601 tgcaacgtat ggaacagtta gagcaagaac taaaaacact aaaagcacaa ggagtgagtg
    33661 ttgctcctac tcaaaaatct tcgaaaaagc ctgcgagagg tatacaaaaa tctaaaaatg
    33721 cattttcaat gcaacaaatt gcaaaagtgc tagataaagc gaataaggca gatatcaaat
    33781 tgttgaaaga tcattggcaa gaagtgattg accatgccaa aaacaatgat aaaaaatcac
    33841 tcgttagttt attgcaaaat tcggaacctg tggcggcaag tgaagatcac gtacttgtga
    33901 aatttgagga agagatccat tgtgaaatcg tcaataaaga cgacgagaaa cgtagtagta
    33961 tagaaagtgt tgtatgtaat atcgttaata aaaacgttaa agttgttggt gtaccatcag
    34021 atcaatggca aagagttcga acggagtatt tacaaaatcg taaaaacgaa ggcgatgata
    34081 tgccaaagca acaagcacaa caaacagata ttgctcaaaa agcaaaagat cttttcggtg
    34141 aagaaactgt acatgtgata gatgaagagt gatacatgac aagcgatata atcgtatgta
    34201 taatgaaaga aacatcattt tattgataaa tatttattga ttttcaagga ggaaatggaa
    34261 tatgcgcggt ggcggaaaca tgcaacaaat gatgaaacaa atgcaaaaaa tgcaaaagaa
    34321 aatggctcaa gaacaagaaa aacttaaaga agagcgtatt gtaggaacag ctggcggtgg
    34381 catggttgca gttactgtaa ctggtcataa agaagttgtc gacgttgaaa tcaaagaaga
    34441 agctgtagac ccagatgata ttgaaatgct acaagactta gtgttagcag ctactaatga
    34501 agcgatgaat aaagctgatg agcttactca agaacgttta ggtaaacata ctcaaggctt
    34561 aaacatccct ggaatgtgat catagatgca ttatccagaa cctatatcaa agcttattaa
    34621 tagctttatg aaattgccag gcattggtcc aaagacagcc caacgtctgg cttttcatac
    34681 cttagatatg aaagaagacg atgttgttca gtttgccaaa gcattagtag atgttaaaag
    34741 agaattaaca tattgtagcg tatgtggtca cattactgaa aatgatccat gttatatttg
    34801 tgaagataag caaagagatc gttcagttat ttgtgttgtg gaagatgaca aagatgtcat
    34861 agctatggaa aaaatgagag aatacaaagg tttatatcac gttttacatg ggtctatttc
    34921 gcctatggat ggaattggac cagaagatat taatattcct tcattgattg aacgcttgaa
    34981 aaacgatgaa gttagcgaat taatcttagc tatgaacccg aacttagagg gggaatctac
    35041 agccatgtat atttctagat tagttaagcc tataggtatc aaagtgacga gattagcaca
    35101 agggttatct gtaggtggcg atttagagta tgctgacgaa gtaacattat ctaaagcaat
    35161 cgcaggtaga acagaaatgt aatgtcttct attaaacatt ttagaattta atactatagt
    35221 aagaaaagtc acagtgtaat cattgtggct ttttttatgg tgtggtgtac tactttattt
    35281 gtggtgtgac ggtggcatgg tttacctagt tttactgagg ggtggggaat ctttaggaag
    35341 caaaccattg gttgtgattt gttatttcta atagtaatga agtgaattgg attatcgaag
    35401 tagatctatg gttatggtgt gttggtggca tggtttacct ggttttactg agggatgggt
    35461 aatcttaaag aagcaaatcg ttggttgtga tttgttactt ctaatagtaa tgattcgaat
    35521 tgaatcatcg aagtaggaat actgttgtgg tgtgttggtg ctattaattt gataaatgcg
    35581 gttgatggtt aatgactagg caaatgaaat tcttttgtaa ttgaaatgat agatgctggc
    35641 ttagtagttg tacttctttg gtctaaagct tattaaatca gcctgtaaag cggtgttttg
    35701 agagattatt taaaacttgt aaatttattt ttaatttctg gtaaaaaaat aaagttctgt
    35761 tttgcggttt tttcgattga tatatttaga gaaaaacctg tttcttgttc taaaaaacgt
    35821 actatctata agtggggggt ttttaagttc gatttttaga atgagagcgt tcagaatgga
    35881 tagtaaaggt gtaattttta ctgttgttaa gcagtttgaa agcctgtata gtatttattt
    35941 gttgaggcaa acaaaacaac tcaacttaag aaataacttg aattactaac gaaaattaat
    36001 tttaaaaagt tgttgactta aatgttaata aaatgtataa ttaattcttg tcggtaagaa
//
LOCUS       DATTQF010000014        35540 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.014, whole genome shotgun sequence.
ACCESSION   DATTQF010000014 DATTQF010000000
VERSION     DATTQF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 35540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.014"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..117
                     /locus_tag="VB241_000565"
     CDS             <1..117
                     /locus_tag="VB241_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HEP1243892.1"
                     /translation="YHLYDDRGLWLAFNNNEDYARYSEKYNDLILEFDNEDI"
     gene            complement(122..338)
                     /locus_tag="VB241_000566"
                     /pseudo
     CDS             complement(122..338)
                     /locus_tag="VB241_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(375..1115)
                     /locus_tag="VB241_000567"
     CDS             complement(375..1115)
                     /locus_tag="VB241_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEP1243893.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYMKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEDADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDNDATLVIHDDMVSVVKSLMTE"
     gene            1321..2112
                     /locus_tag="VB241_000568"
     CDS             1321..2112
                     /locus_tag="VB241_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1243894.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2479..2907
                     /gene="lacA"
                     /locus_tag="VB241_000569"
     CDS             2479..2907
                     /gene="lacA"
                     /locus_tag="VB241_000569"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP1243895.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2923..3438
                     /gene="lacB"
                     /locus_tag="VB241_000570"
     CDS             2923..3438
                     /gene="lacB"
                     /locus_tag="VB241_000570"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP1243896.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3451..4383
                     /gene="lacC"
                     /locus_tag="VB241_000571"
     CDS             3451..4383
                     /gene="lacC"
                     /locus_tag="VB241_000571"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP1243897.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHAGIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPKISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4387..5367
                     /gene="lacD"
                     /locus_tag="VB241_000572"
     CDS             4387..5367
                     /gene="lacD"
                     /locus_tag="VB241_000572"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP1243898.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDASTHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5389..5700
                     /locus_tag="VB241_000573"
     CDS             5389..5700
                     /locus_tag="VB241_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP1243899.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5706..7418
                     /locus_tag="VB241_000574"
     CDS             5706..7418
                     /locus_tag="VB241_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1243900.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7436..8848
                     /gene="lacG"
                     /locus_tag="VB241_000575"
     CDS             7436..8848
                     /gene="lacG"
                     /locus_tag="VB241_000575"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP1243901.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGEYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9114..9983
                     /locus_tag="VB241_000576"
     CDS             9114..9983
                     /locus_tag="VB241_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1243902.1"
                     /translation="MKKIFVISTTLLAVAIIIGTIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLSKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGESAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10266..11267
                     /locus_tag="VB241_000577"
     CDS             10266..11267
                     /locus_tag="VB241_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEP1243903.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEDIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKDGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAESLAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPRAFKNLFTGDNFGKQVIKVTE"
     gene            11502..12509
                     /locus_tag="VB241_000578"
     CDS             11502..12509
                     /locus_tag="VB241_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEP1243904.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPNVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHLLLEKQSMIGKLVIN
                     I"
     gene            12817..14379
                     /locus_tag="VB241_000579"
     CDS             12817..14379
                     /locus_tag="VB241_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEP1243905.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITSFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYASITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLLGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIAEIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDV
                     LEYRSSKHIDEN"
     gene            14554..15090
                     /gene="amaP"
                     /locus_tag="VB241_000580"
     CDS             14554..15090
                     /gene="amaP"
                     /locus_tag="VB241_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP1243906.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRADIKKNVEHFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15103..15342
                     /locus_tag="VB241_000581"
     CDS             15103..15342
                     /locus_tag="VB241_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEP1243907.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTILIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15405..15914
                     /locus_tag="VB241_000582"
     CDS             15405..15914
                     /locus_tag="VB241_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP1243908.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(16075..18051)
                     /gene="sfaD"
                     /locus_tag="VB241_000583"
                     /gene_synonym="sfnaD"
     CDS             complement(16075..18051)
                     /gene="sfaD"
                     /locus_tag="VB241_000583"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HEP1243909.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDNITNMITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAMSYQALSMQHDSTPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVHSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            18153..19346
                     /gene="sfaA"
                     /locus_tag="VB241_000584"
     CDS             18153..19346
                     /gene="sfaA"
                     /locus_tag="VB241_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HEP1243910.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQSICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            19333..21090
                     /gene="sfaB"
                     /locus_tag="VB241_000585"
     CDS             19333..21090
                     /gene="sfaB"
                     /locus_tag="VB241_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HEP1243911.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKESLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIMTLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAIDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            21097..22167
                     /gene="sfaC"
                     /locus_tag="VB241_000586"
     CDS             21097..22167
                     /gene="sfaC"
                     /locus_tag="VB241_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HEP1243912.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISMADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     HINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFIQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(22268..22393)
                     /locus_tag="VB241_000587"
     CDS             complement(22268..22393)
                     /locus_tag="VB241_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243913.1"
                     /translation="MYHGYIIFELIQLTPFSMTVISLIQLILPIAVLCDELLHLI"
     gene            22560..23543
                     /locus_tag="VB241_000588"
     CDS             22560..23543
                     /locus_tag="VB241_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1243914.1"
                     /translation="MRGLKTFSILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIRDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNIDAFKTI
                     AKALDKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDNAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            23555..24586
                     /locus_tag="VB241_000589"
     CDS             23555..24586
                     /locus_tag="VB241_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1243915.1"
                     /translation="MAIKELSSQSAIDHKRKRRTTLTYMVSVCFLFICVYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYSLYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIA
                     TVFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSIAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            24583..25551
                     /locus_tag="VB241_000590"
     CDS             24583..25551
                     /locus_tag="VB241_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEP1243916.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            25787..27157
                     /locus_tag="VB241_000591"
     CDS             25787..27157
                     /locus_tag="VB241_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEP1243917.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLLHNAFNVRIGW
                     LILRLLAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGIVDFFKSGLKTVVDMWFVILPVV
                     MSIGTIATIIANYTPLFVILGKPFVPFLELMHIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(27289..27549)
                     /locus_tag="VB241_000592"
     CDS             complement(27289..27549)
                     /locus_tag="VB241_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243918.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            27726..28241
                     /locus_tag="VB241_000593"
     CDS             27726..28241
                     /locus_tag="VB241_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEP1243919.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMIGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            28285..29472
                     /locus_tag="VB241_000594"
     CDS             28285..29472
                     /locus_tag="VB241_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HEP1243920.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQAKQLKELHRQTGHKIQWYIMTSDINHEETLAYF
                     ESHNYFGYDQESIHFFKQDNIVALSEAGQLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            29491..30174
                     /locus_tag="VB241_000595"
     CDS             29491..30174
                     /locus_tag="VB241_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP1243921.1"
                     /translation="MSKSSEKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAYGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            30352..31695
                     /locus_tag="VB241_000596"
     CDS             30352..31695
                     /locus_tag="VB241_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1243922.1"
                     /translation="MRLKSIVTVIALILIMFMAAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLTRFKIIYIAIAGLTLFTVGSLMSGLSTHFSMLVISRV
                     IQGFGSGVMMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVVWTFHFPEEETVAKSKFDTKGITLFYIFIGLIMFALLNQQH
                     LYLNIIGFVLAILVALRLFNVEKKVSSPFLPVAEFNRMITLVFITDLLTAVCLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHHIEAKLSRKVIYLSSFTLLLLS
                     SIIIAFGIKLPILIACVLILSGLSFGYIYTKDSVIVQEETSPIQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYALKSGLFGANLHNILGVVSLISVGLIVVWLIFYREAASQTKE"
     gene            31817..32290
                     /gene="sepA"
                     /locus_tag="VB241_000597"
     CDS             31817..32290
                     /gene="sepA"
                     /locus_tag="VB241_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HEP1243923.1"
                     /translation="MVMNYLKHKFYNLLTTIIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIVFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYMVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            32479..33922
                     /gene="lmrS"
                     /locus_tag="VB241_000598"
                     /pseudo
     CDS             32479..33922
                     /gene="lmrS"
                     /locus_tag="VB241_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
     gene            34070..35134
                     /locus_tag="VB241_000599"
     CDS             34070..35134
                     /locus_tag="VB241_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEP1243924.1"
                     /translation="MLTVDQVKELIGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     VAMAQLGGAPQLDLQMAVVNVLKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGINGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVRWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            35223..35409
                     /locus_tag="VB241_000600"
                     /pseudo
     CDS             35223..35409
                     /locus_tag="VB241_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12117 a         5015 c         6638 g        11770 t
ORIGIN      
        1 tatcatttat atgatgatag aggtctatgg ttagctttta ataataatga agattatgca
       61 agatattctg aaaaatataa tgatttaata ttggaatttg ataacgaaga tatctgattt
      121 tttaatctaa caaatgacaa accattaaaa gtagcatccc aacatcaaat aaaacacaaa
      181 tcatcaaata aaaatcgcta caaaaccaaa tcgttaagca cgcaataaat aaaatttctc
      241 actcatttta atttggaatt ccaaacgtcg aattccgaaa accaaactcc gaattccaag
      301 ctcaaaaaat gaatacgcaa cttcaaaatt aaaagcattt ccctgttact cgggaaatgc
      361 tttttacgta ctgattactc tgtcattaat gattttacaa cggaaaccat gtcgtcatgt
      421 atgaccaaag tagcgtcgtt atcataaggt gttcgatctt tattgataat aattaaattg
      481 tcgcctttaa aatttgatat taatcctgcg gcaggttgta caacgagtga tgaacctagt
      541 acgacaaggg tgtcagcatc ttcaatttta tttaatgccc gtatgatggt aggttgatct
      601 aacatttcac cgtataatac gatgtccggt cgaatggcac caccacaatt atcacaatgt
      661 ttcaaagttc tatcaataac atctgacttc atataagatt tatgacatgc attacaataa
      721 aaacgattta acgtgccatg taattcatca acatgttgac ttccagcgtc tgagtgcaaa
      781 ccatcgatat tttgcgtgat gacacctaaa gattgttgat tacgttctaa ttttgcaatc
      841 caatcatgaa cgatattggg catcgtatcg acaaatagta agcgcttatg gcagaaattg
      901 ataaaacctt caggatcatc ttctaaataa tcacggctta acaagtattc tggcgaaagc
      961 ccatcttttg aaatttcatc aaataagcca cccattgaac ggaaatctgg aacaccactt
     1021 gcgacagata caccagcacc tgtaaaaaat gtaatacgat tcgaactatc tataatatgt
     1081 tttagtgtct ctaaatcatg tttcaagtta aacaccccat caaaataatg aatgatttat
     1141 aacgtctttt gcttttatta tagtatattc attttacgat tatcaaaagc gcagtttaaa
     1201 cagttctttt taaaaatgaa ttatacatat aatcgaacag ttaaattata ttagttttta
     1261 cgcaattttt attatagaat aagcatgtat attaaaattg atgtctaaac ataaaggata
     1321 atgacccatc gcctaaataa aggaggcaat tttacattga ataaacatga acgtttggac
     1381 gaaattgcta aactagtgaa taaaaagggc acgataagaa cgaatgaaat cgtcgaaggt
     1441 ttaaatgtgt ctgatatgac agttcgaaga gatttaattg aattggaaaa taaagggatt
     1501 ttaacgaaga ttcatggtgg tgcacgcagt aattcaacat ttcagtataa agaaatatcg
     1561 cataaagaaa aacatacccg tcaaattgca gaaaaacgat ttatagctag aaaagctgca
     1621 tcattaattg aagatgggga tactttgttt ttcggaccag gaacaacagt ggaactatta
     1681 gcagaagaag tcaatcatca tacgctcaca attattacga attgtttgcc ggtgtataaa
     1741 attttgttgg aaaaacaaac agcacatttt cgtgtctatt taattggtgg tgaaatgcgc
     1801 catattacag aagcatttgt aggtgaaatg gcgaatgcca tgttagaaaa actaagattt
     1861 agcaagatgt tctttagtag taacgcagta aataaaggtg ctgtgatgac atctacatta
     1921 gatgaagctt atacgcaaca attggcacta agtaattcaa ttgaaaaata cttgctaatt
     1981 gatcatacga aagttgggaa agaagatttt acatcatttt gtcagttaaa cgaattgact
     2041 gcagtggtca tggactatga agatgaagaa aaagtagaaa cgattaaaac atatattgaa
     2101 gtagtcgact aaattaaaaa tataactgca ttgttaaaat atgtgtctat atgctcataa
     2161 gtgaatgtga atttgcattc atttatgggc tatttttttg caaatttatg taaacgggaa
     2221 ccccgacaaa tttttactac aatttcagac gaatacctaa ttaaaacaac accacaataa
     2281 gaatgttcac ttattgtttg aaaatatgtc ataaacaaac agattataaa catattatgt
     2341 tgatttattg tttgtttatg tttataattt aaacgtaatc aaattgaaag ccttttctca
     2401 agattttcat aacctacaaa taaatatgtc atattttaag aaaggtattt caaaattaaa
     2461 gtaaaaagga gtcttattat ggcgattatt attggttcag atgaagctgg caaacgatta
     2521 aaagaagtca tcaaatcata cttattagac aataaatatg acgttgttga cgtaacagaa
     2581 ggacaggaag ttgactttgt tgatgcaact ttggctgtag caaaagatgt tcaaagtcaa
     2641 gaaggtaact taggtattgt tatcgatgca tttggtgcag gtagctttat ggttgctaca
     2701 aaaatcaaag gcatgattgc tgctgaagta tcagatgaac gctctggata tatgactaga
     2761 ggccataaca attcaagaat gatcactatg ggatctgaaa ttgttggaga cacattagcg
     2821 aaaaatgtcg ttaaaggttt tgttgaaggt aaatacgatg gtggtagaca ccaaatccgc
     2881 gtagatatgc ttaataaaat gtgctaagga ggaaaaatta ctatgaagat tgcattagga
     2941 tgcgaccata ttgttacaga tacaaaaatg cgtgtatctg aatttttaaa atcaaaagga
     3001 catgaagtca ttgacgtagg aacatacgat ttcacaagaa cacattatcc aatttttggt
     3061 aaaaaagttg gagaacaagt tgttagcggt aatgcagact taggtgtttg catttgtgga
     3121 acaggtgttg gtattaacaa tgctgtaaat aaagtacctg gcgttcgttc agcactagta
     3181 cgtgatatga catcagcgct atatgctaaa gaagaattaa atgcgaacgt tattggcttt
     3241 ggtggacgta ttattggtga gttattaatg tgcgatatta tcgatgcatt tattaatgct
     3301 gaatataaac caactgaaga gaacaaaaaa ttaatcgcta aaattaaaca tttagaaaca
     3361 agcaatgcag atcaagctga tccacatttc tttgatgaat tcttagaaaa atgggacaga
     3421 ggcgaatacc acgattaagg gacgacacat atgattttaa ctttaacatt aaatccatcc
     3481 gttgatattt catacccatt aacagcattg aaattagatg atgttaatcg tgttcaagaa
     3541 gtaagtaaaa cagctggtgg taaaggttta aatgtaacaa gagtacttgc gcaagtaggt
     3601 gagccagtcc ttgcgagtgg ttttattggc ggtgaattag gtcaatttat tgctaaaaaa
     3661 ttagatcatg ccggcatcaa gcatgcattt tataacatta agggtgaaac acgaaactgc
     3721 attgcaattt tacatgaagg acaacaaaca gaaattttag agcagggacc ggagattgat
     3781 aatcaagagg ctgcaggatt tattaaacat tttgagcaat tactagaaaa agttgaagca
     3841 gttgctattt caggtagctt accaaaagga ttaaatcaag attattatgc acaaatcatt
     3901 gaacgatgcc aaaacaaagg tgtgcctgtg atattagact gctcgggtgc aactttacaa
     3961 acagtattgg aaaatccata caaaccaaca gtcattaaac caaacatttc tgaattatat
     4021 cagttgctta accagccact tgatgaaagt ttagaaagtt tgaaacaagc agtttcacaa
     4081 cccttattcg aaggaattga gtggattatt gtgtctttag gtgcacaagg tgcgttcgct
     4141 aagcacaatc acacattcta tagagtgaat attccgaaaa taagcgtatt aaatccggtt
     4201 ggttcaggtg attctacagt tgctggaatt acttcagcaa ttcttaatca tgaaaacgac
     4261 cacgatttac taaaaaaagc caatacttta ggcatgctga atgcgcaaga agcacaaaca
     4321 ggttatgtca atttaaataa ttatgatgat ttattcaatc aaattgaagt tttagaggtg
     4381 taaaaaatgt caaaatcgaa tcaaaaaatc gcatcaattg aacaattaag taataacgaa
     4441 ggtatcattt ctgcattagc atttgaccaa cgtggtgctt taaaacgcat gatggcaaaa
     4501 catcaaactg aagaaccaac tgtagctcaa attgaacaat taaaagtatt agttgcggaa
     4561 gaattaacgc aatatgcatc ttcaatttta ttagatcctg aatacggttt accagcttct
     4621 gatgcacgta acaaagattg tggattatta cttgcttatg aaaaaactgg atatgacgta
     4681 aacgctaaag gtcgtttacc tgactgttta gtagagtggt ctgcaaaacg tttaaaagaa
     4741 caaggtgcaa atgctgttaa attcttactt tactatgatg ttgatgatgc tgaagaaatt
     4801 aacattcaaa agaaagcata tattgaaaga atcggttcag aatgtgttgc agaagatatt
     4861 ccattctttt tagaagtatt aacatacgat gacaacattc ctgataacgg tagtgttgaa
     4921 tttgcaaaag ttaagcctag aaaggttaac gaagcaatga aattgttctc agaaccacgc
     4981 tttaacgttg atgtattgaa agttgaagta ccagtaaaca tgaagtatgt agaaggcttt
     5041 gctgaaggtg aagtggttta tacaaaagag gaagcggcac aacactttaa agatcaagat
     5101 gcatcaactc atttaccata catttactta agtgctggtg tttctgcgga attattccaa
     5161 gaaactttaa aatttgctca cgaagctggt gctaaattca acggtgtact ttgtggccgt
     5221 gctacatggt ctggtgctgt tcaagtatac attgaacaag gtgaagatgc tgctcgtgaa
     5281 tggttacgta caactggttt caaaaatatt gatgacttaa ataaagtatt aaaagatact
     5341 gcgacatctt ggaaacaaag aaaataagta taaaagggga ttaatgctat gaatagagaa
     5401 gaagtccaat tattaggttt tgaaattgtt gcatttgcag gggatgcacg ttctaagttt
     5461 ttagaagcat taacagcagc tcaagctgga gattttgcaa aagcagatgc actgattgaa
     5521 gaaggaaaca attgcattgc tgaagcgcat agagcacaaa caagcttatt agctaaagaa
     5581 gcgcaaggtg atgatattgc atacagtgtg acgatgatgc atggtcaaga ccatttaatg
     5641 acaacaattt tactgaaaga tttaatgaag catttattag aattttataa aagagggtga
     5701 tgacaatgat gcaaaaattg atcgcacaaa ttgaaaaagg gaagccattt ttcgagaaat
     5761 tatcacgtaa catatattta agagccatcc gtgatggatt tatttctgca atgcctgtca
     5821 tcttattctc aagtatcttt ctattaattg catacgtgcc aaatatattt ggttttaaat
     5881 gggataaagg catggaagcc attttaatga aaccatataa ctatacgatg gggcttgtag
     5941 catttttagt agcaggaacg acagcgaaat ctttaacaga ttcatttaac cgtaagctag
     6001 aaagtacaaa tcaaattaac tttatttcta caatgctagc agcgatgtgt ggattcttgt
     6061 tcttagcttc tgatccagcc aaagatggtg gattcttaag tgcgtttatg ggaactaaag
     6121 gtttattaac agcattctta tcagcatttg taactgttat tgtttataac ttctgtgtga
     6181 agcgcaatat tacaattaaa atgccgaaag aagtaccgcc gaatatttca caagtattta
     6241 aggacttaat tccattttca gcggtaatca tcattcttta tgcactagat ttagtcattc
     6301 gcaacagctt taaatcaaat gtagcggaag gtattttaaa attattcgaa ccattattta
     6361 cagcagcaga tggatggata ggtgtcacaa ttatctttgg tgcctttgca ttattctggt
     6421 ttgtaggaat tcatggtcca tcaatagtag aaccagcaat tgcagccatt acatatgcga
     6481 atatcgaagc gaacttcaag ttgcttcaag ctggtgaaca cgcagataaa attattacat
     6541 caggtacaca aatgtttatc gttacatttg gtggtacggg tgcaacattg gtcgtgccat
     6601 ttatgtttat gtggatgacg aaatctaaac gaaacaaagc gattggacgt gcatctgttg
     6661 taccaacatt ctttggtgta aacgagccga ttttattcgg ggcaccacta gtattgaatc
     6721 ctgtgttctt tattccattt gtattagcgc caattgtgaa cgtatggata tttaaacttt
     6781 tcgttgaagt gttaggaatg aacagtttta gtgtgaactt accatggaca acaccaggtc
     6841 cactcggcat catcatgggt actggatttg gtttatggtc attcgtacta gcgattactt
     6901 taattgttgt agatattatt atttactacc cattcctaaa agtttatgat agtgaaattc
     6961 ttgatgaaga agaaggacgt aaagaaagta attcagattt aaaagaaaaa gttgcagcaa
     7021 actttgatac gaaaaaagct gattcaattt tagcggcaag tggtgtatca gacgatgcag
     7081 ctaaagcttc aaatatcaca gaacaaacaa atgtcttagt actttgtgcc ggtggtggta
     7141 caagtggatt attagccaac gcacttaata aagcagctga agaatatcat gtgccagtga
     7201 aagcggcagc tggcggatat ggcgcacata tggacattat gaaagagtac caattaatca
     7261 ttttagcgcc acaagttgcg tctaactatg aagatattaa gcaagataca gatagattag
     7321 gtattaaatt agcgaagact caaggtgctg aatatatcaa attgacacga gatggccaag
     7381 cagcccttga tttcgttcaa caacaatttg aaaattaaga ataggagttt ttcatatgac
     7441 taaaacatta cctgaagatt ttatttttgg tggagcaaca gcagcatatc aagcagaagg
     7501 tgcgacaaat acggatggta aaggtcgcgt agcatgggat acgtatttag aagaaaacta
     7561 ttggtacact gcagaaccag cgagtgattt ttataacaga tatccagtgg atttagaatt
     7621 aagtgaaaaa ttcggtgtta atggtatccg tatttcaatt gcatggtctc gtatcttccc
     7681 aaatggatat ggcgaagtta accctaaagg tgttgagtat taccataagt tatttgcgga
     7741 gtgtcataaa cgtcatgttg aaccatttgt aacattgcat cattttgata caccagaagt
     7801 gttacacaaa gatggcgatt ttctaaatcg taaaacgatt gactactttg tagattatgc
     7861 tgaatattgt tttaaagaat tcccagaagt aaaatattgg actacattca atgaaattgg
     7921 accaattggt gatggccaat atttagtagg taaattccct ccaggtatta aatatgattt
     7981 tgaaaaagtt ttccaatctc atcataatat gatggttgct catgcaagag cagtaaaatt
     8041 atttaaagat ggcggctatc aaggagaaat tggtgttgta cacgcattgc caacaaaata
     8101 tccgttcgat ccatcaaatc ctgaagatgt tcgtgcagca gaattagaag atatcatcca
     8161 taacaaattc atcttagatg caacatattt aggtgagtat tctcgtgaaa cgatggaagg
     8221 tgtgcaacat atcttatctg ttaatggcgg taaattaaac attacagatg aagattatgc
     8281 cattttagat gcagccaaag atttaaacga cttcttaggt atcaattact acatgagtga
     8341 ttggatgaga ggttatgatg gagaatctga aattacgcat aatgcgacag gtgataaagg
     8401 cggttctaaa taccaactta aaggtgtcgg tcaacgtgaa tttgatgtcg acgtacctcg
     8461 cacagactgg gactggatga tctatcctca aggtttatat gatcaaatca tgcgtgttgt
     8521 taaagattat cctaactatc ataagattta tatcactgaa aatggtttag gatataaaga
     8581 tgaatttatt gaatctgaaa aaacagttca tgatgatgca cgtattgatt atgtaagaca
     8641 acatttaaac gttattgcag atgctattaa agatggtgca aatgttaaag gttactttat
     8701 ttggtcatta atggatgtgt tctcttggtc aaatggttat gaaaaacgat atggtttatt
     8761 ctatgtcgat tttgagactc aagaacgcta tcctaagaaa agtgcttatt ggtacaaaga
     8821 gttagccgaa acaaaagaaa ttaaataata aaagatatta aaaggcctat cagtcgtgtg
     8881 ctggtaggtc tttatttata aaaagagaaa aattaaacat gcatatacat atttttatat
     8941 taatcaatgg gagacacacg attgaaagtt aaataagcaa tcaattaaca aacgttaacg
     9001 gacaattttc caaagtcatc tttaaagtta atgatgtatt caaaatcaac taaaggacat
     9061 ttttaatcac atcctttata cttaaataaa agacatgaat gggagttaaa agtgtgaaga
     9121 aaatttttgt aattagtacg acattgttag cggtcgctat tattataggc actattatta
     9181 cggttgtgtt tagtcaacga caggcacaaa catttaaaat acaacaacag caatttgtta
     9241 aaaagccaat tccgacattg tttttacatg gatttggtgg tagtgccaac tctgaaaaat
     9301 ttatggtgaa gcaagcagaa aaaagagggg taactaaaga tatcatcact gcttacgttt
     9361 caaaggatgg tgcagtgaca tttaaaggga aactaagtaa agatgcagtg aatcctattg
     9421 tgaaaattga acttgaaaat aacagacaag gctatttgga taagaatgct gcgtggttta
     9481 aaaatgtact gactaagtta caatctgaat acaatttcga taaattcaac tttgtcggtc
     9541 actcaatggg gaacttaaca tttgcacaat atatgatgac atatggtaac gacaagtcac
     9601 tgccacagtt aaacaaacaa gtgaatattg ctgggacatt taatggcgtt ttaaatatga
     9661 atgaagatgt caatgaaatt acagttgata aagatggtaa accaagtcga atgaatcaac
     9721 cttatcagca attacgcgtg ctaaaagata tttataaagg taaaggtatc gaagtgctaa
     9781 atatttatgg agatttgaaa gatggtacac attcagacgg cagagtgtct aatagttctt
     9841 cgaaatcatt aaaatattta ctgggaaata gtccgaaaag ttatcgagaa tctaaatatg
     9901 aaggtgagtc tgcacagcat agtcagcttc atgagaatga aaatgttgct aatgagttga
     9961 tcgacttctt atggaaaaaa taaagcaact gctagttttt cgggggcaaa aaagcttgta
    10021 atattagagt ttttcaaaaa ttatctattg tttgagagtt tccataaaat gtctaagtcg
    10081 taacatttta aatttatgat tatagcaaat ataatgtggt tgaagttaca tgaatgaatc
    10141 agtgcttaag tccaaatata acaactaaga atgtatttgc atacagacat cacgaagaca
    10201 caattcaaac ttaacgctta agtaacaagt aagtaaaagt ttcaaataaa gaaaggtgat
    10261 taatgatgca aaataaacaa attcttttta acaaaatacc agaaggcatg cctcaagagg
    10321 atacattcaa aattgaagac attgacacgc ctaaacttga gagcgatggt gtacttgtac
    10381 aaacgctgta tatttcagtg gatccatata tgagaggacg tatgactaag gcagattcgt
    10441 atgtacagcc ttttgaaatt ggtaaaccga ttgttagcca tgtcgttgca aaagtaattg
    10501 attcgacact cgcagattat aaaaaaggag atgtcgttgt aggtatgttg ccttggcgaa
    10561 tcattaatca tgtccaagcg gatcaaatta ctaaagttcc aacgacagat gtaccacttg
    10621 atttatattt aagtgtctta ggaatgccag gacaaacggc atatcatggt ctgcttgata
    10681 ttggacaacc taaagacggt gatactgttg tggtttcagc tgcatctggc gctgtaggtt
    10741 cagttgtagg tcaaattgct aagattaaag gttgccgtgt tgtaggtatt gcgggtggcg
    10801 acaaaaaagt aaattattta acagagacac tgggctttga cgctggtatt gattataaga
    10861 aagaggattt tgcagaatcg ttagcacaag cagtaccaga tggtattgat gtttattttg
    10921 aaaatgttgg tggtacagtt ggcgatgaag tatttaagca tttaaatcgt tttgcacgtg
    10981 taccagtatg tggcgcaatt tcgtcatata atcatcctga agcagatatc ggaccacgta
    11041 ttcaaggtac gcttattaag aagcaagtaa tgatgagagg cttcttagta agtgaatttg
    11101 ctaatgcttt taaagaagct agtgaacagc tcgcaacatg ggttcaagaa ggtaaaattc
    11161 agtcacaagt aaccattgaa gatggttttg aaaatgcgcc acgtgcattt aaaaatttat
    11221 ttacgggcga taactttggt aaacaagtta ttaaagttac ggaataataa agtatacatt
    11281 tttacaaatc aactttgtta attgaccaaa atttaataac tttcttatct aaagcaaatt
    11341 tgttcagtaa catggtagaa ataagtctaa aatgtaatac gaaagtcgtc tgtaattatt
    11401 tatatattca aatgaaaaag gagtcatgat gctgcgacat aagcacacat ggcttctatt
    11461 ttgtataata agaaataagt tcataaatga ggtgaaacga tatgaaaatg ataggatttg
    11521 aaaagccctt taaattggaa gaaggaaatt tatttaaagt atacgaacaa agaaagccaa
    11581 cgccagaaaa cgatgatatc ttagtcaaag taaatagcat tagtgtaaat cctgtagata
    11641 caaaacaacg acaaatggaa gtcactcaag cacctcgtgt acttgggttt gatgctattg
    11701 gtactgttga agctatcgga ccaaacgtta cattatttag tccgggtgac gttgtatttt
    11761 atgctggatc gccaaataga caaggatcaa atgcgacata tcaattggtt tcagaagcta
    11821 tagttgcaaa agcgccacac aacataagcg ctaatgaagc ggttagtcta ccgctaactg
    11881 gtattacggc atatgaaacg ttttttgata catttaaaat ttcacataat ccttctgaaa
    11941 atataggtaa atccgtgtta atcattaatg gcgctggggg tgtaggaagt attgccactc
    12001 aaattgctaa acggtatggt ttaactgtca ttacaacagc atcacgacaa gaaacaacag
    12061 agtggtgtga aaagatgggc gcagatatcg tattgaatca taaagaggat ttagtgcgtc
    12121 aatttaaaga aaaagaaatt ccattagtag attatatatt ttgcacctat aatacagact
    12181 tgtattacaa tactatgatt gaactgataa aaccattagg gcatattaca actatcgtag
    12241 cttttaatga agatcaagat ttaaatgcat taaaattaaa aagtataaca tttacacatg
    12301 aatttatgtt tgcgcgtcca attcatcgaa cgcctgatat gattaaacaa catgagtatt
    12361 tagaagatat tacgaaaaat atagaatcag gacattacca accaacaacg acacaagttt
    12421 ttgaaggttt atcacctgaa aacttatatc aagcacatct attgttggaa aaacagtcaa
    12481 tgattgggaa attagtaatt aatatataga ttagtaagta aagtcgcctt atgcagaaaa
    12541 tactgtatag ggcgacttat tttttgtgtt tagcgactat tgattctaaa gtgaaatatg
    12601 gcaactttgc aggtatcaat tcaataatgc attagaaagg cattacttaa atacaataaa
    12661 atactttgtt gttcaatgat aaataatttg agagtatttt atatatcatt tttaaaagat
    12721 gaagcattat taattgtgtc tatataaaat tacaaatgat taaaatgttg cagttgtggg
    12781 tatgaactta ccattacaca gaaagggttt gtatttatga aaaataaatc agtaagtcta
    12841 gtgttttggg tatcgttagt gatttgtacg atatttgttg cttttggtgc aatatttccg
    12901 aaacagttag aaaaactcac gcaaaacatt acttcattta ttgcgctaca tttttcttgg
    12961 tattatttat tgctcgtttt agttatttta tttgtctgtg tgtatatatt attttcaaga
    13021 tacgcatcaa ttacattagg tgaagaaggt gaagacccag aattttcatt gccatcttgg
    13081 tttgctatgt tatttagtgc tggtatgggt ataggacttg tgttctggac aacggcagaa
    13141 ccaatcagtc acgccttcaa attaacgcct attcataaag ctggcacgca atccgctatt
    13201 aatgatgcaa tgcagttttc atttttccat tgggggattc atgcatgggc agtctatggt
    13261 attgtcgcgc ttgtgtttgc atattttagc tttcataaag gatatcctgg cttagtcagc
    13321 gctacattga cgcctttatt aggtgaaaaa gcaatgcgtg gccctttagg tggtgccatt
    13381 gatgtattgg cagtcatagc tacagtaaca ggcgttgctg caacattagg tttcggtgca
    13441 ttgcaaataa acgaaggttt acatttttta tttaatgtac cgagtaactt cacgatgcaa
    13501 gtaatcttga ttgtcattgc aactatatta tttacttggt cagcatggtc aggtatcgat
    13561 aaaggtatca aaacgttgag taatatcaac atgttacttg cttttgtagt acttcttggt
    13621 ctttttattg ttgggccaac gctttacatt ttaaatacat ttacgaatgg tctaggtaat
    13681 tatattgcaa atttctttag tatgagttta cgcattccgt ctggcggtca aaaattccaa
    13741 tggttacaaa attggacaat cttttactgg gcatggtgga tatcttgggc accatttgta
    13801 ggtatattta ttgcacgtgt gtcaaaagga cgtacgatta aagaatttat tctaggtgta
    13861 ctttttgtcc cggcgttagt ttgctttata ttctttgcgg tattcggcgc gtcagcaatt
    13921 tacttacaag ataatcatat tgccgaaatt gctaaagctg cgacagaaac agcaactttt
    13981 gcaacgctac aacattaccc actaggtttt gtattaagct taattacttt acttgttatt
    14041 atgattttct ttgttacatc agcggactcg gcaacttatg ttttagggat gttgagttca
    14101 agtggtgata ttaatccaaa gtcatttgtc aaagtgagtt ggggtatcat tatggcatta
    14161 tttgcaatta ttatgattta cacaggtggt acgcaagcga tacaaaattt attaatcatc
    14221 gcagcgttgc cattttccgt ggtcattatt gcgatgatat ggtcgctttt aaaatcttta
    14281 agtgaagaaa aacctagaaa tagtaataag gttttaatta aacatcgtga tccagacgta
    14341 ttagaatatc gttcttcaaa acatattgac gaaaattaat taacaaagag gttttattta
    14401 caaaaactag ggaatatatt actaaatggt acttcatctt atatattaaa aataaattta
    14461 aagttattaa tttattatgt ttaagaaaaa atctgatggg tacatattta atatgttttt
    14521 aaattaactt taactaagga ggtgtcgtta ggggtgaaac gacttaaaaa ctttatcctt
    14581 ggcttattaa tagtagtaat cgtggggttc ctgttattca tgtatatcca agatggacgt
    14641 ataactgaat atcaagacta cttccttcaa tttgaatggt tccaaccatt acttatttca
    14701 cttgctgcac tattgatatt aataggtctt atattagtat ttagtatctt caaacctacg
    14761 catcgaaaac ctggattata caaagacttc gatgatggtc atgtttacgt atctcgtaaa
    14821 gctgttgaaa aaactgcatt tgatacagtt gcaaaatatg accaagtaag acaaccgaat
    14881 gtagttgcaa aactatataa caaaaaaaat aaatcttata tcgacattaa aactgacttt
    14941 ttcgtaccga ataatgttca ggtacaatct ttaactgaag caattcgtgc ggatattaaa
    15001 aagaatgtag aacattttac agaaatgcca gtacgtaagt tagaagtaaa tgtgagagat
    15061 cagaaaactt ctggtccacg agtgttgtaa gggaggtata tcatggctaa caatcataac
    15121 caaaacggac aagactctac acaacaagtg attaattttt taaaagtatt taaatggaga
    15181 atcgttggtt tcttagcgtt tctattaatc gcgatattat tcttaacgtt aggtttttgg
    15241 aaaacaatac ttatcatcgt tttatgttta attggtgtag gtattgggta tatgaaagac
    15301 cgtaagcaag attttatgaa ttttttaaat cgatggagtt aattcatcaa tttagaattt
    15361 aattaaaaaa atataaaaaa taacaagtaa aaggagaatc tattatgact gtagataaca
    15421 ataaagcaaa acaagcatac gacaatcaaa ctggtgttaa cgaaaaagaa agagaagagc
    15481 gtcaaaaaca acaagaacaa aatcaagagc ctcaattcaa aaacaaatta actttctctg
    15541 atgaagttgt tgaaaaaatt gctggtatcg ctgcacgtga agttaaaggt atcttagaca
    15601 tgaaaggtgg cttaactgat acattcacta atgcattctc aagtggcaac aacgttacac
    15661 aaggtgtatc tgttgaagtt ggtgaaaaac aagctgctgt agacttaaaa gtaattttag
    15721 aatatggtga atcagcacct aaaatcttcc gtaaagtaac tgaattagtt aaagaacaag
    15781 ttaaatatat tactggttta gatgtagttg aagttaacat gcaagttgac gatgtaatga
    15841 ctcaaaaaga gtggaaacaa aaacacgaaa aaaataatga aaacaataac caagaaagac
    15901 aaggtttaca ataagttatt cgataaaaaa gctcggcaca ccattttagt gccgagcttt
    15961 tttgtgattt ttaataattt gaatatggaa aatgtattat tctcttattt gtatagattg
    16021 tatttaataa gttaatgtaa tccttgagct cacgattaat gaaattctat aattttaatt
    16081 attttctcga tacaaagggt tgttaacttt aatataagta taatgatgtg cttcatcttc
    16141 aagccgcatt gttgtaacac acttataatc tataaatggc gcgaacatgg tatctttaat
    16201 ttcatttata cgattctcat ttacttgatt agatttgtgt gtcgaatgta caagttgatc
    16261 aaagatgtta tctagtacat cacgaacgat ataccacata tgtctttcta agtttgaatc
    16321 attcgatgct ttagagattg taagaattaa ttcgcctaaa tggttttgaa cggtagaata
    16381 aaaggctttg ttaaacacag acgtttttga atcagtaaga attcttgatt tttcatggaa
    16441 atgagatgta ctgtatccca tttcatttag ttgtgcttta tcaattctta aaccttcaaa
    16501 gtctctaata tacatcgtgt ccaatagacc atctttccga aatgttgcaa tagcattttg
    16561 caagtgagcc tctaatgcaa tgccatattt agtaactagt gggattacga gaccaagcaa
    16621 tgctttactg taagtttcaa tccacgattt cgccgatgat tcaaaatcag acaatgatgc
    16681 agctgattga taacgtttaa tcaatgtcac gataggtgat tcattgttaa atgggtaggt
    16741 tgcaactaag cttgaaggaa tcattggtgt gacttcttgt ggaatcattt ggtatatgtt
    16801 ttttctaaat aatgtaccta attgttcact tcgttcagtt tgatagtctg cttcgtcttg
    16861 ctcattataa aaatgaatac cagcaacttc atcaataatt gttgatgcat aggacttaaa
    16921 tatgacatct ttctccaaaa tatcatttaa aatacgtgtc attagtggac cattgtgcgt
    16981 cgtttgttct gataatgtac gaatttcacc tgtaatatga acgtttgtcg acaatttgat
    17041 gtgtggcgac atagctgggt atttaggaac taatgttctg aaagataaac cggcataata
    17101 atccaacgta tgttttgctt caatgattaa ttctttatct acttctgctt gataatcaga
    17161 atgtaatacg tcatctaatt gccatggatg tacaatcata atgtgataat cattaaagtt
    17221 aaagtttggt gtaaattcat tttccaattg tttaattaag tccggaaata gttgatgaac
    17281 agttgtatca taatctttag acagtgacat agtacggctt aatttactgt gaatcagtac
    17341 tattttcaac ttaataggct gattgaattc tgaagaatat aagaatgttt gtaatgcatt
    17401 taaaccttta cgtaatttag ccccgggatg tagcggatga ccttcaataa cggcttgctc
    17461 tgaacgcaag taactatctt cgctattttc gataatatta aataaaggtg tagaatcatg
    17521 ttgcattgac agtgcttgat agctcatcgc aaatgtcata ttagttgcac tgtttattaa
    17581 atcttgctga aattgatcac tagcagcatt ttttaaatct ggtgcttcaa ttaaaataca
    17641 ctcaagaatt tcatttggat ggtgtactcg tgtaatcata tttgtaatgt tatctttaat
    17701 gtagaaaggg ccttcaacat caattcgatc aaaggcgtgt tctccagtga taggagcata
    17761 taatgtttgc ttagcttgtg ggaactggat ttctagtata tgagtcgttg agatatctaa
    17821 cataatcaaa tcacgactca atattttctt actttgagtg cgcgctttaa ctaagttttc
    17881 gcgatgcatt gatgtgacta atctctgagt gactttatct cttccttgta aaatcatctc
    17941 tttaaaaata ttagcccaat cgctattatg ttgttgtaaa aataaatatg tttcttgttc
    18001 ttctttgtta aattttaatg tctgttcttt aaaaattaag ttcaagttca taattcacct
    18061 ctatgaaata ttttacaaaa gcaagataga tttgtataat ccatattaat gataatgatt
    18121 cttattatca acagaatgcg ggtgtaagtt ttatgacaaa atattttttt agcagttctt
    18181 ttctactatt tctaggtaat tggattggac aaatagggct aaattggttt gtacttacca
    18241 cttatcataa cgcagtttat ctggggattg tcaatttttg cagacttgta ccaatattat
    18301 tactaagtgt gtgggcaggg gcaattgccg ataaatatga taaagggcga ttgctgagaa
    18361 ttacaatttc atcatcattt ttagtaactg caattttatg tgtgcttacg tatagtttca
    18421 ctgcaattcc aattagcgtc attattatat atgcgacatt aagagggatt ttaagtgcag
    18481 ttgaaacacc tttaagacaa gcaatcttac cagatttatc agataaaata tctactacac
    18541 aagctgtatc atttcattca ttcatcatta atatttgtcg ttcaataggg cctgccattg
    18601 ctggtgtcat attagcagtc tatcatgcgc caacaacatt tcttgcacag tcaatttgtt
    18661 attttatcgc agttttatta tgcttaccat tacattttaa agtaactaaa atacctgaag
    18721 atgcaacaag gtacatgccg ttaaaagtca ttatagatta cttcaaatta catatggaag
    18781 gtcgacaaat atttataaca tcattattga ttatggcgac aggtttttca tatacgacac
    18841 ttttaccagt tttaacaaac aaagtatttc cggggaaatc tgaaatattt ggtatcgcta
    18901 tgacgatgtg tgccattggt ggtattattg caacgctagt tttacctaga gtacttaaat
    18961 atattggtat ggtaaatatg tattatttaa gttcattttt atttggtatt gctttgttag
    19021 gtgtggtatt tcacaatatt gtcatcatgt tcatttgtat tacattgatt gggttattta
    19081 gtcaatgggc acgtacgaca aatcgcgttt attttcaaaa taatgttaaa gattatgaac
    19141 gtggtaaagt actgagtatt attatgatgg atagaggtat gattccattg ggaagtctgt
    19201 tgatgagtat atgtgcagat gtgtttggca ttgttagaac tttttcaata atgggaataa
    19261 gtactatatg cattacaatg gtattctatt ttataaatag aaagttgaag ttaaagttgg
    19321 aggaaagtaa tcatggtata tcttgaatgg gcaaaggcag atagaaatat tcaatatcgt
    19381 gtaattaacg ccattattaa agaacgtatt taccccgagc aaacatttat ttcacaaaaa
    19441 gaatctttaa ttgaaattca gtatcatatg catgtgttga ctattgaagt tgttagaaaa
    19501 agtgcattag aacgctatga gtttacaggt gatattactt atttaaataa aggtgaaacg
    19561 tcgctaatca tgacgttaga agggctacta gatgtgttga atcatgactt tgatatccct
    19621 atttcagagc gactacgcga agagttaata cacagtcgag atagtttagt tgaaacatat
    19681 aagcaaatgt ctcacagaca aacattaata agtcaaagtt ttaaattttc aagattacca
    19741 caagatatta actttttttc atggttacaa catgtaaaag atagtgataa gacagatgat
    19801 ttaacttatt ctgagagttt ggtaccagag gggcatccaa cacacccttt aaccaaaacg
    19861 aaattgccct taactatgga agaagtacga gcatatgcac ctgagtttga aaaagaaatc
    19921 cctttgcaaa ttatgatgat tgaaaaagac catgttgtgt gcacagctat agatggtaat
    19981 gatcaattta ttattgatga aataattccc gaatactaca atcagattcg tgtgtttttg
    20041 aagagtttag gtttgaaaag tgaagactat agagcgattt tagtacatcc ttggcaatat
    20101 gatcatacga tagggaaata ttttgaagca tggattgcta aaaaaatatt aattccaacg
    20161 ccgtttacaa tactttcaaa agcaacttta tcattcagga cgatgtcttt aattgataaa
    20221 ccatatcatg ttaagttgcc cgtcgatgca caagcaacaa gtgccgttag aacagtctca
    20281 actgtgacta ctgtagatgg accaaagtta agttatgctt tacaaaacat gttgaatcaa
    20341 tatccaggat ttaaagttgc tatggaaccg ttcggtgaat atgcaaatgt tgataaagat
    20401 agggcgcgtc agcttgcttg tattataaga caaaagcctg aaattgatgg aaaaggtgca
    20461 acagtagtta gtgcaagtct agttaataaa aatccaatag atcaaaaagt tatcgtggat
    20521 agttacttag agtggttaaa tcaaggaatt actaaagaaa gtattacgac atttattgaa
    20581 cgatactctc aagcattaat cccgccttta attgctttta ttcaaaatta tggaattgca
    20641 ttagaagcac acatgcaaaa tacagtagtg aacttggggc cacattttga cattcaattt
    20701 ttagtgagag atttaggtgg ttctagaatt gatttagaaa cattacaaca tcgtgtatca
    20761 gatattaaaa ttacaaatga tagtttaata gctgattcta tagatgcagt gattgcaaaa
    20821 ttccaacatg ctgttattca aaatcaaatg gcagaattaa tccatcattt taatcagtat
    20881 gattgtgttg aagaagccga attatttaac atagtacagc aagtagtagc gcatgccatt
    20941 aacccaacat taccacatgc aaatgagtta aaagatattt tgtttggacc gacaattact
    21001 gtcaaagcgt tgttaaatat gagaatggaa aataaagtaa agcaatattt aaatattgag
    21061 ttagataatc cgataaaaaa agaggtgtag tactacatgg cacacgttaa tataaatata
    21121 tcgaagatta aatataacgc caaagtactt caaacagttt ttcaaagtaa aaatattcaa
    21181 ttcacaccag taattaagtg catagctggt gaccgtacaa ttgtagaaag cttaaaagcg
    21241 ttaggtatca atcatgttgc agaatccaga ttggataaca taattagtat ggcagatcag
    21301 gatttaacat atacgttatt gcgtacgcct gccaaaaaag agatttcaga tatgatagaa
    21361 aaagttgata tgagtatcca aacagaatta tctaccatac atcatataaa tgaagtagca
    21421 gaagttttag gaaaaaaaca taagatttta ttaatggtag attggaaaga tggccgtgaa
    21481 ggtgttctga cttatgatgt tttagattat attaaagaga ttattcactt gaaaaatatc
    21541 cattttgttg gtttagcatt caattttatg tgttttaaat ctgatgcgcc aagtgatgat
    21601 gatatcttta tgattaatcg attcgtctca gctgttgaaa gagaaatagg atatcgattg
    21661 aaaattattt caggtggtaa ttcgagcatg ttaccacaac ttttatataa tgatttgggt
    21721 aaaataaatg aattgcgaat aggtgaaacg ttgtttagag gtgttgatac aactacaaat
    21781 caagcgatag ctatgttata tcaagatgcg attacattgg aggctgagat tttagaaatt
    21841 aaacctcgag ttaatacaca aacgcatgaa tcattcatac aagctattgt agatattggg
    21901 tatttagata caaaagtcga taatatttca cctatggatc agcatatcaa tattttaggc
    21961 gcttcgagtg atcatttaat gcttgattta aatggtcaag gacactatca agtaggagat
    22021 catattagtt tttcacttaa ttatgaagca ctttctcatt cgatgtatat gaaaaattta
    22081 cataaggtat atattgatga ttcaaagatt gatacgctac ttcaaaattt tgatgtgaaa
    22141 tctcctgcta tggtgaacca atattaatat atttatcaat aagtctaagt tgacaagtta
    22201 tactacatat cgtttgcacg gttgtatgat aattgttcaa cttagatttt ttgtatttaa
    22261 tgatttatca aattaagtgc aacagttcgt cacataaaac tgcaataggt aatatcagct
    22321 gaattaaaga gataacggtc atgctaaatg gtgtcaattg tattaattca aagatgatat
    22381 agccatgata cattatcagt attttgtcta aggaaatgtg ctaattgtag tcataattat
    22441 tagaggaaat aaatatagtc atacatttta gtaatataaa aagattgaac gctacttgac
    22501 aatgataatt gttatcaata aaataataaa tgaagttata catattaagg agtggaacga
    22561 tgagaggtct aaaaactttt agtatattgg gattaatagt tgccttattt ttagttgcag
    22621 cttgtggtaa tacggataat tcaagtaaaa aagaatcatc aactaaagat actatttcgg
    22681 taaaagatga aaatggtaca gtaaaagtac ctaaagatgc aaaacgtatc gttgtattag
    22741 agtactcatt tgcagatgca ttagcagcat tagacgttaa accagttggt attgctgatg
    22801 atggtaagaa aaaacgtatc attaaaccag ttagagaaaa aattcgggat tatacttctg
    22861 taggtacacg taaacagcca aacttagagg aaattagtaa attaaaaccg gatttaatta
    22921 tcgctgatag cagtagacat aaaggtatta ataaagaatt aaacaaaatt gcaccaacat
    22981 tatcattaaa gagttttgat ggtgactaca agcaaaatat cgatgctttc aagacaattg
    23041 cgaaagcttt agataaagaa aaagaaggcg aaaaacgtct tgctgaacat gataaattaa
    23101 tcaataagta taaagatgaa attaaatttg atagaaatca aaaagtgctt ccagcagttg
    23161 ttgcgaaagc tggtttatta gcacatccaa actattcgta tgttggacaa tttttaaacg
    23221 aacttggatt taaaaatgca ttaagtgatg atgtaacaaa aggtttaagt aaatacttga
    23281 aaggacctta cttacaatta gatactgaac atttagctga cttaaatcct gaacgcatga
    23341 ttattatgac agataatgct aaaaaagatt ctgctgaatt caagaagtta caagaagatg
    23401 caacatggaa aaaattgaat gcagttaaaa ataatcgtgt tgatattgtt gaccgtgatg
    23461 tttgggcaag atctcgtggc ttaatttctt ctgaagaaat ggctaaagaa cttgttgaat
    23521 tatcaaaaaa agaacaaaag taaggtggaa gtaaatggct ataaaagaat taagtagcca
    23581 atctgccata gatcataaaa gaaaaagacg cacaacactc acgtatatgg tgagtgtgtg
    23641 ctttcttttt atttgtgtat atttaaatat ggcaattggt tcttcaaaaa ttaattttag
    23701 cgatatcgtt cactatgtta ctggtcatac ggatacaaaa gcaacgtttt tattacataa
    23761 tgtacgtatg ccaaggatga ttgcagggtt atttattggc ggtgcgttag cggtatctgg
    23821 tttgttaatg caagcaatga ctcgtaaccc actggcatct ccaaagattt ttggtgttag
    23881 ttctggcgcg tcatttgtca ttgtattagt aacgattatt attccatcat tagaatacta
    23941 ctcactttat ttaggtgtca ttggcgcctt tattggtgga ttaactgtct acacactttc
    24001 tggtgctaca aaaggtatga cgcctattaa attagcgttg gctggtatgg ccattcattt
    24061 attttttagt agtatgactg aagggattat aatcttaaat gaaaattcta atgaacaagt
    24121 gatgttttgg cttgttggtt cattatccag tatgaagtgg gatgagattt taaccattct
    24181 accatggctt attggtgcat taattgcaac agtctttatt ggtcgtcagt taacgataat
    24241 ggaacttggc gatgatattg ctaagggatt gggtcaaaat ataaataaag ttagaatcat
    24301 cattggacta ttagttatta ttcttacagg tatgtctgta tcaatagcag gaccaattgg
    24361 ctttgttggt ttaattgtgc cacatatcgt caagcgctat gtgagtaaga attatttagt
    24421 catgattcca ttaacattta ttataggtgc agatttatta ctactatcag atgtattaag
    24481 tcgtttaatt acatatcctt atgaatcacc agtagggatt gttacatcat tcgtaggtgc
    24541 actttacttc ttatttataa cgattaaagg ggtgaaaaga atatgatgac aacgaaaatt
    24601 atacgtcgtt atagtttaat cacaatcctt ttaattgtga gtatattcat aagtttatgt
    24661 gtagggtctg tcatgattca cccgattgat gctattaagg gaatctttac acaagatgat
    24721 tttattttaa atgagtatcg tatacctaga acgttacttg gtattattat cggtagtagc
    24781 ttggctatat caggtgcaat tatacaagct gtaataagaa acccgttagc ctcacctgat
    24841 gttataggta tttctaaagg tgccagttta gctgctgtga ttattattat gatctttcca
    24901 acagcaccat tgtttgtatt acctataggt tcattcttag gtgcctttgc agtaagttta
    24961 tttttatcat ttttaatttc aaaatatgat gttaaaggtt caaagcttgc cttaattggt
    25021 ttagcaatag gcgcaatatg tacggcgatt gtgcagttct tattaatcag aaatccactg
    25081 gaagcaaaca aagcgcttgt ttggttgacg ggtagtctat atggacatga tatgtcgaat
    25141 atttatacta tattaccttg gtttttaata acgataccaa tcatcatttt attgagttat
    25201 caattagatg tattgaatct aggtgatgct gttgcaaccg cactcggttt aaaagttaaa
    25261 actattaaaa tgctattgct aattttagca gtaatattag caggatccgc aatttcagtt
    25321 gtcggtggta ttagcttttt aggattaatt gcacctcata ttgcgagaca gttaatagga
    25381 aataaaacat tacacgttat tatcatgtcg gggttaattg gtgctatatt gttaacattt
    25441 ggtgatggtt tagcccgtgg cattcatccg ccactggata ttcctgtagg ggttatcatt
    25501 gcaattatcg gtgcaccata cttcttaata ttattacgaa gaatgaaata actaacatat
    25561 gattagagtt tataaaaggt taagtacgaa atcagtaatt gtacttaacc ttttttgaat
    25621 tcatcttgaa aaattacaag aaatagcgta tatttaataa ctataattat tcattatttt
    25681 gcatacataa acattttaaa agatataatg aaaaggaata tgtttgttaa ctgaatagaa
    25741 ttctgtatat acttaaaacg aatacataga gaaatggatt gattttatgg atagcttttc
    25801 aaggaaagag atagtcattg ggagacttaa gtttattacg atgtctttaa ttggtatctt
    25861 attatttcta gtaccaattc cagtcgaaca agatggacag aagcaaacaa cgttgccagt
    25921 ggcattttta gcaggcgttc ttaaagatgt attaggtggc gtaatgccat ttttaattgt
    25981 gacgataatt actttgtcgg gtatcataac actgatttgt tcaactattt taaaagataa
    26041 attgaaacct gatggattat tgcataatgc gtttaatgta agaataggtt ggttaatttt
    26101 gcgattactt gccgttgtct ttgcgtggat gacattttta cgaattggtt caaaagtaat
    26161 atattcagat gaaacgggtg gattattgtt ctcgagttta ttgccaactt tagtggcagt
    26221 attcttattt gcggcattat ttttgccact attaatggaa tacggtttgt tagagatgtt
    26281 gggtcctatt tttagaccgg ttatgagacc tttatttaca ttgccaggac gttcgacggt
    26341 agataattta gcatcattta ttggagacgg aactgttggg gtcttaatta caagtagaca
    26401 atatggtgaa ggatattatt ctcgtcgtga ggcaacagtt atttcaacga catttagtgt
    26461 agtttctatc acctttgcaa ttgtagttgc tgaaacagta catatgcaaa atcaattttt
    26521 tgctttctat ttatcagtga tagtatcctg cctcgttgca gcagtcatta tgccaagaat
    26581 ttggccttta aataaaattc ctgatgagta tgctaaggag gtacctgaaa gtgcgcgtac
    26641 tgaggcattg ccagaaggaa agacggcatt gagacatggc tttgatactg caacagaagt
    26701 aggtattaaa gcaccgggca ttgttgactt ctttaagtca ggattgaaaa cggttgttga
    26761 tatgtggttt gttattttac ctgttgtgat gagtattggt actattgcta ccattattgc
    26821 gaactacacg ccattgttcg ttattttggg taaacctttt gttccatttt tagagttaat
    26881 gcatatacct gaagcggcac aggcttctca aacgattatt atcggttttg ccgatatgtt
    26941 tttaccttct atattaattg aaggtgtaca aaatgatata actcgttttg ttattggggc
    27001 acttagtata tcgcagttaa tatacttgtc tgaagtaggt ggcgtcatac ttggttcgaa
    27061 gattcctgta agcatcggca aattgtttat gattttcttg attagaacaa tcattacact
    27121 accaattatt tctttgatgg cacacttgtt attgtaatta gatatagaaa aggacaactg
    27181 ttaaaattgt gaaaacagtt gtccttttgt atttgtttat gctgggtgtt gaatcaatta
    27241 aaaaaatcat tcatgaagcc ctcaccatga atgatttttt tatacaattt atttagttaa
    27301 ttcattgtaa actttttgat cattttgagt ataaataatg tattcgtggt cttcagtatc
    27361 gataataacg cgattcgttt taccaaatgt tgagccgatt ctagatacat ctttgctgtc
    27421 taatttagga actgcgtgaa tatcttggtc ttgtgtaata tttttaattt cacttgtagg
    27481 aattttgata tcagcaactc tccattgaat acgcacttca ttattatcct tttttactgt
    27541 cattgccatt attcaaacac atcctttaaa tattttgtaa atacatcata aacgattttg
    27601 taatcgtttt caagagttat gtgatacttt tttaaattgt gtcatactaa tgaagttcaa
    27661 cccgaaaata tgttcgtgtt tttggcgaat ttatggtaaa atatgagtat taaaggagaa
    27721 caaatatgac aggtaaaaca catgcttcat gcggcatgct cgttggagcg ctaacaacac
    27781 aatattttca aacggatatt ttttcgtcgg ttacggttat cattctagca actctagcta
    27841 gcttattacc cgatatatgt cacactcaaa gtaagattgg cagaaggttt aaggtaatta
    27901 gtttttttgt gagattgatc tttggacatc gaacctttac gcattcaata ttatttatcg
    27961 caatcatagc atttttattg caaattattc agacgcctaa ttattatatg gcagcaatta
    28021 taattggatt agtgtcacat gttatactag atatgataac accaaaaggc gtgaaattat
    28081 tttatccgtt accgtttaat gtgaagacac cgattcaatt taaaacaggt ggacttgtag
    28141 acttatcttt agctactgcg ttaatgatcg gaacagttta tgttttattt cagcctttca
    28201 tcaatgatat aataagcaac tggaatacta agtttttata gaaaataaat tattaaaggt
    28261 tacaaactca gcaggaggca taaaatgcta gataaaaatc aattagctaa atataaacaa
    28321 gatcatttat gtgaatatga aaaaataatg agtaacaatg aaaaagaagc acttgaagag
    28381 aaggtagcgt cattagattt agattttatt gccaaattat ataacgattt atacatcaat
    28441 aagaaaacga tagatgatgt atctgcagtt tctgaagtga aatatgatat taaatcacaa
    28501 atgagtgatg atgagattaa gcgtttagag gaacaaggac ttcaagctat taaagaagga
    28561 caatttgcag tacttttgat ggcaggtgga caaggaacaa gacttggata taaaggacct
    28621 aaaggttcat tcgaaattga aggtgtaagt ttatttgaac ttcaggcaaa acaattaaaa
    28681 gaactacatc gacaaactgg tcataaaatt caatggtaca ttatgacaag tgatatcaat
    28741 catgaagaaa ctttagctta ttttgaatca cataactact ttggttatga tcaagaatca
    28801 atccatttct ttaagcaaga taatattgtg gcgcttagtg aagcaggaca attaatttta
    28861 aatcaacagg gtcgtattat ggaaacgccg aatggtaatg gtggcgtgtt taaatcttta
    28921 gacaaggcag gatacttaga agaaatgtct aataatggcg ttaagtatat tttcttgaat
    28981 aatatcgata atgttttagt aaaagtgttg gatccactat ttgcaggatt tacggtagaa
    29041 catgattacg atattacttc taaaacgatt caaccaaaac caggagaaag tgttgggcga
    29101 ttagttaatg tcgattgtaa agatacagta ttggagtatt cagaattaga tccagaagtt
    29161 gcaaatcaat ttaacaatgc taatatcggt attcatgcat ttaaacttgg ttttatttta
    29221 aatgctgtaa atcgtgaatt accttatcat ttagctatta agaatttgaa gcaattagat
    29281 gagaactttg gtgttattga acaaccaact ttaaaattcg aattgttcta ttttgacata
    29341 tttacttatg gaactagttt tgtcacgtta caagttccta gagaagaaga gttctcacct
    29401 cttaaaaata aagaaggtaa agatagtgtt gcaacagcga ctgaagattt acgtagaatg
    29461 ggtttaattt aatgttaggc ggtgacttac gtgtccaaat ctagtgaaaa aagtaatgac
    29521 acaatcgtag aaacatttaa ggatatcatc cctttatcat ttggagaaga aattggtaat
    29581 gcagcatctc atggtgttgc agctttgtta acattattag tgttacccta tgcagctgtg
    29641 cacagttata ttgcatatgg tacgcttgcg tctgtgagta tgtctattta tgttatttct
    29701 atctttatga tgtttatttc atctactatt tatcattcta tgcaaaatga aacgcctcat
    29761 aaatatattt taagaattat tgatcatagt atgatttatg tagcaatttc aggtacatat
    29821 acgcctattt tattaacagt tgtaggtggt tggattggat ggacagtgtt tatcctatta
    29881 tggggcacaa cgctttgggg aattttatat aaatccatag ctgtcaaagt taaccaaaag
    29941 ttaagcttaa ttgtctattt agtgatgggc tgggtaggta ttatcttgtt accaattatt
    30001 tttattcgca catcatggat attcatatta ttcattttct taggcggtgt gtcatatacg
    30061 attggtgcat ggttctatgc acaaaaaaac agaccatatt ttcatatgat atggcatatt
    30121 tttattgtta tagcatcatt attgcattta attgctattt tatattttat gtaatagttt
    30181 atcagaaggt ggggggaact ccgctttttt tattacttaa ttcattctaa tagaggattg
    30241 ttttgtatga ctattatcgt taagatttga aagtcaaaag tgtttataca aatacaaatc
    30301 aatagtttgt aaataaaatc gtataaatat atataaagaa ggaggtatag catgcgttta
    30361 aaatcaattg taactgtaat agctttaata ttaatcatgt ttatggctgc aatcgaatca
    30421 tcaattattt ctttagcgtt accaacaatt aagcaagatt taaatgcagg gaatttaatt
    30481 tcattaattt ttaccgcgta ttttatcgca ttagtaattg ccaatcctat agtaggtgaa
    30541 ttattaactc gttttaaaat tatttatatt gctatcgcag gtcttacact attcacggtt
    30601 ggtagtttaa tgtcaggttt aagcacacat tttagtatgt tggttatatc acgtgtaatt
    30661 caagggtttg gatctggtgt catgatgtcc ttgtcacaaa ttgtgcccaa attagcattt
    30721 gaaattccat tacgctataa aattatgggt atcgttggta gcgtttgggg gatttcgagt
    30781 atcattggac cacttctagg tggtggcata ttagaatttg caacatggca ttggttattc
    30841 tacataaaca taccgattgc gattattgca ataatattgg ttgtctggac atttcatttt
    30901 ccagaagaag aaactgtggc taaatcaaaa ttcgatacta aaggtatcac cctgttttat
    30961 attttcattg gtttaattat gtttgcattg ttaaaccaac aacatttata tttaaatatc
    31021 ataggttttg tcttagcaat attagttgca cttcgattat ttaatgttga gaaaaaagtt
    31081 tcatccccat tcttaccagt agcagaattt aatcgaatga ttactttggt ctttattaca
    31141 gatcttctta cagctgtttg tttaatgggc tttaatttat atattcctgt gtatcttcaa
    31201 gaacaactgg ggttatcgcc attgcaaagt ggattagtta tttttccact ttctgtagcg
    31261 tggattacat tgaattttaa tttgcaccat attgaagcga agttatcaag aaaagttatc
    31321 tatttgtcgt cttttacatt attattatta agtagtataa tcattgcgtt tggtataaaa
    31381 ttaccaattc ttatagcatg tgtcttaatt ttaagtggac taagctttgg atatatttat
    31441 acaaaagata gtgtaattgt ccaagaggaa acaagtccaa tacaaatgaa gaaaatgatg
    31501 tcgttttatg gattaacaaa aaaccttggc gcatcaatag gttcgacgat tatgggatac
    31561 ctttatgcgt taaaatcagg attgtttggt gcaaacttgc ataatatttt aggcgttgta
    31621 tccttgataa gtgtagggct catcgttgta tggctcatat tttatagaga agcggcgtca
    31681 caaacaaaag aatagaatta tgattaaaat tgatgtagtg taaataaagt aacaaataat
    31741 cattttacta agtttgaagt gtaatcaact atcttaaaat taaaagcaat ataaattatt
    31801 tatggtaagg aggacaatag ttatgaatta tcttaaacat aagttttata atttattaac
    31861 aacaattatt gttctcttta tctttgtgct ttctggtgcg atattcttaa cgtttttagg
    31921 gttcggacta tatggattaa gtagaattct tatttatttt aggttaggtg acttcacata
    31981 taacagaagt atgtacgaca atttgctata ttatggtagt tatattatat ttggctactt
    32041 tatcgtattt gcagttgaac atttgatgga ttattttaga aaaatgttac ctgaaaatgc
    32101 ttattttaga ggtgcaactt ttcatttaat ctcttatatg gttgcaacaa cgttatttta
    32161 ttttattatt catctgaact atgtgtatat caacattgat ttttgggtca tcatggttat
    32221 tataggcttt ttatatgttt gtaagctgca gttttaccct gaaagtaaaa atctaaataa
    32281 tagaaaatag agttttgaag ttgggggcaa tgatgtgttc caacttcttt ttattcatat
    32341 agtagaaaat gagttgtttt gcttcaatta atcacggtta ataataacca ttgatataaa
    32401 tagttggtac tttaatctga aatatatttt aagtatatat gctaataaac gtaaatttgt
    32461 tacaaaggag gaattatgat ggctaaagtt gaattaacaa cccggagacg aaacttcata
    32521 gttgctgtta tgttgattag tgcgtttgta gctattttaa atcaaacatt attaaataca
    32581 gcgttaccta gtataatgag agaattaaat attaatgaaa gtacatcgca atggctagtt
    32641 actgggttta tgcttgttaa tggcgtcatg atacctctaa cagcatactt aatggacagg
    32701 attaaaacta gacctttata cttagcagcg atggggacat ttttagtagg atcaattgtt
    32761 gcagccatgg cacccaattt tggagtttta atgttagcac gtgtcatcca agctatgggg
    32821 ggcaggcgta ctcatgcctt taatgcaatt cacattgttt acgttattca gtaaagaaca
    32881 tagaggattt gcaatgggat tagctggttt agttattcaa tttgcaccag caatcgggcc
    32941 tactgttact ggattaatca ttgatcaagc gagttggcga gtaccattta ttataattgt
    33001 aggaattgct ttagtcgcat tcatcttcgg tttaatttca atttcaagct ataatgaagt
    33061 gaaatatact aagctagata agcgctctgt aatgtattca acgattggat ttggattaat
    33121 gttatatgca ttcagtagtg caggcgacct aggatttact agcccggtag tcattggtgc
    33181 aatgataatt agttctatta tcatctactt atttattcgt agacaattca atataagcaa
    33241 tgcactttta aatttaagtg tttttaagaa tagaacattt gcattatgta cgattagttc
    33301 aatgattata atgatgtcaa tggttggtcc agcgttatta ataccgctct atgttcaaaa
    33361 tagcttagct ctatctgcat tattgtttgg acttgttatc atgccaggtg caatcataaa
    33421 tggtattatg tcggttttta caggtaaatt ttatgataag tatggtccta gaccactgat
    33481 ttatactggt tttacgattt taacaattac tactattatg ttgtgtttct tgcatacaga
    33541 cacatcttat acgtatttaa tagtcgtata tgcaattaga atgttttcag tttctttact
    33601 catgatgccg ataaatacta caggaattaa ttctttgaaa aatgaagaaa tctcacatgg
    33661 cacggctatt atgaactttg gtcgtgtaat ggctggctca ttaggaacag cattaatggt
    33721 tactttgatg agttttggcg caaaaatatt ctcatctaca tcgccatcgc atttaactgc
    33781 aactgaaatt aaacagcaat caattgctat aggggtggat atctcatttg cttttgtagc
    33841 tgtgcttgtt atggcagctt atgtgatagc gctttttata agagaaccta aagaaataga
    33901 aagtaataga aggaaatttt aaaataatta tagtagttgg tctattgaaa ataataggct
    33961 aacttctttt tttatttaat gaaaagtttt atacttttat tgatagacta agcaaaaatt
    34021 gttatttgct atgatgtaga tattttaaat tgattaaggg ggatttgctt tgttaacggt
    34081 agatcaagtt aaagaactaa taggagaaat taaagatcct attatagatg tacctttaaa
    34141 agaaacagaa ggtattgtag aagtttctat taaggaagaa aaagaacatg taagtgttaa
    34201 agtggcaatg gcacaattag gtggtgcacc gcaattagat ttacagatgg cagttgttaa
    34261 tgttttaaaa gaaaatggtg caaaaactgt gggtatacga tttgaaacgt taccagaaga
    34321 aaaagtaaat caatttaaac caaaagaaga aaataaacct aaaacgatag aaggcttatt
    34381 atctcaaaat aatccagttg aatttattgc tattgcttca ggtaaaggtg gcgtcggtaa
    34441 atctactgtt gcagtaaatt tagccgttgc cttagcccgt gaaggtaaaa aagttggatt
    34501 agtagatgcc gatatatatg gatttagtgt accagatatg atgggtattg atgaaaagcc
    34561 tggaattaat gggaaggaag taattccagt tgaacgtcat ggcgttaaag ttatatcaat
    34621 ggcatttttt gtggaagaaa atgcaccagt tatatggaga ggaccaatgt taggtaaaat
    34681 gttgacgaat ttctttacag aagttagatg gggagacatt gaatatttaa tactcgatct
    34741 tccacctgga acaggagatg tagctttaga tgttcatacg atgttacctt caagtaagga
    34801 aattattgta acgacacctc atcctacagc agcatttgtt gcagctcgcg caggtgcgat
    34861 ggcaaaacat acggatcatt ctattcttgg cgtaattgaa aacatgagtt attttgaaag
    34921 taaagagacg gggaataaag aatatgtctt tggcaaaggt ggcggtacta agttagctga
    34981 tgaacttaat actcaattac ttggggaatt acctttagag caaccatctt ggaatccaaa
    35041 agattttgca ccgtcaatat atcaatcaga tgatcgtcta ggtaaaattt atagctcgat
    35101 tgcacaaaaa gttatagctg ccacaaataa ataggtataa tatatgctaa acgtacaaaa
    35161 ttacttttat tttgtacgtt tttatattga tttgttttgg aatgctggta aaataagttc
    35221 ttgtgttaag caataaacgc attgaaaaaa gaagcgactt gcaaacacga atgtttttca
    35281 aaaaagttat tgactttaaa ttataaagtt gataagataa taaagtcgtc aaaaacggtg
    35341 atgatgtttt taaagaagaa tttcaaaagt gtaaaactta ccattgaaat aactataaaa
    35401 acaatataaa ataaataaag taatcgttcg aaaagttatt gacttcaata attaaatgaa
    35461 gtacaataaa agagattgaa cattaatgaa cattgaaaac tgaatgacaa tatgtcaacg
    35521 ttaattccaa aaaacgtaac
//
LOCUS       DATTQF010000015        35221 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.015, whole genome shotgun sequence.
ACCESSION   DATTQF010000015 DATTQF010000000
VERSION     DATTQF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 35221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.015"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..303)
                     /locus_tag="VB241_000601"
     CDS             complement(<1..303)
                     /locus_tag="VB241_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243925.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFV"
     gene            660..968
                     /gene="rpsJ"
                     /locus_tag="VB241_000602"
     CDS             660..968
                     /gene="rpsJ"
                     /locus_tag="VB241_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP1243926.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            996..1658
                     /gene="rplC"
                     /locus_tag="VB241_000603"
     CDS             996..1658
                     /gene="rplC"
                     /locus_tag="VB241_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP1243927.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1685..2308
                     /gene="rplD"
                     /locus_tag="VB241_000604"
     CDS             1685..2308
                     /gene="rplD"
                     /locus_tag="VB241_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP1243928.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKVQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            2308..2583
                     /gene="rplW"
                     /locus_tag="VB241_000605"
     CDS             2308..2583
                     /gene="rplW"
                     /locus_tag="VB241_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP1243929.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2616..3449
                     /gene="rplB"
                     /locus_tag="VB241_000606"
     CDS             2616..3449
                     /gene="rplB"
                     /locus_tag="VB241_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP1243930.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3516..3794
                     /gene="rpsS"
                     /locus_tag="VB241_000607"
     CDS             3516..3794
                     /gene="rpsS"
                     /locus_tag="VB241_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP1243931.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            3823..4176
                     /gene="rplV"
                     /locus_tag="VB241_000608"
     CDS             3823..4176
                     /gene="rplV"
                     /locus_tag="VB241_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP1243932.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            4200..4853
                     /gene="rpsC"
                     /locus_tag="VB241_000609"
     CDS             4200..4853
                     /gene="rpsC"
                     /locus_tag="VB241_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP1243933.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            4856..5290
                     /gene="rplP"
                     /locus_tag="VB241_000610"
     CDS             4856..5290
                     /gene="rplP"
                     /locus_tag="VB241_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP1243934.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            5280..5489
                     /gene="rpmC"
                     /locus_tag="VB241_000611"
     CDS             5280..5489
                     /gene="rpmC"
                     /locus_tag="VB241_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP1243935.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5513..5776
                     /gene="rpsQ"
                     /locus_tag="VB241_000612"
     CDS             5513..5776
                     /gene="rpsQ"
                     /locus_tag="VB241_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP1243936.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            5808..6176
                     /gene="rplN"
                     /locus_tag="VB241_000613"
     CDS             5808..6176
                     /gene="rplN"
                     /locus_tag="VB241_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP1243937.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            6212..6529
                     /gene="rplX"
                     /locus_tag="VB241_000614"
     CDS             6212..6529
                     /gene="rplX"
                     /locus_tag="VB241_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP1243938.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6556..7095
                     /gene="rplE"
                     /locus_tag="VB241_000615"
     CDS             6556..7095
                     /gene="rplE"
                     /locus_tag="VB241_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP1243939.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            7118..7303
                     /locus_tag="VB241_000616"
     CDS             7118..7303
                     /locus_tag="VB241_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP1243940.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7335..7733
                     /gene="rpsH"
                     /locus_tag="VB241_000617"
     CDS             7335..7733
                     /gene="rpsH"
                     /locus_tag="VB241_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP1243941.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            7758..8294
                     /gene="rplF"
                     /locus_tag="VB241_000618"
     CDS             7758..8294
                     /gene="rplF"
                     /locus_tag="VB241_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP1243942.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            8325..8684
                     /gene="rplR"
                     /locus_tag="VB241_000619"
     CDS             8325..8684
                     /gene="rplR"
                     /locus_tag="VB241_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP1243943.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8705..9205
                     /gene="rpsE"
                     /locus_tag="VB241_000620"
     CDS             8705..9205
                     /gene="rpsE"
                     /locus_tag="VB241_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP1243944.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            9222..9401
                     /gene="rpmD"
                     /locus_tag="VB241_000621"
     CDS             9222..9401
                     /gene="rpmD"
                     /locus_tag="VB241_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP1243945.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9418..9858
                     /gene="rplO"
                     /locus_tag="VB241_000622"
     CDS             9418..9858
                     /gene="rplO"
                     /locus_tag="VB241_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP1243946.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            9858..11150
                     /gene="secY"
                     /locus_tag="VB241_000623"
     CDS             9858..11150
                     /gene="secY"
                     /locus_tag="VB241_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP1243947.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            11167..11814
                     /locus_tag="VB241_000624"
     CDS             11167..11814
                     /locus_tag="VB241_000624"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP1243948.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            12007..12225
                     /gene="infA"
                     /locus_tag="VB241_000625"
     CDS             12007..12225
                     /gene="infA"
                     /locus_tag="VB241_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP1243949.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            12257..12370
                     /gene="rpmJ"
                     /locus_tag="VB241_000626"
     CDS             12257..12370
                     /gene="rpmJ"
                     /locus_tag="VB241_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP1243950.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            12393..12758
                     /gene="rpsM"
                     /locus_tag="VB241_000627"
     CDS             12393..12758
                     /gene="rpsM"
                     /locus_tag="VB241_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP1243951.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            12782..13171
                     /gene="rpsK"
                     /locus_tag="VB241_000628"
     CDS             12782..13171
                     /gene="rpsK"
                     /locus_tag="VB241_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP1243952.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            13246..14190
                     /locus_tag="VB241_000629"
     CDS             13246..14190
                     /locus_tag="VB241_000629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP1243953.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            14207..14575
                     /gene="rplQ"
                     /locus_tag="VB241_000630"
     CDS             14207..14575
                     /gene="rplQ"
                     /locus_tag="VB241_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP1243954.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            complement(14828..16395)
                     /locus_tag="VB241_000631"
     CDS             complement(join(14828..15720,15720..16395))
                     /locus_tag="VB241_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611580.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSau2 family transposase"
                     /protein_id="HEP1243955.1"
                     /translation="MKTLKGALFMSIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLD
                     INVNTLYPKYKYYKTHGIETLLTQKQYNHYSKELKLKVVNEYLNSNQSTQDIAIKYNI
                     RSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREV
                     AEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMTPEEQKEAEIQALKAQNRL
                     LEMENDVFKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWV
                     HYQSSELELENEQLKREIESIYHKYNGIYGYRRIYIYIRLKLGKQVNRKRIYRLMKEL
                     NLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSA
                     IYDLGAKRIVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHM
                     LDECGIIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFILF
                     FNHERITLKMADSV"
     gene            16771..17580
                     /locus_tag="VB241_000632"
     CDS             16771..17580
                     /locus_tag="VB241_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP1243956.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALEQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            17577..18437
                     /locus_tag="VB241_000633"
     CDS             17577..18437
                     /locus_tag="VB241_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP1243957.1"
                     /translation="MIIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKTIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKHQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            18427..19233
                     /locus_tag="VB241_000634"
     CDS             18427..19233
                     /locus_tag="VB241_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1243958.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWMFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            19238..20032
                     /gene="truA"
                     /locus_tag="VB241_000635"
     CDS             19238..20032
                     /gene="truA"
                     /locus_tag="VB241_000635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP1243959.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRIHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTDLNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQQRDPFQSGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSL"
     gene            20281..20718
                     /gene="rplM"
                     /locus_tag="VB241_000636"
     CDS             20281..20718
                     /gene="rplM"
                     /locus_tag="VB241_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP1243960.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            20732..21130
                     /gene="rpsI"
                     /locus_tag="VB241_000637"
     CDS             20732..21130
                     /gene="rpsI"
                     /locus_tag="VB241_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP1243961.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            21479..22915
                     /locus_tag="VB241_000638"
     CDS             21479..22915
                     /locus_tag="VB241_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEP1243962.1"
                     /translation="MTSSKYIEYLQTEVEGQLLDRKRASINPKDVAQHISAFANAEGG
                     KLVIGIEDNGEISGFNHSKAKKKEVYIEAPFEHLYRIPKYTYEIIEVKKRDGTMDEIL
                     IFDIEPSYDAIITLKDDSVYLRINDKSRKLTHNQITNLEYDRGSRRFEEELVEYSSIE
                     DVNEELVDEFKQLLNTNVDNEKLLKARGFMREGKLTVAGLLLFSNHINVYLPGARIRF
                     MRYEGTKEESGTRLNIVKDITFDKALPVAIREARAFINTQLREYTFLGKEGRFVTLPE
                     YPEFAWFEGMINSIVHRRYDNQGDHIRIKMFDDRLEISSPGALPASVTLENIKEERYS
                     RNPKLAAALTQYKWVRESNEGVGRIFDEMKQYFLDDPVYSEPNNSSVQLALKNNIIAR
                     KKRETGRVSNIITPELFESLNEQQELIVRHLYNQGELTASKIANIIQRSVPTARKRLK
                     ELEEMNIISTHGSSKNDPKRTYYLLGFE"
     gene            23164..23343
                     /locus_tag="VB241_000639"
     CDS             23164..23343
                     /locus_tag="VB241_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243963.1"
                     /translation="MTEIVEFLSDGKLASRLEIQETMGLQKSYTSELIAELKKLEIIG
                     SEGRGPGTRYYLIRD"
     gene            23993..24175
                     /locus_tag="VB241_000640"
     CDS             23993..24175
                     /locus_tag="VB241_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243964.1"
                     /translation="MIDSVFWKKAFIPVYFIVAMLVFLLFRFYIKTNNFSIYLMIIFL
                     MCLGTASIIYNYKNNR"
     gene            complement(24157..24412)
                     /locus_tag="VB241_000641"
                     /pseudo
     CDS             complement(24157..24412)
                     /locus_tag="VB241_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24645..26309
                     /gene="alsS"
                     /locus_tag="VB241_000642"
     CDS             24645..26309
                     /gene="alsS"
                     /locus_tag="VB241_000642"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEP1243965.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            26346..27050
                     /gene="budA"
                     /locus_tag="VB241_000643"
     CDS             26346..27050
                     /gene="budA"
                     /locus_tag="VB241_000643"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEP1243966.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGAVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(27441..27866)
                     /locus_tag="VB241_000644"
     CDS             complement(27441..27866)
                     /locus_tag="VB241_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HEP1243967.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNQLASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            28161..28976
                     /locus_tag="VB241_000645"
     CDS             28161..28976
                     /locus_tag="VB241_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEP1243968.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TVVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILHHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDRRILEVLVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            29187..30041
                     /locus_tag="VB241_000646"
     CDS             29187..30041
                     /locus_tag="VB241_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEP1243969.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTW
                     QSDVHELKVCTQNDGTIEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNLGKVNADYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTKESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQGE"
     gene            complement(30101..32527)
                     /locus_tag="VB241_000647"
     CDS             complement(30101..32527)
                     /locus_tag="VB241_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HEP1243970.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTTDYEKLRNIWLDVNYGYDKYDENNPDMKKKFEATENEAEKLLKEMKTESDRKYLWE
                     SSKDLDTKSADMTRTYRNIEKISEAMKHKNTKLKTDENKTKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLQEDFTDEEKKKYTAPIK
                     TFAPDSDKILSSVGKSEPAKGGNLVDISKVKLLESIIEEDKDMMKKSIDSFNTVFTYA
                     QNSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDSNQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDTMDKSTK
                     AKYKKIVKTSVESDSSYKQTDYLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKGLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLDNEEPKSTDVKKSSKTFVGGTKFDDQHASIGMDFKNQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANDYKLYKDDTQTTNSDNQETNSLF
                     LESTNSTQNNIGYHFLNESKITVKKESHTGKWSDINKSQKDIQKTDEYYEVTQKHSNT
                     DSKYAYVLYPGLSKDVFKSKASKVTVVKQEDDFHVVKDNESVWAGINYSDSAKTFEIN
                     NTKVEVKAKGMFILTKKDDNTYECSFYNPESTNSVSDIESKISMTGYSIINKNTSTSN
                     ESGVRFELTK"
     gene            complement(32524..32691)
                     /locus_tag="VB241_000648"
     CDS             complement(32524..32691)
                     /locus_tag="VB241_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243971.1"
                     /translation="MLKIWVIDCLYASTFFVDCLTVDSQLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            32934..33350
                     /locus_tag="VB241_000649"
     CDS             32934..33350
                     /locus_tag="VB241_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1243972.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDNDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            33420..34268
                     /locus_tag="VB241_000650"
     CDS             33420..34268
                     /locus_tag="VB241_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1243973.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYIDL
                     VLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            34489..34614
                     /locus_tag="VB241_000651"
     CDS             34489..34614
                     /locus_tag="VB241_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243974.1"
                     /translation="MTYIPFSIITLITGIIMHMTMYFVKFECRKMPLFMTIIFTI"
     gene            34569..34862
                     /locus_tag="VB241_000652"
     CDS             34569..34862
                     /locus_tag="VB241_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243975.1"
                     /translation="MSENAIIHDDYFYNLKAVKTHNIAKNVNKSLLNDKGVSIGKFIQ
                     KLKGKNPTWRYPKIKWTISKNKGQSYGGSYWKLINNKGKRIASLTKEGKILRE"
     gene            34876..>35221
                     /locus_tag="VB241_000653"
     CDS             34876..>35221
                     /locus_tag="VB241_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1243976.1"
                     /translation="MCFDINNFDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHVVYVYNEPYKRTNVIKKFTSLTIDELYYFKEY
                     KKEKDDNILCEEF"
BASE COUNT        12520 a         5365 c         6545 g        10791 t
ORIGIN      
        1 gacaaataga atcattagga aaaataaaat ccaatgaatc aaaattccta agaaggggat
       61 aatagctaaa aaactaccta ctataccaaa tattggaata atcgctatag gtctaattgc
      121 attttgtcta ttcactaata atataatagt aagaattaga taaagtaatg cattaaagag
      181 taaaggctgc catccaaaac ttaaaataaa cgttgcacct aaaaaaggaa tgccatagaa
      241 aaactcacaa agtattaaaa agatacctat aagtgctaaa gataattttg gtccattttt
      301 catattcatt caccaccgtt cttatgacta attatataga ataattttca atgataataa
      361 taaaatgatt caaggtacga atttacaata aagaaggaaa taaaaaatat ctcgaaaata
      421 gttgaactga ctaagtatat aaagtaaaat ataaaagtat gtgttagaca gatagaaaaa
      481 ttatgtgaaa agccttgatt tatcgatgtt tttcgtgtaa tataactaac gttggacttt
      541 aagaaaagcg atgaagcgaa aggttactga cacacccggc cgctttgcca tggcgctgtg
      601 taagatagtt ttcgtggaga agtctatcac taaatgtaga cgaataagga gggaaaatta
      661 tggcaaaaca aaaaatcaga atcagattaa aagcttatga tcaccgcgtg attgatcaat
      721 cagcagagaa gattgtagaa acagcgaaac gttctggtgc agatgtttct ggaccaattc
      781 cgttaccaac tgagaaatcg gtatacacaa tcatccgtgc cgtgcataag tataaagatt
      841 cacgtgaaca attcgaacaa cgtacacata aacgtttaat cgatattgta aacccaacac
      901 caaaaacagt tgacgcttta atgggcttaa acttaccatc tggtgtagac atcgaaatca
      961 aattataata gacaatttta ggaggtggac tttcgatgac caaaggaatc ttaggaagaa
     1021 aaattgggat gacacaagta ttcggagaaa acggtgaatt aatccctgta acagtagtag
     1081 aagctaaaga aaatgttgta ttacaaaaga aaactgtaga agttgatgga tacaacgcaa
     1141 tccaagttgg atttgaagac aaaaaagcat acaaaaaaga tgcaaaatct aacaaatatg
     1201 ctaataaacc agctgaaggt cacgctaaaa aagctgacgc agcacctaag cgcttcattc
     1261 gtgaattccg caatgtagac gtggatgctt acgaagtagg tcaagaagtc tcagtagata
     1321 cttttgtagc tggcgacgtt attgacgtaa caggcgtatc aaaaggtaaa ggtttccaag
     1381 gtgcaattaa acgccacgga caatctcgtg gacctatgtc acacggttct catttccaca
     1441 gagcaccagg ttctgtaggt atggcttcag atgcttctag agtattcaaa ggccaaaaaa
     1501 tgccaggacg tatgggtgga aacactgtaa ctgttcaaaa cttagaagta gttcaagttg
     1561 acacagaaaa caaagttatc ttagtaaaag gtaatgtacc tggacctaaa aaaggtttag
     1621 tagaaatcag aacttcaatt aaaaaaggta ataaataata agaagtgaaa ggaggaaatg
     1681 cataatggct aattatgatg ttttaaaatt agacggaact aaatcaggtt caatcgaatt
     1741 aagcgatgca gtattcggta ttgagccaaa taatagcgtt ttattcgaag ctattaattt
     1801 acaacgtgct tcattacgtc aaggtacgca tgctgttaag aatcgttcag cagtaagcgg
     1861 tggcggacgt aaaccatgga agcaaaaagg aacaggtcgt gctcgtcaag gtacaatccg
     1921 tgctccacaa tggcgtggcg gtggtatcgt attcggacca actccaagaa gttatgcata
     1981 caaaatgcct aagaaaatgc gtcgtttagc tttacgctca gcattatctt tcaaagttca
     2041 agagaatggc ttaactgtag ttgacgcatt caacttcgaa gctccaaaaa ctaaagaatt
     2101 caaaaatgta ttatctacat tagaacaacc taaaaaagta ttagtagtta ctgaaaacga
     2161 agatgtaaat gttgaattat cagcacgcaa catccctggc gttcaagtga caactgctca
     2221 aggtttaaat gttttagata tcactaatgc tgacagctta gtaattactg aagctgctgc
     2281 taaaaaagtt gaggaggtgc tcggataatg gaagcaagag atattcttaa gcgccccgta
     2341 atcactgaga aatcttctga agcaatggct gaagacaaat acactttcga cgttgatact
     2401 cgtgttaaca aaacacaagt gaaaatggca gttgaagaaa tcttcaacgt aaaagttgca
     2461 agtgttaata tcatgaatta caaacctaag aaaaaacgta tgggccgtta ccaaggctat
     2521 acaaacaaaa gaagaaaagc gattgtaact cttaaagaag gatcaatcga cttatttaac
     2581 taataaacca attaccatta aggaggtaag cgacaatggc tattaaaaag tataagccaa
     2641 taacaaatgg tcgtcgtaat atgacttcgt tagatttcgc agaaatcacg aaaactacac
     2701 ctgaaaagtc attattaaaa ccgctaccga aaaaagcggg acgtaacaac caaggtaaat
     2761 tgactgtaag acaccatggt ggtggacaca aacgtcaata ccgtgttatc gatttcaaac
     2821 gtaacaaaga tggtatcaat gcaaaagttg attctattca atatgatcca aaccgctcag
     2881 caaacatcgc tttagttgta tatgcagacg gtgaaaaacg atatatcatt gctcctaaag
     2941 gattagaagt aggtcaaatc gttgaaagtg gtgctgaagc tgacatcaaa gttggtaacg
     3001 cattaccatt acaaaacatt ccagttggta cagtagtaca caacatcgag cttaaacctg
     3061 gtaaaggtgg acaaatcgct cgttcagctg gtgcaagtgc tcaagtactt ggtaaagaag
     3121 gtaaatacgt attaatcaga ttaagatctg gtgaagttcg tatgatctta tctacttgcc
     3181 gtgctacaat cggtcaagtt ggtaacctac aacacgaatt agttaacgtt ggtaaagccg
     3241 gacgttcaag atggaaaggt attcgtccaa cagttcgtgg ttctgtaatg aaccctaacg
     3301 atcacccaca cggtggtggt gaaggtcgtg ctcctatcgg tagaccatct ccaatgtcac
     3361 catggggtaa acctacgctt ggtaagaaaa ctcgtcgtgg taaaaaatca tcagacaaac
     3421 ttatcgttcg tggacgtaag aaaaaataat ataatcaact tatttgggtg tgcggcttaa
     3481 agctgcacgc acataataag aagggaggcg cccaaatggc tcgtagtatt aaaaaaggac
     3541 ctttcgtcga tgagcattta atgaaaaaag ttgaagctca agaaggaagc gaaaagaaac
     3601 aagtaatcaa aacatggtca cgtcgttcta caattttccc taatttcatc ggacatactt
     3661 ttgcagtata cgacggacgt aaacacgtac ctgtatatgt aactgaagat atggtaggtc
     3721 ataaattagg tgagtttgct cctactcgta cattcaaagg acacgttgca gacgacaaga
     3781 aaacaagaag ataatatcta ttaagtagag gaggacatcc taatggaagc aaaagcggtt
     3841 gctagaacaa taagaatcgc acctcgtaaa gtaagactag ttcttgactt aatcagaggt
     3901 aaaaatgctg ctgaagctat tgcaatttta aaattaacaa acaaagcttc atcaccagta
     3961 attgaaaaag tattaatgtc cgctttagct aatgctgaac ataactatga catgaacaca
     4021 gatgaattag tagttaaaga agcatatgct aacgaaggac caacattaaa acgtttccgt
     4081 ccacgtgcgc aaggtcgtgc aagtgcgatt aacaaacgta caagccacat tacaatcgtc
     4141 gtaagtgacg gtaaagaaga agctaaagaa gcttaattaa cttttaagga gggaatactg
     4201 tgggtcaaaa aattaatcca atcggacttc gtgttggtat tatccgtgat tgggaagcta
     4261 aatggtatgc tgaaaaagac ttcgcttcac ttttacacga agatttaaaa atccgtaaat
     4321 ttattgataa tgaattaaaa gaagcatcag tttctcacgt agagattgaa cgtgctgcaa
     4381 accgtatcaa cattgcaatt catactggta aacctggtat ggtaattggt aaaggcggtt
     4441 cagaaatcga aaaattacgc aacaaattaa atgcgttaac tgataaaaaa gtacacatca
     4501 acgtaattga aatcaaaaaa gttgatcttg acgctcgttt agtagctgaa aacatcgcac
     4561 gtcaattaga aaaccgtgct tcattccgtc gtgtacaaaa acaagcaatc actagagcta
     4621 tgaaacttgg tgcaaaaggt atcaaaactc aagtatctgg tcgtttaggc ggagctgaca
     4681 tcgctcgtgc tgaacaatat tcagaaggaa ctgttccact tcatacgtta cgtgctgaca
     4741 tcgattatgc acacgctgaa gctgacacta cttacggtaa attaggcgtt aaagtatgga
     4801 tctatcgtgg agaagttctt cctactaaga acactagtgg aggaggaaaa taataatgtt
     4861 actaccaaaa cgtgtaaaat atcgtcgtca acatcgtcct aaaacaactg gtcgttctaa
     4921 aggcggtaac tacgtaacat ttggtgagtt tggtttacaa gctacaacaa cgtcttggat
     4981 cacatctcgt caaatcgaat ctgctcgtat agcaatgaca cgttacatga aacgtggcgg
     5041 gaaagtttgg attaaaatct tcccacatac accatatact aaaaaacctt tagaagtacg
     5101 tatgggtgct ggtaaaggtg cggttgaagg ctggatcgca gttgttaaac caggtagaat
     5161 tttattcgaa gttgctggcg tttctgaaga agttgcgcgt gaagcactac gtttagcaag
     5221 tcacaaactt ccagtaaaaa ctaagtttgt aaaacgtgag gaattgggtg gtgaaacaaa
     5281 tgaaagctaa ggaaattaga gacttaacca cttcagaaat cgaagaacaa atcaaatctt
     5341 caaaagaaga gctttttaac ctacgctttc agttagctac aggtcaatta gaagaaactg
     5401 cacgtattcg tacagtaaga aaaacgattg cacgtctaaa aactgttgct cgtgaaagag
     5461 aaattgaaca aagtaaggct aatcaataat taagtaagag gaggttacaa aagtgagcga
     5521 aagaaacgat cgtaaagttt atgtaggtaa agttgtttca gacaaaatgg acaagactat
     5581 tacagtactt gttgaaactt acaaaacaca caaattatac ggtaaacgag tgaaatactc
     5641 taaaaaatac aaaactcatg atgaaaacaa ttcagctaaa ttaggagaca ttgttaaaat
     5701 tcaagaaact cgtcctttat cagcaacaaa acgttttcgt ttagtagaga ttgttgaaga
     5761 gtcagtaatt atttaataca agtttagaga taagggaggt ttaactaatg atccaacaag
     5821 aaacacgctt gaaagtagca gacaactctg gtgctcgtga agttcttaca atcaaagtat
     5881 taggtggatc tggtcgtaaa acagcaaaca tcggcgatgt tatcgtatgt actgttaaaa
     5941 atgcaacacc aggtggcgtt gttaaaaaag gtgacgttgt caaagctgta atcgtacgta
     6001 ctaagtcagg tgttcgtcgt aatgacggtt catatatcaa atttgatgaa aatgcatgtg
     6061 ttatcatccg tgatgacaaa ggcccacgtg gtactcgtat cttcggacct gttgctcgtg
     6121 aattacgtga aggtaacttc atgaaaatcg tatcattagc accagaagta ctttaattaa
     6181 taacaaccaa tacaaataag gaggtgccca catgcatatc aaaaaaggtg acaacgttaa
     6241 agttatcgca ggtaaagaca aaggtaaaga aggtaaagta atcgctactc tacctaaaaa
     6301 agaccgtgtc gttgtggaag gtgttaacat tatgaaaaaa caccaaaaac caactcaatt
     6361 aaatcctgaa ggtggaatct tagaaacaga ggcagcaatc catgtttcta atgtacaatt
     6421 attggaccct aaaacaaacg aaccaactcg tgtaggttac aaatttgttg atggtaaaaa
     6481 agttcgtatc gctaaaaaat ctggcgaaga aattaaatct aataattaat aacaagttga
     6541 aaggaggatc ccactttgaa ccgtttaaaa gaaaagttta acactgaagt tactgaaaac
     6601 ttaatgaaaa aattcaatta tagttcagta atggaagtac caaaaataga taaaatcgtt
     6661 gtgaacatgg gtgtaggtga cgcagtacaa aattctaaag tattagacaa tgctgttgaa
     6721 gaattagaat tgatcactgg tcaaaaacca ttagtaacta aagctaaaaa atcaatcgcg
     6781 actttccgtt tacgtgaagg tatgccaatc ggtgcgaaag taacacttcg cggtgaaaga
     6841 atgtatgaat tcttagacaa attaatttca gtatcattac cacgtgtacg tgacttccaa
     6901 ggtgtttcta aaaaagcatt tgacggacgc ggtaactaca ctttaggtgt taaagaacaa
     6961 ttaattttcc cagaaatcga ctatgataaa gtaagtaaag ttagaggaat ggatattgtt
     7021 atcgtaacga ctgctaacac tgatgaagaa gctcgtgaat tgttagctaa cttcggtatg
     7081 ccattccgta aataattatt taaaggaggc taattaagtg gctaaaactt caatggttgc
     7141 taagcaacaa aaaaaacaaa aatatgcagt tcgtgaatac actcgttgtg aacgttgtgg
     7201 ccgtccacat tctgtatatc gtaaatttaa attatgccgt atttgtttcc gtgaattagc
     7261 ttacaaaggc caaatccctg gcgttcgtaa agctagctgg taataaaaaa gagtctgaaa
     7321 ggaggcaaca atcaatgaca atgacagatc caatcgcaga tatgcttact cgtgtaagaa
     7381 acgcaaacat ggtgcgtcac gagaagttag aattacctgc atcaaatatt aaaaaagaaa
     7441 ttgctgaaat cttaaagagt gaaggtttca ttaaaaatgt tgaatacgta gaagatgata
     7501 aacaaggtgt acttcgttta ttcttaaaat atggtcaaaa cgatgagcgt gttatcacag
     7561 gattaaaacg tatttcaaaa ccaggtttac gtgtttatgc aaaagctagc gaaatgccta
     7621 aagtattaaa tggtttaggt attgcattag tatcaacttc tgaaggtgta atcactgaca
     7681 aagaagcaag aaaacgtaat gttggcggag aaattatcgc atacgtttgg taataaaaaa
     7741 taaggaggtg ccataacatg agtcgtgttg gtaagaaaat tattgacatc cctagtgacg
     7801 taacagtaac ttttgatgga aatcatgtaa ctgttaaagg tcctaaaggt gaattatcaa
     7861 gaactttaaa tgaaagaatg acattcaaac aagaagaaaa cacaattgaa gttgtaagac
     7921 catctgattc taaagaagat agaacaaacc atggtacaac tcgtgcttta ttaaataata
     7981 tggtacaagg tgtttctcaa ggatacgtaa aagtacttga acttgttggt gtaggttacc
     8041 gtgctcaaat gcaaggtaaa gacttaatcc ttaacgttgg ttattctcac ccagtagaaa
     8101 ttaaagctga agaaaacatt actttctcag ttgagaaaaa cacagtcgtt aaagttgaag
     8161 gtatttcaaa agaacaagtt ggagcattag catctaacat ccgttcagta agacctccag
     8221 agccttacaa aggtaaaggt attcgttacc aaggtgaata cgttcgccgt aaagaaggta
     8281 aaactggtaa ataatagata aactctaaag aaaggagtat tgaaatgatc agtaaaattg
     8341 ataaaaataa agtgcgttta aaaagacatg ctcgtgttcg tactaactta tcaggtacag
     8401 ctgaaaagcc acgtttaaac gtatatcgtt caaacaagca tatctacgct caaattattg
     8461 atgataataa aggcgtaaca ttagctcaag cttcttcaaa agacagcgac attgctacta
     8521 cagcaactaa agttgaatta gcaactaaag tcggtgaagc aattgctaaa aaagctgctg
     8581 acaaaggcat taaagaaatc gtatttgacc gtggaggata tttatatcac ggacgtgtta
     8641 aagcattagc tgaagcagca agagaaagcg gattagaatt ttaatttaaa ggagggacaa
     8701 atacatggct cgtagagaag aagagacgaa agaatttgaa gaacgcgttg ttacaatcaa
     8761 ccgtgtagca aaagttgtaa aaggtggtcg tcgtttccgt ttcactgcat tagttgtagt
     8821 tggagacaaa aatggtcgtg taggtttcgg tactggtaaa gctcaagagg taccagaagc
     8881 aatcaaaaaa gctgttgaag cagctaaaaa agatttagta gttgttccac gtgttgaagg
     8941 tacaactcca cacacaatta ctggccgtta cggttcagga agcgtattta tgaaaccggc
     9001 tgcacctggt acaggagtta tcgctggtgg tcctgttcgt gccgtacttg aattagcagg
     9061 tatcactgat atcttaagta aatcattagg atcaaacaca ccaatcaaca tggttcgtgc
     9121 tacaatcgat ggtttacaaa accttaaaaa tgctgaagat gttgcgaaat tacgtggcaa
     9181 aacagtagaa gaattataca attaaggagg gaaaactagt tatggctaaa ttacaaatta
     9241 ccctcactcg tagtgttatt ggtcgtcctg aaacacaacg taaaactgtt gaagctttag
     9301 gtcttaaaaa gactaacagt tcagtagttg ttgaagataa ccctgctatt cgtgggcaaa
     9361 tcaacaaagt taagcactta gtaacagtag aagaaaaata atcaaggagg tgccgaaatg
     9421 aaattacatg agttaaaacc ggcagaaggt tcacgtaaag aacgcaatcg tgttggacgt
     9481 ggtgttgcga caggtaacgg taaaacaagt ggtcgcggac acaaaggtca aaaagctcgt
     9541 tcaggcggtg gtgtaagacc aggattcgaa ggtggtcaat taccattatt ccgtcgttta
     9601 ccaaaacgtg gttttactaa cataaatcgt aaagaatatg ctattgttaa cttagaccaa
     9661 cttaataaat ttgaagatgg tactgaagta actccagctt tattagtaga atctggtgtt
     9721 gttaagaatg aaaaatctgg tatcaaaata ctaggtaatg gttcacttga taagaaattg
     9781 acagtgaaag ctcataaatt ctcagcttca gcagcagaag ctattgatgc taaaggtgga
     9841 gcacacgagg tgatctaatg attcaaaccc ttgtgaactt ctttagaaca aaagaggttc
     9901 gtaacaagat tttcttcaca ctagcaatgt tagtaatttt taaaataggg acttatatac
     9961 cagctccagg agtaaatcct gcagcttttg ataatcccca aggttctcaa ggtgccactg
    10021 agttattaaa tacttttggt ggcggagcct tgaaacgatt ttctattttt gcaatgggta
    10081 ttgtacccta catcactgca tcaatcgtaa tgcaattatt acaaatggat attgtcccta
    10141 aattctcaga atgggcaaaa caaggtgaag taggtagaag aaagttaaat aacgttactc
    10201 gttatttagc aatttcttta gcatttatcc aatctatagg tatggcattc caatttaata
    10261 attatctcaa aggtgcgctg attatcaatc agtcaattat gagttattta ttaatagcac
    10321 tagttttgac agcaggaact gctttcttaa tatggcttgg tgatcaaatc actcagttcg
    10381 gtgttggtaa tggtatttct attatcatat ttgcgggtat tttatcaacg ttgccagcca
    10441 gtctaattca atttggacaa actgcgttcg ttgggcaaga agatacatca ttagcatggt
    10501 tgaaagtatt aggactttta gtgtcactga tactattaac tgtaggtgct atttatgtac
    10561 ttgaagccgt tcgtaaaatt ccgattcaat atgctaagaa acaaactgct caaagattag
    10621 gttcacaggc aacttatcta cctttaaaag tgaactcagc cggtgttatt cctgtaatct
    10681 ttgcaatggc gttcttctta ctgcctagaa cattaacgtt gttctatcca gataaagaat
    10741 gggcacaaaa cattgcgaat gctgcaaatc cttcaagtaa tgttggtatg gttgtatata
    10801 ttgtactaat tatactattc acatacttct atgcatttgt acaagttaat ccggaaaaaa
    10861 tggctgataa ccttaagaaa caaggtagct atgttccagg tattagacct ggtgaacaaa
    10921 ccaaaaaata tattactaaa gttttatatc gtttaacttt tgtaggttct atcttcttag
    10981 ccgttatttc aattctaccg atattagcaa ctaagtttat gggattacca caatcaattc
    11041 agattggtgg tacaagttta cttatcgtta ttggtgtagc gattgaaaca atgaaatctt
    11101 tagaagcaca agtatctcaa aaagaatata aaggctttgg tggtagataa ttttaggagg
    11161 gcacttatga atatcatttt gatgggttta cctggcgcag gtaaaggaac tcaagcaagt
    11221 gaaattgtca agaaattccc aataccccac atttcaactg gtgacatgtt cagaaaagct
    11281 ataaaagaag aaactgaatt aggtaaagaa gctaagtctt atatggaccg tggcgaatta
    11341 gttcctgatg aagtgactgt aggtatcgtt aaggaaagaa tttctgaaga cgatgcaaaa
    11401 aaaggctttt tattagatgg cttcccaaga acaatcgagc aagctgaggc attaaataat
    11461 attatgtctg agcttgacag aaacattgat gctgtcatca atatcgaagt tccggaagaa
    11521 gaattaatga accgtcttac aggtcgtcga atctgtgagt catgtggtac aacgtatcat
    11581 cttgtattta atcctccgaa ggtcgaaggt atttgtgata tcgatggtgg taaattgtat
    11641 caacgagaag atgataatcc tgaaacggta gctaatcgtt tgagtgttaa tattaaacaa
    11701 tctaaaccta ttttagattt ctatgatcaa aaaggtgtat tgaaaaatat tgatggttca
    11761 aaagatatta gcgacgttac caaagatgtc attgatattt tagatcattt gtaatagatc
    11821 aacttttagc ggtctatttt aagtgaggca tgatttaatc atattagata gatgagagtg
    11881 gcaattaacg ataattaatt cccgtaattt ttgtttaact tataaaatta atcactgata
    11941 gattcgtaac aataaagttg tctattgacg attaccttac tattgcaaaa agggggaagt
    12001 taatcaatgg ctaaacaaga tgtaattgaa ttagaaggta ctgtattaga tactttaccg
    12061 aacgcaatgt ttaaagtaga attagaaaat ggtcatgaga ttttagctca cgtaagtggt
    12121 aaaatcagaa tgaattacat tcgtattcta cctggcgaca aagtaactgt tgagatgtct
    12181 ccgtacgatt taacacgcgg aagaattact tatcgttata aataatcgtc actccataat
    12241 atagggaggt ataaaaatga aagtaagacc atcagtaaaa cctatttgcg aaaaatgtaa
    12301 agtcattaaa cgtaaaggta aagtaatggt aatttgtgaa aatccaaaac acaaacaaag
    12361 acaaggttaa taaaagagag gtgtaaatta atatggcacg tattgcagga gtagatattc
    12421 cacgtgaaaa acgcgtagtt atctcattaa cttatatata cggtatcggt acgtcaactg
    12481 ctcaaaaaat tcttgaagaa gctaacgtat cagctgatac tcgtgtgaaa gatttaactg
    12541 atgacgaatt aggtcgcatc cgtgaagttg tagacggtta taaagtcgaa ggtgacttac
    12601 gtcgtgaaac taacttaaat atcaaacgtt taatggaaat ttcatcatac cgtggtatcc
    12661 gtcaccgtcg tggtttacca gttcgtggtc aaaaaacgaa aaacaacgcg cgtactcgta
    12721 aaggaccagt taaaacggta gctaacaaga aaaaataata ggtaaaggag gcaaatttta
    12781 aatggcacgt aaacaagtat ctcgtaaacg tagagtgaaa aagaatattg aaaatggtgt
    12841 agcacacatc cgttcaacat tcaacaacac tattgtaact atcactgatg agttcggtaa
    12901 tgctttatca tggtcatcag ctggtgcatt aggattcaaa ggatctaaaa aatcaacacc
    12961 atttgcagca caaatggctt ctgaaactgc atctaaatca gctatggagc atggtttaaa
    13021 aacagttgaa gtaacagtta aaggacctgg tccaggtcgt gaatcagcta ttcgtgcatt
    13081 acaatctgca ggtttagaag taactgcgat cagagacgtt actccagtac ctcataacgg
    13141 ttgtcgtcca ccaaaacgtc gtcgtgtata atttatgatg gtattgttac aggtcactga
    13201 gcaaacattt taaattaagt cgacgtatat aaggaggata tttaaatgat agaaatcgaa
    13261 aaacctagaa ttgagacaat tgaaattagt gaagatgcta aattcggtaa gttcgttgtt
    13321 gaaccactag aacgtggcta cggtactaca ctaggaaact ccttacgtcg tatcctacta
    13381 tcttcattac caggtgcagc cgttaagtac attgaaattg agggagtttt acatgaattc
    13441 tcagcagtag acaatgtagt tgaagatgtt tctacaatta ttatgaacat taaacaatta
    13501 gcattgaaaa tttactctga agaagataaa actttagaaa ttgatgtacg tgatgaaggc
    13561 gaagtaacag caagcgacat tacacatgac agtgatgttg aaattttaaa cccagagctt
    13621 aaaattgcaa cagtatctaa aggtggtcac ttaaaaattc gtctagttgc taacaagggt
    13681 agaggttacg cattagcaga acaaaataat actagtgatt taccaattgg tgtaatccct
    13741 gttgattcat tgtattcacc tgttgaacgt gtgaactata ctgttgaaaa tacacgtgta
    13801 ggtcaaagca gtgattttga taaattaaca ttggatgttt ggactaatgg ttcaatcaca
    13861 ccacaagaat cagtttcatt agcagcaaaa ataatgactg aacacttgaa tatcttcgtt
    13921 ggtcttactg atgaagcgca aaacgctgaa atcatgattg aaaaagaaga agatcaaaaa
    13981 gaaaaagtat tagaaatgtc tattgaagaa ttagacttat ctgtacgttc atataactgc
    14041 ttaaaacgcg caggaatcaa ttctgttcaa gagttagctg acaaatctga agctgacatg
    14101 atgaaagtgc gtaatttagg tcgtaaatct ttagaagaag ttaaatacaa attagaagat
    14161 ttaggattag gattaagaaa agaagattga taaaggaggt taactcatgg gttacagaaa
    14221 attaggtcgt acttctgatc aacgtaaagc tatgttacgt gacttagcta catcacttat
    14281 tattagtgaa cgtattgaaa ctacagaagc tcgtgcaaaa gaagttcgca gtgttgttga
    14341 gaaattaatc actttaggta aaaaaggaga tttagcttct cgtcgtaatg cagctaaaac
    14401 tttacgtaat gttgaaatct taaacgaaga tgaaactaca caaactgcac ttcaaaaatt
    14461 atttggtgaa atcgcagagc gttacacaga acgtcaaggt ggttacactc gtatccttaa
    14521 acaaggccct cgtcgcggtg acggtgctga atcagtaatt atcgaattag tataagaatt
    14581 tatataaata cactaactac ttttatataa agcaaatgag tgcaacgata atgtttgcca
    14641 catacaaata ttgtctagct cagagtaccc catcaactaa ataaagattt aaagcgtgaa
    14701 ctcaacaaac ttatgatggg gtgcgcgctt ttttattgaa taagatatcg gacttatgaa
    14761 cagaaatgtg aactgcaccc agtctagtag acaattaaat aattaaaatt aatggttggt
    14821 tttgttgtta tacagaatca gccattttta atgtaattct ttcatgatta aagaaaagaa
    14881 taaaatcatg aattgttttt gtagcttctt ctacactatt aaatttaaag tgcttatttc
    14941 ctctaaaaat ttcacttttt attgtacccc atacaccttc cattggaccg ttatcgatac
    15001 atttgctaac acgtgacata ctttgaatta tgccacattc atccaacata tgtttaaatg
    15061 ttttcgatgt atattgaaaa cctcggtcac tatgaaataa aatacctttt gtattttcta
    15121 ctttttctat cgcttgattg aatgttttaa atactaattg attattgtta gaagggccta
    15181 gctcataact aacaatacgt ttagcaccta agtcataaat ggcactcaaa tatatcttac
    15241 taccttcttt gattttaaat tctgttacat ctgttaacca aactttattt ggcgtatcaa
    15301 tgtcaaattg tcgattaagc ttattttctg atgtgatttg tggcgttgaa cgtcgataag
    15361 gttttcgctt ttgacgtatt actgctttta aattaagttc tttcatcaaa cgataaattc
    15421 gctttcggtt tacttgttta cctagtttta agcgaatata gatataaatg cgacgatagc
    15481 cataaatacc attatactta tgatagattg attctatttc tcgcttaagc tgttcattct
    15541 ctaattcaag ttcactcgat tgataatgta cccacttata ataactcgct ctagaaatgc
    15601 ctaaaatacg acacaagcgt tggatagggt attgatgttt taacgcttcg atcgttttgt
    15661 atgccgtgat ttgttttctc tttgaatcat ctctctttcc aatgcttgaa acttttttaa
    15721 aacgtcattt tccatctcta ataatcgatt ttgtgctttt aatgcttgaa tttcagcttc
    15781 tttttgttct tctggcgtca tcatagattt gggttttcct ttgcctctgc catctactaa
    15841 tccattttta ccatgtgctt gatacttatg aacccactgg taaatttgac cgtatgagat
    15901 attaaagtgt tctgcaactt ctctataaga ttgtttatga ttcaagtaat attcaacaat
    15961 tgttacacgt tcttcaaatg tggtttttct tgctttcatg atatacaccc cagttttagg
    16021 agattgattc ttaatctctt ttcctacagt atacattttt atccaatttt tgacttgtgt
    16081 cgaactacga atattgtatt tgattgcgat gtcttgggtt gattgatttg aattcaaata
    16141 ttcatttaca acttttaatt ttagttcctt tgaatagtga ttgtattgtt tttgtgtaag
    16201 tagtgtttca ataccatggg ttttataata tttatactta ggatacaaag tattgacatt
    16261 aatatctaat tgtaatgtat cgattaagta ttgaaacgac acgccttttt catattcttg
    16321 aaacatatat agcagtacat ctatgtctac tttaggtttt attgacataa ataaagctcc
    16381 cttcaaagtt ttcatttttt caatgtctac tttgaaggga gcatttcaat gtttttaagt
    16441 ccgatttctt tatatctact tttttgagtg cgaatctaaa aatatgatca agctagtttg
    16501 atcaatttca ctagaactga atgaagtgaa agtattatcg ttgaatcaaa cgcggccaac
    16561 actagtaaat catttatttt attaacgttt atacataatt gttagagagg aataaatgtc
    16621 cttatcatta gttgttaaac aacactttaa agactagaaa tgagtcagaa aatactagta
    16681 atatatagca aacccagtca atgtcatata cagcgattta taaatattgt aaaatatgat
    16741 agatacacga atcgtaaagg agagagacaa gtggaggata agaattcagt tattgtattt
    16801 aaaaatgttt catttcaata tcaaagtgat gcatccttca cattgaaaga tgtttctttt
    16861 aatataccta aaggtcagtg gacatctatt gttggtcata acggttctgg gaaatctaca
    16921 attgccaagt taatgattgg catagagaaa gttaaatctg gagaaatttt ttataataat
    16981 caagctataa cagatgataa ttttgaaaag ttaagaaaag acataggaat tgtatttcag
    17041 aatccggata atcaatttgt tggttcaatt gtaaaatacg atgtggcatt tggactcgaa
    17101 aatcatgcgg ttccacatga cgaaatgcac agaagagtca gcgaagcact tgaacaagta
    17161 gatatgttag aacgtgcaga ttatgaacct aatgcattat cggggggaca gaagcagcgt
    17221 gtggctatag caagtgtatt agcacttaac ccctctgtca ttatattaga tgaggcgact
    17281 tctatgttag atcctgatgc acgtcaaaat ttattggatt tagtgagaaa agttaaatca
    17341 gaacataata ttacaatcat ttctattacg catgatttat ctgaggcgat ggaagcagat
    17401 catgttatcg ttatgaataa agggactgtc tataaagaag gaacagcgac tgaaattttc
    17461 gaccatgcag aagagttaac aacaataggt ctagatttgc cattcccaat caaaataaat
    17521 caaatgctgg gacaccaaac atcattctta acttatgaag ggctggtgga tcaactatga
    17581 ttatacgctt tgacaatgta agttatacct atcaaaaagg gacaccatac cagcatcaag
    17641 ctattcatga tgttaataca gaatttgaac aaggtaaata ttatgccatc gttggacaaa
    17701 cgggtagtgg taaatcgaca ttgatacaaa atattaatgc actgttaaag ccaactactg
    17761 ggacagttac agttgatgat attactatta cacataagac caaagataaa tatattagac
    17821 ctgtaagaaa aagaattgga atggtatttc aatttcccga atctcaatta tttgaggaca
    17881 cagtagagcg tgaaatgata tttggaccta aaaactttaa aatgaattta gatgaagcca
    17941 aaaactatgc ccatcgtctg ttgatggatt taggcttttc aagagatgta atgtcacaat
    18001 caccatttca aatgtcaggt ggacaaatgc gtaaaatagc gattgtatcg atattggcaa
    18061 tgaatcctga tattatcgtg gttgatgaac ctacagcagg acttgatcca caaagtaaac
    18121 gacaagtaat gcgattactc aagtcactac aaacagatga aaataagaca attatcctaa
    18181 tttcacatga tatgaatgaa gtcgcacgtt atgcagatga agtcatcgta atgaaagaag
    18241 gtagtatcgt gtcgcaaaca tcaccaaaag agctcttcaa agataaagaa aaattggcgg
    18301 attggcatat tggtttgcct gaaattgttc agttacaata tgactttgaa caaaaacatc
    18361 aaacaaaatt aaaagatatt gccttaactg aagaagcatt tgtaagcttg tataaggagt
    18421 ggcaacatga aaaataaatt gattataggg cgatatttac caattaattc ttttgttcat
    18481 catcttgatc caagagcaaa gttaatgttt gtttttcttt ttattatatt aatatttttc
    18541 tgtcattcac cattaacata tctttggatg tttgcactta tcttattctt tatgagatta
    18601 gcaaaaattc aattatggtt cctaatcaaa ggtttaacgc caatattttt ctttttaatc
    18661 ttcacattaa tgatgcatat atttttaact aaaggtggat atgtattagt tgaatggcat
    18721 ggcatcacga ttgaaactaa tggtatttta gaagggctat atatatcact gcgtttaatt
    18781 gggattgtga tgattgcaac aataatgaca ctatctacaa gtccaattga tttaacagat
    18841 gcgtttgaaa gattacttgc gccactaaag atgtttaaat taccagttca tcaattaagt
    18901 atgataatgt ccattgcgtt acgattcatc ccgacgttaa tggatgagtt agataaaatc
    18961 attttggcgc aaaagtcgcg tggttctgaa ataagttcag ggaacattgc aacacgtatt
    19021 aaatcattta ttccattact agtgccacta ttcatctcag ctttccaacg cgccgaagaa
    19081 ttagcggtcg caatggaagt taggggttat gatgccaatg ttaagagaac gagctacaga
    19141 cagcttaaat ggcaattgag agatacgtta tctttaatca tgattatccc aattgcaatt
    19201 attttattcg tattaaaata ttcaggagtg taaattcatg cgtatattag tagaaattgc
    19261 gtatcaagga aataattttc taggttttca aattcaacaa aatggtcgaa cggtacagca
    19321 acaatttgaa aagctattac aacgcattca taaaagacat gtgagaatcc atccttcaag
    19381 taggactgat agaggtgtac atgcgataca gcagtacttt catttcgaca cagatttgaa
    19441 tatacctatg tcacaatggc aatatgcaat gaaccgaacg ttacctgatg atatttatgt
    19501 caacaatgta gttacagtcg atgacgattt tcattgtcgt tacgattgtg tcggtaaacg
    19561 ctatcgatat aaagtatatc aagcacaaca acgcgatcca tttcaaagtg gtttgaaaac
    19621 atttattcct gaaccattgg atttagacaa aatgaacaga gcggcacaac aattcatagg
    19681 tacacatgat tttacgggtt tttgttcgca aaaaactgaa gtagaaagca aggttagaac
    19741 attataccaa agtgaaattg taaagacaga tgatggcttt gattatattg tcactggatc
    19801 tggattttta tataacatgg tgcgtgtctt agttgcattt ttaatagaag tcggaaaagg
    19861 gcgacatgaa gtatcagatg taccaaaact tcttgaatca aagaatcgta agaatgtacc
    19921 tttcactgca ccggctgaag gattatattt agagaaaatt tatttagatg aaaatgagtt
    19981 attaaaagac tttggtaacg atatcaaaat acatcgtaaa aaatcattat aaaatgactg
    20041 attgccattg acaaaaagct tgtgaaatta taagattatt aacggtattg ttttatatcc
    20101 accccacgat aagccccgga aacttattgt gttacaagat atataagcag aaacgaacaa
    20161 cagttaacaa aataaatgaa atgaaacgtt ttaaaaatga aacaaatgaa atcatctatt
    20221 aggttatgaa actgtttata gcttgaatag aagcatttat tttttaggag gacaattatt
    20281 atgcgtcaaa catttatggc aaatgaatca aacattgagc gcaaatggta tgttatcgat
    20341 gctgaaggcc aaacattagg tcgtttatca tcagaagtag catctatctt acgcggtaaa
    20401 aataaagtaa cttacacacc acacgttgat actggtgatt atgtaatcgt tattaatgca
    20461 tcaaaaatcg aatttactgg taacaaagaa actgacaaag tttactaccg tcactcaaat
    20521 cacccaggtg gtatcaaatc aatcactgct ggtgaattaa gaagaactaa cccagaacgt
    20581 ttaattgaaa attcaattaa aggtatgtta ccaagcactc gtttaggcga aaaacaaggt
    20641 aaaaaattat ttgtatatgg tggcgctgaa catccacacg ctgcacaaca accagaaaac
    20701 tacgaattac gtggttaatt agaaggagga aatgactttg gcacaagttg aatatagagg
    20761 cacaggccgt cgtaaaaact cagtagcacg tgtacgttta gtaccaggtg aaggtaacat
    20821 cacagttaat aaccgtgacg tacgcgaata cttaccattc gaatcattaa ttttagactt
    20881 aaaccaacca tttgatgtaa ctgaaactaa aggtaactat gatgttttag ttaacgtaca
    20941 tggtggtggt ttcactggac aagctcaagc tatccgtcac ggaatcgctc gtgcattatt
    21001 agaagcagat cctgaataca gaggttcttt aaaacgcgct ggattactta ctcgtgaccc
    21061 acgtatgaaa gaacgtaaaa aaccaggtct taaagcagct cgtcgttcac ctcaattctc
    21121 aaaacgttaa gctgcagctg ttgcgaactt gttcgcttac agatggagtt cgcaaattgc
    21181 gtcagcaatt tgtgttcaac tgaagaaacg tttacagcac tctttggatt attccaaaga
    21241 gtgttttttg tgtttttata gagtcattta gatactattt agttacccct agaggaacta
    21301 atttgtttca attctgctta aaattcaatg atatgaaact ttaggaaacc atatgaaatg
    21361 acctggtttt tctttgggat tcgtacgaca agcagataga ggttgtaatg atattcgata
    21421 gatatagtat tgaatgttat aataaagtca ataagatgcg agattgcgag gtttatatat
    21481 gactagtagt aagtatattg aatatttgca aactgaggtt gaaggacagc ttctagatcg
    21541 aaagagggca tctataaatc ctaaagatgt tgctcaacac attagtgcgt ttgcaaatgc
    21601 tgaaggtggc aaactggtaa ttggtattga agataatgga gaaatttcgg gatttaatca
    21661 ttcaaaagca aaaaagaaag aggtttatat tgaagcacct ttcgaacatt tatatagaat
    21721 acctaaatat acatatgaaa taatagaagt gaagaaacgc gatggaacta tggatgaaat
    21781 tcttattttc gacattgaac ccagttatga tgccataatc accttaaaag atgattcagt
    21841 atatttaagg attaacgata aaagtagaaa actgacacac aatcaaatta caaatctaga
    21901 atacgaccgt ggttcacgaa gattcgaaga agaattagta gagtattcta gtattgaaga
    21961 tgtgaatgaa gaactagttg atgaatttaa gcaattactt aatacaaacg tagataatga
    22021 aaaattatta aaagctagag gatttatgcg tgaaggtaaa ttgacagttg caggtttgtt
    22081 attgtttagt aatcacatca atgtttattt accaggcgct agaattcgat ttatgaggta
    22141 tgaaggaaca aaagaagaat ctgggacaag acttaacatt gtaaaagata ttacttttga
    22201 taaagctttg ccagtagcaa taagagaagc gagagccttt atcaatacac aattacgtga
    22261 atatactttt ttaggtaaag agggaagatt tgtaactttg ccagaatatc cagaatttgc
    22321 ttggtttgag gggatgataa attctattgt acatcgcaga tatgataatc aaggcgatca
    22381 tataagaata aaaatgtttg acgacagatt agaaattagt agcccgggtg cactgcctgc
    22441 ttctgttacg ttagagaata ttaaagaaga aagatattca aggaatccaa aattagctgc
    22501 agcattaact caatataaat gggtgagaga atcaaatgag ggcgtgggac gtatatttga
    22561 cgaaatgaag caatattttt tagatgatcc tgtatactca gagcctaata atagttcagt
    22621 acaactagct ttgaagaata acataatagc aaggaaaaaa agagaaacag ggagagtgag
    22681 taatataata acacctgaat tatttgaaag tttaaatgag caacaagaat taattgtgag
    22741 acatttatat aatcaaggcg aactaacagc atctaaaatt gctaatatta ttcaaaggtc
    22801 tgttcctact gctagaaaaa gattgaaaga attagaggaa atgaatatta tatcgacaca
    22861 tggatcaagt aagaatgatc caaagagaac atattattta ctcggattcg agtaaagaga
    22921 aaaattttcg agtaactcaa aagaaaaata ttaaaaaatc atgagaaacc aatgatatca
    22981 agggttttgt gagctttcga gtaaagagga aaattttcga gtaactcaaa ataaaaacat
    23041 taaaaaatca tgagaaacca gtgatatcaa gagttttgtg agctttcgag taaagaggcg
    23101 gattttcgag tagtactatt taataagaat tacaagttaa ataaaatacc tactaatcag
    23161 aagttgacag agattgtaga atttctgtca gatggtaagt tagcaagtag gctagaaatt
    23221 caagaaacaa tgggacttca aaaaagttat acatctgagt taattgctga actaaagaaa
    23281 ttagagatta tcggatctga aggtagaggt ccgggaacta gatattattt aattagagac
    23341 taataaattc atcaagtgat aaccggacga taacctattg atatacataa aaacattgat
    23401 aaatcaatat atttacgacc ggacgataac cggatgatac gagagtatat accaccggac
    23461 agcagtttat ttagttaccc tttagttacc cctaggtgat atttgatgca attctatgtg
    23521 aacgaaaaaa ttaggaatcc tttagtatca aggcttacga cagtctatac aattgtacga
    23581 aaccctaaag ttttttgaac gtaaaaaacc aagtcttaaa gcagctcgtc gttcacctca
    23641 attctcaaaa cgttaattgt cggacgatat atacaaaaca cctcgatatt atgtcgaggt
    23701 gtttttttac gtttgtgtgg ggaatatgga atgtgtagag tataatgaat tttcctttat
    23761 ttaatgtcta aggaatagaa taaattcaac tatgaattat tgatttacac gtataatttg
    23821 aacatgctta cacaatcaaa agtgtacatt attaaattgt catttagctt gcggaggacg
    23881 gacaataatg ttttaatcat agtggttcaa gggaaagggc tatcggatga aaatcggaag
    23941 atagctcaag aaccatgatt taagagtgtt atcaaaataa ggagtgactt aattgataga
    24001 ttctgtattt tggaagaaag cttttattcc tgtttatttt attgttgcaa tgctggtgtt
    24061 tctacttttt aggttttata ttaaaacaaa taacttttct atatacttaa tgattatctt
    24121 cttaatgtgt ttaggaactg catctatcat ttataactat aaaaacaatc gataaatata
    24181 agtatgaaat gtataagtca cagttagatc taagtcttgc tgtggctttt tttatcgaaa
    24241 tgacaatttt aaatcgccaa aaacagcatt ttcaaaccgt cataaaacag cattttcagc
    24301 ccgccataaa acgacaattt caaaccgtca ttgactaaag acctcattct caaatatgct
    24361 aacaatcctc cacaccaatc aatccaacat cccttataat cactcccttc aaaatctact
    24421 catgcatttt tggaatactt agtattacaa ataacgattt ttattcatct tacaaaggat
    24481 atataatgta ctgaaggcaa tttttatgta tcacaaatct aattgtatat gtaaagtttt
    24541 gataaatatc attaatttta cataactatc attagattac aaatcacaat gtaattacat
    24601 gtaatacaca tctacacatc acatttgaag ggaaatgaat ataaatgact gataaaaagt
    24661 acactgcagc cgatatggtt attgatactt tgaaaaataa tggggtagaa tatgtttttg
    24721 gtattccggg tgcaaagata gactatctat ttaatgcttt aattgatgat ggtcctgaac
    24781 ttattgtcac tcgtcatgaa caaaacgccg caatgatggc gcaaggtatt ggaagattaa
    24841 caggcaaacc gggtgtagta cttgttacaa gtggtcctgg tgtaagtaat ttaacgactg
    24901 gactattaac agctacatct gaaggggatc ctgtcttggc gttaggcggc caagtgaaac
    24961 gtaatgattt attacgatta acgcatcaaa gtattgataa tgctgcgcta ttaaaatact
    25021 catcaaaata cagtgaagaa gtacaagatc ctgaatcatt atcagaagtt atgacaaatg
    25081 caattcgtat tgctacttca gggaaaaatg gcgcaagttt tattagtatt ccgcaagacg
    25141 tgatttcttc accagttgaa tctaaagcta tatcactttg ccaaaaacca aatttaggag
    25201 taccgagtga acaagatatt aatgatgtca ttgaagcgat taaaaatgca tcatttcctg
    25261 ttttattagc tggtatgaga agttctagtg ctgacgagac gaatgccatt cgcaagctag
    25321 tagagcgtac gaatttacca gttgtagaaa cattccaagg tgcaggtgta attagtcgtg
    25381 aattagaaaa tcatttcttc ggtcgtgtgg gcttattccg taatcaagtt ggtgatgaat
    25441 tattacgtaa aagtgattta gttgttacaa tcggttatga tccaattgaa tacgaagcta
    25501 gtaactggaa taaagaatta gaaacacaaa ttatcaatat tgacgaagtt caagctgaaa
    25561 ttactaatta tatgcaaccg aaaaaagagt tgattggtaa tattgctaaa acgattgaaa
    25621 tgatttctga aaaagtagat gagccattta taaatcaaca acatttagac gaattagaac
    25681 aattaagaac acatattgat gaagaaactg gtattaaagc gacgcatgaa gaaggaattc
    25741 tacatccagt tgaaattatt gaatctatgc aaaaggtatt aactgatgat acaacagtga
    25801 cagttgatgt tggaagtcac tatatttgga tggcacgtaa tttcagaagt tacaatccaa
    25861 gacatttatt atttagtaac ggtatgcaaa cacttggtgt agcattaccg tgggcaattt
    25921 cagctgcgct tgtacgccct aatacgcaag ttgtatccgt tgctggcgat ggaggctttt
    25981 tattttcatc acaagattta gaaacagccg tacgtaaaaa tctaaacatt attcaactta
    26041 tttggaacga tggaaaatat aacatggttg aattccaaga agaaatgaaa tataaacgtt
    26101 catcaggtgt cgacttcggt cctgtagatt tcgtaaaata tgctgaatca tttggcgcaa
    26161 aaggtttacg agttactaat caagaagaat tagaagcggc aattaaagag ggctatgaaa
    26221 cagatggtcc agtattaatt gatatacctg taaattacaa agataatatc aaactttcaa
    26281 caaatatgtt acctgacgta tttaactaaa acaaagataa atgttaatga ggagtgggaa
    26341 attttatggc tagtgtttta tatcaacatg gtacattagg cacattaatg gcaggtttat
    26401 taaaaggaac tgcatcaata aatgaattat tgcaacatgg tgatttaggt atcgctacac
    26461 taacaggttc aaatggtgag gtcatctttt tagatggaaa agcctatcat gcaaatgaac
    26521 ataaagaatt tgtggaatta aaaggtgatg agttaacacc atatgcaact gtaactaaat
    26581 tcgtagcaga tacaagctat gaaacgaaag ataaatcttc tgaagcagtt tttgcagaaa
    26641 ttaaggaaaa gatgttgagt gaaaatttat tttctgcagt aaaaatttca ggcttattta
    26701 aaaaaatgca tgtacgtatg atgccggcac aagagccacc ttatacacgt ttaattgact
    26761 cagctagaag acaacctgaa caaactgaaa cgtacgtcaa aggcgcagtc gttgggttct
    26821 ttacaccaga actattccat ggtatcgggt cagcaggatt ccatgtacac tttgcaaatg
    26881 atgatcgtaa ctttggtgga catgtcttag attttgaagt agaagatgtt aaggtagaaa
    26941 tccaaaatat agaaacattt gaacagcatt ttccaattca agatgaagat ttcataaatg
    27001 caaatattga ctataaagat attgcagacg aaattagaga agctgaataa tgaatgcgga
    27061 aatgtaatga tggattataa aaattgaagc attgacttca taatgttcga tttggaaatt
    27121 tagcaatgat aaaaggttgt gtaatacggt taagcatggt tgtcatagag catacgtcta
    27181 tgtaataaaa taaatccctt cactattaac ggtagtgagg ggatttattt atgttctagc
    27241 tatttgtaat ttgctgttat gttagattat aaattacgga agacgttaat atagaattta
    27301 ctttagtata ggccaatcta atctattgtc agtagaagca atcaataata aattacaagt
    27361 tcgattattc atttcatact aaaaagacaa acgcaatttt tcagcgtttg ccctgtataa
    27421 ctattttcat tttcaaatta ttatttaact acaatttgta tttgtttgat tgtacttgga
    27481 tcaattgaat ttttagcgtc gtcatttttc tttagattca ctactttttt attaccatca
    27541 ttcaaagtaa gtgtatacgt tgctgtttgt gcattattaa ttttttctgt tgtaacacca
    27601 cgttgagaag ctaattgatt ttttacttta ctgtcaattt cttgataaag tactttttta
    27661 ttttctggaa aaacaaagta agttcgatgt aatgcagtaa taccatctac tgaaatcgtg
    27721 taaggaacag tgtgataacc atccattgtc atttctttat agccgttatt actatctgca
    27781 gatgcttcgt gactaggtaa tgcagctccg accgttgata ataagcccaa tgctaaagtg
    27841 gcagtaacaa atgattttag tttcatatct caatctcctt ttaaagttgt ggtatattac
    27901 actatttata taacacgttt cataaagttt aaaacatact ttttgatttt tttatatttt
    27961 tgatttttag taatagtttt taacgaggga tatgacccgt gtattttgaa aagtggtaag
    28021 caatgttttt agtttatagg gattcattag ttgtatataa taagcatgaa aagcatatag
    28081 tcgatcttat acttttaatt gagacttata gtgtatttaa gttatagata aattcattaa
    28141 aatattatgg aggaatgagt atgtcaaagc gtctattatt gtttgatttt gatgaaacat
    28201 attttaaaca taatacaaat gaagaagatt taagtcattt aagagaaatg gaaaaattgt
    28261 tggaaaaatt aactaataat aatgaagtga tcactgttgt attgacagga agtacatttc
    28321 aaagtgtcat ggataaaatg gatcaagtta atatgacgtt taaaccatta catatctttt
    28381 ctgatttgag ttctaaaatg ttcacttgga ataatggcga atatgttgaa tcagaaacat
    28441 ataaaaagaa agtcttgagc gaaccatttt tatttgaaga tattgaggat atattacatc
    28501 atatttctgc gcaatataat gtcgaattta ttccacaaag agcatttgaa ggtaatgaaa
    28561 cacattataa tttttatttt cattcaacag gtaatcacaa caatgatagg cgtattctag
    28621 aagtacttgt cagatacgca aatgaccaaa actatacagc gagatttagt cgaagcaatc
    28681 cattagctgg tgatcctgaa aatgcgtatg atattgactt cacaccgtca aatgctggaa
    28741 aactatatgc tactcaattt ctgatgaaga aatataatat accagtaaaa tcaatattag
    28801 gttttggaga tagcggcaat gacgaagcat atttaagcta tcttgaacat gcctatttga
    28861 tgtcgaatag tagagatgaa gcattaaagc agaaatttag attaacaaaa tatccatatt
    28921 atcaagggat aacactgcat gttaaagaat ttgttgaagg taagtatgat tattgattgg
    28981 ttgagtgata tgtatacttt ttaagtacaa atattaatat ttaagggatg attagaaaat
    29041 atagtgaatc gactacactt tagttttaaa aaattattta tatgttgatt tgtccaatta
    29101 agtgtagacg attcaggtag cgcaaaaatg acgcaggaac tggagaaaga attgatacca
    29161 aaattaatat aagaggttaa gtatatatga ataaaacaaa ctttggggat tatttaaata
    29221 aaagtaagag caggaaaata tataagattt ttgatcaaaa tttgaagcga gagtttagtt
    29281 attggcagtt taggaaactt ttcaaatctt atacatggca gtctgatgta catgaattaa
    29341 aagtgtgtac tcaaaatgat ggtactattg agcaaatatg gatagataaa gcaaatcaat
    29401 ttggagtggc agtgacatgg aaagataata gtattatcgg atttgttatg cgtccgttaa
    29461 tcaattatga tgcgattaag aatgaaacga ctttaaaata cgcagtgccg acaacagatt
    29521 gctatgaggt tttttggggt ggagataatg aattatataa ttatcactat ttatacccac
    29581 atcaaaggta tgctattgat ttagttatta aagaaaatca taaaacatat aagaacctag
    29641 gaaaagtaaa tgctgattat ttttgttttg gaaaagatat cattgcgcca gcaaatggta
    29701 cagttgaaaa agtagtgaat ggggttcaag acaacagtat tggaagtacg aaggaatcac
    29761 agtttttagg aaattatatt gtaatcaagc acgcagaaaa tgagtatagc ttaatagctc
    29821 atttacaaca atattcaatc attgtgaatg aggggcaaaa tgttaaatat ggtgacatca
    29881 ttgggaaggt tgggaattct ggcaattcta ctgaacctca tatacatttt caagtaatga
    29941 atgataagaa tattgaagca tgtacatctt taaaaattcg atttataaat aatcgagaac
    30001 ttatcaaagg agatgtggtc tgcggattac aaggtgaatg atagttatgc ttataaaatc
    30061 tcgacactat aaaaatggta tagtgccgag attttcttgc ttatttagtt aattcaaagc
    30121 gtacgccgga ttcattagaa gtcgacgtat ttttgtttat aatagagtat ccagtcattg
    30181 aaattttaga ttcaatatct gaaacggaat ttgtagattc gggattatag aagctacatt
    30241 cataagtgtt atcatctttc tttgtaagaa taaacattcc tttggcttta acttcgactt
    30301 tagtgttatt aatttcaaaa gttttagcgc tatcactata attgatacca gcccaaaccg
    30361 attcattatc tttcacaacg tggaagtcat cttcttgctt aacgacagtt actttgcttg
    30421 ctttggattt aaagacatct ttagataagc ctggatacaa cacatatgca tatttactat
    30481 ctgtattaga atgcttttga gttacttcat aatactcatc agttttttgt atatcctttt
    30541 ggcttttatt tatatcactc cacttaccag tatgactttc tttttttaca gttattttcg
    30601 attcgtttaa aaaatgataa cctatattgt tttgagtgct attcgttgac tctaaaaaga
    30661 gggaattggt ttcctgatta tcggaattgg ttgtttgcgt atcatcttta tataatttat
    30721 aatcattcgc tttgcgattt tcaatcgttg taactggatt ctttgatgaa tcagtacttt
    30781 taatgccagt tcctaagaag acaattttat cgtttaatat gaaatatgat tttttggcag
    30841 ttaaagtttt gtcctgattt ttaaaatcca ttccgatact agcatgttgg tcatcgaatt
    30901 ttgttcctcc tacaaaagtt ttactagact ttttaacatc cgtactttta ggttcttcat
    30961 tatctaaagt tgtagtacct gctaaacgtt tcatatcagc tgtcgcccag aagttatcac
    31021 ggtagtgttt cacatcgcta ttgtataaat aagacattcc agcaccagtg tgccaacctt
    31081 ttaagttctc tccgttgata ctttcgtaac gtgcgacgtt tttcgacgtc atacttaaac
    31141 caaatgcaaa gtctaagcct ttattgtgat aggtgacacg atccatgtca ttatatattt
    31201 taagttgttg tgttaaaccg ttagtagaaa tagtgctgtc ttccattaaa gacttcattt
    31261 tgcttatatc cgaataagag cttaaataat cggtttgttt ataacttgaa tctgactcta
    31321 ctgaagtctt gacaatcttt ttatatttag cttttgtaga cttatccatg gtatcactca
    31381 atctcaacaa tgatttcatt actgttgcag atgctgagtg actcgtttca ttttcacgac
    31441 tgatagctct acctcttgat aaatccatca tttcaccttt ataaatgagt ggcataaatc
    31501 cgtcgtcaat ccatgatttt aaggttgtat cattttggtt actatcatta aaaggtgttt
    31561 cttttatcat cggcatcatt tgagaaatac cctctaatag tacaacgcca taagcaccag
    31621 tgtatgggac gtcttgatga tcaatgtaag agccatcttt atagaatcca ttacgttctt
    31681 ttccagtggc agaattttgc gcgtaagtga agactgtatt aaatgaatct atagactttt
    31741 tcatcatatc tttgtcttct tcgataatac tttctaaaag ttttacttta gaaatgtcta
    31801 ctaaatttcc gcctttagca ggttcagatt ttcctacaga agataatatt ttgtcactat
    31861 ctggggcgaa agttttaata ggagctgtat attttttctt ttcttcatca gtgaaatctt
    31921 cttgtaagag tataagcgta tttgttaatg ctctaggtgt tccaatttca taatcccacc
    31981 aatttaagtt ggtatttcta gaagttttat ttttaaagtt tgaggtcaaa tcagcaactt
    32041 ttttatctgg ttcttttcca tatgcatttt tatgcagcca ctcaagtgca tcttttactt
    32101 ttgtcttgtt ttcatctgtt tttaatttag tatttttatg tttcatcgct tctgagattt
    32161 tctcaatatt acgataggta cgagtcatat ccgcagactt cgtatctaaa tcttttgagc
    32221 tttcccacaa gtatttccta tcactttcag ttttcatttc cttgagtaat ttctctgcct
    32281 cattctccgt agcctcaaac ttcttcttca tatctggatt attctcatca tacttatcat
    32341 aaccatagtt aacgtccagc catatgttcc tcaacttttc ataatctgtc gtttgaacat
    32401 tcgtatcagc cacagcgatt tgatgtttat caatacttct gaattcacca ccattcaaag
    32461 taatcacacc agccattaat aacgtaatgg tggacaattt ttgccatttc ttcattctat
    32521 atgtcattga catgtctcct ttttgtgttg cgcgtgcgca atgaatatta tgattaaata
    32581 atgattcaat ttttcaaaat tcgttaacgt atacaattga ctgtctactg tcaaacaatc
    32641 cacaaagaat gttgatgcat ataaacaatc gatcacccaa attttcagca tcacccaaaa
    32701 attgcaacga aaatattttt taaaaattaa atattatcaa tctttgtgat tatatcattt
    32761 taaatattta agttatacaa tttttaaagt aattaaaaac gatgaaatgt aaaatgttaa
    32821 aaaagagaca aatttaaatt tgagtaataa aataagaagt attcaaacga agatgacaat
    32881 atataaagta acattacttt agacaaccaa acataactga aaggatagtc acaatgaaaa
    32941 ctaaagaagt cgtagcgctc atgaatatat ctcaagacac tttaagatat tatgaaaagg
    33001 ttggtgtgat tccacctgtt aatcgagatg aaaatggata tagaatatac aatgataatg
    33061 atttaaattg gatttattta gtgaaaaatt tgcgaaatgc aggcgtcagt attgaatcgc
    33121 ttatcgaatt ttgtaggtta gcgcagcttc ctaaaaatga aaatattcaa gcacagcaaa
    33181 agcaaatttt aaataagcaa ctcgaagaat taaatgaaaa cttaaagaca attcatgatg
    33241 cgagagattt actacaatat aaaattgata attatgataa tcatattgct aaaatcaatg
    33301 ctagtgataa tcatgatgac aatgttgaac gtctttggga gagaaagtaa acctctggct
    33361 tgctatagag tttgctccat ggtttatagt ggacttacaa gataaaggag gttcatttta
    33421 tgaatcatat tgaaataagt aaagatgtaa agattccagt attaggtttc ggagtatttc
    33481 aaattcctca agaacaaaca gcagaagcgg ttaaagaagc aattaaagcg ggttatagac
    33541 atattgatac agcacaaagt tatttaaatg aaactgaagt aggtcaaggt attgaagctt
    33601 caggtattga tagaagtgaa ctattcatta cgactaaagt atggattgaa aatgttaact
    33661 atgaagatac tatcaaatca atcgaacgat cattacaacg attaaatttg gactatattg
    33721 atctagtgct tattcaccag ccttacaatg atgtatatgg ctcatggcgt gcattagaag
    33781 agttaaaaga aactggtaaa attaaagcaa tcggtgtatc taacttcggc gttgatcgaa
    33841 ttgtagattt aggtattcac aatcaaattc aaccacaagt gaaccaaata gaaattaatc
    33901 cattccatca acaagaagaa caagttgcag cacttcaaca agaaaatgtt gtggtagaag
    33961 cttgggcacc atttgcagaa ggtaaaaatc aattattcca aaatcaattg ttgcaggcca
    34021 ttgcagataa atataacaaa tcaattgcac aagttatttt acggtggtta gtagaacgtg
    34081 acatcgttgt actcgcaaaa tcagtgaatc cagaacgtat ggctcaaaac ttagatatat
    34141 ttgattttga acttacagaa gaagataaac aacaaattgc gacattagaa gaaagtaata
    34201 gccaattttt ctcacatgct gatccagaga tgattaaagc acttacaagc agagaattag
    34261 atgtttaatc ataatataac aaagtaaata atttacatta cgctaacctg aaacttggat
    34321 gttaaagtct atgtttcagg ttaatttatt actcttaaaa agtgaaagga gcaagcaaat
    34381 aagctattat gaatttttat attatgcaat aacttaccta aggttataat tagataatta
    34441 atatgtcttg gaattaatta tctaataagt tgtatagcag gtggcgatat gacatatata
    34501 cccttttcta ttattacatt aattacaggt atcattatgc atatgacaat gtactttgta
    34561 aaatttgaat gtcggaaaat gccattattc atgacgatta tttttacaat ctaaaagcag
    34621 taaagactca taatatagcc aaaaacgtta ataaaagttt gttaaatgat aagggggtga
    34681 gtattggtaa atttattcaa aagcttaaag ggaaaaatcc tacctggaga tatccaaaaa
    34741 ttaaatggac tatttccaaa aataaagggc aatcatatgg tggttcttat tggaagttaa
    34801 taaataacaa aggaaaaaga atagcttctt taactaaaga aggaaaaatc ttaagggaat
    34861 aaggtgttgt aaattatgtg ctttgatata aataattttg atataaaaaa gttgaatttt
    34921 agaaaggtaa aaaacgcaat tcatttaagt ttaagtggcg ataaatacca gtttttggag
    34981 gacggtaaaa ctatcaataa tacttatttt ttagctgtat atgataatgc aataaacatt
    35041 tttgaagatt tgttttcgcc ttctgattct ataaatttgg ttcatgtagt atatgtttat
    35101 aatgaacctt ataagagaac aaatgttata aaaaaattta catcgttaac aatagatgaa
    35161 ttgtattact ttaaagaata taaaaaggag aaggatgata atattttgtg cgaggagttt
    35221 t
//
LOCUS       DATTQF010000016        33446 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.016, whole genome shotgun sequence.
ACCESSION   DATTQF010000016 DATTQF010000000
VERSION     DATTQF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.016"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(29..1156)
                     /locus_tag="VB241_000654"
     CDS             complement(29..1156)
                     /locus_tag="VB241_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP1243977.1"
                     /translation="MKSKYEPLFDKVVLPNGVELRNRFVLAPLTHISSNDDGTISDIE
                     LPYIEKRSQDVGITSNAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPIAVIEEVLKSKEVYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATAREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADDALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1518..1895)
                     /locus_tag="VB241_000655"
     CDS             complement(1518..1895)
                     /locus_tag="VB241_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP1243978.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2310..2903)
                     /locus_tag="VB241_000656"
     CDS             complement(2310..2903)
                     /locus_tag="VB241_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1243979.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2967..3350
                     /locus_tag="VB241_000657"
     CDS             2967..3350
                     /locus_tag="VB241_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HEP1243980.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3481..5886
                     /gene="mnhA1"
                     /locus_tag="VB241_000658"
     CDS             3481..5886
                     /gene="mnhA1"
                     /locus_tag="VB241_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HEP1243981.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFGLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPIQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASKANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTVFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5879..6307
                     /gene="mnhB1"
                     /locus_tag="VB241_000659"
     CDS             5879..6307
                     /gene="mnhB1"
                     /locus_tag="VB241_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HEP1243982.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6307..6648
                     /gene="mnhC1"
                     /locus_tag="VB241_000660"
     CDS             6307..6648
                     /gene="mnhC1"
                     /locus_tag="VB241_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HEP1243983.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6641..8137
                     /gene="mnhD1"
                     /locus_tag="VB241_000661"
     CDS             6641..8137
                     /gene="mnhD1"
                     /locus_tag="VB241_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HEP1243984.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPLRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAITRTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8139..8618
                     /gene="mnhE1"
                     /locus_tag="VB241_000662"
     CDS             8139..8618
                     /gene="mnhE1"
                     /locus_tag="VB241_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HEP1243985.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8618..8911
                     /gene="mnhF1"
                     /locus_tag="VB241_000663"
     CDS             8618..8911
                     /gene="mnhF1"
                     /locus_tag="VB241_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HEP1243986.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8889..9245
                     /gene="mnhG1"
                     /locus_tag="VB241_000664"
     CDS             8889..9245
                     /gene="mnhG1"
                     /locus_tag="VB241_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HEP1243987.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9508..10662
                     /locus_tag="VB241_000665"
     CDS             9508..10662
                     /locus_tag="VB241_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEP1243988.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDDDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLYVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10718..11092)
                     /locus_tag="VB241_000666"
     CDS             complement(10718..11092)
                     /locus_tag="VB241_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP1243989.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVIATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11111..12427)
                     /locus_tag="VB241_000667"
     CDS             complement(11111..12427)
                     /locus_tag="VB241_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEP1243990.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12838..14313)
                     /locus_tag="VB241_000668"
     CDS             complement(12838..14313)
                     /locus_tag="VB241_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HEP1243991.1"
                     /translation="MNFKLNNTISNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVEVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKYDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14444..15652)
                     /locus_tag="VB241_000669"
     CDS             complement(14444..15652)
                     /locus_tag="VB241_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1243992.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16106..16465)
                     /locus_tag="VB241_000670"
     CDS             complement(16106..16465)
                     /locus_tag="VB241_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEP1243993.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16478..16714)
                     /locus_tag="VB241_000671"
     CDS             complement(16478..16714)
                     /locus_tag="VB241_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HEP1243994.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            17031..18095
                     /locus_tag="VB241_000672"
     CDS             17031..18095
                     /locus_tag="VB241_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1243995.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(18155..18478)
                     /locus_tag="VB241_000673"
     CDS             complement(18155..18478)
                     /locus_tag="VB241_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HEP1243996.1"
                     /translation="MEHVSVIVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            18579..18821
                     /locus_tag="VB241_000674"
     CDS             18579..18821
                     /locus_tag="VB241_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEP1243997.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(19083..20258)
                     /gene="dltD"
                     /locus_tag="VB241_000675"
     CDS             complement(19083..20258)
                     /gene="dltD"
                     /locus_tag="VB241_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP1243998.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(20255..20491)
                     /gene="dltC"
                     /locus_tag="VB241_000676"
     CDS             complement(20255..20491)
                     /gene="dltC"
                     /locus_tag="VB241_000676"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HEP1243999.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(20509..21723)
                     /gene="dltB"
                     /locus_tag="VB241_000677"
     CDS             complement(20509..21723)
                     /gene="dltB"
                     /locus_tag="VB241_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HEP1244000.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(21720..23177)
                     /gene="dltA"
                     /locus_tag="VB241_000678"
     CDS             complement(21720..23177)
                     /gene="dltA"
                     /locus_tag="VB241_000678"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP1244001.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(23193..23345)
                     /locus_tag="VB241_000679"
     CDS             complement(23193..23345)
                     /locus_tag="VB241_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP1244002.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(23393..23503)
                     /locus_tag="VB241_000680"
     CDS             complement(23393..23503)
                     /locus_tag="VB241_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244003.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(23756..24715)
                     /locus_tag="VB241_000681"
     CDS             complement(23756..24715)
                     /locus_tag="VB241_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEP1244004.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLATARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTVGIYGMGDIGKA
                     FARRLQGFNTKILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHNPVN"
     gene            complement(24748..25527)
                     /locus_tag="VB241_000682"
     CDS             complement(24748..25527)
                     /locus_tag="VB241_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP1244005.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(25527..25961)
                     /locus_tag="VB241_000683"
     CDS             complement(25527..25961)
                     /locus_tag="VB241_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEP1244006.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            26059..26325
                     /locus_tag="VB241_000684"
     CDS             26059..26325
                     /locus_tag="VB241_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HEP1244007.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(26404..26787)
                     /locus_tag="VB241_000685"
     CDS             complement(26404..26787)
                     /locus_tag="VB241_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEP1244008.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(26916..27833)
                     /gene="lipA"
                     /locus_tag="VB241_000686"
     CDS             complement(26916..27833)
                     /gene="lipA"
                     /locus_tag="VB241_000686"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEP1244009.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(27917..29236)
                     /locus_tag="VB241_000687"
     CDS             complement(27917..29236)
                     /locus_tag="VB241_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP1244010.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLDSIKEEIEH
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVKTSFEEEGKTLMSNPVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEILAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(29263..30090)
                     /locus_tag="VB241_000688"
     CDS             complement(29263..30090)
                     /locus_tag="VB241_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP1244011.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQIAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKTFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(30103..30951)
                     /locus_tag="VB241_000689"
     CDS             complement(30103..30951)
                     /locus_tag="VB241_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEP1244012.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(31251..32318)
                     /locus_tag="VB241_000690"
     CDS             complement(31251..32318)
                     /locus_tag="VB241_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEP1244013.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(32332..33372)
                     /locus_tag="VB241_000691"
     CDS             complement(32332..33372)
                     /locus_tag="VB241_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP1244014.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT        10523 a         5992 c         5011 g        11920 t
ORIGIN      
        1 aatatcatta gtggtaacag tcaaacactt atttatcttt acgtggtaat ggatagaacc
       61 cttcattaaa ttgatgccat aaattaggag ttaagtgatg attatcttct ctctctggat
      121 caaagtaatt aataatttca tcttcccgtc catctttaat tttttcaaca aattgataat
      181 ctagtagtag ctctctacca atggctacta agtcaacacc aacattttca actgcatcta
      241 aagcgtcgtc agctgtgaaa attgaaccaa taccgataag tggcatacga ccatttatcc
      301 atttgtgaat taaaggcagt ctttcttgtc cagcgtattt accttcacgt gccgttgcat
      361 gcgtatccat cattgaaaca tgaatatagt cgattggcat atggctaatt ttattaacga
      421 gttcctctgt aatttccatt gtgattcctg gagactccgc ttcctctgga gataatctgt
      481 atccaactat aaagtcttta ttaccataca cttctttcga tttaagcact tcttcaatca
      541 cagcgatcgg gaatttatat tgatttgccc atacatcatt tcttctatta tagtatggtg
      601 atacaaattg atgaattaag taatgattcg cgccatgaat ttcaacacca tcaaaccctg
      661 cttcaattgc acgtcgcgtt gcttcaccaa aatccttgat tgcttgttca atctcttcat
      721 tcgtcatttc tctagcacta tgttcttgtt tctgaccgaa actttttaaa gaaattggac
      781 ttggtgctac gacgtctcca tcaggtgtta attcaggcaa tgcttgtgca ccgccatgat
      841 gtatttgtac gagtgctttg gcaccgtttt tcttcattgc tgtagctaat cgttttagtc
      901 cttcaatatc actgtcatgc gcgattgacg gctgtcctgg aaatgctttt ccgacatcac
      961 tcacattact cgcagcatta cttgtaatac caacatcttg tgaacgcttt tcaatataag
     1021 gaagttctat atctgaaata gtaccatcat catttgaaga aatatgtgtt aaaggggcta
     1081 acacaaatcg atttctcaac tctactccat ttggtaatac tactttatca aacaatggtt
     1141 cgtatttact tttcattact taattcctcc ttaaaattat tgagttgact ataacatgta
     1201 attttttcat tctccaatta tctgtttata tagttgcaat tcgtttaata ctttaagttg
     1261 tagttctcat ttctaaaata aattaaaaaa acaaatccac atacatcatt gcaaatcaac
     1321 gattctatct aataaagata atggatatgt caccttaaac catttgtacg tgtaaataga
     1381 atcaatcata ttacgttaac gcagacaaaa agagattagt atctaatata atttaataga
     1441 tactaatctc ttttatagtc taagaaactt tcatttcaga ccctttgtat gattttcggt
     1501 acgatttatc tgttccttta gtcgtttcga attgctcgct ttgactgttt tatccagatt
     1561 ggtaagcgtt ctttaagtgt ttgaaaccca tatttttctg tttctctgat ttcatctaat
     1621 actgattgtt tttccttctt acgctcataa tttttgaagt aatcattatc ctttaatgat
     1681 agattgagct ttccttcatc atctatagac aaaattttag ctttaacaat ttggccttct
     1741 gatagaaatt tcttcaaatt atgaacgtag tcatccataa tttctgatat atgaatcagt
     1801 ccttcagtat gattaggggt ctcaacaaac gcaccgtatg gttgaatacc agttacacgc
     1861 accttgatat gttggccaat tttgtagtta ttcaacttaa taacccctta tttcgtttac
     1921 ttaacactat tatcatagca caaatcgaaa tggtggacta attaaaacat caaatcatac
     1981 gaatattatt ttataaatga ataagaatac tacaaacttg gatttacaaa taaaatcagc
     2041 aacaaattcc tatctaaatc aatatgatat ccagctactt tgttttcaat ttattaacta
     2101 tttgaatagc aattctctgt aaaacataaa aagcaacctc aactattatc tctagaatac
     2161 acatctattt aaaaactgtg ctttcttgat aaatatattg agattgctca tcatattagt
     2221 ttatttgttt tcgttggaat atatactaat ccattcataa attagcttta tccgagttta
     2281 aaatgttggc agttaattat gtttagatat tattcttcaa catcaataga ttcaattaca
     2341 acatcatgaa gtggtttgtc ttgtggtccc acttttgtat ttgcaatatc ttctaaagta
     2401 gtttcaccat caatgatttg accgaatact gtatgttttt gatctaacca tggtgtacca
     2461 cccttttcgc catatgcatc aacgattggt tgaggccagc caccatctgc aagttgactt
     2521 aacatatttt gaggtacttc tttcatttga acaatgaaaa attgtgaacc atttgtatta
     2581 ggtcctgagt tagccattga taatgcgcca tataagttaa atgcatttaa cgaaaattca
     2641 tcttcaaaag caccgccata aatactttcg ccacccatac cagtagctgt tggatcgcca
     2701 ccttgaatca tgaagtcatt aatgacacgg tggaatgtta ttccatcata ataaccattt
     2761 tttgcatgtg tcacaaaatt ttcaactgtt tttggtgcaa tatttggaaa taatttgaat
     2821 gtcatgtcac ctttatttgt gtgcataacc actttaattt caccttgttg tacttctttg
     2881 tttaactgtg gatagttagc catttatatt ctccattcat gttatgatac atatatatta
     2941 acatactttt aagggaggaa aatagcatga agctatatcg attttcacac aaaacaggta
     3001 gctatggtgt cacgattgtt gaagaaaatg gagacgatgt ccttgttaaa gttgaacaag
     3061 ttatcaaaca cccgaaacaa ggtgatttac ataaccctac tgaaactgaa ggtgtatttt
     3121 tccatgaacg caaagcatta agtcattttg aaaaacgcta tacgaaacgt tcacaattac
     3181 gcgacttcaa tgttgatgta ttgcgctatg aagattcact tcaacaagca attacagatt
     3241 tagaaactaa acttaaagct cagcaaacaa aacatgctga aatgtcttta gctagtttag
     3301 cacgcttaaa agaagattat tcacttcaat acaaagaaaa ttttaaataa aaaatgacgt
     3361 gtttcttctt atttaaatac ttctgaaaat aaagcacaac aaggagtgtg tacgtttttc
     3421 agaactatat cattcatgta taatgaataa cataaatatt ttttaaagga ggacaacata
     3481 gtgagtttgt tacatattgc ggtgatatta ccgttaatat tcgcactcat tattcctatc
     3541 ctgtatagat tttttaaacg tatacattta ggatggttcg tattacctgt ccctatcgtc
     3601 atatttattt atatgcttac attaattaaa acgacgatgt caggtaatac tgtaatgaaa
     3661 accttaaatt ggatgcctca ttttggtatg aactttgatt tatatcttga tggcttaggt
     3721 ttactctttg gtttgcttat ttcaggaatc ggcagtttag tagtattata ttcaatcggc
     3781 tacttaagta agtcagaaca actagggaat ttttattgtt atttattact atttatgggt
     3841 gcaatgttag gtgtcgtact ttcagataat gttattattc tatatttatt ctgggagcta
     3901 acgtcatttt ctagtttttt acttatttca ttctggcgcg aacgacaagc atctatatat
     3961 ggtgctcaaa aatcgcttat aatcacagta tttggtggtc tatcattatt aggtggtatc
     4021 atcttactcg ctataccaat acaatcattt agcattcagt acatgattca acatgcttct
     4081 gaaattcaaa atagcccatt cttcattttt gcgatgattt taatcatgat tggtgctttt
     4141 acaaaatctg ctcagtttcc attttatatt tggctaccag atgcaatgga agcacctaca
     4201 ccagtcagtg cttatcttca ttcagcaaca atggttaaag cagggttata tttaatcgct
     4261 cgaatgacac caatcttcgc agcatcgcaa ggttggatat ggactgtcac acttgttggt
     4321 ttaattacgt tattctgggc ttcgctaaac gcaacaaaac aacaagactt gaaaggtata
     4381 ctggcatttt caacagtatc ccaattaggt atgattatgg cgatgcttgg aatcggtgcc
     4441 attagttatc actatcaagg cgatgatagc aaaatatatg ctgctgcatt cacggctgca
     4501 atattccatt taatcaacca tgcaacattt aaaggtgccc tatttatgat tactggtgca
     4561 gtggatcatt ctactggtac acgtgatgtt aagaaattag gtggcttact aacaattatg
     4621 cctatttcat ttacaatcac tgttattaca gcattaagta tggctggtgt accaccgttc
     4681 aatggtttct tatcaaaaga atcattttta gaaacaacat ttacagcaag caaagcaaat
     4741 ttattcagtg ttgatacatt aggctaccta ttcccaatca ttggtatagt gggcagtgtc
     4801 ttcacatttg tatattcaat taaatttata atgcatatat ttttcggaca atataaacct
     4861 gaacaattac ctaaaaaagc acacgaagta tcaatattaa tgcttttatc accagctatt
     4921 ttagctacat tagtaattgt atttggttta ttccctggca ttttaactaa ttcgattatt
     4981 gaaccagcta catcttcgat taatcatacg gtaattgatg atgttgaatt ccatatgttc
     5041 catggattaa cgcctgcctt tttatcaaca ttagtgattt atattttagg tatcttacta
     5101 attgtgacat ttagttattg ggtgaaattg ttgcaacgac aacctggtaa actaacattc
     5161 aattattggt acaacagaag tgcaaatgtt attccaaact actctgaaaa gatgacgaat
     5221 agctatgtta ccgattattc acgtaataat ttagttatca tttttggtgc attaatttta
     5281 ttaacatttg tcactgtatt ctcagtacct ttcaacatta attttaaaga cgttagtccg
     5341 attagaattt tcgaagtttg tatcgtaatt ttattattaa gtgctgcctt tttaattctt
     5401 ttcgctaaat caagattatt tagcatcatt atgttaagtg cagttggata tgctgtgtct
     5461 gtattgttta tattctttaa agcacccgac ttagcattga cacagtttgt cgttgaatct
     5521 atttcaacag cattgttctt attatgcttc tatcacttac caaatttaaa tcgttataat
     5581 gaaaagcgct cattccagct tactaatgct ttaattgctg gtggtgttgg tttgtccgtc
     5641 attattatcg gtttaattgc atacggaaat agacattttg aatcgatttc taagttttat
     5701 caagagcacg tttatgattt agctcacggt aaaaacatgg taaacgttat actagttgat
     5761 ttccgtggta tggatacttt attcgagtca tctgtgcttg gtatcgcagg attagctgta
     5821 tatacaatga ttaaattacg taaaaagcgt caaacgcaag gtaacgaggt gaaaaatcat
     5881 gaatagacaa caaaatgatc taattttgca atttgctgca gttattatct ttttcatggt
     5941 aatggtcttt ggtttttcac ttttcttagc aggacattat acacctggtg gcggtttcgt
     6001 cggcggatta ttattcgcaa gttcacttgt tatcattacg attgcatttg atatagaaac
     6061 aatgcgtaaa attttcccat tagattttaa aatcttgata ggtatcggat tagttttttg
     6121 tattgcgacg cctattgcaa gttggttttt aggcaaaaat ttctttacgc atgtcacatt
     6181 tgatataccg ttatttatat tagaacctgt tcatatgaca acagcagtat tctttgattt
     6241 cggtgtttta tgtgcagtag ttggtactgt catgacaatt attatttcga ttggagagaa
     6301 cgaatagtgg aaattattat gatttttgtt agtggtattc tcacagcaat tagtgtctat
     6361 ctcgttttgt ctaaaagtct gatacgaatt gttatgggaa ctacgctatt aacacatgca
     6421 gcaaatttat ttttaataac tatgggcgga cttaaacatg gtactgttcc aatttatgaa
     6481 gcgaacgtaa aaagctatgt tgatcctatc ccgcaagcac ttattttaac agcaatcgtt
     6541 atcgcctttg cgacaacagc ctttttctta gtattagcat ttagaacata taaagaatta
     6601 ggcacagata acgttgagag tatgaaagga gttccagaag atgattgaaa gtaatatgct
     6661 tgtgttaacg ctcgtcattc cagtaattac agccatatta cttgtcttta ttggaaaacg
     6721 tcctattata aagcgttatg tcgcactagg cggtacatta ttaaccttgg tcgcggcaat
     6781 tatcaattta gcaaatgttg taaaacatgg accattacga gttgaactag gttcatggaa
     6841 agcgccttac agtattgtct ttgttttaga catattcagt gccctactaa ttattactag
     6901 tattattatt acagctattg tgattttata ttcataccaa acaattggta ttgaacgtga
     6961 aagatattat tattattttt cagtactatt tatgttaata ggtatcattg gtgcattcac
     7021 aactggtgac attttcaacc tattcgtatt tttcgaagta tttttaatgt catcttattt
     7081 cttattagta attggttcta caaaaattca actacaagaa acaataaaat atgttcttgt
     7141 taacgttgta tcgtcatcct tcttcgtaat gggtgtagcg attttgtatt cagtagtagg
     7201 aacattgaat ctagcagata taagcaataa attagctaac ctttcagcgc atgacagtgg
     7261 cttagtcaat atcgttttca tactatttat ctttgttttt gctacaaaag ctggcgtatt
     7321 cccaatgttt gtttggttac ctagtgcata ctatgcacca ccaattccaa tcattgcatt
     7381 ctttggtgct ttgttaacaa aagttggtgt ttatgcaatc actagaacat tgagcctatt
     7441 ctttagtgat aatgtaagct tttcacatta tgtcatttta ttcttagcac tattaacgat
     7501 catctttggt tgtgtcggtg ctgttgcata tgccaacatt aaaaaaatca tcctatacaa
     7561 tgtcatgatt gctgtaggtg tgatcttagt cggcgttgca atgatgacag agtcaggtat
     7621 gattggtgct atttactata cattgcacga catgttagta aaacttgcat tattcttgct
     7681 tatcggaatt atgattaaaa ttacaggcac tgctgattta agacaatttg gtggcttaat
     7741 taaacgatat cctgtacttg gttggtcatt ctttatcgca gcattaagtt tagctggcat
     7801 tcccccactc agtggctttt atggaaaatt tttcattgtc caatcaacat ttgaaagagg
     7861 attttattta agtggtgtca tcgtcttatt atcaagttta gtcgtattat attctgtcat
     7921 tcgcatattc ctacaaggct ttttcggtca accaaaagga tatgatctta ataacaaagt
     7981 cgacgtcaaa tatttaacta cgattgcaat cgttgcagtt gtgataactg ttttatatgg
     8041 attgtcagca gattacttat atccaatggt aaaagctggt gcagaaacat tctacaatcc
     8101 atcaacctac gttaaagctg tattaggagg taaataacat ggctgtacaa ttagttttaa
     8161 actttattat tgcagtgttt tggttgtttg tgacaaatag ttacacaaca aataactttg
     8221 tgttaggttt catctttggc ctagttcttg tttacctctt acacagagtg ttaccaggta
     8281 gattttatgt catcacgcta tatcgtatta ttaaattagt tattattttc ttaatcgaac
     8341 tgattaaagc taattttgat gttttgaaaa ttattattaa accttcgatt aaaaatgaac
     8401 caggattctt tgtttatcac acagatttaa aaaaagattg gcaaattgtc ttgttgtcta
     8461 atttaattac attaacacct ggtactgtag tgcttggtgt aagtgatgat cgtacaaaaa
     8521 tttatattca tgccatagat ttttcaacta aagagcaaga agtagaaagt attaaaacat
     8581 cattagagaa gattgtaaga gaggtgggcg aaatataatg aatcataatg ttattatcgt
     8641 tattgcatta atcatagttg tcatttctat gttagctatg ctcattcgcg ttgtgctagg
     8701 cccatcactt gccgatcgtg ttgtcgcatt agatgcgatt ggtcttcaat taatggcagt
     8761 tatagcatta ttcagtattt tattaaatat taaatacatg attgtcgtta ttatgatgat
     8821 tggtatatta gcctttttag gtactgcagt attctctaaa tttatggaca aaggtaaggt
     8881 gattgaacat gatcaaaatc atactgatta gtcttgcact tatctttgtt atcatcggtg
     8941 ctttaattag cgccctagca gccataggat tattgagact tgaagatgta tattcacgtg
     9001 cacatgctgc cggaaaagca tcaacattag gtgcaatgtc attactattt ggtacgtttt
     9061 tatattttat tgctactcaa ggttttgtaa atatgcaatt aatcgttgcg attatctttg
     9121 tattaattac aggtcctctt tcaagtcata tgattatgaa agcagcatat aatattaaaa
     9181 cgccttatac taaaaagact aaagtcgatg aaatttctga agacttaaaa gacacaaaat
     9241 tatagattat ataacaaagt catgaatata aatcaacaac aaacagcagt aagatgattc
     9301 caaattagga atgattttac tgctgttttc ttttgacatt gttacctctt tttcaatgat
     9361 tttagtttag tacatacttc tttgactaca ccttcgccct atctaggtat atctctttaa
     9421 tttaattgcc tttcatgtcg ttatgtatta tgataataat gattataaat cgtaacgatt
     9481 acgctttaaa aagagagagg ttttattatg cattggacaa ttatcggcgg tggcatacag
     9541 ggaactgcaa tcgcacaaaa actattatca agcggattaa caacagaccg attaacaatc
     9601 attgacccac atgaaacttt ttgccaaagg tttaactcat atacaaatcg aatagaaatg
     9661 ccttatttaa gatcaccgat tgtacatcac gtacatccac aaccgttcca tctaaaacaa
     9721 ttcgctaaac agcaccaata tacaaatgct ttttatggtc cttatcagcg acctgaattg
     9781 acaatgttta tggatcatat tgcacatgct tctaaacaat atcaattaga ggattgcttg
     9841 gttcaaggtt tagttcaaac tttagataaa caagaagaca aatggcatat caagttagaa
     9901 gatggacaaa ttatcactac agattgcgtc gttattgcaa taggcagtac aaatattccg
     9961 tttatgcctg acattttaaa agacaaacag aatgtaaatc atatcttcga gaaagaacat
    10021 gatcaagtag tatatgataa gaccgatcat atcgttggta gtggtattac tgctgcacat
    10081 cttgcactta aattgttaaa tcatgatgac gataaaaaga ttcatttatg gctaaataaa
    10141 gatattgaaa tacatgactt tgatgctgat cctggttggt taggtccgaa aaatatgtct
    10201 tcatttttaa gtactaaaag tatgcctgaa agaaatgcca ttgtacaacg tgaacgtcat
    10261 aaaggatcaa tgcctcacga actgtactta cgccttaaaa aacatattaa aaatggtcgt
    10321 ataaatgtgc ataaaacacc tatcactcaa attagtgatg gtttaattaa cactgaaaat
    10381 gattctgttc catatcaaca gattatggtt gcaactggtt ttgaacaaga ttttatgtca
    10441 caaccactta ttaagcaatt aatacaaaat tatgatgcac ctatcaacga atgtaattac
    10501 cctgttattt ccgaaaaatt agaatggata ccaaatctat atgtcgcagg atgctttgca
    10561 gacttagaat taggaccatt tggtagaaat gttatgggtg gccgtaaagc tgccgaacgc
    10621 attgaacaag catttctaaa attacaacaa tatagcgcat aaaaaaactg ctagccgtag
    10681 ttatgaatat ctcataactt ttcagctagc agttctttta ttttaaaggt ttaatagcaa
    10741 ctgtaccacg cataactgta attaattgtt ctttgtcatt cttaattttt atatcccata
    10801 catgtgttga cttaccttga tgaataattt cagctgtcgc aataacacga ccatctttag
    10861 cagaatgaat atgattggca ttcatctcta aacctaatgg aataaatttg gttgtatcaa
    10921 ttagattagc agatcctaat gaacatgctg tttcacctaa agcaatcgaa gcaccgccat
    10981 gtaaatatcc aaatggttgt tttactttat cagtaacagg catagaaata acaactaaac
    11041 catcttcctg gtgatctatt gacatctcaa atgtctctaa taaatgagtc atattttcac
    11101 ctctttatgc ttatagtacc atagcagcaa tagtaccgaa aatcattaaa ggaatattat
    11161 aaaacaagaa gtttggtaca catgtatcgc gtatatgatc atgttggcca tcaacattta
    11221 atcccgcagt tggtcctaat gttgaatcac ttgcaggtga acctgagtca cctaatgcac
    11281 tcgctgttcc aatcaatgcg attaatgcca ttgtatctag tccaattgac gctccaaaag
    11341 gaatgaataa tgatgcgata ataggaattg ttgcaaatga tgatccaata cctaaagtga
    11401 caattaaacc tatcacatac atcatgataa tgctaaataa tttattatca ccagtaatac
    11461 ttgttaaagt tttaactaat tcatctatat caccagtagc attcattaca ccagcaaatc
    11521 catttgctgt taaaataact acaccaatat aagccataat tttaatacct tcaacaaact
    11581 tagcatctaa ttcataccaa ttatatgcac gtgaaataaa gaatacgagt accccggcca
    11641 gtgcaccaaa aatcattgaa tctgtaaacg tttgtactaa aaatgtagct agtattgcca
    11701 caattgttac tattaagata tatggtttta actctgtaac attgtcacta tctgaaattt
    11761 tacgtgtttc atattcacgt ggtttacgat atacatataa accgataagt aagccaacaa
    11821 tataccccat tgaaggaata agcattgctt tccaaatcat attaaactca attgggtgat
    11881 tcgcctttgc aaagccactt tgaataattt gctggaaaat ttgaccgaat ccatatggta
    11941 ataacacata cgggaaacat aaaccaaaac cgataatcaa accgattaaa cgtctatcta
    12001 tttttaagtc attaaacaga cttaataatg gtgggatgac aattggaatg aatgcaatat
    12061 gtacaggaat taagttttga ctcatgatac tcatagctaa taatgcaatg attattgtca
    12121 ctttcacttt aactcttgac catcgactat tttcagcgtg aattgcattt attatttttc
    12181 ctactaaata gtctgtgata ccactgtatg aaattaatgc tgcaaatcca cctaataagg
    12241 catagcttaa tgctacctca gcaccatcga ctatattttt cccaaataca tttataactt
    12301 tttcaatgct catgcctgaa attaagccac ctactagcgc actgataaat aagcttataa
    12361 ctacgtttaa tcgacataaa catagcacaa tcattaatat tactgctatt actactgcat
    12421 ttatcatatg acaaccaccc tttatcactc tatcgaacta aagcgtttat agactacata
    12481 gtatcagtag ctttaacacc tgtcaatagc aaactatacc aataatcttt attttttagt
    12541 taattgtctt tcttaaaata attttcgctt tcattaaatt aaacaatttt acaagtctgg
    12601 aacaccaatc aaaatcctaa gttctataat gttatattag tagtcgttga ctgaatgaac
    12661 atatgcttat aataagtttt tgcaatgcta atcaagttga tttatgctca caaggatatg
    12721 cgatttatat tttcttacaa caatgaaaat gcctgataca atgcgatcct ttatactttc
    12781 aagagttaaa ttagaaatgt ataacaagac acaagttgta tcaggcaaat aattaaatta
    12841 ttgttgtttt aaccattgta ctatcgtcgg aatcataaat cctgttggcc cttttggacc
    12901 attatatgaa gctttattag tcgttgctgg acctgcaata tcgaaatgaa tgtgaggtgt
    12961 ttgaccacta aaatgtgtta caaaacttgc cgcaaatagc gctttacctt gtccattcgt
    13021 atggttaact aaatcagcga tatcactgtg tttaatactt gcacgttcgg ttgcagtaat
    13081 cggtaattca aataccattt catcgacttt agaacttatt tgtaatatgt cgtttaatat
    13141 cactttacta ttcgattcaa atgcagcagc tttatcatcg ccaagtgcaa cgatggctgc
    13201 acccgttaat gtagcaaagt ccataatcac actaggctga tattgatttg cataatacac
    13261 agcatctgca aggactaatc taccttcagc gtctgtattc attacttcta cagtttcacc
    13321 acttaacgct gtaaatacat catctggctt cattgatgct tcatttatca tattttcagc
    13381 acaagcaagc actccgacaa tatttacagg cagttgtaaa cgactagccg cttcaatgat
    13441 accaacgaca ttcgcagcgc cacacatgtc atatttcatt gtagccatgc cattcttcgt
    13501 tttaatacta taaccaccag aatcatacgt tataccttta ccaactaagg caattggtgc
    13561 ttcatctttg tctttgccat tatatgtgat ggttactaat ctcggtttat gcttactacc
    13621 tttacctacc gcttgtaaaa gtccaaatcc ttcagaaact aaagtgtcat aatctttaac
    13681 ctctactttg acctttgtat ttttaaaatg attaacaata tcttctgcaa atgtttgtgg
    13741 tgttaataca ttcggtggca tattactaaa gtctcttgcc aaattaatgg actggccaat
    13801 actgatacct tcatgaataa aatctaattc aattaatgat tcactaataa ggtttaaatt
    13861 tgtcttaaac ggtgccttct tacttgattt ataatgatcg aattcatatg ttgcacgctc
    13921 actttgaata ccgcatgcca ttaatacatc tgataactga tcatattttg aaataaatga
    13981 atccataagt aaatacgtat cttcaatgtg ttctgacttt atgtattgga aaagatgtcc
    14041 ccatattttc aacatatctt gataactacg tgtttttaag tttcctaaac caactgtaat
    14101 taaacgatat gtttgatctt gtacatcaaa tgcagttgta taaatcttcc caactttact
    14161 accaataata tgttgatgtt ttagtctttc aagtgattct gtaatatcga tatgattaaa
    14221 actaatgcgc tctaactgat ttaaatgttc tggtatgcca ataatcaatg tatttatttc
    14281 gttgcttatt gtgttattta atttaaaatt cataatgtac ctccttagat ttgatatgtt
    14341 actcacttca aattgtacaa caataaagcc cttcagtgac actgaaggac tttataaatg
    14401 aataatttaa ttttatgtgt ttagctgtaa cttaatttaa agcctagaat ttaccttttt
    14461 tgaaagctaa accgatacca ccgattttga ataccgcacg tgtatcaatc acttttttca
    14521 tgaatgctgc ttttttacca gcgataggtt taccaaatac catacctaca ccgtcatgtg
    14581 aacctaaaga acaaacagtt ccacgatcaa cgtattcgaa ttcttcagtt gattcaccgt
    14641 ttaagatgcg tttaatgttt ttagcaacac tttcaccttg ttgcattgca atttgtgctg
    14701 tagttggtaa tggacgctct tctccagctg ggataaacgc tgaacagtca ccaataacaa
    14761 aaatgttgtc gtaaccattg attgttaaat cttgctttgt aacgatacgt ccacgtttaa
    14821 cgccttcaaa tgattcttcc attaatttac taccacgtac accagcagcc catactgaag
    14881 tacctgcatt taattgttgt ttttcaccat ctacttcaac tacgaaacct ttttcgttac
    14941 aagcaacgat tggtgtagca attttaaatt caacaccgcg gtcttctaag tagctaactg
    15001 cgtggttaac taattcttct gagaacattg gtaacatttt aggtgctgct tcaacacaag
    15061 tgattttaac tttattttga tccacaccat atttgctaca taattcagga attctgtctg
    15121 ttaattcacc taagaattca acaccagtga atccagcacc accaactaag atagataaat
    15181 cgttatcatc tttttctttt gatgctgcat agttagcaaa tttgtcttcg atatgacgtg
    15241 ataattcacg tgctgtgata acattttcaa tttggaaagc atgatctttc ataccttcga
    15301 tgccgaatgt ttcactaacg aaacctaatg ctactactaa aatatcaaag tcataaatac
    15361 cttgatttgt ttctaccttt ttagcatcac ggtcaatttt tgttacttct gcttgaacaa
    15421 agttcacttt gtctttcttc aagacacttt ccacaggata taatacatct tcatagttta
    15481 gtgtacctgc tgatgcttca tgtaaccatg ttgcttcata gtgatattca tttttattaa
    15541 taagcgtaat ttctgcttct tctgttgata tcgctttttg caatttagtt acagtttgta
    15601 aacctgcata accagcacca agtacaagta cttttttacg atcttgagcc atttaatttc
    15661 acctaagctt tcatattttt ttaaccaaat gctgataatt actagtaaca actggtcatt
    15721 actactttta ccacaatgta tactagttta tcatcatttt gttatttaag tcttttaaat
    15781 tatgtaaaaa cctgtgatgt tcacaaaaaa gacattttta atccaatact aattctatag
    15841 cttttatgtt atattttcaa gcaagattca gtcagattgt gaaaaaatta attaattttt
    15901 tcgtaacctg atttcgatta aaaatgtatc cgttatcacg atcatttaaa atactattaa
    15961 tagtcagatt cttaattttt aatgagattt ttaataaaac aaaatatctt tacgcaaaaa
    16021 taggacataa tcaaatatgt ccctaatcgt aattgatatc aatttgtatc cccaaaattg
    16081 ggttaatcat taaaaagtta ttttattagc agttttcagg attacctgca acttttgcag
    16141 ttctaaatga actaccacag ccacatgatg caattgcatt aggattgtcg atttggaaac
    16201 cgccacccat taatgattgc ttaaaatcaa tagtcgtacc atttaatacg ggtgcatctt
    16261 ttttgtctac taaaactttt aatccaaagt attctaagac ttcatcattt tcaccaggcg
    16321 cttcttctgc acccattccg tatgttaaac cagtgcaccc gccaccattc actttaattt
    16381 ttaaatagcc atctggcatt tcatttgctt taagcatatc ttttacttcg taggcagctg
    16441 cttctgttaa tataactgtt ggcatgataa ctcctcctta aaaaatccaa gtttctttta
    16501 tatgtgcata tatattttgt aataattctt ccggcgaatc accttcaaca atatcaccat
    16561 ttactaaagc atacaacccg gctgaacata taccacaatg tgtcaggcaa ccatactcta
    16621 acacatcgac atctgggtca ttttccagtt gattaaaaac ataatctcca ccttttgcca
    16681 tgttagagag acaaaattct acgatcggat tcatacttca ccttcttatt tcatttgtta
    16741 caatattata gcattttaaa actggtattt taacatgatg tgctcaatta gcaacaactg
    16801 atgtttctta tcccagttat gtaatagtgc cttagttagg acaaatattt atttgtagtc
    16861 attttcaaaa aaatatataa taagcatata aatacatatt tactttttta ggggttattg
    16921 atgaatcaaa gacacttgca ttttataact gttcattaac aaatatatct atgcactttg
    16981 atcttttcaa aattaaagta aatattaaca tataaaaggg gtacttcatt atgaaaaact
    17041 tagttttgtt aggcggcgga tatggtaata tgcgtatcat gtcacgcatt ttaactacat
    17101 ctttaccaca agattataca gtcacattag ttgatcgtat gccatttcat ggattgaaac
    17161 cagaatttta tgctttagct gcgggcacga aatcagataa agatgttcgc atgaaattcc
    17221 ctaatcatcc acaagtgaat acggtttatg gtgaaattaa cgacatagat ttagatgctc
    17281 aaattgtctc agtcggtaat tctaaaattg attatgatga gctaattatt ggtttaggat
    17341 gtgaagataa atatcataac gttccaggag ccgaagaata tacacatagt attcaaacac
    17401 tctcaaaggc tcgggatact ttccatagta ttagtgaact accagaaggt gctaaagttg
    17461 gtatcgttgg tgctggatta agcggcatag aacttgccag cgaattaaga gaaagtagat
    17521 cagacttgga aatatatctt tatgaccgtg ggccacgaat tttaagaaat tttccagaaa
    17581 aattaagtaa gtatgttgcg aaatggttcg ccaaaaataa tgttaccgtt gttccaaatt
    17641 caaatattaa taaagttgaa cctggtaaaa tatataactg tgatgaacct aaagatattg
    17701 atttagttgt atggacagca ggaattcaac ctgttgaagt tgttcgtaac ttgccgattg
    17761 atataaatag taatggacgt gtgatagtta accagtatca tcaagtacca acatatcgta
    17821 acgtctatgt agttggtgat tgtgctgatt taccacatgc gccaagtgct cagttagcag
    17881 aagttcaagg tgatcaaatt gccgatgtgc ttaaaaagca atggctaaat gaaccattac
    17941 ctgacaaaat gccggaacta aaggtacaag gtatcgttgg gtcattagga gataaacaag
    18001 gttttgcata tatcatggat cgcactgtaa caggacgact cgcttctatt ttgaaatccg
    18061 gcgtactttg gttatataaa tatcataatg gttaaaaatt aaaaagcaat gtcagttcac
    18121 actattactg acattgcttt ttattatgtg tatattattc attcacaagc ttttggtcta
    18181 taaatcgagt gatttgcttt gtttgtatat aaccatctgc tacatattca tcgttcattg
    18241 taattaatgg ataaaatagt tcatcttgtt ctattctttc aataaattgt aaatcatgat
    18301 ctgttaagtt gtcattatct tttgtaatat ctatatacgt atatttaaat gatatatttg
    18361 ggtattttct ttttaatagc ggctgtagcc agtcataaat atctttcgat gttggcgcat
    18421 taacgcaact tgcacatata acatctgccc catatactat cacactcacg tgctccatat
    18481 atttatcccc cattggtttg atagattttt attacactat ctattataat atactaataa
    18541 agattatcaa attcatctct cgaaaggaga cttgcctgat gcctactgaa gatacaacga
    18601 tgtttgatca agtagcagaa gttattgaac gtcttcgccc atttttatta cgtgatggtg
    18661 gcgactgctc attgattgac gtggaagacg gtattgttaa attacaatta catggtgcat
    18721 gtggtacatg cccaagttct acaatcactc ttaaagctgg tattgagcgt gcattacacg
    18781 aagaagtgcc tggtgtaata gaagtagaac aagtattcta atataaagat attaagttaa
    18841 cagaacatat tatgaataac aacaaagaca ggaatactga attatttata atcagttatt
    18901 acctgtcttt ttcgctgtca aattggactg atgggaaagt tattttatac tatttatctg
    18961 attaattatt ttataatagc cacatatcca agcgcatatt actattctat tctatgaaat
    19021 ttaaaatata aaaatagcac ataccctcaa tcaaaatcgt tgagttatgt gctatttgta
    19081 ttttaatttt taggtttatc tacttcaggt tgtggttcac ctttcatatg tttcgcaatt
    19141 tgctcatcca tataaaccca acctttccaa ccgatgtgta cggcatcact gattacatat
    19201 ttttcataat ctttatcagt catatcgtaa attttaccac cattatctac aacagtagaa
    19261 tggatttttt tataaactgc ttgacgacgt tctttatcga taccaatgtg gtcataccat
    19321 acaccgtttg atggaatact tacatattga acatctgcac cagcagcacg cattgttttt
    19381 acaagtaatt ctaaatcttg gaattctgga gaattaacat tgaattcata gtcacgtcta
    19441 actttacgct tactttcttg aattaatttc cagtattgat ctctaatacc aaatttattc
    19501 gatgtagtat cagctttacc aatttcaact gctttttgtt tcatctcatc ccaagaagcg
    19561 tctggttttg tagcaggttt aacatgttct aatggagatt tatccattgc aaacaatgat
    19621 ttaatcgctt ctattttaat caattgattc tctttaaaac cagaaatata actatcttta
    19681 gtttctttag ggtttttagc ataagatttc aagtattctt tattgtgtac atgtggaaac
    19741 tgtaataaac gttgtgcata acgacgtttt aattcagtag acatgttttt ctgctggaac
    19801 atttgattaa tttgagtttg agacatacga gcatcaaagt tttgattcgt taaaccatga
    19861 tttgtaaacc attgtggtga aataataaat gttaatttct taccttttaa tttatcatac
    19921 tgtgatgcaa gttcaactgc gttaattaag tctgtagaac caccagcacc taataagaat
    19981 gcttttttgt tggcgttatg cttatttaat gcaattgcag gattaaatgg gtcatcttta
    20041 cctaattcac tagagccgta tataggataa tacttgtttg attcgtataa cttatcttga
    20101 atgagtgtgc cttttagtac ttgatctgtc aatgaagttc tattatcttc tacagtcttt
    20161 tcatttacta atcctgtaaa ccaactagca ggtaataata gaaagacaat gaataccgct
    20221 ccactaatta aaatgggtaa aaaaggtttt aatttcatcg taactcttct aatgcttcaa
    20281 cgattttatt tggtgttgcc cactcatctc tatcaaagtc cataatagat acttcgatat
    20341 caagtttatt ttgaatctct aataataatc caactgtttg gaaagaatca ataatacctt
    20401 cttcaaaaat ttctacgtct ggattttctt ttacaatatc attttctgct acttctgcta
    20461 ataaatttaa tacttgttct ctaaattcca taattaaatt ctcctttatt atataagttt
    20521 acctgagaag attaaaaagc caaatgttac aaagtggaat gtaatcacaa tgctaagtgc
    20581 tgttgtgaaa ccattttgcc aacgtggcgg atgtttcttg cgccaacgtt cataatagcc
    20641 ataacctata aacaatgctg catggtataa accataaaca atgtaataca cttcgatacc
    20701 atgccaaatt cccattatga agaagttgat taagaatgcc acgttagaca ttgcaaattg
    20761 actcttcaat aattttttac gagacatgta gaataaagat ctcatgtaaa tacaatctct
    20821 gaaccagaat gataatgtca tatgccatct attccagaaa tctttaatat ttttcgcttt
    20881 gaaaggttta tcgaagtttg gtggtgtttt aataccgaat aaataactaa atgctatcgc
    20941 aaataaacta taacctgcaa agtcaaagaa taaatatagg ctgtatgcgt acatatataa
    21001 ccacaaattg acaaagccat gtaagtctaa ttgtaacggc atgattgcat atgtgttaat
    21061 aaagtaagca acaatatatt tatacaagaa accaagcata atcatgtgaa ttgcttttaa
    21121 tactaattca cgatattcat tgcctgttgg tactttttta tcgtctttaa cgaaacgttt
    21181 gtaacgatcg attggtccag atgaaatcgt tgggaagaat gaaataaatt gaattaattt
    21241 ccatacttta atttctttga tagaaccatc acgaatttcc ataattaact gcacactttt
    21301 gaatgtaaca taagaaattc ctaagaaacc aacaaattca attaatttac tttcatggaa
    21361 gtgaatctga tgtccaccta accatgtact ttgtaacact ttcacaagtg ctaatggcaa
    21421 tattgataaa accattacag ttacaaattt tgaaaatgaa ttattttttg gttttgaatg
    21481 ataataaaac attatcaata aaacttgcca tacgacgtaa ataataaaac taattaattg
    21541 aacacttaaa tacttttggt caaacagatt atgtttatca gaagaaaaga ttaacacaat
    21601 cataaatgct gtaactacgc cattataaat gtaacttcgc ttacctaaaa atccaagtat
    21661 aatgactggt aataatgcaa ttaaagcaat taagaagaat gtaaaatcac catatggaat
    21721 catccgttaa ttacctctgc aattttcttt ctgtcaattt taccatttga agtcaatggc
    21781 aattgttcca tccattcaaa ctttctagga atcatatact ctggtaagcg tgatttcaag
    21841 tcatttttaa tatttttagt catttctgca ttgtccgtaa cttcagtcgt tggcacaatt
    21901 gcaccaatta aatgaataac tttatcattt ttatatacag gtacaacaat cgcttctttt
    21961 acgaactcag actggcgtaa ttgtgtttca atttcttcta attccattct gtagccattc
    22021 aatttgattt ggaaatcaat acgaccttga atgaaccatt gaccattttc aaacttcgct
    22081 ttatcaccag tgtgataagt acgaataccg tcatcgaaat taaatacttc agcagttttt
    22141 tggtcatttt ttaagtatcc taaacttaca ctttgacctt cgataacaag ttcaccttca
    22201 tctgtcgtag ataatcttgc gcctggtctt tcaacgccaa caggtaatgt cggatattga
    22261 tctaagattt cttgtgtaat ttgaatactt gtaactgcta ccgtagcttc agttggacca
    22321 tatgtgttgt aaatcgtcgc acttgggaaa cggcttacta acgcttttgc tgctctgtga
    22381 ggtagaattt caccacagaa gaagaattcg ttaagactac catactgttc ttcattaagc
    22441 gttggtaata ataaacacat ttccataaat gatggtgttg atacccaaat gttaatcggt
    22501 gttgctgtta gcatttcatt taatagttta ggtttattaa tcatgttttt atctacaaga
    22561 tttaatgtac cgcctgacgc taaacatgga taaatagcca ttacagataa atcaaatgaa
    22621 aatggcgctt ggttaagcca ttgttgttca tttcctgatt tattaagttc taacatccac
    22681 tcagtaaatt gaactaaact tgcatattca atttgaacac ctttaggttc cccagtagaa
    22741 ccagatgtaa agattgtgta tactgtgtcg ttatctttaa tctgactatc aaaaattact
    22801 gggtcttgag atgttttaat atcttctatt gtaaatactt cacctgctaa actttcaaac
    22861 gattcatcag tcgtattaaa tacaaactct ggttgaacct tgttaataat cattttaata
    22921 cggtcttcag gaattgaagt gtctacaggt acatatccac atcctgcttt aatggcacca
    22981 atcatcccaa caatcatata tggtgacatg tgaccgaata aaatcatcgg tttcttacta
    23041 ccttgtaatc gatgtgctaa tttactagac tcatccatta actgttgata agttaattca
    23101 tcagttgtgt gtctaacagc aatgctttgt ggatttgcat ccgcaaacgc ttgcagcttg
    23161 ttaataatat ctgtcatatt aagtctccct cattagaact cattataaat gaagttattg
    23221 tgtgtgtcgc cactgccata aattaaatat aaagtaataa atattgccaa atacaatagt
    23281 gttaataaat atggtttgaa tgcttcaaca tatttattag gtggctgttt acttttagat
    23341 ttcatattgc acctcttaaa gttcttagta aaaacgcctt tataaagacc gttcaatata
    23401 aaatacgttt taaaatttgt tttttacaat tcattatatc gatattcata atgaaattca
    23461 attttaattt tatagattca acatagtaat tggttgtcat cactcaatta ttgtaaatga
    23521 tacactatgt tgagaacatc attcatttta aaggttattt aaacaataaa caattacagt
    23581 ctatataaca attttgttat atacgtcaaa atcaaataaa ctcatcacat taatatgacg
    23641 agtttataac gttattgaat tatcatcagc gcaaatatat acattcgcat gtcaagcata
    23701 acatatttaa caattgcttt gcttgtttac caatgattaa aaaccatact tattttcaat
    23761 ttactggatt atgtggtacc tgatttgtca taaccgcttc tatattatta atacataatt
    23821 gaatcatatt gtcccgtgtt gttactgatg cgctaccaat gtgcggtgta atcagaacat
    23881 tatcacgtcc cattaatgga tgtgtatgat caattggttc atttgctaat acatctaaac
    23941 cacatgctaa tatttcttta ttgtctagtg catcgattaa tgctgtttca tctacaattt
    24001 gccctctacc gatattaata aaaattgcat catttttcat ttgttcaaat gcttcagcat
    24061 taaatttatg atgtgtttct tttgtaagtg gcgctgtaca gatgataaaa tcactttctg
    24121 ctaacaacgt ttcaaaagaa acatatgttg cattaaagtc cgcctctgca tctttatgtc
    24181 ttgatcgatt atgataaaga attttagtat taaacccttg caaccttctt gcaaaagctt
    24241 taccaatatc tcccatacca tatattccaa cagttgaatt gaagacatct ttacctgaca
    24301 ataaataagg accccagctt tgccatgcat ctgcttctac atatttttca gcttctacaa
    24361 tacgacgtgc tgtagcaagc attaatgtaa atcctaattc tgcagttgtt tcagtaagaa
    24421 catttggtgt attcgtgaca accacgttgt tcgctgttgc actttcaaca tcgatgttgt
    24481 catatcctac agccatattc gcaattactt ttaaatttgg tgattgtgct aaaatttctg
    24541 catcgatctg ttcacttaat gtgataaaac aagcatctgc gtcacgaagt gctgtcacaa
    24601 attgatcttt aggcataggc actaatgatt tttgccacat aacaacgtca ccaagtttac
    24661 tcaattgttg ataaaattta tctggaattt tcctcgaaac aactattttt accaaatcaa
    24721 ttcacctact ttataaaatg actatatcta tttttctaat tcagctattg cttcatttaa
    24781 atctttaaaa gaatacgttg gtggcacatt tttattttgc acatcttcta acgtagatac
    24841 acctgtttgt acatgaatcg tatccatacc tacgttaata ccagacataa tatcggtatc
    24901 gtacaaatcg cctaccatag caacttcgga tttatctagt cctaaaattt ctaatgcttt
    24961 aaccataata atcggttctg gtttaccaat aaattgtggc gatacacctg tcgatacact
    25021 tacaacactt gtaatagcac cattccctgg taaaaatcct cgctcttttg gaattgacac
    25081 atctggattt gtagaaataa atgttgcccc atttcttaca cctaacgttg caatcgcaag
    25141 cttttcatat gtaacttgtt cgtcaagtcc aataactaca taatcaacat gctcgtcatt
    25201 tttaatgaca agtcccgctt cggttaacgc agtatttaaa ccactaccac ctaacatata
    25261 tactgatgct cctggtgatt gttctgaaat ataatctgca gtggctaacg ctgacgttac
    25321 aacctcttct ggtttagcat caatgtgcat ttcacgtaat ttttcagtta cttgctcagg
    25381 tgttttcgtt gaattattag ttacgtataa atgaggaata ccttttacat ttaaataatc
    25441 gatgaattgt tttgctccat caatctcatc tgttcccata tacattgtgc catctaaatc
    25501 gattaaatac gctttatact gtttcattat tgattttctc ctttaccaaa agctgttact
    25561 ggtacatttt cttgatgtaa aaatgtcgtt acttctgtaa taaattgttt gtaataaggt
    25621 aatgcgtcat caaacattgg catgataacc ttaatatcta aggctgtgta atcatatgta
    25681 aattgtttac gaatatcgac agttttatta atcgcctgct gtgtttcttt agtaataaca
    25741 ttttctagtt ctaatatatc aatcacatct ttataattac caggatccct taaaataaat
    25801 gcatcgataa tcatattccc tatatctacc gatgattcta tcaacatttg agcaatgcgt
    25861 tcaaatgcat aatgattgtg cttactctca tgataatcat cagttaatgc ttgtaaatag
    25921 gctaatttct gagttagttt atctttgtct acaaaataca ttccttgtca cctctcgctt
    25981 atatattatc ataattttgc agtatacttc atttgtcgtt atactaactt tactactttt
    26041 aaaaggagtc gctacaaaat gattgatatg tatttatatg atgacaacga agagagtcaa
    26101 gttcagtttg ttggttttgt tggcgagcat agtcgatatg atttaatgct agtccataca
    26161 aatagacatt atggtaagac actcgtactt aatatgcaaa caaataaatt cggtattatc
    26221 ggtactgatg atttaaaaga agaaggctat attgctcata ttttaggtgt aaatgctgaa
    26281 gaaggcgatg aaattacaga gtatttaaat gaagtcattc attaaagata aaaaccccta
    26341 actagattac aggtccatgc ttgaattgca tgattcccat tttcaatcta gctaagggtt
    26401 ttattaatgt tcagtatctt gaatggttgg cttaatcttc acatcttgaa gtttattgtc
    26461 gtcatctatc acatcagacg gatgaacttc ttcgattaac tttttaacct cttgtttaga
    26521 caaatgtctt aatacaaagt aaggacaacc aaagttacaa tattcaaata tgtaatcttg
    26581 aatatttgaa aaacgtttac tcatctctgc ttttttatta gaatctttgt aaaaaccttt
    26641 taatcgtaat tgatcgtaac catagtcacc aacaatgtaa tcatatttat ctaaaatatc
    26701 tgaataccta gcaataaatt gttcttcatt aaaacattcg cgataatttt ctattaattc
    26761 aaagtaatgt tgatctactt ttatcaaatt cttcacctat ctatgacaat gaaaggctca
    26821 gtttatactt tagtgtttca taaaaataat gattacttct atgacacgtt cgttttgtac
    26881 aaactaagcc tttatgcctt attaatggtt aaatattaac tatttaactg tgcctcacct
    26941 tggcgttgct tttctttagc agcttcattt acttgctcat ccgcatgata tgaactacgt
    27001 actaaaggtc cagcttgaca atgtttaaac cctttatcca ttgccacttt tcttaattta
    27061 ccaaactcta aaggcgtgta atatttttga acctttaaat gtttacgtga aggttgtaaa
    27121 tattgaccaa tcgttaaaat atctacatca ttcgcacgta aatcatccat cgtttcataa
    27181 atttcttcta tagtttcacc taatccaacc ataatacttg atttagttgg gatatccggt
    27241 tgtaattctt ttgaacgacg taaaaactct aatgttctgt cgtatgtcgc acgcgcacga
    27301 actctcggtg ttaatcgacg aacagtttca atattatggt ttaaaatgtc aggtcttgaa
    27361 gccattaatg tttctaacgc atcatagtcc ccgcccatat ctgatggtaa aatttcaatc
    27421 gttgtaaatg gatttctttc tctaacttta cgtactgtct cagcataaac atttgaacca
    27481 gcatctctta aatcatcacg cgcaacagca gtgataacaa cgtgtttcaa gttcattaat
    27541 tcaactgatt cagctacacg ttcaggctca tttaaatcaa gttcattagg taaacctgtc
    27601 ttaaccgcac aaaaacgaca agctcttgta catacggcac ctaaaatcat aaatgtcgct
    27661 gtacgacgtg caccccaaca ttcatgtata ttaggacatt tagcttcttc acatacagta
    27721 ttaagatttt tttccctcat catcttctta agtcctgtat agttttcgtt ggtatttaat
    27781 tttattttca accaatccgg tttacgtaat atttcctcgt tttttgtcgc cataacaaca
    27841 cataccctcc tgttaagtgt atctttaaac attatatcga aattcatgac aaatgaaaat
    27901 ccaataatct tactttttaa tattccaata atttttcttt aaatatatct cttaaaaact
    27961 ctggcattaa atactctttt ggtaattctt tcaatgttgg gaaataacga ccgaacgtat
    28021 acatatctat cgtacctaat acatattctt tgtcgtcttc gatttcttct ccattcagat
    28081 agtaacgccc tgtagaatga atgtacccta aattataaag aatatatcct ccaaatatat
    28141 tccctctgaa acccaaacct tgtgcatgtt catacatata ttcttgttta ttacttttag
    28201 ctaaaatctc ttttaatttc acaccagaaa gtctaaccct taccatatta atcggatgcg
    28261 gtaacatttg atgaatgtca tattcagtca cttcatcttt tacaatatct ttaacgagta
    28321 agccagcatt gatgatagca cattgagcat gtgtatactc acaaacactt tgagctaata
    28381 agtatgcagc ttcagttatg tggttcatac tacgcttaag cactactgga tgttgaatta
    28441 ctggattaga cattagcgtt tttccttctt cttcaaatga agttttaact tcaggtaatg
    28501 tttctaaagg aataatcttt gcagttttat gtactacttt atgtgcctca aaagttaaat
    28561 taacctctcc aagataatta ccatactttc cagctgccgc catcagtaca ccattattaa
    28621 tttcaccatg ttcaaaataa tgatgcgtat gactaccaaa aatgacatca atttcaggca
    28681 attcttggca taatgtttca tcgaagaaaa tgccacaatg acttagcacg attaatacat
    28741 caaatttacc tcgttgatgt tcaatttctt cttttataga atcaagtgga tcggtaacaa
    28801 tccaatctag tgcacgataa aatggggtaa aaggtgctgt cgctgcaacg aatagtattt
    28861 tcacaccgtc tatatcctta atataagaag aaacgatatt atttggtaaa tgacctgatt
    28921 catctataac attgctacat gtcactataa attttgcttc gtcgtaaagg tgatttaaag
    28981 cttcgtgtga aatggtcatc ccttcattat taccgattgt tgcaacatca cattttgctt
    29041 catttagtaa tgctacattc tttttaccta aagttgcttc tgttataggt gcggataaat
    29101 ctacatgatc acctagatca acatataaag aaggatgatt aagtcgtggc cgttgttctg
    29161 ccatatatgc tttaatgcgt tcgtattcat gtaaatgact atgaatatcg ttcgtatgat
    29221 aaattgtaag cctcaaattg acgtcctcct tttaaagttg tattaaatca acgcacgaat
    29281 aattaaatat atacccatta acaacattac tgttctcaat aatgttacta ccgtatctga
    29341 tttaattgat tgattcactt tgacaccgat tttcgcacca aaataactag atataattaa
    29401 aatgattgca taaccccaag ctacgtgacc ttgagcaatg tgccctatag aactcatgac
    29461 acttgaaaag aaaatcatca tcatacttgt tccaacagct acatgaggtg gaaatctaaa
    29521 tacaataagc attagtggcg tcattagtgc gccacctcca ataccaaata aacctgtcaa
    29581 tataccaatt aaaaacgttg taataaaagc aaacaatggt ggcacactat aatgatatgt
    29641 tttaccttta gcgtctacat aagttttttc atactttggt ttatcaaatg ttttaaacgg
    29701 tttaatctta tttcttacca ttaataaaat agctacaaag attaaaaaga ttccaaaata
    29761 taaattaaat gactcaaatg ttaaatatct actaatgaat gacccaagca atgaacctgg
    29821 taataatcca aataaaaaga tagagccatt tttaatatca acttgttttg tttttagata
    29881 tccaagcgat gaagaaagtc ctgtcacaat tagaatgaca gacgatgtcc caatcgctat
    29941 ttgcgttgta atattatgta gtaatccatg ttcaacaccg aggtaaacca ttgttggaac
    30001 gataataata ccgcctccaa tgcctacgat tgaccctata atcgctgaca aaccaccgat
    30061 tagaactaat aatgtaattg ttaataacat agtcgtcgcc tcttaaaata attttaattg
    30121 ttgtggtgct aacccttcat attctatatt caataatcgc tgatatgttt tggcattatt
    30181 tgctgcatga ccaccagaat tattgttaaa tatgacgtaa actttcttag ctttttgcgc
    30241 taatatttgt gccttttgtg ctaagtctat taattcttgc tcattataat catataaata
    30301 gcgtacatct cgccattctt gatctgacat atctttctta gtccaaccat aatgattacg
    30361 tccatgataa cgtacaaacg caatttcatt tgtgattcga ttgactaaag gtacactgcc
    30421 atctttcact tgtggttcat caactactgc atgaatgatc tgatgttctg ttaaaaatgc
    30481 caatgtttgt tctttaaatg catcactaaa ccatgattga tgcctaaatt ctacacacat
    30541 tggaaatgct tgtaattgct gtcttacata caaaatatat ttgatatttt gtgcattgca
    30601 gtcaaaccac ggaggaaatt gaactaatac cattgctaat tttttctgtg tatgtaaggg
    30661 ctctaacata tttttaaatt gatcaaatag ttcttgcctt gtatctgcaa atgttttgta
    30721 gtctgcatgc aatgtgagtg cttgatgaat tttgaccaca aattcaaatg tatcaggcgt
    30781 ttcttttatc catttcaata tatttctttc cggttgtatc gcatagtatg tcgcatctaa
    30841 ttcgacaacc ggaaaatgtc cagcatatgt tttaagttta tcggtttggc gttctaaatc
    30901 ttcatataat gaatagtgat caccccaacc tgttaatccg atgtttatca tatatatcac
    30961 caatgtcatc ataccatata acttttatca taatcatttc agcgaacttt agtttgatgt
    31021 tttcgttaga ttataatcat ttcagtgaac tttagctcga taatttcgtt tgatcataat
    31081 catttcagtg aactttagct tgatgatttc gtttgatcat aaacatttca gcgaacttta
    31141 gctcgataat ttcgtttgac tagattggta aaatagaaac gcaatccaca aaaatgacaa
    31201 gcactaaaat aatgtttggg ggtgcttgtg cttttgtgga ttgcggtcga ttatttatat
    31261 tgcatgattt gattaatttg attgattata ttggacatga tggtgttggc gggatgcgtt
    31321 gttgctagtc gcgggctttg tccactccac atatgtgtta actctttgtc gccgatgttt
    31381 gctgcggctt ttcttatgct acttgttagc tcattttgta ttggataatc tgggatgtcg
    31441 ccttcgtatt gggacatttc ttcgataaac ctattgttga taccgcgtgc aagctttcca
    31501 ctaaacgctt ttgtaacgac tgtatctgtt tctttactat ttataattgc atctcgcagt
    31561 agttctgatg cattactatc ttgtgatgtt aaaaatgcgg tgcccatttg taccccttct
    31621 gcacctaaga caatacttgc caaaactcct ctaccgtcca taattccacc agcggcaatg
    31681 accggaattg aaacgacatc tacaatttgt ggcactaaag atattgttcc aaccataggt
    31741 aattgatttt taggttttaa aaatgaacca cgatgtccac ctgcttcact accttgagca
    31801 acgatagcat ccatacctgc tttttcattc gcaatagctt catcaacact tgttgctgta
    31861 cctattagct taatgtttgc ttctttcaat ctttttataa tagattcatt tggaattcca
    31921 aaagtaaaac aacatacagg cacttgcttt ttaattatcg tatcaatatg acacttaaat
    31981 tgttgttctt cggtaatttt tacaaccggc tcttctaaat gtaatgcgcg tcgataaggt
    32041 tttaaccatg cattcatatt ttcaatttga ctactggtat atgattgttg acttggtaca
    32101 aagacattta caccaaaaga atttgacgtt aattggcgta cataatctat ttcgtcttct
    32161 aattgctgcg tattaaagta acctgcgcct attgtgccta atccaccact gttacttact
    32221 gatgcaacta atttcggtgt cgtacttcct gccatacctg cttgtataat tggatattca
    32281 atacttaaca tttgagtaag tcggttctta ttccacatag ctgttcgctc cttatataga
    32341 tacgttgcga tttttccgtt gttgaaattg aatttgctgt tgagaaagtt tttctttttc
    32401 ctttttatcc atctcatctt caatttccat acctaataat tcttcaatta agtcttcatg
    32461 tgacactatc gcttcagtac caccaaattc gtccaacaca attgctaaat gttttctaga
    32521 aatagtcatc ttacgtaata cccattcagc tttattgtgt tcattcacaa ataatggctt
    32581 agctgaatag tttgtaattt gatcttcttt tttattactc caagccaaca gatatttaga
    32641 atgaaacacc ccaataatgt tatcaatatc tccctcgtac actggatatc tagtgtatgg
    32701 cttattcata accgtttcat aaacttcttc gtatgtcgca tttgaagcaa atgccgtcac
    32761 attaattcta ggtgttgtat caacatcttt tacttttaaa ttttcaaaat taatgacacc
    32821 ttccaaccta ctcgtctcaa tttcatttaa agcaccttca tgtccagcaa ttgctaacat
    32881 tgttttaaat tcttcttttg aaaattgatg ttcttgaggt tgacccttag ataaacttcg
    32941 attaatactg tccgtcaact tatttaaaag taatgtgata ggacggaaca caatgacaca
    33001 aatatttata attggatata caagccttgt tattttatct ggaaatgtcg cagcgacaga
    33061 cttgggaatc acttcggaaa tcaaaatgat aacaactgtt aaaacagctg atgcaatccc
    33121 aacgctaatc ccccaacgta aagccataat tgtaacaagt gttggtaata aaatattcgc
    33181 gacattattc ccaattagaa tagttgtaat aaactcactt ggtttttcaa gtaactttac
    33241 aatgcctttt gcttttttat cacctttgtc agcttcagtt ttaaattttg ttttattggc
    33301 agccgttaat gccgtctcgc ttcctgaaaa gaaaaacgaa ataaatatca atataattat
    33361 ggcaatgatc acgcgtgtag tctccttatt gtcatatctt atttttattg tagttaccta
    33421 attcccgacg ttctcgttta ataaac
//
LOCUS       DATTQF010000017        32925 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.017, whole genome shotgun sequence.
ACCESSION   DATTQF010000017 DATTQF010000000
VERSION     DATTQF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.017"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(145..1050)
                     /locus_tag="VB241_000692"
     CDS             complement(145..1050)
                     /locus_tag="VB241_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1244015.1"
                     /translation="MTNQDNNHQLNHRIYQFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTNQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GVPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITKKEYNDALKYDIKSHLLNRKKR"
     gene            1431..2693
                     /locus_tag="VB241_000693"
     CDS             1431..2693
                     /locus_tag="VB241_000693"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP1244016.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(2787..4061)
                     /locus_tag="VB241_000694"
     CDS             complement(2787..4061)
                     /locus_tag="VB241_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP1244017.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDSEDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            4233..4850
                     /locus_tag="VB241_000695"
     CDS             4233..4850
                     /locus_tag="VB241_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP1244018.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLITGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            4978..6444
                     /locus_tag="VB241_000696"
     CDS             4978..6444
                     /locus_tag="VB241_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HEP1244019.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHAAPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAAALGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFVYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIVATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHNHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            6550..7680
                     /locus_tag="VB241_000697"
     CDS             6550..7680
                     /locus_tag="VB241_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1244020.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYRDEPVELKLQNISTNIADRFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDANLFSLKGALWTLAQEVYQEWYLGSKLYEEVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALNGNKRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLSGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(7641..7736)
                     /locus_tag="VB241_000698"
     CDS             complement(7641..7736)
                     /locus_tag="VB241_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244021.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(7830..9434)
                     /locus_tag="VB241_000699"
     CDS             complement(7830..9434)
                     /locus_tag="VB241_000699"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEP1244022.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(9421..10581)
                     /locus_tag="VB241_000700"
     CDS             complement(9421..10581)
                     /locus_tag="VB241_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEP1244023.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(10696..11142)
                     /locus_tag="VB241_000701"
     CDS             complement(10696..11142)
                     /locus_tag="VB241_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP1244024.1"
                     /translation="MHQHDFKVKTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            11222..11965
                     /locus_tag="VB241_000702"
     CDS             11222..11965
                     /locus_tag="VB241_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP1244025.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKFDKKLLIELKSPNLYPGIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRKYWYKKPNFSRIAQIASYVNPNYALVTRKFVDEAHHHQLQVMPY
                     TVNKLKTGEKLRQIGVDGLITDDPKLFIK"
     gene            complement(12181..12783)
                     /gene="rpsD"
                     /locus_tag="VB241_000703"
     CDS             complement(12181..12783)
                     /gene="rpsD"
                     /locus_tag="VB241_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP1244026.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPSQTISVREKSQKLNVIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(13027..13491)
                     /locus_tag="VB241_000704"
     CDS             complement(13027..13491)
                     /locus_tag="VB241_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP1244027.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFKGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            13628..15322
                     /gene="ezrA"
                     /locus_tag="VB241_000705"
     CDS             13628..15322
                     /gene="ezrA"
                     /locus_tag="VB241_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP1244028.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQCEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(15396..15531)
                     /locus_tag="VB241_000706"
                     /pseudo
     CDS             complement(15396..15531)
                     /locus_tag="VB241_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792848.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15681..16829
                     /locus_tag="VB241_000707"
     CDS             15681..16829
                     /locus_tag="VB241_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1244029.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     DQLLQQAKVQINDMVNTNMTYDVVFTSGATESNNIALKGVAYRKFDTAREIITSVLEH
                     PSVLEVVRYLEEREGFKVKYVDVTKDGTIDLQHFNDLMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNH
                     IQNIEPIIQGGGQEYGVRSGTVNLPNDIAMVKAMKMANENLEALNAFVTELNNDVRQF
                     LSQYQGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            16829..18052
                     /gene="thiI"
                     /locus_tag="VB241_000708"
     CDS             16829..18052
                     /gene="thiI"
                     /locus_tag="VB241_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP1244030.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITNRLSKIFGIKSISPVLKVEKSIEAMSAATIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGAYAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            18096..18868
                     /locus_tag="VB241_000709"
                     /pseudo
     CDS             18096..18868
                     /locus_tag="VB241_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
     gene            18966..19460
                     /gene="tpx"
                     /locus_tag="VB241_000710"
     CDS             18966..19460
                     /gene="tpx"
                     /locus_tag="VB241_000710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEP1244031.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            19583..20530
                     /locus_tag="VB241_000711"
     CDS             19583..20530
                     /locus_tag="VB241_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1244032.1"
                     /translation="MAEQQTIMERLFHSLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGTGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSRDFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKSVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            20617..21819
                     /locus_tag="VB241_000712"
     CDS             20617..21819
                     /locus_tag="VB241_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP1244033.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVDGEKVKTVQDIKDHVEAVDIMLDAFKKHNIINDINDIDGTGHRVVHGGEKFPESA
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLQRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTYGGLK"
     gene            complement(22066..22566)
                     /locus_tag="VB241_000713"
     CDS             complement(22066..22566)
                     /locus_tag="VB241_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP1244034.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            22707..23825
                     /gene="ald"
                     /locus_tag="VB241_000714"
     CDS             22707..23825
                     /gene="ald"
                     /locus_tag="VB241_000714"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEP1244035.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALINRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALHL"
     gene            23953..24057
                     /locus_tag="VB241_000715"
     CDS             23953..24057
                     /locus_tag="VB241_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244036.1"
                     /translation="MILIHYMLLMNIPAYVFLACLLHIAFRLGQKFMS"
     gene            complement(24135..25190)
                     /locus_tag="VB241_000716"
     CDS             complement(24135..25190)
                     /locus_tag="VB241_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP1244037.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEINKIRKAAELADKCIEIGVSYLKES
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSQEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            25365..26054
                     /locus_tag="VB241_000717"
     CDS             25365..26054
                     /locus_tag="VB241_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEP1244038.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTEATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(26361..26774)
                     /locus_tag="VB241_000718"
     CDS             complement(26361..26774)
                     /locus_tag="VB241_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP1244039.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            26979..28277
                     /locus_tag="VB241_000719"
     CDS             26979..28277
                     /locus_tag="VB241_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP1244040.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVIDFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANQ
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            28530..29471
                     /gene="pde2"
                     /locus_tag="VB241_000720"
     CDS             28530..29471
                     /gene="pde2"
                     /locus_tag="VB241_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HEP1244041.1"
                     /translation="MISTMNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLAKALRTKLN"
     gene            29492..32689
                     /locus_tag="VB241_000721"
     CDS             29492..32689
                     /locus_tag="VB241_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP1244042.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDTCIANNIKPIFGMTIYVTNGLNNIETVVLAKDNYGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKNVADEHRDIVRVFDSHEDTYLDHRSVLVQGIKHVWIQD
                     VCYQTRHDADTISALAAIRDNTKLDLIHDQEDFGAHFLTENEIHQLDVNPEYFTQADR
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRREKVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGTKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVEERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT        11205 a         4717 c         5977 g        11026 t
ORIGIN      
        1 aaactattga tttgtttaat agctttcctt tagagtattt aaaataaaga acagcagtga
       61 gaaattttct aattgaaaat aatcttactg ctgtttttaa tatttggatg gattattgtg
      121 gttacttaaa agagtgagca tcaattaacg cttttttcga tttaacaaat gtgatttaat
      181 atcatatttt aatgcgtcgt tgtattcttt tttagtgatt tgatcttcga ttaacatacg
      241 ctttaataca taatgttgtc tttgaatact atatttcaaa tctttatccg attttaatgt
      301 tccatctttt tcgtagggtg tatagccata tgggctttgc aacaaaccga taaggtatgc
      361 agattgtgca attgataaat cttttggtgg aacaccaaat aaactatatg aagcggatgc
      421 aattccagaa atattagcgc cattataatc tctaccgaag ggaactatat ttaaatacgt
      481 atatataatt tcatcttttg agagtaggtg ttctaatcta attgctaggc gaagttcatt
      541 tgcttttcta ctatatgttt tttcgttggt aagaacttga tttttaacaa gttgttgtgt
      601 aattgtgcta ccacctgaac tttgattagt attaaaaata tcttgtatca ttgctcttaa
      661 aatcgccttt ggtaagatgc catcatgttt ataaaataaa gtgtcttcag atgacgttaa
      721 tgctttaatg acatttggac ttgatgtttt aggtcctata atgagtgagt tttgagaatg
      781 gttatactca tataacaaat ttttgttatt atgatctaat aattcatcgc caggtatttg
      841 tcgaactttt tttattaaag catcatctga taatgagtcg gacgttttag ttaaatgatg
      901 aaaatataaa gacatcgcaa tcacagcgat agcaacgatg gcaatgaaaa tcataaatat
      961 aaaaacaatg acatgtttga tagctttata tatcttttca aattgatata tacgatgatt
     1021 caattgatga ttgttgtctt gattcgtcat ccttaacctc ctaaattacg tatagtatac
     1081 catatttaag caatttgact tttattttaa cgttaattat aattagggaa aatgcataaa
     1141 gaataggata aaaggacaag taatattatt tgaaatcatc agagagctag tggtcgctgt
     1201 gaactagtgt ttttaataat atgaatacac ctttataact aacatgtcct acgtttatta
     1261 atgaacgtat cattaataga gtgaacatta tcttatgatt atgtttaagt ttggtggtaa
     1321 cgtgcaatcg cgcccttaca tatttgttgt aagggttttt ttacgttcaa aaagctttaa
     1381 agtattttaa caagtattca ctaaataatt ataaatagga ggaaataata atgacgaatg
     1441 tattaattga agatttaaaa tggagaggtc ttatttatca acaaactgat gaacaaggta
     1501 ttgaagattt attaaataaa gaacaagtga cgttatactg cggtgctgat ccaacggcag
     1561 atagcttaca cattggtcac ttactaccat ttttaacatt aagaagattt caagaacatg
     1621 gacatcgtcc tatcgtttta attggcggtg gtacaggtat gattggtgat ccatcaggta
     1681 aatcagaaga acgtgtgcta caaacagaag aacaagtaga taaaaatatc gaaggtatta
     1741 gtaagcaaat gcataatatt tttgaatttg gaacagacca tggtgcagtg cttgttaata
     1801 atagagactg gttaggacaa atttcattaa ttagcttttt acgtgactat ggtaaacacg
     1861 tcggcgttaa ttacatgtta ggtaaagatt caatccaaag tcgtttagaa catggtattt
     1921 catatacaga attcacatac acgattttac aagctattga tttcggtcat ttgaatagag
     1981 aattgaattg taagattcaa gtaggtggat cagatcaatg gggtaatatc acaagtggta
     2041 ttgaattaat gcgtcgtatg tatggtcaaa cagacgcata cggtttaact attccgcttg
     2101 taactaaatc agatggtaag aaatttggta aatctgagtc aggtgctgtt tggttagatg
     2161 ctgaaaagac gagtccttat gaattttatc aattctggat taatcaatca gacgaagatg
     2221 taattaaatt cttaaaatac tttactttct taggaaaaga agaaattgat cgcttagaac
     2281 aatctaaaaa tgaagcacca catttacgtg aagctcaaaa aacattagct gaagaagtaa
     2341 ctaaatttat tcatggtgaa gatgcattaa atgatgcaat ccgtatttca caagcattat
     2401 ttagtggtga tttaaaatca ttatcagcga aagaattaaa agatggattt aaagatgtgc
     2461 ctcaagtgac attatcaaat gacacaacaa atatcgttga agtccttatt gaaacaggca
     2521 tttctccttc taaacgacaa gcgcgtgaag atgttaacaa tggtgcgatt tatattaatg
     2581 gtgagagaca acaagatgtt aattatgctt tagcaccaga agataaaatt gatggcgaat
     2641 ttacgattat tcgtcgcggt aagaaaaaat acttcatggt taactatcaa taaatataat
     2701 tgcatagcta aataaattag agcctactca ttacattctt aggaatgaat atgagtaggc
     2761 tcttaatgta cttttctgtc tataaattat ctaaagaaat ctctatcgcc ttggccaggt
     2821 gattgacgtt ctgatcggct ttgacgtttt ggttgttctt tttgttgttt taatttcact
     2881 ttaacttctt tagttttacc atcacggata acggtaacag tgactgattc accaggcttt
     2941 ttattttcat ataaatagct tcttaaatca acatcatctt taattttctt gccatcaatt
     3001 tctgtaataa tatcaccttt tttaagatca atatcactat cagcttttgc gacataaata
     3061 ccgtcttcgc tatcagtatg aagttgctcg cgctcttctt caggtatatc tttcaaatta
     3121 attaaaccaa taccaatcga agggcggtct attttaccat gttttacaag ttgttcaatt
     3181 gttactttaa cttcattact tggaatagca aacccaatac cttcaacttg tgtcgcagca
     3241 attttcattg agttaatacc aactaaatta ccattaatat ctactaatgc gccacctgag
     3301 ttacctgggt taatagcagc atctgtttga agaacgctga cttttgtatt accaccagtt
     3361 gtctctgcgt caatagtacg ttcacttgct gaaatgatac cagatgttac agagttagca
     3421 aattgtaatc ctaatgggtt acccattgcg aatacgctat cgccagtttg tacttttgaa
     3481 gagttggcga attgaatcgc tttaatacct tttgtatttt caattttaag tacagcaata
     3541 tcagttactg catctttacc aactaatttc gctttaactt gttttttatt atgtaattgg
     3601 actctaattt catttgcgcc atcaataaca tgattgtttg taacgatata agctgaattg
     3661 ttgtttattt gatagatgac acctgagcct acaccggctt cagatggttt agatgatttg
     3721 ccttttaata agtcgtctac acttgatgct ttttgcatat taataactcc aacaattgta
     3781 ggggagacag attttatcat ttcatgaacg gtaccgaatt tcttgctttg accgtctaat
     3841 tgattgccac ctttattatt tgttgtctgg acagttgaac catctttatt taaaattgta
     3901 ctatttaata ctttgcctat accaagtact agaagtgcac caatgattcc agcaatcaat
     3961 gcaacgatga ctgttttaaa ccatggaaat ttaggtcttc tgtatctagg tgtttggcta
     4021 tggtttgttg ttgaatgatc tgtatgatta aaatctgaca tacttaacct ccattatatg
     4081 atttatatat gctttaatta tgtctttttt ttataattat tgctagtaat acactataca
     4141 actttagatg tattttcaga ctatcttgtt ctagtaaaaa tatagtgata aaatctagtt
     4201 gttataaaca aataagttaa gtaggtggca caatgtattc agtgattagt aagattttga
     4261 atttcatttt agttaaaatg tcaaaatcat tatacgtaat cggtaaagat aatattccta
     4321 aggatagtaa atatgtcgta acttgtacgc atgaaagtta taacgaagtc attatgctag
     4381 gtatggcatt atatcctaat caaattcatt atatggctaa aaaagaatta ttcaaaaata
     4441 aatggattgg taaattttta acgtctttaa atgcatttcc tgtagatcgt gaaaatccag
     4501 gacctagtac tttaaaacgc ccaatcaatt tgttgaaaga taataagaca gttggtatct
     4561 tcccaacagg ttctcgcaca tcgcaagaag gtgcaccttt gaaacgtggt gcttcaacaa
     4621 ttgcaatgtt aagtaagtca ccaatattac ctgttgctta tgttggtcct actaagattc
     4681 atggtcttat aactggacaa gcatacataa atattggtaa accaatcgat attaacgatt
     4741 tacctaaaga tttaaaacga aacgagcgta tcgattacat tacaaaagag attgaaacac
     4801 gaacagcaaa gttacaacaa gaattgcatg aaattgtaaa aagtttataa aataacttga
     4861 ggtagaaata tagctggtta gtaagcctag gtgactataa cggagtatcg aagtagttat
     4921 tctagttaca ttagtaatct ttatatttct accttatttt aagaaggatg acattcaatg
     4981 tataaatttt tccaaaattt agggcgttcg ttaatgttac cagttgccat tttaccggct
     5041 gcagcaatta ttgccggaat tggtaatacg ttaaatgcac ttcatgcggc gccgaaaatc
     5101 gcaatgttct ttactacggt aggtactacg attttagagc aattaggtat tttatttgca
     5161 attggtgtag ctataggaat ggcaaagaaa aatgatggtg cagttgcgct tgcagctgcg
     5221 ttaggatatt ttttagttac agttgtatta tcaccaatga aattagcgcc tttattagga
     5281 atgaaagctt ctgaaataaa ttcagctttt gaaaagatga ataatggcaa tgtctttgtt
     5341 ggtattgtaa taggtttaat agcagcttat gcatacaata agtttagtga aacagaatta
     5401 ccattagcat tatcattttt tagtggtaaa cgtttagttc caattatgac tgcattttac
     5461 tgtacatttt tagttgtcat attgttattc ttatggccac tactttattc atggattgta
     5521 aaatttggtg aatctatagt tggtttagga tcatttgggg ctttcgtata tggtgttgcc
     5581 aacagattat taattcctac aggattacat catgcactga atagtgtgtt ctggtttgat
     5641 acgattggta ttaatgatat tggtaaattc caaagtggca aagatgctat aaaaggtatt
     5701 acaggacgct atcaagcagg attcttccca atcatgatgt tcggtattcc agcggctgcg
     5761 ttagcaatgt atcatacagc taaaactact cagaaaaaac aagtttacgg ttggttccta
     5821 gcgagttcag ttgcagcatt tttcgttggt gttacagagc cgattgagtt tgcatttatg
     5881 tttgttgcgc caatcttata tgttgttcat gcgttattaa caggattatc tttatttatc
     5941 gtagctacat tccattggac agctggattt tcatttagtg caggtttgat agattatgtg
     6001 ttatcactaa ttaatccggt atcaaatcat ccattgatgt tgcttgttca aggtgttgta
     6061 tttttcatct tatactatgt gattttccgt gtagtaatcc aagtatttaa cttgaatacg
     6121 attggtagag gtgaaaatga attagttgat ccaacagttg taaaagataa tattgctcct
     6181 ggtgaaaatg atattaaaca aagtaaatat cataatcatg ctatacaaat attagaaggt
     6241 ttaggtggtc aagagaatat tgttaattta accaattgtg caacaaggtt acgtctagag
     6301 ttaaaagaca catctattat tgatcaacag aagattaaaa acgccggtgc agttggtgtt
     6361 actgttaatg gcaaacattc aactcaagtt attgttggta cacacgttca gcaggttgct
     6421 gatgaaattg aaaaacattt ataaaattta gacagctaaa gcgcaaatcc aactcaatag
     6481 ggtttgcgct ttttttagtc aaatagtaga atgatttata tctgaatttt cagaaatgga
     6541 gtggggattg tgaagaaaat tttatttgat gttgatggcg tttttttaag tgaagaacgt
     6601 tgtttcgatg tctctgcatt aacggtatat gaattattaa tggataagtg ttatttaggt
     6661 cttcattcac atatagattg ggaaactttg actgataacg atatacaaga cattaggaat
     6721 cgaatttttc aaaaagataa gattttaaac aaattaaaat cgctagggct gaattcaaat
     6781 tgggatatgt tatttatcgt ttttagtatt catttaattg atattttgaa aaagttatca
     6841 catgatgaaa ttgaggcatt catgtatcga gatgagcctg tcgaattgaa gttgcaaaat
     6901 ataagcacaa acatagcaga ccgttttaat ttaaatgaac aactaccttt acaatttttg
     6961 gataatgtaa aagttggtaa aaataatatt tatgctgctt tggaagagtt tgcaacaaca
     7021 gagttacatg tttcggatgc taatttattt agtttgaagg gtgcattatg gacgttagcg
     7081 caagaagttt atcaagaatg gtatttagga tcgaagttgt atgaagaggt tgaaaagaaa
     7141 atagcacgaa ctacttttaa gacaggttat atttatcaag aaattatttt gagaccggta
     7201 gatgaagtta aggtacttct gaatgattta aaaggtgctg gtttcgaatt aggtatagca
     7261 acaggtcgtc cttatactga gactgttgtg ccatttgaaa acttaggatt gttaccgtat
     7321 tttgaagctg attttattgc aacagcaagt gatgttttag aagcagagaa tatgtatccg
     7381 caagcacgac cattaggaaa gccgaatcct tttagttata tcgcagcttt aaatggtaat
     7441 aaacgcgata aatatgaatc ttatatcaat aagcaagata acattgtaaa taaagatgac
     7501 gtatttatag taggcgattc gttagctgac ttattaagtg ctcaaaaaat aggtgcaacg
     7561 tttattggaa cattatcagg tttaaaagga aaggatgctg caggtgagtt agaagcgcat
     7621 catgccgact atgttattaa tcatttaggt gaacttagag gtgtactaga taatttgtaa
     7681 tttgattgtt gtttgacagc ataacttgaa gtgaatgatt gaaccaaagg tttcatattg
     7741 agttacaatg aaattaatat taaaaaaatg ccaagaagca atggaagtaa tccaatgtct
     7801 tcttggcatt ttgaatttat ataaattgtt tatgactgta ccgtcaattc agttgtgaaa
     7861 atttgattgt attcaccaac ttgtttaagt tcatcaatta tattgtttga aacaggttga
     7921 tcaacggata aaatcattag cgcatctccg cccgcttcag ttctacctaa agtcatagat
     7981 gcaatgttga tattgtattt acctaacaat gcgccagttt tacctaccat gcctggtgta
     8041 tcattatgat atgacacaat ttgatattga tttggcttta agtctacaga aaaattatta
     8101 attctaacaa ttcttggacc gaaacctgta aagacagaag caccaacttt aacggaatcg
     8161 ctatcgtttg ataattctac ctctaagtag ttactaaaac ctgtctctgc tttattattt
     8221 tcaatattta atgtcacttg ttgttgattt aacaacatta gagcattgat aatattgact
     8281 tcaggaccaa gatcatcttt taaaatatga gtaataattg tacgtgttaa taaactacta
     8341 tcaatagagg ctaagtcgcc accgtaggta attttaatag agcttggtgc attgtacatt
     8401 aattgaatag ctaattcacc aactgtttgg cttaaattga tgaatgattt tacagtatca
     8461 tcaatattgc ttaagtccat tttaggtgca ttcactgcat gcgttacagt accatcaatt
     8521 aaaatttcga tgatttcatt tgaaacagaa attgccactt tttcttgagc ttcgactgtt
     8581 gaagcaccca aatgaggtgt aacaataatt ttatcatgtg caacaagagg cgaatcagtt
     8641 gcaggttcat gttcaaacac atcgatagct gctcgactaa tttgtccttc gtctaatgct
     8701 tttattagtg ccttttcatc aataatacca ccacgtgcca cattgattat ttgcaaacta
     8761 ggttttgctt tggcaaaaaa gtcagcatta attaagcctt ttgttttagg tgttagtggt
     8821 gtatgcaatg taacgaaatc agaatgttgg gcaatctcat caactgttgc cttcgtaatg
     8881 cttaaagatt ttgctttttc atccgttaag taagggtcaa aagctagtat tttcataccg
     8941 aagctttgtg cacgtttagc aacacctaaa ccaattctac cagcaccaat gacacctaat
     9001 gttttatgat aaagctcagt acctttaaat gcatttcgat tccattcttt atttgtaagt
     9061 gattggtgtg cttgcggaat atttcgagcc attgataata acattgctag tgaatgttca
     9121 gtagctgaaa tcgtattacc atctggggca ttaataacta aaataccttt taatgttgca
     9181 gcatcaatat taatattatc tacaccaaca ccggcgcgtg cgattacttt taaagaatca
     9241 gcagcattta tgatattttc agtaactgta gtttgactac gaacgattaa agcatggtat
     9301 gaaggtataa ttttgattaa tgcttcttcg gacaaaccag tttgaatatc tacattgaat
     9361 tgttcgtgat ctaataatgc tttgatacca tcttttgata tgggatctgc aacgagtaca
     9421 ttaaattgct tcatgaataa cctccatata ttttgatatg cctttaccga tatagttaac
     9481 tttacggtgt tcagttaaaa taatttctaa agcagatact accgataaaa tatcgaaagg
     9541 actaattttc cccatatgac caattcttaa aatttgacct ttaagatggc cttgaccacc
     9601 agcaattgtt attttaaagc gatttttaag ttcatctttg attattttca cttcatcatt
     9661 tgtgttaggt ttgaatgccg taaccgttgg agatgcatct ttatcattga ctaataaagt
     9721 taaatctaat gcctttaagg cgcttcttaa tgcatttcta attgcatagt gtcgtgctat
     9781 tacgtgattg aaaccttctg cttttacggt ttcaacgtat gcatttacac ctctaaataa
     9841 agacacattt ggtgtgaacg gtgtagaatt gtcagcttgt gacgaaatgt atttatttaa
     9901 gtctagataa aattttggtg tagttacttc tttgaaacgt tcttttgcac ggtggctata
     9961 agctacaaaa gctaatcctg gaggtaacat aattgctttt tgactaccag aaacaagtac
    10021 atcaatttta tctttgttaa tgtcaacatc aacagcacca atgcaactta cgccgtcaac
    10081 tacaaaataa atattactat taaattgatt gatggcattt cctaactcgt gaatagggtg
    10141 tagcactgtc gtagatgttt cgcaatattg actaaatact gctttaactt caacatttaa
    10201 agttgaaagg taattgatga aatctttgac atctacagct tctccccatg ttacgtcata
    10261 aatatgtaca tttttgtaat aagtttgtgc aatttgttta aatcggttac caaaggcacc
    10321 tgaaacaatg acaacgaagt gatcttcagg gtttacaatg ttcaacatac tagcttctaa
    10381 gacgcttgta ccgctagatg ttaatataag tacatcattt tgactcccaa atattggctt
    10441 tagaccttga aatgcttgtt gagcgatgtc ttcaaaatct ttagaacgat gaccaaccat
    10501 aggtgcttga atttctctca taatggcatc aggaacaggg gttgggccag gtgttaataa
    10561 caacggttga tgataataca tttatatgcc tcctataaat aagatttcac tattttagca
    10621 aattttctga aaatttaaaa gcctaaaaaa agtgataacg ctatattgtc gaacaaatgt
    10681 tatcacttaa cgattttatt tggcctgaat gatgggatag atttttatgt ccacattatt
    10741 tcttacagca tttgaaatca tgcaattatt atctgcaatt gttatcaatt ttggtaatcg
    10801 cttttctaat tgtgctattt gatcacttgg aatttgaatg tgaggatggt gcacaatttt
    10861 tgacatactg aattttccat tatttaaaca agctgttcca atcgattgtt gttcaattga
    10921 aatatctgtg aactttgcac gttcaagagt agctgctaat gagatgatat aacatgatga
    10981 cgcggctgat actaacattt catcgggatt tgttcctata ccaacaccac ctaaagaagc
    11041 aggtattgaa atattctctg aaagtatgtc gccttgaacg tttccgacat tgttacgacc
    11101 accttgccaa gaagttttga ctttaaagtc atgttgatgc aattgcttta acctccaata
    11161 taattgtgat agtttaattt tagaatagtt tatcaaagtt taacagaaag gtgacttatt
    11221 aatgactctg aataaactga aagatgaatt acaaattgtt tcgcatcgtg gattgccgag
    11281 tgattttcct gaaaatacaa tggtcggtta tcgagaggta atggggctca atgttgctat
    11341 gttagaaata gatgttcatt tgaccaaaga ccaacatttt gttgtgatac atgatgaaac
    11401 aattgataga acatcggatg gtaaggggcg tattgctgat tacacattat cgcaattaaa
    11461 atcatttgat tttggtagtt ataaagatgt tgcttttaag ggagaacgaa tacccacgtt
    11521 agatgaagta ctttcattat gcttaaagtt tgataaaaag ctattgattg aattgaaaag
    11581 tccgaattta tatccaggaa tagaatgtaa attgctggca tttttggaag aaaagaaagt
    11641 cgatgcgaca caagtagtta tacaatcttt tgatatcgaa tgcatcgaaa agttaaacac
    11701 attaggcagt atatatgaat taggtgtgct ttgtagtaaa cgtaagtatt ggtataaaaa
    11761 gccaaacttt tcaagaattg ctcaaattgc tagttatgtt aatcctaatt atgcattagt
    11821 gaccagaaaa tttgttgatg aagcacatca tcatcaatta caggttatgc catacacagt
    11881 taataaattg aaaactggag aaaaattaag acaaataggt gtagatggat taattacaga
    11941 tgatccgaaa ttatttatta agtaagacga tgaatggtta tttgatagag ggataaggat
    12001 tcgtatatcg caattgatat ttttaaaaag aaatgatgaa atttgtagat aattcaacaa
    12061 ataagacttg taaattctaa gttaattgta aaatagggta gctcgatata atcttgaatc
    12121 tatcattttt aaattaataa aaaaaacacc cacaattgtg ggtgtttgta ttgaacggta
    12181 ttatcttgag tagtactcaa cgattaattg ttcgttaatt tcagcaggta attcgctacg
    12241 ttctggtaaa cgtacgaaag taccagttaa gctgtcagca tcaaagttta agtactcagg
    12301 tacgaaattg ttgatttcaa ctgattcaac gatgacgttt aatttttgag atttttcacg
    12361 aactgaaatt gtttgactag gtttaacaga ataagatgga atatcaacac gtttaccatc
    12421 tactaagata tgaccgtggt taactaattg acgtgcttga cgacgagtac gagctaaacc
    12481 taatgaataa acaacagcgt ctaaacgact tgctaataag atcatgaagt tttcaccgtg
    12541 tacaccgaat tttttaccag cgatgtcaaa tgtgttacgg aattgtcttt cagtcattcc
    12601 atataagtaa cgtaattttt gtttttcacg taattgtaaa ccatattctg ataatttttt
    12661 acgttggttt ggaccatgtt gtcctggtgc gtaaggacgt ttttctaatt ctttaccagt
    12721 accgcttaaa gagataccta aacgacgaga ttttttccag tttgaacctc tgaatcgagc
    12781 cataataaga ctcctccttt aattcttttt tgttgttatg aataaacaaa aaagagtgtt
    12841 gcatgcttac taagatatat tgttttatgt gtcttcacct tatagcgaca gttacgtgac
    12901 acgtccgcat tgggaacaac atagcgctca ataatataca actgctactt tcgttaattc
    12961 acacaaagtt tattgtaaca ttttcctgct aactgtcaac actttattga aaatgctgat
    13021 gtccttttat gcgagttgct tttcaataat tttaacaatt gcttcaagat gttccttgtc
    13081 attgtcatca aatcgatccg ttataggggc atcgatatct aagacaccga taattttatc
    13141 atctttaaaa attggaacga caatttcaga cttactatta gcatcacaag cgatatgtcc
    13201 tttgaattga tgaacatcag ctacaacttg tgtacgacgt tctgaaacgg ctgtaccaca
    13261 tacacctttt ccaattggaa tgtggacaca agcggggtgt ccttggaaag gtccaagtat
    13321 aagttcattt tgttccaata agtaaaagcc gacccaatta atttgatcta gattatcatt
    13381 taataaggca gacatgttac ttaaaatagc aatcatgtga tgttcatctt caataaggct
    13441 tgctgcttgt ttctttaata atgtgtagtt tgttgggtta attgttgtca tatttaaaac
    13501 ctcttatttt catagatttt ataatagtat agaatttcga aaatattatg gcaataaatt
    13561 cattttataa tttatcctta agcaaaatta cgttataata gtaatgataa attaggagga
    13621 gaagcatatg gtgttatata tcattttggc aataattgtg attatattga ttgctgtagg
    13681 tgtattattc tatttacgtt caaataaaag acaaataata gaaaaagcaa tcgaacgtaa
    13741 aaatgaaatt gaaacgttac cttttgatca aaaccttgca caattatcta agttgaattt
    13801 aaaaggtgaa acaaaaacga aatacgatgc aatgaaaaag gacaacgtag aaagtacaaa
    13861 taagtatcta gctcctgtgg aagaaaaaat ccataatgct gaggctttat tagataaatt
    13921 tagtttcaac gcatctcaat gtgaaattga tgatgcaaat gagttgatgg atagttacga
    13981 acaaagctat cagcaacaat tagaagatgt aaatgaaatt attgcgttat acaaagataa
    14041 tgatgaatta tatgacaaat gtaaggttga ttatcgtgaa atgaaacgtg atgttttagc
    14101 aaatcgtcat caatttggtg aggcagcaag tcttcttgaa actgaaattg aaaaattcga
    14161 gccaaggtta gagcaatatg aagtactaaa agctgatggt aattatgtac aagcgcacaa
    14221 ccatatagct gccttgaatg aacaaatgaa acagctaaga tcttatatgg aagaaatacc
    14281 agaattaatt agagaaactc aaaaagaatt acctggtcaa ttccaagatt taaaatatgg
    14341 ttgccgtgat cttaaagttg aagggtatga tctggatcac gtaaaagtag acagtacatt
    14401 acaaagctta aaaacagagc ttagtttcgt tgaaccatta attagtcgct tagaattaga
    14461 agaagctaat gataaactag ctaatatcaa tgataagtta gatgacatgt atgatttaat
    14521 tgaacatgaa gttaaagcta aaaatgatgt cgaagaaaca aaagatatca ttacggataa
    14581 cttattcaaa gcaaaagaca tgaattatac attgcaaaca gaaattgaat atgtacgtga
    14641 aaactactat ataaatgaat ctgatgctca gagtgttcgt caatttgaaa atgaaattca
    14701 aagtttaatt tctgtatatg atgatatttt aaaagaaatg tctaaatctg ctgtacgata
    14761 tagcgaggtt caggataatt tacaatattt agaagatcat gtcacagtta ttaatgacaa
    14821 acaagaaaag ctacaaaatc atctgattca attgcgtgaa gatgaagcag aagcagaaga
    14881 caatctgtta cgagtacaat cgaagaaaga agaagtgtat cgtcgattac ttgcttctaa
    14941 cttaacaagc gttcctgaaa ggtttatcat catgaaaaat gaaattgatc atgaagttcg
    15001 tgatgttaac gaacaattta gtgaacgtcc aatacacgtt aaacagttaa aagataaagt
    15061 gtctaaaatt gtgattcaaa tgaatacatt tgaagatgaa gcaaatgatg ttcttgttaa
    15121 tgctgtttat gcagagaaat taattcaata tggaaataga tatcgtaagg actatagcaa
    15181 tgttgataag agcttaaatg aagctgaacg attatttaaa aataatcgct ataagcgtgc
    15241 gattgaaatt gcagagcaag ctcttgaaag tgttgagcca ggtgtcacta aacatattga
    15301 agaagaagtt attaagcaat agaaaatagt atgtagttat acttaaataa tatgagctct
    15361 ctgtcaaatt ggactgatga gtttaataat tgaagttagc caacgatacg ttgtctagct
    15421 tcttttttta tatggatata tgaaagggac aataaatata aatagcaatt gtttaaagat
    15481 aaacgtaatc aaatgtgaag tttaaattaa tataagtagt gaaaaaagca tcgtgctatg
    15541 cattgagtga aatagttcaa ttcttattaa aatgggtgga aaaatgatta atttttgtta
    15601 tagcctttat tttttatgtg ataatagtat aatgcttgag taaaaacaat tttaaaagat
    15661 tgatgattaa tgaaagagaa gtgtttattt tgatatatct agataatgcg gcaacgacga
    15721 aagcatttga agaagtgtta gatacttatt taaaagtaaa tcaatcaatg tattataatc
    15781 cgaatagtcc gcataaagct ggtttgcaag cagatcaatt attacagcaa gcaaaagttc
    15841 aaataaatga tatggtcaat actaatatga catatgatgt tgttttcact agtggtgcaa
    15901 cggaatctaa caatatcgcg cttaaaggtg tggcttatcg taaatttgat acagcaagag
    15961 aaataattac atctgtttta gaacatccgt ctgttttaga agttgtcaga tatttggaag
    16021 agcgcgaagg ctttaaagtt aaatatgttg atgtaacaaa agatggcacg attgatttgc
    16081 aacattttaa cgatttaatg tccgataaag tcggtttagt aacatgtatg tatgtaaata
    16141 atgtaacagg tcaaattcaa cccattccac aaatggctga agttataaaa aattatccta
    16201 aggcacattt tcatgtagat gcggttcaag cattcggcaa aatttcaatg gatttcaata
    16261 acgtagatag tattagttta agtggccaca aatttaatgg gttaaaaggt caaggcgtct
    16321 tacttgttaa tcatattcaa aatattgagc caatcattca aggtggcggt caagagtatg
    16381 gtgttagaag tggtacagtc aatttaccca atgatattgc tatggttaaa gcgatgaaaa
    16441 tggctaatga aaaccttgaa gcattgaatg catttgttac tgagttaaat aatgacgtcc
    16501 gtcaattttt aagccaatat caaggtgttt atattaattc atcaacttca ggttcaccat
    16561 tcgttttaaa tattagtttt cctggcgtaa aaggtgaagt attagttaat gctttttcaa
    16621 aatatgacat tatgatatct acgacgagtg cttgttcatc taaacgtaat aaattaaatg
    16681 aagtattggc tgcaatggga ttatcagaca aatctattga aggtagtata agattatcat
    16741 ttggagctac tacaactaaa gaagatatag cgaggtttaa agaaatattt atcatcattt
    16801 atgaggaaat taaggagttg ctaaaataat gaagtatgat cacttgcttg ttagatacgg
    16861 ggagttaaca ttaaagggtt caaatagaaa aaaatttgta aatcaattaa gaaataatgt
    16921 aaataagtca ttaaaaggac ttgatggatt tgtcgttaaa ggcaaacgag atcgtatgta
    16981 tattgaactt gaagaccatg cagatataaa tgaaataaca aatcgattat caaaaatttt
    17041 cggtattaaa tctattagtc cagtattaaa agtagaaaaa tcaatagagg caatgagtgc
    17101 agcgacaatt aaatttgcgc agcaatttga agaaaacagc acttttaaaa ttgatgtgaa
    17161 gcgtgccgat aaaaatttcc caatggatac gtatgaatta caacgtgaat tgggtggtac
    17221 cgtattgaaa caaattgaaa atgtttctgt taatgtcaaa cgtccagatc atgaaattcg
    17281 agtggaagtt agattagatg caatttatat gtatgaagaa gttgttccgg gttcaggtgg
    17341 attaccagtt ggtactggtg gtaagacgtt actaatgctt tcaggcggta tagactcacc
    17401 agttgctggg atggaagtga tgagacgtgg cgtaacaatt gaagcgattc atttccatag
    17461 tccaccattt acaagtgatc aagcaaaaga aaaagttatt gaattgacac gtattttagc
    17521 tgaacgtgtt ggaccaatta aattgcatat tgtaccattt acagaattgc aaaaacaggt
    17581 aaataaagtt gtacatccaa gatatacaat gacttcaacg agacgtatga tgatgcgtgt
    17641 tgctgataaa ttagtacatc aaataggcgc ttatgccatc gttaatggtg aaaatttagg
    17701 tcaagttgcc agtcaaacac ttcatagtat gtatgcaatt aataatgtaa cttctacccc
    17761 tgtattacgt cctttattaa cttacgataa agaagaaatt attattaaat cgaaagaaat
    17821 tggtacattt gaaacatcta ttcaaccttt tgaagattgt tgcacaattt tcacccctaa
    17881 aaatccagta accgaaccaa actttgataa ggtagttcaa tatgaaagtg tctttgattt
    17941 tgaagagatg attaatcgtg ctgttgaaaa tattgaaaca cttgaaataa ctagtgatta
    18001 taaaactatt aaagaacagc aaacaaacca attaataaac gactttttat aaataaaatc
    18061 ttagagtgaa tttaaacata aggggatgtt aaactatgga tttgaattta acgatgatta
    18121 taatcataat tttatttggt tttatcgcgg cgtttataga ttcggttgta gggggggtgg
    18181 tggtttaatt tctacgccag cattattagc aatcggtcta ccaccatctg tggctttagg
    18241 tacaaataaa ttggcaagtt catttggttc tttaactagt gcgataaagt ttataaggtc
    18301 cggtaaagtg gacttatatg ttgttgccaa attatttggt tttgtatttt tggcatctgc
    18361 atgtggcgca tatattgcaa ccatggttcc gtcacaaata ttgaaacctt taatcattat
    18421 tgcactttcg tcggtgttta tattcacatt acttaaaaaa gattggggca atacacgtac
    18481 gtttactcaa tttacattta agaaagccat actatttgca gcacttttta ttttaatcgg
    18541 cttttatgat ggatttgtag gtggaggaac aggttcattt atgctttttg tattgttaat
    18601 ctttggtttt gattttttaa gcgctgcagg aaatgctaaa gttttgaatt ttgcttctaa
    18661 tataggtgcg cttgtattat ttatggtatt aggacaagta gattatgtaa taggtttagt
    18721 tatggctgcg agtatgattg tagggtcata tgctggggct cattttgcta tcaaacaagg
    18781 tgttggctat gtgaaagtat tatttattat tattactgcg attttaattt taaaaaatgc
    18841 ttttgattat attcaacaat ttgtataaaa ttaaattcgc ttttgattaa agcatgaaaa
    18901 ttgtaatcaa accataaatt gtcgtatgat gtagttagaa ttttaaaatg caggaggtca
    18961 agtatatgac tgaaataaca ttcaaaggtg gaccaatcca cttaaaaggt caacaaatta
    19021 atgaaggtga ttttgcacct gattttacag tgttagataa tgacttaaat caagtaacat
    19081 tagcagatta tgctggtaaa aagaaattaa ttagtgtggt accatcaatt gatacaggtg
    19141 tttgtgatca gcagactcgc aaattcaact ctgaagcttc taaagaagag gggattgtgc
    19201 ttacaatctc agcagactta ccattcgcac aaaaaagatg gtgcgcttca gcaggtttag
    19261 acaatgtcat tacattaagt gaccaccgtg acttatcatt tggtgaaaac tatggcgttg
    19321 ttatggaaga acttcgctta ttagctcgtg cagtatttgt attagatgca gataataaag
    19381 ttgtttataa agaaatcgtt agtgaaggta ctgatttccc agattttgat gctgctttag
    19441 ctgcatacaa aaatatttaa tcattaaaga gataaatctt aaaatgtata catcgtgtcc
    19501 atcgttgtca acagcattaa aatagaattg ttttctatga ttgctaagac ctatgggcac
    19561 tttttattgg agagggacga atatggcaga acaacaaaca attatggaac gcttgtttca
    19621 ttcattagat gaaaaagcta aaacattaaa taatgaaaat ggccaaagtt ttattgaaaa
    19681 tcttgggcta gcaatggaac aagtttatac aaatgaacga ggattgttag aacaatcgac
    19741 gttacaagat cgacgtaaag cattccaatt tgcatattta agtttaatgc aggaagaaaa
    19801 gatacaagca aatcatcaaa ttacaccaga ttcaattgga ttgatattag gatttttagt
    19861 tgagcgattt atgaacaatc aagaagaatt acatattgtt gatattgcaa gtggtaccgg
    19921 tcatttaagt gctactgtaa aagaagtgtt acctgaaatt gcggttatgc atcatttaat
    19981 tgaagttgat ccagttttat cacgtgttag tgtacattta gcaaacttct tagaaattcc
    20041 tttcgatgtg tatcctcaag atgccattat gccactaccg ttagaagaag cagatatcgt
    20101 tattggtgat tttccagtag gctattatcc aattgatgaa agaagcaggg actttaagct
    20161 aggtttcgaa gaaggacata gttattcaca ttatttatta atagaacaag caataaatgc
    20221 attaaaagat gctggatatg cctttctagt ggtaccaagt aatattttta caggtgaaca
    20281 tgtaaaacag cttgaaaaat atattgcaac agagacagaa atgcaagcat ttttaaattt
    20341 accaccaact ttatttaaaa atgaaaaagc gcgaaaatct atattaattt tacaaaagaa
    20401 aaaatcgggt gaaacaaagt cagttgaagt attattggca aatattcctg atttcaaaaa
    20461 tccttcacaa tttcaaggat ttatgacaga gttaaatcag tggatggaca caaatcgtcc
    20521 taaaaaataa tctgtaatag tatagtcata aaactgtatg aatgataaaa tgaaaatgaa
    20581 atagtataaa aagcaaacga tggaggatca ttacttatgt caaaattaat cttggctatc
    20641 aacgctggta gttcatcatt aaagtttcaa ttaatcagaa tgcctgaaga ggaattagta
    20701 acaaaaggat tagtagaaag aatcggatta aaagattcta tttttacaat tgaagtagac
    20761 ggggaaaaag ttaaaacagt acaagatatc aaggatcatg ttgaagcagt tgatatcatg
    20821 ttagacgcat ttaaaaaaca taatatcatt aatgatatta acgatatcga tggaactggt
    20881 caccgtgttg tacatggtgg tgaaaaattc cctgaatcag cagcaattac tgatgaagtt
    20941 gaaaaagaaa ttgaagaatt aagcgaatta gcaccgcttc acaacccagc taacttgatg
    21001 ggtattcgtg ctttccgtaa attattaccg aatatccctc atgtggcgat ttttgataca
    21061 gcattccatc aaacaatgcc tgaaaaagca tatctatata gcttgccata tcattattat
    21121 aaagattacg gtattcgtaa gtatggcttc catggtacaa gtcataaatt tgtgtcacaa
    21181 agagcggcag aaatgttaga taaaccaatt gaagatttac gcattatttc ttgtcatatt
    21241 ggtaacggtg catcaattgc agctatcgat ggcggtaaat ctattgatac atcaatgggc
    21301 ttcacaccac ttgctggtgt aacaatgggt actcgttctg gtaatattga ccctgcttta
    21361 attccattta ttatggaaaa aactggcaaa actgctgaac aagtattgga aattttaaac
    21421 aaagaatcag gtttattagg attatcaggt acatctagtg atttacgtga cttatcagaa
    21481 gaagctgaat caggtaaagc acgttcacaa atggctttag atgtatttgc ttctaaaatt
    21541 cacaaatata ttggatcata tgctgcgaga atgcatggtg ttgatgtaat cgtatttact
    21601 gcaggtattg gtgaaaattc agtggaaatt cgtgcaaaag ttcttgaagg tttagaattt
    21661 atgggcgttt actgggatcc taagaaaaat gaaaacttac aacgtggtaa agaaggattt
    21721 attaactatc cgcattcacc tgttaaagtt gtcgttatac cgacagacga agaaagtatg
    21781 attgctcgtg atgttatgac atacggtggt ctaaaataat tttttaataa atattaagcg
    21841 tctatctccg ctacaattat aagtaggagg tagacgcttt ttgtattgtt tgaaaattaa
    21901 ctagtgtaat gtcttttttc acgaatatct taaaaaaatt aagtagtcga agaagattat
    21961 gacaacatta ttaagtgagt tttatttata aacaataaaa aaccaagtgc agaaagttgc
    22021 acttggttca taatgatcaa gtagttatct aatgatcatc tatatttaat tttgatattt
    22081 ttcacgtaat tgagttgttg caacttgtgg ttggaagtct gctggtaact cttcagtacg
    22141 aacaactaac acgtcacatg gagcatgacg aacgatagat tctgatacag aaccaacaat
    22201 aaatctttcc acggcattta agcctgatgt accactcata atcaagtctg cattaatttc
    22261 atgtgcaagc tttttaggaa tgatagattt aggagagcca aactctagac gcgtttctac
    22321 atctttaaca ccagcgttag tagctacttc tttataacca tttaataatt cttctgcaaa
    22381 atgcttagat ttttcagtaa attgagcatc ataaacttca taagaagaat acgttcttga
    22441 atcaattaca ttcacaattg ttaacttcgc atcgttacgt ttagcaacac caactgctct
    22501 gttaaatgcc cattccgctt catgtgaacc gtcaactgcg attaaaatat ttttgtaagt
    22561 aatcatagta agtgcctcct tgttctttct taattttatt ttaccacaat tttcggaaaa
    22621 gtcagtgtaa taacctttac aattttgtgg atttttgatt gcgctttcaa aatgttcgcg
    22681 ttaagatgat tatggaggtg gatggtatga aaattggtat accaagggag attaaaaata
    22741 atgaaaatcg tgttggttta tcaccaagtg gtgtgcacgc tttagtagaa agtggacata
    22801 ctgttttagt ggaaacaaat gcgggttcag gatcgttctt tgaagatgta gattacaaag
    22861 aagcaggtgc tgagattgtt gctgaacaag caaaagtttg ggatgtggat atggttatta
    22921 aagttaaaga acctcttgaa tcagaatatc catattttaa agaaggacta gtattattta
    22981 cttatcttca tctagcgaat gaagaaaaat taacacaagc tttgataaat agaaaagtaa
    23041 ttagtattgc atatgagact gtgcagttac cagaccgatc tttaccatta ttatcaccaa
    23101 tgagtgaggt agcaggaaga atgtcagctc aagttggcgc agagttccta caaaaactta
    23161 atggtggtat gggaattcta ctaggtggcg ttccaggagt acctaaaggt aaagtaacta
    23221 ttatcggtgg tggtcaagca ggaacaaatg cagctaaaat tgcactagga ctaggtgcag
    23281 atgttacgat tttagatgtt aatccaaagc gtttacaaca attagatgat ttattcggtg
    23341 gacgtgtaca tacaattatg tcaaatccgt tgaatattga gttgtatgtt aaacaaagtg
    23401 atttagtaat aggtgcagtt ttaattccag gtgctaaagc gccaagactt gtaacagaag
    23461 acatgattaa acaaatgaaa aatgggtcag ttattattga cattgctatt gatcaaggcg
    23521 gtatttttga aacaactgat aaaattacga cacatgatga tcctacatat atcaaacatg
    23581 gtgtggttca ttatgcagtt gcaaatatgc cgggtgcagt accgcgtact tcgacgttag
    23641 ctttaaataa tgctacgcta ccttatgcgc tcatgctagc taataaaggg tatagagaag
    23701 catttaaatc aaatcaacca ttatcattag gtttaaatac ttacaaaggt cacgtaacca
    23761 ataaaggcgt tgcagaggca tttgaaatgg aatataaatc tgtagaagaa gcattacact
    23821 tataatttaa atttgatgaa aagggaatga attacagcgt attaaagaat gacgttatca
    23881 tctccttggt tattaaaatt aaataatgat aaattaaagc tagaaacaat gcgaagaaat
    23941 catcgaacat gaatgatatt gattcattac atgttactga tgaacatacc tgcatatgtg
    24001 tttctagctt gtttgttaca tattgcattt agacttggac aaaaatttat gtcctaggcg
    24061 ctacaatgtt atattggcag tgtttgactg aatgagaatg cgctagtaac aagcttttca
    24121 tttttgtccc actcttattt ttcgtaatgt gttaatactt cgtatccttc attagtgaca
    24181 agtatgtcat cttcaattct tacacctgca acacctggta cataaatacc tggttcgatt
    24241 gtaatgacca tgccagcttc taacaaatta gaattagtac ttgaaacatc ttgatattca
    24301 tgttcctgta atcctagacc atgacctaag cgatgaggga aatattcacc ataacctttt
    24361 tctgaaataa tatttctagc gatatgatcg atatctttta aaggtattcc aggtttaatt
    24421 gcttggattg cagatgtttc tgcttctaat acaatattat aaatttcttg tgcttcttgg
    24481 ctaggttcac caaatttaat ggtacgtgtc atatcgctac aataatgctc ataaattaca
    24541 cctaaatcaa atagtacata ctcattgctt tttaatctgc gatctcctgg tgtgccatga
    24601 ggtgatgcgg catgatctcc aaataaaacc atcgtatcaa aactcatttc attgacgcca
    24661 tattgtttga ttgtttgctc aatatggttg actacttcac gttcagttac actttctttt
    24721 aaataagaaa cacctatttc gatacactta tctgctaact cagcagcttt acgtattttg
    24781 ttaatttcat cttcagattt aatatttctc aattgtttga ttgttaaatc aacatctccg
    24841 aatgaattga cattgaaacc agagattaat tgtttttggc gtgctactgt taagtgctcg
    24901 ctttcaatta gtaatttatt gattgtttga ggataaagtg aaaaaggatt ttcagtgtct
    24961 aaatatccaa cgatttcacc tgtgaaaggt gatgctttga cttcttcgac ttccattttt
    25021 ggacaaaata gtacttgttt accatctttc ttaatcaata atgcaaataa tctttcatgg
    25081 ggttcgctac ggtatccagt aaaataatat acattcaacg gtgttgtaat ccatgctgca
    25141 tcagcttgtt gattgttcag ttcgtctatt atttttgata tttttgtcat tttataaaca
    25201 ctcctcaaca taatattact tacattttat gctaattatt attagatttc aaaatcgagc
    25261 taatacatta actagatttt caatttttaa aatttgagaa tttgttataa ttaagaggca
    25321 aaagggtact aaatttatat agcaattgat aggaggatgc tcaaatgaag ttatcatttc
    25381 acggtcaatc aacgatttat ttagaaggga acaacaaaaa ggttatcgta gatccgttta
    25441 tttcaaataa ccctaagtgt gatttgaata ttgaaacagt gcaggtagac tatatcgttt
    25501 taacgcacgg tcattttgat cattttggtg atgttgtaga actagcaaag aaaacagaag
    25561 caacagtaat tggaagtgca gaaatggctg actatctttc ttcatatcac ggtgttgaaa
    25621 atgttcatgg tatgaatatt ggtggtaaag caaactttga tttcggaagc gttaaatttg
    25681 ttcaagcatt tcatagttca agttttacac atgaaaatgg tattccagtt tatttaggta
    25741 tgccaatggg tatcgtgttt gaagttgaag gaaaaacaat ttatcatact ggtgatacag
    25801 gtctgtttag tgacatgtca cttattgcga aacgtcatcc agttgatgta tgttttgtac
    25861 caattggaga taattttacg atgggtattg atgatgcaag ttatgctatt aatgaattta
    25921 ttaaaccaaa aatttctgta ccgattcatt atgatacatt cccattaatc gaacaagatc
    25981 cacaacaatt taaagatgct gttaatgtag gggacgtgca aattttaaaa ccaggtgaat
    26041 ctgttcagtt ttaaatagaa tatagatttt acactaaaaa acacctcgct aatctatgtg
    26101 gaagttatcg tctgcatatt tttaatgggt gtttttatat tctactgatt cagttgcgac
    26161 gtggacgggg gagacatcag tggttcttta tcatttctga cctactctcg taattttagt
    26221 tttatacaat tagacattga ttataaattt actacgatat aaaaaatcca gaaccacagt
    26281 cataatcatt tcttgtgttt actcagtcgt ttgagactgc atcatgattg aaagattatt
    26341 tgtgattctg gataaaaatt ttatttaacg attaatacag ggatagtcgc acgttttgct
    26401 actttgtggc tgacacttcc aagtacaaat ttctttttgt cttctgcttt acggttactt
    26461 aaaacaacaa tttcgtattt accgctatta gcatgtttta atagttcttc ttttgcatta
    26521 ccacgaacaa ttatttggtc ataatcaata ccataatctt ctaaagcttt gcgtgttttt
    26581 tccaatcgct tgcttcgttc ttcagtaagt ttgtttaaat gaacacctgc tttaattgat
    26641 gcttgagcat cttgttcgct gattgcgttt aaaactgtta cgactgtacc ttcgccagct
    26701 aatttagaca cttcttttag tgctttttca ttttttaact gagtgtctac accaagtaat
    26761 atatttttat acattagtac atccctcctc acttacattc taataaataa taatggattt
    26821 ttccatttaa aattaaagat tttgtgatct ttgtgtttgt tgtatatttt caaaaaataa
    26881 aataggtgaa acgatcatta tttcatttca cttcaatgct ttttatccaa aataaagtta
    26941 taatgtagtt aatactgttc gaaaagaaga ggtaaagaat gacaaaacat gaacaaattt
    27001 tagattatat tgaatcgtta tctataggct ctaaaatttc ggtgcgtaaa atcgctaaat
    27061 ttttaaacgt atctgaaggg acagcatatc gtgcaattaa agatgctgat aaaatgggca
    27121 tggtcgcaac gatagatcgt gttggtacgg ttcgaatcga aaaacgtaat cgtaatgaaa
    27181 ttgaacattt aacttttaat gaaattgtca atattattga tggccaagtg ttaggtggca
    27241 acaaaggtat cactaagatg gtatctaaat ttgcaattgg agcaatggaa ctcaaagata
    27301 ttttaaagta tattgggcca aagacattat tgattgtcgg aaatcgcgaa gatgtgcaga
    27361 ttgaagcttt aaaaagaggc acggctatat taattactgg tggatttaag ccatctaata
    27421 aagttattga ttttgcgaat gaacatgatt tacctgttct atcatcaagt tatgatacat
    27481 tcctagttgc aaatattatc aacaaagcac tttttaatca gaaaatacga aaagatatct
    27541 taatagttca agatataatg acgccattag atgatttatc tgtgctgttt gatacgatga
    27601 aaatagcaga ttataagcgt atggcgaatc aaactggcca tacaagattt ccggtagtca
    27661 atgagtctta taaattagta ggtattgtaa caagtcgaga gatgattaat acgaaggacg
    27721 acgacgaaat agataaagtg atgacgcgaa atcctattta tgtaaatgct atgagtacag
    27781 tagcaagttg tgcgcatatg atgatttggg aaggtattga attgattcct gtcgtttcga
    27841 gtaataaaaa gacagtgggt gtgattaata gacaagatgt tttgaagtct atgcaacttt
    27901 taggaagaca acctcaaatg ggtgaaacaa ttaacgatca aatcgccaaa tatataacaa
    27961 tgaatcaaga tggaattaca gtagaagttt caccactgtt aatcaatcac tacggaacag
    28021 taagtaaagc agcatttgta agtattatag aagaaacaat acaatatgaa atgcgtaaat
    28081 ttaaaaaagg taatgttatg atagagaact taaatatcgt gtatattaaa acagtaccta
    28141 ttgaatcaca tataacagtg cgctttggca tactagacgt cggaagaaac ttcgccaaaa
    28201 tagaagtgaa catgcatagt caaaacgaca aagtagcgag cgctctcgtg atatgccaaa
    28261 tgtttgacga agtataggaa cttagcagag aaaataggca tacgatagat ttcacacaaa
    28321 tgttggagcc caacttgcat tgtctgtaga atttcttttt gaaattctct gtgttggggc
    28381 ccctgaacta attagtatga aaaaatatat atctaaataa ttaaatcggg aaaatagttt
    28441 aatatggtag aacaattttt ataatttaga tattttgata tagactaatg cttaaattaa
    28501 gcaatttttc taaaaaaggg agaatgaaaa tgattagtac gatgaatgaa attatgaaat
    28561 gtatcgaaga caacgataca attattatac acagacatgt tagaccagat cctgatgcat
    28621 atggttcaca acttggttta aaatactata ttcagcagaa atttccgcaa aagcaagttt
    28681 ttgcagtggg tgaagcggaa tcatcattaa gttttattgg agagttagat aacattgatg
    28741 ataaaacata tcaagatgcg cttgtaatag tatgtgatac tgccaatgct ccacgaattg
    28801 acgatgaacg ttatagtaca ggtcgtaaac ttattaaaat agatcatcat cctgcagttg
    28861 atcagtatgg tgatattaat ctagttaata cgaacgcgtc atctacaagt gaaatcattt
    28921 acgatttaat ctcacatttt aatgatgaag caattgttaa taaagatatc gcgagtgttt
    28981 tataccttgg tatcgtcggt gatactgggc gattcctttt taacaatacc tcagaacata
    29041 ctatggaaat tgctggaaag ttaattgggc atgatattga tcataatgcg cttttaaata
    29101 aaatgatgga aaaggatcca aaaatgttgc cgtttcaagg ttatgtttta caacatttcg
    29161 aacttatgga tgacggattt tgccaagtta aaataactga agatgtattg gagcaattcg
    29221 gtattcagcc aaatgaagca tctcagtttg ttaatacaat tgctgacatc aaaggtttga
    29281 aaatatgggt gtttgcagtt gatgagggta gtgaaataag atgtcgctta cgctctaaag
    29341 ggcaattgat tattaatgat attgcgcaag attttggtgg cggtggtcat ccgaatgcgt
    29401 caggagtttc agtgaacagc tgggatgaat ttgagcaact tgctaaagct ttacgcacga
    29461 aacttaacta atagaaaggg gccattcaat catggtggca tatttaaata ttcatacggc
    29521 ttatgatttg ttaaattcaa gcttaaaaat agaagatgcc gtaagacttg ctgtgtctga
    29581 aaatgttgat gcacttgcca taactgacac caatgtattg tatggttttc ctaaatttta
    29641 tgatacatgt attgccaata atataaagcc gatttttggt atgacaatat acgtgacaaa
    29701 tggattaaac aatattgaaa ccgttgtttt agcaaaagat aattatggtc tgaaggattt
    29761 atatcaacta tcatcggaaa taaaaatgaa tgcattagaa catgtgtctt ttgaattatt
    29821 aaaacggttt tctaacaata tgattatcat ttttaaaaat gttgcagacg aacatcgtga
    29881 cattgttcga gtttttgatt cgcatgaaga tacgtattta gatcatcgaa gtgttttagt
    29941 tcagggtata aagcacgtat ggattcaaga tgtatgttat caaacacgac atgatgcaga
    30001 tacaatttct gcactcgcag cgataagaga taatacaaaa ttagacttaa tacatgatca
    30061 agaagatttt ggtgcgcatt ttttaactga aaatgaaatt caccaacttg atgtgaatcc
    30121 agaatatttc acacaagctg acaggatagc tcaaaagtgt aatgcagaat taaaatatca
    30181 tcaatctcta cttcctcaat atcagacacc taatgatgaa tcagctaaaa aatatttgtg
    30241 gcgtgtctta gttacacaat tgaaaaaatt agaacttaat tatgacgtct atttagagcg
    30301 attgaaatat gagtataaag ttattactaa tatgggtttt gaagattatt tcttaatagt
    30361 aagtgattta atccattatg cgaaaacgaa tgatgtgatg gtaggaccag gtcgtggttc
    30421 atctgcaggg tctctggtca gttatttatt gggaattaca acgattgatc cgattaaatt
    30481 caatctatta tttgaacgtt ttttaaaccc agaacgtgta acaatgcctg atatagatat
    30541 tgactttgaa gatacacgcc gagaaaaggt cattcagtac gtccaagaaa aatatggcga
    30601 gctacatgta tctggaattg tgactttcgg tcatctgctt gcaagagcgg ttgctagaga
    30661 tgttggacga attatggggt ttgatgaagt tacattaaat gaaatttcaa gtttaatccc
    30721 acataaatta ggaattacac ttgatgaagc atatcaaatt gacgatttta aaaagtttgt
    30781 acatcgaaac catcgacatg aacgctggtt cagtatttgt aaaaagttag aaggtttacc
    30841 aagacataca tctacacatg cggcaggaat tattattaat gaccatccat tatatgaata
    30901 tgccccttta acgaaagggg atacaggatt attaacgcaa tggacaatga ctgaagccga
    30961 acgtattggt ttattaaaaa tagattttct agggttacga aatttatcaa ttatccatca
    31021 aattttgact caagtcaaaa aagatttagg tattaatatt gatattgaaa aaattccatt
    31081 tgatgatcaa aaagtgtttg aattgttgtc gcaaggagat acgactggca tatttcaatt
    31141 agagtctgac ggtgtaagaa gtgtattaaa aaaattaaag ccggaacact ttgaggatat
    31201 tgttgctgta acttctttgt atagaccagg tccaatggaa gaaattccaa cttacattac
    31261 aagaagacat gatccaagca aagttcaata tttacatccc catttagaac ctatattaaa
    31321 aaatacttac ggtgttatta tttatcaaga acaaattatg caaatagcga gcacttttgc
    31381 aaacttcagt tatggtgaag cggatatttt aagaagagca atgagtaaaa aaaatagagc
    31441 tgttcttgaa agtgagcgtc aacattttat agaaggtaca aagcaaaatg gttatcacga
    31501 agacattagt aagcaaatat ttgatttgat tctgaaattt gctgattatg gttttcctag
    31561 agcacatgct gtcagctatt ctaaaattgc atacattatg agctttttaa aagtccatta
    31621 tccaaattat ttttacgcaa atattttaag taatgttatt ggaagtgaga agaaaactgc
    31681 tcaaatgata gaagaagcaa aaaaacaagg tatcactata ttgccaccga acattaacga
    31741 aagtcattgg ttttataaac cttcccaaga aggcatttat ttatcaattg gtacaattaa
    31801 aggtgtaggt tatcaaagtg tgaaagtgat tgttgaagaa cgttatcaga acggcaaatt
    31861 taaagatttc tttgattttg ctagacgtat accgaagaga gtcaaaacga gaaagttact
    31921 tgaagcactg attttagtgg gagcgtttga tgcttttggt aaaacacgtt caacgttgtt
    31981 gcaagctatt gatcaagtgt tggatggtga tttaaacatt gaacaagatg gttttttatt
    32041 tgatatttta acgccaaaac agatgtatga agataaagaa gaattgcctg atgcacttat
    32101 tagtcagtat gaaaaagaat atttaggatt ttatgtttcg caacacccag tagataagaa
    32161 gtttgttgcc aaacaatatt taacgatatt taaattgagt aacgcgcaga ataataagcc
    32221 tatattagta cagtttgata aagttaaaca aatccgaact aaaaatggtc aaaatatggc
    32281 attcgtcaca ttaaatgatg gcatcgaaac tttagatggt gtgattttcc ctaatcagtt
    32341 taaaaagtac gaagagttgt tatcacataa tgacttattt atagttagcg ggaaatttga
    32401 ccttagaaag caacaacgtc aattaattat aaatgagatt cagacattag ccacttttga
    32461 agaacaaaaa ttagcatttg ccaaacaaat tataattaga aataaatcac aaatagatat
    32521 gtttgaagag atgattaaag ctacgaaaga gaatgctaat gatgttgtgt tatcctttta
    32581 tgatgaaacg attaaacaaa tgactacttt aggctatatt aatcaaaaag atagtatgtt
    32641 taataatttt atacaatcct ttaaccctag tgatattagg cttatataac ttttatcaac
    32701 taataaatta tgatatagta aactgatggt tagatatttt ttaaccatca gtttacgttt
    32761 atattagttt tttatgctta ttatttttat gagtttcact ttacattatt gattaatcca
    32821 taagaataat tagcacaaaa agcagtatac ataaattgag taaagaattt tgtcgatata
    32881 tcgtgagaaa aaataataac tttttgatat attggttttg ctatc
//
LOCUS       DATTQF010000018        32383 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.018, whole genome shotgun sequence.
ACCESSION   DATTQF010000018 DATTQF010000000
VERSION     DATTQF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.018"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(139..1152)
                     /locus_tag="VB241_000722"
     CDS             complement(139..1152)
                     /locus_tag="VB241_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP1244043.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQLNEK
                     NTL"
     gene            1462..2604
                     /locus_tag="VB241_000723"
     CDS             1462..2604
                     /locus_tag="VB241_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1244044.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2605..3723
                     /gene="mnmA"
                     /locus_tag="VB241_000724"
     CDS             2605..3723
                     /gene="mnmA"
                     /locus_tag="VB241_000724"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP1244045.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            4405..5073
                     /locus_tag="VB241_000725"
     CDS             4405..5073
                     /locus_tag="VB241_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP1244046.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQESIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNENIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            5075..7552
                     /locus_tag="VB241_000726"
     CDS             5075..7552
                     /locus_tag="VB241_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP1244047.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDSEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEDMLENTATNDTGASQTTIDDQVEAPMSSNASEEVTSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            7895..10525
                     /gene="alaS"
                     /locus_tag="VB241_000727"
     CDS             7895..10525
                     /gene="alaS"
                     /locus_tag="VB241_000727"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP1244048.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIVSAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDNQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            10588..10848
                     /locus_tag="VB241_000728"
     CDS             10588..10848
                     /locus_tag="VB241_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP1244049.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            10852..11280
                     /gene="ruvX"
                     /locus_tag="VB241_000729"
     CDS             10852..11280
                     /gene="ruvX"
                     /locus_tag="VB241_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP1244050.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            11295..11603
                     /locus_tag="VB241_000730"
     CDS             11295..11603
                     /locus_tag="VB241_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP1244051.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            11888..12526
                     /locus_tag="VB241_000731"
     CDS             11888..12526
                     /locus_tag="VB241_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP1244052.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLVTHHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            12529..13452
                     /locus_tag="VB241_000732"
     CDS             12529..13452
                     /locus_tag="VB241_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP1244053.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            13464..14732
                     /locus_tag="VB241_000733"
     CDS             13464..14732
                     /locus_tag="VB241_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP1244054.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            14732..15355
                     /gene="udk"
                     /locus_tag="VB241_000734"
     CDS             14732..15355
                     /gene="udk"
                     /locus_tag="VB241_000734"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP1244055.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            15383..15859
                     /gene="greA"
                     /locus_tag="VB241_000735"
     CDS             15383..15859
                     /gene="greA"
                     /locus_tag="VB241_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP1244056.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            16185..16919
                     /gene="pxpB"
                     /locus_tag="VB241_000736"
     CDS             16185..16919
                     /gene="pxpB"
                     /locus_tag="VB241_000736"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEP1244057.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITPSKLIENLELNKFNEKNVHAVNQTNSIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            16909..17919
                     /locus_tag="VB241_000737"
     CDS             16909..17919
                     /locus_tag="VB241_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEP1244058.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHTINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            17921..18370
                     /locus_tag="VB241_000738"
     CDS             17921..18370
                     /locus_tag="VB241_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP1244059.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKVNDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            18384..19745
                     /locus_tag="VB241_000739"
     CDS             18384..19745
                     /locus_tag="VB241_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP1244060.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNDAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDNAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQNDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLRATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            19745..20497
                     /gene="pxpA"
                     /locus_tag="VB241_000740"
     CDS             19745..20497
                     /gene="pxpA"
                     /locus_tag="VB241_000740"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEP1244061.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNEEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            20509..21741
                     /locus_tag="VB241_000741"
     CDS             20509..21741
                     /locus_tag="VB241_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEP1244062.1"
                     /translation="MGRNLKLKHRESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQT
                     AVFTSQFLASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISIL
                     IAFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMI
                     LGIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAH
                     RILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVF
                     EHAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLKERSNLIVIVFIVIST
                     MIFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAV
                     LVTILTGIFSFKEVLQLF"
     gene            22322..23074
                     /locus_tag="VB241_000742"
                     /pseudo
     CDS             22322..23074
                     /locus_tag="VB241_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            complement(23276..23545)
                     /locus_tag="VB241_000743"
     CDS             complement(23276..23545)
                     /locus_tag="VB241_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244063.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            23859..24545
                     /gene="mtnN"
                     /locus_tag="VB241_000744"
     CDS             23859..24545
                     /gene="mtnN"
                     /locus_tag="VB241_000744"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP1244064.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQVPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            24565..25092
                     /locus_tag="VB241_000745"
     CDS             24565..25092
                     /locus_tag="VB241_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP1244065.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            25093..26193
                     /gene="yqeH"
                     /locus_tag="VB241_000746"
     CDS             25093..26193
                     /gene="yqeH"
                     /locus_tag="VB241_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP1244066.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFGDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYSLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLI
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNLQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            26207..27013
                     /gene="aroE"
                     /locus_tag="VB241_000747"
     CDS             26207..27013
                     /gene="aroE"
                     /locus_tag="VB241_000747"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP1244067.1"
                     /translation="MKFAVIGNPISHSLSPVMYRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            27017..27307
                     /gene="yhbY"
                     /locus_tag="VB241_000748"
     CDS             27017..27307
                     /gene="yhbY"
                     /locus_tag="VB241_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP1244068.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKENKEIELP"
     gene            27310..27879
                     /gene="nadD"
                     /locus_tag="VB241_000749"
     CDS             27310..27879
                     /gene="nadD"
                     /locus_tag="VB241_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEP1244069.1"
                     /translation="MKRIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMVIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENGMIAIQIPRVDISSTMIR
                     QRVSKGKSIQVLVPKSVENYIKGEGLYEH"
     gene            27869..28453
                     /gene="yqeK"
                     /locus_tag="VB241_000750"
     CDS             27869..28453
                     /gene="yqeK"
                     /locus_tag="VB241_000750"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP1244070.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            28454..28807
                     /gene="rsfS"
                     /locus_tag="VB241_000751"
     CDS             28454..28807
                     /gene="rsfS"
                     /locus_tag="VB241_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP1244071.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            28810..29526
                     /locus_tag="VB241_000752"
     CDS             28810..29526
                     /locus_tag="VB241_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1244072.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYRHLADSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLTQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            29566..30252
                     /locus_tag="VB241_000753"
     CDS             29566..30252
                     /locus_tag="VB241_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEP1244073.1"
                     /translation="MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSKNKGPVYVDIKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLNSYFTT"
     gene            30344..30805
                     /locus_tag="VB241_000754"
     CDS             30344..30805
                     /locus_tag="VB241_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEP1244074.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAEYLTKG"
     gene            30810..>32383
                     /locus_tag="VB241_000755"
     CDS             30810..>32383
                     /locus_tag="VB241_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEP1244075.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYIAYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITNQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGIN"
BASE COUNT        11667 a         4364 c         6214 g        10138 t
ORIGIN      
        1 cattagaatt ttaagtgaat ttgaatcatt actaaaataa ataattgaac aataaagaca
       61 acttttattc aaaatgctgt tactgtttat ttttattaat attatggata catactttca
      121 cttagtttat ttatcacatt ataaagtgtt tttttcattt aactgattta taacattttt
      181 gaatcgacga taagagtcta attgcatatc tttatcataa atataactta tagccataat
      241 ttcgttgact tcaccataag tttccataaa atcttgaatt ttttgttgaa cagtattttc
      301 tgaacctatt aatgatattt tctgtctttc catagccatc gcatattctc tcggcgttaa
      361 tatattttgc agttcatgtg ttggcggttg aacgggctgc attctgccac gagtgatact
      421 aaccattact tgtgccatag atgaagctaa atattgtgct tcgtcatcat tttcagcaac
      481 gattgtattt aaacatacaa taacataagg ttcgtctaat acatcagaag gttcaaataa
      541 cgttttgtag atttcgatag cttctttcat ttgttgaggt gcaaaatgtc cagcgaacac
      601 atatggaagc cctttgcgag cagctaaatg tgcagaatct gttgaagaac caagaatata
      661 taaaggcaca tttttaccta ctgctggata agcacgaaca tatgcttgtt ggtgagcggg
      721 gccgaaatat tgttgtaata atgaaacttc ttccggaaat ttataaacac catcatgttg
      781 atctcgtctt aatgcacttg cggtcatcat atctgttcca ggtgcacgtc ctaatcctaa
      841 atcgacacga tttggaaata atgttgccat cgtgccaaat tgttccgcaa cgattaatgg
      901 agcatggtta ggtaacatga ttcctccaga acctacacgt atgtgtttcg tatgttctaa
      961 cgtatgttga attaataaag cagttgctga acttactagg ttgggagcgt tatggtgttc
     1021 agcaatccaa tatctttcat atgataattc gtctaaatgt tgtgcgagct taaccatatc
     1081 attaattgct gttcgttcat cttcaccttc tcgaataggg acaaggttta atgctgaata
     1141 tttaatcgcc atttttatat cgtccttcct ttttttgata taagaagtat accaatcgaa
     1201 taaccagata aatattaaat tgctcataac caaaataata gtgcgttaca ttttacaaat
     1261 gataaaatat cttcaaatac taaaatttat aaaaaagaca agaataaagc gataaactta
     1321 aaaagcctat cgaaaagcat gtgtagaaaa tggtaaaata taattaaaaa cattataaat
     1381 aaagttaaac agaaaagtag aaaaatagta tgtaaggtgc tattattgga acattgataa
     1441 aattgtgagg agtgtccaaa catggaaatt tatgcagatt atgctgcgac cacaccagta
     1501 aaacctgaag tagtagatgc gatgatgacg atttacaatt cgcattatgg taatccatca
     1561 tcaatacatg ctaagggaag agatgcgcgt aaatatttag atgagtcaag acgtcaaatt
     1621 gcacaattac ttggtgcaga tacacatgaa attatattta cgagtggcgc aacagaatcg
     1681 aataatacgg caattaaagg catagtaaaa gcaaatgaac aattgggtaa tcacattatc
     1741 acatcgaaga ttgaacatca ttctgtgtta catgtttttg aacaattaga gcgagaaggt
     1801 tttgatgtaa catatctaga tgttgatgat acaggtgcta ttgatttaga tcaacttgaa
     1861 gaaacaatta cagataagac gattttagta tctattatgt ttgtaaataa tgaagttggc
     1921 acagtgcaac aaatttatga tattcaagat attattgctg agacaaatgc atatttccat
     1981 gttgatgcgg ttcaagcaat tggacatctt gatgttaagt ttgacgaatt tgagatcgac
     2041 gcaatgagta ttactgctca taagtttggc ggaccaaaag gtgtcggtgc attattagtt
     2101 aaagaccatg tcacattaga ttatcctcaa cttggcggtg aacaagaatt gaaacgccgt
     2161 gcaggcactg aaaatcttgc acaaattgtt gggatggcta aagcattaca attagcagaa
     2221 aagaatcgag acgataacaa tattcattta atgaatttaa aagaacaatt tttagttaaa
     2281 ttacaagaaa gagcgattcc atttgaatta aatggttcaa tgacggatgc tacaggccat
     2341 attgtaaatc tatattttcc gtttgttgaa gttgaaacga tgttaacatt gttggatatg
     2401 gcacaaatct atgtgtcgtc tggatctgca tgtacagcag gctctacaca accgtcgcat
     2461 gttttagatg caatgttcga agatgaagag cgatcaaatc attcgattcg atttagtttt
     2521 aacgaattga ctactgaaaa tgaaattaat gcaattgtag ctgaaattca taaaatatat
     2581 tttaaattta aggaggagtc ataattgtca aataaagata tacgtgttgt cgttggtatg
     2641 tcaggcggtg tagatagttc tgtaacagcc cacgtcttaa aagaacaagg ttatgatgtc
     2701 attggcatat ttatgaaaaa ctgggatgac actgacgaaa atggcgtatg tactgcaact
     2761 gaagattaca acgatgttat tgaagtgtgt aatcaaattg gcattccata ttacgctgtt
     2821 aattttgaaa aagaatattg ggataaagtc tttacgtatt tcttagatga atataaaaaa
     2881 ggtcgtactc caaacccaga cgttatgtgt aataaagaaa ttaagtttaa agccttttta
     2941 gatcatgcga tgaatttagg tgcagattat gtagcaacag gacattacgc acgcatacat
     3001 cgtcatgaag atggtcatgt tgaaatgtta cgtggtgtag ataataataa agatcaaaca
     3061 tacttcttga atcaattatc tcaacaacaa ctttcaaaag tgatgttccc aattggcgac
     3121 atcgaaaaga gtgaagtgcg tcgaattgct gaagaacaag gacttgttac tgctaagaaa
     3181 aaagattcta caggcatttg ttttatcggc gaaaaaaact ttaaaacatt tttatctcaa
     3241 tatttacctg cgcaaccggg tgatatgatt acacttgatg gtaagaaaat gggtaaacat
     3301 agtgggttga tgtattacac aataggacaa agacatggtt taggtatagg tggagatggc
     3361 gatccttggt ttgttgtcgg taaaaaccta aaagataatg ttttatatgt agaacaagga
     3421 ttccatcacg atgcattata cagtgactac ttaattgctt cagactattc atttgtaaat
     3481 ccagaagata atgacttaga tcaaggtttt gaatgtacag ctaaatttag atatcgccaa
     3541 aaagatacga aagtttttgt gaaacgtgaa aacgaccatg cactacgtgt tacttttgct
     3601 gagccagtaa gagcaatcac acctggacaa gcagttgttt tttatcaagg tgatgtgtgt
     3661 cttggtggag caacaattga cgatgtgttc aaaaatgaag gtcaattaaa ttatgttgtt
     3721 taagcaatga caacaataaa ttacttattt gaagtttcag cgttggaaat gacgaaagac
     3781 agtttttgat gagaataatt catgatgata gagtctggga catcacaatg tcctaggctc
     3841 tacaatgtta tattggcggg cccccaacac agaagctgac ggaaagtcag atttcaataa
     3901 tgtgcgagtt ggcggggccc aacacagaga atttcgtaaa gaattttaac aggcaatgga
     3961 agttggggac gggcccccaa cataaaagct gacggaaaat cagatttcaa taatgtgcaa
     4021 gttggcgggg ccccaacata gagaatttcg taaagaattt taataggcaa tggaagttgg
     4081 cggggcccaa cacagagaat ttcgtaaaga aattctacag gcaatgagag ttggggataa
     4141 caaattaatt ttgtaaaaat atcatttctg tctcacccct atgcatgagt ctaattatgt
     4201 attcctattt taagtacata atagtggtgg ctaatgtgga agaaccatta cataataaac
     4261 cgttaatggt tctttagcat ttccattcca ttcccgcttt ttcatgaatg aagatgatat
     4321 tagattatat tttattcgtt gttaagtgat tcgagacata caatttatca agatgttata
     4381 attgatgaga aatgaggttc gtaaatgata gatcaacaaa caatatatca atacatacaa
     4441 aatggaaaaa tagaagaagc gttacaagca ttgttcggaa atatcgaaga agatcctaca
     4501 attattgaaa attatattaa tgccggcata gtacttgctg atgcgaatga gattgaaaag
     4561 gcagagcgtt ttttccaaaa agctttaaca atagatccga agaatggcgt cgtattttat
     4621 aatctagcaa atgtatatta taatcagcaa cgttatcaag aatctattaa attatatcaa
     4681 caagcattac aaacagagat tgaacaagtt gattgtaatt atatgatcgg tatggcgttt
     4741 aatcagttag aatcatttaa gctggcattg ccgtatttaa tgactgctgc agaactagat
     4801 aaagacaaag atgcagaagt tcaatttcaa tatggtcttg tattatgtca attagagatg
     4861 tttaatgaag ccataactca acttaaacat gtattaacga ttgataaaaa tcatgttgat
     4921 gcaagataca atttgggttt agcgttattt atgaaaaatg agaatattga tgaagccata
     4981 actcatttta aagaagctgt gactatcgac cctaaacact tattaagtca gcatgcgctg
     5041 aaaacattca ctaaaatgaa agaggaggag taacatgtca gaccctacac tatttgatta
     5101 ttcaatgatc aaaggtactg ttgaagcgat attgtttcaa aacagtgata atttttatac
     5161 agtgcttaaa gtagatacta ttgaaacaaa tgaagacttt gatacaatgc caactgttgt
     5221 agggtttctt ccgaatattg ttgaaggcga tgtctacaca tttaaagggc aagttgttga
     5281 tcatccacgt tatggtaaac aattaaaagc agagactttt gaaaaagaaa tgccacaaac
     5341 gaaagaagca attataagtt atttatcaag tgatttgttt aaaggtgtag gtaaaaagac
     5401 agctcaaaat attgttaata cattaggtga caatgcgata aacgatatat tagatgacca
     5461 ttcagttcta gaaaaagttt cgggattatc taaaaagaaa cagaaacaaa ttgcagaaca
     5521 aatttcagca aatcaagaat ctgaaaaaat aatgattcgt ttacatgatt tagggtttgg
     5581 tcctaaactt tcaatggcaa tttatcagtt ttatctcggt gatacactga ctattttaga
     5641 tcgaaatcca tatcaattga tttatgatat taaaggtatt ggttttaata aagcagatca
     5701 acttgctaga aatattggta tcgcttataa cgataatgaa cgattaaaag ctgcgttgct
     5761 ctatacgtta gaagaagaat gtattaaaca gggacataca tatttgccta tcaatgttgt
     5821 tatagattta acggttgatg tcttaaatta tcaagatgaa gaggtcatcg aacctgaaaa
     5881 attagacgaa atgttgcaat acttaaatga ggaaaaacga ctaattattg acaatgaaca
     5941 agttgcaatt ccaagtttgt attattctga aatcaaaagt gttcaaaact tattcagaat
     6001 aaagacgcat acaaataaac ttacggaaat tgaacagtca gatttacaaa tgcatattgg
     6061 cgaaatagag gatgcgaatc aagtgaacta tgcagcttca caaaaagaag ctttgcaaac
     6121 ggctatcaat tctaaggtga tgctattaac cggtggaccg ggaacaggaa agacgacagt
     6181 aattaaaggc attgtagaat tatatgctga gattcatggc ttatcattag attatgatga
     6241 ttatgtcaat gatgattatc cagttgtatt agcagcgcca acaggaagag cgtccaagag
     6301 actacaagag tcaactggat tagaagctat gacgattcat cgtttaatag gttggaatca
     6361 agacacaaag ccagaggata tattagaaaa tgaaattaac gcacgactca taatcattga
     6421 tgagatgtct atggtagata cttggttgtt ccaccaattt ttaagtgcag ttcctttaga
     6481 tgcacaattg atatttgtag gtgatgagga ccaattgcct tctgtaggtc ctggtcaagt
     6541 atttaaagat ttaattgaat cgaaagctat accacgagtt aatctgactg aagtatatcg
     6601 tcaacaagat ggttcaagca ttatagaatt agcacatcga atgaagttgg gtcagaaaat
     6661 tgatattaca cagcgttttc atgatagaag ttttattaac tgtcaggcaa accaaatacc
     6721 aacagttgtt gagaaggtag ttactagtgc tgttaataaa gggtatacta tggctgatat
     6781 tcaagtgctt gcaccaatgt ataaaggaaa tgccggtatt aaacgtttaa atcaagtttt
     6841 acaagatatt ttaaacccta aaaagaaaga cacacgagaa attgagtttg gtgatgtagt
     6901 ctttagaaaa ggtgacaaag tattacaact tgttaatagg ccaaatgata acatattcaa
     6961 tggtgacata ggagttattg ttggtatttt ttgggcgaaa gaaaatgccc ttaataaaga
     7021 tgtgcttgtt gtggattttg aaggtaatga aattacattt acgaaacaag atatgatgga
     7081 attaacacat gcatattgta catcaatcca taaatcacaa ggttcagaat ttcctattgt
     7141 tatcatgcct attgttaaac agtattttag aatgttacaa agaccgatat tatatactgg
     7201 tttaactaga gcgaaaacca gtttagtttt acttggtgat tcagaagcat ttgatattgg
     7261 attgaaaaca aatggtcaag cgcgtttaac acaattatgt acattgttga aaaattattt
     7321 taattcagaa gatgaagaca tgctggaaaa tacagcaact aatgatacag gagcttcgca
     7381 aacgacaata gatgatcaag tagaagcgcc aatgtcatcg aatgctagtg aagaagttac
     7441 gtctgattca acaaaaacag atacaaatgt attaacggaa gctacaatgt ttaaaattga
     7501 cccaatgatt aatatgggag aaatcacgcc gtatgacttt atagaacgtt gacaacaagc
     7561 gtaaatgtaa ctaaaataga attaaattcg tataaagatg agtagttaat gatttgttta
     7621 tcagagatgt actggttggt gaaaagtaca acaaacatta gtgaacgcta ctttatgcac
     7681 aactgtatag tcgatttaaa gtgataaatg tattgaattg catttataac tagggtggta
     7741 ccgcgataac gttcgtccct tttgaggatg ggcgtttttt attttttaat aataaaaagt
     7801 gatattgatt tatactagtt tttcaaatcg actatctgtg tcatcaattt ttatggaatg
     7861 gtaatttaaa aacagtaaat aggatgtgta aatgatgaaa aagttaaaag cgagtgaaat
     7921 tagacaaaaa tatctagatt tctttgttga aaaaggacat atggttgaac cttctgcacc
     7981 attagtgcca attgatgatg atacattatt atggattaat tcaggtgtag caacattaaa
     8041 gaaatatttt gatggacgtg aaacacctaa aaagccaaga attgtaaact ctcaaaaagc
     8101 tattcgtaca aatgatattg aaaatgttgg tttcacagcg cgtcaccata cattctttga
     8161 aatgttaggt aacttctcaa ttggtgatta ctttaaacaa gaagcgattg aatttgcttg
     8221 ggaattttta acgagtgata aatggatggg tatggagcca gataaattgt acgttacgat
     8281 tcatccggaa gatatggaag catacaacat ttggcataaa gatattgggc ttgaagaaag
     8341 tcgtattatt cgcattgaag gtaacttctg ggatattggt gaaggacctt caggaccgaa
     8401 cactgagatt ttctatgatc gcggagaagc atatggacaa gacgatccgg cagaagaaat
     8461 gtatccaggt ggagaaaatg aacgctatct tgaagtatgg aacttagtat ttagtgaatt
     8521 caatcataat aaagatcata gttacacacc attacctaat aaaaacattg ataccggcat
     8581 ggggcttgag cgtatggcct cagtttctca aaatgtacgt actaactatg aaacagattt
     8641 atttatgcct ataatgaatg aaatcgaaaa agtatcaggt aaacaatatt tagtaaacaa
     8701 cgaacaagat gtggcattta aagtaattgc tgaccacatt cgtacgattg catttgcaat
     8761 ttctgatggt gcattacctg ccaatgaagg tagaggatat gtattacgtc gattgttacg
     8821 tcgtgccgtt cgttttagtc aaacgttagg aatcaatgag ccatttatgt acaaacttgt
     8881 tgatattgtt gcagacatta tggaaccata ttatccaaat gttaaggaaa aagcagattt
     8941 cattaagcgt gttataaagt ctgaagaaga acgattccat gaaacattag aagatggttt
     9001 agcgatttta aatgaattaa ttaaaaaagc taaagcgaca acaaatgaaa ttaatgggaa
     9061 agatgcattt aaattgtatg atacgtacgg gttcccaatt gaattaactg aagaaatagc
     9121 agtgcaagca ggattgaaag ttgatatgac aacattcgag tcagaaatgc aacaacaacg
     9181 tgatcgtgca cgtcaagcac gtcaaaattc tcaatcaatg caagttcaaa gtgaagtatt
     9241 gaaaaatatt acatctgcaa gtacttttgt tggttatgat actgcgacag ctcaaacgac
     9301 actaacacac ttgatatata atggtgaaga agtttcacaa gttgaagcgg gtgaaacagt
     9361 atacttcatg ttaacggaaa caccatttta tgcagtcagt ggtggacaag ttgcggatac
     9421 aggtattgta tataatgaca attttgaaat tgctgttagt gaagtaacca aagcgccaaa
     9481 tggtcaaaac ttgcataaag gagtagtaca atttggccaa gtaaatgttg gcgctacagt
     9541 gtctgctgaa gtgaaccaaa atgatcgacg tgacattcaa aagaaccata gtgcaacaca
     9601 tttattacat gcagcgttga aatcagtact gggtgatcat gttaaccaag ctggttcatt
     9661 agtagaagca gatcgtttac gttttgattt ctctcatttt ggtccaatga ctaatgatga
     9721 aattgatcaa gttgaacgct tagtaaatga agaaatttgg aaaggtattg acgttaacat
     9781 tcaagaaatg gatattgttt cagctaaaga aatgggcgca atggcattat tcggtgaaaa
     9841 atatggtgat gttgtgcgtg tagtaaatat ggcaccattt tcaattgaat tatgtggtgg
     9901 tattcatgtc cgcaatactt ctgaaattgg cttattcaaa atagtaagtg agtcaggtac
     9961 aggagctggt gtgcgtcgta ttgaagcatt aacaggtaaa gcagctttct tatatttaga
    10021 agatattcaa gagaaattta atacgatgaa atcacagctg aaagtgaaat ctgataatca
    10081 agtagtcgat aagttaacac aattgcaaga tgaagaaaaa gcattattaa aacaattaga
    10141 gcaacgtgac aaagaaatca catcacttaa aatgggtaat attgaagatc aagttgaaga
    10201 aatcaatggc tataaagtat tggttactga agtggatgta ccaaatgcga aagcaattcg
    10261 ctcgacaatg gacgatttta aatctaaact acaagataca attatcattc ttgcaagtaa
    10321 tgttgatgat aaagtatcga tggttgcaac tgtccctaaa tctttaacaa ataatgttaa
    10381 agccggtgat cttatcaaac aaatggcacc aatcgttggt ggtaaaggtg gcggtcgtcc
    10441 agatatggct caaggtggcg gtacacaacc tgaaaatatc tcaaaatcat taagctttat
    10501 taaagattac attaaaaatc tataatttaa agccataata ttgtatgatg atagtgagtt
    10561 atatttgtaa ttaaaggagt gtcgcaaatg gaaaactttg ataaaacaat gaaattcgac
    10621 tatgaagaac ttccaacaca agatgtaaga gatgttttaa ataatgttta tcgaacatta
    10681 gatgaacgag gatataatgc cgtaaaccaa attgtaggtt atttattatc aggtgaccct
    10741 gcgtatattc cacgccaaaa tgaagcaaga aatcaaattc gtcatattga tagagatgtt
    10801 attatggaag agcttgtttc ttactattta aaagagcaaa ataaataatt tatgttacaa
    10861 cataaaattt taggactcga tgtcggtagt agaacggtag gaattgcaat tagtgatata
    10921 atgggttgga cggcacaagg attagacaca ctccgaatca atgaagaaaa taatgaatta
    10981 ggtattgacc aattagtaga cattattaaa aaacataatg tgggtacagt cgtaatagga
    11041 ctacctaaaa acatgaataa ttcaatagga tttcgaggcg aagcttcgtt aacatataaa
    11101 gaaaaattat tagaagctta tccttctatt gagattgtga tgtgggatga aagattaagc
    11161 acaatggctg ctgaacgatc attattagaa gcagatgttt caagacaaaa aagaaaacaa
    11221 gtgattgata aaatggcagc ggtatttatt ttacaaggct atttagattc actacattaa
    11281 aaggagattt tactatgact gaacataatc atgattcaca actagaaatt aataacgaag
    11341 aagaattatt aactttattc gatgaagagg gaaatgaagt tttataccga aaagttttag
    11401 aattttatca tcctgaattc aaaaaagagt atgttatctt agctgaagaa ggtgctcaat
    11461 cagatgaaga cgatatgatt gagcttgtac caatgatcaa tgaaccagat gagtcaggtg
    11521 acggtggtaa gttagtacca atcgaaactg atgaagaatg ggacatgatt gaagaagttg
    11581 taaatactga aatggaagaa taattaaaaa gattttactc ttgctaacct taagatgggg
    11641 aggcaagagt atttttttaa gtcaaacaat gtataaataa ttaacgctta taattttatt
    11701 gaaaatatgg taaaatatct aaagttaaat tttatatgaa tgattcgctt ctataaataa
    11761 aatagctttg cgtctaacac gaaatcagtc atttaattgg ttggcaaaat tataatcaat
    11821 tgtgaaggct gatttatttt attgtcataa tcatattgac ttattttaca taaaggaagt
    11881 ttaaaacatg gatgacctaa ataaaaaata tttaatagat ttacatcaac atcaaaatag
    11941 ttcaatcgaa gttttgcgtg aatttgccga agtaaatgaa gtgccaatcg tggatcgttt
    12001 aacattagat ttaattaagc aattaattcg tatgaataat gttaaaaata ttttagaaat
    12061 tggtacagca atcggctata gttctatgca attcgcttct atatctgatg acattcatgt
    12121 cacaacgata gagcgtaatg aaacgatgat tcaatatgct aaacaaaatt tagttactca
    12181 tcattttgaa aaccaggttc gaattattga aggtaatgct ttagagcaat ttgaaaatgt
    12241 aaatgacaaa gtttatgata tgatatttat tgatgcagca aaagcgcaat caaagaaatt
    12301 ttttgaaata tatacaccac ttttaaagca ccaaggtctc gtaattacag ataatgtttt
    12361 atatcacggt tttgtatcgg atattgggat tgttagatcg agaaatgtaa gacaaatggt
    12421 taaaaaggtt caagattata atgagtggtt aataaagcaa ccaggatata caacgaattt
    12481 tttaaatata gacgatggat tagcgatttc aattaaagga gaatgactat gacagaatta
    12541 cttgtcacac ccaagtcatt aagccatatg gaaacactta ttgatttagg tgcagatgct
    12601 tttgtaattg gcgagcaaaa gtttggttta agacttccag gggaattcaa tagacaacaa
    12661 atgacagagg ctgtcgcatt agctcacaaa aacgataaaa aagtatatgc ggcggtgaat
    12721 ggattatttc ataattatca tttagatgct gtagaagatt atattaattt tttacatgaa
    12781 atacgagttg acagaattat tttcggggat ccagcagtag taatgtatgt gaaggcgcaa
    12841 gaaaatccaa taccacttca ttggaatgca gagacgttag taactaatca ttttcaatgt
    12901 aattactggg gtaaaagagg cgcatcaaga gctgtcttag cacgtgagct gaatttggat
    12961 gaaattatta atataaaaga aaattcaaat gtggaaattg aggtgcaagt tcaaggaatg
    13021 acttgtatgt tccaatcaaa gcgtatgcta ctaggcaatt attatacgtt ccaagatcgt
    13081 caaatgaaaa ttgaacgtcg caatgatgaa caatctttat tgctttatga tgaagaaaga
    13141 caaaataatt acccagttta cgaagattac aatggtacgc acattatgtc accaaacgac
    13201 atttgtttaa ttgaagaatt agcaccattt tttgaggcgg gtatagattc atttaaaatc
    13261 gatggtattc tacaaacgga agaatatatt aatgtggtaa cggaacagta tcgtcaagcg
    13321 atagatttgt acaatgaaga tcctgaaatc tatgaagatg agaaatttat gttgatggac
    13381 ccaattgaag aaattcaacc tgatcatcga ccatttgacg aaggtttctt atataaacaa
    13441 acagtatatt aaggaggtta atcatgaaga caatagaaga gattaaatca actcctaaaa
    13501 cagttatgaa gaaaccagaa ttattagcac ctgctggaaa cttagaaaag ttaaaaatag
    13561 cagtacatta tggcgctgat gccgtatttt taggtggtca agaatatgga ttacgttcaa
    13621 atgctgataa tttcacgatg gaagaaatag ctgaaggtgt tgaatttgcg aaccgttacg
    13681 gtgccaaaat ttatgttacg acaaatatta ttgcacatga tgagaatatt gaaggtctag
    13741 aatcatattt gcgtaatttg gaaaagactg gtgcgacagg tatcattgtt gcagatcctt
    13801 taattatcga aacatgtaaa gaagttgcgc caaaacttga aattcattta tctactcaac
    13861 aatcactttc taattacaaa gctgtagaat attggaaaga agaaggattg gatcgtgttg
    13921 tattagcacg tgagaccggc gcgatggaaa tgcgtgaaat gaaggaaaaa gtagatattg
    13981 aaatcgaagc atttattcat ggtgctatgt gtatcgccta ttcaggtaga tgtacattaa
    14041 gtaatcatat gactgcaagg gattccaaca gaggcggttg ctgtcaaagt tgtcgttggg
    14101 attatgaatt attagaagtt gatgataatg gtgaacttga tgtattttat aatcaaggtg
    14161 aagttacacc atttgcgatg agtcctaaag atttaaaatt aatcgaatct attcctcaaa
    14221 tgatggatat tggtgtggac tcattaaaaa ttgaaggacg tatgaagtca attcactata
    14281 ttgcaacagt tgtctcagta tatcgtaaag tcattgatgc gtatgcggca gatcctgaca
    14341 actttaagat taatccggaa tggttaattg agttagataa atgtgcaaat agagacactg
    14401 cacctgcatt ctttgaggga acacctggtt atgaagaaca gatgtttggt caacaacaat
    14461 ctaaaaaatc accatttgat ttttgtggtt tggtattaga ttataatgaa gatacaaaaa
    14521 ttgcgactat tcaacaacga aataacttta aaccaggtca agaaatagaa ttctttggtc
    14581 cagaaattga aacatttaca caagtagtag aagcaattta tgatgaagaa ggtaacagct
    14641 tagacgcggc gcgccatcca ttacaaatcg ttcaaattaa agtggatcgc ccgatatatc
    14701 caaacaacat gatgagaaag gaaattggct aatgaaagct actacaatca ttggcatagc
    14761 tggtggatct ggctcaggaa aaacaactgt aactaacgaa attatgaaaa acttagaagg
    14821 tcatagtgtc gctttacttg ctcaagatta ctattataaa gatcaaaagc acttgacttt
    14881 cgacgagcgc ctagaaacca attatgacca tccatttgca ttcgataatg atttattaat
    14941 tgaaaatctt aaagacttga aaaatggtaa agcagtagaa gtaccgacat atgattatgc
    15001 tagtcataca agaagtgaca ttaccattga ttttaaacct aaagatgtta ttatcgtaga
    15061 aggtattttc gctttagaaa ataaggtatt acgtgatatg atggatgtta aaatatatgt
    15121 tgatacagat gcagacttga gaatattacg ccgtttaaca cgagatacta aagagcgtgg
    15181 gcgttcaatg gactctgtta tcaatcaata tttaagtgtt gttagaccta tgcatgacca
    15241 atttattgaa ccgactaaga aatatgctga tataattatt cctgaaggtg ggagcaataa
    15301 agttgcaata gatattatga caacaaaaat tcagtcttta gttagcaagc aatagtataa
    15361 ttcagtaaag gaagatgaca atatggaaaa tcaaaagcaa tatccaatga ctcaagaagg
    15421 ttttgaaaaa ttagagcgtg aacttgaaga attaaaaaca gttaagcgtc ctgaagttgt
    15481 agagaaaatt aaagttgcac gttcatttgg tgacttatca gagaactctg agtatgatgc
    15541 agcaaaagat gaacaaggat tcatcgaaca agatattcaa agaattgagc atatgttaag
    15601 aaatgcatta atcattgaag atactggaga taacaacgtt gttaaaattg gtaaaacagt
    15661 aacgtttgta gaattaccag gtgatgaaga ggaaagttat caaatcgttg gttcagctga
    15721 atcagatgca tttaatggta agatttcaaa tgaatcacca atggctaaag cgttaattgg
    15781 taaaggttta gatgatgaag tgcgtgttcc actacctaat ggtggcgaaa tgaacgtaaa
    15841 aattgttaat atccaataaa tgatcaaata gattgtttaa ttgcgcgatt gaagtgtgtt
    15901 aatgatactt cggtcgtgct tttttatatt caaaataaaa aaatcggaca gatgaaaagt
    15961 gaaaattttc catcagaccg attgatagca gaatctaaat tagtgtaaga gtttcaatat
    16021 taactactcc attttaaaaa agcaaaatat ttccgaagaa acaaattgat tataatttgt
    16081 tattgtatgc gatttgaatt acttattaaa caacttgtgc ttctcaatga tacaatgaaa
    16141 aggtattcaa tactggttta ttgcaataca aagggggatt tagagtggat gtaagattta
    16201 ttaacgagca gacgattatg atttattttg aaaataaaat atccgaagag acatatcgaa
    16261 atgtcacagc aatggtacgg tggatacgag aaaaagaaat attagaaatt caagatattg
    16321 tcccttcgta tcgagcagtt ctaatatatt ttgatgaaca agctataaca ccttctaaat
    16381 taattgaaaa tttagaactt aacaaattta atgaaaaaaa tgttcatgct gtgaatcaaa
    16441 ccaatagtat tattaaaata cctgttcaat atggtggtac atatggacct gatattgaag
    16501 aagtagcaaa gcataatcga ataactgttg agcaagttat tgaaaaacat acaagtaaac
    16561 cttatttaat atatatgcta ggatttatgc ctggatttcc atacttaggc ggacttgatg
    16621 aacaactaca tacacctaga cgtaatcagc caaggttaaa aattcatgca ggttctgtag
    16681 gtattgcgaa taatcaaaca ggactatatc catcagattc tcctggcgga tggcaaatta
    16741 ttggacgcac accgttaaaa gtatttagca gcgagcgtga accaatgtca atgtatgaag
    16801 caggagaatg gatacaattt tatgcaatag atgaacagga atttatacaa atcgaaagag
    16861 atatatcaga tggcaacttt aatgttgatg actgggtggt gatcgaaaat gtcaattaag
    16921 attttacaac caggtctctt ttcaacggta caagatctag gaagaaaagg ttatgaacat
    16981 attgggtttt caggcgcagg tgccatggat caatttagtt ttaaggttgc gcagtcatta
    17041 attaacaatg atggtccagc gattgaatat actttgattg gtcctaccat tcaatttaat
    17101 acgcaaaata catttgttat aaccggtggt agtgttaatg cctcgctaaa taataaaact
    17161 atatcaatga attctgtcat attagctgag aaaggtgaca ttttaaaaat aggtgctata
    17221 accaaaggtg cacgcggtta tcttactttt ggtcatacta tcaacgtacc tccaattgct
    17281 gaaagttatg caacacatac ccgaagtagt ataggaggat tcaaaggtag aaagttatta
    17341 gctaatgatg taataacagt gaaaataaat aatgacttta aagaaaacat tggaaagact
    17401 attcatttac aggacgattt attgccagaa aataatatta tacacattct tcaaggacct
    17461 caattcgagg cattttctga agaggctaga gcgaaaattg taaatcatcc atatttaatt
    17521 accgaacaat cagaccgtat ggggtatcgt ttggaaggtg acagcgttgc acctattaat
    17581 caagcagata tcatttctga accggttgct cttggcagtg ttcaagtacc aaatgatggt
    17641 caacctatta ttcttcttaa tgataaacaa acgataggtg gttataccaa aattgcaact
    17701 gtatgcaaat ttgatctgcc aaagttagca cagatgaaac cacaagatac aattcaattt
    17761 aaatggatat cattccaaga agctgtagat aagaatcggg aacaaatgtc tctgtttaat
    17821 gaaattctta agagtcatca aaagactcca atttttgata catctagctt gcgacatact
    17881 tcgaaaaaat tagcaacaat tttaaaaggg gatttgtaaa atgaatattg aaaaaatcga
    17941 acaaataatc aaattagtga aggaaaatga tgttaagaaa tttaaatata aaaattttga
    18001 agatgaaata gaaattgact tcactgattc gaatcatttg gctgcacaca gtaatcaatc
    18061 aaatcaaagt atgaacaata atgatttgac agcttcaaaa gtgaatgata actccgatgt
    18121 ttcgacaaat gattatcatg acattaaatc accaatggta ggtacattct ttttacaaga
    18181 cagtaaagaa ttaactgaac caattgtgaa agtcggtgac aaagttaaca agggagatat
    18241 tataggatat gttgaagcga tgaaagtatt aaacgaggta acaacagatg ttgctggaga
    18301 aatcactgaa atagtagctg atcatggcac aaatgttgaa tacgaccaag ttttggtacg
    18361 tattaagtaa agggggtaag taaatgcttc gttgtttaat tgcgaacaga ggtgaaattg
    18421 ctgtaaggat tataagagct tgcagagaat atgggattga aactgtagca gtttatgcaa
    18481 aaggggacga gcaaagcttg catgtacatt tagcggatca agctatatgt attggagaag
    18541 ctaatgcttt agatagttat ttaaatattg accgcatcat atctgctgca caaatcactg
    18601 gtgctaatgc aattcaccca ggatatggct ttttatcaga atcaacaaaa tttgctcaaa
    18661 ccgttgagga acaaggcata gcgtttatag ggcctactaa aaaaacgatg gaaatgatgg
    18721 gggataaaat aacagcaaga caaacagttc atcatgcagg tgttcctgtg attccaggat
    18781 caaatgatgc tgtgaatcat gtgtctgaaa ttgagaagct tgccaaagac atcggttatc
    18841 cagttgttat caaagctgcc agtggcggtg gtggtaaagg tatacgtatt gtaaagaaag
    18901 ctgaagattt agaaaaggca tttaaagaag ctaaaagtga aggaaaaaaa tactttgatg
    18961 atgatcgtgt ttatgttgag gcttttatac ctgttgcaaa acatgttgaa gttcaagtta
    19021 tgggggatgg gcaggataat tatgtacatt taggggaacg tgattgttct gtacaacgta
    19081 aaaatcaaaa attaatcgaa gaatctcctt gtgcagcatt aactgaagaa agacgacaac
    19141 aaatatgtaa cgatgctgtt aaagttgcac gtgcagcaaa ttatagaagt gcaggtacga
    19201 ttgaattttt agtaactgat aacgcacatt actttattga aatgaatgct cgaatacaag
    19261 ttgaacatac tgttacagag atgcgagcag aacgtgattt ggttgcagca caattgtatt
    19321 tattggaaca taatcattta cctttttcac aaaacgatat tcaatttaat ggtcatgtga
    19381 tagaagcgag aataaatgcc gaaaatcctg aaaagaaatt tcaaccaaca ccaggtaaag
    19441 tgacagcgtt acatttacct caaggcttta atgtgcgagt cgattcttta ctatatcacg
    19501 gttatcaagt ttcaccatat tatgattctt tagtagctaa agtaattgta aaatctcatg
    19561 atagagcttc agcaattgat aaattgagag ctaccttaga tgaaatggtg atagatggat
    19621 tttctacaac tgccgatttt ttatatgcag ttttaaatta tccactatat cgtgatggag
    19681 atgcaaaaga tgttgatatt aaatttttag aaaaacatca aattgttaaa ggggtggaat
    19741 tataatgcga gttgatttga attgtgattt aggcgaagca tttggaaatt attcctttgg
    19801 tggtgatcat caaattattc cgttaattac aagtgcgaat gttgcttgtg gttttcacgc
    19861 tggtgatgaa aatgtaatga atgaaacggt aaaacttgcc aaagcacata atgttgcagt
    19921 aggtgcacat cctggtttac ctgatttgaa aggctttggc agacgaaata tagatatctc
    19981 taacgaggag atttataatt tgatgattta tcaattaggt gcattacaag ggttttgtcg
    20041 cattcatcaa gttaaaatta atcatgttaa accacatggt gcattgtatc agatgggtgc
    20101 aaaagacaga gaaatagcaa gcgttatagc acaagctgtt tatgactttg atccatcact
    20161 agtgttagta ggattagcaa attcatatct aatttcagaa gcaaagaatg tcggattaat
    20221 tacagcttct gaagtgtttg ctgatagacg atacgaagat gatgggcagc tcgttagtag
    20281 aaaagaaagt gatgctgtga ttactgatac tgacgaagca cttaagcagg ttttgaagat
    20341 ggtgaaggaa aataaagtta tttcaaaaaa caataaggaa gtaacgttac aagcagatac
    20401 aatttgtgtg catggtgatg gagaacatgc attattattt gtttcgaaaa ttagagaaat
    20461 tttaatgaaa gaaggcattg atattcaatc cttatagggg gctaaaaaat ggggagaaat
    20521 ttaaagctta aacatcgcga aagtgatttt gaatttacta aaaaccataa gagattattg
    20581 ttaggttctg tatttctaat ggcaacctct gcaataggac cagcattttt aacacaaacg
    20641 gcagtattta catcgcaatt tttggcgagt ttcgcatttg ccatattact gtctattatc
    20701 attgacattg gtgcacaaat taatatatgg cgcatattag ttgtaactgg tttaagaggt
    20761 caagaaatat caaataaagt tgttcctggg cttggtactg ttatctcaat attgattgca
    20821 tttggtggtc tagcttttaa tattggtaat attgctggtg ccggtttagg tttaaatgca
    20881 atttttggat tagatgtaaa atggggcgca gctattactg caatctttgc aatattaatc
    20941 tttgtaagta aaagtggaca aaaaattatg gacgttgttt caatgattct tggtattgtg
    21001 atgattttag ttgtggcata tgtgatgttt gtttctaatc caccttatgg tgatgctttt
    21061 gtgcatacat ttgcgccaga acatccaatg aaattagtct tgccaatcat tacgttagtt
    21121 ggtggaactg taggtggtta tattaccttt gcaggtgcac atcgtatatt agactctggc
    21181 attaaaggta agcaatattt accatttgta aatcaatcag caattgcagg tattttaact
    21241 acaggtatta tgagaacgtt acttttccta gcggtattag gagttgttgt aacaggtgtg
    21301 acactaagtt ctgaaaatcc accagcgtca gtttttgaac acgcaattgg accaattgga
    21361 aagaatattt ttggtattgt gttatttgct gcagctatgt catcagtaat tggctcagca
    21421 tacacaagcg caacattttt aaaaacactt cataaatcac ttaaagaaag aagtaattta
    21481 attgtgattg tgtttatcgt tatttcaaca atgattttct tatttattgg aaaaccaatc
    21541 agccttttaa ttatagcagg cgcgataaat ggatggattt tacctattac gttaggtgca
    21601 attttgattg caagtaaaaa gaaatcaatt gtaggtgatt ataaacaccc aaattggatg
    21661 ttcatctttg gtatcgttgc agtacttgtt acaatattga caggtatatt ctcattcaaa
    21721 gaagtgcttc agttatttta aaagacattt cgactaatag catcactgta aatgttgtta
    21781 gtattaggcc gaaatgtgtt ttgattatcg gtgattcatt tatacagcat aattagatat
    21841 attttaagag agagtggtgc atgacaagtg tactgctctt tttgttgtgt atcagtattg
    21901 tgatgtttta attcgaatat tatatgaatc attatgaaat ttaataaaaa taattgaaat
    21961 aattatcatt ttttctgata tactgttaaa cggtttggaa tttttaagtt tacactgtat
    22021 tggttgatat aactcaacta ataattgcga acagagtatt tcaaattgaa aagtattatg
    22081 agtgtgatac ataaatcaaa attgtaggct caagggagtg ggacagaaat gatattttcg
    22141 caaaatttat ttcgtcgtcc caccccggca aggttgacta gaattgaaaa aagcttgtta
    22201 caagtgcatt ttcgttcagt caactactgc caatataact tcgtagagca tagaacattg
    22261 atttctttcc tagcctgttt atcatttatg tctcgctctc aaatgtaaat taataattgt
    22321 tttgggggag tttgaagtaa aatatttaac aggatttatt ttaatattat tgttagaagg
    22381 aatttttaca aattcagcaa gtgcaatcga atattcagac ttacatcata aaagtaaact
    22441 tgattccaag cgtctatata atgctaaggt gtcatttgct aatccaactg atcttgaaaa
    22501 taaaaaaaca aatgatagac tgttgaagca tgatttgtta tttcatgaca tgttcttaaa
    22561 tgatgcttgg aaaaaagatt taaaagttga attcgaaaat gaggcacttt caaagaaatt
    22621 tataaataag aatattgatg tatatgctgg aagctatagc tatgaatgtc atgggggaga
    22681 aaccaataaa acgcaatgta gttatggtgg tgttacttta agtgacaata ataaatacga
    22741 tgattataag aatatacctt gtaatttatg gattgacgga catcaaacag aaatagaact
    22801 aactgcagtg aaaacgaaaa agtaaattgt taccattcaa gaattagatg ttcaattaag
    22861 aaattatttg aatgagaagt ataagttgta cgaacaaggt ggcgacattg ttaaagggta
    22921 tgttaaatat cataatgatg atgaaaaaaa tgtagaatat gatttttata atttaaatgg
    22981 tgagtatggt catgaggtat taaaaatgta tgctgataat aaaactatca atagtgacaa
    23041 attgcattta gatatctatt tattcaaatc ataatttaag tgagttaatc attaatgatc
    23101 tttgacagtg aaatataaag tacctgtcat tatattgtta caaataaaaa cagcagtaag
    23161 gaggctatta ctcagaaaag tcttactgct gttttctatt agaataatct ttaatgcaca
    23221 caggaattcg atacgaaaca aatatgatta actgtataat tacaaaataa aactactata
    23281 gtaaaaagaa gataaagacg atgataataa atgcatcgaa tataattttc ttaatgtttt
    23341 tatcaacaac atcaggtaag gacataagta taaatcctag aattagagtg tagcgacata
    23401 tgttcataat tgttgctgta aactcgatat taaatgaaaa ctttaaaaat atacttataa
    23461 ataaaatgac aaacgacgca attgcaataa aactgccgta tgtagctatc tttgttacga
    23521 gactattttg attattttca gccattgtaa aatcctctta tttcatcatt attattttaa
    23581 acatatcatt ttcctgttat ttaataaagt ttcttgacac tattggttga tgtaattgat
    23641 atgattagtt tttgaaaaat acacaaatta gaagcatcta tttcactgtt atgagttcat
    23701 aaataattat ttatgatacc aaggtattgt gaaagctttt tataaaaata aagcaaaagt
    23761 tttatacagt aatgaaaatc atgatacttg tcgttgtagt gattaaaatg tgataaaata
    23821 acaaatgcta ggatttttta aaagaggtga atcaaaggat gattggtata attggtgcca
    23881 tggaagaaga agtaacaata ttaaaaaata aattaacaca attaagcgaa atttcagttg
    23941 cacatgttaa attttatact ggcattttaa aagatagaga agtagtgatt acccaaagcg
    24001 gcattggaaa agttaatgct gcaatttcta cgacattatt aattaataag tttaaaccgg
    24061 acatcattat taatacaggt tctgctggag ctttagatga aagtttaaat gtaggtgacg
    24121 ttcttataag tgatgatgta aaatatcatg atgcggatgc aacagcattt ggttatgaat
    24181 atggacaagt accacagatg ccggtagcat ttcaatcaag taaaccttta atagaaaaag
    24241 tatctcaagt tgtacaacaa caacaattaa cagctaaagt aggcttaatt gtaagtggtg
    24301 atagctttat cggtagtgtt gaacaacgcc aaaaaattaa aaaagcattt ccaaatgcga
    24361 tggcggttga aatggaagca actgcaattg cacaaacatg ttatcaattt aatgtaccat
    24421 ttgttgtagt tcgtgcagtt tcagacttag caaatggaga agcggaaatg agtttcgaag
    24481 catttttaga aaaagcagcg gtatcatcaa gtcaaactgt tgaagcatta gtgtctcaat
    24541 tataaaaagg taaaggtgag ttaaatgggt ttagttcgca agttttttat gccgaattca
    24601 tatgttcaat caatatttca aattgattta gacaagttag tggacaaagg cgttaaaggt
    24661 attattacag atttagataa tacgctagta ggttgggatg ttaaagaacc tacagaacgt
    24721 gttaaagcat ggtttaagga agctaatgaa aaaggaatca ctattacaat tgtgtctaat
    24781 aataatgagt ctcgtgttgc tagttttagt cagcatttag acatcgattt tatttttaaa
    24841 gcgagaaagc caatgggaaa agcgtttgat aaagcaataa ctaagatgaa tataagacct
    24901 gatcaaactg ttgttatagg tgaccaaatg cttactgatg tatttggtgg taaccgtcga
    24961 ggtttatata caattatggt cgttccagtt aaacgaacgg atggctttat tactaagttt
    25021 aatagattaa ttgaaagacg attattacgt catttcagta aaaaaggtta tatcacatgg
    25081 gaggaaaatt gattgtctga cattttaaaa tgtatcggtt gtggtgcgcc acttcaatct
    25141 gaagataaaa ataaacctgg gtttgtacca gagcataata tgtttggtga tgacgtgatt
    25201 tgcagacgtt gtttccgctt gaaaaattat aacgaagttc aagatgtagg attagaaagt
    25261 gaagactttt taaaattatt atcaggactt gcggataaaa agggtattgt cgtcaatgtt
    25321 gtggatgtat ttgactttga aggatcattt attaatgcag ttaaacgtat tgtcggaaac
    25381 aaaaaaatca ttttagcagc aaataaattg gatttattgc cgaagcaaat taataaacgt
    25441 cgagttaaag aatggctaaa acgaacagca agaaaatata gtttggaagc tgacgatgtc
    25501 gtattaattt cagctgaaaa aggctggggc atagacgact tattatcatc aattgcgaat
    25561 attcgagaaa atgaagatgt gtatattgta gggacaacga atgttgggaa atctacattg
    25621 attaataaac tgattgaagc tagtgttggt gaaaaagatg tagtaacaac ttcaagattc
    25681 cctggaacaa ctttagatat gatagatatt ccattagatg aaacatcatt tatgtatgat
    25741 acaccaggta ttattcaaga tcatcaaatg acgcatttag ttagtgaaaa agaattgaaa
    25801 attattatgc ctaagaaaga aataaaacaa cgcgtatatc aattaaacga ggcgcaaaca
    25861 ttattcttcg gcggtctagc gcgcatagat tatgtatcag gtggtaaacg tccgttaatt
    25921 tgtttctttt ctaatgactt gaatatacat cgtactaaaa cggagaaggc caatgattta
    25981 tggcgtaatc aacttggcga tttattaacg ccacctggaa atctacaaaa ttttgatctt
    26041 aatgaggtaa aggctgttag acttgaaaca ggcaaagaga aacgcgatgt tatgatctct
    26101 ggtctaggct ttataactat aggaccagga gctaaagtaa tcgttcgtgt tcctaaaaat
    26161 gttgatgttg tattaagaaa ttctatttta taaggtgatt taaaaaatga aatttgcagt
    26221 tatcggaaat cctatttcac attccttgtc gcccgttatg tatagagcaa attttaattc
    26281 tttaggatta gatgatactt atgaagcttt aaatattcca attgaagatt ttcatttaat
    26341 taaagaaatt atttcaaaaa aagaattaga tggctttaat atcacaattc ctcataaaga
    26401 gcgtatcata ccgtatttag atcatgttga tgaacaagcg attaatgcag gtgcagttaa
    26461 cactgttttg ataaaagatg gcaagtggat agggtataat acagatggta ttggttatgt
    26521 taaaggattg cacagcgttt atccagattt agaaaatgca tacattttaa ttttgggagc
    26581 aggtggtgca agtaaaggta ttgcttatga attagcaaaa tttgtaaagc ccaaattaac
    26641 tgttgcgaat agaacgatgg ctcgttttga atcttggaat ttaaatataa accaaatttc
    26701 attggcagat gctgaaaagt atttagctga attcgatatt gttattaata caacaccagc
    26761 gggtatggct ggaaataacg aaagtattat taatttaaag catctttctc ccaatacttt
    26821 aatgagtgat attgtttata taccatataa aacacctatt ttagaggaag cagagcgcaa
    26881 gggaaaccat atttataatg gcttagatat gtttgtttac caaggtgcgg aaagctttaa
    26941 aatttggact aataaagatg ctgatattaa ttctatgaaa acagcagttt tacaacaatt
    27001 aaaaggagaa taacatatgc ttactggcaa acaaaaaaga tacttaagaa gtttagcaca
    27061 caatattgat ccgatttttc aaattggaaa aggcggtatc aacgaaaaca tgattaaaca
    27121 aatagatgat acgttagaaa acagagaatt gattaaagta catgtactac aaaataactt
    27181 tgatgataaa aaagaattag ctgaaacatt aagcgaagct acgcatagtg aattagtgca
    27241 agtgattgga tctatgatcg tgatttatag agaatctaaa gaaaataaag aaattgaatt
    27301 gccataataa tgaaaaggat agtactttac ggcggtcagt ttaaccctat ccatactgca
    27361 catatgatag tagctagcga agtatttcat gaattacagc cagatgaatt ttatttttta
    27421 cctagtttta tgtctccatt gaaaaagcac catgatttta tagacgttca gcacagatta
    27481 acaatgatac agatggttat cgacgagctt ggttttggag atatttgcga cgatgaaatt
    27541 aaacgtggtg gtcaaagtta tacctatgac acgatcaagg cattcaagga gcaacacaaa
    27601 gacagtgagt tgtactttgt tattgggacg gatcagtata accaactaga gaaatggtat
    27661 caaattgaat acttaaaaga aatggttact tttgtagttg taaatcgaga caaaaatagt
    27721 caaaatgttg aaaatggtat gattgcaatt cagataccta gggtagatat aagttcgaca
    27781 atgattcgac aaagagttag taaaggtaaa tctatccaag ttcttgttcc taaatccgtt
    27841 gaaaactata ttaaggggga aggattatat gaacattgaa aaagcaaaac ggcttgcaaa
    27901 agagaaactg ccagagaaac gttataatca ttctttaaga gttgcagaaa cagcgattaa
    27961 attagcagaa atttatgatg gagacactag taaagtagaa ttagcaggtg tattacatga
    28021 tttctgtaaa tatgatgatt taggtaaaat gtatcaaatt gttcgacaat acgaattagg
    28081 taatgatcta ctgagttatg gtagtgaaat attgcatggc ccagtgtgtg cagcaatcat
    28141 ggaacatgaa tatggtatca atgatgagga agtattaatg gctatcaaat accatactac
    28201 gggacgtcaa caaatgacaa aaactgaaaa actgattttt attgcagatt acatcgaacc
    28261 tggtagaaca atcccaggag ttgatgatat tcgagatatg gcatacaatc aaggtagttt
    28321 agataagaca atttatgaaa tttctaaacg cacagtacta tttttaatac agaaagatat
    28381 tacggtatat aataagacga ttgactgttt aaattattat aactatagtg acgaaagaat
    28441 aaaggatgat taaatgaatt cacaagaatt attagcaatt gctgtggatg caattgacaa
    28501 taaaaaaggc gaagatacga tttctttaga aatgaaaggt atcagcgata tgacagatta
    28561 ttttgttgta acgcacggaa ataatgaacg acaagttcaa gcgattgcta gagcggtgaa
    28621 agaagtagcc aatgaacaaa atatagaagt aaaacgtatg gaaggttaca atgaagcgcg
    28681 ttggatatta attgacttag ctgatgttgt ggtacatgtt ttccataaag acgaacgaaa
    28741 ttattataat attgaaaagt tatatcaaga tgcaccatta gaatcatatg gtcaggttgc
    28801 gtattaatta tgtcgcaata tgcagaaatg agcctagtgt acgatcaatt gactcaagat
    28861 caaccatatg aaaaatggtt tgaaattgta aaaaatcact gcaaagatga atcaaatatt
    28921 ttagatattg gatgcggtac tggtagttta acagttcaat tagaagcttt aggtaatgtt
    28981 actggtatgg atttaagtgt tgatatgttg actgttgcag ctcaaaaatc agcaaatgta
    29041 aattggcttg aaggagatat gacatcgttt gatttgcaac aacaatttga tatcatcact
    29101 attttttgtg actctctaaa ttatttgcaa gatgaaactg ctgttattga aacattcatc
    29161 aatgtttatc gtcatctggc tgattcaggt gtatttattt ttgatgtaca tactgtttat
    29221 aaaatgatga ctttgtttaa taatcaaagt tatattgatg ataaagggga cattttttta
    29281 gcatgggatg ctgtgcaagg agatttacct ttaagtgttt atcatgatat gacatttttc
    29341 attcgacatg aagatgaaac atattcaaga tttgacgagt ctcattttca gagaacattt
    29401 gatgaaaaga catatttgtc ttggttaact caagttggtt ttaaacatgt tgaaacattt
    29461 actgatttta atatagatga acataatgaa gatgcagaaa gattgttttt cattgcgaaa
    29521 aaataaaagt aaaaccactc gctcagacgt gtataaagtt gaggagtggt tttattgtat
    29581 caatttttat tacgctataa agatttttta gctcaatgga agttatatat tataagtgct
    29641 gttgttttaa ttatggtatt aattggtttt atattctgga gacaagatga ttatacttca
    29701 agagattttg aaaataaaga tactgttctg aaacaaagca ctagtgaaaa tagtagtttg
    29761 tccaaattag aagatgtcca ggtcaaagat ggagataatt ccaaaaataa aggtcctgta
    29821 tatgtcgata taaaaggtgc tgttaaacat cctaatgttt ataaaatgac atctaaggat
    29881 agagtagttg atttacttga taaagcacaa ttattagatg atgcagatgt aagtcaaatt
    29941 aatttgtctg aaaaattaac agatcaaaaa atgattttca ttcctcataa aggacaaaag
    30001 aatgttgaac cacaatttgg agcaaacagt gtgcacgtaa aaaatgggaa cacaaataat
    30061 actaaagtaa atttaaatac ggcatctgta tcagaattga tgtctgttcc tggagtaggg
    30121 caagctaaag ctaatgcaat tgttgaatat cgcaaccaac aaggtgcatt tcaagaaatt
    30181 gacgatttga aaaaagtaaa aggttttgga agtaaaactt ttgataaact gaattcttat
    30241 ttcacgacat aatggattat tttaattgca aattttattt tttgtgatta atcttatatt
    30301 attagaaaat gttactaaat tttaagtatg gaaggtgaac atcttggaaa gaatcaaatg
    30361 ggaagaatat tttatggcac aaagtcattt gctagcatta cgttcaactt gtcaaagatt
    30421 atctgtaggt gcaacgattg ttaaggataa tcgtattatt gctggtggtt ataatggctc
    30481 tgtagctggc gaggtgcatt gtatagatga agggtgttta attgaagatg gacattgtat
    30541 cagaacgata catgcagaaa tgaatgcttt attacaatgt gcaaaacaag gtgtatctac
    30601 tgaaggtgca acaatctatg ttactcattt tccatgccta aattgtacaa agtcaattat
    30661 tcaagcaggt ataaagcgta tctactatgc agaagattat cataaccatg aatatgcaac
    30721 taaattactc aaacaatctg gtattgaatt taaaaaaatt ccattttcac ctgaatatgt
    30781 tgctgaatat ctgactaaag gttgatgttt tgctgtatgt cgcgttatca atgattgtag
    30841 gagtgctttg gaattctagc aaagtgctct ctacatttct ttttatttta cttttgtata
    30901 ttgcttatcg taaaaataaa atcgtatatg cccctatttc tctcttttta atcattttct
    30961 cctcatggta tttacattat tcacaacaag caatatttaa ttatatcaat tatattgaac
    31021 gtaattctca gtttaatgag cgtgctcaag taatccaaat tcaacgtcaa ggtagtgaca
    31081 catataaagg taggttgagt ttaaaaaatg aaatatatcc tttcttttta acaaataaaa
    31141 agaattttga tttaaagaaa attgaaagtc gtaattgtat tgttaaagga caattcaaag
    31201 ttaatgacaa taagtttgta actcttaaat tacaaagtat agttgtacaa agctgcctag
    31261 aatcgaaccg gtctaattta attgagaaac ataaacagtt tataatgaat cgaatttatg
    31321 attcgggtat taagtttccg gatcgtatta tggcattgat tactggtgac gtaaaagaaa
    31381 ttaatgagca atttaaggaa cgtgttaaag aaataggtat atatcatttg ctggcagtta
    31441 gtggctcgca tatagctgca attgtattct taatttacca acctttaaaa cgattaaatt
    31501 tacctttatt tgtcattaaa ggaattacaa tcattgtatt agccttattt gctcaataca
    31561 caaattatgc acctagtgct gtaagagcta taataatgac aacgcttgta ctgcttatta
    31621 ctaatcaaat taaaataaag ggtattcagc ttttagcatt tgcatttata attatgttta
    31681 ttttaaatcc actagttgtt tatgatattg gatttcaatt ttcattcatc atttcatttt
    31741 ttattatgtt actttttcct tttttacagc aattgtcgaa gttacaatca ttattcataa
    31801 ttacgtttat tgcacaatta gcttcattta tcgttgccat tccaaacttt catcaacttc
    31861 aatgggtggg atttttatct aatttgattt ttgtaccata ctattcgatt atattgtttc
    31921 ctctatctat tttattcttt attacaagtc attttattgt gggattaacg ccgctaaatt
    31981 acttggttga cctaagtttt aattttcatg actggttact agacctattc acaagaatca
    32041 agcaatcaca tttttctgtt cccaagttta atgattggat atttataata tttataattt
    32101 ccgtttatta catattttgg ttattggcta aacgtaaata tatattggtt acgttttgga
    32161 ctataattat tctgacatta ttaataacgt ttccaacaaa ttcacatcac aaaattacaa
    32221 tgttaaatgt ggggcaggga gacagtattt tatatgaagg tggtaagaac caaaatgtct
    32281 tgattgatac aggtgggaaa gtgattgatg atactaaaca acctagttat tcaatttcta
    32341 aatatcatat tttaccaacg ctaaatgaga gagggataaa tga
//
LOCUS       DATTQF010000019        31640 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.019, whole genome shotgun sequence.
ACCESSION   DATTQF010000019 DATTQF010000000
VERSION     DATTQF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.019"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            73..561
                     /locus_tag="VB241_000756"
     CDS             73..561
                     /locus_tag="VB241_000756"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP1244076.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            554..1489
                     /locus_tag="VB241_000757"
     CDS             554..1489
                     /locus_tag="VB241_000757"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP1244077.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            1486..2793
                     /gene="rsmB"
                     /locus_tag="VB241_000758"
     CDS             1486..2793
                     /gene="rsmB"
                     /locus_tag="VB241_000758"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP1244078.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNVMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVDLQLEILENVKNNVKTGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            2796..3890
                     /gene="rlmN"
                     /locus_tag="VB241_000759"
     CDS             2796..3890
                     /gene="rlmN"
                     /locus_tag="VB241_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP1244079.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            3897..4640
                     /locus_tag="VB241_000760"
     CDS             3897..4640
                     /locus_tag="VB241_000760"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HEP1244080.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            4637..6631
                     /gene="pknB"
                     /locus_tag="VB241_000761"
     CDS             4637..6631
                     /gene="pknB"
                     /locus_tag="VB241_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HEP1244081.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            6859..7734
                     /gene="rsgA"
                     /locus_tag="VB241_000762"
     CDS             6859..7734
                     /gene="rsgA"
                     /locus_tag="VB241_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP1244082.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWSAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            7735..8379
                     /locus_tag="VB241_000763"
     CDS             7735..8379
                     /locus_tag="VB241_000763"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP1244083.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            8386..9027
                     /locus_tag="VB241_000764"
     CDS             8386..9027
                     /locus_tag="VB241_000764"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP1244084.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(9408..9596)
                     /locus_tag="VB241_000765"
     CDS             complement(9408..9596)
                     /locus_tag="VB241_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP1244085.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            10039..10413
                     /locus_tag="VB241_000766"
     CDS             10039..10413
                     /locus_tag="VB241_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP1244086.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            10428..12074
                     /gene="fakA"
                     /locus_tag="VB241_000767"
     CDS             10428..12074
                     /gene="fakA"
                     /locus_tag="VB241_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HEP1244087.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKLDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLADDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            12264..14324
                     /gene="recG"
                     /locus_tag="VB241_000768"
     CDS             12264..14324
                     /gene="recG"
                     /locus_tag="VB241_000768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP1244088.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFLNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLI
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENRTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            14527..15099
                     /gene="fapR"
                     /locus_tag="VB241_000769"
     CDS             14527..15099
                     /gene="fapR"
                     /locus_tag="VB241_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HEP1244089.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            15104..16090
                     /gene="plsX"
                     /locus_tag="VB241_000770"
     CDS             15104..16090
                     /gene="plsX"
                     /locus_tag="VB241_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP1244090.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKIAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            16083..17009
                     /gene="fabD"
                     /locus_tag="VB241_000771"
     CDS             16083..17009
                     /gene="fabD"
                     /locus_tag="VB241_000771"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP1244091.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLYNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPSGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            17002..17736
                     /gene="fabG"
                     /locus_tag="VB241_000772"
     CDS             17002..17736
                     /gene="fabG"
                     /locus_tag="VB241_000772"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP1244092.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            18042..18275
                     /locus_tag="VB241_000773"
     CDS             18042..18275
                     /locus_tag="VB241_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP1244093.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            18391..19122
                     /locus_tag="VB241_000774"
     CDS             18391..19122
                     /locus_tag="VB241_000774"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP1244094.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            19269..22835
                     /gene="smc"
                     /locus_tag="VB241_000775"
     CDS             19269..22835
                     /gene="smc"
                     /locus_tag="VB241_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP1244095.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVNDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLVERKKNQSETNARYEEEQENLIELLE
                     NISSEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEVKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRAVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESLTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLNDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAMFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            22835..24085
                     /gene="ftsY"
                     /locus_tag="VB241_000776"
     CDS             22835..24085
                     /gene="ftsY"
                     /locus_tag="VB241_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP1244096.1"
                     /translation="MSFFKRLKDKFATNKENDEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLISRYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGAK"
     gene            24072..24404
                     /locus_tag="VB241_000777"
     CDS             24072..24404
                     /locus_tag="VB241_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP1244097.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKKHLSNPEQIQRY
                     IQQLEDLE"
     gene            24430..25797
                     /gene="ffh"
                     /locus_tag="VB241_000778"
     CDS             24430..25797
                     /gene="ffh"
                     /locus_tag="VB241_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP1244098.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            26232..26507
                     /gene="rpsP"
                     /locus_tag="VB241_000779"
     CDS             26232..26507
                     /gene="rpsP"
                     /locus_tag="VB241_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP1244099.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            26695..27198
                     /gene="rimM"
                     /locus_tag="VB241_000780"
     CDS             26695..27198
                     /gene="rimM"
                     /locus_tag="VB241_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP1244100.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            27198..27935
                     /gene="trmD"
                     /locus_tag="VB241_000781"
     CDS             27198..27935
                     /gene="trmD"
                     /locus_tag="VB241_000781"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP1244101.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQARVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            28038..28388
                     /gene="rplS"
                     /locus_tag="VB241_000782"
     CDS             28038..28388
                     /gene="rplS"
                     /locus_tag="VB241_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP1244102.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(28632..31238)
                     /gene="yfhO"
                     /locus_tag="VB241_000783"
     CDS             complement(28632..31238)
                     /gene="yfhO"
                     /locus_tag="VB241_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HEP1244103.1"
                     /translation="MLKKWLNSNVKQFLVITFMSVILTLILFSTHIYEYIVNGTVFSG
                     SGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMVRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFFITISILTIVALLSFKLYRFYFYRLFAIVTWVLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILQMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSSLKQHDYHSNYVNQLIKKINKNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVRKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
BASE COUNT        11749 a         4273 c         6071 g         9547 t
ORIGIN      
        1 atgatgtaag gtcgcatttt taatatattt acgttataat ggtttgatga attttattga
       61 ggagtaagag ctatggcgat taaaaagtta gtaccagcat cgcatcctat tttaacgaaa
      121 aaagcgcaag cagttataaa atttgatgat tcgttaaaaa gattattaca agatttagaa
      181 gatacaatgt atgcacaaga agctgctggc ttatgtgcac ctcaaattaa tcagtcattg
      241 caagtggcaa tcattgatat ggaaatggaa ggattattac aacttgttaa tccgaaaatt
      301 attagtcaat caaatgaaac gataacagac ttagaaggtt caattacatt gccagatgtt
      361 tacggcgaag tgacaagaag taaaatgatc gttgtcgaaa gttatgacgt caatgggaac
      421 aaagttgaac taactgcaca tgaagatgta gcaagaatga ttttgcatat tatagatcaa
      481 atgaacggta tcccttttac agaacgtgcg gaccgtattt taacagataa agaagtggag
      541 gcatatttta taaatgacta aaataatatt tatgggtaca ccagactttt caacaactgt
      601 tttagaaatg cttattgcag aacatgatgt cattgcagtc gtaacgcaac cagatcgacc
      661 tgttggacgt aaacgtgtta tgacaccacc accagttaaa aaagttgcaa tgaaatatga
      721 tttacctgta tatcagcctg aaaaattaag tggatcagaa gaattagaac aattgcttca
      781 attagatgta gatttaattg taacagccgc ttttggacaa ttattacctg aatcattgtt
      841 ggcattacca aaacttggcg caattaatgt acatgcatca ttgttgccga agtatagagg
      901 tggtgcacca attcatcagg caattatcga tggtgaacaa gaaaccggca taacaattat
      961 gtatatggtt aaaaaattag atgcgggtaa tattatttcg caacaagcaa ttaaaataga
     1021 agaaaatgat aatgtcggta cgatgcatga taaattaagt gtattaggag cagatttatt
     1081 aaaagaaact ttaccatcaa ttatagaggg tacaaatgaa agtgtaccac aagatgatac
     1141 gcaagcaaca tttgcttcca atattcgacg cgaagatgag cgaattagct ggaataaacc
     1201 aggaagacaa gtgtttaatc aaattcgagg attatcacca tggccagttg cttatacaac
     1261 tatggatgac actaacttga aaatatacga tgctgaactc gttgagacta ataagataaa
     1321 cgagcctgga acaattatag aaacgactaa aaaagccatt attgttgcta caaatgataa
     1381 tgaagctgtt gcaattaaag atatgcaatt ggctgggaaa aagagaatgt tagctgccaa
     1441 ttatttaagt ggtgcgcaaa acacactagt agggaagaaa cttatatgat agaaaacgtg
     1501 agaagtcttg cttttgacac gattcaagat atattaaatg aaggtgcgta tagtaacttg
     1561 cgtatcaatg aagtgttgtc agaaaatgaa ttaaatgtaa tggataaagc tttatttaca
     1621 gaaattgtct acggaaccgt taaaagaaaa tatacgttag atttttattt aaagcctttt
     1681 gtgaaaacaa agattaaggc atgggttagg caattattat ggatgagtat ttatcaatat
     1741 gtttatttag ataaagttcc aaatcacgcc atcattaatg aagcagttga aatagcaaaa
     1801 gaacgcggtg gctatcacaa cggaaatgtc gtaaatggca ttttacgaac aatgatgcgt
     1861 agtgacttac ctgattttaa tgaaattgca gatcctaaaa aaagaatggc aatcgagtat
     1921 agtatgccga agtggattat agatcattgg gcaacacatt atggtctcga agaaactgaa
     1981 acgattttac agtcattttt agaaacgaca tcaacaactg tgcgtgccaa cctgacgcga
     2041 gcatcattag atgatattat tgaaaagttg caagacgaag gttatgacgt tgaaaaagat
     2101 catgacttac cttattgtct ccatatagga ggacaaccaa ttattcattc tcgttcattt
     2161 aaagatggat tcgtttcaat tcaagataaa agctcaatgt ttgttgcaca cattatgaat
     2221 gtagaccgac atgatcacgt attagatgca tgtagtgcac ctggcggtaa ggcttgtcat
     2281 attgctgaag ttttaatgcc agaagggcaa gttgacgctt cagatataca tgatcacaaa
     2341 atagacttaa ttaattttaa tataaaaaaa ttacgattaa caaatattaa agcttttcaa
     2401 catgatgcga caaaacctta tgataaaaca tacgataaga tacttgttga tgcaccatgt
     2461 agcggattag gtgttatgag acacaagccg gagattaaat atactcaaag caaacaacat
     2521 attgagtcac tagttgattt acagcttgaa atattggaaa atgtaaaaaa caatgtaaaa
     2581 acaggtggcg aaatcatcta ttccacatgt acaatagagc aactagaaaa tgaaaacgtg
     2641 atttatacgt ttttgaaaaa taataaaaac ttcgaatttg aaccgtttca acatccgata
     2701 actggagagt tggtcaaaac gttacaaatc atgccgcaag actttaattc agatggattc
     2761 tttatcacta agataaaaag aaaggacaat taggaatgat aactgctgaa aagaaaaaga
     2821 agaataaatt tcttccaaat tttgacaagc aatcaatata ttcattgcga tttgacgaaa
     2881 tgcaaaactg gctcgttgaa caaggtcaac aaaaatttcg agcgaaacag atttttgaat
     2941 ggttatatca aaaaagagta gattcgattg atgaaatgac gaacttatcg aaagacttac
     3001 gacagctttt aaaagataac tttactgtta caactttaac aactgtagta aaacaagaaa
     3061 gtaaagacgg tacaattaaa ttcttatttg aattacaaga tggctataca attgaaactg
     3121 ttttaatgag acatgattat ggaaattcag tatgtgtaac gacacaagta ggttgtcgta
     3181 tcggatgtac gttttgtgct tctacacttg gcggcttaaa aagaaacctt gaagctggcg
     3241 aaattgtttc acaagtttta acagttcaaa aagcccttga tgctacagaa gagcgcgtat
     3301 ctcaaattgt cataatgggt atcggtgaac catttgaaaa ttatgatgaa atgatggact
     3361 ttttaagaat cgtcaatgat gataacagtt taaatattgg tgcacgtcac attacagtat
     3421 caacatcagg tatcattcct agaatatatg actttgcgga cgaagatatt caaattaatt
     3481 ttgctgtaag cttacacgcc gcaaaagatg aagtgcgatc acgcttgatg ccaattaacc
     3541 gtgcatataa tgttgagaag ttaatcgaag caattcaata ttatcaagaa aaaacaaatc
     3601 gtcgtgttac ttttgaatat ggtctgtttg gtggtgtgaa tgaccaacta gaacatgcaa
     3661 gagaattagc acatttaata aaaggcttaa actgccatgt taacttaatt cctgtcaacc
     3721 atgttccaga aagaaattat gtaaaaacgg ctaaaaatga tatctttaaa tttgaaaaag
     3781 aattaaagag actaggaatt aatgccacaa tacgtcgtga acaaggttca gatattgacg
     3841 cagcttgtgg tcaattaaga gcaaaggaac gacaagtaga aacgaggtaa agacaaatgc
     3901 tagaggcaca attttttact gatactggac aacatagaga taagaatgaa gatgcgggtg
     3961 gtatttttta taatcaaact aatcaacaac ttttagttct gtgtgatggt atgggcggcc
     4021 ataaagcagg agaagttgca agtaaatttg ttacagatga gttgaaatcc cgttttgaag
     4081 cggaaaatct tatagaacaa catcaagctg aaaattggtt gcgtaataat ataaaagata
     4141 taaattttca gttatatcac tatgcacaag aaaatgcaga atataaaggt atgggtacaa
     4201 catgtgtttg tgcacttgtt tttgaaaaat cagttgtgat agcaaatgtc ggtgattcta
     4261 gagcctatgt tattaatagt cgacaaattg aacaaattac tagtgatcac tcgtttgtta
     4321 atcatcttgt tttaacgggt caaattacgc cggaagaagc atttacacat ccacaacgta
     4381 atattattac gaaggtgatg ggcacagata aacgtgtgag tccagatttg tttattaagc
     4441 gattaaattt ttatgattat ttattattaa attcagatgg attaactgat tatgttaaag
     4501 acaatgaaat taagcgtttg ttagtaaaag aaggtacaat agaagatcat ggtgatcaat
     4561 taatgcaatt ggcattagat aaccattcga aagataacgt tactttcata ctcgcggcta
     4621 ttgaaggtga taaagtatga taggtaaaat aataaatgaa cgatataaaa ttgtagataa
     4681 gcttggcggc ggtggcatga gtaccgttta tcttgcagaa gatacgatac ttaacattaa
     4741 agttgcaatt aaggcgattt ttataccacc tagagaaaaa gaagaaacat taaaacgttt
     4801 tgaacgagaa gtacataact catcacagct atcacatcaa aatatagtaa gtatgatcga
     4861 tgttgatgaa gaagatgact gttactactt agtaatggaa tatattgaag gtccgacttt
     4921 gtctgagtat attgaaagtc atgggccatt aagtgttgac acggcgatta attttacgaa
     4981 tcaaatattg gatggcatta aacatgcgca tgatatgcgt attgtacata gagatattaa
     5041 gccacaaaat atattaattg acagcaataa aacgttgaaa atatttgatt ttggaattgc
     5101 taaagcttta agtgagacgt ctttaactca gactaatcat gtgttaggta ctgtgcagta
     5161 cttttcgcca gaacaagcaa aaggtgaggc aacggatgaa tgtacagata tttattctat
     5221 aggtattgtg ttatatgaaa tgcttgttgg tgaaccacca tttaatggag aaactgcagt
     5281 tagcattgcg attaaacata ttcaggattc tgtgccaaat gtgacaaccg atgtacgtaa
     5341 ggatattccg caatctttaa gtaatgtcat tttacgcgct acagaaaaag acaaagcgaa
     5401 tcgttacaaa acaattcaag aaatgaaaga tgatttgagt agtgttttac atgaaaatcg
     5461 agcgaatgaa gatgtctatg aactcgataa aatgaaaacg atagcggtac cattgaaaaa
     5521 agaagatcta gcaaagcata ttagtgaaca taagtcgaat caacctaaac gtgaaacgac
     5581 gcaagtacct attgtaaatg ggcctgctca tcatcagcaa ttccaaaagc cagaaggtac
     5641 cgtgtacgaa ccaaaaccta aaaagaaatc aacacgaaag attgtgctct tatcactaat
     5701 cttttcgttg ttaatgattg cacttgtttc ttttgtggca atggcaatgt ttggtaataa
     5761 atacgaagag acacctgatg taatcgggaa atctgtaaaa gaagcagagc aaatattcaa
     5821 taaaaacaat ctgaaattag gtaaaatttc tagaagttat agtgataaat atcctgaaaa
     5881 tgaaattatt aagacaactc ctaatacagg tgaacgtgtt gaacgtggtg acagtgttga
     5941 tgttgttata tcaaaaggcc ctgaaaaggt taaaatgcca aatgttattg gtttacctaa
     6001 ggaggaagcc ttgcagaaat taaaatcgtt aggtcttaaa gatgttacga ttgaaaaagt
     6061 atataataat caagcaccaa aaggatacat tgcaaatcaa agtgtaaccg caaatactga
     6121 aatcgctatt catgattcta atattaaact atatgaatct ttaggcatta aacaagttta
     6181 tgtagaagac tttgaacata aatcctttag caaagctaaa aaagccttag aagaaaaagg
     6241 atttaaagtt gaaagtaagg aagagtatag tgacgatatt gatgagggtg atgtgatttc
     6301 tcaatctcct aaagggaaat cagtagatga ggggtcaacg atttcatttg ttgtttctaa
     6361 aggtaaaaaa agcgactcat cagatgtcaa aacgacaact gaatcggtag atgtaccata
     6421 cactggtaaa aatgataagt cacaaaaagt taaagtttat attaaagata aagataatga
     6481 cggttcaaca gaaaaaggta gtttcgacat tactagtgat caacgtatag acattccttt
     6541 aagaattgaa aaaggaaaaa cagcaagtta tattgttaaa gtagacggta aaactgtagc
     6601 tgaaaaagaa gtcagctatg atgatgttta aatataattg aagtaaatgt accgaggttt
     6661 ctatttggaa gtctcggtat ttttatgttg agattgcggt agttttaaaa tgcttcatgt
     6721 catcatatac aggtgagcat tgtctatgaa ttctgtatca ctctcacaat atttatatca
     6781 tttgaaatga tcatttatat attaattttc cagtctttga tataataaat gcagtaaaat
     6841 taatcgagag gtgccatttt gaagacaggt cgaatagtga aatcaattag tggggtatat
     6901 caagtagacg ttaatggcga acgtttcaat acaaaaccac gaggattatt tagaaagaaa
     6961 aaattttcac cggtagttgg agatatagtg gaatttgatg tacaaaacat taacgaaggc
     7021 tatattcatc aagtgtatga gcggaaaaat gagttgaaaa gaccacctgt aagtaatata
     7081 gatacactag taattgtaat gagtgctgtc gaaccaaatt tttcaacgca attattagat
     7141 cgatttttag ttattgcaca ttcgtatcag ttaaatgcga gaattttggt gacaaaaaaa
     7201 gataaaacac caattgaaaa gcagttcgaa attaatgagt tgttgaaaat atatgaaaat
     7261 attggctatg agactgaatt tattggaaat gatgatgatc gaaaaaaaat tgtagaagct
     7321 tggtcagctg gacttatagt acttagtggt caatcaggtg tcggtaagtc cactttctta
     7381 aatcattatc gtccagaact taatcttgag acaaatgata tatcaaaatc attaaatcga
     7441 ggaaagcata ctacaagaca tgtcgaacta ttcgaacgtc aaaacggtta tattgcagac
     7501 acacctggat tcagtgcttt agattttgat catatagata aagatgaaat aaaagattat
     7561 tttcttgaat taaatcgata tggtgaaaca tgtaagttta ggaattgtaa tcatatcaaa
     7621 gaacctaatt gtaatgttaa gcatcaatta gagataggga atattgcgca atttagatac
     7681 gaccattatt tacaactatt taatgaaatt tcaaatagaa aggttagata ttaaatgaca
     7741 aaactatatc catcattatt atctgttgat tttttggatt tacaacatga attaaaacga
     7801 cttgaagaag caggtgtcga cggagttcat tttgatgtta tggatggtca atttgtgcct
     7861 aatatatcta ttggtttacc aatattagat gcagtaagaa aaggcacaac attacctata
     7921 gacgtacatt tgatgattga aaatccagaa aagtatattg catcatttgc agaacatggt
     7981 gccgatatga tttcaattca tgtcgaatca acgcctcata ttcatcgtgc tattcaaatg
     8041 attaaacatt tagataaaaa agctggtgta gtaattaatc ctggtacacc aatatcacaa
     8101 attgaaccta ttttagatat tgttgattat gtactagtga tgacagttaa cccagggttt
     8161 ggtggtcaat catttattga tcaatgcgta gaaaaaatag cgggtcttaa tgctattaaa
     8221 atggaacgtc aattaaactt tgatattgaa gttgatggag gcgtaaatac cgatacagcg
     8281 aaagtatgtg ttgaaaatgg tgctacaatg ctagtaacag gttcattttt ctttaaacaa
     8341 gaggattata aaaaagtcac acaagcattg aaaggttgag tgtatatgca tataaattta
     8401 ttatgttctg atcgacactt gccgcaagat atttgggcca aaagtaatga aggtaaatgg
     8461 ggcggcgttg atagaggtgc tttgatttta ttgaagcatc aaattatccc ttttttctca
     8521 gtgggagact ttgattcagt cagtaaagaa gaacgccaac ttctaacaga acagttacaa
     8581 atcaaaccag ttcaagctga aaaagctgat acggatttag ctttagcggt tgataaagct
     8641 gttgcacttg gatttgatag tattacaatt tatggtgcaa caggcggacg attagatcac
     8701 ttttttgggg caattcagtt attattgaaa aaagcatatt ataaacatga tgttcatata
     8761 gaagttatcg atcaacaaaa taaaattgaa ttattgccta aaggtcaaca tacagttgaa
     8821 aaagataaga gttatccgta catttcattt ataccgatga ctgatgatgt agaactttct
     8881 ctagcaggtt ttaaatataa tttagctaga caaatgctta atataggttc tactttaact
     8941 atttcaaatg aaattgagtc tttgcaagcg aaagtaactg tacatgatgg gttgattttg
     9001 caaattagaa gtacagattt aaattaagta tattcaaata tgcgaagatt tttaataaag
     9061 tccatgagtt atttaataaa gtgacgttta tagtatagaa tttatagcat aataatagat
     9121 gaatcacgct gtaattaatg aagattatgg cgtgatttat tattgaaagg aaaattgtag
     9181 gtcctagtac attgatttat gtgccaaaat cgatattgaa cccataagtc caatttgaca
     9241 gagaaccatt attaaacgtt ttaaccaaat tatattgata gttcgaattt cactatgaac
     9301 cattatgtat tgttttgtaa caaaaaagaa ctaagaggtt gagctcttag ttcttaagta
     9361 aatgcgtcct ccacataagg tcacattttt tatgtgcaat atatttatta aactctagtt
     9421 actttaccag attttaaagc acgtgcagaa acccaaactt ttttaggttt accgtcaact
     9481 aggattctaa ctttttgaag gttagcgttc catctacgtt tagtagagtt taaagcgtgt
     9541 gaacgtctgt taccagtcga agctttacga cctgttacga aacattgttt acccatatga
     9601 gtacctcctt taataaatat aaatacacat aactacttat atacttaatt aagatagcat
     9661 agtttcattt gaaaaacaat gaataatttt cacataaaag tcaaaaatac tgagttttgt
     9721 gatataattg tagactgtga agttatgtag tatgatattt tagagaaata agtgaatgaa
     9781 acatttaaac tttttattta atgatactac atctattaaa agaaaatgcc tatacataat
     9841 cgtcatgagt gtaaattcag tttactcaaa atataatagg tgatttaagc gtaagtcatt
     9901 ctgataagtt gtaccattga tattttataa aaataatcaa tgaaacttaa agcgtttatg
     9961 actacactaa gtggtaaaat tttaaaagtg attaggggaa acgaataata aactttgaaa
    10021 tttaggaggg acaaagatat gacattagag atttcaaatg attacggtaa aattgatatt
    10081 tcaaacgaag tgattgcttc ggttgtaggt ggaaaggccg ttgaatgtta tggtattgta
    10141 ggtatggcat ctagacaaca agttagagat ggtattgcgg aaatactagg acatgaaaat
    10201 tatgctaaag gcatcaaagt aactgaaaat aatggcgtag tggatataga tatgtacatt
    10261 attgtaagtt acggtgtgaa aatatctgaa gttgccaata atgtacaatc aacagtgaaa
    10321 tatactttgg aaaaatcact taatgtatca gtaaattcaa tcaatatata tgtacaaggt
    10381 gtacgtgtga ataatacagg caagaaagct taggaggaca acttgaaatg attagcaaaa
    10441 ttaatggtaa attatttgcc gatatgatta tacaaggggc acaaaattta tctaacaatg
    10501 cagatttggt agattctttg aatgtgtatc cagtgccaga tggtgataca ggaacaaata
    10561 tgaatcttac tatgacttca ggtcgcgaag aagtagagaa taatttgtcg aaaaatatcg
    10621 gcgaattagg taaaacattc tcgaaaggtt tactaatggg tgcaagaggt aactctggtg
    10681 tcatcttgtc acaattattc agaggatttt gtaaaaatat tgaaagtgaa tctgaaatta
    10741 attcaaaatt gttagctgaa agttttcaag ctggtgttga aacggcatat aaagctgtta
    10801 tgaaaccagt tgaaggtaca atacttacag ttgcaaaaga tgctgcgcaa gctgcaatag
    10861 aaaaagcaaa taatactgaa gattgtatag aattaatgga gtacattatt gtaaaagcca
    10921 atgaatcact tgaaaacaca ccaaacttat tagctgtact taaagaagtt ggtgttgttg
    10981 atagtggcgg taaaggtttg ttatgcgttt acgaaggatt cttaaaagcg cttaaaggtg
    11041 aaaaagttga agccaaagtt gcaaagctag ataaagatga atttgtacat gatgaacatg
    11101 atttccatgg tgtaattaat actgaagata ttatttatgg ctattgtact gaaatgatgg
    11161 ttcgttttgg aaagaataaa aaagcctttg atgaacaaga attcaggcaa gatatgagtc
    11221 aatttggtga ttctttatta gtcattaatg atgaagaaat tgtgaaagtt cacgtgcata
    11281 ccgaataccc aggtaaagtg tttaattatg gtcaacaata tggtgaatta attaaactta
    11341 aggttgaaaa tatgagagaa cagcatcgtg aagtgattcg aaaagaacag cacacagcta
    11401 aaccgaaaat ggaaacggtt gaaacagcaa ttattactat ttctatgggt gaaggtattt
    11461 cagagatatt taaatcaatg ggtgccacac atatcattag tggtggacaa acgatgaatc
    11521 cttctacaga agatatcgtt aaagtcattg aacaatcaaa atgtaaacgt gcaattattt
    11581 taccgaataa taaaaatatc ttaatggcaa gtgaacaagc agcgagtatt gttgatgcag
    11641 aagcagttgt tattccaacg aaatctattc ctcaaggtat aagcgcacta ttccaatatg
    11701 atgtggacgc aacacttgaa gaaaataaag cgcaaatggc tgattcagta aataacgtta
    11761 aatctggttc attaacgtac gctgttcgtg atacgaaaat tgatggcgtt gagattaaaa
    11821 aagacgcgtt tatgggcttg attgaagata agattgtaag cagccaaagt gatcaattaa
    11881 caacggttac tgagttgtta aatgagatgt tagcagatga tagtgaaata ttgactgtga
    11941 ttattggtca agatgcagag caagcagtta cagataacat gataaactgg attgaagagc
    12001 aatatccaga tgtagaagtg gaagttcatg aaggtggaca accaatttat caatatttct
    12061 tttcagtaga ataaaaattt aaaataaaaa actaccaatg ataaatcatc agttggtagt
    12121 tttttatttt gctattttag tgatattgcg ggttaaaagt atcgttctcg agttgctaac
    12181 aatgtcatgt tcaacttagt catgataaaa taaataacat actaaatgat acgtaaaatc
    12241 aaataaaaca taggtgattt attttggcta aagtaaactt aatagaaagt ccatattctc
    12301 ttttacaatt aaaaggtata ggtcctaaga aaatagaagt attgcaacaa ctaaatattc
    12361 atacagtgga agatcttgtt ctttatttgc caactagata tgaagataat acagtgattg
    12421 atttgaatca agcagaagat caatctaacg ttacgataga aggacaagta tatacagctc
    12481 cagtagttgc attttttgga agaaataaat caaaattaac tgttcattta atggtaaata
    12541 atattgctgt caaatgtatt tttttcaatc aaccgtattt aaaaaagaaa atcgaattaa
    12601 atcaaactat aactgttaaa ggtaagtgga atagggttaa acaggaaatt actggtaata
    12661 gggttttctt gaattcacaa ggtacacaaa ctcaagaaaa cgcagatgtt caattagaac
    12721 cagtctatcg tattaaggaa ggtattaaac aaaagcaaat acgagaccaa attagacaag
    12781 cgttaaatga tgtgacaatt catgaatggt taactgatga actaagagaa aaatataaat
    12841 tagagacctt ggactttact ttgaacacgt tacatcatcc taaaagtaaa gaggatttat
    12901 tacgtgctcg tagaacctat gcatttactg aactgttttt attcgaatta cgtatgcaat
    12961 ggctaaatag attagaaaag tcatctgacg aagcaattga aattgattat gacttagacc
    13021 aagttaaatc atttattgat cgtttgcctt ttgaactaac tgaagcacag aaatccagtg
    13081 ttaatgaaat ttttagagat ttaaaagcac caatacgtat gcatcgatta cttcaaggtg
    13141 atgtaggttc aggaaaaaca gtagttgctg caatttgtat gtatgcgtta aaaacggctg
    13201 gttatcaatc agcattgatg gtaccaactg aaattttagc agagcaacat gctgaaagtt
    13261 taattgcttt atttggagat tctatgaatg ttgcgttgtt aactgggtca gtaaaaggta
    13321 agaaacgaaa aatactttta gaacaacttg aaaatcgtac gattgattgt ttaattggaa
    13381 cccatgcatt gattcaagat gatgtgattt tccataatgt tggtttagta attacagatg
    13441 aacaacatcg atttggtgtg aatcaacgcc aacttttaag agaaaaaggt gcaatgacga
    13501 atgtgttatt tatgacagca accccgatac caagaacact agcaatatca gtttttggtg
    13561 agatggatgt gtcttcaatt aaacaattac caaaaggtcg taaacctatc attactactt
    13621 gggcaaagca tgagcaatac gataaagttt tgatgcaaat gacctcagag ttgaaaaaag
    13681 gtcgtcaagc atatgttatt tgcccgctta tagaaagttc tgagcatctc gaagatgttc
    13741 aaaatgttgt cgcattgtac gagtctttac aacagtatta tggtgtttcc cgtgtagggt
    13801 tattgcatgg taagttgtct gccgatgaaa aagatgaggt catgcaaaag tttagcaatc
    13861 atgagataga tgttttagtt tctactactg ttgttgaagt aggtgttaat gtaccgaatg
    13921 caacttttat gatgatttat gatgcggatc gctttggatt atcaacttta catcagttac
    13981 gcggtcgtgt gggtagaagt gaccagcaaa gttactgtgt tttaattgca tcccctaaaa
    14041 cagaaacagg aattgaaaga atgacaatta tgacacaaac aacggatgga tttgaattga
    14101 gtgaacgaga cttagaaatg cgtggccctg gagatttctt tggtgttaaa caaagtggat
    14161 tgccagattt cttagttgcc aatttagttg aagattatcg tatgttagaa gttgctcgtg
    14221 atgaagcagc tgaacttatt caatctggcg tattctttga aaatacgtat caacatttac
    14281 gtcattttgt tgaagaaaat ttattacatc gcagttttga ctaattacca tgctgatttg
    14341 tcaatttaag tgcaacactt cgttaattga gtgatatgac acttgaacta tttaaatgta
    14401 aagtggtatt ttaaaaattt ataaattttc gactaaataa tagctaaata atacagttat
    14461 ttgttgagtc ggttaaatag aaagtgttat gatatgtgag gaatgtttaa gactaggtac
    14521 taaaaaatga ggggtgagac gttgaaacta aagaaagata aacgtagaga agcaatcaga
    14581 caacaaattg atagcaatcc cttcatcaca gaccatgaac taagcgactt atttcaagtg
    14641 agtatacaaa caattcgttt agatcgcact tatttaaaca taccagaatt aaggaagcgt
    14701 attaaattag ttgctgaaaa aaattatgac caaataagtt ctattgaaga acaagaattt
    14761 attggtgatt tgattcaagt caatccaaat gttaaagcgc aatcaatttt agatattaca
    14821 tcggattctg tttttcataa aactggaatt gcgcgtggtc atgtgctgtt tgctcaggca
    14881 aattcattat gtgttgcgct aattaagcaa ccaacagttt taactcatga gagtagcatt
    14941 caatttattg aaaaagtaaa attaaatgat acggtaagag cagaagcacg agttgtaaat
    15001 caaactgcaa aacattatta cgtcgaagta aagtcatatg ttaaacatac attagttttc
    15061 aaaggaaatt ttaaaatgtt ttatgataag cgaggataaa attatggtta aattagcaat
    15121 tgatatgatg ggtggcgaca atgcgcctga tatcgtatta gaagccgtac aaaaggctgt
    15181 tgaagacttt aaagatctag aaattatact tttcggtgac gaaaaaaagt ataatctgaa
    15241 ccatgaacga atcgaattta gacattgttc tgaaaagatt gaaatggaag atgagcctgt
    15301 tagagcgatt aaacgtaaaa aagatagctc aatggtaaaa atggctgaag ctgtgaaatc
    15361 tggtgaggca gatggatgtg tgtcagcagg taatactggt gctttaatgt cagctggttt
    15421 attcattgtt ggacgtatta aaggtgtagc tagaccggct ttagtagtaa cattgccaac
    15481 gattgatgga aaaggttttg tctttttaga cgttggtgca aatgctgatg ctaagcctga
    15541 acacttatta cagtatgcgc aactagggga tatttatgct caaaaaatta gaggtattga
    15601 taatccgaaa atctcattat taaatatagg taccgagcca gctaaaggta atagtttaac
    15661 gaaaaaatca tatgagttat taaatcaaga tcattcattg aattttgttg ggaatattga
    15721 agcgaagaca ttaatggatg gcgatacaga tgttgtagtt accgatggct atactgggaa
    15781 catggtcctt aaaaatttag aaggtactgc aaaatcaatc ggtaaaatgt taaaagatac
    15841 gattatgagt agtactaaaa ataaaatagc aggtgcaata ttgaagaaag atttagcaga
    15901 attcgctaaa aagatggatt actcagaata cggtggttcc gtattattag gattggaagg
    15961 taccgtagtt aaagcacacg gtagttcaaa tgctaaagct ttttattctg caattagaca
    16021 agcaaaaata gcaggagagc aaaatattgt acaaacaatg aaagagactg taggtgaatc
    16081 aaatgagtaa aacagcaatt atttttccgg gacaaggtgc ccaaaaagtt ggtatggcac
    16141 aagatttata taacaacaac gatcaagcaa ctgaaatttt aacttcagca gcaaacacat
    16201 tagactttga tattttagag acaatgttta ctgacgaaga aggtaaattg ggtgaaactg
    16261 aaaacacgca accagcttta ttgacgcata gttcggcatt attagcagca ctaaaaaatt
    16321 tgaatcccga ttttactatg gggcatagtt taggtgaata ttcaagttta gttgcagctg
    16381 acgtattatc atttgaagat gcagttaaaa ttgttagaaa acgtggtcaa ttaatggcgc
    16441 aagcatttcc ttctggtgta ggaagcatgg ctgcagtatt gggcttagat tttgataaag
    16501 ttgatgaaat ttgtaagtca ttatcatctg atgacaaaat aattgaacca gcaaacatta
    16561 attgcccagg tcaaattgtt gtttcaggtc acaaagcttt aattgatgag ctagtagaaa
    16621 aaggtaaatc attaggtgca aagcgtgtca tgcctttagc agtatctggc ccattccatt
    16681 catcgctaat gaaagtgatt gaagaagatt tttcaagtta tattaatcaa tttgaatggc
    16741 gtgatgctaa gtttcctgta gttcaaaatg taaatgcgca aggtgaaact gacaaagaag
    16801 taattaaatc taatatggtc aaacaattat attcaccagt acaattcatt aactcaacag
    16861 aatggctaat agaccaaggt gttgatcatt ttattgaaat tggtcctgga aaagttttat
    16921 ctggcttaat taaaaaaata aatagagatg ttaagttaac atcaattcaa actttagaag
    16981 atgtgaaagg atggaatgaa aatgactaag agtgctttag taacaggtgc atcaagagga
    17041 attggacgta gtattgcgtt acaattagca gaagaaggat ataatgtagc agtaaactat
    17101 gcaggcagca aagagaaagc tgaagcagta gtcgaagaaa tcaaagctaa aggtgttgac
    17161 agttttgcga ttcaagcaaa tgttgccgat gctgatgaag ttaaagcaat gattaaagaa
    17221 gtagttagcc aatttggttc tttagatgtt ttagtaaata atgcaggtat tactcgcgat
    17281 aatttattaa tgcgtatgaa agaacaagag tgggatgatg ttattgacac aaacttaaaa
    17341 ggtgtattta actgtattca aaaagcaaca ccacaaatgt taagacaacg tagtggtgct
    17401 atcattaatt tatcaagtgt tgttggagca gtaggtaatc cgggacaagc aaactatgtt
    17461 gcaacaaaag caggtgttat tggtttaact aaatctgcgg cgcgtgaatt agcatctcgt
    17521 ggtatcactg taaatgcagt tgcacctggt tttattgttt ctgatatgac agatgcttta
    17581 agtgatgagc ttaaagaaca aatgttgact caaattccgt tagcacgttt tggtcaagac
    17641 acagatattg ctaatacagt agcgttctta gcatcagaca aagcaaaata tattacaggt
    17701 caaacaatcc atgtaaatgg tggaatgtac atgtaatata tttgagctaa agctcattga
    17761 cgcagtggtt gactggtcat ccaatggaga aatgcctgac ctagtcaact ttgcggggga
    17821 aattctaagc aaccaagata tggttccaga atttctccct aagaaacagt aatcaataaa
    17881 ttgataacta ataagcggaa cgcctgagct aaagctcatg cataagaaac agtaatcaat
    17941 aaattgataa ctgtttctaa aatttctact tgttttttag aatttaaaat gggaaaatat
    18001 aatagtctat gtataggcat ttttaaagga ggtgaatcga cgtggaaaat ttcgataaag
    18061 taaaagatat catcgttgac cgtttaggtg tagacgctga taaagtaact gaagatgcat
    18121 ctttcaaaga tgatttaggc gctgactcac ttgatatcgc tgaattagta atggaattag
    18181 aagacgagtt tggtactgaa attcctgatg aagaagctga aaaaatcaac actgttggtg
    18241 atgctgttaa atttattaac agtcttgaaa aataataaat cttacatctg ggtcgtcagt
    18301 attgtcgact cagttttttt ctttaattat caatagtttt aacgtaaaat taaagatgat
    18361 tcaagagcaa cacataaagg agataaaata atgtctaaac aaaagaaaag tgagatagtt
    18421 aatcgtttta gaaagcgctt tgatactaaa atgacagagt taggctttac ttatcaaaat
    18481 attgatttat accaacaagc attttcgcat tcgagtttta ttaatgattt taatatgaat
    18541 cgtttagacc ataatgagcg tttagagttt ttgggtgatg cggtattaga attgacggtt
    18601 tcacgatatt tatttgataa acatcccaac ttgccagaag ggaatttaac aaaaatgcgt
    18661 gccactattg tatgtgagcc ctcacttgta atatttgcga ataaaattgg attgaacgaa
    18721 atgattttac ttggtaaagg tgaagagaaa acagggggac gtacaagacc atcattaata
    18781 tcagacgcat tcgaagcatt tattggggca ttgtatttgg atcaaggact agatatagtt
    18841 tggaaatttg ctgagaaagt cattttccca catgtagaac aaaatgagtt attaggcgtg
    18901 gtagatttta aaacacaatt ccaagaatat gtgcaccagc aaaataaagg tgatgtaacc
    18961 tataatttaa taaaagaaga gggaccggca catcatcgtc tattcacttc agaagttatt
    19021 ctgcaagggg aagcaatagc tgaaggtaaa gggaaaacga aaaaagaatc agaacaacgc
    19081 gctgctgaaa gtgcctataa gcaattaaag caaattaaat agaaatttat atgatacgac
    19141 atataggtgt tcgatttagg atttatggaa gaatttttaa tattttatgg aacaacttcg
    19201 aaatttaata tacaaatact aaaatatcgt atacatgtgt tctttaaaat tgtgataagg
    19261 agtttaggat ggtttattta aaatcaatag atgccattgg atttaagtct tttgcagatc
    19321 aaaccaatgt tcaattcgat aaaggtgtga ctgcaattgt tggtccaaat ggaagcggta
    19381 aaagtaatat tacagatgct attaaatggg tgttgggtga acaatcggct aaatcattac
    19441 gtggctcaaa aatggaagat attatcttct caggtgcaga acatcgcaaa gctcaaaatt
    19501 atgctgaagt acagttaaga ttagataatc attctaaaaa gctcagtgtt gatgaaaacg
    19561 aagttattgt aacaagaaga ttgtatcgaa gtggtgaaag tgagtactac ataaataatg
    19621 accgtgcaag attaaaagat attgccgatt tatttttaga ttctggattg gggaaagaag
    19681 catatagcat tatctcgcaa ggtagagttg atgaaatact aaatgctaaa ccaattgata
    19741 gacgtcaaat tattgaagaa tcggctggtg tacttaaata taaaaaacgt aaagctgaat
    19801 cattaaataa acttgaccaa acagaagata atttaacgag agtagaagac attttatatg
    19861 atttggaagg tcgcgtagaa cctctaaaag aggaggcagc tatagctaaa gaatataaga
    19921 cactttcaca ccaaatgaaa catagtgaca ttgtagttac agtgaacgat attgatcaat
    19981 atacaaatga caatagacaa ttagatcaac gtttaaatga tttacaaggt caacaagcaa
    20041 ataaagaagc tgacaagcaa cgtttaagcc aacaaattca acaatataaa ggtaagcgtc
    20101 atcaacttga taatgatgtt gaatcgctta attatcaatt agtaaaagct acggaagcct
    20161 ttgaaaaata tacgggacaa ttaaatgttt tagtagaacg taagaaaaat caatctgaaa
    20221 caaatgcacg atatgaagaa gaacaagaaa atttaattga gcttttagaa aatatatcaa
    20281 gtgagatttc tgaagctcaa gatacttata agtctctgaa aagtaaacaa aaagaactca
    20341 atgctgtcat tcgtgaactt gaagaacaac tatatgtttc agacgaagca cacgatgaaa
    20401 aattggaaga aattaaaaac gaatactata cattaatgtc agagcaatca gatgttaaca
    20461 atgatattcg ttttttaaag catactatag aagagaatga ggttaaaaaa tcaagactag
    20521 attctcgatt agttgaagtc tttgagcaat tgaaagatat tcagggtcaa ataaaaacga
    20581 ctaaaaaaga atatcaacag accaacaaag aactttctgc tgtagataaa gaaattaaaa
    20641 atatagaaaa agacctcact gatacaaaaa aagcacaaaa cgaatacgaa gagaaattgt
    20701 atcaagcata tcgatatacc gaaaaaatga aaacacgtat tgatagtttg gcaacgcaag
    20761 aggaagaata tacttatttt ttcaatggcg tcaaacatat tttgaaagct aaaaataaag
    20821 aattaaaggg tattcatggt gcagttgcgg aaattattga tgtgccatct aaattaactc
    20881 aggcaattga aacagcatta ggtgcttcat tacaacatgt cattgtagat tcagaaaaag
    20941 atggacgcca ggctattcaa tttttaaaag aacgtaattt aggtcgtgcg acgtttttac
    21001 cattaaatgt tatacagagt agagcggtag cgactgatat taagtctatt gctaaagagg
    21061 caaacggatt tattagtatt gcttcggaag cagttaaagt agcaccagaa tatcaaaata
    21121 ttatcgggaa tttattaggt aatacgatta tcgttgatca tttaaagcat gcaaatgaat
    21181 tggcacgtgc gattaaatat cgaactcgta ttgttacttt ggaaggtgat attgtaaatc
    21241 ctggtggttc tatgactggt ggtggcgctc gtaagtcaaa aagtattctg tctcaaaaag
    21301 acgagttgac aacaatgaga caccaattag aagattactt gcgtcaaaca gaatcatttg
    21361 aacaacaatt taaagagttg aagataaaaa gtgatcaatt aagtgaactg tattttgaaa
    21421 aaagtcaaaa gcataataca cttaaagagc aagtgcatca ttttgaaatg gagctcgata
    21481 gattaactac acaagaaaca caaataaaaa atgatcatga agaatttgaa tttgaaaaaa
    21541 atgatggtta tacgagtgac aaaagtcgac aaactttgag tgaaaaagaa actcatttag
    21601 aaagtattaa agcatcttta aaacgactag aagatgaaat tgaacgctac acaaaacttt
    21661 ctaaagaagg taaggaaagc ttaactaaaa cacaacaaac gttacatcag aaacaatctg
    21721 atcttgctgt ggttaaagag cgtattaaaa cacaacaaca gacaatagat cgattaaata
    21781 atcaaagtca acaaaccaaa catcaattaa atgatgttaa agaaaaaata gcattcttta
    21841 attcggatga agtgatgggc gaacaagctt ttcaaaatat taaagatcaa attaatggtc
    21901 aacaagaaac gagaacacgc ttatctgatg aattagataa attgaaacaa caacgtattg
    21961 agttgaatga acaaatcgat gcgcaagaag ctacactaca agtttgtcac caagatattt
    22021 tagctatcga aaatcactac caagatatta aagctgaaca atcaaagcta gatgtattaa
    22081 ttcatcatgc gatagatcat ttaaatgatg aatatcaatt gactgttgaa cgtgcaaaat
    22141 ctgaatatac gagtgatgaa tcgattgacg cattacgtaa aaaagttaaa ttaatgaaga
    22201 tgtcgattga tgaactaggt cctgtaaact taaatgcaat tgaacaattt gaagagttaa
    22261 atgaacgtta tacattttta agtgagcaac gtacagatct tcgtaaagct aaagaaacat
    22321 tagagcaaat tataagtgaa atggatcaag aggttactga aagatttaaa gaaactttcc
    22381 atgctattca aggacatttt acagctatgt tcaaacaatt gtttggtgga ggcgatgcag
    22441 aattgcaatt aactgaagcc gattatttaa cagctggtat tgatattgtg gtacaaccac
    22501 cgggtaaaaa gttgcaacat ttatcgttac taagtggtgg tgagcgtgca ttaactgcta
    22561 ttgctttact atttgcaatt ttaaaagtaa gatctgcacc ttttgttata ttagatgagg
    22621 ttgaagctgc attagatgaa gcgaatgtta ttagatacgc aaaatattta aatgagttat
    22681 cagacgaaac acaattcatt gttattacac accgtaaagg aacaatggaa tttgcagata
    22741 ggttatacgg tgtaacaatg caagaatcag gtgttactaa acttgtgagt gtgaatttaa
    22801 atacaataga tgatgtgttg aaggaggagc aataatgagc ttttttaaac gcttaaaaga
    22861 taagtttgca acaaataaag aaaatgatga agttaaatcc ttaacagaag aacaaggtca
    22921 agacaaatta gaagatacac attctgaagg ttcaacgcag gacgtaaatg atttagcaga
    22981 aaatgctgaa gtgaaaaaga agccacgcaa gttgagtgaa gcggattttg atgacgatgg
    23041 cttaatatca attgaagatt ttgaagaaat tgaagctcaa aaaatgggtg ctaaatttaa
    23101 agcaggactc gaaaaatctc gtcaaaattt ccaagaacaa ttaaataatt tgatatcgag
    23161 atatcgtaaa gtagatgaag acttttttga agctttagaa gaaatgttaa tcactgcaga
    23221 cgtcggtttt aatacagtga tgacgttaac tgaagaatta cgtatggaag cacaacgacg
    23281 taatattcaa gatactgaag atttgcgtga agtcattgtt gaaaaaatcg tagagattta
    23341 ccatcaagaa gatgataatt cagaagctat gaacttagaa gatggtcgtt taaatgtcat
    23401 tttaatggtt ggtgtgaatg gtgttggtaa aacaacaaca attggaaaat tagcttaccg
    23461 atataaaatg gaaggtaaaa aagtaatgtt agctgcgggc gatactttta gagcgggtgc
    23521 tattgatcaa ttgaaagttt ggggcgaacg tgttggtgta gatgtaatta gccaaagtga
    23581 aggttctgat ccagctgctg ttatgtatga tgcgattaat gccgctaaaa acaaaggtgt
    23641 tgatatttta atctgtgata ccgctggacg tttacaaaat aaaacaaatc taatgcaaga
    23701 attagaaaaa gttaagcgtg taattaatcg agcagtgcca gatgcgcctc atgaagcatt
    23761 actatgttta gatgctacaa ctggtcagaa tgcgttgtca caagctagaa actttaaaga
    23821 agtaacaaat gttacaggta ttgtattaac gaaattagat ggtacagcca aaggtggtat
    23881 cgtattagcc attcgtaatg aattgcacat tccagttaaa tatgtaggtt taggtgagca
    23941 attagatgac ttacaaccat ttaaccctga aagttatgtc tacggcttat tcgctgatat
    24001 gattgaacaa aatgaagaaa taacaacagt tgaaaatgat caaattgtaa cagaagaaaa
    24061 ggacgataat catggggcaa aatgatttag ttaaaacgtt acgaatgaat tatttgtttg
    24121 atttttatca atccttattg acgaataaac aacgtaatta tttggaatta ttttatctcg
    24181 aagattattc tttaagtgaa atcgcagata cttttaatgt gagtagacaa gcagtttatg
    24241 ataatataag aagaactggc gatttagttg aagattatga aaagaaattg gaattatacc
    24301 agaaatttga gcaacgccga gaaatatatg atgaaatgaa aaaacattta agtaatccag
    24361 aacaaataca acgttatatt caacaattag aagacttaga atagatataa attgagggag
    24421 gacatcgata tggcatttga aggcttatca gaacgcctgc aagcgacgat gcaaaaaatg
    24481 cgtggtaagg gtaaacttac tgaagctgat ataaagataa tgatgcgtga agtaagatta
    24541 gcgttacttg aggctgacgt aaactttaaa gtggtaaaag aatttattaa aacagtatca
    24601 gaacgcgcat taggttccga tgtaatgcaa tcattaacac cagggcaaca agttattaaa
    24661 atagttcaag atgaattaac gaagttgatg ggtggagaaa atacatcgat taatatgtca
    24721 aataaaccac ctactgttgt tatgatggtt ggtttacaag gtgctggtaa aacaacaact
    24781 gcaggtaaat tagcattatt gatgcgtaaa aaatacaaca aaaaaccaat gttagttgca
    24841 gcagatattt atcgtccagc agcgataaat caattacaaa cagtaggaaa acaaattgat
    24901 attcctgtat acagtgaagg ggatcaagta aagccacaac aaattgtaac taatgcatta
    24961 aaacatgcta aagaagaaca tttagacttt gtaatcattg atacagcagg tcgattacac
    25021 atcgatgaag cattgatgaa cgaattaaaa gaagtaaaag acattgctaa accaaacgaa
    25081 attatgttag ttgtcgattc aatgacgggt caagatgctg tcaatgttgc agaatcattt
    25141 gacgatcaac ttgatgtcac aggtgttacc ttaactaaat tagatggtga tacacgtggt
    25201 ggtgcagctt tatctattcg ttcggtgaca caaaaaccaa ttaaatttgt tggtatgagt
    25261 gaaaagttag atggtttaga gctattccat cctgaacgta tggcatcacg tattttaggt
    25321 atgggtgatg tgttaagttt aattgaaaaa gcacaacaag atgtggatca agaaaaagca
    25381 aaagatttag agaaaaagat gcgtgagtca tcatttactt tagatgattt tttagaacaa
    25441 cttgatcagg tgaaaaattt aggaccactg gatgatatta tgaaaatgat tccaggtatg
    25501 aataaaatga aagggctaga taagcttaat atgagtgaaa agcaaattga tcatattaaa
    25561 gcgattatcc agtcaatgac gccggctgaa agaaacaatc cagacacatt gaatgtatca
    25621 cgtaaaaagc gtattgctaa aggttctgga cgttcattac aagaagtcaa tcgtttgatg
    25681 aaacaattta acgatatgaa gaaaatgatg aaacaattca ctggtggcgg taaaggtaaa
    25741 aaaggtaaac gcaatcaaat gcaaaatatg ttaaaaggta tgaacttacc attttaatgt
    25801 ttaatttaac atgctgattt ttgttgcaat gagatgtgca tcccgaaagt tatactaaag
    25861 aatataactt tatggggtgc atatttttag atacacaata ataattaaat actgaaattg
    25921 ctgatttgct agaacatgag gttattgttg ctaatattaa taagttatat tgggagaacg
    25981 tgtgcggtag aagtggtacc attcatgtcc ttacgggata aattaactaa acacgtatgt
    26041 ggtgaaggct actatgaatt tttataacct attaacagtc taatttagta atcacatagt
    26101 tatttttgta aggaaaaggt tcaataattt tctaggtaaa gaaaaaactc tttacaaaca
    26161 ttcacacacc tgttaatatt atttcttgta gaaaataaaa attaaaacat gacttaaagg
    26221 agattttata aatggcagtt aaaattcgtt taacacgttt aggttcaaaa agaaatccat
    26281 tctatcgtat cgtagtagca gatgctcgtt ctccacgtga cggacgtatc atcgaacaaa
    26341 tcggtactta taacccaacg agcgctaatg ctccagaaat taaagttgac gaagcgttag
    26401 ctttaaaatg gttaaatgat ggtgcgaaac caactgatac agttcacaat atcttatcaa
    26461 aagaaggtat tatgaaaaaa tttgacgaac aaaagaaagc taagtaattt agcgtaaaat
    26521 tgttctaaca ataagaataa ctcgtttaca ctgacagtta ttactcaatg atacgttggg
    26581 aatatcacat gttagtaata tagaacgtat tgggtaccat aatggtgccc tttttctttg
    26641 aattattttc aattaaaata gaagtgttca aagcatagag ttggaggtaa tagaatgaga
    26701 gttgaagttg gtcaaattgt taacacacat ggtattaaag gtgaaattaa agtaaaatcc
    26761 aattcagact ttacagacgt tcgttttcaa cccggtcaag tgctgacagt tgtgcataac
    26821 aataacgacc ttgaatatac tgttaagtca catagagtgc ataaagggct tcatatgctc
    26881 acatttgaag gtattaataa tattaatgat attgagcatt taaaagggag ttctatttat
    26941 caagagcgtg atcatgaaga tatcgtactt gaggaaaatg aattttatta ttcagatatt
    27001 ataggatgta cagtttttga tgatcaagaa acaccaatag gtcgtgtaat taatatattt
    27061 gaaacaggtg cgaatgatgt gtgggtgatt aaaggatcta aagaatattt gattccttat
    27121 attgctgatg ttgtaaaaga agtggatgtt gaaaataaaa aaattatcat cacgccaatg
    27181 gaaggattgt tggattaatg aaaattgatt atttaacttt atttcctgaa atgtttgatg
    27241 gtgttttaaa tcattcaatt atgaaacgtg cccaagaaaa caataaatta caaatcaata
    27301 cggttaattt tagagattat gcaattaaca agcacaacca agtagatgat tatccgtatg
    27361 gtggcggaca aggtatggtg ttaaagcctg aacctgtttt taatgcgatg gaagacttag
    27421 atgtcacaga acaagcacgc gttattttaa tgtgtccaca aggcgagcca ttttcacatc
    27481 agaaagctgt tgaattaagc aaggctgacc acatcgtttt catatgcgga cattatgaag
    27541 gttacgatga acgtatccga acacatcttg tcacagatga aatatcaatg ggtgactatg
    27601 ttttaacagg tggagaattg ccagcgatga ccatgactga tgctattgtt agactgattc
    27661 caggtgtttt aggtaatgaa cagtcacatc aagatgattc attttctgat gggttattag
    27721 agtttccgca atatacacgt ccgcgtgaat ttaagggtct aacagttcca gatgttttat
    27781 tgtctggaaa tcatgccaat attgatgcat ggagacatga gcaaaagttg atccgcacat
    27841 ataataaaag acctgactta attgaaaaat atccattaac taatgcagat aagcaaatat
    27901 tagaaagata taaaatagga ttgaaaaaag gttagtattg tgctaacata atctgagtgt
    27961 ggaaacacaa aaatcactat gatccgctgc tatatatttg tcgaggcaag aacataggtt
    28021 gaagaggaga atttataatg acaaatcaca aattaatcga agcagtaact aaatcacaat
    28081 tacgtacaga cttaccaagt ttccgtcctg gtgatacttt acgtgtacac gtacgtatca
    28141 ttgagggtac tcgtgagcgt atccaagtat tcgaaggcgt tgtaattaaa cgtcgtggcg
    28201 gtggcgtttc tgaaacgttt acagttcgta aaatttcatc aggtgttggc gtggaacgta
    28261 cattcccatt acacacacca aaaattgaaa aaatcgaagt taaacgtcgt ggtaaagtac
    28321 gtcgtgctaa attatattac ttacgtagtt tacgtggtaa agctgctaga atccaagaaa
    28381 ttcgttaatc agcatttaaa caaagctatg tatgagtcaa attcgactca atcaataaag
    28441 ccatctaggt cacttttatg gagtgatata gatggctttt tttgctgttt tattaatgta
    28501 aagttgataa tgtaattgtt tttcccttct ttattcataa cgtgtttgct caaaatggtt
    28561 atataaacaa aaatctacta agtgccatga aaaaggactt agtagattta ttaaatatgt
    28621 gcgtagcaac attatttttg tcttgcccat ctcgtgaaaa taatactaca tataatgcca
    28681 aatatagtaa ttgttataag taaataatag tatggtgggg tatagctcaa ttgaatcttt
    28741 gttatatttt taggtgcttt aatgccggtc ataacaccat ttacttgttc aactttaaga
    28801 ctttgatcac ctgatgtcgc tttcatacct tgattatatg ctgttggcaa aacaatatac
    28861 ccagatgaat ttttattttt agtaatagta taaccttgct ttgtctttct aactttgaca
    28921 gcctctaatg aatttgaagc gtctttaagc gtggtataat cttcgccgta tatccctttt
    28981 aaatttacac gatacttacc tttaggcaat gataatctaa ttctatctga agctttaatg
    29041 cgtatcgtta cgggtgttac aaagcgtcga tatttataag tgagtttatt tctttcttgt
    29101 gtatattcat tcactttaac atcatgagct ttatccggcg aaagtaattc taaatccatt
    29161 tcaaaataca aatctttaaa ttgattagaa actgattttg gcaactgtac agttagacca
    29221 ccattatttt gtttaacttg taataaatgt tttgtaggag attgccaggc tgcactattt
    29281 aatttaattg ttgaatctga tagtaaattt ttattggctt taaaatgtgt attaacatct
    29341 ttagtattgt tagaaacaat cccttgcaac attgcttgtt ctttatctaa tggagatttt
    29401 aattctttat tggaaaagac cttatttgta atatgtgcac ttggataatg gatggtattt
    29461 ttagaatgaa tccaacgaac tttattgtct ttgtgttcag agttaatttt aaatccatat
    29521 ggtaagttgt catcatgatt cactctaatt cgatcattaa cattccaaag tgatagtaaa
    29581 ttttgacgat tgccaagtag tctataagtg ctgtttttat cgattggcat attaatttgg
    29641 agtgtcttgt cataatattt taaaatgtct ccattaaaaa tactagaata taatgaaatg
    29701 ccattataat gatatataaa tggtgaattt aatgcatagt ctgacatata atcaatgcga
    29761 ttaaatgggc ctgttgcatt tttatttatc ttttttataa gctggtttac atagttacta
    29821 tggtaatcat gttgtttcaa cgatgataat gattgttgat aaggtttgat tgccatattt
    29881 ttgttgttat ctaaaatgac gatttgttga atcataacga ttaatactaa tattgcaacg
    29941 gttaattttt tataacgcca taaactaaat tttaaaataa cagcaagcac catttgcagg
    30001 ataataccta ctataagtgc gagtgggtgt gtcggtgata gtaatacata aagtaatgcg
    30061 atgatgctta ctggtattgt tctgattaaa taatatttca tatttaatgt tgataaatgt
    30121 tgaataaaca atccgcaaag agcacttgat gatagtgcta agatatacac ccaacgcctt
    30181 tctggaaatg aaaaaccatt aaaagcactg tcaaaatact gtgataatga accaataaat
    30241 aatacccatg ttactattgc gaaaagtcta taaaagtaaa aacgatacag tttgaatgac
    30301 aataatgcaa cgatagtgag aattgaaatc gtaatgaaaa atccatcgct aaagaaaaaa
    30361 taatgataat caagtggtgt caaaaacggt atatcaacat tgggattttg ctttctgtca
    30421 ttttccaaaa acgcagaaat gccagtgaat aaaccaaata cacttgataa cacactcaaa
    30481 actgtagcag atatgacgca aattaatttt tgtgttctag agacaatgtc atatttgtaa
    30541 gtgaaaatga gtcgatataa atagtagcaa cctataataa tagcttgata ataactgaaa
    30601 taaaaattgc taaatagtgt taaggctatc gcaacaatga aaataccgat tttgcgttgt
    30661 tgaaaatatc tttccaaacc aagaatcgat aatggcaata aatataataa atttccataa
    30721 aatgaccaag taaaattaaa gtatataacg acagttgaca tgccgtataa aatcgtagcg
    30781 atcatatttg ttgagcgttt aaagtgtaat attttaaata agtagaaggt cacgacaaat
    30841 gttatgatag ctcgtaccat agccataata agttggtttg tcggccaaaa atgtattgtt
    30901 gtcggattaa atataccaac agtttctcct attttaatga atagaaaatt gagccacatt
    30961 aaaggtgaca gcgaataata atatgatagt cctttcatat aatcgccacc taatccaaac
    31021 gatgcatcat ataaactaga aaaactactt agatgttcat acaaatacat ttgaaatggc
    31081 atcatttgac ggaatccatc tccagatcca ctaaaaacag taccattcac aatatattca
    31141 tagatatgag tagaaaataa aataagcgtt aatattacgc tcatgaaagt tatgaccaag
    31201 aattgtttga cgtttgaatt taaccatttt tttaacacaa cattatcctc aactttcaaa
    31261 tttaaaatta agtttaactg aaactaaagt taatgaggtt ctcgataggt aaagacaaag
    31321 atgactgtgg aacagatacc ttatcatagt tacttaaact ttggattatt tttagtttat
    31381 cattaaacaa atatattgaa taataaaaat gtcatactgt attaattgaa tatcacctaa
    31441 taagcatctt agatttaata aatgataatt tttaattaga gaaaacttgg aataatcact
    31501 tataccttaa tctaaagcat tgttaagaag tgtgacaatg ttaaaataaa tatagttgaa
    31561 ttaatgaatt tgttctataa ttaacaagtt atagatttta ataatgagaa aagaattgac
    31621 gaaagtaagg tgaattgaat
//
LOCUS       DATTQF010000020        28512 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.020, whole genome shotgun sequence.
ACCESSION   DATTQF010000020 DATTQF010000000
VERSION     DATTQF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.020"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..99)
                     /locus_tag="VB241_000784"
     CDS             complement(<1..99)
                     /locus_tag="VB241_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEP1244104.1"
                     /translation="MLLCESKVINKNPKYRVIKYGDEYLMIDLVSTW"
     gene            complement(100..1494)
                     /locus_tag="VB241_000785"
     CDS             complement(100..1494)
                     /locus_tag="VB241_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1244105.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEQKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKDGKYLGKG
                     VAGVGFGIGMYDDMANDDKTVGEALSHNALTTGIGIGAGAGMTATIALFASNPVGWAV
                     IGGFVFGSVSTMLVDWAYQNNKAGIKDKADWVGHKLDDGIDVVKKSTEKAVDSVGEAT
                     KSISNHLNPMKWSW"
     gene            complement(1959..2060)
                     /locus_tag="VB241_000786"
     CDS             complement(1959..2060)
                     /locus_tag="VB241_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244106.1"
                     /translation="MCQYLCLKSVELGVIIMMIYYLKVVALTVRLRN"
     gene            2118..2558
                     /locus_tag="VB241_000787"
     CDS             2118..2558
                     /locus_tag="VB241_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEP1244107.1"
                     /translation="MNLEFNIAVHVLTFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLANDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNNIINFYKEKTIKDVIEDIQKEDLCHENI"
     gene            2545..3867
                     /locus_tag="VB241_000788"
     CDS             2545..3867
                     /locus_tag="VB241_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEP1244108.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NVGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDSNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDNYHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSSLYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTILRDNIYTHPTMAESFNDLFNF"
     gene            complement(3919..4260)
                     /locus_tag="VB241_000789"
     CDS             complement(3919..4260)
                     /locus_tag="VB241_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HEP1244109.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFAFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQERKL
                     KEKLEKRKAQQ"
     gene            complement(4437..5513)
                     /locus_tag="VB241_000790"
     CDS             complement(4437..5513)
                     /locus_tag="VB241_000790"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP1244110.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            complement(5526..6509)
                     /gene="mvaD"
                     /locus_tag="VB241_000791"
     CDS             complement(5526..6509)
                     /gene="mvaD"
                     /locus_tag="VB241_000791"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP1244111.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            complement(6514..7434)
                     /gene="mvk"
                     /locus_tag="VB241_000792"
     CDS             complement(6514..7434)
                     /gene="mvk"
                     /locus_tag="VB241_000792"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP1244112.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHKFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            complement(8021..8857)
                     /locus_tag="VB241_000793"
     CDS             complement(8021..8857)
                     /locus_tag="VB241_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP1244113.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENFDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(8860..9846)
                     /gene="pta"
                     /locus_tag="VB241_000794"
     CDS             complement(8860..9846)
                     /gene="pta"
                     /locus_tag="VB241_000794"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP1244114.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSFITAAQALQ"
     gene            10014..10766
                     /locus_tag="VB241_000795"
     CDS             10014..10766
                     /locus_tag="VB241_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HEP1244115.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFNQFFAIS"
     gene            11314..12078
                     /locus_tag="VB241_000796"
     CDS             11314..12078
                     /locus_tag="VB241_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HEP1244116.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLASIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            12091..12543
                     /locus_tag="VB241_000797"
     CDS             12091..12543
                     /locus_tag="VB241_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEP1244117.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(12685..14169)
                     /locus_tag="VB241_000798"
     CDS             complement(12685..14169)
                     /locus_tag="VB241_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP1244118.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLLTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVSVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(14290..14658)
                     /locus_tag="VB241_000799"
     CDS             complement(14290..14658)
                     /locus_tag="VB241_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEP1244119.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(14791..15171)
                     /locus_tag="VB241_000800"
     CDS             complement(14791..15171)
                     /locus_tag="VB241_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HEP1244120.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAPT
                     SNTPHIGEQQMNHRIANHNQMPHSQITQSTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(15172..15828)
                     /locus_tag="VB241_000801"
     CDS             complement(15172..15828)
                     /locus_tag="VB241_000801"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP1244121.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANTYLESVGKSPINWCESEA
                     "
     gene            16012..16842
                     /gene="thiD"
                     /locus_tag="VB241_000802"
     CDS             16012..16842
                     /gene="thiD"
                     /locus_tag="VB241_000802"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP1244122.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            17375..17542
                     /locus_tag="VB241_000803"
     CDS             17375..17542
                     /locus_tag="VB241_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HEP1244123.1"
                     /translation="MIIYRQYQHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(17743..18012)
                     /locus_tag="VB241_000804"
     CDS             complement(17743..18012)
                     /locus_tag="VB241_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244124.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLEAN
                     QCQINYVKPILINENIKFIEEHEQEIIAINDDGEIKIKISLSTKK"
     gene            complement(18015..18380)
                     /locus_tag="VB241_000805"
     CDS             complement(18015..18380)
                     /locus_tag="VB241_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HEP1244125.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYHCFNQPYSKEVPPLMCASL
                     WSKFDLFKKYANSELILTKSVINQTQKIKVDTIYVGHLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            complement(18355..19494)
                     /locus_tag="VB241_000806"
     CDS             complement(18355..19494)
                     /locus_tag="VB241_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEP1244126.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYGVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGELFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFDHGLLFKDGVTVGVDSN
                     LPGIGPVPAISNVLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKESMIASMGIGGGLGNAALFTRF"
     gene            complement(19496..20872)
                     /locus_tag="VB241_000807"
     CDS             complement(19496..20872)
                     /locus_tag="VB241_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP1244127.1"
                     /translation="MDVILEQLETHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTCLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPERLLNQCRKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICVKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQHDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLNKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            complement(21417..22817)
                     /locus_tag="VB241_000808"
     CDS             complement(21417..22817)
                     /locus_tag="VB241_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1244128.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVESADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIALLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFGLGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVAKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     VEHPKEALWWVKERKD"
     gene            complement(23320..23967)
                     /locus_tag="VB241_000809"
     CDS             complement(23320..23967)
                     /locus_tag="VB241_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1244129.1"
                     /translation="MKFENYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSAQDISH
                     YMGIPIEESFLKLADRPLDEATLAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(24080..24628)
                     /gene="hxlB"
                     /locus_tag="VB241_000810"
     CDS             complement(24080..24628)
                     /gene="hxlB"
                     /locus_tag="VB241_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HEP1244130.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEHGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVSEQTMQQNHANLE"
     gene            complement(24630..25262)
                     /gene="hxlA"
                     /locus_tag="VB241_000811"
     CDS             complement(24630..25262)
                     /gene="hxlA"
                     /locus_tag="VB241_000811"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HEP1244131.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(25339..26097)
                     /gene="nagB"
                     /locus_tag="VB241_000812"
     CDS             complement(25339..26097)
                     /gene="nagB"
                     /locus_tag="VB241_000812"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP1244132.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDAYDMNAEASTYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFENEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            26374..26736
                     /locus_tag="VB241_000813"
     CDS             26374..26736
                     /locus_tag="VB241_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HEP1244133.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEINEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            26750..27415
                     /gene="bshB2"
                     /locus_tag="VB241_000814"
     CDS             26750..27415
                     /gene="bshB2"
                     /locus_tag="VB241_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HEP1244134.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGTPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERIPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            27428..28306
                     /locus_tag="VB241_000815"
     CDS             27428..28306
                     /locus_tag="VB241_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEP1244135.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLCTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLEGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT         9134 a         5277 c         4282 g         9819 t
ORIGIN      
        1 ccaggtactt actaaatcaa tcattaaata ttcatcacca tatttaataa ctctatactt
       61 aggatttttg ttgataactt tagattcaca tagcaacatt taccagctcc atttcatagg
      121 gtttaaatga ttagaaatac tttttgtagc ttcaccaaca ctatctacgg ctttttctgt
      181 agatttttta acaacatcga taccatcgtc tagtttatgt cctacccagt ctgctttatc
      241 ttttatacca gccttattat tttgataagc ccaatctaca agcattgtcg aaaccgaacc
      301 aaacacaaag ccacctataa cagcccatcc tactggatta cttgcaaata acgcgatagt
      361 agcagtcata cccgcacctg cgccaatacc tattcctgta gttaaagcat tatgcgacaa
      421 tgcctctcca actgttttat catcgtttgc catatcatca tacataccta taccaaatcc
      481 tacacctgca acacctttgc ctaggtattt tccgtctttt gcaaatttac taccattttc
      541 tataaacaca ttacctgatg tacgtttgac ttccacaaat gaatttggac cggctgggcc
      601 tttcactcca cctgctgtat taataaatac accgaattta cttgcattta taccgccttg
      661 ctctaaaagt gttgacgtaa tatctaatcc tatatctcgt ttaatactat ctttattgtc
      721 atttatatat ttcattatac ttttcgggat atcgtcttct ggatgttctt tagcatatgc
      781 ctttataaca gcaaagtctg ctttatttaa agtttctttc tctgcttttt gttcaatttt
      841 ccccatagca actttcaaat atttttcatg acttgcttta gcccaatcaa gttctttacc
      901 tgaaggtata ttaaattgat ttgttgaaaa gttccaaaaa ttttgcgctt gggtaagtcc
      961 ttgttgtaca attttttgaa attcttcaac ttctttaaat atttctggtg atttttgatt
     1021 aaactcacgc aatttgcgta gcttctcttc taattcatgt ttttgttgac ctaatgttcg
     1081 tattatttgt tggttcgatg aaatggcttg ctgattatcg gaagcatgct ttttcaaatt
     1141 gttattcaaa ttttcatatc gcgtaatttg ttgacttaat gatctgatat cttcttcaag
     1201 ctctgattct tttaaagata tgctatcaac ctcactcgta taacgtgaca caaaattatc
     1261 gcaagcttgc ttcgttaaat cactcaatgt tttcatactt gttgataatg gaattaacac
     1321 cgtactaaaa aattgcttag ctgacgtata cgctttacct ttaagcgcat catcattgat
     1381 aaattgagta attgcttttt ccaacgcatc ataatttgaa ttcattgttt gactcaaatt
     1441 acccacactt gaagcttggt ttcgagatct gtctaaatac atgtcaatac tcatcggcat
     1501 gctccttttt caaaaatata tgattttcaa actatttaaa atcaaatgct ttttacatct
     1561 acaaaattgt aaaattttaa aactcggcga tgattatttc ttatgtaaag gagtctagat
     1621 gcaggtaaat tgagataaca tgtcgccttt tttcttattt tagcatatgg atataatggt
     1681 gtctttgtat attcgcaatt aataaataaa aattatcttt caatatttta attttattgc
     1741 gacaacatcc ttaacattaa atatattaat atctcaaaat atattcacta ttaaaatatg
     1801 tcatcagttg ttaaaagtat ttcctcatta tgcgaaatat caaaacgtct ctaaaatacg
     1861 aacaagttta tacaatcaca caacatcatc attcaaaatt ttattgccct tattttcaat
     1921 actcatttca tatctattag ttcatacata actaaaattc aattacgcaa tctcaccgtt
     1981 aatgcaacaa ctttcaaata ataaatcatc ataataatca cacctaattc aacacttttc
     2041 aaacataagt attgacacat tgagcaaaat gatttttaat tgtaactaat acagttacaa
     2101 ttacgaggtg agaaccattg aatttagaat ttaacattgc cgtacatgta ttaacttttt
     2161 taactaagca tcattcagaa aaattcaata gtagttcatt agctgaatta acttgtttaa
     2221 atcctgttca attacgccgc gtgacgactc aacttgtcga tttaaaaatg attaacacaa
     2281 tacggggtaa agatggtggt tatttagcaa atgatcgtag cgctgatgtc tcattagcaa
     2341 cattatataa acattttgtc ttagagaaag aacatcacac acgactattt actggcgacg
     2401 aaggtagtca ctgtcaaatt gctcgtaata ttgcaactac catgtcacat tatcaacaag
     2461 acgaacagaa taacattatt aatttttata aagaaaaaac aatcaaagat gtcattgaag
     2521 acattcaaaa ggaggattta tgtcatgaaa acatatgatt taattgtaat aggatttggg
     2581 aaagctggta aaactttagc gaaatacgct gcatcaacag gtcaacacgt cgcagttatc
     2641 gaacaatctt cgaaaatgta tggcggcact tgtatcaacg taggatgtat accttcgaag
     2701 acacttgtac atgatggatt agaaggcaaa tcctttgaag caagttataa ccgtaaaaac
     2761 gatgttgtca atgcgctaaa caataaaaat taccacttat tagcagacga cagtaacatt
     2821 gatgtattgg attttaaagc gcagtttaaa tctaatactg aagtgaattt attagatcag
     2881 catggtgata tcgtcgatag tattactgca cctcatatca ttattaatac cggtgctacc
     2941 tctatcattc ctaacataaa aggccttgat caagctaaac acgtcttcga ttcaacaggt
     3001 ttattaaaca ttagctatca acctaagcac ctcgttattg taggtggcgg ttatatcgcc
     3061 ttagaatttg cttcaatgtt tgcgaattta ggtagtaagg ttacagtatt agaacgtggt
     3121 gaatcattta tgccacgcga agatcaagat gtcgttgcac atgctattac tgacttagaa
     3181 aataaaggca ttgcattgca tacaaatgtt gaaacgactg aattgtcatc tgacaattat
     3241 catacaactg tccatactaa cgttggtaac tttgaggctg atgcagtact tttggctatc
     3301 ggacgcaaac cgaatacgga tttagcatta gaaaatactg atatcgaatt aggcgacaga
     3361 ggcgaaatta aagtcaatgc tcatcttcaa acaactgtgc cgcatattta tgctgcaggt
     3421 gatgttaaag gcggacttca atttacgtat atatctttag atgattatcg aattatcaaa
     3481 tcatctttat atggtaatca gtcacgtacg actgacaata gaggcagcgt gccttataca
     3541 gtatttatag atccaccatt atcacgtgtt ggattaacta gcaaagaagc tgccgctcaa
     3601 cattatgatt acactgagca tcaactttta gtaagtgcta tacctcgtca caaaattaac
     3661 aatgatccaa gaggtttatt taaagtagtc attaataatg aaaataatat gattttaggt
     3721 gctacattat atggtaagca atctgaagaa ttaattaata taattaaact tgcgattgat
     3781 caaaacattc catataccat attacgagat aatatttata cgcatcctac gatggccgaa
     3841 tcatttaatg atttatttaa tttctagaca aaacataaaa acctggtggc acgcattgaa
     3901 tgatgctgcc atcaggcttt attgttgtgc ttttcgcttt tctaattttt ctttaagctt
     3961 tctttcttgt tcttctttac gacgtttacg ttcttcatgt cgttttctta aacgctcttc
     4021 ttcttcagga tcacgtggtt tctttaattg ttgagaaact ttttcgatta attcttcttc
     4081 agtaagcgca gccagtgggc ggttattaac aaaagcgaat gtttttcggc gtccaggtcc
     4141 acaataagat tgacaaccta tcacgatttc agcatcggga tctaattttt ccaacttctt
     4201 ttgtaacgtt cttatattga ctgcctgaca ttcatcacaa ataaggaatg tatttttcat
     4261 attgctaccc acctttcttt atcatatcta tatcgtcgat ttcattaatt ttttcgttaa
     4321 ctctatctat tttactcttt ttaatatttt tttcaagata cgtaacacag ctggcaataa
     4381 aaaatggagc atttatcttc taattaaatt agatgattgc tcccctatca aatcatttat
     4441 tgcccatgat aaatattaaa ttttaatggt ttaataccat gttttgtcca ttcatcataa
     4501 attttttctt tatctacatc tttattgata attgtaatac cacagtctcc accaccagcg
     4561 cctgatgttt tagatgcgcc gtgatacttt tcagcaatat cacacaaata ttttagcttt
     4621 tcagtttcta tatcaactgt agcttcttta tccatacgtt gaataattgt acgattctga
     4681 cgcaccatct tttgcacacc tttaatgtta tttgttttaa aagcatgaat aagtttttca
     4741 acacaacgat gtgaatcttc taagaagtca ccgtaaaatg aaggatctga tttcaaacgt
     4801 ttcacttcgc taacaaagtg tggtgatgac gccggtgagc cagtccaacc gataagtact
     4861 tccatatttt caggtgcttg taatggttcg atgtgcaatc caggccagtt tttgattaaa
     4921 acttcttcaa ccgtagtatc ttcaatttga tgcttaaccc attcatgatc aaaagtacta
     4981 tacgctagcc atccactata tacactcaca gcaatatctc cgcatgaact taaactttgt
     5041 aacttcatat ttgcaatcac tgctagttta taaatgtata aattagataa cttcatatca
     5101 taaaattcat ttaatacttt tataactgat acaagtactg ctgcacttga acctaatcca
     5161 tatttatgac catttgaatc atctaaatta ctatcaatag tcagatgaaa atgcttcatc
     5221 gctatatcgc agcttttcgc atactgttca aatatttcaa tagctgtgac cacataattt
     5281 aattgttttg ctgcatgtgg atctgaaatg acaatactat cttcatctct actaaatgta
     5341 actgggttat gatgtaatgc ttttgaatga atggtacctt tatattggtc tgcttcttca
     5401 atagtagcag ttacaaaacg atctaacgca ataagtacag atttatatcc tggttctgtt
     5461 acagcatatt ctccagcaat ataaagtttt ccgggtgctt tgacctgaat cattttatct
     5521 cttccttact caattatttc aattcctgtg gcaataatgt cactatcaat aatttggtta
     5581 ttatcaaact gtgttaataa tttatctata atctgttgct tgtttttctt ttctacaagt
     5641 attttcacat taggtcccgc atccattgta aaataacacg gataccccgc ttctcggcat
     5701 tcgtgaacga gcgccattac atcataactt tcttgcacaa gatatgtgaa cggcggtgtt
     5761 gatcctagat tcgtggcatg catacgcaaa ccattttctt caattacttc accaaggcgt
     5821 ttaaaatctt tgtcttgaat cgctgctttt gcttcagcta aatcttcatc aatatgatct
     5881 aaccaatatt gataaaatct tgatgtgttt cgtgtcaacg acataccata tcgactaggt
     5941 acctttttag aatgttgatt aatcacaaca aatatcatgg caaggtcatc ttcaaaatga
     6001 ttcgattcaa gtggaacggc atatgacgtc tcatcattat accctttttc ccattctgca
     6061 aatccaccat aaatactacg cgacgcagaa cccgaaccga ttcgcgccaa tctcgataaa
     6121 tccttatctg acagctgcaa gtctagcgct tgattacaag ctgctgctaa agctgcatat
     6181 gcgcttgctg atgaagccaa tcctgctgct gttggtacaa aattgtcgct ttcaatttct
     6241 gcataccaat cgatgccagc tctatttctg acaatatcca tatattttga aattttctct
     6301 aattctttgc cactaacctt ttcaccattc aaccaaaatt gatcctgtgt taactggtcg
     6361 ttaaaagtga ctttcgtttc agtgtaaaat ttttctaatg taacagatat gctattattc
     6421 attggaatga ttagtgcttc atctttttta ccccaatatt taataagtgc aatattcgta
     6481 tgtgcacgtg ctttgccact tttaatcaac gcattaacct cctaaattct caatccatgt
     6541 atgtgctgca ccagcttttt ctacagcttt tacaatattt ttcgctgttg gtaaatcttt
     6601 ggcaagcaat aacatacttc caccacgacc agcaccagta agttttccag caatcgcacc
     6661 attttcttta ccaattttca ttaattgttc tattttatca tgactaactg tcaacgcctt
     6721 taaatccgca tgacattcat taaaaatatc cgctaaggct tcaaatttat gatgttcaat
     6781 cacatcactc gcacgtaaaa ctaacttacc gatatgtttt acatgtgaca tgtactgagg
     6841 gtcctcacaa agtttatgaa catcttctac tgcttgtctt gttgaacctt tcacaccagt
     6901 atcaataaca accatatagc cgtctaaact taacgttttc aacgtttcag catgaccttt
     6961 ttggaaccaa actggtttgc ctgatacaat cgtttgcgta tcaataccac ttggtttacc
     7021 atgtgcaatt tgctctgccc aattagcctt ttcaatgagt tcttctttcg ttaatgattt
     7081 ccctaaaaaa tcataactcg cacgaacaaa agcaaccgcg acagctgcac tcgatcctaa
     7141 cccacgtgat ggtggtaaat tcgtttggat cgttactgct agcggctctg taatattatt
     7201 taattctaca aaacggttca ccaaagactt aagatggtca ggcgcatcat ataacatacc
     7261 atcgtaaaca tcgcttttaa tagacgaata gttcccgctc tctaaggctt ctattaaaac
     7321 tttgatttta cctgcgttaa acggtactgc aatagcaggc tctccaaatg taacagcatg
     7381 ttctcctatt aaaataatct tacctgtcga ttccccatat ccttttcttg tcatgtcaat
     7441 atcacctttt atatttatcc tatacttgat acattgtttt tatttattag taaaagacat
     7501 catattctaa gttgcatacg cattcgcgtt aaatttcatt gcagtcttta tctcacatta
     7561 ttcatattat gtataaactt tattttaaat ttatatatga cttaacttga ttagtataaa
     7621 actaactttc gtttacttca aagtttaaat cttatcgagt gatatttcag attctttatc
     7681 tttttataaa atagccctac aatttataat tttccaccct aactataata ctacaaataa
     7741 taattggaat atatagattt actactaaag tattagaaca tttcaataga aggtcgtttc
     7801 tttcatagtc atacgcatta tatataccct attctcaatc tatttaatac gtaaaacatg
     7861 aaatttcctt attaaattta ttatttcaat catatcatta cttttaattt aatgatgttc
     7921 aatttaaata ttaggtcaat aacatattta tgctatttat ggatactttc aaaaataaca
     7981 gccccaaacg ataacttgaa aggggctgtt aaatatttaa ctattgcatt tggtctatca
     8041 ttttcttgtt tctttcaatc attttatcaa aatacgtatc gtatctttgc cattcttctt
     8101 gagtaattgg cgtcatattt aatacaccgc caagatcttt gattgcatat aatagtaaaa
     8161 acattacatc ttgaacagta atcgttttgt taagtaatgt ctctagtgag gccatacaag
     8221 atggttcaat ttcagggtat ttaaatttag tcacttcacc ttttaaagca tgttcataaa
     8281 atgtttgcat catcaatgca cgttctgaac cagagccttc aacacaaaga taaatttgta
     8341 cagcaatacc gcctctaact cttcgttgcg atatgcctgc aaatttctta ccatcgatac
     8401 ttaagtcaaa ttttcctggg caataagaat gttcaatttc catcgtatca atatcaaaat
     8461 tctcatcttc gaacattttg ctaattaaga gatacatcac agtaaacgct tcatcaatcg
     8521 ttgtttctgt ttgtcctttg aacatcagcg atatatttaa tacaccttga tctagaacga
     8581 cacctaggcc gccagaattt ctaacaatgg cattataacc aatctcattc gttaaataat
     8641 caatgccatc ttttaaaaac ggcaatcttg aatcatgaat accaagaata acagtatgtt
     8701 gatgaatcca agtacgcaca acattatctg atatatcttt gcccacactt tcacaaaatg
     8761 tatcatcgaa tgcgaaagat tgcataggct ctaatccaga agaatgatcg atatatcgcc
     8821 agttgacgcc attaaaatat ttactcgcta aatccatcgt tattgtaagg cttgcgctgc
     8881 tgtgatgaat gaaagattgt atacatcttc aattgagcag ccacgtgata agtcatttac
     8941 tggagaattt aaaccttgta atactggacc aactgcatca tatccaccta aacgttgtgc
     9001 aattttgtaa ccaatattac cagcttctaa acttgggaat acaaagacat ttgcatcacc
     9061 ttgtaattta gcacctggcg cttttttctc agcaacacct ggtacaatcg cagcatcaaa
     9121 ttggaattcg ccatcaatga ttgcttctaa tttttcttct tcagcttttt gttgtgctaa
     9181 tttgacagct tcttgaactt ttgtcacatc gtctgattta gcagaccctt ttgttgaaaa
     9241 gcttaacatt gcaacttttg gatccatgcc aaagcttaat gctgattttg cactttctac
     9301 tgcaatttct gcaagtcctt gtgaatcaag ttctggattg attgcacaat caccaaagat
     9361 gtactgttca tcacctttaa tcataaagaa gatacctgat gttcttgata cacctggttt
     9421 cgttttgatg atttgtaaag ctggacgcac agtgtcgcct gttgaatgtg ctgcaccact
     9481 aactaaacca tctgctttac cagcataaac aagcattgta ccgaagtagt tcacattgtt
     9541 taataattct tgtgcttgtt cttcagtcgc tttaccttta cgtcgttcaa caaatgattg
     9601 aactaattca gctttcaatt cacttgtcgc aggattaatt aattcaatat tagaaatatc
     9661 aagattaagt ttttgcgcta aagattgaac cttagtctca tcacctaaca cgattggtgt
     9721 aacataatct gttgcttgta attgtgttgc agctgttaga acacgctcgt cctctccttc
     9781 aggtaatacg attttaacgt ttttaccaga aagtttgtct tttaatacat ttaataaatc
     9841 agccataatg tcctcctgta atataaatct tattaatcat tcacggtata attatacgcc
     9901 attttatttc ataatgccac gtcaccaatt tgtaatcata attttatcgt tacccctttg
     9961 ctttatgata aaatttataa agaactgatg atttttgaaa aggagcgata aacatgagtc
    10021 aagcagccga aacattagat ggttggtata gtctacattt attttatgca gttgattggg
    10081 catcattacg tctagttcca aaggacgaac gcgatgcact tgtcactgaa tttcaatcat
    10141 ttttagaaaa tacagcaact gtaagatcat caaaatctgg tgatcaagct atttataata
    10201 taactggtca aaaagcagat ttgttattat ggttcttacg tcctgaaatg aagtctttaa
    10261 atcatattga aaatgaattt aacaaattgc gcattgctga cttcttaatc cctacatatt
    10321 catatgtatc agtcattgaa ttgagcaatt atttagctgg taaatctgat gaagatcctt
    10381 atgagaaccc tcatatcaaa gcaagattat acccagaatt accacattct gattatattt
    10441 gtttctatcc aatgaacaaa cgtcgtaatg aaacttataa ctggtacatg ttaactatgg
    10501 aagaacgcca aaaattaatg tatgaccatg gtatgattgg tagaaaatat gctggcaaaa
    10561 tcaaacaatt tattactggt tctgtaggtt ttgatgattt cgaatggggc gtaacattgt
    10621 tctcagacga cgtattacaa ttcaaaaaaa ttgtatacga aatgcgcttt gatgaaacaa
    10681 cagcacgata cggtgaattc ggtagtttct ttgtaggaca tattattaac acaaacgaat
    10741 tcaatcaatt ctttgcgatt tcttaataca ttggtacgtt tataaattaa taaaaaattc
    10801 caagcttatc ggtttaagct tggaattttt cgtttatctt cagtatattc ccgtatacat
    10861 aagacgtgat ttggtaaata gttgaaatct gtatgtttaa acttatatat atgtgctaat
    10921 gtaatatcaa taacaaagta cactttgctc atagcaaagt gtacccgagt agtcttcctt
    10981 gggagaactt taactactat cactacatat aaacgttaac ctcaatagaa attatacagt
    11041 cgctactcta tacaattttt gtaatggtta actaatatta ttttaaccta tttgaaatat
    11101 ttgaaacata tttttgtcga atttttttca atattttttc ctttttatac ttcaagagaa
    11161 ttttaactac taaaaattcc gatgattatt attacaatag tatcaaatat tagtttttta
    11221 aaatcaataa caacttatca aaaagctcat gtggttattt tatagtgtat aaactataat
    11281 gagtattaaa ttcttataaa caatggtgat gaaatggaca taaattcaga agaatacaaa
    11341 caagaggtac ttatcaaaga tgttgtcatg cttgctgctc gcatactatt agaatctggt
    11401 gcagaaggta cgcgtgtaga agataccatg acacgtattg caaaaaaact tggttacagt
    11461 gaaagtaaca gctttgttac aaacactgtc atccagttta cgttacattc ggaatcgttt
    11521 cctagaatat ttagaattac ctctcgagat acaaacttaa taaaaatttc tcaagctaat
    11581 aaaatttcgc gtcaaattac aaataatgaa atttctttag ccgaggcaaa aacacaactt
    11641 gaaaaaatat atgttgccaa gcgtgacagc agtcttccct ttaaaggttt tgctgcagca
    11701 atgattgcaa tgagtttctt atatttacaa ggtggtagat tgattgatgt tttaactgcg
    11761 atattagcag gtagtctagg atacctagtc actgagattt tagatcgtaa gttacacgca
    11821 cagtttatcc cagaattcat tggttcatta gttattggga ttatcgccgt tattggacat
    11881 acacttattc caacaggtga cttggcaagt attatcattg cggcagtcat gcctattgtt
    11941 cctggtgtat taataacaaa cgcaatacaa gatttatttg gtgggcacat gttgatgttc
    12001 acaacgaaat cattagaagc attggttact gcgtttggca tcggtgctgg cgttggtagc
    12061 gtattaattt tagtatagga gtatcagact atgttttgga tattaaactt tatctttagc
    12121 tttttagctt caatgttctt ctgcgtcatt tttgatgcac ctagaaaatt atatttatca
    12181 tgtggtttcg ttggtacgtg tggatggatg gtttacacct tattcttcaa cggctttaat
    12241 gtgcacacta tatactctag tttcttcggc agtcttgccc taggtttatt aagtcactat
    12301 atggctcgta aacaaaaaga acctgcaatc atttttatgg tgacaggtat catcccgtta
    12361 gtgccaggtg gattagctta cgatgcaacg aaaaatttag tattattaaa tttcagtaca
    12421 gcaatcaata ccatgctaga ggttacactt attgcaggag ctatcgcatt aggtttatta
    12481 ttcgccgacc aaatttccaa attaattgtt tctggattcg tgaaatcttt taaacgatta
    12541 taaatatttg ttagaaatgc ctcagcctta actcttggca attttatata aggttcaact
    12601 gcctcgtctt aaatgtcaat ttatgataca aaaaatcgca cgatacgcct tcaaattaag
    12661 aaggtatatt gtgcgattct tttattattt agttttttct ttttcaggtt cttcaatcat
    12721 atcatcaact tcattttcac ttctatttaa aatcaagcga cttaattcat cattatccat
    12781 atttttcaat ttaggatatc gaatcacaaa gaatattaaa ccgatgatta accatccaag
    12841 taatgcaata taagatggtg cagtcagtgc tgcaggagaa cctggcacta ataacaacgc
    12901 taagaaaatg aatgatacaa atgagccaat aatagcaaac gttttgtaaa ccggtgcata
    12961 cgtattactt tgtttgttat aactgaataa tttcgcagca gacaaacatg taataaagta
    13021 ggcaatggat acacctgtag atgacatatc tacgatccaa gtcaatgccg ttcttcctag
    13081 ccaaggtgca attaaagaca cacttactaa gaatatgatt gcgacatatg gtgttttgta
    13141 tttactatgt aatttactaa acattgttgg cattatacct gaacgtccca tagaaaataa
    13201 caagcgactt gaactcatca agaatccatt taaaccagta aatataccca tcataattgc
    13261 aattgctaat acacctaatc caatataacc aaatgctgtt tgtgtaacag cacctgttaa
    13321 ccacaattgt ccatttaaac tttgatgact tgttaataac caaccagtgt ataaaatcat
    13381 gacaacataa gttaatgatg ctgctaataa actgtacacg ataagcttaa atgtcttgtt
    13441 tggtgcaaag ttaaactctt ctgctgtttg tggaatatta tcaaatccaa catatgccca
    13501 tggtgccacg gatacaataa ccacaataga cactaaccat cctttgctag gttcagctaa
    13561 cggttgtaaa ttttcaagtg caaaattatt accaaagaat gaaccaaaga acatcaataa
    13621 tacgacgatt accatcgcca cacagaaata atattgtaat gatccagata cacttgcgcc
    13681 acgaatcgtt actagcatga atacaagtag taatacggtc gcaataatga tttccgtaat
    13741 ataaacgtcc cagcccgcaa tggtgtatag tttcccatta tttaagacat ctggcaataa
    13801 gaatttaact agtaaactga atgctgtcgc atttaaagca acaacacaga cataaccaaa
    13861 agttaaaaac catgatgaga agaaactcac atatctgccg aaacttaaga aactaaaggc
    13921 aaacgcgccc cctgatactg gaaatctctc tactaatgcg ccataactaa ccgcaattaa
    13981 tatcattaat aatgcaccaa taacaatacc aattgatgct gcaatcggac ctgactgctt
    14041 aatccagtct cctggtaaga tgaatgcgcc ccatccgata catgaaccat atgcaatcgc
    14101 ccatacaaac ttttcagata ggttttgttt taaatcgcct ctatctattt gcttattctt
    14161 tttttccata aataaaaact cacctcgaag gtattctata cccaaaagat gagtttgtaa
    14221 ctctttttct tgattatttt tattaaaaat ttaatgttta gttacatagg taatctaaag
    14281 ttttaaaatt taaccagcaa atttgaatgt cgcaatgatt aacattatcc atccaatgat
    14341 gaacaatacg ccaccaattg gcgtaatcgc acctaaaact ttaatttgag ttaatactaa
    14401 aatatataat gatccactaa agaaaataat accagcaaat attaaccagc cagcccagtt
    14461 aacattgatt gaagttgtac cactaattac acctataatt aataatgcta agccatggta
    14521 catttgatac gtcgttgctt tttcccatac tgataaatag tgatcactta tttttccttg
    14581 taaaccatgt gcaccaaatg cacctgttcc gacagccatc atcgcgttta atgcacctaa
    14641 aataataaat aatttcattg ctacctctcc ttatataatt cagtattaat aaatgtttaa
    14701 atccgaacac tcgaataaaa gtatatgcat cttgattatt aacctacata cttaacaagc
    14761 tattaagtaa ttatgtttca ttgttgcttt ttaaaaatca aatatggatt caccattgcc
    14821 aatgtcatca tctgtcgcta atctttgttg ttggtttgaa ggttgtgaat atgacttatt
    14881 atgatgatgt gctgaatgcg tatttacttt accacccatc gcttcaattt cagcagctgt
    14941 cacttgatgt gttgactgcg taatttgtga atgtggcatt tgattatggt tagcaatacg
    15001 atgattcatt tgttgttcac caatatgtgg tgtatttgat gttggagcat ataaagatgt
    15061 taatatatgt atggcataca tatgtttttc aaagtcatgg tcatgctgcg cctgctctgc
    15121 ttgtactaat tcttgctcaa ttcgtgctat taaagtttct ctattcacca tctacgcctc
    15181 actttcacac caattaattg gtgattttcc tactgactct aaataggtat tcgctttgga
    15241 atacggtttt gatccaaaga atcctctata tgcagacagt ggactaggat gcactgattt
    15301 tataatacaa tgtttagatg tatcgataag ctttattttt tgctgtgcag gtttccccca
    15361 caaaataaag acaacatgtt ctttataatc agacactgct ttaataattt catcagtaaa
    15421 tgtttcccaa ccaatatcac gatgagaatt tgcttcaccc tgtcttacgg ttaaaactgt
    15481 attcaataac aagacacctt ctcttgccca atcttgtaaa tgtggtgttt gtctaacaca
    15541 tccaatatca tctgctaatt ctttatacat attacgtaaa gatggaggga attttgcgtt
    15601 aggttgcact gaaaatgcta atccatgtgc ttggttcgga ccatgatacg ggtcttgtcc
    15661 taatataaca actttgatat tttcaaacgg tgttaaatca aacgcttgat aaatattttc
    15721 cctatcaggg tatacgattg cagtcgaata ttctttttct aaaaaatcat gcatagcttt
    15781 aaagtcatgt ttcgttgtta tgtcatgaaa aatttgcgac cattccattc aatctcacct
    15841 caggacatat tttaacatat cacacttatc taaagtgaat gacattttca aaagttacaa
    15901 tagtatgaat gattcatttc aacaatcatc aaatattgaa tgcttaatat ttttcaatta
    15961 ggttaaaaat gatatgatga atacaatatt attatgtagg agtgaagctc aatggcttta
    16021 aagaaagttt taacaattgc cggttctgac acaagtgctg gcgcaggtat gcaagcagat
    16081 ttgaaaacgt tccaagaatt agatacgtat ggcatggtcg ctttaactgc catcgttact
    16141 atggataaag atacatggtc acacgatgtc acaccattac ctatggacgt atttgaaaag
    16201 caacttgaaa cggctttatc aatcggacct gatgctatta aaacaggtat gttaggtact
    16261 gaggagataa tcaaacgtgc tggagaagta tatgaggcat ctaatgcaca atattttgta
    16321 gtagatcctg tcatggtttg taaaggcgaa gatgaagtgc ttaaccctgg aaatactgag
    16381 gcgatgatta aatatttact acctaaagca acggtagtga cgccaaactt atttgaagcg
    16441 ggtcaattat caggattagg taaattaaat tcaattgaag atatgaaaaa ggctgcaaca
    16501 attatctttg ataaaggcgc acagcatgtc atcattaaag gtggcaaagc tttagatcaa
    16561 gataaatcgt atgacttata ctatgatggt caaacatttt atcaactaac tacagacatg
    16621 ttccaacaaa gttataacca tggtgctggt tgtacatttg ctgcggcaac aactgcatat
    16681 ttagctaacg gtaagtcacc gaaagaagct gtgattagcg cgaaagcttt cgtcgcttct
    16741 gctattaaaa acggttggaa aatgaatgat tttgttggtc ctgtggatca cggtgcatac
    16801 aaccgtattg aacatatcga tgttgaagta acagaggttt aatgcttcgt tcataaaaaa
    16861 tagattaacc acaccttatg atttgattgt cattgctgtg cgcctgtaaa gttagctttt
    16921 tgagtctaac tttggggctc agtacacaat aaagtcactt tcggtgtggt tattttagtt
    16981 attaaatact tgtttgattt tcacagttaa attgtgcaaa taagttttgt aagaaatcgt
    17041 tatagatagt tttatttcaa catatgtata taatattcat ttacagtgtc acgaatcagt
    17101 tatcctaaaa aaacaaatcc tttcggaact ttattatgcc cttgtttaaa cataatgttt
    17161 tatcccgctc tactaattct gccatttttt gaaattaaaa ataacttctc aacgacttta
    17221 taaaaatata ttccttttaa aacatgatac ataaatagtc ttagtaaatt atacgttcta
    17281 tgtctgataa aaagttgata cttaaggttg atgtcacaca taaaaatgtt atttataata
    17341 taaccataga gtagcaaagg aggtaatata ggttatgatt atttatcgac agtatcaaca
    17401 tgaaggcgca ccagtttatg aaattataac caaaacgttt cagcatgttt caattaaatg
    17461 tgacgattca tttagtgata ctgaaatatt caaattgctc tctttattac aagacgatat
    17521 agatcatatg aaagttagtt aaattgacta atcgttccaa taatccagaa tttattaaac
    17581 attgatatag ttgttattat tattttagat taatgcatcc ccgtgcaaag aaagaaacgt
    17641 aacctatatt tatctctgtt cccaataata ataacaatac ataactgata ttagctgtcc
    17701 tttcatctcc taattaatat gcgttcatgc agctaatatc ggttattttt ttgtgctcaa
    17761 agaaatcttt attttaatct ctccatcgtc attaattgct ataatttctt gctcgtgttc
    17821 ttcaatgaat ttgatatttt catttattaa aataggttta acatagttaa tttgacactg
    17881 gttcgcctct aatgacatag ccatcaacat catttgactc actaattgtc ctggcacaat
    17941 ctcattatgt attggattat catcatttac gagcgccaaa tattcattta tccatatctc
    18001 attaaacttc atctctactt catcgcctta ataaaagttt gtgtaaccgt tatgacatgt
    18061 tgatcatttt tagttaatgt taaagccatt gtataacgtg tgatattgcg aatctgtcgg
    18121 cattcaatat cttctaaatg tcctacatat attgtgtcta cttttatctt ttgagtttga
    18181 ttaattactg attttgttaa aatcagttcg ctatttgcat attttttaaa taaatcaaat
    18241 tttgaccata atgacgcaca cattaatggt ggtacttctt tactataagg ttggttaaaa
    18301 caatgataat atgcagccac actatcctta gaaaatgaca catttaatcg ctggttagaa
    18361 tcgagtaaat aatgctgcat ttcctagacc tcccccaatc cccatagatg caatcatact
    18421 ctctttgtca aacatataaa ataatcgcgt cactaattgg gcaccgcttg caccgtatgg
    18481 atgacctgat gctaatgcac caccccatat atttaattgc gtatttgaaa tatttaaagc
    18541 ttgttggcag gcaaccacct gtgcactgaa cgcttcgtta atttcaatga cttcaatatt
    18601 ttctatcgtt aattgatttc tttttagtac gttggaaatg gctggtactg gaccaatgcc
    18661 aggtaaatta gaatcaacac ctaccgtaac accatcttta aataataaac catgatcgaa
    18721 acctaattcg tatgccatat ctttttccat aataagcaat agaactgcac catcattttt
    18781 catacaacta ttcgcagcgg taacggtccc acctttgatt acgggcttaa atcggccaaa
    18841 gttatctttc ggaatatgtg attttagact ttcatcagtg ttgaatagtt ctcctttaac
    18901 ggttataggt aatatttcct gagaaatatt tccattcttt acattttccg ctgtcagttg
    18961 atgacttcgg taagcaaatt catcttgtaa ttctcttgaa acaccataca tcttggctac
    19021 attttcagca ccttgaatca ttgatgggtc gctcatttca ggtgcaaatg atgcacgctc
    19081 ataaaactca ggtaatgcgg tttcgtacac agaatgcggt cgtttgattt tccaaggtgc
    19141 tcgacttgta ctttcaacac cgcctgcaat atataccttg ccagctccgg cttgaatcat
    19201 gcgacatgca tattgaacac tttcaagtcc agacccacat tgccgatcga ttgtgacgcc
    19261 aggtattgaa tctttgagcc ccgcttcaag caatgctttt cttgcaatat tgccaccatt
    19321 cccaacaaca ttacctaaaa ctacatcatc tattttagat attacctctg gatacttctc
    19381 tttaaaatgt tggaataaag gtttgagcaa ttgttctggc tctaaatgtt ttaaagtgcc
    19441 accatatttc ccaaatgcag ttcgtttagc tgcaactatg actgcttgat tcatattata
    19501 attcacctct caaatacatc gacatcattt tttctctagc aattttacca cttgcagtgt
    19561 aatacatctt ttctacatga tgaatcatcg atggaatttc atagcgtttc actttcttat
    19621 ttaaaaattg ttttacattt ttatgtgtaa gtgtcacatc accagaataa agcaatacac
    19681 ctatttgacc aaaacgctca tttggaatac cgatgataat tgcttcatca atgctcgacg
    19741 attgcgttaa aaggcgttca acatgtgctg gatatatatt ttgaccacca ataattaaca
    19801 tatcatgttg tcgtcccgtt aaatataaat actgctcttt tacatagcca ttatcattag
    19861 taacaaacca ttcatcatta tttatacatt gttcgcttac atagccacta aacatcatat
    19921 tactttttac acaaatagtt cctataccat tgtgatcgtg attcgttgtt ttcaattcca
    19981 catttggaaa tagcacacct acagattcaa ctggtgcttg ctgattcaag ttatagctga
    20041 taaaactggt ttccgatgta ccaaaaaatt caatcaaatt tatgtcattt gcttgatttt
    20101 ttatcttttt aaaaatagaa gaatgcagct tatccccact gctaaaaaat gattggattg
    20161 tatgttcatt gttataaact aacaataatg atttaatcat cgttggaaca agaaacatag
    20221 caactttgta tgatgatatt ttacgacatt gattaagtaa cctttcagga tgaaaagtgg
    20281 tctgtcctat aaaagtacga ccggaactta aagcaaacaa taacgcatat aatgttaacg
    20341 agtgcgatag tggtccgggg gctgctattg cattttcatt ttttaacatc aacatttcat
    20401 taacttcaaa agaagccaac catgaatctt catcacgata atatgctttt ggcagtccag
    20461 ttgtccctga agtaaaacca atatgtaata aattggggta gtgctgtaat tgcgttgaat
    20521 caataaacat aggtgagtct atattctgca tgagacatgt atctccaatt acatctttaa
    20581 taccatattt ttcaatcaat tgacgatgta tagtactttg ccatttacct tccatcatca
    20641 taggcacttt atgtaaacga tgcaaagcta aataacaaat aatactttga accggtgatt
    20701 tcatattaat agcaacaaca gggttaagtg aatatttctg caaagattca actgcgctag
    20761 tgatgcgggc atttagttga ctatatgtaa ttgtttcatc atctatatgt aatgctatgt
    20821 cattaggctt attttgagta tgtgtttcca actgttctaa aattacgtcc atcaatatcc
    20881 ctcactcaat gtaaacttta taatacatct tgttaacatt atataatgaa taagtatttt
    20941 tatcaggtaa acctgcctat tataaaaatc aaacttaagc cctagataaa tttgatactg
    21001 tagtaccatg ttttttatta gataagacga tactatatgt atatagagaa aagaagttat
    21061 cgcaatcgca ataactgaca caaataagtc acccaatcta tttaagtttg tgttctctat
    21121 aataatattg aaggttagag tctcaatatt aattgcgttg gacaaaatac tctaagacat
    21181 atttacatcc taacatttca atagcaagag aattttaagt attcaaagct aaacatatgc
    21241 tttaagtccc ctctttcccc cattgcgata atcattgcag tgggggttta tgtatataca
    21301 aaaactttat ttcggctaat tacattccct aatttaaaaa gcactttaaa gttagtagcg
    21361 tttactagct ttaaagtgct tttatacgat tgattattac ttatttactg aaatctctaa
    21421 tctttacgtt ctttcaccca ccataatgct tcttttgggt gttctacata ataagctcta
    21481 tcttgctcgt tatctacatt tggatatgaa cctttgagag attttcctgc tgtgctcaaa
    21541 tgtaagaatg tgatcactaa aaacccaata acactgattg ctgttaaata atacgcaggt
    21601 gctaatggat caccagtttt cgcgactaac catgttgcaa ctaatggcgt cgtaccacca
    21661 aatattgaaa cagagatatt aaaggttacc gataaagttc gatatcttat atgactgtaa
    21721 aacattgttg gtaaagaccc tggcattgtc gcttcgtatg ttgataagaa gaatcctaat
    21781 ccaaatatac cgatgactat cacaacaaat gattgtgaat gtaataacat aaatgcgatg
    21841 atactaaata atgttaaccc gccagtaccg attagaaata ctttcttttc acctatttta
    21901 tccgctaact taccaaacat taatgctaat ggaatcatta ttgccatgac acaagtaatt
    21961 aatacactag ttgtcgttgc atctagttta ataacttgtt ctaagtaagt tggtaagtat
    22021 gcggttacca tatagttcgt aacattgaag aatacaacag ctacaaaaca cacaaatata
    22081 tctttgtaat aaaatctgat gatttgtaaa aagttaatgt tgtctcgttc tggttgtgtt
    22141 gcaacatcat tttcgaaaac tggtgattct tcaagcttac gacgtaaata taagccgaat
    22201 aatcctagga ataaaccaag taagaatggg attctccaac caaatgatgc catttgttca
    22261 tcagttaaga agaaagtgag taaggcaatc ataattgacg cagctatata acctgataat
    22321 gtccctattt ctaatccact acctaatgag ttacgacgct tatctggaga tgattcggct
    22381 acatatgtca ttgcccccgc atactctcca cctgttgaaa atccttgtag tactcgtgca
    22441 agtaataata gtattggtgc ccaaagtcca atttgatcat aacttggtaa taatccaatg
    22501 gttaatgttg aaaatgccat taaaataatt gttgatgtta atacaacttt acgtccatat
    22561 ttgtcaccaa taataccaaa tacaacacca ccaattggtc ttaataaaaa cgcaatggct
    22621 aatgctgcga aagtcaacat ttgtcgaatg tctgcactct ctactggaga gaagaagttc
    22681 gctccaatgt acgcagttgt atatgcatag acaccaaaat cgaaccattc cattgcatta
    22741 ccgataccgg ttgcaacaac agtcttttta gctttctttg catccaccat gttaatattc
    22801 tctttattaa aatccatgaa ttcatacact ccctcttgta tgtttcataa ttatacggag
    22861 ttaattagtt ttgtcaaatt tataagaatt taaactttta tataaataca aaagtatgtt
    22921 tgttcaaaaa tttcacttta tcgaattaaa ttcaatgatt ttataccctc gtacatttga
    22981 attaaacaat aataattctt aaatcatcga acgattaact ttttggaatt ataactaaaa
    23041 atagcgatag catacaactt catataagtc atatgctatc gccattttcg acatcgtatt
    23101 taaagtaaga tattcaattg actgcaagta atatgtcatc agtcggagtt atttgtatta
    23161 atatcaaata attaattacg cagacttttc tatttaaaca tacacgatta ttaataaata
    23221 tttattttaa attcctaatc taaagcgaaa tatttctatg tataatctaa tatttataaa
    23281 tatggatttt aaccatggta ttctctgttt taacaaattt tataatacgg tattaatttc
    23341 tgatggatca ttgattataa aatctggatt tgaatgaagt aatgaccttg catcatgtgc
    23401 tccccatgtt actgctgcag atggtacacc agcatgttgc gccatctcaa catcaaatgt
    23461 tgaatcacca atataaaccg tttgttggct atttaaattg tagcgttgca caattgtgtg
    23521 tatgccttca ggatttggtt tatatgcact tacttgatcg gatccaacag cttcagtgat
    23581 taaatgattt aaccctatag ccgataaatt tctttctaat acatcactct tcttactaga
    23641 caccacgaaa agttttttcc cttggttata caaacttgta atggcttcag ttatacccgc
    23701 aaattcataa atatagtcct tttcaataga ttgatatgta tgtctaaatg tatcgattaa
    23761 ctttgctaat gttgcttcat ctaatggtcg gtctgctaat tttaaaaatg attcttcaat
    23821 aggtattccc atataatgcg atatgtcttg agctgatggt tctgttaaac cacatgcttt
    23881 aaatgcactt tgtgttgcta cttcaccaca ttttttcgtg tctgccaacg taccatcaaa
    23941 atcaaaaata taattttcaa acttcaatat taacacccct attcgtttat tattgatatt
    24001 atactataat ttattattta aataaaaaaa taatcgataa tatttctgcc taggcatcat
    24061 attatcgact atctttgttt tattctaaat tagcatgatt ttgttgcatc gtttgttctg
    24121 aaacattcat ttcagtcatc aatcccatta caacgctatc taaaaatagt tgagatgctt
    24181 gttcaaacaa acttcctaac ggttgtgcag aaccatgttc atcatatttt gtacctgcag
    24241 gcaaaacgat gttcgtccca gctagattgc ctattgcaga atctttattt gtagtaatta
    24301 atacgatgtc agcacctact gattttgctt tatctgctaa taatcttaaa tgttccgtgg
    24361 aacctgaacc agagataatt acaaatacat cattcgactt aatcgcaggt gtcgttgatt
    24421 ctccaacaac atgtgcctgt ttgccgagct gatttaagcg cattgcaaaa ctattcgcca
    24481 cgaatcctga acgtcctttg ccagctacaa atatatgttc agcatgtagt attttggacg
    24541 caaacgttga aaactcatcc gcttcaacat gtgacaaagt catctttaat tcatctagaa
    24601 ttaattgata gtcactaaat ttagccatat tacttacctt cgattgcagc gcgacattgt
    24661 tttgcagctt ctactggatc atctgcattt gcgattccgc caccaacaat aacaagatca
    24721 ggactttcag cgacaatttc tttaattgta tctggtttaa ttccacctgc tactgcaact
    24781 ttagaatttt taataacaga tttaacggtt cttaaacttt ctaatggtga ttgcccttct
    24841 gcttgtaaat cataaccagt gtgtactgca atataatcag cacccatttc atctagttct
    24901 tttgcacgtt tttctaaatc ttgaacagca atcatatcaa ctagtaattg tttattattt
    24961 ttatgagctt cttcaatagc tgctttaatt gatgcatctt ctgcaacacc tagtattgta
    25021 attacatccg cgccaaattt aattgcttgg ctaacttcat aatcagctgc atccataatt
    25081 ttcatgtctg ctaatacttt tacattacta atgttgtctg ccatatgttt aactgctggt
    25141 aaaccttcgt tgtaaatgat tggcgtaccg atttctacga tatctacata atcttttact
    25201 ttatttgcta actcagccgc gtcttctttg tttaataaat caattgctaa ttgtaattcc
    25261 actaaaaatc atccttccaa tgctttttat ctttctattg gaacctttcc cgaggcattt
    25321 cttaattaaa cattgcaatc aatccatttc atcgacatga attttcgcaa cgtttttcgg
    25381 gcaagcttcg tcgtctaaat atatctcaac attcgggtgt ttgtgaagta atgtggctgg
    25441 aacatcaaca gaaatttcct gatttaataa atgtgtaata gcagcacgtt tcttttcacc
    25501 aaatgcgagt aaaatgatac gtttagcttg aagaatatta gcaagtccca tcgaaatggc
    25561 ttgctttgga acatcatctt cgttttcaaa atatcgacta ttagccttaa tagtactttc
    25621 agtcaaatca acgatatgag taacgctatc aaacggcgta ccaggttcat taaatccaat
    25681 atgaccattt tcaccaatac ctaaaatttg aatatcacgt tgaccttgtt gttctaaaac
    25741 gtcattatat gttgacgctt ccgcattcat atcataggca tctccattcg gaatatgaat
    25801 gttctttcta ttaaaataag ggtattgttt gaaaagcatg tcatccatat aatagtgata
    25861 actttgcgga tgtgatgcgg ttaaacctac atattcgtct aaattaaacg tggatacatt
    25921 gtctacgttt aactgatttt tatttaataa cttaacaagt tgctcataca aatctgtcat
    25981 tgtgccgcca gttgctaaac caagtttaca gtgctgatta aatgccatct ctttatataa
    26041 ctcacatgca acatagaatg atgcttgttt ttttgaacct aagtttaata ctttcattgt
    26101 ttacatgcct ccacaaattt aatacatttt aaaaacttat gaattcattc atacaccatt
    26161 aatatagcat catttttaat gtttaacact tttcaaattc caacaatttt tttgattttt
    26221 aaaagtaaaa aactaatgct gtatgattgc gtaatcctga gtcattcagc ttcatgaaaa
    26281 ttaatttata aataattgag caatcgtata caattattga atagttttta gtaaaataga
    26341 aagataaaca cattgatagt gtaaggagtg aacgtgttgg aaccaattaa agaacaagaa
    26401 gttatagatt tacttgcttc ttttgaacat aaacctgtgt atctgcatgt tgaaacgaca
    26461 aatggggcct atgcgaacca tttcgatcaa cgtgttttta atgctggtac ttttttaaga
    26521 aacatacaag taacatatac acatgcacaa ctaaaaggtg gtaataaaga accttatcgc
    26581 attggtctta aactatcaaa tggtggttgg gtttatgtac aaggtctaac gcattttgaa
    26641 atcaatgagc atgatgaatt tttaattgca ggttttaatt acgaaggtca actcgctgca
    26701 gcacttcaaa ttagtgagcg accatttaat ttatagagga ggcatcaaca tgactgacga
    26761 aagacatgta ttagtaatat ttccacatcc tgatgatgaa accttctcat ctgcaggcac
    26821 attagcaagt tatatccaaa aaggcacacc tgtaacatat gcatgtttaa ccctaggaca
    26881 aatgggcaga aatttaggta atcccccatt tgcaacacgc gaatcattac caagtattcg
    26941 tgagcgtgaa ctagaagaag cttgtaaagt tattggtatt actgatttaa gaaaaatggg
    27001 tttacgtgac aaaactgtcg aatttgaacc ctatgaacat atagatggta tgattaaatc
    27061 tttaatcgat gatacaaatc catcattgat tatttcattt tatcctggat atgcagtaca
    27121 tcctgaccac gaagcaacag cagacgctgt aattcgaacg gttgaacgca tacctaaaga
    27181 agaacgccct cgtttaactt tagttgcgtt cagtaatgat gcaaccgaag cacttggaga
    27241 acctgatatc caaaatgata ttacagattt taaagaactt aaaattaagg catttgaagc
    27301 acatgcttct caaacaggtc catttttaaa acaattagca agtcctgaaa tcgatggtaa
    27361 acaacacagc tttttaacag tcgaaccata ttggacatat cactttaaat cttaacggag
    27421 gaaaaaaatg actgaatttg atttatcgac acgagaaggt cgttggaaac actttggttc
    27481 cgtagatcct attgaaggca cgaaacctac tactaagaac gaaatgacag atttacaaag
    27541 tacacataaa gatttcttat ttgaaataga agaagttggt attaaaaact tagtgtaccc
    27601 tgtacttgtt gaccagtatc aaacagctgg cacatttagt ttttcaacaa gtttaacaaa
    27661 agacgaaaaa ggtattaata tgagccgtat tattgaaagt gttgaaaagc actatgataa
    27721 tggtattgaa cttgaattta atacacttta tcaggtgtta tgcactttac aaacgaatat
    27781 gaaacaaaat gcggcaggcg ttgatgtttc tgggaaatgg ttctttgatc gctatagtcc
    27841 aaccacaaat attaaagcag ttggtaatgc cgatgtcact tatggattag caattgacgg
    27901 tgataaagtt acacgtaaag aacttactat tgaagcaact gtcacaacgc tatgcccttg
    27961 ttcaaaagaa ataagtgaat attcagcgca caaccaacgt ggtgttgtca ctgttaaaac
    28021 atatattaat aaagatcaag acatagttga tgactataaa aacaaaattt tagatgcaat
    28081 ggaagctaat gcaagttcaa ttctataccc tattttaaaa cgtcctgatg aaaaacgtgt
    28141 gacagagcgt gcatatgaaa atccacgctt tgtcgaagac ttaattcgtt taattgccgc
    28201 ggacttagtt gaatttgatt ggttagaagg tttcgatatc gaatgtcgta acgaagagtc
    28261 tatccatcaa catgatgctt tcgctaaatt aaagtatcgc aaataatatc atatgattaa
    28321 ttcaaacgcg taacagtagt tgcgcgtttt tattatacct cctagagttg tatatctctt
    28381 ttataaatct tctcatttga tgtgcctgag tcataaatca atatcctaag ctctacaatg
    28441 atatattggt agtatttgac tgaataacta ctaccattat aaatataaaa atttgttgtt
    28501 ttataaataa ac
//
LOCUS       DATTQF010000021        26632 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.021, whole genome shotgun sequence.
ACCESSION   DATTQF010000021 DATTQF010000000
VERSION     DATTQF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.021"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1012)
                     /locus_tag="VB241_000816"
     CDS             complement(<1..1012)
                     /locus_tag="VB241_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEP1244136.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLQGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVE"
     gene            complement(1042..1368)
                     /locus_tag="VB241_000817"
     CDS             complement(1042..1368)
                     /locus_tag="VB241_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEP1244137.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGVILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            complement(1417..1602)
                     /locus_tag="VB241_000818"
     CDS             complement(1417..1602)
                     /locus_tag="VB241_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244138.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            complement(1586..2818)
                     /locus_tag="VB241_000819"
     CDS             complement(1586..2818)
                     /locus_tag="VB241_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244139.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGIIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQLHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            complement(2811..3710)
                     /locus_tag="VB241_000820"
     CDS             complement(2811..3710)
                     /locus_tag="VB241_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244140.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDMSLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTHIRGIIHSENMKQGLQLTIENEDVSKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            complement(3943..4590)
                     /locus_tag="VB241_000821"
     CDS             complement(3943..4590)
                     /locus_tag="VB241_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEP1244141.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMMLWHLILMV
                     PAVCVLIISLFIFFISIAADNEALIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQSVTSETPKKAGTSETPKTPEETDNQL"
     gene            4989..5597
                     /locus_tag="VB241_000822"
     CDS             4989..5597
                     /locus_tag="VB241_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEP1244142.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEDG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            5933..7141
                     /gene="gltS"
                     /locus_tag="VB241_000823"
     CDS             5933..7141
                     /gene="gltS"
                     /locus_tag="VB241_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEP1244143.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(7189..8238)
                     /locus_tag="VB241_000824"
     CDS             complement(7189..8238)
                     /locus_tag="VB241_000824"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP1244144.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMTYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(8269..9216)
                     /gene="corA"
                     /locus_tag="VB241_000825"
     CDS             complement(8269..9216)
                     /gene="corA"
                     /locus_tag="VB241_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEP1244145.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFSFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            9313..9804
                     /locus_tag="VB241_000826"
     CDS             9313..9804
                     /locus_tag="VB241_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEP1244146.1"
                     /translation="MVIITQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            9801..10220
                     /locus_tag="VB241_000827"
     CDS             9801..10220
                     /locus_tag="VB241_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244147.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(10328..11248)
                     /locus_tag="VB241_000828"
     CDS             complement(10328..11248)
                     /locus_tag="VB241_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1244148.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            11456..12094
                     /locus_tag="VB241_000829"
     CDS             11456..12094
                     /locus_tag="VB241_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244149.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVNFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(12340..14277)
                     /locus_tag="VB241_000830"
     CDS             complement(12340..14277)
                     /locus_tag="VB241_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEP1244150.1"
                     /translation="MTTTFIISYIVLALIIVGVINLLLIRSRRKAKRQQKEQHFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNKDNQNQVSLNKQTEGSEQDQASFTDNN
                     ESDEEVYAAKNPNSEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTL
                     LNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFT
                     IGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMI
                     LAPAIGPTLSGYIVQNYHWNVMFYGMFIIGILAILVGFVWFKLYQYTTNPKADIPGII
                     FSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLK
                     FPTFTLTTVINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFA
                     GKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMV
                     TAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNP
                     VVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQ
                     SNKKAKATAQKIDADNSINHE"
     gene            complement(14290..14937)
                     /locus_tag="VB241_000831"
     CDS             complement(14290..14937)
                     /locus_tag="VB241_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEP1244151.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASVKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            15058..15612
                     /locus_tag="VB241_000832"
     CDS             15058..15612
                     /locus_tag="VB241_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1244152.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKRNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQKIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQNQ"
     gene            complement(15736..15933)
                     /locus_tag="VB241_000833"
                     /pseudo
     CDS             complement(15736..15933)
                     /locus_tag="VB241_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16367..17575)
                     /locus_tag="VB241_000834"
     CDS             complement(16367..17575)
                     /locus_tag="VB241_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEP1244153.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(17685..17812)
                     /locus_tag="VB241_000835"
                     /pseudo
     CDS             complement(17685..17812)
                     /locus_tag="VB241_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17843..19225)
                     /locus_tag="VB241_000836"
     CDS             complement(17843..19225)
                     /locus_tag="VB241_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEP1244154.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLVNENGKQVDKEYELEQGPQD
                     RLQLIKTSEK"
     gene            complement(19465..19920)
                     /locus_tag="VB241_000837"
     CDS             complement(19465..19920)
                     /locus_tag="VB241_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1244155.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            20115..21083
                     /locus_tag="VB241_000838"
     CDS             20115..21083
                     /locus_tag="VB241_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEP1244156.1"
                     /translation="MLLNLLLIFILIVICPLILNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRNRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPIPEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            complement(21313..21978)
                     /locus_tag="VB241_000839"
     CDS             complement(21313..21978)
                     /locus_tag="VB241_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244157.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRSSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSKRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(21978..23033)
                     /locus_tag="VB241_000840"
     CDS             complement(21978..23033)
                     /locus_tag="VB241_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244158.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQESYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYSHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVNEKDLTD
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            23169..23843
                     /gene="hssR"
                     /locus_tag="VB241_000841"
     CDS             23169..23843
                     /gene="hssR"
                     /locus_tag="VB241_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HEP1244159.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAFTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            23836..25209
                     /gene="hssS"
                     /locus_tag="VB241_000842"
     CDS             23836..25209
                     /gene="hssS"
                     /locus_tag="VB241_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HEP1244160.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDHKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTNDGSIAVFMRPDIGETFS
                     EFRTFLAVLLILLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESIDFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNAYVTTFTI
                     TLPNHSH"
     gene            25372..25815
                     /locus_tag="VB241_000843"
     CDS             25372..25815
                     /locus_tag="VB241_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1244161.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRFLKSIKERLSI"
     gene            25812..26267
                     /locus_tag="VB241_000844"
     CDS             25812..26267
                     /locus_tag="VB241_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEP1244162.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINYLIIFTIIFIVVYTLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(26444..>26632)
                     /locus_tag="VB241_000845"
     CDS             complement(26444..>26632)
                     /locus_tag="VB241_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEP1244163.1"
                     /translation="IEINFVPVLNTKMLTTKEKYFLLIFMCNAIIGVLQDWVKRGYKE
                     SPKEVAEIMNKIFENAFR"
BASE COUNT         8818 a         4711 c         3719 g         9384 t
ORIGIN      
        1 tttctacttt ttcttcgata cggaaacttg ttttcgaatt gaacacttca ccagctttta
       61 aaattgacgg tgctttttca ccatataaat taatatcatt tggtaaaaat tgtgtttcta
      121 atgtaaagcc agaatgtggt ttataaatat taaatggact atcccactca tcaggctgat
      181 taaaagtaaa gaatacaaca tgaggcatat ctgtatcgac ctctaacata aattcatgat
      241 tttcaacata cattttatgt tcaccaactg taaatgggtg atctagacca ccaaaacgtg
      301 tcatttgttg gcttaattgc gcatgttgtg atgtaaaaat atcactcaat tttatcttgt
      361 tatcttcaaa cacttcatgt aaatctagaa tatcacctgt aacaatattt cgctcatcta
      421 atacatacat atctaattga ttacttgaaa tgcgatgatt atcaacgaca ttattatctc
      481 gattcaaatt gaagtacaca tgattcgtag gactaaacaa tgtgtcttct gatgcaactg
      541 cttcgtattc aatcgaccat tggtgatccg catcataaat atgtgtaatc gtcacatcga
      601 tatcacctgg gaaatgatca tcagctgatt tcaacaccgt tttaaatatc actttaattt
      661 gagcaatttc atttctaatt tcataatcaa ataacttatt gtccaaacca tgacatccac
      721 catgtaaatg atgttcgccg ttgttttttt ctaactgata ttctttacct tgcaacttaa
      781 atttagcatt atcaattcta ccgctatatc ttcctataga agcaccaaat ttaaaaggat
      841 tactatgata aaattcatcc gcttcaacaa catttccaag aacaatatta ttatcatgat
      901 atttccaaga cactactctt gctccataat tcgtaaaaat aattttagtt tcatcattat
      961 caattttgat taaatctaca ccttgtcttt ggtgctcaac ttcaactatc atttttactt
     1021 ctcccttcta accacaagtg ttcaagctct gctgggcagc aacattacta aaacacctac
     1081 aatacaaatg attgcaccga taacatcata tttatctggc atttgtttat ctacgaccat
     1141 cgcaaaaatc aaactcatga tgataaatac gccaccatat gctgcatata ctcttccgaa
     1201 tgatggaaat gattgaaatg tcgcaatgac accatataac atgagtatca caccgcctat
     1261 tagcccaaca agtgaagact gtccttccct aagccacagc caaatcaggt atcccccacc
     1321 tatttcacat aagccagcta atataaatat aaaaatcgga tataacatga aatcactcca
     1381 tcacacattt gctaccaata atctatcggc tacatatcat ttgtttacat ttcttcttac
     1441 ttcacattcc cattttaaaa agttcgtttt cacattcata ttgtacactt ttttagacat
     1501 tattctatag ctaaatataa aaaaataaga gtaacacgct ttcatcatca ttttatatga
     1561 taaatgtgtg tcactctcat caattttatt ttttaaatac acgtttcatt gaattaaata
     1621 agccacgttc aaatgtaagt actgaatctt tatatgtttt aattgcaatc catatcaaga
     1681 cagctaccat tacaattgag attaatgaac ttaagatgac ttcatgtaat tgaagccctg
     1741 aagtttgagc gcgtacaact aattgaaatg gcgccaaaaa cggaatataa cttgtgatta
     1801 aagcaagtcg cccatcagga ttatttatcg tgaatatggc gatataaaat gcaatcatac
     1861 caagtaatgt cagtggcatc aaagattgat ttaaatcttc tattctagat gttaatgatc
     1921 cgagaatggc tgcaagtaat acatacgcca taattccaac aatactactt ataattccga
     1981 caataataat ttgccaagac aattgattca tttccacgtt aaaaccttgt agcaagtctt
     2041 ttaagtcaaa ggcaaaaatg catatgactg ccatcaatac aattaaaata atctgagtca
     2101 cggcaactgc tataatgcct aaaattttag caaaaatatg ttggattggt gaaatacttg
     2161 taataatcat ttcaatgaca cgcgatgttt tctcactagc aatttccata gctatttgag
     2221 atgcataatt taaaacaata aagaacatta gaaagataat gccataagct aaagcatagt
     2281 taaaaatctt ttgtccttct gatactttat cgacttcatc attagaaatc accttattat
     2341 caactttact ttgtgcttgt aatttttgta agtcttcttt gttgatattt aattccccgg
     2401 ctaccatatt tgtttgaata gctgtaagta gtgcttgtac tttttgtgaa tcttcatgac
     2461 ttactcgctt ctcactaatg attgtccctt gcaacgtgcg attttgattc accttgataa
     2521 tataagcttt atcaagttta tgttttttta cttctttttc agcatcttct atagaaactt
     2581 tagtaaactt agcatcacta tgaaatgtat tcgcctgttg cttgaaaacc ttatagattt
     2641 gttcattcgg tgctgctaca ccaattttat ctggaccatc atcaaacatg ttaataatct
     2701 tatcaatgtt agataggcca atcattaagg cagcaatgat aatcataaaa attacaaatg
     2761 atttagcttt aatttttttg atatatgtca aagtaaatgt cgcccaaaac ttatgcatcc
     2821 ttgccaccaa ccttctcaat gaatatatct tgtaatgatg gttctacaac ttggaatcgt
     2881 ttaacataac cttgatgtgc tacaacttga taaatatctt tggatacgtc ttcattctca
     2941 atcgtcaact gaagaccttg cttcatgttt tcactatgaa tgatgcctct aatgtgtgtt
     3001 aaatctggta gtgttgtttc tgactcaatg acaactttct tgttaccatt agatgcacgt
     3061 acatgattga tatcaccaga aacaacaagt tgacctttat ctaaaataca aacatcatca
     3121 cataattctt caacatgttc catacggtga gaactataaa cgattgtact gccccaatca
     3181 tttaagtctt taactgcttc ttttaataac tcaacattaa ctgggtctag accactgaaa
     3241 ggctcatcta atattagtaa ttctggttta tgtaacatac ttgctaacag ctgaattttt
     3301 tgttgattcc cttttgatag actatcaatt cgttttttac ggttttcagt aatatcaaaa
     3361 cgctcaagcc aatacgatat ttgctgttgt atttctgttt ttgacattcc ctttaaagtt
     3421 gccaaatatt tcaattcttc ttcaactgtc aatttcccat gtaaaccgcg ttcttccggt
     3481 aaataaccaa tacgattgta cattgtttta tctagttttt taccgttata cgtaatgtgt
     3541 ccttcagttg gttcacttaa gcctaaaatc atacgaaatg tcgttgtttt acctgcacca
     3601 tttcttccta gaaaacctaa cattttgcct gattctaact ctaatgacat atcatttact
     3661 gccgtcatct taccaaaacg tttcgtaaca tgctcaatta caagtcccat cttttgcctc
     3721 ctaaaacaat atgtatttat cttaatataa catttctatt ctctataaat acaatatttt
     3781 taaagtgaat ttatttttaa aatttctgaa attgaaatat ttaaatagta tcttctttgc
     3841 atgttaagta tcatgagcgt tagatatgct tttttccatg cctttattgc cttattggta
     3901 aaaaaaatat cccagcaaca atctgctgag atatctatat cattacaatt gattatctgt
     3961 ttcttccggt gttttcggtg tttcagatgt ccccgctttt ttcggtgttt cagatgtcac
     4021 tgattgattt ttaattgaag atgggtattt tgggctttcg ccatatttat ttgccggctt
     4081 actgtcaaag cataggaata ctatcataac aattgttagg cctaaataaa caaaataaag
     4141 aagtactaac aaaatattaa gacccatcgg cattaaggta aaatcactgt cataataact
     4201 atcgataatc tgtaatacta tataaaatat aatactgaat actgtcataa tcattggaaa
     4261 taacattgtt cttgatatat cgtgaaatct tcgaacgcac aacgctaaat ttggaataaa
     4321 cgttgccaaa ctatagacaa aagtatacac agatgtaagg ataatcatca atatactcat
     4381 aactattaat gcttcgttat ctgccgctat agaaataaag aatataaata ggcttattat
     4441 tagcacacac acagctggaa ccataagtat taaatgccac aacatcatat accaatattc
     4501 actacgtctt gatctcccct taaaattaac ataatttttc caaaataata cgaatgattt
     4561 cataaaacct acttgaggta attgttccat tgtaatctcc ctttcgttaa tcatatttat
     4621 atttttaatt attattaccg ttataattta caagattcat tatcaagcca cttcatttca
     4681 atcgtcattc actttggaaa atgaagttta tatttcatca ctagttaaat gaaaacccgc
     4741 tacaagtacg catctatatg gagactcatt tgaaagtcaa tgcttcgtta actatactaa
     4801 aaatatgtca cactgcaatg ttcatgttta aaagagtctc aatctctgca aataaaatat
     4861 tccataacaa agtatatact ttacattttt ataattctta acaatactat tttatcaaac
     4921 atttactaca ataaaaatat ctttttcatt tttatttaaa ttaatcatat aattgcgagg
     4981 agaatattat ggatttcgtt aataatgata caagacaaat tgctaaaaac ttattaggtg
     5041 tcaaagtgat ttatcaggat accactcaaa cgtatacagg ctacatcgtg gaaacggaag
     5101 catacttagg tttgaatgat cgtgcggctc atggctatgg tggtaaaata acacctaaag
     5161 ttacgtcatt atataaacgt ggtggtacaa tttatgcaca tgtcatgcat acgcatttac
     5221 tcattaattt tgtaacaaaa tctgaaggta tacctgaagg cgtacttatc cgcgcaattg
     5281 aaccagaaga cggtttatcc gctatgttcc gtaacagagg taagaaaggc tacgaggtaa
     5341 cgaatggtcc aggaaaatgg actaaggcat ttaacattcc acgggctatc gatggcgcta
     5401 cgttaaatga ctgtagattg tctattgata ctaagaatcg taaatatcct aaagatatta
     5461 ttgctagtcc acgaatcggt attccaaata aaggtgattg gacacataaa tctttacgtt
     5521 acacagtgaa aggtaatcca tttgtgtctc gcatgcgtaa atcagattgt atgtttcccg
     5581 aagatacttg gaaataaatg tcatctttca ttgattacta tcatgaaaat gaaatctatc
     5641 tccttataag tcaatcaatc agccgtcaac atgcggatgg ggtgattgtt tttctttgtg
     5701 tccatcatat tttttgattc atctcctctt tttgaacttg ttcttaattt taaaatataa
     5761 caatagaatt atttataatt attaaattta gatgtattaa tattattgat attattttca
     5821 aaaactagaa atattgattt attgcatgta taatgttaaa agtgcccttt tataacgctt
     5881 acatataaaa gcttatttag ggagagggat attcaacaag ggggatttga aaatgataga
     5941 acttaatgca attacaacat tatgtttagc ctgtatactt tacttacttg gtaaagctat
     6001 cgttaatcac gttaattttt taaaacgcat ttgtatacca gcaccagtga ttggtggctt
     6061 aatctttgct attttagttg cggctttgga ttcatttggc atggtcaaga ttaaattaga
     6121 tgcttcattc attcaagatt tcttcatgtt agcattcttt acgacaatcg gtcttggtgc
     6181 atcattgaaa ttatttaaat taggtggcaa agtcttgcta ttatacttta tgttttgtgc
     6241 tatcatttca gtcattcaaa acatagttgg tgtatcacta gcaaaagtat taaatattaa
     6301 accgttacta ggattaacag ctggttctat gtctatggaa ggtggtcacg gtaatgctgc
     6361 cgcttatggt aagacaatcc aagatttagg cattgactca gcattgacag cagcacttgc
     6421 ggctgcaact ttaggtcttg tatttggagg gcttatcggc ggaccagttg taaaattttt
     6481 aatcaaacgt tataacttaa agcctcaaca tagtgacgat acatttaaag attatagcca
     6541 agttgcatat aacgaacatt tacatagtaa atttaatgcc actgaagtat tcttcattca
     6601 atttacaatc gttgtattct gtatggcagt tggaagttat ttcagtcatt tatttacagc
     6661 tcaaacaggg attaatgttc caatttacgt tggctcatta tttgtagcgg ttattgtccg
     6721 aaatatctct gaaagtttta attttaatat tgtagattta aaaattacta atcaaattgg
     6781 cgatgtcgca ttaggtattt tcttatctct tgcgctaatg agcattcaat taatcgaaat
     6841 ttataaactt gctatacctc ttattattat cgttttagtt caagttgtcg ttatgatttt
     6901 atttgctgtt ttaattttat ttagaggttt aggaaaagat tatgatgctg cagtaatggt
     6961 aggtggtttt atcgggcatg ggcttggtgc aacgccaaat gccatggcaa atttagatgt
     7021 tattactaaa aaatatggaa actcacctaa agcatattta gttgtaccta ttgttggtgc
     7081 attcttaatc gatttaattg gtgttatagt cattatggga ttcattcaat ggtttagtta
     7141 aacaccaaac tcataaataa aagaggaggc cttcgcctcc tcttttattt atcctcgatg
     7201 tatattcaag ctacgttgtt ctatccatga caatatttcc ggactaaata cgatttgttt
     7261 ttgtgttaag tcgtcaatat ttttagcatc taacatcgtc attattgatt tcatgtgttc
     7321 aataaatgat tctacataag ctactgtatg tgcaatgcca ttattttcaa cttgatttaa
     7381 aaacggacgt gacataccag ttgcctttgc accaagtgct aaacttttaa ttgcatcgag
     7441 tggtgtacgt aaaccaccac tcgcgaaaac tgaaatttcg ctttgataag ccgttgtttc
     7501 aagtaatgac tcaactgtag actgtcccca tgatgataag taatccatat ctttatttgc
     7561 acgacgttca ttttcaatat ctacaaagtt agtaccacct ttgccactaa catcgacata
     7621 cttaacgcct atttgttgta agtcatgcat taattctttg ctcataccaa atccaacttc
     7681 ttttataatg actggaacag atactcgtga tacaatcgac gctatattat ctaaccaagt
     7741 cacaaattca cgattccctt caggcataac taattcttga ggagaattaa catggatttg
     7801 taacgcttgt gcctcaagta attcaactgc ttccaaagcc ttttctactg gtacgtccgc
     7861 accaacattg ctaaaaatca tgccttcagg attcattttt cgcgcaatcg taaatgtctc
     7921 agccatgcgt ggatttctca atgctgcatg tgttgatcca actgccatcg ctaagccagt
     7981 ttctcttgca actacagcta gcttttcatt gatgtttttc gtccactcgc taccacccgt
     8041 cattgcatta atataaaccg gatatgtcat cgttaagtca ggtgtctgtg atgtcaaatc
     8101 gatatcattt acattaattg atgggataga atgatgcaca aaacgcatct tatcaaaatc
     8161 tgaatgcatt gcgtcagatt gggccattgc tatttcaaca tgttcatttt ttctctgttc
     8221 tctttgaaaa tcactcatga ttaaacctac cttttcgtca tttctaattt aaaaccattt
     8281 tttacgtctg aaaaagatga ttaatacgac agctataaca aacattacag ctaagcaaat
     8341 ataatatcca taatgtaaat gtaatgccgg catatttaca aagttcatac cataaatccc
     8401 agctatgaat gttaacggtg aaaatataac tgatactaat gttaatactt gcataatact
     8461 attcattcta aatgacgtgt atgactcaaa attttctcgt atttcatttg tcatttcttg
     8521 tgcagtacga atgatattac gttgcttaat caagtggtca tcgatatgtt gaatgtatag
     8581 cgaatgttta ttatctataa tcaaatcacc attttgtttc attgtatcaa ttagctcctg
     8641 cataggaaac agtacacgtt ttactttaat caaatccgaa cgtaacttaa agacactatc
     8701 catgaccatt ttattaaagc gatcatctac atggcggtct tcaaaatgat aaacactatc
     8761 ttcaagtgca tatacaaagt tgaaatattt atcaaccatc atatctaaaa ttaatatgac
     8821 gacatctgca caatctaatt ctgcatctaa tgtattcata tacttataga ctactttatt
     8881 taatgattcc aacgtttgat gatgatatgt tactaataca ttgtcttgta taaaaatatt
     8941 tagtgctatt ggtgaatagt ttgaccccat aatactatgg aatactaagt attgataatc
     9001 tttataagat ttatatttag ctcgtggcat accgttaatt gcatcatcca cttctaaatc
     9061 attaaaacta aaatgtgctt taaaccattc attttcttgt tcattcggtt catcaaaatc
     9121 ataccaaaca atagtcgcat cttttggtat ctctttgata tcatcaacta ctttaaacgg
     9181 ttcgtatgta gtttgatacc gtatcgttaa agccatcgat actcccccta aataacgaat
     9241 tctctattat tttatcatga attaaataac gtgtatgtct taatttattt tagtatgata
     9301 gtcactaagg agatggttat tatcacacaa ctttttacac atactcaaac cgtaacatct
     9361 gaattcatcg accataacaa tcatatgcat gatgcaaatt ataatatcat ttttagtgac
     9421 gtcgtgaatc gttttaatta cagccacggt ctttctttaa aagaacgaga aaatttagca
     9481 tatacgctat ttacactaga agaacatacg acatacctct cagaattgtc tcttggcgat
     9541 gtatttactg ttactttata tatttatgat tacgattata agcggttgca tttattttta
     9601 acattaacta aagaagatgg gacactagca tcaacaaatg aagtaatgat gatgggaatt
     9661 aatcagcaca cacgtcgttc tgatgctttt cctgaatcat tttcaacaca aatagcacac
     9721 tattataaaa atcaaccaac tatcacttgg cctgaacaat taggacataa aatagcaatt
     9781 ccacacaaag gagcattaaa atgacagatg cattacaaca aaagattcat atcgaactac
     9841 tagatttatt agatgatgtt aagtttgaat taacagaatt aaatgcacaa aaagggttat
     9901 acattaacgg accagcaaat cagttactta agcgtggcgt gcatatggct tatgttcaag
     9961 gacaaaagca agctatcgat aatattatga ctattgtgga acaacagctt gaagatcaac
    10021 atttcctaga acattatgat aaatttcaaa atgaggttgc tcatcgcaat tatgataaaa
    10081 cagctaactt tgcagaactt agtgatattc cacgtcaatt cgataatttt ttagatcaat
    10141 tttatcaaat taaagggcaa tactttatca tcacacatat caatacactt attggtgatt
    10201 ttcactcaga agctcattaa caattagtct atataatcct tgctatattt tcaaaaacaa
    10261 aacccagttg cgttttcatg ttaaatatct tctaccatga aatcgtaact gggtcattta
    10321 tatgttatta gttgttttgt gttacatcct catctatcga tttggcaatt tgtttaatag
    10381 ctttatgtga ttgtctaatt ggataaattg gaaaatcatg taccatctta ggataatcat
    10441 aaaactcaat gtattgatga tgttgcaaca tcatttgttc gaacagcttc atatcaggat
    10501 gtgtcatttc acgtccacca ccaaacatat aaactggtgg taatccttct attgtgccat
    10561 taattggtga tatgcgctta tctgttaatg gtaggccatt cgcccatttt ttcataatct
    10621 cattgacacc aaactgactt agaaccgcat cttgttcgat taaagcatcc gaaatatctt
    10681 tattagatag tgttgcatct aaaattggtg agattaaata caatttattc ggtaatggct
    10741 gttgattgtc taaaagagat tgtacaaagg ataatgccag tgcaccacct gaaccatcac
    10801 ccatgactac gacattttga tggcctactt cagatactaa ttgatcataa acacgttgta
    10861 tcgcttggaa agtgtcgtca atatgaaact ctggtgtctt tggatagata ggcagtacaa
    10921 cctcatataa tgtacttaaa gtgattttat ccaacaatct ccaatggaac ggtgatggtt
    10981 gtagtgcatt gaatccaccg tgaatatata aaattttctt atcaatttga tgtctgaaat
    11041 taaagcgaaa gacttgcata tcatccaatg ataatttttc taaatttgct ttaacattta
    11101 atgttgaagg ctgcttatgt ttttttctat tttcaatttc tcttttataa aaaaatcttt
    11161 caacatcttg atcattttta aacataatcg agcgattgtg aagcaaatat ttattgacaa
    11221 cgctattcat aacacggttt ctaatcaatg tcttaaccta cctttatata ttttatgtat
    11281 ccaatgattg tctatcccct acattctttg ccaaaaaaag tatataatgt agaagatatt
    11341 ttctttttca ctttcaaatt taagactaca attgaacagt gatttttcat cattataaca
    11401 gacaactaga catattgata agtaaagaaa agaactttat acggaggtgc cttgtatgac
    11461 aaatccaaat caacgattag aaccatttga tgagacattt caacaaccga atattcatcg
    11521 tggtaagcga tatggtaaga aaaaacgttc attggtaagc atgattattc aaatcattgt
    11581 tgttatatta accgcaatcg ctggatacag catgtggaaa caacccattt ttaatattgt
    11641 cttcaccaaa gaatctgtaa attttgatgg cattaaaaat tttaacgaga caattggaaa
    11701 aatgcatcaa ttaaatatta atttgggtaa tatcaatgat ttacaacaag gaatcgatcg
    11761 cttaatttta gtctttaacg gtttctttat tttatgtatc gttagcctag tcataacaat
    11821 actgacaatt gtatttaatc gcacagttct aaaagtagta aatattttag tgttaacaat
    11881 tatgttagtc attacattgt atttcagtta tattattaaa gcactcgcac aagaaattgc
    11941 agatgcttta aaaaaatatt atttaaatgt accaccagat caagtgatta cagaagctga
    12001 cgctattcat aacggcatca ttttactggg ctgtagcatt ggtttacttg ttataagctt
    12061 attcttccgt aatcgcatgc ctcgattaaa ataatgttat atagtaggga ttgggacaga
    12121 aatgttattt tcacaaaatt catttcgttg acccacttcc tgctttttct ttattaatga
    12181 cctactacat caatgccaaa ttttaatata atttctctaa tattctacaa atcaacaaat
    12241 aaaaaagtat cgataaccat atattggcac cgatacttta aaaatcatgt ccccacaaca
    12301 taatttttga ttagtcacac ttcaattaac ttattttatt tattcatgat tgatactatt
    12361 atctgcatct atcttttgag ccgtagcttt tgcttttttg ttactttgta aaaataagca
    12421 taaaattaaa gcgatgatgc taaatatcgt tgcaactata aatgcatcat taatcccttc
    12481 aactgttgct agtttattta caaattgtaa tagcactttc attgcgcctt cttgaccacc
    12541 atattgcgat gctaattcac gcatatgatc ttgtacaaca ggattcgttt tatctaactc
    12601 ttccccaaaa gctgataaat gttgtgtagt ttgcgttgtc attacagtaa ccaagattgc
    12661 tgttcctata gaaccagcta gttgacgcat cgtatttaag aaggcattac catgggatgc
    12721 cagtcgtccc ggcaacgcat taatagctgc tgttaccatt ggcatcataa taaatgccat
    12781 accaaatgaa cgtagtacat agatgcccat gattgtcata tatggcgtat ccatattcaa
    12841 tttagttaat tcccatgttg cataagtcat gacagcaata ccaaaaatgg ctaaaggttt
    12901 taaaccaatc gtgtctaaca acttaccagc aaatggtcct agtagaccca taattagaga
    12961 accaggtaat aataacaatc cagaatctaa tgctgagaaa ccacgtaaat tttgtaagta
    13021 aattggtaat aaaatcatac caccatacaa gctcaacatt acaaccatat taattactgt
    13081 tgttagtgta aatgttggaa atttcaatac ttctaaattc aacattggtg ctttcattct
    13141 taattctctt attacaaata gaataataaa gataatacca atcgcaaaca ttgtttctat
    13201 ctcaacagaa ccccagcctt tgttccccgc ttctgaaaag ccgtataaca aagcaccaaa
    13261 accaatcgta ctaaaaatga tgcctgggat atcagcttta gggtttgttg tatattgata
    13321 taacttaaac catacaaaac caactaagat tgcaagaata ccgataatga acataccgta
    13381 aaacatcaca ttccagtggt aattttgtac aatataacct gataatgttg gaccgattgc
    13441 aggtgctaaa atcattgcga tacccattgt acccatggca gcaccacgtt tttcaggtgg
    13501 ataaattgta ataataacaa ttgaacctaa tggcattagt acacctgcac caatggcttg
    13561 taatacacgt ccaaccatca tgattgggaa attcattgaa atcgcacata ttaatgaacc
    13621 aattgtaaag agtactaacg caactaaaaa taattttcga tatgaatatt tattaaatag
    13681 atacgccgta attggtatta aaataccgtt tactaacatg aatcccgtca tcaaccattg
    13741 ccctgttgac gcagaaatat taaattccgt attaattttt ggcaaagcaa catttaataa
    13801 tgtttggttt aaaatcgcaa taaacatacc gaataataat gctgctaata ttttaccgcg
    13861 tgaaacacct tcaccaaaaa taaagttttt atgttctttt ttaattttct catttacttt
    13921 atattcttct gagttaggat ttttagcagc atatacttct tcatcagatt cattattatc
    13981 agtgaatgat gcttgatctt gttcagaccc ctctgtctgt ttgtttagac taacttgatt
    14041 ctgattgtct ttattgtcaa tacgtgcctc ttcacgcgcc gtatgttgtg acgattgatc
    14101 agttgttctg tctaaatcac ttgctttaaa tttagattga tttgattgac gtgtcgcaaa
    14161 atgttgttcc ttttgttggc gttttgcttt tcttcttgat cttattaata ataaattgat
    14221 aacaccaaca ataatgagcg ctaaaacaat gtagctaata atgaaggtcg tagtcattta
    14281 atgaccccct taatttttat ggatttttac ttcagcgttc attccaggaa caacttgttt
    14341 agacggttct gaatctaacg taattttaac aggtataact tgagatactt tagtatagtt
    14401 accatcacta tttgatgatg gcattaatga aaaactagca gcagtagctt ttccaatact
    14461 atcaacttta cctttaacag aagctttttg accgtcaata gtcacatcaa catctttacc
    14521 aacttcaaca tctttaatat ctttttcgtc aatatttgca gttacatata aatcatctaa
    14581 attgtaagca taagcaattg gattgcctgc ttgtaccata gaaccttcca tgccatctaa
    14641 tttagcaatt gtaccttttt gtggcatttt aagttccata tctttagttt cgccatcttg
    14701 accttgaaca gtaactgttg ctactttatc acctttatca agtttatcac cttgcttaac
    14761 atttagtgat ttgatttgcc cagaagctgg acttgctatt ttaatttgat cgccatttac
    14821 tttagcatta tcagttgtta tataacttgt tgttttattc cagaaataaa aaccagcaat
    14881 cccaattgct agcagtacaa caaccgtaat gacattaatt aatatcatct ttttcatgaa
    14941 acatttcctc ctatttgctt ttaaaattca ctttatttat tatagagaca tgataactaa
    15001 aaaaacacca acaattttta taagagtgtt ggttaaaatt cacaaaggtg taacaatatg
    15061 aaacgacaag ccaaaataga aattcaaaac gcacttgttg atttgatggc tgaatatcct
    15121 tttcaagaaa tatccactaa aatgatttgc gcgtattgta atattaatcg ttctactttt
    15181 tatgattatt acaaagataa atttgattta ttggatacca tcaactcaaa gcataaagaa
    15241 aaattccaat ttcttctaag cgcgctacat cataatttcg aaaatattaa acaggataag
    15301 cttaaacttt ataaattctt cattattatt gctaagtata tcaagcgcaa tgagcagttt
    15361 ttcaaagaca ttttagttac ctatccaatg aaaacattat tcatagacta tataaattta
    15421 gcgagagatt attatcaaaa aattatgaat gactatagta ctacagtaac caataaacag
    15481 ctttatgtta catatatcat aggcggtcaa gctggggtat ttattaattg gctttcgaat
    15541 ggttgtaatg aatcacctga agaagttgct gacattttat tggctaatac gattaaactt
    15601 caaaaccaat aatacggttt cattctaata aagaaatgct ataaaacaac tagataaagt
    15661 taaaccaaat ggacagataa tagtacatat gtaatggtgc tatcaatata aaaatgcgct
    15721 tgggacatta agctcatatg catataagat gatttttaac atcatttttg gatgatagga
    15781 tgttgcgcca cgatgatgtc tgaattcatc taattcgcta tcaggtatcg tttcaacaat
    15841 ttcattaaca tatcgcgaaa tatcattttg aggaattcta acagaagttt ctattggtag
    15901 tgtaagttgg gtcatattat gatttttata cataaggcac ctcgttaatt tagtttagtg
    15961 gtatttatta aattatacga agggacccaa cacagaaaat tcattttatt aaattttaca
    16021 tttatgtgca agttgggcaa agtgtcttat ttttttaaag tatttaaaag taaaattaca
    16081 tgttaatacg tagtattaat ggcgagactc ctgagggagc agtgccagtc gaagaccgag
    16141 gctgagacgg caccctagga aagcgaagcc attcaatacg aagtattgta taaatagaga
    16201 acagcagtaa gatattttct aattgaaaat tatcttactg ctgttttttt agggatttat
    16261 gtcccagcct catttttatt tttgatgcta aattcatagg tctatgaata gctcaagttt
    16321 tgtttttaaa tttttcaatt cattataata gaagaaacta tgtttatcat cgatttgttt
    16381 taaatacctt catattatat atctgtaaaa taattaatat aactgctgta gctataataa
    16441 taattatgta aggtatagga ttatcttcac cttttacccc cactaatggc gatgcaatac
    16501 caccaaataa aaactgcact aaacctaata aacttgatga actccctcta ccactactac
    16561 tttcatccat agctattgta aaaccaagtg tcgcaacacc tgtcactggt gctattaaaa
    16621 tgataaatcc tatcgctaaa atccaaaagt tccaatgatt aagcaataca atcgtcacta
    16681 aaattacacc gataatctga atcatcgtca tgcctctcat caatttttgt gagtctataa
    16741 aatcaacaag gtacccagta agttgacttg atataatgag cgtaatacca attcctgcaa
    16801 acatccaact aaattgtatt gcagtcatgc cataaatttt ttgaataata aacggagacg
    16861 cagaaatata agtaaataat attacaaatg tcataccttg aatgagcatt ggtaatacaa
    16921 aacgtggcgt cttcaataat attttgaagt ttttaaacat tgtctttaat ccactacttg
    16981 actcacgatt tgtcactgtt aatgattcag gtacttttaa taaagaacct atgaccatga
    17041 cgaagccaaa gatagtcaga atgacaaaga ccatacgcca gacagaataa tttaaaatta
    17101 tgccccctat tgttggcgca acaactggtg caataccatt aacaagcatc aataatgcca
    17161 taaacttagt tagttcatta ccactataca tatcacttgc tatagctctt gaaataactg
    17221 ctgctgctcc acctgtcact ccttgaagaa atcttaatgc aaccatcagc caaatattat
    17281 gtacaaaaac aatacctaaa cttgctaatg taaaaataat catagctata ataagcggct
    17341 ttctgcgccc agttgaatct gaaattggac cagcaaacaa attaccgaac gctaaaccaa
    17401 tcataaacat cgataatgtt aattgtgcgt tagacgtcgt tgtttgaaaa tcatgtctaa
    17461 tatctggtaa tccaggcaaa aacatatcta ttgataatgc tccaatggca gtcaaagatc
    17521 ctaaaataat aataaatatc ggtgaacgct tgttaaaatg cgttaaatca ttcatatgac
    17581 tatctccttt atattatgat tagtttataa ctatcacgaa cagaaactaa ttttaaatat
    17641 gctcttttat atattagcat tactcacaag tttcatgaac atcattacaa cttataaaag
    17701 gtataaaatg atataaacct cagcactcca taattttttc acaaaaaaca atcccttcaa
    17761 agtaattcac actaattgta aatactttga aaggatgttg tttgtattcc atgacacatg
    17821 catcttattt aagttttata ctctattttt ctgatgtctt gattaattgc aagcggtctt
    17881 gcggaccttg ttcaagctca tattctttat ctacttgctt accgttttca tttactaatc
    17941 ttacggttgc agtaatctta tcaccatgtt tttcggcatc tataatttgt ggtgaactaa
    18001 tcatcattaa acttccttta gatacacgct tctttacatc atcataaaaa gatgatcctt
    18061 tttttatata tgacgataca aaatcaaaat ccgactgatt aaacgcagca ttattcgcta
    18121 aagaatatcc agcaaagaat tctatcaatt tgttcttcaa gctgttttct tctttttctt
    18181 tcttttcaac atagtcttcg atatcttcac tgtcaaaatt caaagttatc tctgtattgt
    18241 attttaaatc gctggcttta atcgtcttcg tctgtgttgt aaatgtttta cctttcgctt
    18301 tacctgaagc tgaaatggta atatctttat tttgtggata tggaccatac gttttggaac
    18361 ttgaaaatgc catttcacgg tcatttatag ttactttttt agaactatca tttaattttg
    18421 tatcgccttt taaagttaca gttatatttg cttcttcaaa atcttcagta acatctaccg
    18481 tttcagaatt actttgtcta aaatcaaatt ttaaatggcc tgcaaaatca ccgttctcag
    18541 ttgatttcat agctggaatt ctatatgtcc ccggtataaa attacctatt ggcgtcactt
    18601 tatttgcttc agctataacc atctttttct taccaccaga tttaaactca tattttgttt
    18661 tttctttcgg tttaacaatt ggttgcttgg taggtgcagt aaagctcata ttatcgaaaa
    18721 agatataacg cgtgccattt ttacttacac gtaatatatt ttgaccagat ctggtttgaa
    18781 tataagaagc tacactggtc ttactcttat tcaatttatg taccgtattt ttaaggtcgc
    18841 tgacaaattg cttaagccca acttcatctt tgatatagtt aatgtatact ttcgcttctt
    18901 cagaatctac tttattatct ttagtactta ataatgttgc tactttttgt ttatcattat
    18961 tctcaatcgc attcactaat attttagttt gcgcttcagg tgaattgaag tttcttagta
    19021 aaaagaacaa tataataatt aagattaaaa tgaaaaaacc aattgcccaa ggcaccattt
    19081 ttctcatctt gatgttatta gtttgtatat cttcatcggt tgactttcta tataatgctt
    19141 gacgactatc aaatttatgt ccacattgtg tacatatctg tacatcatct tgtgcttgtt
    19201 gtccgcactt cgggcaagat ttcatacttt cacctcaatt ctgttcctct tcaattgtta
    19261 tatgtatatc ataaaaatta ttttattatt ttaccattat tattttacaa atattatccc
    19321 aagaatacaa ttcaagaaat gattaacttt actttagaat tgtaccttat tacattattt
    19381 atgaaagtac tctctcaatt ataactaaaa agaaggtgtt caatacatac atatttagtc
    19441 ttatatttga acaccttcaa aaacctattt atgattgcta tatgttttaa tgcgatgatt
    19501 gatgacttct aatacttgcc ttacattttc aatatcttca gaatttaaat cattagtaat
    19561 atcttgcgca atatctgtca taatcgcatt acgttctttc aaatatgctc tacctttgtc
    19621 agttaaggtt atgaatttca aacgttgatc aatatttaaa tttggtttat ctaacttaac
    19681 taatttagca tcgattaact ttttaattcg tcggcttact gcggccttat ttacaccttg
    19741 tctttgcgtg atttcactaa ttgtcaaagg ttctttattt aacataccta atacgttaga
    19801 ctgctctaat gaaatttcat attcattttg taaatctttc aacatttttg cagttaatgc
    19861 gttaacgtta tttataaact gctctaaaaa ttgaatatgg tcttgtaaat gttttaccat
    19921 aattatttct ccctaattac tatttttata aagtatcctc agcaatcatt gtatcgcaca
    19981 tctaaaaaac acaaaaatat tgacccatat aaaatataac aaattaagac ttacatgttg
    20041 ctacgttctc cttgctttaa taaattaaat catctacatt tatacttaat atttattata
    20101 agcgaggtgt cagtatgtta ttgaatttat tattaatatt tatacttatc gttatttgtc
    20161 cgttaattct caactatact ctgacgttaa atttaacatg ctacatctta gttgtcatat
    20221 cgatattttt gtcactcatc tatagcatca tgacctatac tttcccttgg tctatgtttg
    20281 ttacatttat aataatcagt attatcatgc tattcaaaca tagatattta ttttctcata
    20341 cttctagtcg cttatttatt aaaaaattat tatctttttc atataaattt gtactctaca
    20401 catcagcact tctatttata agtacgattc agtcacctat tgtgaaaggt ttgtctatgt
    20461 ggttagctat attatgtctt tccctgttac taacctttat ttgttatctc gtttggacag
    20521 tatcatatag aaatagatca tacaacaaag aagttgatat cataattgtg ttaggtgctg
    20581 gtatttttac cgaatttgtc acaccaatgc ttgcagcgcg tttagaccgt gcacttgata
    20641 tatatcaaca acaagcatca gcaacaaaga tcattgtatc tggcggacaa ggaccagacg
    20701 aaccaattcc tgaagcttta gctatgcaac gttatttgat tgcacatggt gttgataaaa
    20761 cagatatact aatggaatct cactctataa atacatacac aaacttttta tattctaaac
    20821 aaatcatcaa caatttgtat catgaagcag ttaaaattgt ttgtgtaaca agtcagtttc
    20881 atgtattacg cgccttacga ctagcccaaa aattaggttt atctatcgat ggtgtcggaa
    20941 gccatacacc atatcacttt ttcttacatg tgctcattaa agattatcta ggcgtaatgt
    21001 atcagtatag attgttatta acgttatatt gttcaagtag ttggtttata tgtctatacg
    21061 ctgcttttat ttataatagt taacccacaa ataaactatt attataggtt gtttctaata
    21121 ttgcgtcaaa ctatctttaa atattcgtta atccttgatt agacaattgt tatattgaaa
    21181 ataaataaac taaagcacgt catcatttta tataatagtg acgtgctttt tgtaaaccca
    21241 aatcgtattc tgttatctaa cttttatact actatgtctt aaattattaa catttagcgt
    21301 ctttacaagc cactaatcag ttattttgcc atcttctaat tcaatcactc gatctgcata
    21361 ttcaaataat cttcgatcgt gtgtaatcat aataccaatc atttgttgtt ctttaatttg
    21421 ttgacgtatc atctcaacaa cttttgttgc tctatcggca tctaaacttg ctgtaggctc
    21481 atctgctaaa atgattttcg gattattcat aaatgctctc ataatcgcaa cacgttgctt
    21541 ttcaccgcca gataactgat gcggatatac attcaagcga tcttctaaac caatattttt
    21601 caaaagttgt attgctcttt ttgaactttg ttgtttggtc attcccgctt cttggcctac
    21661 gagtgtcaat tgctctatca cttttaaata aggaactaaa tgtgaagatt gaaaaataaa
    21721 accaatatct tccaatcgta aatcactaga acgatgctgt ttatcaaaca atggtgcatc
    21781 attgtaaagc actgtaccac tcgtttgact taacaatccg cctaatatcg ttagcaatgt
    21841 tgttttccca gaaccagagg caccatttaa aatgacaaat tccccttgtt ccacttcaaa
    21901 attaatacct tttaaaactt ttgtttcaga caaaccttct ccgaaatttt tgacgatatc
    21961 tttaacaact aatgccatta ttctgcacct ccaattgctt cgataggatc caccttaaat
    22021 aatttgataa atgatagtga ggcacctaaa atcgctacta atataaatat ccccaccatc
    22081 aataaaatat tttgcgttgt taaataaaaa ggcatcgtta caggcatcat aaatgatagt
    22141 cctactatga tgatcacagc aatacctacc ccaattattg ttagtgttaa aatttgtaaa
    22201 actaacgcac tcaataaatg tcttgtctta ataccaattg ctttcaaaat gccaatttgt
    22261 gatatttttt gaatcgtcat aacatagaaa aatgcactta gaacaattgc tgtaatagca
    22321 aacaaactca caatcatcat atttaacggt gcttgctctg cttgataact cgcaatatta
    22381 tctgttaaat ctttttcatt cacaacttga acgtcattta ttttattaag ctcatcacgt
    22441 tgttgttgcg ttaaattttt cactggataa aatgccgtat taaccttatt caatgcatta
    22501 aatccgttat cgttcaatag cacaatggaa ctatgcgaat acattgtgtc gtttaaaata
    22561 ccagaaactc tataactagt tgaattattt ttaaaatgta atctatctcc gactctaatg
    22621 ttgtctgcag ctaacttatc attaatagcg acctcatgag catttttagg ataatcacct
    22681 tttttaagtt gtatgcgttg ttgtttaacg tcattcattg taatcacatc ttgttcttta
    22741 ttagataatt tcaatgtttg aatattcatt ttatatgatt cttgatgaat aaccttttta
    22801 atttgattct gttgcgtatc actaagttgc gatttctcaa tttgcggctc tttcattttt
    22861 tgaacaacat attcatcatt atcaaagtgt tcaaataacg aaatattctc cctaccaagc
    22921 ccttgagcta aaccactaat aaataacacc ataatactta ataaaagaat gattaatgtg
    22981 attaaaatat aacgaaattt gtaaaacata atctctttta tcgctaattt catatcgatt
    23041 cacttctccc tatttcttct ttagcgtcag tataaggttg aaatatgaac tcaatatgaa
    23101 caattgtttt tttacgcaaa aatattttac atgtataata aatgaaaaag ataagggagt
    23161 ttatagttat ggtgcaatgt cttgttgtcg atgacgatcc tcgaatccta aattatatag
    23221 ctagccattt acaaacagag cacattgatg cattcacaca accaagtggt gaagctgcat
    23281 taaaactttt agaaaaacag cgtgtcgata ttgcagtggt agatattatg atggatggta
    23341 tggacggctt tcaattatgt aatacattaa aaaatgatta tgatatacca gttattatgt
    23401 taacagcacg ggatgcactt agtgacaaag agcgtgcgtt tataagtggt actgacgatt
    23461 atgtaaccaa gccctttgag gttaaggaac ttatttttag aattcgtgcg gtattacgtc
    23521 gatataatat caattcaaat tcagaaatga ccattggcaa cttaacgcta aaccaatcct
    23581 atttggaact ccaagtatct aataaaacga tgacgttacc gaacaaggaa tttcaattat
    23641 tatttatgct tgcagcacgt cctaagcaaa tctttactcg cgaacagata atagaaaaaa
    23701 tttggggcta tgattatgaa ggagatgaac gaacagttga cgttcatatt aagcgactac
    23761 gccaaagatt aaaaaaatta aatgccacac ttacaattga aacagtaaga ggacaaggct
    23821 ataaggtgga gaatcatgtt taaaacactc tatgctagaa ttgcgattta ttccattacg
    23881 gttattttat ttagtgcatt aataagtttt gtattaacaa atgtttacta tcattataat
    23941 ttaaaagcat ctaatgacgc gaaaatcatg aagacgctta aagaagcaag acaatatgaa
    24001 caatcagcta aaccaacgca cattcaacaa tatttcaaac atttagggca aatgaattac
    24061 caaataatga ccattgatca caaaggtcat aagacatttt atggggaacc ttttagagaa
    24121 gatactttat cacaaaatgc tattaacaac gtgttaaata ataaagatta ccatggtatt
    24181 aaagataaac cattcgcatt attcgtaact ggatttttcg acaatgtaac agataatact
    24241 gttggtatta actttaagac aaacgacggt tcaatagcag tatttatgcg tccagatatt
    24301 ggagaaacat ttagcgagtt tagaactttc ttagccgtct tgttaatatt gttattattt
    24361 atctcaattt cattagttat cgcatcaacc tattcaatca ttcgtccagt aaaaaagtta
    24421 aagctagcga ccgaacgctt aattgatggt gattttgaaa cacctatcaa acaaacacgc
    24481 aaagatgaaa ttggaacatt acagtatcac tttaataaga tgagagagtc attgggacaa
    24541 gttgatcaaa tgaggcagca ttttgtacaa aatgtatctc atgaaatcaa aacaccatta
    24601 acacatattc atcatttatt aagcgagtta caacaaactt ctgataaaac attacgacaa
    24661 caatatataa atgatattta tactattaca acgcagctga gcggattaac aactgaattg
    24721 ttgctcttat ccgaattaga taatcaccaa catttattat tcgatgataa gattcaagtc
    24781 gatcaactca ttaaagatat cattcgacat gaacaatttg ctgctgatga aaagtcgtta
    24841 atcattttag ctgacctaga atcaatcgat ttcctaggaa atcaacgttt gttacatcaa
    24901 gcattgagta atttattaat aaatgctatt aagtatacag atgttggtgg tgccattgac
    24961 atcgctttgc aacatagtca caacaacatc attttcacaa taagtaatga tggttcacca
    25021 attagtccac aagctgaagc acgtttattt gaacgtttct ataaagtgag caaacatgat
    25081 aatagtaatg gtttaggtct cgcaattaca aaatccatca ttgaattgca tcatggtaca
    25141 atccaattca cacaaagtaa cgcgtatgta accaccttta ctattacact gccaaatcat
    25201 tcacattaat ctctacctcc tgaaagaaca tttctgtttt tatttcagga ggtatctttt
    25261 atattgatta gcgctttcta gccacttaac atttaatttc taccattcaa cacattttaa
    25321 atatatcaat ggtcgtttat ggtttaataa ttttgaagga ggcattaacc tatgatgaaa
    25381 ctcaatttat ttatcaatgc aaatgagacg gaatcttata ttgatataca tgcacctaaa
    25441 atgaatgatc acgttcaaag tattattaat gcagtcaatg atttagacaa atcacataca
    25501 ttagtcggat acatagacaa agaaatccat atcattaatg tatcagatgt tataacattc
    25561 caagttatta ataaaaatgt aacagccatt acaagcaatc aaaagttcaa actaaaatta
    25621 cgactttatg aactagaaaa acaattacca caacatttca ttcgcatttc caaatcagaa
    25681 atcgtcaaca agtattacat tgaaaaatta ttattagagc caaatggact tatacgtatg
    25741 tatcttaaag atgcacacta cacctattct tctagacgtt ttttaaaatc tataaaggag
    25801 cgcttatcaa tatgaaaaat ttgaaaaact cattatttat tagcttaatc attggtttat
    25861 ccctttcact gttctttagt atgttatttg cagacggcaa atattatcca ctgaacccac
    25921 aatcaactat tggcatattg tattatacgc atttcacaga aactaccgta atgttgattt
    25981 ctatcatttt atggttactc ataggcgtcg tctttttcct tggagatttt atctttaaat
    26041 acacagattg gggcattact aaagcaacta tagtgcattt cataacaacg tatgtcggat
    26101 tcttaccttt agcaatactt gccggttggt ttccactaac aataaattac ctaatcatat
    26161 ttacaattat ctttattgtg gtttacacat tgatttggat tattcaattc tttaaaaaca
    26221 agaactacgt agacactatt aacgagcaac tgaaacaatt aaaatgattt ttattaaaat
    26281 tataaaagct atatattgca ttaaagtagt ctcttctgca atgtatagct ttatgtcgta
    26341 tttattttaa atacgcatga actgaataat atatttttac tattttcaaa ttaaattgta
    26401 actgcaactg tcctttacga catcaactca tcatgataaa aacctatcga aatgcatttt
    26461 caaatatttt attcataatt tctgctactt ctttaggact ctctttatat cctcgtttga
    26521 cccaatcttg taacacacca atgattgcat tacacataaa aataagtaaa aaatactttt
    26581 ccttcgtcgt taacattttt gtatttaata ctggtacaaa attaatttcg at
//
LOCUS       DATTQF010000022        24688 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.022, whole genome shotgun sequence.
ACCESSION   DATTQF010000022 DATTQF010000000
VERSION     DATTQF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.022"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(394..498)
                     /locus_tag="VB241_000846"
     CDS             complement(394..498)
                     /locus_tag="VB241_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244164.1"
                     /translation="MLLLERTSMSDFEMLMIVLTIIGLVLISTQDHKK"
     gene            1178..1336
                     /locus_tag="VB241_000847"
     CDS             1178..1336
                     /locus_tag="VB241_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEP1244165.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(1995..2852)
                     /locus_tag="VB241_000848"
     CDS             complement(1995..2852)
                     /locus_tag="VB241_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1244166.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPEDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQIINLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGINQKETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(2920..3702)
                     /locus_tag="VB241_000849"
     CDS             complement(2920..3702)
                     /locus_tag="VB241_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244167.1"
                     /translation="MLIQLDQVGRMKQGKTILKNISWQINEGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGYVNLFGKMPGKVGYSTETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3934..5739)
                     /gene="glmS"
                     /locus_tag="VB241_000850"
     CDS             complement(3934..5739)
                     /gene="glmS"
                     /locus_tag="VB241_000850"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP1244168.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVTLQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6420..7958
                     /locus_tag="VB241_000851"
     CDS             6420..7958
                     /locus_tag="VB241_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HEP1244169.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWFPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFFGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLATLVSFV
                     VAALIMKFTKEPKQDLEAATAQMETTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SLDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            7993..10125
                     /locus_tag="VB241_000852"
     CDS             7993..10125
                     /locus_tag="VB241_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP1244170.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRITGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENYFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNLAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVKKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETRDKSSNMI
                     DKDDVHIETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLISDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10137..10571
                     /locus_tag="VB241_000853"
     CDS             10137..10571
                     /locus_tag="VB241_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1244171.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEGNVDRIVQAKSAEEIKQVFEEADA"
     gene            10571..11677
                     /locus_tag="VB241_000854"
     CDS             10571..11677
                     /locus_tag="VB241_000854"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEP1244172.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALATGH
                     QYDVILADESKTTTRVNNVAAINSMQPSEELKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLAYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(11996..19384)
                     /locus_tag="VB241_000855"
                     /pseudo
     CDS             complement(11996..19384)
                     /locus_tag="VB241_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(19667..21022)
                     /locus_tag="VB241_000856"
     CDS             complement(19667..21022)
                     /locus_tag="VB241_000856"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP1244173.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21049..21981)
                     /locus_tag="VB241_000857"
     CDS             complement(21049..21981)
                     /locus_tag="VB241_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEP1244174.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLVNITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKITGGEEQLNDIAYLKATFKTNKKINGDTKDIAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVSKAEPSETKAYINVK"
     gene            complement(21983..22792)
                     /locus_tag="VB241_000858"
     CDS             complement(21983..22792)
                     /locus_tag="VB241_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP1244175.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(22982..23890)
                     /gene="rocF"
                     /locus_tag="VB241_000859"
     CDS             complement(22982..23890)
                     /gene="rocF"
                     /locus_tag="VB241_000859"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEP1244176.1"
                     /translation="MTKKKGIDIIGVPSTFGQRKLGVDLGPTAIRYAGLIPRLKQLDL
                     DVNDKGDIKVSAVDIEKFQSEQNGLRNYDEIIDVTQKLNKEVSASIENNRFPLVLGGD
                     HSIAIGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLTYRESHFALELLHQSQSVTSMDLVEVN
                     PLIDHNNHTAEQSVSLVGTFFGETLL"
     gene            complement(24032..24104)
                     /locus_tag="VB241_000860"
     tRNA            complement(24032..24104)
                     /locus_tag="VB241_000860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24069..24071),aa:Lys,seq:ttt)
     gene            complement(24108..24182)
                     /locus_tag="VB241_000861"
     tRNA            complement(24108..24182)
                     /locus_tag="VB241_000861"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24148..24150),aa:Gln,seq:ttg)
     gene            complement(24192..24275)
                     /locus_tag="VB241_000862"
     tRNA            complement(24192..24275)
                     /locus_tag="VB241_000862"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24239..24241),aa:Tyr,seq:gta)
     gene            complement(24304..24379)
                     /locus_tag="VB241_000863"
     tRNA            complement(24304..24379)
                     /locus_tag="VB241_000863"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24344..24346),aa:Val,seq:tac)
     gene            complement(24387..24458)
                     /locus_tag="VB241_000864"
     tRNA            complement(24387..24458)
                     /locus_tag="VB241_000864"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24423..24425),aa:Glu,seq:ttc)
     gene            complement(24460..24534)
                     /locus_tag="VB241_000865"
     tRNA            complement(24460..24534)
                     /locus_tag="VB241_000865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24500..24502),aa:Asn,seq:gtt)
     gene            complement(24546..24660)
                     /gene="rrf"
                     /locus_tag="VB241_000866"
     rRNA            complement(24546..24660)
                     /gene="rrf"
                     /locus_tag="VB241_000866"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         7204 a         4474 c         3958 g         9052 t
ORIGIN      
        1 gatgcgcagc atccaactgt gttcctctat ttttcaactt tagttgttag gggcttcttt
       61 ctaaaataac taagtgaatt atctatatat gaagaggtct ccaatacttt ttatataatt
      121 cgtgattaca ttctatactt ttaattgtat tttctgctgt atggaaagtt ggttttatta
      181 attctgatta tgttcagaac tacaattcat aagaaaaatg tgttaaaata tatttgtagt
      241 aagtagaagc aaaagatgaa aatctttaac tcttgaaaca caaaaagggc aacactcgga
      301 aacatgttac cctaatgagc ccgttaaaaa gacggtgacc ttatatttta tttaaaaata
      361 gccttcaaaa tgccggtcaa agcgaataga aggttatttt ttatggtctt gagtactaat
      421 caatactaaa ccaatgattg ttaatacaat catcagcatt tcaaaatcag acatgctagt
      481 cctttctagg agtaacaact ataaccatag gcatcacccc tttattaaga gattagccac
      541 catctatcca acttgctcaa taaatattat aacatgcaca cttttaaaca gtaattcata
      601 gtataagttt tttcatttct taatcaactc gataaacagt tgagtgacat acccttgtct
      661 aactagctat ttcttcccct gttatcgttt gttcatcata taaaaattta atttctcatt
      721 ttttattctc tgataaagaa gagcaccaac cttttcggct gatatgctcc cttcaaaata
      781 gacattgaaa aatgaaaact ttgaaagaga aatcgtcagt ctattattga taagtattat
      841 cctatcctcg aaaaattact ttcagacaca agtgaacaaa aattttacta taagcgtata
      901 ctttggcaat attcgaagga aaaacatgga ttaacctatg catattatac atttagagct
      961 tatatactta aacatgatga atttactcct tatattatga aatgttatca atagatgtcg
     1021 cctaaagata caacaagatt cgagacaaaa cctgatcatc aagcacagtt tgactggaaa
     1081 gaaagcatta actttaaaac gaaagataat caaaggattc cactatatat aggtgtgcta
     1141 ctactttatt attctcgttt tataatcatg tatgacaatg agtaaatcaa gtgatgcctt
     1201 atttaattca ttaacacaat cgtttgagct aatagaaggt gtatataatg aacttgtaac
     1261 ggataacttg aaaatagtca tgtataagcc aagaaaagaa agtacaagtg gtcgaataaa
     1321 tcgtagattt aaataattcg ctgatggttt taaatttcaa gtaaaacctt gtatttccgg
     1381 cctccctaga aaaagataaa gttgaatcaa taacaaaaat attagatgaa attaatgctt
     1441 accaagacga attgcatttg gaagagattc ctaagttttt ttaatcttaa tgaacgattt
     1501 aactactaag ttcatactag tacaggtaaa ttgcctatta ctgcctaaga gaaagaaaaa
     1561 attttcttac aaccactaca gaatgtgaat gtaaaaaact catataaagt gaaacatatc
     1621 ttacttatgg aatgaaatgt ttgattatcc caaaatcaca aatgcgattt tggagttgtt
     1681 aggggctcta acgcaaatac actcctactt aaaataacgg tagttgttat agtatactta
     1741 aatgcataaa ccatataaaa ggtgatgcta atatgacata cggttcaatt gtagctatac
     1801 tctgtgccct atttgtgatt ttcttcattc catatatgga gaagaaagac aaaaagaaga
     1861 aataacacta gtatctggat tactagcata caaaggagca gtaagacatt ttctaataga
     1921 aaataacttt actgctcctt tgtaaattac tcttcaacta ttgtaaatta aataataaat
     1981 aactgccgat gttattaatc agctaaaaaa tgctttgcta aatattttgc tgcgccatcc
     2041 tcttcattcg tataagccgt tacatctgaa gttaatgctt ggatttcagg acgtgcattt
     2101 ttcatagcga ctgtgtaatg gccaaattca aacattgctc tatcattgtc gctatctcca
     2161 ataactaacg tttctttttg attgatgcca aaatgttcaa tcatttcttt aatgcctgta
     2221 cctttatcag tttgataagc cattgtttcc gcattaaatc ttgatgaatt tgaaacacta
     2281 atctgtagtt gcacatgatt ttgctttaat tcatctctaa atgctgttat tttttctaaa
     2341 ttagaactga acagataaat ttttgaatat tcaccttcag gaaacttagt tacccaatct
     2401 atcttaccag caagcgcatc ttgtcttgaa gaccattcac tatgacttac gccattaata
     2461 ggatcttgac tacgaatcat atctcgcatc catgtttcat cttcttttaa agaaactcta
     2521 ttaccttcaa aaggaaatac ctcataataa atttgttggc gcttagctaa attaataatt
     2581 tgttgcactt gagccaatga taaaccatgt ttgaagataa tttctccatc tacttctcca
     2641 atggttccat ttgaactaat gatgccattt accgcaaaat cttcaggtac aagttgatgt
     2701 atttcagaat gcgaacgtcc agtcgctaaa aatactttat atcctttctc tctcaatcga
     2761 ttaatgacat ctttcgtata cgttgatgct tgattatttt catgtaaaat tgtaccatcc
     2821 atgtccaaaa atattgcttt tacgttatcc atattgccct ccactgtcaa ttttagctta
     2881 attaaatttt gtcattatta gttagtattt gcaaaatatt tactctaaca tcgccataga
     2941 aaatcgatta ttccatcttt gaactgctac atttttctgg aaaaatcgtg acatgttttc
     3001 agatgtcaaa atatcatcta ttaacccttg ttgtacactt tccccatctt ttaatagcaa
     3061 aattttagta aagttaccag taatttcttc aataaagtgc gtcacataaa tcatcgcaag
     3121 cgttggatat gaatctgaca atgagtcaag tatacttaac aacgattctc gtgcaataaa
     3181 gtctaaacca gctgctggct catctaaaat taaaacctgg ggttgcccca ttaaagctcg
     3241 cgcaatcatc actcgttgtt tttcaccggt agataaataa ccaatatatt gttgtgcttt
     3301 agcagacatt ccaactaatt taagtaattg atgtgcttca ttacgtacct catcatcaat
     3361 atcttgataa acaccaattg atttaaaagc accgcttatc actacatcga ttactctttc
     3421 accctcttga aacttttcca gtaaactatg agatacaaaa cctatatgtt gtcgtacagt
     3481 ttctgtagaa taccctacct tgccgggcat tttaccaaat agattaacat agccagttgt
     3541 cgcaggctca tacgcattta aaatatttag aagtgttgtc ttgccagcac catttaaacc
     3601 atatagaatc catttatcac cttcattaat ttgccaagaa atatttttta aaattgtttt
     3661 tccttgcttc attcgcccaa cttgatctaa ttgaattaac atgcgatgcc acccctttat
     3721 tgctactcca tacttctcat aaaatcatat taacatatca ctcttaattg tcagactatt
     3781 taaataaata aactacttca tttttacgca tttctgccga attaagatga agtaaacgct
     3841 aagtcgacct aaaaaagcac ccttttagtc gattaatcta aaagggtgcc atatacttta
     3901 attttaatac atgattgatt ctaaaaaagt gaattattcc acagtaactg atttagcaag
     3961 gttacgtggt ttatcaacat ctaaatctct gtgtaatgct gcatagtatg aaattaattg
     4021 taatgtaaca actgatacta atggcgttaa taattcatgt acatgaggaa tgacataagt
     4081 gtcgccttct ttttcaaggc cctccataga aataatacat gggtgtgctc cacgtgctac
     4141 tacctcttta acattaccac gaattgataa attaactttc tcttgtgttg ctaaacctac
     4201 aactggtgta ccttcttcga ttaaggcaat tgtaccgtgt ttaagttctc cacctgcaaa
     4261 accttcagct tgaatgtaag agatttcttt aagttttaac gcaccttcta aacttacgtt
     4321 atagtcaata gtacgtccga taaagaatgc attgcgtgtt gtttctaaga aatctgtagc
     4381 aatttgttcc atgattggtg catcgtcaac aatcgcttca attgctgttg ttacttttgc
     4441 taattctctc aataaatcaa tatctgcttc acgaccgtgc tcttttgcaa cgatttgaga
     4501 caagattgat aatactgcaa tttgtgcagt atatgctttt gtagatgcaa ctgcgatttc
     4561 aggacccgcg tgtaataaca atgtgtggtc tgcttcacgt gataaagttg aacctgcaac
     4621 attagtgatt gttaatgatt tatgacctaa tttattagtt tcaactaata cggcgcggct
     4681 atctgcagtt tcacctgatt gagaaatata aacgaacaaa ggtttttcag ataataatgg
     4741 catgttgtag acaaactcag atgcaacgtg tacttcagtt ggtacgccag cccatttttc
     4801 taaaaattct ttacctacta aacctgcatg gtagcttgta cctgctgcaa taacgtaaat
     4861 gcggtctgct tctttaacat cattaatgat gtcttgatca attttcaagt taccttctgc
     4921 atcttgatat tcttgaataa tacgacgcat tactgctggt tgttcatgaa tttcttttaa
     4981 catgtagtgt gcataaacac ctttttcagc atctgatgca tcaatttcag caatatatga
     5041 atcacgttct acaacgtttc catctgcatc tttaataata acttcatctt ttttaacaat
     5101 aacgatttca tggtcatgga tttctttata ttcgcttgtc acttgtaaca ttgcaagtgc
     5161 gtctgatgcg ataacattga aaccttcacc aacacctaat aataatggtg atttattttt
     5221 agcaacatag attgtgtctt tgtcttcagc atctaataaa cctaatgcat atgaaccatg
     5281 taataatgac acaacttttg taaatgcttc ctcagttgaa agtccttgat ttgaaaagta
     5341 ttcaactaat tgaacgataa cttctgtatc tgtttctgaa atgaatgata caccttgtaa
     5401 gtattcacct tttaactctt catagttttc aataacaccg ttatgaacta gagtaaaacg
     5461 gccatttgat gattgatgtg gatgagagtt ttcatgattc ggtacaccgt gtgttgccca
     5521 acgtgtgtga ccgattccaa caggaccatc aaaatcgcta ctatcagcaa ctttacgtaa
     5581 ttctgcaata cgaccttttt ctttgaatac agttgtatta tcatcattta ctactgcgat
     5641 acctgcagag tcataacctc tgtattctaa tttttctaaa ccttttaata ataattcttt
     5701 ggcattatca tagccaatat aaccaacaat tccacacata acgacatttt cctccatatt
     5761 ggaatagtac gcgtaaatta tgatttattg ccgataattt agattgacaa tctgctttca
     5821 taatataaat aggaacatgc tatcatcgca ttcatccata acaaattaag catagttatt
     5881 tttacaacta tgcaaattgc tcacactgta ctttccatat taatattttt tatattcaat
     5941 ttctggcgat cttattaact ttgtccatta agtcacccta atgttttact taataagcta
     6001 acgaatgagc cacatccggg atagcatccg ccgatctatt cgatcactat cctcctcgtc
     6061 tacaaataca tattttgcac tctataaagg ccactcatat attaaccttt aatcttcaaa
     6121 tacaaatatt tatttgcaca ggcgctttaa ctgtactgcc gaactttccc cctttccatt
     6181 aatcattatt gtacaacggt gttgttttgt tttgcaaata ttttcacaat aaaattttaa
     6241 aaatcctaaa acaatttttt tgttttactt tttcaaaata tctacgcagt cacattgatg
     6301 acactttatt taattttgtc acatttattt tgacaaagtt gatttttgtt tatattgagg
     6361 attgcgttat ggctctttac actaaagata gtcacataca ttaaaaaaga ggtgtaaata
     6421 tgtcgcaaac tgaagagaaa aaaggaattg gtcgtcgcgt tcaagcattc ggatcatttt
     6481 tgagtagtat gattatgcca aacattggtg cattcatcgc ttggggtttc atcgcggcca
     6541 tttttataga taatggttgg ttcccgaata aagatttagc tacgttggct ggtccaatga
     6601 ttacttattt aatcccacta ttgattgcat ttagtggcgg tcgtttaatt tatgatttac
     6661 gtggtggtat tatcgctgca acggcaacta tgggggtcat cgttgcatta cctgatacac
     6721 caatgttact tggagctatg attatgggtc ctttagttgg ttggttaatg aaaaagactg
     6781 accaattgat ccaacctaga acaccacaag gttttgaaat gctatttaat aatttttcag
     6841 ctggtatttt aggatttatc atgacaattg caggatttaa aattttagca ccacttatga
     6901 aatttatcat gcatatttta tcagtggcag ttgaagcatt agtacatgca cacctacttc
     6961 cacttgtaag tattttagta gagcctgcaa aaattgtatt tttaaacaat gcgattaacc
     7021 atggtgtatt cacaccgctt ggtgcagatc aagctgcaaa agctggtcaa tctattttat
     7081 acacaattga atctaaccct ggaccaggtt taggaatttt acttgcatac atgatttttg
     7141 ggaaaggtac tgcaaaagca acttcatatg gtgctggaat tatccacttc ttcggtggta
     7201 ttcatgaaat ttatttccca tatgtattaa tgcgtccttt attattcatc gctgttattt
     7261 taggcggaat gactggtgtt gcaacttatc aagcaacagg atttggattt aaaagtcccg
     7321 cgtcacctgg ttcatttata gtttactgtt taaatgcacc tagaggtgaa ttcttgcaca
     7381 tgttactcgg tgtcttcctt gctacacttg tatcatttgt cgtagcagct ttaattatga
     7441 agttcactaa agaacctaaa caagatttag aagctgcaac tgctcaaatg gaaactacta
     7501 aaggtaaaaa atctagtgtt gcatctaagt tagtatcttc tgataaaaat gttaatacag
     7561 aagaaaatgc tagtggtaat gttagtgaaa catcttcatt agatgatgat cctgaagcgc
     7621 tattggataa ttacaacact gaagatgttg atgcacacaa ttacaataat ataaatcatg
     7681 ttatttttgc ctgcgatgcg ggtatgggtt ctagtgcaat gggtgcaagc atgttacgta
     7741 ataaatttaa aaaagcgggc attaatgata ttacagttac aaatactgca attaatcaat
     7801 tgccaaaaga tgctcaatta gttatcactc aaaaaaaact aactgatcgt gcaattaaac
     7861 aaacaccaaa tgccatccat atttcagtgg ataatttcct taattcacca agatatgaag
     7921 aacttttaaa taatctaaaa aaagatgatc aagcataata attaaataaa ttaaaaaatg
     7981 gaggataccg ccatgttatt gagtacacgt gaaaaagaaa tgatagccct attgattaag
     8041 taccacggtc aatatatcac tatacacgac attgctcagc aacttgcggt gtcctctcgt
     8101 actattcacc gtgaattaaa aggtgttgaa gcatatttaa cttcattttc attaacttta
     8161 gaacgcgcaa acaaaaaagg gctacgcatt actggcgcag attctgattt aaacgatttg
     8221 aagcaatcga ttgcacaaca tcaaaccatt gacttatctg ttgaagagca gaaagtaatt
     8281 attatatacg ctttgataca agccaaggag ccagttaaac aatatagttt agcgcaagaa
     8341 attggcgttt ctgtccagac tttagcaaag atgttagatg atttagagct cgatttaaat
     8401 aagtaccaac tatctttatc tcgaaagcgt ggcgaaggta tttacttggt aggtactgaa
     8461 tcaaagaaac gtgaattttt aagtcaatta atggtgaata acttaaatag tactagcgtt
     8521 tattcagtaa ttgaaaatta ttttgtcttt cattcattaa atcaaatcca caaagacttt
     8581 gttgacttag agcgtatttt taatgttgaa agactattaa tggactacct aagtgcctta
     8641 ccctaccaac ttaccgaatc aagttattta actttaactg tccatatcgt gctatccatt
     8701 tcacgtataa aaaatggaga gtatgtcgca ttaaacgatg atatttatga ttctgtacaa
     8761 aacacatttg aacacaaagt agcaagcgaa cttgctgata aacttggtca aatatatgac
     8821 gtcacgttta atctggcaga aattgctttc attactatcc atttacgtgg tgctaaacga
     8881 aaaaatctta atgatacatc attaaataat cgttgtgaag aaaacaaaat taaagcgttt
     8941 gttaaaaaag tagaaatgat ttcaggtatg acatttgcag atttggatac tttagtagat
     9001 ggactgacgc tacaccttaa tcctgcaatc aatcgtttgc aagctaatat cgagacctat
     9061 aatccgttaa cagacatgat taagttcaaa tatccaagac tgtttgaaaa tgtaagatta
     9121 gctttaaatg attgttggcc tgatttgatt tttccagaga gtgaaattgc ttttatagtt
     9181 ttacactttg gtggctcgat taaaaaccaa ggtaatcgat ttttaaacat attagtcgtt
     9241 tgcagcagtg gtatgggaac tagccgtcta ttatcaactc gtctagaaca agtttttagt
     9301 gagattgagc gtattacaca agcatcagtc agcgatttga agtcactaga tttaagtcaa
     9361 tatgatggca ttatttctac tgtgaattta gacatagact ccccctattt aacggtaaac
     9421 ccattattac cagatagtga tatcagttat gtcgcacagt ttttaaatac aaagtctacg
     9481 ttccaagaga cgcgcgataa atcatcaaac atgattgata aggatgatgt tcatattgaa
     9541 acgaaagata ttgatggcaa cacatctttt gaaaatgaac aaacttcata cttaacttca
     9601 gttttcgaaa aacatttaag tgacgaaaaa tcagaacaat taatgcatca tatgcgttcg
     9661 ggtttaactt tgcttgattc agtgaaaata gttagtaccg aagttaaaca gtggcaaaca
     9721 tatatcgcag attatctata tcaatgcgat gtaataaacg atccaacgtc attcgctgaa
     9781 ctactagagc aacgattgat tgacaatcca ggatggatat taagtccata tcctgttgca
     9841 ataccacacc taagagacaa tatgattaaa caccctatga ttctaatcac agttttagaa
     9901 gaaccgttaa cattgcctag tattcaaaat gacaatcaaa caattaaata tatgatttcc
     9961 atgtttattc ctgacaatga ttttatggca tcactgataa gtgacttgtc cgaattttta
    10021 agtttgaaat tagaatctat tgatactttt atggaaaatc cacaggaact tgaaacatta
    10081 ttaagaaaca aatttttaga acgaattaaa aaacaattta tttaggagtg tacaaaatga
    10141 gcgaattatt tagtaatgac aatatctttt taaatgtaaa tgttaacagc caaaatgaag
    10201 caattgaaaa agcaggtaaa gccttagttg atagtggtgc tgtaacagat gcttatattc
    10261 aagcaatgaa agatcgtgag caagtcgtat caacatttat gggaaatggc ttagcaattc
    10321 ctcatggcac agatgaagct aaaacaaatg tgattcactc aggtttaaca ttattacaaa
    10381 tccctgaagg cgttgactgg gatggcgaag tagttaaagt tgtcgtggga atcgctggta
    10441 aagatggcga acatttagac ttgttatcta aaattgcaat tacatttagc gaagaaggaa
    10501 atgtagaccg tatcgttcaa gcaaaatctg cagaagaaat taaacaagtt ttcgaggagg
    10561 cagatgcata atgaaagcag ttcattttgg tgctggtaat ataggtcgtg gatttattgg
    10621 atatattcta gcagataaca atgtaaaagt aacatttgca gatgtaaatg aagaaatcat
    10681 taatgcatta gctactggac atcaatatga tgttatttta gctgatgagt ctaaaacaac
    10741 aacacgtgta aataatgttg cagcgattaa ctcaatgcaa ccttctgaag aattgaaaca
    10801 agcaatttta gaagcagata ttattacaac agctgttggt gttaatatac tacctattat
    10861 tgctaaatct tttgcgcctt tcttaaaaga aaaaacaaac catgttaata ttgtcgcttg
    10921 tgagaatgct attatggcaa ctgatacatt gaaaaaagcg gtacttgata ttactggacc
    10981 tcttggtaac aatattcatt ttgctaactc agcagttgat agaattgtac cattacaaaa
    11041 gaatgaaaat atattagacg ttatggttga gccattttat gaatgggttg ttgaaaaaga
    11101 tgcatggtat ggtccagaac taaaccatat taaatatgtt gatgatttaa caccatatat
    11161 tgagcgtaaa ttattaactg tgaatacagg acatgcatat ttagcgtatg ctggaaaatt
    11221 tgctggtaaa gctacagttt tagacgctgt aaaagatagt tcaattgaag ctggcttacg
    11281 ccgtgtttta gctgaaacaa gtcaatatat tactaatgaa tttgatttta ctgaagcgga
    11341 acaagctggt tatgttgaaa aaataataga ccgtttcaac aattcttatt tatctgatga
    11401 agtaacacgt gtcggacgag gtacgttacg taaaattggc cctaaagata gaattataaa
    11461 accattagca tatctttata ataaagattt agaacgcact ggtttattaa atacagctgc
    11521 attgttattg aagtatgatg atacagcaga ccaagaaact gttgagaaaa ataattacat
    11581 taaagaacac ggtttaaaag cgttcttaag cgaatatgct aaagttgacg atggcttagc
    11641 cgatgaaata attgaagctt acaattcact ttcataattt attgagcttt gtttgaaact
    11701 ataattttcc aacgttattc gttaacaatc agtaataatg tagtagttcc gttgaattaa
    11761 caatattaca tttctgaaca taaaaaatac tcccttcaac atagacactt aacttgtgtt
    11821 atgtatgaaa ggagtatttt tgcgttaata atttgtttta ttttcgagcc acagccacct
    11881 attcaatggc tattggtcat tactaaaaca aattcatatt aactgttaga cttggttact
    11941 tagtaaggaa tatttcccta tgaaataact agatgttcac attcttaaat aaattttatt
    12001 cttcagtttg ttggtctttc ttagtgaatc ttctaattaa gaatgccata cctgcaccta
    12061 gagctaattc aacatatggt aaatcgtcat tatgtgacat accagtatct ggtaaagttt
    12121 tagcttgttg ttttgcttta ttttctttac cttgttgcgc tgatgttgcc ttagcttggt
    12181 gtttgtcagt gttagttact ttaagcatat cttgattagc actattgctt ccatttgaaa
    12241 ctgtagctgg agatgcattg gcaccgtcgt tttgcgtagc tttattgttt acatctgcag
    12301 ttgcaccaat tgctgcttga ttatcttttt tatctgatgt tgccatgtca gccttttgac
    12361 tatcctcagt cgaagtcgct ttatcttttg cttctgacgg tttagatgtt gatggtttat
    12421 tcgatacatc agtatcaacg ttgccttcag atttatctgc ttcgttagat gcaacgttag
    12481 tttcagactt aagttgtcca gcatctgttt gatttgtcgt actttctttt ttatctttct
    12541 ctgtattaga aggtactttt ggttctgtaa tgtctgctga aggcaatgtt tcagttgttg
    12601 attcaaccat actttgattt gttgaatctc taccatcttt ttcatcctta gctttatttt
    12661 cagattttgg ttgtgcaacc ttgccattag ttgattgaga ttcagcacta ttatttactt
    12721 cagcattttg ttttgaatca tttacagatg cattatcttt gctatcagca gatgatgctg
    12781 cacctgtgct cgcatttgtt gttggaaccg ttgctgttga tcctgttggt gcgttctcgt
    12841 ttgttgcagt tgtactattg ttatttcttg cgctttctgc tggcgttgca ttatctgttt
    12901 ctgttgcaga tttatcagtt gtgccgttat tagttgattc tacttctgat ttatcacttg
    12961 taccattacc cgttgccgaa ttgtctgttg atgttgctgt tgttccacca gttgccgcat
    13021 cattatgatt atttgtcgtt gttgaagacg tatctgcatt ggcattatct gttgactcag
    13081 ttacattagg ttgtccagta ttttctggtg ttgcattagc atttgaattt gctgttgcat
    13141 cattattatc tgaaccacta ttagtatcat tagcatttgg atccttctga ggtacaatcg
    13201 cttcaattgc aggtatcgtt acattttgta attcatcaac ttctgtattt gtttgcgttt
    13261 tatctaattt atcagcaaat ctgtcaaaat atctacctaa atctgtacgt gcaatttctt
    13321 tcgccgatgc atctgcattt tcaattattt ctatttgctt gttaaccact tctctaattg
    13381 cttccaaagc atttttctta actactggat taatacgttg tgctttaagt tgttcaagcg
    13441 cactattttt gacagtagcg atttctgcat ttgtagtttg atcagaaata tcttcagttg
    13501 cttttgataa aatgtcttct aatgcattcg taaacgcttc tttttcttca gttgtagcat
    13561 cagcgtcgac atttactcct gcttcaatct agtctagtgc agtttctaat tcttcgatag
    13621 ctttttgttt ttctgttgag tcaatttgaa tgttatcaaa cgcctcaagt ccttgagctt
    13681 tcgctttttc aacttcagca gttgttgttg catcagtaat accttgttta gcttgatctg
    13741 taatttgttt aatcgttgct aatgcagccg ttttttcttc agcagttaac tggtcacttt
    13801 ggtctataga ctctttcgtt tcctgtgctt tagcatcaat cgcttggttt gctttaggtt
    13861 taacagtagc atctacttga actgcatcaa ttgtcgcttt accttgtgtt aatgcatcat
    13921 caacatcact attatccaca ccattattaa tgctttctaa tgcagtttga acattttggt
    13981 caacttgctt aattgcttgt tgtttttcat cttgtgttgc attagtgtca gctaaaatat
    14041 tatttttctt ctgatctgca taggcatata aatctgttgt agccgattct tttttaccgg
    14101 ttggaattgt gtaatcgtta atattatcta agtcattatg aatttgaact tcaatgtcat
    14161 ctttagaagt agcttgatta acattttgat ccgctgtttg ttttaattca gcaagttttt
    14221 gtttcgcttc agcaatttca cttgaagtcg atgcgttaga gttatccgct tcgttcactt
    14281 tagcgttata tgcatctgca attttagcta aagcatcttt tttgtactca ctaaatgttt
    14341 taactgcatt aattttagct ttcccttctt taaccgcatt atcaacatat tcatttgttg
    14401 atgattgatc aatatttgtt ttcgcttgat ttaacgctgc atcaacttct tgttttgcat
    14461 cattaatttc ttgttgtgac gcatttggtg tttgttcaat ttgtgttttc ttatcagctg
    14521 cagcttgatc taattctgtt ttacctgctg gtttcttaac aggatttgca tgaagttgtt
    14581 gaactttttg tactgctgta tctttagcag tagttacatc acctgtagta gttgctgcat
    14641 taatattatt taaaccttct tcatatgctg ctctaactgg ttctatatct tttccttttt
    14701 cttcatttgt agtcgtatta tcattaagga tttcagttat tttattttgc atttcattta
    14761 gcaattccgc tcttgcattc gttttaacat ctgttgatgc ttgaattggg tcaattgctt
    14821 gaattgcatt atcttttgca gtgtttacat catcgattga ctttgcattt tcaatatttt
    14881 gattaccttg cgttaattgt gcatctactt gttgatttgc ttgttctttt tcttcagtag
    14941 tcgcatctgc agtttgtgca ataagtgctt tttgttcctt tgcttttgtt gctaattcat
    15001 ctttagctac atctttaatt gtcgtatcag cagtaatacc ttgaatgtca gcaactgctt
    15061 gatctttaat ttgtgtaaca tcgttagtcg tttgcgcatt taaaatatct tgatatgctt
    15121 tttctttagc tttagaaact aaatcttttg ctgcattttt ctcttcagtc gttgcacctg
    15181 tagtattatc aattgcttga ttttgagttg tcacagcttg atctacttca tttttcgcat
    15241 tcgatttaac tgctgttgct ggttgtgtac tttgaatcga atttttacct gcatctttag
    15301 cagtgtctac accattattg tcagttgcag ctgtaatgtt atttttcgcg tctgtaactg
    15361 ctgttgctag ttgttgaatc gccgcttctt tttcttcatt tgttgcgttt tgatcgttat
    15421 taataaccgc tgtttgtgct gcttgtaatt gatcgatctc atctttcgct gcttgtttct
    15481 tcataacttt tggtgttaca gcgttaattg ccgcttctgc attagttttt gcctcatcta
    15541 cttctgcatt tgttgtagct gctgtgatgg cttgatttgc tttagcattt tcagtattag
    15601 cttcagtttc tgctgcttgt ttctcttcat ctgttgcatc tggcgtagct tgaattgctt
    15661 gtaacttgtt atttaatatt gtagtgattt cattacgtgc tgtagctttt ttattaacag
    15721 ttggtgtcac ttgatcaata ctagcttcac ctgttgtctt cgcttgatca acttcatttt
    15781 gactattagc agtctcaatg tggttgtttg cttctgttac tgcattatct acgttcgcat
    15841 tagccgctgc aatttcttct gctgtaatct cttgcgtttg agcaatggct gctttacgtt
    15901 cattcgcttt cgtagcaatg gcttgtttcg catcatcttt cgttgttgtc gctggttgaa
    15961 ttgcttcaat tttagcaatt tctgcattct tagccgcttc aacttctgca tttgaatgtg
    16021 caccatctat tgctgtatca gctgtcgttt tttctgtttg aacttgttgt ttcgctgctg
    16081 ttttctcttc tgttgttgca ccgttattag catcaattgc tgtttcttgc gcttgtactt
    16141 tatctgcaat tgcttgtttc gcttgaggtt taacatttgc atcaggtgta atggctgcga
    16201 ttgtagcttc atttgtagtt tttgcattat ccacatcatt gtttgctgcg gcattatcaa
    16261 tatcagcgtt tgcattaact actgcttgat ccactttatc tttcgctgct tgttgttctt
    16321 cagtagtcga atcattcatt gcttcgattg cagttttacg ttcacttgct ttttgagcga
    16381 tttctgcttt agcatccgat ttcttagtag tggcagcttg aacttgatta attgcagcaa
    16441 taccattgtc tttagctgtc gttacagcac tgtttgtatt tgcagcatca agattcgttc
    16501 ttgcctcttg tttttttgca tctaattgcg tatatgccgc ttctttttct tctgttgttg
    16561 aagtgttact attttggatt tcttgtttac gtgtattata tgcgttgtct acttctctat
    16621 ccgctgcagg tttaactcta gcttgtgttt gaatggcatt gattttatct aatacttttg
    16681 ctttagtatc agccacttcc tgttttgatt taacaacttg gatgtcatgt aaaccttctg
    16741 tttgagctgc atctactgct gcatcagctt ctgctacttc ttcatcagtt gcatttgaaa
    16801 ctgtagcatt ttgagctttt ctagctgtag cagcttgttt aacttcatta taggcatcca
    16861 tttttgtttg cgtagagtcc gtaatattct caattttgaa tatttcttcg tttttaactc
    16921 tatctaaatc ttgtgcagtc gttgtcgctt caattgcttt aacacctgaa actttagctg
    16981 cattaatacg ttcaatcgct tccgctacct cttcatttgt tgtatcagga tttaaaggtg
    17041 cttgatcaat ttgtgcttga acaacttcgt caaattcttc aagagctgct gctttagctg
    17101 ttgtagcagg tgcagtttga ttaatatcat tgatcgcttt tgttttaatg gcttctactt
    17161 gtgcatttgt tgttgctgca tcaatatctt taattgcctt tgtttcaatt ttaccaattt
    17221 tatcatttgc tacatctttt tcatcttgta atgatgcatt tgacttttta atttgttgtt
    17281 tacgagttgt aactgcttgg ataatatctt gtttcgcttg aggtttaact gttggtgtaa
    17341 tcacatcttg ttccaacact gcgataccat tatctttttc agttgtgaca ccttgtgctg
    17401 ttgtttgttt attaatttct gcgattgcat tatttttatc attgttaatt ttagtcacaa
    17461 gcgctgcaac ttcatcttgc gtaacttttt tactttgttg tgctgctgta accttttctt
    17521 gagctttagc atctaattca gaaattgctg cttgattatc aattaatgca gcttgtaatt
    17581 tagttactaa accatcaata tccgcttgag atgcacgatt tgcagtttca acatgattcg
    17641 catcttcatt taaaatagtg tctgcttgtt gtttaagttt attgtattct gcaattgatg
    17701 ctgttgtgta atgactatca tcaacttgtg agttaacttg ttgttgcaac gcatctttgt
    17761 tcatttcaac tgctactgaa aatggatctg cagttagtgt aacttctggt tgcgcattat
    17821 taattacaac atctgaagca gtacgatatg ttaatttttc attaaaatca atatttttag
    17881 gtgtatcaat attcgcaaca ttaactttat atgataattt taaagattta tctgggaata
    17941 acacttcttt cgtatgtgta ccacgtgctg ttgtcacacc ttggcttgta aatgtaactt
    18001 tatttgcatt ttgatcataa ttgacagtca tatttttcaa tactgtactg tcttcattac
    18061 cattagggaa tgttgtagtt aatgaatcat taacgtaagt tacaccttgt ggtaatgtta
    18121 cttcatattt aaattgatct gctttcaatg aagcaccaaa attaccattg ttaccgattt
    18181 cagtattaat agtatattct ttattgttag ttgctttcgg tgctaaatct ctatttttga
    18241 catataaatg tgatcctgag aatagaccta cattatcaat gaatgaatag tatttataac
    18301 catctttatt tgttgtaatt cttgaagcat cagcaactgc tgtattgtca ggaataaatt
    18361 ctaaatctaa acgatcagca tctgttggta ctttaaataa gtgtgcagta ccagtcttag
    18421 ctacatttac agttgctaat tcagtatttt tcttagcatc tttaataact aaatttgttg
    18481 caccttgctt actattcgtt tgcatagtag taaagttgat aattaattct gaatctggtt
    18541 ttacattaac agtttttgaa ataccgttaa agttaccgtg atctgtagaa tcatttgcat
    18601 tcacacgacc taatgcagct acattccctc tagcttggta gttttgaggg ttattcttat
    18661 caaacatatc gcttcgtctt aattctgagt taacgaaacc aatcttaccg ttgttaatta
    18721 atgaataacc atttacttta tctgtaacag ttacagttgg atcctgtcta ttctcatctg
    18781 ttgatatggc aggatcatca aacgttaatg ttgtattaat actgccttca ccagtattgc
    18841 tagcatttgg atcttgagtt tgtgcgtttg ctgctacagg tgctgctggt tgcgctgctg
    18901 ctggagcatt cgctggctgt gtttgatttg ccggtgttgt attattatta ggtgttgctt
    18961 ggttatttcc ttgacctgct tggttggcag gtgctgcttg atttccaggt tgtgcttgtg
    19021 cagcgttatt cggatcagct tgattacctt gtccagctgg ttgtgctgta cctgctgccg
    19081 gttgtgctgc gcctcctgct ggattagcct gtccaccttg gtttgctggt tgtgctgctg
    19141 gttgtccttg gttggcaggt gcagctggct gtgctgtagg attagcttga gcaccagcat
    19201 ttgcgttagg ctgtgcattg gcatcagctg gttgtgctgg ttgattttgt gcaggctgat
    19261 tttactctgc tgcagacgct gttgtcgggt taatagatat aaatgtaaca gtggcaatta
    19321 aagctgaaaa aataccgaca ttaaattttc taatactaaa tttttgttgt ctgaataaat
    19381 tcattaagtc atcctcctgg ttgattattc tcgctgttaa atgatgtcac ttaatcaact
    19441 tttaagataa acagtttgct tccatcttat aaaaggtaga gcaactctat ctaattaaaa
    19501 ttaattttat catcattata tattgcttac cagtgaattt tatattacat attgattact
    19561 ttgtttttat gtattcttta ttattttgac tttacgaaat aatattcttg ctttagtcaa
    19621 attaaaaaac cgatgcataa gcatcggttt gatttatatc ttgaaattat ttatctaatc
    19681 ccattttatc ttgaaccaca tcagctattt gttgtgcaaa tctttcagca tcttcatcag
    19741 ttgctgcttc aaccatgaca cgaactaatg gttctgttcc agaaggtctt actaaaattc
    19801 gaccttctcc attcatttct acttctactt tagtcataac ttctttaacg tcaacatttt
    19861 cttcaacacg atatttatct gttacgcgta cgttaattaa tgattgtgga tattttttca
    19921 tttgtccagc taattcactt agtgatttac cagtcatttt tattacagaa gctaattgaa
    19981 taccagttaa taaaccatca ccagttgtat tgtaatccat cataacgata tgtccagatt
    20041 gttctccacc caagttataa ttaccgcgac gcatttcttc tactacatat ctgtcgccaa
    20101 ctttagtttt attagattta attccttctt gttcaagcgc tttgtaaaaa cctaagttac
    20161 tcataacagt agaaacaatc atgtcattat tcaattcttg atttttatgc atttcttgac
    20221 caataataaa cataatttgg tcaccgtcaa cgatttgacc attctcatct actgctatga
    20281 ttctgtctcc atcgccgtca aatgctaacc caaaatcact ttcagtttca acaacttttt
    20341 ctgctaattt ttcaggatgt gtagagccac atttctcatt gatattatat ccatcaggac
    20401 tacatccaat tgtttcagta tctgcctcta aatcaccaaa taagaatggc gctagtgatg
    20461 atgttgaacc atttgcacca tctaaaacaa ttttcaaacc ttcaaagtta acatctactg
    20521 ttgattttaa ataactcaaa tatttttgtg ccccttcaaa gtaatctgaa taatgtacaa
    20581 tatcattgcc aactggtctt ggtaattctg ggttttcttg atccaataat gcttcaattt
    20641 cattttcttg ttcatctgat agtttaaaac catctgatcc aaagaattta ataccattat
    20701 ccgcaactgg attatgagag gctgatatca ttacacctaa ctctgcaccc atatcgcgtg
    20761 ttaaatatgc aacacctggt gttgaaataa tacctaatcg catcacttct gcaccaattg
    20821 aaatcaaacc agctattaat gctgattcta acatttcacc tgaaactcta gtatcgcgac
    20881 ctacaagtac acgtgggtgt ttttcacctt tattatgtgc tagaacatag ccaccatatc
    20941 ttcctaattt aaatgccaat tcaggtgtta gttcttggtt tgcgacacct cttactccgt
    21001 ctgtaccaaa atattttccc attgtttact ctcctttaat ttagctattt attttacgtt
    21061 tatataagcc ttcgtttcac ttggttctgc tttagagaca tgttctggta aattgatttt
    21121 tacagtcttt tcagttgatt ctgaaatacc atctaaatct acttctgcat caacttcgct
    21181 tatattttgc aagtcatctc gactaccgaa gatttcaatt tctttatctt ctaagtcaat
    21241 cgaacttaat tctttatcat ctgctaaact acctttctgt ttaacattta ctttaacctt
    21301 tttgctaaaa ggctctactt taacttgtaa attcacttca ttaggttgaa tcgatacatt
    21361 taatttattc agttttttat caaaagccgt tacttccgcg atatcttttg tgtcaccatt
    21421 aatcttttta ttagttttaa aagtggcttt taaataagcg atatcattca attgttcttc
    21481 tccacctgtt attttaactg tttgtggtga aacttcttgc tttgtaattt tataaagtgg
    21541 atcaatatca ctttgactaa catcaggttg aactgtcatc tttttagtta ctttgttttc
    21601 aagcgtaata tttaccaatt taggttttac agaataatga atgtcatcag ctaacccttt
    21661 aacttgatac ttctcttgat actttccagc tttagcattt gataaatcaa tcactactct
    21721 taaatcttct ggattttcaa tttttattat ctttgattgt ggtcctgaaa tagtcacatt
    21781 aactgtttca ggcgcttttg ttaaatgcaa atctttagtg ttataaagaa tttcaacggg
    21841 tacatcttga atcgttttac tagacttttg accaagatta ccagtgttaa agatatttcc
    21901 aaaaacattg ttaacagata aaaagaaaaa caatgccaaa agaaaggcaa taaatcttaa
    21961 gccccattta ctttctagca tattatttca cacctttctt ttgaaagcgt gtgccaaacc
    22021 aatgttcagc aagcaactct tcaaaaattt cgtttgaaat gtctcggcgt aattttccat
    22081 caaatgttac cgaaatatca ccagtttctt cagatacaat aacggtaaat gcatcagata
    22141 cttctgaaat accaaccgct gctctatgtc ttgtacccaa acttttagat atcttaggac
    22201 tatcagacaa tggcaaataa cttgctgctg ctgcaatctt cgtgccttga ataatcattg
    22261 caccatcatg taaaggtgta ttaggtataa agacattaat taaaagttct tgtgaaatat
    22321 ttgaatccat tgcaatacct gtttcaatat aatcttgaag acctgtttct ttttcaaaga
    22381 caattaatgc acctatacgt cttttagcca tatattgcac agcctttgaa accgattgaa
    22441 tcaatttctc ttcatcttta ctatacgtat tagaagtata gcgttttaaa aagctacctc
    22501 taccaagttg ttctaacgca cgtctaattt ctggttggaa tattactatt aaagctaata
    22561 ccccccattg aataacgata tcgaataatt tagatgttgc agtcaagttc aatatcatac
    22621 ttatctgctg accaataaca attactaata tccctttaag taattgtatc gctttagttc
    22681 ccttaaagac cgtgatgaga agataaagta cataccaaac tatcagtaaa tcaaggatac
    22741 tcgttacaat ttttaacgta ctgaggtttt gaaaaaagtt ggaaaaatcc ataacatctc
    22801 ctccgggtaa tatttttcca taatacccat tataccaatc atttacatca ctgtcatacg
    22861 aacatatatt taaatagaaa aaaatttatt ttaaagatta taactactct taatcatttt
    22921 agtgaattaa aaaaagtagt gcaaaaagca aaatatactt tatacactac aaatcattta
    22981 tttataataa agtttcacca aaaaatgttc caactaacga aaccgattgt tcagcagtat
    23041 ggttgttatg atcaataagt ggattcactt caactaagtc cattgaagta actgactgag
    23101 actgatgtaa taattccagc gcaaaatgac tttctctata tgtaagtccg cctaagactc
    23161 tagtacctgt gcctggcgtt tcaagtggat ctaaggcatc aacatctaaa gataaatgaa
    23221 cgccatcaac attgcgtgac ttcaaatatt ctattgtatt ttcaattact tcctttatcc
    23281 ccaatttatc aatatctgac atagtaaatg ttttaatatt atgatctttg ataaattgtc
    23341 tttcaccttt atctaaatct ctcataccaa ttagtacgat gttttctggc ttgattacat
    23401 tactatttaa ttctaaaagt tctttaggac cttcacctgt caaaatcctt agaggcatac
    23461 catgaatatt tccacttggt gactcttcag gtatatttaa atcaccatgt gcatcatacc
    23521 aaataacacc taaattatta taatgtttac ttattgctga tactgaacct atcgcaatag
    23581 aatgatctcc accaagaact agaggaaatc tgttattttc aattgatgct gatacttctt
    23641 tatttaattt ttgagtaaca tctataattt catcgtagtt tcttaatcca ttttgttcac
    23701 tttgaaattt ttcaatgtct acagctgata ccttaatatc ccctttgtca ttaacatcaa
    23761 ggtctaattg ctttagtctt ggtattaaac ctgcatatct aatcgcagtt ggtcctaaat
    23821 caacacctaa ttttctttgt ccaaatgttg acggtacacc aataatatca attccttttt
    23881 tctttgtcat aagcgtcccc ctttgttata cataatttca gaataatgta tacgcttact
    23941 ataatcaagt aatagccata acataacgcc ttagcatcct aattctcaaa aaataaaaaa
    24001 agcacttccc aagaatggaa agtgcaagta gtgagccata gaggattcga acctctgacc
    24061 ctctgattaa aagtcagatg ctctaccaac tgagctaatg gctctaatgg ctgggctagc
    24121 tggattcgaa ccaacgagtg acggagtcaa agtccgttgc cttaccgctt ggctatagcc
    24181 caatatatag atggtggagg ggggcagatt cgaactgccg aacccgaagg agcggattta
    24241 cagtccgccg cgtttagcca cttcgctacc cctccagctt attcagttaa ttaataatca
    24301 aaatggtgga gaatgacggg ttcgaaccgc cgaccctctg cttgtaaggc agatgctctc
    24361 ccagctgagc taattctcca atataatgac tcctacggga ctcgaacccg tgttaccgcc
    24421 gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccat ggctccacag gtaggactcg
    24481 aacctacgac cgatcggtta acagccgata gctctaccac tgagctactg tggattaata
    24541 ttaatgcctg gcaacgttct actctagcgg aacgtaagtt cgactaccat cgacgctaag
    24601 gagcttaact tctgtgttcg gcatgggaac aggtgtgacc tccttgctat agtcaccaga
    24661 catatgaatg tagattatac attcaaaa
//
LOCUS       DATTQF010000023        24558 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.023, whole genome shotgun sequence.
ACCESSION   DATTQF010000023 DATTQF010000000
VERSION     DATTQF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.023"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..84
                     /locus_tag="VB241_000867"
     CDS             <1..84
                     /locus_tag="VB241_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HEP1244177.1"
                     /translation="EILFFTLYLLLILIVLYYLKKQLKNKT"
     gene            complement(320..1585)
                     /gene="lysA"
                     /locus_tag="VB241_000868"
     CDS             complement(320..1585)
                     /gene="lysA"
                     /locus_tag="VB241_000868"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEP1244178.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCLQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAIKAINKVQQSKYLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQDIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(1575..2660)
                     /locus_tag="VB241_000869"
     CDS             complement(1575..2660)
                     /locus_tag="VB241_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP1244179.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHEIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(2665..3816)
                     /locus_tag="VB241_000870"
     CDS             complement(2665..3816)
                     /locus_tag="VB241_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1244180.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTTLM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSYLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(3959..4678)
                     /gene="dapD"
                     /locus_tag="VB241_000871"
     CDS             complement(3959..4678)
                     /gene="dapD"
                     /locus_tag="VB241_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEP1244181.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFGDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(4705..5427)
                     /locus_tag="VB241_000872"
     CDS             complement(4705..5427)
                     /locus_tag="VB241_000872"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEP1244182.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEAFHLPLVVATTGEKEKLLNKLDELSKNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(5424..6311)
                     /locus_tag="VB241_000873"
     CDS             complement(5424..6311)
                     /locus_tag="VB241_000873"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEP1244183.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKELILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(6313..7302)
                     /locus_tag="VB241_000874"
     CDS             complement(6313..7302)
                     /locus_tag="VB241_000874"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEP1244184.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKGGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(7366..8571)
                     /locus_tag="VB241_000875"
     CDS             complement(7366..8571)
                     /locus_tag="VB241_000875"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEP1244185.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKTPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINDHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILERKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILKTLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            complement(9428..11029)
                     /locus_tag="VB241_000876"
     CDS             complement(9428..11029)
                     /locus_tag="VB241_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1244186.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            11176..12078
                     /gene="cvfB"
                     /locus_tag="VB241_000877"
     CDS             11176..12078
                     /gene="cvfB"
                     /locus_tag="VB241_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEP1244187.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKH
                     KIINIETGKITLTKKGWSRIESKE"
     gene            12389..13372
                     /locus_tag="VB241_000878"
     CDS             12389..13372
                     /locus_tag="VB241_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEP1244188.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEGGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            13563..14489
                     /gene="pstC"
                     /locus_tag="VB241_000879"
     CDS             13563..14489
                     /gene="pstC"
                     /locus_tag="VB241_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP1244189.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            14491..15408
                     /gene="pstA"
                     /locus_tag="VB241_000880"
     CDS             14491..15408
                     /gene="pstA"
                     /locus_tag="VB241_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP1244190.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKYSKKF"
     gene            15455..16306
                     /locus_tag="VB241_000881"
     CDS             15455..16306
                     /locus_tag="VB241_000881"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP1244191.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPTVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            16313..16954
                     /gene="phoU"
                     /locus_tag="VB241_000882"
     CDS             16313..16954
                     /gene="phoU"
                     /locus_tag="VB241_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP1244192.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYNINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(17094..18908)
                     /gene="pepF"
                     /locus_tag="VB241_000883"
     CDS             complement(17094..18908)
                     /gene="pepF"
                     /locus_tag="VB241_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP1244193.1"
                     /translation="MSQGLPLREDVPVSETWDLTDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEKSCPYQHYIKQLIKQKPFQLSASVEQLLAILS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTLNEMLMANYLFNKSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYRKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            19113..19457
                     /locus_tag="VB241_000884"
     CDS             19113..19457
                     /locus_tag="VB241_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244194.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            19405..19590
                     /locus_tag="VB241_000885"
     CDS             19405..19590
                     /locus_tag="VB241_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244195.1"
                     /translation="MKNTAMRALNFAVKIRNNIRQLYAQNHQLLSYTAVTIISKVVEY
                     SAIVVFVLTFLYFTWLT"
     gene            19761..20747
                     /locus_tag="VB241_000886"
     CDS             19761..20747
                     /locus_tag="VB241_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244196.1"
                     /translation="MFIIKSILYRLIQMIVVLFVISTLAFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKLSDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVLTLSLGMCAYIIRLVRSNLLILLKSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            20740..21570
                     /locus_tag="VB241_000887"
     CDS             20740..21570
                     /locus_tag="VB241_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244197.1"
                     /translation="MHKIFSKNNLIFFVFGAFIFVMIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPIRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYNESH
                     "
     gene            21557..22330
                     /locus_tag="VB241_000888"
     CDS             21557..22330
                     /locus_tag="VB241_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244198.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIYNQQINALIGESGAGKS
                     LIAKALLEYLPFDLTCTYDSYQFDGENVSRLSQYYGHTIGYIYQNYAESFNDHTKLGK
                     QLTAIYRKHYKSCKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LKPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            22323..23024
                     /locus_tag="VB241_000889"
     CDS             22323..23024
                     /locus_tag="VB241_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244199.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIEISLKEALYYYRGLTDNTA
                     QDQLLIQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFTIEELFNV
                     DRHPYTKELVQAFSY"
     gene            23147..23605
                     /locus_tag="VB241_000890"
     CDS             23147..23605
                     /locus_tag="VB241_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEP1244200.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHETEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPLADGRRVICKHMIALLFTASPEAANKHIIMLNEVEEDY
                     HLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEDEFY"
     gene            complement(23653..24421)
                     /locus_tag="VB241_000891"
                     /pseudo
     CDS             complement(23653..24421)
                     /locus_tag="VB241_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
BASE COUNT         8093 a         4089 c         4000 g         8376 t
ORIGIN      
        1 gaaattttat tttttacact ttatttgtta ttaatcctta tcgttttata ttatttgaaa
       61 aagcaattaa agaataaaac ctaatttcat gatgcttgtt taaagtgtgg tatgtatatg
      121 atgctttaaa ttagttgaat agagctaact aaattaaagg attaacgaca aaaaataaac
      181 actttctaac taaaaaagtg ctgttcttcc ttttttagag tatatgtcca agcatgttat
      241 ctacagccat ctataatgct taaaaattcc ccgagtcaca gtataatttt ctgttcttcg
      301 gggattactt tatttatttt tattttgtat cattaataat aagttgtctt aacgattgtc
      361 gcttaataac ttcacgtgct ttgccatctt ttaaaaaaaa cacagaaggc ttttgcattt
      421 gattgtaatt ggatgccata gagtaatgat atgcaccagt tgataatata gcaagatagt
      481 ctccacgttt gactgatgaa ggtaatttag cgtctttaat aatgatatca ccagactcac
      541 ataattttcc agctatagtt acactgtcat ctgcttcttc atttctatta acaagcaatg
      601 cttgatactt tgcgtcataa agtgcagttc tgatatgatc actcataccg ccatcgattg
      661 aaacatattt atttatctct ggaatttctt taatggttcc aacttcatat aaagtaacgc
      721 cagcttcacc tacaatagat cgtcctggtt caatacctat ttctggtgca tctataccta
      781 gaactttaat ttcggatttt attgcgtctg taatatcttt aataccactt tcgataggga
      841 aactttcatc accttcaaca tatttaatac caaagccacc accaaggttt aataattcaa
      901 cttgaatgtc ttgctcttta agccaacgta aaacaatttt agcagtttca ataaatgctt
      961 ctgtaccttc aatctgtgaa ccaatatgac aatgtacacc ttttaattta agatacttag
     1021 attgttgcac tttgttaatt gcttttatag ctaagccata ttgaattgat aatccaaact
     1081 tactatcttc ttgaccagtt tgaataaatt cgtgtgtatg tgcttcaaca cctggattaa
     1141 ctcgtaatac aacttgaacc gtatcattag catagcggtc tattaattca atttcttcta
     1201 atgaatctat aacaaaataa ccgatgttat tttctaaagc atacctaatt tcatgtttcg
     1261 ttttattgtt accatggaaa tggatgcgac ttggttcaaa tcccgcttct aatgccgtat
     1321 ataattcacc ttcagaaaca acatctaact gtaaatcttc ctcagccacc agtttgacca
     1381 tttgaagaca agtaaatgct ttagaggcgt atgaaatatt gtattttaat ccactatctt
     1441 taaatgcgcg atggtaacgg cgcatctgtt ctctaatttg tagttcatca taaacaatgg
     1501 taggtgtacc aaagctttgc gcaatcgttt ttaaactaat accatccatt gttaattcgc
     1561 cattttgatt atatttaaca gtcatttgaa atgtactcct tttttaaaga atcatgattc
     1621 atagcactta attgttcaga atttgcacca acacctttaa aggtatattc atcgatgcgg
     1681 atatcattat tataaagaat aacttcatct tgtgcatgta cattgccatc tacttcaaca
     1741 aacatatggc tcatcattaa tgcacgtatc gggtagcgtt taccattgat aagtgcttca
     1801 tgtttagcac gagttcgtaa aattccatcg ccataaccga tatcaactac agctaatttt
     1861 gtattgtttt tagtcacttc aaaggcaaag ctataaccgc aataatcacc agcttgtact
     1921 tcgcgcactt gaataacatg tgcttttaca gtcaatgact gaactatttc atgttgattc
     1981 agtgaactat atggtcttga accgtataac gcaataccta cacgcgcatg tgtatggtgg
     2041 ggtagtaata tttgtccttc ccgataaaaa ctcgcactat tttgagcatg gattaggtcg
     2101 aactgataac cttcagataa aagtgcttca acaatttcca tccattgtga acgttcaaca
     2161 ttataatctg acacatcgaa ttcatcagca tatccaaaat gggtccataa accactaata
     2221 atcatttttg cattttgatt atgatggtga tctttcaata cttctttaat ttcgtttaaa
     2281 tctttaaatc cagaccgatg taataaattt tcaaattcta agtgaacatg aatacctgct
     2341 aaatcatttt tatggttata gtaatatgtc aacgacggca aagtcatgtg tatttgatgt
     2401 tcacggacta aatcaaactc gtaaactgca ttcattaaaa agattgttgc atctggagca
     2461 agttgtctaa tttgaattgc ttctcgtagt gatgttgtgc taaatgtatc tatacctgca
     2521 tggataaact gagttacagc aaattctagg tcatagtgat atgcattatt tttaacaact
     2581 gccattaatg gctgattgtt tttgactgtg attgcatttt gtaaaaatat tttcttattt
     2641 acagaccatg ttgctgtcaa tgtattacac ctctttgtaa ttatttaata aattttcgta
     2701 aaaattaacc acgtttatta acactttttc atcaaaattt aaatatgatg tgtgcaaacc
     2761 agttacaaaa cctttatctt catttcgtgt tcctataaaa acaaagtaag ctggagctag
     2821 ttgttgacca taaaaactaa aatcttcccc aaataagaat ggcgttggtt tgtcatagac
     2881 atttaaatca gcttttatta aggcgtcctc tatttgagta cgtaatttcg gactattgat
     2941 tgtaggggga taaccttctg caaatttaac ttcacagtct acattaaaca gaagcttgac
     3001 actttctgct atcttgtgca tttgattttt aacgattgtt aaatcatcaa tatcatatgt
     3061 acgaattgta ccttctaaat aaccattact tggtacagtg ttaatcgctt caccagcttt
     3121 aaaatgacca atatgaacaa tatttcgttt taaaccgtta aggtgaaatt gttgaatttg
     3181 tgatatttga gttaatacat gttgtaatgc ttcaccacaa gaatgacctt gttctttatc
     3241 agcaacatga cttgacaggc ctgttaagaa aaagcgatac tctgttgcgc tggccgtaat
     3301 ttcttcatct cttatcactg caatgccttc atcagcaaat gggttaacat gaataccaaa
     3361 taccgcttca attggatact tatcaaaggc accggctttt attaatcgat ttgcaccgcc
     3421 accagtttct tctgcaggtt ggaaaatgaa aacgacattt tgcggtaatt gccccgcatc
     3481 ttgcatgtct ttgcaacgtt gtacaaaaag cattaatgta gttgtatgac catcatgtcc
     3541 acaagcatgc atcacatgat cagattgact gcgataaggt acatcatttt cctccaaaat
     3601 aggtaatgca tcaatatcag ctctatacgc tatcgtatgt gagccattac cttctaagta
     3661 tgcaatgacg ccagtttcca atgggcaatc gtatttaata tttaaactat ctaaaaacgc
     3721 tttaatataa gcagttgttt caaattcatg taagcttaat tcaggatgtt gatgtaaatg
     3781 acggcgatgt ttcgtaacaa attctaattc attcataatt atcaatcctt tgtgttaaat
     3841 tactatataa atagtgtaac gtatttcgaa atttgtgatc ataagtttat ttaatgataa
     3901 acaataaggt tgagacataa tcgtatctca accttgaaat tattatacgt tgacgtcact
     3961 agtcattcag ttttcttaat gctgctacaa tctctttttt agtatcttgt acttcagaag
     4021 cttgcttaat cactttcgca ggtgtaccag caacaactgc accagctggt acatcttgag
     4081 tcacaatcgc gccagctgca acaatagcac ctttaccaac acgtacacct tctaaaataa
     4141 ctgcatttgc gccgattaat acatcatcct cgattataac tggtgaagca ctagggggtt
     4201 caatcacacc tgctaatact gcgccagccc ctacatgtac atttttacca gttgtagcac
     4261 gaccaccgag agtagcattc atatcaatca tcgtaccttc gccaacgact gcgccaatat
     4321 taattgttgc acccatcata acgacagcac catcttcaat aatagcttgt tctctaataa
     4381 atgcacctgg ttcaatacgt gcattagtat ttgttaagtc ttttaatgga atggcagaat
     4441 tgcgacgatc catttcaatt tctatatctc cgaattgact accatatgct tcgtaaaaag
     4501 gtttccaatc atctgcttca caaaagatta ctttagattg ttctgaacca aatactttaa
     4561 aactttctgg atatgtgatg ccttcaaaat tgccatttaa atatacttta attggtgtag
     4621 attttttagc atcacttata tattgaataa tttcttcagc tgttaaatgt tgtaccataa
     4681 aataatcgat ctcctttaat atgtttatag gttgtcaaac gtataaaagc cgtttggttt
     4741 attaactaag cgttctgctg cttgtattgc accattcgca aaaatatctt ttgattgtgc
     4801 acgatgcgtg atttgaatcg tttcatcagt gccggcaaat agaacttcat gttcaccgac
     4861 aatcgtacct ccacgaatag aatgaatacc aatatcttgt ggctggcgtt tttcatttaa
     4921 ttcatgtcta tcataaacag gagttacatt ttctttcaaa gatacaatca cgtcatataa
     4981 tttttctaac gtaccgcttg gtgcatctac ttttttatta tgatgtgcct cagtcaattc
     5041 gatgtcgaaa tcatcaagta ggggaacagc agctgctaaa attttagtca gtgcatgaac
     5101 gccataactc atgttcgcgc tgaaaaacac aggcatattt ttactcaatt catctaactt
     5161 attaagtagt ttttctttct cgccagttgt tgccacgact aatggcaaat gaaacgcttc
     5221 atctaataaa gggaaaagca gatttggatt tgaaaaatct attgcaacat ctgcatcttt
     5281 aacatcagca atatgttggt attgttgata tggcgttgtt gctttcggtg tattttcaat
     5341 gaccccaacg atttcatgtc ctttttcttc tgctaatcta gcaacgcgct gattcattgc
     5401 accatagcca attagtaata ttttcactca ttttcacccg ctttaaatgt gtcatatgct
     5461 tcacgaagca ctttagtatc tgtatcttct aggctaacca atggtaaacg taattcatag
     5521 tttccaaatc ctaaataact tgttaaagct ttaataggaa ttgggttaat atcaactgat
     5581 agggctgata acagtgtgcc gattggttta aattgatctt gaatatctaa tccactttgt
     5641 tgagcatcgt ataacgcttg aaattcttta ggaatgacat tggcaataac agagataacc
     5701 ccttgaccgc cacgttgata gtattcgacg acgttgtcat cattgccact atataatgca
     5761 aatgaatttg tatcaattcg ctttttcact tcttctaaat actcaaaatc attcgtagca
     5821 tcttttaaag caactatata aggatgttga cttaatattt ctacagtttc tggttcaatt
     5881 gtcatgttcg ttcttgaagg aacattgtac agcacgactg gtaatttcac agcatctgca
     5941 atcgcttcaa aatgttttac taaaccacgt tgattcgttt tgttgtagta gggtgtaatt
     6001 aacataattg catcagcccc taaggcttta gcttggattg aagcttggat tgacttttca
     6061 gtatcattag ttccagttcc agctatgaca ggaacacgtt tatctacaag atcaataact
     6121 gtttttagaa tgagttcttt ttcatctgtt gttaaagtag ggctctcagc agtagttcca
     6181 ttaacgatga ttgcttgggc attattttct agtaaaaaat taacgtgtgc tttcaaagct
     6241 tcaagattaa ctttgttatt tgtaaaaggg gttgtaagtg caacgccaac accctcaaat
     6301 aaatgtgtca ttttaattcg ctccttttaa acgcataact tgttccaata cttgtacagc
     6361 atttaatgca gcacctttta ataaattgtc tgatgtacac catacatgga aagtattttc
     6421 taatgaatca tctctacgta tacggccaac aaatacttca tctttattag tagaattgat
     6481 tgccattgga tattcattgt tctctggatt gtctactaaa acaacgcgat catcttgatc
     6541 aaataatgct ttaatatctt ctgcagtcgc ttctttttca agtgttacat caatttcaac
     6601 actatgacta tcttgaacag gaacacgtgc acatgttgct gttactttta agtctggcgc
     6661 atttaaaatt tttctcgtct catcaatcat tttttgttct tcttttgtat atccgttttc
     6721 taaaaacaca tcaatatgcg gtaacacatt attataaatt ggatgtggat atgcttctgg
     6781 tgctttacca tttacacctt cagctaaatc tttcttacct ttcatccctg aacctgatac
     6841 agcttgatat gttgtatatg ccactcgttt taaaccataa gcatcttgca atacttttaa
     6901 tggcacaaca gactgaatcg tagagcagtt tggattggca atgatacctc ttgtaaaagt
     6961 aggttcattg acttccggaa cgattaaatc aatatcttct gccatacgcc attgacttga
     7021 attgtctata acgattgcac cacctttttc aaagagtggg gcaaagtgtt cgcttgtacc
     7081 gccaccagca ctcattaata cataatcgaa atgttcactt gcacgagcat cagttaattc
     7141 ttgaactgta tatgtttttc cttgaaattc aacttctttc cctgcagaac gtgctgatga
     7201 aaataatact aattcatcga aaggaatatt tttacgattt aatgtctcca acatttttgt
     7261 acctactaat cctgttgcac ccacaactgc taattttgtc ataacttgtc actccatttt
     7321 ataataattt ccaattttta gaatatttta acaatcattt taccattaaa tgttaaatgc
     7381 gtcatatagt ttttccaccg cttgttgccc attaaaatca tcaatgacgt atgaaatact
     7441 tatttcagat gttgttgttt ggtagaaagg tatattattt tcaattaatg tcaaaaatgc
     7501 ttttgatgcc acacctgaca tatcacgcat gcctgagcca attaatgaaa ttttgacata
     7561 atgctcattg attttataag ctaatgcttc atattgattc tttaatgttt taagaatcat
     7621 agaaatttga tgaaaatcac tatctttaat cgtgaaggat agttgtagtc catccaagtt
     7681 gacgatttgt gaaatcatat caacatttac agcaccttct tcaagttccg taaatagttg
     7741 ggtaagtagc tgattgtcag gtaggggata actaattgtt acatgcatca tatgtttatc
     7801 caaagccaca ccagtaactg cttttctctc taatatttct tcatttgaca taatccatgt
     7861 tcctttcacg ttcgataaag tttttcctaa atataaaggg atattatagt ttttagctaa
     7921 ttcaacactt cttgtttcaa gtacaccagc acctaaagcg ctcatttcca tcatttcttc
     7981 atatgagacg atgtctagtc gtttagcttt tggtaaaagt cttgggtcag tggcatacac
     8041 accatcaacg tcggtataaa tttcacaagg tatttgatta ctaacagcaa gtgccacagc
     8101 ggtcgtatca gaaccacctc tgcctaaagt tgttaattcc tgatggtcat tgatgccttg
     8161 aaatccagca actactaaaa tatcgttttc ttgaaaggct tgttcaaatg tttgaggatt
     8221 aatttgagca attttacttt ttaaatgatg gccaatggtt ttaatacccg cttgatagcc
     8281 agtcattgct tttgcattca caccgatatc atttaatacc attgataaat aagatacagt
     8341 ttgttgctct ccggttgtca ataataatgc cagttcttgt tgttttggtg ttttagtcaa
     8401 tgtcgataca ttcgtcatta attgatctgt tgtgttaccc atagcactta caacgacaat
     8461 taattgttca tcttgattga cccgctcctt taacatttca gcgatccttt ttatttttgt
     8521 aaaatcactg acggatgatc cgccaaattt caacacgctt cttgttacca tataatcctc
     8581 ctaattgtta ggaagggcac acaataataa aaaaagaaca agttcggaat gcgaacttgc
     8641 tcttaatcta tatacaagtg atgcactcca ttatttttaa ataatgacaa actctcagct
     8701 cttaaccaaa aagtccaaca aattataact gctattataa tcgcttcggc atcgcaccct
     8761 ttcaaattta gctgttagca gacagtaatc taaactttac tcatgattga tgcgcctcat
     8821 caaaatatta ttaaattggt atgtgttcat tatacatatg acaaatatga atgtaaaccg
     8881 ataatttaga ttttttggaa taacctgaaa attcaagtta tagcgttgct tatattataa
     8941 aaggtggtga gaatgaaact tttttgaaaa ataaaatccg aaatacttat agcgtactca
     9001 atatgcacat taaataaatg tactctttta atgcgttgat aactgtattt gtaatttaga
     9061 gaaggggtgt tcactgtgct gatgtgctaa aaaataaaaa agacacctcg atgctataaa
     9121 tattagcatc gagacgtcca gttaatgtct attaaattga atatagtctc ggacatgaat
     9181 caatgcccta ggccctgcaa tgttatattg acagtagttg actgaatgaa aatgactttg
     9241 tacctagctt ttttcaatac ttgtcagcgt ctcacataga gaaattggat tcctaatttc
     9301 tacaaacaat acgagttggg gaataagtcc caatatagaa ggtgacagta atgtcaactt
     9361 acaaaaatgt gcaagttggc cgggcctcaa cacagagatt ttcgaaaaga aattctacaa
     9421 attatattta ttttaaaacg ccgatttctt gcaagtattc ttcatatgga atttcttttg
     9481 aaacgccgcc ttgtttattt aaatcgataa cacggtttgc aatcgtgttg ataaattcaa
     9541 agtcataaga agtaaagatg atagaacctt taaatgattt aaggccatca ttgacagcag
     9601 taatactttc taagtctaag tggttagtag gttcgtcaag taaaagtaca ttcgcacttg
     9661 ataacatcat tttacttagc atacaacgta ctttttctcc acctgaaagc acactagctt
     9721 ttttcttaac ttcttcacca ctaaataaca tacgacctaa gaaaccacgt aaaaatgttt
     9781 ctgtttgttc atcttcagga gcatattgtc ttaaccaatc aacgagattc atatttacac
     9841 cctcaaagaa ctctgagtta tctttaggga agtaacttaa cgatgtagta acaccccatt
     9901 taaatgaacc ttcgtctggt tccatttcgc cagctaatat tttaagtaat gttgtttttg
     9961 caatttcact atctccaatt aaaatcgctt tatcatttgg attcattgtg aaggatacat
    10021 tatctaatac tttttcgccg tcaattgttt tagaaagatt ttgaacgatt aataagtcgt
    10081 taccaatctc acgctcaggc gtgaatttaa cgaaaggata tcttcttgat gatggttgaa
    10141 tatcatctaa ttcaattttc tcaagttgtt ttttacgact tgttgcttgt ttagatttag
    10201 aagcgttagc tgagaaacga gcaataaagt cctgtaactc tttcattttt tcttcttttt
    10261 tcttgttttg ttcttgagcc atcttttgag ctaactgact agattgatac caaaaatcat
    10321 agttaccaac ataaacttta attttaccaa agtctaaatc agcgatatga gtacatacat
    10381 tatttaagaa atgacggtca tgtgatacta cgataacagt attatcaaag ttaattaaga
    10441 aatcttctaa ccaactgatt gctggaatat cgagaccgtt agtaggctca tccagtaata
    10501 gtacgtctgg ttcaccgaat aaactttgcg ctaataatac tttaattttt tggttgtttt
    10561 ctaattcagc cattttttta tcgtgtaaag ttggatcgat acctaaaccg gataaaaggt
    10621 tagctgcatc agcttcagca ttccaaccat tcatttctgc aaattcacct tcaagttcag
    10681 cagcacggat accatcttca tcactgaaat ctggcttcat atagatttca tctttttctt
    10741 tcataacctc ataaagacgt tcgtgacctt taattacaac atcaagcacg cgttcatctt
    10801 cataagcata gtggtcctgt tttaaaacag ctagacgttc atttttacct aatgaaacat
    10861 gtcctgtttg agaatctaat tcaccagata atatttttaa gaatgttgat ttacctgcac
    10921 cattcgcacc aattaatcca taacaattac cttctgtaaa tttaatattt acatcttcaa
    10981 atagtttacg atctccaaaa cgtaaactca catcagttac ttgtaacatg cattttctcc
    11041 tttttttcat tcgatattct aacggaagaa ttatatcata ttatcgtcac agtttcgacc
    11101 tcatataagt tgtaatgata gaatgactca cacatgttat aataataaag aatacaagaa
    11161 tcgaaggaga ataacatggc attagacaaa gatatagtag gttctataga attccttgaa
    11221 gtagtagggt tacaaggctc aacttacctt ttaaaaggac caaacggtga aaacgtaaag
    11281 ttaaaccaat cagaaatgaa cgatgatgat gaattagaag taggtgaaga atatagtttc
    11341 ttcatttatc caaaccgttc aggtgaatta tttgcaactc aaaatatgcc tgatattacg
    11401 aaagataaat atgattttgc taaagtactt aaaacggatc gcgatggggc acgtatagat
    11461 gttggattac cccgtgaagt gttagtacca tgggaagatt taccaaaagt gaaatcacta
    11521 tggccacaac ctggagatca tttgctagtt acattacgaa ttgaccgtga gaatcatatg
    11581 tatggacgtt tagcgagtga atctgttgta gaaaatatgt ttacacctgt acacgacgat
    11641 aatttaaaaa acgaagtcat tgaagccaaa ccataccgcg tattacgaat tggtagcttc
    11701 ttattaagcg aatcaggtta caaaattttt gtacatgaat cagaacgtaa agctgaaccg
    11761 agactaggtg aatctgttca agttagaatt atcggtcata atgataaagg tgagttaaat
    11821 ggttcatttt taccacttgc acatgaacgt ttagacgatg acggtcaagt catctttgat
    11881 ttactagttg aatatgatgg tgaattacca ttctgggaca aatcaagccc tgaagcgatt
    11941 aaagaagtat tcaatatgag taaaggttca ttcaaacgtg caatcggtca cttatataaa
    12001 cataagatca ttaatataga aactggtaaa atcactttaa ctaaaaaagg ttggagtcga
    12061 attgagtcaa aagaataatc atttttacac gtatcgtagg atgcgtgttt ttttattcaa
    12121 tattaaatcg gacagatgaa gtagattttt aatttcatct gtccattttt tgtgttcata
    12181 tttaaatttg gaaaatttat caggaaacat ttcaatttta ctgatgaagc aatatttttc
    12241 agattaacaa aaaataatat ttacattttc ttaacaaaca tttatgtaac atttacagtt
    12301 tctaaagatg aggttaataa ttcgaggtta agataaagat gtaatcaata caaatactat
    12361 ttgttgttca tacagggagg atatttcaat gaaaaaatgg caatttgttg gtactacagc
    12421 tttaggtgca acactattat taggtgcttg tggtggcggt aatggtggca gtggtaatag
    12481 tgatttaaaa ggggaagcta aaggggatgg ctcatcaaca gtagcaccaa ttgtggagaa
    12541 attaaatgaa aaatgggctc aagatcactc ggatgctaaa atctcagcag gacaagctgg
    12601 tacaggtgct ggtttccaaa aattcattgc aggagatatc gacttcgctg atgcttctag
    12661 accaattaaa gatgaagaga agcaaaaatt acaagataag aatatcaaat acaaagaatt
    12721 caaaattgcg caagatggtg taacggttgc tgtaaataaa gaaaatgatt ttgtagatga
    12781 attagacaaa cagcaattaa aagcaattta ttctggaaaa gctaaaacat ggaaagatgt
    12841 taatagtaaa tggccagata aaaaaataaa tgctgtatca ccaaactcaa gtcatggtac
    12901 ttatgacttc tttgaaaatg aagtaatgaa taaagaagat attaaagcag aaaaaaatgc
    12961 tgatacaaat gctatcgttt cttctgtaac gaaaaacaaa gagggaatcg gatactttgg
    13021 atataacttc tacgtacaaa ataaagataa attaaaagaa gttaaaatca aagatgaaaa
    13081 tggtaaagca acagagccta cgaaaaaaac aattcaagat aactcttatg cattaagtag
    13141 accattattc atttatgtaa atgaaaaagc attgaaagat aataaagtaa tgtcagaatt
    13201 tatcaaattc gtcttagaag ataaaggtaa agcagctgaa gaaggtggat atgtagcagc
    13261 accagagaaa acatacaaat cacaattaga tgatttaaaa gcatttattg ataaaaatca
    13321 aaaatcagac gacaagaaat ctgatgataa aaagtctgaa gacaagaaat aataagacgc
    13381 aatttcaaat gtgtcttgaa acatgatttt gatggtgaat cattatttag agtgcaaagc
    13441 ttgatttatc gagacgctga ttttgacatt cagttagtct acaagcttat caacttaaaa
    13501 tagtggttca tcattatttt acaaatctaa ttattttggg agtaatagaa agaggtttga
    13561 ttatgacttc atctactaat gttaaagctt taattgaaaa aaataataat aaaaaaggga
    13621 agcataatga caaaattata ccagttattt tagctgcaat ttcagcgatt tccattttaa
    13681 caacactagg tatattaatc acattgcttt tagaaaccat cacttttttc accagaattc
    13741 caataactga atttctattt tctactactt ggaatcctac cggctcagac cctaagtttg
    13801 gtatctgggc attgataata gggactttaa aaatcacagt tattgcgact atatttgcag
    13861 ttccagtcgg gcttggtgca gcaatttatt taagtgaata tgcaagtgac cgtgcacgac
    13921 gcataataaa accgatatta gaaattttag caggaatacc aacaattgtg tttggtttct
    13981 ttgcattaac ctttgttaca ccagtattaa gatctttcat acctggtctt ggagagttta
    14041 atgctataag tcccggctta gttgtaggta ttatgattgt ccctctcatc acaagtttga
    14101 gtgaggatgc aatggcatct gtaccaaata aaattcgaga aggtgcctat ggacttggag
    14161 caactaaatt agaagtagca actaaggtcg tacttcctgc agcaacatca ggtattgtag
    14221 cttcaatagt tctcgcgatt tcaagagcaa ttggagaaac gatgattgta tcattagcgg
    14281 caggtagttc accaacagct tcattaagtt taacaagttc aattcaaaca atgactggat
    14341 atattgttga gatagcgaca ggtgatgcaa catttggatc aaatatttat tacagtattt
    14401 atgctgtagg attcacatta tttatcttta ccttaatcat gaatttactt tctcaatgga
    14461 tttctaagcg ttttagggag gagtattaat atggaaacga cagataataa tagacaatca
    14521 ctcgtcgatc aacaacttgt ccaaaaacat ttatcatcca gaacggttaa aaataaagtg
    14581 ttcaaactca tatttttagc atgtacatta ttaggacttg tcgtacttat tgcgttgtta
    14641 actcaaacat tgattaaagg ggtaagtcat ttaaatttac agtttttcac taatttttct
    14701 tcttcaacac catctatggc tggcgttaaa ggcgcattaa tcggttcact ttggttaatg
    14761 ttaagtatca ttccattatc aatcatccta ggaataggta cagctatata cttagaagaa
    14821 tatgcgaaaa acaacaaatt tactcagttt gttaaaatca gtatttccaa tttagctggt
    14881 gtaccatcag ttgtatttgg gttattaggt tacactttgt tcgttggtgg tgccgggatt
    14941 gaagccttga aaatgggtaa cagtatattg gcagcagcgc taacaatgac cttactgata
    15001 ttaccaatta ttattgtttc aagtcaggaa gcaattagag ctgtacctaa ctcagtacgc
    15061 gaagcttctt acggcttagg tgctaataaa tggcaaacga taagacgtgt tgtcttacca
    15121 gcagcgttac ctggtatttt aactggattc attttgtctc tttcaagagc actgggagaa
    15181 acagcgccac ttgtgcttat cggtatacca actatattat tggcaacacc tagaagtata
    15241 ttggatcaat tttcagcatt acctatccaa atatttactt gggcgaaaat gccacaagaa
    15301 gaattccaga atgttgcatc ggcaggcatt atcgttttac tagttatctt aatcttaatg
    15361 aatggcgttg cgattatttt acgtaacaaa tatagtaaaa aattctaatt taaacaatca
    15421 atctaattta tctattaaaa agggagtatt aaatatggcg caaacacttg cacaaactaa
    15481 acaaatatct caaagtcata cgtttgatgt ctcacaaagt catcataaaa caccagatga
    15541 tacaaactca cattctgtta tatattcaac acaaaattta gacttatggt atggcgaaaa
    15601 tcatgcattg caaaatatta atttagatat ttatgaaaac caaattactg ccattatagg
    15661 tccatctggt tgtggcaaat caacatatat taaaactttg aatcgaatgg ttgagttagt
    15721 acccacagtc aaaacagctg gtaaaatact atatcgcgat caagacattt ttgatcaaaa
    15781 atattctaaa gaacaattac gtacaaatgt gggcatggtc ttccaacaac ctaatccatt
    15841 tccaaaatca atatacgata atattactta cgggccaaag attcacggta ttaaaaataa
    15901 aaaagttctt gatgaaatcg ttgagaaatc attacgtggc gctgcaattt gggatgaatt
    15961 aaaggatagg ttgcacacaa atgcatatag tttatccggt gggcaacaac agcgtgtttg
    16021 tatcgcgcgt tgtttagcaa ttgaacctga agtcatttta atggatgaac ccacatcagc
    16081 attagatcca atctcaacat taagagtaga agagttggtt caagaactaa aagaaaagta
    16141 cacaattatt atggttacac ataatatgca acaagcagct cgtgtatcag ataaaactgc
    16201 atttttctta aatggttatg tcaatgaata tgatgacact gataaaattt tctctaaccc
    16261 atcaaacaag aaaacagaag attatatttc aggaaggttt ggttgatata taatggcaat
    16321 aattagacaa cgatatcagg agcaacttga tgatttaata aaagaattac gtcggttagg
    16381 tgcaaatgtc tatgtgagta ttgaaaatgg tataaaatca ttaagtattg acgatagagg
    16441 ctttgcacga caaacagtta aaaacgataa acatatcaat caattaaatt ataatattaa
    16501 tgagcgagtt atcatgttaa ttacaaagca acagcccatt gcgagtgatt tgcgtatgat
    16561 gatttcttca ttaaaaatcg cctccgattt agaaagaata ggagataatg catcgagtat
    16621 tgccaatatt cgattgcgta cgaagattac agatgattat gtgttaaccc gtttaaagac
    16681 aatgggtaaa ctagctatgt taatgttaaa ggatttagat caagcattta aaaagaaaga
    16741 taccgtatta ataagagaaa taattgagcg tgatgaagat atcgatgact tatatagtca
    16801 tattattaac gcaacgtatc ttattgataa cgatccattt gtcgctgcac aagcccattt
    16861 agcagcaaga catctagaac gtattggtga tcatattatt aacatcgctg aaagtgttta
    16921 tttttattta acaggtacac attacgaaca ataacttaaa gttattacta taaaatccct
    16981 tacgataaat atatatttct attattcata aaccctcaaa aaaaccaaga ttctcacaat
    17041 tagtaatgtg aaaatcttgg tttatattgt tctactataa gttgtctcgc atcctagtta
    17101 tttgcttgct caatttcatc tgttaatttt tcaacttcat ccactaaatc agaaatatat
    17161 tgaattgtag atttaagtgg ctgttctgta gtaatgtcta cacccgcaat gtttgcaagt
    17221 tcgacaggtg atacactacc accttttttc aatgtttcta accaagcatc aacagctggt
    17281 tggccttcat ttttaatctt ttgagaaacg acagtcccga ttgttaagcc agcagaatac
    17341 gtatacgaat ataatcccat atagtaatga ggttgacgca tccatgttaa ttcagcaccc
    17401 tcagtcatgt ctactgcatc tccgaaaaat tgtttataaa catttagcat tatttcattt
    17461 aatgtcggcg catttaaaga ttcaccttga tctactttgc gatacacttc acgttgataa
    17521 gcagcttcta ataagtgggt aaccatatta tgataatatg ttctagataa aattgagcca
    17581 ataacccaac gcttaaatct tggattatca cttttgttaa ataaataatt ggccatcaac
    17641 atttcattca atgtagaagg ggcttcaaca aagtacattg atgcttctga ttcaagatat
    17701 ggttgatgtt tttgagctaa tgtaaaatga cctgcatgac ctaattcatg tgctaagaca
    17761 aatgcttcag ccattttacc agtccaagaa ataaacacat atgaatgtgt aaagtatgga
    17821 cttgcacaaa atgcgcctgt atctttacct ttattttgtg caaaatcaat ccatcgctga
    17881 tcgtatgctt cacgtaacat gtttgtatag tcatcaccta aaacacttaa cgcaccgaaa
    17941 atataatttt ttgagtcttc aattgaaatc tctggttcat aatcagggtc tacagaaatt
    18001 tttaagtctt caaaacgcat gttatctaat ccgtgaatac gttgtaaaag tttagcatat
    18061 ttctgcataa ctggtgccaa gtcacgcata atcatatcga tttgacggtc aaacatatca
    18121 cgcgttactt cttgactatg taataaataa tcgatgaccg attcaaaccc acgtaaatca
    18181 gcttcaattt tttcttgttg tacttgcata ttatatgtag ccgcggtagt atgctgatat
    18241 tttcgaatcc catcgctaaa cgatttgaaa cttttacgtc taaactcagg atctttatta
    18301 tcttcataat cattttcaaa cgtagcatag tcgacagggt acgttgtacc atcatgttca
    18361 aatgaatcga atgtaatatc tagcattttt gtcgtgccgt ataaatcgta aggactgttt
    18421 agcgtaggtg ataaaattgc taataattgt tctaccgacg cagataattg gaatggcttt
    18481 tgttttatta actgtttaat atagtgttga tatggacatg atttttctaa ttgttgaagt
    18541 atttcttctg gtaattcaag tatttctgac tctacaaatg ataattggct aacaatttta
    18601 ccgtatgtag tagataattt agcgctcaat acttgtgcct caatattact agtatctaca
    18661 cttaaacgta gttctgcata attacttaag cgatctaagg caattaaaat attttctaat
    18721 tcagctaaag cagtattaat ttgttcgatt gaatttaatg ttgttgcata tgtatgatga
    18781 aattgatttg cttgttgtac tagagcgtca atactttcat aatattgttg atcatcttta
    18841 aataagtctg ttaaatccca tgtttctgaa acaggaacat cttctcttaa aggtaaacct
    18901 tgagacatat aatcactcct ttggtataat acattcatta tacataattt tgtctcctaa
    18961 acgataatgt tacgattccc atgtcgcaag gaacaatgat ttttctaata gtagatagaa
    19021 tattgcttga aactgactgc tattgtttaa gtttttaaaa atttataata aaatgtatac
    19081 aaggaaagta atgaaataaa aggagaatga atatgacatt attcgatatg cctaattatt
    19141 tatggatcac aactttaata atgattttat taacaatatt ctgttgctta gttttaaata
    19201 aatggtttgt atcagcagta attacatttg ttatattagg tgtgcttgca ttttttattc
    19261 caaattttca agatataaaa tatcaaccat tattaggata cgcggcgttt ttagcgatca
    19321 tgagtttatt aattagtttt ctattatggt attttacaag aaattggcgt aaagaaagaa
    19381 aagcgcgtaa attagaaaag caaattgaaa aatacggcta tgagggcgct gaacttcgcc
    19441 gtaaagataa gaaataatat tcgacaacta tatgcacaaa atcatcaact tttaagttac
    19501 actgcagtaa caattatttc gaaagtggtg gaatacagtg ctatagttgt ttttgttttg
    19561 acttttttat attttacatg gttgacttga ttctaaatat tatattttat aatgattttg
    19621 ttaaaaaatt aaatatactt caatttcttt ttggataata ctataatgaa cgttagttct
    19681 taatatgtaa gttgcatata catgcgccat tctcatgtaa atagaaagtg ttagtaattt
    19741 acacaaagag ggggactatt atgttcataa taaagtctat tttgtataga ctgatacaaa
    19801 tgatagttgt tttatttgtt atttctacac ttgcattcat tttaatgaaa ttatcaccag
    19861 gcaatcctgt tgataaaata ttacatcttg atgtagcaca agtgtcgacg gaacaaataa
    19921 atgcaaccaa agataaattg ggattaaatg attctttatt ggtacagtgg tggcattgga
    19981 tgaatcattt attacatttt aatttaggaa aaagttttga atcaaaagaa ccggtaacac
    20041 agatattatt caactatgca cccataacac tactcatatc tttttcaaca ttagtagtgt
    20101 cattatgcat atctataccg ctcggcatta tagcagccaa acgatttcat aagttatctg
    20161 acaaagtgat tagagtgatc tcaactctat caattagttt accagcgttc tttatcggta
    20221 ttattttatt atttattgtc acaaatttaa tgaatataga tagcgttata ctaagtcagt
    20281 ttatattacc tgtactcacg ctatctttag gtatgtgtgc atacatcatt cgcttagtgc
    20341 gttctaattt attgatatta ttgaaaagta atatcgtaca agcatcaaga ttacgcggta
    20401 tgaatgaacg ctatatttta attcatgatt tactaaaacc aacaattttg ccgattatcc
    20461 cattattagg gatttcactt ggtagtctaa taggtggtac tgtagtgatt gaaaatttat
    20521 ttgatatacc tggtattggt tatctattaa tggatagtat taaatctcga gattatcctg
    20581 ttattcaagg atgcgtgtta tttattggct tcttcgttgt tattatcaat acgattgctg
    20641 atttattaac gttattactt gatccgaagc agcgtttaca attaggaaat ccgaaaaaca
    20701 aaaccaatac accattgata tcagaaagta gtgaccgtca tgcataaaat attttcaaag
    20761 aataacctta tcttttttgt attcggcgca tttatatttg tgatgattgt actacaattc
    20821 tttgtcagta gtgaaaatgc aaccaaagtc aatttatcac aaacttttga acctattagt
    20881 tggttgcatt tattaggaac tgatgattat gggagagatt tatttacccg aattattatc
    20941 ggtgcacgtt caacattgtt tgttactgtt ttaacattaa tagctatcgt tgtcataggt
    21001 gttacactag gtctatttgc cggatataaa aaggggtgga ttgaacgatt agtgttaagg
    21061 tttattgatg ttggtctaag tattccagaa tttatcatca tgattgcttt agcaagcttt
    21121 ttccaaccat cgttatggaa tttagttatt tcaattacag taataaaatg gatgaattac
    21181 acaaggttga ctagaagtat agttaatagc gaaatgaata agccttatat aaaaatggca
    21241 caattatttc atgtacctat aagaacaata ttaatacgtc atttaacacc taaaattata
    21301 ccggctatta tcgttttgat ggtcgttgat ttcggtaaaa tcattctata tataagttca
    21361 ctatcattta ttgggttagg tgcacaaccg ccaacaccag agtggggcgc tatgttgcaa
    21421 caaggtcgtg attttatttc gtctcatcca attatgttga ttgcacctgc ttcagtcatt
    21481 gctataacta ttttaatttt taatttaacc ggtgatgcgc taagagatag attgctgaaa
    21541 caacgaggtg aatataatga gtctcattga tatacaaaat ttaacaataa agaatactag
    21601 tgagaaatca cttattaaag ggattgattt gaaaatttat aatcaacaga ttaatgccct
    21661 gattggagag agcggcgctg gaaaaagttt gattgctaaa gccttacttg aatatttacc
    21721 atttgattta acctgcacgt atgattcgta ccaatttgat ggggaaaatg ttagtagatt
    21781 gagtcaatat tatggtcata caattggcta tatttatcaa aattatgcag aaagttttaa
    21841 cgaccatact aaattaggta aacagttaac tgcgatttat cgtaagcatt ataaaagttg
    21901 taaagaagag gctttgtcca aaattgacaa ggctttgtcg tgggttaatt tacaaagcaa
    21961 agatatatta aataaatata gtttccaact ttctgggggc caacttgaac gcgtatacat
    22021 agcaagcgtt ctcatgttga aacctaaatt aatcattgca gacgaaccag ttgcatcatt
    22081 ggatgctttg aacggtaatc aagtgatgga tttattacag catattgtat tagaacatgg
    22141 tcaaacatta tttattatca cacataactt aagtcatgta ttgaaatatt gtcagtacat
    22201 ttatgtttta aaagaaggtc aaatcattga acgaggtaat attaatcatt tcaagtatga
    22261 gcatttgcat ccgtatactg aacgtctaat taaatataga acacaattaa agagggatta
    22321 ctatgattga gttaaaacat gtgacttttg gttataataa aaagcagatg gtgctacaag
    22381 atatcaatat tactatacct gatggagaaa atgttggtat tttaggcgaa agtggctgtg
    22441 gtaaaagtac gctcgcttca ttggttcttg gcttatttaa acctgttaaa ggagaaattt
    22501 acttaagtga caatgctgtg ttaccgattt tccagcaccc tttaactagc tttaaccctg
    22561 attggacgat tgagatctca ttaaaagaag cgttatatta ttacagaggt ctaactgata
    22621 atactgctca ggaccaatta ttaatacaac atttatctac ttttgagttg aacgcgcaat
    22681 tattgactaa attaccaagc gaagtgagtg gcggacaatt acaaagattt aatgtcatgc
    22741 gttcgttatt agcacagcct cgcgttttaa tatgtgatga gataacttca aatttagatg
    22801 ttatagctga acaaaatgta ataaatatat taaaagcgca aacgattacg aacttaaatc
    22861 attttatcgt tatttctcat gatttatccg tgttacaacg cttagtcaat agaattatcg
    22921 ttcttaagga tggcatgata gtcgatgatt ttacaataga ggaattattt aatgttgata
    22981 gacaccctta tacaaaagaa ttagtgcaag cattttcata ttagttattt aagaatgcga
    23041 taattctaga cttgttataa aatatagata aatcaagtat attaatctag acacatatcg
    23101 attttatttt ctatatctaa aaataataat aaaaaggagt atcatcatgg gattacttga
    23161 tattgcaagt attcgttcta tagaaagagg ctttaattat tatcaaagtg aatgcgtaat
    23221 taacttaaaa tcattttcag aaacgcagca tgagactgaa gtaaagggca gtggtaacaa
    23281 agtatatcgt tgttatattg atatggaaca tcctagaaaa tccaaatgta attgtcctct
    23341 tgctgatgga agacgagtga tatgtaaaca tatgattgca ttactcttta cagctagtcc
    23401 agaagcagca aataaacata taatcatgtt aaacgaagtt gaagaagact atcacttacg
    23461 cagaaatatg tggattgatt ctcttaaaga aatgattaat gatatgagtg aagaagaact
    23521 ccgcgacgca tatttaaaca tgttaattga acatggagaa atggcagaat tatttggatt
    23581 agatgaagag gacgaatttt attaaaatag cccctatcca ttgataatga ttatcatttg
    23641 ataggggtgt ttttatttat atgattttaa gactttgcaa atagctagtg cattattatc
    23701 gatgtgtttg actttatcaa gtttcaatat tgcgtgtgtg tatgcttctg atggtcctat
    23761 aatatagcca ttactagcat gttgtaacat gacaatatca ttgtgatcat taccaaatgc
    23821 tatatattta acatctgcat caaatatata ttgtaatgca gtatatttat taatattttg
    23881 cgctgttata tcaatgttat attcatttga atggtgaatc atttccaatt cttgatggta
    23941 tttattgagc tcatctaata tagttgtaat ttgtgtcggt tctatattta ataaaataat
    24001 cttgatcggt gtgtcgatat ttgcaacatc aatacaactg gccagcttat gtggatctaa
    24061 acgctcaaaa atagcgttct cagcgtcaag ttgtgcagca taattccaat catcatcaat
    24121 aatataatct aactcatact tttgaattat ttttaagata tgatgatatg tatctgtatg
    24181 aattggtttg ataacagaaa tcattgattg ctgtgaaatc atagcaccat ttgcgccaat
    24241 taatgtgtgc tgataaaaat actgatggta aaactggcaa caaatcacga atcggacgtg
    24301 ctgatgcaaa tataagttca tgaccagcat gttgtaattg taacaatgta tcaataatag
    24361 tctggtcaat taatcggccg tcaaaacaaa gtgtaccgtc aatatcaaag acaaatttca
    24421 tcaaatcact ccaaacaata taataccatg attatagcat aagtaagtta tgtgacgata
    24481 ttagataatg attatgatgt agggtacctt ttgcgttaga cacatataac ttcctataat
    24541 atatattatg taaactag
//
LOCUS       DATTQF010000024        23493 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.024, whole genome shotgun sequence.
ACCESSION   DATTQF010000024 DATTQF010000000
VERSION     DATTQF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.024"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(88..567)
                     /gene="rlmH"
                     /locus_tag="VB241_000892"
     CDS             complement(88..567)
                     /gene="rlmH"
                     /locus_tag="VB241_000892"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP1244201.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(935..3253)
                     /gene="adsA"
                     /locus_tag="VB241_000893"
     CDS             complement(935..3253)
                     /gene="adsA"
                     /locus_tag="VB241_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HEP1244202.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSSAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHYGEEAATNVSASAQGTADDTNNKVTSNAPSNKPSTAVSTTV
                     NETRDVDTQQASTQKPTRTATFKLSNAKTASLSPRMFATNVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQSSKGSESGKDAQPIGKDKVMDPA
                     KQPAPSKVVLLPAHRGTVSSGREGSDRALEGTAVSSKSGKQLASMSAPKGSTHEKQLP
                     KTGTDQSSSPAAMFVLVAGIGLIATVRRRKAS"
     gene            complement(3480..4280)
                     /locus_tag="VB241_000894"
     CDS             complement(3480..4280)
                     /locus_tag="VB241_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1244203.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(4668..5456)
                     /locus_tag="VB241_000895"
     CDS             complement(4668..5456)
                     /locus_tag="VB241_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEP1244204.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIQLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNSPKIINH"
     gene            complement(5457..6791)
                     /locus_tag="VB241_000896"
     CDS             complement(5457..6791)
                     /locus_tag="VB241_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244205.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALATVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGANQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNPDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(6784..8610)
                     /gene="walK"
                     /locus_tag="VB241_000897"
     CDS             complement(6784..8610)
                     /gene="walK"
                     /locus_tag="VB241_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEP1244206.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSTKDTTFIRDIPKKTIFTEFDLDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8623..9324)
                     /locus_tag="VB241_000898"
     CDS             complement(8623..9324)
                     /locus_tag="VB241_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1244207.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(9930..10002)
                     /locus_tag="VB241_000899"
     tRNA            complement(9930..10002)
                     /locus_tag="VB241_000899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9967..9969),aa:Asp,seq:gtc)
     gene            complement(10010..10084)
                     /locus_tag="VB241_000900"
     tRNA            complement(10010..10084)
                     /locus_tag="VB241_000900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10049..10051),aa:Glu,seq:ttc)
     gene            complement(10514..11797)
                     /locus_tag="VB241_000901"
     CDS             complement(10514..11797)
                     /locus_tag="VB241_000901"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP1244208.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(12075..13475)
                     /gene="dnaB"
                     /locus_tag="VB241_000902"
     CDS             complement(12075..13475)
                     /gene="dnaB"
                     /locus_tag="VB241_000902"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP1244209.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(13507..13953)
                     /gene="rplI"
                     /locus_tag="VB241_000903"
     CDS             complement(13507..13953)
                     /gene="rplI"
                     /locus_tag="VB241_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP1244210.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(13950..15917)
                     /gene="gdpP"
                     /locus_tag="VB241_000904"
     CDS             complement(13950..15917)
                     /gene="gdpP"
                     /locus_tag="VB241_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HEP1244211.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(15932..16870)
                     /locus_tag="VB241_000905"
     CDS             complement(15932..16870)
                     /locus_tag="VB241_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HEP1244212.1"
                     /translation="MGVAYVFSKIQPKATMIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(17163..18131)
                     /locus_tag="VB241_000906"
     CDS             complement(17163..18131)
                     /locus_tag="VB241_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEP1244213.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYVPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNRQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKERFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(18190..18288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18493..18822)
                     /locus_tag="VB241_000907"
     CDS             complement(18493..18822)
                     /locus_tag="VB241_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEP1244214.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(18819..19514)
                     /locus_tag="VB241_000908"
     CDS             complement(18819..19514)
                     /locus_tag="VB241_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEP1244215.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWVIPCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(20158..21444)
                     /gene="serS"
                     /locus_tag="VB241_000909"
     CDS             complement(20158..21444)
                     /gene="serS"
                     /locus_tag="VB241_000909"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP1244216.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDSKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(21534..21749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21822..23336)
                     /gene="hutH"
                     /locus_tag="VB241_000910"
     CDS             complement(21822..23336)
                     /gene="hutH"
                     /locus_tag="VB241_000910"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEP1244217.1"
                     /translation="MTLYLDGETLTIEYIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQMNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANATFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITYDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         6925 a         4638 c         3571 g         8359 t
ORIGIN      
        1 acctttatga tgttatattt tatttttttg ctgtttttat caccatattg aatttatact
       61 atattaatag ttactccatt tttagtttta tttgtgatac gcctctccac gcataatctt
      121 aaatgctctg tacacttgtt caattaacac aacccgcatc atttgatgtg ggaatgtcat
      181 tttgctgaat gatagtgcgt agttactgcg ttgtaagacg tccttgtgca ggccgtttga
      241 tccgccaatg acgaatacaa agtcgctttg cccttgggtc atgcgttggt tcaattcttg
      301 ggccaatcct tcggaagata gcatctttcc ttgtatttct aatgtaatga ctgttgattg
      361 tggtttgatt ttggctagta ttcgttggcc ttctttttct tttacttgct caatttcttt
      421 gtcgctcata ttttctggtg ctttttcgtc tggaacttct atgatgtcta tcttggtgta
      481 tgggcctaaa cgtttttcat attctgctat ggcttgcttc caatatttct cttttagttt
      541 ccctacagct aaaatggtga ttttcatgtc gtttggtcct ccaaattgtt atcaactttc
      601 cagttatcca caagttatta acttgttcac actgttccct cttattatac caatattctt
      661 tgcagttttt gatatttttc tgacattcat gcccaatttt tagtgtataa cctgtgtatt
      721 tgtggataac tctgtgggca ttgtgggtat ttctctttaa attgtacaca attctggata
      781 actatcccca tttctgtgga taaatacctt gatgtcttat tacttatccc cgtttttata
      841 tcccatattt atcaacggtt atagcttttt ttcataaaaa aacactaccg tacctcttgt
      901 ttaagaaata cagtagtatt gttttcaata tattttagct agcttttcta cgtcgtacag
      961 tcgcaattaa acctatacct gctactaata caaacatcgc tgctgggctt gaactttgat
     1021 cagttccagt ttttggtaac tgcttctcat gtgtgctacc tttaggcgct gacatgctag
     1081 ccaattgttt cccgctctta cttgatacag cagttccttc caatgcgcga tcagaacctt
     1141 ctctaccact actaacagtt cctctatgcg ctggcaacaa tacaacttta cttggcgctg
     1201 gttgtttcgc tggatccatc actttgtctt taccaattgg ttgtgcatct ttaccagact
     1261 cactaccttt gctactttgt tgtcctttag ctggttgttc acttactgct ggtttaccta
     1321 ataacatacg ttgtggttct gtcgtatcat acttagctaa gttagctgtt tttaaataac
     1381 ttgctagtac ttgatctaat gaaatacctt cttctctagg gccaccgaac atactatatc
     1441 cgtcgccacc tgatgctgtg aagtcattca tcgttacgtg atatacacgt tttaaatcaa
     1501 tattttcaaa cttacctgtc tctttattta aaatttgaat agcattaatt cgtttgccag
     1561 acggtttatt catatcatag taaacacgga ttgaatcaga gatatgtagt aaaccgccat
     1621 tcgctgttaa tactgtctta ccatcttttt gtgttgttgg tgcgcctaaa ctatgttcaa
     1681 aagccgtcca gacgtctgaa ccttttacat caatttgcgc aatcgtattt ccaaatggta
     1741 atactgaaat taaatcatag cgtgtcacct taccttttgc gatagaggca cgaatacctc
     1801 caccatttgt cacggcaaag tcagtctttt tagagaaatt cttaacgcca tatgcttcca
     1861 tagcatctgc aatcgcgttt cctaaatttg tttcacgcgt tctaacgtca tctctttctc
     1921 ctttgaaatc aatcgtatta tttggaataa taacctctgc tgtttgtgct ctaaatgttt
     1981 gatcagcttg attaatttgt tcagctaatg ctttgttcgg tgttacattt tcaacgtctt
     2041 taacattaat caatgatggt ttaatatttg atacctctcc attgcggtaa ttaaatgtaa
     2101 tcttaccgat attcgcaagt gctgtacctg tttgtgctaa tgcatcattg ttataaattt
     2161 gaccattttg aagtacggta tgtgaatgac catcaatgac tgtaatacgt ttcttcaatt
     2221 gtggattttg acttaattgt ttcactaagt aatcgccacg ccatgtttct tgtgttgaag
     2281 gatcaatacc taaatgagat ataacaacaa atgtatctac gtctttataa atacgcatca
     2341 tttctgctgt cacactttgt aatggatctc taaattcaac acctttaatg ccttcaggcc
     2401 ttgttttcgt ctttgtttct ggtgtcgtta cgccaataat tccataacga ataccatttt
     2461 tcgttacaat cgttgaaggt ttaaatgcgc gttttccatc tttataaacg ttagtactta
     2521 acatcgggaa gtctaacata ccctctaact ttttcaactg gtcgtatcca aagtcaaatt
     2581 cgtggttacc gactgccata gcatcataac ctactgcatt catcgcttta gccatttctt
     2641 cacctttaga ctggtttgaa agtggtaaac cttggaaggc gtctcctgcg tctaacatta
     2701 aatcaggctt ttcttgttct tttactgttt ttaatttagc cataccgatg acacgccctt
     2761 tttcttcggc tagtcggcca tggatatcat ttgtatgtaa tattttatgt gttgttgttt
     2821 gtggtacatt ggtagcaaac attcgtggtg aaagtgatgc tgttttagca tttgataatt
     2881 tgaatgttgc tgtgcgagtt ggtttttgtg ttgaggcttg ttgtgtatct acatcgcgcg
     2941 tttcgtttac tgttgttgaa actgctgtag atggtttgtt agatggtgcg ttggatgtta
     3001 ctttgttgtt tgtatcatca gctgttccct gtgctgatgc tgatacgttg gttgccgctt
     3061 cttcaccata atgatgagcc gcttcctttg ttggcggtac ttgttgctta tctgttgttg
     3121 ctttgtctat cgttgttact gcatgtgctt tcattggtgt atgttgctca gccgcgctcg
     3181 agacttgcca taatcccatt gcactaaaaa gcaaaacgag ccatacactt gttttaagta
     3241 ataaagcttt catttcgttc cctccattaa ctgtcataca ccaatatatt atcataatcc
     3301 ttatattaaa atgattgatt ttatgtaaat tatttataaa ttacatttaa tatcgaatta
     3361 ttttttgcgt ttcaactaga accttaatct caacaacagc catttcccac caacaacata
     3421 aaaaaacacc cgataaagtc gtttcacagc agtgcggaac tttatcgggt gactctcatt
     3481 tatattgtat atattggcgt tggaatagct ttatccgtat cacatagcaa tacttctttt
     3541 tccgtatcaa tatcgtgttc gttcaatact tggccaacac tcatacgcgc caaatctttc
     3601 atgttattat cttgtgataa atgcgatagg taaatacgtt tcgtgttacc tgtaatcacg
     3661 tctgtcatcg catgacccgc atcctcatta gatacatgac ccatatcgcc taaaatacgt
     3721 tgtttcgtct tccatggata acgacacatt ctcaacatat cgacgtcatg attactttca
     3781 aaaataaacg catcgctgcc acgtatcata cctttcatac gatcagacac gtaacccgta
     3841 tccgttaaaa tcgtaaactt cttatagtta ttatggaaaa tataaaattg cggatctatc
     3901 gcatcatgtg acacgttaaa cgattcaaca tcgaaacctg caatagattt cgtttcataa
     3961 ggattaaaaa tgaatttctg atccataggg atgcgactat ctttcttttc aattgcctgc
     4021 caagtctttt cattcgcata aattggcaat tgatatttac gcgccaaaac acctaatcct
     4081 ttaatatgat caatatgttc atgggttact aaaataccat ttaaatcttg aatattacgg
     4141 tcaatttgac taaacaattc ttccattttc ttgccagtca aaccaacatc aactaataga
     4201 ctaccttttt cattttctac aaaagtggcg ttacctgtac taccacttgc taatacactc
     4261 atgcgtatca agcggctcat cctttctatt taagcagtca atctctctat atgactgcaa
     4321 tatacatcag tgcaaatata cgattttccc tacagaaact atcagtatat tagctgtttt
     4381 gtataccttt cctattctgt aactttagtt catcgaccgg tctcttataa ttaaattttg
     4441 ataatcacgt gcaatgaatg tcctaattat tataacaatg attagacaaa tactaaagtt
     4501 tttaaaatag cagttacgtt aattttacaa tgcggttcta cttaatatat gaataaatgc
     4561 gcatgttagg acgcaaaaac gtcagccata ataaaccata ccagcaaaaa gctagcaccg
     4621 cttctcacat atgatgagct tgcaatgcta gcttattacg attcatatta atgattaata
     4681 attttagggc tattatttgt cgcttcaaca tagtaagtat tcgttttatc cttaccgtca
     4741 tgcttcactt taatttccca gtttggttgt aacaattgaa cattcgtttc gttcacgact
     4801 gagtagtaac cgagtctagc attaatgact tcatcacctt ttttcaagta acgattataa
     4861 tataatgcct cgattgcttt tctcacacca atcacttgat gcttcttatc tgcaccatct
     4921 gtgggcttaa tgtcatccat cattgattgt ttataactag tcgctttatt atcttctata
     4981 ttaaagttca acatcgcttt actattattc aaaatcggga aattatcata cgtttgttcg
     5041 tattttacag aaccagaact aatctcactt aattgatatt ctgaaccttt gaacacacgt
     5101 ttgttaacat aatctttcaa atcttttaag ttattgtcac tgacctttac cgattggcta
     5161 atgtttgcat ttaataattt tccaccatct gatgtggtca aatccgaatc gcccttagct
     5221 ttagaactaa agtcttttga acgccccgta atttgttcta attgtatgcc tttaactgat
     5281 ttatttaaaa tactagccgg tactttgatt tcttcttgtt gaaaattaac ttcattgtta
     5341 ctctcaactt cattaatgtg tgagcgattg actttattaa cataaatcga tactaatacg
     5401 atgttgacaa gaataaacac gaaaatgaaa agtgtcttcg tcagtttcca gttcatttat
     5461 tcaagcctcc catcgttata aacataccat tcgccatcat attctacata ccaacgcggt
     5521 acgagttcac tgttaatctg cacttcaatg tgattatgat ccggattatc ttgcatttcg
     5581 taaccgatag cgatgtttgt tactttttca aaattaatat cactattgtt cgctaagctt
     5641 gaacgtacag actctaattt cggcaacgat ttattatcct cactatttaa cacaacatcg
     5701 gtgcgcagta acgaacgacg ataatcaaag acgccctttt caccccaagt gacttgaatt
     5761 tgattagcgc cttctttatt aaacgttgga taaccattaa ggaaacgttg atatgttagc
     5821 tcgcctgact gattattcgt actaaacaat ctgaagtctt cgtttaagaa accaccatga
     5881 ccattaataa aatcaaaggt tcctggaatc gtttcttcca ttttgcttga acttgcttca
     5941 tcttcggaca agtttttata atggtatttt tcatttttat cgttatagtt tgcgacacct
     6001 gtattattgt tgtaagttgt aacaccactc tttgaactac gaacgatggt tgaatcgtca
     6061 aatagtatag cattcatttt ctcaacacta atcgtgttaa ataccatgcg atatgttttt
     6121 aacttttcag gtttacttgg tgcaaaaaca tgcgtcgtac gatcaattgt atctttgttt
     6181 gtgatgatat ctgtgtatgg ttgcatatct tttttcactg ttgctaatgc atctaaaaaa
     6241 tgatcatttt tcgttgtagt tgttaatttt acgtaatcgt ggcgatcttt gcttatagca
     6301 taaagcacga tattatcatc agcatcatga tctatgacca aacgattgaa attgaaatga
     6361 tttggtactt tcgcattcat gttcagtact tggccaagat atgttgataa cggtaaatca
     6421 tacgtaaaat cgaataatgt aaaatcactg ctcaaatcag gaatcatcaa gttatgatca
     6481 cgacgaacat gttccacgga cttcacttct ttatttttta aaggtttcgt cagcttattt
     6541 acattagata ccgtcgcaat cgtaccttct ggatgatcat ttttcgaatg aataatctga
     6601 aatggcgtaa tagttgtatc cattttggct gtcataggtg tcgttaaagg tttcgtttca
     6661 ctcttcttac tatctgtatt gtcgacattt gcaatatcag gcgaaaagtt ccataccata
     6721 tatgtcaata cgacactcat caagacgagt agcgctaaaa tgacagattt aatatgttcc
     6781 ttattattca tcccaatcac cgtcttcaat gacttcacat ggaagtgtga taaagataga
     6841 tgtgccttga ccttctacac tgtttgccca aatacgacca ttgtgagctt ccacgatctc
     6901 tttcgaaatg gctagtccta atccagtacc acccatttta cgcgtacgtg ccttatctac
     6961 acgatagaat cggtcgaata tcttatcgac tttattgata ggaataccga tgccattatc
     7021 tttaatacga atcgtcattc gattataaag tggattttgt ttcacgtgga actcgacacg
     7081 tttatcgcct ctagaatatt tcatcgcatt tgtaatgaca ttatcaaata cttgcgtcat
     7141 tttatcaaga tcaaattctg tgaaaatcgt ctttttcgga atatctcgaa taaatgttgt
     7201 atctttcgta gacatttcat gtcgattaat aattttatta atgaacatgt taaagtcgat
     7261 aatttcttta ttgatttgat cagactcatt atccatttta gataactgta gcaagtcatt
     7321 gaccagtcga atcattcgtt ctgtttcttc acgggtaaca gataaaaatt gtggcgcaag
     7381 ttcctcatct ttccatgcac cttcttcaag tgcttcaatg taactattca tagaagttaa
     7441 aggtgtacgt aactcatgtg atacattggc aacaaattca cgacgctcac gttcaacttg
     7501 ttgttgttca gtcacgtcat gtaacacagc gatataaccc gttacaaatc ctgtttcctg
     7561 cacaatcgta ctaaagttaa cacgtgcgat tagaccttct tcttcattta aatctaataa
     7621 gaaactatca ttattctctt gaatttcttc aagtttaaat tcatcttcaa gacttaatac
     7681 acttaacatg taatatccga tgatgtcttc tttcgccata ccaagcatct taagtgccat
     7741 atcattgaca atacgaatac gtccacgacg gtctgttgca ataataccat cactcatatg
     7801 ggtgataact gagtccagtc tacgtttctc actttcagta ttagcctgcg cttcttgtac
     7861 acgtttagac aagttattaa atgctaaagc taattcgcca atttcatcat taccataaat
     7921 cttcacacgt tgcgtatagt tacctctgga catttcaacc gtctggttac gcatatcggt
     7981 gattggtttg gtaatcgttc gcgctataaa gaatcctagg atgactgtga ttaataatga
     8041 aatagctgta ccaacaatga atatttgatt tatattattt aattggttat aaacgtcatt
     8101 aatttttgat tcgatataaa tattacctat taccttttta tcgactttaa cggggatatt
     8161 atatacccag acacggtcct taccaccgcc ataatctttt aaaattaaat gatcgtttga
     8221 ttgtcctagt gatagtgctt tttggacaga actatcattc gctttttgat tgattagact
     8281 acggttagac tgcttcgtcg tcgcaataat aatttggtct ttatctataa aacgaatttc
     8341 tccaatttct tgacggttgg catactcact taataaattt tgaatatctt tttgtgcatt
     8401 tacggagccc ttttcgtcat atactttttc aatactaatt tctaattgct tagcgtactg
     8461 cgtaatattc ttcttaaaat tatcaagcag ctctttttca aggttatttg taaaatacag
     8521 cccgataatt tgcataccaa tgataatcag taatacataa acaattacaa gtttagtatg
     8581 aagggattgt agttgtttta gccacttcat tcgtttcgac ctctactcat gttgttggag
     8641 gaaatatcca acgcctctac gcgtcacaat atattcagga tgtgacggat catcttcaat
     8701 cttttcacgt aaacgacgaa tcgttacatc gactgtacgt acatcgccaa agtaatcata
     8761 gccccatact gtttgtaata aatgttcacg tgtcattact tgtcccatat gttttgacaa
     8821 ataatggaac aattcaaatt cacgatgtgt taattcaata tcttcgccac gttttttaat
     8881 agaatatgcg tctggataaa tcacaatatc tttaattgtg atttcattcg ttacatttcc
     8941 agtgtcttgt gctggttgtg agtaatgacg acgtaagttc gctttcacac gtgcgattaa
     9001 ttcacgcgta ctaaacggtt tcgttacata gtcatctgca cctagttcta aaccaagcac
     9061 tttatcaatt tctgaatctt tagcagtaag cattataatt ggcatttcgt attttttgcg
     9121 cacttcacga catacttcca taccatcacg accaggtaac atgatatcta gtaatacgat
     9181 gtctggttct tcttcataaa ttaagtcgac tgcatcatta ccatcgtatg cacagtacac
     9241 atcgtatcct tcttttttta agttaaattc taaaatatca gcaatcggtt tttcatcatc
     9301 aactacaaca acttttctag ccatttgcat aaacctcttt tcttaaatca ttaatattat
     9361 tttatcattt tttgttcttt atcgtttatg tttgtgtaaa aaatcacagg atgtcgctta
     9421 ctatttttgt tattgtgcgc attttccgtc ttataacgta tagtaaccta ttatataatt
     9481 taccatttat aagctattaa ttctattttc caatataaac ttatatatta atatttttta
     9541 ttaatttctc acggcggatt atgataatga ttagtctttc cctataatat gaccacgaaa
     9601 tcatttttat attattaaag gtgcgaaaaa atcgggattc ctattggtgt ggatgtgagg
     9661 aaaataaata ctattatatt tatgttaagt gagctctttt ggtctatcac tatgaaaaag
     9721 tatgttaata aaggcacatg gtaatctaat taaaaaattt gttttctata tttgtcttta
     9781 atacattgca actgaattac cagaagttta tacattataa agaaaatgga cgaacagata
     9841 aatcaatatg attatcattc gtccatatgt cttttattaa tataatatta aacgcaaaaa
     9901 atgcctatcc tcttggatag gcatttgtac ggtctcgacg ggaatcgaac ccgcgatctc
     9961 ctgcgtgaca ggcaggcgtg ttaaccgcta cactacgaga cctaaaaaat ggtgactcct
    10021 acgggactcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaagg
    10081 agccgttctt aagcacaaga tagattatag cacagccaat cttgccatga caagatattt
    10141 tttacataat tttgctatta ttttttgaag aatgttataa gtgaaaaatt gcattttatt
    10201 ggcagtctat ttattataat aattacgcta tttttgaaat ttttctattt tagatacttg
    10261 cattcacact tacgtctact ctattttaat agtcattttc gacatcagac ttcctacatt
    10321 caagtatttt atcttaattt tcattaaata tcatctaatc ttctaaatta tgactagcaa
    10381 ataccacctt tacgttagtg ttttttatct aaataatcga atcgatacag aagcaaacat
    10441 aacaaaagcc tcctaccaat aaagatagaa ggctcatgta tttataatca ttgtgtatga
    10501 cttatataaa gttttaccac aattctttta atagatttgt ttgttctcta tctggaccaa
    10561 ctgagaagat agaaatttgt acattacata attctgaaat acgctctaaa tatttacgtg
    10621 cattttcagg taattcttct aaagtacgca cacttgttac gtcttctgtc caacctggta
    10681 gctcttcaaa gattggttta caacgtttta attgatctaa gtttgctggg tactcagtaa
    10741 tttctttacc gtctaattca taagctgtac agattttcac tgtgtctagg cctgttaaaa
    10801 catcaattga gttaatagat aaatctgtaa taccacttac acgacgagag tgacgtaata
    10861 caactgaatc aaaccaacct acacgacgtg gacgtcctgt tgttgtaccg tattcacgac
    10921 caacttctct aatatgatgt ccatcttcat cgaataattc agtagggaat ggaccatcac
    10981 caacacgtga tgtataagct ttacatacac caattacctt tgaaacgaat gtaggaccta
    11041 cacctgtacc aacagtaacg ttacctgcaa ttggattact tgatgtaacg aatggatatg
    11101 taccatggtc gatatctaac attacacctt gcgcaccttc gaaaagtacc ttttcatctg
    11161 ctacaaatgc atcgtctaag atttttgatg tgtctgttac aaattctttt agacgttgac
    11221 ctgctgcata atattcttca aagatatcat caaatgatgg gcatgtttcg ttaaacatgc
    11281 ctttgaaata tgcttgttta tattcaatgt ttgattttaa taatctttcg aatgtttctt
    11341 tttcaagtaa atctgccata cgaataccga tacgttgaac tttgtctaca tatgctggac
    11401 cgataccttt tttagttgta ccaatcttat tatcaccgcg taaacgttct tcatattcat
    11461 cttgtgctaa gtgatatggt aaaatcactt gcgcacgatt agatatacgt aaattacttg
    11521 taggaatgcc acgttcattt aatccgtcta attctttcaa tagtgcaact ggatcaacaa
    11581 cgactccgtt accgattacc gctaatttgt ctttgtaaaa gataccagat ggtactaaat
    11641 gtaatttata tgtttctccg ccaaattgaa tggtatggcc tgcattatta ccacctgaaa
    11701 aacgcgcgat aacatctgac tgttctgcca agaaatccgt tatttttcct tttccttcgt
    11761 ctccccattg tgtcccaact actacgattg atgacatgtg agcacctcca agtttttctc
    11821 atttaaccat ttgatattct accaatatcg agaccataat gcaaataaaa atcgaacatt
    11881 gattttcagc ctttttaaaa gaaaaatcat tttaacaaat cgtcttcaac gtcctcaaaa
    11941 ccctttaaca gagtctttga cagcactaat tcatcacttc caatacacaa acttaacata
    12001 tatcaacccg ttcagaacaa aataaaaacc gtacaatttt atcactaaaa tgattattgt
    12061 acggaaaaac ttttttacat catatcagca tgtgcataat cgatatctgt aaatttatta
    12121 tattgtttca taaagtgtaa cttaactgtg cctgttggac cattacgttg cttagcaatg
    12181 atgatttcaa tttcgccgtt ttcatcattc gtttgcggct cgaaaccacc atcatcgtca
    12241 tcatcttcat cgccaccacg gttatagtaa tcatcacggt ataagaatgc aacgatatcg
    12301 gcatcttgct caatcgaacc agattcacga atatcactca tcattggacg tttatcttgt
    12361 cgttgttcaa caccacgaga taactgactt aatgcgataa ctggacattc taattcacgg
    12421 gctaatgctt ttaatgtacg agagatttca gaaacttcct gttgtctgtt atcagacgca
    12481 cgtgaaccac taccttgaat caactgtaag tagtcaatca caatcatgtc taagccatgt
    12541 tcttgcttta atcgacgaca tttagaacgt agatcattaa ttcgaatacc cggtgtatca
    12601 tcaataaaaa tcttcgtacg tgataattta cctaccgcta tagtaaaacg actccaatct
    12661 tcctcagtca tagtaccggt tcttaagcgg tttgagtcaa cattaccaga actacaaatc
    12721 atacgtgtgg ctaactgatc agcacccatc tctagtgaga aaataccaac tgtatacata
    12781 tcttcatgcg ttgcaacttt ttgtgcaata ttaagtgcga acgcagtctt acctacagat
    12841 ggacgcgctg caaggataat taaatcattt cggttgaacc ctgctgtcat ttggtctaaa
    12901 tctcgatatc ctgtaggaat acctggtgtt tgaccactat tttgatcaag ctcttcagct
    12961 gtttcatata cttgtcctaa gacgtctcga atgtctttaa agccatcgct ttcacgagaa
    13021 gatgatagct ctaaaattcg acgttctgca tcacttaaaa tcgcatctag ttcaagttca
    13081 tcattatatc catcattggc aatactatct gcagtttgaa ttaatctacg ttttaatgca
    13141 tgcttagaaa cgatatcagt ataatactga acatttcgcg tcgttggtac atttgtagat
    13201 aactctgcaa gatattgcgg gccacccgct tcattcaacg taccttccgt cgataattga
    13261 tccatcaatg ttacaacatc aatttcttta ttatcttcat ttaagtgcat cattgcacgg
    13321 aaaatatgtt gatgggcacc cctataaaat gactcaggaa gcaacacttc ctgagtagta
    13381 ttaatcaatt ctggatctat aataattgaa cctaagacag actgttcagc ttcattgtta
    13441 tgtggcattt gattttgctc atacattcta tccatgaatg gttacacctc ttatttcaat
    13501 ccaactttat tgttcaactg tgtgtacgcg aattgtacct tcaacttctt tatctaattt
    13561 aacaggtaca ttcgtatatc ctagggaatg aattccattt ggtaaatcca ttttacgttt
    13621 atcaatttta atatcatgtt gtgcttttag tgcttcggca atttgttttg tgcttactga
    13681 tccaaacaat ttaccacctt caccagtttt tgctgatact tcaacttcaa tgtttgataa
    13741 agtttctttt aatgctttag catcttcaat ttcttgttgg cgttcttgtt ttgcacgttt
    13801 tttctgtaac tctaattgtt tcaggttacc tggtgttgct tctacagcat aattcttttt
    13861 caataagaag ttatttgcat aacctactgg tacttcttta acttcacctt ttttaccttt
    13921 acctttaaca tcttgtgtaa aaattacttt catgcatctt cactcctact taattgttct
    13981 gtaattgctt gttgtaattg cgctatcgcc tcttcgactg tcacaccttt aagttgtgtt
    14041 gcagcattgg ttaaatgtcc accgccacca agtgcttcca ttgttaactg gacatttact
    14101 gaaccgagtg aacgcgcaga tataccaatc agattatctt cacgtctcgc aacaacatat
    14161 gatgcttcaa taccttctaa acttaacagt tcatctgctg cttgtgcaac tgttactgga
    14221 tgataaattt tatcgtctga accatgcgca atggctatgc cattatcttc aacttttaca
    14281 gttcgaatta attcagatcg attaatgtaa gtatccacat catcttttaa gaaatgttgc
    14341 gttaaaatcg tatctgcacc atgtgcacgt aaataactcg ctgcatcgaa tgttcttgat
    14401 cctgttcgta atgtaaagtt tcttgtatct acaataatac ctgcatacat cactgttgat
    14461 tcaagacgcg ttaaacgttg ttctgttggt tgatattcca gtaattctgt tacaagttct
    14521 gctgttgaac ttgcgtatgg ttccatatat atcaacaatg gattagagat gaagctttca
    14581 ccacgtctat gatgatcgat aacaacttta cggtttgctt tatttaagac attttcatct
    14641 aaaaccagtt ccggtttatg cgtatcaaca atcactacgg ttgtcttaga tgtcatcata
    14701 tcccaagcat catctgatgt aataaatcgc tctcttaact ctggcttttt atcaatttcg
    14761 ttcatcacgc gtcgtaatgt tggatcaatg tcagtctcat ttaatacgat gtatgcttct
    14821 aaattattca tcattgcaaa tctagacaca ccgattgctg caccaattgc atctaagtca
    14881 ggacgtttat gtcccatgat aatgactttg tcaccctctg caaggatgtc ttttaacgca
    14941 tgtgagataa cacgtgctct tacacgagta cgtttctcca tcgggtcagt cttaccgcca
    15001 tagaagcgca cattaccatt aatactttta attgcaactt ggtcaccacc acgtcctaat
    15061 gctaagtcta ggcctgattg tgataattca cctaagtcga ttaaattttc agtgccttca
    15121 ccaacaccga tacttaatgt taattgggca cgataaccaa cacttttttc acgtaattga
    15181 ctcaagatat caaatttaga ttcttctaag tcagctaata ttttttgatt taaataggct
    15241 acgaattgat cggaactata ccttttaaag aatatattat actcagttgc ccatcgacta
    15301 atgacacgcg ttaccattga attgatctcc gaacgctgcg tatcattcat attttgcgta
    15361 atctcatcgt agttgtctaa aaataatgtc gcaatgattg gtttagaatt ttcatatagt
    15421 tcatttgttt gtacttgttc agttatatca aagaaataga ggcagtgatc attctcagaa
    15481 taacgtactt ggaaatgata ctgattatat tctatttcaa cggatttcac tctatctaat
    15541 tgctttaaaa tgtttggaaa tacttcattt acagattcag aaatgacatt tgcttccata
    15601 tgatctgtca taaattggtt aacccattcg atgtgatcat tttcatctaa aacaatgata
    15661 ccaattggta aatgtttgat tgctttatta tttgttgttg aaatttgagc actcaaacca
    15721 tctacataac tatccatttt cattaaagct tgtctgaata aaacgatgct aacaataatc
    15781 atcacgacaa gaacgataga tgcaattaat gctataaggc tattaaagat aaaccataca
    15841 cccattaaaa caattgctgt gatgatcatg atgacaaatg gtattagtaa agctttctta
    15901 gtggactgcc gattcattat tccacctcta ttcacttttt agaattattt ttcatgattc
    15961 gcttcaaatt caaacttaaa tcgataacac caagtagtcc tacaatatgc gtcgtaggtg
    16021 tcagtattgt accgataacc aatagtaaaa tcgttactgc attcggcaaa cctttcgctt
    16081 taccaaagaa atgaataaca cttaaacctt gaatatacat cactaatgat aacacaagtt
    16141 ggaaatttaa aagaatgctc tggaacacac tcggttgacc tgtaaataat aaacatatga
    16201 taacaataat gtatatccat aataaaatac cgctcatttg ccacgcgaaa agtggcttaa
    16261 atacaggtgt agcgatttta aattttcgta aaatcggaaa tgtaacgatt aagttaatta
    16321 agacgattaa aaatgtaatg ataatgatga aacctggtaa ttgaacggtc gcttgtctaa
    16381 acccttcttc taatatttgg gtcatattcg catcggcacc gctcatcgta atcgcttcat
    16441 gtaatgtttg cttgaaaggt tttactatgc tcgctgatgg tggaattctt ccgaatgttt
    16501 gtagtaacat aaaggcgatt aatgaaatta agctcatcgc tacggttgtt acgtataata
    16561 ttctttcttt agacgttctt tctttgagca attgaccaat aattaaactt gcaattaaga
    16621 ctaatatgat agcacttaaa acgaaagtat tacctaaaac agttgttata attactgtaa
    16681 taagtgcact aatcccgaaa gattgtattg atttattcca taaaacgata cctggtatgg
    16741 ttgcaaataa tgcaaatatt agtcctaaac caggcactag atataaagct aaagcgacaa
    16801 ataccaacgt aatcgttgca atcattgttg ctttaggttg tatttttgaa aacacataag
    16861 ccactcccat atttttaact atagctatta ttttaacctc tttaatggaa attaacaatt
    16921 tatagattgt atgcttctat ttcatttaat tgaataataa ctttcatgtt ttataagtaa
    16981 ttaacatact catttaaatc gcttttgtgt gctttcattt tcaacatgat tatttaatcc
    17041 cactacatag caatcaagct tgatttagat ttacaattca tttccactct catgtactct
    17101 agatgttttt gaatatgata actgtgattt agtggcttca ttctttgaaa atatatacat
    17161 tattacttgc gcttaaaatg ctttaaattt aagaaatgat ataagttagg tgcccaggta
    17221 ctaaagttta gtaggaatcc atcatgccca acgttatcag gtacaaagaa atgacgatga
    17281 tatttaaaac gttcacctaa agcacgaact tgatcatccg gatatagcaa atcatctata
    17341 aagcccattg ttaagacttt cgtttctaaa tttttaaaaa catgcgttac gtctgtgcga
    17401 cctcggtcaa tgttgtgact atccaataca tctagcagtg tcagataaca attcaaatcg
    17461 aatcgttctt taaatttatt accttgatgt tgctggtatg cgactacttc atccggtgtg
    17521 aaacgttcat cataactttt cgatgatcga tatgtcaaaa aacctaattg gcgtgcaata
    17581 cttagacctt ccttaccacc aagatgaatg gcctgacgcg caatttcatt gaaagctcta
    17641 ctataagatg atgttcgact tgttgcagca agaataatgg ctttatctac ttcaaactgt
    17701 cgattgtaaa gtagttccat tgcttgcata cctccaagac ttccccctat taaaatatta
    17761 attttgtcat aaccaagcgc ttgtatacct cgttcattcg ctctaacaat atctcttaat
    17821 gttaattttt taggaaaatg agggtcgttt aaaggtgaac ttgaaccgaa aggactacca
    17881 ataacatcaa atgttaaaaa ttgatagtca tgaataggca catatccccc atcaataatt
    17941 tctcgccacc accccggata atcatctgtt ccatatgtta aatgattgcc agttaatgca
    18001 tgacaaacta caactaatgg ttgtccatga taaccgacat gctcatatct caaacgcaag
    18061 ttatctatga cttccccaga ttctgttata aattccccta aatttaaagt atctactgtg
    18121 taatttgtca ttgttctttc ctccttaaac aaaaaacttc tcaccctatt gaaaagtaag
    18181 aagtctttat acttatcatt cgagtaactc gttggtttta gcaccgtgct ataaagtcgg
    18241 ttgctgaagt atcacagggc caagtccctc aacttctcac aataagaaat atgaaattgt
    18301 tatgtgttag ttgagattca gtgatgaatt acttttatca tttaaaatgt tgttatcatt
    18361 gtcatgcgtt agccaaatcg cttacgtata cacgattccc aatcttaaca tagatgattt
    18421 gtatatcaga attttctgat tactaacagt ttacctaagt ttaaatatct gttcaatgat
    18481 tttcagttat ttttaaaaga aaaatcgtaa tgctgccatg ataacaatcc cactaataat
    18541 tgtaatagtt aaactacgcg tgattataga taaaataacc gtcggaatga gcgcgataat
    18601 gtaagggatg tttaatgtat acccctcacc atgaggcgtc tgttgaataa tgctgtcaat
    18661 gacaagtgcc gtaaatagtg tgattgggat aaatgatagc catcgaacca cgacatcagg
    18721 caattgcact tttgaaatca tgataaaagg tataattcga attaatagcg ttacgattcc
    18781 acacaataaa ataagtatta acatgttcat atgagttatc atcgttccat catcactcct
    18841 aacgctgctg aaattgtggc tgcaattaat attgctagat atgaaggcat aaacatactt
    18901 agcgataaca tcattactat gacggcaata atgagtacta tgtaaattct taaacgcgat
    18961 ttagtaattg attcaaattg tgcaatggct aaaaagataa acatagccgt gatagcaaaa
    19021 tctaatccta atgcttgtgg attcgaaata tactcgccaa acaatgcacc tgctacacat
    19081 ggaattaccc aaaataaata tgctgtaata ttaagaccat gcatccaacg atcattgata
    19141 gcttctcctt ttaaataagg tgtaatggcg acaccaaacg tttcgtcagt tactaatgaa
    19201 cctaatccaa cacggttcca aaacccatat gtcttgaagt ttggtgcaag cgacatactt
    19261 aaaaggaaca ttcttgaatt tacgataaat acagttagta caatcgctga tatcggtgta
    19321 cctgctataa acaacgcgca cataataaat tgcgcagcac cggcatatat aacaagacat
    19381 aacaagataa tttctaaaat actaaagttt tgagatgaag ccacgatacc aaatgaaata
    19441 ccaacaccgg cataacccaa taatgttggg atacactctt gcacgccttg tctaaaactc
    19501 aaatgtgttg tcatctcaat tacctccttt ttcactttat atttatataa ttacgaaaaa
    19561 ataatatacc tagtctaata tagttctata tgacaagcaa taacgttaga ctccggtttg
    19621 tctgaggtta gacataccgg agtctttaat cggcttcata tcagcttttt agttttgaga
    19681 attttcaata ttttatttaa tactacctcc atattttcta cgggtttgta gttgttttta
    19741 agtactaatt ttataatttt ttatatctaa tgatgagtct gggatattga ttcatgtctc
    19801 taacactttc gataatcccc cctaccctac tgcatcgata ttactcatac tacatcaacg
    19861 aaatcagttt tttatcactt aatttcccat aatagtgatg ctcaaaattg ttacgtttta
    19921 gattgtttta gttcatcata attatcccgt attgttgcta taatgaaatg cgttcacccc
    19981 attaaacaac aaacttaatt tattgttgtt atgtgcattg gctcactatt atacttttac
    20041 agcacaaaaa aagtggcgac agcttcgtca ccacttttta aaatattatt taaagtatct
    20101 tggccttgct ttaagtatac gtagatatat actttttaaa gcttgtagct aaagtcttta
    20161 tttaactggt tttgaaattt gtgttttacc acccataaat ggtactaatg cttctggaat
    20221 tgttactgtt ccatcttcat tttggtaatt ttcaacaata gcagcaaatg tacgtccaac
    20281 tgctaaacca ctaccattta atgtatgtgc taattctggt ttagctgctt tgtcacgctt
    20341 gaagcggatg ttagcacgac gcgcttggaa atccgtacag tttgagcatg aactaatttc
    20401 tttataatca ttgtagcttg gtaaccaaac ttctaaatca tatgttttgc ttgcactaaa
    20461 tccaatatca cctgtacata aaataacacg acggtatggt aaacctaact cttctaaaat
    20521 tgcttctgcg tttgttgtca tttcttctaa agcattccat gaatcttcag gttgttcaaa
    20581 acgtaccatt tccactttat cgaattgatg taaacgaatt aatcctcttg tatctctacc
    20641 tgctgatcct gcttcactac ggaaacatgc agattgacca gtgaattttt caggaagtac
    20701 acctggttga ataatttcat tacgatagaa attcgttaat ggtacttcag cagttggaat
    20761 tgtatataat ccttcttttt ctactttaaa taaatcttct tcaaatttag gtaattgacc
    20821 tgtaccatac attgtatctg cgtttacaag ctgtggtacc atcatttctg tataaccatg
    20881 ttgtgttgta tgttttgtaa tcatatagtt cattaaagca cgctctaatt gcgcaccttc
    20941 atttgttaaa tatacaaaac gcgcacctga aacttttgct gcacgatcaa aatcagccat
    21001 tttcaattct tctacaatat cccaatgtgc ttttggttca aatgaaaact cacgtggtgt
    21061 accccacttt ttaacttcaa cgttatcttc atcagattca ccttgaggta catcatcact
    21121 tattaaattt ggaatacgac aaaggatacc tgtcatttta ctatcaattt catttaattg
    21181 actatctttt tctttaatat cgtcacctaa tgtgcgcatt tcagcaatca catcatcagc
    21241 attttcttta ttacgttttt ttaatgcgat ttcttcgctt actttattac gacgtgcttt
    21301 catttcttct gttgcactaa ttaatttacg tcgttgctca tccaattcta aaatttcatc
    21361 tacaactttt ggatcatctc cgcgtaattc aattttgctc ttaactgtgt caggctcatt
    21421 tctgaataat ctaatgtcta acattaacct tcatcctttc ccaaataatt atcatttatt
    21481 atggaatgac gcacgtcttt attttttaga aaataaaaaa agaccacatc cctacaaggg
    21541 acgtggtcta cgcgttgcca ccctatttaa caatttaagt tataaagata cactaaacct
    21601 aaattgcact tcactaaaat aacggttatc accgattgtt cttttaaata agtaggtaga
    21661 ttcatatata tgttgattct tgttcacact aaccacaagc tctctgatat cgaacactat
    21721 atattacttg tcctacgaac aatgtcttat taagttattt ttaatatagc aaactatatt
    21781 tgctttttca agtaacgatt tcaaacatca atcatgtcga tttagtgaca tgcagtcgtt
    21841 tgataaattg attgctttaa atactgtgca accgcttcaa tatctttatg aaattgacgg
    21901 tcatatgtaa tggatggcac gatacttcga aactcatcat acttgcgacg tgtttttggt
    21961 gataatcctt cgacaccttt caactctgct gcttgtaatg caataacaca ttcgattgcc
    22021 aacacacgtc ttgcattttc aataatttga taaccatgtc tagcagctgt agttcccata
    22081 gatacgtgat cttcttggtt cgcagatgaa gtgatagaat caacactcgc tggatgcgct
    22141 aaagttttat tttcagaaac gagacttgca gcagcatatt gcataatcat cgcgccactt
    22201 tgcaatcctg gctctggact aagaaatgct ggtaaatcac catttaattg aggatttact
    22261 agtcgctcta gacgacgttc cgatacgttt gctaattcac ttacacctaa tttaagatga
    22321 tctaatgcaa aagcaatagg ttgtccatgg aagttaccac ccgaaataac aaacgttgca
    22381 tttgcttcct caaatataag tggattatca ttagccgcat tcatttcaaa ttctaattgc
    22441 tgtttaacat aattgaatac ttgaaaactc gcgccatgga tttgtggtat acaacgcaat
    22501 gtatatgcat cttgtacacg tatttctgct tgtcgcgtcg ttaatgttga tccttctaac
    22561 caatcacgca tacgcgctgc cacattaatc tgttcttgaa aattacgaac agcgtgcaca
    22621 tcatgtcgat atgcatctat aatgccatta agagactgat gcgttaatgc agcaatccat
    22681 tcagattggt aacctaaatc ttctgcttct atataactaa tgacaccttg agctgtcata
    22741 gcttgcgtac cattaatcaa tgctaaacct tctttagcct gaaggttcaa aggttgtcta
    22801 tttaattctc ttaatacatc gtcactatcc ttttcttcac ctctgtacaa tactttccct
    22861 tcaccaatta atgctaatgc taaatgtgat aatggcgcta aatctcctga tgcaccgaga
    22921 gagccttgct gtgggattat cggtataata cgttcattta taaaaaattg taattgtctc
    22981 actaattcta aagtggcacc tgaatgacct tttaataatg tattcaatcg taaaatcatc
    23041 atgactaatg ctacttcttt tgaaaatggc tcacctagtc cacaggcatg tgagcgtatc
    23101 agattcattt gtaattcgtt atattgcgtc gggtctatac gtacatcact aaataaccca
    23161 aaacctgtag tgataccgta aaccgtttcc tcattttcaa taatacgttc aactactgct
    23221 ctactttttt tgacacgttc taacgcatca tcaataattt caatctttga ttgttgttgt
    23281 aaaaatgatt taatatactc aattgttagt gtttcaccat ctaaatataa agtcatatat
    23341 gttaccccct tgtttatatt aagtaaccca tccttcttga agtatacgtt ttcattttta
    23401 ttgaaacaat ggttttacgt acatttataa cctattatca gagcactatt gtagtgcgtt
    23461 aaaggatatt aagattgttg taagcatatt taa
//
LOCUS       DATTQF010000025        22850 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.025, whole genome shotgun sequence.
ACCESSION   DATTQF010000025 DATTQF010000000
VERSION     DATTQF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.025"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1103)
                     /locus_tag="VB241_000911"
     CDS             complement(<1..1103)
                     /locus_tag="VB241_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEP1244218.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPLALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDQIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAFFQSKKAN
                     PDIENGFIYTGDVHIILVQILCVTAVVIFSIVMT"
     gene            complement(1312..1536)
                     /locus_tag="VB241_000912"
     CDS             complement(1312..1536)
                     /locus_tag="VB241_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEP1244219.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITVQKV"
     gene            complement(1596..2675)
                     /locus_tag="VB241_000913"
     CDS             complement(1596..2675)
                     /locus_tag="VB241_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEP1244220.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITVQSIGLLILTTTGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGAREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVQQQTQIV"
     gene            complement(2981..3616)
                     /locus_tag="VB241_000914"
     CDS             complement(2981..3616)
                     /locus_tag="VB241_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP1244221.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            3869..5797
                     /locus_tag="VB241_000915"
     CDS             3869..5797
                     /locus_tag="VB241_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1244222.1"
                     /translation="MILLQLNHISKSFDGEDIFNDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNLSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEHALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            complement(5942..6070)
                     /locus_tag="VB241_000916"
     CDS             complement(5942..6070)
                     /locus_tag="VB241_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244223.1"
                     /translation="MIKTMSIENMRTYQQRVYNLWIKGVDNVIYCGYQVGKQKIND"
     gene            6165..7775
                     /locus_tag="VB241_000917"
     CDS             6165..7775
                     /locus_tag="VB241_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HEP1244224.1"
                     /translation="MSTNQTFLIFVIAIIILTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLESFIRPNSHFDYEYKLYKDKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRHNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHINELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVSNG"
     gene            complement(8089..9114)
                     /gene="tsaD"
                     /locus_tag="VB241_000918"
     CDS             complement(8089..9114)
                     /gene="tsaD"
                     /locus_tag="VB241_000918"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP1244225.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLTFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(9107..9571)
                     /gene="rimI"
                     /locus_tag="VB241_000919"
     CDS             complement(9107..9571)
                     /gene="rimI"
                     /locus_tag="VB241_000919"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP1244226.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(9544..10206)
                     /gene="tsaB"
                     /locus_tag="VB241_000920"
     CDS             complement(9544..10206)
                     /gene="tsaB"
                     /locus_tag="VB241_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP1244227.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIDALRAFLEDLNQPFIY
                     IGKDTVKLQDDLQGDVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(10187..10648)
                     /gene="tsaE"
                     /locus_tag="VB241_000921"
     CDS             complement(10187..10648)
                     /gene="tsaE"
                     /locus_tag="VB241_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP1244228.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENSRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            10686..10841
                     /locus_tag="VB241_000922"
     CDS             10686..10841
                     /locus_tag="VB241_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244229.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIISSKLIVRKFKIF
                     VDILKQI"
     gene            11159..12847
                     /gene="ilvD"
                     /locus_tag="VB241_000923"
     CDS             11159..12847
                     /gene="ilvD"
                     /locus_tag="VB241_000923"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEP1244230.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHSLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLKVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            12875..14644
                     /gene="ilvB"
                     /locus_tag="VB241_000924"
     CDS             12875..14644
                     /gene="ilvB"
                     /locus_tag="VB241_000924"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEP1244231.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILAIHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVKTIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDTAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            14644..14898
                     /locus_tag="VB241_000925"
     CDS             14644..14898
                     /locus_tag="VB241_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEP1244232.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            15020..16039
                     /gene="ilvC"
                     /locus_tag="VB241_000926"
     CDS             15020..16039
                     /gene="ilvC"
                     /locus_tag="VB241_000926"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEP1244233.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIEK"
     gene            16069..17598
                     /locus_tag="VB241_000927"
     CDS             16069..17598
                     /locus_tag="VB241_000927"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEP1244234.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYIRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVIKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            17601..18647
                     /gene="leuB"
                     /locus_tag="VB241_000928"
     CDS             17601..18647
                     /gene="leuB"
                     /locus_tag="VB241_000928"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEP1244235.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKAERVEGTDLVIVRELTSGIYFGEPRYFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            18661..20031
                     /gene="leuC"
                     /locus_tag="VB241_000929"
                     /pseudo
     CDS             18661..20031
                     /gene="leuC"
                     /locus_tag="VB241_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
     gene            20032..20604
                     /gene="leuD"
                     /locus_tag="VB241_000930"
     CDS             20032..20604
                     /gene="leuD"
                     /locus_tag="VB241_000930"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEP1244236.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            20619..21887
                     /gene="ilvA"
                     /locus_tag="VB241_000931"
     CDS             20619..21887
                     /gene="ilvA"
                     /locus_tag="VB241_000931"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEP1244237.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(21964..22380)
                     /locus_tag="VB241_000932"
     CDS             complement(21964..22380)
                     /locus_tag="VB241_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244238.1"
                     /translation="MKIFIGTMLTFLFVCSLIVCGFFAYKIYFPTDAENKSEIPNHMQ
                     ENKHEKENTHNINNNGSEANENFNNNKNRIENIEDKQKAYALMSIPRHNLPESEKAIY
                     DKVISTAIQNINNGVGNEEDFRLKEIYDGIQSVQPQ"
     gene            complement(22708..22822)
                     /gene="rrf"
                     /locus_tag="VB241_000933"
     rRNA            complement(22708..22822)
                     /gene="rrf"
                     /locus_tag="VB241_000933"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         7777 a         4008 c         3807 g         7258 t
ORIGIN      
        1 gtcatgacga tgctaaaaat tacaactgct gttacacata atatttgcac aagtataata
       61 tgcacgtcac cagtgtaaat aaaaccattc tcaatatctg gatttgcttt tttactttgg
      121 aaaaaggctg ttaatactgc accaataata ccgccaacac cgtggatacc aaatgcatct
      181 aatgcatcat gatactttaa ttttaccttt atgtaattga tgacaatata acaacagata
      241 ccccctatta aagccatgat tgttgcacta agatatgtta catatccagc tgcaggtgta
      301 ataacaacta atcctgctaa tgcaccgagt aaaagtccaa gtaaacttgt cgtcttttta
      361 aaaatatatt ctaaaattaa ccaaccaata gccccggcac tggcagaaat aactgtattc
      421 gtaaatgcaa gcattgcaat ttgatcaaat gtaaaagcac tgcctacatt aaatccatac
      481 caaccaatcc acacgaatat accgccaatc aatgtaatga taagattatg tggtgttgat
      541 tcagaatgtt tgtttccttt tccaatcatt atagctaata ctaaaccaga aacacctgat
      601 gtaatatgaa cgactgtacc tccagcgaaa tctaatacac cgagtttgtt aatccaaccg
      661 ccgccccaaa cccaatgtgc tactggactg tatacaagag cagtccatat tactacaaat
      721 aataaataag gaataaactt cattttctca gcgattgaac ctgataatat tgagattgca
      781 atcgtacaaa acatcatttg gaataacata aataatgcca atggaatatg cggactaata
      841 tcttcttgtg tcgcgaaacc tacatgatta agaaatgtat attcccagtt accaaaccat
      901 aaattccctt ccccaaaact aattgtaaaa cctactgtta tccatacaaa tgtaacaagc
      961 acaattgctg ccatactttg catgacagta tttagcgcat ttttagattg aactaaccca
     1021 ccataaaata aacttaatcc tggtgtcatt agccaaacta ataatgtaca caaaaacata
     1081 aatatcgtat cgttaagatt catacttacc actcccttgt ttccataata atctgataat
     1141 ttataaaaag caaaaatgcg taaagtttta ttacatcttt tggaacaaaa tgtgccacgc
     1201 tatataaaaa atgatttcat gaactaaaac aaaaaagact acttactcgc ttttaaagta
     1261 gcggaagtag ccatagaatc taatatcaat tttattatta aaatgataac gttaaacttt
     1321 ttgaactgta attgtccatg aagcattatc aatttgttca tagtttgtta caggatatcc
     1381 attttctgca gcccaatttg gaatagcttc cgtcgcttgt gtgcaatcaa aatcaatttt
     1441 taattcatct ccagattgca atgttgccat tttcttttgc gcttcaatta acggaaatgg
     1501 acataccatt cctactgtac ctaattcgtg tatcatagct atttctcctt ttccaatcac
     1561 ttttatttga tcaacatcat tggcataata accttctata ctatttgcgt ttgctgttgg
     1621 acctttgcag attgttgttg tacttttttc attggacgaa caaagataaa atgactcatt
     1681 gtccataccc caactatcat cgatgctagc gcaatccatc cttgccaagt catcgttgcc
     1741 gtttcaacca aaccgttacc gatagaacaa ccaccagcaa ctgacgcacc aaatcccata
     1801 catataccac caatagcact gtttcgtatt gtaatcttgt ctggcaatcg ccatttaaat
     1861 tctcttgctc ctctagctgc aatatatgaa ccaatgaaaa ttcctagaac taaaaagaca
     1921 ccccaatcaa taaatttagt ttcacctgta atcaaaaagt gtactaaatt tgctgatggc
     1981 gttgttatac ctaaaccgtc atttcttcca gttgatgcac tcattggcca agctaagagt
     2041 gcgataagtc caattgcaat tgctgcaata aatggatggt atcgtttttc gaaaaggtaa
     2101 tatctaatac ctgtataacg ttgctttagt tttggaacag caactcgaac tttcttatta
     2161 ttgagcgttc ttacaacgag aaaaatggtg attatagtta atataacgac taatccccaa
     2221 aacggaatgc ccgttgtttg agacatatca ctatttacat tcgttggttg attaatttta
     2281 tccattactg gctttaaaat ccctgtttta gtgattgctg cagtaacagc atataatact
     2341 aatgcaatcc aactaccaat tagcccttca ccagcgcgat accaagtacc tgttgcacat
     2401 cctccagcca atactattcc aattccaaaa ataaaagaac ctataactgt tcccaatatt
     2461 ggaaaactat gtgcaggaat ttgtaaaata cctgttgtcg tcaaaatcaa taatcctata
     2521 ctttgaacag taatagcgat taataatgca tagaacatct tattattctt ttgcacatac
     2581 atatctcgaa agccacctgt taaacaaaat cttgttcttt gcatcacaaa acctaagagt
     2641 cccccgacaa ttaagccact aaaaatcatc cacataatcc taacccccct aatttccgat
     2701 aagtttagta tgtattatag gcgatgattt tcttgttttc aaccttatac acaatatttt
     2761 tgatatttat attatgttca tataagaaat gcgattaatt acgattcatc attatatctc
     2821 tacgtgttaa tttattaacc tatattatat ttatcaacac aacagttttc cctttcattt
     2881 tcaaacataa cagaaaaagc ctgagacata cttagtccca gactgttcta taattaatta
     2941 aagatataac aaggttaatc attcaaagct aacatcacat ttattcactg taatttttca
     3001 taaagaataa taatgactgt aattctatac ctaagtcaat ttgatgtact tgcacatctg
     3061 aaggcgtatt tattctacca ggagtgaagt ttaaaatacc tttcacacca gcttggacga
     3121 gttcatctgc aactttctgt gcaactcttt ctggtgtagt tagaatcaca acatctattt
     3181 cttccttctt caatgttgtt attaattcat cgttatcttt tacaataacg ttccctattt
     3241 tctggccaat aacatcttct tttacgtcaa acgcttctgt aatcgtcata tcgtcatgta
     3301 ttgaaaagtt atatgtgagc aaagctttcc ctaggttccc aactccgaca attgcgattt
     3361 tgatcatgtc actctcgctt agttcagatt taaagaaatc caataaacta tctatattat
     3421 atccgtaccc ttttttacct aattcgccaa aatatgaaaa gtcacgacga attgttgccg
     3481 agtcaatttg taacgcatcg ctaatcgctt ttgaatttac acgatctata cctttagatt
     3541 ttaatgaact gacaaatcta taatataacg gcaaacgttt taaagttgct cgaggaattt
     3601 taacttggtc actcattcgc tatttcctcc ttcgtgtttg aatgaattat atctatgtat
     3661 tcaaacgagt tagagcgtaa ttgttagatt attaaaaaac aaataattga ttacaatgaa
     3721 tacctaaata ttatacatta ttgaatttta aaaataaata tcttagtttc aagacggcgt
     3781 tttatactgt aaaatggtta ttaatgtaat aattacgact tttatcataa gttaataaaa
     3841 tttgcgaaag acgaaaggtg aagatcgaat gatactttta caactcaatc atatatcaaa
     3901 atcgttcgat ggtgaagata tatttaatga tgttgatttt gaagtaaaaa ctggtgaacg
     3961 aattggtatc gtaggaagaa atggcgccgg taaatcaaca ttgatgaaaa ttatagctgg
     4021 cgtagaaaac tatgattctg gaaatttgtc aaaaattaaa aacttaaaac taggttattt
     4081 aactcaacaa atgactttaa attctaatgc aacagttttc gaagaaatgt caaaaccatt
     4141 tgaacatatt aaacgaatgg aaagcttaat caaagaagaa acagattggt tatcaaaaca
     4201 tgcaaatgat tacgatagtg atacatataa aacacatatg tcccgttatg aatctttatc
     4261 gaatcaattt gaacaattag aaggatatca atatgaaagt aaaattaaaa cagtacttca
     4321 cgggttgaat tttagtgaag aagatttcaa taaacctatc aatgatttta gcggtggcca
     4381 aaaaacacgt ttatctttag ctcaaatgct attaaatgaa cctgatttat tacttttaga
     4441 tgaacctact aaccacttag atttagaaac gacaaagtgg cttgaagatt atctacgtta
     4501 ttttaaaggt gcaatagtca tcatcagcca tgatcgttac tttttagata aaatagttac
     4561 tcaaatttat gatgtggctt taggtgatgt caaacgctat gttggtaatt acgaggaatt
     4621 tatacagcaa cgggatttat attatcaaaa acgaatgcaa gaatatgaaa gtcaacaagc
     4681 agaaataaaa cgattagaaa cttttgttga gaaaaatatt acccgtgctt caacaagcgg
     4741 aatggcaaaa agtagacgta agattttaga aaaaatggaa cgcattgata aaccaatgtt
     4801 agatgccaaa agtgcaaata ttcaatttgg ctttgaccgg aatacaggta atgacgtcat
     4861 gcatgtaaaa aatttagaaa tcggttatca aactgcaatt accaaaccta tcagtataga
     4921 ggtctctaaa ggcgatcata tagcaatcat tgggccaaat ggtattggaa aatcgacctt
     4981 aattaaaact attgcaaatc aacaaaaagc gcttaatggc gatattactt ttggcgcaaa
     5041 tttacaaatt ggttattatg atcaaaagca agcagaattt aaatctagta aaacaatttt
     5101 agattatgtg tgggatcaat atccgttaat gaatgaaaaa gatattcgtg cagttcttgg
     5161 acgtttctta tttgtacaag acgatgttaa aaagataatt aatgatttat ctggtggtga
     5221 aaaagcacgt ttacaactag cactacttat gttgcaacgt gacaatgtac ttattttaga
     5281 tgaacctacc aaccacctcg atatagattc aaaagaaatg ttagagcatg cactccaaca
     5341 ttttgaagga acaatactat ttgtttccca tgatcgttac tttattaacc aattagcaaa
     5401 caaggtattt gatttaacaa ttgatggcgg aaagatgtat ttaggagatt atcaatatta
     5461 cattgaaaaa gttgaggaag cggaagcgtt gaaagcgcac caagaagaac agtcagttaa
     5521 cgtacaagca catgaaaagt ccatggaaca atcgtcgtat cataaccaaa aagaacaaag
     5581 acgtgaacaa cgcaaacttg aacgacaaat atccgagtgt gaaaatgaaa tagaaacttt
     5641 agagactact atcttacaaa tagacgagca attaacccaa ccagaagttt ataacaatcc
     5701 acaaaaagca aacgaattag ctatccaaaa acaagatagc gaacaaaaat tagaacacgc
     5761 tatgtctaaa tgggaagaat tacaacaaaa actataaaaa tatacataac tctaactgta
     5821 aaatcgctat agttagagtt atttttttac aaattttaaa tttttttatt cacaataaaa
     5881 acgctgatat tatagctatc cacagagtta tcctagttat ccacaagtaa attaacacta
     5941 atcaatcatt tattttctgt tttcccacct gatatccaca ataaatgacg ttatcaacac
     6001 cttttatcca caagttatac actctttgtt gataagtacg catgttctct attgacattg
     6061 tttttatcaa aatccattgt taagacattt tctttatgaa ataataataa taatattatt
     6121 gaagaatatt tttattatta ttaaaaataa ataaggagat agttatgagc acaaatcaaa
     6181 catttttaat atttgttata gcaattatta tacttacatc ggtaatagga attgttggac
     6241 gatacatgag tcgtcaacgt ctattaaaat ctatggaaac attatggcaa acgatttctc
     6301 cattagagtc ttttatcaga ccgaactcac atttcgacta tgagtataag ctctacaagg
     6361 ataaatttga atcacattct ttagttgatg ataaaacttg gtccgattta aatatgaatg
     6421 caatctttca taagatgaat tataatttaa cagctattgg cgaaatgaaa ctatatgcct
     6481 gtttacgtgg aatgctttca attacgaaca aatcattact tagtttattt aatgataatg
     6541 ctgaatttag aaaaaacgta acatatcatt tagctttgat tggtaaaact gtttatccaa
     6601 catttccaga ccaaattaca ccggtaaaac gtcataatat attgtttcta tgcccgtttt
     6661 taccagttat ctcatttgca gttatattta ttaattcaca agttggtatt ttattatttc
     6721 ttatgagttg cctatttaat atcattttat ctgcaacttt aaagcgtaca tatgaggatg
     6781 acttaaagtc tatattttat gcatccaatg ttttaaaaca aggctatact atctcaaaaa
     6841 ttaaacatgc tccacaaccc gaagtcaatt ttaagcaatt tagaacagca cgtcatttaa
     6901 cgagtgtttt agctgaagtt aatgatgaag atatcggagc tatggtaatt aaactagtta
     6961 aacttatttt catgcttgat tatgttttat tccattcaat tcaaaaaagc tatacaacac
     7021 atataaatga gctaaaaaat tgtttcgatt acatagctga attagataat cattatgcat
     7081 tagcaatgta tcgtagaacg ctagaatgct ataccgaacc acaaattgat gattcaaatg
     7141 atggcatagt attctccgag ttaacacatc cactcatagc agatgcagta gccaatgatt
     7201 tctcactatc acaaaatata ctgttaactg gatctaatgc ttcgggtaaa tcaacattta
     7261 tgaaatccat agcaataaac atcattcttg catcggctat acaaacagtt accgcaagca
     7321 aatttgtata tcaacctgga attgtgttca cgtcaatggc aaatgccgat gatgtgttat
     7381 caggcgatag ttattttatg gctgaattaa aatctattaa gcgtattgtt gaaattcctg
     7441 ataatcaaaa aatatactgc tttatcgatg aaatttttaa aggtaccaac acaactgaac
     7501 gaattgccgc ttcagaatca gtactatcat ttttacatga aaaatctaac tttagagtta
     7561 ttgcagcaac acatgatatt gagttagctg aactcttaaa acaacgttat gaaaattatc
     7621 atttcaatga ggtaatagaa aataataaca tacattttga ttacaaaatt aagcctggca
     7681 aagcaaatac acgtaatgcc atcgaattat taaaaatcac ttcatttcca gcaaaaatat
     7741 atgaacgagc aaaagataat gtttccaatg gttagcatga atcttgaaca tcaatcaatg
     7801 ttcaagtcgc aacattgtta tattggcagt ctttgaccaa atgaaaatgc gcttgtaaca
     7861 agttttttca atactagtcc gcggccccaa cacagaagct ggcggaaagt cagctttcaa
     7921 tcgtgtacaa gttggcgggg ccccaacata ataaatcatt agtggttctt tatcttttct
     7981 gtctcactcc cctaattgca atataaaaac gtcagctatc acatgacaga agacaatgaa
     8041 cagtttcaat aatattcatc gtaatcatgt taataactga cgtttatttt attctgcaga
     8101 atactcttct aaatctatat tgctgtgccc atttaatgct aaatcagcaa atcgaccttg
     8161 ctgatacaaa tagtggccgg caacgcctat cattgcagca ttatctgtgc ataatttagg
     8221 acttgggata gttaattgaa tgtcattgac tttgcattga tccgctaaag attgacgtaa
     8281 tcctttatta ctcgccacgc caccagcaac aattaatcgc tgaacaccat attctttaca
     8341 agcttgaata gctttaaacg tgagaacctc tacaacactg ttttgaaagc tcgttgctac
     8401 gttagcttca atgattggaa tatttttttg tcgttgattg tgaagttgat tgattacggc
     8461 acttttcaac ccactaaaac taaaatcata actatcttta tccaaccaaa cacgagggaa
     8521 tgaataagta tcttcacctt cagcagccaa ccggtcaact tgtggaccac ctggataatt
     8581 taaaccaatt gttcgtgcca ctttatcata agcctcacct actgcgtcat ctcgtgtttc
     8641 accaatgact tcaaatgtta aatgatcttt catataaact aattcagtat gtccacctga
     8701 aacaataagt gcaattagcg ggaatgttaa tggctcttct atgtgattag catatatatg
     8761 tcctgcaata tgatgaacag gaataagcgg cttatcgtaa gcaaatgcca atgctttggc
     8821 tgcattaaca cctattagta acgcaccaat tagtccaggg ccttctgtaa ccgctatggc
     8881 atcaatatct tccattgata cgtcggcatc cactagagcc tcgtttattg ttgttgttat
     8941 accttcaacg tgatgtctac ttgccacttc gggaacgaca ccgccaaatc gtttatgact
     9001 ttcaatctga cttaaaactg tatttgataa aatatctctg ccatttttta taacactaac
     9061 gcttgtttca tcacaacttg tttcaacagc tagtattaat atatctttag tcatttaaat
     9121 tcacccacat aaccattgcg tcctcacctt caccataata atttttacgt ttaccaccat
     9181 attgaaatcc taaattttca tatacatgtt gtgccacttt attattaact cttacttcta
     9241 aactcatcac atcacaagtg tgacttgcat agtttattcc gtattttaaa agcatttgac
     9301 ctaaaccata tcctctataa ttatcatcga ttgcaactgt cgtaatttga gcttgatcga
     9361 taacaatcca taaacctaaa taaccaataa tttgttgttc aaattctaag acaaaatatt
     9421 tcgcaaagtt attttgctct atttcatgat aaaatgcgtc aattgtccaa gaactatcat
     9481 tgaaactccg acgctcaata tcaaagactt gtggcacatc ttctttggtc atctctctaa
     9541 tgtttaattg ttcttttgac tgttgatcca atttcgttcc gcctcagcta atttatggta
     9601 tttaggagta aatgtatgta cgtctgaagg tttatctatc aattgataca tgactgatgc
     9661 atttggtagc tgcgcaatca catcaccttg taaatcatct tgtaatttta ctgtatcttt
     9721 cccaatataa ataaatggtt ggtttaaatc ttctaaaaaa gctcgcaatg catctatcga
     9781 catatattga tcttctaaaa tagtcactaa ttgaccattt tgccattgga atatgcctgt
     9841 ataaactgct tgtcgtcttg catcaaacac aggaaccaat aatttatcag tatgatcgat
     9901 tgttgctgcc aatgccttta atgatgaaac accatataat ttaacatcta acgcatacgc
     9961 taatgtttta gcaacagtaa caccgatacg taagccagta tatgaaccag gaccttcagc
    10021 aacaataatc gcatctaatt gctgtttagc tattttgctc tgctcaaata gctgactaat
    10081 cgcaggcatt aattgcactg aatgattttg tttagaatcc gtagttattt cagctaaaac
    10141 ttcatcgttt tgcatcaatg ctactgataa tggttgattc gatgtatcaa tgagcagcga
    10201 attcatggat aattgcctcc ttaatttgtt cataatgttc tccttgcgcg aacaactcaa
    10261 tttgtcttga attttcagat attgttgaaa tgttaataga taaatgcgtc gctgggagta
    10321 aatcttttat aaattgactc cattcaataa cagtaattgc ctgatcttcg aaaaattcat
    10381 caaaccctaa atcttcatca gaatcttcta agcgataaca atccatatga tgcaatttta
    10441 aattttttcc cctatatgat ttgatgatgt taaatgtcgg ggaattaatc gtacgtctta
    10501 caccaagagc ttttcctata aattgtgtta acgttgtttt acctgctcct aaatctccgt
    10561 taagtaaaat caaatcacca cttttcaatt gctcaactaa aaacatagca aattgattca
    10621 tttcatctaa attatttatc tttatcaatg ttgattctcc tatattatgc ttttcattca
    10681 taaaaatgat tatccattgt tcaatcgtat ctaactttat atttaacctt tatattgtaa
    10741 caaatttcaa cttaaatttc ttatctttga aacagattat ctcttcaaag ttaattgtaa
    10801 gaaaatttaa aatatttgtt gacatactaa agcagatata gtaaattaaa tttatcaaat
    10861 ttttagacaa ttctaactat taaagtggta tataccattc acggaaggag tataataaaa
    10921 tgcttaatca atatactgaa catcaaccga caacttcaaa tattattatt ttattatact
    10981 ctttaggact cgaacgttag taaatattta ctaaacgctt taagtcctat ttctgtttga
    11041 atgggacttg taaacgtccc aataatattg ggacgttttt ttatgtttta tctttcaatt
    11101 acttattttt attactataa aacatgatta atcattaaaa tttacggggg aatttactat
    11161 gcgaagcgac atgatcaaaa aaggagatca ccaagcacca gcaagaagtc ttttacatgc
    11221 cacgggcgcg cttaaaagtc caactgatat gaacaaacca tttgtagcta tttgtaactc
    11281 ttatattgat atcgttccag gacatgttca tttaagagag cttgcagata tagctaaaga
    11341 agcaattaga gaagccggtg ccattccatt tgaattcaat acaattggtg ttgatgatgg
    11401 aatagctatg ggacatatcg gaatgcgata ttctctacca tcacgtgaaa ttattgcaga
    11461 tgcagctgaa actgtaatta acgctcattg gtttgacggc gtattttaca ttcctaattg
    11521 tgacaagatt acacccggta tgattttagc agccatgagg acaaacgtac cagctatctt
    11581 ttgctctggt ggaccaatga aagctggctt atctgcacat ggtaaagcat taacactttc
    11641 atcaatgttt gaagcagtcg gcgcttttaa agaaggatcg atttctaaag aagaattttt
    11701 agatatggaa caaaatgcct gccctacttg tggttcatgt gctgggatgt ttactgcaaa
    11761 ttcaatgaac tgtttgatgg aagttttagg tctagcatta ccatacaacg gtactgcact
    11821 tgcagtcagt gatcagcgac gcgaaatgat tcgccaagca gcttttaaat tagttgaaaa
    11881 tattaaaaat gatttaaaac cacgtgatat cgttactcgc gaagcaattg atgatgcatt
    11941 tgcacttgat atggctatgg gtggttcaac aaacacggta ctgcatacgt tagccattgc
    12001 caatgaagct ggtattgatt atgacttaga gcgcattaat gctattgcca aacgcacgcc
    12061 atatttatca aaaatagcac ctagttcatc gtattcaatg catgatgtgc atgaagctgg
    12121 tggcgtccca gcaattatta atgaattgat gaagaaagat ggcacgttac acccagatag
    12181 aatcacagtt actggcaaaa cgttacgtga aaataacgaa ggcaaagaaa ttaagaactt
    12241 tgatgtcatt cactctcttg atgcaccata tgatgcacaa ggcggtttat ctatcttatt
    12301 tggtaatatc gcccctaaag gcgcagttat taaagttggc ggcgttgatc catctatcaa
    12361 aacatttact gggaaagcaa tttgtttcaa ttcgcatgat gaagctgttg aagcaataga
    12421 caatcgtacc gttcgtgcag gccacgtcgt tgtcattaga tatgaaggac ctaaaggtgg
    12481 accaggtatg cctgaaatgt tagcacctac ttcctctatt gttggtcgtg gcttaggtaa
    12541 agatgttgca ttaattactg atggtcgttt ttccggtgcc acaagaggta ttgcagttgg
    12601 tcatatttcc cctgaagctg catctggtgg accaattgcc ttaattgaag atggagatga
    12661 gattactatt gatttaacaa atcgtacatt aaaggtaaac cagcctgaag atgttctggc
    12721 gcgtcgccga gaatctttaa caccatttaa agcgaaagta aaaacaggtt atctagctcg
    12781 ttatactgcc ctagtaacta gcgcaaatac aggtggcgtc atgcaagtcc ctgagaattt
    12841 aatttaattt atttttatat tggagatggt taaaatgtct aaaactcaac atgaagtaaa
    12901 ccaaaatatt gaccctttaa aaatggctga atcacttgaa cctgaacaac taaatgaaaa
    12961 aactttaaat gacatgcgtt caggatcaga agtgctagta gaagctctac ttaaagaaaa
    13021 tgtggattat ttattcggtt atcctggtgg tgccgtacta cctttatacg acacatttta
    13081 tgatggtaaa atcaaacata ttttagcaat acacgaacaa ggtgctgttc atgctgcaga
    13141 aggttatgca cgtgtatctg gtaaaacagg cgtcgttgta gttacaagcg gtccaggtgc
    13201 aactaatgta atgacaggta ttacggatgc acattgcgac tctttaccac tagttgtatt
    13261 cactggacaa gttgctacac caggcattgg taaagatgca ttccaagaag cggatattct
    13321 atctatgact tcaccaatta caaaacaaaa ttatcaagtg aaacgtgttg aagatatccc
    13381 taaaatcgta cacgaagctt tccatgtagc taattctgga cgcaaaggtc ctgtagtgat
    13441 tgattttcca aaagatatgg gtgttttagc tacaaatgtg gatttatgcg acgaaatcaa
    13501 tattccaggt tatgaagttg ttacagaacc agaaaataaa gacattgaca ctttcatctc
    13561 acttttaaaa gaagcgaaaa agcctgtcgt attagccggc gcaggtatta atcaatcaaa
    13621 atcaaatcaa ttattaacac agtttgttaa taaacatcaa atcccaacag ttactacttt
    13681 actaggtcta ggtgctgtac cttatgagga tacactattt ttaggtatgg gaggtatgca
    13741 tggttcttat gctagtaaca tggcattaac tgagtgtgat ttactcatta atttaggtag
    13801 ccgcttcgat gatagattag caagcaaacc tgatgccttt gcacctaacg ccaaaattgt
    13861 acatgtagat attgatcctt cagaaatcaa taaagttatt catgtagatt taggtattat
    13921 tgcagactgt aaaagatttt tagaatgttt aaatgataaa aatgttaaga ctatagaaca
    13981 cagtgactgg gttaaacatt gtcaaaataa taagcagaaa catccattta aacttggtga
    14041 agaagatcaa gtattttgta agccacaaca aacaatcgaa tatatcggca aaattacaaa
    14101 tggtgaagca attgttacta cagacgtggg acaacatcaa atgtgggcag ctcaatttta
    14161 tccatttaaa aatcacggac aatgggttac aagcggtggt ttaggaacaa tgggattcgg
    14221 tattccttcg tcaattggtg ccaaattagc taatcctgat aaaacagtcg tatgcttcgt
    14281 cggtgacggt ggtttccaaa tgacaaacca agaaatggca ctattacccg aatatggttt
    14341 agatgtcaaa atcgtactaa tcaataatgg aacattaggt atggttaaac aatggcaaga
    14401 taagttcttt aatcaacgct tctcacactc agtatttaat ggtcaacctg attttatgaa
    14461 aatggcagaa gcatatggcg tcaaaggttt cttaatcgat aagccagaac aactggaaga
    14521 acaattagat acagcgtttg cttatcaagg gccagcttta attgaggttc gtatttcccc
    14581 tactgaagct gtaaacccaa tggttccaag tggcaaatca aatcatgaaa tggagggctt
    14641 ataatgacaa gaattcttaa attacaagtt gcggatcaag tcagcacgct aaatcgaatt
    14701 acaagtgctt ttgttcgcct acaatataat atcgatacat tacatgttac acactctgaa
    14761 caacctggga tttctaacat ggaaattcaa gtcgatattc aagatgatac atcacttcat
    14821 attttaatta aaaaattaaa acaacaaatt aatgttttaa cggttgaatg ctacgacctt
    14881 gttgataacg aagcttaatt ttaagacaaa ggcaatgatg cgctaattag ttatagatat
    14941 atcataggct gctagttaac atctgccact attacaaagc tatatttcag aattttcgaa
    15001 aaacaaaaca tttaattatt tggaggaatt tattatgaca acagtttatt atgatcaaga
    15061 tgtaaaaacg gacgctttac aaggcaaaaa aattgcagta gtaggttatg gatcacaagg
    15121 ccacgcgcat gcacaaaact taaaagacaa tggatatgat gtagtcatcg gtattcgccc
    15181 aggtcgctct tttgacaaag ctaaagaaga tggatttgat gtgttccctg ttgcagaagc
    15241 agttaagcaa gctgatgtaa ttatggtgct attacctgat gaaattcaag gtgatgtata
    15301 caaaaacgaa attgaaccaa atttagaaaa acataatgcg cttgcatttg ctcatggctt
    15361 taacattcat tttggtgtta ttcaaccacc agctgatgtt gatgtatttt tagtagctcc
    15421 taaaggaccg ggtcatttag ttagacgtac atttgttgaa ggttctgctg taccatcact
    15481 atttggtatt caacaagacg cttcaggtca agcacgtaat attgctttaa gttacgcaaa
    15541 aggtattggt gcaactcgtg caggtgttat tgaaacaaca tttaaagaag aaactgagac
    15601 agatttattt ggtgaacaag cagtactttg cggtggtgta tcgaaattaa ttcaaagtgg
    15661 ctttgaaaca ttagtagaag caggttatca accagaatta gcttattttg aagtattaca
    15721 tgaaatgaaa ttaatcgttg atttgatgta tgaaggcggt atggaaaatg tacgttactc
    15781 aatttcaaat actgctgaat ttggtgacta tgtttcagga ccacgtgtta tcacaccaga
    15841 tgttaaagaa aatatgaaag ctgtattaac tgatatccaa aatggtaact tcagtaatcg
    15901 ctttatcgaa gacaataaaa atggattcaa agaattttat aaattacgcg aagaacaaca
    15961 tggtcatcaa attgaaaaag ttggtcgtga attacgcgaa atgatgcctt ttataaaatc
    16021 taaaagcatt gaaaaataag atagacctac aatgaggagt tgttaaatat gagtagtcat
    16081 attcaaattt ttgatacgac actaagagac ggtgaacaaa caccaggagt gaattttact
    16141 tttgatgaac gcttgcgtat tgcattgcaa ttagaaaaat ggggtgtaga tgttattgaa
    16201 gctggatttc ctgcttcaag tacaggtagc tttaaatctg ttcaagcaat tgcacaaaca
    16261 ttaacaacaa cggctgtatg tggtttagct agatgtaaaa aatctgacat cgatgctgta
    16321 tatgaagcaa caaaagatgc agcgaagccg gtcgtgcatg tttttatagc aacatcacct
    16381 attcatcttg aacataaact taaaatgtct caagaagacg ttttagcatc tattaaagaa
    16441 catgtcacat acgcgaaaca attatttgat gttgttcaat tttcacctga agatgcaacg
    16501 cgtactgaat taccattctt agtgaaatgt gtacaaactg cagttgacgc tggagctaca
    16561 gttattaata ttcctgatac agtcggctac agttaccatg atgaatatgc acatattttc
    16621 aaaaccttaa cagaatctgt aacatcttca aatgaaatta tttatagtgc tcattgccat
    16681 gacgatttag gaatggctgt ttcaaatagt ttagctgcga ttgaaggcgg tgcgagacga
    16741 attgaaggca ctgtaaatgg tattggtgaa cgagcaggta atgcagcact tgaagaagtc
    16801 gcgcttgcac tatacattcg aaatgatcat tatggtgctc aaactgccct taatctcgaa
    16861 gaaactaaaa aaacatcgga tttaatttca agatatgcag gtattcgagt gcctagaaat
    16921 aaagcaattg ttggccaaaa tgcatttagt catgaatcag gtattcacca agatggcgta
    16981 ttaaaacatc gtgaaacata tgaaattatg acacctcaac ttgttggtgt aagcacgact
    17041 gaacttccat taggaaaatt atctggtaaa cacgccttct cagagaagtt aaaagcatta
    17101 ggttatgaca ttgataaaga agcgcaaata gatttattta aacaattcaa ggccattgcg
    17161 gacaaaaaga aatctgtttc agatagagat attcatgcga ttattcaagg ttctgagcat
    17221 gagcatcaag cactttataa attggaaaca ctgcaactac aatatgtctc tagcggcctt
    17281 caaagtgctg ttgttgttat taaagataaa gagggtcata tttaccagga ttcaagtatt
    17341 ggtactggtt caatcgtagc aatttacaat gcagttgatc gtattttcca aaaagaaaca
    17401 gaattaattg attatcgtat taattctgtc actgaaggta ctgatgccca agcagaagta
    17461 catgtaaatt tattgattga aggtaagact gtcaatggct ttggtattga tcatgatatt
    17521 ttacaagcct catgtaaggc atacgtagaa gcacatgcta aatttgcagc tgaaaatgtt
    17581 gaaaaggtag gtaattaatt atgacttata acattgttgc cctacctggt gatggaatcg
    17641 gtccagaaat tttgaacgga tctctatcat tgcttgaaat tataagtaat aaatataatt
    17701 ttaattatca aatagagcac cacgaatttg gtggtgcctc tattgataca ttcggcgagc
    17761 ctttaactga gaaaacctta aatgcgtgta aaagagcaga tgctatttta ctgggtgcaa
    17821 tcggtggacc taaatggaca gatcctaaca atcgaccaga acaaggatta ttaaaattac
    17881 gtaaatcatt aaatttattt gcaaatatac gtcccactac cgttgtcaaa ggcgctagtt
    17941 ctttatcacc tttaaaggca gaacgcgttg aaggcacaga tttagttata gtccgtgaat
    18001 tgacaagtgg tatttatttt ggagaaccta gatattttaa taatcacgag gccttagatt
    18061 ctcttactta tacaagagaa gaaatagaac gcattgttca cgtagcattt aaattggccg
    18121 cttcaagacg aggaaaacta acatcagttg ataaagaaaa tgtattagct tctagtaaat
    18181 tgtggcgcaa agtcgtaaat gaagtgagtc aattatatcc agaagtaaca gtgaatcact
    18241 tacttgttga tgcttgtagt atgcatttaa tcacaaatcc aaaacaattt gacgtcatcg
    18301 tttgcgaaaa cttatttggc gatattttaa gtgatgaagc ttcagtgatt cctggttcac
    18361 ttggtttatc accttctgct agttttagta acgatggtcc aagattgtat gagcctattc
    18421 atggatcagc accagatatt gcaggtaaaa acgttgccaa tccatttgga atgattctat
    18481 ctttagcgat gtgtttacgt gaaagcttaa atcaaccaga tgctgcagat gaattagaac
    18541 aacatattta taacatgata gaacatgggc aaacgacagc agatttaggc ggcaaactga
    18601 atactactga tattttcgaa attctatctc aaaaattgaa tcactaaggg ggagatgtaa
    18661 atgggtcaaa cattatttga caaggtgtgg aacagacatg tgttatacgg aaaattaggc
    18721 gaaccgcaac tattatacat tgatttacac cttatacatg aagttacttc tcctcaagca
    18781 tttgaatgac ttaggcttca aaacagaaaa ttaagacgcc cagatttaac atttgcaaca
    18841 ctcgatcaca atgttcctac tattgatatt ttcaatatta aagatgaaat tgcaaacaaa
    18901 caaatcacaa cattacaaaa aaacgccata gattttgggg tgcatatttt tgatatgggt
    18961 tctgatgaac aaggtattgt tcacatggtg ggacctgaga caggacttac acagcctggc
    19021 aaaacaatcg tttgtggtga ctctcacaca gcaacacatg gagcatttgg tgctattgca
    19081 tttggaattg gaacaagtga agttgaacat gttttcgcaa ctcaaacgct atggcaaaca
    19141 aaacccaaaa acttaaaaat cgatattaat ggcaccttac caacaggcgt ctatgctaag
    19201 gacattattc tgcatttaat taaaacgtat ggtgttgatt ttggtacagg ctatgctttg
    19261 gaatttactg gtgaaacaat taaaaacctt tcaatggacg gtcgaatgac tatttgtaac
    19321 atggctatcg aaggtggtgc caaatacggc ataatccaac ctgatgatat aacatttgaa
    19381 tatgttaaag gtcgaccatt tgccgataac ttcgctaaat cagttgataa gtggcgtgag
    19441 ctatattcta atgacgacgc gatatttgat cgtgtaattg aacttgatgt ttcaacatta
    19501 gaaccacaag tgacatgggg aactaatcct gaaatgggtg ttaatttcag tgaaccattc
    19561 cctgaaatca atgatatcaa cgatcaacgt gcgtatgatt atatggggtt agagccagga
    19621 caaaaagctg aagatatcga cttagggtat gttttcctcg gttcatgtac aaatgctaga
    19681 ctatcagatt tgattgaagc tagtcatatt gttaaaggaa ataaagttca cccaaatatt
    19741 acagctatcg tcgtaccagg ttctcgtaca gtaaaaaaag aagctgaaaa attaggtcta
    19801 gatactatct ttaaaaatgc aggatttgaa tggcgtgagc caggatgttc aatgtgttta
    19861 ggcatgaatc ctgaccaagt acctgagggc gtacattgtg catctacaag taatcgaaac
    19921 tttgaaggac gacaaggcaa aggtgcaaga acacatttag tatcccctgc tatggcagca
    19981 gcagcagcta ttcatggtaa atttgtggac gtaagaaagg tggttgttta aatggcagca
    20041 atcaaaccta ttacaacata taaaggtaaa atagtccctc ttttcaacga caatatcgat
    20101 acagaccaaa tcattcctaa agtacactta aagcgtattt caaaaagtgg ctttggtcca
    20161 tttgcttttg atgaatggcg gtacttacct gatggttcag ataatcctga tttcaatcca
    20221 aataagcctc aatataaagg tgcatccata ttaattactg gagataattt tggatgtggt
    20281 tcaagtcgtg aacatgcagc ttgggcactt aaggactatg gctttcatat tataattgca
    20341 ggaagtttca gtgacatttt ttatatgaat tgtacaaaaa atgcaatgtt accaattgtt
    20401 ttagaaaaaa atgcacgtga acatcttgct aaatatgttg aaattgaggt cgatttacca
    20461 aatcaaactg tgtcatcacc agacaagagt ttccattttg aaattgatga aacttggaag
    20521 aataaacttg taaatggctt agatgacatt gcaatcaccc tacaatatga atcattaata
    20581 gaaaaatatg aaaaatcact ttaagggagt tgaatattat gacagtcaaa acaacagttt
    20641 ctacgaaaga tatcgatgaa gcatttttaa gacttaaaga tattgtcaaa gaaacacctt
    20701 tacaattaga ccattactta tctcaaaagt atgattgtaa agtttattta aaacgagaag
    20761 atttacaatg ggtacgttct tttaaattaa gaggtgctta caatgctatt tctgttttat
    20821 cagatgaagc taaaagtaaa ggtattacat gtgcgagtgc aggtaatcat gctcaaggtg
    20881 ttgcctatac agctaaaaaa cttaatttaa acgctgttat ctttatgcca gtcactacac
    20941 ctttacaaaa ggtaaatcaa gtaaaattct ttggaaatag taacgttgaa gttgtactaa
    21001 ctggtgatac atttgatcac tgtttagctg aagctttaac ttatacaagt gaacatcaaa
    21061 tgaactttat agatccattc aataatgttc atacaatttc tggacaaggt acgcttgcta
    21121 aagaaatgct agaacaagca aagtctgaca atgttaactt tgattatcta tttgccgcaa
    21181 ttggtggtgg tggtttaatt tcaggtatta gtacctattt taaatcctat tctcccaaca
    21241 caaaaattat cggagttgaa ccttcaggtg ctagtagtat gtacgaatct gttgtcgtaa
    21301 ataatcaagt tatcacatta cctaatatcg ataaatttgt cgatggagct tctgtagcca
    21361 gagttggcga tattactttt gaaattgcca aaaaaaatgt agataactac gtacaagtag
    21421 atgaaggtgc tgtatgttca acaattttgg atatgtattc aaaacaagca atagtagcag
    21481 aacccgctgg cgctttaagt gtaagtgcgc ttgaaaacta taaagatcat attaaaggta
    21541 aaacagtggt ttgtgtcatt agtggtggta ataatgatat taatcgaatg aaagaaattg
    21601 aagaacgttc attactatat gaagaaatga agcattactt tatcttaaat ttccctcaac
    21661 gtccaggtgc attgagagaa tttgtaaatg acgtattagg acctcaagac gatattacta
    21721 aatttgaata cttaaaaaaa tcttctcaaa atacaggtac tgtcattatt ggtattcaac
    21781 ttaaagatca tgatgattta atacaactca aacaacgtgt aaatcatttc gatccttcca
    21841 atatttatat taatgaaaat aagatgttat attcattgtt aatttaacac atattaagaa
    21901 aagcagtcat aaattgattt ctaattgaaa tcatcttatg actgcttttt attatgcttt
    21961 acattattga ggttgcacgc tttgtatacc atcgtatatt tctttgagcc taaagtcttc
    22021 ttcattcccc accccattat ttatattttg aatggcagtt gaaattactt tgtcataaat
    22081 agccttctca ctttctggta aattatgtcg tggtatactc atcaaagcat atgctttttg
    22141 tttatcttca atattttcta tacgattctt attattatta aaattctcat tagcttcact
    22201 accattatta ttaatattat gcgtgttttc tttttcatgt ttgttttctt gcatatgatt
    22261 cgggatttct gacttatttt cagcatctgt aggaaaatat attttataag caaaaaatcc
    22321 acacacaatc aatgagcata caaataaaaa agttaacatc gtaccaataa atattttcaa
    22381 atccgttctc ctttcaacca ttcattctta catgtatatt tagtattaaa aaatccttat
    22441 agtctcaact tgcctggcaa cgttctactc tagcggaacg taagttggct accatcgtcg
    22501 ctaaaaacct ttcttgactt gtgacaatcg cttgcttctt tcctctcctt cggctctcgc
    22561 ttactcattt agctctacta aactcgttgc gctcttttct cgtttcgtca gattcaaacg
    22621 ttttcacttc gccaagccat ttttctttgt gttaactttt attttgacgt tttagacata
    22681 aaaaaagaga ccttgcggtc tcaacttgcc tggcaacgtt ctactctagc ggaacgtaag
    22741 ttcgactacc atcgacgcta aggagcttaa cttctgtgtt cggcatggga acaggtgtga
    22801 cctccttgct atagtcacca gacatatgaa tgtagattat acattcaaaa
//
LOCUS       DATTQF010000026        22733 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.026, whole genome shotgun sequence.
ACCESSION   DATTQF010000026 DATTQF010000000
VERSION     DATTQF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.026"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            177..578
                     /locus_tag="VB241_000934"
     CDS             177..578
                     /locus_tag="VB241_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1244239.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(697..1260)
                     /locus_tag="VB241_000935"
     CDS             complement(697..1260)
                     /locus_tag="VB241_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1244240.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLVKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINALLSLMSTEGIIVLVIYHGHSEGQLEKQALLDYLSTLEQ
                     KHAQVLKYQFLNQRNHAPFICAIEKIS"
     gene            complement(1257..2210)
                     /locus_tag="VB241_000936"
     CDS             complement(1257..2210)
                     /locus_tag="VB241_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEP1244241.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMQTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2321..3502
                     /locus_tag="VB241_000937"
     CDS             2321..3502
                     /locus_tag="VB241_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1244242.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMVLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3791..6208
                     /locus_tag="VB241_000938"
     CDS             3791..6208
                     /locus_tag="VB241_000938"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP1244243.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNSEGKVEVFTTRPDTIYGASFLVLSPEHALVDSITTDEYK
                     DQVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEKKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6230..6541
                     /locus_tag="VB241_000939"
     CDS             6230..6541
                     /locus_tag="VB241_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP1244244.1"
                     /translation="MKSITTDELKTKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNAFNKKETYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            6865..13434
                     /gene="sasC"
                     /locus_tag="VB241_000940"
     CDS             6865..13434
                     /gene="sasC"
                     /locus_tag="VB241_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HEP1244245.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDHNV
                     QGGSNQALPGNSQNTNADTNRDIVNDSQNTPNAHATDNTSTNQALTNHQNVDVANQVG
                     PAPIQPSASPAQNNNNSNANSTATEPAANTNNNLASNNNTLNVPNNTDNNDSARHLTL
                     KEIQEDVRHSSDKPELVAIAEEASNRPKKRSRRAAPTDPNATPADPTATPADPTAGNG
                     SAPVAITAPYTPTTDPNANNIGQNAPNEVLSFDDNNIRPSTNRSVPTVTVVDNLPGYT
                     LINGGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIRGNDTNDHGDFNGIEKTL
                     TVNPNSELIFEFNTMTTKNYQGMTNLIIKNADNDTVIGEKVVAYGPIWRLLKVPENVS
                     HLKIQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNK
                     EFTVTTSLKNNGNFGASFNTDDFVYKIQLPEGVEYVNNSLTKDFPSGNSGVDINDMNV
                     TYDAANRIITIKSTGGGTGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKT
                     YSQDFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRR
                     AQTILDENRNNVPLNKRVSQADIDSLANQMQHTLIRSVDAENAVNRKVDDMEDLVNQN
                     DELTDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPN
                     AKQAIRDKAAKQREIINHTPDATQDEIQDALNQLTTDETDAIDNVTNATTNADVETAK
                     NNGINTIGAVAPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATN
                     HALEQINQATTNDDVDTAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANP
                     DATQEERQAAIEKVNAAVAVANTNILNANTNADVEQVKTNAIQGIQAIEPATKVKTDA
                     KNAIDQSAETQHNAIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKD
                     QGTQNIVVIQPATQVKTDARNAVNEKAREAITNINATPGATREEKQEAINRVNTLKNR
                     ALNDIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLTDLATAKKQEINQNTN
                     ATTEEKQVALNQVDQDLATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAAL
                     AQTNQHYSAKLVEINATPDATDDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATV
                     AENNIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQA
                     FNQINQNQTNDQVDATTNQAINAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDS
                     TDNEKEVALQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKIKPAARE
                     KINQKANELRAQINQDKEATAEERQAALDKINDLVAKAMTNITNDRTNQQVNDSTNQA
                     LDDIALVTPDHIVRAAARDAVKQQYEAKKHEIEQAEHATDEEKQVALNQLANNEKRAL
                     QNINQAIANNDVKRVESNGIATLKGVEPHIVVKPEAQEAIKASADNQVESIKDTPHAT
                     TDELDEANQQINDTLKQGQQDIDNTTQDAAVNDVRNQTIKAIEQIKPKVRRKRAALDN
                     IDESNNNQLDAIRNTLDTTQDERNVAIAALNKIVNAIKNDIAQNKTNAEVDQTEADGN
                     NNIKVILPKVQVKPAARQSVSAKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAID
                     QINHADKTAQVNQNSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATD
                     EEQNAAIVQVEKELIKAKQQIAGAVTNADVAYLLHDGKNEIREIEPVINKKATAREQL
                     TTLFNDKKQAIEANVQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTATLNLQ
                     TIHDLDVHPIKKPDAEKTINDDLARVTHLVQNYRKVSDRNKADALKAITALKLQMDEE
                     LKTARTNADVDAVLKRFNVALGDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQL
                     AKVKALIDQYVADGNRMVDEDATLNDIKKDTQLIIDEILAIKLPAEVIKASPKVGQPA
                     PKVCTPIKKEDKQEVRKVVKELPNTGSEEMDLPLKELALITGAALLARRRSKKEKES"
     gene            complement(13551..14819)
                     /locus_tag="VB241_000941"
     CDS             complement(13551..14819)
                     /locus_tag="VB241_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1244246.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQNDNVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIIDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIENQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDTFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAEIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            15223..16884
                     /locus_tag="VB241_000942"
     CDS             15223..16884
                     /locus_tag="VB241_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP1244247.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVAIKLALNYPLIMLFHTPGAVLSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVEAVFFIANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            16881..17576
                     /locus_tag="VB241_000943"
     CDS             16881..17576
                     /locus_tag="VB241_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP1244248.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEEDIQAFKEGITLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            17593..18015
                     /locus_tag="VB241_000944"
     CDS             17593..18015
                     /locus_tag="VB241_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP1244249.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            18221..19630
                     /gene="sapep"
                     /locus_tag="VB241_000945"
     CDS             18221..19630
                     /gene="sapep"
                     /locus_tag="VB241_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HEP1244250.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEVGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAKAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            19634..20482
                     /gene="dat"
                     /locus_tag="VB241_000946"
     CDS             19634..20482
                     /gene="dat"
                     /locus_tag="VB241_000946"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEP1244251.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            21033..21824
                     /locus_tag="VB241_000947"
     CDS             21033..21824
                     /locus_tag="VB241_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP1244252.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            21839..22483
                     /gene="trmB"
                     /locus_tag="VB241_000948"
     CDS             21839..22483
                     /gene="trmB"
                     /locus_tag="VB241_000948"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP1244253.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGEMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKNGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
BASE COUNT         8249 a         3400 c         4234 g         6850 t
ORIGIN      
        1 ttggttttta tacttattta attttattca acttagacaa ttgaatagaa agcaagttta
       61 tttacacttg tagttttatg cataagttag cacatataag ttttgggatt gttgggatgt
      121 ttgttaatac ttgtatggta gctaaatatg tgattattaa ttgggagatg tatagcatga
      181 aaaaagtaaa taacgatact gtatttggga ttttgcaatt agaaacactt ttgggtgaca
      241 ttaactcaat tttcagcgag attgaaagcg agtacaaaat gtctagagaa gaaattttaa
      301 ttttactaac tttatggcaa aaaggttcta tgacgcttaa agaaatggac agatttgttg
      361 aagttaaacc gtataagcgt actagaacgt ataataattt agttgaatta gaatggattt
      421 acaaagagcg tcctgttgac gatgaaagaa cagttattat tcatttcaat gaaaagttac
      481 aacaagagaa agtagagttg ttgaatttca tcagtgatgc gattgcaagt agagcaacag
      541 caatgcaaaa tagtttaaac gcaattattg ctgtgtaagt ttaatagcat aaaaagaggt
      601 tttcattaag ttgaaaacct ctttttgtcg ttgatatttt tttcaaatgt tagctactca
      661 atcctaaatt ataaaaaagt atagcgcagc aaatgtttaa gaaatttttt ctatggcaca
      721 aatgaatgga gcatgattac gttggtttaa aaattgatat ttcaaaactt gcgcatgctt
      781 ttgctccaaa gtactcaaat aatcaagcaa agcttgcttt tcaagttggc cttcactatg
      841 accatgatag ataactaata cgataatacc ttcggttgac attaaagata ataacgcatt
      901 aatagcttgg attgtcgtgt caggctttgt cacgatagat ttatcacctt taggcaaata
      961 acctaggtta aaaatggctg catcaatatg acctttatgt gcatcattta tatgatgttc
     1021 aatattttca tgtccatctt ttactaaaga aacatgattg aaatccttaa ctttatcacg
     1081 tgtattttcc aaagctaaat cttgaatgtc gaaaccataa acatgtcctt ctggtacttg
     1141 ttcggctaaa aataaagtgt cattgccgtt accgcaagtt gcgtctacaa caatactttc
     1201 tggtgttata tgttgtttaa taagtgtttt tgaaaagggg agtattcgtt ctaatttcat
     1261 tgcttcacct tagacttgta acgcaagcct tgataagaat ttctacgtgc taattcagcg
     1321 tcgatgccat ttaatacttc ccatttatta acactccaca ttggaccgac catgatatct
     1381 attggaccat caccggtaat tcggtgaacg atcatttcag ggggaatcac ttctaattgg
     1441 tctacaacta ggtttgtgta ttcttcttga gtcataaaag ttaataaacc tttatcatat
     1501 tgttttacca tcggtgtacc ttttaacaaa tgaagtaaat gaattttaat accttgtaca
     1561 tccatttgtg caacttcttt ggcagtagcc atcatcatgt catagtcttc gccaggtaag
     1621 ccattaatga tgtgtgtaca tacattgata ttatgtttac gtaattttgc cacgccatca
     1681 taataagttt gcatatcatg ggcacgattg attaaatcag atgttgattg atggattgtt
     1741 tgtagtccca attcaaccca taaatatgtt cgttgattca aatctgctaa atattcgaca
     1801 acatcgtctg gtagacagtc aggacgcgta ccaatagata atcccacaac acccggttct
     1861 ttaagtacag gttcgaattt ttcttttaac acttcaactg gtgcatgtgt atttgtaaat
     1921 gcctgaaaat aagcaatata ttttccttcg tgccatttct catgcatctt ttccttaatt
     1981 tctttaaatt gtactgcaat tgaatctgca cgattacctg caaagtctcc actacctgca
     2041 gcagaacaaa atgtacatcc accatgtgct acagtgccat cacgattagg acagtcaaac
     2101 ccgccatcca atgcaacttt aaatattttt tgtccaaatt tattttttaa atggtaattc
     2161 catgtgtgat aacgtttgtt ttcaaaagcg tattggaaat gattgcccat aagtcatttt
     2221 cctttctata aaaaaaagag ttctaagtac agattttaac atattttaat gttatagtgt
     2281 ttattatagt ttgacaaaaa agagagagga actatgaaat atgaatatac ctaaatcagt
     2341 ctggtggcta gtaattggca tggcgttaaa tattactggt tccagttttt tgtggccttt
     2401 aaatacaatt tatatgaaac aagaacttgg aaaaagttta actgttgctg gtttagtgct
     2461 aatgataaat tcatttggca tggttattgg aaacttatta ggtggttcac tatttgataa
     2521 attaggtgga tacaagacga ttttaattgg aactttcact tgtctttgta gtacaacgct
     2581 acttaatttc tttcacgggt ggccttggta tgctgtatgg cttgtaatgt taggctttgg
     2641 tggcggaatg attattcctg cgatatacgc aatggctgga gcagtgtggc cgaatggcgg
     2701 aagacaaacg tttaatgcga tatacttagc gcaaaatatt ggtgttgctg tcggcgctgc
     2761 aatgggcggc tttgtcgcag aatttagctt taactatatc tttttagcca atcttattat
     2821 gtatgttgtg tttgcacttg tcgcggtaac gcaatttaat attgaaatta atgcgaaagt
     2881 taaatatcca actcatttag atattactgg taaaaagaat aaagcaagat ttatttcatt
     2941 agtactaatt tgtgcaatgt ttgcaatttg ttgggttgcg tatattcaat gggagtctac
     3001 aatcgcatca tttacacaat cgattaatat ttcaatggca caatatagtg ttttatggac
     3061 aattaacgga ataatgattt tagtagcaca accattaatt aaaccaattc tctatctgtt
     3121 aaaaggaaac ttaaagaagc aaatgtttgt cggcatcatc atttttatgt tgtcgttctt
     3181 tgtcacgagt tttgccgaaa actttacaat atttgttgtc ggtatgatta ttttaacttt
     3241 tggagaaatg tttgtatggc ctgcagttcc aactatagcc aatcagttag cgccagatgg
     3301 taagcaagga cagtaccaag gttttgtgaa ttcagctgct acagtaggaa aagcattcgg
     3361 tccatttctt ggtggtgtat tagttgatgc atttaatatg cgcatgatgt ttatcggtat
     3421 gatggtacta cttgtatttg cattaatatt attaatggtt ttcaaggaga ataatacgca
     3481 acctaaaaaa atagatgcat aatgagtaaa tagaattaac gttatagact tgaaataaat
     3541 gtcgttataa cataatatta atttgtataa tttaatttcg tttggagctt ttctacagaa
     3601 agctagtgat gctgagagct agtgttaagg actaaatgta aatcgtatta attttaaatt
     3661 gaatgaatga catctcttac tattaaaatg agtgcacaat ttttgtgaaa tagggtggta
     3721 acgcggcaaa tgtcgtccct atgtaaatag aatagttaga ggtgtctttt tttattgaat
     3781 aggaggaaat gtgttgaatt acaaccacaa tcaaattgaa aagaaatggc aagactattg
     3841 ggacgaaaat aaaacattta aaacaaatga taacttaggt caaaagaaat tttatgcttt
     3901 agacatgttt ccatacccat caggtgctgg tttacatgtt ggacatcctg aaggctatac
     3961 agcaacagat atcatttcaa gatataagag aatgcaagga tataatgtat tacatccgat
     4021 ggggtgggat gcattcggat taccagcaga gcaatatgct ttagatactg gtaacgaccc
     4081 acgtgaattt acaaagaaaa atatccaaac ttttaaacga caaattaaag aattagggtt
     4141 cagttatgat tgggatcgtg aagttaatac aacagatcca gaatactata aatggacaca
     4201 gtggattttc atacagttat ataacaaagg tttagcatac gttgacgaag ttgcagttaa
     4261 ctggtgtcct gcattaggta ctgtcttatc taacgaagaa gtgattgacg gtgtatctga
     4321 acgtggcggt cacccagttt atcgtaaacc aatgaaacaa tgggtactta aaattacaga
     4381 atatgcagat caattattag cagatttaga tgatttagat tggcctgagt ctttaaaaga
     4441 tatgcaacgt aactggattg gacgttcaga aggtgccaaa gtttcatttg atgtagataa
     4501 ttcggaagga aaagtagaag tatttacgac tagaccagat acaatctatg gtgcatcatt
     4561 cttagtctta agtcctgagc atgcattggt tgattcaatt acaactgatg aatataaaga
     4621 tcaagtgaaa gcgtatcaaa cagaagcttc taaaaagtct gatttagaac gtacagattt
     4681 agcaaaagat aaatcaggtg tgtttactgg tgcatatgca attaatcctt tatctggtga
     4741 aaaagtacaa atttggattg ctgattatgt attatcaaca tatggtactg gtgcaattat
     4801 ggcagtacca gcgcatgatg acagagatta tgaatttgct aaaaagtttg atttgccaat
     4861 cattgaagtc attgaaggtg gaaatgttga agaagcggca tacactggtg aaggtaaaca
     4921 tattaattct ggtgaacttg atggtttaga aaatgaagcg gcaattacta aagctattca
     4981 attattagag aaaaaaggtg ctggcgaaaa gaaagttaat tacaaattaa gagattggtt
     5041 attcagtcgt cagcgttatt ggggcgaacc aattcctgtc attcattggg aagatggaac
     5101 aatgacaact gttcctgaag aagagctacc attgttgtta cctgaaacag atgaaatcaa
     5161 gccatcaggg actggtgagt caccactagc taatattgat tcatttgtaa atgttgtaga
     5221 tgaaaaaaca ggtatgaaag ggcgtcgtga aacaaataca atgccacaat gggcaggtag
     5281 ttgttggtat tatttacgtt acatcgatcc taaaaatgaa aatatgttag cagatcctga
     5341 aaaattaaaa cattggttac ctgttgattt atatatcggt ggcgtagaac atgcagtgct
     5401 tcacttattg tatgcaagat tttggcataa agtactttat gatttaggta tcgtacctac
     5461 aaaagaacct ttccaaaaat tatttaacca aggtatgatt ttaggagaag gtaatgagaa
     5521 gatgagtaaa tctaaaggaa atgtaatcaa tcctgatgat atagtacaat ctcatggggc
     5581 agatacttta cgtctttacg aaatgtttat gggaccttta gatgctgcaa ttgcatggag
     5641 tgaaaaagga ttagatgggt ctcgtcgatt cttagatcgt gtatggcgtt taatggtaaa
     5701 tgaagatgga acattaagtt caaaaatagt aactacaaat aataaatctt tagataaagt
     5761 ttataaccaa actgttaaaa aggtaacaga agactttgaa acattaggat ttaatactgc
     5821 tattagtcaa ttaatggtat ttattaatga gtgttataaa gttgatgaag tttataaacc
     5881 ttacattgaa ggcttcgtta aaatgttagc acctattgca ccacatatcg gtgaagaatt
     5941 atggtcaaaa ttaggacatg aagagtctat tacgtaccaa ccttggccaa cttatgacga
     6001 agcactactt gtagatgatg aagtagaaat cgttgttcaa gttaatggta aattgagagc
     6061 taaaattaaa attgctaaag atacatcaaa agaagaaatg caagaaattg ccttatctaa
     6121 tgacaatgtt aaagcgagta ttgaaggtaa agacatcatg aaagtcatcg ctgttcctca
     6181 aaaattagtc aatattgtag ctaaataatg ttataaggag gactttaaaa tgaagtcaat
     6241 tactacagat gaattaaaaa ccaaactttt agaatctaaa ccagttcaaa ttgttgatgt
     6301 tcgtactgac gaagaaacag caatgggata tatacctaat gcaaagttaa ttccaatgga
     6361 taccattccc gacaatttaa atgcatttaa caaaaaagag acatactata ttgtatgtgc
     6421 tggtggagtt cgaagcgcta aagttgtaga atatttagag gcaaatggca ttgatgccgt
     6481 aaatgtcgaa ggcggcatgc acgcttgggg cgatgaaggt ttggaaataa aaagtattta
     6541 aagtagtgac ataatttaaa ataatattac atttgtaatg acaccaagta acgtttcggt
     6601 tgcttggtgt tttttgctat gaattgcttt ctgttacaaa acaatctaaa gcgttcttgt
     6661 tatgattttt taagtgatta attacaaaac ggaaactaaa ttgtaataaa ataaaacttt
     6721 attttataaa atgatgatga taaaattgag tgaacttaaa atgttgtaca aaataatatg
     6781 gttataaaca taatcgggga gcttatattt ttagcataat tctaaacaac acagcagaga
     6841 atagacaacc aggaggaaaa cgaaatgaat ttgttaaaga aaaataaata tagtattaga
     6901 aaatataaag tagggatatt ctctacttta atcgggacag ttttattact ttcaaaccca
     6961 aatggtgcac aagctttaac tacggatcat aatgtgcaag gtggttcaaa tcaagcatta
     7021 cctggcaact cacaaaatac aaatgccgat actaatcgag acatagtaaa tgattcgcaa
     7081 aatactccta atgcacatgc aacagacaat acatcaacaa atcaagcatt gactaatcat
     7141 caaaacgttg atgtggcaaa tcaagtcggg cctgctccaa tacagcctag cgcgtcgcct
     7201 gcgcaaaata ataataattc taatgctaat tcaacagcaa cagagccagc ggcgaataca
     7261 aataataatt tagcatcaaa taacaataca ttaaacgtgc ctaataatac agataacaat
     7321 gattcagcgc gtcatctgac tttaaaagaa attcaagaag atgttcgtca ttcgtctgat
     7381 aagccagagt tagttgcgat tgctgaagaa gcatctaata gaccgaaaaa gagaagcaga
     7441 cgtgctgcgc caacagatcc taatgcaaca ccagcagatc caacggctac accagcagat
     7501 ccaacggcag gaaacggtag tgcaccagtt gcaattacag cgccatacac gccaacaact
     7561 gatcccaatg ccaataatat aggacaaaat gcacctaacg aagtgctttc atttgatgat
     7621 aacaacatta gaccaagtac gaaccgttct gtgcctacag taactgttgt tgataattta
     7681 ccaggctaca cactgattaa tggtggtaaa gtaggggtgt ttagtcatgc aatggtaaga
     7741 acgagcatgt ttgattcagg agatgccaag aactatcaag cgcaaggcaa tgtaattgca
     7801 ttgggtcgta ttagaggaaa tgatacaaat gatcatggcg attttaatgg tatcgagaaa
     7861 acattaacag taaatccgaa ttctgaatta atctttgaat ttaatactat gactactaaa
     7921 aactatcaag gtatgacaaa tttaatcatt aaaaatgctg ataacgatac tgttattggt
     7981 gaaaaagtag ttgcttatgg tccgatttgg cgcttattaa aagtacctga aaatgttagt
     8041 catctaaaaa ttcaatttgt acctaaaaat gacgcaataa cagatgcacg tggtatttat
     8101 caattacgag atggatataa atactatgac tttgtagact caatcggtct tcattctggg
     8161 tcacatgtct atgttgaaag acgtacaatg gagccaacag caacaaataa taaagaattt
     8221 acagttacaa cgtcattaaa gaataatggt aactttggcg cttcattcaa tacagatgat
     8281 tttgtatata aaattcaatt acctgaaggt gttgaatatg taaataattc attgactaaa
     8341 gattttccta gcggtaattc aggtgttgat attaatgata tgaatgtgac gtatgacgca
     8401 gcaaatcgaa ttattacaat taaaagtact ggtggaggta cagggaattc gccggcacga
     8461 ctaatgcctg ataaaatatt ggatttgaag tataagctac gtgtgaacaa tgtgccaaca
     8521 ccaagaacag taacatttaa cgatacatta acgtataaaa catattcaca agattttatt
     8581 aattcacctg ctgaaagtca tactgtaagt acaaatccat atacaattga tatcatcatg
     8641 aataaagacg cattgcaagc cgaagtcgat agacgaattc aacaagcgga ttatacattt
     8701 gcatcattag atatttttaa tgatcttaaa agacgcgcac aaacaatttt agatgaaaac
     8761 cgtaacaatg tacctttaaa caaaagagtt tctcaagcag atatcgattc attagcaaat
     8821 cagatgcaac atacgttaat tcgcagtgtt gacgctgaaa atgccgttaa tagaaaagtt
     8881 gatgacatgg aagatttagt taaccaaaat gatgaactga cagatgaaga aaaacaagca
     8941 gcgattcaag tcatcgagga acataaaaat gaaattattg ggaatattgg tgaccaaacg
     9001 actgatgatg gcgttactag aattaaagat caaggtatac agactttaag tggagacact
     9061 gcaacaccag ttgttaaacc aaatgctaaa caagctatac gtgataaagc agcgaaacaa
     9121 agagaaatta tcaatcacac gccagatgct actcaagatg aaattcaaga tgcattaaat
     9181 caattaacaa cggatgaaac agatgctatt gataatgtta cgaatgctac taccaatgct
     9241 gatgttgaaa cagctaaaaa taatggtatt aatacaattg gtgcagttgc gccacaagtg
     9301 acacacaaac aagctgcaag agatgcaatt aatcaagcga cagcaacgaa acgacaacaa
     9361 ataaatagca atagagaagc aacacaagaa gagaaaaatg cagcattgaa tgaattaacg
     9421 caagccacga accacgcatt agaacaaatc aatcaagcga caaccaatga tgatgtagat
     9481 actgccaaag gtgatggtct gaatgccatt aatcctattg cgcctgtaac tgttgtcaag
     9541 caagcagcaa gagatgccgt atcacatgat gcacaacagc atatcgcaga gatcaatgca
     9601 aatcctgatg cgactcaaga agaaagacaa gcagcaatag agaaagtaaa tgctgctgta
     9661 gctgttgcga atactaatat attaaatgct aataccaatg ctgatgttga gcaagtaaag
     9721 acaaatgcaa ttcaaggtat acaagccatt gaaccagcta caaaggttaa aacagatgct
     9781 aaaaacgcta ttgatcaaag tgcggaaacg caacataatg cgatatttaa taataatgat
     9841 gcgaccttag aagagcaaca agcagcacaa caattgcttg atcaagctgt agccacagcg
     9901 aagcaaaata ttaatgcagc agatacgaat caagaagttg cacaagcaaa agatcagggc
     9961 acacaaaata tagttgtgat tcaaccggca acacaagtta aaacggatgc acgcaatgct
    10021 gtaaatgaaa aagcgcgaga ggcgataaca aatatcaatg ctacacctgg cgcgactcga
    10081 gaagagaaac aagaagcgat aaatcgtgtc aatacactta aaaatagagc attaaatgat
    10141 attggtgtga cgtctactac tgcgatggtc aatagtatta gagacgatgc agtcaatcaa
    10201 atcggtgcag ttcaaccgca tgtaacgaag aaacaaactg ctacaggtgt attaacggac
    10261 ttagcaactg caaaaaaaca agaaattaat caaaatacaa atgcaaccac tgaagaaaag
    10321 caagtagcat taaatcaagt agaccaagat ttagcaacgg caattaataa tataaatcaa
    10381 gctgatacta atgcagaagt agatcaagca caacaattag gtacaaaagc aattaatgcg
    10441 attcagccaa atattgtaaa aaaacctgca gcattagcac aaaccaatca gcattatagt
    10501 gctaaattag ttgaaatcaa tgctacacca gatgcaacag atgatgagaa aaatgctgcg
    10561 atcaatactt taaatcaaga cagacaacaa gctattgaaa gtattaaaca agcaaataca
    10621 aatgcggaag tagaccaagc tgcgacagtg gcagagaata atatcgatgc tgttcaagtt
    10681 gacgttgtaa aaaaacaagc agcgcgagat aaaatcactg ctgaagtagc gaagcgtatt
    10741 gaagcggtta aacaaacacc taatgcaact gacgaagaaa agcaggctgc agttaatcaa
    10801 atcaatcaac ttaaagatca agcgtttaat caaattaatc aaaaccaaac aaatgatcag
    10861 gtagacgcaa ctacaaatca agcgattaat gctatagata atgttgaagc tgaagtagta
    10921 attaaaccaa aggcaattgc agatattgaa aaagctgtta aagaaaagca acagcaaatt
    10981 gataatagtc ttgattcaac agataatgag aaagaagttg ctttacaagc attagctaaa
    11041 gaaaaagaaa aagcacttgc agctattgac caagctcaaa cgaatagtca ggtgaatcaa
    11101 gcggcaacaa atggtgtatc agcgattaaa attattcaac ctgaaacaaa aattaaacca
    11161 gcagcacgtg aaaaaatcaa tcaaaaagcg aatgaattac gtgcgcaaat taatcaagat
    11221 aaagaagcga cagcagaaga aagacaagcg gcgttagata aaatcaatga tttagttgct
    11281 aaagctatga caaatatcac gaatgataga acaaatcagc aagttaatga ctcaacaaat
    11341 caagcgcttg acgacattgc attagtgacg cctgaccata ttgttagagc agctgctaga
    11401 gatgcagtta agcaacaata tgaagctaaa aagcacgaaa ttgagcaagc ggaacatgcg
    11461 actgatgaag aaaaacaagt tgctttaaat caattagcga ataatgaaaa acgtgcatta
    11521 caaaacatta atcaagcaat agcgaataat gatgtgaaac gtgttgaatc aaatggtatt
    11581 gctacgttaa aaggcgtaga accgcacatt gtggttaaac ctgaagctca agaagccata
    11641 aaagcgagcg cagataacca agtagaatct ataaaagata caccacatgc tacgacagat
    11701 gaattagatg aagcaaacca acaaataaac gacacactta aacaaggtca acaagatata
    11761 gacaatacga cacaagatgc agctgtcaat gatgttagaa accaaacgat taaggcaatc
    11821 gaacaaatta aaccgaaagt tagacgcaaa cgtgcagcgt tggataacat tgatgaaagt
    11881 aataataatc aactcgatgc aatacgaaat acgctagata caacgcaaga tgaacgaaat
    11941 gttgctattg ctgcgttaaa taaaattgtt aatgcaatta aaaatgatat tgcacaaaac
    12001 aaaacgaatg cagaagtgga tcaaactgag gctgatggta acaacaacat caaagtgatt
    12061 ttacctaaag ttcaagttaa accagcagcg cgtcaatctg tcagcgcaaa agctgaagct
    12121 caaaatgcac ttattgatca aagtgattta tctaccgaag aagaaagatt agctgctaaa
    12181 catttagtag aacaagcact taatcaagct attgatcaga tcaatcacgc agataagact
    12241 gcgcaagtta atcaaaatag tatcgatgct caaaatatta tttcaaaaat taaaccagcg
    12301 acaacagtta aagcaacagc attacaacaa attcaaaata tcgctacaaa taaaattaat
    12361 ttaattaaag caaataacga agcgacagat gaagaacaaa atgctgcaat agtacaagtt
    12421 gaaaaagagt taattaaagc taaacaacaa attgctggtg cagtgactaa tgctgatgtg
    12481 gcatatttat tgcatgatgg gaaaaacgaa attcgtgaaa tcgaacctgt tattaataaa
    12541 aaagcaactg cgcgagaaca attaacaaca ttattcaacg ataagaaaca agcaattgaa
    12601 gcgaatgttc aagcaacagt agaagaaaga aatagtattt tagcacagtt acaaaacatt
    12661 tatgacactg ctattggaca aattgatcaa gatcgtagca atgcacaagt tgataaaaca
    12721 gcaacattaa atctacaaac aatacatgat ttagacgtac atcctattaa aaagccagat
    12781 gctgaaaaaa cgattaatga tgatcttgca cgtgttacac atttagtgca aaattatcga
    12841 aaagtaagtg atcgtaataa ggctgatgca ttaaaagcta taactgcatt aaaattacaa
    12901 atggatgaag aattaaaaac agcacgcact aatgctgatg ttgatgcagt tttaaaacga
    12961 tttaatgttg cattaggcga tatagaagca gtaattactg aaaaagaaaa tagcttactg
    13021 cgcattgata acattgctca acaaacatat gcgaaattca aagcgatcgc aacaccagaa
    13081 caattagcta aagtaaaagc attaattgat caatatgttg cagatggcaa tagaatggtt
    13141 gatgaagatg cgacattaaa tgacatcaaa aaagatacgc aactcattat tgatgaaatt
    13201 ttagcaatta aattacctgc tgaagtgata aaagcgtcac caaaagtggg gcaacctgct
    13261 ccaaaagttt gtacgcctat taaaaaagaa gataaacaag aagtgcgaaa agttgtaaaa
    13321 gaacttccaa atactggttc tgaagaaatg gatttaccat taaaagaatt agcactaatt
    13381 acaggcgcag cattattagc tagaagacgt tctaaaaaag aaaaagaatc ataataaaca
    13441 aaaagacctt aatataacga cactttttag tgactcagtt atattaaggt catttttaat
    13501 tgaatgaaaa ggttgatcta ctagagagaa tcgaacccaa ttttatacta ttattccatt
    13561 gatgcatgtg agtaatgacc ggcatataat ccagcgacat gtcctgttac gagtgcactt
    13621 gtaatattat aaccaccagt ataaccatgt atgtctaata cttcaccaca taaaaataat
    13681 ccaggaacta atttagacat catggtttta ggttgaattt cttttagtga cacaccacca
    13741 cctgtgacaa atgccttatc tataggtaat gtcccgttta ccttaaatac aaacccttta
    13801 aacatattta ctaagtcgtt caattgttga tttgataagt gatgtgatgt ggtattttca
    13861 tcgatttcag cttgttccag catgaacaat aagtagcgct cttcaattag tccatgcaag
    13921 ctgtttttaa tgattttatc tggtgtgtcc gataataatg atgtgatgtg ttgttttaat
    13981 tgttcatggt ttaattcagg aaatgtatcg attgccattg aaatatgctg tgtcttttga
    14041 tttttttgtt ctttataaac aaactgacta catcttaatg cagctggacc actgatacca
    14101 aaatgagtaa ataacatatc catttgatga ctgatgcgtt ttttaccatt tttcttaagt
    14161 actgacaatt caacatcttt taaacttaag ccttttagac gattggattt aatgaaaggt
    14221 tcagctgatg taattggaac ttcggtcggg aataactccg taatggtatg acctaaatct
    14281 tgtgcaaact tataaccatc acctgttgaa ccagtttgag ggacacttgt accacctgta
    14341 gcaattacta gcgaatggct ttcataacta ttattttgag tatgtacagt gaaagtttgg
    14401 tctgtattaa cttcgattct actaacagct tcttcttctt taatcgttac atgttgattt
    14461 tctatagttg tcactaaagt atcaacgacg tcttgtgcct tattggaaac tggaaacata
    14521 cgaccgtgat cttcttcttt taatttaaca cccctagact caaaaaaatc tatgatggat
    14581 tcattatcaa taattgaaaa gggactatat aaaaatttcc catttccagg aatgttctta
    14641 ataatttcag catagggtaa tcgattagtt acgttacatc taccgccacc agatattttg
    14701 agtttacgac ctagaccttt ctttttttcg atgagtagaa cgttgtcatt ttgttcgctt
    14761 gcagctactg ccgccattaa gccgctaggt ccgcctccga taataattgt ttgatacata
    14821 tacgcttcct ccaaatttgt tactggacat tcaattgcta ttttatagta cttttttaaa
    14881 ctttgctata tctcttgtgt tatgaaatga cactcgcgct gaatatattt ataatgtgtg
    14941 tttcataaaa ggataagcat ttattcgaac gctattatta ttttaagagt tgtccgccat
    15001 ttattggcat tagtagtata atgacaatat gaaaatttat gaatttcaaa gcaactttga
    15061 gtattcagtg acaacacgtt attatttttg ttatgatatt tcattgaata tacgacaatg
    15121 aaataaagtt ttgtgatata ttcataataa tgaaaatgat tttacagaga aatagctttt
    15181 tatttacaaa acattaataa acatgagata gggagatacg taatgagtga aagtaaagaa
    15241 atggtgcgtg ggaccttttt aattacaatt agtattttga ttacaaaggt gttaggcgta
    15301 ctttttatca ttccattcaa ctatttaatt ggtggacaag aaaatatggc gccgttcaca
    15361 tacgcttatg caccatataa tattgcaatt gcagttgcga cagcaggtgt accattagct
    15421 gcttctaaat atgtagcgaa gtataatgcg attggtgcat ataaagtcag tcagaaattt
    15481 tataaatcta gttttattgt catgagtatt accggtgttt taggtttctt agtactttat
    15541 ttcctagcac catatatttc tgaattaact ttagctagaa atgttcacga taaaaatggt
    15601 tggtcagttg atgacattac atggattatt agaatcatta gtatggtagt catcttcatt
    15661 cctgtattag caacatggag aggtatattc caaggttata aatctatggg acctactgcg
    15721 gtatcggaag taactgagca aattgcacgc gtgattttca tattaatcgg aagttattta
    15781 gtactaaatg tctttgatgg ttctatctta ttagccaatg gtattgctac ttttgcagca
    15841 gcagttggtg cgattattgg tatattcaca ctctggtatt attggagaaa gcgtaagcat
    15901 aatattgatc gtatggtcga atctgattac acagatatag atgtgtcata tggcaagatg
    15961 tataaagaaa ttattgctta tagtattccg ttcgttattg taagtttgaa ttatccatta
    16021 tttaacttag tagatcaatt tacacataac ggtgctttat ctttagttgg tataccttca
    16081 cagttacaag atatattctt taatatgcta aatatgtcaa ctaataaaat tgtaatgatt
    16141 ccgacatcat taagtgctgg ctttgcagta agtttaattc cttatattac aaaaacattt
    16201 gcagaaggtc gcttacatga aatgcatcat caaattagaa catctattgg tgtattaatg
    16261 tttataactg ttccagctag tattggaatt atggccttgg cacaaccgtt attcacagta
    16321 ttctatggtt acgatccaat tgttttaggt catgacccta accatgatgg tagtcgtttg
    16381 ctattctatt atgcgcctgt agcaatatta atttcactat taagtgtaac ggcgtcaatg
    16441 ttacaaggta tcgataagca gaagttaacg gtttatgtta ttttagcttc tgttgcaatt
    16501 aaattagcat taaactaccc attaattatg ctattccata cgccgggggc tgttttaagc
    16561 acaagtatcg cattattatt cgcaattggt tgtaacttct atattcttaa aaaatacgcg
    16621 aaatttaaat ttagctatag ctggattcat ttcgcaaaaa tattcttata ctcatttatt
    16681 atgatgttag gtgtagaagc agtattcttc attgcgaacc tattcttaga gccaacaaaa
    16741 cttggatatt taattattat cattttaggt gtaacagttg gtattctaat ttacggtaca
    16801 atcacaatta aaacgcgtct tgcggacgaa ttcttaggtg aaattcctga aaaattaaga
    16861 cgtagagtta ggtttttacg atgagaatag ataaattttt agcaaatatg ggcgtcggaa
    16921 cacgtaatga ggtcaaacag ttacttaaaa aaggccttgt aaatgtcaac gaacaagtaa
    16981 ttaaatcacc aaaaacacat atagaaccag aaaacgataa gatatcagtt cgtggagaat
    17041 taatagaata cgttgaaaat gtctatatta tgttaaataa gccaaaaggt tatatatcgg
    17101 caactgaaga tcatcattca aaaaccgtta ttgatttaat acctgaatac caacatttaa
    17161 atatttttcc ggttggtcgt cttgataaag atacagaagg gcttttattg ataacgaatg
    17221 atggtgattt taatcatgaa ttaatgagtc caaacaaaca tgtttctaaa aaatatgaag
    17281 ttatttcagc aaatcctatc actgaagaag acattcaagc ttttaaagag ggtataacac
    17341 tgtctgatgg caaggttaag cctgcaatat taacttatat tgataatcaa acgtcacacg
    17401 taacaattta tgaaggaaaa tatcatcaag ttaaacgaat gtttcatagc attcaaaatg
    17461 aggtattaca tttaagacgt atcaaaattg ctgatttaga attggatagc aatttagatt
    17521 caggagaata tcgtttatta actgaaaatg attttgacaa attaaattat aaataaaagg
    17581 gagacgattg atatggcaaa aggtaattta tttaaagcga ttttaggtat aggtggcgct
    17641 gtagcagctg tacttgttac acgtaaagat agtcgtgaca agctgaaagc agaatataat
    17701 aaatacaaac aagatcctca aagctataaa gataatgcta aggataaagc gacgcaatta
    17761 ggaacaattg caaatgaaac aattaaagaa gtaaaaacaa atccgaaaga atatgctaat
    17821 agattaaaaa ataatccaaa agcatttttc gaagaagaaa aatcaaaatt taccgaatat
    17881 gacaataaga ctgacgaaag tattgaaaaa ggtaaatttg atgatgaagg tggcgcagca
    17941 ccaaataata atttacgtat cgtcactgaa gaagatttaa aaaagaataa aaatgcattg
    18001 tctgataaag aataaggcat acaattaata agttggtatt gaactggcat attaaattaa
    18061 attaatacag aaaaggaaca aagcagttat cattaatttt atgttaaatc gatagcgttg
    18121 ttttgttcct ttttattatt ttctgacatc ttattgtagt ttttatatca tttcatgtaa
    18181 aataggattt aacttataga aaaaaggaag tcgatttcga atgtggaaag aaaaagttca
    18241 acaatacgaa gatcaaatca ttaatgactt aaaaggatta ttagcaattg aaagtgtgag
    18301 agatgatgca aaagcatcag aagacgcacc agttggtcca ggtcctcgta aggcattaga
    18361 ctacatgtat gaaattgcac atagagatgg atttacaaca catgatgtgg atcatattgc
    18421 aggaagaatt gaggtaggta aaggaaatga cgtattaggt atcttatgtc atgttgacgt
    18481 tgttcctgct ggtgatggat gggatagtaa tccgtttgag ccggttgtaa cagaagatgc
    18541 tatcatagct agaggtacac ttgatgacaa aggtccaaca attgctgctt attatgcaat
    18601 taagatatta gaagatatga atgtggattg gaagaaacgt attcatatga ttattggtac
    18661 ggatgaagaa tctgattgga aatgtacgga tcgctatttt aaaacagaag aaatgccaac
    18721 attaggtttt gcaccagatg cagaatttcc gtgtattcat ggtgaaaaag gcattacaac
    18781 atttgactta gttcaaaata agcttgctga agatcaagat gaacctgatt atgaattaat
    18841 aacttttaaa tctggtgaac gttacaacat ggtacctgat catgcagaag caagagtgct
    18901 tgttaaagaa aatatgacag atgttattca agactttgag tattttttag aacaaaatca
    18961 tttacaaggt gatagtactg ttgatagtgg cattctagtt ttaacagttg aaggtaaagc
    19021 ggttcatggt atggacccat ctatcggtgt gaatgcgggt ctttacttac taaaattctt
    19081 agcatcatta aatcttgata ataatgcaaa agcgtttgta gcatttagta atcgctactt
    19141 atttaattca gattttggtg aaaagatggg aatgaaattc catacagatg tcatgggtga
    19201 tgtgacaact aacattggtg ttattacata tgataatgaa aatgcgggtc ttttcggtat
    19261 caacttgcgc tacccagaag gatttgaatt tgaaaaagct atggatcgtt ttgcaaatga
    19321 gattcaacaa tatggctttg aagtgaaatt aggtaaagtc caaccaccac attatgttga
    19381 taaaaatgat ccttttgtac aaaagttagt tactgcatat agaaatcaaa caaatgatat
    19441 gactgaacct tatactatag gtggcggtac ttatgcgaga aacttagaca agggtgtagc
    19501 atttggcgca atgtttagtg attctgaaga tttaatgcat cagaaaaatg aatatatcac
    19561 taaaaaacag ttatttaacg caactagtat ttacttagaa gcaatttatt cattatgcgt
    19621 ggaggaataa tatatggaaa aaattttttt aaatggtgag tttgtaagtc caagtgaagc
    19681 aaaggtttca tacaacgaca gaggatacgt atttggcgat ggtatttatg aatacattcg
    19741 agtatacaat ggtaagttat ttacagtaac agaacattat gaaagatttt tacgtagtgc
    19801 caatgagatt ggtttagatt taaattattc tgtagaagaa ttaattgaac tatctcgtaa
    19861 attagttgat atgaatcaaa ttgaaactgg ggcaatttat attcaagcaa cgcgaggtgt
    19921 agctgaaaga aatcatagct tcccgacacc tgaagtagaa ccagcaattg ttgcttatac
    19981 taagagttat gatcgtcctt atgatcattt agaaaatggt gtgaatggtg ttaccgttga
    20041 agatatccga tggttacgtt gcgacattaa aagcttgaac ctattaggaa atgtattagc
    20101 aaaagaatat gctgtgaaat ataatgcagt tgaagcaatc caacatcgag gcgaaactgt
    20161 cactgaagga tcttcaagta atgcttatgc aattaaagac ggtgtgattt atacacatcc
    20221 gattaacaac tatattctta atggtattac ccgaattgta attaaaaaaa ttgccgaaga
    20281 ctataacatc ccatttaaag aagaaacgtt tactgtagat ttcttgagaa acgcagatga
    20341 agttattgtt tcaagtactt cagctgaggt tacacctgtt attaaattag atggtgaacc
    20401 agttaatgat ggtaaagttg gcccaattac acgtcaatta caagaaggat ttgaaaagta
    20461 tatagagtca cacagtattt aaaattctat catcatattt ttagattaaa ttacttaaat
    20521 ttatcaactt tcgtaagtga attatgttat aatttaaaac gaagcttaaa agtgttgttt
    20581 agaaaacaca tattagtaaa aagtgccatt ttgtttctca ttttcagttg aaatataatg
    20641 ataaaaatta taaaattttc tatgagttac aaatatgatg tgcatcatat tttactaagt
    20701 acgtaaatgt tacagttcat aaatttatgt taaccatgaa aatgggatag caattatttt
    20761 gaaaactaga gtttatcgcc tacaaaagtt acaaaaacat gtagttttag acacggtaac
    20821 attttatgtc aagtaagtga ctatagaatg tatggtgtca atgacagaca ttacgattta
    20881 aatttagtcg tgtaatgtag gcattggttg aggatttaaa ataattatct ggacataaaa
    20941 tttgtaccag tatggtgtta taaattagaa tatatggctc ttgaaaaacc tatttctaga
    21001 gccattttct aattgaaagt gaggtggacg agttggagca gttttatcaa ttagggtgga
    21061 cacttgattc tgcaggtggt gcatctggtg aagcatatat ggctgaacaa gatggacaaa
    21121 agttgttttt aaaacgaaat tcaaatccat ttattgcggc attatcagca gaaggtattg
    21181 tgcccaaatt agtatggacg aaacgcatag aaacaggcga ggttgttaca gcgcaacatt
    21241 ggaaaaatgg ccgtgaacta tcttcaaatg aaatgaagca aacaagagtt gcacatttat
    21301 taaagaagat acacaattct agacctttat tgagtatgtt aaagcgtatg gaaatggaac
    21361 ctattactcc tgagattatg cttaataaaa ttaatgcctc tttatcaaga gaagttttaa
    21421 cacatcatat tgtgagaaaa tcattaacct atttagaaga gcatataccg agtttagatt
    21481 cgcgtttttt cactgttgta catggtgatg tgaatcataa taattggtta ttatccgatc
    21541 gagatgaact atttttagtc gattgggaag gtgcaatgat tgcagacccg gcaattgata
    21601 tcggaatgct gctttacaac tatgttccac aacaacaatg gtctgaatgg cttgaaacat
    21661 atggagttca agagagcttg aatttaaata aacgtatgaa atggtatacg gtaattcaat
    21721 ctataggtct tgttcaatgg tatgaagagc aaaaaagata taaggatatg aacacttggc
    21781 ttaaattcct aaatgaagtt atgaatagta acatgtttat ttaagaggag aataaaatat
    21841 gagagttcga tacaaaccat gggctgaaga ttatttaaaa gatcatcctg aattggttga
    21901 catggacggt caacatgcag gggaaatgac tgaatggttt gataaaacgc aaccgataca
    21961 cattgaaatt ggttcgggta tggggcagtt tattacaaca ttagctgctc aaaatcctca
    22021 tattaactat atttcaatgg aacgtgagaa aagtatcgtt tataaagtat tggataaagt
    22081 taaagagatg ggtttaacaa atttaaagat tatttgtaat gatgccattg aattaaatga
    22141 atattttaaa aacggcgaag tttcgcgtat ttatttgaac ttttcagatc catggccaaa
    22201 aaatcgtcat gcaaaacgac gtttaacgta tcatacgttt ttagcgttgt atcaacaaat
    22261 cttaaatgat gaaggagatt tacactttaa aactgataac agaggactat ttgcctacag
    22321 tttagaaagt atgtctcaat ttggaatgta ttttacaaaa ataaatctaa atttacatca
    22381 agaagacgat ggcagtaata ttttaacaga atatgaaaag aaattttcgg ataaagggtc
    22441 acgtatttat cgtatggaag cgaaattcca ttcacaaaaa tagagcactt atgagggtat
    22501 gtacacatac aatcataagt gttttttcgt actataatgg ttctttagat aaagcatatt
    22561 ggttctgtaa taaacccgac agatggaatt ttttcacttt tcatatgtcc gcttttttga
    22621 ttttgaatat aaaaaggttc ttcacctttt atggttgtca ctttgcttga tatatgagaa
    22681 ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac gtt
//
LOCUS       DATTQF010000027        22299 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.027, whole genome shotgun sequence.
ACCESSION   DATTQF010000027 DATTQF010000000
VERSION     DATTQF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.027"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(248..409)
                     /locus_tag="VB241_000949"
                     /pseudo
     CDS             complement(248..409)
                     /locus_tag="VB241_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            653..1024
                     /locus_tag="VB241_000950"
     CDS             653..1024
                     /locus_tag="VB241_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1244254.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTEILEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            963..1217
                     /locus_tag="VB241_000951"
     CDS             963..1217
                     /locus_tag="VB241_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244255.1"
                     /translation="MVNVYLISKMAKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYKGWKRFPVQRLTGGD"
     gene            1218..2237
                     /locus_tag="VB241_000952"
     CDS             1218..2237
                     /locus_tag="VB241_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1244256.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            2253..3902
                     /locus_tag="VB241_000953"
     CDS             2253..3902
                     /locus_tag="VB241_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP1244257.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEDSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDISHGEVKLFNYDAKVNLKQLLPYEACVIELN"
     gene            complement(3985..4851)
                     /locus_tag="VB241_000954"
     CDS             complement(3985..4851)
                     /locus_tag="VB241_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1244258.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(5081..6565)
                     /locus_tag="VB241_000955"
     CDS             complement(5081..6565)
                     /locus_tag="VB241_000955"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEP1244259.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            6878..7798
                     /gene="rnz"
                     /locus_tag="VB241_000956"
     CDS             6878..7798
                     /gene="rnz"
                     /locus_tag="VB241_000956"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP1244260.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPIVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(7903..8718)
                     /gene="proC"
                     /locus_tag="VB241_000957"
     CDS             complement(7903..8718)
                     /gene="proC"
                     /locus_tag="VB241_000957"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP1244261.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            8860..9615
                     /locus_tag="VB241_000958"
     CDS             8860..9615
                     /locus_tag="VB241_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1244262.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     DQELGKTINVIPCDLNDLKSIQALQFEDNTLFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            9631..9879
                     /locus_tag="VB241_000959"
     CDS             9631..9879
                     /locus_tag="VB241_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244263.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILSDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQVITNNDELTCEVKYRKMF"
     gene            complement(10095..11003)
                     /locus_tag="VB241_000960"
     CDS             complement(10095..11003)
                     /locus_tag="VB241_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1244264.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALFKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNDDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            11085..11627
                     /locus_tag="VB241_000961"
     CDS             11085..11627
                     /locus_tag="VB241_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP1244265.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNVEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            11732..12181
                     /locus_tag="VB241_000962"
     CDS             11732..12181
                     /locus_tag="VB241_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEP1244266.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            12230..13117
                     /gene="xerD"
                     /locus_tag="VB241_000963"
     CDS             12230..13117
                     /gene="xerD"
                     /locus_tag="VB241_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP1244267.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(13195..13701)
                     /locus_tag="VB241_000964"
     CDS             complement(13195..13701)
                     /locus_tag="VB241_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEP1244268.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEKQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNHETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            13793..14524
                     /locus_tag="VB241_000965"
     CDS             13793..14524
                     /locus_tag="VB241_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP1244269.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDIDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTELSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIVRGVNYHFG"
     gene            14517..15059
                     /gene="scpB"
                     /locus_tag="VB241_000966"
     CDS             14517..15059
                     /gene="scpB"
                     /locus_tag="VB241_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEP1244270.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDISKDQLVELIENYSS
                     HGLIIQRFGTTYVLTTKKEASMYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            15052..15789
                     /locus_tag="VB241_000967"
     CDS             15052..15789
                     /locus_tag="VB241_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP1244271.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            15922..16647
                     /gene="srrA"
                     /locus_tag="VB241_000968"
     CDS             15922..16647
                     /gene="srrA"
                     /locus_tag="VB241_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HEP1244272.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            16628..18379
                     /gene="srrB"
                     /locus_tag="VB241_000969"
     CDS             16628..18379
                     /gene="srrB"
                     /locus_tag="VB241_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HEP1244273.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNTRFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWTYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            18631..19563
                     /locus_tag="VB241_000970"
     CDS             18631..19563
                     /locus_tag="VB241_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HEP1244274.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESSKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVH
                     CDDHDIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKQELDSLT
                     EVLKEYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDK
                     TFSDGMKNSKKQLKDKSRPYVVTTLFSTKDNYTKDNTIDEMIDFSEVLKKKKNIPHDL
                     NVSLQITDKYINSARPSYSKKDSIEVGVFNHEKN"
     gene            19550..21727
                     /locus_tag="VB241_000971"
     CDS             19550..21727
                     /locus_tag="VB241_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244275.1"
                     /translation="MKKINDRDLAELSGYWAYQDLDKRYVIRVNNNKYRLIEDYSDNP
                     NSKSFGGTDAKVFELVNSNNELTGQQAIIFQGTNNNKSIGSNNPFRGKVADDWIENIK
                     LMNDKNKSTSLLEQNNAYIKSYEQKLKDADTLKSSDFLRKYKKNPSTYKNKTIKSDGG
                     NSQGGASANHQGMINPNKNIVSTNPAMLPKSIWENFKSQNFENMINYHSKFDILSWLQ
                     DPFATPTLGKRVNLETGVPTMDGLVNSHLGYKRKLNPRYNTYKDLAVYKIKSVKDTMI
                     KNGKKVKKTIEIDINMDDRIPINVWTGDSIARTGKGTPIKLNLENLSALSNLVTGETS
                     NMLTDCVNYLNESYNISQVENANFGERKHKLKQDFKKIVEVDILEGMSRELTSLKKDA
                     FSAIDNVKKNLVGIAFIFSSVAVLIFELNTIEEMLEDLDKKLQRGIESLHDSLDNVIK
                     EMFKNLDHDFEDGVTEEMMKHLNVVNNNINFVKKQNDVYGEQITDIKNIMSHQDATVM
                     DGNLSINYSGEHMISGQVQSSNYLTRKMTILKNHIDNAVNKISDYVQEAYNNYFEPVL
                     NYILEVIHSVETAVRHIYEISSLIYKVTMKIKIKAMGINVDEIDKNLEVLKSKLKNLD
                     EFLNNLKTASPILENHLDDIVRNMKPLIVNQIFEPSHYDDMFILNTQAHARLDQMAQQ
                     FEVVCNGLNENEGQAIQTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            21817..>22299
                     /locus_tag="VB241_000972"
     CDS             21817..>22299
                     /locus_tag="VB241_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HEP1244276.1"
                     /translation="MLKKAKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFSLKVKATNVVGSGDGVEVYVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFFK
                     D"
BASE COUNT         8031 a         3122 c         4016 g         7130 t
ORIGIN      
        1 aaaatgttca acagagttca aatataatta atgacacagg tgcaggagat gttttttcag
       61 catcagtatt tcattattgg cttgaaaact atgaagttga taattactta gaaatggcat
      121 tgtttaatgc ctgttctaca gtgagcacta tatataatac ttccatgatt aattagcaac
      181 acatgtaaat ggcaactata aaaatggctg ctatgtcaaa ttggactgat gaattcaata
      241 taggaagtta ggcaactaaa cattgtataa ctttcttttt agttattgga gcgtaaagtt
      301 ttgaacataa taatattcga ttgcgcaaat gattgtaact tccataatca aatcatgtac
      361 gtttaattaa ttttattatg ttagttatac attctaatgg acaatgtgag ctacacccag
      421 ttaagttgac atagaaaata cgaaaacttt gaacgaagta tttcattgat atatgtccca
      481 gatgacatgt ttgattaatg aagttctatg tggctacaac atataaaaaa ttacacaagt
      541 attatctttg accttaaaag catcccgcca tatttttaag cattttgtga agtaataaaa
      601 tatttactgt gttatgatag catgtactat agagaaatag aggtactatt ttatggaaaa
      661 gttaaatcaa gttatgttat atgttgatga tgttgaaaaa gcgaaagcat tttggacaga
      721 aattttagaa tttgttgttg taagtgaaac accgttagct gaggactatg ttgcagttga
      781 agtatcacct acgaaagatg ctgaaacgtc attgacgatt atggcaaaag aatttattga
      841 aaagtatagc ccggaggtga acctaggaac accatcttta atgtttaaag agaaaaactt
      901 tgatgcatta tactcaaaat taaatgattt aggtttaact gggcatgata tagtagaaat
      961 gaatggtcaa cgtgtattta atttccaaga tggccaaggt aattattttg cagttagtga
     1021 ctaaacaatc gattttaata tttatacaaa cctttatatt gattttcgga ttgaatttca
     1081 gtatgaaggt tttattttta tcgttttatg caatcgtttg cacaaatctt ttgacttttt
     1141 tagaaagtgt gactaaactg gtattataca aaggttggaa gcgttttcca gtacagagat
     1201 taacaggagg tgattaaatg gttacgatta aagatgttgc actaaaagcc ggtgtttctc
     1261 cttcgacagt ttcaagagtt ataaaaggaa atcaacgtat aagcgaagcg acaatttcaa
     1321 aagtgaagaa agttatggaa gaattgaatt attttcctaa taccgctgct agaactttaa
     1381 ttactaacca aacatataaa attggtttag tgttaaaagg gtctgaggag cctattcgac
     1441 tgaatccatt ctacatcaat gtattgctag gaatttctga aacgtgtaac cagcatggct
     1501 atggtacaca aacgacagtc tcaaataata tgaatgattt aatggatgaa gtttataaaa
     1561 tgattaaaca acgaatggtt gatgcgttta tactgctcta ttcaaaagaa aatgatccga
     1621 ttaaacaaat gttaattgat gaaagtatgc catttattgt gattggtaag cctacatcgg
     1681 atatagatca tcaatttaca catatagata atgataatat attagcttct gaaaatttga
     1741 cacgacatgt tattgaacaa ggtgtagatg aattaatatt tattacagaa aaaggcgatt
     1801 ttgaagtttc gaaagacagg attcaaggat ttgaaacagt tgcatcacaa tttaatctgg
     1861 attatcaaat tattgagact agtaatgaaa gagaagttat ttttaattac atgcaaaatc
     1921 tgcatacgcg tttgaaggac ccaaatatta aacaggcaat catttcgtta gatgctatgt
     1981 tacatttagc gattttaagt gtcctatatg aacttaatat tgaaattccg aaagatgtaa
     2041 tgacagcaac gttcaatgat tcttatttaa ccgaaattgc gtcaccacct caaaccagta
     2101 ttgacatcaa gcctcgaatg ttaggtcaac aggctggtgc agcaatttta aatatattaa
     2161 aaaataaagc acaggatgtt attgaactcg tcattataga tacagaatta aaaataagaa
     2221 aatcaacaca gcgatagcaa aggagtttta aaatgaataa gcaatggtgg aaagaagcag
     2281 tagcatatca agtatatcca agaagtttta atgatagtaa tcacgatggt attggggatt
     2341 tacctggaat gattgataaa ttggactact taaaagattt aggtatcgat gtcatttggc
     2401 tcagtccaat gtttaaatca cctaatgatg ataatggtta tgatattagt gactaccaag
     2461 agattatgga tgaatttgga acgatggaag actttgatcg tttattaaaa ggtgttcatg
     2521 atagaggtat gaagcttatt ttagatttag ttgtgaatca tacatctgat gaacatcctt
     2581 ggtttataga atccaaatct agtaaagaca atcccaaacg tgattggtac atttggcaag
     2641 atccaaagcc agatggctct gaacctaaca actgggaaag tatatttaat ggatctacat
     2701 gggaatatga tgctaatact gagcaatatt atttccatct attcagtaaa aaacaacctg
     2761 atttgaattg gggtaatccg gaagttagag atgctgtatt tgaaatgatg aactggtggt
     2821 ttgataaagg cattgatgga tttagagtag atgcaattac gcatattaag aagacgtttg
     2881 aagcgggtga cttacctgta cctgagggta aaacatatgc cccagcattt gatgtagata
     2941 tgaatcagcc aggtatacaa acttggttac aagagatgaa agatcgctca ttaagtaagt
     3001 atgacattat gactgttggt gaagcgaatg gtgtaagccc tgatgatgct gatgactggg
     3061 tcggggaaga aaatggtaaa tttaatatga tattccaatt tgaacatttg ggactgtgga
     3121 atagtggtga ttctcacttt gatgtaaatt cgtataagtc tgtattaaat agatggcaaa
     3181 aacaacttga aaataaaggt tggaatgcgt tgtttattga aaatcatgac caaccacgac
     3241 gtgtatcgac gtggggtgac gatgacaagt attggtatga atcagcaaca agtcatgcag
     3301 cagtttattt cttgcaacaa ggtacgccat tcatttatca aggtcaagaa attggtatga
     3361 cgaattatcc atttgaaagt attgaaacat ttaacgatgt tgctgttaaa aatgactatc
     3421 aaattgtgaa agctcaaggt ggagatgtag acgctttact tgcgaaatat aaagatgaga
     3481 accgagataa ttctcgcaca ccaatgcaat gggatgatac gttaaatgga ggatttacaa
     3541 atggtgaacc gtggttccca gtgaatccga attataaaac tatcaatgtt gcacaacaat
     3601 tagaagatga ggattcagta ttacaatttt ataaagattt aattcaatta agaaagtcta
     3661 atgatgtata cgtatatggt caatttgatt tagtagatgc tgaaaattca caagtttttg
     3721 cgtacacgag aacattaaat gaaaagcaag ttcttattgt aggtaatctt actaaccacg
     3781 aagcagaatt aactgtacca tttgatataa gtcatggaga agtgaagcta tttaattatg
     3841 atgccaaagt taatttaaaa cagttacttc catatgaagc atgtgttatc gaactaaatt
     3901 aacatattaa tatgccccct aatagaatct aaggccattt gtagacctat agatttatta
     3961 gggggcttct ttgtttttaa ttatttaatc ttcgtcataa ggactctgtt taacaggaat
     4021 ccaaagctga attttagtga atgggtcatt aaatgaaata tcgagagggt acacttcaac
     4081 atataaatca tttcgttcat atggcaatgt taactgcaaa cttgattcaa tatagtacca
     4141 agcttcatta gttgcataat caatttcacc ttgtaaattg aatttcgcac aatgtttgcc
     4201 aggtaaaaat cgactttcta agtgtgcagg ataacgttca cttggtacac ctacaaatat
     4261 ttctaaacca ttttcaagag gacaactaat aacaaatagt tcaaatggac taacgtcatt
     4321 atatcgtcgt aactctttaa ttttaccatc aatgagcaag tcttctaaaa aatccggaac
     4381 attaaaagga tgtgacaaat acttagtgtc tataaatcgt gcatatccaa ccaatgaaat
     4441 atcatctgtc tcttctaatc tgtatggata aggtgctctc tcagtagttg ataatttgat
     4501 atataatcgc tcttgaattt gtaattcatc ttttttagta gaagcttgaa taggtgatac
     4561 gccgtgaaaa tcactaaaat catttgcaaa atcatttgaa tttgcatagt gatatttttt
     4621 agcgatatct acaagtcgtg tagcaccatt aatcacatca tttgcagcga gtgtcatttt
     4681 acgcgcgcgt gcataagctt ctggagataa gccgacaatc attttaaatg attgatcaag
     4741 atggtatggc gaaagaccaa cgtaatcact taattcttgc aaattgaaag gctctaataa
     4801 acgatcttca atataaacaa ttgcctgttg tatttgcttg ataacgtcca aaactttcac
     4861 tccaattaat tcaatcattt atttttattc tacattattt ctataaatta tacacccatt
     4921 tgttcaatga ttattaaaat agttttgggc attgtaaaat ataatttcat aatatagtct
     4981 agaaaaaaag cgaatgatag aacaattgat atacttgatt cgtaatcaat ccttgtcatt
     5041 cgctcattta tttttgttta acatgtgcgc tttaattcaa ttattgaata tcgtcccacc
     5101 aatggttacc atcacgagca agtaataaat cactttctaa tggaccatta gtacctgatt
     5161 catagttagg gaattctgga tcaaccatat tccattcatc ttgaattgca tcaacaaatt
     5221 tccatgttga ttttaattct tcccagtgcg taaagttagt ggcatcacct ttaagacaat
     5281 cgaataatag attttcatat gcatctacag tattcatttt atcttgagcg ctcattgaat
     5341 aagacaattg aacaggttca gtttcgatac cttgtgtatt tttcttagca tttagatgta
     5401 aagatacacc ttcatttggt tgaatattga ttactaatag gtttgaatct aacagtttat
     5461 cagtttcata gtataagttc attggtactt ctttaaattc aacgacaact tgaattgttt
     5521 tagatttcat acgtttacca gtacggatat agaatggtac gccagcccat ctaaagttat
     5581 caattgttaa tttacctgaa acaaatgtag gtgtgttaga gtcatctgca acgcgatctt
     5641 catcacggta tgctttaact tgtttaccat cgatatagcc ttcgccatat tgaccacgaa
     5701 caaagttctt tttaacatct tcagattgga aatgacgcag tgatttaagt acttttactt
     5761 tctcagcacg gatatcttca ctatttaaac taataggtgc ttccatagct aataatgcaa
     5821 ccatttgtaa catgtggttt tgcaccatat cttttagcgc accacttgat tcataataac
     5881 caccacgatc ttcaacacct agtatttcag aagatgtaac ttggatgttt gaaatatatt
     5941 tgttattcca taatggttca aacatcgcat tcgcaaaacg taatacctcg atattttgaa
     6001 ccatgtcttt tcctaaatag tggtcaatac gataaatttc ttcttcttta aatgatttac
     6061 gaatttgatt gtttaatgct tcggctgatt ttaaatcact accgaatggt ttttcgataa
     6121 caaggcgttt aaatcctttt gtatcagtaa gaccagaaga ttttagataa tcagaaataa
     6181 cgccaaagaa ttgtggtgcc attgctaaat agaatagtcg attacctttt aattcaaatt
     6241 ggctgtctaa ttcattacta aaatctaata attcttgata gctttcttca ttactaacat
     6301 catgtctatg atagaagaca tgttccataa acgcgtcaat tttgtttgta tcttttacgt
     6361 gcttttgaat tgatgatttt acttgattac ggaaatcatc attagtaatg tcacgacgtc
     6421 caataccgat gatggcaata tgttcatcta aattgtcttg ttggtagaga tggaatattg
     6481 atggaaacaa cttacgatgg cttaagtcac cagttgcacc aaagattgtg attaaacaag
     6541 ggatgtgttt gtttttagta ctcaagatta aaacctcaat tcttaattag atatatgatt
     6601 tattattata agataatcca ttcagcaggt cattcaatat gctcgacagt atgcagtgtc
     6661 ttaaatgaaa tatcgattca tgtatcatgt ttaatgtgat acttattaat gataagtata
     6721 acgtaattat caaaatttat atagttatgt ctacgttaaa gttagaaaaa ttaactagca
     6781 aagacgaatt tttaacagat tttgattcaa gtataaatta aaactaaatt gatacaaatt
     6841 ttatgataaa atgaattgaa gaaaaggagg ggcatatatg gaagttacat tttttggaac
     6901 gagtgcaggt ttgcctacaa aagagagaaa tacacaagca atcgccttaa atttagaacc
     6961 atattccaat tccatatggc ttttcgacgt tggtgaaggt acacagcacc aaattttaca
     7021 tcatgcaatt aaattaggaa aagtgacaca tatatttatt actcatatgc atggcgatca
     7081 tatttttggt ttgccaggat tactttctag tcgttctttt cagggcggtg aacaaaagcc
     7141 gcttacatta gttggaccaa aaggaattaa agcatatgtg gaaatgtcta tgaatttatc
     7201 agagtcacat ttaaactatc caattacata tattgaaatc gatgatcatc taacatatca
     7261 tcatgatggt tttactgtag aggcgcattt acttaatcat ggtattccat catatggata
     7321 tagagtaatg gcacctgaaa ctacaggtac aatcaatgtt gaagcgttaa aaaacattgg
     7381 tcttgaacca ggtcctaaat atcaagaagt aaaatctcat gatacatttg aacataatgg
     7441 tcaagtttat caatcaaaag attttagagg tgagtccaaa cagggtccaa ttgtagcaat
     7501 ttttggtgat acaaaaccat gttctaatga acgtgtgatt tctagagatg ctgatgtgat
     7561 ggtacatgaa gcgacctata tagatggtga aaagcattta gccaacaatt atcatcatag
     7621 ccatattgaa gacgtatttg cattgataaa agaagccaat gttaaacgta cgttgattac
     7681 acatttgagc aatcgctata atacagaaga tataaatgaa atttatcaaa cattgataca
     7741 aaatgaagat acgccgaatt ttaattttgt gaaagatttt gatagtttta aaatctaatg
     7801 acaaagtaat aaacaatgct tgccaaatag taaaaattta taaaaataac ccgtcaaaca
     7861 cagtgcttgc gatgatgcat acatgtgttg gcgggtttgt ttttattggt cttctatatt
     7921 agaaagttca atactacggt cgacggcagc atttagacaa tcttcgaaaa tagatactaa
     7981 atcatattgt gacaatgtat caaggccagc ttgtgtcgta ccacctttag aggtaatatt
     8041 ttttcttaat tgagccatgc tcaaatctga acgttcaatc atcttacttg tacctataat
     8101 aaggttgcgt atagattctt caacttgttc tttttctagt ccaagtttcg taccagcttt
     8161 aacgtattgc tcgaatacat gatataaaaa tgctgggccg cttccggtga tagctgttac
     8221 ttgatgtaaa tgatcctctg atacttcaat tactgaacca aatgctttaa ctaaatcgtt
     8281 aatttcatct ttagatttag ggtcaaagtt gtttgaaaaa ctaatgccag taactgagtg
     8341 tccaacttgc gcatttgtgt ttggcataat tctagcaact ggattttggc attctaattg
     8401 ctgtttaata taatcaatcg gaatacctgc cataattgaa atgaagcaat tgtcttttgt
     8461 aatatgtggt ttgatgcgtg ttgctagagc atcaaagtca tgtggtttgg tacctaaaaa
     8521 tacataatct gcatctttta ataatgtcgc atcatcataa ctatagttaa cacctagttt
     8581 ttcagcgaat gcttttaaag cttgttcatt agatttattt gttaaatata tatcattggc
     8641 atctaagttg cttgagttaa taattcctgt aaatatagct tgtgccatat taccagctcc
     8701 ataaaatacg agtttcatta acgtcacatc cctttagttt ttgatttgta tgttatattt
     8761 tcttattatt gtatatatat taggaaagat aatcaacttg tctcaattat gtaaagttga
     8821 taaattgcat tctatataat ggaaaaggaa ggttaattga tgaaaggaca acattttata
     8881 gtgactggtg gtacgagtgg tttaggttta tcgattgtcc gaaagttact agaaaataaa
     8941 gtgcatgtaa cgttgttggt gcgtgatgtt gataaagcaa ctagaatctt tgaccaggaa
     9001 ttggggaaaa ctataaatgt tattccttgt gacttaaatg atttgaagtc gatacaggca
     9061 ctgcaatttg aagacaatac attattcgat ggattcattt atagtgcagg tttaggttat
     9121 ttcaaatcta tttcagacca tagtttttca gaaatgatcg aaacgtatca gcttaattta
     9181 attagtttta atgttttata tactgtgttg agaccatatt taacatctaa cgcacatatt
     9241 gtaggtattt caagtcaagc tgcattttct acacaagcaa atgctgcaca ttatggggca
     9301 tcgaaagcag ggttttatgc attaatgaat gctttaagac tagagtcacc aaacttacat
     9361 atcatgacag ttaatgttgg cccgattgat acaccttttc atcaaaaagc cgacccatca
     9421 atgaagtatg ctaaaaagat ggggaaaatt atgcttgatg ctaatcagct tgcagaagat
     9481 atcatttatg gcattaaaac aaaacaattg gaaattaatc ggcctaaatg gatgcatcat
     9541 gcgttgaaaa tgtatcaaat tgcgcctaga ttttttgaac gatgtttccc taaattattt
     9601 aaaaataaag cataaatagg tgggatggca atgaactgtc aatcggagag tgtggtaaga
     9661 ctttgtgtac gatatgcgga acaactatca gtatttgaag aatttacagt gctggatatt
     9721 ttaagcgata tttctgtaga ccaaatttca gacagtacat tatattatac ttgtgaaaaa
     9781 ttcaaactac ttgtgttgca aggaaatgtg ttgggtgtac aagtaataac taataatgat
     9841 gaattaacat gcgaagtgaa atatagaaag atgttttaat aaaattatta aaagatgaag
     9901 tggcagaagt ttgcttaagg cactgccgca gggcggaatt actaaatact gatgcgtaag
     9961 gacaactctt taattactat tataagtgag caatcttaat cataaattat agcgaatttt
    10021 acataaacaa cagcagtaag gcatttttat aatgaaacgc ctcatactgc tgtttttatt
    10081 actgaaaatg acttctacac taaatgattg gtatattcca aatcctttac acgagctctt
    10141 gctgttttaa tctgatctaa actaacttta gtatgatagt tttcaatatt ttcttttaat
    10201 tggtcgacgc tacttgcacc tacaatgatg gaaccaagca cgtcatgtga tgttaaataa
    10261 ctaaatgtca atgcagataa attactttca atttctttaa tagaggctat tgtttcaccc
    10321 aattcatcat gagaataatc aaaaatacca tctttaaatt tattgtcgag cacattaaca
    10381 ctgtttgaag ttaataatcc tttagacaca ggtcctcttg ccaatacttt aacatcatcg
    10441 ttgtgaattg catctaataa tgattctgga cgattatcaa tcaaattgaa ttgagacatt
    10501 atcgtttcga tttgactatg ttttaaataa taatcaatta catttgggcg aatagaagaa
    10561 ataccgtaag cacgtataat tccttcttgt ttcaattcat caaatgcgct tattgtttcg
    10621 tctaatggat catcaatggt tccgccatga agttgatata aatcgatatg atcaatacct
    10681 aaacgcttta gtgaaccttt aaccgcctct ttaatatagg atttactcgg atcccatgtt
    10741 gtactgccat cttttgttaa acgattgcct acttttgtac caatgacaat atcatcgcgt
    10801 tgttgatatt taaaaagtgc cttaccaaca actgattcat ttataccttt atcgtacatg
    10861 tctgctgtat caaaataagt gataccattt tcaacagcac aatctataat ttgttcggct
    10921 ttttttaaat ctgtacctaa actcatgcaa cctagaccta attcagataa tgcaatacca
    10981 ctttttaata tatttttttg catgtctgtt ctcctttcga tacactttag ctaacaagca
    11041 tagtgtatca atatataata tgaatttaaa ggaacgtgat tataatggat ttaaatgaaa
    11101 aaacaattga tcgaacagtt atttataatg gcaaaattgt agatgtagaa attcatacag
    11161 tgacattacc aaacggtgaa acgtcaacaa gagaattagt ttatcataat ggtgcagttg
    11221 ctgtttgtgc attgacacct aaaaaagaag tcgtattagt gaaacagtat cgtaaaccag
    11281 tagaaaaacc attgttagaa attccggctg gtaaattaga agatgatgaa gatagagtcg
    11341 aagcggctaa gcgtgaatta gaagaagaaa caggatatat tgccaaagag ttaacacatg
    11401 ttgtagatat gtatggttca ccaggttttt gtgatgaaca attatcaata tattttacgg
    11461 ataatgtaga agaaggcaca gttcacttag atgaagatga atttgtcgaa gtcattaaag
    11521 ttccgattga gaatgttaaa tcgatgctaa tgaataaaga aatagaagat gcgaagacta
    11581 tcattgcatt gcaacaccta ttattaaatt ataatcattc taaataacaa atgagaaaag
    11641 cttgcatttt attgagaaaa ctgttagttt taattgtgaa gtttgaaata atttgtaatg
    11701 attttaatta ttagtagggg agtggacatc gttggaagaa cgattaaatc gcgttaagca
    11761 acaattacaa caatcatcat ataagctaac gccacaacgc gaagctactg ttagagttct
    11821 aattgaaaat gaaaaagatc atctaagtgc tgaagacgta tatctgaaag taaaagacaa
    11881 agcgcctgaa attggcttgg cgacagtata cagaacgtta gagttgttag ctgaactaaa
    11941 agttgtcgac aaaattaact ttggtgatgg cgtcgctcgt tttgatttaa gaaaagaagg
    12001 cgcaaaacat ttccaccatc atttagtatg tatggaatgt ggtcgtgtag atgaaatcga
    12061 tgaagatttg ttaccagaag ttgaaaatcg agttgaaaat gagttcaatt ttaaaatttt
    12121 agatcatcgt ttaactttcc atggtgtgtg tgaaacgtgc caagctaaag gtaaaggata
    12181 gtaaattgcg taggttaaat taaccttcgc tttttttaga ggtgtggtta tggaaacaat
    12241 tattgaagaa tatttgcgtt ttatacaaat tgaaaaagga ctaagttcta acacaattgg
    12301 tgcttataga cgtgatttga aaaagtatca agattatatg actgaacatc atatctcgca
    12361 tattgatttt atagatcgac aattaattca agagtgtttg gggcatttaa tagaccaagg
    12421 gcaatctgct aaatccattg cgcgatttat ttcaacaatc cgtagttttc atcaatttgc
    12481 tataagagaa aaatatgcgg cgaaagatcc aacggtatta ttagattcac caaaatatga
    12541 caaaaaattg cctgacgttt taaatgttga cgaagtgttg gctttattag aaacgccgga
    12601 tttaaataaa attaatggtt atcgtgatcg tacgatgtta gaacttctgt acgcaacggg
    12661 aatgcgtgta tctgaattga tacatttaga gctagagaac gtgaacttaa taatggggtt
    12721 tgtacgcgta tttggtaaag gcgataaaga aagaattgtg ccattaggcg acgcagtcat
    12781 tgagtactta actacttata ttgaaacgat tagaccgcaa cttttaaaaa agactgttac
    12841 tgaagtctta tttttaaata tgcatggtaa acctttatca cgacaagcaa tatggaaaat
    12901 gattaaacaa aatggtgtaa aggcaaacat taaaaagacg ctaacgccac atacgttgcg
    12961 ccattctttt gcgacacatt tattggaaaa tggcgcagat ttaagagcag tgcaagagat
    13021 gttaggtcac tctgacatat ctactaccca actctataca catgtttcaa aatctcaaat
    13081 tagaaaaatg tataaccaat ttcatcctag agcataaagt gaaccataac tcaaaagtca
    13141 caatacacat gactaaaaat gtctgtgcta ttgtggcttt tgagttatgg ttgattaatt
    13201 acgtctatgt tttcttaatt gaatcgcttc ttcttttgct gcaatcactt ctgaacgatc
    13261 acgccgcatg tgatggtcta caataaaagg atctgttgct gtttcgtgat tataatcata
    13321 atctggatag ttggccttga tgatgcgctc aaagtctgga gttattggta atataacaga
    13381 tgaaaaaggc tttgctgcat tcaattttgc aatctgtttc tcaattaaca actgataatc
    13441 atttaaatta aggtataacg catctctatc tttagcattt tgtattattt ctttagattt
    13501 attaaaagac ttataggcgc cttttaaatt attgcggcga taatggtaac aagcagttgc
    13561 aaacaagatt aaactaacaa ctgcatcttg cttactgtag ttattttcag ctttccatgc
    13621 atcttctaaa atgtcatgac ataggaaata atgttgctta gtatgaaatt gataatagaa
    13681 atttatcagt gcctgttgca ttttgttatc accccaattt aaaagtaagt tattttcatg
    13741 ctataatatt ttagagaatt atgcacatat gacgcaatac gaggtagata ttatgtatga
    13801 agttaaatta gatgctttca atggaccatt agatttatta ctgcatctta tccaaaaatt
    13861 tgaaatagat atttatgata ttcctatgca agcattaaca gagcagtata tgcagtacgt
    13921 tcatgcaatg aaacagcttg aaattaatat tgcaagtgaa tacctagtat tagcgtcaga
    13981 actcttaatg attaaaagta agatgctatt accacaatca acatcagata tagatgttga
    14041 tgatgaccca cgggaagatt tagttgggcg tttaatagaa tatcaaaatt ataaagaata
    14101 tactgctatt ttaaatgaca tgaaagaaga aagagatttt tattttacaa aaagaccgac
    14161 agagttatct catttggaaa cagatgaatc ttgggatcca aatcatacga ttgacttaac
    14221 tgaattaatt gtagcttatc aaagagttaa aaatagagtt gagttaaata cacctaaatc
    14281 tgttgaaatc cgaaaagaga catttaccat tcaacaagct acagaacaag tgacatcgag
    14341 attgaaagat aaagatcatt ttaacttctt tagtctgttt acgttttctg agccaattga
    14401 acaagtagtc actcactttt tagctatttt agagatgtca aaagcaggaa taattaatat
    14461 tgagcaacaa cgtaattttg aagatattaa cattgttaga ggagtgaact atcattttgg
    14521 ataatcatgg tatattagag tcgctattat ttacagctgg cgatgaaggt ttagatgaaa
    14581 aacagctatt agaaattttg gatatatcga aagaccaact cgtagaatta attgaaaatt
    14641 attcatcaca tggattaata atacaacgat ttggaacgac gtatgtttta acgactaaaa
    14701 aagaagcgtc aatgtatatt gaacaattaa ttgaacaaaa gtcacaaatg aaattatcac
    14761 aagcagcaat ggaagtacta tcaattattg cttataacca accattatca agaagtgata
    14821 ttgaattaat tcgtagtatc aattcagatg gtgcggtcaa gacattgatt gccaaaggac
    14881 tagttgaggc taaagtggtc aatgaacagc gtagccaaca attaattact actgatttat
    14941 ttttaaatgt atttggtatt tccaatatag aagatttgcc gacaactgaa gaagacgatg
    15001 aagaaatgga tgcttttttc agtaatctag tcaatcaaaa aggagaaaat aatgactaaa
    15061 gaattagaac gattacaaaa acgtatagct aatagcggtt atacttcaag acgtaaagca
    15121 gaaactttaa tttctgaagg aaaagtgaaa gtgaatggta cgactgtcac tgaattagga
    15181 acaaaagtga agccatcaga tacgattgaa gttgaaggta ttaaaataga gctagaagac
    15241 aaaatttaca ttttattcca taaaccaact caagtgataa caagtgtatc tgatgataga
    15301 ggacgtacag tggttacaga ttactttaaa gatatagaag cgcgtattta tcctgttggt
    15361 cgtttagact atgatacttc tggattgtta ttacttacaa atgatggtga atttactaat
    15421 ttaatgacac atccaagata tcaaattaag aagaaatatg tagcaaaatt aaaaggttac
    15481 ttaatgagag aagaagtgaa agcgctagaa aaaggtattg aattagaaga tggtatgacg
    15541 caaccggctg aagtgaaagt taagaaacaa gataaagata aaaacacaac attggttgaa
    15601 attacaataa cagaaggtcg taatcgtcaa gtcagaagaa tgttcgaaca ttttggtcat
    15661 caagtgacga aactgtctcg tattgaatat gggcctttaa atgttgtcgg tttgaatgct
    15721 ggtgaaggac gtgtattgac gcctcatgaa gtaaaagtga tgcgtcattt agcagaacat
    15781 gggaaataat taaataaaat atgtatttat cacaaagttt gagaattttt tcacaaaatt
    15841 ttaggttttt aataactgta tgcgctttcc tgtgatataa tacttttgta aagaaaagca
    15901 tgtgtgggag gtatgacctg tatgtcgaac gaaatactta tcgtagatga tgaggataga
    15961 atcagaaggt tacttaaaat gtatttagaa agagaatctt ttgaaatcca tgaggcaagt
    16021 aatggccaag aggcttatga acttgcaatg gagaataatt atgcttgcat actactagat
    16081 ttaatgttgc ctgaaatgga tggtatccag gtggcaacta aattgcgtga acataaacaa
    16141 acaccgatta ttatgttgac tgctaaaggt gaagaaacaa accgtgttga aggttttgag
    16201 tctggtgcag atgattatat cgtcaaacca ttttcaccaa gagaagtagt cttaagagtt
    16261 aaagcacttc taagaagaac gcaatctaca actgtagaac aaagcgaacc tcacgcacgt
    16321 gatgtgattg aatttaaaca tttagaaata gataatgatg cacatcgcgt acttgctgat
    16381 aatcaagaag ttaatttgac tcctaaagag tacgaattat taatatattt agctaaaaca
    16441 ccaaataaag tatttgaccg tgaacaatta ttaaaagaag tttggcatta tgaattctat
    16501 ggtgatttaa gaacagttga tactcatgtt aaacgactta gagaaaagtt aaatcgtgtg
    16561 tctagcgaag ctgcgcatat gattcaaaca gtctggggcg ttgggtataa atttgaggtt
    16621 aaatctaatg atgagccggc taaatagtgt cgtaattaaa ctgtggttaa ctattatttt
    16681 aatagtgacg acagttttaa ttttattaag tattgcttta attaccttta tgcaatacta
    16741 tttcacacaa gaaactgaaa atgccataag agaagatgct agacgtataa gttcactggt
    16801 cgaacaatca cataataaag aagaagcaat aaaatatagt caaacattaa ttgaaaatcc
    16861 tggtgggttg atgattataa ataataaaca tcgtcaatca acggcttcgc tttctaatat
    16921 taaaaagcaa atgttgaatg aagtagtcaa caacgaccat tttgacgatg tgtttgataa
    16981 aggtaaatct gttactcgaa atgtaacgat taaagaaaag ggctcatctc aaacatatat
    17041 tttgttaggc tatccaacaa aagcacagaa gaatagtcat agcaaatata gtggagtctt
    17101 tatatataaa gacttgaaat caatcgaaga tacaaataat gctattacga ttatcactat
    17161 aattacggct gttattttct taacaattac aacagtcttt gcgtttttct tatcgtcaag
    17221 aattacaaaa cctttaagac gtttaagaga ccaagctaca cgtgtatctg aaggggatta
    17281 ctcttataaa ccttctgtca caacgaaaga tgaaattggt caattatcac aggcatttaa
    17341 tcagatgagt acagaaatcg aagagcatgt cgacgcatta tccacatcta aaaacattag
    17401 agacagctta attaactcta tggtagaagg tgtcctaggt attaatgaga gtcgacaaat
    17461 tatcttatcg aataagatgg cgaatgatat tatggacaat attgatgaag atgctaaagc
    17521 tttcttatta agacaaattg aagatacttt taaatcaaaa caaacggaaa tgcgtgattt
    17581 agaaatgaat acacgattct ttgttgtgac cacaagctat atcgacaaga ttgaacaagg
    17641 aggtaaaagt ggtgttgttg tgacagttcg tgatatgact aatgagcaca atctagatca
    17701 aatgaagaaa gatttcattg ctaatgtatc acatgaatta cgtacaccga tatcattact
    17761 tcaaggttat actgaatcaa ttgtagatgg tattgttaca gaaccggatg aaataaaaga
    17821 atcgcttgcc attgtccttg atgaatcgaa acgtttaaat cgtttagtta atgaattgtt
    17881 aaatgtcgct cgcatggatg ctgaagggtt atccgtaaat aaagaagttc agcctattgc
    17941 agcgttacta gataagatga aaattaagta tcgtcaacag gctgatgatt taggtctaaa
    18001 tatgactttt aattattgta agaagcgtgt ttggacttat gatatggatc gcatggacca
    18061 agtactaacg aacttaattg ataatgcatc acgttatacg aaacctggtg atgaaattgc
    18121 aattacttgt gatgaaaatg aaagcgaaga tattttatac attaaagata caggtacagg
    18181 cattgcacca gaacatttac aacaagtatt tgatcgtttt tataaagttg atgcagcgag
    18241 aacgcgaggt aaacaaggta ccggtttagg tttgttcatt tgtaaaatga ttatcgaaga
    18301 gcatggtggt tccatagatg ttaaaagcga attagggaaa ggcacaacat ttattattaa
    18361 actaccaaaa ccagaataaa attgaatata gttatttcag aacgcatgtt attgattaga
    18421 gactctaatt tatagcatgc gtttttttat tgatgtgggg agttttgtat gtggattagg
    18481 atttagggtt tagggttttt gcgaatatca actattaaat atattactaa tttatataaa
    18541 aatataaagt ttgataaagt tatttatttg attataaaaa tagggtaaaa tatagatgta
    18601 ttgtaataat taaattattc gaggtgtcat atgaaaaaat tcattggatc agttttagct
    18661 acgacattaa ttttaggggg atgttccacg atggaaaatg aatcaagtaa agacacgaat
    18721 acagaaacaa aatcagtacc agaagaaatg gaagcttcaa aatatgtagg acaaggcttc
    18781 caaccgcctg cagaaaaaga tgcgattgaa tttgcgaaga agcataaaga taaaattgcc
    18841 aaacgaggcg aacaattttt tatggataac ttcggtttaa aagttaaagc taccaatgtt
    18901 gtaggtagtg gcgacggtgt agaagtattc gtgcattgtg atgaccacga catcgtattt
    18961 aatgcgagta ttccatttga caaatcgata atagagagtg atagctcatt aagaagtgag
    19021 gataaaggcg atgatatgag tactttagta ggtacagtgt tgagcggctt tgaatataga
    19081 gctcataagc aagagttgga tagtttaaca gaagttttga aagaatataa aagtaaatat
    19141 aaatatactg gttatacaga aaatgcaatt atgaaaacgc agaatagtgg ttttagaaat
    19201 gaatattatt atttgactgc aattccatat actttagatg aatataaaaa gtattttcaa
    19261 cccttaatca aagaggatga taagactttt agtgatggta tgaaaaactc aaaaaaacaa
    19321 cttaaagata aatctcgtcc atatgttgtt acaactttat ttagcactaa agacaattac
    19381 actaaagata atacaataga cgagatgatt gatttttctg aagtactaaa aaagaaaaag
    19441 aatattcctc atgatttgaa tgtttctctt caaattactg ataaatatat aaattcagca
    19501 agacctagtt acagtaagaa agattcaata gaagtaggag tatttaatca tgaaaaaaat
    19561 taatgataga gatttagcag aattaagtgg atactgggca tatcaagatt tggacaaaag
    19621 atatgtaatt agagtaaata ataataaata tagactaatt gaagattatt cagacaatcc
    19681 taattctaaa agttttggtg gtacagatgc aaaagttttt gaattggtaa atagcaataa
    19741 tgaattaaca ggacaacaag ctattatctt ccaaggtaca aataataata agagtatcgg
    19801 aagcaacaat ccttttcgtg ggaaggttgc tgatgattgg attgaaaata taaagttaat
    19861 gaatgataaa aataagtcaa cttctttatt agaacaaaat aatgcttata ttaaatcgta
    19921 tgaacagaaa ctaaaagatg cagatacact taaaagctct gattttttaa ggaaatataa
    19981 gaaaaatccg tctacgtata aaaacaaaac tattaagtct gatggaggca attctcaagg
    20041 gggagcaagt gctaaccacc aagggatgat taacccgaat aaaaatatag tttctacaaa
    20101 ccctgccatg ttaccaaagt caatatggga aaatttcaaa tctcaaaact ttgaaaatat
    20161 gattaattat catagtaagt ttgatatttt atcttggctg caggatccat ttgccactcc
    20221 gacactagga aaacgagtga atttagaaac tggtgtgcca acgatggatg gattagtaaa
    20281 tagtcattta ggttataaaa gaaaattaaa tccaagatat aatacttata aggatttagc
    20341 agtttataaa attaaatcag ttaaagatac tatgattaaa aatggtaaaa aagtcaaaaa
    20401 aactattgaa atcgatataa atatggatga tagaattcca attaatgtgt ggacaggaga
    20461 ttcaatagcg agaacgggta aagggactcc tataaaatta aatctagaaa atcttagtgc
    20521 attaagtaat ttggtaactg gtgaaacaag taatatgtta accgattgtg taaattattt
    20581 gaatgagagc tataatattt cacaggttga aaatgctaat tttggagaaa gaaaacataa
    20641 acttaaacaa gattttaaaa agattgtcga agttgatatc cttgaaggaa tgagtagaga
    20701 attaacttct ttgaaaaagg atgcatttag cgcaattgat aatgtcaaaa aaaatcttgt
    20761 tggaattgct ttcatatttt catcagttgc agtccttatt tttgaattaa atactattga
    20821 agaaatgctt gaagatttag ataaaaaatt acaacgtggc atagaaagtt tacatgattc
    20881 attggataac gtaattaaag aaatgtttaa aaatttagat catgattttg aagatggcgt
    20941 gacagaggaa atgatgaagc atttaaatgt tgtgaataat aatataaatt ttgtaaaaaa
    21001 acaaaatgat gtatatggag agcagataac cgatattaaa aatataatgt ctcatcaaga
    21061 tgcaacggta atggatggaa acttatctat taattatagc ggtgaacata tgatttcggg
    21121 tcaagtacaa tcttctaatt atttaactag aaaaatgaca attttaaaaa accatattga
    21181 taatgcagtt aacaaaatta gtgattatgt tcaagaagct tacaataatt attttgaacc
    21241 agtattaaac tacattcttg aagttataca ttcggtagaa acagctgtga gacatattta
    21301 tgaaataagc agtttaatat acaaagtaac aatgaaaata aagataaagg ctatgggaat
    21361 aaatgtcgac gaaattgata aaaaccttga agttttaaaa agtaaactta aaaatttaga
    21421 tgaattttta aataacttaa aaacagcttc accaatttta gaaaatcact tagatgacat
    21481 tgttagaaat atgaagccat taattgttaa tcaaattttt gaaccatctc attatgatga
    21541 tatgtttatt ttaaacactc aagctcatgc acgtcttgat caaatggcac aacagtttga
    21601 agttgtttgt aatggcttga acgaaaatga agggcaagct attcaaacaa tggatcaatc
    21661 tgcttcgtta attcgttcaa acttaattca agttaaagaa caattagaaa aactagcagt
    21721 atactaatta tttattaaaa gctacttgtt ttcattgaga ataagtagct ttttcaaaca
    21781 taaaagtttt acaaacacat aaataggtga tgaaatatgc ttaaaaaggc aaaattaatc
    21841 ttaatagcaa cgctactgct atcaggatgt tcaactacca ataacgaatc taataaagaa
    21901 acaaaatctg taccagaaga aatggaagct tcaaaatatg taggacaagg cttccaacca
    21961 cctgcagaaa aagatgcgat tgaatttgcg aaaaagcata aagacaaaat tgccaaacga
    22021 ggcgaacaat tttttatgga taacttcagt ctaaaagtta aagctacgaa tgttgtaggt
    22081 agtggcgacg gcgtagaagt atacgtgcat tgtgatgacc acgatatcgt atttaatgcg
    22141 agtattccat ttgacaaatc aattattgac agtgatagct cattaagaag tgaggataaa
    22201 ggcgatgata tgagtacttt agttggtaca gtgttgagtg gctttgaata tcgagcacaa
    22261 aaagaaaagt atgacaattt atataaattt ttcaaagat
//
LOCUS       DATTQF010000028        21235 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.028, whole genome shotgun sequence.
ACCESSION   DATTQF010000028 DATTQF010000000
VERSION     DATTQF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.028"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            159..956
                     /locus_tag="VB241_000973"
     CDS             159..956
                     /locus_tag="VB241_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1244277.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKDYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            1205..1696
                     /gene="lspA"
                     /locus_tag="VB241_000974"
     CDS             1205..1696
                     /gene="lspA"
                     /locus_tag="VB241_000974"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP1244278.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRVLTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            1696..2613
                     /locus_tag="VB241_000975"
     CDS             1696..2613
                     /locus_tag="VB241_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1244279.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKDLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELAQDFEDLLDTIRKRDA"
     gene            3015..3542
                     /gene="pyrR"
                     /locus_tag="VB241_000976"
     CDS             3015..3542
                     /gene="pyrR"
                     /locus_tag="VB241_000976"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP1244280.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            3760..5067
                     /locus_tag="VB241_000977"
     CDS             3760..5067
                     /locus_tag="VB241_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP1244281.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIFVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            5095..5976
                     /locus_tag="VB241_000978"
     CDS             5095..5976
                     /locus_tag="VB241_000978"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP1244282.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5994..7268
                     /locus_tag="VB241_000979"
     CDS             5994..7268
                     /locus_tag="VB241_000979"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP1244283.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGIDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAADCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            7270..8370
                     /gene="carA"
                     /locus_tag="VB241_000980"
     CDS             7270..8370
                     /gene="carA"
                     /locus_tag="VB241_000980"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP1244284.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAILMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            8363..11536
                     /gene="carB"
                     /locus_tag="VB241_000981"
     CDS             8363..11536
                     /gene="carB"
                     /locus_tag="VB241_000981"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP1244285.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHNFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQCLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            11646..12338
                     /gene="pyrF"
                     /locus_tag="VB241_000982"
     CDS             11646..12338
                     /gene="pyrF"
                     /locus_tag="VB241_000982"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP1244286.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            12338..12949
                     /locus_tag="VB241_000983"
     CDS             12338..12949
                     /locus_tag="VB241_000983"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP1244287.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12979..13191
                     /locus_tag="VB241_000984"
     CDS             12979..13191
                     /locus_tag="VB241_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244288.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTINHNDDLFKHVKDILRKQGQI"
     gene            13628..14029
                     /locus_tag="VB241_000985"
     CDS             13628..14029
                     /locus_tag="VB241_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1244289.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(14292..15989)
                     /locus_tag="VB241_000986"
     CDS             complement(14292..15989)
                     /locus_tag="VB241_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEP1244290.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYEHIQKMKQS"
     gene            16264..16887
                     /gene="gmk"
                     /locus_tag="VB241_000987"
     CDS             16264..16887
                     /gene="gmk"
                     /locus_tag="VB241_000987"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP1244291.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            16887..17105
                     /locus_tag="VB241_000988"
     CDS             16887..17105
                     /locus_tag="VB241_000988"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP1244292.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            17321..18520
                     /gene="coaBC"
                     /locus_tag="VB241_000989"
     CDS             17321..18520
                     /gene="coaBC"
                     /locus_tag="VB241_000989"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEP1244293.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            18520..20928
                     /gene="priA"
                     /locus_tag="VB241_000990"
     CDS             18520..20928
                     /gene="priA"
                     /locus_tag="VB241_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP1244294.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIEVPSTLLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEERTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7509 a         3058 c         4011 g         6657 t
ORIGIN      
        1 tatgtaaaaa atgtgtgatt tactaaaaaa aaattattca ggaatggtac aaattctctg
       61 aagcttataa atgcgttata gttgctattc tcaattatgt tcgcgataat tttaagtaaa
      121 agtaagcaca gatattgaat ttgataggag ttaattgaat gtatcataac agtaacgcaa
      181 actttgtaaa tggtatcact ttaaatgtga gagataagaa tgaattaaag ccattttatg
      241 aggacgtatt aggattaaat attataaatg agacattaac atcgatccaa tatgaagtag
      301 gtcaaaataa tcatgtcatt acacttgttg aattacaaaa tgggcgtgaa cctttaatgt
      361 cagaagcggg actgtttcat atcgcaatta aactacctca aattagtgat ttagctaatt
      421 tactaattca tttaagcgaa tatgatattc cagttaacgg aggtatacag cctgcttcgt
      481 tatcattatt ttttgaagac ccggaaggaa acggttttaa attttatgtt gataaagacg
      541 aagcgcaatg gacgaggcaa aatgatttag taaaaattga tattagacca ttaaatgtac
      601 cgagattagt gagtcatgca acaaaattgt tatggttagg tattccagat gacgctatta
      661 taggtgcatt gcatattaag acaattcatt tatcagaggt aaaagactac tacctcgatt
      721 attttggatt agagcaatcg gcatatatgg atgattattc aatattttta gcatcgaatg
      781 gctattatca acatttggcc atgaatgatt gggtatcagc aacgaaacgt gtagaaaatt
      841 ttgatacgta tggattagca attgttgact ttcattatcc tgaaacaaca catttaaatt
      901 tacaaggtcc ggatggtatc tattatcgct ttaatcatat cgaagttgaa gattagtaaa
      961 tactttgaat ggacgaacca tataatgaat cgtttttaat gatcttttta tacaagctat
     1021 gaaggcagtc ttatgaggtt tcccatgagg ctgctttcat aatttttaat gtcaaaacat
     1081 agtagtttat caagtattga gttagtaaca ttagatttaa tgtaatatgc ttactttttt
     1141 tattagcagg tgtaagctat aatataaaga gttgtcttat ggacgattga ttggaggaac
     1201 gaaaatgcac aaaaaatatt ttattggcac ttccatttta atagcagtat ttgtcgttat
     1261 atttgaccaa gttactaaat atattatagc tactacaatg aaaattggag attcatttga
     1321 agtgataccg cactttttaa acataacatc acatcgaaat aatggtgctg catggggaat
     1381 attgagtgga aaaatgacat ttttctttat tattaccatt attatattaa tagccttagt
     1441 atatttcttt attaaagatg ctcaatataa tttgtttatg caagttgcta ttagtttact
     1501 ttttgcaggt gcacttggaa actttattga tagagtttta acaggagaag ttgttgactt
     1561 tattgataca aatatttttg gttatgattt tccaatattt aatatcgcag attcaagttt
     1621 aacaattggt gtaatattaa ttattattgc cttattaaag gatacttcca ataaaaagga
     1681 gaaggaggtt aagtaatgga gacttatgaa tttaacatta cagataaaga acaaacaggt
     1741 atgcgtgtag ataagttgct gcctgaatta aatagtgatt ggtctcgtaa ccagatacaa
     1801 gattggatta aagcaggttt agtcgttgca aacgataaag ttgttaaatc taattataaa
     1861 gtgaaactta atgatcatat agttgtcact gaaaaagaag tggttgaagc tgacattcta
     1921 cctgaaaatt taaatttaga tatttattat gaagatgatg acgttgcagt tgtttataaa
     1981 ccgaaaggca tggtagttca tccatcacca ggtcattata ccaatacatt agttaatggt
     2041 ttaatgtatc aaattaaaga tttatcgggt attaatggag aaattcgtcc aggtattgtt
     2101 caccgtatag atatggatac ttctggttta ttaatggttg ctaaaaatga tattgctcat
     2161 cgtgggcttg tagaacaatt aatggataaa tctgttaaaa gaaaatatat cgctttagtt
     2221 cacgggaata ttcctcatga ttacggaaca atcgatgcgc caattggtag aaacaaaaat
     2281 gatcgtcaat ctatggctgt tgttgatgat ggtaaggaag cagtgacaca ttttaacgta
     2341 ctagaacatt ttaaagatta tacgcttgtt gaatgtcaac ttgaaacagg acgtacgcat
     2401 caaatccgtg tgcacatgaa atatattggc ttcccattag ttggtgatcc aaagtatgga
     2461 ccgaaaaaga cattggatat tggtggtcaa gctttacatg ctggacttat tggattcgaa
     2521 catccagtaa caggtgaata tattgaaaga catgctgaat tagcacaaga ctttgaagat
     2581 ttattagata caattcgaaa aagagatgca taattgtgtc gtgctataat tacgataaca
     2641 ttattatgta aactataagt atttatcgtt ttcgtttttt taaaaatgaa aacacattat
     2701 aaaatagatt aaggttaaca tttttactga gaatggttaa tttttcaata tagattgaca
     2761 aagcgcctga ttagtattat ccttataaca gtttaagtac aacataaaga gtctttaatt
     2821 gagcgttcag agagacgtca aagacatgaa aatatagata tatactaaaa tgaatcataa
     2881 cactttcaca tatgcaagaa taagtagtaa tactacgatt cttctatgta aataggatga
     2941 ttgtatatac taaacctcat gtcgatatta aagacatgag gttttttgtt tctaaatttt
     3001 gaaagggtga gaatatgtct gaacgtatca taatggatga tgccgcaata caacgtacag
     3061 taacgagaat cgctcatgaa attttggagt ataacaaagg tactgataat ttaattcttt
     3121 taggtatcaa aacaagaggt gaatatttag cgaatcgtat acaagataaa attcatcaaa
     3181 ttgagcaaca acgtatacct actggaacaa ttgatattac atactttaga gatgatatag
     3241 agcacatgtc atcacttacg acaaaagacg caatagacat cgacacagat attacagata
     3301 aagtagtcat cattattgac gatgtgctgt atactggtcg aacggttcgt gcttcacttg
     3361 atgctatttt gctaaatgct agacctatta aaattggttt ggctgctttg gttgatcgag
     3421 gacatcgtga gttaccaatt cgagcagatt ttgttggtaa aaatatacct acttctaaag
     3481 aggaaacggt aagtgtctat ttagaagaaa tggatcaaag aaatgcagtt ataattaaat
     3541 aatacccctt ttaattcagt acgagagact gaaaaaggtg ggggagttga atgataaata
     3601 agttttcata tgcttgttta tctaataata ccctcatgtt cgacaactat aagtcaattt
     3661 ctatggacga aaatgacttg tagcaacttg tcacactttt tgtctcttta catatactga
     3721 tgtaaagaga tttttttatt atcggaggaa atgaatatta tgcaaaatga tgaaatgttt
     3781 gaacgaacag taaaacctgt actagatgta aatgaaaaac cacaaccagc gcaatgggca
     3841 tttttaagct tacaacattt atttgcgatg tttggcgcaa cagtactagt accattctta
     3901 acaggattac caatatcagc agcgttacta gcttcgggaa tcggtacatt actttatatc
     3961 ttaataacga aggcgcaaat accagcatac ttgggatcta gctttgcatt tatcacacca
     4021 attatcacgg gattaagtac gcatagctta ggagacatgc ttgtagcatt attcatgagt
     4081 ggtgttatgt acgtcatcat cgggattcta atcaaattaa gtgggacagc atggttaatg
     4141 aaattattac caccagttgt tgtaggacca gtcatcatgg taattggttt aagcttagcg
     4201 cctactgcag tcaatatggc aatgtatgaa aatcctggag atatgaaagg ttacaatatc
     4261 agtttcttaa ttgttgcaat gataaccttg cttgtaacaa tattcgttca aggatttttt
     4321 aaaggattcc tatctttaat tccagtactt gtaggtatta tcgtaggtta tgtggtagcg
     4381 atttttatgg ggattgtgaa atttgatgca attatgtcag caaaatggat agatttccct
     4441 catatttatc tgccatttaa agattatgta ccttcatttc acttaggact tgtacttgta
     4501 atgattccga ttgtgttcgt aacagtaagt gaacatattg ggcaccaaat ggtactgaat
     4561 aaaatcgtag gtagaaactt ctttgaaaag ccaggacttg ataaatcaat cattggtgat
     4621 ggtgtttcta caatgtttgc cagtattatt ggtggaccac caagtacaac atatggtgaa
     4681 aatatcggtg tattagcgat tacaagaata tacagtattt acgtcattgg tggtgcggca
     4741 gttatagcaa ttgttttagc attcattggt aagttcactg cattaatttc ttctatacct
     4801 acaccagtta tgggaggagt atctatatta cttttcggta ttattgcagc aagtggctta
     4861 agaatgttag ttgaaagcaa agtagatttt gcgaacaatc gaaatttagt tatagcttca
     4921 gtaattttag ttgtaggtat cggtaattta gtatttaact taaaagaaat tggtatcaac
     4981 cttcaaattg aaggaatggc attagctgca ctttcaggaa ttattttgaa cttaatctta
     5041 cctaaagaga aaaaacaaaa caattaagat ttacaaatta aggagggcgc ttttatgaat
     5101 catttattat caatggaaca tttatctaca gatcaaatat acaaacttat ccaaaaggca
     5161 agtcaattta aatctggtga acgtcaacta ccaaactttg aagggaaata tgtcgcaaat
     5221 ttattctttg aaaattctac tcgaacaaaa tgtagttttg aaatggcaga acttaagcta
     5281 gggttaaaaa cgattagctt tgaaacatca acatcatctg tttcaaaagg tgaatcttta
     5341 tatgacacat gtaaaacttt agaaagtatt ggctgtgatt tattagtcat tagacatccg
     5401 tttaataact actatgaaaa attagcgaat attaacatcc caattgcgaa tgctggtgat
     5461 ggtagtggac aacatccaac acaaagtttg cttgatttaa tgacgatata tgaagaatat
     5521 ggatattttg aaggcttgaa tgtattgatt tgtggcgaca ttaaaaattc acgtgtcgca
     5581 cgtagtaatt accatagttt aaaagcatta ggtgcaaacg taatgtttaa tagcccaaat
     5641 gcttggattg atgattcttt agaagcgcct tatgtaaata tagatgatgt tatagaaaca
     5701 gtagatatag ttatgttatt aagaattcaa catgaaagac atgggcttgc agaagaaact
     5761 agatttgcag cagatgatta tcatcaaaag catggcttaa atgaagtgcg ttataacaaa
     5821 ttacaagaac atgctattgt catgcatccg gcacctgtga atagaggagt agaaatacaa
     5881 agcgatttag tagaagcttc aaaatcaaga atttttaagc aaatggaaaa tggcgtttac
     5941 ttaaggatgg cagtcattga tgaattatta aaataggtaa ggggacgaaa atgatgaaat
     6001 taattaaaaa cggtaaagta ttacaaaatg gtgaattaca acaagcagat attttaattg
     6061 atggtaaggt aattaaacaa attgcacctg caattgaacc aagcaatggt attgacatca
     6121 tagatgcgaa aggtcacttt gtgtcacctg gatttgtcga tgttcatgtt catttacgcg
     6181 aacctggtgg tgaatataaa gagacaattg aaactggtac taaagctgct gctagaggcg
     6241 gatttacaac tgtatgtcca atgcctaaca caagaccggt accagattct gtagaacatt
     6301 ttgaagcttt acaaaaatta atcgatgaca atgctcaagt acgtgtatta ccttatgctt
     6361 caattacaac acgtcaatta ggtaaagaat tggttgattt cccagcacta gtaaaagaag
     6421 gtgcctttgc gtttacagat gacggtgtag gagtacaaac tgcaagcatg atgtatgaag
     6481 gcatgattga agctgcaaaa gtaaacaaag ccatcgtagc acactgtgaa gataattcat
     6541 taatctatgg tggtgcaatg catgaaggga aacgcagtaa agagttaggt ataccaggta
     6601 ttccaaacat ttgtgaatct gttcaaatcg caagagatgt actattagct gaagcagcag
     6661 attgtcatta tcatgtatgt catgtttcta ctaaagaaag tgttagagtc attcgtgatg
     6721 ctaaacgcgc aggcattcat gttacagctg aagttacacc acaccattta ttgttaacag
     6781 aagatgatat tcctggtaat aatgccattt ataaaatgaa tccaccattg agaagtactg
     6841 aagatagaga ggctttgtta gaagggttac tagacggtac aattgactgt atcgcaacag
     6901 accatgcacc acatgcacgt gatgaaaaag cacaaccaat ggaaaaagca ccattcggaa
     6961 ttgttggcag tgaaacagca ttcccattat tatatacgca ttttgtaaaa aatggtgatt
     7021 ggacattaca acaattagta gattacttaa caattaaacc atgtgaaaca tttaatttag
     7081 aatacggcac attaaaagaa aatggttatg cagatttaac aatcattgat ttagatagtg
     7141 aacaagaaat taaaggagaa gatttcttat caaaagcaga taatacacca tttatcggct
     7201 ataaagttta tggaaatccg atcttaacaa tggttgaagg cgaagttaaa tttgaggggg
     7261 ataaataata tgcaaagcaa acgttatcta gtgttagaag acggttcttt ttacgaaggc
     7321 taccgtttag gatctgataa cttaactgta ggagaaattg tatttaatac agcgatgaca
     7381 ggttatcaag aaactatttc agatccatca tatacaggtc agatcattac ttttacgtat
     7441 ccattaatcg gtaattatgg tatcaataga gacgattttg aatcattagt acctacatta
     7501 aacggtattg tagtgaaaga agcgagtgcg catccaagta attttagaca gcaaaagaca
     7561 cttcatgacg ttttagaatt gcatcaaatt ccagggattg caggtgttga tacaagaagt
     7621 attacgcgta aaattcgaca acacggtgtg ttaaaagctg gttttactga tcgaaaagaa
     7681 gatattgatc aacttgtcaa acatttacaa caagtagaat tacctaaaaa cgaagtagaa
     7741 atcgtttcga ctaaaacacc gtatgtttcg acaggtaagg atctaagtgt cgtacttgta
     7801 gactttggta agaagcaaaa tattgttcga gaattaaacg tcagaggttg taacgtcaca
     7861 gttgtaccat atacaactac tgccgaagaa attttagcaa tggctccaga tggcgttatg
     7921 ctatcaaacg gaccaggtaa tcctgaagtt gtagaatgtg cgattctaat gattcaagga
     7981 attttaggga aaattccgtt ctttggtatc tgtctaggac atcaactttt tgcattatct
     8041 caaggagcaa gctcatttaa aatgaagttt ggtcatcgtg gtgcgaacca tccagttaaa
     8101 aatttagaga ctggaaaagt tgatattacg agtcaaaacc atggatatgc aatagatata
     8161 gattcgttaa aaagtactga tttagaagtt actcatcttg cattaaatga tggtactgta
     8221 gaaggtttaa aacataaaac attaccagca ttttctgttc aataccatcc tgaagcaaat
     8281 ccaggaccgt cagattcaaa ctatctattt gatgattttg tagcaatgat gactaatttt
     8341 aaggaaaagg agcgtcatat caatgcctaa acgtaatgat atcaaaacaa ttttagtaat
     8401 agggtctggg ccaattatca taggtcaagc agctgaattt gattatgctg gaacacaagc
     8461 atgtctagct ttaaaagaag agggataccg agttattctt gtaaattcaa atccagcgac
     8521 aatcatgact gataaggaaa ttgcggataa agtatatatc gaaccgttaa ctcataattt
     8581 tatagcgcga attatacgta aagagcaacc tgacgcttta cttccaactt taggtggtca
     8641 aacaggttta aacatggcga ttcaactaca cgaaagtggt gtgcttcaag ataataacgt
     8701 ccaattatta ggaactgagc taacatcaat tcaacaagca gaagaccgtg aaatgtttag
     8761 aacattaatg aatgatttaa acgttcctgt gccagagagt gacattgtaa atacagtaga
     8821 gcaagccttt aaattcaaag agcaagtggg atacccgcta attgttagac cagcatttac
     8881 gatgggtggt accggaggcg gtatttgtca taatgatgaa gaattacatg aaatcgtttc
     8941 aaatggtctt cattatagtc cagcaacgca atgtttatta gaaaaatcta tcgcaggttt
     9001 taaagaaatc gaatacgaag taatgcgtga taaaaacgat aatgccatcg ttgtatgtaa
     9061 catggaaaat attgatccag ttggtattca tacaggcgat tcaattgttg tagctcctag
     9121 ccaaacatta tcggatgttg agtatcaaat gttacgtgat gtttcattaa aagttattcg
     9181 agcattaggt atcgaaggtg gttgtaatgt tcaattagca ttagatcccc attcattcga
     9241 ttattatatt atagaagtaa atccgcgtgt atcacgttca tcagcgttag cttcaaaagc
     9301 aacaggatat cctattgcaa aattagctgc taaaatcgcg gttggtctaa cattagatga
     9361 aatgttaaat ccaattacag gaacatctta tgcagcgttt gaaccaactt tagactatgt
     9421 gatttcaaaa ataccaagat ttccttttga taaatttgaa aaaggagaac gagagcttgg
     9481 cacacaaatg aaagcaacag gtgaagttat ggccattggt cgaacttacg aagaatcatt
     9541 gttaaaagca attcgatcac ttgagtatgg tgtgcatcac ttaggattac caaatggtga
     9601 aagcttcgat cttgattata ttaaagaacg tatttcacac caagatgatg aacgattatt
     9661 tttcatcggc gaagcaatta gaagaggcac aacattagaa gaaattcata atatgactca
     9721 gatagattac ttcttcttac acaagttcca aaacattatt gatattgagc atcaattaaa
     9781 agagcatcaa ggtgatttag aatatcttaa atatgcaaaa gattatggat ttagtgataa
     9841 aacaatagcg catcgcttta atatgacgga agaagaagta tatcaattgc gtatggaaaa
     9901 tgatattaaa cctgtttaca agatggttga tacttgcgca gctgaatttg aatcttcaac
     9961 accatattat tatggtacat acgaaactga aaatgaatcc atagttactg acaaagaaaa
    10021 aatcttagta ttaggctctg gaccaattcg aatcggccaa ggtgtagaat ttgactatgc
    10081 gacagttcac gccgtttggg caattcaaaa agcagggtac gaagcgataa ttgtgaataa
    10141 caatccagaa acagtttcaa cagacttctc aatttctgac aaattatact ttgaaccttt
    10201 aactgaagaa gatgtgatga atatcattaa tttagaaaaa cctaaaggtg tcgttgtaca
    10261 atttggagga caaacagcga ttaatttagc agacaaattg gctaaacatg gtgttaaaat
    10321 acttggtact tcactagaaa atctaaatcg tgctgaagat agaaaagaat ttgaagcact
    10381 attaagaaaa attaacgtgc cacagccaca agggaaaaca gctacatcac ctgaggaagc
    10441 attagcgaat gctgcagaaa tcggatatcc ggttgtagta agaccttctt atgtattagg
    10501 tggtcgcgca atggaaattg tagacaatga caaagagtta gaaaactata tgacccaggc
    10561 tgtaaaagcg agtccggaac atccggtatt agtcgataga tatttaactg gtaaagaaat
    10621 tgaagttgat gcgatttgtg atggagaaac ggtcattatt ccaggaatta tggaacatat
    10681 tgaacgtgct ggtgtgcata gtggtgactc aatcgctgta tatccgccac aaactttgac
    10741 agaagacgag ttagcaacgc ttgaggacta tactataaaa ttagctaaag gtttaaacat
    10801 cattggctta atcaacattc aattcgttat agctcacgat ggtgtgtatg ttttagaagt
    10861 aaatccacgt tctagtagaa cggtaccatt cttaagtaaa attactgata ttccaatggc
    10921 acaattagct atgcgagcaa tcattgggga aaaactaaca gatatgggtt atcaagaagg
    10981 ggttcaacca tatgctgagg gtgtatttgt gaaagcaccg gtatttagtt ttaataaatt
    11041 gaaaaatgtt gatattactt taggacctga aatgaagtct acaggtgaag taatggggaa
    11101 agatacaaca ttagaaaagg cgttattcaa agggttaaca ggtagtggtg ttgaagttaa
    11161 agatcacggt acagtattaa tgactgtcag tgacaaagat aaagaggaag ttgttaaatt
    11221 ggcacaatgc ttaaatgaag ttggctataa aattttagca acgtctggaa cagctaataa
    11281 attagctgag tatgacatac ctgcagaagt agtaggcaaa attggtggcg aaaatgattt
    11341 attaacacgt attcaaaatg gtgatgttca aatcgttata aatacaatga ctaaaggtaa
    11401 agaagtagaa agagatggct tccaaattag acgtactaca gttgaaaatg gtattccatg
    11461 tttgacatct ttagatacag ctaatgcctt aacgaatgta attgaaagta tgacatttac
    11521 aatgcgtcaa atgtaaatca atcaaactgt atcggtgggg ctgtaattaa ccatttactt
    11581 aaagaagttt atattacagc ctcattattt tagtgaattt cttaacgtaa agggagacat
    11641 atatgatgaa agatttacca attattgcat tagattttga atcaaaagaa aaagtaaatc
    11701 aatttttaga tttatttgat gaatcattat tcgtaaaagt aggtatggaa cttttttatc
    11761 aagaaggtcc tcaattaatt aatgagataa aagaaagagg ccatgatgta tttttagatt
    11821 taaaactgca tgatattccc aatacagttg gtaaagcgat ggaaggacta gctaaattga
    11881 atgttgatct ggtaaatgtt catgctgctg gtggcgtaaa aatgatgtct gaggccatta
    11941 aaggattaag aaaacataat caacatacaa aaattattgc agtaacacag cttacgtcaa
    12001 caacagaaga catgttacga cacgaacaaa atatacaaac atcgattgaa gaggccgttt
    12061 taaattatgc caagttagca aatgcagctg gtttagatgg cgttgtttgt tcacctcttg
    12121 aaagtcgtat gttgactgaa aagttaggta catcattttt aaaagtaaca ccaggcatta
    12181 gacctaaagg tgcatctcaa gatgaccaac accgtattac gacaccggaa gaagcaagac
    12241 agcttggttc gacgcatatt gtagtcggta gaccgattac acaaagtgac aatccagtcg
    12301 aaagttatca taaaattaaa gaaagttggt tagtataatg gctaaagaaa ttgcaaaatc
    12361 attattagat attgaagctg taacattatc accaaatgat ttatatacat ggagttcagg
    12421 tattaaatca ccgatttact gtgataaccg tgttacgtta ggttatcctt tagttcgagg
    12481 cgcaatccgc gatggtttaa ttaacttaat taaagaacac tttcctgaag tagaagttat
    12541 ttctggtact gcaacagctg gtattccaca tgcagctttt attgctgaaa aattaaaatt
    12601 accaatgaat tatgttcgtt catcaaataa gagtcatggt aagcaaaatc aaattgaagg
    12661 tgctaaaagt gaaggtaaaa aagtagttgt gatagaagat ttaatttcga cagggggatc
    12721 ttcagtcaca gcagttgaag ccttaaaaca agcaggtgca gaagtattag gtgttgttgc
    12781 tatctttact tacggtttga aaaaagcaga tgatacattt agcaatattc aactaccttt
    12841 ttacacttta agtgattaca atgaattaat tgaagtagct gaaaatgaag gtaaaatttc
    12901 tagtgaagat atccaaacat tagttgaatg gagagacaac ttagcataat atagacacta
    12961 gaaggaggaa ttcaacaaat gaatgacaaa acatctaatg atttatatgg gaagataaaa
    13021 cattgtaacg aatttatcaa tcattcaaat gattccaatc tatctagtag tcacgatgtc
    13081 gacgaaagtt caacgaagca aaaacatata aaaaataaaa caactataaa tcataatgat
    13141 gatttattta aacatgtaaa ggatatatta cgtaaacaag gacaaattta aaaacaaaaa
    13201 agctatagtg tttaatatca ggggctgctt aaccgcatcc gaatattaca aacactatag
    13261 cttttattat ataaaaaatt tattagcgga taattaactt tattaaaatc caaccaatta
    13321 agaatacgag taataggact aatactggaa tcacataatg taacataacg tccctccttt
    13381 aacttaattt taattgtaat caaatttgac aataagtcaa aacattaata cctatgataa
    13441 gtatcattta ttaacatatg tatcatattt ttaatcttgc gtaattttta tcgttaacta
    13501 tgtgatttaa tcaacaggcc aataagacat ttgtcattcg tgcacataac ggtacgacgc
    13561 gcttttgtca ttttaattat gttaagataa tagtagttat agaagttcaa tctataggag
    13621 gcatagcatg gatattccaa aaattacgac atttttaatg tttaataacc aagctgaaga
    13681 agctgttaaa ctatacacaa gcttatttga agatagtgag attataacaa tggctaagta
    13741 tggtgaaaat ggacctggtg atcctgggac tgtacaacac tcaatattta cattaaatgg
    13801 acaagtattc atggcgattg atgctaatag tggcacagaa ttaccaatga atcctgcgat
    13861 ttcattattt gttacagtaa aagatactat tgaaatggaa cgactattta atggattaaa
    13921 agatgaaggt gccattttaa tgccaaaaac gaatatgcca ccatacagag agtttgcttg
    13981 ggttcaagat aagtttggag taagttttca attagcatta cctgagtaaa aggattcgta
    14041 cagcgttgaa tgataaagaa acactttttc ttatgcatgc gttttaccta tgtattccta
    14101 ttttaagtac acattatcat tttagtttca ctcattttta aaatcacaaa gataattttt
    14161 aacttaaatg atgatacgtt attaaaaaac acgatacttc gtttcaatga acgcatcaaa
    14221 ttaaaaataa acacctcgca aaagaaagta ggcctttctt atggtgaggt gtttatttgt
    14281 cgacaacttg attatgattg tttcattttt tgaatatgtt cataatcagg tgtagcatac
    14341 aaagtttttt gattgtcata tgttacaaac ccaggctttg cacctgatgg tttgtgcaca
    14401 tttttaatta acgtgtaatc aacaggtatt tgtccagaat taccagcttt tgaaaagtat
    14461 cctgctaaca tagccgcttc cttgattgtc gtatcacttg gtgcatcatt aaatataacg
    14521 acatgtgaac caggaatatc ttttgtgtgt aaccatgtgt gagttttttt agctttttta
    14581 tttgttaaat aatcattttg cttgttattc ttgccaacat atatatcgtc gccatcagtt
    14641 gatacataat gttgtaattg aatctgcgct ttctttttct tagtttgatt tttacgctgt
    14701 ttcataaagc cttgttctgc taattcatct ctaatttcat caatgtcatg gacagaaata
    14761 tgatgtaatt gttgttcgat tgttgaaaaa taatctatat tgtctttcgt caattgaatt
    14821 tgatgttgta attcacgttc tctcgttttc atacgattat attgtttata ataatattga
    14881 gcatttgcag atggggattt tgtaggattt aaaggaatga caacttcttc attcgtataa
    14941 taattcaatg ccgtcacttc tttatcgcct tgcttaattc gatatatatt agcagtaatc
    15001 aattcaccat ataactgttc agtatcttta tttttagact gctcatattc ttcaatcaac
    15061 ttcgctaatt tattttgata tttgtgcaac tgctgttgaa caaatcgaac taaatcattc
    15121 gcacgttgtt taacgcgttc ccgttcgcca cgcgcatcat aaaaacgatc aagtaaatca
    15181 tttaatgaat cgtacgtaac tgtatcatca ttaaattgat ttaactttat aaaatagaaa
    15241 tcctctttac ctgtttcatg atttttatga aaaataggag taggtggtaa cttggtttct
    15301 gccattactt cgtcgaatgc ttctggtaaa gttgatgaag tcataaattg acgacgatta
    15361 acgatttcat tcgtaattaa agggctaaac ccttcaaact gattcaataa ttgtttagca
    15421 atattacctg cgttaaaatc gatatatttc aacacctctg cacctgtaat atcatacgga
    15481 ttcattttgt gctgagtagg tggtgcttca taattaaatc ctggcattac tgttcgatag
    15541 tgattcgtat ttggtgttaa gtgtttaaat ccttcaatta ttttacgatt ttcatctact
    15601 aaaattaagt tactatgttt acccataatc tcaaggatga cagtgcggta aatagtatcg
    15661 ccaatttcat ctttactctt tatatcgatt tcaatgcgac gatcattacc aatttgctta
    15721 atcgattcga taataccacc ttctaagtgt tttctaaaaa cacgcgcaaa catgggtgga
    15781 ttaaatggat tatcatattt tttagtagtc aattgtaatc ttgaaaagtt tggatggatt
    15841 gacaataaca attgatggtt ttgtctattt tgacgtacaa ccattagtat cgtgtcatta
    15901 tcaggttgat tgattttgtg aacgcgtcct gttgttaaaa attgtagaga ctcaaccatt
    15961 ttctttgtaa ataagccatc ataagccata atttatcttc caccttcttc atatcattcg
    16021 tttcatttta acatgattgt aacttttcgg tatagttatt gaatcataca ttatgccaca
    16081 catttaacat tatctaaaag ttcgcaattc aatttattta attaaaatta atcgtgcaat
    16141 ataacgatat tgttagacct aatagaaatt atggcatgca atttcaaata tgctatacaa
    16201 tataaagaac aatgtgatat catatttaaa taatagaaga ttagcttaga gaggtcgtaa
    16261 ggcatggata atgaaaaagg attgttaatc gttttatcag gaccatctgg agtaggtaaa
    16321 ggtactgtta gaaaacgaat atttgaagat ccaagtacat catataagta ttctatttca
    16381 atgacaacac gtcaaatgcg tgaaggtgaa gttgatggcg tagattactt ttttaaaact
    16441 agggatgcgt ttgaagcttt aattaaagat gaccaattta tagaatatgc tgaatatgta
    16501 ggcaactatt atggtacacc agttcaatat gttaaagata caatggacga aggtcatgat
    16561 gtatttttag aaattgaagt agaaggtgca aagcaagtta gaaagaaatt tccagatgcg
    16621 ttatttattt tcttagcacc tccaagttta gatcacttga gagagcgatt agtaggtaga
    16681 ggaacagaat ctgatgagaa aatacaaagt cgtattaacg aagcacgtaa agaagtcgaa
    16741 atgatgaatt tatacgatta cgttgtagtt aatgatgaag tagaacttgc gaagaataga
    16801 attcaatgta ttgtagaagc tgagcactta aaaagagagc gcgtagaagc taagtataga
    16861 aaaatgattt tggaggctaa aaaataatgt taaatccacc attaaaccaa ttaacgtcac
    16921 aaattaaatc aaagtattta attgcaacaa ctgcagcgaa aagagcgcgt gaaattgatg
    16981 aacaacctga aactgaatta ttaagtgaat atcattcatt taaaccagtt ggtagagcgt
    17041 tagaagaaat tgctgacggt aaaattcgcc ctgttatttc aagcgattat tatggtaaag
    17101 aatagtttcg accattaaaa atatgtgaag caagacgtat atcaaatacg tcaaggcata
    17161 tccaaatcaa acgcacctat gtaggtgtgt ttttttagtt tatccaatga ttgatgttat
    17221 aataatacta aatttgtatc tataaaaaag taatgagcat ttgtgcgcat atgatgatgt
    17281 aaagcgtaaa tgtcatagtg atatttaagg gagattgaat atgaagaaaa tattgttagc
    17341 cgttacaggt ggcattgcgg catataaagc gattgatttg acaagtaagt taacacaatc
    17401 tgggtatgaa gttcgcgtta tgttaacgaa tcacgcacaa aaatttgtga caccattagc
    17461 atttcaagca ataagtcgaa atgctgttta tacagatact tttatagaag aaaatccttc
    17521 agaaatacag catatagcat taggtgattg ggcagatgca atcattgttg cacctgcaac
    17581 ggcaaataca attgcaaaat tgagtgtagg tattgctgat gatttggtga catcaacgtt
    17641 gctagcaaca gagacaccga aatttattgc gcctgctatg aatgtgcata tgtatgaaaa
    17701 taaacgtacg cagcaaaata ttaatatttt aaaagaagat gggtatcatt ttatcgaacc
    17761 aggaagcgga tttttagcat gtggttatgt tgctaaagga cgtatggaag aaccgcttca
    17821 aatcgtttct gttattgatg ctcattttca aaatagtaat cgtttagcta atagttcatt
    17881 tcaagataaa cgcgcattgg ttacagccgg accaactatt gaagttatcg atccagtcag
    17941 atttgtatcc aatcgttctt ctggaaaaat gggttatgca atagctgaag cattgcgaaa
    18001 tcgaggagct atcgtgacat tagttgcggg gcctacaaca ctagaggatc caaaagatat
    18061 tgaagttatt catgttcaaa gtgctgaaga aatgtttgaa caagtgacaa gccgatttga
    18121 cgaacaagat attgttgtaa aagcagcagc cgtatctgac tatacaccgg ttgatgtatt
    18181 agaacataag atgaaaaagc aggacggtga tttgtcagta tcttttaaac gtactaaaga
    18241 cattcttaaa tatttaggtg aacataaaac atcacagtat ttaataggct ttgcagcaga
    18301 gactgaagat attgaaaatt atgcacaaca aaaattacgc aagaaaaatg cagatgtgat
    18361 tatttcaaat aatgttggag atatgtctat cggatttagt tctgatgata atgaattgac
    18421 aatgcatttt aaaaataatg aaaaagtaaa tatcaagaaa ggaaaaaaag tagtattagc
    18481 tgcacaaatt ttagatgaac tagaaactag gtggcaataa tgatagcgaa agtcatagtc
    18541 gatgtcgcgt cgaagagcgt tgactataaa tttgattata taattcccga acaactcgaa
    18601 tctgtcatcc aacctggtgt gcgtgtgatt gtaccttttg gaccaagaac gattcaaggt
    18661 tatgtaatgg aagtaacagc agaacctgat gcacaacttg acgtttcgaa gttaaaaaaa
    18721 atcatagaag tgaaagatat acaaccagaa ttaacatcag aattaatagc tttaagtgag
    18781 tggatgggtt caactcatgt cattaaacgt atttctatgc tagaagtgat gcttccgagt
    18841 gctattaaag cgaagtataa aaaagcattt aagatgaaag atgacataga ggtaccttca
    18901 actttattac aaaaatttga taagcatggt tactattatt ataaagatgc gcaaaaaaat
    18961 aatgatattc aattgcttat gaagttgtta aaagatgata tcgttgaaga aagaacgatt
    19021 ctcacacaaa atataactaa aaaaaccaag cgtgctgttc gtgtcattga agggtatcat
    19081 cctgatgaag tattagctaa gttggagaaa gttattaaac aatatgattt gtatgcttac
    19141 ttgtctgaag aacaacataa aacaatattt ttaactgata ttgaggatat gggcttttca
    19201 aaatctagtt tagatggact tatcaaaaaa ggttatgttg aaaaatatga cgcggttgtt
    19261 gaaagagacc catttaaaga tcgtgttttc gaacaagaat caaaacagca attaacagaa
    19321 gaccaatata aagcatatga agcgattaaa gctaaaattg taagccaaga gcaagaaaca
    19381 tttttacttc atggtgtgac gggatcaggt aaaacagaag tatatttaca aacgatagaa
    19441 gatgttttaa gtcaaggaaa acaggcgatg atgttagttc ctgaaatcgc tctaacaccg
    19501 caaatggttt tacgcttcaa acgtcgattt ggtgatgacg ttgctgtatt acattctggc
    19561 ttatctaatg gggaacgtta tgatgagtgg caaaaaatta gggatggtcg tgcgagagta
    19621 agtgttggtg caaggtcaag tgtgttcgca cctttcaaaa atttagggtt aatcatcatt
    19681 gatgaagaac atgaatctac atataagcaa gaagattatc cgagatatca cgctagagaa
    19741 attgcccaat ggcgaagtga atatcatcac tgcccagtca ttttaggaag tgcaacacca
    19801 tgtcttgaaa gttatgcacg agctgaaaaa ggtgtttatc atttgctatc attaccaaac
    19861 agagtgaacc aacaagcttt acctgaaatt gatatcgtag acatgcgtga agaattgagt
    19921 gaaggtaatc ggtcaatgtt ttcaaaagat ttacgtgaag ccatacaatt aagattagat
    19981 cgacaggaac aagttgtttt atttttaaat cgacgtggtt atgcatcatt tatgttatgt
    20041 cgggattgtg gatatgtacc gcaatgccca aactgtgata tttcattaac gtatcataaa
    20101 acgacagact tattaaaatg tcactattgt ggttaccaag agacaccacc gaatcaatgt
    20161 ccaaattgtg agagtgaaca cattcgacaa gtaggtactg gtactcagaa agttgaagaa
    20221 ctattgcaac aagaatttga agatgcgcgc ataattagga tggatgtaga tacaacctca
    20281 aagaaaggtg cacatgaaaa gttattgact gaattcgaaa aaggtaacgg tgacatttta
    20341 ctaggtactc agatgattgc gaaaggatta gattatccaa acattacttt agttggtgtg
    20401 ctgaatgcag atacaatgtt aaatttacct gattttcggg cgagcgaacg tacttatcaa
    20461 ctattaacgc aagtggctgg tagagctggt cgtcatgaaa aggcaggtca agtcatcatt
    20521 caaacgtata atccagatca ttattcaata ttggatgttc aaaaaaatga ttatttaaca
    20581 ttttatcgtc aggaaatgga atatcgtaaa ttaggaaagt atccaccgta ttattatttg
    20641 attaatttca caatctcaca taaagaaatg aagaaggtta tggaagcatc gcaacatgtt
    20701 cataaaattt tattacagca tttaacagaa aaagcgcttg tactaggtcc atctccggca
    20761 gcacttgcga gaatcaacaa tgaatttaga ttccaaattt tagtgaaata taaaagtgaa
    20821 cctggattat tacaagccat tcagttttta gatgactatt accatgaaaa atttataaaa
    20881 gaaaaattag cgttgaagat tgatattgat ccacagatga tgatgtaatt ttttgactaa
    20941 aacactttaa taccaatgtt tcctaagagt ttgaaaggtc ttgtttttac tttaagggca
    21001 gaaaaagggc agaatataca actgttaacc atttaaatta gcagtttata ttctgtcttt
    21061 ttatatggct ttataactta cgtgattttg atttgattag ggatttatta gtattttcat
    21121 ttacgaattc tgattttaat gtaagtgcca tacttactga taaataaaag tactaatgat
    21181 tatgcaaact atttttaaat ggcaaattga aatggtgcaa attttacact tgtaa
//
LOCUS       DATTQF010000029        21214 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.029, whole genome shotgun sequence.
ACCESSION   DATTQF010000029 DATTQF010000000
VERSION     DATTQF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.029"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1150)
                     /gene="hflX"
                     /locus_tag="VB241_000991"
     CDS             complement(<1..1150)
                     /gene="hflX"
                     /locus_tag="VB241_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP1244295.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKLQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDEAIATNNADR"
     gene            1262..1738
                     /locus_tag="VB241_000992"
     CDS             1262..1738
                     /locus_tag="VB241_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP1244296.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(1960..2193)
                     /gene="hfq"
                     /locus_tag="VB241_000993"
     CDS             complement(1960..2193)
                     /gene="hfq"
                     /locus_tag="VB241_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEP1244297.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEAQESTESEE"
     gene            complement(2208..3143)
                     /gene="miaA"
                     /locus_tag="VB241_000994"
     CDS             complement(2208..3143)
                     /gene="miaA"
                     /locus_tag="VB241_000994"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP1244298.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIGILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSVVKQKLSALEHLDNQQLHEYLAQFDEASAKNI
                     HPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(3161..4075)
                     /locus_tag="VB241_000995"
     CDS             complement(3161..4075)
                     /locus_tag="VB241_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1244299.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKKLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(4225..5898)
                     /locus_tag="VB241_000996"
     CDS             complement(4225..5898)
                     /locus_tag="VB241_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEP1244300.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVNVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(6056..7552)
                     /gene="glpK"
                     /locus_tag="VB241_000997"
     CDS             complement(6056..7552)
                     /gene="glpK"
                     /locus_tag="VB241_000997"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP1244301.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(7682..8500)
                     /locus_tag="VB241_000998"
     CDS             complement(7682..8500)
                     /locus_tag="VB241_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP1244302.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVYAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(8975..9517)
                     /locus_tag="VB241_000999"
     CDS             complement(8975..9517)
                     /locus_tag="VB241_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEP1244303.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            complement(9523..11532)
                     /gene="mutL"
                     /locus_tag="VB241_001000"
     CDS             complement(9523..11532)
                     /gene="mutL"
                     /locus_tag="VB241_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP1244304.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENKQEKTFSSEESNSKPFMAENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEIQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            complement(11545..14163)
                     /gene="mutS"
                     /locus_tag="VB241_001001"
     CDS             complement(11545..14163)
                     /gene="mutS"
                     /locus_tag="VB241_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP1244305.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDHLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDAVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQDTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNMQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQHQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENSNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            complement(14466..14957)
                     /gene="thiW"
                     /locus_tag="VB241_001002"
     CDS             complement(14466..14957)
                     /gene="thiW"
                     /locus_tag="VB241_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEP1244306.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLLTLKKRGILQR
                     FIK"
     gene            complement(14984..15349)
                     /locus_tag="VB241_001003"
     CDS             complement(14984..15349)
                     /locus_tag="VB241_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HEP1244307.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            complement(15350..16894)
                     /gene="miaB"
                     /locus_tag="VB241_001004"
     CDS             complement(15350..16894)
                     /gene="miaB"
                     /locus_tag="VB241_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEP1244308.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIECDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            complement(17028..17321)
                     /locus_tag="VB241_001005"
     CDS             complement(17028..17321)
                     /locus_tag="VB241_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEP1244309.1"
                     /translation="MKDTLMSVQIIPKTPDNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            complement(17416..18282)
                     /locus_tag="VB241_001006"
     CDS             complement(17416..18282)
                     /locus_tag="VB241_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEP1244310.1"
                     /translation="MPTFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEQVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            complement(18283..20043)
                     /locus_tag="VB241_001007"
     CDS             complement(18283..20043)
                     /locus_tag="VB241_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEP1244311.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLSEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            complement(20183..20980)
                     /locus_tag="VB241_001008"
     CDS             complement(20183..20980)
                     /locus_tag="VB241_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEP1244312.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IKINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
BASE COUNT         6425 a         4024 c         2883 g         7882 t
ORIGIN      
        1 atcgatctgc attattcgtc gcaattgcct catcataata agttaattgc cttttgattt
       61 catcaataaa taaagtctta acaagtaatt tatcaccatc atttttagat gaaacaaaaa
      121 cattaggcaa atcacttgct ggacgatttg atgcatgatc acataagtcc tttttattaa
      181 aaataactat ttgagaaata tgactcatat ctaattgttt gattaaatca ttaactgtgt
      241 catactgcgt acggtattca ggatggctac tatctacgac atgtactaat aaatctgcac
      301 ctttagcctc ttctaaagtt gatttaaaag ctgcaattaa cgtcgtaggt agtttctgta
      361 taaaaccaac agtatctgaa ataattaaat taaatccatc atttatttga atttgtcgtg
      421 ttttaggatc taacgttgca aataattgat ctttttcata cgtctcttca tttgctaaaa
      481 cattaaacca tgatgattta ccagcatttg tataaccaac taaagctact tgaaacacct
      541 gattttgatt tcttttattt cgatatcttt cgcgatgttc ttctaccgtt cgcaattgaa
      601 gttttatttc attcatacga gttcgaatat gtctacgatc catctctaac ttcgtttcac
      661 cagggcctct agttccaata ccgccaccta aacgagaaag acttttacca tggccttgca
      721 atctaggtaa taaataatca agttgtgcta gctctacttg caatttacct tctttacttc
      781 ttgctcttaa tgcaaatatt tcaagaatca actgagttct atcaataatt tttacaccta
      841 aagcttcatt tagtgatttg gattgtgcag tcgttaattc atcatttgtg atgactacat
      901 caatatcttt gaactcaata aatgcttgaa tttcttcaat tttaccttta ccaacataat
      961 atttgcgatc tacacgatct ctgttttgag taatttgacc caacacttca agttggcaag
     1021 tctctgataa agatgataat tcctccattg tagactcaaa attaaattgc ttatcatctt
     1081 gagcatgtac accgactaat acagcttttt ctagtttatt tttagtatca tgaatttgtt
     1141 gctgagccat gttatacatc tccttaataa aatcctactc aaagtattct atcatagagt
     1201 tttagtaaag actattacac gattttatgg tacattttaa gtaaatcgaa aggatgattc
     1261 aatggagaca atttatgatt ttgtagtaga aacaaataaa ggtgttactt acaaattaga
     1321 tgcatataaa ggtgacgtta tgctaattgt caatacagct agcgaatgtg gctttacttc
     1381 acaattcgaa ggtttgcaat cattatacga gaaatacaaa gatcaagggt ttgtaattct
     1441 aggtttccct tgtaatcaat ttggtggcca agaacctggt tcaggcgagg aagcagctca
     1501 aaactgtaaa ttaaactatg gtgttacctt ccctatgcat caaaaaatag acgtaaaagg
     1561 ggaacatcaa ttacctttat ttagatattt gactgcggca caacatggat tctttaatga
     1621 gaaaatcaaa tggaatttca ctaagttttt agtggatcgt gaaggtaacg ttgttaaacg
     1681 atttgcacct caaaaaaaac ctgttcaaat cgaaagagaa atagaaaagt tattataaaa
     1741 tatactagct catagcaaat aaaacgcttc atataaagtc gtatcatata ttgactcgac
     1801 ctttatatga agcgttttta ttacaaacat tcctctattg tatcttgtca tttgagcatc
     1861 atttaattga tgtcacatat gactttattt ctatatctat ttatcatact tcaaacgtta
     1921 tgtacacgag tcattaaatc gtgcccatat gattacaact tattcttcac tttcagtaga
     1981 ttcttgagct tcagtttcta ctgtataagt gctgatcgca tgtttgtaaa tcaagtgttg
     2041 tttgccttga gaatttaagc ttacgacata cttgtcgtat tcttcaataa cacctttcat
     2101 ttggaaaccg tttagaaaga atacagttac ttcagtttgg tttgctttaa aattctctag
     2161 tgctttgtct tggatgtttt cgtttgcaat catctgtcgg actcctttta cttaatctgg
     2221 gttgtaatct catctaacat catttgaagt gacatatttt ctttatctaa ccaatgaaca
     2281 ctcattttat tcttgaacca tgtcatttgt cgttttgcat attggcgtga atgttgcttt
     2341 aaatcattga cagcatcttc ataaatcatt tgtccgttaa tcacaggtat taattcttta
     2401 tatccaatag cttgcatact ttggcaagat tcatagcctt gttcaacaag ttgttgcact
     2461 tctctaaata atccgtgatc caacataata tcaacacgtt tatttattct tgaatataat
     2521 gttttacgcg acatttcaat ccctattaat aatgtatcat aattttcagt aaattgttgc
     2581 actttcttac gattacttaa aagttttttt gttttaaaat agtattcgat agcgcgcaat
     2641 actctctgtc gattgtttgg atgaatattt ttggcagaag cctcgtcaaa ttgagctaaa
     2701 tattcgtgta gttgctgatt atctaaatgt tctaatgcag ataacttttg tttaactacc
     2761 gataattgtg caggtgtaac tgtttcatct tctaattcat aattatatat taatgattga
     2821 atatataagc ctgttccacc tgctatgatt ggaactttac ctctattcgt tatatcagta
     2881 attaaatctt ctgctaatcg cttgaattca tatgctgaaa atgtatcatc aggattcaag
     2941 ataccaatta aatgatgtgg aataccatcc atttcttcag gtgttacttt tgcagttcca
     3001 atattcatat gtttgtagac ttgcatagag tcaccgctta tgatttcacc attgatacgc
     3061 ttcgcgagtt cgatgctaag ctctgtttta cctgaagcag ttggccccac aattactaca
     3121 ataaaaggct tatttttatt catatttaat tccactttct ttatttttta taaaattgat
     3181 tcaaccattt aaacaagtct tcccaaattt tgtcatgatc tttttcaaat aacacttcat
     3241 gacgtttatg atgataaaga ttaactttaa catttttaat gccggctttt ctatatatat
     3301 tcgccaattt taaaatccct ttaccataat cacctaaagg atcgtcatat cctgaaacta
     3361 ataatatagg catggcatga tttaactttt ttatattttt taattgtgat gtatgtagca
     3421 tataatatgc tgtttgatat aataattgat ttgacacatt aaatccacta tatggatctt
     3481 taataaagtt gtctacttca gttggattac tagaaatcca atcactttgt gtacgtaatg
     3541 gacgtatttt tttattaaaa ctatttgata ctaactggtt aacccattca acccgtttct
     3601 cggcaccata aatttttgta atcagttgta acaccttaac ggttggtaat cctttccata
     3661 atggatacat accagtacca cttaaaatta gaccatcaac atattgcgga tatgtttcta
     3721 caaacaatct agctataacg gatcccattg aatgtccgat tataatgtat ggtttatcaa
     3781 cattgcctct cactgtttgc gctacttcaa aggcatcacc gataactcgt ttcatatcat
     3841 cgtaatgccc tcttgttgat tcatcaatat taataccatg tcctcgatga ttatgacgta
     3901 tcacatcgaa gccatgctta gaaagtgcat gtgctaattt atcgtaacgt tccatatgtt
     3961 cagccatacc atgaaatata tgaataattc cttttgaatc tacatctgta aaattcactt
     4021 taacttcaat attcgtatta tcttgtacag ttaatttaaa ttgtttttca gtcatcatct
     4081 cacaccttta ataacaacgt ttttatattc gcttttatta ttatcataca tttagtgatg
     4141 catagtttca attgactaat gtatctcata taaaaaaact gatgccctta aaacaggaca
     4201 ccagtttatg attgtacaaa ttaattattc tttaactgct ggttgattat ttccatgttg
     4261 cgcttcttta attgcttgct caacttcttc agtataagcc tcaatttgag ctggagaata
     4321 gtctaacata tcagccataa tatcgatgat actatcttta taatctaata catctttaat
     4381 attgaagtac attttaccag aacgacgaac taagaaatcg ttaggtttgt ataccatttc
     4441 ttgttgaata ctataaacaa gttctacata aatttctaat ggtaacttgc tgtcatggta
     4501 ttgagatgtt tgcgcaatgt tgaacaattc atcaacattt gaaccatatt tagatgctaa
     4561 acgtctcgca acatcttcat caatgccgaa tcctttagct acatctactt tttgctctac
     4621 aaacgcatca aagttcttgc taccacctac atcgccacca gaaattgcca gaccttttgt
     4681 attacatgga ctaaatgtta aaccatagtc ttttttcaag cgtttagata ctaaatcaac
     4741 aatgtcttga gccatgtgac gatagcctgt taatttacca cctgcaatag ttaataaacc
     4801 tgatttacct tcccaaattt catccttacg agagatttca gaagggtctt tgccctcttc
     4861 gtaaattaat ggtctaattc ctgcccatgt tgattcaata tcttcatctg taacattaac
     4921 actagggaac atgtaattaa tcgcatcgat taaataatct ctgtcttctt gtgtagttaa
     4981 tggtgaagat ttgatattgt catagaatgt atctgtagta cctacatacg cttttccttc
     5041 acgcgggatt gcaaaaatca ttcttccatc tttttcagta tcaaagtata ctgcttgacc
     5101 taatgggaat tttgattgat caataacaac atgtacacct ttagttaaac gtaatttttt
     5161 attattgcgt gcataatcac cacttctaac atcatcaacc catggacctg ctgcattaac
     5221 cactttttta gccttaattg tataattttc attagttaat ttatctataa cattaacacc
     5281 atttacttgt tgatttttat cataagtgaa gtgttctgac ttagtatagt tgataatttc
     5341 tgcgcctttt tcggcagcac gcttcataac ttcaatagtt aaacgcgcat cgtcagtacg
     5401 atattcaaca tagtaaccgc cgccttttag accttctttt ttaactaatg gttctttagc
     5461 taaagtttct tttttagata acattttttt acgttcagac ttcttaacac ctgctaaacg
     5521 atcatacatt cctaaaccaa ttgatgttga gaatttacca aatgttccac ctttatgcat
     5581 tggtaaaagc atccactctg gagtcgtaac atgaggccca ttttcataaa caatcgcacg
     5641 ttctttacca gtttcggcaa ctactcctat ttggaattgt tttaagtaac gtaaaccacc
     5701 atggactaat tttgtagatc ttgagcttgt tccttgtgca aagtcttgca tttcaactaa
     5761 tgcaactttc attcctcttt cactcgcgtc tagtgcaata cctgcacctg taataccgcc
     5821 accaataatt actaaatcat attcatcatt tcttaaattc tttttaatat gttctctctt
     5881 aaaagtagac aatgccattc caaaacgcct cctaaaaaat gtaaaaacga gagacttgtc
     5941 ccacataaac aatccttcaa ttgtacatgt tcgaacaaat ctctcgtttc tcgattcaaa
     6001 tattaactta aattgattgt atcacaatgt ttatacaaaa gtctaatcta caagtttatt
     6061 ctgttttaaa aacttgtgtt gcttcaacag cttttttcca acctctatat aatttttctc
     6121 tttcgccttc atccattttc ggatcgaatt tttcttctaa tttccagttt ttagcgatat
     6181 catctttact ctcccagaat ccaactgcta aacctgctaa atatgcagca cctaaagctg
     6241 tagtttcttg aatttcaggt ctttcaacag aagtattaac aatgtctgct tggaactgca
     6301 taataaagtt attcttaact gcaccaccat cgacacgtaa actttggaca tcgataccag
     6361 agtcttttga cattgcttcc ataacgtcac gagtttggta acataatgat tctaatgttg
     6421 cacggataaa gtgctctttt tcagttccac gtgttaaacc gaaaatcgca ccacgtgctt
     6481 cagaatccca atatggtgtt cctaaaccta caaaagctgg aacaacataa acaccctcag
     6541 tagagtcaac tcgtgtcgca taactttctg attgtggtgc tgaattaatc attcttaatc
     6601 catcacgtaa ccattggatt gctgaacccg aaacaaagat ggaaccttca agcgcataat
     6661 ttacttttcc atcaatacca taagcaattg ttgttaataa accactttca gatttaaccg
     6721 ctttgtcacc tgtattcatt aacatgaagc caccagttcc atatgtgttt ttcacgtcac
     6781 cacgttcgaa gcaagcttgt ccaaataatg ctgcttgttg atcaccagct acaccagcaa
     6841 ttggtacttc ttgaccatag aagtggtaat caattgtctt accatatact tcacttgaag
     6901 gtttaacttc tggcaacata tttttaggta ctgtaagtag ttctaataac tcatcgtccc
     6961 attctaaatc atggatatta aacattaatg tacgactcgc atttgaataa tcagtaatat
     7021 gcgcggcttt tcctgataat ttccatacta accaagtatc aatcgttcca aataatagat
     7081 cgccattttc tgctttttct cgtgcacctt caacattgtc tagaatccat ttaacttttg
     7141 tacctgcaaa atacggatct aaaagtaatc ctgtcttatc tctaaatgtt tgttcatatc
     7201 cttgttgttt taattctgaa caaattgatt gtgtttgacg tgattgccaa acaattgcgt
     7261 gataaattgg gcggccagta tgtttgtccc aaacaaccgt tgtttcacgt tggtttgtaa
     7321 taccgatacc tgcaatttga tcagctctaa catcattttc attaattact tccgtcatta
     7381 cagctaacac agatgtccaa atttcatttg catcatgttc aacccaacct gattgtggaa
     7441 aatattgctt aaactcacgt tgtgctaccc ctgcaatttc ccctttttga ttgaataaaa
     7501 tcgctcttga gcttgttgtt ccttggtcta tagataaaat atatttttcc attagtagta
     7561 tctcctttat taaaaaaata atcgctaacc cattgtacaa aatccgttaa attttggcga
     7621 aaaaatagca taatccatga atcaaacatg ttaaattatg ctatttacat ttctttttat
     7681 tttagtaaat tgattcgata tcaccttttt ttgatgattt atttaaaatg taacctaaaa
     7741 tcaaagtaat aataactaca acaattgcaa ttgcacaacc aatattaaat gtatgtttat
     7801 aaaatacagc gtatactacc gcacctaata aaccaccggc aattggtcct aagataggaa
     7861 cgattgcata tgaccaattt gaaccacctt tgccagctat tggtaaaatc gcatgtgcaa
     7921 ttctcggacc taaatcacgt gctgggttga ttgcataacc agtagcaccg cctaaactta
     7981 atccgattgc aacaattaat gctccgacaa ttaaaggatt taaaccatca gcaattttgt
     8041 ttacaccgat aaataaaata cctaaagtta atgccattgt tccgataatt tcacttaaaa
     8101 agttggcaaa gtaattctta atagccggtg ctgtagagaa aacacctaat ttcgcgccag
     8161 cttcttctgt cgctttccaa tgtggcaagt acattaacca tacaattgtt gctccgacaa
     8221 ttgcacctaa catttgagca acaatataac caggaactaa tgaccaatca aaacttccgt
     8281 ctaatgcaag agctaaagac accgctgggt ttaaatgtgc acctgagaat tgaccaacag
     8341 catacacacc cattgtaacc gctaatcccc atccagctgt gatgacaatc caatcagcac
     8401 cattcgcagc acttctcttt aaattgacat tggcacaaac gccaccacca aaaaggatta
     8461 agattgcagt tcctaggaat tctgctaaat atacattcat attaaatgtc ctcccaaaaa
     8521 gacaaagaga ttcctacact agcagtttgg ctcgtagaaa tctcccgtct ctattctatg
     8581 tattaacttt tattatattt gttataatac tattgagatg aaagcgttgt caatggtttt
     8641 tgtaaaaatt ttgtcaaatt taatttttca agtctttata aaagtgattt aatttgaaaa
     8701 ctagaaatat caatcctaac taaattattt atgacgccat tttgtttaaa tgacattctt
     8761 cagccatatt ttttatgact agttgcattt tttacaaata aacacctcta taactttaat
     8821 gattttaatc gttttagagg tgctaattat tttatttaat tattctttgt atgatacctt
     8881 aaattcctta ttacatatcc catatgaaaa cggctttttt aatatgttat atgactaaat
     8941 ctagtgaaaa atctaatttt tgcagactta tgattcacca gagtttatca taactagttg
     9001 ttacatattt cgctccattt ttaacagctt cattaacttc atctattgta ttaattaggc
     9061 cacctgcaat gacttgtgtg tttgtttctt tctgaatatg atgaatcgct ttactcgcaa
     9121 cacccggaag tacttcaaca aaatcaggtt caactttttt tatcaaatct atactgcgtt
     9181 tcaatgcttg actatcaata ataaacactc taaaaatcgt taaagtattt aatgatttcg
     9241 ctttttttat tactttagat ttagtcgata caataccttt tggcttgtat tgctgaataa
     9301 taaattcact tgcaaattca tcgtgactta aaccttttat caaatctata tgaataaaac
     9361 actctatatg attttgcttc agcaattcca taatactttt tatatgtcct atatgcatat
     9421 ctagaagcac acacatttta taatctgttt taatcagttt ctctaaatct ttaatgtttc
     9481 ttatggcagg caatatgttg ttattcactt gattcatcct ctctacatca cacgcttaaa
     9541 taatttttct aattcgtatt ttgaaaaatt aatgataatt ggacgaccat gtggacatgt
     9601 aaatggatct tccgcttctc ttaattgatc aattaaatca gacatttcat gtttttgtaa
     9661 ataatgattc gctttaatag attttttaca tgacatcatg attgctacat cttcacgtaa
     9721 ttttttgata tctacttttt tctcttccaa aattagctca atcatatctt taataatttc
     9781 ttctacttca tctttaggga accaaactgg atagctactt acaatataat catgaccacc
     9841 aaaatgttct aacatgatac ctacttgttg aagctcattt ttatattgat caatgactaa
     9901 ttgttcatct tttgaaaaat gaaatgttaa cgggattaat aaatcttgta tttcattggt
     9961 aacttcacct attttatctc gaaaatattc atattttatt ctttcttgag ctgcatgctg
    10021 gtcaatcatg tacatgccaa attcattttg agcaataata tacgttccat gtacttggcc
    10081 aacaatttcc atatatggaa ttcttcgttt agggtcttta ctttttgttc ctttaatatc
    10141 gtttacatca taataatcat cagacgcttt attctcataa ttttgcacag atgtatcgtc
    10201 atttgaatca aatgtttgat ccatttcttg taaaatttct tgttgttttt tgaagtagtc
    10261 ttcatctttt tcacgtgtat tattataacc agaagattca tcatcagcta ttaaactttc
    10321 agatgttttc gttacgaatg gattatatga atcttctttt ataactatct catcgttttg
    10381 attttctgcc ataaatggct tactgttact ttcttcagat gaaaacgtct tctcttgttt
    10441 attctctgtg ttttgtcttt gttcaaattc gattttttgt tgttcgaatg aatgtaacac
    10501 tttatttttt ttcggcacat aatccaagtt atttttagga attaatatac ggtctttaaa
    10561 tgcttcttgt attttgctca ctatcaattg atatagttgc tcttcttttg ataaacgcac
    10621 ttctagtttt gttggatgaa catttacgtc tactaagatt ggatccattt caatattaat
    10681 ataacaaatc gggaacctac ctattgttaa gagtgtatga tagccttcta aaatcgcttt
    10741 atttaacata aagtttttaa tgtatcgtcc attaataaaa atagaaatat agtgcttatt
    10801 acttctagaa tgttcaggct ttgcaacaaa accttcaatg tgataatcac ttgtatctcc
    10861 agatatatgt actaaatctc gtgcaacttt catcccataa atctctgcca tcacttcatt
    10921 agttcttcct gaaccatttg tacttaacat tgttttgcca tctgaaatga gtgctattcg
    10981 aatgtccgga tgactcattg ccattctgtt gacaatatct gttattttac ctagttcagt
    11041 gtataaactt tttatatatt ttaaacgtgc tggtgtatta taaaataatg attctacaag
    11101 tatatctgtt ccttttttcg cttttgcagg cttatgattt aatatttcac cattttctac
    11161 atatatttca tttccattag cattatccgt gcaagtcttc aatgttactt tagcaactga
    11221 tgaaatactg gctagtgctt caccacggaa tcctaacgtc ctaatatgaa ataaatcttc
    11281 atcttgatct aacttactag tcgcatgtct atgaaatact aatcctaagt cttccgcttc
    11341 aattccgctt ccattatcga ctacgcgaat agattggacg ccagattcct ctacttcaat
    11401 gcttatttct gtagcgcctg catctatagc attttccaac agttctttca caacagaact
    11461 cggtctttca actacttcac ctgctgcgat tttatttgct aatgaggttt ggagttcttt
    11521 aattttcccc attttgcaac acctctattt taattgattt tgtaattcac ttaacttcac
    11581 caatgcctca attggtgtca tattagataa attcaaattt ttaatttgta gttcaatctc
    11641 gctttcttga tcattttcaa acaaatcaaa tgatgcttgt tcaaagtctt tttgagataa
    11701 agtatcagtt gtttcttcaa cacttaagtt tgaattttct tgattaattt caggttcatt
    11761 ttcgaccatt tttaaatttg atatcgatga ttttttacca gcagacgctt caaactcgct
    11821 taggatcact tgtgctctgc taataacttt ttcaggtaaa tcagctaatt tcgcaacttg
    11881 aataccataa ctatcgtcaa ctgcaccatc tttgacttta tgcaagaata taagttcacc
    11941 tttatattca ttagcagcga cgtgaacatt ttttagactt ggtaatgctt gatctaatgt
    12001 tgtcaattca tgataatgtg ttgaaaataa cgttttagca tgtgatgttt cagctacata
    12061 ctctatcatt gcctgcgcta aagctaaacc gtcatacgtt gaagtaccac gtccaatttc
    12121 atcgaaaata atcaaactat cctctgttgc ataagttaat gccttttgtg cttctagcat
    12181 ttctaccata aacgtactct tacctgaaac caaatcatct gccgcaccta ttctagtgaa
    12241 tatttgatca aatataggta acacagcctc tttacaaggg acataagctc ccatttgggc
    12301 cattatacta attatggcaa cttgtctcat atacgtcgat ttaccagaca tattcggacc
    12361 tgtaattaaa tatataaatg tttcgttatc taatcgacaa ttattaggca catagtcatt
    12421 ataatccatt actctttcca ctactgggtg cctagattcc actaattcta atgttttatt
    12481 ttcactaaat gaaggcctag tataattata tttttgagca atttctgcaa agctctgtaa
    12541 acaatctaac tctgaaataa ttttagcttg atgttgtaaa cgttcagtat attttttaac
    12601 ttcttcacgt agctgaacaa ataattgata ttctaattca atggctttgt cttccgcacc
    12661 taaaatgata tcttcttttt ctttaagttc atcagttata aatcgttccg cattcgataa
    12721 cgtttgcttc ctcatataac caaattcact tggttcaaaa ttttgcaagt tggcacgtgt
    12781 tatttctata aaataaccaa acactttatt aaagcttatt ttcaatgatt ttattcctgt
    12841 acgttgtctt tcttttgctt gtagttctgc taaccatgtt tttccgtttt ttgaagcttc
    12901 aagatattca tctaattgca tattaaaacc aactttgaat agtccaccat ctttaactga
    12961 aattggtggt tcttctacta aactctgttc taatatatca agtaaatcat caagtggttc
    13021 tagttgatta acttgtacaa gagtatcctg attcatagaa tttagtaatg ctttaatatt
    13081 cggtatttca gaaatggaat gtttaagttg aattaaatct ctcgcattaa catttccgta
    13141 actaacacgc ccaacaagac gttcaatatc atacacttga ttaagatatg ttcttaaggt
    13201 gtctctttct atgaaatgag cactaaattc atcaacgata tctaatcgtg cttcaatttg
    13261 ttctttactt attagtggtc tatctatcca ttgttttaat cggcgtgctc ccattggtgt
    13321 tttcgtttcg tccatcagcc aaagtagcgt tccttttttg gattttaatc gaatgctttc
    13381 cgttaactca aggtttctct tagcataaaa atccattttc atataatcta tagctgcata
    13441 ttgaacagca tcctcaatat gcgataaatc acgtttttgt gtatgatgaa tataatctag
    13501 caataattgt gtcgcttgat acattaattt atgttcagtt tgattcacac tgtagatttc
    13561 agatgataac gtttccctga ctgttattgt ttctgtcacc atattaattt gtctttttaa
    13621 atgatcggaa atattgtcat tgataacaac ttcgttaggg tttatcgtcg taatttcatt
    13681 taataaagtc gcttcatcat taaaatgtgt aacctttaat tcaccagtag aaacatcaca
    13741 gtaactaagc gcaatttcag gttgattcat aacaaaactt aaaatatagt tattttgttt
    13801 atcatctaca ccaccttgct ccatcacagt tcctggagtc acaattctta ctacctcacg
    13861 tctaaccata cctttcgttt gtttcggatc ttccatctgt tcacaaatag ctactttata
    13921 tccattatta acaagtgtat ctatataact atctgcagaa tgatacggaa caccacacat
    13981 cggaattgga ttttcttttt tagcatctct tttagttaaa gtaatttcaa gtacacgtga
    14041 tgcctccttg gcatcttcat aaaacatttc atagaaatca cctagtctaa aaaataataa
    14101 gcaatcttgg tattctgatt ttatttttaa atattgctgc atcattggtg taacattaga
    14161 catattattt cttcacaacc cttgtctctt tttaaaattt gtctttacaa tatattcgtt
    14221 tgtaagcttt ctaattatta attatttaac ttatacattt taacatactt acttttacaa
    14281 acctattcat accatataat aacgaagcat cttaaatgta taagaaaacg cctcaaacct
    14341 aataaaatgt gttaatagca tgtttagaat tatattaaaa ttctaacatt caagacattt
    14401 aattaagtaa gggcgttcaa tattaaaatg aacaatgact ctgtttgaaa tcatatatca
    14461 taaaattatt ttataaacct ttgaagaata ccacgttttt ttagagtaag taataagaaa
    14521 taacttataa tagatccgat agcacttgag actatgaacg taatcattaa cggtttaatg
    14581 aagaagtctt gaagcccaag gaaatatgct aatggtatac aaattaaact tccgatgaca
    14641 ccagttccaa gtacttcacc gaccgcggcc atgaatatat gtttacgata tatgtaaaac
    14701 atactagcca ataaaactcc aatcatacta cccggaaatg caaaagctgt accagttcca
    14761 aaaagaactc ttaaaattga tgatataagc gcttgagcta atccatacca aggacctact
    14821 atgaccgcac ttaatacatt tacaaaatgc tgtactggtg ctgccttaac tggtcctaga
    14881 ggaatgatga taatactgct taatacaaca tttattgcaa ttaaaagtgc agttatagcc
    14941 agttttcttg atttcatatg attgttctcc tttttgtttg taattaatca ctatgtttgg
    15001 ctttattatg gtcatttaaa cgtgtttcca ttgttgatac aaacattttc aataattgat
    15061 tcgcttcata ttgtgaagtt tgaaactgtt caactatggg caatgtattt atttctgctt
    15121 ctatactttg aatggtatgt tccgactgtt ctagcgcatt ttgtttcccg taattttgaa
    15181 agtttactgc ttgtttttga tgctttttta acatatccat tttagtcttt atcgtttggt
    15241 tctgatgaat ctgtgcttca atttgttgat atgttttgat agtatccaaa tttttaattt
    15301 tatttgcaat attatccgct tgtttcaaca cgtcatcttt attatacatt tattgaatca
    15361 ccatttccgg ctctacttcc tttacaaagc tgccatttaa tgaatactgt ttagcttcat
    15421 ctattcgtac ttccactagt ttaccaatca tttctttagg cgctttgaaa ttaactaact
    15481 tatttttatc agtgtagcca gcaagaacct gatcatcttt tttactacta ccttcacaaa
    15541 gtactgttac agtttgtcct tcgtacttac tcatagctat ttgtgaataa tgaccaactt
    15601 ttttattcaa acgttgcaat cgttcctttt tgacatctaa aggtacatta tctttcattt
    15661 tagcagcagg cgtaccatca cgttgtgaat acaaatacgt atatgcatgt tcaaaaccaa
    15721 cttcatcata caaagttaaa gtttcttcaa attgttcctc tgattcattt ggatacccta
    15781 caataatatc tgtagttaat gctacatttg gaattctatc tttgattcgt tttactaaat
    15841 ccaaataact ttctcgtgta tattttctac ccattatttt taatactgca ttatttccag
    15901 attgaactgg caagtggata tgaggaacga tattaccacc ctctgaaata acatcaatca
    15961 tgtgatctgt gaagtcccaa ggatgacttg ttgtgaaacg aactcttgga atcgctattt
    16021 tagaaattgc ttgtaaaagg tctcctaagt cacattctat atcctgtaaa tctttaccat
    16081 aagaatttac attttgacct aaaagcgtta tttctttgta accttcacga gcaagttcac
    16141 gtacttcatc tataatgtct tcaggtctac ggcttcgttc tttgcctctt gtaaatggaa
    16201 caatacaata tgtacaaaac ttatcacaac catacataat attgacccat gctttaatgt
    16261 tgccttcacg gacttttgga agattttcaa taacgtctcc ttctttagac catacttcaa
    16321 caaccattgc tttagataag tatgcttctt ctaaaatttc tggtaaatga tgaatattgt
    16381 gtgtaccaaa tatcatatct acattttgat acgattttaa aattttattc actactgact
    16441 cttcttgtga catacaacca caaactccga ttaaaatatc aggtcgttct ttcttcaaat
    16501 gcttcaaatt acctatttca ctaaaaactt tgttctcggc attttctcta atcgcacatg
    16561 tattaattaa aataacatct gcagtgttaa tatcagtcgt tgcttgatag cctaatgctt
    16621 caagtatacc agcaatgacc tcagtgtcat gtgcattcat ttgacatcca tatgttttaa
    16681 ttaaaaatgt acgctcgttc cccataccgc gatatttttc atcaatttgg aaatctctat
    16741 tataacgaac ttcttgttta cctctttttt tcgcttcttt taaattaggc ggttgataaa
    16801 catgttcaaa atatttacta taatcttttt ctgctttctt atctctctca gctaaaacat
    16861 ctacagaact tgcttttctt tgttcttcgt tcactttaca aaatcctttc tatttgcccg
    16921 tatcatttaa taattatatt aaatttaact ataaagtgca aaaacagtta aagttatacg
    16981 tcatctctaa caagccaatt caaatccagt ttacttcact tttaatgtta ttcatcatat
    17041 ttttcagtta aagtttcaat agtaatgccg tctggcacat gataaaactt aacttggcta
    17101 ataatacttg gacattcaag ttccaccatt cgttcattta acgattgtat taaaattaaa
    17161 cattcattca tatttccttg tactgtcgtt tctaacggac ctactctaaa atgcaaacca
    17221 gattcgtcaa ttattttaat cgcctcgtct acgtaaggta taacattgtc attgtctggt
    17281 gttttaggaa ttatttgtac actcattaat gtgtctttca tttttaaaaa ctcctttata
    17341 ttttgtaata gtaaaaaaac tgagtagcat aaatggatct gttataaata catttatacg
    17401 actcagtttt aattattaaa taaattgttc agttaatgca ttgaacgtgt cttcagtaat
    17461 tttgatatct cttttagcta gtggtgtatc atctaactct ttgatttgag attcatatga
    17521 tggggtttct ttatcttgat aaacaatacc agttactaaa gattcatgtt caataacagt
    17581 tttagtcgct aattgtttat ctgtagaatc atagttttca atgtcatcaa cacttgttaa
    17641 atgttcttta aaccaatcgt atgtgttaat tttattataa gtcacacatg gtgaaaagac
    17701 attaacaaat gaaaacccat cgtgattaat tgcatcttca attagttttg ttaatccttt
    17761 aatatcactt gaaaaacctt gggctacaaa tgtggcacca gatgataatg ctaattctaa
    17821 aggcgcaaca tttttttcta tattaccttt tggcgttgtt ttagtaacaa atcctactgc
    17881 tgatgacggc gatgtttgtc cctttgtcaa accataaatt tgattatcca tgactatata
    17941 cgtcatgttc atatttcttc ttaaagcatg gattgtatgc cccataccaa tagcataacc
    18001 atcaccatct cctccagatg caataacagt taaatcttta ttcgccattt ttacaccttg
    18061 agctaaaggt aatgcacgtc cgtgaataga atgaacgcca taagaattaa tatatcctga
    18121 aagacggcca gaacatccta taccggtgat gatagctact tgttcaggtt ctaaccctat
    18181 atttgcggct gctttttgaa ttgcagcttg tactgagaaa tcgccacatc cagggcacca
    18241 gttaggctta acattatttc taaaatcttt aaatgtcggc atctatacca tctcctttat
    18301 ttcagtagca attattttac ctttttcttc gatttcatgt ggtaggaaag gtgtcccatc
    18361 atactttgta taattttcaa tcttatcatg aatattgaca ttcattttta taatactagc
    18421 caattgtcct tgataattgt gctccactac aacgactttc ttcgctttat taactgcatc
    18481 ttgaataacg cttgttggga atggatgcaa ttgtctaatt tgtatagtgt taacttttat
    18541 gccttgttga ttcaaacgat tactaccttc ttgaattgca ccttttgtag aaataaaacc
    18601 gatataaaga atatctgcat cttcatgttg taagttagct tctactggcg attcaattag
    18661 taactgctca attttacgca ttcgtttttc catttgttgt tgtctatttg acgcagattc
    18721 gctaggttta ccttcttcat tgtgttccac accagttata tgatgaatac ctcctttaac
    18781 acccgggata ggtctaggag aaacaccatt ggatgttaac gcataacgct tgaaataacc
    18841 tttatcatct tcttcacgtt caatatcaga ttgaatgatt tcaccacgtt taatttcaat
    18901 gcgattataa tctaattttt caacagtttg ttttcctaaa gataattgta aatcacttag
    18961 aactataaca gggcattgat attgttctgc taaattaaag gcttccatag ttaaataaaa
    19021 tgcatcttct gcatcagtag gtgctacaac tatttttgga atatcaccat gtgtaccata
    19081 aatcatttgc attaaatctg actgttcttg tttcgtaggt aatccagtag aaggtccacc
    19141 tcgttgggta ttaattatga ctaatggcgt ttcagtcata ccagataatc caattgcttc
    19201 catcattaaa gataaacctg gaccagcact agccgtaaac gctctgactc caccataatt
    19261 tgcaccaata gccatagtta cagcagcaat ttcatcttct gtttgaataa ccgctccgtt
    19321 tactttagat atattggcaa tcatatattc cataacttca gacgcaggtg taataggata
    19381 tgccgccata aattgtgaac ctgcagcaat tgcacctaat ccaatggcat cgttaccaat
    19441 catatataga tgtggtagtg catctgttga ctctaattca aagtccccgt cgatttcaga
    19501 taagcgagat tgcattaatt gataaccttc gtttaatgct tggatattga cttcaactac
    19561 cttgtcacct tttttagaaa acatattagt aataagttct tcaaatgtat ttgtattcaa
    19621 attcatcaat gcgctagtag cacctattgc aaccatgttt ttcattaatg ctgtacctaa
    19681 ttctttagcg gttgctgtaa aaggtaattc aataagctgt gcatgacatc cttctggttt
    19741 cacaggttta gccttggcat cagctaaaat gatactgtct tctctcattt catgatggtt
    19801 aacatcaatt gtttcttggt caaatgcaat caaaatatct aaatcatcac taattgcatg
    19861 aacaggcgtc gtagaaactc taattttatt attcgtatgt ccacctttga tacgacttga
    19921 aaaatgtcta tatccatata aataatatcc ttttctattc atagccgttg cgaagatttc
    19981 cccagttgat tcaatacctt cgccttgttg accgccaact ttccacgata attgtggttt
    20041 catagttata tgcctcctat ggatatattt catattaaat gataacaaaa agaaccaccg
    20101 atttactatg taaacagtgg tactatatta aattcatact aatggacgat agggtccaat
    20161 tagaatgttt acttacgtaa ttttaaaatg ttgaaaatgg atggtcatca tttatcaata
    20221 tacgttcgat gtgctttgtt ttaccttctt tgtccaaatc aataacaaca ccagataata
    20281 cacttctacc ttcatttgga acaacatgtc tttgtggcaa actagtgata aaacgttcaa
    20341 ttacctctgt tttatttatt cctaaaatgc cgtcataaaa gcctgtcata ccaacatccg
    20401 ttatataccc tgtacccttt ggtaaaatac gttcatctgc tgtttgaatg tgtgtatgcg
    20461 ttccaacaac agcgctagct ctaccatcta aatgccatcc cattgcatac ttttcagaag
    20521 ttgtttctgc atgaaaatca acaaatataa acggagtttg ttcttgtgct tccttgacta
    20581 attgatctgc ctttttaaaa ggatcatcaa tatctggcat aaacgctctt ccttgcagat
    20641 taataactgc aagtttaata tcattaattt ttataaatct cataccaatt cccggcgctt
    20701 catccggaaa attagctggt cttactagtc gttttgcttc atctataaaa tcataaattt
    20761 cacgttgacc atatgtgtga ttacccatag tcatgaaatc tacaccattt cttagtaatt
    20821 gtttatatat tttttcagtc aaacctttac catgcgctgc attttcagca tttacaattg
    20881 taactgttgg tttatacttt tgcttcagtt gaggtatgta cgtttcaatt gcgtctcgtc
    20941 caattttacc tacgatatcc cctataaaca ttattctcat gttttgttca tcctctcata
    21001 aatactagtt taattcatac gttctaatat ttaaagtaaa tattaaattc tttgaattat
    21061 ttttcttcaa ttatagagag acattacttt gtctgaaaac ttcaatatgt tagtattaag
    21121 acatcaaaat aaataggagt tgtaaataaa tacatgtcta ttaatattga tccagaaaaa
    21181 tttgcagaat tagtggttat gtctaaccca tcaa
//
LOCUS       DATTQF010000030        20007 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.030, whole genome shotgun sequence.
ACCESSION   DATTQF010000030 DATTQF010000000
VERSION     DATTQF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.030"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            112..480
                     /locus_tag="VB241_001009"
     CDS             112..480
                     /locus_tag="VB241_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEP1244313.1"
                     /translation="MKTILKTITYLAFTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            661..1233
                     /locus_tag="VB241_001010"
     CDS             661..1233
                     /locus_tag="VB241_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP1244314.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVDD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDQ"
     gene            complement(1371..1634)
                     /locus_tag="VB241_001011"
     CDS             complement(1371..1634)
                     /locus_tag="VB241_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1244315.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            1928..2182
                     /locus_tag="VB241_001012"
     CDS             1928..2182
                     /locus_tag="VB241_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP1244316.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIIFIAIVTLILRAVRKK"
     gene            complement(2221..2430)
                     /locus_tag="VB241_001013"
     CDS             complement(2221..2430)
                     /locus_tag="VB241_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244317.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILVFLFPTSSHVNLLLF
                     NPILPTFSNIIIHIDHFHANKKDDN"
     gene            2608..3189
                     /locus_tag="VB241_001014"
     CDS             2608..3189
                     /locus_tag="VB241_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1244318.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(3255..3638)
                     /locus_tag="VB241_001015"
     CDS             complement(3255..3638)
                     /locus_tag="VB241_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244319.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPALILAIFNNMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSFDKNDSH"
     gene            complement(3909..4535)
                     /locus_tag="VB241_001016"
     CDS             complement(3909..4535)
                     /locus_tag="VB241_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HEP1244320.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVNEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(4608..6227)
                     /locus_tag="VB241_001017"
     CDS             complement(4608..6227)
                     /locus_tag="VB241_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244321.1"
                     /translation="MLDVHFIYPGKLQNACEYFNLPTQKEGFEVTIHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQINEDEFFVNNLFLSY
                     INSEVLDSTYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNTTLFEFDILSSILESDANYRL
                     NNELIEFEKLQLINFDNFLTSHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVDEQTIENSLAEFENALELSFMEDEVDLDMLLEHDDFEDFGTTDSLRSDAITQ
                     LISKIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSLD
                     TLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLKS
                     DQAIKEFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLVQPIMQLFIDNAILNN
                     VQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            complement(6342..7865)
                     /gene="ahpF"
                     /locus_tag="VB241_001018"
     CDS             complement(6342..7865)
                     /gene="ahpF"
                     /locus_tag="VB241_001018"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEP1244322.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGENHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(7881..8450)
                     /gene="ahpC"
                     /locus_tag="VB241_001019"
     CDS             complement(7881..8450)
                     /gene="ahpC"
                     /locus_tag="VB241_001019"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP1244323.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            8941..9696
                     /locus_tag="VB241_001020"
     CDS             8941..9696
                     /locus_tag="VB241_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEP1244324.1"
                     /translation="MSDHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEQIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVARVREILDLPDYVFPLFG
                     MAVGEPAEDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISKYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(9776..11164)
                     /locus_tag="VB241_001021"
     CDS             complement(9776..11164)
                     /locus_tag="VB241_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEP1244325.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALMFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYIKKTLEVLIFAFTSRSSAGTLPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            11249..11350
                     /locus_tag="VB241_001022"
     CDS             11249..11350
                     /locus_tag="VB241_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244326.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            complement(12283..13239)
                     /locus_tag="VB241_001023"
     CDS             complement(12283..13239)
                     /locus_tag="VB241_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244327.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTTKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESSNKVENNTATDEKAESKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQPKNDKAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(13357..14019)
                     /locus_tag="VB241_001024"
     CDS             complement(13357..14019)
                     /locus_tag="VB241_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244328.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGQTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYDLLEEMDLRK
                     PF"
     gene            complement(14162..14569)
                     /locus_tag="VB241_001025"
     CDS             complement(14162..14569)
                     /locus_tag="VB241_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEP1244329.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDKMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVGV
                     RDHSSHQEVLENNSAYEEVDITHFAEASKGPKA"
     regulatory      14749..14851
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15082..15660
                     /gene="xpt"
                     /locus_tag="VB241_001026"
     CDS             15082..15660
                     /gene="xpt"
                     /locus_tag="VB241_001026"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP1244330.1"
                     /translation="MELLGQKVKEDGVVIDERILKVDGFLNHQIDAKLMDEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     gene            15660..16928
                     /locus_tag="VB241_001027"
     CDS             15660..16928
                     /locus_tag="VB241_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP1244331.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFKFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            16966..18432
                     /gene="guaB"
                     /locus_tag="VB241_001028"
     CDS             16966..18432
                     /gene="guaB"
                     /locus_tag="VB241_001028"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP1244332.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            18457..19998
                     /gene="guaA"
                     /locus_tag="VB241_001029"
     CDS             18457..19998
                     /gene="guaA"
                     /locus_tag="VB241_001029"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP1244333.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
BASE COUNT         6643 a         3101 c         3670 g         6593 t
ORIGIN      
        1 tatctttcag attcaaatac catcattttc tcctaagact tacactttaa ttacaattat
       61 gtaagttgtt ttcagagaca tcctatttta tattttgtca gaggtgatct tttgaaaacg
      121 attttaaaaa caataacata tcttgcattt actatcattg gcgcttatgc tgctttattc
      181 attttaaaaa caatagactc tcatggtata acagatcaat ttaacccatt agtaaaggaa
      241 gatgattctt atgttaaaac gacagaggtg tctactagaa tggatgatca actccgaagt
      301 tatagtcaaa gtgcttttaa taaagaaggg aaagagacgc aattaatgta tactgctaca
      361 tttgatgtta aaccgcatag atacttgaaa attacacata aaggtcatca tgtagaaact
      421 tttgaagaag tagaaaagga agaagtacca aagaaagcat tagacaaact gagtcgataa
      481 tagcattctt atattacatg gttcatttta aagatgagta cgaaatgaag tgacacatga
      541 cgattaattt aaaaatattt gtaataatta tgaatagaat taaaaacaag gggtaataca
      601 atctatatag catataagct tttgttatga gtttcaaaaa aaggaagaga gagtgatatt
      661 atgaaattaa aatcattagc agtgttatca atgtcagcgg tggtgcttac tgcatgtggc
      721 aatgatactc caaaagatga aacaaaatca acagagtcaa atactaatca agacactaat
      781 acaacaaaag atgttattgc attaaaagat gttaaaacaa gcccagaaga tgctgtgaaa
      841 aaagctgaag aaacttacaa aggccaaaag ttgaaaggaa tttcatttga aaattctaat
      901 ggtgaatggg cttataaagt gacacaacaa aaatctggtg aagagtcaga agtacttgtt
      961 gatgataaaa ataaaaaagt gattaacaaa aagactgaaa aagaagatac agtgaatgaa
     1021 aatgataact ttaaatatag cgatgctata gattacaaaa aagccattaa agaaggacaa
     1081 aaggaatttg atggtgatat taaagaatgg tcacttgaaa aagatgatgg taaacttgtt
     1141 tacaatatcg atttgaaaaa aggtaataaa aaacaagaag ttactgttga tgctaagaac
     1201 ggtaaagtat taaagagtga gcaagatcaa taaaatatgc cgtaacacta acgatggcaa
     1261 catgtcttaa ttcatattaa ttattaaaat accctcacaa cgcaatgtaa tatatcccgt
     1321 atagatttga aaggatatat gcctaacgtg ttgtgagggt ttaattgcat ctatttatcc
     1381 atgcgaatat cgacttcttc taaatgtttc tgatattctt taaccttact ttctaaaaac
     1441 atttcatatg gtgcatcaaa gaagtcagct aaatgcatgg catctttaaa tttaggttca
     1501 tggtgatgat tctcccattc ccaaatttga tgtgcttcat atttagtacc atatttctca
     1561 tttaattgtt gtgctaattc gtcaatttct aaattatgtt tagttcgtaa gttatataaa
     1621 atatgcatat tcattgttca ttaacctcgc ttcttgattt aaaattcaaa gatgctctta
     1681 taatatgtgt atattctagt cctataacat aaaataatca agctatgaat tcggcagaaa
     1741 aaataatgta tatgatattg ccggtacaga gactttaaat aacgatagct acattgaata
     1801 aaattgatat tcaattacta cttttaaaaa tatttggata aaaataattt gaatttgttt
     1861 tagaaagtct aataatgggt actacttaga ataacgcaaa ataaatagaa aaaggagact
     1921 gaaaattatg tttggattta ttggaatgtt aattgtcggt ggcttaattg gatgggctgc
     1981 tggtgctatt atgggtaaag atatcccagg tggtatttta ggtaatatta tcgcaggtat
     2041 tattggatca tgggtaggtg gcaaactatt cggacaatgg ggtcctgaat taggaagtat
     2101 ttacatcttg ccagctttaa tcggttcaat tatctttatt gcaatcgtaa cgttaatttt
     2161 aagagcggtt cgtaaaaaat aaaatattca acttaggttg aattaatata aagatggcat
     2221 tcagttgtca tcttttttat ttgcgtgaaa atggtcaata tgtattataa tatttgaaaa
     2281 tgtaggtaag ataggattaa aaagtaataa attaacatga gaagaggttg gaaataaaaa
     2341 aacgagtatt tgacaaataa aaaaaggatg acgttgggtt gcgccatcca ggagtattat
     2401 gttcatgaat ataaaagact gtgtagtcat ttaagaacta cacccatata gtaacacatc
     2461 acttgattaa agacaatact atttttagag tcattaattt aaaatatgaa ttaattcttt
     2521 ttttagaata gaatattaag attgatatcg aattagtagt caaagtgtta tggtagatat
     2581 gaaatacata agataaggag taatattatg acgatttatt tagttagaca tggcgaatca
     2641 aaatcgaatt atgataataa acattttaga tcttattttt gtggacaatt agatgtgccg
     2701 ttaacggata ctggcacaaa aagtgcggac gatttatgtg attattttaa agagaaacag
     2761 attaaacatg tatatgtttc agacttatta agaacacagc aaacgtttga acatattttt
     2821 ccatatgaca ttgcatcaac gactacccct ctattaagag aacgttcact tggcgtattt
     2881 gagggtgaat ataaagatga aatcagtgcg aatccgaaat atgaaaaata tttcaatgat
     2941 ccaaacttta aagactttcg acatagtttt tcacaaaaag cgcctgaagg agaaagttat
     3001 gaagatgtat atcaacgcgt agaacatttt atgaatcatg ttgtcaatga agatacacaa
     3061 aaagatgata tcgtcattgt tgcacatcaa gttgtcattc ggtgtttgat ggtttatttt
     3121 aacaaagttt caagggaaga agctgtggat ttaaaggtag aaaattgcaa accatatatc
     3181 attgaataga agtatagagg ttctgaaaag ctgtgtttta tagcggcctt cagaaccttt
     3241 tttgtatttg gattttaatg ggaatcattc ttatcaaaag acttcccacc aatagctaat
     3301 gtgataattg cgcttatgaa caaaataatt gaagttataa atgacgctaa tgtaaaaggt
     3361 gtaaattgtg tgagcgataa tatcaatgat aaaaagaacc aagagatagc ccctagaaat
     3421 tgtaaaatag cagaagaaat tctaaaactc atattattga atatagctaa aataagtgcg
     3481 ggtaatgcga ctaataccat tataatgatg ttgagtgtga ataaatatgg ctgttcgaaa
     3541 gttactgtgt ttggacctgt tggatgcatt gctgctaaga acaagcaaac aatcgtcgat
     3601 aaaattgcta aaataataaa agtgattcga actttcatca tgaccatcct ttgtttatag
     3661 agtcaatata agtatggaat atgttaggga tatagtcaaa tgcgtcaact aatggggatt
     3721 ttaggatgga tagagaattt aaggcaatta aaaaagcatc aaacagtaat atgctgcttg
     3781 atgcaatatg gtattggtta aaatgtattg tctcaaagat tagtcacttt taaagataaa
     3841 gaattgtcat gaattaaagc gcatgtactg atgagtgatg tgttacatgt cgcaatgatg
     3901 gctttcagtt atttatcgat aacatcactc ttgatacctt tagattttaa gaaatcttta
     3961 attttatctt gttgcttttt attaacatca ccggcatatt ttgttggcac gtcgactaca
     4021 ttgattttat tttgcggttg atagctaagc ttttcaatat cttcgttaac attggcgatt
     4081 gtactattta aagctttgaa gtaattcatc attaattcaa cgggtttctt atattcttta
     4141 ggaatattgt tttcagtgac aaatttcttg aaatgcaaat catttttaac agctaagtta
     4201 gataagtggc taagtgtttc tgcttgtttt tcagtcactt ttgtttgact gtcaatttgt
     4261 ttatctagtt tatgttgcat aatatatttg ttatcaagta tatcgctatt tacagacaaa
     4321 tacttttcta tagcttgctt catctctgca tcactaatat cactattttt cttatctgag
     4381 ttaaaaatat cttttgtttc taatttttta gcgcttttag gtgcatggat gccagtactt
     4441 gtgtgatgat cttcgttatc agattgatcg gacgcgcaac ctgtaagaat taatgtcgat
     4501 gctaaaaatg tacttagtag taatctcttt ttcataatgt aatataactc cttagtttat
     4561 ctttaattga aaaaatatgt attcatgttt aatagagtaa cattgaatta gtttggaatg
     4621 tcacgatgac cttgcaatga ccatagacgt aaatgattac gtgcatgagt tgctttttct
     4681 atcaataatg cgtcgttttg aacgttgtta aggatagcat tatctataaa taactgcata
     4741 attggttgta ctaatttaga cgtaggtatt gtacgtaaaa gcataatgat ttcattcaca
     4801 tacttttcct tctcaatatc atttttcata ttgatttgtt tgcgtagata actggtaaac
     4861 tctttaattg cttgatcaga tttcaaatag tggtgtctat cataatagat taattcctca
     4921 acagaagctg gtacatagta tccttcatca gaaatatcaa tgcctgacgc ctcagactcg
     4981 acaacatcaa ataacacagg gtgaaacacg aagtcatctt caatataata tagctcagta
     5041 ccagctaaag tatctaagct atattgcaac tcttctttac taaaagtagc gtcataaaat
     5101 ctttgtgtga gatatagtag atgattagtt gataaaatac catacaagtt tacagaacca
     5161 agtataatac gaagaggtaa aatatctttt ttatctatta atcctttctt aatcttagaa
     5221 atcaattgag tgattgcatc acttcttaag ctatcggttg taccaaaatc ttcaaaatcg
     5281 tcatgttcaa gtaacatatc taagtcaact tcgtcttcca taaatgataa ttctaacgcg
     5341 ttttcaaact ctgctaaact attctcaatt gtttgctcat caacaaattg atttacagct
     5401 ttctcaaaat caccattgcc gacttgattc acgaaatttt caatatgttt acgcacatca
     5461 tttggcaaaa taagacaatc atattcaaag tcgtaataat aataaaaatg actagttaaa
     5521 aagttgtcaa aatttattaa ttgtagcttt tcaaattcaa ttagttcatt gtttaatcgg
     5581 tagttagcat cactttcaag tatgctagaa agaatgtcaa actcaaatag agtagtatta
     5641 gaaaacactt gttcaatgat gtgattatta ttgaaaaatg ctgtcataat taattcaaca
     5701 agattttgtt tgtttttatt tgagaatcct ttgataccat actgcctgca tattgttttt
     5761 agctcgtcat tagtttttaa cagtaaagca tgttctaatg tgacatcgtt taagcgcgta
     5821 tgttgtctta cacgtaacac ttcttcattt aaattcatat ggtttattgt atctaacgat
     5881 tcgaattcac cataggtact atctagtact tctgaattga tataacttaa aaataaatta
     5941 tttacaaaga actcatcttc attaatttga taaacaaaac ctagatgatt tgttagcttt
     6001 aaaatattaa catcgcgttt atttataatt gtgacaggat taccattaga tataagtgtt
     6061 tctataaatt tttgttctgt cttatccata tcatgataaa ttttatgtat attttcaggg
     6121 cgtaaagcgt gaatcgtgac ttcaaatcct tctttttggg tcggtaaatt aaaatattcg
     6181 catgcatttt gtaattttcc tgggtatata aaatgaacat caagcatttt tgcacacctc
     6241 gttgtatata ttaagtttat cataacatga ttttatgtcg ggataaaaaa ataacagcat
     6301 cttaacaaat gtaagatact gtcagtgaaa tgaatgaaac tttagtttct gataatatag
     6361 tcaaaggcat ttaatgctgc atttgcacca gcgcccattg aaataataat ttgtttgttc
     6421 ttctgatctg tgacatcgcc agcagcaaat attccaggta cgttagtatt attgttacga
     6481 tcaataacaa tttcaccacg ttcgtttaat tcaacagcat cttttaacca tgatgtgttt
     6541 ggaagtaaac caatttgaac aaagatacca tctaagttaa gtagatgttc ttcgccggtg
     6601 ctcatgtctt cgtaacgtat acctgtaaca tggttttctc cgacaacttc agtagttttg
     6661 gcatttgttt tgatatcaac atttgataaa gaacgtaaac gatcttgtaa cacgttgtct
     6721 gcttttaatt cgctagcgaa ttcgaataat gtaacatgat taacgatacc agcaaggtca
     6781 attgctgctt caaccccaga gttaccgcca ccgataactg ctacgtcttt attttcaaat
     6841 agaggtccgt cacagtgagg gcagaatgca acacctttat taatcaattg ctcttcacct
     6901 ggaatgttta gcttacgcca acctgcacca gtagcaataa tgactgtttt actttctagg
     6961 acagcaccgt tttctaacgt aactttaatt gcttcgtcag tcttttcgat atctgtagca
     7021 cgaatgcctg tcattgcatc aatgtcatat tgatcaatgt gcgctgctaa gttagaagaa
     7081 aattcagaac cagttgtttc tttaacagta atgaagttct caataccagc agtatcatta
     7141 acttggcccc cgatacgatc agcaactata ccagtacgta aacctttacg tgctgtgtaa
     7201 atcgctgcac taccactagc aggaccacca ccaacgatta agacatcata aggttctttg
     7261 ttttcaaact cagatgcatc tgccgtactg cctagtttcg aaagaatatc ttggattgtc
     7321 atacgaccat tgccaaattc ttcgccattt aaaaagacag cagggactgc catgatgttt
     7381 tcagattctt cacggaacac tgcaccatca atcatagaat gcgtgatgtt agggttaatc
     7441 acactcatta agttaagtgc ttgaacgaca tcaggacatt tttgacacgt taaactaatg
     7501 aatgtttcaa aatggaatga accttctaag tttttaattt ggtcaatgat tgactgtttt
     7561 tctttaggtg cacgaccact aacctgtaaa attgctaaaa caagtgagtt aaactcgtga
     7621 cctaatggaa tacctgcaaa tgttacacct gtttcttcgc caggacgatt gactgagaaa
     7681 cttggtgtac gttttaaaga cttttcagaa agtgaaagtc taggtgacat atcagaaatt
     7741 tctgttaata attctttgag ttccttagat ttatcatctg aaccaaggct ggcaacgaat
     7801 tcaacgttgc cctccattag ttctaatagt tgtttaagtt gttgttttaa atcagcatta
     7861 agcatggttg taatgcctcc ttagatttta cctactaaat ctaaaccagg ttgcaatgtt
     7921 ttagcgcctt cttcccattt agctgggcat acttcgccag ggtttttgcg aacatattga
     7981 gctgctttga ttttgtgagc taatgtacta gcgtcacggc caattccgtc agcgttaatt
     8041 tcagatgctt gtacaacacc gtctgggtcg ataatgaatg taccacgttg agctaaacca
     8101 gtagcttcat ctaatacatc aaaattacga gtgattgttt gtgatgggtc accaatcata
     8161 gtgtaagtga ttttgctaat tgcatctgaa tggtcatgcc atgctttgtg tacgaagtga
     8221 gtatcagttg atactgagaa tacatttacg cctaattttt gtaattcttc atattggttt
     8281 tgtaagtctt ctaattcagt tggacaaacg aatgagaagt cagcaggata gaagcatact
     8341 acgctccaag aaccttttaa atcttcttgt gtaacttctt taaattgatc tttttttgga
     8401 tcgaaagctt gcgctgtaaa tggtaagatt tctttgttaa ttaatgacat aaatatcttc
     8461 ctcctaagaa tttaagtatg aattagaact atcaattgat tgtgcttaat tataataatt
     8521 ctaatctctt agttagcatt attacatttt gatccagaat agtcaactgg ataactttgt
     8581 aaagtgaatg attactttta aaataaagaa agataatata aagtgctttg ataatggatt
     8641 ttgtagtcga tgatttaaaa ggttgtgtct atatttaata tcttgatttt aatgtaaaaa
     8701 atgtaaaaaa agaagatttg tattctcaac taagtcaacc ttattgataa tggtgtgaga
     8761 atatttgttc gagatggatg aaggtaatgc gtgagaaact ggatttttta agtacgagac
     8821 aatatttttg aaagttcaat tattaactta taagcaaata attgctataa aaaagtttgg
     8881 acgtgtacaa ttgcaatatg aagattttta attaactgta aagtatcgag gagtggtaac
     8941 gtgtcagatc atgtatataa tcttgtgaaa aagcatcatt ctgttagaaa atttaagaat
     9001 aaacctttaa gtgaagacgt tgttaagaaa ttggtagaag ctggacaaag cgcttcgacg
     9061 tcaagtttcc tacaagcata ctcaattatt ggtatcgacg atgagcagat taaagaaaat
     9121 ttacgagaag tttctggaca accttatgtt gtagaaaatg gctatttatt cgtctttgtt
     9181 attgattatt atcgtcatca tttagttgat caacatgctg aaactgatat ggaaaatgca
     9241 tatggttcaa cggaaggttt gctagtaggt gcaatcgatg cagcattagt tgccgaaaat
     9301 attgcggtaa ctgctgaaga tatggggtat ggcattgtct ttttaggatc attgagaaat
     9361 gatgttgcac gagttcgaga aattttagac ttaccggact atgtctttcc attatttggt
     9421 atggcagtag gggaacctgc agaagatgaa aatggcgcag ccaaaccacg cttaccattt
     9481 gaccatgtct tccatcataa taagtatcat gctgataagg aaacacagta tgcacaaatg
     9541 gcagattacg atcagacaat cagcaagtac tatgatcaac gtacaaacgg gaatcgcaaa
     9601 gaaacatggt cgcagcaaat tgagatgttc ctaggaaaca aagcaagatt agatatgtta
     9661 gaacaattgc aaaaatcagg cttaatacag cgatagcaag ataccaaaat aacccgcccc
     9721 ctctagctta aaatgataag tatagctaga aggggcgggt atttcttgca atgaattagt
     9781 gtgaagttaa tgcagcatta tcatttgatt cgaaagtatc tttgtcccaa tgtttagtta
     9841 acttggcggt acctgtacca gctagcattg aatcgttcac gtttaatgct gttctaccca
     9901 tgtcaatcaa tggttcaacg gagatgagca cgccggctaa agcgactggc aagtttaacg
     9961 ttgacaacac caatatggat gcaaatgtag ccccgccacc gacgccagca acgccgaatg
    10021 aactaataat cacgacagcg attaacgtta caataaattg taaatcaatt tctacattag
    10081 cgacgggtgc gaccataatt gcaagcatgg caggataaat gccagcacaa ccattttgtc
    10141 caatcgacaa tccaaatgtc gcagcgaaat tggcaatacc ctccggcacg cctagacgtc
    10201 ttgtttgtgt ttgtacattc agtggtaagg tacccgcgct tgagcgtgat gtgaatgcaa
    10261 agattaatac ttccaaagtc tttttaatat agcgaatagg gctaatgcct aacaggctta
    10321 aaataattaa gtgaatgata tacatcgtaa ttaatgcagc gtacgatgcg attaagaatt
    10381 ttcctaaagt ccaaatggcg ccaaagtcac ttgtcgataa cgtgttggcc ataattgcta
    10441 atacaccgta tggcgttaaa cgtaagacga acgtcacaat cgccattact agtgaataga
    10501 tagcgtcaat cgcacgctta agcaattcac catgatcagg ttgtttgcgt gctacgcgta
    10561 aataagcaaa tcctataaac gaagcaaata tcacaacagc aatcgtggaa gttgcacgtt
    10621 gtccagtgaa atctaagaat ggatttttag gcaataattc caaaatttgt tgtggtaacg
    10681 tatgtgctgt taaatctttc gcttgtttag caatttcgct tccacgtgct tgttcagcgt
    10741 taccaagatt aattgttgat gcgtctaaac caaacatcaa ggcatacaca acaccgacaa
    10801 ttgcagcaat ggtgacagtg ccgattaaaa agataaaaat gagactacca attttagcaa
    10861 atttttctcc gatttgaatt ttagtgaatg cagcgacaat agaaatgaaa attaaaggca
    10921 tcacaatcat ttgcaacaat gcaacgtaac cttgtccgac aatgttgaac cagtcacttg
    10981 ttgatgttat aacattcgaa tgtgttccat agacaagatg tagtaacacg ccaaatacaa
    11041 tgccgatacc taaagctgta aacacacgtt tcgcaaaaga tatatgttta cgagccatca
    11101 tgtgcaatat tacgatgaaa atcaccaata caataatatt aatcagtgta agaaaagcat
    11161 tcatgaacgt cactcctcaa atttttgaat ataattccga ccagtatact aagaataata
    11221 ttaattttaa attatttcaa gagggcattt gcaattaaat gtaaacatga tgaaattcga
    11281 ccgttggcat aaggggattt gtaaacaaat cgttttgaat tcagtagtga taggtggaaa
    11341 gggtaaatag tgtgatgagc tagtcgatta aagttaatat ttgaaaatga agcagggcat
    11401 taaatgcaat aaattaaata agttgtcatt acagcgttaa taatagaaat gattttaaca
    11461 ggaaaaaagt gatgaatatt tggaaaagat atatatcgtg tactgtcatg agagataaga
    11521 tatgagaaaa caaatgtatc cttgaatcga gatgtggcta tggcggtttg ataaaaattg
    11581 ctaacctcat atttattgaa taaaaaagat aataaatgat caaccttgaa aagacgcttc
    11641 gaacaaacac gttgtaaaaa attctttcga atcaaaatta gcatcttttg aggattcgtg
    11701 tgtgcaggtg gtagttcaaa agctaccgta aacaaatatt tttgtttgag caaaaaggcg
    11761 tttgtagtaa atgtgaatat gagtgtgccc ttgtgcgatt cgtcatttac taacacagcc
    11821 aaacatttaa attgaatggg cttattaaga tgaatctatt tagaagtaca cagaagccat
    11881 cccatttaaa agtaacatac tacaaaaatt tattcaaaat tcaatattaa tcataatgaa
    11941 taattaaaaa acctgaaaca aattagtttg atatgctctc ttcaaagtag atattgaaag
    12001 gagtttatca gattgtttca ggttttatga attgagtatt aaatttgaaa ataaattaca
    12061 gttctatagt tttataaaag atcagttttt aaatcataga tatatattac tgagtcttac
    12121 aggctgggac ataaatcaat gttctatgct ctacgaagtt atattggcag tagttgactg
    12181 aacgaaaatg cgcttgtaac aagctttttt caattctagt caaccttgcc ggggtgggac
    12241 gacgaaataa attttgcgaa aatatcattt ctgtcccact ccctacaggt tgaataaacg
    12301 tgcgaagaaa ccttttttct cttcttgagg ttttggttct gacatttcag actgtgaagc
    12361 tgtgctgtca ttttctgctt tcgcttccaa tgaatcgttc gaagtagctt gtttagaagc
    12421 ttctgcatgg gcttctgcta cttgttgttc agcatcatgt tgcgcttctg catctactgt
    12481 agcttcttta tttgaagtct cagctgcttt gtcattttta ggctgctcaa cgctggcttt
    12541 ttcaattgct tcagttgcag aaatttgatc ctctttatct actttagtag cagtgccatc
    12601 gcctgcattt tctgctttag tatccgtctt agcgtctgtt gatgtattca ctccagcaac
    12661 cttcgaatct ttactttcag ccttttcatc agtagcagta ttattttcaa ctttatttga
    12721 tgattcagcc aatactttag cttgagatgc ttgtgcttct tggatagcct cttgtgatgt
    12781 ttggatggct tttaaggatt cgctatttga atcgatttta gttgtaagtt gattttgaag
    12841 ttcttttaat tcttgttgtt gctgatgtac ttgattcatc atttgaccaa gtaggtgacg
    12901 ttcttcgcgc attttctgga tttctagacg caaatcttct actagttgag caacattttg
    12961 attagtaggt aagtttttgt catcgttttt gacaatgact tgcaggaagt ctttttcttt
    13021 ttctaattca tcaaacgcta gatcataact atttgtttgt tttactttgt cggctatgtc
    13081 tttgaaaagc tcgatatctt cttctttgaa atcagttgct tcacgaccac gatattcagt
    13141 cttgcttagt tggtaaccac gttcttctaa atgttgaaca attttacgga cttgcttctc
    13201 acttagttct acgcgttgtg caaattcttt agttaacata ctttaaagtc ctttctgcat
    13261 atactttatg tatactgacg tttgtctacg ggcagtgaaa cgtcagtctt gcttcataca
    13321 tagtttaaac gttattgtta gtgatttcaa gtttgtttaa aatggtttac gtaaatccat
    13381 ttcttctaat aaatcataaa taatttcacg tgcgatacca tttgcaaata catacttcaa
    13441 aatataattt gatttaacac ttgtaccgta gatatccttt gtcagtttaa tatttttctt
    13501 taaaaataag tccacttttt tagtttgacc atcattagtt ggatgaatgt aatcaatttc
    13561 agtttgtctt tcaaattgat gttcgtataa aaactgtcta cggtgttcaa taatgagccc
    13621 attatcttgt gttaaatctg caggttcttt agggacttcc atcattaaaa agttaatact
    13681 tctagaatga acaaggtttg ataataagtt taattcatgt tcaatttgat gtaatgtttg
    13741 tgtcattaac tcatcatggt tgggtaggtc ttttgcaaat aaataaatta aaaatgcaga
    13801 attggttgtg gcttcataat gtgcagttgc tagactgaca acaacatcgt tttctaagcc
    13861 tacgataaag acataatcgt tttcagtttt gttgttttct aatgagcgtt tgatgatacg
    13921 tttatcttca gttaaagata aatttaactt agtatcgtaa agtttaagtg cttcgttgta
    13981 atgtggatct ttgacagatt gaatggtttt aaattccata agaacacctc cccaatttaa
    14041 ataatattat agcataatcg cctgttgtaa aagactgttc ataaactttt aaatggtata
    14101 aaaaactgta ctatcttaaa ttagacagta cagtaatctc attttgaatt cagtgtgata
    14161 actaagcttt gggaccttta gatgcttcag caaaatgtgt aatatcaact tcttcataag
    14221 ctgaattatt ttctagtact tcttggtgag atgaatgatc gcgaacgcca acaactaaca
    14281 ttttatcgtc taaaataagt tgtttatatt tttctaattc atcaggcgct aagttgtagc
    14341 gtgataaaac tgcatgttca ccatcttctc ctgttaacag tttagtcatt ttatcactaa
    14401 atgttccact tgttgagata agggagattt cagagtcgtg taagtcattt aggtgtaatt
    14461 tacttttact aataattgtt agctctgatt ctaaataacc ttcagatttc ttttgattga
    14521 ttacgttgta taattcgcca gtgtcattta ctacagtaat atctgccata gttgtcgccc
    14581 ctttaaaaat ttgtttattc aatcttttac ccttcttatt ataaagtaaa acccttacat
    14641 tattaagtta taagtcttca ttcgcattaa acgtctctgt acatttataa aactattaaa
    14701 agtttaccta agcagttatt gaaaaaatgc cgaaaatttg ctattatcgt taaataattt
    14761 acataaactc atataatcta aagaatatgg ctttagaagt ttctaccatg ttgccttgaa
    14821 cgacatgact atgagtaaca acacaatact aggagtagct tcagccatta aattgtaacc
    14881 atgatgggtg atttatatca ttttatatga tggtcacagt ttatttgatg aaacttcttt
    14941 tacattgatt gcatgaccaa tacgtgatgc atgttcgttc actcataaac cctgaaacta
    15001 ttatttagtt tggggatttt tttgtatcta gcaccaattt aagagcaaaa tgtttcacac
    15061 aaatctgagg aggttttaag agtggagtta ctaggacaaa aagtaaagga agacggcgtt
    15121 gtcattgatg agaggatttt aaaagtcgat ggatttttaa atcatcaaat tgatgcaaag
    15181 ttaatggatg aagttggtcg cactttttac gagcaattta aagataaagg gattactaaa
    15241 atcttaacca ttgaagcttc cggtatcgca ccagcaatca tggctgcact gcattttgat
    15301 gtgccatgtt tatttgcgaa aaaagcaaaa cctagcactt tgacggatgg ttattatgaa
    15361 acatctattc attcatttac taaaaataaa acaagtacgg tcattgtttc aaaagaattt
    15421 ttatcagaag aagatactgt actcatcatc gatgactttt tagcaaatgg tgatgcttca
    15481 ttaggattat acgatatcgc acagcaagcg aatgctaaga cagctggtat tggtatagtt
    15541 gttgaaaaga gtttccaaaa tgggcatcaa cgtttagaag aagcaggctt aacagtttct
    15601 tctctctgca aggttgcttc actagaagga aataaagtga cattggtggg agaagaataa
    15661 tgaaaaattt aatcctaagt gttcaacatc ttttagctat gtacgcaggt gccatcttag
    15721 ttccaatcat tgttggtaca agtttgaagt ttacgcctga acaaatcgct tacttagtta
    15781 cagtagatat atttatgtgt ggagttgcca catttttaca agccaataaa gtaacaggaa
    15841 caggattacc aatcgttctt ggatgtacat tcacggctgt tgcgcccatg attttaattg
    15901 gtcaaacgaa aggaatagat gtactttacg gttcgctatt tttatcaggg atattagtta
    15961 ttatcatcgc gcctttcttt tcacatcttg tgaaattctt cccaccagta gtaacgggta
    16021 gtgttgttac tatcattggt atcaatttaa tgccagtagc aatgaattac ttagctggag
    16081 gtcaaggtgc aaaggactat ggagatgtta agaacatttt gttaggttta atgacattaa
    16141 tcattattct tgttttacaa agattcacaa ctggatttat taagagtatt gccatattaa
    16201 ttggactcgt tttaggaacg ataggtgctg gcttacttgg gatggtcgat attaatcaag
    16261 tcaatcatgc cggttggtta ggcatcccag tgccgtttaa attctcagga tttagctttg
    16321 atgtgacatc gacgttagtg ttctttattg tagctatcgt tagtttaatt gagtcgacag
    16381 gtgtctatca tgcgttaagt gaaattaccg gtaagaagtt agaaagaaaa gattttcgta
    16441 aaggttatac tgcggaaggt ctagcgatag tgttaggttc tatattcaat tcatttccgt
    16501 atacagccta ttcgcaaaat gtaggacttg tttctttatc cggcgctaag aaaaacaatg
    16561 ttatatacgg catggtcgtg ttattactta tatgtggttg tatacctaag cttggcgcat
    16621 tagcaaatat cataccgcta cctgtgttag gtggtgcgat gatagctatg tttggcatgg
    16681 taatggcata tggtgttagt atattaggac atatcgattt taaaaatcaa aacaatttat
    16741 taattatcgc tgtatcagta ggattaggta ctggtataag cgctgtacca caagcattta
    16801 aaggtttagg tgaacaattt gcatggttga cccaaaacgg aattgtttta ggcgcaatct
    16861 ctgcaattat tcttaatttc ttttttaatg gaataaagta taaacaaacg gaagaaaatg
    16921 tgaaataata taactaatta atttgaaaaa tggaggctgt ttttaatgtg ggaaagtaaa
    16981 tttgcaaaag aatcattaac gtttgatgat gtgttattaa ttccagcaca atctgatatt
    17041 ttaccgaaag acgttgattt aagcgtacaa ttatcagaca aagttaaatt aaatattcca
    17101 gttatttctg ctggtatgga tactgtaact gaatctaaaa tggcgattgc tatggctcgt
    17161 caaggtggtt taggtgttat tcataaaaat atgggcgttg aagaacaagc ggacgaagtt
    17221 caaaaagtaa aacgctcaga aaatggtgtc atttcaaacc catttttctt aacaccagaa
    17281 gaaagtgttt atgaagcgga agcattaatg ggtaaatacc gtatttcagg tgtaccaatt
    17341 gttgataata aagaagatcg caacttagta ggtattttaa caaaccgcga cttacgtttt
    17401 attgaagact tctcaattaa aattgtagat gtaatgacgc aagaaaattt aattacagct
    17461 ccagtgaata caacacttga agaagcagaa aaaattctcc aaaaacataa gattgaaaag
    17521 ttaccattag ttaaagacgg gcgtctagaa ggtcttatta ctattaaaga tattgaaaaa
    17581 gttatcgaat tccctaatgc agcaaaagat gaacatggtc gtctacttgt agccgcagca
    17641 atcggtattt caaaagacac tgatattcgt gctcaaaaat tagtcgaagc aggtgtggat
    17701 gtcttagtta tcgatacagc acatggtcac tctaaaggcg ttatcgatca agtgaaacat
    17761 attaagaaga cttacccaga aatcacatta gtagcaggta acgtagcaac tgcagaagca
    17821 acaaaggatt tatttgaagc gggtgcagat attgttaaag ttggtattgg cccaggttca
    17881 atttgtacga cacgtgttgt agcaggtgtt ggtgtaccac aaattacagc aatttatgat
    17941 tgtgcaactg aagcacgcaa acatggtaaa gctatcattg ctgatggtgg tattaaattc
    18001 tcaggagata tcattaaagc attagctgct ggtggacatg cggttatgtt aggtagctta
    18061 ttagcaggta ctgaagaaag cccaggcgca acagaaattt tccaaggtag acaatataaa
    18121 gtataccgcg gtatgggctc tttaggtgcg atggaaaaag gttcaaacga ccgttacttc
    18181 caagaggaca aagcgcctaa gaaatttgta cctgaaggta tcgaaggacg tacagcatat
    18241 aaaggtgcgt tacaagatac aatttaccaa ttaatgggcg gtgtgcgtgc tggtatgggt
    18301 tatactggtt cacacgattt aagagaatta cgcgaagaag cacaattcac acgtatgggc
    18361 cctgctggtt tagcagaaag tcacccacat aatattcaaa ttacgaaaga atcaccgaac
    18421 tactcattct aattaagata aaggagaacg acaaatatgg aaatggcaaa agaacaagag
    18481 ttaatccttg tcttagactt tggtagccaa tacaaccaat taattacacg ccgaattcgt
    18541 gaaatgggcg tttatagtga attacacgat catgaaattt caattgaaga aattaagaaa
    18601 atgaatccaa aaggtatcat cttatcaggt ggtccaaatt cagtttatga agaaggttca
    18661 tttacaattg atccggaaat atataattta ggaattccag tacttggtat ttgttacggc
    18721 atgcaattaa ctactaaatt attaggtggt aaagttgaac gtgccaatga acgtgaatac
    18781 ggtaaagcaa tcattaatgc gaagtcagat gagttattcg ctggcttacc agcagaacaa
    18841 actgtttgga tgagtcattc tgataaagtt attgaaattc cagaaggctt tgaagttatc
    18901 gctgatagtc caagtacaga ctatgcagca atcgaagata agaaacgtcg catttatggt
    18961 gttcaattcc atccagaagt acgtcataca gaatatggta atgatttatt aaataatttt
    19021 gtccgtcgtg tttgtgattg taaaggtcaa tggacaatgg aaaactttat cgaaatcgaa
    19081 attgaaaaga ttcgtcaacg cgtaggagac cgtcgtgtat tatgtgcgat gagtggcggc
    19141 gtagattcat ctgttgtagc tgtactattg cataaagcaa taggtgatca actaacatgt
    19201 atctttgtag accatggctt acttcgtaaa ggtgaaggcg atatggttat ggagcaattc
    19261 ggtgaaggtt tcaacatgaa tattatccgt gttaatgcga aagatcgctt tatgaataaa
    19321 ttaaaaggtg tttcagatcc tgaacaaaaa cgtaaaatca ttggtaatga atttgtatat
    19381 gtgtttgatg atgaagcatc aaaacttaaa ggtgtagact tccttgcgca aggaacacta
    19441 tatacagacg tcatcgaatc aggtactaag acagcacaaa caatcaaatc acatcacaat
    19501 gttggtggat taccagaaga catggaattc gaattaatcg aaccaatcaa tacattgttt
    19561 aaagatgaag tacgtaaatt aggtattgag ttaggtattc cagaacattt agtatggcga
    19621 caaccattcc caggacctgg tcttggtatt cgtgtacttg gagaaattac tgaagataaa
    19681 ctagaaatcg taagagaatc agacgcgatt ttacgccaag tgattagaga agaaggtctt
    19741 gaaagagaaa tttggcaata cttcacagtg ttaccaaaca ttcaatcagt aggtgttatg
    19801 ggagactacc gtacgtatga tcacacagta ggtatccgtg cagtaacatc tatcgacggc
    19861 atgacaagtg acttcgcacg catcgattgg gaagtcttac aaaagatttc tagtcgtatc
    19921 gtaaacgaag tagatcacgt caaccgcgta gtctatgaca ttacatcaaa accaccaagc
    19981 acaattgagt gggaataatt atatata
//
LOCUS       DATTQF010000031        19952 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.031, whole genome shotgun sequence.
ACCESSION   DATTQF010000031 DATTQF010000000
VERSION     DATTQF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.031"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            26..403
                     /locus_tag="VB241_001030"
     CDS             26..403
                     /locus_tag="VB241_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1244334.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            554..1336
                     /locus_tag="VB241_001031"
     CDS             554..1336
                     /locus_tag="VB241_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEP1244335.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1462..2193)
                     /gene="erm(A)"
                     /locus_tag="VB241_001032"
     CDS             complement(1462..2193)
                     /gene="erm(A)"
                     /locus_tag="VB241_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEP1244336.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2251..2310)
                     /locus_tag="VB241_001033"
     CDS             complement(2251..2310)
                     /locus_tag="VB241_001033"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEP1244337.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2702..3364
                     /locus_tag="VB241_001034"
     CDS             2702..3364
                     /locus_tag="VB241_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP1244338.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3596..3745
                     /locus_tag="VB241_001035"
                     /pseudo
     CDS             3596..3745
                     /locus_tag="VB241_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3813..4097
                     /locus_tag="VB241_001036"
     CDS             3813..4097
                     /locus_tag="VB241_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244339.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIITGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(4213..4410)
                     /locus_tag="VB241_001037"
     CDS             complement(4213..4410)
                     /locus_tag="VB241_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244340.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            4570..5043
                     /locus_tag="VB241_001038"
     CDS             4570..5043
                     /locus_tag="VB241_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HEP1244341.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKLEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            5117..5236
                     /locus_tag="VB241_001039"
     CDS             5117..5236
                     /locus_tag="VB241_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244342.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNR"
     gene            5436..6278
                     /gene="mreC"
                     /locus_tag="VB241_001040"
     CDS             5436..6278
                     /gene="mreC"
                     /locus_tag="VB241_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEP1244343.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            6278..6808
                     /gene="mreD"
                     /locus_tag="VB241_001041"
     CDS             6278..6808
                     /gene="mreD"
                     /locus_tag="VB241_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEP1244344.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRSFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    6835..7088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            7101..7409
                     /gene="rplU"
                     /locus_tag="VB241_001042"
     CDS             7101..7409
                     /gene="rplU"
                     /locus_tag="VB241_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP1244345.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            7415..7735
                     /locus_tag="VB241_001043"
     CDS             7415..7735
                     /locus_tag="VB241_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEP1244346.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            7747..8031
                     /gene="rpmA"
                     /locus_tag="VB241_001044"
     CDS             7747..8031
                     /gene="rpmA"
                     /locus_tag="VB241_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP1244347.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            8518..9810
                     /gene="obgE"
                     /locus_tag="VB241_001045"
     CDS             8518..9810
                     /gene="obgE"
                     /locus_tag="VB241_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP1244348.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLILFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            9823..10278
                     /locus_tag="VB241_001046"
     CDS             9823..10278
                     /locus_tag="VB241_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEP1244349.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            10292..10894
                     /gene="ruvA"
                     /locus_tag="VB241_001047"
     CDS             10292..10894
                     /gene="ruvA"
                     /locus_tag="VB241_001047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP1244350.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTEQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            10926..11930
                     /gene="ruvB"
                     /locus_tag="VB241_001048"
     CDS             10926..11930
                     /gene="ruvB"
                     /locus_tag="VB241_001048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP1244351.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            11932..12957
                     /gene="queA"
                     /locus_tag="VB241_001049"
     CDS             11932..12957
                     /gene="queA"
                     /locus_tag="VB241_001049"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP1244352.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            12980..14119
                     /gene="tgt"
                     /locus_tag="VB241_001050"
     CDS             12980..14119
                     /gene="tgt"
                     /locus_tag="VB241_001050"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP1244353.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            14138..14398
                     /gene="yajC"
                     /locus_tag="VB241_001051"
     CDS             14138..14398
                     /gene="yajC"
                     /locus_tag="VB241_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP1244354.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            14673..16952
                     /gene="secDF"
                     /locus_tag="VB241_001052"
     CDS             14673..16952
                     /gene="secDF"
                     /locus_tag="VB241_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEP1244355.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQYWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKGSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            17155..19428
                     /gene="recJ"
                     /locus_tag="VB241_001053"
     CDS             17155..19428
                     /gene="recJ"
                     /locus_tag="VB241_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP1244356.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEQFNRERKDIVATITEEAMAMA
                     EMKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            19450..>19952
                     /locus_tag="VB241_001054"
     CDS             19450..>19952
                     /locus_tag="VB241_001054"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP1244357.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSL"
BASE COUNT         7122 a         2747 c         3798 g         6285 t
ORIGIN      
        1 tggaagtttg acggggtaat tatcaatgga taaacaagtt agaaatacaa cagaaattgt
       61 acgtttggcg aagcagaaat caaaaaagac aagggaaaaa gtagacaaag cgatttctaa
      121 attttcgatt gaaggtaaag ttattaattt taattcaata gcaaaggaag ctaatgtttc
      181 taaatcatgg ctttataagg aacacgatat taggcaaaga atcgaatccc ttcgtgagcg
      241 tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg aggaaatcct
      301 tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa gaaaataaaa aattacagaa
      361 ccaaattcaa aaattatatg gagatctgta taataaagaa taattattaa tctgtagaca
      421 aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt attgaacagt aatttaagta
      481 tatgtccaat ctagggtaag taaattgagt atcaatataa actttatatg aacataatca
      541 acgaggtgaa atcatgagca atttgattaa cggaaaaata ccaaatcaag cgattcaaac
      601 attaaaaatc gtaaaagatt tatttggaag ttcaatagtt ggagtatatc tatttggttc
      661 agcagtaaat ggtggtttac gcattaacag cgatgtagat gttctagtcg tcgtgaatca
      721 tagtttacct caattaactc gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa
      781 gattggaaat acggattctg ttagaccact tgaagttacg gttataaata ggagtgaagt
      841 tgtcccttgg caatatcctc caaaaagaga atttatatac ggtgagtggc tcaggggtga
      901 atttgagaat ggacaaattc aggaaccaag ctatgatcct gatttggcta ttgttttagc
      961 acaagcaaga aagaatagta tttctctatt tggtcctgat tcttcaagta tacttgtctc
     1021 cgtacctttg acagatattc gaagagcaat taaggattct ttgccagaac taattgaggg
     1081 gataaaaggt gatgagcgta atgtaatttt aaccctagct cgaatgtggc aaacagtgac
     1141 tactggtgaa attacctcga aagatgtcgc tgcagaatgg gctatacctc ttttacctaa
     1201 agagcatgta actttactgg atatagctag aaaaggctat cggggagagt gtgatgataa
     1261 gtgggaagga ctatattcaa aggtgaaagc actcgttaag tatatgaaaa attctataga
     1321 aacttctctc aattaggcta attttattgc aataacaggt gcttactttt aaaactactg
     1381 atttattgat aaatattgaa caatttttgg gaagaataaa gcgtcctctt gtgaaattag
     1441 agaacgcttt attactttaa tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa
     1501 ttgttccttc gatagtttat taatattagt gacatttgca tgcttcaaag cctgtcggaa
     1561 ttggttttta gtgaaaagaa cacgatattc acggtttacc cacttataaa caaaagatcg
     1621 atactttttg tagtccttct ttgaaatcaa tggttgatgt cgttcaagaa caatcaatac
     1681 agagtctaca cttggcttag gatgaaaata tagtggtggt acttttttga gcatttttat
     1741 atccatctcc accattaata gtaaacccaa agctcgttgc agattttgca atcttttcgc
     1801 aaatcccttc tcaacgataa gatagctata tttagcctga ctttcaaagg taattctttt
     1861 gacaatatcc gtactgatgt tataaggaat attaccatat atcttatagt ttatatgttt
     1921 tgggaaggaa aattttagaa tatccgtttg aatcactttt atattctcag aggggtttac
     1981 cgcttcttta gtcacttgac ataagcctcc atcaatttct atagcagtaa ctgatcgact
     2041 cattttgact agctctttgg taaaatgtcc ttttcctgat ccgatttcta ttacgttgtc
     2101 ttgtttactg atattcgtgt gattcaatat ttcttttaca tgctttttag aagtaataaa
     2161 attttgcgtg tctttagggt ttttctggtt cattataacc ttctccttac tggttataat
     2221 gaactgacat taagcgttca ttataaccaa ttaattttga tttggttgat aacgaacttt
     2281 attaataaca aatatagaaa aagtacccat acttttatct cctttccatt tttttcatta
     2341 tgaatgagat aaagtaattt ctactactgc gatactggtg cacataattg aaacctcctt
     2401 atgtaactga atatgattgt atcatctttt cattttagaa taaaccttta tatgtaattt
     2461 tgcacttgac acttcagatg gacgttttct tcaaaataat atgttttgaa acttgataag
     2521 agaagccact gtctttacta aaaagtaaag gctgcgataa aggacagtga ctttaataaa
     2581 gttgcactgt tgtattgtct ttggataaaa ttgtctaaag ggaagtttta tccccaacga
     2641 aaaaacatct aaagtggtaa gtaatgaaag aatgtcgata aagtaggaat gggggatctt
     2701 tttgaataca atagaatcac agtttgataa ggttgcagaa gattacgatt tcgtgaatga
     2761 gcttttgaat gattattcat tctttgtgtc taatatgtct ccaaagaaag gcagagcatt
     2821 agatatcgga tgtggctcgg gtttgttagt ggagaaatta gcaagttatt atgatgaagt
     2881 ggtagggatt gatatttcta atcaaatgct cgatcttgcc aaatctaaac gtcaactaac
     2941 aaatacggtc tatctgaata tgaatgcaga acaacttaat tttaatgaga agtttgattt
     3001 tattgtaagc cgaacaacct ttcatcattt ggatgatata gccagcgtaa tacagcaaat
     3061 gaaggaactg ttgaatgagg aaggaagaat agttattctt gataatgtat ctgaagtcga
     3121 gacaccgcct acctatgttt ataaattagg ggcaatccaa gagtttttac cgcattgttt
     3181 caaatttgga ataaaaaatg cgattagaat ttataatcac aacacatcta aatcatggct
     3241 tgagcattta gcttcagata agtacctatc tgaacaaaat tattacgatt tatatgaaaa
     3301 gttgttgcct ggatgccaat tccataaaat gggttgggca atgggggttg tctggacaaa
     3361 ataacttaaa gctgtggctt tgaaaagttg aatttgtgat tgttttcaga gctatcgggt
     3421 gctttagtat aaataacaat gaataaaaaa tcagcctgta tcttatgtta ctattaagta
     3481 atttaagata caggttttac tatttttgaa ataagtacct tccacttcaa atacatgtat
     3541 tcaaagtgaa agtattgatt taaatgattc ttttgtaatt tactcttgat aaattttaac
     3601 gccctcacaa gaagatataa taacaacaat gaggttgaag gagtgtggtt tgattttagg
     3661 aatagattta ttggatcata ttataatcgg tgataataga tttaccagtc ttgtagaagc
     3721 gggttacttt gatgaaaatg attgaagttg atagaattaa tgacgtatct tgtgtataat
     3781 acctacgaag tactttcatt ggaggaaaaa tagtgactct atttattatt atcggggttc
     3841 tcgtgccaat ggtttatacc atgcagttaa atattaaaaa tgaacctgta acaaagcgca
     3901 atctattaat aacattagct ttatctacgt taggtatttt agtaacggca ttagcaggtg
     3961 tgattgttac gaaacaagct tttcctttat taagtgtagc aataggctca attattactg
     4021 gaatcgtttg gggcctatta ctaagtggta gctacgcgct gatacgattt ttatctaacg
     4081 catttgggcg taaataatag taatctgttc atcaagtagt atacgtgctt gaaaacaaat
     4141 taaactccta atgtggaact agttaaaaaa ttctaaacta gtacattagg agttttgtta
     4201 tgcagaatag atttaattgt taaattgaaa gtgtggtaaa aatccactat ttatttgaaa
     4261 aaaatcgagc aaccatatta agccttgata tcctaagtaa atacataata aaacaagtcc
     4321 gacataaatt aaaaatcgca aaatagaaag tccaactcta aaaaggagga tgactaatag
     4381 tgctattaaa ataattgtga gtatactcaa tgctacaaac ctcctcatac gcttttaaat
     4441 cataacaaaa ttataagcca ttttaaatga acgtgcatca gtcctaagat atatcatgat
     4501 taaggctgaa gactgaggaa aatatacata ataaaatata taattagatg aagacattgg
     4561 aggcgaacta tgagattgat atttagtatg attaaaaaca ttatagcagt aatagcaatt
     4621 gtgataattg tttatatcgc cttgaaatac gcaccttttt taaaagaaca agattggaat
     4681 cctgtaaatc acgataatga tcaaatgaat caagttacgc aacccactaa tgatgcccaa
     4741 catgtttatg taagtggtga gaaatattca ttaaaagaaa atgatttaat aaaaaatgta
     4801 ccattaagcc aaattaaaaa tgtatttaaa atgatagata aactagagtt tatggctgtg
     4861 tctggaatga atcgcatggc ttataatgat caatatatta taggtcaaag aggagacgaa
     4921 tttattcttt ataaatttgg agatgagtca atgcgtgttt acaatactga atttgaaatg
     4981 caacaagact taaatgaatt agggcaaaat ttacaactaa aacccgaaaa tgcttatcaa
     5041 taggaaatta ttgcataaga aatatatttt agttatcctg tcactagaat aaatttaaaa
     5101 ggttggtgta gttcaaatgg cagaacaatc aaaacagaaa caagctaatg aacaacaaaa
     5161 ggcccagaac ttattcgcac gttggagaca acttcagaat tcgaacagtg aatcctcgaa
     5221 cgatacaaac agataatttt aaaatttaaa tcgattattt aaactactag tgactggaat
     5281 gtttgctagt agttttttgt ttttaaaatg ccacaaattt ttttacggag ttgttcattt
     5341 tcaaagtaat tcgtagttag tgctttattt cataatggga gtaatataaa atagaaaagg
     5401 atatacaata ttttgacata atagaggtgt tctgggtgct taagtttttt aaaaataaca
     5461 aattaattgt tgttttatgt gcaattatcg tttttattgc attaattggg ctgtccatac
     5521 gttcacaatc tcaatcacct cctgaacaat atataggtga ttctgtgtct tttggacaac
     5581 gagttgtgag ttatccagtt aattttgttg ctggtacgat tggggacttt tttaaaaaag
     5641 gagattctaa agaatctaaa aataagatta gccagttaga atctaagaac caacaattag
     5701 aagcggaaaa tgaaaaatta aaaaaagagc ttgatttaaa agatatttca aaatttgatc
     5761 ctatttctac tacggtttta gcaagaaatc cggatcagtg gatgaataca attgtaattg
     5821 ataagggatc taaatcaggt ataacttcaa atatggctgt gatgacatca caaggttttg
     5881 ttggaagagt tactaaagtt aataaatttt cttcacaagt tgatttaatc tcaactaata
     5941 cacgtgcggg taaattatct gtaaatatac aacacggttc taaaaatata tttggcttaa
     6001 ttgatcgtta tgatgaaaag aactcagaac ttgtaattag tgatattaat aatagagata
     6061 atatctcaaa aggtgataaa gtcgttacaa gtggattagc tgatcaacta ccaagtaatt
     6121 tatatatagg agaagtgact aaggttcaaa atgatcaata cggcttagct aaagaagtta
     6181 gggttaagac tggtgcagac ttaacagatt tgagccatgt ttatgttgca aaaagagatc
     6241 ctaaaacaat tcctgatgat gaaagcaggg ataaataatg cgtacactgt attatttttt
     6301 gataggtatt ttactatttt atatagatac tgcaattggg cttcttatac caatgcacat
     6361 aggtaagttt gaacttgtat ttgtaccaca ccttacattt atgtatattt taatgatggt
     6421 agtgtatcgt agctttggcg tatcattatt gctcagcata tttttaggtg taatgactga
     6481 tgtatacttt ggtagtattt atggagtgta cttatttggc tatatattgt ttttagcact
     6541 tatagatcga ttctttaaaa ttttttacaa agatcattcg atgttattca ttattatatt
     6601 agctagcacc ttattattag aagtctatgt ggcattaata tacggtatgt taggattcat
     6661 tcaatttgat attattcatt ttgtagtctt tagattattg ccaacattaa ttatgaattt
     6721 tgtactgttg atcatgcttt atccgttgat tataaagttc cttaaaaaaa caaacaatga
     6781 cattgacatg aaacgccgtc aatggtaaga tgcaatagtt gagtagttat aagtagctac
     6841 atacaaccgc tcaaatatag gttcaagaac atttttaatg aagaaagcga ttttcccaca
     6901 gagtgcaaac gcttgactca tttgcagaag ttattgaaag aaagcgattg tcaatcaagg
     6961 cgttgaagga agaaacgaga agagtttatc tttataaatg actcgagttg cgaccgaaac
     7021 aacgtagagt gcaaacgctt gactcaataa cgaaatgtca cctatgtatg gcgtgactta
     7081 ttatacagga ggtgcaaagt atgtttgcta ttattgaaac aggtggaaaa caaatcaaag
     7141 tagaagaagg tcaagaaatc ttcgttgaaa aattagacgt aaacgaagga gatactttta
     7201 catttgataa agtattattt gtaggtggag attcagttaa agttggagcg ccaacagttg
     7261 aaggtgcaac agttactgct actgttaata aacaaggtcg cggtaaaaaa atcactgtat
     7321 tcacatacaa acgtcgtaaa aattcaaaac gtaaaaaagg ccatcgtcaa ccatacacta
     7381 aattaacaat cgataaaatc aacgcgtaat tattatgatt actgttgata ttacagttaa
     7441 tgatgaaggc aaagtaacag acgttattat ggatggccat gctgaccatg gtgaatatgg
     7501 tcatgatatc gtttgtgctg gagcttcagc tgtattgttt ggtagtgtta atgcgattat
     7561 aggattgaca tctgagagac cagatatcaa ttatgacgac aatggtggtc attttcatat
     7621 aagaagcgtt gatacaaata acgatgaagc gcaactaatt cttcaaacaa tgcttgtgtc
     7681 tttacaaact attgaagaag aatataatga gaatattaga ttaaattata agtgaggtgc
     7741 attccgatgt taaaattaaa cttacaattc ttcgcatcta aaaaaggggt aagttctaca
     7801 aaaaacggac gtgactctga atcaaaacgc ttaggtgcta aacgtgctga cggtcaattc
     7861 gtaacaggtg gttcaatttt atatcgccaa cgtggtacta aaatttaccc tggtgaaaat
     7921 gtaggtcgtg gtggcgatga tacattattc gctaaaatcg acggcgttgt taaattcgaa
     7981 cgtaaaggtc gcgacaaaaa acaagtttct gtatatgcag tagctgaata attttgtcta
     8041 gttaacacca gaagtgaatc ttctggtgtt ttttactttt tataaaacgt ttttcattat
     8101 tattttcatt ttgttattaa aaaatgggaa ttatagacat tatactaaat gtgcaactgc
     8161 ataagagccc ctaatcgcta aactataagg ggacaaagga atacagttgc agtataaaaa
     8221 gcaaaaagta tcgaatttgg tactcggaaa tatcagcaat ttgagtaaag taaacttaaa
     8281 agcaactgca taagagcccc taattattta caatatataa ggggctaaaa ggattacagt
     8341 tgcagcgttt aaagaataaa ctgtaccaca attggtgttg agaaatataa gtatcttaaa
     8401 gcaaagattt ataaaagcaa ctgcataaga gcccctaatt atttacaata tataaggggc
     8461 tctaatatgc tataattatt gggaaaatga aaattatatg taaaagaggt gagatatatg
     8521 tttgtcgatc aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg tattaccgca
     8581 tacagaagag aaaaatatgt accatttggt ggaccagctg gcggtgacgg tggtaaaggt
     8641 gcttcagtcg tatttgaagt ggatgaaggt ttaagaacgt tattagattt taggtatcaa
     8701 cgtcatttta aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca tggtaaaaat
     8761 gcggaagatt tagtattaaa agttccacct ggcacaatta ttaaaaatgt tgaaacagat
     8821 gaagtgttag cagatcttgt tgaagatggt caaagagctg tagtagcgaa gggcggtcga
     8881 ggtggccgag gtaattcacg ttttgcaaca cctagaaacc ctgcacctga cttcagtgaa
     8941 aaaggtgaac caggtgagga attagatgta tctttagaat tgaaattatt agctgatgta
     9001 ggattagtag gtttccctag tgtgggtaaa tcgactttat tatctatcgt ttcaaaagct
     9061 aagcctaaaa taggggcata tcattttaca acgattaaac caaatctagg agttgtttca
     9121 acacctgatc aacgaagttt tgttatggca gatttaccag gtttaattga aggtgcatct
     9181 gatggcgttg gattaggaca tcaattttta agacatgtag agagaacaaa agttattgtt
     9241 cacatgattg atatgagtgg ttctgaaggt agagagccta ttgaagatta taaagtcatt
     9301 aatcaagaat tagccgcgta cgagcaacgt ttagaagata gacctcaaat cgtagtagct
     9361 aacaagatgg atttacctga atcacaagat aatttaatct tgtttaaaga agaaattggc
     9421 gaagatgtgc cagttattcc agtttcaaca ataacgcgtg ataatattga tcaattatta
     9481 tatgcaatag cagataaatt agaagaatat aaagatgttg acttcacagt tgaagaagag
     9541 gagtcagttg gcattaaccg agtattatat aaacatacac cgtcacaaga taaatttaca
     9601 atttcaagag atgatgatgg tgcttatgtg gtaagtggta atgctattga aagaatgttt
     9661 aaaatgactg actttaacag cgatccagca gtacgtcgat ttgctcgtca aatgcgttcg
     9721 atgggtattg atgatgcact tagagaacgt ggttgtaaaa atggtgatat cgttagaatt
     9781 cttggcggag aatttgaatt cgttgaatag gagcgtaaca tgatggacaa taaagattat
     9841 aaaaagtttt atttaattag agaagatgtc ttgcctgaat ccgtggttaa aacattgaag
     9901 attaaagatg ccttaaaaag tgatccgaca ttgtccattt atgatgccgt taaacagttt
     9961 gatctatcta gaagtgcttt ttataaatat agagaaacga tatttccagt agacgataaa
    10021 atgcttgacc atcgagaatt tacattaatt ttatatgtaa ctgatattgt gggtatgttg
    10081 gcacgtgtac tagatgttat atcaaagtta gaactatctg tattaacgat tcatcaaagt
    10141 attccaatgg aagaaaaagc aacaataaca ttatcactga atgctaaatc taaagaaact
    10201 tcagtagaag atgttattgg cgctttgaga aatttagatt atgtatcaaa agtagaatta
    10261 attagtatga gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag gtaagttaac
    10321 acatttatat cctacacacg tagttgttga aactgctggt gttggttatg aaattcaaac
    10381 accaaattct tatcgttttc aaaagcatct agatcatgaa gttttaattc atacatcttt
    10441 aattgttcgt gaagatgcac aattattgta tggatttagt agtgaagaag agaaagatat
    10501 gttcttgagt ttaattaaag tcactggtat tggtccgaaa tcagctttag ctattttagc
    10561 gacaagtaca cctaatgaag taaaacgtgc cattgaaaat gaaaatgata cgtatttaac
    10621 taaattccca ggaattggta agaaaacggc aagacagatt gtcttagatt taaaaggtaa
    10681 agtgaaaatc actgaagaag atagcgattc attattacaa gtagacgcta catcgacgga
    10741 gcaagatcaa ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt attctaaacg
    10801 agagcttgca aaagttgaga aaacgttaaa taaaaataaa tatgactcag ttgatgaagc
    10861 tgttaaggca ggtcttcaat tagttgtatc ttaattttaa atagattaat aggggaagtg
    10921 ttgtcatgaa tgagcgtatg gttgatcaat caatgcatag tgaagaaact gatttcgaat
    10981 tgtcgcttag acctacgaga ttacgacaat atattggtca aaattcaata aaaagtaatt
    11041 tagaagtatt tattaaagcg gctaaacttc gtcatgaacc attagatcat gtattgcttt
    11101 ttggcccccc tggattaggt aagacaacat tatctaatat cattgccaat gaaatggaag
    11161 ttaatatacg tacagtatca gggccttcat tagaaagacc tggtgatttg gctgcaattt
    11221 tatcagggct tcaacctgga gatgttttgt ttattgatga aatacacaga ctgagtagtg
    11281 ttgttgaaga agtgctatac cctgcaatgg aagatttctt tttagatatt atcattggta
    11341 aaggcgatga ggctagaagt atccgtatcg acttacctcc attcactttg gtaggtgcaa
    11401 caacacgagc tggtagctta acaggtccac taagggatcg atttggtgtg cacttaagat
    11461 tagaatatta taatgaatca gatttaaaag aaatcattat tagaacagct gaggttttag
    11521 gcacaggtat tgatgaagaa agtgctattg aacttgctaa acgttctaga gggactccaa
    11581 gagtagccaa tcgactattg aaacgggtaa gagacttcca gcaagtgaat gaagatgaac
    11641 aaatatacat tgaaacaaca aagcacgcat taggtttact tcaagttgat caacacggac
    11701 tagattacat tgatcataaa atgatgaact gtattattaa gcagtataat ggtggacctg
    11761 ttggtttaga tacgattgcc gtaacaattg gcgaagaacg tattacaatt gaggacgttt
    11821 atgagccatt tcttattcag aaaggctttt tagaacgtac gccgcgtggc agaaaagcaa
    11881 caccattagc ttatgaacat tttgcaaagt cgaatgagga gagagaataa cgtgaatatt
    11941 gaagaatttg actatgactt accagaatca ttaattgctc aaacgccttt aaaagatcgt
    12001 gatcatagtc gtttattagt catggataga gaaactggtg aaatgaaaca tttacatttt
    12061 aaagatatca ttgagtattt tagacctggt gatacattag tgcttaacga tacgcgagta
    12121 atgccagcta gactttttgg tttaaaagaa gaaactggtg caaaagttga aatgttaatg
    12181 ttaactcaaa ttgaaggtaa tgattgggaa gtcttactga aaccagctaa gcgtattaaa
    12241 gttggtaata aattgaattt tggtaatggc aaaattatag ctgaatgcat aaaagaaatg
    12301 gatcaaggtg gacgtatcat gcgtttacat tatgaaggta ttttacaaga aagattagat
    12361 gaattagggg aaatgccact gccaccatac atcaaagaac gtttagatga tccagatcgt
    12421 tatcaaacag tttacgctaa agaaagtggt tcagcggcag caccaacagc tggattacat
    12481 tttactgatg agttattaac tgaaattaaa aataaaggtg ttaatatcgc atttgttaca
    12541 ttacatgttg gattaggtac gtttagaccg gtgagcgtcg acgatgtgaa tgaccacgaa
    12601 atgcatagtg aatattatca aatgacacaa gaaacagctg atttattaaa tgatactaag
    12661 tccaaaggac atcgcattat atcagttggt acaacttcaa cacgtacact tgaaacaatt
    12721 cgacgcgatc atgataaatt tgttgaaacg agtggctgga ctaatatatt tatttatcca
    12781 ggatttgatt ttaaagctat tgatggccag attactaatt ttcatttacc aaaatcaaca
    12841 ttagttatgc tagtatcagc gtttagtact cgtgaaaatg ttctgaatgc ttataaaacg
    12901 gcagtaaatt tagaatatag attctttagt tttggcgatg caatgttaat tatataaaaa
    12961 gaatgtgagg attttgaata tgcctgcagt aacatacgaa cacattaaaa cttgtaaaca
    13021 atcaggtgcg cgtttaggta tcgtgcacac accacacggt tcatttgaaa cacctatgtt
    13081 tatgccagtt ggtactaaag caaccgttaa aacaatgagt ccagaagagt taagacaaat
    13141 tgaagcaaaa atcattttgg gcaacacata tcatttgtgg ttacaacccg gaaatgatat
    13201 tatcaaacac gctgggggat tacataaatt catgaattgg gatggtccga ttctaacaga
    13261 ttcaggcggt ttccaagtgt ttagtttaag taatttacgt aaaattacag aagaaggcgt
    13321 ggaatttaga catcatacta atgggtctaa attatttttg agtcctgaga aatcaatgca
    13381 aattcaaaat gatttaggtt ctgatattat gatggcattt gatgaatgtc caccgatgcc
    13441 tgctgaatat gattatgtaa aaaaatctat tgaacgtaca acgcgttggg cgaaaagatg
    13501 tctagatgca caccaaagac ctgaagatca agcattgttc ggcattatac aaggtggcga
    13561 atatgaagat ttaagagaac aaagtgcaaa ggatttagta gaattagatt ttcctggtta
    13621 tgcaatcgga ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa tggttgaaca
    13681 tacagagcag tttatgccta aagataaacc aagatattta atgggtgtag gatctccaga
    13741 tgcgttaatc gaatgtagta ttcgcggcat ggatatgttt gattgtgtct taccgacacg
    13801 tattgccaga aatggtactt gtatgacatc gcaaggtcgt ttagttatta aaaatgcaaa
    13861 atttgcagat gatttaagac cgttagatga gaattgtgac tgttatacat gtcaaaacta
    13921 ctcaagagcg tatatacgtc atttaatcaa ggcagaggaa acttttggta ttcgtcttac
    13981 tactattcat aatttacatt ttctgctaaa attaatggaa gatataagac aagccattcg
    14041 agaagatcgt cttttagatt tcaaagaaga attcttcgag caatatggat taaatgttga
    14101 gaacccaaaa aacttttaag caagaggagc gtataaaatg caattttcat tacttatata
    14161 tatagtcgta atttttgcgg ttatgtattt cttgatgatc agaccacaac aaaaacgtgc
    14221 gaaacagcat cgtgagttga ttaataacat tcaatctggt caaagaatta caactattgg
    14281 tggtattaaa ggtactgtta aagcagtaga tgaaacaact gttgttatta cagttaatgg
    14341 tcatggtact gaattaactt tcgaaaaacc tgctattaaa caagttgacc cttcataaca
    14401 agaagtggta ttaaaaagga tgaaacgaat agcgcttcat cctttttaag ttataagtag
    14461 tatgtagaat agcttatttt tattccaaaa aaagaaattg tgatacataa taattgttaa
    14521 atttaaatta aatatatcta tgactatttt tactccctgc tctagtatgt taatatgtat
    14581 aaggctaata aattttgtaa gaaagattta acttgcgtta taaaatagta ttcaagtatt
    14641 ctaaatacat acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga ataattgcgt
    14701 tcttgctttt agtcgtattg ttgtttgccg gaatggctgc aacttataaa agtgttataa
    14761 agaatgtcaa tttaggactt gatttgcaag gtggatttga ggtgctttat caagtcgacc
    14821 ctttaaataa aggcgacaaa atagataaaa aagccttaca atcaacagcg caaacattag
    14881 aaaatcgtgt taacgtttta ggtgtttccg aacctaaaat tcaagttgaa gaacctaata
    14941 gaattagagt tcaacttgct ggtgtaacag accagaatga agcacgtaaa attttatctt
    15001 ctcaagctaa tttaacaatt cgcgatgcag aagataaagt aaaattaagc ggttctgata
    15061 ttaaacaggg atctgccaaa caagaattta aacaggaaac aaaccagcca acagttactt
    15121 tcaaagtaaa agacaaaaac aaatttaaaa aagtaacgga agaaatctct aagaaacgtg
    15181 ataatgtcat ggttgtttgg ttagatttca aaaaaggtga cagctataaa aaagaagcac
    15241 aaaagaaaaa ccctaaattt atttctgcag caagtgttga tcaaccaatc aattctgata
    15301 gcgtagaaat ctctggtgga tttaaaggac aagaaggcgt taaaaaagca aaacaaatcg
    15361 ccgaattgtt aaatgcaggt tctttaccag ttgacttgaa agaaatttat tcaaactcag
    15421 taggtgccca atttggtcaa gatgcattag ataaaactgt gtttgcatcg tttattggtg
    15481 tagcattaat ttatttattc atgcttggat tctaccgatt acctggttta gtagcgatta
    15541 ttgcattgac aacttatatc tatctaacgt tagtagcatt taatttcatt tccggggttc
    15601 taactttacc aggattagcg gcgttggtat taggtgtagg tatggctgta gatgccaata
    15661 ttatcatgta tgagcgtatt aaggatgaac ttcgaatagg tagaacgata aagcaagcct
    15721 tttctaaagc aaacaaaagt tcgttcctaa caatttttga ttctaactta acaacagtta
    15781 tcgccgcagc agtattattc ttcttcggtg aaagttcagt taaaggtttc gcgacaatgt
    15841 tattattagg tattctaatg atctttgtta cagccgtgtt cttatcaaga ttcttattat
    15901 cattacttgt ttcatcaaat atattcaaaa atcaatattg gttatttggt gttaaaaaga
    15961 ataaacgaca tgatattaat gaaggtgtag atgttcatga ccttaaaact tcattcgaga
    16021 aatggaattt tgttaaatta gctaagccat taattggagt aagtatttta attgtagttg
    16081 tcggtttagt tattctttat atcttcaagt taaacctagg aattgatttc tcaagtggta
    16141 cacgtgtaga tttccaatct aaacaagcaa ttactcaaca aaaggttgag caagttgtaa
    16201 aaggtagtgg acttaaagcg gatcaaattc aaatcaatgg taaagataat aaagtagcaa
    16261 ctgttcaatt taaagacgat ttaacgcgtg ctcaagataa taaattgagt gataatatta
    16321 aatcgaaatt cggtgacaca cctcaaatta acactgtatc accaattatt ggacaggaat
    16381 tagcaaaaaa tgcaatgctt gcattaatct atgcatcgat aggtatcatc atctatgtat
    16441 cattacgatt tgaatggcgc atgggtcttt catctgtatt ggcattatta catgatgtat
    16501 tcattatagt agcgattttc agtttattta gaattgaagt agatttaaca tttatcgccg
    16561 ctgtattaac aattgtcggt tattcaatta atgatacaat cgtaacgttt gaccgtgtac
    16621 gtgaaaactt acaaaaggtt aaagtgatta ctacaacaga acaaattgat gatatcgtta
    16681 atagatcaat tagacagaca atgacacgtt caattaatac agtattaaca gttattgtag
    16741 tagtagttgc tatactattc ttcggtgctc ctacgatatt caactttact ttagcattat
    16801 ttatcggatt gatttctggt gtattctctt caatcttcat tgccgttccg ctttggggaa
    16861 taatgaaaaa acgtcagttg aaaaaatcgc cgaaacacaa attagttgta tataaagaaa
    16921 agaaatcgaa cgatgaaaag attttagttt aaaatgaatt aagcggtatg tgaaacatta
    16981 aagagttcac ataccgcttt ttactttgtt taaatttaac aaaatatata taaataagtt
    17041 cccgcattca tttttaaaaa atggtataat taaagtcttg attatttaga taagtgcttt
    17101 ttatttaaag taattttaga tataatgtct tttggaaatg aggcgtaaat atatatgatt
    17161 aaaccgaagt ataagtggaa attaactaaa ccagctgaat acataagtga tgaattaaca
    17221 tcaaaattaa agttaacgcc aatcgtaaaa aaaatattag aaagtaaatc gattattgat
    17281 gaacaagcaa ttgaatccat catttcagat acagatatca atcatgacgc attgcaattg
    17341 agcgatatga ctaagactat tgaaagaatt aaaagagcga tagcgaatga tgagaaaatc
    17401 ctagtttatg gtgattacga tgccgacggt gttacctcaa ctacaatttt agtgacaaca
    17461 ttacaacttt taggtgcaca agtaggatgg catattccta accgctttac cgaaggatat
    17521 ggaccaaacg agctagcatt tagaaatgct catgatgaag gcataacatt aatcataact
    17581 gttgataatg gcatacaagg tcataatgaa attaaaatgg ttcaagattt aggagttgat
    17641 gtcattgtaa ctgatcatca tgaaattggt agtacattac cagaagcata tgcaatcgta
    17701 catccaatgc atccatcgtt taattatccg ttccaacaat tgtgtggtgc aggtgttgcg
    17761 tacaaattag cacaagcatt gattgaaaat gtacctgatt attttaaagc attagttgcg
    17821 ataggtacca ttgcggattt agtatcatta actgatgaaa atcgatcatt agtgaaacaa
    17881 ggattaaaag tcttaaatga tcaatgtccg acctctgtta aagcattgct aaaagaagcg
    17941 ggatacaatg acaatattga tgaagaaaca attggtttta ttattggacc tagacttaat
    18001 gctgtaggtc gtttagatga cgcatcatta gcttgcgaat tgttgatgac tgacgacgaa
    18061 gaagaagctg catttttagc agaacaagtt gaacaattta accgcgaaag aaaagatatt
    18121 gtagcaacta tcacagaaga agcaatggca atggcagaaa tgaaagttaa aaatggagat
    18181 ttatttttac ttttagcaaa agaaaattgg catgaaggtg tcttaggtat tgttgcttcg
    18241 aaaattgtcg aaacatttgc tttgccaaca ttaattttaa atattgatag ggagcaaaat
    18301 catgcaaaag gttccgccag atcaattgat caagtgtcga tgtttgaaat actatcagca
    18361 caccaagagt taatcgctaa atttggcggc catcatatgg ctgcaggtat gacgatggat
    18421 attgaaaata tagaatcgtt agctgaaggt ttaaataaat ggatgaaaga attatctaaa
    18481 acaacttcgt tagatcccgt aaagccagta gatgtattat taacagaaaa tgatataacg
    18541 attaaaaata ttcgtgatat gaatcgttta agacccttcg gaacagattt ttctagacct
    18601 atttttgaaa tggatgattt atcagtttct tcagttaagg cgataggtca acaaaaaaat
    18661 catctgaaat tgacacttgg tgaaagtaat atcgccgcgc tattttggca aaatggacac
    18721 ttagaacctg aattacaaga tgaacagcca attaatatat taggatctgt tcaaatcaac
    18781 gaatggaatg gtaatcaatc accgcaatta attattcaag atattgcgat gaacgaacag
    18841 caaatattag attatagaag taagcgaaaa agtttacctt ttacagaaaa tgatgaaaat
    18901 attgtcgtgc ttattcatcc taaaagtgat aaagtaaatg cgaatgaata ttattatggt
    18961 gaagaaatta aacaacaaac tgataaagta gtattaagag atttacctac gtcaatggaa
    19021 gatttgtcta attccctgca acaactgcaa ttttctcaac tttatatagt tttgcaacat
    19081 aatcattcga tttacttcga tggtatacct aatatggatg tttttaaaaa gtgttataaa
    19141 gcattaataa ctaaacaaga aacaaatatc cagaaagagg gtatgttatt gtgtcaacat
    19201 ttaagtgtga aaccagatac acttaaattc atgttgaaag ttttcttaga cttaaaattt
    19261 gtaacacaag aagatggttt aattcgaatc aatcaacaac ctgataaaag atcgattgat
    19321 tccagcaaag tatatcaatt aagacaacaa cgtatggatg ttgaaaagca attattatat
    19381 caagattttt cagaaataaa aaattggata aagtcacaat tgtcgtgagc aatttaggag
    19441 gaaatattaa tggatttaaa gcaatacgta tcagaagttc aagattggcc gaaaccaggt
    19501 gttagtttca aggatattac tacaattatg gataatggtg aagcatatgg ctatgcaaca
    19561 gataaaattg tagaatacgc aaaagacaga gatgttgata tcgttgtagg acctgaagcg
    19621 cgtggcttta tcattggctg tcctgtagct tattcaatgg ggattggctt tgcacctgtt
    19681 agaaaagaag ggaaattacc tcgtgaagtc attcgttatg agtatgacct agaatatggt
    19741 acaaatgttt taacaatgca caaagatgca attaaaccag gtcaacgtgt gttaattaca
    19801 gatgatttat tagctactgg tggtacgatt gaagcagcaa taaaattagt cgaaaaatta
    19861 ggtggtatcg tagtaggtat tgcatttata attgaattga aatatttaaa tggtattgaa
    19921 aaaattaaag attacgatgt tatgagttta at
//
LOCUS       DATTQF010000032        19863 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.032, whole genome shotgun sequence.
ACCESSION   DATTQF010000032 DATTQF010000000
VERSION     DATTQF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.032"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..465
                     /gene="traP"
                     /locus_tag="VB241_001055"
     CDS             <1..465
                     /gene="traP"
                     /locus_tag="VB241_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HEP1244358.1"
                     /translation="LNQIKINNPSHHLFQFSTADSSVIFEETEENTVLKSPSIYEVIK
                     EIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKSYRLLRPVKGTTYK
                     IYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFERYLYPIKE"
     gene            complement(589..1812)
                     /locus_tag="VB241_001056"
     CDS             complement(589..1812)
                     /locus_tag="VB241_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244359.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYITFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGNKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLIVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEDKVIKGYEQFLYRLMFVICTVFALTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(1805..2545)
                     /locus_tag="VB241_001057"
     CDS             complement(1805..2545)
                     /locus_tag="VB241_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244360.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2679..3101
                     /locus_tag="VB241_001058"
     CDS             2679..3101
                     /locus_tag="VB241_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP1244361.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            3243..3608
                     /locus_tag="VB241_001059"
     CDS             3243..3608
                     /locus_tag="VB241_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP1244362.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            4341..4898
                     /locus_tag="VB241_001060"
     CDS             4341..4898
                     /locus_tag="VB241_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEP1244363.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            5103..6065
                     /locus_tag="VB241_001061"
     CDS             5103..6065
                     /locus_tag="VB241_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP1244364.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFKKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGTQGGQSGMSQ"
     gene            complement(6186..7127)
                     /gene="yhaM"
                     /locus_tag="VB241_001062"
     CDS             complement(6186..7127)
                     /gene="yhaM"
                     /locus_tag="VB241_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HEP1244365.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(7124..10060)
                     /locus_tag="VB241_001063"
     CDS             complement(7124..10060)
                     /locus_tag="VB241_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1244366.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTVFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVYYSEAITDEIEVIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            complement(10050..11246)
                     /locus_tag="VB241_001064"
     CDS             complement(10050..11246)
                     /locus_tag="VB241_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEP1244367.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            complement(12134..12478)
                     /locus_tag="VB241_001065"
     CDS             complement(12134..12478)
                     /locus_tag="VB241_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP1244368.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            complement(12547..13671)
                     /locus_tag="VB241_001066"
     CDS             complement(12547..13671)
                     /locus_tag="VB241_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEP1244369.1"
                     /translation="MNALFIIIFMIVVGAIIGGVTNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIASQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARAIVTSLITNEYQTFKDKSLNKLLDTSQFNEIAENLSMYVTTYASKQ
                     ANKPVVTLMPQFVDYLESQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            complement(13850..14314)
                     /locus_tag="VB241_001067"
     CDS             complement(13850..14314)
                     /locus_tag="VB241_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1244370.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            complement(14672..15295)
                     /locus_tag="VB241_001068"
     CDS             complement(14672..15295)
                     /locus_tag="VB241_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1244371.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            complement(15317..16429)
                     /locus_tag="VB241_001069"
     CDS             complement(15317..16429)
                     /locus_tag="VB241_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEP1244372.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            16592..17413
                     /locus_tag="VB241_001070"
     CDS             16592..17413
                     /locus_tag="VB241_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1244373.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            complement(17869..19254)
                     /gene="fumC"
                     /locus_tag="VB241_001071"
     CDS             complement(17869..19254)
                     /gene="fumC"
                     /locus_tag="VB241_001071"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEP1244374.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHHSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAQYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(19450..19845)
                     /locus_tag="VB241_001072"
     CDS             complement(19450..19845)
                     /locus_tag="VB241_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244375.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPIIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
BASE COUNT         6470 a         3350 c         2829 g         7214 t
ORIGIN      
        1 ttaaatcaga taaaaatcaa taatccatct catcatttat tccaattttc aactgctgat
       61 tcatcggtga tttttgaaga gactgaagaa aatactgtac tgaaatcacc ttcaatatat
      121 gaagttatta aagaaatagg cgcattcaat gaagatcatt tttattgcgc aatttttatt
      181 ccgtcaactg aagaccatgt ttatcaactt gaaaagaaat taatcagtgt tgatgacaat
      241 ttcaaaaact ttggtggctt taaaagttat cgattgctaa gacctgttaa aggtacaaca
      301 tacaaaattt actttggctt tgctgatcga caaacttatg aagactttaa aaattctgat
      361 gcttttaaag atcatttttc aaaagaagca ttaagtcatt actttggttc aagtggacaa
      421 cattcaagtt attttgaaag atatttatac ccaataaaag aatagattcg aagaaaactt
      481 ccattggtta tgctgttaac gtatagatgc taatgtaatc tgaacgccgt atcatttggc
      541 tttcaacatt acatgcacct ttacaacata catgccaatg gaagtttttt agtctcgtaa
      601 taatgtttcc tgatatttta actttttaat aatacttcta atcgttaata aacctacaat
      661 atagaaaatg agtacaacat agaataacgt catatgtttt ataataaatg tcaatgcaaa
      721 cacagtacaa ataacaaaca ttaatctata taaaaactgt tcataccctt tgattacctt
      781 atcttcaggt acaggccaca cttgaggcca taagccatat gcttgttgtg aataaaattg
      841 tgccatttgt aacaatataa tatatacaaa taaacaccca ataattgctg tcactaatgg
      901 atatgataac caaactataa gtaaaactgc aataattact aacctaaaga taatattaaa
      961 tgcgtctctc cctcttataa agcttctaat aaataagaat aaatacatcg cattagagtt
     1021 aaatttatta ccctttggaa ctggtaaaag tatatctaga taacttcttc tgactgcaga
     1081 ttctttcaaa tgttttacat cggtaaacat attaacaaat ttataataat tcatatgatg
     1141 tcgatgttcg atagcaatca ttttctccca tggatacaaa aagccaggtt tatatttttt
     1201 aactaaaaat tctattaaca caggcaaagc aaccatcaca aatgtgatgt accattttgg
     1261 agctaatagt aagtaatatg ttagagcaaa gatgatgaat gatattaaat taacttgcca
     1321 tgttttaagt cccgattgat accattgcca tcttaagcgt aaaccaacat atggaaaaat
     1381 taatgcactg actccaaaac aaatataaaa tgccacatta tgttgattaa tattgtaaaa
     1441 caacgggaac attacaataa caataatgag ttggattaaa atgcgcgcaa agtagctata
     1501 taaaatcgca tgacgcataa attgagacat gtgtttttca aatggtaata aaaagatttt
     1561 atccgcttct tttaggagtg gtcgcattgg aaaaatggat gtcaacgcaa caatcactgc
     1621 tgctattaat gaaaaattga tattcgttgg aatatgtttt aaccattcac catatccaaa
     1681 aataaatgca cccagcaaaa taagtaaaaa gaccatgaaa tgaccattaa atataaactt
     1741 attataataa tttttctctt tacgaagggc atgtaatctt ttattaaata atgtggtcgc
     1801 ttggttacgc atgtacatct ccaccttgcg tcacatgaat atatatatcg tctaacgttt
     1861 gattatgtaa gccagtttgt tgtctcaatg cttctaaatc tccaaatgca acgacttcac
     1921 cttcgtctag tatgataaaa cgatcacaat aacgttcagc tgttgctaaa atatgtgtac
     1981 tcattagaac ggttctacct tcgtttttct tttcaaccat taaatctaac atggattgaa
     2041 ttcctaatgg atctaggcca aggaatggtt cgtctataat atacaattcg ggattaacga
     2101 taaacgcaca aataatcatg actttttgtt tcatcccctt agaaaaatga ctcggaaaaa
     2161 ctttcaattc attttctaaa cggaatgtct ttaataatgg cattgctcga ttcatcgctt
     2221 catcacgatc aatatcatat gccattgctg tcatctcaat gtgttcctct aatgtgagtt
     2281 cttcgtatat aaccggtgat tccggaatat aagataactt tcttctataa gcctctatgt
     2341 catcattaat gttgatatct gaaattgata gagatccttc cataggtgta agcaatccta
     2401 gcatatgttt aatcgttgta ctcttaccag cgccattaag gccaataagt ccaacgattt
     2461 cgcctttgtt taattcaaaa tttatatctt taattacagg gcgttttcca tatccacctg
     2521 taagctgttc tactttaact gtcataaggc acctccatga cttatattgt accaaaaatt
     2581 ataaaatgct catattaaat acacatatcc taatatcgaa tttttagcga caatgttata
     2641 atgaatggta atacaagttg aaaaggagtg tagtcatcat gtcagaaaca attttcggca
     2701 aaattttaac tggagaaatt cctagcttta aagtatatga agacgattat gtctatgcct
     2761 ttttagatat atcacaagtt actaaaggac atacgttatt aattcctaaa aaagcttctg
     2821 ctaatatctt tgaaactgat gaagaaacaa tgaaacatat cggcgcagca ttacctaaag
     2881 tagcaaatgc tattaagcgt gcatttaatc ctgatggttt aaacattatt caaaataatg
     2941 gtgagtttgc agatcaatcg gtatttcata ttcatttcca cttaattcct cgatatgaaa
     3001 atgatattga tggatttggt tataagtggg aaacacatga agatatttta gataacgatg
     3061 caaaacaaca aattgctgaa caaattcaag cacaatttta aatgtatgct taatctaagc
     3121 tcgaacgggt ataatatgat taatattata acaattgcgt ttgaagtgat aacatcaagg
     3181 ttagcaattt taaacaaaat gagttattaa gataacagat gttaaaagtg aggagaatat
     3241 aaatgaaagc atcacgcatt ctattcggta tcggtgttgg cgtagcagct ggttttgtag
     3301 ttgcacttca aggacgtgac gacaaaagtg tcaagaacaa cacgattgat cgtactgccc
     3361 ctactggttc aaaatcagaa ctacaacgtg aatttgaaac gattaaacaa agttttaatg
     3421 acattttaaa ctatggtgtt caaattaaaa acgaaagtgc ggaatttggt agttcaatcg
     3481 gtggtgaaat taagtcatta cttggaaact tcaaatctga catcaatcct aatattgaac
     3541 gtttacagtc acacatcgaa aatttacaaa atcgtggcga ggatattgga aacgaaattt
     3601 ctaagtagca cgtttacgtt ctcgatcaca actattttta ttagtaacag catatttatt
     3661 ttttaaaatt aaatgccaaa taaacgagat gacattagaa attagatatt tcttgtcatc
     3721 tcttttttat ggctcgaatg aatgtttgat taccaattac agtaagacgt tgtttgtagt
     3781 gattttccct taaatcatat ttatgaattg attgaaaata ttgcttagga ttcattgtgt
     3841 tatccttgca ctttgattac gctttactta aatcattatc gacaaacaac atacttatat
     3901 tttcactgag ccgaacctta tatgcacatt acatatacct tacttccaca aattattaat
     3961 ctggtattta ttataattac atatcactat atttttagca tttgtatgac ttagttagtt
     4021 aaaagatgct tttgcatatg ccctgccata tccatttttt atattattca agttttcaaa
     4081 tactcatgtc acacgcgcct ttttcataag tgatgcttta ttagcaaaaa tatgtgttcg
     4141 cagaaatttg ttctgcattc tacttctacg ctagtcaatc agccaatttt accaatccca
     4201 ctttcgcgtt tcaaatcaaa caatacgtcg cccctttctt catatataac aaatcttcta
     4261 acatgatatg ttactattga attactgaac ctgagttagt tataatctaa cttatattga
     4321 aaagagatga ggcgtaagat atgtttttat gtaaaagaca aattgatatc aatgcacgat
     4381 ttggtttgcc tagaattgca tttatgagtg cagttgcaac catcattatg tttttagtta
     4441 gttatgaagt aatgtatttt ttatctaata cgccgttatc agatagacat tttctcatct
     4501 ttttattact tgtatttatg acgtatccat tacataaaag tatacattta ttatttttct
     4561 taccatatag aaaatcgttt aaagttcata agttgactaa aagaaaatgg cttatattct
     4621 ataataccta cgtcaatcaa cctgtacaca aattttattt ttgcattaac ttaatattgc
     4681 cgttaattat cttatctgca atgttcgttt atctaacaat ttcattcccg caatatggac
     4741 attattttat gttcttattg gcattgaatt tcggtatttc cattacagat ttattatatt
     4801 taaaaataat tatattttct aattatggac aatatataga agaacatagt acaggtatta
     4861 atattttgaa aaaaattaaa aatccatatc atttataaca aaataattat agcaaggtgt
     4921 tattatttgt tttcaggcta tgtaatagct tacaatcaaa tgtatataga ccttgttttt
     4981 ttattttcat caatttctac ccctaaacct aatgctctag tctgatgtca tgggttattg
     5041 attggtgata atataaaact atgttatatt cacgatgatt aacttacaaa ggagtttcaa
     5101 ctatgaagat gataaacaaa ttaatcgttc cggtaacagc tagtgcttta ttattaggcg
     5161 cttgtggcgc tagtgccaca gattctaaag aaaatacatt aatttcttct aaagctggag
     5221 atgtaacagt tgcagataca atgaaaaaaa tcggtaaaga tcaaattgca aatgcatcgt
     5281 ttactgaaat gttaaataaa attttagctg ataaatataa aaataaagtt aatgataaga
     5341 agattgacga acaaattgaa aaaatgcaaa agcaatacgg cggtaaagat aaatttaaaa
     5401 aggcccttca acagcaaggt ttaacagccg ataaatataa agaaaattta cgtactgctg
     5461 cttatcataa agaattacta tcagataaaa ttaaaatctc tgattctgaa attaaagaag
     5521 acagcaagaa agcttcacac attttaatta aagttaaatc taagaaaagc gacaaagaag
     5581 gcttagatga taaagaagcg aaacaaaaag ctgaagaaat tcaaaaagaa gtttcaaaag
     5641 atccaagtaa atttggtgaa atcgctaaaa aagaatcaat ggatactggt tcagctaaaa
     5701 aagatggcga attaggttat gttcttaaag gacaaactga taaagatttt gaaaaagcac
     5761 tatttaaact taaagatggt gaagtatcag acgttgttaa atcaagcttt ggatatcata
     5821 ttattaaagc tgataaacca acagacttta acagtgaaaa acaaagcctg aaagaaaaat
     5881 tagtcgatca gaaagtacaa aaaaatccaa aattattaac tgatgcatac aaagatctat
     5941 taaaagaata cgatgttgac tttaaagatc gtgatattaa atcagttgtc gaagataaaa
     6001 tcttaaaccc tgaaaaactt aaacaaggtg gcacacaagg cggacaatcc ggcatgagcc
     6061 aataacacaa aaccgagcga ccgtggttta aaaatcatac cacggccgct cggttttttc
     6121 gcattaaaaa tcggacagat gagctcatgt ttcagtatac tcatctgtcc gatatctttt
     6181 aattcttaat cgagtgattc aggattgtag aatctacgat tttcaagacc aaatatttta
     6241 tctgtaaact gacccttgtc agttttttta taagcctttt caaacatatt cattctagca
     6301 tcgatattat cgatatagca taaaatttct gcttctttta agtatggcag ctttggcgaa
     6361 ccatactcta acttaccatg atgagataaa atcatatgtc ttaacaacat gatttcttct
     6421 ccttcaatgt tcaattcacg agctgcttca actacttcat cactcgcaat cgagatgtgt
     6481 cctaataaat taccttcgac tgtatacgac gtcgcaacag gaccactcaa ttctctaact
     6541 ttaccaatat catgcaaaat aataccacta tataacaaac ttttgtttaa caatggataa
     6601 atgtcacaaa ttgattttgc aatacgtaac atcgttaata catgatagct taagccactc
     6661 gcaaagttat gatgatgaga actagcagct ggatatgtgt aaaatcgttc ttgatatttt
     6721 ttcaataaat gacgtgtgat acgttgtaaa ttagcatttt caatatctag caaataatga
     6781 gaaatctctt cttgtatttc tgccggtgat aaaggtgcac catctacaaa ttgttctgtt
     6841 tttaattgat cttcagttgt cgctagtcta atttggttga ctttcatctg tttatttccg
     6901 cgatagttta tgatgtcacc tttaacatgt acaatttctt caggcttgat tgttgccata
     6961 tcattttttg tagccgtcca aaatttcgct tcaatttcac cacttttatc ttgcaaatgt
     7021 aatgtcatat aatctttacc ttgtgctgtt acaccctgtg tagctttatg cactaagaaa
     7081 aagtgatcaa ctgaatctcc gggatttaga ttctctatat ttctcatcgt ttcccgcctt
     7141 cctctatttt gtttaatgta atcacttctt ttgatggaac aatattatct tttacacatg
     7201 taaagtatag tacttgatag tgttctgata atgatcgtaa ataattcaac attttttcag
     7261 tacgtttttt atcaaaatga acaaatgcat catcaacaat taatgggaac ggataatatg
     7321 gtcgtagtac cttaattaaa ctgatacgta aagctacata aagtaattct tttgtagatt
     7381 gacttagttc aacaggatca tataattgac cattaacatg tttaaccgta attgaatctt
     7441 cattatagtt aatcatcgta tatctgccat ctgttaaatg cttcaatatt tctaccgctt
     7501 cattaataac ttgaggcaaa cgtttatctt taatttgttt aatgtgttca tcaactaaac
     7561 tttgtaaata acttaaactt gcccaatctt ttgcgatatc attaagttga tttttaagac
     7621 tgtgatattc atgtcttaaa ttagcaagcg ttgtatcagt ttccatgtga ttgatttgtg
     7681 cacttaaatc actgacttgt gcttgcattt caagatattg ctcattatat tcgtcaactt
     7741 gagtagccaa taaatgatct tcttcttcaa gttgtgctgt tgttttttca cttaaactag
     7801 aacttaattc ataagaatag ttttggttct caagatattt tgttaaatca ttaaaacgac
     7861 tcaaattact agtgtatgtt tggtaatctt catgatgttg gtaaaaatct tcttcagtac
     7921 caacattgat aaaatcgaat agtgctgtaa tttctttatt attttcttct aattgagcat
     7981 ttaaatgatt taattcattt gtaacaagtt tggtattttc agcattaata cgccattttt
     8041 cattcgtgtc ttcagctgat ttcaaccatt gttgcacatc gtggaataaa gataatttgt
     8101 tgaaataaac aaattgtgat tttgttacag cttcagcatg attgtagaat gtatctaatt
     8161 cttgaaccaa ttgctggcgt tgttgattta aatcactgat atgttgatct aatgctttaa
     8221 tattcgccat tgtagaaata ctatcaacaa ttaaatcatt tgaaatttta gatgataagt
     8281 ataattcatc cttaacgttt tcaactgtcg attgtaattc atcatgacgc cctttcgcat
     8341 catttaaacg accttcaata tactgacgtt tctcttctaa aatatcttta tttttcaaag
     8401 cttgttgcca gtgatcacga atgcgatatt gctcatcaag atcaaaatct aagtcatagt
     8461 tttcatctaa aatggctagt tgtgctttaa ttacttcgat ttcatctgtg atggcctcgc
     8521 tataatatac ttcttttgat ttagacatga tgataccgat aacaaatact aaagttaata
     8581 ctgcgaaaat aataccaaac aacatgttgt ttgaaataaa tgagaagaca gttaaaccaa
     8641 tacctactaa tgttaaaaga ataaacgttg ttcgtaacaa tttttgacgt ttttgttttt
     8701 cttgttgctc aatttcgaaa cgttctttca acttgctata caagttctct ttttcgttta
     8761 attcaatgac ttgttgtgag tattcttttt tcttttcaaa agtctcttca ggaactattt
     8821 tttcttcaac agaatctagt tcgctatgaa ccgcattatc ttcgatttta ttttcttcta
     8881 aactacgttc taattgctta atgtatgccg cttgttcttg tttattcttg atttgctcac
     8941 tgacataact tttcattgcc tcagagctat ctacgtcatg atgcgtttca gaccaaccaa
     9001 tatttgcttg caattcatct ttatcacgtt gtttattcgc aatatccttt tcgattgcag
     9061 taagttcaaa ttctttattt ttaatttcat tttcttgttg attcaaacta atgaaggcgt
     9121 caatatcaga ttgtttaact ggctctaatt gagtcgcttc ttctttaagt tgagctaaac
     9181 gctcatttct taaaccaata tctctttcta acgattgctt atgcgctcgt gctttttcgt
     9241 aacgatctac acctttttct gggaatgtga ttggctcaat atttaactgt tgttctagag
     9301 acttccattc ttgtgaatga tcatgtaaag caacctcttt ttgtttttct tcatgcattt
     9361 ttgataattg atttaaatta tgctttaaat tctctaatcg acgtgatgat ttatctcgat
     9421 catctactaa acgatgatat gtttctagtt tagcttcttc ttcacgaatt tgactttcta
     9481 gttgttttaa ttgctcaatt tgttgattaa taatcggatt tttacctgat tttttatata
     9541 attcatcttt tttacgatta atcacttcgc gcattgacgt gaattcagtt gatcctaaag
     9601 cccctgcttg taataaataa tcttgcaatt gtttttcatt tagatttcta tgaatgtctt
     9661 gaagccctag tacatcaaat gaaaagatac cttgatatgt ctttttagaa atataattaa
     9721 gtttcttttg taaccaagca tcatcacgca cagcaccatt aggtaaatat actttcacat
     9781 caccttgagc actgccttta attcgttcaa cttcaatctc taagccatca tcaagaataa
     9841 gtactaattt accaccgtat tggttaccta gacgtggttc tagtcttggc tctttagact
     9901 ttttagttgg aaaaccaaat aatatcgaat ggatgaatgc ttgaatcgtc gatttacccg
     9961 cttcattttc accaaaaatt tcagtgaagt ttttattaaa ttcaatttta cgttgaacaa
    10021 attgaccgta accataaatt tcaagtgatt taattatcat tttgttcacc tctcatttca
    10081 gcttttaata tttcttcagc acgattaact aatgctgtat ggtcgaatgt tccataatcg
    10141 tctaggaact tagatgccct tggatttaaa tataagtctg acatcgcttt atcaaaaaca
    10201 gtttgatcga ctaataattc cgctgaaaat tcattaacta aaggtgactc atcattttgt
    10261 gcatattgta tttttaactc atcaatatat acaaattgat tttcgttttc ttcataatct
    10321 gtaatcattt cttcaacttg taataaatct tgaggtgaaa ttaatgtctc actattaata
    10381 acaagcgtta aacgataaaa ggctttccct tcttctctca cttgttcttt aaagttttga
    10441 atgacctcgt ataaaccttg cttagatgtc ttatccgttt caatagttgc ttcttcaaat
    10501 ctaatatact gtgtaggata aaatttagtt tttaatttta agtggtcacc ctcgattaat
    10561 aagcaacctt tttcaccttg ctcattaaaa tgtctacctt gaatattacc tgaatagtta
    10621 attacaggca tatcacttaa ttgttgacgt tcatgtatat gacctaaagc ccaataatga
    10681 tacaatttac tgtttaaatc ttctaaaatg aactcggtat atctttcgtt aactgaagat
    10741 ttactgtacg taccatgcaa gacaccaata tgtatgcctt tttggccttg acttgatgga
    10801 tattcatcaa tcttgttctc ataacttgct ctattttcat aactaaatcc gtgaatataa
    10861 attgtttcac cagatttagt aattgcttca tacgtctcaa ctttatttga aaatacagaa
    10921 acattatctg gccagtttga tgaaatcttt gatgataatg ggtcgtgatt accatgacaa
    10981 acataaacaa agatttgttc attttgtaaa cgttcaaatt gctgttttaa gaaaatttca
    11041 gcacgtaatg ttctgttttc actatcaaac aaatcacccg cgataattac aaaatcaaca
    11101 tcttgttgta atgcaatatc tacaatgttt ttaaagcttt cataagcact tttttgaaca
    11161 tcctcaaaaa ttttagggct tatatgactc ttagatttga aaggactatc taaatgtaaa
    11221 tccgaacaat gaataaattt aaccatacat ccatatctcc ttaattaaag gttctaacat
    11281 caattttgca aaatccaact atacaaagca ttctaatcac tatattcata cttataggtg
    11341 tagtgattca ttagctgatt tattaaaacc agtcgccatt tttattttta tttcgttaaa
    11401 aaattattat ctacacaata ataaagtgct ttagtcaaat cgtgatgttt tatttattaa
    11461 caagtttatt atttcttcat tttaccataa tacgcttcat atcgtcgatg aacatatgaa
    11521 attgagacac atttataaca gtttaaaatt ttatttgtag cgtgcgttga tttttttcaa
    11581 aaatttcagt cgaaaatgta atttccgtaa ttgttaacac cgctagtgaa ccaactgata
    11641 cattaaagat ttacatgatt actctactag atttgcttgg cagtaatgac caattagaag
    11701 ctaaatgtca aaactatcat tgcacttaat acgccaaaca ttttcaagcc gttcttaatg
    11761 agtcattaaa agtgaaacac ttttccatca gtccgataca tgaccgaact aatagtatta
    11821 tgctcaaaat tttacactcc aaaatgcaaa aaggaagtta ggacacgttt actagcctaa
    11881 cttccgttat caagctcatc cgcccgtttt ggcatagagc caatattgtt tgattgccca
    11941 gcttcagtcg tccgttctga gctaaagtta agacatgttt aataacctaa cttccgatat
    12001 tgaacgcatc tgcccaattt gacagggggc ctccaacttc cgttatcaaa actaaaaaaa
    12061 ctacccacaa agtctaggac tctgtcggca gtttattcct ctatttaaag gattgaaaaa
    12121 tattatttta atattagtca gcgtaaattt cgtctaatgg tttaacgata atttggttga
    12181 tttcttggaa tacttgactc attttttgtt cagcattcat taatgcagag atgttttcat
    12241 ctttttcaat tgcttgcgct tgttcttgcg ctttttgtaa atcttcttca gcaatttctt
    12301 caccttgcat ttgtttttgt tggaagttaa tttgagtttc acggaactcg tcgaataact
    12361 ttttagattc ttcgttagct tttacattag cgaatgcttc tttgattgct ttgtattctt
    12421 cgctttctct taaagcttgt tctaattgat ttgcataatc atataaattt actgccatgg
    12481 ttatagcact cctttgttgt gatgtttatt aaaacgttcg atattacttt accacatctc
    12541 gcttttttag acaaagattg caaccaaacc ttggaaaaat ccaataatac cacctaaaat
    12601 gaaacctaat gacataatca actttagttc tttgttagca atttctataa ttaatttttc
    12661 aatataatct aagtcaaacg tattaatttg ttcttcaatt aatcctcgta aatccacttt
    12721 tttcataatt gtagataagt gaatcgataa ttgttcaatg attaaatttg ctaatttgct
    12781 agagagttga ctttctaaat aatcaacaaa ttgaggcatt aacgtaacca ctggcttatt
    12841 cgcttgtttg cttgcatatg ttgttacata cattgataga ttttcagcga tttcattaaa
    12901 ctgcgacgta tctaacagtt tattcaatga tttatcttta aaagtttgat attcattggt
    12961 aattaacgat gtcacaattg ctcttgcttt aggatgagat gttagacgta taagttcttg
    13021 ttgaattcga tctgcaatgc tctcttttgt cataaacatt tgcaacatac caattaactt
    13081 acctttctca ttgaaaaaag tatccaacat atcattaata tcttgtgtgc cttttgcaga
    13141 tgataaataa tttcttgcac gatcacataa taagtctgtt gcgttatcta catgctgatc
    13201 taaaaatgtt acaagttgat ttggcaataa agatgctatt gtttggtttt gatgctttgt
    13261 ataataatta ttcaattgtg atgcaatata ttgatttcca ttagtttgta atacttgttc
    13321 taaatcaata tcgatttgag aagtaatttg tttaattgac aattgatctt tcgtcaactt
    13381 ttgtaactgc tgttgaatca tagattctat tgcttgttgt gattgctcgc tttttaattt
    13441 ttcattaatt aaagtttctg taagcaaatg ctcttcaatc acttggccaa ttttagttgc
    13501 aatttcttcg cgtcttttcg gtattaaacc tggtgtaaat gggactctaa atttaaatat
    13561 atagtatggt ttaaaggggt gaaacagcat tctaattgca attacattag taacaccacc
    13621 aattatcgcc cctactacaa tcataaatat gataataaat agtgcattca taattttgac
    13681 tcctttatga caacatagtt attcattata ccactatttt tattttttaa ttcattaatc
    13741 atttcacttt ctgctcaatt tattattagt ttacatacaa aaagcgatgc ctcacctctc
    13801 gaaacaggtg tgttacatcg cttttattat ttattaaaag tatataggcc tagatatgta
    13861 ctaattcttc tttagcattt ctgttaaagt atgtttcagc ttctctcatt ttagaagtac
    13921 cgaaaattgg ttggttatct ggatttaata cacgataaat attgcttact ttgttacttt
    13981 gtaaaatgta cccattacga gtctcaatgt tattccaata aatatcactt gttggtgcat
    14041 ggtttggata tgcacagtga tttcttgaaa ttgtttgaac gatttctaat ggatcacagc
    14101 caaatgtact gtttaatact tcagagtctc tgaataatgc acaaattgaa atacaagttg
    14161 tccagtttgg taatactctc tctttttcga tttgtactaa agtcttttta gaaagtccaa
    14221 ttgtttgcgc catagtatct tgcgtataac cagcctctat acgaaccatt ttaaattttg
    14281 tttgaattaa atctgtaaaa ctctgtctat ccattctgtt atctaccttt ctgtttgggg
    14341 aattttatcc ggacacaaga aattgcaata atacacattt cttgaaacac agattacatc
    14401 ttaatatatt tttaataaaa tgaaaagagt caatttcaca tttgtattaa attttgatca
    14461 agtcaacaaa agttaagttt acttcataat gaaacaaaag taaacgaagt taaattttat
    14521 gcattatgtc tacatttgat aaaacttaca cttttattat gatatcaaat ttacaaagaa
    14581 aactgtataa taaatgctat cgctgtaaaa aaaatctaaa taaatcgatt aaaaattgtc
    14641 gattttttga cgaagctaca tcaacgcaac tttaaatcaa cttattttcc attgcataaa
    14701 ttgctgcttg tgtacgatcg ctaacttgta atttactaaa tatatgactg acatgtgttt
    14761 taattgtttt ttcagataca aataaagttt ctgctatctc tttatttgtt ttacctttaa
    14821 ccatttcacg taacacttca atttctctct ttgacaactt attcgtgtag tgtggttttt
    14881 ggctaactgt ttcgaataca tcttgtgcct taggatgtat cattttttca ccgttcataa
    14941 ctcgtctaat agtttcaatt aattgctgag gctcaacgtc tttcatttca taaccatcag
    15001 cacctttatt gattgctgaa attacatgtt catcatcaac ataacttgtt aataccaaaa
    15061 ctttaatatc cggataatgt gccttaatat attccgtaat ttcaatacca ttcatgccag
    15121 gcatcactaa atctaatagc acaatatcag gatgctcatg ctcttttaaa tattctaaaa
    15181 atgtttctcc atctgcaaag tcttgtaaaa cttctatgtt ttcaatcgtg gataataaaa
    15241 atcgcaatcc ttgtcgcaca atataatggt catctactaa tattactttg ttcatgggtt
    15301 atctccttaa atcaagctat tttataggaa ttgtaaattg tatttgtgta ccctttgttg
    15361 gctgagaatg aaaggtcact ttacctctta ataatttaac tctttgtttt atgttattta
    15421 taccgtgtga tgaagctatc tgaacattat cgatctcaaa tccttgacca taatcaatca
    15481 cgtcaatata tagtatatcg ttcatttgtt ttaatgtaag atccatttta ttcgtatcag
    15541 catgtttctt aacattatta atacactctt gtaatgctct gtatatgttt tcttcgattt
    15601 cattagataa atcgattaaa ccttctacat ttacatttaa ttgtatatgc attaatttac
    15661 tatatgctgt caaagcatga attaagcctt gctcaagtcc aactggctta agttgccaaa
    15721 tcaatgcacg catttcatta acggcatttt gactcgtttc ctcaatcgtc ttgaaagctt
    15781 gtttagcaat ggattcgttt gacatgccat acgcagcatg tgctgttagt tttacagaaa
    15841 ataacatttg atttactgaa tcatgtaaat ctctagctaa acgattacgt tcattaattt
    15901 ttgctgcttc tttttcacgg tctgttaaat aaatacgttt gatggctgac cctaattgaa
    15961 atgcgacaga ctctagcaac tctaaatctt catcactata aatttcagta tttggtgacg
    16021 ctacatttaa aatgccgaat tgttcttgac ccgatttaag tggtaccgtt gcatgatgtg
    16081 taatattgtc attttggcta ggaaatgctt tagaggctaa gttaatacga gaacaattga
    16141 cgatattcga cgctttcatt agcctacgtt gattaaatgc tttcacacac caacaagacc
    16201 catctttaat atagtgacag tggtctgctg tcaaagattg tggtaaagcc acatgtgata
    16261 caagttcatg ttcacctacg ctattgatga aaaatatcca gcctgtcgtg aaattactgc
    16321 cctcaattaa atattttaac gcaccttggg tcatgctata catttcagtt tcttcattta
    16381 aaaattcggc aatctctttt aataaagcta gtcgcgtcct ttgttccatc aaatcgctcc
    16441 aattcatttt tacgtgtatt aactattata cattgagtta ttatattttt aaatcttaga
    16501 cgtgaacatg ataaaatggc cttgattact taatagttat atttcggaga actgatttgt
    16561 gatatgatat taaagactat aggaggattt tatgaaattt aaaataccag aaaactttaa
    16621 tgacttaagt ttacgagata ttttccaaca acttaaggta cctaaaaaag atttacatca
    16681 tttaaatatg tctaaagata ttactattaa tgataaacct gcgcgattaa tggataaagt
    16741 gcatactggc gacgatgtat ttgttccaac catcgatgaa aaaagtaatt atgttccaag
    16801 ttatcgttat gcacaaatta aatacgaaga tgatgatatg gcaatcgtaa tgaaacctaa
    16861 aggtgttaag acccacccta atgatttaaa agaaagcaat actttaatga atcatgtgat
    16921 ttacacggtt gatagcgatt atgtcgaacc aattcatcga ctggaccagg aaacagtagg
    16981 attattaatt gttgctaaaa atcctttaat gaaaaaaatt cttgatcgca tgttagaaga
    17041 caatgatatt acgcggatat acaaagcaaa tgttaaggca cttttacctt taaaaccaca
    17101 aacgattgat atgccaattg gtaaagataa attccattcg aataaacgac gtgtgtctcc
    17161 tactggacag cgtgcaatta cacacatttt aacttcaaaa atgataaaag aaactgtgtg
    17221 ccaacttgaa atcaagttgg atactggacg tactcatcaa attcgtgtgc atttagctga
    17281 aattggtcac ccagttattg gtgatccttt atacggtgat tcaacgttaa gacaattaga
    17341 acttgaaagt tacaaaatag agtttgtgca tcccttgact aaggaagtca tttccgtttc
    17401 tttggatgac taatttgttt agtttggcat gatatttaaa tatgcaatac tgcattgtaa
    17461 ctaaatcaag tatcaactta aatcggatag atggaaaatt attaattttt tcagatgttc
    17521 ggttttttta tgttttttac gaccttagga ttttatattt tgaatgtatt tggatttata
    17581 gcatccttgg ataatgactt gtagtctttt tcaactgcgt tacgtgtatc taaggacaat
    17641 acatgacatc ataagatttt tatcacatat tgtttggtca atacatgtac aacaattcat
    17701 caaataaaaa ataggttcta taataaaatg gaatccatga aaagtttttc ctttcatggc
    17761 tctatatcaa atcggactta taagttcaat ttgacataga caaaaaaata aagcttgaac
    17821 actacatcta cctgtaatgc ccaaacttta ttttatatta atatttaatt aatgaggatc
    17881 taccatatct tctggtttaa tccatgcttc aaattgttct tctgtaacat atccagtttg
    17941 aattgcagat tcttttaaag ttaaaccttc tttatgggct ttcttagcaa tttgagcagc
    18001 tttttcataa ccaatatgtg gatttaatgc agtaactaac attaatgatt gatttaaata
    18061 attatcaata ttctcttcga ttggttcaat gcccactgca caattgttat taaatgtttc
    18121 cattccgtca gctaaaaggt aaattgattg tagtgtatta tgcataataa ctggtttata
    18181 aacattcaat tcaaagttac cttgtgaact tgcgaagcca acaactgtat cattacccat
    18241 tacttggact gcaaccattg ttaacatttc acattgtgta ggattaactt taccaggcat
    18301 aattgatgaa cctggttcat tttcagggat agaaatttct gccaaaccag ctcgtggccc
    18361 tgaagccaac catctcacat cattagcaat tttcattaag tctcctgcta gcgccttcaa
    18421 tgttccgtgt agttgtacta cttcatcatg tgctgttaat gcatggaatt tattttcaga
    18481 agatacaaat ggataacccg tattttcaga aatatactgt gccactttat caccaaactc
    18541 aggatgtgca ttaatacctg taccaacagc tgtaccaccg atggcaagat ttaaaatgtg
    18601 cttcttagat tcagataaca ttgtttcgca acggtcaagc atataacgcc agccactaat
    18661 ctcttgtcct agtttgatcg gcgttgcatc ttgtaaatgt gtacgaccaa tttttataat
    18721 tgaatcaaat ttatcttctt tttctttcaa agtatttctt aaaagtttta atgcaggttc
    18781 taattttgtt tcaacctctt gatatagtgc aacatgcata gcagttggga atgtatcatt
    18841 cgaactttgt gatttattta catcatcatt tgggtggata ctttcatcac tatgatgatc
    18901 ttttaaatac atattagcaa cataacttac tacttcgttc acattcatat tactttgtgt
    18961 accgcttcct gtttgccata caactagtgg gaagtgttca tctaattcac ctgataaaat
    19021 ttgatcacat gcgtatacaa tggcatcttt ctttgcctcg cttaattttc ctaaatcaaa
    19081 attagctaat gctgctgcac gctttagttg tgcaaaacca taaactactt cgattggcat
    19141 acgctcttta ccaactggga aattacgttt acttctttct gtttgagcac cccaatattt
    19201 atctgcaggt acttctattt ctccaaaagt atcatgttca attcttactg acattcaatt
    19261 tctcccctta tcactgttta tttaactgta gtatatcatt aaataattta attgagcaat
    19321 ttatgattaa aacgttttca taatttgaaa taaaaataca ctaatcgcac gtgcccaccc
    19381 tttattacag tgatacggtc atacgattag tgtgttatct atcattattt agttaatgtt
    19441 gaactaagtt taattacgat actttgtttt agtagcttca accgtagcaa tagctgtaag
    19501 tatatataat acagcactaa caattgtcgt atatggattt agagcaacaa gcgtacctaa
    19561 tactcctgtt aaactcgcat aaaacccatc aagtgtataa tacaatattc ctgttattaa
    19621 taaacaacaa attacactta ttacaatacc tgattgatta cttttaatga atgtttgcgc
    19681 attaacatca tcgattaatc cttttgataa attgagttgt aattttatta ctttgaaaat
    19741 aataggtaaa tataatgccc caattgccaa tggaaatgct ttaattgata ttaaacttat
    19801 aataactgtt gctatcaata attgaatcca gtattttcct aacataaata tataaatctc
    19861 ctc
//
LOCUS       DATTQF010000033        19750 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.033, whole genome shotgun sequence.
ACCESSION   DATTQF010000033 DATTQF010000000
VERSION     DATTQF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.033"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(106..435)
                     /locus_tag="VB241_001073"
     CDS             complement(106..435)
                     /locus_tag="VB241_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244376.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVFYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            484..1221
                     /gene="sdpB"
                     /locus_tag="VB241_001074"
     CDS             484..1221
                     /gene="sdpB"
                     /locus_tag="VB241_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HEP1244377.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYIKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1246..1938)
                     /locus_tag="VB241_001075"
     CDS             complement(1246..1938)
                     /locus_tag="VB241_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEP1244378.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2323..3120
                     /locus_tag="VB241_001076"
     CDS             2323..3120
                     /locus_tag="VB241_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEP1244379.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGFKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            3253..4200
                     /locus_tag="VB241_001077"
     CDS             3253..4200
                     /locus_tag="VB241_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP1244380.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            4802..7756
                     /gene="fdhF"
                     /locus_tag="VB241_001078"
     CDS             4802..7756
                     /gene="fdhF"
                     /locus_tag="VB241_001078"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEP1244381.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGQIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGDSKPDWKIFQAIANKLS
                     FDWNYKHPSEIMDEIARLTPLYAGVSYERLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMLKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRMEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
     gene            7756..8229
                     /locus_tag="VB241_001079"
     CDS             7756..8229
                     /locus_tag="VB241_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HEP1244382.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAVRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTSVRSLLGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            8523..8756
                     /locus_tag="VB241_001080"
     CDS             8523..8756
                     /locus_tag="VB241_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244383.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            8778..9554
                     /locus_tag="VB241_001081"
     CDS             8778..9554
                     /locus_tag="VB241_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HEP1244384.1"
                     /translation="MKKNFKLRISTLLLIVILVVFSVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAILGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            10163..11287
                     /locus_tag="VB241_001082"
     CDS             10163..11287
                     /locus_tag="VB241_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEP1244385.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRPLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(11379..12332)
                     /locus_tag="VB241_001083"
     CDS             complement(11379..12332)
                     /locus_tag="VB241_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEP1244386.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKD
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKNNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(12691..13182)
                     /locus_tag="VB241_001084"
     CDS             complement(12691..13182)
                     /locus_tag="VB241_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1244387.1"
                     /translation="MKKLVTATTLTAGIGTALVGHAQHADAAENYTNYNYNTTQTTTT
                     TTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHT
                     PSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNY
                     IHI"
     gene            complement(13419..13841)
                     /locus_tag="VB241_001085"
     CDS             complement(13419..13841)
                     /locus_tag="VB241_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEP1244388.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITFIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            14089..15171
                     /locus_tag="VB241_001086"
     CDS             14089..15171
                     /locus_tag="VB241_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEP1244389.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVESIGLDKLDLEKYLK"
     gene            15266..16666
                     /gene="nhaC"
                     /locus_tag="VB241_001087"
     CDS             15266..16666
                     /gene="nhaC"
                     /locus_tag="VB241_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEP1244390.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     AWIAKRVGLTWQDLETGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLSISPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIVGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISFSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            16840..17034
                     /locus_tag="VB241_001088"
     CDS             16840..17034
                     /locus_tag="VB241_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244391.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDAPKFGCVNGCMLFILY
                     YRVNRIGGNRSFEIFTNPIF"
     gene            complement(17103..17912)
                     /locus_tag="VB241_001089"
     CDS             complement(17103..17912)
                     /locus_tag="VB241_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1244392.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYSYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            18522..>19750
                     /locus_tag="VB241_001090"
     CDS             18522..>19750
                     /locus_tag="VB241_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1244393.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQPYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGDTPKLIRKRNSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTA"
BASE COUNT         6833 a         2739 c         3661 g         6517 t
ORIGIN      
        1 ttaatgcatc tttacaattt gaagaaactt tttaattgta taatttatgt aataaagtga
       61 attttaataa ataacttgtt taaatgacat tttatgaaaa ataggttaat gagcaaattt
      121 aattgcaaat aatgcgttag atagttctgt atgataaaaa ttaagatatt gaattttgtt
      181 accatagtcg acttgtcgga ggggacgttt attaagtagt gttataattt cataatagaa
      241 aacttgattt tcgtttatta tatatgatgt ttcaaagcct ttgaacttat tcaattcatt
      301 agtaactatt tggttaaatt tataaatatg cgtttgaatg ttccaatctg tcattaaagt
      361 attatattta atcgtatcaa tgatttgaat caaattagta atttgttgtc tcatatactt
      421 agtttcgttt atcatgtaga aggacctcca aaaatcaatt attaaacagg aaaggatata
      481 acagtggaaa atgaaaaaaa gaaatacacg tttaaagata tagcatggag agatttgctt
      541 ataattccaa ttataggtgt tatattttat gcactcacaa ttgcagctta cataggacca
      601 attttgtttg gttattatga aatcgatagt aatctgatat ttgtagcgac tatcacacaa
      661 gctttagcat atacgattgg gattggcgca ttttatttat ttcatttaaa agtgatgcca
      721 tcaaggcttc gagctggttt tgcgtatatc aaaaaatatt ggcttagatt gtcaataacg
      781 tatattattg ctctcatctt aatttataca tatgaatata tgacacagtt tctacctaaa
      841 catttgcaat atagtgaaac agccaatgaa cttgagctaa ataaaatgtt tgaagtgcct
      901 gcatttttac ctgtagcatt cttattaatt gtgattgtag gtccaatagt tgaagaaatc
      961 gtatttagac atattttaat tggagaatta ggtaagaagt ttaattttat agctatgagt
     1021 attgtttcgg tgttcttatt tgcatttatt catgtgactg atgcaaagtc gccgtttgag
     1081 tttggtccat atctcatact atccatcata ttagttttca cttatataaa atctggaaga
     1141 aacctaggtt caacaattgc attacatata gcaaataatt ttgtttcttt cgttatatca
     1201 gtaataagta tttatgcgta gataaaaccc cgtcgactcc atagtttagg aatcgatggg
     1261 gtttaaaaat tgatgatagt tatcaatgga tatgtctctg gtttgaatcg tcttaccgaa
     1321 tgaatacgaa atatctataa tattttcaat ttcgagttcg gcagttttgt cttgagcaat
     1381 gtcataaatg actgcatgcc aatgtccatg tttacacaaa aggccaataa atataacgtt
     1441 ttcggcttta atattatcat caaccctatg atctactaac atcacatttc gttctgtaca
     1501 ataaattaaa atgtcggaaa acaacattgg gagtgtcata tgttgtgtgt catcaaattt
     1561 aatataaaag tccatgcgct taagcaactt tcttaagcgt gtttgtggaa gacttaaaca
     1621 ttgttttata attgcgttga tttcgctttt atatggtaat gtcgagtacg actgactttc
     1681 attaagcact aaaaataagg ccgataattg tgtttcagat aagtttaatg caattttaga
     1741 ttgtgcttgt tgtatttggt aaccaccatt tttcccataa tgcgcataaa ttttaacacc
     1801 ttgattttct aaatcatcaa tatctcttaa aattgtgcgt ttggatacgt tgcaatattt
     1861 agctaattct aaagcggtca tttttttatt ttgttgaatt gcagtaatta ttaaattttg
     1921 cctttcagct ttattcatga cagccacctc ctaaaagatg atatattaca caaagtataa
     1981 cataagagtg acaatgagga cattttcaat atctttttaa aaaaatcgga aaaattttgt
     2041 ggaaaacgct tacattttaa aaaagattgt gctatgatat tacttgtaac agggggagcg
     2101 attaaacaaa ggggtagagc tatataacag atagctttta atcgttcaag ttacatcaca
     2161 acatcttcaa tatttattac ttactttcct ttctatttgt cggctagcac gtgactagcc
     2221 gattttttta tgcataaaat gaagaagtct tcagttgtat tgttgattct tttaatatag
     2281 ggtaataata aaatgataat gatatgagaa gtggtggacg aaatgacaga taaaacatta
     2341 cagcaaatag ataagttaat atgttcgtgg ttgaaacaaa tagacaatgt gattccacaa
     2401 ttaattatgg agatgactac agaaaccaag cgtcatcgat ttgatttagt tacaaatgta
     2461 gataaacaga ttcaacaaca atttcaacaa tttttagcaa cacattttcc ggaacaccaa
     2521 ctattagcgg aagaaaaaag taatgaaatg attacgaatg aaattaatca tttatggatt
     2581 atggatccca ttgatggaac tgccaattta gtaaaacagc aagaagatta ttgtattata
     2641 ttggcgtatt tttatgaagg taaaccaatg ttatcgtacg tatatgatta tccgcataaa
     2701 aagctttata aagcaatacg aggagaaggt gctttttgta atggatttaa gatggaagaa
     2761 ccaccatcgt taaaattgga agacgctatt atatcgttta atgcacaagt gatgaatctg
     2821 gatacggtgc aagatttatt tgacgcctca tttagttatc gtttagttgg tgcttgcgga
     2881 ttagattcta tgcgagtggc aaaaggtcaa tttggggcac atattaatac aaatcctaaa
     2941 ccttgggaca ttgctgcaca gtttttattt gcagaactat tgaatctaaa aatgacaact
     3001 ttagatggta aggcaattga tcatttaaag ggtgcacctt ttattataag taataaagca
     3061 tgtcatgaaa cggtacttaa aattttaaat gcaaatggtg gttatcaaaa atacagatag
     3121 atattctcga gagataaaaa atgataaaaa atagttatac gtctacagaa gtatagtaaa
     3181 aaaattattt tactatataa taaatagaga aaccagacaa agtatgtaag gaatagtaaa
     3241 ggagtggtgg gattgaaacg ttcgtctaaa agtaaaatga gcctaccgaa aaaaatattt
     3301 ttatgggtat tcggtatttt agtaatctta gcgattgtag ctgttgtgta tgtagctgct
     3361 aaaattttta ttactggtaa taagatacac aatccgttag atcgtaatca ttcagaatta
     3421 agagataaaa aagtgagttt aaacgatggt gatccattca caatcgcttt atttggtgtc
     3481 gattcagatg ctgatcgtaa gaaaaagggt ggcggagagc gtagtgatag tattatgatt
     3541 ttatctatca accctaaaac aaagaaaact gaaatagtaa gtataccgcg tgatacaaga
     3601 gcggaaattg ttggccgtgg tactactgaa aaaattgcac atgcctatgc atatggtgga
     3661 ccaaatatgg ctgttaaatc acttgaaaaa ttgatgaatg tgccaattga tcattatgcg
     3721 acaatcgata tggatggttt gcataatatg atagatagta tcggcggtgt tgatgtagta
     3781 agtaatgata cattcactgt tgatggtgtt cgttttacaa aaggacagca aacacatgta
     3841 aatggtgatc aagcattgaa gtttattaga agtcgtaaag aagaaggtgc tggtggtgac
     3901 tttggtcgac aacaacgtca gcaaattgta cttgaagcga tggctaataa gattgcaagc
     3961 ccatcatcga ttacacattt taatagtcta atgaatgaaa ttcaaaataa tgttaaaaca
     4021 gatttaacat taggtgattt gaatacgatt agaagcaatt ataaagatgc taatgacaca
     4081 attaataaac atcaattgag tggtcaaggc ggcattcaaa gtgacggatt gtactacttc
     4141 attcctagtg agcagtcaaa agctgaaagt actaagttat taaaagataa tctagagtag
     4201 taaactaagg gaaaataagt tatcaaattt gtacacaaat taattagata gaatgagctg
     4261 taaaaagaat aacgaataga attaatagcc cgtaaataga aggtgataac actttgtatt
     4321 tatgggctat tttatttaat gcctgatttg atttttacaa attattccat tacaaattta
     4381 atttaaagat aaatcttacc ttataaatgg cagtagatat ttatcaaact tgggttatta
     4441 attttcagaa aaagggtatt tataaatagt atagcgattt ttaggtgagg tgattattaa
     4501 gtattgtcat taataaggtt gtgaaagata tggcaaaaag cgtgacacag ataattggaa
     4561 aactaataat gataattatt ttcaattaaa caaaaagatg atttaatttc gcgcaaaata
     4621 tttcaaataa aaatgattgc aaaaataaaa aatacaaaaa tacgcttaac attatacact
     4681 taaagtaata caattatatt gattctatct gaaagatgtg tggggcatct ttattttagg
     4741 tggaaattag gcaatttatt aaaagtcatt tacggaaaaa tatatagatg gggtgaatag
     4801 tatgcaagaa catttggtgg tcacgcttga tggtaaagat tatcttgtag aaccaggtac
     4861 aaatttactt gaattcatta aatcgcaaga tacttttgtg ccttcaattt gttataacga
     4921 atcaatggga ccgatacaaa cgtgtgatac atgtacagtt gagattgatg gtaaaattga
     4981 acgttcatgt agtacagtga ttgatcgtcc aatgacagta aatacagtga ataatgacgt
     5041 gaaagatgct caaaaagaag cgcttgatcg aattttagaa aagcatatgc tgtattgtac
     5101 agtatgtgat tataataatg gtgattgtga aattcataat acgatggatg catgggggct
     5161 tcagcatcaa acgtatgaat ataaagagaa gccatatgaa aaagattatg gcccatttta
     5221 tcgctatgat ccaaatcaat gtatcttatg tggccgttgt gtagaagcgt gtcaagatat
     5281 tgaagtaaat gaaacaatta gaattgattg ggatcgtgag catccacgtg tcatttggga
     5341 taatgatgta ccgattaatg aatcgtcatg tgtatcttgt ggtcaatgcg caactgtatg
     5401 cccatgtaat gcgatgatgg aagttaacat ggaaggtaat gcaggttata tgactgatac
     5461 ggaacctggt tcattagcgg caatgattga tttaactaaa aaagcagaac ctggttatgg
     5521 tccactattt gcaatctcag attcagaagc agaaatgcgt aaagagcgta ttaagaaaac
     5581 taaaacagtg tgtacatatt gtggtgtggg ctgttcattt gaagtttgga ctaaagatag
     5641 agaaatttta aaagtacaac catcacatga ttctccagca aataaaattg caacttgtgt
     5701 taaaggtaag ttttcatggg gacatattaa ttcagatcaa cgcttaacga aaccattagt
     5761 aaggaaaaat ggtgagttcc atgaagtaga atgggatgaa gcattaaatg tcattgcaga
     5821 taattttaca tctattaaag aaaagcatgg tccagatgca ttatcattca tttcttcttc
     5881 taaagcgacg aatgaagaat cgtatttaat gcaaaaatta gcaagacaag ttattggcac
     5941 aaataacgtt gataactgtt caagatattg ccaagcacct gcaacaaaag gcttatttag
     6001 aacggttgga cacggcggtg actcaggtag tattgaagat ttagaaaaag cagcaatgtc
     6061 tgtattgatt ggtacgaata cagctgaagc acatccggtt attgcatcac gcatgaagcg
     6121 tgctcaaaaa ttatttggac aaaaaataca tgtgtttgat attagaaaac atgaaatggc
     6181 tgaacgcgca gatcgttttt atcaaccgaa accaggtaca gatttggctt ggttaagcgc
     6241 agtgacgaaa tatatcattg atcatgactt acacgataaa gcctttatcg aagagtgggt
     6301 tgaagatttt gatgaatatt acaagtcatt agaaacattt acaatggcat tcgctgaaga
     6361 ggctactggt attccagaag cagagttgat taagttcgct gaagaatgtg ccaaagctga
     6421 gtccgttgtc atttgttggg caatgggtat cacacaacaa gacattggaa gtgattcaag
     6481 tacagcaatt tcaaacttat tattagttac tggaaattat cgtcgacctg gtacaggcgc
     6541 gtatccatta cgtggacata ataatgttca aggttgtagt gatatgggta gtatgcctga
     6601 taaaattact ggctatcaaa gtattgaagc ggacgatatt cgcgctaaat tcgaaaaaga
     6661 atatggcgtt aaattgaatc cgaaagctgg taaagataat catgaaatgg ttgaaggtat
     6721 ccatgatggg caaattcact cattatatct atatggagaa gatactggta ttgtagattc
     6781 aaatattaat tttgtacaag ctgcttttga aaaattagat ttcatggtag tccaagatga
     6841 atttttaaca ttcacagcaa catatgcaga tgttgtcttg ccagcaagtc cttcattaga
     6901 aaaagatggt acttttacaa atacagagcg acgtattcaa cgtttatacc aagcattgaa
     6961 accattaggt gattctaaac ctgactggaa aatattccaa gcaattgcta ataaattaag
     7021 tttcgattgg aattacaaac atccaagcga aattatggat gaaattgcta gactaacacc
     7081 attatatgct ggcgtaagct acgaacgttt agaaggattt aatagtttac aatggcctgt
     7141 gcatccagat ggtacagatg aaccaatact ttatttagaa ggatttaatt tcgataatgg
     7201 taaagcaaaa ttattcccat tatcatttga taattacttt aagcaagatg aagtttatga
     7261 tattcatgta aacaatggtc gattgttaga gcacttccac gaaggtaaca tgacatatca
     7321 aacaccaatg cttaaataca aagtgccacg tgcatttgtt gaaatttctc cagaacttgc
     7381 tgaagataga ggcattcatg aaggtgcaga agttaagttg atatctgaaa caggagaagc
     7441 ggtattacaa gttcacgtga cagatcgtgt taaaggtaaa gaaatctata ttccattaaa
     7501 taatgatgcg atggaaaatg gagatctagg cgcaattaac ttattaacta atagtgatgt
     7561 tgatcaatac acggatacac catcttataa acgtacaagc tgtcgtatgg aggtaattac
     7621 gaaacgtggt aagtcaccgt tgaaccctaa taatttccgt gtcaataaaa aacgtcatcc
     7681 gcagtacagt gttcaagtac agaaaaaatg ggaacgtcca gattatgttt tcccgggaaa
     7741 tcaggtggat aaataatggc tgagagaata tcttctaaaa ttagacgctt agaaaaatca
     7801 gaagaacaaa ttaagttaga aagtttaaat gaagttacag aggcaattgc tgcaaacaaa
     7861 gatagcattt taaaagcaat caagctcatt aaaacattag atgatgcaaa actattagat
     7921 gctttaaatg gcgcagttag aggacgtcaa gtgattatta ataaatttgc tgttgagctc
     7981 aacaaagata tttatacagg gctattatct aatatggctt caatggtatt tttattaggc
     8041 gaattgaatg tatcagattt aagtgacttc cttaataaag ttaacaaagg tttacacgtt
     8101 gccaatcaag cgagtcctaa tgcgaagaca tctgtaagaa gtctattagg cgttttaaaa
     8161 gatgacgaca tgaaccgtag tttaacatat atgcttaata tgttaaaagg tatgtctcga
     8221 gaagattaat atgagtctat aataaatcgg attgatgtaa aagtttttta cttttcatct
     8281 ttccgatttt ttgtttttga atataacagt gtttctgcat gtcggtgtag gggcactgtt
     8341 ttaagttata ttgaaatatt atttgtatcc gagtcttcat caaacttcaa acaatatttt
     8401 tgagaatcga gaaaatcatg tgctattgat aatcaaccat cgacagggct atgaaacatc
     8461 atgtattatt atgattatgt aagttattta aataaaaagg gtgagtgcat attaattcga
     8521 gaatgttaaa aacgtcagct gtaatttcta atacattatt agtcataggt ttagtctgtc
     8581 tatttatgat gaaattagtg ttagctatta ctttttttgc tgtttcatta tcaattagtc
     8641 tagttgtctt taatatgatg ttcagagata gaacaacgat gaaagtaatc gttaacgctt
     8701 cttttttaat cgtaatatta gcgattgtag tggcatattt tttattgtct aagtaagaaa
     8761 aatgaggttt tgtctatatg aagaagaatt tcaagttacg tatttcaacg ctactattga
     8821 tagttatttt agttgttttt tctgtattgc tcatcgtgaa tgaaactaaa ttgtttaaaa
     8881 atgatgtgaa ttactctttt gatgaggctg tttcaatgca acaagggaaa ggtattgtac
     8941 agacaaaaga agaggatggt aaatttgttg aagcaaataa taatgaaatt gctaaagcaa
     9001 tgactatttc acataaagac aatgatatga agtatatgga tatcacagaa aaagtgccaa
     9061 tgtcggaatc tgaagttaat caattgctaa aaggtaaggg gattttagaa aatcgaggga
     9121 aagtttttct agaagctcaa gaaaaatatg aggttaatgt catttatctt gttagccatg
     9181 cattagtaga aacaggtaac ggcaaatcag aattagcaaa aggcattaaa gatggaaaaa
     9241 aacgctatta caactttttt ggtataggag cattcgatag tagtgctgtt cgtagtggga
     9301 aaagttatgc tgaaaaggaa caatggacat caccagataa ggcgatactt ggtggtgcaa
     9361 agttcattcg taatgaatat tttgaaaaca atcaactgaa tttatatcaa atgcgatgga
     9421 atccagaaaa tcctgcgcaa catcaatatg cgagtgacat tcgctgggca gataaaattg
     9481 ccaaattaat ggataaatcc tataagcagt ttggtataaa gaaagatgat attagacaaa
     9541 catattataa ataagacatc ggtgcttaaa ggggctggaa caatttattg tttcgagctc
     9601 ctttagcgca ttctgagtgt gttagttaaa tggattttaa cctaacataa aacgcgggag
     9661 tgggacagaa atgatatttt tacaaaattt atttcgttgt cccaccccaa cttgcattgt
     9721 ctgtagaaat tgggaatcca atttctcttt gttggggccc cgccaacttg cattgtctgt
     9781 agaaattggg aatccaattt ctctttgttg ggacccacac cccaactcgc attgcctgta
     9841 gaatttcttt tcgaaattct ctttgttggg gcccctgact agagttgaaa aaagcttgtt
     9901 gcaagcgcat tttcgttcag tcaactactg ccaatataat attatagacc ctaggacatt
     9961 gatttatgtc ccaagctccc acatcattgt tacaaatgta cattatgtaa atgaatattg
    10021 ctgtctaaat gtgcatgtaa tatacaatgg tgcagataat acacttaagt ctttaaaaat
    10081 gaaacgttag ttccaagagt catttttaaa caatagtgca tgtgataaaa tagaaaagaa
    10141 tgaaaaatat agaggtgaca atatgaagat agcaattata ggtgcaggca tcggtggatt
    10201 aacagctgct gcactattac aagaacaagg tcatactatt aaagtctttg aaaaaaatga
    10261 gtcagttaaa gaaattggcg ctgggattgg tatcggagat aatgtgctta aaaaactagg
    10321 taatcatgac ttagctaaag gtattaaaaa tgctgggcaa atcttatcta caatgacagt
    10381 gttagatgac aaagatcgcc cgttaactac agttaaatta aaaagtaata cattgaatgt
    10441 gacgttacca cgccaaactt taattgacat tattaaatct tatgtaaaag atgatgcaat
    10501 atttacaaat catgaagtca cgcatataga taatgagaca gataaagtta ccatacattt
    10561 cgcggaacaa gagagtgaag catttgattt atgtattggt gctgatggaa ttcattctaa
    10621 agtgagacaa tctgtaaatg ctgacagtaa agtattatat caagggtata catgctttag
    10681 aggtttaatt gatgatattg atttaaaaca tccggattgt gcaaaagaat actggggaag
    10741 aaaaggaaga gtaggtattg ttccgttatt aaataatcaa gcatactggt tcattacaat
    10801 taactcgaag gaaaacaatc ataaatatag ttcgtttggt aaacctcatt tgcaagcata
    10861 ctttaatcac tatccaaatg aagttagaga gatcttagac aaacaaagtg aaacaggtat
    10921 cttattgcat aatatttatg atttgaaacc actcaaatct tttgtttatg gtcgtactat
    10981 tttactagga gatgcagcac atgcgacaac gcctaatatg gggcaaggtg ctggacaagc
    11041 aatggaagat gctatcgtat tagtaaattg ttttaatgca tacgactttg aaaaagcatt
    11101 acagcgttat gataaaatac gtgtcaaaca tactgcaaaa gtaattaaac gttctagaaa
    11161 aatcggtaaa attgcccaat atcgtagtcg tttagttgtt gcagttagaa atcgtattat
    11221 gaaaatgatg ccaaatgcat tagcagctgg acagactaaa ttcttatata aatcgaaaga
    11281 aaagtaatac aacaatatga aaacccccgt atgttgaaat tagagctcaa catatggggg
    11341 gtcttgtttt tataatgtca ttataataaa ttcaattatt agttaacgac aaattgtggt
    11401 ttctcacctt gaacggcact aattgcagca ttagcaacaa ttttagacat catgtcacgt
    11461 gcttcaaatg tagcattacc aatatgcggt gttaatacta cattattaag tgattttaag
    11521 tcatcggtaa tgtctggttc gaattcatat acatcaagtg cagcaccttc aatttcatta
    11581 tttttcaatg cttgcactag tgcttgttcg tgcacgattg gaccacgaga ggcattgatt
    11641 aaatacgccg tagatttcat cattttaaat tgttctgtat caattaaatg atgcatttta
    11701 ggattgtaag cagcgttgat agtgataaaa tctgcattct ttaatagtgt atctaaatct
    11761 acatattttg caccgatttc tcgttctttt tcttctttgc gattaggtcc tgtgtatagt
    11821 acatccatgt caaatgctct tgcacgacgc gctactgcac taccaatttc acctaaaccg
    11881 ataatgccga ttgttttccc agatacttct ctacctctga aaaataaagg tgcccatccg
    11941 tcaaatccag ttgtacgtga taattggtcg ccttcaacaa tacgacgcgc tactgcaagt
    12001 actaatccaa ttgttaaatc agcagtcgcg tttgttgatg ctttaggtgt gtttgtaaca
    12061 tctatacttt tttctcgagc atactcgata tcaatattat taaaaccagc accatagttg
    12121 gcaatgattt ttaagtcttt accagcatcg ataacatctt tatcaacgtt tgtagataat
    12181 aaactaatta aggcagtcgc atctttaaca cctttaatta aagtgtcttt atcgactaat
    12241 cctttacctt catacatttc aacttcaaaa tgttcttgta aaagttttaa acctacttct
    12301 ggtattgcac cagcaacata aactttttcc ataaaagatc actcctttta tcttagtata
    12361 gtagaagatt agacagtata caactatgtc atgatgtctt gtgtatcaat gatgtaagcg
    12421 tgtacttttg atggaggtga tataacttag tcactgtaga actatgaata ttgtaatgtg
    12481 gaaaactgga tcaattaaat tagataacgt agttttaaag ttaatagtat tgaaaaaatt
    12541 aatattatga atatgggagg aaatatatat aagtaggtgg caatgaaaaa tagcaaaaaa
    12601 agagcttctc ctataaagga aagctcaaag tttttgatga catatgtact agaattaagt
    12661 ttcaagacaa tatgtatcat cgtgtttaaa ttaaatatgg atgtagttat agttacctgc
    12721 ttcacttgca gaaatagttc ttgagcttac tgagaaaggt ccgccactat aattcatttc
    12781 tgaaattgta actgaaccat cactgtttac actttctaca tatgcaacgt gaccaaatgg
    12841 tccttcagaa gattgtagga tagcgccttt agaaggtgta tggtttactg tgaatccagc
    12901 accttgtgca gcagcagccc aattattagc atttccccaa gtagatccga tttctccgcc
    12961 tactttatca tatacatacc aagtacattg tcctgcagtg tataagttac cagaatgtga
    13021 aattgatgat gtagttgtcg tagttgttgt agtcgttgta gtttgagtcg tattatagtt
    13081 ataatttgta taattttcag cagcgtctgc atgttgtgca tgacctacta atgctgtacc
    13141 aattcctgct gttaacgtag ttgctgttac taattttttc atgaataaag tcctccaaag
    13201 ttctatatct ttttttataa ataaaacgta gcgactgttt tattctcaca tctcgaattg
    13261 atgacaatag ttactttaac aaaattaatg cttcttgtgg ggaatgttat tgatttgtaa
    13321 aagaataaaa aaactttgac taattttgta ataaaaatta gtcaaagtta cagtgagatt
    13381 aacagataat taataggaaa tatttatttg taatatgttt aaataaatcg aattgttaaa
    13441 ggtattacat attcttggcc attataatat ttgacacacg caataattgt gaatacaaaa
    13501 gataaaattg agaaagcgaa tatggataaa ataccgataa acgtaatgat gaaacctata
    13561 atgataatga aatcaatatc ggtagcaatt aggaaaacgc ctataaaagt gataacgact
    13621 aaaacgatag accaaataat ataagaaatc gtatagttaa gataattttt tccagcacga
    13681 tcaactagtt tcgattcatc ttttttcaat aaccatataa tcagtggacc aataatagat
    13741 gtgaataaac ttaataaata gataagcatc gccataatgt tctcatcatt ggatttgcga
    13801 ttcggttgat gatttgttac gtcgttcatt tcagttgtca tattagacac tcctttgaaa
    13861 attgtaatat tatctttaac tataacaaaa tataatcaaa aataaacatg tttattaaac
    13921 aattattaaa aataaaaata atttgtggac gtcggcgttt aaataggtta atttaaggtt
    13981 atatatactt aacatttata atgatgcgta atgaattcgc atcattttta tattgtctta
    14041 cgtataattt gtttttaatt ttaaccaaag atagaaagag ggttgtttat gaaaatagca
    14101 attgtaggat caggaaatgg cgcagttacg gcagcagtag atatggtgag caaaggccac
    14161 gatgttaaat tatattgtcg taatcaatct ataagtaagt ttcaaaacgc aatcgaaaag
    14221 ggcggatttg attttaataa tgaaggtgat gaacgtttcg taaaattcac tgatattagt
    14281 gatgatatgg aatatgtttt aaaagatgct gaaattgttc aagtgattat tccatcttca
    14341 tacatagaat attatgctga tgtaatggca gagcatgtaa ctgataatca gttgatattc
    14401 ttcaacatgg ctgcagcaat ggggtcaatt cgttttatga atgttttaga agatagacat
    14461 attgaaacaa aaccacaatt agcagaagct aatacgttga cgtatggtac gcgtgtcgat
    14521 tttgaaaatg cagcagttga tttatctcta aatgtacgtc gtatcttctt ttcaacatat
    14581 gatagaagct gtctaaatga ttgttatgac aaagtttcaa gtatttatga tcatttagta
    14641 aaagaggaaa gcttaattaa aacaaatctt gaaaatggta atcctgaagt gcatccaggg
    14701 ccaacattat tgaatgtcgg tcgtattgat tatgctggtg agttcgcttt atataaagaa
    14761 ggaattacta aacatacagt tagattactt catgcaatcg aattagaacg tttaaattta
    14821 ggccgtagat taggttttga attatcaaca gctaaagaat cacgtattga acgtggttat
    14881 ttagaacgtg ataaagaaga tgaaccatta aatcgtttgt ttaatacaag cccagtattt
    14941 tcacaaattc caggaccaaa tcatgtagaa agcagatatt taactgagga tattgcatat
    15001 ggtttagtat tatggtcaag cttaggtcgt gttattgatg taccgacacc aaatatagat
    15061 gcagtaattg tcattgcatc aaccatttta gagagagact tctttgagga aggcttaaca
    15121 gttgaatcca ttggcttaga taagcttgat ttagaaaaat atttaaaata aatgatggct
    15181 tgaagataga aaaggatata gcattatgca aaagcaataa attgaagaaa agaggtttct
    15241 catcaataag cgaaggggac gatagatgat gacaagaaaa ccaacctttt tagaatcaat
    15301 ttcgacaatg attgtaatgg ttattgttgt tgtaacaggc tttgtgtttt ttgatattcc
    15361 aattcaagta ttattaatta ttgcctcagc atatgccgca tggattgcaa aacgtgtagg
    15421 cttaacatgg caagatttag aaacaggcat tgcagaacgt ttaaatactg caatgcctgc
    15481 aattttaatt atactagcgg taggaattat agtaggcagt tggatgtttt ctggcacagt
    15541 gccagccttg atttattatg gcttagattt attgaatcca agctattttt taatatcagc
    15601 cttttttata agtgctgtta catctgtagc aactggtaca gcatggggct ctgcatcaac
    15661 tgcagggatt gcacttattt ctatcggtaa tcaattaagt atctcaccag gaatggctgc
    15721 gggtgctatt atagcaggcg ctgtatttgg cgataaaatg tcacctttat cagatacaac
    15781 taatttagcg gcgcttgtca ctaaagtgaa tatatttaaa cacattcatt caatgatgtg
    15841 gacaacaata cctgcgtcaa tcatcggttt attagtatgg tttattgttg gatttcaatt
    15901 taaagggcat tcaaatgata agcagattca aacattgtta tcagagcttg cacagattta
    15961 tcaaattaac atatgggtct gggttccctt aattgtgatc attgtttgtt tgctatttaa
    16021 aatggctaca gtgccagcta tgctaatatc aagcttttct gccattatag tggggacttt
    16081 taatcatcat ttcaaaatga cagatggttt caaagcaaca tttagtggtt ttaacgactc
    16141 aatgatacat cagtctcata tttcattcag tgtgaaaagc ttgttagaac agggtggtat
    16201 gatgagtatg acccaaatat tagtaacgat attttgcgga tatgcatttg caggtattgt
    16261 agaaaaagca ggatgtttag aagtcttatt aactactatt tctaaaggca tccattctgt
    16321 aggaagttta atatgtatta ccgttatttg ttgtattgcg cttgtattcg ctgcaggtgt
    16381 tgcttcgatt gtaattatta tggtcggtgt gttaatgaaa gatttgttcg aaaaatacca
    16441 agtctctcgt tccgttttat caagaacact tgaagactca agtacgatgg ttttaccttt
    16501 aataccatgg ggaacatcag gtatttacta tacgaatcaa cttcatgtct ctgttggaga
    16561 atttttcata tggacagtac catgttattt atgcgcaatt atagcaatta tctatggttt
    16621 tacaggaata ggtattaaaa agtcatcgaa ttcacgttta acttaatgtg agcgtggaat
    16681 atatataata tgttgaaaca ctttaatcat ttataattgt agcggttata atttgaaaag
    16741 gttttaactt agaataaata tcctctatac atatactgaa tatgttttgt agcggaacat
    16801 gttaatatat gtaatgtaag ctttatgtca tggcatgtaa tgactaaatt aattgagaat
    16861 ttgaaggcaa gtatatttgt aagtacttta actaaaaatt tatcaatgta tagccgattt
    16921 gacgcgccta aatttgggtg tgtcaatggc tgtatgttgt ttattcttta ttacagagtg
    16981 aatcggattg gtggaaatcg aagttttgag atttttacca atccgatttt ttgatgtcta
    17041 gaaattaaaa aagccaacaa ggctcttgaa accttgttgg cttaattcac tttcatcaca
    17101 aattagtgaa tgaagttata accggcagct tggctagctg agattgtacg tgaagttaca
    17161 acacctgggc cataaccata gttcatttct gaaactctta ctgaaccatt gctgttaaca
    17221 ctttcaacgt atgcaacgtg accgtatgca ccttgagttg tttgcataat tgcaccagct
    17281 tttggtgtat tgttcactgt gtaaccagct cttgcagctg cgttagccca gttacttgca
    17341 ttgccccaag ttgaaccgat tttaccacct acacgatcaa atacgtagta tgtacattga
    17401 ccagaagtgt ataagttacg tcctgaagta taaccacttg agattgaacg gccatttgat
    17461 gatggagcca tagttgtagt tacttgaaca ttgttgcttg aagtgctgta gcttgcacct
    17521 aaaccaccag tacggtagct gtttgtgttg taactattat agttgttgta gttatatgat
    17581 tgattattat ttgagtagtt gttgtaacgg ctgtagttat tgtagctata accgttgttg
    17641 taattgttgt agctatagtt attgtagcca ttgtagtagt aatagctgta gtagccatta
    17701 tcttggttta attgacttgg atgccagtta cctttccatg tgtaatggta gttaccttgt
    17761 gcatcaatag tgtaagtata gctatatgat gttgggtcat ttggattata accgtagtta
    17821 tcttgctcag aagcatgagc ttgatttcct gatgcaattg cgattgtagc gaatcctgca
    17881 gttgcgatag tagctgtagc gattttcttc attttaaaaa tatcctccta aaaattttaa
    17941 atctaaaata ttttcgtaat gtccgtgtga caaaattaat gttataagtt atctctcgta
    18001 attaaacgac aagaaagact ataacagaaa ttagcgtcct tgtgtgcttt gttaacgttt
    18061 tgtaattttt tgctaatatc ttgacacaat agaattgtaa aagtatagaa atttgcattt
    18121 tgcaaaactt ataactacgg cattctttgt aaaaactgaa tgtttcgaaa ataagtatgt
    18181 tacaaatttg taatattact gaaaattcta aatgtatatt ttgtgcataa tataggactt
    18241 ttaatcagaa aatgcataaa aattcgacac gaacgatcaa atttctttgg ttaggaaagc
    18301 tttggtattt ggataaaaat gtatatttaa tggataaaat atcctaattt agcataaaaa
    18361 aatgttttaa taaaagtatt atttgatata atcgatttat gttttgttac tgctaaaaaa
    18421 catgtggcga tataattggt agtgaatatt agatgttgac aattgatttg agaaagacaa
    18481 acaggagtaa atcaatatat ttgtatatgt gaggatgtaa gatgacagat aattattcac
    18541 tacatatttt taaaagtatg aaaatgagta gtgtgaagga agcaaactta aaaattatat
    18601 attggcttca aggtacaggt gtagtttcaa tcaatctaca acaatatgat gtgaaaagaa
    18661 atgatgtcac cgttattatg ttgaatgatt tgtatcagat tgatggtcat gatgatagtg
    18721 tatgttgtgt tgttgaagta agtgcaaaga catttttgaa gtttatgaat gcaaactata
    18781 tgatgcgtgg caagatatta acaaatgagg ttgctagcac ttttagaatc ttgattaagt
    18841 atttaatcta cagcaaaatt aaacaacagc cttataatgc taatgataag attattaatt
    18901 atatgtgtgt tgaattaatg tcattaaata catacgatgg tagtcgtcat ttagtagccg
    18961 aagaagtgca cgattattta acaaacaacc accataaaaa gataaatagg aaagatgtca
    19021 ttaatcaagt tagtatcaca aacaaagctt taagtgacat gtttaaagcg acgccttata
    19081 gtaattttat tcaatattta aatcatatta gactggagca ttgcttaatt gatattttaa
    19141 cttctaagaa gcccattgaa gaaatcgcaa gtagtcatgg ttttaatcat tattctcgtt
    19201 ttatacacct ctttaaagaa acgtatggtg acactcctaa attaattaga aaaagaaata
    19261 gtccaacatc tcattcaatt aaccattccc aattagttga aattgataaa aatataattg
    19321 aattatttga tgatactaat caaagttctt caagtatgcg cgagattgat ataccttttg
    19381 agcctacaaa aaggagtcaa atgtatcaac ctaaaaacat ctatattaaa ggaagtaatt
    19441 tttattggat ggattactat actattaaac aaatcattca aagattaggt gttaatcctg
    19501 aaaacttgca tataattgtt acaatcgaca caagcaatga gctatcgatt gaacaaatca
    19561 ctttattact tcaaaagata gtgaattaca atgtaaatgt agtatttcgt attaaacatg
    19621 aatataagga attgttaacg agtactgaaa gaggcaagat agaaaagcta gtagctacaa
    19681 tttttaatat gacttttgag agtaatagat gtaagattgc atttttaatt aacgacttaa
    19741 atactactgc
//
LOCUS       DATTQF010000034        19675 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.034, whole genome shotgun sequence.
ACCESSION   DATTQF010000034 DATTQF010000000
VERSION     DATTQF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.034"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            176..1366
                     /locus_tag="VB241_001091"
     CDS             176..1366
                     /locus_tag="VB241_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEP1244394.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINKNTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFARDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1475..2719
                     /locus_tag="VB241_001092"
     CDS             1475..2719
                     /locus_tag="VB241_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEP1244395.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3058..3987)
                     /locus_tag="VB241_001093"
     CDS             complement(3058..3987)
                     /locus_tag="VB241_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP1244396.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNHIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DENVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNKNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4229..5608)
                     /gene="argH"
                     /locus_tag="VB241_001094"
     CDS             complement(4229..5608)
                     /gene="argH"
                     /locus_tag="VB241_001094"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEP1244397.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLSTYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5598..6803)
                     /locus_tag="VB241_001095"
     CDS             complement(5598..6803)
                     /locus_tag="VB241_001095"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEP1244398.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMRAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7154..8485
                     /locus_tag="VB241_001096"
     CDS             7154..8485
                     /locus_tag="VB241_001096"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP1244399.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8811..9386
                     /locus_tag="VB241_001097"
     CDS             8811..9386
                     /locus_tag="VB241_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP1244400.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVDTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLIVFWIVGKKLEQHFMGSKKE"
     gene            9391..9915
                     /gene="lepB"
                     /locus_tag="VB241_001098"
     CDS             9391..9915
                     /gene="lepB"
                     /locus_tag="VB241_001098"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP1244401.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKKLDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            9931..10506
                     /gene="lepB"
                     /locus_tag="VB241_001099"
     CDS             9931..10506
                     /gene="lepB"
                     /locus_tag="VB241_001099"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP1244402.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKYNFNPENTKN"
     gene            10666..14142
                     /gene="addB"
                     /locus_tag="VB241_001100"
     CDS             10666..14142
                     /gene="addB"
                     /locus_tag="VB241_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HEP1244403.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLESFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDALQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEISNELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQINPMLR
                     LLKTDVLTTSYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAATVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGNVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLSQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14143..17796
                     /gene="addA"
                     /locus_tag="VB241_001101"
     CDS             14143..17796
                     /gene="addA"
                     /locus_tag="VB241_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP1244404.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYETGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDISYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFHEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTLDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVRYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVFDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIIYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADT
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            17962..18864
                     /locus_tag="VB241_001102"
     CDS             17962..18864
                     /locus_tag="VB241_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEP1244405.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVERLYVFTKAASSLTGDNAKIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            19190..19579
                     /locus_tag="VB241_001103"
     CDS             19190..19579
                     /locus_tag="VB241_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HEP1244406.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMVLRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
BASE COUNT         6881 a         2861 c         3564 g         6369 t
ORIGIN      
        1 catttaagta attatgtcat tataaatgta agggttttca aaatagacaa atttaatgaa
       61 acttagtgat taattcatct tttcgggtga aaaagtatct gctttgaaaa ctaatgtaat
      121 tatttggata atgtttcatg tataatagtt ataaatgatg aaaaggggag atatgatgac
      181 taaatctgaa aaaattattg agttaacaaa tcattacgga gcacataatt atttaccatt
      241 gccaattgtc atttcagaag ctgaaggggt atgggttaaa gatcctgaag gcaataaata
      301 tatggatatg ttatctgcat attccgctgt taaccaaggt catagacatc cgaaaattat
      361 tcaagcatta aaagatcaag ctgataaagt gactctagtt tcacgtgctt ttcatagtga
      421 taacttagga gaatggtacg aaaaaatttg taaactggca ggtaaagata aagctttacc
      481 aatgaataca ggtgctgaag cagtagaaac agctttgaaa gcagcacgac gctgggcata
      541 cgatgttaaa ggaattgagc caaataaagc agaaatcatt gcatttaatg gtaacttcca
      601 tggtcgaaca atggcgccag tttcattatc atcagaagca gaataccaac gtggttatgg
      661 tccattatta gatgggttta gaaaagttga ttttggtgat gtagatgcat tgaaagctgc
      721 aattaataaa aatactgcag cagttttagt agaaccaatt caaggtgaag cgggtataaa
      781 tataccacca gaaggatatt tgaaagcaat tagagaatta tgtgatgaac ataatgtctt
      841 atttattgct gacgaaatcc aagcaggatt aggtcgttcg ggtaaattat ttgctaggga
      901 ttgggataat gtaaaacctg atgtctatat tttaggtaaa gcactaggtg gtggtgtctt
      961 cccaatttct gttgtattag cagataaaga agtattagat gtctttacac ctggctcaca
     1021 tggttcaaca tttggtggta atccacttgc ttgtgcagca tcaattgctg cattagatgt
     1081 tatcgttgat gaggatttac caggtcgctc tttagaatta ggagattatt ttaaagaaca
     1141 attaaagcaa attgatcatc catcaattaa agaagtccgt ggacgtggtt tgtttatagg
     1201 tgtggaactt aatgaaagtg ctagaccata ctgtgaagca ttaaaagaag aaggcttatt
     1261 atgtaaagaa acgcatgata ctgtcattcg ttttgcacca ccattaatta ttactaaaga
     1321 agaattggac cttgcacttg aaaaaataag acatgtattt caataatgtc aaaaagttgt
     1381 aacaatcagt ttgtaagggc tttaaaaagg aatacgttat gttacaatat gtttgaaagc
     1441 gaaatcatta ggtaaagaag aggcgaaaag gatcatgact gagaacaata atttagtaac
     1501 ttctactcaa ggaattatta aagaagcatt gcataaattg ggatttgacg aaggaatgta
     1561 cgatttaatt aaagaacctt taagaatgtt acaagtgcgt atccctgtac gaatggatga
     1621 tggcactgtt aaaacattca caggttaccg tgcgcaacat aatgatgctg ttggaccaac
     1681 aaaagggggc gtgcgtttcc acccagatgt tgatgaagaa gaagtaaaag cattatcaat
     1741 gtggatgact ttgaaatgtg gcattgttaa cttaccatac ggtggtggta agggtggtat
     1801 cgtttgtgat ccacgtcaaa tgagcattca tgaagttgaa cgtttatcac gcggatatgt
     1861 aagagcaatt tcacaattcg taggtccgaa caaagatatt ccagcaccag atgtatttac
     1921 aaactcacaa attatggctt ggatgatgga tgaatatagt gcattagata aatttaattc
     1981 accaggtttc atcacaggta aaccaattgt attgggtggt tctcatggac gcgatagatc
     2041 aactgcacta ggtgtagtta ttgcaattga acaagctgca aaacgtcgta atatgcaaat
     2101 tgaaggtgcc aaggttgtta ttcaaggttt cggtaatgcc ggaagtttct tagctaaatt
     2161 cttatatgat ttaggtgcaa aaattgtagg tatctctgat gcttacggtg cattacacga
     2221 tccaaatggc ttagatatag attatttatt agaccgtcgt gatagttttg gtacggtaac
     2281 aaatttattt gaagaaacaa tctcaaataa agaattgttt gaattagatt gtgacatttt
     2341 agtaccagcg gctatttcaa accaaattac agaagacaat gcacatgata ttaaagctag
     2401 catcgttgtt gaagctgcta atggacctac aacaccagag gcaacacgta ttttaactga
     2461 acgtggtata ttattagttc cagacgtatt agcaagtgct ggtggtgtaa cggtttcata
     2521 cttcgaatgg gtacaaaata atcaaggtta ttattggtct gaagaagaag ttaatgaaaa
     2581 gctacgtgaa aaattagaag cggcatttga tacgatttac gaattgtctc aaaatcgaaa
     2641 aatagatatg agacttgcag catatatcat aggtattaaa cgtacagcag aagcagctag
     2701 atatcgtggt tgggcataat taattatcat atgtgattta acgagcttgg gacagaaaac
     2761 aaagccctaa gctcgttaat tatattttag cagtagttga ctgaataaaa atacacttgt
     2821 aacaagctta tttcaaaaat agccggggcc ccagcacaga agctggcgaa aagtcagctt
     2881 acaataatgt gcaagttggg gtgggccccc aacacagaga atttctaatt aaacgctcaa
     2941 ctatatatag ttgagcaaaa agacagcagt aggataattt caatttggaa atatcttact
     3001 gctgtttttt atagtaatag ctatattttt gtatttatgt tttctagttt aacaatacta
     3061 tttaatgact tctttatatt tatcagcgaa atttgtaaag acaccatcaa cgccgtattt
     3121 atttaatcgt aacatatcag ctttttcatt cactgtataa ggatgtacta taaatcctaa
     3181 gtcttttaaa tgatgggtat tttgttcagt taaatctgta taatcaggac ctaatccaat
     3241 cgcataagag cgtatctctt ttaagcgttg gtcgttaaat tgttgtagtt cacctttatc
     3301 aactaatttt actaatggca catgcttatt ttgacgatga attttcttta aactttcgtc
     3361 agaaaatgat tgaatcatta catgtccatt ttttaattta tttttattta aaaggtgatg
     3421 ctttttcaat gaagctaata attgttcttc cattcctggg tatacatcag gtgactttgt
     3481 ttcaatataa tagtttgcat ttggaccata acgttctaaa atttcatcta aagtgggtac
     3541 tttagcattt ttataacttg ctcttgcgta ttttggatat tttttattaa accaacttcc
     3601 tgcatctaac tgttttaatt catcaagggt ataatcctca actttaccgt gtccatttgt
     3661 tgtacggtta acattttcat catgcatagc aactaaatgg ccatctttgg tacgttgtaa
     3721 atcaatttcg atatacgatg cttttaactc attatgactc ttatcatatg cttgaaacgt
     3781 atgctcgggt gcatagccac ttgcgccacg atgtgcgata gtagtgaatc gctcattcgt
     3841 taaatttgta tgccattgaa tagcctgagg tttatttgca atatgattag tttgttcagc
     3901 gccagcagta ggtactgata aaaatcccat agtaaaaaca gcagaagcag ccataaattt
     3961 agtgaagctt ttcgaagagt tagtcatagt ctttatcctt tcatctcttt atttgtagtc
     4021 taacaatttt aaaatactac aaaaacctgc ttgtgaaaac gctttacaca tttcttttac
     4081 aatttataac atttctgtaa tataaattta aaagaattat caaacatttt aggaatctta
     4141 aaaatgtgat gttcaacaag acgaagagag cattaaaata cccacaatgt aattttcata
     4201 gacattgtgg gtaggtagat taacgtattt attgtgatag taattgttta gcaacatcaa
     4261 gttgttgttt gaccgatgat tgacccgtag aaccgtaact ttgacgtcgt tttaagcaat
     4321 tttcaggctg caaataatcg taaatatcgg catcgatact agaatgatgt tgttgatatg
     4381 ttgataaagg aacatctaat aaataatgac cttgttgtat acattctaag acgatttttc
     4441 ctacaatttc atgtgcagtt ctaaatggaa tatttttagt tactaaataa tctgctagtt
     4501 ccgttgcatt tgaaaaatct tctttaacag tttgattgag tcgttcttta ttaatggtca
     4561 tcgtttgaat cataccttcg aaaatacgta aagaaccttt aattgtatgg acagcatcga
     4621 ataaaccttc tttatcttcc tgcatatctt tgttatatgc tagaggtaat ccttttaaag
     4681 tcataagcat gctcattaaa tgaccagtcg ttcgaccaac tttacctcta attaattctg
     4741 ccatatcagg atttttcttt tgtggcataa tagatgagcc agtagaaaat gcatctgata
     4801 atgtaatgaa tttagcttcg tctgtggacc agaaaataat ttcctctgca aaacgtgata
     4861 agtgaaccat cgttaaagaa atattatgca atgtttcaat aatatagtct ctgtcactaa
     4921 cagcatctag gctattctca tagaggctgc caaagttcaa caatgctgtt gtctcgtgtc
     4981 tatcgatagg gtatgtggta ccacttaagg ctgctgcacc taaaggatta atatcgattc
     5041 gttttaaact atcttcaaat cgttgttggt ctcgttgtaa catccaaaaa taagtcataa
     5101 tatgatgtgc aaatgaaatt ggctgtgcac gctgtaaatg agtataacca ggcataattg
     5161 tatcaacatt attggaagcg atgtctacaa ttacactttg taacgactta attaatgcga
     5221 tgatatcttg cacttgtttc ttagtgtaca agtgcatgtc tgttgcaact tgatcgtttc
     5281 tactgcgtcc agtatgcaac ttaccaccag catcaccaat acgtttaatt aattcatgtt
     5341 caatatttaa atgaatatct tctaatgatg cactaaattg aatttgatct tgatgataat
     5401 catgttgaat agattttagt ccttgtataa tttgttcgct gtcttgttga ctaataatgc
     5461 cttgattcgc aagcatagtt gcatgcgcaa tgctgccttc gatatcttga tctatgagcg
     5521 tttgatcaaa agtaatggat gcgttaaagt cgtcaaccca ctcttcaggt tgtacttcaa
     5581 atctaccgcc ccaagcttta ttgctcattg ctatagcctc catgtaacat cgcatttact
     5641 tgagtaggta aaccatagat atcgataaag ccaacagcag cgtcttgatt aaatgcatct
     5701 tcttttgtat aagttgctaa tttttcatca tataatgtgt aaggtgattt tctaccattc
     5761 acgatagcat tacctttgaa taatttaatt ctgacatcac cacttacgta ttgctgagta
     5821 ctatcaataa ataatttcaa gctatcagtt aaaggtgaga accaaagtcc attgtatagt
     5881 tgttcagcaa attgcttctc aatgattggt ttaaagtgtg caacatcttt cgttaacgta
     5941 atcgtttcta atgctttatg tgcttttaaa ataacttctg cagcaggtgc ttcataaatt
     6001 tctcttgatt tgatacctac aagtctattt tctacatggt caattcttcc gataccatgc
     6061 ttaccagcta atgcattcaa cgttaaaatt aaatcgtcta attcatatgt tttgccatca
     6121 atttgaactg ggatgccttt atcaaacgtt aaaatgattt catcagcagt atctggtgtt
     6181 tcttctaaag catttgttag atcgaacgca tcctctggtg gtgcagcata aggatcttct
     6241 aaaataccac attcattcgc tctgccccat aggttttgat cgatagaata aggtgaatca
     6301 tggttaattg atacagggat attatgttta attgcataat caatttcttc ttcacgactc
     6361 catgcccact cacgtacagg tgcgaatgct ttcaatgatg ggtttaatgc tttaatggca
     6421 acttcgaaac gtacttggtc attcccttta ccagtacaac catgtgcaat acctactgaa
     6481 tttgttttct cagcaatctc tactaatttt ttagcgatta atggtcttga taaagcagaa
     6541 actaatggat atgcattttc atacattaaa tttcctttga ttgcataact tacatactca
     6601 tcactaaatt cttttgttgc atcaataata tgacattcaa cagctcgcat atctaaagct
     6661 tttttataaa cgatgtctaa atctttacct tcaccaacat ctaggcaaca agctacaacg
     6721 tcgtatcctt tgtcgataag ccattgaacg gccacacttg tatctagtcc tcctgaatat
     6781 gctaaaacaa ttttctcttt cataaaattc acctcattgt agttatctga atatttcgta
     6841 tgaacacatg atagcaaact tttaatgaaa ataaaaccat tattttacat atttattcat
     6901 aaaatatgat agaggtagtt agagttgtaa gtataaatac ttatttatca atgtttaaaa
     6961 gtgttttaaa aaacatgcaa attgtataaa aaatgtttga gtgtttgacg ccggtttgaa
     7021 atgtttgttg aaaaataggt gtgaaacgtc acataaatat caattttatt gattaagtac
     7081 aaacttgtga attgaatcgc aaacgtaaac ttagtaaaat taagatatta aatgaaacag
     7141 gaggctttta tagatgactc atattcaatt agattttagt aaaacgttag aatttttcgg
     7201 tgaacacgaa ttaaaacaac aacaagaaat tgttaaatca attcacaaaa caattcatga
     7261 aggtactggt gcaggtagtg acttcttagg ctgggttgat ttaccagttg attacgacaa
     7321 agaagaattt tcaagaattg ttgaagcatc aaaacgcatt aaagaaaatt ctgatgtttt
     7381 agtagtcatc ggtattggtg gttcttactt aggtgcacgt gcagcaatcg aaatgttaac
     7441 gtcatcattt agaaacagca atgaataccc tgaaattgta tttgttggta atcacttatc
     7501 atcaacatac actaaagagt tagttgatta tttagcagac aaagatttct ctgtaaacgt
     7561 tatttctaaa tctggtacaa ctacagaacc agcagttgca tttagattgt tcaaacaatt
     7621 agttgaagaa agatacggta aagaagaagc acaaaaacgt atatttgcaa caacggataa
     7681 agaaaaaggt gctttaaaac agttggctac aaacgaaggt tatgaaacgt ttatcgtacc
     7741 tgatgatgta ggtggaagat attctgtttt aacagcagta ggattattac caattgcaac
     7801 agctggaatt aacatcgaag ctatgatgat tggtgctgca aaagcacgtg aagaattatc
     7861 ttcagataaa ttagaagaaa acattgcata ccaatatgcg acaattcgaa acattttata
     7921 tgcaaaaggt tatacaacag aaatgttgat taactatgaa ccatctatgc aatactttaa
     7981 tgaatggtgg aaacaattat ttggtgaatc agaaggtaaa gacttcaaag gtatttatcc
     8041 ttcaagtgcc aactacacaa ctgatttaca ttctttaggt caatatgtac aagaaggccg
     8101 tcgtttctta ttcgaaacag ttgtaaaagt aaatcatcct aaatatgata ttactattga
     8161 aaaagatagt gatgatctag acggattaaa ttatttagct ggtaaaacaa tcgatgaagt
     8221 taacacaaaa gcattcgaag gtacattatt agcgcatact gatggtggtg ttcctaacat
     8281 ggtagtgaac attccacaat tagatgaaga aactttcggt tatgtcgtat acttcttcga
     8341 acttgcttgt gcaatgagtg gataccaatt aggcgtaaat ccatttaacc aacctggtgt
     8401 agaagcatat aaacaaaaca tgttcgcatt attaggtaaa cctggttttg aagacttgaa
     8461 aaaagaatta gaagaacgtt tataaaatac attacttcaa tgattagtga agtttgaaaa
     8521 gatagaacta gacgttaact atttaaagca tatttccgag gttgtcatta caaatgtaaa
     8581 aatgtaatga caacctcgtt tttattttat atgcaagata taggttacta gctaatgtga
     8641 caagatgtta agagaaaatt aaagagaaaa taatatctgc catacaataa aattgttata
     8701 ctactagaga ctgatttatt agcatgatta catgttaatg tttctttact tagtaattaa
     8761 ctttataatg taagaataat tatcttcagc caaagaaagg gattgatgat ttgtcgtttc
     8821 atcaagtaga agaatggttt gagatatttc gacagtttgg ttatttacct ggattcatat
     8881 tgttatatat aagagcgatt attccagtat ttccattagc actctatatt ttaattaata
     8941 ttcaagccta tggacctatt ttaggtatat tgattagttg gcttggatta atttctggaa
     9001 catttacagt ctatttgatc tgtaaacgat tggtggatac tgaaaggatg cagcgaatta
     9061 aacaacgtac tgctgttcaa cgcttgatta gttttattga tcgccaagga ttaatcccat
     9121 tatttatttt actttgtttt ccttttacgc caaatacatt aataaatttt gtagcgagtc
     9181 tatctcatat tagacctaaa tattatttca ttgttttggc atcatcaaag ttagtttcaa
     9241 caattatttt aggttattta ggaaaggaaa ttactacaat tttaacgcat cctttaagag
     9301 ggatattaat gttagttgtg ttgattgtat tttggattgt tggaaaaaag ttagaacagc
     9361 attttatggg gtcgaaaaag gagtgacatc gtgaaaaaag ttgtaaaata tttgatttca
     9421 ttgatacttg ctattatcat tgtactgttc gtacaaactt ttgtaatagt tggtcatgtc
     9481 attccgaata atgatatgtc accaaccctt aacaaagggg atcgtgttat tgtaaataaa
     9541 attaaagtta catttaatca attgaataat ggtgatatca ttacatatag gcgtggtaac
     9601 gagatatata ctagtcgaat tattgccaag cctggtcaat caatggcgtt tcgtcaggga
     9661 caattatacc gtgatgaccg accggttgac gcatcttatg ccaagaacag aaaaattaaa
     9721 gattttagtt tgcgcaattt taaaaaatta gatggagata ttataccgcc taacaatttt
     9781 gttgtgctaa atgatcatga taacaatcag catgattcta gacaatttgg tttaattgat
     9841 aaaaaggata ttattggtaa tataagtttg agatattatc ctttttcaaa atggacgatt
     9901 cagttcaaat cttaaaaaga ggtgtcaaaa ttgaaaaaag aattattgga atggattatt
     9961 tcaattgcag tcgcttttgt cattttattt atagtaggta aatttattgt tacaccatat
    10021 acaattaaag gtgaatcaat ggatccaact ttgaaagatg gcgagcgagt agctgtaaac
    10081 attattggat ataaaacagg tggtttggaa aaaggtaatg tagttgtctt ccatgcaaac
    10141 aaaaatgatg actatgttaa acgtgtcatc ggtgttcctg gtgataaagt agaatataaa
    10201 aatgatacat tatatgtcaa tggcaaaaaa caagatgaac catatttaaa ctacaattta
    10261 aaacataaac aaggtgatta cattactggg actttccaag ttaaagattt accgaatgcg
    10321 aaccctaaat caaatgtaat tccaaaaggt aaatatttag tgcttgggga taatcgtgaa
    10381 gtaagtaaag atagccgtgc gtttggcctc attgatgaag accaaattgt tggtaaagtt
    10441 tcgtttagat tctggccatt cagcgaattt aaatataatt tcaatcctga aaatactaaa
    10501 aattaatatg aaacaaatac aacatcgttt gtcggtttta atactgataa acgatgtttt
    10561 attttgtaat aggtatgata aaagctaagt tcgaaatgaa cttataataa atcaatcaca
    10621 atcactttgt gttaaaatat gtgtcaaagg aagtgagggt ttgtcatgac attacatgct
    10681 tatttaggta gagcgggaac aggtaagtct acgaaaatgt tgaccgaaat aaaacaaaaa
    10741 atgaaagcag atccgcttgg agatccaatc attttaattg cgccaactca aagtacattt
    10801 caattagaac aagcctttgt caatgatccg gaattaaatg gtagtttaag aacagaagtg
    10861 ttgcattttg aacgattaag tcatcgtatt ttccaagaag ttggtagtta tagcgaacaa
    10921 aagttatcta aagctgcaac ggaaatgatg atttataaca ttgttcaaga acaacaaaag
    10981 tatttaaaac tttatcaatc acaagcaaaa tattatggat ttagtgaaaa attaacagaa
    11041 caaattcaag attttaaaaa atatgcagta acgcctgaac atttagaaag ctttattgct
    11101 gataaaaata tgcaaactcg aactaaaaat aagttagagg atattgcttt aatataccga
    11161 gagttcgaac aacgcattca aaacgagttt attacgggtg aggatgcatt acaatatttt
    11221 attgattgta tgccgaaatc agagtggcta aaacgtgctg atatatatat tgatggtttt
    11281 cacaactttt caacgattga gtatttaata atcaaaggat taattaaata tgcgaagagt
    11341 gtcacaatta tattgacgac agatggtaac cacgatcaat tcagtttatt tagaaaacca
    11401 tcggaagtgt tacgacatat tgaagaaata tcaaatgaac tcaatatttc tattgaacgt
    11461 caatatttca accaattata tcgctttaat aatcaagatt taaagcatct tgaacaagaa
    11521 tttgatgcac ttcaaatcaa tcgagtggca tgtcaaggtc atatcaatat tttagaatct
    11581 gcgactatga gagaggaaat aaatgaaatt gcacgacgta tcatcgttga tattcgtgat
    11641 aaacaattac gatatcaaga tattgctatt ttatatcgtg acgagtctta tgcttattta
    11701 tttgattcca tattaccgct ttataatatt ccttataaca ttgatacaaa gcgttcgatg
    11761 acacatcatc cggtcatgga aatgattcgt tcattgattg aagttattca atcaaattgg
    11821 caaattaatc caatgctacg cttattgaag actgatgtgt taacgacatc atatctaaaa
    11881 agtgcatact tagttgattt acttgaaaat tttgtacttg aacgtggtat atacggtaaa
    11941 cgttggttag atgatgagct atttaatgtc gaacatttta gcaaaatggg gcgtaaagcg
    12001 cataaactta ccgaagatga acgtaacaca tttgaacaag tcgttaagtt aaagaaagat
    12061 gtcattgata aaattttaca ttttgaaaag caaatgtcac aagcagcaac tgtaaaagac
    12121 tttgcaactg ctttttatga aagtatggaa tattttgaac tgccaaatca attgatgaca
    12181 gagcgagatg aacttgattt aaatggtaat catgaaaagg cggaagaaat tgatcaaata
    12241 tggaatggct taattcaaat cctcgatgac ttagttctag tatttggaga tgaaccaatg
    12301 tcgatggaac gtttcttaga agtatttgat attggtttag aacaattaga atttgttatg
    12361 attccgcaaa cattggacca agttagtatt ggtacgatgg atttggctaa agtcgacaat
    12421 aagcaacatg tttacttagt tggaatgaat gatggtacga tgccacaacc agtaactgcg
    12481 tcaagcttga ttacagatga agaaaagaaa tactttgaac agcaggctaa tgtcgagtta
    12541 agtccaacat cagatatttt acagatggat gaagcatttg tttgttatgt tgctatgact
    12601 agagctaagg gaaatgttac attttcttat agtctaatgg gatcaagtgg tgatgataag
    12661 gaaatcagcc catttttaaa tcaaattcaa tcattgttca accaattgga aattactaac
    12721 attcctcaat accatgaagt taacccattg tcactaatgc aacatgctaa gcaaaccaaa
    12781 attacattat ttgaagcatt gcgtgcttgg ttagatgatg aaattgtggc tgatagttgg
    12841 ttagatgctt atcaagtaat tagagatagc gatcatttaa gtcaaggttt agattattta
    12901 atgtcagcat taacgtttga caatgaaact gtaaaattag gtgaaacgtt gtctaaagat
    12961 ttatatggta aggaaatcaa tgccagtgta tctcgatttg aaggttatca acaatgccca
    13021 tttaaacact atgcctcaca tggtctgaaa ctaaatgagc gaacgaagta tgaacttcaa
    13081 aactttgatt taggcgatat tttccattcc gttttaaaat atatatctga acgtattaat
    13141 ggtgatttta aacaattaga cctgaaaaaa ataagacaat taacaaatga agcattggaa
    13201 gaaattttac ctaaagttca atttaattta ttaaattctt cagcttacta tcgttattta
    13261 tcaagacgca ttggcgctat tgtagaaaca acactaagcg cattaaaata tcaaggcacg
    13321 tattcaaagt ttatgccaaa acattttgag acaagtttta gaaggaaacc aagaacaaat
    13381 gacgaattaa ttgcacaaac attaacgaca actcaaggta ttccaattaa tattagaggg
    13441 caaattgacc gtatcgatac gtatacaaag aatgatacaa gttttgttaa tatcattgac
    13501 tataaatcct ctgaaggtag tgcgacactt gatttaacga aagtatatta tggtatgcaa
    13561 atgcagatga tgacatacat ggatatcgtt ttacaaaata aacaacgcct tggattaaca
    13621 gatattgtga aaccaggtgg tttattatac ttccatgtac atgaacctag aattaaattt
    13681 aaatcttggg cagatattga tgaagataaa ttagaacaag atttaattaa aaagtttaag
    13741 ctgagtggtt tagtgaatgc agaccaaact gttattgatg cattggatat tcgtttagaa
    13801 cctaaattca cttcagatat tgtaccagtt ggtttgaata aagatggttc tttgagtaaa
    13861 cgaggcagcc aagttgcaga tgaagcaacg atttataaat tcatccaaca taacaaagaa
    13921 aattttatag aaaccgcttc aaatattatg gatggacata ctgaagttgc accattaaag
    13981 tacaaacaaa aattgccatg tgctttttgt agttatcaat cggtatgtca tgtagatggc
    14041 atgattgata gtaagcgata tcgaactgta gatgaaacaa taaatccaat tgaagcaatt
    14101 caaaatatta acattaatga tgaatttggg ggtgagcaat agatgacaat tccagagaaa
    14161 ccacaaggcg tgatttggac tgacgcgcaa tggcaaagta tttacgaaac tggacaagat
    14221 gtacttgttg cagccgcggc aggttcaggt aaaacagctg tactagttga gcgtattatc
    14281 caaaagattt tacgtgatgg cattgatgtc gatcgacttt tagtcgtaac gttcacaaac
    14341 ttaagtgcac gtgaaatgaa gcatcgtgta gaccaacgta ttcaagaggc atcgattgct
    14401 gatcctgcaa atgcacactt gaaaaaccaa cgcatcaaaa ttcatcaagc acaaatatct
    14461 acacttcata gtttttgctt gaaattaatt caacagcatt atgatgtatt aaatattgac
    14521 ccgaacttta gaacaagcag tgaagctgaa aatattttat tattagaaca aacgatagat
    14581 gaggtcatag aacaacatta cgatatcctt gatcctgctt ttattgaatt aacagagcaa
    14641 ttgtcttcag atagaagtga tgatcagttt cgaatgatta ttaaacaatt gtatttcttt
    14701 agcgttgcaa atccaaatcc tacaaattgg ttggatcaat tggtgacacc atacgaagaa
    14761 gaagcacaac aagcgcaact tattcaacta ctaacagact tatctaaagt atttatcaca
    14821 gctgcctatg atgctttaaa taaggcgtat gatttgttta gtatgatgga tggcgtcgat
    14881 aaacatttag ctgttataga agatgaacga cgtttaatgg ggcgtgtttt agaaggtggc
    14941 tttattgata tatcttattt aactggtcac gaatttggtg cgcgtttgcc taatgtaaca
    15001 gcgaaaatta aagaagcaaa tgaaatgatg gtcgatgcct tagaagatgc taaacttcag
    15061 tataaaaaat ataaatcatt aattgataaa gtgaaaagtg attacttttc aagagaagct
    15121 gatgatttga aagctgatat gcaacaattg gcgccacgag taaagtacct tgcgcgtatt
    15181 gtgaaagatg ttatgtcaga attcaatcga aaaaagcgta gcaaaaatat tctggatttt
    15241 tctgattatg aacattttgc attacaaatt ttaactaatg aggatggttc gccttcagaa
    15301 attgccgaat cataccgtca acattttcat gaaatattgg tcgatgagta tcaagatacg
    15361 aaccgagttc aagagaaaat actatcttgc atcaaaacgg gtgatgaaca taatggtaat
    15421 ttatttatgg ttggagatgt taagcagtcc atttataaat ttagacaagc tgatccaagt
    15481 ttatttattg aaaagtatca acggtttact ttagatggag atggcactgg gcgtcgaatt
    15541 gatttgtcgc aaaacttccg ttctcgtaaa gaagtactgt caacgacaaa ctatatattc
    15601 aaacatatga tggatgaaca agtcggtgaa gtaagatatg atgaagcagc acagttgtat
    15661 tatggtgcac catatgatga atcggaccat ccagtaaact taaaagtgct tgttgaagcg
    15721 gatcaagaac atagtgattt aactggtagt gaacaagaag cgcattttat agtagaacaa
    15781 gttaaagata tcttagaaca tcaaaaagtt tttgatatga aaacaggaag ctatagaagt
    15841 gcgacataca aggatatcgt tattctagaa cgcagctttg gacaagctcg caatttacaa
    15901 caagccttta aaaatgaaga tattccattc catgttaata gtcgtgaagg atactttgaa
    15961 caaacagaag tccgtttagt attatcattt ttaagagcga tagataatcc attacaagat
    16021 atttatttag ttgggttaat gcgttccgtt atttatcagt tcaaagaaga tgaattagct
    16081 caaattagaa tattgagtcc gaatgatgac tacttctatc aatcgattgt aaattacatt
    16141 aatgacgaag cagcagatgc tattttagtt gataaattaa aaatgttttt atcagatatt
    16201 caaagttacc aacaatatag taaagatcat ccggtgtatc agttaattga taaattttat
    16261 aatgatcatt atgttattca atactttagt ggacttattg gtggacgtgg acgacgtgca
    16321 aatctttatg gtttatttaa taaagctatc gagtttgaga attcaagttt tagaggttta
    16381 tatcaattta ttcgttttat cgatgaattg attgaaagag gcaaagattt tggtgaggaa
    16441 aatgtagttg gtccaaacga taatgtcgtt agaatgatga caattcatag tagtaaaggt
    16501 ctagagtttc catttgtcat ttattctgga ttgtcaaaag attttaataa acgtgatttg
    16561 aaacaaccag ttattttaaa tcagcaattt ggtctcggaa tggattattt tgatgtggat
    16621 aaagaaatgg catttccatc tttagcttcg gttgcatata aagctgttgc cgaaaaagaa
    16681 cttgtgtcag aagaaatgcg attagtctat gtagcattaa caagagcgaa agaacaactt
    16741 tatttaattg gtagagtgaa aaatgataaa tcattactag aactagagca attgtctatt
    16801 tctggtgagc acattgctgt caatgaacga ttaacttcac caaatccgtt ccatcttatt
    16861 tatagtattt tatctaaaca tcaatctgcg tcaattccag atgatttaaa atttgaaaaa
    16921 gatatagcac aaattgaaga tagtagtcgt ccgaatgtaa atatttcaat tatatacttt
    16981 gaagatgtgt ctacagaaac cattttagat aatgatgaat atcgttcggt taatcaatta
    17041 gaaactatgc aaaatggtaa tgaagatgtt aaagcacaaa ttaaacacca acttgattat
    17101 caatatccat atgtaaatga tactaaaaag ccctcaaaac aatctgtttc tgaattgaaa
    17161 agacaatatg aaactgaaga aagtggcaca agttacgaac gagtaaggca atatcgtatc
    17221 ggtttttcaa cgtatgaacg acctaaattt ctaagtgaac aaggtaaacg aaaagcgaat
    17281 gaaattggta cgttaatgca tacagtgatg caacatttac cattcaaaaa agaacgcata
    17341 tctgaagttg agttacatca gtatatcgat ggattaatcg ataaacatat tatcgaagca
    17401 gatacgaaaa aagatatccg tatggatgaa ataatgacat ttatcaatag tgagttatat
    17461 tcgattattg ctgaagcaga gcaagtttat cgtgaattac cgtttgtagt taaccaagca
    17521 ttagttgacc aattgccaca aggagacgaa gacgtctcaa ttattcaagg tatgattgac
    17581 ttaatctttg ttaaagatgg tgtgcattat tttgtagact ataaaaccga tgcatttaat
    17641 cgtcgccgtg ggatgacaga tgaagaaatt ggtacacaat taaaaaataa atataagata
    17701 cagatgaaat attatcaaaa tacgcttcaa acgattctta ataaagaagt taaaggttat
    17761 ttatacttct tcaaatttgg tacattgcaa ctgtagtatt ttgattttca aaagaataaa
    17821 aaataatttc gattaagtgc aaagtccttg tagcagaatg aacacaactc attttcaaaa
    17881 ttgtcttact tatttatttg ttatttgata acgaaaaaag ttataatgtg aattaagata
    17941 aagatgagga gttgagaatg aatgaaattc ttatcattca agtataatga caaaacttca
    18001 tatggcgtta aagtaaaacg cgaagatgct gtatgggatt taacacaagt atttgctgac
    18061 tttgcagaag gagatttcca tcctaaaaca ttgttagctg gtttacaaca aaatcatact
    18121 ttagattttc aagaacaagt acgtaaagca gttgtagcag cagaagatag cggcaaagct
    18181 gaagactata aaatttcatt taatgacatt gaattcttac caccagtaac acctccgaat
    18241 aatgtgattg cttttggtag aaattacaaa gatcatgcga acgaattaaa tcatgaagta
    18301 gaaagattat atgtatttac aaaagcagcg tcatctttaa caggagataa tgcaaaaatt
    18361 ccaaatcata aagatattac tgatcaatta gattatgaag gtgaattagg tattgttatt
    18421 ggtaagtctg gtgaaaagat tccaaaagca ttagctttag attatgttta cggctataca
    18481 attattaacg atatcactga tcgcaaagca caaagtgaac aagatcaagc atttttatca
    18541 aaaagtttaa ctggcggttg cccaatgggt ccttatatcg ttactaaaga cgaactacca
    18601 ttacctgaaa atgtaaatat tgttacaaaa gttaacaacg aaattagaca agatggcaac
    18661 actggcgaaa tgattcttaa aattgatgaa ttaatagaag aaatttcaaa atatgttgca
    18721 ctacatccgg gagatattat tgcaactggt acaccagcag gcgttggtgc aggtatgcaa
    18781 ccaccaaaat tcttacaacc aggtgatgaa gttaaagtga ctattgataa tattggaacg
    18841 ctgacaactt atatcgctaa ataattatca tttaaaaagc taaccagtct ttatatagat
    18901 tggttagttt tttcttgctt ttctaaaaag tgttaaagat cattgtttat aatgttacca
    18961 ttttgagacg aaagtgaaat attgatatta agaagtagtt gattatttta cagtagatgc
    19021 acagtattct aataagtaat aaaatgtcat gttcttcctc taaaatatag aagtgtggta
    19081 aaatatatat ttgtgtatga acaaataatg attaaattat aagaaagtgg gtatttatcc
    19141 cacgaagttt taatttaata actaaaatta ttaaagtggg ggaattaata tgttacattt
    19201 acatatttta agttgggtat tagcaatcat tttatttatc gctacatact taaatatttc
    19261 aaaaaatcaa ggtggtacgc catatttcaa accattacat atggtgctac gattatttat
    19321 gctgttgacg ttaatttcag gattttggat attaattcag tcatttatga atggcggggc
    19381 aaatcatatg ttgcttacat tgaaaatgct gtgtggtgta gcagtagttg gattgatgga
    19441 agtgtcgatt gctaaaagaa agagacatga acaaagtcac acaatgtttt ggataacaat
    19501 tgcattaatt atcatcacaa tggtattagg tgtcattcta ccgttagggc ctatatcaaa
    19561 attattcggt attggctaat tgataagaca tatagtatat gacataaggc atggacaatt
    19621 attagtccaa gaccttatgt cttttttgat tctaaaaatg tttaaacaaa aaaaa
//
LOCUS       DATTQF010000035        19321 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.035, whole genome shotgun sequence.
ACCESSION   DATTQF010000035 DATTQF010000000
VERSION     DATTQF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.035"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            279..479
                     /locus_tag="VB241_001104"
     CDS             279..479
                     /locus_tag="VB241_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP1244407.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(606..1175)
                     /locus_tag="VB241_001105"
     CDS             complement(606..1175)
                     /locus_tag="VB241_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1244408.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCDLFNVTLDDLVKGTIPFAPDIKAQRSLNLWTYVMLIFMTLAAILTGPLVVY
                     WNWAWGITVIVIYGIGYYASMKIEDFKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNI
                     KTGVLSFIAFVGAIILIALISMHLANKFL"
     gene            complement(1428..1823)
                     /locus_tag="VB241_001106"
     CDS             complement(1428..1823)
                     /locus_tag="VB241_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEP1244409.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(1891..2244)
                     /locus_tag="VB241_001107"
     CDS             complement(1891..2244)
                     /locus_tag="VB241_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEP1244410.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2739..3578)
                     /gene="noc"
                     /locus_tag="VB241_001108"
     CDS             complement(2739..3578)
                     /gene="noc"
                     /locus_tag="VB241_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HEP1244411.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEENRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFEQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3621..4340)
                     /gene="rsmG"
                     /locus_tag="VB241_001109"
     CDS             complement(3621..4340)
                     /gene="rsmG"
                     /locus_tag="VB241_001109"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP1244412.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNYLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4340..6217)
                     /gene="mnmG"
                     /locus_tag="VB241_001110"
     CDS             complement(4340..6217)
                     /gene="mnmG"
                     /locus_tag="VB241_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP1244413.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6284..7663)
                     /gene="mnmE"
                     /locus_tag="VB241_001111"
     CDS             complement(6284..7663)
                     /gene="mnmE"
                     /locus_tag="VB241_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP1244414.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7807..8160)
                     /gene="rnpA"
                     /locus_tag="VB241_001112"
     CDS             complement(7807..8160)
                     /gene="rnpA"
                     /locus_tag="VB241_001112"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP1244415.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8281..8418)
                     /gene="rpmH"
                     /locus_tag="VB241_001113"
     CDS             complement(8281..8418)
                     /gene="rpmH"
                     /locus_tag="VB241_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP1244416.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            9149..10510
                     /gene="dnaA"
                     /locus_tag="VB241_001114"
     CDS             9149..10510
                     /gene="dnaA"
                     /locus_tag="VB241_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP1244417.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKEATKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10788..11921
                     /gene="dnaN"
                     /locus_tag="VB241_001115"
     CDS             10788..11921
                     /gene="dnaN"
                     /locus_tag="VB241_001115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP1244418.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            12302..12547
                     /gene="yaaA"
                     /locus_tag="VB241_001116"
     CDS             12302..12547
                     /gene="yaaA"
                     /locus_tag="VB241_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP1244419.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            12544..13656
                     /gene="recF"
                     /locus_tag="VB241_001117"
     CDS             12544..13656
                     /gene="recF"
                     /locus_tag="VB241_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP1244420.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            13666..15600
                     /gene="gyrB"
                     /locus_tag="VB241_001118"
     CDS             13666..15600
                     /gene="gyrB"
                     /locus_tag="VB241_001118"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP1244421.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEDKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            15637..18297
                     /gene="gyrA"
                     /locus_tag="VB241_001119"
     CDS             15637..18297
                     /gene="gyrA"
                     /locus_tag="VB241_001119"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP1244422.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESETDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEIISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDADEENEDEQSTVSEDGTEQQREAVVNDETPGNAIHTEVIDSEV
                     NDEDGRIEVRQDFMDRVEEDIQQSSDDDEE"
     gene            complement(18385..19197)
                     /locus_tag="VB241_001120"
     CDS             complement(18385..19197)
                     /locus_tag="VB241_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEP1244423.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         6452 a         3357 c         3217 g         6295 t
ORIGIN      
        1 ataaaaatgg tatcagtatt tagaccgata tatcaatata attgataaat tttcaaatgc
       61 ttataaaccc ctttaaatca acgtttctaa ggtttttaaa aatcttttta aatttatttt
      121 caaaaaacac tgtacattat gccaatatga gcgtatagtt ggacttacgt aataaaagct
      181 cgtgaattaa attgtagtgt atttgtttag aatatcctct tttttagtta tgaatttgtt
      241 acaaatatta agtgcaaaag cacacggagg ttttctatat gaataacggt acagtaaaat
      301 ggtttaacgc agaaaaaggt tttggtttca tcgaacaaga aaatggcgga gacgtattcg
      361 tacatttctc aggtatcgct agcgatggct acaaaacttt agaagaaggt caaaaagtta
      421 ctttcgaaat cactgaaggt caacgtggag accaagcagt taacgtacaa actgtttaat
      481 cttacaaaat aaaacgactc attataaatg aatcgctttg attaccaaca aggttctaaa
      541 tcttgttggt atttttttat gctattttag ataatataaa taatatgagg ccataccacg
      601 atgatttata aaaacttatt tgccagatgc atactaatca aagctataag aatgattgca
      661 ccaacaaatg ctatgaaaga aagtacacct gtttttatat ttctagcttt agtcgtttgt
      721 acttcactag gatcttttcc attcataaaa gcaacaattc gatcgtagtt atccatttta
      781 tgcacttttt taaagtcttc tattttcata gatgcataat aacctattcc ataaatgact
      841 atcaccgtta taccccaagc ccaattccaa taaacaacta aaggtcctgt taaaattgca
      901 gctagtgtca tgaaaataag catcacatat gtccataagt taagacttcg ttgcgcttta
      961 atatcaggtg caaatggaat ggtccctttt actaaatcat ctaaagttac attgaacaaa
     1021 tcacacatca ttaataagtt atgtatgtct ggtaagcttt tgtcattttc ccaattagaa
     1081 atactctgcc tagatacata taacttttca gcaagatatt cttgtgaata accatctcgt
     1141 tcccgatact ttttaatttg tttactcaga ttcaattaca tacgcctcca ttcaattagt
     1201 acaatcatta caatcagact atcagataaa gttaaatcaa gtcacctata tgattgacta
     1261 aatgttttga catactacaa catcagggtt cgaagactcc cttaagacag acctgtatga
     1321 ttacgcgctt gtaattttct caatgccaac ttatttatta atgatgcaac ttttccatgc
     1381 tattcaatgc ttgcgaagtt agtcctattt aagagattac gttcacacta tttactattc
     1441 gattttccag ctttccaact atgcttttct aatctttttt gtgctataaa ttctaccgct
     1501 tcaaaaatac atactgcact gatgaaccag cttaggaaac caacaataat gctaatcaac
     1561 cacatgtgcg tcatatgtaa catcatccat gtaactaaaa tacaaactaa tacccctgtc
     1621 ataccaaaca ctgcgccacg acttacatgc acagcgactt tatcaccata ttgcatacct
     1681 gtaataaaca tagacactaa aaatactgcc ggaaacgttg caaatatacc gccaaattct
     1741 ttccaaggta gtgttacaga tacaatataa cttaataata ctgcaaatcc ccccacaaaa
     1801 aatttcatta atgttaattt catgatgttc tgcctccaat aatttattac ttaataactg
     1861 aatacaacta attatcacgc attttccaaa ctataaatgt ctaatactga atattgcgag
     1921 ggaaataaca aaccaacaaa caactgtgaa tattgcgcct ttccgataac ctttatgctt
     1981 aatatagaga gatgtcaaaa atacagttaa tatacaagac agaataccga caattgctcc
     2041 agtactaaga ttcatcgaca tctccactaa ttgcgtacca cgatgatcca aagctaatgc
     2101 gataatagcc gcaagaaaga ctgccggcat agtagctata atacctccta acttaccacc
     2161 taccttatca gcaataattg aagctaatgc aacagcgata ccaccaatga caaaatgaag
     2221 aattgcactt ccaattgaaa acatcttcaa cgtacctttc tataattaat tcaatacaaa
     2281 acgtatcatt cacacaaagt gtaaaactta aattttctac tgtaaaggca actcaagcga
     2341 tgttcataaa gctttcatga tactttcata aactagcaat aaagggttca aataaaggta
     2401 tatccagctc aaattaggga gtgggacaga aatgatattt tcgcaaaatt tatttcgtcg
     2461 tcccacccca actcgcattg cctgtagaat ttcttttcga aattctctgt gttggggccc
     2521 ctgactttaa ttgaaaaaag cttgttacaa gcgcattttc gttcagtcaa ctaccgccaa
     2581 tataacttcg tagagcatag aacattgatt tatgtcccag cctgatcaac atataaatat
     2641 aaatttttat gtttcacgta aaacaaaaaa agacaaagct gtaatgatct tagctttgtc
     2701 ttttatgtgt tagtcatcat gtatacgaca tcctactact aacgtttata tattcgaatt
     2761 tttatttcat aataatcttc atgatctttg tctttatgct caacatgtaa tcctgtttgt
     2821 tgaatcgctt ggatactttt gcctacctca tctcgtgctt gcgtgacatc ttgttcaaag
     2881 cgaaggtttt gtgctttgac tttttcgggc ccagtttttt ggcgtactct atcttcagtc
     2941 tgtttcacat ttagtttttg tgcaatgact tgctcaatca acgcttcttg ttcgctatca
     3001 gacaatgata ataccgctct cgcatggcgt tcagtaattt taccttctct taagcgaagt
     3061 agtactttcg gcgccaactt caataaacgc aacttatttg caataaagct ttgactttta
     3121 cctaaacttt ttgccaattc actttgcgtt gtatcaccaa tttccaataa tttcttatag
     3181 gcttccgctt cttcaacaac agacaaattt tctctttgaa tattctcaat taatgccaca
     3241 acagccgtct cttcatcatc catatcacga ataataacgt ctgcttgagg taaatttagt
     3301 gattgtattg ctctaaatcg gcgctctcca gcaataattt caaacatatc ttcttcaatc
     3361 ggtcttacaa caataggctg taataaacca tgttcatgta ttgattcagc aagttcttta
     3421 attttatttg gttcaaatac ctgtcttggt tgataacggt tgggaacgat acgttcaatt
     3481 tgaatggatt caacattact attgcgattt tcttcaatat gtccaatgat gtcatctttg
     3541 tttttcaaac caaataattt tgaaaaaggt tttttcatta tccattcgct ccttttatgt
     3601 tagttgtaaa ggattatgca ttatttttca agtaaaggag tcttattagg cgtccctggt
     3661 tttcttggat actttttcgg cgtctgtctt tttttatcaa taatgaacat ctggcgctct
     3721 ccagcatctt ctggcaattc aaaggtatgt gtttctgtaa cgttaccacc taacacacta
     3781 attgcaaatt ttgcttcttc taattcttct tcaccttttg aagattttaa tgcaacaaac
     3841 tgtccacctt ttttaactag cggtaaacac aattcactca acacggataa tctagctact
     3901 gctcttgcag taacaacatc ataagactcc ctgtagacac ccttaccaaa tgtttctgct
     3961 ctatcgtgta taaagctgac atcctgtaat tgtaattctg acgctaaata gtttaaaaat
     4021 tgaatacgct tatttaatga atcaacaatc gtcactttta actgcggaaa cattattttt
     4081 aacggaatac ttggaaaacc agctccagcg cctacatcac atatacttat aggctgatta
     4141 aaatcaaaat aaaaactagg tgcaatggaa tcataaaaat gtttcaaata tacatcgtgt
     4201 tcatctgtaa tacttgtcaa attcatcttt tcattccatt caacaagtaa acgataatat
     4261 gtttgaaact gttgtttttg agtctcagtt aattcaatat tatgttcttt taattgttct
     4321 gctaaccatt ctacagtcat tagtcactca ccctttggag tttaccttgt tctaagtaaa
     4381 tcaataatat agatatgtct gctggattta cccctgatat tctagaagct tgtgcaatat
     4441 ttaaaggttt tacttctgat aatttttctc gcgcttcagt cgccaaacta tcaatcttac
     4501 tataatctaa gtcttctgga attttcttct cttccatacg tttaactttc tcaacttgtt
     4561 gtagtgattt attgatataa ccttcatatt ttgtttgtat ttctacttgt tcttcaacat
     4621 ctgcattcaa ttgatgttct tcttctaaaa tttctaaaat tatatcgtaa gtcatttcag
     4681 gtctgcgtaa taaatcgata gctaaaatac catcttttaa gcgagaacca ccatgttgtt
     4741 caataatcgc ttgcgtatgt tcgtttggtt taatacgaat atctgataaa cgcttaattt
     4801 ccgcatcaat ttgctgacgt ttttcattaa aacgtgcata tcgttcttca gaaatcatac
     4861 caagttcata tcccatatcc gtcaatctca aatcagcatt atcatgacgt agtaacaaac
     4921 gatattctgc acgtgatgtt agtaaacgat aaggttcatt agtaccttta gttacaagat
     4981 catcgattaa tacacctata tatgcatctg aacgacttaa tatcttttcg cctgtgttta
     5041 acactttacc tgcagcgtta atacctgcca tcaatccttg tcctgctgct tcttcataac
     5101 cagatgtacc attaatttga cctgcagtgt ataggttttt aatcattttc gtttcaagtg
     5161 taggccataa ctgcgttggc acaatcgcat catattcaat tgcgtagccg gcacgcatca
     5221 tatctgcttt ttcaagacct ggtatcgtct ctaacatttg acgttgcaca tgttcaggaa
     5281 gacttgtaga caatccttgc acatatactt catttgtatt acgaccttca ggctctaaga
     5341 aaagttgatg tcgcggctta tcattaaatc gaacaaattt atcttcaatt gaagggcaat
     5401 aacgtggccc ggttccttta atcatccctg aatacattgc agatagatgt aaattatcat
     5461 cgataacttt gtgtgtttca gcattagtat acgttagcca acatggcaat tgatctaata
     5521 tatattctgt tgtttcaaag ctgaatgcac gacccacatc gtcacctggt tgtatttcag
     5581 tcttcgaata gtcaattgtt tttgaattta cacgcggtgg tgtacctgtt ttaaaacgaa
     5641 caatatcaaa accaagttct cttaaattgt ctgataatgt gattgatggt aattggtgat
     5701 ttggtccact tgaatacttc atattaccta aaatgatttc accacgtaaa aatgttcccg
     5761 ttgtaataat tactgcttta gataaatact ctgtaccaat atttgtacgt acacctttaa
     5821 cttcattatc ttctataata agttcgtcta ccataccttg cattatatgc aaattttctt
     5881 catcttcaat cacgcgtttc atttcttgtt gataaagtac tttatctgct tgcgctctta
     5941 gtgctcttac agcaggtcct ttaccagtat ttaacattct catttgaatg tgtgttttat
     6001 cgattgtttt tgccatttgt ccacctaaag catcaatttc acgaacaacg atacctttag
     6061 ctggtccacc tacagatggg ttacatggca taaatgcaat attatctaaa tttattgtta
     6121 gcattaatgt tttagcacca cgtcttgcag atgctaaacc tgcttctaca cctgcatgtc
     6181 ccgcacctat aacgattaca tcatattctt gaaccacaat ataaacctcc ttatttgata
     6241 tcttactagc cttcttaaga cggtattccg tctatttcaa ttactattta cctaagcaga
     6301 attgactgaa taactgatcg atgagttcat cacttgcagt ctcaccaata atttctccta
     6361 atatttccca agttctagtt aaatcgattt gtaccatatc cataggcaca ccagattctg
     6421 ctgcatcaat cgcatcttgt atcgtttgtc ttgcttgttt taataatgaa atatgtcttg
     6481 aattagaaac ataagtcata tcttgatttt gtacttctcc accaaagaac aaatctcgaa
     6541 tttgtatttc taattcatca ataccttctt gttttaacat tgaagtttga attaatggcg
     6601 tatcgcctat catatcttta acttcattaa tatctatatt ttgctctaaa tccattttat
     6661 taacaattac gattacatct tcatttttaa ccacttcata taatgtgtaa tcttcttgag
     6721 tcaatgcttc gttattgttt aatacaaata aaattaagtc cgcttggcta agagcctttc
     6781 tagagcgttc aacaccaatc ttctctacta tatcttctgt ctcacgtata ccagcagtat
     6841 caactaatct taatggcacg ccacgaacat tgacgtactc ttctaagaca tctctagtcg
     6901 tacctgctac ctcagttaca atcgctttat tatcttgtat taaattattt aacatcgatg
     6961 atttacctac atttggttta ccaacaataa ctgtagataa accttcacgc ataattttac
     7021 cctgcgcacc ggtatctaat aaacgattaa tttcctgttt gatttcttta gactgctcta
     7081 aaagaaattc agtagtcgca tcttcaacat catcgtattc aggataatca atattcactt
     7141 ccacttgagc gagtatctct aatatagatt gacgttgttt tttgattaag tcacttagac
     7201 gaccttcaat ttgattcatc gcaactttag aagctctatc tgttttcgag cgaataaagt
     7261 ccataactgc ctcagcttga gataaatcaa tacgaccatt taaaaaggca cgttttgtaa
     7321 attcacctgg ttcagccatt ctagcgccat atgtcatagt aagttccagc actctattaa
     7381 tcgttaaaat accaccatga caattaattt ctataatatc ttcgcgtgta aatgtttttg
     7441 gtgctcttaa cacagacacc ataacttctt caaccacttc tttagactct ggatcaataa
     7501 tatgaccgta attaatcgta tgtgatggaa catcatttaa aagatgtttt cctttatata
     7561 atttgtcagc aatttcaacg gcttgcggtc cagacaatcg aacaattcca attgcccctt
     7621 cacccattgg tgttgaaata ctcgtaattg tatctaaatc catattgcta ctcgcctcct
     7681 tcaacgatgt gaatacattt taaagtaaat tattataact ctaaggtaag tcttacgttt
     7741 gtctgaggta agactccggg gtgtgttgag tggttaatgt tttcctctct ttcccctacc
     7801 ctatccttac ttaatctttt tattaaaaac tttggcaatt ttaagtacgt gctcaagact
     7861 attctgtatt tgtaaagtgg tcatatcttt agctggctgt cttgctatta caataatatc
     7921 tttggccaat atatgcgact tatgtacttt gaaattttca cgtattgctc ttttaatctt
     7981 gtttcttaac actgcattac ctagtttttt agaaacacta atacctaagc gaaaatggtc
     8041 tatttcttta ttattacaag tgtatacaac aaattgtctg ttggctacag aatgaccttt
     8101 tttatatatt ctctgaaaat ctgcattctt tttaattcgg taagcttttt ccaataacat
     8161 cactcgctta tttatcgttt ttatttgaag ctatatttaa acttctattg agcttataac
     8221 ataaatttct atttattctt aatttaaacg aaaaaaaaga tcactgatga gtcagtgatc
     8281 ttatgcagat aaaactttac ggcctttacg acgacggcgc gctaaaactt tacggccatt
     8341 ttttgtgctc atgcgttttc tgaaaccatg aactttacta tgtttacgtt tatttggttg
     8401 ataagtacgt tttaccatgc aaaacacctc catctttata cagttatcta atataccaga
     8461 ttatgcagac tctgatcact ttaatagata agattttgcg tttcttagat taaaattctc
     8521 gtattagctc attgattatc tagtcataat tcaagcaact actacaatat aacaaaatcc
     8581 tttttataac gcaagttcat tttatactac tgctcaattt ttttactttt atcgattaaa
     8641 gatagaaata cacgatgcga gcaatcaaat ttcataacat caccatgagt ttgatccaaa
     8701 gcatgagtgt ttacaatgtt tgaatacctt atacagttct tatacatact ttataaatta
     8761 tttcccaagc tgttttgata cacacactaa cagatactct atagaaggaa aagttatcca
     8821 cttatgcaca cttatacttt ttagaattgt ggataattag aaattacaca caaagttata
     8881 ctatttttag caacatattc acaggtattt gacatataga gaactgaaaa agtataattg
     8941 tgtggataag tcgtccaact catgatttta taaggattta tttattgata tttacataaa
     9001 aatactgtgc ataactaata agcaggataa agttatccac cgattgttat taacttgtgg
     9061 ataattatta acatggtgtg tttagaagtt atccacggtt gttatttttg tgtataactt
     9121 aaaaatttaa gaaagatgga gtaaatttat gtcggaaaaa gaaatttggg aaaaagtgct
     9181 tgaaattgct caagaaaaat tatcagctgt aagttactca actttcctaa aagatactga
     9241 gctttacacg atcaaagatg gtgaagctat cgtattatcg agtattcctt ttaatgcaaa
     9301 ttggttaaat caacaatatg ctgaaattat ccaagcaatc ttatttgatg ttgtaggcta
     9361 tgaagtaaaa cctcacttta ttactactga agaattagca aattatagta ataatgaaac
     9421 tgctactcca aaagaagcaa caaaaccttc tactgaaaca actgaggata atcatgtgct
     9481 tggtagagag caattcaatg cccataacac atttgacact tttgtaatcg gacctggtaa
     9541 ccgcttccca catgcagcga gtttagctgt ggccgaagca ccagccaaag cgtacaatcc
     9601 attatttatc tatggaggtg ttggtttagg aaaaacccat ttaatgcatg ccattggtca
     9661 tcatgtttta gataataatc cagatgccaa agtgatttac acatcaagtg aaaaattcac
     9721 aaatgaattt attaaatcaa ttcgtgataa cgaaggtgaa gctttcagag aaagatatcg
     9781 taatatcgac gtcttattaa tcgatgatat tcagttcata caaaataaag tacaaacaca
     9841 agaagaattt ttctatactt ttaatgaatt gcatcagaat aacaagcaaa tagttatttc
     9901 gagtgatcga ccgccaaagg aaattgcaca attagaagat cgattacgtt cgcgctttga
     9961 atgggggcta attgttgata ttacgccacc agattatgaa actcgaatgg caattttgca
    10021 gaagaaaatt gaagaagaaa aattagatat tccaccagaa gctttaaatt atatagcaaa
    10081 tcaaattcaa tctaatattc gtgaattaga aggtgcatta acacgtttac ttgcatattc
    10141 acaattatta ggaaaaccaa ttacaactga attaactgct gaagctttaa aagatatcat
    10201 tcaagcacca aaatctaaaa agattaccat ccaagatatt caaaaaattg taggccagta
    10261 ctataatgtt agaattgaag atttcagtgc aaaaaaacgt acaaagtcaa ttgcatatcc
    10321 acgtcaaata gctatgtact tgtctagaga gcttacagat ttctcattac ctaaaattgg
    10381 tgaagaattt ggtgggcgtg atcatacgac cgtcattcat gctcatgaaa aaatatctaa
    10441 agatttaaaa gaagatccta tttttaaaca agaagtagag aatcttgaaa aagaaataag
    10501 aaatgtataa gtaggaaact ttgggaaatg taatctgtta tataacagta ctaataataa
    10561 caatcatttt ttacatttct atatgctaat gtggcaagat gagcaaaact cattttgtgg
    10621 ataatgttta aaattcatac acgccataca caagttatca acatgtgtat aacttcgcca
    10681 aatctatgtt tttaagactt atccaccaat ccacagcacc tactactatt actaagaact
    10741 taaaacctat ataattatat ataaacgact ggaaggagtt ttaattaatg atggaattca
    10801 ctattaaaag agattatttt attacacaat taaatgacac attaaaagct atttcaccaa
    10861 gaacaacatt acctatatta actggtatca aaatcgatgc aaaagaacat gaagttatat
    10921 taactggttc agactctgaa atttcaatag aaatcactat tcctaaaact gtagatggcg
    10981 aagatattgt caatatttca gaaacaggct cagtagtact tcctggacga ttctttgttg
    11041 atattataaa aaaattacct ggtaaagatg ttaaattatc tacaaatgaa caattccaga
    11101 cattaattac atcaggtcat tctgaattta atttaagtgg cttagatcca gatcaatatc
    11161 ctttattacc tcaagtttct agagatgacg caattcaatt gtcggtaaaa gtgcttaaaa
    11221 acgtaattgc acaaacgaat tttgcagtgt ccacctcaga aacacgccca gtactaactg
    11281 gtgtgaactg gcttatacaa gaaaatgaat taatatgcac agcgactgac tcacaccgct
    11341 tggctgtaag aaagttgcag ttagaagatg tttctgaaaa caaaaatgtc atcattccag
    11401 gtaaggcttt agctgaatta aataaaatta tgtctgacaa tgaagaagac attgatatct
    11461 tctttgcttc aaaccaagtt ttatttaaag ttggaaatgt gaactttatt tctcgattat
    11521 tagaaggaca ttatcctgat acaacacgtt tattccctga aaactatgaa attaaattaa
    11581 gtatagacaa tggggagttt tatcatgcga ttgatcgtgc atctttatta gcacgtgaag
    11641 gtggtaataa cgttattaaa ttaagtacag gtgatgacgt tgttgaattg tcttctacat
    11701 caccagaaat tggtactgta aaagaagaag ttgatgcaaa cgatgttgaa ggtggtagcc
    11761 tgaaaatttc attcaactct aaatatatga tggatgcttt aaaagcaatc gataatgatg
    11821 aggttgaagt tgaattcttc ggtacaatga aaccatttat tctaaaacca aaaggtgacg
    11881 actcggtaac gcaattaatt ttaccaatca gaacttacta aaaataaata taaataaagg
    11941 atgacgtgat taattaaaac gtcatccttt attttttggc aaaaataatt ctagattcgt
    12001 atgtaaaata aatttgacag cattttaaac agcaaataaa agacgccaat taaatttatg
    12061 acaaatgtat ccagaattta ataagtgtgc ttatatgccc tttaaattca aaattttaat
    12121 agtcaataac aagttgaata taaaagttaa acgccgttaa atagcgttaa aaaattgaaa
    12181 atgacagtat tgccaaaaaa taagaattaa ttatttatat gtaaacggtt tccacctcta
    12241 ttttaaatga aatttgtgac aaaaaaaggt ataatatatt aatgacatac aaagaaatgg
    12301 agtgattatt ttggttcaag aagttgtagt agaaggagac attaatttag gtcaatttct
    12361 aaaaacagaa gggattattg aatctggtgg tcaagcaaaa tggttcttgc aagacgttga
    12421 agtattaatt aatggagtgc gtgaaacacg tcgcggtaaa aagttagaac atcaagatcg
    12481 tatagatatc ccagaattac ctgaagatgc tggttctttc ttaatcattc atcaaggtga
    12541 acaatgaagt taaatacact ccaattagaa aattatcgta actatgatga ggttacgttg
    12601 aaatgtcatc ctgacgtgaa tatcctcatt ggagaaaatg cacaaggaaa gacaaattta
    12661 cttgaatcaa tttatacctt agctttagca aaaagtcata gaacgagtaa tgataaggaa
    12721 ctcatacgtt ttaatgctga ttatgctaaa atagaaggtg agcttagtta tagacacggc
    12781 acgatgccat taacaatgtt tataactaaa aaaggtaaac aagtcaaagt gaatcactta
    12841 gagcaaagtc gtctaactca atatattgga cacctcaatg tggttctatt tgcgccagaa
    12901 gatttgaata ttgtaaaagg ctctcctcaa ataagacgac gctttataga tatggagttg
    12961 ggccaaattt ctgctgttta cttaaatgat ttagctcaat accaacgtat tttaaagcaa
    13021 aagaataatt acttaaagca gttacaatta ggccaaaaaa aggacttaac aatgttggaa
    13081 gtattaaatc aacagtttgc tgaatatgca atgaaagtaa ctgataaacg tgcacatttt
    13141 attcaagagc tagagtcgtt agctaaaccg attcatgctg gtatcacaaa tgataaagaa
    13201 gcgttgtcgc tgaattattt acctagtctt aaatttgatt atgctcaaaa tgaagcggca
    13261 cgacttgaag aaattatgtc tattcttagc gataatatgc aaagagaaaa agaacgaggc
    13321 attagcttat tcggaccaca tcgagatgat ataagttttg atgtgaatgg catggatgct
    13381 caaacatatg gttctcaagg acagcaacgt acaacggctt tgtccattaa attagctgaa
    13441 attgagttaa tgaatatcga agttggggaa tatcccatct tattattaga cgatgtactc
    13501 agtgaattag atgattcgcg tcaaacgcat ttattaagta cgattcagca taaagtacaa
    13561 acatttgtca ctacgacatc tgtagatggt attgatcatg aaatcatgaa taacgctaaa
    13621 ttgtatcgta ttaatcaagg tgaaattata aagtaacaga aagcgatggt gactgcattg
    13681 tcagatgtaa acaacacgga taattatggt gctgggcaaa tacaagtatt agaaggttta
    13741 gaagcagtac gtaaaagacc aggtatgtat ataggatcga cttcagagag aggtttgcac
    13801 catttagtgt gggaaattgt cgataatagt atcgatgaag cattagctgg ttatgcaaat
    13861 caaattgaag ttgttattga aaaagataac tggattaaag taacggataa cggacgtggt
    13921 atcccagttg atattcaaga aaaaatggga cgtccagctg tcgaagttat tttaactgtt
    13981 ttacatgctg gtggtaaatt cggcggtggc ggatacaaag tatctggtgg tttacatggt
    14041 gttggttcat cagttgtaaa cgcattgtca caagacttag aagtatatgt acacagaaat
    14101 gagactatat atcatcaagc atataaaaaa ggtgtacctc aatttgactt aaaagaagtt
    14161 ggcacaactg ataagacagg tactgtcatt cgttttaaag cagatggaga aatcttcaca
    14221 gagacaactg tatacaacta tgaaacatta cagcaacgta ttagagagct tgctttctta
    14281 aacaaaggaa ttcaaatcac attaagagat gaacgtgatg aagaaaacgt tagagaagac
    14341 tcctatcact atgagggcgg tattaaatct tatgttgagt tattgaacga aaataaagaa
    14401 cctattcatg atgagccgat ttatattcat caatctaaag atgatattga agtagaaatt
    14461 gcgattcaat ataactcagg atatgccaca aatcttttaa cttacgcaaa taacattcat
    14521 acgtacgaag gtggtacgca tgaagacgga ttcaaacgtg cattaacgcg tgtcttaaat
    14581 agttatggtt taagtagcaa gattatgaaa gaagacaaag atagactttc tggtgaagat
    14641 acacgtgaag gtatgacagc aattatatct atcaaacatg gtgatcctca attcgaaggt
    14701 caaacgaaga caaaattagg taattctgaa gtgcgtcaag ttgtagataa attattctca
    14761 gagcactttg aacgattttt atatgaaaat ccacaagtcg cacgtacagt ggttgaaaaa
    14821 ggtattatgg cggcacgtgc acgtgttgct gcgaaaaaag cgcgtgaagt aacacgtcgt
    14881 aaatcagcgt tagatgtagc aagtcttcca ggtaaattag ccgattgctc tagtaaaagt
    14941 cctgaagaat gtgagatttt cttagtcgaa ggggactctg ccggggggtc tacaaaatct
    15001 ggtcgtgact ctagaacgca ggcgatttta ccattacgag gtaagatatt aaatgttgaa
    15061 aaggcacgat tagatagaat tttgaataac aatgaaattc gtcaaatgat cacagcattt
    15121 ggtacaggaa tcggtggcga ctttgatcta gcgaaagcaa gatatcacaa aatcgtcatt
    15181 atgactgatg ccgatgtgga tggagcgcat attagaacat tgttattaac attcttctat
    15241 cgatttatga gaccgttaat tgaagcaggc tatgtgtata ttgcacagcc accgttgtat
    15301 aaactgacac aaggtaaaca aaagtattat gtatacaatg atagggaact tgataaactt
    15361 aaatctgaat tgaatccaac accaaaatgg tctattgcac gatacaaagg tcttggagaa
    15421 atgaatgcag atcaattatg ggaaacaaca atgaaccctg agcaccgcgc tcttttacaa
    15481 gtaaaacttg aagatgcgat tgaagcggac caaacatttg aaatgttaat gggtgacgtt
    15541 gtagaaaacc gtagacaatt tatagaagat aatgcagttt atgcaaactt agacttctaa
    15601 gcgctgtgaa ctgaactttt gaaggaggaa ctcttgatgg ctgaattacc tcaatcaaga
    15661 ataaatgaac gaaatattac cagtgaaatg cgtgaatcat ttttagatta tgcgatgagt
    15721 gttatcgttg ctcgtgcatt gccagatgtt cgtgacggtt taaaaccagt acatcgtcgt
    15781 atactatatg gattaaatga acaaggtatg acaccggata aatcatataa aaaatcagca
    15841 cgtatcgttg gtgacgtaat gggtaaatat caccctcatg gtgactcatc tatctatgaa
    15901 gcaatggtac gtatggctca agatttcagt tatcgttatc cgcttgttga tggccaaggt
    15961 aactttggtt caatggatgg agatggcgca gcagcaatgc gttatactga agcacgtatg
    16021 actaaaatca cacttgaact gttacgtgat attaataaag atacaataga ttttatcgat
    16081 aactatgatg gtaatgaaag agagccgtca gtcttacctg ctcgattccc taacttgtta
    16141 gccaatggtg catcaggtat cgcggtaggt atggcaacga atattccacc acataactta
    16201 acagaattga tcaatggtgt acttagctta agtaagaatc ctgatatttc aattgctgag
    16261 ttaatggaag atattgaagg tcctgatttc ccaactgctg gacttatttt aggtaagagt
    16321 ggtattagac gcgcatatga aacaggtcgt ggttcaattc aaatgcgttc tcgtgcagtt
    16381 attgaagaac gtggaggcgg acgtcaacgt attgttgtca ctgaaattcc tttccaagtg
    16441 aataaggctc gtatgattga aaaaattgca gagcttgttc gtgacaagaa aattgacggt
    16501 attactgatt tacgtgatga aacaagttta cgtactggtg tgcgtgtcgt tattgatgtg
    16561 cgtaaggatg ccaatgctag tgtcatttta aataacttat acaaacaaac acctcttcaa
    16621 acatcatttg gtgtgaatat gattgctcta gtgaatggta gaccgaagct gattaattta
    16681 aaagaagcgt tagtacatta tttagagcat caaaagacag ttgttagaag acgtacgcaa
    16741 tacaacttac gtaaagctaa agatcgtgcc cacattttag aagggttacg tattgcgtta
    16801 gatcatatcg atgaaattat ttcaacgatt cgtgaatcag aaacagataa agtcgcaatg
    16861 gaaagtttgc aacaacgctt caaactttct gaaaaacaag ctcaagctat tttagacatg
    16921 cgtttaagac gtctaacagg tttagagaga gacaaaattg aagctgaata taatgagtta
    16981 ttaaattata ttagtgaatt agaagcaatc ttagctgatg aagaagtgtt attacagtta
    17041 gttagagatg aattgactga aattagagat cgcttcggtg atgatcgtcg tactgaaatt
    17101 caattaggtg gatttgaaga cttagaggac gaggacttaa ttccagaaga acaaattgta
    17161 attactttga gccataataa ctatattaaa cgtttgccgg tatctacata tcgtgctcaa
    17221 aaccgtggtg gtcgtggtgt tcaaggtatg aatacattgg aagaagattt tgtcagtcaa
    17281 ttggtaactt taagtacaca tgaccatgta ttgttcttta ctaacaaagg tcgtgtatac
    17341 aaactaaaag gttatgaagt gcctgagtta tcaagacagt ctaaaggtat tcctgtagtg
    17401 aatgctattg aacttgaaaa tgatgaaatc attagtacaa tgattgctgt taaagacctt
    17461 gaaagtgaag ataacttctt agtgtttgca actaaacgtg gtgtcgttaa acgttcagca
    17521 ttaagtaact tctcaagaat aaatagaaat ggtaagattg cgatttcgtt cagagaagat
    17581 gatgagttaa ttgcagttcg cttaacaagt ggtcaagaag atatcttgat tggtacatca
    17641 catgcatcat taattcgatt ccctgaatca acattacgtc ctttaggccg tacagcaacg
    17701 ggtgtgaaag gtattacact tcgtgaaggt gacgaagttg tagggcttga tgtagctcat
    17761 gcaaacagtg ttgatgaagt attagtagtt actgaaaatg gttatggtaa acgtacgcca
    17821 gttaatgact atcgtttatc aaatcgtggt ggtaaaggta ttaaaacagc tacgattact
    17881 gagcgtaatg gtaatgttgt atgtatcact acagtaactg gtgaagaaga tttaatgatt
    17941 gttactaatg caggtgtcat tattcgacta gatgttgcag atatttctca aaatggtcgt
    18001 gcagcacaag gtgttcgctt aattcgctta ggcgatgatc aatttgtttc aacggttgct
    18061 aaagtaaaag aggatgcaga cgaagaaaat gaagatgaac aatctactgt atctgaagat
    18121 ggtactgaac aacaacgtga agcggttgta aatgatgaaa caccaggaaa tgcaattcat
    18181 actgaagtga ttgattcaga agtaaatgat gaagatggac gtattgaagt aagacaagat
    18241 ttcatggacc gtgttgaaga agacatacaa caatcatcag atgatgatga agaataataa
    18301 aaaaataaga cttccctata tgtagggggg tcttattttt atgctagaaa gtaatgctat
    18361 actatattca atgattagta atgactaact ttctaattgt ttcattgcgt aaggtatttc
    18421 attgataagt cttgatggtg gcaccacata catatctttt gcaagatttt cgccaataaa
    18481 actatgtgta tatgtggcac tcataaccgc ttcttttaag ttatcaaatt gaccaacaaa
    18541 acttgtaatc ataccagcaa gtgtatcgcc cataccacca gtcgccattg ctgggctacc
    18601 gattgtcaat ttaaagtctt catctttaaa gaaaatttca gtaccgtgtt ttttaagtac
    18661 aacagttgcg cctaaacgat caactgcttc acgattacgc tcatatgtct gttcctcaat
    18721 aggaatacca cttaatcgtt cccattcttt gagatgcgga gtaaaaatca cgcgacatgt
    18781 aggtaattgt ggtttcaatt tactaaagat tgtgatggca tcgccgtcta cgattaaatt
    18841 ttgatgcggt tgtatatttt gtagtaggaa tgtaatggca ttatttcctt tgaaatcaac
    18901 gccaagacct ggaccaatta gtatactgtc agtcatttca atcattttcg tcaacatttt
    18961 cgtatcatta atatcaataa ccatcgcttc tgggcaacga gaatgtaatg ctgaatgatt
    19021 tgttggatgt gtagctacag tgattaaacc actaccgcta aatacacatg cacgagccgc
    19081 taacataatg gcaccaccta agttagcaga tccaccaatt aataaaattt tgccataatc
    19141 acctttatgt gaatcttctt tacgcttagg aatgttaata gaatttaacg tttccatagt
    19201 gatataacct cccatgtaaa agccttttcc gaatttattc aattttaaaa atatatagta
    19261 acttttaaca aaatgtatta taaatttctg aattcattat tatttgtcgt taaatacaat
    19321 a
//
LOCUS       DATTQF010000036        19145 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.036, whole genome shotgun sequence.
ACCESSION   DATTQF010000036 DATTQF010000000
VERSION     DATTQF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.036"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            75..1370
                     /gene="purB"
                     /locus_tag="VB241_001121"
     CDS             75..1370
                     /gene="purB"
                     /locus_tag="VB241_001121"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP1244424.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1479..1781
                     /locus_tag="VB241_001122"
     CDS             1479..1781
                     /locus_tag="VB241_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244425.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            1953..2645
                     /locus_tag="VB241_001123"
     CDS             1953..2645
                     /locus_tag="VB241_001123"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HEP1244426.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHLYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2642..4834
                     /gene="pcrA"
                     /locus_tag="VB241_001124"
     CDS             2642..4834
                     /gene="pcrA"
                     /locus_tag="VB241_001124"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP1244427.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            4838..6841
                     /gene="ligA"
                     /locus_tag="VB241_001125"
     CDS             4838..6841
                     /gene="ligA"
                     /locus_tag="VB241_001125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP1244428.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEFKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVSDIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            6854..8053
                     /locus_tag="VB241_001126"
     CDS             6854..8053
                     /locus_tag="VB241_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HEP1244429.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8142..9680)
                     /gene="putP"
                     /locus_tag="VB241_001127"
     CDS             complement(8142..9680)
                     /gene="putP"
                     /locus_tag="VB241_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEP1244430.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLVRGEEKAKTHQKEFVMVGRLSVLAV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10047..10349
                     /gene="gatC"
                     /locus_tag="VB241_001128"
     CDS             10047..10349
                     /gene="gatC"
                     /locus_tag="VB241_001128"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP1244431.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10351..11808
                     /gene="gatA"
                     /locus_tag="VB241_001129"
     CDS             10351..11808
                     /gene="gatA"
                     /locus_tag="VB241_001129"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP1244432.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADANDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            11821..13248
                     /gene="gatB"
                     /locus_tag="VB241_001130"
     CDS             11821..13248
                     /gene="gatB"
                     /locus_tag="VB241_001130"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP1244433.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSEDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13287..13433
                     /locus_tag="VB241_001131"
     CDS             13287..13433
                     /locus_tag="VB241_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244434.1"
                     /translation="MVADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            14071..15018
                     /locus_tag="VB241_001132"
     CDS             14071..15018
                     /locus_tag="VB241_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP1244435.1"
                     /translation="MRKRARIIYNPTSGKEQFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            15099..16460
                     /gene="rlmD"
                     /locus_tag="VB241_001133"
     CDS             15099..16460
                     /gene="rlmD"
                     /locus_tag="VB241_001133"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP1244436.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDMG
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMVIFVTN
                     GKNWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPSTQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            16630..17160
                     /locus_tag="VB241_001134"
     CDS             16630..17160
                     /locus_tag="VB241_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HEP1244437.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSAGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            17407..18477
                     /gene="dinB"
                     /locus_tag="VB241_001135"
     CDS             17407..18477
                     /gene="dinB"
                     /locus_tag="VB241_001135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP1244438.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYRNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSTRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(18544..19098)
                     /locus_tag="VB241_001136"
     CDS             complement(18544..19098)
                     /locus_tag="VB241_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEP1244439.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
BASE COUNT         7060 a         2806 c         3577 g         5702 t
ORIGIN      
        1 actttttatt tatttttatt aaaaatgttc tgtttttgaa aattcgagta tacatacgat
       61 aggagttaaa aaaaatgatt gaacgttatt ctagagaaga aatgtctaat atttggactg
      121 atcaaaatcg ctatgaagca tggttagaag tggaaatttt agcatgtgag gcatggagtg
      181 aattaggaca tataccgaaa gctgacgtac aaaaaattcg tcaaaacgca aaggtaaatg
      241 tcgaacgtgc acaagaaatt gaacaagaaa cgcgccatga tgttgtagcc tttactagac
      301 aagtttctga aacgctaggt gaagaacgta agtgggtaca ttatggttta acttctactg
      361 atgttgtaga tacagcttta agtttcgtta ttaaacaagc aaatgacatt attgaaaaag
      421 atttagaaag atttatcgac gttttagctg aaaaagcaaa aaattataaa tatacattga
      481 tgatgggacg tacacatgga gtgcatgcag aaccaactac atttggtgtc aaaatggcat
      541 tgtggtacac ggaaatgcaa cgtaacttac aacgtttcaa acaagtaaga gaagaaattg
      601 aagtagggaa aatgagtggt gcagtaggta cttttgctaa cattcctcct gaaatagaaa
      661 gttatgtgtg taaacattta ggtattggca cagcgcctgt atcaacgcaa acattgcaac
      721 gcgatcgaca tgcatactat attgcgacat tagcattaat cgcaacttca ctagaaaaat
      781 ttgccgttga aattcgtaac cttcaaaaaa ccgaaacaag agaagttgaa gaagcatttg
      841 caaaaggtca aaaaggttca tctgcaatgc ctcataaacg aaatccaatt ggttctgaaa
      901 atatcacagg tatttcaaga gtaattcgtg gttatattac tacagcttat gagaatgtgc
      961 cattatggca tgaaagagat atttctcatt cttcagctga gcgtattatg ttaccagatg
     1021 ttacaatagc actagattat gcattgaatc gtttcacaaa tattgtggac cgtttaactg
     1081 tatttgaaga taatatgcgt aataacatag ataaaacatt tggtctgatt ttctcacaac
     1141 gtgtcttatt agcattaatt aataaaggta tggtccgtga agaagcatac gacaaagtac
     1201 aaccaaaagc gatgatatct tgggaaacga aaacaccttt ccgtgaatta atcgaacaag
     1261 atgagtctat tacaagtgtt ttaacaaaag aagaattgga tgaatgtttc gatcctaaac
     1321 atcatttgaa tcaagttgac actattttcg aacgcgctgg attagcataa aattaaataa
     1381 agtaacgatt cagatttaat aaaatataaa tcgctacact ttaataggtt aatgcgttaa
     1441 aatgacataa agaataaaat tataggggtt gtttcgatat gcaaattgaa aaattacgag
     1501 gtgcagcgtt agatgaattg tttgatgcaa tactaacgtt agaaaataga gaagaatgtt
     1561 accaattttt cgatgatttg tgtactgtaa atgaaattca atcactgtct caacgattac
     1621 aagttgctaa aatgattaag caaggttata cctatgcaac gattgaacaa gaatctggag
     1681 catcgactgc aacgatttct agagtgaagc gttcattaca atggggtaat gatgcttata
     1741 caatgatttt agatcgtatg aatattgaaa caaatgaata ataaatgaac gataaacaat
     1801 ggttatctat ctgtactaat aaggtagata atcattgttt tttcacgaaa aaatttacag
     1861 agtaaaagaa cttaaatttc atattaagtc tttagaactc gacacttaaa aatgctataa
     1921 tcatatgtat gttaaaaaag gagtttcgga aaatgtatga cattaaaaaa tggcgccata
     1981 tttttaaatt agacccagct aagcatattt cagatgatga tttagatgcg atttgtatgt
     2041 ctcaaacaga tgcaattatg attggtggaa ctgatgacgt tactgaagat aatgtcattc
     2101 atttaatgag cagggtaaga agatacccat tgcctttagt acttgaaata tcaaacatcg
     2161 aaagtgtaat gcctggtttt gatttttatt ttgtacctac agtactgaac agtacagatg
     2221 ttgcatttca caatggtaca ttattagaag cgcttaaaac atatggacat agtatagatt
     2281 ttgaagaagt aatatttgaa gggtatgtcg tgtgcaatgc tgatagcaaa gtggcaaaac
     2341 ataccaaagc aaatacagat ttaacaacag aagatttaga agcatatgcc caaatggtca
     2401 atcatctgta tcgattaccg gttatgtata tagagtatag tggcatttat ggcgacgtat
     2461 caaaggttca agctgtctca gaacatctaa cagaaacgca acttttttat ggtggcggta
     2521 tttcctcaga acaacaagcg acagagatgg cagctattgc agatacaatt atcgtcggtg
     2581 atattattta taaagatatt aaaaaagctt taaaaacagt aaaaataaag gagtctagta
     2641 aatgaatgcg ttattaaatc atatgaatac agagcaaagt gaagctgtaa agacaacaga
     2701 aggaccattg ttaattatgg caggtgctgg ttcagggaag acacgtgttt taacacatag
     2761 aattgcttat ttattagacg aaaaagatgt ctcaccatac aatgttttgg ctattacttt
     2821 tacaaataaa gcagcaagag aaatgaaaga acgtgttcaa aaattagtag gtgatcaagc
     2881 agaagtgatt tggatgtcaa cattccactc aatgtgtgtg cgtattttac gtcgtgatgc
     2941 agatcgaatt ggtatagaac gcaattttac gataattgat cctacagacc aaaaatctgt
     3001 tattaaagac gtcttaaaaa atgaaaatat tgatagtaaa aagtttgaac cacgtatgtt
     3061 tatcggtgcg atcagtaatt tgaaaaatga acttaaaaca cctgcagatg ctcaaaaaga
     3121 agccacagat tatcactcgc aaatggtagc aacggtttat agtggatatc aacgccaatt
     3181 gtcacgtaat gaagcgttag attttgatga ccttattatg acaacgatta acttatttga
     3241 gcgtgtacca gaagttctag aatattatca gaacaaattc caatatattc atgtagatga
     3301 gtatcaagat accaataaag cacaatacac attagttaaa ttattagcaa gtaagtttaa
     3361 aaacttatgt gttgtaggtg actcagatca gtcaatttat ggttggcgtg gtgctgatat
     3421 tcaaaatatc ttatcatttg aaaaagacta cccagaagcg aatacaatct ttttagagca
     3481 aaattatcgt tcgacgaaaa cgattttaaa tgcggctaac gaagtgatta aaaataattc
     3541 tgaacgtaag ccaaaaggac tgtggactgc aaatacgaat ggtgagaaaa ttcattacta
     3601 tgaagcaatg acagaacgtg atgaagcgga atttgtaata cgagaaatta tgaagcatca
     3661 acgtaatggt aagaaatatc aagatatggc aattttatat agaacaaatg cacaatcacg
     3721 tgtacttgag gaaacattca tgaaatctaa tatgccatac acaatggttg gtggccaaaa
     3781 gttctatgac cgtaaagaaa ttaaagattt attaagttat ttgcgtataa ttgccaatag
     3841 taatgatgac attagtttgc aacgtattat taatgttcca aaaagaggtg taggtccttc
     3901 atctgttgaa aaagttcaaa attatgcact tcaaaacaat atcagtatgt ttgatgcact
     3961 tggagaagct gattttatcg gattgtcaaa aaaggtgaca caagagtgtc ttaattttta
     4021 cgagttaatc caaagcttga taaaagaaca agaattttta gaaattcatg aaatagttga
     4081 tgaagtatta caaaagtctg gctaccgaga aatgcttgaa cgtgaaaata cactagaatc
     4141 tcgaagtaga ttagaaaaca tcgatgaatt tatgtcagta ccaaaagact atgaggaaaa
     4201 taccccatta gaagaacagt cattaattaa ctttttaacg gatttatcgt tagtagctga
     4261 tattgatgag gcagatactg aaaatggcgt aacactaatg acgatgcact cggctaaggg
     4321 ccttgaattt ccaatcgtct ttataatggg aatggaagaa tctttattcc cacatattag
     4381 agcgattaag agtgaagatg atcatgaaat gcaagaagaa cgtcgtattt gttatgtagc
     4441 aatcacaagg gctgaagagg tgttatacat cactcatgcg acatcaagaa tgttatttgg
     4501 tcgccctcag tcaaatatgc catctagatt tttaaaggaa attccagaat cactattaga
     4561 aaatcattca agtggcaaac gacaaacgat acaacctaag gcaaaacctt ttgctaaacg
     4621 tggatttagt caacgaacaa cgtcaacgaa aaaacaagta tcgtcatctg attggaatgt
     4681 aggtgacaaa gtgatgcata aagcctgggg agaaggcatg gtgagtaatg taaacgagaa
     4741 aaatggctca atcgaactag atattatctt taaatcacaa gggccaaaac gtttgttagc
     4801 gcaatttgca ccaattgaaa aaaaggagga ttaagggatg gctgatttat cgtctcgtgt
     4861 gaacgagtta catgatttat taaatcaata cagttatgaa tactatgtag aggataatcc
     4921 atctgtacca gatagtgaat atgacaaatt acttcatgaa ctgattaaaa tagaagagga
     4981 gcatcctgag tttaagactg tagattctcc aacggttaga gttggtggtg aagcccaagc
     5041 ctctttcaat aaagtcaacc atgacacgcc aatgttaagt ttagggaatg catttaatga
     5101 ggatgatttg agaaaattcg accaacgcat acgtgaacaa attggcaacg ttgaatatat
     5161 gtgcgaatta aaaattgatg gcttagcagt atcattgaaa tatgttgatg gatacttcgt
     5221 tcaaggttta acacgtggtg atggaacaac aggtgaagat attaccgaaa atttaaaaac
     5281 aattcatgcg atacctttga aaatgaaaga accattaaat gtagaagttc gtggtgaagc
     5341 atatatgccg agacgttcat ttttacgatt aaatgaagaa aaagaaaaaa atgatgagca
     5401 gttatttgca aatccaagaa acgcagctgc ggggtcatta agacagttag attctaaatt
     5461 aacggcaaaa cgaaagctaa gcgtatttat atatagtgtc aatgatttca ccgatttcaa
     5521 tgcgcgttcg caaagtgaag cattagatga gttagataaa ttaggtttta caacgaataa
     5581 aaatagagcg cgcgtcagcg atatcgatgg cgttttagaa tatattgaaa aatggacaag
     5641 ccaaagggaa tcattgccat acgatatcga tggtattgtg ataaaggtta atgatttaga
     5701 tcagcaggac gaaatgggat tcacacaaaa atcaccaaga tgggccattg cttataaatt
     5761 cccagctgag gaagtggtaa ctaaattatt agatattgaa ttaagtattg gacgtacagg
     5821 tgtagtcaca cctactgcta ttttagaacc agtaaaagta gctggtacaa ctgtatcaag
     5881 agcatccttg cacaatgaag atttaattca tgacagagat attcgaattg gtgatagtgt
     5941 tgtagtgaaa aaagcaggtg acatcatacc tgaagttgta cgtagtattc cagaacgtag
     6001 acctgaggat gctgtcacat atcatatgcc aacccattgt ccaagttgtg gacatgaatt
     6061 agtacgtatt gaaggcgaag tagcacttcg ttgcattaat ccaaaatgcc aagcacaact
     6121 tgttgaagga ttgattcact ttgtatcaag acaagccatg aatattgatg gtttaggcac
     6181 taaaattatt caacagcttt atcaaagcga attaattaaa gatgttgctg atattttcta
     6241 tttaacagaa gaagatttat tacctttaga cagaatgggg cagaaaaaag ttgataattt
     6301 attagctgcc attcaacaag caaaggacaa ctctttagaa aatttattat tcggtctagg
     6361 tattaggcat ttaggtgtta aagcgagcca agtgttagca gaaaaatatg aaacgataga
     6421 tcgattacta acagtaactg aagcggaatt agtagaaatt catgatatag gtgataaagt
     6481 agcacaatct gtagttactt atttagaaaa tgaagatatt cgtgctttaa ttcaaaaatt
     6541 aaaagataaa catgttaata tgatttataa aggtatcaaa acatcagata ttgaaggaca
     6601 tcctgaattt agtggtaaaa cgatagtact gactggtaag ctacatcaaa tgacacgcaa
     6661 tgaagcatct aaatggcttg catcacaagg tgctaaagtt acaagtagcg ttactaaaaa
     6721 tacagatgtc gttattgctg gtgaagatgc aggttcaaaa ttaacaaaag cacaaagttt
     6781 aggtattgaa atttggacag agcaacaatt tgtagataag caaaatgaat taaatagtta
     6841 gaggggtatg tcgatgaagc gtacattagt attattgatt acagctatct ttatactcgc
     6901 tgcttgtggt aaccataagg atgaccaggc tggaaaagat aatcaaaaac ataacaatag
     6961 ttcaaatcaa gtaaaagaaa ttgctacgga taaaaatgta caaggtgata actatcgtac
     7021 attgttacca tttaaagaaa gccaggcaag aggactttta caagataaca tggcaaatag
     7081 ttataatggc ggcgactttg aagatggttt attgaactta agtaaagaag tgtttccaac
     7141 agacaaatat ttgtatcaag atggtcaatt tttggacaag aaaacaatta atgcctattt
     7201 aaatcctaag tatacaaaac gtgaaatcga taaaatgtct gaaaaagata aaaaagacaa
     7261 gaaagcgaat gaaaatttag gacttaatcc atcacacgaa ggtgaaacag atcctgaaaa
     7321 gattgcagaa aaatcaccag cctatttatc taacatttta gagcaagatt tttatggtgg
     7381 tggagataca aaaggtaaga atattaaagg tatgacgatt ggtttagcta tgaatagtgt
     7441 ttattactat aaaaaagaaa aagatggacc gacttttagt aaaaaactag atgatagcga
     7501 agttaaaaag caaggtaaac aaatggctag tgagatatta tcaaggttac gtgaaaatga
     7561 tgatttaaaa gatataccaa ttcattttgc aatttataag caatcaagtg aagattcaat
     7621 cacaccaggt gaatttatca ctcaagcgac tgcagaaaag agtcaaacaa agcttaatga
     7681 atggcataat atcaatgaaa aatcagcttt attaccttct tcaacagcag cagattatga
     7741 tgaaaattta aataataatt tcaagcaatt taatgataat ttgcaatcat atttttctaa
     7801 tttcacacaa gcagtaggaa aagttaaatt tgttgataaa aagccacaac gattagtagt
     7861 agatttacca atcgattact atggacaagc tgaaacaatt ggtattacac agtacgttac
     7921 tgaacaagcg aataaatatt tcgataaaat cgataactat gaaattcgga ttaaagatgg
     7981 taaccaacca cgtgctttaa ttagtaagac aaaagatgac aaagaaccgc aagttcatat
     8041 ttacagtaat taattgtaaa ttaaagataa taaaaaggta ccaatgttag tcataatttt
     8101 gattaacatt agtacctttt taatttgtta agacttatga attatttttc tctaacgatg
     8161 tcacgaactt tgtttaagtc agtttcaaca aatgcaccag gttttttagt aagtttactt
     8221 acaacatatg taacgattac acttacaata aatccaggaa taatttcata taagccgaat
     8281 atttcgttga tatgtgccaa tggtttaatc cacgcaatcc aaacgataac gactaaggca
     8341 cctgaaacca ttccacttac agcaccggca cgtgtcaaac ctttccagta aagtgcaaat
     8401 agcacaagtg gactgaacga tgcaccaaat ccggcccaag cattacctac taagtttaga
     8461 attgtgtcgt ttggattcca tgcaatcgcg atggcaacaa ttgctactgc taatacagat
     8521 aaacgtccaa ccataacgaa ttctttttga tgtgttttag ctttttcttc accacgaact
     8581 aatttataaa agtcttcagt taaagaacta gacgttacaa gtaattgtga agaaatggtg
     8641 ctcataattg ctgctagaat cgcagcaagt aagaaaccac ctacaagagg atggaagagt
     8701 acttgactca tcacgatgaa taatgtctca ggatcttcta gtttaatatg ataagcaggt
     8761 acgaatgcaa tacctgttaa accaacagcc acagcgccta ataaaccaac agccatccag
     8821 ctaataccta aacgtctagc tttaggtagc atcttgtgtg atttaataga cataaacctt
     8881 acaatgatat gaggttgacc gaaataacct aatccccatg aaaatagaga gataattcct
     8941 ataaatgata accctttaaa taaatttaaa tttgtaggtt tcatagctgc tacatcatga
     9001 aacgttcccc agccgtttaa attcatcata gcaacaatag ggaccataac catcgcaatt
     9061 aacataatga caccttggaa gaaatctgta attgatacag ctaaatatcc accaaagaaa
     9121 gtatagaaaa tgacaatgaa agcaactaat attaaaccga aatgataatc taatccaaaa
     9181 gcactttcaa ataatttacc accagatacg aaaccagaat gtgtatataa tgtaaagaat
     9241 actacgataa tcaatccaga aataatcttt aacacattat ttttatcgtt taaacgattt
     9301 ttaaagaaat ctggtaatgt aattgcatct ccagctaatt cggtataaac gcgaagtcta
     9361 ggagcaacaa caaagtaatt tatataagca cctaatgtta agccgattgt aatccacatt
     9421 gccgaaagac cagtactata gacagaacca ggtagaccca taatcatcca tccactcata
     9481 tctgaagctc cagctgataa tgcagtaata tacggtccaa tactacgtcc gcctaacatg
     9541 tactcgctta ggttaccagt tgcttgcttg taaccgtaaa agccaataac gattagtatc
     9601 aagaagtaga cggcaatcat aatataagtt tgccaattgg catctacttg ttgacttaat
     9661 gctgtcccca ttgtaagcat gataaacact ccctttattt aatttataat ttagttactt
     9721 gaaaacatta tacataaaga taaatgttta gaaaagtagg aaaattaaat ttattctttt
     9781 tgaaatactt gtataccaat atagaaaacg ctttcgtatt ttttagatat gagcaaattt
     9841 ttataattaa aattgcataa tcataatatt gaattgggtt gtctaataat aaaagggtgt
     9901 tgttttcata aaaaatgatt tggtaaacga atgataatgt ttatacagtt tatatgaaat
     9961 taaagtagta cctcataaat acttagattt ttaattggaa atttgataca atttagtgat
    10021 gaatgactta aaggaggctt ttattaatga caaaagtaac acgtgaagaa gttgagcata
    10081 tcgcgaatct tgcaagactt caaatttctc ctgaagaaac ggaagaaatg gccaacacat
    10141 tagaaagcat tttagatttt gcaaaacaaa atgatagcgc tgatacagaa ggcgttgaac
    10201 ctacatatca cgttttagat ttacaaaacg ttttacgtga agataaagca attaaaggta
    10261 ttccgcaaga attagctttg aaaaatgcca aagaaacaga agatggacaa tttaaagtgc
    10321 ctacaatcat gaatgaggag gacgcgtaag atgagcattc gctacgaatc ggttgagaat
    10381 ttattaactt taattaaaga caaaaaaatc aaaccatctg atgttgttaa agatatatat
    10441 gatgcaattg aagagactga tccaacaatt aagtcttttc tagcgctgga taaagaaaat
    10501 gcaattaaaa aagcgcaaga attggatgaa ttacaagcaa aagaccaaat ggatggcaaa
    10561 ttatttggta ttccaatggg tataaaagat aacattatta caaacggatt agaaacaaca
    10621 tgtgcaagta aaatgttaga aggttttgtg ccaatttacg aatctactgt aatggaaaaa
    10681 ctacataatg agaatgccgt tttaatcggt aaattaaata tggatgagtt tgcaatgggt
    10741 ggttcaacag aaacatctta tttcaaaaaa acagttaacc catttgacca taaagcagta
    10801 ccaggtggtt catcaggtgg atctgcagca gcagttgcag ctggcttagt accatttagc
    10861 ttaggttcag acactggtgg ttcaattaga caaccggctg catattgtgg tgttgtcggt
    10921 atgaaaccaa catacggtcg tgtatctcga tttggattag ttgcatttgc atcttcatta
    10981 gaccaaattg gtccattgac tcgaaatgta aaagataatg caatcgtatt agaagctatt
    11041 tctggtgcag atgctaatga ctctacaagt gcacctgttg atgatgtaga ctttacatct
    11101 gaaattggta aagatattaa aggattaaaa gttgcattac ctaaagaata cttaggtgaa
    11161 ggtgtagctg atgacgtaaa agaagcagtt caaaacgctg tagaaacttt aaaatcttta
    11221 ggtgctgtcg ttgaggaagt atcattgcca aatactaaat ttggtattcc atcatattac
    11281 gtgattgcat catcagaagc ttcgtcaaac ctttctcgtt ttgacggaat tcgttatggt
    11341 tatcattcta aagaagctca ttcattagaa gaattatata aaatgtcaag atctgaaggt
    11401 ttcggtaaag aagtaaaacg tcgtattttc ttaggtacat ttgcattaag ttcaggttac
    11461 tacgatgctt actataaaaa atctcaaaaa gttagaacat tgattaaaaa tgactttgat
    11521 aaagtattcg aaaattatga tgtagtagtt ggtccaacag cgcctacaac tgcgtttaat
    11581 ttaggtgaag aaattgatga tccattaaca atgtatgcca atgatttatt aacaacacca
    11641 gtaaacttag ctggattacc tggtatttct gttccttgtg gacaatcaaa tggccgacca
    11701 atcggtttac agttcattgg taaaccattc gatgaaaaaa cgttatatcg tgtcgcttat
    11761 caatatgaaa cacaatacaa tttacatgac gtttatgaaa aattataagg agtggaaatc
    11821 atgcattttg aaacagttat aggacttgaa gttcacgtag agttaaaaac ggactcaaaa
    11881 atgttttctc catcaccagc gcattttgga gcagaaccta actcaaatac aaatgttatc
    11941 gacttagcat atccaggtgt cttaccagtt gttaataagc gtgcagtaga ctgggcaatg
    12001 cgtgctgcaa tggcactaaa tatggaaatc gcaacagaat ctaagtttga ccgtaagaac
    12061 tatttctatc cagataatcc aaaggcatat caaatttctc aatttgatca accaattggt
    12121 gaaaatgggt atatcgatat cgaagtcgac ggtgaaacaa aacgaatcgg tattactcgt
    12181 cttcacatgg aagaagatgc tggtaagtcg acgcataaag gtgagtattc attagttgac
    12241 ttgaaccgtc aaggtacacc gctaattgaa atcgtatctg aaccagatat tcgttcacct
    12301 aaagaagcat atgcatattt agaaaaatta cgttcaatta ttcaatacac tggtgtatca
    12361 gacgttaaaa tggaagaggg atctttacgc tgtgatgcta acatctcttt gcgtccatat
    12421 ggtcaagaaa aatttggtac taaagctgaa ttgaaaaact taaactcatt taactatgta
    12481 cgtaaaggtt tagaatatga agaaaaacgc caagaagaag aattgttaag tggtggagaa
    12541 atcggacaag aaacacgtcg atttgatgaa tctacaggta aaacaatttt aatgcgtgtt
    12601 aaagaaggtt ctgaggatta ccgttacttc ccagagcctg acattgtacc tttatatatt
    12661 gatgatgctt ggaaagagcg tgttcgtcag acaattcctg aattaccaga tgagcgtaaa
    12721 gctaagtatg taaatgaatt aggcttacct gcatacgatg cacacgtatt aacattgact
    12781 aaagaaatgt cagatttctt tgaatcaaca attgaacacg gcgcagatgt taaattaaca
    12841 tctaactggt taatgggtgg cgtaaacgaa tatttaaata aaaatcaagt agaattatta
    12901 gatacaaaat taacaccaga aaatttagca ggtatgatta aacttatcga agacggaaca
    12961 atgagcagta aaattgcgaa gaaagtcttc ccagagttag cagctaaagg tggtaatgct
    13021 aaacaaatta tggaagataa tggcttagtt caaatttctg atgaagcaac acttctaaaa
    13081 tttgtaaatg aagcattaga caataacgaa caatcagttg aagattacaa aaatggtaaa
    13141 ggcaaagcta tgggcttctt agttggtcaa attatgaaag cgtctaaagg tcaagctaat
    13201 ccacaattag taaatcaact attaaaacaa gaattagata aaagataatt agattcatca
    13261 gactatgatg atataacaat gaacccttgg ttgctgactt agatgcaatc gagggtttat
    13321 ttatatctat agaagtcata ttacttttaa ctttattcat tgtacatgtt aatggtaaaa
    13381 atattaattt tattaatgcg ttagctttaa ttatattaag gcaaactgta taataaaaag
    13441 gtataaaaca tttttgtata aagacaacat tatatttaca acatcatttt aaaggtaaaa
    13501 tagcataact gacgaagtct atataacgaa gaacggcaaa aaatgctgaa taaataacaa
    13561 cctttgtaca tattgagata gtatttgttt aagatacaag ttggtcttta acgatattaa
    13621 gaatgatgag ataagactga tcctgggcca taaattcaat gtcctaggca ctacaatgtt
    13681 atattggcag tagttgactg aaagaaaata cgcttgtaat aagctttttt caattctagt
    13741 ggggccccaa caaagaagct gactttctgt cagcttacaa taatgtgcaa gttagggtgg
    13801 ggccccaaca aagagaattt cgaaaggaaa ttctacagac aatgcaagtt ggcggggccc
    13861 caacatagaa gctgactttc tgtcagctta caataatgtg caagttgggg tggggcccca
    13921 aacatagaga attttgaaaa gaaattctac agacaatgta agttggggta gaacgaaata
    13981 aattttgtta aatattattt ctgtcccact cctattagac gaaaccaaga tgaagttaaa
    14041 atatatgaat tttaagtaga gggataagat atgaggaaac gtgctagaat catttataac
    14101 ccgacatcag gtaaagagca atttaaaaga gaattacctg atgccttaat aaaattagaa
    14161 aaagcgggat atgaaacgag tgcatatgca accgagaaaa taggtgatgc cacacttgaa
    14221 gcagaaagag ctatgcatga aaattatgat gtattaatcg ctgcaggtgg tgacggaaca
    14281 ttaaatgaag tagttaatgg tatcgcagaa aagcctaatc gtcctaagct aggtgtcatt
    14341 cctatgggta ctgttaatga ctttggacgt gcattgcata tacctaatga catcatgggg
    14401 gcacttgatg tcatcattga aggtcattct actaaagtag atattggtaa aatgaataat
    14461 cgatacttta ttaatttagc tgcaggcgga caattgacgc aagtctctta tgaaacaccg
    14521 agtaaattga aatctattgt tggtccattt gcttattaca tcaaaggttt cgaaatgtta
    14581 cctcaaatga aagctgtaga tttaagaatt gaatatgatg gtaatgtttt ccaaggagaa
    14641 gcattattat tctttttagg tttaacaaat tcaatggcag gatttgaaaa attagtgccg
    14701 gacgctaagt tagatgacgg ctattttacg ttaattatag ttgaaaaatc aaaccttgca
    14761 gaacttggtc atattatgac tttagcttca agaggagaac atactaagca tcctaaagtt
    14821 atttatgaaa aagcaaaggc aattaatatt tcatcattca cagacttaca actaaatgta
    14881 gatggagaat atggtggtaa attaccagcg aatttcttga atctagaacg tcatatagat
    14941 gtctttgcac caaatgatat tgtaaatgaa gagttaataa ataatgatca tgttgatgac
    15001 aatttaatcg aagaataaac agaagtagtg aaatatataa tggcaggtta ggaagcgcat
    15061 aattttgttc ctaacctgtt cattttggag gtaaaatttt gcaagcaata gctaaaaatg
    15121 acattaaaac gggaacagtt gttgatttaa cgcatgaagg tcatggtgtc gtaaaaatag
    15181 atcgctttcc tattttcata ccacaagctt taattaatga acaaattgaa tataaaatta
    15241 tcaaagtaaa aaagaacttt gcgataggta agttgcttaa tataaatacg agaagtgaga
    15301 atcgtgttgc accgccatgt atatattatg aacgttgtgg tgggtgccaa cttcaacact
    15361 tatcatacga agcacaactt gaaatgaaaa aggaacaagt gattaatttg tttcagcgaa
    15421 aagcgcattt tgataattct aagattaacg atacagttgg catgacagat ccttggagat
    15481 atcgaaacaa gtcccagatt ccagttggta agaatgaaca gaatgaagtt attatgggct
    15541 tttatcgtca aagaagccat gacattattg acatgggaag ttgtttaata caagattcac
    15601 aacatcaaga agtgatgaat gaagttaaat ctattttaaa agatttaaat gtaagtattt
    15661 atcaagaaca attgaaaaaa ggtttaatga gacatttggt tgtaagaaca ggatatcata
    15721 cagacgaaat gatggttatt tttgtaacta atggtaaaaa ttggccacaa aaaaatgctg
    15781 ttgttgagaa gatattagat gcatttccta atgtgacaag cattaaacaa aacattaatg
    15841 atagccactc taatgttatt atgggacgtc agtctataac gctatatggt aaagatacaa
    15901 ttattgatca attaacagat agtacattta aaataagtga tcagtcattc taccaaatta
    15961 attctgaaca aacagagaaa ttatataata aagcaattga gtatgcgcaa ttgacaggta
    16021 atgaagttgt attagatacc tattgtggta ttggaacaat aggactatat atggcaccac
    16081 atgcaaaaca tgtatacggt gttgaagttg taccttctgc aattgaagac gcacaacaaa
    16141 atgcgactat aaaccaatgc aataatacaa cttttgtctg tgggaaagct gaagaagtta
    16201 ttttacaatg gaaagcacaa gggattaaac cagatgttgt aatggttgat ccgccaagaa
    16261 agggctgtga cgaaacgttt attcagactt tgcttacatt agaacctaag cgtattgtct
    16321 atatttcatg taatccctca acacagcaac gtgatgcatt attattagct gaaaaatatc
    16381 aacttgaaga ggtaacaccg gtagatatgt ttccacaaac aacacatgtt gagacagtgg
    16441 cattattcaa tttgaaatag cgcacatgat ttgagataaa atttcgcctt aataagtgaa
    16501 gaaagaagtc gtatattaaa attttattac agccaattta ctttcttgat atgaactttt
    16561 taaattaaat gtgacattgt atactatatt taaagaagaa taagaatgtc atgatcggga
    16621 gggttggtaa tgcataaaat tgatttaacg acaaataatt ttcaaatgcg aaggtttatt
    16681 atcttgcaac ttgttattgc gttattcgtg attttattta cgtataagtg ggcgttaggt
    16741 gtcacagcgg ttgtcgatca aaatatcatc atcaatcttg tatatgggtt tgctggattc
    16801 attattttat tgattttaca tgaacttatc catagagcat tatttctgtt atttaaaaaa
    16861 gatagcaaac ctatgtttaa tataaaaaaa gacagaatct tattccaaac agcagatgct
    16921 tgcttcaaca aatggcaatt ctcaattatt atgctgtcac cactaatcct attaagtgct
    16981 ggcttgctta ttttaattaa ggtatttggt tattcatcac ttatttttat gtttagtatg
    17041 cataccgctt attgctttat agatattctg cttgttgcat taacaatatc tagtagtttt
    17101 aaatatgttc aacaagatga agatagtatt tatttgtatc atcaaaagcc gactcaataa
    17161 taaattttaa tatgatgata ccagcaaaat gaatttgctt gaattatttc agattaccta
    17221 tgttttctta ctcaatcgtc ttcatcatca tgcttaaata gcaattgatt ttataacaac
    17281 ttgtagttat ctcaatatag ttatcgctaa gtgcaagatt tatgtaaaaa aatcgtacac
    17341 agcgtataat tatgttgaga tatttttgtg cgttataaaa gtaaaaatta gtagggaggt
    17401 gagcacttga ctgagagacg aattattcat atcgatatgg actatttttt tgcacaagtc
    17461 gaaatgagag ataaccccaa attgaaaggg aaaccagtca ttgttggtgg taaagcaagt
    17521 agtaggggcg ttgtctctac ggcatcttat gaagcacgaa aatacggcgt ccattcagca
    17581 atgcctatgt cacaagcgca taaattatgt ccaaatggat actttgtcac aagtaatttt
    17641 ggtgcttacc gagaaacatc tgcgcagatt atgtctattt tccgtagtta tacagataag
    17701 gtagaaccga tgtcattaga tgaagcgtat ttagatatta cagaattagt gagaccagac
    17761 cttcctgctt cgaaaattgc tcagtatatt agaaaagata ttctagagca gacacattta
    17821 acagcatctg caggtgtttc ttataacaaa tttttagcta aattagcgag tggtatgaat
    17881 aaacctgatg gtatgactgt gattgattat cgaaatgtcc atgatatttt gatgacattg
    17941 gatattggag attttccagg cgtaggtaaa gcttccaaaa aagtaatgca tgataatggt
    18001 atttttaacg gtagagattt atatgagaaa acggaatttg aattaatacg tttgtttgga
    18061 aaaagaggtc ggggtttata taacaaggca cgcggtattg accatagtga agtgaaatca
    18121 acaagagtaa gaaaatcagt agggactgaa cgcacatttg caacagacgt gaatgatgat
    18181 gaagagattt taagaaaagt atgggagttg tcaggtaaaa cagctgaacg tctaaataaa
    18241 ttacagaagt cggctaaaac tgtaacggtt aaaattaaaa cttatcaatt tgaaacgtta
    18301 tctaaacaga tgagtttaag agattctgtt agttctgaag aagatattta taatattgca
    18361 tatttacttt ataacgattt aaaagaccct gatgtaccaa ttcgacttat tggtgtcact
    18421 gtaggtaatt tagaacaatc aacttataaa aatatgacga tatatgactt tatataaaat
    18481 aaagctccct tcaaagttta catttttaca atgcttactt tgaagggagt attttattta
    18541 gtcctagcct ttatctttta gatttttacc ataaatatta gttacatatg ttaagttttc
    18601 ataatttttc agtagcctaa aagtaatcat tgcgcatgct ttggcatcat tcaatgcatc
    18661 atgatgacca tgaaaatcta attgataaaa ctccatcata tgttttaaac cgtatcgata
    18721 cgaatcaacg gttcttttag caagttgata actacaaaag taagttaaat ttggagttgg
    18781 taaaccaata ttttgaatgc tttgatgtaa gacgttcata tcaaatgccg cgttatgtgc
    18841 gacaacaggt aaatctgcaa taaattgcat catatatgga aatacgtagt caaacgtagg
    18901 tgcattttca acatcttctg gttgtatgcc atgaacttta atattttgtt gtgaaaaata
    18961 gtcttgcgga ttcacaagag tatgaaatgt ttctgttatt tgactatcaa tgactttaac
    19021 cattccgaca gaacaaatac ttgtacgttt accatttgct gtttcaaaat caagtgcaac
    19081 aaacgcatct tgtatcatgt agtcaatcct ttcaattaat taaatgtata tattaataaa
    19141 ctttt
//
LOCUS       DATTQF010000037        18772 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.037, whole genome shotgun sequence.
ACCESSION   DATTQF010000037 DATTQF010000000
VERSION     DATTQF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.037"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(190..573)
                     /locus_tag="VB241_001137"
     CDS             complement(190..573)
                     /locus_tag="VB241_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEP1244440.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKKNLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLGF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            756..2198
                     /locus_tag="VB241_001138"
     CDS             756..2198
                     /locus_tag="VB241_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HEP1244441.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPV
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            complement(2284..3228)
                     /locus_tag="VB241_001139"
     CDS             complement(2284..3228)
                     /locus_tag="VB241_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP1244442.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3274..3897)
                     /locus_tag="VB241_001140"
     CDS             complement(3274..3897)
                     /locus_tag="VB241_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1244443.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            4058..5335
                     /locus_tag="VB241_001141"
     CDS             4058..5335
                     /locus_tag="VB241_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP1244444.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGIQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLISAIVRKHSTKRTEVPS"
     gene            5541..5972
                     /locus_tag="VB241_001142"
     CDS             5541..5972
                     /locus_tag="VB241_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HEP1244445.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            6072..6188
                     /locus_tag="VB241_001143"
     CDS             6072..6188
                     /locus_tag="VB241_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244446.1"
                     /translation="MANYAIYFNNININSIMSLNDNNYQFERVNERNEYRES"
     gene            6199..7233
                     /locus_tag="VB241_001144"
     CDS             6199..7233
                     /locus_tag="VB241_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1244447.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKSERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHAVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            7269..7670
                     /locus_tag="VB241_001145"
     CDS             7269..7670
                     /locus_tag="VB241_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1244448.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSQIMEHIMKYIKNVAVESTYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            complement(8236..9240)
                     /locus_tag="VB241_001146"
     CDS             complement(8236..9240)
                     /locus_tag="VB241_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEP1244449.1"
                     /translation="MTETFKAFVIDKDESGKVTPTFKQLSTTDLPKGDVLIKVHYSGI
                     NYKDALATQNHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIVKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSNQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            9430..9945
                     /locus_tag="VB241_001147"
     CDS             9430..9945
                     /locus_tag="VB241_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1244450.1"
                     /translation="MAGIIKEISKQDASELVELATRTFYDTFGSYYDDNDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIALAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            10261..10890
                     /locus_tag="VB241_001148"
     CDS             10261..10890
                     /locus_tag="VB241_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244451.1"
                     /translation="MKKLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSNSGQTTN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKSQALQQLPNFQTALNAANNEANKFGNGHK
                     VYNDYSIEEHNGNYKYVFSFKDPNVNGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11044..12717)
                     /locus_tag="VB241_001149"
     CDS             complement(11044..12717)
                     /locus_tag="VB241_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEP1244452.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHAVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNWPDSWLYSTKFMLPKWLYKIAKQRADNKQLQNLG
                     LYTKQPNVLKDHIVFIGDHYQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVLVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDETMLPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQQKEQPLESISQLILSKMTSNK"
     gene            13034..14632
                     /locus_tag="VB241_001150"
     CDS             13034..14632
                     /locus_tag="VB241_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEP1244453.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            15013..16491
                     /gene="mqo"
                     /locus_tag="VB241_001151"
     CDS             15013..16491
                     /gene="mqo"
                     /locus_tag="VB241_001151"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEP1244454.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKNVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVEIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(16618..17700)
                     /locus_tag="VB241_001152"
     CDS             complement(16618..17700)
                     /locus_tag="VB241_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1244455.1"
                     /translation="MKILAIVRRVLKEMLRDKRTLALMFLAPILILTLMYFIFNGDEN
                     DLKIGIDTSVSSNITKQLPSSDIKTQTFHSHENIKSKIENNHLDGYIYQENQTLYVIY
                     TNEDPNKSNMIKQMIGKAIQQDKISSIKHLIEKAPPTMKEPNKQDIKISHSYLYGNSD
                     STYFDKMFPILMGFFVFLFVFLISGIALLRERTTGTLERVLSTSIRRSEIVLGYLIGY
                     GIFAIIQTLIIVLFSIYLLNINLAGSLWYVILINICLAITALSMGIFISTFANSEFQM
                     IQFIPLVAVPQVFFSGIFPLENMPDWLGNIGYIFPLRYAGDALTNVMIKGQGWSNIWF
                     DLLILMIFIIVFIILNIVGLKRYRQV"
     gene            complement(17697..18425)
                     /locus_tag="VB241_001153"
     CDS             complement(17697..18425)
                     /locus_tag="VB241_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244456.1"
                     /translation="MEATIAKLDKVVKTYNKHHVLNEISMSINKGELLGLIGPSGSGK
                     TTTIKCLLGMEKIDSGSAEIFAHKMPNRKALNRLGYMGQTDALYENLTAHENLEFYGR
                     LSGLKGNILKDNIEKHIVLVNLEQALNKKVAQFSGGMKRRLSLAITLLAEPELIILDE
                     PTVGIDPKLRQQIWQQFKQMTKDGKSVVITTHVMDEAERCDKVGLIVDGRIFAIDTPT
                     NLKQRFNVDTIEAVFIKAEEVCQS"
     gene            18559..>18772
                     /locus_tag="VB241_001154"
     CDS             18559..>18772
                     /locus_tag="VB241_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1244457.1"
                     /translation="MDQTAFTDSRVTKTINRIHDGMVNLLKTKAYNDITVKDICEKSQ
                     ISRTTFYTHYKTKDDFIYSYLNTLLKE"
BASE COUNT         6382 a         2718 c         3322 g         6350 t
ORIGIN      
        1 taagggtata aattgattta tgctgtaact aattaaatgt gtatggtatc ttctaacatc
       61 atgaaacgca ttttaatata gatatgtaca tttaaacctt gagagataga ttatagtaaa
      121 ctttagtaca aagtatgaat tgctgaactc caatgactac tatttgtccg ttacaactaa
      181 agtttcaatc tatctctttc tttttgtgtt taaaacacga ctttgtagta aatattgaac
      241 atcatattta ttagcaagtt ctgctaaaaa acctaataaa gtttgggtgt cgattttacc
      301 attaataaaa tcagctttaa gtagttttga ttgttgtctt tcttcttcat tcgcacattt
      361 tttaaaatat ccccacatat gatcaaaagc attacataca cttcctatac tcggttcaat
      421 taacaaagca tcatcaatca tttgttggac ttgatgaagt gataaattct tttttaaagc
      481 attccttatc atttggtaag actgttgact gtgtaacaat acatgatatt tttcttcacg
      541 ccaaagctgt tcgatatggc cacgttcttt cataaaatta cctccgataa ttaatgttaa
      601 tatacttgca tgtagtatag caataaaaat gaatgcgcta tcaaaagtga tgtaattgtt
      661 tatataaaat gataatagag tgttgcaaat atttttggaa ccggttacaa taaagtggaa
      721 taaatggaac cggttctaat taggaggcta aatatatgaa ttataagcaa tccgcagaag
      781 atattttgaa cgcgataggc ggagaagaga atttagatgc aatggcgcat tgtgcaacga
      841 gactacgatt agttttaaat gatgaaagtt tagtaaatga agaggcgcta aacaatatgg
      901 atgtagttaa agggacattt tctactgggg gacaatacca aattattatt gggtctggta
      961 cagtcaataa agtatttagt gaactggaaa aattaactgg aaaagaagca tcaaccactt
     1021 cggaagtcaa agcacaatct gctaaaaata tgaatccggt acagcgattt gtaaaaatgc
     1081 tttcagatat ctttgttccg attataccag ccatcgttgc tggtggttta ttaatggggt
     1141 taaataacat tttgactgcg aaagatttat tcttttcagg taaatcattg atagatgtat
     1201 atagtcaatt tgctggttta gctgaaatga taaatgtttt tgcgaatgca ccatttacat
     1261 tattaccaat tttaattgga tttagtgcag caaaacgctt tggtggcaat ccgtttttag
     1321 gtgctgcatt aggtatgata ttggttcatc catcgctaat gagcgcatac gatttcccaa
     1381 aagcagttga agcaggtaag gctattccat attgggatgt atttggtttg catattaatc
     1441 aagtaggtta tcaaggacaa gtcttaccta tgcttgtagc agcttatatc ttagcctcaa
     1501 ttgaaaaagg gttacgcaaa gttattccaa cggtgttaga taatttgtta acaccattgt
     1561 tatctatttt tataacagca tttctaacat tttcatttgt aggtccaatc actcgacaat
     1621 taggttactg gttatcagat ggtttaacat ggctttatga atttggtggt gcaattggtg
     1681 gattaatttt cggattattg tatgctccga ttgttattac aggtatgcat catagcttta
     1741 tagctgtaga aacgacatta attgcagatg ccactaaaac aggtggatca tttatattcc
     1801 caattgcgac aatgtctaat gttgcacaag gtggtgcagc aattgcagcg ttctttatta
     1861 ttaaacaaaa taaaaagtta aaaggtgtgg catctgccgc aggtatttca gcattacttg
     1921 gtattacaga accggctatg tttggtgtta acttaaaact aagatatcca tttattggcg
     1981 ctatcgttgg atcaggtatt ggttcagcat atattgcttt cttcaaggtt aaagcaatcg
     2041 cattaggaac tgctggattg cctggattta tttcaatcaa tccagtacat gcaggatggt
     2101 tacactactt tgttggtatg acaatatcat tcatcgttgc tataacagtt actttaattt
     2161 tatcgaaaag aaaagcaaat aaagaagttg tagaataaaa aaagaggtat gacagtaatc
     2221 aaagtatcag tcattgcaaa tgcaatagga tgaaacttta agctgtcata ccttttaatt
     2281 tagttataat ttctgttttc gccataagaa aatactaaca attacgacaa gaattaatga
     2341 aatacctacc acaataatcc aagatatata actatggtca tcaattggca acggcacatt
     2401 cataccgaag aaactaaata cgagcgttgg taacgttaat agtaccgtga aaagtgttaa
     2461 tgtcttcata atcgtattca tatcattaga taataaggaa gcgtatgatg ttgtgatact
     2521 ttctaagata cgttgatgta attctgttgt ttcgatggct tggttatttt caatgattaa
     2581 atcttctagt aattcttcat cttcttcaaa acgtttaatt gcaggtaaac ggaataattt
     2641 tttaataatt gtatcgttac cttttaaggc agctaaaaag tatactaaac ttttttcaac
     2701 ttccattaat ttgaaaagtt gtttgttagt aatgttattt tttagttctt tctcaatacg
     2761 aattctactt ttattaagta atcgtaagtt acgattataa tggtcggcaa tagtagttaa
     2821 aatttctaat gcaaatctgc tgtgaaattt taaattaata tcacgcttag gtaaattttc
     2881 taaaaattca ttttcagcgt cacaaactgt tacgataata ccgttgccaa taataatgcc
     2941 taatggaatc gttacaaaag atagaacgct acgattagtt gaattgacga taggtaaatc
     3001 aataatgatt aatgagtaac cagtatcttc gtcatattca atacgggaac tttcttctga
     3061 gtccaaaggg tcacgtataa agtcttcagg tatattatat tgttccataa gattttcaat
     3121 ttcttctcgg tctggttcta ctacattaat ccatgacgct gtatgatcta atggtgtttt
     3181 tataatatgt ttatctatat cttcagaatg tctgaatgaa gtaatcatga tatatagttc
     3241 ctttctaaag gatttaggaa aagcgagtat tacttaagtt agtgaatcaa ggtttgattt
     3301 atgcaaacgg ttttctgcca ttattcttaa tggaccatta aaaacaatat tatgcacatg
     3361 tttagccaat tcatcaactg aaatgggctg tttgtccatt acccagtatt taataattga
     3421 aattgttgct ccggaaacgt aactgtaaaa atacatctct ggaatgcctc cgatagaatg
     3481 attgatacta atataacgtt gcatattatt tttaatatgt tcgttgattt tcaattctaa
     3541 ttgactcgtg cgttccaatt gtaaaatagt atgataaaac tctaaattat catgaatatg
     3601 ctggaaaata tttttgagta tattatttac aaaagtattc gcaatgtctt ctattgaaga
     3661 accgcgtaac aaatcaaatt gagtatatgt cgttagttct gaaatatact catcttccat
     3721 atctgcgagt aacatatatt tatcctcgta atgtaagtaa aatgtccctc tattgatatc
     3781 agcgcgtgtt gttatgtcgc gaatggtaat cttttctaaa tctttttctt gtagtaattt
     3841 agtgaacgct tgtttaatgg aggactttgt ctttctaatt cgcctatctt ctttcaaaaa
     3901 taaatcaacc tccttgtgtt attgaacaat gtgggtgtat ttgtctatta ataaacgtat
     3961 tgatgaacat tgtgtattgt ttattcccac ttacatatta taatagacaa catgcgaaat
     4021 attcaacaca ttgtctaata aggaggtaat taataaaatg aatatattta aaaataaatt
     4081 attatggatt gcaccaatag caactatgat tatcttggta attttttctt tagcttttta
     4141 tcctgcatat aatcctaaac caaaagattt accaattggt atattaaacg aggacaaagg
     4201 tacaacgatt caagataaaa atgttaacat tggtaaaaaa ttagaggata aattattaga
     4261 tagtgattct aataaaatta aatgggttaa ggttgatagt gaaaaagacc ttgaaaaaga
     4321 tttgaaagat caaaaaatct ttggagtagc tattattgat aaagactttt caaaagatgc
     4381 tatgagtaaa acacaaaaag tagttatgga tagcaaaaaa gaagaaatgc aacaaaaagt
     4441 tgcttcaggt gaaattccgc cacaagtagt tcaacaaatg aaacaaaaaa tggggaatca
     4501 acaagtagag gttaagcagg ctaaatttaa aacgattgta agtgaaggat caagcttaca
     4561 aggttcacaa attgcatcag ctgtattaac tggtatgggt gataatatta atgctcaaat
     4621 tacgaagcaa agtttggaaa cattaacgag tcaaaatgtt aaagtcaatg ccgcggacat
     4681 caatggtttg acgaatccag taaaagtgga taatgaaaaa cttaataaag ttaaagatca
     4741 ccaagcaggt ggtaatgcac cattcctaat gtttatgcca atttggatag gttcaatcgt
     4801 aacgtctatc ttattgttct ttgcatttag aactagtaac aatatcgtcg tgcaacatcg
     4861 tatcattgct tcaattggac aaatgatatt tgcagttgtt gcagcatttg caggtagctt
     4921 tgtttatatt tatttcatgc aaggcattca aggatttgat tttgaccatc caaatcgtat
     4981 cgcaattttt gtagcacttg cgattcttgg tttcgtgggc cttattttag gtgttatggt
     5041 atggctaggt atgaagtcaa ttccaatttt cttcatttta atgttcttta gtatgcaact
     5101 tgtaacgtta cctaaacaaa tgttgcctga aagttatcaa aaatatgtat atgattggaa
     5161 tccattcaca cactatgcaa caagtgtaag agaactatta tacttgaatc atcatattga
     5221 attaaatagt acaatgtgga tgtttatagg gtttatgatt tttggcgctg tatcaagttt
     5281 aatttcagct attgttagaa aacatagcac aaaacgtact gaagtgccgt cataatttaa
     5341 taaataggaa tatagctata caagacgttt gtagcgagcg tcatggtata gctattttat
     5401 ttgtatagca cttgatgtaa agttatttta gctgtcatac tatgtgctaa atcaaattca
     5461 gttgtgtcat atcttcgctt ttttgtcact gttataatag gtataatcgg atataatgaa
     5521 aaggtgaggg aggattcaaa atgcgtaggt tattatattc atttcttttt tatatgatca
     5581 taggtttatt tagtggcttt ttctatagag agctaacaaa agcatatgat tttactggta
     5641 caacacaatt atcacttgta cacacacata cacttatttt aggtatgttt atgtttttaa
     5701 tattattacc attggagaag ttatttaaat tatcaagtta ctacttattt aactggttct
     5761 tctatgtata taacataggt gttatcgtta ctataggtat gatggtgaca aaaggattct
     5821 tccaagtcac tggtaaatca tactctccag aagcatttgc aggctttgca ggtattgggc
     5881 acacaggtat gcttgcaggt ttattgttgc tatttttcct tttaagacaa gcaattctta
     5941 aagaaccacg tgatgagtct tttaaaaaat aatatgaact aaaaaatata cacatctttc
     6001 aaaggaacat tgttgtttcg tactttgaaa ggtgtgtttt tatattgtga tgggatagtt
     6061 attgataatt catggctaac tatgcgattt attttaacaa tataaatatt aatagtataa
     6121 tgtctttaaa tgataataat tatcaatttg agcgcgttaa tgaaagaaat gaatatagag
     6181 agtcatagga ggcacataat gaaagacgtt acaatcattg gtggcggtcc gtctggtttg
     6241 tatgcaagct tttatgctgg attgcgagat atgtctgtaa gattgataga tgttcaatct
     6301 gaattagggg gtaagatgag aatttaccct gaaaaaatta tttgggatat tggcggcatt
     6361 gcgccaaaac cttgtcatga aattttaaaa gatacaatta agcaaggatt atattttaaa
     6421 ccggaagttc atttgaatga acgtgtggta gatattagga aaaaatcaga acgtcatttc
     6481 gaagttgaaa cagaagcggg agaaatatat acatcgaaag cagttatcat tgctattggt
     6541 gcaggtatta ttaatccaaa acaattagat gttaaaggtg tagagagata tcaattaact
     6601 aatttacatt atgttgtaca aagttacaga cgtttcaaag ataaagatgt attaatatca
     6661 ggtggaggaa atacagcatt agattgggcg catgatattg ctaagattgc taaaagcgtg
     6721 acagttgttt atcgaaaaga agatgtaagt ggtcatgaag cgatgaaaac gctggtgaca
     6781 gatttaaatg tgaaactatg cccgaaaaca cgtattaaat acttggtcgg caatgatgat
     6841 gaaacgcata taagtgaagt tgttttggaa catgttgaaa gtggtgatac gcatgcagtt
     6901 aaatttgacg atgtcattat tagtcatggt tttgatcgtt gtaatacatt attgagtgaa
     6961 acatcttcaa agctagatat gcatgatgat tgtcgtgtta aaggatttgg taatacgaca
     7021 actagtatac ctggtatcta tgcgtgtgga gatattgttt atcatgatgc aaaatcacat
     7081 ctaattgcaa gtgcatttag tgatggtgca aatgcagcga atcttgctaa aacatatatc
     7141 caaccagatg cgaatgcaga gggttatgtt tcaagtcatc atgaagtgtt taaagaagcg
     7201 aataagacta ttgtaaataa acatttatac taggttgata tattgtgtga tgaaaagtgg
     7261 ggtaaataat ggttaaagtg acatatgata ttccgacttg cgaggattat tgcgcattaa
     7321 ggattaatgc aggtatgagt ccaaagacgc gcgaagcagc tgaaaaagga ttacctaatg
     7381 ccttatttac agtaaccttg tatgataaag ataggttaat tggtatgggt agagtgattg
     7441 gcgatggagg aactgttttt caaattgttg atattgcagt tttgaaaagt taccaaggtc
     7501 aaggttacgg cagtcaaatt atggaacata ttatgaagta tattaaaaat gtggctgtcg
     7561 aaagtaccta cgtaagtctg atcgcagact acccggcgga taaattgtat gcaaaatttg
     7621 gatttatgcc taccgaacca gattcaggcg gtatgtatat caaatactaa aaatgaaaaa
     7681 tcgtaccgat aaacatactg agttttctac acagcttaac ttagtatgtt tttgtttttc
     7741 taagtaatga tattaaaaaa atgtgtgttt tatggtaaag ttcaagttga tatgatatag
     7801 aagtttcgac tttgtaacta agggagttag aacaatgaaa atcgatacat taagacaagc
     7861 tataatatat tattttgttg cacttgtgtt atcagcttta atgatctatt tatttttaac
     7921 aaacattgag agagttattt tgtatagttg tgtattcaca gtcgtttatt gggcaatata
     7981 tgcctttgat gtatatagaa agagaaatta aattttattt ttagacacag gctgggacat
     8041 aaatcaatat tctatgctct acgaagttat attggcagta tttgattagt gttgcaagta
     8101 tgataggggc ggagaataca tgattcccta aaatgtatta aagtaaagtg ctatttgatg
     8161 aagtatcaga aattttataa accaaaagaa attaaaaaag acatctcgca taacgggatg
     8221 tctttttcat gaatactatt tatctacacc gaaatcgata acaatgcggc ctttattttc
     8281 atgtttaatt actttgttaa gttgttctgg aagttcatca aatgtaataa cttgcttgat
     8341 ctcatgtaaa ttttcaggct ttaaatcttt tgcgagacga cgccaaacgc gctgtcttaa
     8401 tttcatagca gtaaatactg agtcaattcc taaaatgttt acgcctctta aaatgtgagg
     8461 gaatacagaa ttagtataag tattaccggc agtcatacca ataactgcaa ttgatccact
     8521 atgatttaaa cgctttgtaa cataattaat accttcacca ccaacagggt ctacacaagc
     8581 ttgccaagtt gatgatgcga gtggcttttt atgatcatct tcaacaggaa gtcgatcgat
     8641 aacttctttg gcaccaagtt caagtaattg attgctaaca tcttgtttac ctgtacttgc
     8701 gataacttta taaccaagtt cattaagcat gagtactgct aaagtaccga caccacctga
     8761 agcaccgcga acgagtacag gaccatcttc aatattcatt ccaacttttt caagtctttc
     8821 aattgctaaa ccggcagtat aaccagctgt gccatatatc attgattctt ctaatgttaa
     8881 agtatcagga agcttgacaa tccattctga ttttacacgc gcatattcac taaaaccgcc
     8941 ataatggctg acacctaggt cataactcgt tacaattact tgttctcctt tttcaaagcc
     9001 tggtgcttcg gattcaacaa ttgtaccagc taaatctatt cctggaatca taggatacga
     9061 ttttacgact gcattatgat tttgagtcgc taaagcatct ttataattta taccagagta
     9121 atgtacttta atcagcacat ctcctttagg taaatcagta gtcgataatt gtttgaaagt
     9181 tggtgtcact ttaccgctct catctttatc aattacaaac gctttaaatg tttctgtcac
     9241 aacgatacat cctctctttt tctcttttaa aaatacggcc attttactgg aataaatgta
     9301 tcatttattg atgttacaca caatcaatac aatttgtaat tgatttgtgt taatgtattt
     9361 ttaaatagtt ttggcagaaa aatacataag taatattttg attcgattac tgtttattgg
     9421 aggatttata tggctgggat aatcaaagaa atttcaaaac aagatgctag tgaattagtt
     9481 gaattagcaa ctagaacatt ttatgacacg tttggttctt actatgatga caatgatttt
     9541 gatcaatttt ttaaagataa ttatactgtg gaaaaattta cacaagagat taaccatgta
     9601 gattcatttc attattttta tcaagaagat ggtgcgaatg ttggttatat aaagatgaat
     9661 attaatagtg ctcaaactga agaaatgggg gagacctatt tagaagtaca gcgcatatac
     9721 tttttgaaag actttcaagg tggtggaaga ggttcacaat tgatagcatt ggccgaaaaa
     9781 attgcacaag aacataataa acataaaatt tggctaggag tttgggagca taatcctcgt
     9841 gctcaagcat tttataaacg tcatggattt aaagtggttg gtgaacatca ttttcaaaca
     9901 ggtgatgtaa cggatacaga tttaattatg gaaaaagagt tatagcagta acttctgttg
     9961 tcgataaaaa gaaattgctg ttgtaatcat tgaatatgta tagaaatgta aaatgtgtaa
    10021 taaaattgtt gagtaaaatg attaaatgta taaaaaacat aaaattcgca aaaattagat
    10081 aataatgatt aaaatttagg gtataataaa aagaaccata gatgatagtt gttgatattg
    10141 ggggaaataa aacaccatat tattgttgac gtctttggta acaatccatg aaatgataca
    10201 catggtagat atgtatttca agcttattca atgaatatcg aattatagga ggagatatgt
    10261 atgaaaaaat tagttacagg gttattagca ttatcattat ttttagctgc atgtggtcaa
    10321 gatagtgacc aacaaaaaga cagtaataaa gaaaaagatg ataaagcgaa aactgaacaa
    10381 caagataaaa aaacaaatga ttcatctaaa gataagaaag acaataaaga tgatagtaaa
    10441 gacgtaaaca aagataataa agataatagt gcaaacgata accagcaaca atctaattca
    10501 aatgcaacaa acaatgacca aaatcaaacg aataataacc agtcaaacag tggacaaacg
    10561 actaacaatc aaaaatcaag ttacgttgca ccatattatg gacaaaacgc agcgccagtg
    10621 gctcgtcaaa tttatccatt taatggtaat aaatcacaag cattacaaca attgcctaat
    10681 ttccaaacag ctttaaatgc agctaacaac gaagcaaata aatttggtaa tggtcataaa
    10741 gtttataatg attattcaat tgaagaacat aatggtaact ataagtatgt ttttagtttt
    10801 aaagacccaa acgtaaatgg aaaatattca attgtaacgg ttgattatac tggacaagca
    10861 atggttactg atccaaacta ccaacaataa tgttaatgta attatgatgc aagttaaaaa
    10921 attaaaacgg taaactctat aatatgaatt agggtttacc gttttttgcg tattttaaag
    10981 taaaaaggtt tacgttctat agtactttta aacctcattc acagccactg aggaatttaa
    11041 gcactattta ttagatgtca ttttagacaa tataagttgt gaaatagatt ctaatggttg
    11101 ttctttctgt tgtatttgtg gatatttaat cactggtatt gcctgggaaa ttaaattatt
    11161 aattaataat tgtcggtctg tataccaaat ttttgataga tgacgccaaa tatcttcgtt
    11221 aacaccttga gactcaatat attgactgtg atttggagtg tataatgcta ctgttttgtc
    11281 tatagccatc gcatcaaata ttatatttga gtaatcagtg ataacgacat cagacattag
    11341 aattaagtct tgagcactga gatactttgg tgcaacaagc gcctcttctg gtaacatcgt
    11401 ttcatcaaca ccctgaacca caacatgata ggctttgaat aaagcatctg ataatggtaa
    11461 ttgttgcgca ctcactaatc caataggtgc atataataaa actggttttt gatcatttat
    11521 tttaaacgat ttcttatact gttgttgagt agtactttcg ttacgctttt gtagtaaata
    11581 ttggtgttta ggattaccat aagccaatac atttgcttgg tgactaggaa aagccgtttg
    11641 ataaaagtgg ctaatatcat cagcagaatg tataacataa tcgaattgca accagcgatt
    11701 atacctttta gcacgataaa aaggtgtttc tatattttta ataggttctt tactatctaa
    11761 aaataattgc attattggtg tgccttgatg taattgaatt aaagtaccgt ttggttgcaa
    11821 cgtttcaggt atatcatttt caactaaaac gacacgtgcg ctgttaatca attcgtcagc
    11881 tttttcagac ttaggtgata tgaaatgtgg tccgcgacga tcgtcagtaa caaaataaca
    11941 ggcagtcatt ggattatgtt taacgaagta agtaaataaa tattttgaat tacctatata
    12001 ttgataatga tcaccgataa aaacgatgtg atcttttaaa acgtttggtt gcttcgtata
    12061 caggccgagg ttttgtaatt gtttgttatc agcacgttgc tttgctattt tatataacca
    12121 tttaggtagc ataaacttgg tagaatacaa ccaactatct ggccaattaa gttgttctga
    12181 atcctcgata aaaaggttgc gaaaaggtat aggatgatga tcgatataag ccattttatt
    12241 caaataaaac tgagacatca tatcaaaatc ttcaatagtt ttaatagttt cccatattga
    12301 agcacttgtt gctaaagggg ctttaaaatc aaaaacgaca tgaccatcga ttgataaatc
    12361 acctttcaga agtgtcaata aagctaaatc gaaaatcgtt tctacagagt aataatgtat
    12421 taaatcaaga gtctgttgaa tcgcatatat caggttagga taataattat tgttttgagc
    12481 ccaatcggca atgttgatcg atgattgacc gaaatagtga caatcatttt tgtattgtcc
    12541 atccacagta taatcaacca cgatagactt aggatataat tgaaccgcat ctaacataga
    12601 ttgataaatc attatctcat tggcatatgg aacggcatgt gtataacctt cagactgagc
    12661 tttcattaat tgagattcaa acttttgcat attagaaaca tttatctgtt taatcattgc
    12721 tataacttcc atttctcatc gtagtatctt ccttatttta gcacggtctt atgtaaaatt
    12781 catgaatagt ataagttact tcattacatt aagtacaatt cattttgatg aacagattta
    12841 aaatgaaaaa taaaacaatt aaataggaaa gtgatattgt taatagtctg aaaaaatggt
    12901 atattttaat tgtgaaaaaa atcacattaa tctcactgtt ctgtaaaatg ttatcgataa
    12961 caaaatgcta tagccagagc atgattttaa tgaagcaatt acactactac tttatggaaa
    13021 gaagagtcag cctatgttag taaatacgtt taacccattt gataatttat tattatcgtc
    13081 tttaattgca gcaataccga ttgtactgtt tttactttgt ttaactgttt ttaaaatgaa
    13141 aggtatttat gcagccatta caacacttgt tgtaacatta ctaattgcaa taccattttt
    13201 caaattgcca gttggtatcg cttctggtgc agtagtcgaa ggtttcttcc aaggcatcat
    13261 tccgattggc tatatcgtta tgatggcggt attgttatat aaaattactg ttgaatctgg
    13321 acaattttta acaattcaag atagtattac aaatatttca caagaccaac gtattcaagt
    13381 tttacttatt ggatttgcat tcaacgcatt tttagaaggt gcagcaggat ttggtgtacc
    13441 aattgcaatt tgtgcacttt tattaacaca attaggattt aatccgttaa aagctgcgat
    13501 gttatgttta gtcgcaaatg cagcgtctgg tgcttttggt gcgattggta tccctgtagg
    13561 tgttgtagaa acgttgaaat tacctggaga tgtttcagta ttaggtgttt ctcaatcagc
    13621 aactttaaca ttggcaatca taaatttcat tattccattc ttacttatct ttattattga
    13681 tggttttaga ggtgttaaag aaacattacc agcaatttta gtagtttcaa tcacttatac
    13741 acttacgcaa ggattattaa ctgttttcag cggacctgaa ttagcagata ttattccgcc
    13801 gttattaaca atgttagcat tagcagtatt ttctaaaaaa ttccaaccaa aacacattta
    13861 tcgtgttaat aaagatgaag aaattgaacc tgcaaaagca cattctgcaa aagcagtatt
    13921 acatgcatgg agtccattca ttgtattaac agtcattgta atgatttgga gtgcgccatt
    13981 ctttaaaaac ttattcttac caaatggtgc tttatcatca ttagtattta aattcaactt
    14041 acctggaaca gtcagcgaag ttacgcataa accattagta ttgactttaa atattattgg
    14101 acaaacaggt acagctattt tattaactat tattattaca attttaatgt ctaaaaaggt
    14161 taactttaaa gatgcaggta gattattcgg tgttacattt aaagaattat ggttaccagt
    14221 tcttacaatt tgtttcatct tagcaatttc taaaatcaca acttatggtg gtttaagtgc
    14281 ggcaatgggt caaggtattg ctaaagcagg taatgtcttc ccagttctat caccaatctt
    14341 aggttggata ggtgtgttta tgacaggatc agttgtaaat aacaactcat tatttgcacc
    14401 aattcaagct tctgttgcac aacaaattgg aacaagtggt tcacttcttg tatctgctaa
    14461 tacagttggt ggtgtagcgg caaaattgat ttcaccacaa tctattgcaa ttgcaactgc
    14521 agcagtaaaa caagttggta aggaatcaga attattaaaa atgacattga aatacagtgt
    14581 atgtttacta atattcatct gtatttggac tttcatcttg tcattattat aaataaacgt
    14641 atttcaaaat ataaatatac agagggtgag atgttttcta acatctcatc tttttttaat
    14701 ggatcattga tgaaagaagt ttgacattat aataatggta gcgctttatg ttaaaatgaa
    14761 gagtgagtaa tcagcaatca aattaaattg gttgataggt gttaaggttt gtggttttgt
    14821 ctttgtgcta tcgcacataa agtatataat taaagtagtt tcgttattat aaaatattaa
    14881 tatacatagt agatagtaat agagcatcac catgagaacc tgttgagaca cttattgatt
    14941 caaagtggta ttaatatgtc gtatttctcg aacgttccat tattcatttt aaaaaggggg
    15001 actgtatttg ttatgacaac acaacatagc aaaacagatg tcatcttaat tggtggcggt
    15061 attatgagtg caacattagg aacattactt aaagaattat cacctgagaa aaatattaaa
    15121 gtgtttgaaa aattagcaca acctggcgaa gagagttcaa atgtatggaa taatgccggt
    15181 acagggcatt cagcactttg cgagttgaac tatacaaaag aaggtaagga tggcacagtt
    15241 gattgtagta aagcaattaa gataaatgag cagtaccaaa tttcaaagca gttttgggca
    15301 tatttagtta aaacaggaca attagataac ccagatcgct ttattcaagc ggtgccacac
    15361 atgagttttg tcattggcga agataacgta gcttttataa aaagtcgtgt tgcaacgtta
    15421 aagaaaaatg ttttattcga aaaaatgaaa ttatcgcaag atgaagaaga aatgaaatct
    15481 tgggtaccgt taatgattga agggcgtaag tctgatgaac caattgcttt aacttatgat
    15541 gaaactggta cagatgttaa ctttggtgcg ttaactgcaa agttatttga taatttagaa
    15601 caacgtggtg tggaaattca atataagcaa aatgtattag acatcaagaa acagaaatct
    15661 ggagcatggc tagttaaagt taaagattta gaaactaacg aaacgacaac atatgaatct
    15721 gattttgtat ttattggtgc tggcggtgcg agtttaccat tactccaaaa gactggaatt
    15781 aaacaatcaa aacatattgg tggtttcccg gtaagtggat tattcctgcg ctgtacaaat
    15841 caagaagtga ttgatcgtca tcatgctaaa gtgtacggaa aagcagcagt gggtgcgcca
    15901 ccaatgtcag tgccacactt agatacacgt tttgtagacg gcaagcgttc attgttattt
    15961 ggtccatttg caggtttctc acctaaattt ttaaaaacag gttcacatat ggatttaatt
    16021 aaatcggtta aaccaaataa tatcgtgacg atgttatctg caggtatcaa agaaatgagt
    16081 cttacgaagt atttagtgtc acaattgatg ttatctaatg atgagcgtat ggatgattta
    16141 agagtctttt tcccaaatgc taaaaatgaa gattgggaag tgattacagc agggcaacgt
    16201 gtccaagtaa tcaaggatac ggaggattct aaaggtaact tacaatttgg taccgaagtc
    16261 attacgtcag atgatggcac attagctgca ttacttggtg catcacctgg tgcgtcaaca
    16321 gctgtagata ttatgtttga tgttttacag cgatgctatc gtgatgaatt caaaggatgg
    16381 gaaccaaaga ttaaagaaat ggtgccgtca tttggttatc gcttaacaga tcatgaggat
    16441 ttatatcaca aaattaatga agaagtaact aagtatttac aagttaaata ataaacgaaa
    16501 cggtaatgtc tttttaaatg tgatagacat taccgttttt tagtggttct ttttattatc
    16561 agtgctttat atttagaagc ttatcttgat tgaagtcatt taaaagtttt ggagaattta
    16621 tacctgtctg tatcgtttta aaccaacaat atttaagata ataaagacta tgataaagat
    16681 cattagaatt agtaaatcga accatatatt actccaacct tgccccttaa tcataacatt
    16741 ggttaaagca tcaccagcat atctgagagg gaagatatag ccaatgtttc ctagccaatc
    16801 aggcatattt tcaagtggaa aaattcctga aaagaatacc tgaggtacag caactagtgg
    16861 aataaattga atcatttgaa actcagaatt agcaaacgtt gaaatgaaaa ttcccataga
    16921 taatgctgta atagctaaac aaatattaat caaaattaca taccataagc tacctgctaa
    16981 attaatgttc aataaatata ttgaaaatag gactatgatt aatgtttgaa tgattgcgaa
    17041 tataccatag ccaatcagat aaccaagaac gatttcactt cgacgaattg aagttgataa
    17101 tacacgttct aacgtacctg ttgtacgttc acgtaataaa gctatacctg atattagaaa
    17161 tacaaataaa aatacaaaga agcccattag tattggaaac attttatcga aataagtact
    17221 atcagaatta ccgtataaat aggaatgtga aatcttaata tcttgtttgt ttggttcttt
    17281 catggtcgga ggtgcttttt caattagatg ctttatactg cttattttat cttgttgtat
    17341 agctttgcca atcatttgtt tgatcatatt agatttgtta gggtcttcat ttgtataaat
    17401 gacatataat gtttgatttt cttggtaaat ataaccatca agatgattat tctcgatttt
    17461 agatttgata ttttcatgag aatggaacgt ttgagttttt atgtcggacg atggcaattg
    17521 ttttgttata tttgatgaaa cagatgtatc gataccgatt tttaaatcgt tttcatcacc
    17581 gttaaaaata aaatacataa gcgtcaatat taaaattggt gctagaaaca ttagcgcgag
    17641 tgtacgttta tcacgtaaca tttcttttaa tactcgtcta acaatagcta aaattttcat
    17701 gattgacaaa cctcctcagc tttaataaat accgcttcaa ttgtatctac attaaatctt
    17761 tgttttaaat tggttggcgt atctatagca aatattcttc cgtcgacaat aagtccaact
    17821 ttatcacaac gttccgcctc atccataaca tgtgttgtga tgacgacact ctttccgtct
    17881 ttggtcattt gcttgaactg ttgccatatt tgttggcgca atttagggtc aatgcctaca
    17941 gttggttcat ctaatatgat taattccggt tcggccaaca atgttattgc taaagataat
    18001 cggcgtttca tgcctcctga aaactgtgca acttttttat ttaacgcttg ctctaaattc
    18061 actagaacga tatgtttttc aatattatct tttaatatat ttcctttcag acctgacaaa
    18121 cgaccgtaaa actctaggtt ttcatgagca gttaagtttt cgtataatgc atcagtttgc
    18181 cccatatagc ctaatcgatt taaagctttg cgattaggca tcttatgagc gaaaatttct
    18241 gcagatccag aatctatctt ttccatacct agtaaacact ttattgttgt tgttttacca
    18301 gaaccactag gacctattaa ccctagcagt tcacctttat tgattgacat actaatttcg
    18361 tttaatacgt gatgcttgtt atacgttttt acaactttat ccaacttcgc gatagttgct
    18421 tccatgataa cccctccaaa attgagatac atttcacttt atttatatat catcataagc
    18481 gtatattaag attgcgaata aaatattaat agatagtgaa catatttgtt tttagtgttc
    18541 agaaaaaagg tggggcatat ggatcaaaca gcttttacgg atagtcgtgt aacgaaaacg
    18601 ataaatcgca tacatgatgg aatggtaaat ctattaaaaa cgaaggcata taatgatata
    18661 actgttaaag atatatgtga aaagagtcaa ataagtcgga caacctttta tacacattac
    18721 aaaactaaag atgattttat atacagctat cttaatacat tgttaaaaga ag
//
LOCUS       DATTQF010000038        18479 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.038, whole genome shotgun sequence.
ACCESSION   DATTQF010000038 DATTQF010000000
VERSION     DATTQF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.038"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(84..809)
                     /locus_tag="VB241_001155"
     CDS             complement(84..809)
                     /locus_tag="VB241_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244458.1"
                     /translation="MNNKLIYTSYDGDNILLIDSFIKLVIDFKYIPINPTKSLGYYIS
                     TSIHDNDKGECLRDCLSLEMICDELWVFIDNNKYIPEGVRLEIASWLKYKSSPVKYIS
                     IPSLLENSSINDDLFLDFDDSNILKEKEISEPVPKKSELRPVNCINILPEHHKYIDWI
                     KYHLFYNKFVPLDYLSIKPYIYFDNIEHYKSELSLLNERCNNISVMPYYVSEDNFNLS
                     FSECKIPKYIKKDWAITTMENKN"
     gene            complement(975..1733)
                     /locus_tag="VB241_001156"
     CDS             complement(975..1733)
                     /locus_tag="VB241_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP1244459.1"
                     /translation="MLEFKAGKINKHVLYSDILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFFSNQSPDQLYE"
     gene            complement(1876..3444)
                     /locus_tag="VB241_001157"
     CDS             complement(1876..3444)
                     /locus_tag="VB241_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEP1244460.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSVFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGSGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            3786..4871
                     /locus_tag="VB241_001158"
     CDS             3786..4871
                     /locus_tag="VB241_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP1244461.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(5067..5837)
                     /gene="fabI"
                     /locus_tag="VB241_001159"
     CDS             complement(5067..5837)
                     /gene="fabI"
                     /locus_tag="VB241_001159"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEP1244462.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(6115..7959)
                     /locus_tag="VB241_001160"
     CDS             complement(6115..7959)
                     /locus_tag="VB241_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HEP1244463.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDLDIVVCATNDDDINRKVAKLAKTHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(7969..9354)
                     /gene="mgtE"
                     /locus_tag="VB241_001161"
     CDS             complement(7969..9354)
                     /gene="mgtE"
                     /locus_tag="VB241_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEP1244464.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQDHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(9375..10229)
                     /locus_tag="VB241_001162"
     CDS             complement(9375..10229)
                     /locus_tag="VB241_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP1244465.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFVIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTFGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(10226..11035)
                     /locus_tag="VB241_001163"
     CDS             complement(10226..11035)
                     /locus_tag="VB241_001163"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP1244466.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(11052..11687)
                     /locus_tag="VB241_001164"
     CDS             complement(11052..11687)
                     /locus_tag="VB241_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP1244467.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(11704..12051)
                     /locus_tag="VB241_001165"
     CDS             complement(11704..12051)
                     /locus_tag="VB241_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244468.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            12237..12830
                     /locus_tag="VB241_001166"
     CDS             12237..12830
                     /locus_tag="VB241_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP1244469.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LEKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            12934..13299
                     /locus_tag="VB241_001167"
     CDS             12934..13299
                     /locus_tag="VB241_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HEP1244470.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMAFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            13322..14128
                     /gene="yjbH"
                     /locus_tag="VB241_001168"
     CDS             13322..14128
                     /gene="yjbH"
                     /locus_tag="VB241_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HEP1244471.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRLRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(14588..16396)
                     /gene="pepF"
                     /locus_tag="VB241_001169"
     CDS             complement(14588..16396)
                     /gene="pepF"
                     /locus_tag="VB241_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP1244472.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDENAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(16444..17430)
                     /locus_tag="VB241_001170"
     CDS             complement(16444..17430)
                     /locus_tag="VB241_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP1244473.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNESIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIIMFQQIQYLGGHKYVAEKKNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDHEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVSDNLNYFKLKKFIVALESKTAIIEKLINNYLNISSDRGNDVQILF"
     gene            complement(17551..18270)
                     /gene="mecA"
                     /locus_tag="VB241_001171"
     CDS             complement(17551..18270)
                     /gene="mecA"
                     /locus_tag="VB241_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP1244474.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         6216 a         3300 c         2500 g         6463 t
ORIGIN      
        1 gctcaaactt tttcattaag cataaatttt taatactctt ttttaaatca atataatgat
       61 tgaaatttaa tgatatatcc atgttaattt ttattctcca ttgttgttat agcccaatct
      121 ttcttaatat atttaggtat tttgcattct gaaaaagata aattaaaatt atcttcactc
      181 acataatatg gcataacaga aatgttatta catcgttcat tcaatagcga taactcactt
      241 ttataatgtt ctatattatc aaaataaata tatggcttta tacttaaata atccaatggt
      301 acgaatttgt tataaaataa atgatacttt atccagtcta tatatttatg gtgttccggt
      361 agtatattaa tacaatttac aggtcttaac tcactttttt tcggtactgg ttcggaaatt
      421 tccttttctt taagtatatt tgaatcatca aagtctaaaa ataaatcgtc atttatactc
      481 gaattctcta ataaagaagg tatgcttata tacttaactg gagaggattt atattttaac
      541 catgaggcta tttctaaccg aactccttct ggaatatatt tattattgtc tatgaatacc
      601 caaagttcat cacaaatcat ttccaaagac aagcagtctc ttaaacactc acctttgtca
      661 ttgtcatgaa tcgatgtaga tatataatat cctaatgatt tagttggatt tattggaata
      721 tatttaaaat caataacaag tttaataaaa ctatctatca atagtatgtt atcaccatca
      781 taagaggtat atataagctt attattcact gtaaattccc cccttcacta ttctatacat
      841 ttcaccttct tgaagctctt tatccgttgt tattgcagga atatgctttt ctcttaatat
      901 aatacctaag tgccctaaaa ttgcccctct tttgaagata aaaccactac aataatcagt
      961 tatataagaa catattactc atataattga tccggtgact gattactaaa aaaatacaat
     1021 aatatatctg acagcaatgg tttccaatct ttccattggt gaccaccttc aaattcatcg
     1081 taataataag ttatattata tttcttaatt tgttcagcta attctctatt cggtgttaag
     1141 aaatcggcac gcttaccatt tgttggtaaa gtgaaatctt tttcatctag accaatgaca
     1201 tgccaaattg tcaattgttc tttattagca cattgattta gcttatctaa tactttttca
     1261 tctgaatgtg gacttaacat tgctacacga ctaaaaatcg ttggataggt caacgccgtt
     1321 aataaggcaa tactacccgc taaactatct cctactaata accttgcatt acctactttc
     1381 agtgtagaaa acgtcgagtc aataaatggc aatatttctt taccgactga ttgaatagtt
     1441 aaatgagaac gacttccttg tggatgaaat tcttctctac gcttatcaac gtcttcataa
     1501 tggaatccga caatgatcgc atcatcaata cgcgcttctt tgattaacga ttcatatgta
     1561 cgttgtattc tcccgaaacg taaaaaatct aatccgtcaa agcaaagaat gacattatat
     1621 ttaacaagtt gattataaga ttctggcaaa taaatactta gtgtgacatc tctatttaaa
     1681 atatcactat ataaaacatg tttattaatc tttccagctt taaattccaa cataaaaaag
     1741 tgccccctag taattgtatt attattgtac caagagacac tatattttaa aattcaaatt
     1801 tctgagcacc taaaattctc aatacttttt cttttttatc aaatgatcac tacatatacc
     1861 ataactaaaa gttgactact ttttggcacg tgcttgtttt aaacgttctg gatatgtctt
     1921 caaccaaaag acagcattag gtaatttatt cggatcaggt tgataaactg cattataacc
     1981 gctgcctaaa cgtttctttt gaatttcata atcttttaaa gcatttacgg caggcttatg
     2041 taaaatccaa atcgcaatga tatttaacca agccattaga cctacaccta aatcaccgaa
     2101 tgcccatgct acatcagctg ttttaactgc accgtaaaat gtagcgaaca aaataatcac
     2161 acgagcaata ttaataaata ccgatgaaac ttgattattt tgattacgcg ttaaataagc
     2221 aacatttgtt tctgtaatgt agtagtacga caaaattgta gtaaatgcaa agaagaataa
     2281 agcaaatgca ataaagtacg aacctacgcc agagaaagta ggatcaaatt gataaccact
     2341 gccatggaac gctttatcaa tgccggcttg tgcatacatc gcagtacctg aataatcttt
     2401 acctgttgca ttttcaacat aaatcccacc gtctttaatt aaatgcggtg tgccattcgc
     2461 attaaccgta ccatcagtca cattatatgt accagaaata agtataatca gagcagttgc
     2521 agtacataca aataatgtat caatataaac tgaaaatgct tgtactagac cttgtttact
     2581 tggatgtgat acttctgctg ccgctgctgc gtgtggacct gtaccttgac cagcctcatt
     2641 tgagtataat ccacgtttaa caccaatctc tatcattgcg ccaacgatac caccaaaagc
     2701 agattgtaat ccaaatgctg atttgaaaat taatgcaaat aacgctggca cttcttgtat
     2761 attgatacaa atgataatga cagccattag tatgtaaata attgccataa atggtacaac
     2821 ggctgttgca acattggcaa tactacgtac accaccaaaa ataattaatc ccaaaataac
     2881 tacaacaata ccacccatta accattgtgg aacatgaatc gcattgtgca tagaacttgc
     2941 tatagcgtta gattgcacac caggaagcaa tagacctact gagataatcg taacaatagc
     3001 aaagataatt ccgtaaattt taccaaattt accaccaata ccatattcta tataatacgc
     3061 tggtccacca cggtattcat tattttcaac tctcttgaat atttgaccaa gtgtagattc
     3121 aataaaagca ctacttgcac ctaaaaatgc agtaatccac atccaaaata ctgcaccagg
     3181 tcctcctata aatattgcag tagatacacc gacaatatta cctgtaccaa ccctgcctgc
     3241 taaagacatg gctatcgctt gaaaacttga aataccattt ggagacttct ctccttgaaa
     3301 cattaatcga atcatttctt taaaatgtct aacttgaaaa aatcgcatac gcaatgtaaa
     3361 tagcacacca gtaattagca aaccataaac taatggctta ctccaaacaa cctcatttaa
     3421 aaaggttact aatttttcaa tcataagatt ccccctattg tttaaacata aaaatattat
     3481 acgattaagc acgagacact tcaatatatt tttcagaata ttcttataaa tattaatatg
     3541 atcatttcat tatttaaaca cgtttaatat taaaataagt tattcatata aattggtact
     3601 gtttgatcca tttgcagact tacgagtcat tgaaaagtct cacaaactat tgaaagtaaa
     3661 tatcttaaaa tagaaagtaa atggttaatt taagtatatt taaaaaatat taaccttttt
     3721 aagcactgct atttaggata tactaaataa taactaagtt tagaaaaata ggaggaacat
     3781 cgtttatgtt aaacaaggtt tggttccgaa ctggcatcgc tctgattatg ctgttcattc
     3841 tcatcaaact atttatggaa gtgcacgaag tatttactcc aatagctaca atcatcggtt
     3901 ctgtatttct tccattttta attagtggtt ttttatttta tatctgtcta ccttttcaaa
     3961 acttacttga aaaagtcggt tttccaagat gggctagtat aacaacaatc atgttagctt
     4021 tgtttgctat tatcggctta attgttgcgt ttgttgcacc aatcatcatt tcaaatatca
     4081 acaatttaat tagtcaaaca cctgccttac aaaaagaagc agagcaaatt attaaattcg
     4141 cactagctca aatggataaa ttacctgaag atgtaacaag tagaattaca aatatggtta
     4201 aatcaatggg tgatggcgtt actaatattt tatctaattc attacagtac attacatcat
     4261 taatttcaac catcttctta ttgattatgg ttccattctt cttaatttat atgttaaaag
     4321 accatgaaaa atttatccca gctgttgcta aatttttcaa aggtgaacgt aaagtatttt
     4381 tcgtagattt attaactgat ttaaacttta cattaaaatc ttacattcaa ggtcaagtaa
     4441 ctgtcagtgt tattttaggt atattcttat atatcggtta ttcaattatt gacttaccat
     4501 atattccttt attagtacta tttgctggtg tcgcaaactt aattcctttc ttaggttcat
     4561 ggttatcatt tgcacctgca gcaatactcg gtattataga tagtcctact acatttatat
     4621 gggtttgtat cattacatta attgcacacc aacttgaagg taatatcatt acaccaaatg
     4681 taatgggtaa atctttaagt atccatcctt taacaattat cgttgttatt ttagcagcag
     4741 gagatttagg tggctttaca ttagttctga ttgcagtgcc attatatgct gtacttaaaa
     4801 cggttgttag taatattttt aaataccgcc aacgcattat tgataaagca aacagtaatg
     4861 ttaaggacta attctgtgga tgtcttttaa taatataaga tactatcgcc tcaaaagttg
     4921 aaactacagc ttttgaggcg atttttttgt gcataaaaaa atcagtcaga tgaaatatca
     4981 aataattttc catcagtccg attattataa aagcaaaaaa gctttgctca catatataat
     5041 aacgtgaaca aagctgttga atgatattat ttaattgcgt ggaatccgct atctacatga
     5101 atattttcac ctgtaacgcc acttgataaa tcacttaata agtaagccgc tgttttacct
     5161 acttctactt gatcaacatt acgttttaaa ggtgcacgct cttcgatttc tttaagaatt
     5221 gtattgaaac cacccacacc ttttgcactt aatgtacgga ttggaccagc tgaaattgca
     5281 ttaacgcgaa tattatctgg acctaagtct aatgctaaat atttaacatt tgcttctaaa
     5341 ctcgctttag caacacccat cacattatag ttttgaactg cgaattcgcc acctaaatat
     5401 gttgttgcaa caatgctacc accttctggc attaattttt tagcttcatg agcaacgata
     5461 gttagtgagt aagaactaat gtcttgagct aacaagaaac cttcacgtga agtttcagaa
     5521 aaacgtccgc gtaagtcttc catattagca aatgcgattg aatgatatac accatcgata
     5581 ttgccaacat ctttaccaat ttgctcaaaa ccattaataa cttcttcatc gctttgaaca
     5641 tcaatttgat ataagtgcgc ttctggttga tttaattgtt ctaataattt ttcaagctct
     5701 ttacggctac gttctttacg gtaagtaaat actaatttag cacctaattg atctaaaact
     5761 ttagcgacac caaaagcaat actacgctta ttagcgattc ccatgatgac atatgttttg
     5821 ttttcaagat ttaacatgta agataactcc ttataataat taatttaacg gatagaatat
     5881 ctaatcccag ttttaactaa ctttttattc gtcaatacat tttaacacta ttatgacttt
     5941 ttaataaagt tatttaaacg tattacaacg ataaatgttt gatttagtcc tttctgtatc
     6001 aaacttgatt tacgtaaaaa gcatgtattt atcaaaatca tatacataca agcattttaa
     6061 aatcattaat tttacgtcgt actaaaatac gtctgcattt taatatcatt attgttaaaa
     6121 ataaaattct aactcttgct tcaattcatc aacgtattct ttagcaccgg taacaattaa
     6181 gcgatcacca tatcgcaatt gtgtatctcc atgcggaaca atcgactcat tattacggat
     6241 aatacgcacg aagatgatgt ctcctccgaa tgggaaatta cgtaattgaa tattttcata
     6301 tttataattt aacatttgaa tttcatatag tgacgtttca acattactta ataaattcaa
     6361 catgttaggt gtttcaatta aaccttttaa taagatttta ttacttaagt agctactgaa
     6421 aatttcaata cctgaatcaa ctaactctgt atcgtccgtt gtgctttcaa gtctgcaaat
     6481 gacacgctca acttgatgtg ttttggctaa tttagcaact tttcggttaa tatcatcgtc
     6541 attcgtggca caaacaacta tgtctaggtc aaacagacct agtcgttcta aaacatcttg
     6601 ttcataatca gcaatttcta tcatcgtgat atcatccgac aattgacgac gatcactcaa
     6661 gtctttgcga taatataatg tcacgtcata taactgagat gttaaatttt gcgctatagg
     6721 aatcgttaat tgatttttac caattaaact aacttcaatt ttacggttaa actcatctgg
     6781 aactggaaac agttttttga aaataatcgg aacgaataca catgtaatga ctgcgcttaa
     6841 aattaatata cctgacgttt cagctgaaat agcatttaat ctttctgaaa ttttggctgc
     6901 agctatcacg agcgataatg ttgatgttaa taaaaatgct gatgcaatcg ttgttttcat
     6961 atcaaaccaa cgtcgaataa acatgactgg aattaatttt gaaatgataa atgcaacgat
     7021 taaaatcggt atgataatta gtaatttcgg ttctttaatt aatgaaggta tgtttaaatc
     7081 tacaccaacc attatgaaga aaataggaat aaaaaacccg taaccaaatg agtctaactt
     7141 ttcaaccatt tcttcatctg gatttaataa tgaaacaacg acacctgcta agaatgcacc
     7201 taatatattt tctgcgccaa ctccctctgc tagggctact aataatatta ttaatgcaaa
     7261 caccgcacga ataccgattt gcgtcgtacc atccatcaat ttttgtaaaa atgacattct
     7321 tttaaattga acacctaaaa tatatgaaat tgctgtgaaa acaactaata tacctattaa
     7381 ccatattgta ctgccgcctt gaccattgat tgcaccatag accgttaata aaatcatagt
     7441 tactaaatcc gcaagcactg ctactaatag gataaattgc cctatagttg ttctcataat
     7501 attcatttct tttaaagttg gaacaactac gcctaaggaa atagttgaaa tgataatgac
     7561 cattaataac acatcatcca ctaatccaag ccatttaaat acatacgcta aaagaatcga
     7621 aataatcata ataaatgcaa atacagttaa cgctagatta agatgccctg gaattgagga
     7681 ttcatcatca ttttgtcctt gacgtgcgcg tttatctttt ttaaaagctt taaaatcaat
     7741 ttctaaacca cttaaaaaca ttaaaaagat aaagcctaac gttgataaaa tatttagaat
     7801 tgaatccctt tctactatat ttagaaatga atttccaatc acaattccca tcaaaatttc
     7861 tgcaacaaca actggcaaga aattaatatt taatcgattg acaattattg gagttaaaaa
     7921 cgctgctaaa acaacaataa ctaaagataa aaactccata actcctcctt aaattaagta
     7981 agccataaat gatgtagcta aaccaaaata aatcaacata ctaataatat cattaattgt
     8041 tgtaataaat ggtccactag ccactgctgg atcgatattc aatttattca tcaataatgg
     8101 aatcatcgaa cctacaaacg tccccaccgt catcgcacaa gttaaacttc ctgcaacaat
     8161 taatgctaaa agtggctgat gatatattgc aacaataatt gtaaataata ttgttgaaca
     8221 tacaacaccc gataatacac cacttcccgc ttctcttaat gcaattctaa atttactttg
     8281 ctcattaatt tcccctgtcg taatgttacg aactgaaacg gctaaagatt gtgtacctga
     8341 atttcctgac ataccactaa taataggaat aaacgctgcg agtagcgcta cattttctaa
     8401 tgttttttcg aatctcccta aaattgtcgc agtaatcatt cctaaaaatg ttaaaataat
     8461 caaccatggt aaacgcttta atgctgtttt aatgattgaa tcattagtcg aatcgatatc
     8521 tgaaaccccg gctaaacgag agtagtcttc actagcctct tcatccataa cgtctaaaat
     8581 atcatcaatc gtgatgatcc ccagcaaatg gtcttggtaa tctataacag gtacagccat
     8641 gaaatcatag tctctcataa cttgagcaac atcttcttgg tcgtctgcta cattcacact
     8701 aatgacacgt tcgttcataa tatcttcaat atacgcatca ttttcagcta caattaaatc
     8761 tcttagcgat aaaacaccta ctaatttacc atcatcatca acaacaaaga taacatatat
     8821 tgtttctgcg tctggcgctt gtgctttgac caataataac gcttctttaa caggcgtatg
     8881 cgctttaagt gataaatact ccgtcgtcat aataccgccg gccgtatcct catcatagtg
     8941 aagtaatgct ttgatttcat tcgcgtcatc tttattcatc aatgttaaaa gactagcaac
     9001 ttttggttta gtcaactcat ttaaaatatc tactgcatta tcgtatgaca tttcttctaa
     9061 tatgtgacta gcgtatgtcg cattcatctt atcaaatagc aactcatatt catcgtcatc
     9121 aatatctaat tgatcaaaga aatttgcaac ctcttcaggt gataaatatt gaaaaatctt
     9181 ttgtctattt tcatcggtag tatcttcaaa atactcactt tgttcatatg tgtgtaatgc
     9241 tagaaattca tctctaaatt gatcaatatc atcattttct aagtattgat ctaataatgt
     9301 ctcatcatat aattcctctt gaacacgctc tttttcatct gtgttcattg acatacttac
     9361 accccctgtg tgaattagag ttgattgaat aatttaagta attgcttata atcaattgta
     9421 atataaatat tgttcttatt gattggatga acaaaatata tttgcgtaca ttgcagtact
     9481 tgaccatgaa ttttgtcatg aaaaccatca tacaaagaat ctcccacaat tggatgccca
     9541 atatgttgaa aatgtacacg aatttgatgt gtacgtcccg tatgcaaatg aactttacac
     9601 aaactatatt tatcattttg atttattact tcataagatg ttttagcgta tttaccatcc
     9661 gaggcaactt ctctagttat aatactatcc tttgaccgtc taatattagc ttcaataata
     9721 ccaaatgtat gggttttacc atatacaagg caagtatata tttttttcaa gtttacttta
     9781 gaaaataaat gatggatgtg tccatattta gcgaatatca caataccagt tgtattacga
     9841 tctagacgcg taacaatatg tgggttaata ttttcaccat gttcttgaca atgatatagt
     9901 acttgttcga ttaaactttc atgaggatgt tctctcgaag gcgtacaatt ttgattgttt
     9961 ggtttagtaa ctatgatgac aaaagcatct tcatacaata cttctagctt acgagcataa
    10021 ggtattaaat taacactcgg tatttctcgc ggcaaatgaa tttctaatat atcatttggc
    10081 attaattgct tacgcactgt gactggttca tcattaacaa ttaaagcgcc attattttta
    10141 atggcgctca ctgtcttctt agaaaaatca tgtcgtgcta aaaaagtttt aacagtttct
    10201 tgttgtgata tatgatactt aaatttcatc gttcttcatc acttgatatg aaagaatcgt
    10261 gtacacgttt ccagaatggg aatggtctaa aacgtgcaaa cctcactttt tcatttgcta
    10321 cacggtattg tattgaatta acatttttat gtttgatact aacatgatct atcgtcattc
    10381 taatggtatc atgattaact ggtgatatta aacatgtatg atgctttggt aatacaagtg
    10441 gtgatcctac cgttctaaac acacgattat ttattgaggc aatttctgta atttgcattg
    10501 cttcaagtga aggatgtata agtgcgccac ctagcgcttt gttataagcc gttgaacccg
    10561 aaggtgttga tacacataat ccatcgcctc taaatcgctc aaagtgtttc cctcttaagt
    10621 taacatccac aacaagtgtt gagccatttt cagttttcat cgttgcttca tttaatgcta
    10681 aataccttgt ttcatagccg ttgtcgttgt atctcataat aatttcaagc aatggatatt
    10741 caatgacctg aaactctgaa ttattaattt cgatgattaa tttttcaact tcatgaggta
    10801 accaatccgc ataaaatcct aaatgacctg tatgaacacc aacaaatgcc acttttgata
    10861 acatgtggct atactgatgg aatgcttgta gtaatgtacc atcaccacca actgaaataa
    10921 caatttcagg attttcacta tcctcaatca tgcgaaaatc tttcatatag ttcatcattt
    10981 tatgctttaa ggcattagac ttggagtcac ctttagttaa aattgtatag cgcatgattt
    11041 ataacacctc gttaatcatt ttcatgtttt ttagaacgtt tttgcgtgta atatttttga
    11101 gcttcctgaa tttcatcttt aatttcagac atctcttcat caagtaaata cgctgcttct
    11161 gccgctcttt ccaaacgatg ttgaatttca tccggataag caccatcata tttatatcgt
    11221 aaagtatgtt caatcgttgc ccagaaattc attgctaatg tacgaatctg aatctcagcc
    11281 aatataaact tttggccttg taatgtttcg attggatatt caataatgac atgatacgag
    11341 cggtaaccac tttctttagt gttacgaata taatctcgtt cttcaattac tttaaaatct
    11401 tttctttgtc ttaaaatatt gacgacaaca tcaatatctt caacaaattg gcacatcatt
    11461 cttaaaccag cgatatcgta catttcttct cttaacctat caaatggtat ttgtcgtttg
    11521 tttgccttat ctataatact agcgattggt ttaacacgac cagtaacaaa ttctattggc
    11581 gacgcttgtt caccaacttc atattgtttg cgcatgcctt taagtttcac tttcaactca
    11641 tcaaccgctt gcttataagg tgttaagaac tgatcccatt gattcatagt gcttcacccc
    11701 gctttattca atgtcgaatg tttcttcaac ggttgcaaca aattccttac cataattatt
    11761 atttcctttt atgttgtcta aaatataagt aagttcttgg tggaattcag tcaattctag
    11821 ttcatcattg ataataattt ttttagtcat gttttcatgc aacatcgtcc atgtttcttg
    11881 tacgaaaatg agataagcat acgctttacc atcatctaat gtatacgcaa aggcataatt
    11941 atcactatca acgagcattt gtcctacttc agataatcct ttaatagaat cttctgtata
    12001 acaataaagt atgtcatctt taattttaat ttcattaata tataaacgca tgcttaatcc
    12061 tcctcttatt ccataacatt ttaacacaat tcaataatac aaaacactta cgaacactat
    12121 ttttaatggt caaattaaca ttgataatag ataaaggaaa ttacttttaa aattcaactt
    12181 tgtcattcat aatatagtta agataaattg aatttgatac gaggtgtata catatcatgg
    12241 caacaaatca tgaaatagaa tttaaacaaa tgataactgc atcaatctat aataaattgc
    12301 aagagaaata ttttaaagac agtgtgcttt ttaagcaagt caattactat attgatacac
    12361 ccgatttcaa attaaaggaa catcgctctg cactacgcat aagagttaaa gacaatcaat
    12421 atgaaatgac tttaaagaca ccagcaaagg ttggtctatt agaatacaac tatatcgtcg
    12481 acattaaacc agagatgaac ttgattattt caaacgacaa tcttccagat gacattcgtc
    12541 aaattatagt tgaacagttt ggtgtaaagg atacaacttt atcaatactt ggcgcattaa
    12601 cgacatatcg ccaagaaact aaatataaag gtgatttact tgttttagat aaaagtgagt
    12661 atttcgacac tacggattat gagttagagt tcgaagttaa agactataat caaggtttag
    12721 aaaagttcca atctttatta aatgaactaa acctcgaaca tcatcaacct ttaaataaag
    12781 ttcaacgttt ttttaagaaa aaagaaacac tttcaaacaa tataaattaa attggcaaat
    12841 atgcatattt ctttcaaatg tttaaaatca tgttatatta gttatatatt aacttaagtt
    12901 cgtaacagta aatttttgaa aatgggtgat taaatgacaa caacaccata tgacatcatt
    12961 ggtaaagaag cgttatacga tatgattgat tatttttaca cccttgtaga aaaggatgaa
    13021 cgacttaatc acctgtttcc aggagatttt gcagaaacaa gtcgtaaaca aaaacaattt
    13081 ttaacacagt ttttgggcgg tccaaacatt tataccgaag aacacggaca tcctatgcta
    13141 agaaaaagac atatggcgtt tacaatcact gaatttgaaa gagatgcatg gttagaaaat
    13201 atgcagacag caattaatcg cgctgcgttt ccacaaggtg ttggagatta tttatttgag
    13261 cgcttaagat taactgctaa tcatatggtg aattcctaaa attaattgta ggtgaataaa
    13321 catggctgga gaattacgaa taatggaaaa taagagtcgt gaagatatta atctatcacc
    13381 tgtaagtaaa atcgaaattt attcattttt cgatccattt agctccgatt gcttcaaatt
    13441 atcagcaatc ttatccaaat taagaattga atataatcaa tatatacgta tcagacatat
    13501 attaaatcct tcgttaaaag tattaacgaa atgccaagct caaagtacat ccaactttga
    13561 taacatcgcc ctagcttata aagcagctga gttacaaggt cgtttacgtg ccgaacgatt
    13621 tatacattta atgcaaaatg aaatcatacc taaaagagat attattacag aatcaatgat
    13681 ttgtgactgt attcaaaatg caggtattga tttagaagta tttaaagacg acttacaaaa
    13741 aagtaaatta accgaaagct tgaaaattga tttgcatatt gcaagagaaa tggaaatcga
    13801 acaagcccct tctctcgttt tctttagtga agatgttcat gaagaaggtt taaaagtcga
    13861 aggattatac ccatatcaca tctatactta tataattaat gaattgatgg gtaaacctat
    13921 cgaaaagaat cttcctccta aattagaaac ttatatacag caacaacaac ttgtaacgat
    13981 ggaagaatta cttactattt atgaatggcc agaaaaactt ttaaacaaag agttaaagaa
    14041 gttagctatt caacaaaaaa ttgaaaaact taaatatcca gatggcgatt tctggaaatc
    14101 taaaatgcct aaaatcaaat caaaataaat aaattaaaga cctcgtacac cataaatgta
    14161 acgaggtctt tttattgact cttaattttg caacattaaa ttattagaac gtaatgttac
    14221 gcatcgatct aaatatatta gtgatgtttt tcgaatttaa ccagttcgaa aattatacaa
    14281 attatgaatt tggtacataa tagacatata ttaatcgtta atgtataaaa agtgcatatt
    14341 taatacattt ttatacaact tcacttccct cttattaaaa aagaacataa aaaaacgtcg
    14401 tgtctgaata cacgacgcta aacaaagggg atgggagaaa tttttcactt caaacaaagg
    14461 ggtatgtttg ttatgtgatt aatttcatgt tcataatata acacgctatc tctcccattt
    14521 caacctttta atagtactta atttaaattg tcacaatctg ttcataatgt aagcgaatac
    14581 aacagtgcta agctttcatt aatttttcaa aagcgttcaa tttttgttca aaaacttcac
    14641 aagcttgttc aattggttca ggtgttgtca tatctacacc agcatttttt aatatctcaa
    14701 ttggataatt tgagctacct tttttcaaga attcattaat atatctatct actgctggct
    14761 taccttctgt taaaatttga tgacttaagc tttgagctgc actgtaacca gttgcgtatt
    14821 gatatacata ataattcata tagaagtgtg gaatacgtga ccattcctta ctaatatctt
    14881 catcagtttc tacagaatca ccgaagtata atttatttaa tttggcatat tcttcattca
    14941 ttctagttgg cgttaatggt tcacctgctt cttcaatggc atgaatttta tgctcaaatt
    15001 ctgcgaacat tgtttgtcgg aataatgtag ctctgaaacg ttctaattct tggtttaata
    15061 ataataagcg tttttcatca tcaagatgtt tatccatata atcacttaaa agtgcttcgt
    15121 tacaagttga tgctacttca gcgacaaaaa tagtgtagtc actagaattt gacggttgga
    15181 attttctact gaagtaacta tgtgctgaat gtccaaattc atgaactaat gtgtataagt
    15241 ctgaaatagt attagaccag ttaagtagaa taaatggatt agttaaatgt gcacctgatg
    15301 aatagccacc tgaacgttta cctttattct catagacatc gacccaacga ttgtttaagc
    15361 cttctttaac tacatttaaa tattcttcac ccattggttc taaagctttt agcatccact
    15421 ctttagcttc ttcataaggc atttcaaact taatatcttt aattaatggt gtatataaat
    15481 catacatttt caagtcatct aaacctagca attctttgcg caattcagta tatctatgta
    15541 gcaatggtaa atatttatgt acagttttta ctagattgtc atatacattt tctggaatat
    15601 gattattact caatgctttt tctcttgcag ttttgtaatt gtgtgtacga gcattaaata
    15661 catttttctt cacttcacct gctagcgtag cgccaagcgt attattatga gcaccatatg
    15721 ctttatatac atttctaaaa gcactttctc ttagtttgcg atcatctgat tctaaatact
    15781 taataaatgt accttgtgtt aacgggtgtg cattttcatc tttatcaatc gcatcttcaa
    15841 atactaaatc agcgttgcta aacataccgt atacatttga tggcgttgat aacgcgtcct
    15901 gcgcttctgt taataacttt tccgtttcag catctaaaat atgaggacgt ttttcattaa
    15961 tcaacttcaa atcaaatgcg aatttttgta atttatcata tgaatttaca aatgattgaa
    16021 ttttatcttc atcaatttgt aaaatctctg gcactaagaa actccatgcc gagctaaatt
    16081 taataattaa ttgatgtgct cttgactcca taccagtata cttgtcgttc gttgtatctt
    16141 ggtcttgttt tagatgcgcg tatacatata ctttttctaa tttagtacct aatgtatctt
    16201 ctaattctaa cgcattgtat aatgtctcag cactatcacc aatgtgtcct ttaaattgtt
    16261 cttctttgcc taactcattt tcaacttctt taaatgcagc ctcaaaagct tcatcatctt
    16321 tgaaaattgt tgttaagtcc catgtatatt caggatattt acgttcctgt tcttctctcg
    16381 ataattgttg actcatatgt agtaaacctc ctaataaaat ataattgata taattttctc
    16441 attttagaac aaaatttgca cgtcattacc tctatctgaa ctaatattta aataattgtt
    16501 aataagtttt tctataattg ctgttttact ttctagagca acaataaact ttttcagttt
    16561 aaaataatta agattgtcac ttactaattt gctttttcca ttctttaacc ataaatcaac
    16621 ttgtaattgc cactcaattg gatgattttc aatataaatt tgctctggaa aaatataacc
    16681 ataattgtgc actacttcgt gatcagttaa ctgtaactga tacattgcac ttaaagtggg
    16741 ttctaaaaca gaattttgcc agcgacaata tttgatatat tgattaattg cgaactttga
    16801 taatttataa acagcatgat aatcacaagg cgcctcatta aacaactcac taattttggc
    16861 attctttttt tcagcgacat atttgtgccc gcctaaatat tgtatttgtt gaaacataat
    16921 tatttgtttt ttctctaaat tgaacgtatg aagttttcga gtgattggat taataaacat
    16981 actttgaaaa tgattgaact tcactttatt atgacaataa tcaacatcat ttaataacca
    17041 acttacttta tatcccattt tctttaaacc ttccgttcgt tgaagaatct gtttataagg
    17101 aattggcgaa tactgtagct caataacata tttattatta atcaaaatat ccggaatttg
    17161 ttttatttct tttaaaaatg gctctatctc tactttacat ccttgttgct gtatcatctg
    17221 tgctaaaatc aatttacttt tataatgttt aatcgattca ttattaaaac atttttgttc
    17281 cgctaaatgt ttatgtgcaa aatgactaat tactttgagc ccacgcttta aaataacttg
    17341 cttgccacac accggacaaa aatattgtgt ctttctcaat gcagtagttg ctaacacgcg
    17401 ttccttttct tcatttaaag ctactaacat atcttcacct caaaatatta cacgtaagtt
    17461 taactactat tactttttca gcttttgaaa caataaaaaa aagagactcc aaagagtctc
    17521 ctttcatagt gcgttgctta attaatatcg ttattcagtt gtctccggaa aataacgtcg
    17581 aacttgagct gttacgttat gactcataat tattttagca tagtcattta aataaacttc
    17641 tgttctgtct gttggataag caaattcaag caattgactg taactatcat taatgacctc
    17701 ttgatcaaca tgactatcaa aatgtacagc ataataataa gtaccatcaa ccatatataa
    17761 caaatcttca aactctgtag ttattggatt gctatgatat gcataattaa taacatcttc
    17821 taaatcgtta aatttcacaa taattgttct tgtattttta cgtgctgaag acttttgacg
    17881 tttagaacct tgagcctctt tttcttttgt tcgttgctcg aataattctt ctaattgatc
    17941 ttcaccttct aatgtttgag ctaacaattc ttgaacttgt tcatcaaatt gatcagttgc
    18001 atcatcatca gacatattca tcatatcttc atttttagat ttagaaattg tgacttcgac
    18061 acctttttca aaggcatgta cttgaatcca taatggacct tctacaacaa aatcttcttc
    18121 ttcgttaatt tcatccatca ttgaccaaaa gaattcttcg ccacgtttgc gatttgtcca
    18181 taaatcttca cgactaaatc cacgggcctc gatatcgcta tatgttataa acaattttac
    18241 agttgtatca tctactcgtt ctattctcat atcatctcac tccttacagt cgatgaatag
    18301 tatccacatt gtattaaagc atcaaacaaa atacaatata tttgtttgat ttctttctct
    18361 aaaatatttt ggtaacttat aataacatat tcgttgtagc aaaactattt aaatgattta
    18421 tacagttaaa gcattaaagc actttcttaa tttaatttta ccttatcata gcaaatgtt
//
LOCUS       DATTQF010000039        17869 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.039, whole genome shotgun sequence.
ACCESSION   DATTQF010000039 DATTQF010000000
VERSION     DATTQF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.039"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            141..944
                     /gene="coaW"
                     /locus_tag="VB241_001172"
     CDS             141..944
                     /gene="coaW"
                     /locus_tag="VB241_001172"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HEP1244475.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(1070..1741)
                     /locus_tag="VB241_001173"
     CDS             complement(1070..1741)
                     /locus_tag="VB241_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HEP1244476.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(2140..3333)
                     /locus_tag="VB241_001174"
     CDS             complement(2140..3333)
                     /locus_tag="VB241_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEP1244477.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKQATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(3333..4517)
                     /locus_tag="VB241_001175"
     CDS             complement(3333..4517)
                     /locus_tag="VB241_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEP1244478.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSDLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4827..5297
                     /locus_tag="VB241_001176"
     CDS             4827..5297
                     /locus_tag="VB241_001176"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP1244479.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(5449..5799)
                     /locus_tag="VB241_001177"
     CDS             complement(5449..5799)
                     /locus_tag="VB241_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244480.1"
                     /translation="MNILRYLLGIGFSIIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(5813..7114)
                     /locus_tag="VB241_001178"
     CDS             complement(5813..7114)
                     /locus_tag="VB241_001178"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEP1244481.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAIDSGAALEKFKTFIKNQGGDKTVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADYVVSPTLIHKIITE"
     gene            complement(7394..8056)
                     /gene="deoC"
                     /locus_tag="VB241_001179"
     CDS             complement(7394..8056)
                     /gene="deoC"
                     /locus_tag="VB241_001179"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP1244482.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSGVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            8370..9080
                     /gene="deoD"
                     /locus_tag="VB241_001180"
     CDS             8370..9080
                     /gene="deoD"
                     /locus_tag="VB241_001180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP1244483.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(9201..9644)
                     /locus_tag="VB241_001181"
     CDS             complement(9201..9644)
                     /locus_tag="VB241_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP1244484.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(9844..10257)
                     /locus_tag="VB241_001182"
     CDS             complement(9844..10257)
                     /locus_tag="VB241_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEP1244485.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFTTLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FADNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(10706..12079)
                     /locus_tag="VB241_001183"
     CDS             complement(10706..12079)
                     /locus_tag="VB241_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEP1244486.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(12752..12982)
                     /locus_tag="VB241_001184"
     CDS             complement(12752..12982)
                     /locus_tag="VB241_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244487.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(13013..13951)
                     /locus_tag="VB241_001185"
     CDS             complement(13013..13951)
                     /locus_tag="VB241_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEP1244488.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYDFE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNGRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(14387..15052)
                     /locus_tag="VB241_001186"
     CDS             complement(14387..15052)
                     /locus_tag="VB241_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1244489.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFEGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            15204..15524
                     /gene="czrA"
                     /locus_tag="VB241_001187"
     CDS             15204..15524
                     /gene="czrA"
                     /locus_tag="VB241_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HEP1244490.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            15526..16506
                     /gene="czrB"
                     /locus_tag="VB241_001188"
     CDS             15526..16506
                     /gene="czrB"
                     /locus_tag="VB241_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HEP1244491.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALIAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVIATIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHSHHAHHHAHVH"
     gene            16772..17863
                     /locus_tag="VB241_001189"
     CDS             16772..17863
                     /locus_tag="VB241_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP1244492.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLVGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLVIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVITI
                     ILFVVIHAVILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
BASE COUNT         5708 a         3323 c         2500 g         6338 t
ORIGIN      
        1 aatacattaa ttgcgatacg cttacaatgt tgaaatacaa tggcatcaaa tttgttaata
       61 attactatta ttacttgttt tttagtcatt ttactatact attatttaag gatagacatt
      121 aataatgagg tgggaatctt atgaaagttg gcattgacgc tggcggtaca ctaattaaaa
      181 tcgttcaaga gcaagataac caacgtactt ttaaaactga attaactaaa aatattgatc
      241 aagttgtgga atggttaaac caacagcaaa ttgaaaaatt atgcttaact ggaggtaatg
      301 caggtgttat tgctgaaaac atcaacattc ctgcacaaat ttttgtagag tttgatgctg
      361 catctcaagg actaggtatt ttgttaaaag agcaaggcca tgacttagca gattatattt
      421 ttgcaaatgt cggtaccggt acgtcactac attatttcga cggccaatca caacgtcgtg
      481 taggcggtat cggtacaggt ggtggcatga ttcaaggact cggttattta ctaagtcaaa
      541 tcactgatta taaacagtta actgacatgg cacaacacgg tgatcgtaac actatcgatt
      601 taaaggttag acatatttat aaagataccg aaccgcccat tcccggtgat ttaacagcag
      661 ctaattttgg tcatgtacta catcatttag atgctgattt tacacctagc aataaattag
      721 cagccgttat tggtgtcgtt ggcgaagttg ttacgacaat ggccattact gttgcacgtg
      781 aatttaaaac tgaaaatatc gtttatatcg gttcttcatt tcataataac gctttattac
      841 gcaaagttgt ggaagattac actgtgttac gcggttgcaa accgtactat gttgaaaacg
      901 gcgcattttc aggtgctatt ggtgcattat atttaggaaa atagaaaaag accatttata
      961 tgcgcgttgt tttaaataga cgctatataa atggcctttt attttatggt ctaagtgttt
     1021 tgaatacgta ttgttccatt tattatcact taattacaac tcaattcagt taaaatgtaa
     1081 gttcattaga caatttttgc gctgaaacaa ttgtaccaac cacgccagtt tttaaatcta
     1141 tcgcaacggc tttatgatca tctctaagtt catctagcca ttctccgaaa acagaacctt
     1201 caatcgtaat taaatcacac tcttcaaaat cttcatctcg aacttttaac gctctgtttt
     1261 taatactcca tacaggcata atatgcggct ttttttcgtc gctatcattt tcactaatga
     1321 gaataaactg cttactacct tttttagtta agccgtatac ttcatcatat ttcgctacat
     1381 cttggacaaa ttctttgatt ctaattttat ctaattccgt catcacttct tgtgtaacag
     1441 ctacaacgtt tagtagatgc ccttcttctt ccattgtttg attcataata atatggtctt
     1501 gttcatcaaa caattcatct aattcataag tagcaaaacg atcaacgtcc atttttacaa
     1561 ctttatcaaa tggaatatca cgacttgtta aaaatgatac ggccacactt tcttctgtcc
     1621 aaacgcctac atacgcttca cttttataat ttaggcgtac tagtttttta tttttagagg
     1681 cgaaataaat acattcattt attaatatat ctctaaagaa tggatatgct ttataagaca
     1741 tgcttgaccc tcacttttta aaatatgtca tacataaaat agtaaatgac tccaacaagt
     1801 tagtcaatta gacatattat gaattcaatt taatgtcatg tttttcattt taaaaattca
     1861 atagcattaa attttacgta tacacaaatt cggtctataa cacgaaacat atctagttac
     1921 taaaaatttc aaaacgaaaa cctttagtat acctttatca acaaaatggc aactcttata
     1981 acatagtgaa ataaaagtcg ccatttaata ttagtattac aaagtattgc catatcataa
     2041 atttaattac tgttgactag aatcgaaaaa agcttgttac aaacgcatat tcagtcaact
     2101 actggcaaca caacattgta gcgccttatg ttccgaactc taatagtgtt caagttcagc
     2161 taaaaatgcg cgttcattct ccagtactgt ttcttttgaa tcaccatgcc aaatacagcc
     2221 aaaccttgat ttaactttat cttcaccgta ccaatcacca tcataataag ataacgggta
     2281 taaactacct tctttgacat atttcaaaat cgtattgaag aaatgtgtgt tatcaccttt
     2341 tgaatgataa ctataaaact tttgatatcc tgaattcaac tcgttagcaa gtaataacat
     2401 gctcgttgaa ccattttgtc taaagtttaa atcaattgca taaacgttat catcctcatc
     2461 gacgagtagg tcaaaaccag ctacaccaaa gaatccttga ttcacaccgt tttccataat
     2521 ttgtctacct gcttctataa cgtgctctgg tacattagta gtattttcat ttccgttata
     2581 aaatccgtac ttatccgtta attgcgttgc cgcaccaaga tattgaatac ctagcgattc
     2641 cgagtatgca aactgaacgc aataattcgc tttttcttca atcttctgtt cgataataag
     2701 cgaattcgtt tcagcagttg cctgtttaat acgcgttatc gctttttgta aatctgcatc
     2761 atgataacaa atcataacgc catagccacc tgctgtcgga agatcatctc ccggcttaat
     2821 tacaaacggg aattcccaat tcttaatctc gttttcgaat tgctcaattt ttacaacttt
     2881 tctttttggt aaaaacttcc catttgtcca ttcaggtatt cttgctttat tatttaaagc
     2941 aacaaataac gttttatcta atgcataata ttgctgattc aagattgttt catcatgaat
     3001 atattgaaaa taaatctttt tattttcctt atgtgccaat tgtttaatca agttttcgta
     3061 agattgctga ttgttaaatg tataaattga gttcggtact tccttaccaa taacttgaaa
     3121 tagctgatgc aatttgtctg tcgcactagc ttcgtgaaca ataacaggta attgatttgc
     3181 tattaataac tccctaccag ttaaaaaatt agattgatgt tcgtccggtt tcaaccatgg
     3241 attcgatata tacgaaggtc ttgacgtata gacaacatct ttgtcatata aatcacttaa
     3301 cgttaagttc ggctcattac cattatttgt cattacttcc cattcccttt caaatgcgca
     3361 tgctcttcaa taatgtcttg ataaacgtct tgatttgtaa ttaactctaa ccccatcaac
     3421 gccattattt tagcaccttt aattaatgct tcatcaccat gtacactcgc agctgcttct
     3481 ctaaatctat gcgtatgtcc tactaaatta cgtgatccta ttttaatatg aggatgtatt
     3541 gttggcacaa catgacttac gttccctgta tccgtagagc cataaccaaa atcatcatca
     3601 ataactgctt caccaacttc ttcagcatat ttagcaaata aatcatctaa tttcggcgtt
     3661 ttaatgaatt cattcacacc gttttgaatt ggaccaaatt cataatcaca accagtctgt
     3721 atcgcagctc cacgtgcgat ttgatttact ttttctgtta atatatccaa ttctttacgc
     3781 gtcattgctc tagtataaaa acgagcatgt gtatagtctg gaataatatt agctgctttc
     3841 ccgccatcta aaatcacacc atgcacacgt tgatcttttt taatatgttg tcgtagttgt
     3901 gctacaccat taaaataact aatcatagcg tctaatgcat ttaacgcttc atctgcattt
     3961 tcagaggcat gagcactttt tccgtaaaat ttaacatcta aaacatccac tgccaaagta
     4021 tcaatcgttt tataagtttc atttcccgga tgaatcatta aggcaatgtc tatttgatca
     4081 atcacaccag ccttgacata agaagcttta gcgctaccat tttccccacc ttcttcagct
     4141 ggacatccga gaacgactac tttaccacca atttggtcaa tcacttgctt caaaccaatt
     4201 gcaccaagca cacttgcagt tccaatgata ttatgaccac aagcatgacc caatcctggc
     4261 aaagcatcgt attctgctaa aaaacctata gctggcccgt caagatccga atcatatgtc
     4321 gctataaacc cagttgcatg tccagcaatc tcggtttcaa tttcaaaatc atgttctttc
     4381 aaacgatcta ttaaagttcg agacgcaaat atttcttcat taccaagttc aggacgttca
     4441 tgaattctat gactgatttc gatataacta tatttatttg tttctatata atcgagaatt
     4501 tgttgttttt cactcatttt ttactatctc ccttttaccc tacactcatt ttaatcatcc
     4561 acaatatttt gttctttcaa aatgaattat tacttattct atcggtttta tctcttgatg
     4621 tcatctagtt tttctttatt tacaaaattt tctaataact aaaagccttc ataaatttat
     4681 aaaacagttt caaattgtaa aacataagcc ctattgttac aatttaagta ttgttagaaa
     4741 cgcaacttgc aacaaattta agtttaataa tgtaacgaat cataagcgtt ttatagtttt
     4801 gagtagttaa taggaggaaa tttaaaatga caaaaatgaa tgttgaaagt tttaatttag
     4861 atcatactaa agtggttgcc ccatttatta gattagcggg aacgatggaa ggattaaacg
     4921 gagatgtcat tcacaaatac gacattcgtt tcaaacaacc aaacaaagaa catatggata
     4981 tgcctggatt gcattcatta gaacatttaa tggctgaaaa tattagaaat catagtgaca
     5041 aagtcgttga tttaagtcct atgggttgcc aaactggttt ctatgtatca tttattaatc
     5101 atgataatta tgatgatgta ttaaatattg ttgaagcaac tttaaatgat gtgctaaatg
     5161 ctactgaggt gcctgcttgt aatgaagtac aatgtggctg ggcagcaagt cattcattag
     5221 aaggtgctaa aactatcgct caagcatttc tagacaaacg aaacgaatgg catgatgttt
     5281 tcggtacagg aaaataaatc ttagtcaatc aagttaatca gaaaagcagt cgaacaatga
     5341 tgttacaatc gccattgtcc aactgctttt tattatgctt caaagtcaaa aaatcgaaca
     5401 aatgaaaaag taaaatcttt aacatttgtc cgatttattt gagaaccact ataatttctt
     5461 aattagtccc attaacacga actgcatagg taaccttaaa tatagttgcc atgttggcaa
     5521 ttgtttatct cctaaaggta actttttaac tgccatatag atattagctg ggaatacagc
     5581 tagcaagaat agattgattg tatttttcaa gcattgagat ggtcttttaa ttaaaagtgc
     5641 aagtccaaat aatatctcaa agattcctgt aacaagaacc gctgtttttc gaagcggcaa
     5701 acatttcggt atgatatttc taaattgtcg ttctcgtgta aaatgcaata cacctattat
     5761 actaaaacct attcctaata aatatcttag tatgttcaat cagcttcaac tcctactctg
     5821 taattatttt atgaattaat gtaggcgata caacataatc agcaattgtt atgcttgaat
     5881 ctaatttttt aacaacatcg tctacatctt ggcgattact gtgaatcgtt aataatgatt
     5941 ctccttcttc tactttatca ccaatttttt tatttaaaac aataccaacc gctaaatcaa
     6001 tatcatcctc ttttgttaaa cgtcccgctc ctaacatcat cgaagcgaca cctatatcgt
     6061 tagagactaa ctcagtcaca taacctgatt ttttagcttt atattcgatt tgatattgag
     6121 cttgtggcaa acgctctgga tggtcaataa ctgttttgtc gccaccttgg tttttaataa
     6181 atgttttgaa tttttctaat gctgcacctg aatcaattgc ctcaattagc aacgctctcg
     6241 cttcttcaag cgtttcagct ttgtttgcaa gtacaaccat ttgagaacct aatgttaata
     6301 caagttctgt taaatctttc ggaccttgtc ctttcaacgt atcaattgct tcttgtaact
     6361 caagcgcatt gccaatcgca cgtccaagtg gctgattcat atcagaaata atcgccatcg
     6421 tattacgtcc cacattatta ccaatacgta ccattgcgtg cgctaatgct tcagcatctt
     6481 ctaatgtttt cataaatgca ccgctaccag tttttacatc taatacaatt gcatctgcac
     6541 cagcagcaat ctttttactc ataattgaag aggcaattaa tggtattgaa ttgacagtac
     6601 cagtaacatc ccttaaggca tataattttt tatctgcagg agttaaattt cctgattgtc
     6661 ctacaactgc cactttattt tcattaacca atttcacaaa tgttgcttca tctatttcaa
     6721 catgaaaacc atcaattgct tctaatttat caatcgtacc acctgtatga cctaatccac
     6781 gcccactcat ttttgcaaca ggaacatcta cagctgctac taatggtgct aaaaccaatg
     6841 tagttgtatc tcctacacca cctgttgagt gcttatctac tttgacacct ttaatatcac
     6901 tcaaatctat catatcacca gaattaacca tagccatcgt taatgctgca cgctcatcat
     6961 cgttcatatc ttggaaataa atcgccattg ctaaacttga cgcttgataa tcaggaatat
     7021 cccctttaac atagccgtca ataaagaaat taatttcttc cgttgttagt gtatgaccgt
     7081 cacgcttttt ctcaataatg tctatcattc tcatttttat catccttttc ttaaaaagct
     7141 taggacaaag catctgcgct ttctctagtc catttttaaa agcacaagcg aaaattatta
     7201 tagcaagcta tcgatttcat ttttaatatc acaatttaaa tgcgatgtta ttattcttaa
     7261 atagattggt tataacgtca aagtccctat taaattatct tagaatcatc atggcattta
     7321 tgatgtctta aaactgatat cgacatactt atatatggtt acgatgtccc atgcttacat
     7381 attttttata aaattagtaa tctgaatctg cttctaaacc ttgcataatt tgaacacctg
     7441 cgctcgcacc aatacgtgtc gcacctgctt caaccatttt attgaaatct tctaaattac
     7501 gtacgccacc tgatgctttt acttctacat cagcacctac tgtatctttc attaatttaa
     7561 cgtcttctgc agtcgcaccg ccacctgcaa aacctgttga agttttaacg aagtccgcac
     7621 cagccgcttt tgttaattca ctcgctttta caatttcgtc atggtccaac aataccgtct
     7681 caataatcac ttttactgtg tgacctttcg cagctttaac cactgcttca atgtcttgtt
     7741 gtacatcatc aaaacgccca tcttttaatg cgccgatgtt gatgaccatg tcaatttcat
     7801 ctgcaccatt ttgaattgca tcttctgttt caaatgcttt cgttgcagtt gtcgacgcac
     7861 ctaatgggaa ccctattacc gtacaaacga gcacacctga atcagctagt cgctctgctg
     7921 catattttac atgcgttgga ttcacacata cagatttaaa attgtatgct ttcgcttcat
     7981 cgatgatttg atcgatttgc gtacgtgttg actcaggctt caataaagtg tgatcaatca
     8041 attttgcact attcattttc tatctcctcc tttatggttg attataaaaa tacggttgta
     8101 aattagttga ttgagcgtca ggttcattta aatatcaggt tagatgttcg cttttatgta
     8161 accgcataca tatactatta cattaattca tttcccataa acaaacaata caattgaacg
     8221 tgatatcttc attatgaacg atgacttgac aacaagctaa tcgggattat atttttataa
     8281 ttctttaatt ctatagtaca aaaattcgca aaaaagggaa acaaatgtta tcttaaaatt
     8341 attaatgaat attaaggaga agataacaaa tgacaaaagg tacaccacat attcaaccaa
     8401 atggagtaaa aattgctaaa acagtattaa tgcctggcga tccgctacgc gcaaaatata
     8461 ttgctgataa ttttttagaa aatgttgaac aatttaacga tgtacgtaac atgtttggtt
     8521 acactggtac atataaaggt aaagaagttt ctgtaatggg ttctggcatg ggtattccaa
     8581 gtattggtat ttactcatat gagttataca acttctttga tgtagataca atcattcgta
     8641 tcggttcttg tggcgcatta caagaaaatg ttaacttata cgatgttatt attgcacaag
     8701 ctgcatcaac taattcaaat tatgtagatc aatacaatat tccaggtcat ttcgcgccta
     8761 tcgctgactt cgagttagta actaaagcta aaaatgtcgc tgaccaaatc ggtgctacta
     8821 cacacgtagg taacgtatta tcttctgata cattttacaa tgccgatcca acattcaatg
     8881 atgcttggaa aaaaatgggt attttaggta tcgaaatgga atcagctggt ttatatttaa
     8941 atgcgattca tgctggtaaa aaagcacttg gtattttcac agtaagtgat catattttac
     9001 gtgacgaagc tactacacct gaagaacgtc aaaattcatt tacacaaatg atggaaatcg
     9061 ctttagaaat cgcagagtaa cttatttaaa ttgagtttaa ttgctcttta acaatgcgat
     9121 taaactcaaa aagccaacac attctgggcg tatccccatt tatgtgttgg cttttattta
     9181 tattattact tatctgtaga ttagcttaag taagatttaa acatccaatt atgtttatct
     9241 actgatgttt gcatacctat aaacatatct tctgatacat catcgcctgc attaccagca
     9301 atttcgattg cgttttctaa ttgttttgag atatttgtga agtcttgtga taattcttca
     9361 accatttgtt ctgcagagta acctttcgca gcttctttaa caattgattg ctctaagcat
     9421 tcagttaatg tacctacagg gtttcctcct accgctaaaa ttctttcagc taattcgtct
     9481 acatattggc ttgcttcatt atataattct tcaaatttaa cgtgtaatga gaagaagtta
     9541 ggccctttca cataccagtg gaaattatgt agctttgtat aagctactgt ccagtttgct
     9601 acttgttgat tcaattcttt tacaacatct tgttgattac tcatatttaa tacactcctt
     9661 aaaattgtct acgtcttgct tgtttatatt aattattata ttataatgat tctaatatgt
     9721 aaagtgtttt tactctcttt tttataatta ttctaatcta tctgttaagt gttccgcttt
     9781 ttaaatatct aaattttaat tcatcatcta gtatatctat tatcaattag aaagtatgtt
     9841 ttatcaatca gtaagtttta ctttcgcatt atcatctaaa aagaacgatt tttcataatc
     9901 atttggttgg tgccatgttg ttttccacat tctatttcta ttatcagcaa acattcgata
     9961 accgaaatta cggactggtt taggtacaag ccataaacct acgcccaata atttagttcg
    10021 attaggtagt gctgtgatta gtttaataat cgcctgcgat tcaaaatata tacgatcacc
    10081 tttctgtaaa atcacactat ttttatttgc cgcttctgga tgttgtttaa aaaattgttg
    10141 tccaacttca ccttttaacg tggtaaattg ataatttctt ggtaggtcat gttgaattaa
    10201 ccagatgaca tagttataac aatatataca gtttccatca taatatacga ttggcataat
    10261 ttattcgctc cttcaatagt acttatagtc atttttccta atttaaaaac atttaaacaa
    10321 ggttgtcact tgataagtcc tcgcgacacg aaattgccat aaaatttatt tttcagtttt
    10381 gtatttattg tataatgcat tttttattta ctttaaattt ctcaatctac agtgtaatct
    10441 tatttcttat tatcaactta gcgtttagtc gtttgtctta ttcaacatac cactggaaaa
    10501 ctcaaataaa gttttaattc agtaacataa attcatttat attcatatac tgtctttatt
    10561 atttattaaa ataaaaacga gtaatctatc ataagctctt aattagctct tatggttgat
    10621 tacccgtcat tttttcttta tacggttatt caaatatgca atttttaatc tttccttact
    10681 tcttttcaaa aactaccata tattattagt catgtttgac tttgactata cgtgtgtatt
    10741 ttaaaaactg ctctgaatat ttctccttaa tttcctctaa actatcatat gtaataccta
    10801 caatatgcca attaggattg aaataataga aagaatcttt ttgtcgtgac catttaatca
    10861 tattaccgtc tttattataa cgtggtaatg ttacctcata gccttctaac acattaatat
    10921 acgtttggaa aatgtctgta tctatacgat taaaattatc aataactaaa taacttttat
    10981 cctttttagg cattaattga gtaataaatc cttcacgata gtataatgcc cctgtttcat
    11041 ttggtaaata cttgccataa agcatatctt ctgaaaaatc aggatgacct gttgttataa
    11101 caggatttgc attcgctttt tgcaataagt cattagccgc ttttaagcct tcctcttttc
    11161 gatctacaac aagtaaaata aatggcttta acgcttcttc tttttcactt tcaagcatat
    11221 ctggtttttg aaccatttca aatggagatt tcaatccatt attatcgctc atttcaataa
    11281 ttgcatcata ctgtgcttgt gacatacttg caatagcctg ttttgcattt tcttgaagga
    11341 aatataaatt tttcaattta ggatgcttat ttaatgtact taatgtaatc ggcgtaatgt
    11401 ctttctcata agacacttca atcactgtac tatttgttct accaggaatt ggtggttttt
    11461 catgaatatg ctttgatact tctccaattc caacgacaga ttgatttttc gttcgattat
    11521 aaaaaataat attgtcgcct tcttctaact gagtataaaa atgataacca ttacgtttaa
    11581 taccgttgta cgtgtgcgta taaatcgtat attggtttcc aggttcaaat tcttcagttt
    11641 cagctaaaaa gaaataacgt ggtatcttaa tttcgccttt accaagacca cttattaaat
    11701 caaactcttc tgcagtgatt tggttgaaca atgtctcttt catattactc atacgaaatt
    11761 ccaaagcttc actacgcttt aaataatctg ctgttaatgg tttcaattgt tcattaaaac
    11821 gaaactgtac acgtatttta ttttgtgcac ctgtttcaac actaataatt tcaccacatc
    11881 caagtagtcc agtatccgtc tgaacttgat aaaagatgac ttgatctcct actttagcct
    11941 ttttaaacgc tctaaatcct tgagatggat taaaatgtgc gcctgattca aataaagctg
    12001 tttgtcctac taacggttca ttatgattcc aacggttata tccacaattc aaccaaaaat
    12061 aattcgtttc tgctgtcatc ttaatactcc ttaacctgaa taaattttag aaacactatg
    12121 aattacattc ttttaatgtt tcttatgcag ttgggcgcgt atacgatcaa ctgtatcccc
    12181 tttgttcact gcgattttaa tcgcatttcc tataacattg tagcgcctag gacattaatt
    12241 tacgtcccag acccttatcg ttttcacttc taagtaagtc gaactatttt gctttacaac
    12301 aagtgcgact ctatatgcag ttggacacac acatacgagc aactgtatac cttttaatca
    12361 gttttctata ttttatttat tatatatgtc ttaatgataa aaattgttac aaacagttta
    12421 acatatttag ctacctattt tgcatataaa aaacatgctt gcatacacta tgcaataaaa
    12481 attacaaaat tatatatgat accactatgc ttgcttatct ctatagcgcc attgatacgc
    12541 atttttaaat atctatacgc ctttagaatt ttagcatgtc ttaattttca ttaaatatta
    12601 attacttcat tttatataca ccaacaaaaa ccccctcact actatgcaag tgagaggtta
    12661 tgttgatgtg ctttattttc aacaatgtca ttaacacaat catatttctg ctgttaatgc
    12721 actgtactat attcaaacgc gctatcgact attaatttga agctacaaag gtaccgcata
    12781 atggcagcac attaattaac aatgcgctaa actttaatga ggcgctgatt tgcttgtctt
    12841 taaaagtcaa catagcactg ataattccta aaatagccac tatacgtgga attgtcatgc
    12901 tcggatactg cggtatatgt aagaaagcac caagacctgc tatcacacca catactaacg
    12961 caatatatag aaattgcttc aaatcattca ctcctaaatt gttattacac tattacacat
    13021 agctaatcat caatgtgaaa tcaccttcaa agacactatc caaatcttca gaagtcaaaa
    13081 taaagtttgt accagtagtc agtttgaaaa tttcaccatc gacaatcatt tgcccttcac
    13141 cttccaacac tgtaactaaa cagaactctc taggcttcat ataatttaac gtgccagaaa
    13201 tttcccattt aaccaatgta aagaaatcat tcgatacaat gtgtgtacac ttatggttct
    13261 caataatttc actttcaggc aaaatattag gtaatggtgc attgtactga ataacgtcta
    13321 aagctttttc aatatttaac ggtctaccat tatattgatt atcttgacga ttgaaatcat
    13381 aaagtctata tgtaatgtct gacgattgca tcgtttcgta tgctagtatt cctgaagaaa
    13441 tagtatgtac tgttcctgct ggaataaaat agaattctcc aggttttact ttaatatatc
    13501 taagtatcga ctctatcgtt ccgtgttgaa catgattcgc aacttcttct ctagactctg
    13561 ctaatgtccc tataactatt tctgcatctt cttcagcatc tataatatac caacattcag
    13621 atttgccata ttgcccattt tcatgttcaa aatcataaga attatcaggg tgcacatgaa
    13681 tagaaagtga ttctcttgca tccactattt tagttagaag cggaaaatct ttgcttggga
    13741 aatcaccaaa caattcacga tgttctgacc aaatacggtc taatgtttga ccttgatatg
    13801 gtccattaat aatctcgctc gtaccatttg gatgtgctga cacacaccaa cattccccag
    13861 ttgtatcatt gtctaattga tatccaaact cacttagacg ttgaccgccc cataattttg
    13921 tttttaaaat tggttgtaaa aataatggca ttgttgcacc tccattgtga ttaagtaagc
    13981 aatagaactc tgatgttgtt gttccattat attttgattt tgttctcatt tatatcgtat
    14041 tataaacttc cacatttcaa attaactatt agtgattgta ccatatttac taacattgca
    14101 gtactgccaa ttaaagcgct tcacttaaat ttacagtact ttaacatttt taaaatttta
    14161 tagcatagag attatatctc tcttacattt gtacatattt ctctttaaat ttactcgccc
    14221 attataccaa ttaataaaca actttaatag ttgtgccata cattgttcaa attcattgta
    14281 aaacacatag acaatacgta cttattcata cttataattc atcattatca aaaaaataac
    14341 gagttacgaa aaagtaaccc gcttcaaatc atatttacta tccttattaa tccgtttcat
    14401 tttcaaattg agttaaagca tctttaatgt cctggtcacc actaataatt tgaaactctt
    14461 ggtgattaaa atgattggat gtgacaattt cttttaatac tgtcgcaaca tcttctctag
    14521 gaatttcacc tttaccttca aaatattgtg ctgcttctat ctttccagat cctgctgcat
    14581 ttgtaagtgc ccctggatgt aaaattgtat aattcaaacc tgaacgtctt aaatagtcat
    14641 cagcgtaatg tttagcaatt gtatatggct ttaaatcacc gctatcatca aaagcctgac
    14701 gtctcgaatc atatgttgaa accatgacat agtgtttaat attagcctct ttactcgcaa
    14761 tcattgattt aacagcacca tctaaatcga caataattgt tttatctgca cccgtattcc
    14821 ctccagaacc tactgaaaag ataactttat cgaatggttt aaacgtctca gttaaagtct
    14881 ctattgaatc attttcaaca tcaacaagaa ttgctttcat accttgtgat tttaacgcat
    14941 taagttgatc ggattgccta acacctgcag taaatggtac attttctttt gctaattgtt
    15001 gcactagtaa cgaacctaca ccgccattag cacctataac caaaatattc atttacaaca
    15061 ctctcctatt tattattccc tatgccatac cactttatga gatatgtaaa acttgttaca
    15121 actataaaaa tcaattgaca tactactggg aacgtattaa attaatatat gaacaaatat
    15181 tcatatgaaa ggattgtcat ataatgtcag aacaatattc agaaataaat acagatacat
    15241 tagaacgcgt aactgaaatc ttcaaggcat taggcgatta caatcgaata cgtatcatgg
    15301 aattgttatc agtcagtgaa gcaagtgttg gtcacatttc acatcaattg aatttatctc
    15361 aatcaaatgt ctcgcaccaa ttaaaattac ttaaaagtgt gcatcttgtg aaagcaaaac
    15421 gacaaggcca atcaatgatt tattcattag atgacatcca cgtagcaact atgttaaagc
    15481 aagccataca tcacgcgaat catcctaaag aaagtgggtt ataatatgtc tcattcacat
    15541 catcatcatg accatatgca tagtcatgta actacaaata ataagaaagt attgtttata
    15601 tcgtttttaa taatcggtct atatatgttt atcgaaatca tcggcggtct ccttgctaac
    15661 agcttggcat tactatctga cggtatccat atgtttagcg acacattctc attaggtgtt
    15721 gctttaatcg catttatata tgctgaaaag aatgccacaa ctacaaaaac atttggttat
    15781 aaacgtttcg aagtactcgc agcgttattt aacggtgtaa cgctttttgt aataagtatt
    15841 ttaattgttt ttgaagcgat taaacgtttc tttgttcctt ctgaagtcca atctaaagaa
    15901 atgttaatca ttagtattat tggtttaatt gtcaatatcg ttgttgcatt ctttatgttt
    15961 aaaggcggcg acacttcaca caatttaaat atgcgtggtg cttttctaca tgttatcgga
    16021 gacttattag gttcagttgg cgccattact gcagctattt taatttgggc atttggatgg
    16081 acaatcgccg atcctatcgc aagtatttta gtttccgtta ttattttaaa aagcgcttgg
    16141 ggtatcacaa aatcttcaat taacatttta atggaaggca caccaagtga tgttgatata
    16201 gatgaagtta tagctactat taaaaaggat tcacgaatac aaagtgtgca tgattgccat
    16261 gtttggacaa tttcaaatga tatgaatgca ttaagttgtc atgttgttgt agaccataca
    16321 ttgacaatga aagaatgtga attattatta gaaaacattg agcatgattt attacattta
    16381 aatattcacc atatgactat tcaattagaa acgcctaatc acaaacatga tgaatcgatt
    16441 atatgttcag gaacacatag tcattcacat agccaccatg ctcatcatca cgcgcatgta
    16501 cattaataat tttaacctac tgccattgca tcgattaaac ttttcaatgg cagtaggttt
    16561 tttatgtctt tatggcgact tatttggtct ttgatgatgc aatgtttatt aacaaatttt
    16621 caactattat ttcttacatt agtcatattt ttgacaattt actattataa ttctctaact
    16681 ttagtcactt taattaattt ttattagata ttaatatgaa aataacgtgt tttttgttat
    16741 tgtatttata tgaagaaaag gaggcaccac tttgtttact tactttaaat cagcatttaa
    16801 aaatgctaaa ccacaattat taataacatt gatatatgca cttatagcat ttgctgtaat
    16861 tgcagttgta tatttacttg ctaatttcca gcttgctaag tatgtacaaa ctattgcaat
    16921 ttattcacaa tttgggcaaa aaccaccagt agatgcatat ttaaaagtta tcgctgtatt
    16981 acttatagca gctgtagttt cactgtttgt tttagttcaa atttttatcg gaattacaaa
    17041 tgttatgaaa cgagcaatga gtcatgaaaa agtaaaattt accgatttat ttattgcatt
    17101 taaaaaaggt aattatttaa agagcgttct gatcggcctt gtatctatag caatgattat
    17161 cgttctttca cttctgacat cattactata caaattattt tctccagtat cagaaatgat
    17221 tatgaattca gtacaatcat catatgcaga tagtacacac ctggttggta ttgctattac
    17281 aactcaaagc atcataatca ttgttgtttt attgattaaa gcaattatta cttggttatt
    17341 gttaatacct attttcaact ttatgactag tttcgttgaa tcaactaacg ataaagtaaa
    17401 aacacactta gctaatggat ttaaagcaat gaaaaatggt caaaaaacat ttttcaaatt
    17461 tttcattgga atactattat taaacttagt tattatttta tttaaaacac ctgtgggtta
    17521 cttgatttca tttaatacgc aatcactctc tcaaagtgtt gcagaaaata tcataagagt
    17581 ttatacagta ataacaatca ttttattcgt tgttattcat gcagtcattt taatgggtat
    17641 cgttcaatat tacttaaaac gtggtcaaaa aattactaaa gataaagtaa aaacagctga
    17701 caagaataaa aaagttgtca ctgaaccaaa aaatacaaaa gtagaaaatg ataaagtgac
    17761 aacatctgtt gaaacgaaaa cagaaaaagc acaagattca ttaaatgata atactacaaa
    17821 aacaatgact tcagataaac cagaagacaa ccaaccaaaa taaatttta
//
LOCUS       DATTQF010000040        17790 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.040, whole genome shotgun sequence.
ACCESSION   DATTQF010000040 DATTQF010000000
VERSION     DATTQF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.040"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            58..177
                     /locus_tag="VB241_001190"
     CDS             58..177
                     /locus_tag="VB241_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244493.1"
                     /translation="MERKRKHFNEEFAKSNLSLNNEKRKFVCSKSKHSFKKSS"
     gene            complement(325..642)
                     /locus_tag="VB241_001191"
     CDS             complement(325..642)
                     /locus_tag="VB241_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HEP1244494.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(1424..2284)
                     /gene="folD"
                     /locus_tag="VB241_001192"
     CDS             complement(1424..2284)
                     /gene="folD"
                     /locus_tag="VB241_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEP1244495.1"
                     /translation="MVAKILDGKQIAKDYRQGLQNQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            2485..2967
                     /gene="purE"
                     /locus_tag="VB241_001193"
     CDS             2485..2967
                     /gene="purE"
                     /locus_tag="VB241_001193"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEP1244496.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKTM
                     VQFASEARERGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2954..4078
                     /gene="purK"
                     /locus_tag="VB241_001194"
     CDS             2954..4078
                     /gene="purK"
                     /locus_tag="VB241_001194"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP1244497.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVAVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESKDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGIKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKIAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            4082..4786
                     /locus_tag="VB241_001195"
     CDS             4082..4786
                     /locus_tag="VB241_001195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP1244498.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHIKLLNIATDKDIETLKTKALEINQVLK
                     QLMHAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4786..5049
                     /gene="purS"
                     /locus_tag="VB241_001196"
     CDS             4786..5049
                     /gene="purS"
                     /locus_tag="VB241_001196"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEP1244499.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLEDEKENA"
     gene            5051..5722
                     /gene="purQ"
                     /locus_tag="VB241_001197"
     CDS             5051..5722
                     /gene="purQ"
                     /locus_tag="VB241_001197"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HEP1244500.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPVISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQKLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            5715..7904
                     /gene="purL"
                     /locus_tag="VB241_001198"
     CDS             5715..7904
                     /gene="purL"
                     /locus_tag="VB241_001198"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEP1244501.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGIGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYEDEVYADIPVEPLADEAPVYILEGEEKNYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEALYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLADSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPIVGMVGLIENVDYLNDF
                     QPQVGDKLYVIGDTKDDFGGSQLEKLIYGKVNHEFESIDLSSEVKKGESIKTAIRQGV
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSLIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7883..9367
                     /locus_tag="VB241_001199"
     CDS             7883..9367
                     /locus_tag="VB241_001199"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEP1244502.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPLLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKIKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKM
                     LAQESPVKADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHYFDSEGNLNV"
     gene            9360..10388
                     /locus_tag="VB241_001200"
     CDS             9360..10388
                     /locus_tag="VB241_001200"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEP1244503.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIETNEMYNIFNMGIGYTVIVDEKDAPRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            10391..10957
                     /gene="purN"
                     /locus_tag="VB241_001201"
     CDS             10391..10957
                     /gene="purN"
                     /locus_tag="VB241_001201"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEP1244504.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFGSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10972..12450
                     /gene="purH"
                     /locus_tag="VB241_001202"
     CDS             10972..12450
                     /gene="purH"
                     /locus_tag="VB241_001202"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEP1244505.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTLEQAFHHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALNILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            12472..13719
                     /locus_tag="VB241_001203"
     CDS             12472..13719
                     /locus_tag="VB241_001203"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEP1244506.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCEFPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGELAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNEAIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVHDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13987..14793)
                     /locus_tag="VB241_001204"
     CDS             complement(13987..14793)
                     /locus_tag="VB241_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1244507.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIIILAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14786..16186)
                     /locus_tag="VB241_001205"
     CDS             complement(14786..16186)
                     /locus_tag="VB241_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244508.1"
                     /translation="MLKVSDLRLKYPSGQRKVFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQHLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(16201..16776)
                     /locus_tag="VB241_001206"
     CDS             complement(16201..16776)
                     /locus_tag="VB241_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1244509.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16853..16953)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(17360..17494)
                     /gene="graF"
                     /locus_tag="VB241_001207"
     CDS             complement(17360..17494)
                     /gene="graF"
                     /locus_tag="VB241_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HEP1244510.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            17646..>17790
                     /locus_tag="VB241_001208"
     CDS             17646..>17790
                     /locus_tag="VB241_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244511.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVH"
BASE COUNT         6184 a         2646 c         3348 g         5612 t
ORIGIN      
        1 gaatgaaaat aaaaaaagtt tgaaattgtc taataaattg aaaatgatgg ctagttcatt
       61 gaaaggaagc gcaaacattt taatgaggaa tttgccaaaa gtaatttatc gttaaacaat
      121 gaaaagagga aatttgtttg ttcaaaatca aaacattctt ttaaaaagag cagttaagtt
      181 ttaataattt gataggtagt cctttggtat acagaaaaaa tacaatattg gggttaaagt
      241 tgcagatgaa gttggaataa caaataaaat ggtagcaaaa catgtacgtt tgctaccatt
      301 ttatttgcag tatagtgatg agggctattt aacctcaata tacctagtgg aaattttaat
      361 tgcaccatct gaatcagtga catgatattt aatttgatat ttaccagatt tagatgtatc
      421 aatttggcca tcgactttaa ttttatcggt taaatctcca tcttctttat caaatgcact
      481 tatgccgttt agaaggttat aatcttgacc tttcttaata acgatatcat tagcgccttt
      541 aatttgtggt gtagtattcg tcgttgcatc tgcatttaaa tttggtgtta ctagtgtagc
      601 agaaacaccg agggctgata atgactgtaa tagtttattc ataatgtaac ctccgaatga
      661 taagtcattt ctgagttagc tttactatac cttaatttat attatttaca catcatacta
      721 tagtcctaat cttaccctat aaagaatgaa atttaaatat aaatttataa ataatgagga
      781 aataattatt attttgaaaa tctgccacaa ttgtaaaatt aataattcag ggataatttg
      841 aaatcaaata taactttatt cgacagtttg aacttaaatt gttgtgcatc ataacaataa
      901 gaaaagggag gcaaaaataa taaagaacca ctaatgattt attatgtagt agtattttat
      961 cattagtcac agcttctgtg ttggggactc gctgacttgc attgtctgta gaaattggga
     1021 atccaatttc tctgtgttgg ggcccatcct caacttgtac attattgtaa gctgactttt
     1081 cgtcagcttc tgtgttgggg cccacacccc aacttgcatt gcctgtagaa tttctttttg
     1141 aaattctctg tgttggggcc cctgactaga attgaaaact tgttacaagc ttaatttcat
     1201 ttaattaact attgtctata taaaattgtt gagcctagaa caatgtgaaa tgctcccttc
     1261 aaagccttca tttattcaat gtctactttg aagggagcat ttgatcgatt aatgtcccag
     1321 atttttcttg ttattcattg caattgcaaa ataatattta atttgggtta aaagagttca
     1381 ttaaaactca tttattaatt cacattacat aacttatgtg aatttacgaa tcaatacctc
     1441 gacgcatttt ttctgcaagc aaagtattat ttaataccat agtaattgtt aatggaccaa
     1501 cgccaccagg aactggtgta atagctccag caatttcttt aaccgcatca taatcaacgt
     1561 caccttttaa tttgccattt tcatctggcg tattgccaac atctataatt actgctcctt
     1621 ctttgacaac atcttttgtt actaaaccag gcttaccaac tgcactgaca atgacatcag
     1681 catcttttaa atatgatgcc atatcctttg aacgagaatg taagattgtt actgatgcat
     1741 ttttttgaag tagtaactta gaaactggtt gtccgacaat atgacttcgt ccgattacaa
     1801 ctgcattttt accttctaaa tcaatatcag catgttttaa tatttccatg atgccgagcg
     1861 gtgtgcaagg tacaaaagtt tgttcgtcga tatataattt ccctatattt attggatgga
     1921 aaccgtccac atctttatca ggattgattg cttctaatat tttctgttcg ctaacttgtt
     1981 ttggtagtgg tacttgtacc aaaataccac ttacagaatc atcattattt agtctgttta
     2041 gttcgtttaa tacttcttct tcagtagctg tttcttccaa atgtacgatt tctgaaatca
     2101 taccaatttt ttcagctgct ttcttttttg atctaacata actttgacta gcgccatcat
     2161 taccaactaa tataacggat aatttaggtg taaaaccctt ttcttttagc gcttcaactt
     2221 gattttgtaa cccctgtctg tagtctttgg caatttgttt accatctaaa attttagcaa
     2281 ccataaaaac ttctcctcct attgtcaata tcctaacttt gttagtttat aaaactaatt
     2341 tacatagcaa aagacgtaaa ttcaaacaaa aaatcaaaat aaagttcgat ttttgattga
     2401 aaaagcagaa attgcttgtt atgctatatc tataatatac aactaaagta attcacaatt
     2461 caaacttttg aaagggtgta caaagtgaaa gtagcagtca ttatgggcag ttcttccgat
     2521 tggaaaatta tgcaagagag ttgtaacatg ttggattatt ttgaaattcc gtacgaaaaa
     2581 caagtagtat ccgcacatcg tacgccaaaa acgatggttc aatttgcttc tgaagcgaga
     2641 gaaagaggta tagacattat cattgcaggc gctggcggtg cggcacattt accaggtatg
     2701 gttgcatcat taacgacgct accagttatt ggagtgccga ttgaaacaaa aagtttaaag
     2761 ggtatagatt ctttattatc aattgttcaa atgccaggag gtattccggt tgcaacgact
     2821 gcaattggtg cagcaggtgc taaaaatgca ggtatacttg cagcaagaat gttaagtatt
     2881 caaaatcctt ctttagttga aaaactaaat cagtatgaat cttcgttaat tcaaaaagtg
     2941 gaggacatgc aaaatgaact tcaataaatt aaagtttggt gcgactattg gcattattgg
     3001 tggtggtcag cttggaaaga tgatggcaca atcagctcaa aaaatgggtt ataaagtggc
     3061 tgtattggat cctgctgaag attgtccatg tagatacgtt gcacacgaat ttatacaagc
     3121 caagtatgac gatgaaaagg cactcaatca attaggacaa aaatgtgatg tgattactta
     3181 tgaatttgaa aacatttcag cccaacaatt aaaactatta tgtgaaaagt acaatattcc
     3241 gcaaggttac caagctatac agttattaca agaccgctta actgaaaaag aaacattaaa
     3301 aagtgctggt accaaggttg tcccattcat ttcagtaaaa gaatctaaag atattgacaa
     3361 agcaattgaa acattaggat atccttttat tgtaaaaact agatttggtg gctacgatgg
     3421 caaaggtcaa gttttaatta acaacgaaaa agacttacaa gaaggtatta aattaattga
     3481 aactagtgaa tgcgtagctg aaaaatattt gaatatcaag aaagaagtat cacttactgt
     3541 tacaagagga aacaacaatc aaatcactta tttcccatta caagaaaatg agcatagaaa
     3601 tcaaatactt ttcaaaacaa ttgttccagc gagaatagat aaaatagctg aggcgaaaga
     3661 gcaagttaat aaaattatcc aatcgattca tttcattgga acatttacag ttgaattttt
     3721 tatagatagt aataaccaat tgtatgtgaa cgagattgca ccaaggcctc acaattccgg
     3781 acattattca attgaggcat gtgattattc acaatttgat actcatattt tagcagttac
     3841 cggacaatca ttaccaaatt caattgaatt attaaagcct gcagtcatga tgaacttact
     3901 aggtaaagac ttagatttat tggaaaatga atttaatgaa catccagagt ggcacttaca
     3961 tatttatggt aagtctgagc gtaaagatag cagaaaaatg gggcatatga ctgtactaac
     4021 gaatgatgta aaccaaactg aacaagatat gtacgctaaa tttgagggga gtaattaaag
     4081 catgacatta ctatatgaag gaaaagcgaa gcgcattttc tcaacaaatc aagaaaatga
     4141 attaagagtt gaatataaag atgaagttac tgctggaaac ggggctaaga aagacacaat
     4201 ggcaggtaag gggcgattaa ataatcaaat tacttctatt atatttaaat atttacaaga
     4261 aaatggaata gaaagtcact ttattaagca attatctgaa acagaacaat tagttaagcc
     4321 tgtgaaaata attccattag aagtagttgt tcgtaatatt gctagcggat ctattacaaa
     4381 gcgtttaggt tttgaaaatg gtgaagtttt tagagaacca cttgtggaat ttttctataa
     4441 aaatgatgca ttaaatgatc ctttgattac agatgatcac atcaaattac tgaatattgc
     4501 gacagataaa gatattgaaa cattaaaaac gaaagcgcta gaaattaatc aagtattaaa
     4561 acaattaatg catgcgatga atttaaaatt agtagatttt aaaatcgaat ttggaaagac
     4621 tgagactggt caaattttgt tagcggatga aatatctcca gatacatgtc gaatttggga
     4681 taaagctacc aatgcaaact ttgataaaga tgtatataga aataacactg gatcactgat
     4741 tgaaacatat caaatatttt taaacaaatt ggaggattta aaataatgaa aacaattgaa
     4801 ctacatatca cattacaacc acaagtatta gatacgcaag gacaaacgct tactcgagct
     4861 gtacatgatt taggttatgc acaagtgaat gatattcgtg taggaaaagt attatatatg
     4921 acagtggatg aggttagtga tgaaaaggta cacaacatta ttacaactct aagtgaaaaa
     4981 ttgtttgcaa atacagtgat tgaagaatat agctataaag ttttagaaga tgaaaaggag
     5041 aatgcataaa atgaaatttg cggttcttgt ttttccaggt tcgaattgtg atagagacat
     5101 gtttaatgct gctattaaaa gtggtgttga agcggaatat gtagattata gagaaacatc
     5161 actaagtgga tttgatggag tacttattcc tggtggattt tcattcgggg attacttaag
     5221 atctggggca atggctagtg tagcgccggt tatttctgaa gttaaacgtc ttgcagctga
     5281 aggtaagcca gtattaggtg tttgtaatgg gtttcaaatt ttaactgaaa taggcttatt
     5341 acctggtgca ttattgcata acgattcaca tttatttatt agtagaaatg aagagttaga
     5401 aatagtgaat aatcaaacgg cttttacaaa tctttatgaa caaggtgaaa aagttatata
     5461 tcctgtagct cacggcgaag gtcattatta ttgtactgat gaaatatatc aaaaattaaa
     5521 agctaacaat caaattattc tgaaatatgt gaataatccg aacggttcat atgacgatat
     5581 tgcaggaatt gttaacgaaa aaggcaatgt gtgtggcatg atgccacatc ctgaaagagc
     5641 tttagaaacg ttgttaggta ctgatagtgg tgtgaaatta tttgaagcga tggtaaaaag
     5701 ttggagggaa caacatgtct aaatttatcg aaccaagcgt tgaagaaatt aaacttgaaa
     5761 aagtatatca agatatggga ttaagtgatc aagaatatga aaaagtttgc gatattttag
     5821 gcagacaacc taactttaca gaaacaggta tcttttctgt tatgtggagt gaacattgct
     5881 cttataaaca ttctaaaccg tttttaaaac aatttcctac gtcaggtgag catgtgctta
     5941 tgggtccagg tgaaggtgca ggggtagtcg atataggtga taatcaagcc gtagtattta
     6001 aagtagaatc tcacaatcat ccatcagcaa ttgagccata tcaaggtgct gctacaggcg
     6061 ttggtggaat cattcgtgac attgtctcta ttggggctag acctattaat ttgttaaaca
     6121 gtcttagatt tggagaatta gataataaac aaaaccaaag attacttaaa ggtgttgtaa
     6181 agggtatcgg aggttatggt aactgcattg gtattccaac aactgctgga gaaatcgaat
     6241 ttgatgaacg ttatgatggt aatccacttg ttaatgcaat gtgtgttggt gttatcaatc
     6301 acgacatgat tcaaaaaggc acagcaaaag gtataggtaa ttcggtcatt tatgttggtt
     6361 tgaaaactgg tcgagatggt attcatggtg ctacttttgc atctgaagaa ttgacggaag
     6421 aaagcgaaag taaacgacct tctgtacaaa tcggtgatcc atttgtaggt aaaaaattaa
     6481 tggaagcaac acttgaagca attacatttg atgaattagt tggtattcaa gatatgggtg
     6541 ctgctggttt aacatcttca tcttctgaaa tggcggcaaa aggtggtagt ggtttacatt
     6601 tgagattaga acaagtgcca acacgtgagc caggtatttc tccttacgaa atgatgcttt
     6661 cagaaactca agaacgtatg ttactagttg ttgaaaaagg tactgaacaa aaattcttag
     6721 atttatttga taagcacgaa ttggatagtg ctgttatagg tgaagtaaca gatacaaatc
     6781 gttttgtttt aacatatgaa gacgaagttt atgctgacat tccagttgaa ccactagctg
     6841 atgaagcacc tgtatatatt ttagaaggag aagaaaaaaa ttataatact tctaaaaatg
     6901 attatacaca catcgatgtt aaagatactt tctttaaatt acttaagcat ccgactatag
     6961 catctaaaca ctatttatat gatcaatacg atcaacaagt tggtgccaat acgataatta
     7021 agccaggact tcaagcatcg gtagtacgtg tggaaggcac aaataaggca attgcttcaa
     7081 caattgatgg tgaagcgctt tatgtatata acaatccata tgaaggtgga aagatggtag
     7141 tagctgaagc ttatcgtaat ttaattgccg tgggtgcaac accattagca atgactgact
     7201 gcttaaatta tggttctcct gaaaagaaag aaatttatca acaattagca gattcaacga
     7261 aaggtatggc agaagcttgc gacatactta agacacctgt tgtttctgga aacgtttctt
     7321 tatacaacga aacgaaaggc acttctattt tccctacacc aattgttggt atggtaggtc
     7381 tgattgaaaa cgtagactat ttaaatgatt ttcaacctca agttggagat aagttatatg
     7441 taattggtga tactaaggac gactttggtg gtagtcaact tgaaaaattg atttatggca
     7501 aagtaaatca cgaatttgaa tctatagatt tgagttcaga agttaaaaaa ggtgaatcaa
     7561 tcaagaccgc tattcgtcaa ggggtattat cacatgttca aacggttggt aaaggtggct
     7621 tactgattac cttagctaaa ctaagtgcgc attacggttt aggattaaag tctttaatag
     7681 atataacaaa tgcacaattg tttagtgaga cacaaggccg atatgttgtt tctgttaaat
     7741 caggtaaaac tttaaatatt gataatgcaa tagaaattgg gcttttaaca gatagtgata
     7801 atttcaaagt aacaacacca tatacagaga ttagtgaaaa tgtttcagat attaaacaaa
     7861 tatgggaagg ggcaattgct caatgtttaa ctactcagga ttaaacgaag aatgtggcgt
     7921 gtttggtatt tggaatcatc ctgaagcagc gcaactaaca tatatgggac ttcatagttt
     7981 gcaacatcgt ggtcaagaag gtgcaggtat agttgtttct gatcaaaatg aattaaaagg
     8041 cgagcgagga ttaggcttac taactgaagc gattaaagat gaccaaatgg aacgattaaa
     8101 aggatatcaa catgcaattg gtcacgtccg ttatgctact tcaggtaata aaggtattga
     8161 aaatattcaa ccgcttctgt atcactttta tgatatgagt gtaggtattt gtcataatgg
     8221 taacctcatt aatgctaaat cattacgtca aaatttagaa aaacaaggtg ctatcttcca
     8281 ttcgtcttca gatacagaag ttattatgca tttgattcga cgcagtaagg cgccaacttt
     8341 tgaagaagca ttaaaagaaa gtcttcgaaa aattaaaggt ggttttacat ttgcaatttt
     8401 aacaaaggat gcgttatatg gcgcagtaga tccaaatgct atcagaccac ttgttgtagg
     8461 taaaatgaaa gatgggacat acatccttgc aagtgaaaca tgtgcaattg atgtgttagg
     8521 tgcagaattc gttcaagata ttcatgcggg tgaatatgtc gtgattaatg ataaaggtat
     8581 tacagttaaa tcttatacac atcatacgac aactgcaatt tctgcgatgg aatatattta
     8641 ttttgcgaga ccagattcaa cgattgctgg taaaaatgtt catgcagtgc gtaaggcttc
     8701 aggtaaaatg ttagcacaag aaagtccggt aaaagctgat atggtcatcg gtgtacctaa
     8761 ttcatcatta tctgcagcga gcggttatgc tgaagaaatc ggtttgccat atgaaatggg
     8821 actagttaaa aatcaatatg ttgcaagaac atttattcaa ccaacgcaag agttacgtga
     8881 acaaggtgta agagttaagc tatctgcagt gaaagatatt gtagacggta aaaatattat
     8941 tcttgttgat gattccattg ttcgaggtac gacaattcga cgtattgtga aaatgttaaa
     9001 agattctggt gcaaataaag tgcatgtgcg tatagcatca ccggaattta tgttcccaag
     9061 tttttatgga atcgatgttt caactacggc agaattaatt tctgcaagca aatcacctga
     9121 agaaattaaa gattatattg gcgctgattc gttagcatat ctatctgtag atgggttaat
     9181 tgaatcaatt ggtttagatt atgacgcgcc atatagtggc ttatgtgtag aaagtttcac
     9241 tggagattat cctgcagggt tatatgatta tgaagcaaat tataaagcac atttaagtca
     9301 tcgacaaaag caatatattt ctaaaaacaa acactatttt gatagcgagg gaaatttaaa
     9361 tgtctaaagc atatgaacaa tctggtgtaa atattcatgc tggttatgaa gctgtagaaa
     9421 gaatgtctag tcatgttaaa cgtacgatgc gtaaagaagt tatcggtggt ttaggtggat
     9481 tcggtgctac atttgattta tcacaattaa atatgacagc accagtttta gtttctggaa
     9541 cagacggagt aggtacgaaa ttaaaactag ctatcgacta tggtaaacat gattcgatag
     9601 gtatcgatgc agtcgcaatg tgtgttaatg atattttaac gacaggtgca gaaccattat
     9661 attttttaga ttacattgct acaaacaaag ttgttcctga agttattgaa caaattgtta
     9721 aaggtattag tgatgcatgc gttgaaacga atactgcact tatcggtgga gagactgctg
     9781 aaatgggtga aatgtatcac gaaggcgaat atgatgtagc tggatttgct gttggagcag
     9841 ttgaaaagga tgactatgta gatggttcag aagtgaaaga gggacaagtt gttatagggc
     9901 ttgcgtcaag tggcattcat tcaaatggat acagtttagt gcgcaaatta attaatgaat
     9961 caggcattga tttggcatca aacttcgata atcgtccatt tatagatgtc tttttagaac
    10021 caactaaatt atatgttaaa cctgtacttg ctttgaaaaa agaagtttct attaaggcaa
    10081 tgaatcatat tactggtgga ggtttttatg aaaatattcc acgtgcattg ccagccggat
    10141 atgctgctag aattgatact acatcatttc caacgccaaa aatatttgat tggttacaac
    10201 aacaaggcaa tatagagaca aatgaaatgt ataacatttt taacatgggt attggctata
    10261 cggttatcgt tgatgaaaaa gatgcaccac gtgctttgaa gattttagca gaacaaaatg
    10321 tggaagccta tcaaattggt catattgtga aaaatgagtc aactgcaatt gaattgttgg
    10381 gggtataaca atggttaaaa ttgcgatttt tgcatcaggt tcaggaagta actttgaaaa
    10441 tatagttgag catgttgaat caggaaaact tgaaaatatt gaagttacgg cgctatatac
    10501 ggatcatcaa aatgcgtttt gtatagatag agcaaaaaag cacgatattc ctgtttatat
    10561 taatgaacca aaacaatttg gttcaaaagc agcgtatgaa caacatttag taacattatt
    10621 aaatgaagat aaggtagagt ggattattct agctggctac atgcgtctaa ttggtccaga
    10681 tttattagct tcatttgaag gtaaaatatt aaatatacat ccatcactat tgccgaaata
    10741 taaggggatt gacgcaatag gccaagcata tcatagtggc gatactatta ctggttcgac
    10801 agtacattat gttgatagtg gtatggatac gggagaaatt attgaacaga gacaatgtga
    10861 tattagaccg gacgattcaa aagaacaatt agaagagaaa gtaaaaaaat tggaatatga
    10921 gttatatcca agtgttattg ctaaaattgt aaaataagga gtatgtatgt gatgaagaaa
    10981 gctattttga gcgtatcaaa taaaacaggt attgtagagt ttgctaaagc gttaacgcaa
    11041 ttaaattatg aattatattc aactggtggt actaagcgta tattagatga agcaaatgta
    11101 ccagttcgtt ctgtttcaga cttaacacat tttccagaaa taatggatgg ccgtgttaaa
    11161 acattacatc cagctgttca tggtggtatt ttggctgatc gaaataaacc gcagcattta
    11221 aatgaattat cagaacaaca tatagattta attgatatgg tagtagttaa tttatatcca
    11281 ttccaacaaa ctgttgctaa ccctgatgtg acgatggacg aagcaattga aaatattgat
    11341 attggtggtc caacaatgtt acgtgctgca gctaaaaact ataaacatgt aacaacaatt
    11401 gtacatcctg cagattatca agaagtattg acgcgattaa gaaacgattc gttagatgag
    11461 tcatatagac aatcattaat gatcaaagtt tttgagcata ctgcagaata tgatgaagcg
    11521 attgtacgtt tctttaaagg ggataaagaa actttaagat atggagaaaa tccacaacaa
    11581 tcagcgtatt ttgtgagaac ttcgaatgca aagcacacga ttgcaggcgc taaacaatta
    11641 catgggaaac aattaagcta taacaatatt aaagatgcag atgctacact agctttagtt
    11701 aaaaagtttg atacacctgc tgcagttgca gttaaacaca tgaatccatg tggtgttggt
    11761 atcggtgaca cgttagaaca agcatttcat catgcgtatg aagcggatag tcaatcaata
    11821 tttggtggaa ttgttgcatt aaaccgagct gtaacacctg agttagcaga gcaattgcat
    11881 agtatctttt tggaagtcat tattgcacca aaatttacag atgaagcatt aaatatttta
    11941 aaacaaaaga agaatgtaag attattagaa attgatatga ctatagacag taacgaagaa
    12001 gagtttgttt cagtatctgg cggatattta gttcaagata aagacaatta tgtcgtgcca
    12061 aaagaagaaa tgaaagttgt tacagaagtt gcacctactg atgaacaatg ggaagcaatg
    12121 ttattaggat ggaaagttgt accatcagta aaaagtaatg caattatttt aagtaataat
    12181 aaacaaactg taggtatagg tgctggacaa atgaatcgtg tcggtgctgc taaaattgcg
    12241 ttagagagag ctattgaaat caatgatcat gtagcgttag tatctgatgg atttttccct
    12301 atgggagata cagttgaact tgcagcacaa catggtataa aggcaattat ccaaccgggt
    12361 ggttcgatta aagatcaaga ttcaattgat atggctaata aacatggtat tgcaatggta
    12421 gtcacaggca ctcgacattt taaacactaa ttttaaaggg gcgtaacata aatgaatgta
    12481 ttagtaattg gtgctggtgg acgagaacat gcacttgcat ataaacttaa tcaatcgaat
    12541 ctagttaaac aagtgtttgt cattccaggt aatgaggcaa tgacacctat agctgaagta
    12601 cacactgaaa tttcagaatc tgatcatcaa gggatactag attttgctaa acagcaaaat
    12661 gttgattggg tagttatagg tccagaacaa ccgctaattg atggattagc agacatttta
    12721 cgagcgaatg gtttcaaagt gtttggtcca aataagcaag cagctcaaat cgaaggctca
    12781 aagttatttg ctaaaaagat aatggaaaaa tataatattc caactgctga ttataaggaa
    12841 gtagagcgaa aaaaggatgc attaacatat attgaaaact gtgaattccc tgttgttgtc
    12901 aagaaagatg ggttagctgc tgggaaaggc gttattattg cagatacaat tgaagcagcc
    12961 agaagtgcta ttgagattat gtatggtgat gaagaagaag gtactgttgt gttcgaaacg
    13021 tttttagaag gtgaagagtt ctcgctaatg acatttgtta atggtgaatt agcagttcct
    13081 ttcgactgta ttgcacaaga tcataaacgc gcatttgatc atgatgaagg accaaatact
    13141 ggtggtatgg gggcttattg tccagtacca catattagtg acgatgtttt aaaacttaca
    13201 aatgaaacaa ttgcacaacc cattgcaaag gcaatgctta atgaaggtta tcaattcttc
    13261 ggtgtattat acattggtgc tattctaact aaagatggtc caaaagtaat agaatttaat
    13321 gcccgttttg gtgatcctga agctcaagta ttattaagtc gcatggaaag tgatttaatg
    13381 cagcatatta ttgatttaga tgaaggaaaa cgtactgaat tcaaatggaa aaatgaagct
    13441 attgtagggg tcatgttggc atcaaaagga tatcctgatg catatgaaaa agggcataaa
    13501 gtaagtggct ttgatttaaa tgaaaactat tttgttagtg gattaaagaa gcaaggtgat
    13561 acctttgtta cttcaggtgg tagagttata cttgccatcg gaaaaggtga caatgtacat
    13621 gatgcacagc gagacgcata cgaaaaagta tctcaaatac aaagtgacca tttattctat
    13681 cgtcatgaca ttgcgaataa agcactacaa cttaaataag tatatttaaa aatactaaga
    13741 ttagctatga acgaatctat aacgatagat ttgttcatag cttttttagt tgtagagtct
    13801 aggacattga tttctgtacc aaatttgtga ttatcatatg ttgtaataca aaagaggcgc
    13861 cacaacatgt ttggatgaac aaaataacat gtttgtggca cctcttttgt ttagtatgga
    13921 ataaatggtt ttctttttct atacaatgaa tttctaattt agtatctata caattatgga
    13981 taaaatttaa cctacacgac caagacgaac atcatcaatg cccgtgatgg gtaaggtgat
    14041 tgaacaataa tatgccaaaa taataatggc aattaaaact ataataaaga ttacatcttt
    14101 atatgagaaa ggtacgttgt aatagtaagt acgaggacca tctctaaatc ctttcgactc
    14161 catcgcaact gataattgat gtgcctttct aatgttttga cttaatagag gtatgattaa
    14221 atgcttaaat cgctttaatc ctctataatt tgctgcgtct atcatctgat agcgcatttt
    14281 taaagatctg cgaagctgta ttaaagaact aatcattaaa ggtatcatac gaatggcagc
    14341 catgaatgca taagcaactt ttgatttaac ctttaaatgt tgcattaaac tataaaaaat
    14401 catgacaacc tgagatgtga gtgcgattaa gataccgaaa aatgaaatag caatagttct
    14461 taatgataca tgtaaaccac gaactaaact ttctgttgta atatggataa atccgaattt
    14521 caaaattgta tggctaccat tcccgtataa aatcatgaac agggaagaga gtaatgcaaa
    14581 gccaatactt atagttataa aaattgctgt aattttaaac tgagtaccat taaacatcaa
    14641 taagaaaact aacattaaga tagtgatata aagcataaaa tcgaaattat gcacaaatat
    14701 aataaagaaa aatagtataa ttccaagaaa tagtttcgtt ataatgttga catcatcaac
    14761 aaatgattgc cgaaccttcc attgctcata cattcgtatc accatcacaa tctagtaacg
    14821 caccatctga aattttaagt cttcttgatg ggtaacgttc aattatttca tcgtcatgtg
    14881 taaccatgac aatactttgt cccaaattaa ttcgtttttg gaaaagtttg atcaactgga
    14941 atgtattatg gctatcaagt ccaaatgtcg gttcatctaa aaagatgata tcagctttag
    15001 aacttagtgc ggtagctacg ctaaggcgtc gtttttgacc aatagacaac tcataaggat
    15061 gttgatcttt aacattttct aaatctaaaa gttttaaaag ttgtatcgtt tcatcatcac
    15121 tttgatcttt agaaaggtga ttaaaatgaa tgttaatttc atcataaacc gaatttgtta
    15181 taaattgtaa ctctgggttt tgataaacta gatacatgtg ttttgctgca tgtttaattt
    15241 ttgttaaatg ctgattttca aaataaacat caccttgata tttaatcaat tgcataattg
    15301 attcaagcaa ggttgtttta ccactaccat ttgcccctgt aattgtaatc cactcaccta
    15361 gaccaatttc taaatctgag aatgagagca acgttgattt accgcgaata atacgtccat
    15421 ttttaaattg taataagtgt gagtttgttg ttggaaagtc aacacgactt ggtgcgaatt
    15481 cccatgcacg tggatgccac acaccatatt cactgagtaa atgaacatac ttctgtaata
    15541 tgatttcagg acattcatcg gcaatgatat ttccgttata atccatcaaa atgacgcggt
    15601 cgacatgatt ccagatgtgt ttaactttat gttcaacgat tacaacagtt tgatcttccc
    15661 aaagttcaat tagtttagtc cataaatctt ccgttgcttg aacatctaac attgctgtcg
    15721 gttcatctaa aaacaatgtt tttgattgtt gaagaatggt ttcaacaatt gccaatttct
    15781 gtttcatccc gccacttaaa tctttgatat acgtttcagg ggtaacattt aaattgacca
    15841 tatttaaagc attgataatt aacgcatcca tgtcttcacg tggtacttgt ctattttcta
    15901 aaacgaatgc aagttcttcg tatacttttg gcatacaaaa ctggctatca gggtcttgga
    15961 aaataacgcc acttaatggg tcaacgatta gttcatcata tttcataggt aattcaatta
    16021 aattaggaac aataccactt aatacattca gaagtgtact tttaccgcaa ccagaaggac
    16081 cgagtaaaag tactttttct ttgtcttgaa tagtgatatt taaatgatcg aaaactttac
    16141 gctgaccact tggatatttt aatcgtaaat cacttacttt taacactctg aatgctcctc
    16201 ttataagttg tcgtaatctt ctttagcagc tggcctaaat aattttgtta cgcctgtctt
    16261 atctaaagct tttactaaaa ggtaagatag gacgccggcg actactgcac cactaattaa
    16321 tctaaatacg atgaataatg ttaagttcca acctgcaact tcatttaaat aaccatagaa
    16381 ataatcgatc gggaaagccg cgatagctgt acaaaaacct gctaacatag ctaccataac
    16441 tgaacgagat tgatatttaa aaattgcaaa gacaagttca cacgctaaac cttgtatgaa
    16501 agcgtaaacg attgtcggaa tatcgaaacg acccataatg atagtttcgc cggcacctgc
    16561 agcaaattca gccaataaag caatacctgg ttttggaata attagatagc agacaatcgc
    16621 tgccatgaac caaaccccgt ttgttaattg ttcgaggtga aggcctgtag cttgcacacc
    16681 attgtaaaca aaccaccata aattgtaaat aactgcgaat actactgaaa taagtacggt
    16741 tactagaatt tcagatagct ttaaaccttt tgacattttt acatcctcct aataaaaaaa
    16801 cgcacaacca tccataggaa agttatgcgt tcacaatata tattagtaaa acatatgtat
    16861 agtaacactt tcctacgcta gttcaagcta gatcaggttc aaagggtttg agggcaagcc
    16921 tcatctcagt ataaaacacc cctagtgtgt gcgatttatt taattaatta tactgtaaga
    16981 cgtttataaa cttatgtcaa taggttgtct tcatgaaatt tcgtttaatt cgatttaaaa
    17041 tttataatat tagcattaga tttaaattaa agatgtagta gaaatgttag taattaaaga
    17101 tataaaaata tgtgacatgt aataatatta agttgataag tgaagaggga tacttatcaa
    17161 tcatacctct ttaacaacag tgaacaaccc gtgcataatg gcttgtgaat tattgtttgt
    17221 aaggtaaaag agggatacca gcggcgagcg caagcaaaaa agccccaaac aactaaaagt
    17281 tataaggtaa aagagggata cttgcgccga gcgcatgcat aaaatcccct aacaacgaaa
    17341 agttgtaagg ggatttaaat taatttagtg tatcttggat atcttgtttt gtttgattaa
    17401 tatcttctgt tttttcttct tttttatctt ttagtttttc ttcaacttct ttagcttttt
    17461 ctgctgcttt tttattttga ttttcatttg acatgattaa ttcctcccaa attggataat
    17521 tatttatata taaatcttac ccggttgtac tttcgttaaa cttttctaag tctatagcac
    17581 tatttattca tttatctaaa gacaacaaca ttagattaat atataatgat tttgaggtga
    17641 acataatgtc ttttcttagg aaacacgccg aaattatttt tagctattta atcggtatcg
    17701 tttcactctt cactggtctc attattttaa ttaacttgcc tttaattaaa caattaaatg
    17761 gtggtaaaaa agttgataca catgttcata
//
LOCUS       DATTQF010000041        17626 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.041, whole genome shotgun sequence.
ACCESSION   DATTQF010000041 DATTQF010000000
VERSION     DATTQF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.041"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..333)
                     /locus_tag="VB241_001209"
     CDS             complement(<1..333)
                     /locus_tag="VB241_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1244512.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     NKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLF"
     gene            complement(339..1106)
                     /locus_tag="VB241_001210"
     CDS             complement(339..1106)
                     /locus_tag="VB241_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244513.1"
                     /translation="METIKQEVRESIVKGIFAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNQKSKNYLEGLIAKNSGINFDTVGEDFKIRHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTPLLDKVIVEREDYHID
                     EEELEVLKNDGELEFIDVEHAFGISIDEDNDMIESER"
     gene            complement(1263..2798)
                     /locus_tag="VB241_001211"
     CDS             complement(1263..2798)
                     /locus_tag="VB241_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP1244514.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFATLQQHLPEMKRYADIIVVCYHGGFEKDIESGTPTEVLTGENEGYGMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDPTFTIDEDDQHIRKQLEDWLDYEITTLPYDMMINHAFEARVAPHPFTNFMNYA
                     LLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGFKLKQAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNKLMRIPQVVDFK
                     VEK"
     gene            complement(2849..4393)
                     /locus_tag="VB241_001212"
     CDS             complement(2849..4393)
                     /locus_tag="VB241_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1244515.1"
                     /translation="MKNIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQQTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQTEKVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4622..5578
                     /locus_tag="VB241_001213"
     CDS             4622..5578
                     /locus_tag="VB241_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP1244516.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5792..6565
                     /gene="phnC"
                     /locus_tag="VB241_001214"
     CDS             5792..6565
                     /gene="phnC"
                     /locus_tag="VB241_001214"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEP1244517.1"
                     /translation="MSQIKFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSKIYGRTIKEDEKLGVN"
     gene            6567..7367
                     /gene="phnE"
                     /locus_tag="VB241_001215"
     CDS             6567..7367
                     /gene="phnE"
                     /locus_tag="VB241_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEP1244518.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7364..8179
                     /gene="phnE"
                     /locus_tag="VB241_001216"
     CDS             7364..8179
                     /gene="phnE"
                     /locus_tag="VB241_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEP1244519.1"
                     /translation="MTQEIEKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIESLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIL"
     gene            complement(8310..9488)
                     /gene="deoB"
                     /locus_tag="VB241_001217"
     CDS             complement(8310..9488)
                     /gene="deoB"
                     /locus_tag="VB241_001217"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP1244520.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(9516..10178)
                     /gene="deoC"
                     /locus_tag="VB241_001218"
     CDS             complement(9516..10178)
                     /gene="deoC"
                     /locus_tag="VB241_001218"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP1244521.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKVFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAEGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            complement(10259..11611)
                     /gene="tet(38)"
                     /locus_tag="VB241_001219"
     CDS             complement(10259..11611)
                     /gene="tet(38)"
                     /locus_tag="VB241_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HEP1244522.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(11618..12325)
                     /gene="deoD"
                     /locus_tag="VB241_001220"
     CDS             complement(11618..12325)
                     /gene="deoD"
                     /locus_tag="VB241_001220"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP1244523.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIVAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            12561..13316
                     /locus_tag="VB241_001221"
     CDS             12561..13316
                     /locus_tag="VB241_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1244524.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(13418..14083)
                     /locus_tag="VB241_001222"
     CDS             complement(13418..14083)
                     /locus_tag="VB241_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1244525.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPVTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMNQNTHMN
                     QQPHMQQGHMQSSNHQMNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRQRS
                     TNK"
     gene            complement(14452..15051)
                     /locus_tag="VB241_001223"
     CDS             complement(14452..15051)
                     /locus_tag="VB241_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP1244526.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            complement(15319..16749)
                     /locus_tag="VB241_001224"
     CDS             complement(15319..16749)
                     /locus_tag="VB241_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP1244527.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAELDISWFYAGTEKFKIPSLSNIVASVIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMCIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            complement(16739..>17626)
                     /locus_tag="VB241_001225"
     CDS             complement(16739..>17626)
                     /locus_tag="VB241_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEP1244528.1"
                     /translation="KKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKGL
                     MNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIYF
                     FIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEGF
                     ASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFAE
                     WGILFGALFIIFMIYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFIL
                     GIIVFIVQYEKMERDRNEE"
BASE COUNT         5781 a         3201 c         2686 g         5958 t
ORIGIN      
        1 aaataagtag ctagttaaat tagataaata cattgcttga taatattgct tatttaatag
       61 tttcgtagat actttataat tttttgctct aaaagctata ctatgtttat cgacagaatt
      121 tattaggagc tcttcgggct gctcgctaat aaagtagcta gatttaacat taaaaataga
      181 tgtcatatca taaattttat taatttctgg catcatattt tttgtttcat attgccaaat
      241 tgcttgttca cttacattta ttttttctga taattctttt ctacttagag cattaagctt
      301 tctcacgtat tctaaattct tgccaataaa cattaacttc acctttcgct ttcaatcata
      361 tcgttatctt catcgatact gataccaaat gcatgttcta catctatgaa ttctaactct
      421 ccatcgtttt taagtacttc taactcttct tcgtcaatat gataatcttc tctttctact
      481 attactttgt ctaacaatgg tgtcaaatca tcaaccataa ttgcagaatt tgtttctgga
      541 tttggcaacc attgtttgat tgcagctaat tgctttgatt ctttatcaat acgatatgta
      601 ataattaaaa aataagaatt aatatctcta ttactgcctt cttttatatt aaataatata
      661 ctatctgcag tcatttgatg cctaatttta aaatcttctc caacagtatc aaaattaatg
      721 ccactattct tagctataag tccctctagg tagtttttac ttttttgatt cttatcagca
      781 actaaactct tccctacgct tacatttttt tcatcaaagt aatcttcatt tttcaatata
      841 aacatgatat tatttttctc ttcatcctta ttaaatataa actttaaata cttccatgat
      901 ggtccagcat tttctttaat atacttaaca aaattttcca aatgcttagc aacttgattt
      961 tcaatatgat tagatttggt gtacacatag gcatcactaa tcaccatacg tttcttgact
     1021 tcatttctct cattaacata gtccttatat cccgcgaaaa ttcctttaac aatagattct
     1081 cttacttctt gtttaattgt ttccaaatta atcacttcat aatattaagt tatataaaat
     1141 ataagccttg ttttttgtaa aatcaacatt tttcttaaag tatattggtg tttaagagtg
     1201 attagtttaa acaaaaacaa aaacttaccc gattaaaatc aagtaagttt tacatatatc
     1261 cgtcactttt caactttaaa atcaacaact tgcgggatgc gcatcaattt attatttgat
     1321 aaaaaatcaa taagtaattg tgcgccttca acttgaatat cttttacaac tggcgcgtcg
     1381 atatacatat catactgacc accgccaact gcacgataat tatttacaca aattgtatat
     1441 gtctgcttta aatcaactgc gtgaccttga atcatcatat tgctcacacg ttgtcccttt
     1501 ggtcttccaa catgaatggt ataacttacg ccaccatata tatcataatt aaagtgttgg
     1561 ggtttgggtt caaggaagtc tgcgctcaca cttacttcat catttttcac gtcaaaatat
     1621 tctgctgatc gttcaatggc ttgtttaagt ttgaaaccac ttacagctaa aactttaaat
     1681 gtatttggaa atgggtaatt gttaataaca tctcgcatcg tcacgacttg cttgaaacca
     1741 ctagcagaat caaacaaagc tgtacaggca acatctgctc cacttttttc taataaagcg
     1801 taattcataa aattagtaaa aggatgcgga gctacacgtg cctcaaatgc atgattaatc
     1861 atcatatcat atggcaatgt tgtaatttcg taatctaacc agtcctctag ctgctttcgt
     1921 atatgttggt catcttcatc aatagtaaat gtgggatcat ctataacagg aagtaattca
     1981 catgattcaa cggatacatt ttcatattca tcagtactca agacaacttt gcctacagtt
     2041 gtacctctcg taccaggttg aatcacagcc gtttgcttaa acctttcagc aatttgtcga
     2101 tgttggtgac ccgtaataaa gatatctata tctttagaaa acgcttctaa catgccatat
     2161 ccttcatttt cacccgttaa cacttcggtc ggcgtaccac tttctatatc cttttcaaat
     2221 ccaccatggt aacaaaccac aatgatatct gcatatcgct tcatttcagg taagtgttgt
     2281 tgaagtgttg caaaagcact atgaaacgta agtgactgaa tatgctctgg ttgttcccaa
     2341 tggggaataa attgtgtcgt taaacctatc acaccaaccg tttgatctcc aacctgaaaa
     2401 tactgcacac cgttatcagt caatgtacta tcattttcat aaatattagc gcacaaaact
     2461 ggataattga gtctgcgtaa agtgtctttt aagtatggta atccataatt aaattcatga
     2521 ttaccaagcg taccaaagtc gaatgccatt cgattataaa aatcaactaa aggctggcta
     2581 ctgccgctat gcgcgattaa gtaattacaa aatggtgacc cttgcaaaaa atcaccatta
     2641 tctattttaa aactttggtc atactgcctt ctgtcttgtt ctataacatg attcgctagt
     2701 aacaatccca taggctgata ttgatttcta ctcgtaaaat ctgttgggaa aatataacca
     2761 tgtacgtcac tcacgacata aaatgctatg tttgacatcc tcactcactc cttcaatcac
     2821 aaacatcttt cttatttcta ttatatattt atttgaagtc tgttgtaatc aaggttttgt
     2881 caccgagttt taaacgaatc tttgaacctt ccatactttc gagtactttg gcattgacct
     2941 taattgtggc atttccgttt tcatctgctt taactgttgg caaagtactg taacctggcg
     3001 ggttataatc gttatcttta cttgaaaatt gtccgatttg acgtcctcct tctttgtaaa
     3061 taccaactct aagattttca agtgtttgat ttgcttcaaa tccttttaaa tgtattgtca
     3121 cttcaaatgg ctcatttaca gaaacatttt gcgggatatc aaatgatact ttttcggtct
     3181 gatttggtgg cgtatgatca tctggtgtgt ttggctgagg atccgcgcct ttttcactgc
     3241 cataactacc tgctttaaat gttgttgggt cataccattt ataaccactc ggcggttgtg
     3301 accatggctc tttttcaggc tcagttgaac gctctggtcg ttcaaaatca agcaacttag
     3361 tctttgtatc taatgttagg ccactcgcct taagtgattt cccatcatta tctttagaca
     3421 tccaagctgt tatattattt aatagcttac cgttgtcttg ttctttaaaa ccatcatatg
     3481 ttttcttctt ttctccatta tcttctctca catatttggg cgaactatct tccacaagtg
     3541 atgaatcacc gataaatgct gctttacctt ttccaacttt agaaattgct acatagggac
     3601 cttctgcttt accgccccca ttataaatac cttgatctac agcatgtgac catttacttt
     3661 tcgctggcaa ttgttctggt gtatacacaa taccttttgc tttctctgga ttagtaattg
     3721 ctaatgtcga accggcatgc atagatacag atttcacacc ttcagtaata ccaaaacttt
     3781 cttttgaaga aacaatattg ctcgtattta aatcacctag tgcattatat cgaaaacgta
     3841 cgccaaagtt tgtagataac caatctgaac ttttcacacc ttgcattgca gtagaacttt
     3901 tttcttctgc attcatacct ttcgacatat cttcatacgc tccacgtcga taaccattca
     3961 ttgcctctga tgaatcaata cgatttaaat ttcggtcagc attgtaatgg tctgaaataa
     4021 agacaacatt tcccccttgt ttcacatagt taacaattgc tgcctgttct gattctttga
     4081 aaggaatgtt agcctcagga attacaaata ttttggaact tttcaaactt gcttctgtta
     4141 tgttcgaatg accatcaata gctttaacgt cataaccttg tttttgtatt gaatccgcat
     4201 aatctgaaaa tgcaccatca ctaacccaat ctgcagcacc agctgtttga ccatgagaac
     4261 gatcgaataa tactgtttgc tgttgctttg taggttgcga ttcatgcgtt atagctaaag
     4321 attgcggtaa agcacttaat gataccgttg caacaattgc agagacagtt aatgacttat
     4381 atatattttt cattttttga ggctcctttt aaaataaatt tgttcttgaa ttataggata
     4441 aatatatgtt tgaaatgggc aatttaacga aaattttaca aaatcttatc acactcttaa
     4501 agaaagttat taaaattcat ttttataaaa tactttttag catttaaatg tggtacgcta
     4561 taagtgtaat ttcattgcat acatattaca cgattaagaa tgtgaagggg acagttatca
     4621 aatgaaaaat tttaagtgtt tatttgtatt aatgttagca gtcattgttt ttgcagcagc
     4681 atgtggaaat tcaagttctt tagataatca aaagaacgct agtaatgatt cggattctaa
     4741 atcaggagga tacaaaccta aagaattaac agttcaattt gtaccttcgc aaaatgctgg
     4801 aacattagag gctaaagcaa aaccattaga aaaattacta tctaaagaat tagggattcc
     4861 agttaaagtg tctgtatcaa ctaactacaa tacaattgtt gaagctatga agtctaaaaa
     4921 agttgatgtt ggtttcttac caccaacggc atacacatta gcacatgatc aaaaagcagc
     4981 tgatttatta ttacaagcac aacgttttgg tgtaaaagaa gatggttcag caagtaaaga
     5041 acttgtagat agttataaat cagaaattct tgttaaaaaa gactcaaaaa ttaaaagctt
     5101 gaaagattta aaaggtaaga aaattgcctt acaagatgta acatcaactg ctggatatac
     5161 attcccactt gcgatgttaa aaaacgaagc aggtattaat gcaactaaag atatgaaaat
     5221 tgtgaatgtt aaaggtcatg accaagcagt tatctcatta ttaaatggag atgtagatgc
     5281 tgcagctgta tttaacgatg cacgtaatac tgtgaaaaaa gaccaaccaa atgtatttaa
     5341 agacacacga attttaaaat taacacaagc tattccgaat gacacaattt ctgtaagacc
     5401 agatatggat aaagacttcc aagagaaatt gaaaaaagct tttatagaca ttgctaaatc
     5461 aaaagaaggt cacaaaatta ttagcgaagt ttattcacat gaaggataca cagaaacgaa
     5521 agattcaaat ttcgacattg taagagagta cgaaaaatta gttaaagata tgaaataatc
     5581 attatttaac caatgaatca ttatcgaatt tggtattaaa agctttcgtt caatagatat
     5641 attctagatt aatattgaaa agctaggcgc taaactgaaa cagatataga aaggtgtcgc
     5701 tgtacatttg aaactatttg tacacagaaa cccaatgtct atgatatttc agtttacctt
     5761 ggcttttctt tattaaagaa aggtgtcaaa catgagtcaa atcaaattta aaaacgtcag
     5821 taaagtctat cctaacggtc atgtaggctt gaaaaatatt aacttaaata ttgaaaaagg
     5881 tgaatttgca gtcattgtcg gactatctgg tgctgggaaa tccacgttat taagatctgt
     5941 aaatcgtttg catgatatca cgtcaggtga aattttcatt caaggtaaat caatcactaa
     6001 agcccatggt aaagcattat tagaaatgcg ccgaaatata ggcatgattt tccaacattt
     6061 taatttagtt aaacggtcaa gtgtattacg aaatgtacta agtggacgtg taggttatca
     6121 ccctacttgg aaaatggtat taggtttatt tccaaaagaa gacaaaatta aagcaatgga
     6181 tgcactagaa cgggtcaaca tcttagataa atataatcaa cgctctgatg aattatcagg
     6241 tggccaacaa caacgtatat ctattgcacg tgcgctatgc caagaatccg aaattattct
     6301 tgcagatgaa ccagttgctt cattagaccc attaacaacg aaacaggtta tggatgattt
     6361 aagaaaaatc aaccaagaat taggcatcac aattttaatt aatttacatt ttgttgactt
     6421 agcaaaagaa tatggcacac gcatcattgg tttacgtgat ggtgaagttg tctatgatgg
     6481 tcctgcatct gaagcaacag atgacgtatt tagtaaaata tatggacgta caattaaaga
     6541 agatgaaaag ctaggagtga actaacatgc ctttagaaac acctacaaag tatgactccc
     6601 ttttaaagaa aaaggtttct ttaaaaacga gttttacctt catgttaatc attgtgctta
     6661 tcatttggag cttcatttat acggggttta attttggaga tttaatgata ggaatacctc
     6721 aaataggtga tctattcaaa caaatgattc cacctgattt cgagtattta caacaaatta
     6781 caacgccaat gttagatacc attcgaatgg ctatcgtaag tacagtatta ggtagcatcg
     6841 tttcaatacc aattgcgtta ttatgtgcta gcaatatcgt tcatcaaaag tggatttcaa
     6901 taccctcgcg ctttatttta aatatagttc gtactattcc agatttgtta ttagcagcaa
     6961 tctttgtggc tgtatttgga atcggtcaaa ttccagggat attagcattg tttattttaa
     7021 ctatctgtat tattggaaaa ttattatatg aatcattgga aacgatagat ccaggtccaa
     7081 tggaagcaat gacggctgtt ggcgctaata aaataaaatg gattgttttc ggtgttgtac
     7141 cacaagccat atcgtcattt atgtcatacg tattatatgc atttgaagta aatatacgtg
     7201 cttcagctgt gcttggatta gtcggcgctg gcggtattgg attgttttat gatcaaacac
     7261 ttggtttatt tcaatatcca aaaacagcaa cgattatttt atttacttta gttatcgtcg
     7321 tcgtcattga ttacatcagt acgaaagtga gggcacatct cgcatgacac aggaaataga
     7381 aaaatataat gttcacacaa aagcacacaa acgaaaattg attaaaagat ggcttattgc
     7441 aattgtcgtc ttagctatta tcatctgggc atttgcaggt gtaccaagtt tagaacttaa
     7501 aagtaaatca ttagaaatct taaaatccat attcagcgga ttattccatc ctgatatcag
     7561 ctatatctat ataccagatg gcgaagactt attacgtggt ttacttgaaa cctttgcgat
     7621 agccgttgta ggtactttca tcgccgcaat tatctgtatt ccattagcat ttctaggtgc
     7681 aaataatatg gtaaagctac gcccagtttc aggtgttagc aaatttattt taagtgttat
     7741 acgtgtcttc ccagaaattg taatggcact tatatttatc aaagctgttg gcccaggttc
     7801 attttcaggt gtattagctt taggtatcca ttccgtaggt atgcttggga aacttttagc
     7861 tgaagatatt gaaagtctag atttcagtgc tgtagaatca ttaaaggcca gtggtgcgaa
     7921 taagattaaa acactcgtat ttgcagtcat accacaaatt atgcctgcct ttctatcact
     7981 catactttat cgctttgaac taaacttacg ttcagcttct atactggggc taattggggc
     8041 tggtggtatc gggacaccac tcatatttgc cattcaaaca cgttcttggg accgtgtagg
     8101 tattatatta atcggtttag tactaatggt cgcaattgtc gatttaattt ccggttcaat
     8161 ccgaaaacgt attctttaac attaaatcag gatactcctg aataagaggt cctaccgtct
     8221 tacgtttctc tattataata aaaacagcag tgaagaaaac tattattata gttaacttca
     8281 ctgctgtttt tattatatct aaatttattc tattttaatt cctttaaata acttttaccg
     8341 aactctggta atgttacgtt gaaattatct gctatagttg caccgataga gctgaatgta
     8401 gtatcacttt ctagtgcatg accaccttta aatttcggac tgtacataat tactgggatg
     8461 tattctctcg tatggtccgt acctggcgct gtcgggtcat taccatggtc tgctgtaata
     8521 attactaaat cgtcttcttt taagttgcta aacagttctg gcaagcgatc atcgaaatct
     8581 ttaattgctt gtgcataacc cggtttatca cgacgatgac cgtataatgc atcaaagtct
     8641 actaagttta agaagctaat acctgtgaaa tctttcttaa caattttcat caattgatcc
     8701 ataccgtcca tgttactctt tgtacgaacc gcttctgtta caccttcacc atcataaatg
     8761 tcattaattt taccgatggc aatgacatca taaccaccgt ctttcaaatg atctaagaca
     8821 gttttaccaa atggttttaa cgcatagtca tgtcgattag atgtacgtgt aaagtttcct
     8881 ggttcaccaa catatggacg tgcgataata cgaccaatta aatatttagg gtcttttgtc
     8941 aactcacgaa ccttttcaca aatatcatat aactcttcta atgggataat gtcttcatgt
     9001 gcagcaattt gcaatactgg gtctgcactt gtataaacaa ttaagtcacc agttttcatt
     9061 tggtgctcgc cccactcatc gataatttgc gtacccgatg ccggtttgtt agcaacaact
     9121 ttacgacctg tcatttcttc aatttgttga attaactctt cagggaatcc attagggtat
     9181 actttaaaag gctgcataat atttaatccc ataatttccc agtgaccagt cattgtatct
     9241 ttaccaactg aagcttcact caatttagta tagtatgctt ctggttgttc aactgcattt
     9301 actactggta atttatcgat gtttcctaga cctaactttt caaggtttgg taaagtttga
     9361 tcgaagcctt ctaaggtatg tcttaaagta tgtgaacctt catctttaaa atcagctgcg
     9421 tctggcgctt caccaatacc tactgaatcc attacgatta aatgtacacg attaaatggt
     9481 cttgtcatag ctatcactcc caaattttat atatattagt aatctgaatc tgcttctaaa
     9541 ccttgcataa tttgaacgcc tgcgctcgca ccaatacgtg tcgcacccgc ttcaaccatt
     9601 ttattgaaat cttctaaatt acgtacgcca cctgatgctt ttacttctac atctgcacct
     9661 actgtatctt tcattaattt aacgtcttct gcagtcgcac cgccacctgc aaaacctgtt
     9721 gaagttttaa cgaagtccgc accagccgct tttgttaatt cactcgcttt tacgatttcg
     9781 tcatggtcca acaataccgt ctcaataatc acttttactg tgtgaccttc cgcagcttta
     9841 accactgctt caatgtcttg ttgtacatca tcaaaacgcc catcttttaa tgcgccgatg
     9901 ttgatgacca tgtcaatttc atctgcacca ttttgaattg catcttctgt ttcaaatact
     9961 ttcgttgcag ttgtcgacgc acctaatggg aatcctatta ccgtacaaac tagcacctct
    10021 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
    10081 aaattgtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
    10141 ttcaataaag tgtgatctat atatttctca aatttcatac ttactactcc tcgtgttata
    10201 taatctcttt atttaatttt actataaata cgaatatatc tcgcgaattt atatctattt
    10261 atttttcaga ttgtgtccaa cgatttaaga tgacgaataa aactaatccg acaatactca
    10321 ttaaacctaa aataattaaa ataataccga aatgtgaact taatgcatca ttgcctggga
    10381 aatttaatgc tttaaaatcg attaaagccg cagcaatcgc aatacctaca gataccgcca
    10441 cattaataat taaattataa aaaccaatag ccacacctgt catattaaga tctattgttt
    10501 taatggcttc gttaagtaaa ggtgcataca ttaaagcaaa gctacctgca aagaatatca
    10561 tagaaatgac gaagattgaa atgtgattac ctactgcaaa tgcaggtaaa atcaagctca
    10621 gtgcgattaa aataattgct gtgataatcg cttgttttga attaagatat tcgccgattt
    10681 taccacttag tgcaccaacg atgactgcta caatataacc cggtactaat agcagtgatg
    10741 ttgtgtctag ctgaagatga taaatttgtt ccattatgaa tgggaacgta aaaatataac
    10801 ccaattggat ggcatacatt acaaatacta taaataaaaa tgaagcataa cgtttatttt
    10861 ggaaaaatga tttgtttact aatggacgtt gcgcattttt aatatatagc gcaaaaacga
    10921 taatcgcaat taaggcacca atcatatata accaattaaa gttcgtaata aacagcataa
    10981 ctgttgtagc gatagttgcc actagaatta agccaacaaa atctaaatga gctttatttg
    11041 tattattttc ttttggtaat aatttaaata ggaatggcaa cgtaaatact acgattaatg
    11101 caattaaaaa catatttgtc cagtgtaaat acgtagaaat aaatccacct gataatgtac
    11161 cgataactaa tgacaaggaa taactgctcg tacttaagcc aaggtaagtc ttctggtcct
    11221 ctttagaaag atactttgca acatatatca catataatgt ctctgcagca gctaaacctg
    11281 cagtttgaat aatacgtcca actaaaagta atgcgaattg atgttggaaa atgtaaccaa
    11341 taattgatcc gataatgatt aatatcacgc cataaataaa tagtgtgcgt atactaatgg
    11401 catcagataa tgaagcgtat actacagcgc caataccaat tactaaaccg gctaatgtcg
    11461 cttgccaact tactgtcgtt actgaaatgt ttaagtcatc agcaatggct acagaaatca
    11521 atttgaatga gttgtctata accaaactga atacaaataa gaataataaa ataggtactg
    11581 ctttctttat tttagaatat tcaacattca taatcatcta caccaatgac agtgcaattt
    11641 caatcatatc tgtaaatgca cgttcccttt cctcaggtgt tgttgacgtt tcatgaatta
    11701 aatgatcgct cactgtgaac acacctaatg cttcgacacc agcgtaaatt gcattcatgt
    11761 ataatgcagc tgattccatt tctacaccta aaatacccat acgcatccaa cgttcactcg
    11821 ctgttgtatc cgcgttatag aaaatatcac ttgataacac attacctaca tgatgacgta
    11881 cacctttgtc acgtgctgtt tcaacagctt tttctaataa ctgataagaa gcaattggcg
    11941 caaaatgacc tggtaattga tattgttgaa cgtaatttga atcagtagag gcaccttgtg
    12001 caacaatcac atcatataaa tcaatgtttt cttgcatcgc gccacaagaa ccaacgcgaa
    12061 ttaatttttt acaaccaaat gtatgaatta attcataaga gtaaatgcca atagatggca
    12121 tacccattcc tgaacccatg acagaaactt ttttaccttt atatgttccg gtaaaaccaa
    12181 acatatttcg cactgtattg aactgttcca catcgtccaa atatgtttct gcaatgaact
    12241 tagctcttaa cggatctcct ggcaatagaa ccgtttctgc aatttcgacg tcattcattg
    12301 gtttaatgtg tggtgttgat ttcataaaat attcctccca atatttgtat agtaatttgt
    12361 ctatttgtct aaccatgtat ataataatat tttttaaaag cgctttcaag cattacattt
    12421 tgacaatatt aagaacattt tacagttatc tctactgatc ttgcataaaa ccaatgttca
    12481 agttgtttct atgtttaatt tttataattt ttccaattcg agtaaaaatt attgtatgat
    12541 gagaatgatt aagtaacttt atggaggttg tctacgtgac gcaaacacaa ccaaaatttt
    12601 taacgattta taatacgtta tataaagaaa ttcaaatagg aaaatatccg agtggccatg
    12661 cattacctac tgaaaaggaa ctttgtgcgc gctttgatgt cagtcgtatg actttgcgcc
    12721 aagctataaa attgttagcc gaagatggca tcgtagagag tacaagaggg aagggacact
    12781 ttgttattcc acaaacaaat gcccaacacg cttcaagtat ggatcaattt aagcatccac
    12841 ttgatcaaat attattagat cgtgtatcta tgtcatcaat taattatcgt gttgatttag
    12901 aaagtgaata tacaaatcat ttgtttgcaa accatccttc tgccgtcatt gcaatggaac
    12961 gctattatca aaaaaaggac aaccattcta agcaagctga tgcattttgt tttaccttta
    13021 taccgttaaa tgtcattgac acatttaaag tgaatacaca aaacgaagac gacatgaaga
    13081 cgttcgttga gcaaacgatt tatagcaatg catatcaatc agatttaaaa atgtctatta
    13141 ccaaagcgcc ccacttcaaa aatcatagtc acgtgtttga tggtgataca cattgttggc
    13201 tgattattga aacattgtac gcacaaacgc cctatccgat tatgattaat aagtggtata
    13261 ttccacagga aatatctgaa ttaacactga cacgtataag acaatcggat tattaaaatt
    13321 attgaaaaaa aatcatgtga tgactgttta gctattgttt aatcgacaat aactttcagg
    13381 taatcacatg atttttctac tttaaaacgt atgatgttta tttatttgtt gaacgttgtc
    13441 taaatgctaa gaataaccca gatactaata gtagtgcggc aatacttgca gtaagaatac
    13501 ttgatgtcat actttcacca gcagctggta aaactttttt gttactttgg ttcatttgat
    13561 ggtttgatga ttgcatatga ccttgttgca tgtgtggctg ttggttcatg tgtgtgttct
    13621 gattcatatg acctgtttta ttttgcacca tttcactacc accatgatta acaccatttg
    13681 catctacatt cgtttgataa aaattgccat cttttgttac aggtaacgca tggccattca
    13741 tatctctaac agcatgtggt ttaaataccc ctacattagt tgtaattatc acattgtatt
    13801 tatcagcaat attttgcgtc gcatcataca atttcatatt atagtcttgc atattactgc
    13861 cagcgcttaa ttggggtaaa gctttctcta attcagtata ttgtgcttgc gtcattcctt
    13921 gtggtactac tccgccataa gggcgatgat tttgtacatt ttggctttgt ttattcgaca
    13981 cattcattga agttgtgtca gcatgcgcat tttgaaaagg catacttgag aatactaaag
    14041 tgcttgttac aattaaagaa cctactgttg ctaatttttt cattgtcttt tccccttata
    14101 ttacaatttg attacattta tattatcata tgattacaaa agaaatgcaa caaaattttt
    14161 gaatcattac aattttttat aaaaatttca ctttagattc acaataatta cttattttgt
    14221 caatttattt aatgtcaata tgttgattaa tcaatagtgt tgtctgatgt atatagcatt
    14281 taagtcatcg ttatagtcaa caataataag gtatttcgag ttgaaattta tcttattatt
    14341 ttgccacttt tacgcgctat cccattacac aaaaacgaat aagtaaagat attaagtaca
    14401 aaaaaggtga aacacctaat agatgctcca cctcaattat attaagttat attattttgc
    14461 tgcttggtat aattcatcaa cttttttcca gttaacaata ttccaaaatg cagtcatata
    14521 gtctggacgt ttattttgat atttcagata gtaggcatgc tcccaaacat caaatagtaa
    14581 gattggtgtt ttgccttctg ttaatggatt atcttggttt ggcgttgtca caatttctaa
    14641 ttttccatca ttaacaacta accaagtcca acctgatcca aataatgttg ttgctttatt
    14701 tgcaaattca tttttaaatt catctaaagt gccccactgc gctttaatgt cgtctattac
    14761 gccacctttt tcttcagaat taggtgatag tatttcccag aataatgaat ggttaaaatg
    14821 accaccgcca ttattacgga ctgacatcct catcgcttcc ggtaccttgt ctaagttagc
    14881 aatcatatct gctagtgatt gatgctctaa ctctgttcct tcaactgttg cgtttaattt
    14941 cgtcacgtac gtattgtgat gtttgtcgtg atgaaactcc attgttcttt gatctatata
    15001 tggttccaat gcatcatatg catatggtaa atttggtaat ttaaatgcca taagtatatt
    15061 cctcctttta tgaatatact tttataataa ttaattttgg gtgtgttttg caataattat
    15121 tatttatttg ttatatccaa ttatataaaa tttaaatatt cgaaattgat ataatattta
    15181 atattgaatg caaaaaggca tttaactgct tttgtttccc gcttttaaag agaattaaaa
    15241 aagacaactt ccatttttca ataagaaatt gccttttctt cttattctta aatttctaaa
    15301 gtatagcgtt accgatactt aaattgtttt atgtcgaaga tgcctcaata tttgccatac
    15361 gtattgattt ttcatagtca tcattaataa aagataaact actataccaa tcgcaattaa
    15421 tagcagcgta gcgtacattg taggaggcaa ataatgattg accacaccaa gcacaataaa
    15481 catcataacg gcagcaatga cacattgaat cgtacttaca atattcaact tcacattgat
    15541 tttagtaata tcaataaatc gccaaatgag caagaaaaac tctgtaatta aacgcgcaat
    15601 agcagcaccg taaattccat aaaaatatat caaaacaaga cataatacta aattcattac
    15661 cgcaccaata gtgattgacg cattatataa tcttatttta ttcactatta ataaatattg
    15721 cctgcttatc aacatattta aaggaatgat taatacaagt atcgctaaaa tggtcatcaa
    15781 tgggacagtt gatgcgaatt cctcaccaaa gaaccataaa taaaatgatg gcataattgc
    15841 aatcaaacca aagaccatag gtattgttaa tatcaattga atattcatat tattagctaa
    15901 cgttttagtt aaactatgtg attgctggat acacatttta gtaatacgcg gaatcattac
    15961 aagatcaaat gtattaatca ttataattgc gaccgttaaa atattaaatg cgttagaaaa
    16021 gataccaact tgttggtatg tgcctactaa accaagaaca acgcaagaaa tactagtgta
    16081 taagttgagt tgaccatttg gtaataaata tgctaatgac gaacgaaaca attgccagac
    16141 gtgtatccaa ttaaccgaaa caaagctaat gtatcgtttt aaatagataa acaaaggtaa
    16201 ttggtttaat actgtcacaa tagcaatagt aaatacatac aatgataaat ctgattgatc
    16261 tttcacaaag ataacaacga cacttaatac aataacagac gcaacaatat tactaaggct
    16321 aggtatttta aacttttcag ttccagcata aaaccatgaa atatcgagtt ctgcacctat
    16381 aatatagatt ccttgtagta ggaaaataag atagtaatca tctataaata tagtaattac
    16441 gaccatatac atcgcaaaaa ctgttaacgc taaaaataat ttactgacaa agatatccca
    16501 aaactgctga gacaattgcc gtttgtcgtt aacggacttc gcgataactc tattaaaata
    16561 taactgaacg ccaacacttg caatcatcaa aaagtattgc acgatattga aagaaaatga
    16621 aacaataccc acaccactgg gaccaaatgc acgtgaaata atgggtattg taatcagcgg
    16681 tgtcatcgtt ctaatcaatt ggtatagccc ttgataaata atattttctt ttagtgaatc
    16741 actcttcatt acgatccctt tccatctttt catattgaac aataaagacg ataattccta
    16801 aaataaaagc gacatatctt gagttattaa acgatactgt taaaaagtaa atcagcatcg
    16861 tcaacattac aacaattgct gttacatttt tcccagaaat attgaatcta aataattcaa
    16921 ataataaata aatcataaat atgataaata acgcaccgaa taaaatgccc cattccgcaa
    16981 aaatttgcaa atatgtatta tgggcaacaa gtaaaatacc attaatttgc gagccaatat
    17041 gtacataatc cactaagcca acaccaaaac ctaatgtata tttaattact gaaatggcat
    17101 ttatccatac aacacttcgc tcagatccac tatcatttaa tgatgcaaac ccctcttcaa
    17161 aaatagacgc cattcgatct aatgacggta aggcatcaag gtcgcttaat tggaataaat
    17221 agtagtttat attataaaag gtaaaacaaa gtagaattaa cataatcact aacatactca
    17281 caacacttac tgcatttcta ctaaataact ttttaataaa gaaataaatg gctaagacga
    17341 ttaatatgat aaacgcagtc ttagaccccg ttgtagttaa agaccatagc aaaataccac
    17401 atgcaaggac cttgaatatg taattatgaa tatacttgta agcaagtacc aatgtaataa
    17461 tctgtgtcat cgcgaaatag ttagggtcat tcattaatcc ttttgaacgt atttcatcaa
    17521 agtataataa tttcattagt aaaggggact tgttcaaacc agctataata caaaatagac
    17581 ctatagtcac actgcttata atataagagg taacgatcac tttttt
//
LOCUS       DATTQF010000042        17451 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.042, whole genome shotgun sequence.
ACCESSION   DATTQF010000042 DATTQF010000000
VERSION     DATTQF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.042"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            134..904
                     /locus_tag="VB241_001226"
     CDS             134..904
                     /locus_tag="VB241_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP1244529.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            911..1693
                     /locus_tag="VB241_001227"
     CDS             911..1693
                     /locus_tag="VB241_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP1244530.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            1905..3191
                     /gene="rseP"
                     /locus_tag="VB241_001228"
     CDS             1905..3191
                     /gene="rseP"
                     /locus_tag="VB241_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP1244531.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGI
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFKSFLIGSTYIFSAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            3211..4914
                     /locus_tag="VB241_001229"
     CDS             3211..4914
                     /locus_tag="VB241_001229"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP1244532.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKAGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPIEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            5178..9488
                     /locus_tag="VB241_001230"
     CDS             5178..9488
                     /locus_tag="VB241_001230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP1244533.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKSKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEYKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGETLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            9778..10245
                     /gene="rimP"
                     /locus_tag="VB241_001231"
     CDS             9778..10245
                     /gene="rimP"
                     /locus_tag="VB241_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP1244534.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            10266..11441
                     /gene="nusA"
                     /locus_tag="VB241_001232"
     CDS             10266..11441
                     /gene="nusA"
                     /locus_tag="VB241_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP1244535.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVTDVSVETNVEKESE"
     gene            11462..11746
                     /locus_tag="VB241_001233"
     CDS             11462..11746
                     /locus_tag="VB241_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP1244536.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11743..12060
                     /locus_tag="VB241_001234"
     CDS             11743..12060
                     /locus_tag="VB241_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HEP1244537.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SNNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            12065..14182
                     /gene="infB"
                     /locus_tag="VB241_001235"
     CDS             12065..14182
                     /gene="infB"
                     /locus_tag="VB241_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP1244538.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQSQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            14569..14919
                     /gene="rbfA"
                     /locus_tag="VB241_001236"
     CDS             14569..14919
                     /gene="rbfA"
                     /locus_tag="VB241_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP1244539.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            15089..16006
                     /gene="truB"
                     /locus_tag="VB241_001237"
     CDS             15089..16006
                     /gene="truB"
                     /locus_tag="VB241_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP1244540.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEMKGVHSANFNNDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            16021..16992
                     /gene="ribF"
                     /locus_tag="VB241_001238"
     CDS             16021..16992
                     /gene="ribF"
                     /locus_tag="VB241_001238"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEP1244541.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVNKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            17107..17376
                     /gene="rpsO"
                     /locus_tag="VB241_001239"
     CDS             17107..17376
                     /gene="rpsO"
                     /locus_tag="VB241_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEP1244542.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT         6332 a         2358 c         3433 g         5328 t
ORIGIN      
        1 ctgtaccgag tcagactttg gtacagtttt tttatttgct tattcaatgc attaaatgag
       61 tatgataaaa tgataatgat tgtttagtaa cttatactat atgacagaga tgatcaggct
      121 cggaggaaag accatgttta aaaagctaat aaataaaaag aacactataa ataattataa
      181 tgaagaatta gactcgtcta atatacctga acatatcgct attattatgg atggtaatgg
      241 gcgatgggct aagaagcgaa aaatgcctag aattaaaggt cattacgaag gtatgcaaac
      301 aataaaaaaa attactaggg tagctagtga tattggtgtt aagtacttaa ctttatatgc
      361 cttttccact gaaaattggt caagacctga aagtgaagta aattatatta tgaatttgcc
      421 tgtcaatttc ttaaagacat ttttaccgga actaattgaa aaaaatgtca aagttgaaac
      481 aattggattt actgataagt tgccaaaatc aacgatagaa gcaataaata atgctaaaga
      541 aaagacagct aataataccg gcttaaaatt aatatttgca attaattatg gtggcagagc
      601 agaacttgtt catagtatta aaaatatgtt tgacgagctt catcaacaag gtttgaatag
      661 tgatatcata gatgaaacat atataaacaa tcatttaatg acaaaagact atcctgatcc
      721 agagttgtta attcgtactt caggagaaca aagaataagt aatttcttga tttggcaagt
      781 ttcgtatagt gaatttatct ttaatcaaaa attatggcct gactttgacg aagatgaatt
      841 aattaaatgt attaaaattt atcagtcacg tcaaagacgc tttggcggat tgagtgagga
      901 gtagtatagt atgaaagtta gaacgctgac agctattatt gccttaatcg tattcttgcc
      961 tatcttgtta aaaggcggcc ttgtgttaat gatatttgct aatatattag cattgattgc
     1021 attaaaagaa ttgttgaata tgaatatgat taaatttgtt tcagttcctg gtttaattag
     1081 tgcagttggt cttatcatca ttatgttgcc acaacatgca gggccatggg tacaagtaat
     1141 tcaattaaaa agtttaattg caatgagctt tattgtatta agttatactg tcttatctaa
     1201 aaacagattt agttttatgg atgctgcatt ttgcttaatg tctgtggctt atgtaggcat
     1261 tggttttatg ttcttttatg aaacgagatc agaaggatta cattacatat tatatgcctt
     1321 tttaattgtt tggcttacag atacaggggc ttacttattt ggtaaaatga tgggtaaaca
     1381 taagctttgg ccagtaataa gtccgaataa aacaatcgaa ggattcatag gtggcttgtt
     1441 ctgtagtttg atagtgcctc ttgcaatgtt atattttgta gatttcaata tgaatgtatg
     1501 gatattactt ggagtgacat tgattttaag tttatttggt caattaggtg atttagtgga
     1561 atcaggattt aagcgtcatt tcggcgttaa agactcaggt cgaatactac ctggacacgg
     1621 tggtatttta gaccgatttg acagttttat gtttgtgtta ccattattaa atattttatt
     1681 aatacaatct taatgctgag aacaaatcaa taaacgtaaa gaggagttgc tgagataatt
     1741 taatgaatct cagaactcct tttgaaaatt atacgcaata ttaactttga aaattagacg
     1801 ttatattttg tgatttgtca gtatcatatt ataatgactt atgttacgta tacagcaatc
     1861 atttttaaaa taaaagaaat ttataaacaa tcgaggtgta gcgagtgagc tatttagtta
     1921 caataattgc atttattatt gtttttggtg tactagtaac tgttcatgaa tatggccata
     1981 tgttttttgc gaaaagagca ggcattatgt gtccagaatt tgcgatcggt atggggccga
     2041 aaatttttag ttttagaaaa aatgaaacac tttacactat taggttattg cctgttggtg
     2101 gatatgttcg tatggcagga gatggcttag aagagccacc agtcgagcct ggtatgaacg
     2161 ttaaaattaa actgaatgaa gaaaatgaaa taacacatat catattagat gatcatcata
     2221 agtttcaaca aattgaagcg atcgaagtta aaaaatgtga ttttaaagat gacttattca
     2281 tagaaggtat cactgcttat gataatgaaa gacatcattt taaaattgct agaaagtctt
     2341 tctttgttga aaatggtagc ttagttcaaa ttgctccgag agacagacaa tttgcacata
     2401 aaaagccatg gccgaaattt ttaacattat ttgcgggacc gttatttaac tttatattag
     2461 ctttagttct atttattggt cttgcatatt atcaaggtac acctacttct actgtagaac
     2521 aagtcgcaga taagtatcca gctcaacaag caggcataca aaaaggcgat aaaattgtcc
     2581 aaattggcaa atataaaata tctgaatttg atgatgttga taaggcgtta gataaagtta
     2641 aagataataa gacgactgtt aaatttgaac gtgatggtaa aacaaagtca gttgaattaa
     2701 cacctaaaaa gaccgaaaga aaactgacta aagtaagttc agagacgaag tatgttctcg
     2761 gattccaacc agcgagtgaa catacactct tcaaaccaat tgtatatgga tttaaaagct
     2821 ttttaatcgg tagtacttat attttttcag ctgtagtagg tatgttggct agtatattta
     2881 cgggcggatt ctcgtttgat atgttaaatg gtccggttgg tatttatcat aacgtcgact
     2941 cagttgttaa agcgggtatc attagcttaa ttggatacac tgcgttatta agtgtaaact
     3001 taggtattat gaatttaatt cctattcctg cactagacgg tggtcgtatt ttatttgtta
     3061 tatatgaagc gattttcaga aaaccagtta ataaaaaagc ggaaacaacg attatcgcta
     3121 ttggtgccat tttcatggtc gttattatga tattagtaac gtggaatgat attcgacgat
     3181 atttcttata atttaggagg ataaataatt atgaagcaat ccaaagtttt tataccaacg
     3241 atgcgtgacg tgccatcaga agcagaagca caaagtcatc gtttattatt gaaagcgggt
     3301 ttgataaaac aaagtacaag tgggatttat agttatttac cgctagcaac acgtgtgtta
     3361 aataatatta ctgcaattgt gcgacaagaa atggaacgta tcgattctgt tgaaatttta
     3421 atgccagcgt tacaacaagc tgaattatgg gaagaatcag gacgttgggg tgcatatggc
     3481 ccagaattaa tgcgtttaca agatagacat ggaagacaat ttgcattagg tccaacacat
     3541 gaagaattag ttacatcaat agtaagaaat gaattgaaat catacaaaca attaccgatg
     3601 acattattcc aaattcaatc taaattccgt gatgaaaaga gaccacgttt tggtttactt
     3661 cgtggtcgtg aatttattat gaaagatgcg tattcattcc atgctgacga ggcatcatta
     3721 gatcaaacgt atcaagatat gtatcaagcg tatagccgta tttttgagag agttggcatt
     3781 aacgcaagac cagtagttgc agattcaggt gctattggcg gtagccatac acatgaattt
     3841 atggcattaa gtgctatcgg tgaggataca atcgtttaca gtaaagaaag tgactatgct
     3901 gctaacatcg aaaaagcaga agtcgtttac gaaccaaatc ataagcatac tactgtgcaa
     3961 cctatagaaa aaattgaaac accaaatgtt aagactgcgc aagaattggc agacttctta
     4021 ggtagaccag tagatgaaat cgttaaaacg atgattttca aagttgatgg cgaatatatt
     4081 atggttttag tgcgtggaca tcatgaaatt aatgacatta aattaaaatc ttatttcggc
     4141 acagataata ttgaattagc aacacaagac gaaattgtta atttagttgg tgcaaatcct
     4201 ggttcactag gtcctgtaat tgataaagag atcaaaattt atgcagataa ttttgtgcaa
     4261 gatttaaata atttagttgt cggggctaac gaagatggtt atcacttaat taatgtaaat
     4321 gtaggtagag acttcaacgt tgatgaatat ggcgatttcc gttttatttt agaaggcgaa
     4381 aagttaagtg atggttcagg cgttgcacat tttgctgaag gaattgaagt tggtcaagta
     4441 ttcaaattgg gtactaagta ttcagaatca atgaatgcta cattcttaga taatcaagga
     4501 aaagcccaac cgttaattat gggttgttac ggtattggaa tttctagaac gctaagtgcg
     4561 attgttgaac aaaatcacga tgataatggt attgtttggc ctaaatcagt tactccattt
     4621 gatttacatt taatttctat taatcctaag aaagatgatc aacgagaact agcggatgca
     4681 ctatatgctg aatttaatac taaatttgat gtgttgtacg atgatcgtca ggaacgtgct
     4741 ggtgtcaaat ttaatgatgc cgatttaatt ggtttaccac tgcgaattgt tgttggtaaa
     4801 cgtgcatcgg aaggtattgt agaagttaaa gaacgtttaa caggtgatag cgaagaagtc
     4861 cacattgatg acttaatgac tgtcattaca aataaatatg ataatttaaa ataattaaga
     4921 tcgaatgaat tataagagta ggaaaaagct gaaagaaatc agatgcttat gtcctgctct
     4981 tattattttt gatataatga ttattcgatg aaaaatgact gaagacatag tataattaaa
     5041 gataaatttg ttttaacaat attatgatta gccaaatata aagcatttaa ttttctatca
     5101 ttactatgct cacataatct aaatattgtt cgaacacgta aaagtaattt ctatttaagg
     5161 tggtaattgt cttggcaatg acagagcaac aaaaatttaa agtgcttgct gatcaaatta
     5221 aaatttcaaa tcaattagat gctgaaattt taaattcagg tgaactgaca cgtatagatg
     5281 tttctaacaa aaacagaaca tgggaatttc atattacatt accacaattc ttagctcatg
     5341 aagattattt attatttata aatgcaatag agcaagagtt taaagatatc gccaacgtta
     5401 catgtcgttt tacggtaacg aatggcacga atcaagatga acatgcaatt aaatactttg
     5461 ggcactgtat tgaccaaaca gctttatctc caaaagttaa aggtcaattg aaacagaaaa
     5521 agcttattat gtctggaaaa gtattaaaag taatggtatc aaatgacatt gaacgtaatc
     5581 attttgataa ggcatgcaat ggaagtctta tcaaagcgtt tagaaattgt ggttttgata
     5641 tcgataaaat catattcgaa acaaatgata atgatcaaga acaaaactta gcttctttag
     5701 aagcacatat tcaagaagaa gacgaacaaa gtgcacgatt ggcaacagag aaacttgaaa
     5761 aaatgaaagc tgaaaaatcg aaacaacaag ataacaacga aagtgctgtc gataagtgtc
     5821 aaattggtaa gccgattcaa attgaaaata ttaaaccaat tgaatctatt attgaggaag
     5881 agtataaagt tgcaatagag ggtgtcattt ttgatattaa cttaaaagaa cttaaaagtg
     5941 gtcgtcatat cgtagaaatt aaagtgactg actatacgga ctctttagtt ttaaaaatgt
     6001 ttactcgtaa aaacaaagat gatttagaac attttaaagc gctaagtgtt ggtaaatggg
     6061 ttagggctca aggtcgtatt gaagaagata catttattag agatttagtt atgatgatgt
     6121 ctgatattga agagattaaa aaagcgacaa aaaaagataa ggctgaagaa aaacgtgtag
     6181 aattccactt acatactgca atgagtcaaa tggatggtat accaaatatt ggtgcgtatg
     6241 ttaaacaggc agcagactgg ggacatccag ccattgcggt tacagaccat aatgttgtgc
     6301 aagcatttcc agatgctcac gcagcagcgg aaaaacatgg tattaaaatg atatatggta
     6361 tggaaggtat gttagttgat gatggtgttc cgattgcata caaaccacaa gatgtcgtat
     6421 taaaagatgc tacttatgtt gtattcgacg tcgagacaac tggtttatca aatcagtatg
     6481 ataaaatcat cgagcttgca gctgtgaaag ttcataacgg cgaaatcatc gataagtttg
     6541 aaaggtttag taatccgcat gaacgattat cggaaacgat tatcaatttg acgcatatta
     6601 ctgatgatat gttagtagat gcccctgaga ttgaagaagt acttacagaa tttaaagaat
     6661 gggttggcga tgcgatattc gtagcacata atgcttcgtt tgatatgggc ttcatcgaca
     6721 cgggatatga acgtcttgga tttggaccat ctacgaatgg tgttatcgat acattagaat
     6781 tatctcgtac gattaatact gaatatggta aacatggttt aaatttctta gctaaaaaat
     6841 acggtgtaga attaacgcaa catcaccgtg ccatttatga tacagaggca acagcttaca
     6901 ttttcataaa aatggttcaa caaatgaaag aattaggcgt attaaatcat aacgaaatta
     6961 acaaaaaact cagtaatgaa gatgcatata aacgtgcaag acctagtcat gtcacattaa
     7021 ttgtacaaaa tcaacaaggt cttaaaaatc tatttaaaat tgtaagtgca tcattggtga
     7081 agtatttcta ccgtacgcct cgaattccac gttcattgtt agatgaatat cgtgagggat
     7141 tattggtagg tacagcgtgt gatgaaggtg aattatttac ggcagttatg cagaaggacc
     7201 agagccaagt tgaaaaaatt gccaaatatt atgattttat tgaaattcaa ccaccggcac
     7261 tttatcaaga tttaattgat agagagctta ttagagatac tgaaacatta catgaaattt
     7321 atcaacgttt aattcatgca ggtgacacag cgggtattcc tgttattgcg acaggaaatg
     7381 cacactattt gtttgaacat gatggcatcg cacgtaaaat tttaatagca tcacaacccg
     7441 gcaatccact taatcgctca actttaccgg aagcacattt tagaactaca gatgaaatgt
     7501 taaacgagtt tcatttttta ggtgaagaaa aagcgcatga aattgttgtg aaaaatacaa
     7561 acgaattagc agatcgaatt gaacgtgttg ttcctattaa agatgaatta tacacaccgc
     7621 gtatggaagg tgctaacgaa gaaattagag aactaagtta tgcaaatgcg cgtaaactgt
     7681 atggtgaaga tctgcctcaa atcgtaattg atcgattaga aaaagaatta aaaagtatta
     7741 tcggtaatgg atttgcggta atttacttaa tttcgcaacg tttagttaaa aaatcattag
     7801 atgatggata cttagttggt tcccgtggtt cagtaggttc tagttttgtg gcgacaatga
     7861 ctgagattac tgaagtaaac ccgttaccgc cacactatat atgtccgaac tgtaaaacga
     7921 gtgaattttt caatgatggt tcagtaggat caggattcga tttacctgat aagacgtgtg
     7981 aaacttgtgg agcgccactt attaaagaag gacaagatat tccgtttgaa acatttttag
     8041 gatttaaagg agataaagtt cctgatatcg acttaaactt tagtggtgaa tatcaaccaa
     8101 atgcccataa ctacacaaaa gtattatttg gtgaggataa agtattccgt gcaggtacaa
     8161 ttggtactgt tgctgaaaag actgcttttg gttatgttaa aggctatttg aatgatcaag
     8221 gcatccacaa aagaggtgct gaaatagatc gactcgttaa aggatgtaca ggtgttaaac
     8281 gtacaactgg acagcatcca gggggtatta ttgtagtacc tgattacatg gatatttatg
     8341 attttacgcc gatacaatat cctgccgatg atcaaaattc agcatggatg acgacacatt
     8401 ttgatttcca ttctattcat gataatgtat taaaacttga tatacttgga cacgatgatc
     8461 caacaatgat tcgtatgctt caagatttat cagggattga tccaaaaaca atacctgtag
     8521 atgataaaga agttatgcag atttttagta cacctgaaag tttgggtgtt actgaagatg
     8581 aaattttatg taaaacaggt acatttgggg taccagaatt cggtacagga ttcgtgcgtc
     8641 aaatgttaga agatacaaag ccaacaacat tttcagaatt agttcaaatc tcaggattat
     8701 ctcatgggac agatgtgtgg ttaggtaatg ctcaagaatt aattaaaaca ggtatatgtg
     8761 atttatcaag tgtaattggt tgtcgtgacg atatcatggt ttatttaatg tatgctggtc
     8821 tagaaccatc aatggctttt aaaataatgg agtcagtacg taaaggtaaa ggtttaactg
     8881 aagaaatgat tgaaacgatg aaagaaaatg aagtgccgga ttggtattta gattcatgtc
     8941 ttaaaattaa gtacatgttc cctaaagccc atgcagcagc atacgtttta atggcagtac
     9001 gtatcgcata tttcaaagta catcatccac tttattacta tgcatcttac tttacaattc
     9061 gtgcgtcaga ttttgattta atcacgatga ttaaagataa aacaagcata cgaaatactg
     9121 taaaagacat gtattctcgc tatatggatc taggtaaaaa agaaaaagac gtattaactg
     9181 tcttggaaat tatgaatgaa atggcgcatc ggggttatcg aatgcaaccg attagtttag
     9241 aaaagagtca agcgttcgaa tttatcattg aaggcgaaac acttattccg ccgttcatat
     9301 cagtgcctgg gcttggcgaa aacgttgcga aacgaattgt tgaagctcgt gacgatggac
     9361 catttttatc aaaagaagat ttaaacaaaa aagctggatt atctcagaaa attattgagt
     9421 atttagatga gttaggctca ttaccgaatt taccagataa agctcaactt tcgatattcg
     9481 atatgtaaaa tgaaataatc aaggtattta tttaattcgt atggcgtagt taaagaaata
     9541 caaaattgtt gctggacaca aagttatacc tgtatttctt ttcaacgtct tacgagtcta
     9601 ttcgaatgta atgatgaaat aaatgaacaa acttttacaa gaatctctga ttaatagtga
     9661 agtcatttgt ttcaagcata aacttatgct ataattaagt tgcttaaaaa ttagtgaact
     9721 caggcagaag agtgggagat tcccgctctt ttctatttgc caaaaaggga ggcctgtatg
     9781 agtaaaatta cagaacaagt agaagtgatt gttaaaccaa ttatggaaga cttgaatttt
     9841 gaacttgtag acgttgaata tgtcaaagag ggtagagatc attttcttag aatctctatt
     9901 gataaagaag gtggcgtaga tttaaatgat tgtacgctag cttctgaaaa aataagtgaa
     9961 gctatggatg caaatgatcc tattcctgaa atgtattatt tagacgtagc gtcacctggt
    10021 gcagaacgtc caattaaaaa agaacaagat ttccaaaatg caataactaa acctgtattt
    10081 gtttctttat atgtaccaat tgaaggtgaa aaggaatggt taggcatttt acaagaagtc
    10141 aataatgaaa caattgtagt acaagttaaa atcaaagcaa gaacgaaaga tatagagata
    10201 ccgagagaca aaatagcaaa agcacgtcac gcagttatga tttaacgtga tgaggaggaa
    10261 aaaacgtgtc aagtaatgaa ttattattag ctactgagta tttagaaaaa gaaaagaaga
    10321 ttcctagagc agtattaatt gatgctattg aagcagcttt aattactgca tacaaaaaga
    10381 attatgatag tgcaagaaat gtccgtgtgg aattaaatat ggatcaaggt actttcaaag
    10441 ttatcgctcg taaagatgtt gttgaagaag tatttgacga cagagatgaa gtggatttaa
    10501 gtacagcgct tgttaaaaac cctgcatatg aaattggtga tatatacgaa gaagatgtaa
    10561 cacctaaaga ttttggtcgt gtaggtgctc aagcagcgaa acaagcagta atgcaacgtc
    10621 ttcgtgatgc tgaacgtgaa attttatttg aagaatttat agacaaagaa gaagacatac
    10681 ttactggaat tattgaccgt gttgaccatc gttatgtata tgtgaattta ggtcgtattg
    10741 aagctgtttt atctgaagca gaaagaagtc ctaacgaaaa atatattcct aacgaacgta
    10801 tcaaagtata tgttaacaaa gtggaacaaa cgacaaaagg tcctcaaatc tatgtttctc
    10861 gtagccatcc aggtttatta aaacgtttat ttgaacaaga agttccagaa atttacgatg
    10921 gtactgtaat tgttaaatca gtagcacgtg aagctggcga tcgctctaaa attagtgtct
    10981 tctctgaaaa caatgatata gatgctgttg gtgcatgtgt tggtgccaaa ggcgcgcgtg
    11041 ttgaagctgt tgttgaagag ctaggtggcg aaaaaatcga tatcgttcaa tggaatgaag
    11101 atccaaaagt atttgtaaaa aatgctttaa gcccttctca agtgttagaa gttattgttg
    11161 atgaaacaaa tcaatctaca gtagttgttg ttcctgatta tcaattgtca ttagcgattg
    11221 gtaaaagagg acaaaacgca cgtctagctg ctaaattaac cggctggaaa attgatatta
    11281 aatcagaaac agatgcgcgt gaagcgggta tctatccagt agttgaagct gaaaaagtaa
    11341 ctgaagaaga tgttgcttta gaagatgctg acacaacaga atcaaccgaa gaggtaactg
    11401 atgtttcagt tgaaacaaat gtagagaaag aatctgaata ataggttgga gtgaagtatc
    11461 tatgaaaaag aaaaaaattc cgatgcgaaa atgtattctt tcaaatgaaa tgcatcccaa
    11521 aaaagatatg attcgtgttg ttgttaataa agaaggcgaa atctttgcgg atgttactgg
    11581 aaagaaacaa ggccgtggcg cgtatgtttc taaagatgtt gctatggttg aaaaagcaca
    11641 acaaaaagaa attttagaaa aatattttaa agcatctaaa gagcaattgg atccggttta
    11701 caaagaaatt attagattaa tttatagaga agagatccca aaatgagtat agatcaaata
    11761 ttaaactttt taggattagc aatgagagcc ggtaaagtaa aaacaggtga atcagtcatt
    11821 gttaatgaaa ttaaaaaagg aaatttgaag ctcgttattg ttgcaaatga tgcgtctaat
    11881 aatacagcta aattaattac agataaatgt aagagttaca aagttccatt cagaaagttt
    11941 ggaaatcgaa atgaattggg aatagcactt ggaaaaggtg agcgtgttaa tgtagggatt
    12001 actgacccag gctttgctaa aaagttgcta tcaatgatag atgaatatca taaggagtga
    12061 ttatatgagt aaacaaagaa tttacgaata tgcgaaagaa ttaaatctaa agagtaaaga
    12121 gattatagat gagttaaaaa gcatgaatat tgaggtttca aatcatatgc aagctttgga
    12181 agatgaccaa attaaagcat tagataaaaa gttcaaaaaa gaacaaaaga acgacaataa
    12241 acaaagcact caaaataatc accaaaaatc aaacaatcaa aatcaaaata aagggcaaca
    12301 aaaagataac aaaaagaatc aacaacaaaa taataaaggc aacaaaggca ataaaaagaa
    12361 taatagaaat aataagaaaa ataacaagaa taataaacca caaagtcaac cagctgctcc
    12421 aaaagaaata ccatcaaaag tgacatatca agaaggtatt acagtaggcg aatttgcgga
    12481 taaattaaat gttgaatcat cagaaattat caaaaaatta ttcttacttg gtattgttgc
    12541 taatatcaat caatcattaa atcaagaaac aatcgaatta attgccgatg attatggcgt
    12601 tgaggttgaa gaagaagttg tgattaatga agaagattta tcaatctact tcgaagacga
    12661 aaaagatgat ccagaagcaa ttgagagacc agctgttgta acaattatgg gacatgttga
    12721 ccatggtaaa actactttat tagattcaat tcgtcataca aaagttacag caggtgaagc
    12781 aggcggaatc actcaacata ttggtgcata tcaaattgaa aacgatggca aaaaaatcac
    12841 tttcttagat acaccgggac atgctgcatt tacaacgatg cgtgcgcgtg gtgcacaagt
    12901 aacagatatt acaattttag tagtagcagc tgacgatggt gttatgccac aaacaattga
    12961 agcaattaac catgctaaag aagcagaagt accaattatt gttgcagtaa ataaaattga
    13021 taaaccaact tcaaatcctg atcgagttat gcaagaatta actgaatatg gtttaattcc
    13081 tgaagattgg ggcggcgaaa caattttcgt tccactttct gcattaagtg gtgatggtat
    13141 cgacgattta ttagaaatga taggattagt tgcagaagtt caagaactta aagcaaatcc
    13201 taaaaaccgt gctgttggta cagttatcga agctgaatta gataaatcac gaggtccttc
    13261 tgcatcatta ttagtacaaa acggtacatt aaatgttggt gatgcgattg tagtgggtaa
    13321 tacttacggc cgtatccgtg caatggttaa tgacttaggt caaagaatca aaacggctgg
    13381 tccatcaacg cctgttgaaa ttacaggtat taatgatgtg ccacaagctg gggatcgctt
    13441 tgttgtattt agtgatgaaa aacaagctcg tcgtattggt gaatcaagac acgaagctag
    13501 cattgtacaa caacgtcaag aaagtaaaaa tgtttcatta gataacctgt ttgaacaaat
    13561 gaaacaaggt gaaatgaaag acttaaacgt tattattaaa ggtgatgttc aaggttctgt
    13621 tgaagcttta gctgcatcat taatgaaaat tgatgttgaa ggcgtaaatg ttcgtatcat
    13681 tcatacagcg gttggtgcga ttaacgagtc agacgtgaca cttgctaatg cctcaaatgg
    13741 tattatcatt ggtttcaatg ttcgtccaga cagtggtgca aaacgtgctg cagaagctga
    13801 aaatgttgat atgcgtttac acagagttat ttataatgtt atcgaagaaa ttgaatcagc
    13861 gatgaaaggt ttacttgatc cagaatttga agaacaagtt atcggacaag ctgaagttcg
    13921 tcaaacattc aaagtttcta aagttggtac tattgctgga tgttatgtta ctgaaggtaa
    13981 aattacgcga aatgctggtg tacgtattat tcgtgatggt attgttcaat atgaaggcga
    14041 attagataca cttaaacgtt tcaaagatga tgctaaggaa gttgcaaaag gttatgaatg
    14101 tggtattaca attgaaaact acaatgacct taaagaaggc gatgttatcg aagcatttga
    14161 aatggttgaa attaagcgtt aattaaataa attacaagct aaaagtttag ttaagattga
    14221 tatgctccct ataaatatta cactttttaa gtgtttactt tatagggagc atatttgata
    14281 caagcttttg gttttttatt agaatagatt acctattaaa agttacgtta tatagacatg
    14341 atttttgtat aaaattttgt ggtgacctag aatgcttttt aatgacaaaa tataatgtcg
    14401 actattattg gaaaattttc tgttgaaatg cctatcttac ggcaaacttt atttgatttt
    14461 ataggcttaa tttattaaaa taacgtgtga gctaaaataa ttgtttaagc attgttacac
    14521 taaaaaatgc aaataacaat tgaacttaaa gataaagagg tgacaagaat gagcagtatg
    14581 agagcagagc gtgttggtga acaaatgaag aaggaattaa tggatatcat caacaataaa
    14641 gtcaaagatc ctcgagttgg ttttattaca attacagatg ttgttttaac aaatgattta
    14701 tcgcaggcta aagtattttt aactgtatta ggtaacgata aagaagtaga aaatacattt
    14761 aaagcacttg ataaagcaaa aggcttcatt aagtctgaat taggttctag aatgcgatta
    14821 cgtattatgc cggaattaat gtatgaatat gatcaatcaa tcgaatatgg taataaaatt
    14881 gaacgaatga ttcaagattt acacaaacaa gatagataat ttagtgttag gtatctggaa
    14941 aatgtttgat aatttcttaa tatcggtata ttaacattaa acagttaata catagatgtg
    15001 tagaaatagt taacattttc cagttttttt atgaataaat tcagttaata cgctattaaa
    15061 atatattttt aaaaaagaag gtgacaacat gtataacggt atattaccag tatataaaga
    15121 acgtggttta acaagtcatg acgtcgtatt caaattgcgt aaaattttaa agactaaaaa
    15181 aatagggcac actggtacgc ttgatcctga agttgcaggc gtgttaccgg tatgtatagg
    15241 taatgcaacg agagttagtg attatgttat ggatatgggt aaagcttatg aggcaacggt
    15301 atcgataggc agaagtacaa caactgaaga tcaaacgggt gatacattgg aaatgaaggg
    15361 tgtacattca gctaatttca acaatgacga tattgaccga ttgttagaaa gttttaaagg
    15421 tatcattgaa caaattccgc caatgtattc atcagtcaag gtgaatggta aaaaattata
    15481 tgaatatgcg cgtaataatg aaacagttga aagaccaaag cgtaaagtta atattaaaga
    15541 tattgggcgt atatctgaat tagattttaa agaaaatgag tgtcatttta aaatacgtgt
    15601 catctgtggc aaaggtacat atattagaac gttagcgact gatatcggtg tgaaattagg
    15661 ctttccggca catatgtcga aattaacgcg aatcgagtct ggtggatttg tgttgaaaga
    15721 tagccttaca ttagaacaaa taaaagaact tcatgagcag gattcattgc aaaataaatt
    15781 gtttccttta gaatatggat taaagggttt gccaagtatt aaaattaaag attcgcacat
    15841 aaaaaaacgt attttaaatg ggcagaaatt taataaaaat gaatttgata acaaaattaa
    15901 agaccaaatt gtatttattg atgatgattc agaaaaagta ttagcaattt atatggtaca
    15961 ccctacaaaa gaatcagaaa ttaaacctaa aaaagtcttt aattaaagga gatagaattt
    16021 atgaaagtca tagaagtgac acatcctata caatctaaac agtatattac agaggatgtt
    16081 gcaatggcat tcggattttt cgatggcatg cataaaggtc atgacaaagt ctttgatata
    16141 ttaaacgaaa tagctgaggc acgcagttta aaaaaagcgg tgatgacatt tgacccgcat
    16201 ccgtctgtcg tgttgaatcc taaaagaaaa cgaacaacct atttaacacc actttcagat
    16261 aaaatcgaaa aaattagcca acatgatatt gattattgta tagtggttaa tttttcatct
    16321 aggtttgcta atgtgagcgt tgaagatttt gttgaaaatt atataattaa aaataatgta
    16381 aaagaagtca ttgctggttt tgattttact tttggtaaat ttggaaaagg taatatgact
    16441 gttcttcaag aatatgacgc gtttaatacg acaattgtga ataaacaaga aattgaaaat
    16501 gaaaaaattt ctacaacttc tattcgtcaa gatttaatca atggtgagtt gcaaaaagcg
    16561 aatgatgctt taggctatat atattctatt aaaggcactg tagtgcaagg tgaaaaaagg
    16621 ggaagaacta ttggcttccc aacagctaac attcaaccta gtgatgatta tttgttacct
    16681 cgtaaaggtg tttatgccgt tagtattgaa atcggcactg aaaataaatt atatcgaggg
    16741 gtagctaaca taggtgtaaa gccaacattt catgatccta acaaagcaga agttgtcatc
    16801 gaagtgaata tctttgactt tgaggataat atttatggtg aacgagtgac agtgaattgg
    16861 catcatttct tacgtcctga gattaaattt gatggtatcg acccattagt taaacaaatg
    16921 aacgatgata aatcgcgtgc taaatattta ttagcagttg attttggtga tgaagtagct
    16981 tataatattt agagttgcgt atagttatat aaacaatcta taccacacct ttttcttagt
    17041 aggtcgaatc tccaacgcct aactcggatt aaggagtatt caaacatttt aaggaggaaa
    17101 ttgattatgg caatttcaca agaacgtaaa aacgaaatca ttaaagaata ccgtgtacac
    17161 gaaactgata ctggttcacc agaagtacaa atcgctgtac ttactgcaga aatcaacgca
    17221 gtaaacgaac acttacgtac acacaaaaaa gaccaccatt cacgtcgtgg attattaaaa
    17281 atggtaggtc gtcgtagaca tttattaaac tacttacgta gtaaagatat tcaacgttac
    17341 cgtgaattaa ttaaatcact tggtatccgt cgttaatctt aatataacgt ctttgaggtt
    17401 ggggcatatt tatgttccaa ccttaattta tattaaaaag ttttttacaa a
//
LOCUS       DATTQF010000043        17426 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.043, whole genome shotgun sequence.
ACCESSION   DATTQF010000043 DATTQF010000000
VERSION     DATTQF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.043"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            249..1415
                     /gene="aroC"
                     /locus_tag="VB241_001240"
     CDS             249..1415
                     /gene="aroC"
                     /locus_tag="VB241_001240"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP1244543.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISDEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLEQLDIEIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDGIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
     gene            1441..2505
                     /locus_tag="VB241_001241"
     CDS             1441..2505
                     /locus_tag="VB241_001241"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP1244544.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFNDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLKGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2515..3813
                     /gene="aroA"
                     /locus_tag="VB241_001242"
     CDS             2515..3813
                     /gene="aroA"
                     /locus_tag="VB241_001242"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP1244545.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEATIIKELDVSRNHTETMFRHFNIPIEAEGLSITTI
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPIKIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3820..5064
                     /locus_tag="VB241_001243"
     CDS             3820..5064
                     /locus_tag="VB241_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244546.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFIWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQMEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5078..5653
                     /locus_tag="VB241_001244"
     CDS             5078..5653
                     /locus_tag="VB241_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEP1244547.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKHNIKLMNTNEIFDYIANHNGAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            5643..6230
                     /locus_tag="VB241_001245"
     CDS             5643..6230
                     /locus_tag="VB241_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HEP1244548.1"
                     /translation="MTINKLWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     QFKIFVPDSPTAILFLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            6302..6982
                     /locus_tag="VB241_001246"
     CDS             6302..6982
                     /locus_tag="VB241_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEP1244549.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            7318..7635
                     /locus_tag="VB241_001247"
     CDS             7318..7635
                     /locus_tag="VB241_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEP1244550.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     KRK"
     gene            7879..9021
                     /gene="bshA"
                     /locus_tag="VB241_001248"
     CDS             7879..9021
                     /gene="bshA"
                     /locus_tag="VB241_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEP1244551.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQKTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDSDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            9026..10228
                     /locus_tag="VB241_001249"
     CDS             9026..10228
                     /locus_tag="VB241_001249"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP1244552.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTANKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDLLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            10215..11186
                     /locus_tag="VB241_001250"
     CDS             10215..11186
                     /locus_tag="VB241_001250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEP1244553.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     DFDESIRYRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            11210..13903
                     /locus_tag="VB241_001251"
     CDS             11210..13903
                     /locus_tag="VB241_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEP1244554.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAEEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFDAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQASGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            14225..15517
                     /gene="asnS"
                     /locus_tag="VB241_001252"
     CDS             14225..15517
                     /gene="asnS"
                     /locus_tag="VB241_001252"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP1244555.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKMLERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            15846..16532
                     /locus_tag="VB241_001253"
     CDS             15846..16532
                     /locus_tag="VB241_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP1244556.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            16522..17181
                     /gene="nth"
                     /locus_tag="VB241_001254"
     CDS             16522..17181
                     /gene="nth"
                     /locus_tag="VB241_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP1244557.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            17186..>17426
                     /locus_tag="VB241_001255"
     CDS             17186..>17426
                     /locus_tag="VB241_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244558.1"
                     /translation="MTNITKEVFDNLEQKIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDNLNQYPVVPMNFKERYDYMIER"
BASE COUNT         6400 a         2331 c         3212 g         5483 t
ORIGIN      
        1 tttaaatttt caaatggttg tttaaacaat ctaacaatat tagaaattca taatactttt
       61 ctcgttgtga atatattata atgaatctta gttcgtttaa tattaagata attctgacat
      121 ttaaaaagag atataaatca atttcttaat tgagcttgaa aacaaacatt tatgaatgca
      181 caatgaatat gataagatta acaacatatt ataatgttat cgtggaagta tgaaaggagc
      241 gagtgtgtat gagataccta acatcaggag aatcacatgg acctcaatta acagttattg
      301 ttgaaggtgt acctgcaaat ttagaaatta aggttgagga tattaataaa gaaatgttta
      361 agcgtcaagg cggttacgga cgtggacgtc gtatgcaaat tgaaaaagat acagtggaga
      421 ttgtttcggg tgtaagaaat ggttatacat taggtagccc tattacaatg gttgttacta
      481 atgatgattt tacacattgg cgaaaaatta tgggcgctgc gccaataagc gatgaagaac
      541 gagaaaatat gaaacgtaca attacgaagc caagaccggg acatgcagat ttagttggcg
      601 gtatgaaata taatcatcgt gacttacgaa atgtattaga acgttcatct gccagagaaa
      661 cagcagcacg tgtagcggtc ggtgcactat gcaaagtttt attagaacaa ttagatatcg
      721 aaatatacag tcgtgttgtt gagataggtg gcattaaaga taaagatttt tatgattcag
      781 aaacatttaa agcaaacctt gatcgaaatg acgtccgtgt aattgatgat ggcatcgcac
      841 aagcaatgcg cgataaaatt gatgaagcga aaaacgatgg tgattcaata gggggcgtag
      901 ttcaagttgt agttgaaaat atgcctgttg gtgtaggtag ttatgtacat tatgatcgta
      961 aattagatgg aagaatagca cagggtgttg ttagtattaa tgcatttaaa ggtgtaagtt
     1021 ttggagaagg atttaaagca gctgaaaagc ctggtagcga aattcaagac gaaattctct
     1081 acaatactga attgggctat tatcgtgggt caaatcactt aggtggttta gaaggcggta
     1141 tgtcaaatgg aatgccaatt attgtaaatg gtgtaatgaa acctattcct acgttatata
     1201 aaccattaaa ttcagttgat ataaatacta aagaagactt taaggcaaca attgaacgtt
     1261 ctgatagttg tgctgtacct gcagcaagta tagtgtgtga acacgttgtt gcatttgaaa
     1321 tagcgaaagc attacttgaa gagtttcaat caaaccatat tgaacaactt caacaacaaa
     1381 ttgctgaccg cagacaatta aatgttgagt tttaacaacg agaacaattg aggtgtgatc
     1441 atgaaattac aaacaaccta tccttcaaac aattatccaa tatttgttga acatggtgca
     1501 attgaccata ttagcacgta tattgatcag tttgatcaaa gttttatatt aattgacgag
     1561 catgtaaatc aatattttgc tgataaattt aatgatattt tatcatatga aaatgtgcat
     1621 aaagtcatta ttccagctgg tgaaaagacg aaaacatttg agcaatatca agaaacatta
     1681 gaatacattt tgtctcatca tgtaacacgt aatacagcga ttatagctgt tggtggtggt
     1741 gcgacaggag attttgcagg atttgtagca gcaacactat taagaggtgt ccattttata
     1801 caagttccca caacgatttt ggcgcatgat tctagtgttg gcggtaaagt gggtattaac
     1861 tcaaagcaag gtaaaaacct tatcggtgca ttttatcgtc caactgctgt tatttatgat
     1921 ttagactttt taaagacgtt accatttgag caaatattaa gtggctatgc agaagtttat
     1981 aagcatgcgt tattgaatgg tgaatcagcg acgcaagata tcgaacagca ctttaaagat
     2041 agagagatat tacagtcatt aaaaggtatg gataaatata ttgctaaagg tattgaaacg
     2101 aagctagata ttgttgttgc agatgaaaaa gaacaaggtg tacgtaaatt tttaaattta
     2161 ggtcatacat ttgggcatgc tgttgaatac tatcataaaa taccacatgg tcatgcagtg
     2221 atggttggca ttatctatca atttatagtt gcgaatgctt tatttgattc taagcatgat
     2281 attaatcatt atattcaata tttaatacaa cttggctatc ctttagacat gataactgac
     2341 ttggattttg aaacgttata ccaatatatg ctaagtgata aaaagaatga taagcaaggt
     2401 gtacaaatgg tcttgattag acaatttgga gatatcgttg tacaacatgt tgatcaacta
     2461 acattacaac atgcatgtga acaattaaaa acatatttta agtaggtgaa tgaaatggta
     2521 aatgaacaaa tcattgatat ttcaggtccg ttaaagggcg aaatagaagt gccgggcgat
     2581 aaatcaatga cacaccgtgc aatcatgttg gcgtcgctag ctgaaggtgt atctactata
     2641 tataagccac tacttggtga agattgtcgt cgtacaatgg atatattccg attattaggc
     2701 gtagatatta aagaagatga agataaattg gttgtgaatt cacctggata taaagctttt
     2761 aaaacacctc atcaagtttt atatactgga aactctggca caacgactcg attgttagct
     2821 ggtttgttaa gtggtttagg tattgaaagt gttttgtctg gcgatgtttc cataggtaaa
     2881 aggccgatgg atcgtgtttt aagaccgtta aaaagtatga atgcaaatat tgaaggaatt
     2941 gaagacaatt acaccccatt aatcattaaa ccatctgtaa ttaaaggtat taattacaag
     3001 atggaagtgg caagtgcaca agtgaaaagt gccatcttat tcgcaagtct attttctaag
     3061 gaagcgacca tcattaaaga attagatgta agtcgaaatc atactgagac gatgttcaga
     3121 cattttaata ttccaattga agcagaaggg ttatcaatta ctacaatccc tgaagcaatt
     3181 cgatacatta aacctgcaga ttttcatgtt cctggcgata tttcatctgc agcgttcttt
     3241 attgttgcag cacttatcac accaggaagt gatgtaacaa ttcataatgt tggaatcaat
     3301 ccaacacgtt caggtattat tgatattgtt gaaaaaatgg gcggtaatat tcaacttttc
     3361 aatcaaacaa ctggtgctga acctactgct tctattcgta ttcaatacac accaatgctt
     3421 caaccaataa aaatcgaagg agaattagtg ccaaaagcaa ttgatgaact gcctgtaata
     3481 gcattacttt gtacacaagc agttggcacg agtacaatta aagatgccga ggaattaaaa
     3541 gtaaaagaaa caaatagaat tgatacaacg gctgatatgt taaacttgtt agggtttgaa
     3601 ttacaaccaa ctaatgatgg attgattatt catccgtcag aatttaaaac aaatgcaaca
     3661 gtggatagtt taacggatca tcgaatagga atgatgcttg cagttgcttc tctactttca
     3721 agcgaacctg tcaaaatcaa acaatttgat gctgtaaatg tatcatttcc aggattttta
     3781 ccaaaactaa agcttttaga aaatgaggga taatataaaa tggaagatat ctataaatta
     3841 atagacgata tcaatctaca aaaactagaa aatttagact ctcgtgttaa tgaagcaata
     3901 actactgaca acgatgacgc attatttatt ctaggagaga cactttacaa ttttggatta
     3961 atgccacaag gtttagaagt attccgcgtg ttatatcaca aatatccaga cgaaagtgaa
     4021 ttgctgattt attttattga aggtttaatg tctgaaaatc aaactgacga agcgttagaa
     4081 tatttaagtt atgttgaacc atcacctgaa aagttgatgt tagaagcaga tttatatcaa
     4141 caaattaata tgatggaagt tgctattgat aaattacaag aagcacttga actagagcca
     4201 aatgatccaa taatccattt tgcgttggct gaaatgttat attatgatgg tcaatattta
     4261 cgtgctacct ctgaatacga aactgtttta gaaactggtg aatatcaagt taatggtgta
     4321 aacttattct ctcgtatggc agattgtagc ttacaaagtg gtaactatag tgatgcgatt
     4381 cgcttatacg atgaaattaa tgaagatgaa atgacttcag aagattatct caaaaaagcc
     4441 atttcttacg ataaaaatga catcactcaa gaagcaatta aaataatgac tacattactt
     4501 tctaaagatc ctgattatat tcaaggctac ttgtatttac aatcattata tgaaaatgaa
     4561 aaaaattatc cagatgccat tgaaacgggt aaagagggat tacgactaag tcaattttat
     4621 aaagagttaa tgtatacaac tggatgtttg gaaatagaac acggcgatgc taatgaaggt
     4681 gtattattgt taaaacaagc attagaggtt gataatgctt accaagaacc attattgatt
     4741 ttaagcgatt tatatcgtaa tgaagaagat tatgaagcaa tcattgaatt attaacatat
     4801 gtagatgaag aagatttgga tcctacattc atctggcatt tagcgttcgc ctatggtcaa
     4861 gaagagcgag ataaagaagc tcaacatttc tttgaattag catatccaac tatgaaaaca
     4921 aatattgatt ttatgagtga ttattatttc tatttaattg aaattggtca aatggaacaa
     4981 gcaattacta ttttaaatca attgttggaa ctagaaccaa gtaacgaaaa ttggcatgac
     5041 gaattatcaa gattacaatc ttaaaatggg gtatttgatg tcagagacac tcaatcaaat
     5101 taaagaaagt tttattgaat atttactttt tcaatatcga tttaagtcgc gtattgcagt
     5161 ttgggtgctt aactatataa aagtgaatga ggcgaaattg gcaaacattc attttgtaga
     5221 tacaaaaatt aatcatcatg aaacattaga aattgcagaa gtgggaagtc acgcttctgc
     5281 aattcaattt actaagcata atataaagtt aatgaataca aatgaaatat ttgattatat
     5341 cgctaatcat aacggtgctt ttgatattca aattcacttc gcaaacgtgt caaagcgtga
     5401 gcaaaggcta gatgatttaa tagttgctca actaacggaa tcgcccagct atcaaactta
     5461 tcttcatgat ttaaatagta tggctataga tcggcataaa catgcgttat taatagatta
     5521 cttgctacat aatattgatt taagtttaca gatgaatgaa aagcaacgtt tttatcaact
     5581 aacgcaaatt ttaaatacac tcaagctagt aaataaacat aatcaatttg aggacttagc
     5641 agatgacgat taataaactt tggcaatata ctctttatca acgttcttgg ctaatgatgc
     5701 tactcatttg taatatatta gggatgattt atggttatat atggtatggt gaacaattaa
     5761 gtcatacgcc gtggcagttt aagatttttg ttccagatag cccaactgca atattattct
     5821 tagttatttc aatctcattg atattaatac aaaaacaaaa tagtattatc gatgcactgg
     5881 catttgtaac attattcaaa tatgggattt gggcagttat tatgaatata ttatttatta
     5941 ttgagcaagg tgatattaca gtaaatggcc tggtattaat gttttcccat agtataatgg
     6001 ctgttcaggc aatttacttc tatcctaggt ttaaacgttc tatgattgga attagtgtag
     6061 cgatgacttg ggtatttttg aatgattata ttgactattt tcaccttcaa tttccatatt
     6121 atgattttat aactacacat gtgtggcaaa ttggtgtatt atcatgttgt ctgagtgttt
     6181 ttggactttt gctttatatt gaattaaaca aattattaaa gtgtaaatga tttgataaaa
     6241 actgcaaatg acagtaaaat atttataaag gagagtgagt ttattgtctt caatttttac
     6301 tatgataatt tatttcgtaa tattaatggt gcttccttta tgggcacaac acaaagttaa
     6361 atctaactat gagaaatatt cacaagttag atcaactagt ggtaaaacgg gtcgtgaagt
     6421 tgcattagag attttacatg caaacggtat ttatgatgtc gaagttgtta aaggtgaagg
     6481 attcttaaca gatcattacg atcctaaaaa gaaagttgtt tcactatctc ctgcaaacta
     6541 tgacagacca tcagttgctg gaactgcaat tgcagcccac gaagttggtc atgccattca
     6601 agatcaccaa ggatattggt tcttaagatt cagagcagca ttagtacctg ttgcaaacct
     6661 agggagttca ttaagttata tgatcatcat gttaggtatt atcttaacag cgataggtag
     6721 tgcatttggt tcaactgctt tatggattgg tgctggttta atgtcattag cggtattatt
     6781 ctcaattgtt acgttaccag ttgagtttga tgcgagttca agagcgatga aacaaattac
     6841 ggcattgaac attgtaaatg aaaaggaata caaacatgcg cgtaaagtat tatctgcagc
     6901 agcaatgact tatgtagcag caactgctgt cgctgtagca gagcttgtac gtattatttt
     6961 gattgcgcgt tcaagtgatt aatcttatag attaacaatt aacttaaatt agacacttat
     7021 taactagcta atcacatttt gtgtggttgc tagttttttt gtgcttattt ttctaatggt
     7081 agattattca atatattatt ttattgtagt ggtacgaatc ataaagttat aatctcaatt
     7141 tcaattgatt taatttagtt aggaatgcat tacatttata taaactgaat tattaaacta
     7201 ctaaaaaaaa caacttgtca tctagtttag ctagagtcag tagattgcac taaacatgca
     7261 tgagacttca aattgatata tactatatta gaaatgcaat ataaggagaa cgtagaaatg
     7321 aaatcaatgg tagaaatgca acgtgaagtt gatgaatata ttggacaatt taaaacagga
     7381 tatttttcac cattagctaa cttagctaga ttgactgaag aagtgggcga acttgcacgt
     7441 gaaataaatc atacctatgg tgaaaaaaag aagaaagatt cagaggaagc aaatacgatt
     7501 aaagcagaat taggtgataa tttatttgtg ttgttatgtt tagcgaattc aatgggaata
     7561 gatatgacag agagctttaa tgaaacaatg gaaaagttta atacaagaga taaaaatcga
     7621 ttcaaaagaa agtgaatata aaaatatatg ctttatttgg atttgattaa gattaaaaac
     7681 atagactaat gaattgcatc gcatgaaatc atattatttt taaagtaagt ataactagat
     7741 aaatgattat atatacaaca cctgtttttt agatatttga aattataaac tcaacattac
     7801 tataaactta aatttaaaca attatctacg catcgagaat aatagaaaga gaaatagaaa
     7861 gaaaggatgg gcaagcgaat gaagataggt ataacatgtt acccgtccat gggtggctct
     7921 ggaattattg ccacagaatt aggaattaaa ttagcagagc gaggtcacga agtacatttt
     7981 atcacttcaa atatcccgtt tagaataaga aaaccattac cgaatatgat ttttcatcaa
     8041 gttgaagtga atcaatatgc agtttttcag tatccaccgt atgatattac gttaagtact
     8101 aaaatcgctg aagttattaa agaatatgat cttgatttgt tgcatatgca ctatgctgta
     8161 cctcatgcaa tttgtgggat tttagctcgt gagatgtcag gtaaagatat aaaaattatg
     8221 acaacgctac acggtactga tattactgtt ttgggctatg atcattcact ccaaggtgca
     8281 attaaatttg gcattgaaaa aagtgatatt gtgacaagtg tgagtaaatc tttagcgcaa
     8341 gaaacacatg aaattatcga aactaacaaa gaaattatac cgatttataa ttttgtacgt
     8401 gaaaatgagt ttccaactaa acataacaca gcattaaaat cacaatttgg tatagcacca
     8461 gatgaaaaag tgttaataca tgtatcgaat tttagacaag taaaacgcat agatactatt
     8521 atagaaacat ttgcaaaagt acgtgaaaag atacctagta aattaatttt acttggtgat
     8581 ggtcctgaat tagtgccaat gcgtcaaaag acgaaagagt taaacgtaga agaagatgtt
     8641 ttatttttag ggaaacaaga ttgcgtaagt gaattttacc aattatctga cttagtatta
     8701 ttattaagtg aaaaagaaag ctttggacta actttacttg aggctatgaa aacaggcgtc
     8761 gttccaattg gttcaaatgc cggtggaatt aaagaggtta ttaaacatgg tgaaactgga
     8821 tttgtcgtag atgtgggtga tagtgattca gcgagtgact atgctatccg attacttgaa
     8881 gacaaggtgt tatacaacaa acttcagaaa aacatgctag cagatattgc tgaacgtttt
     8941 ggatcagaac tcattacaga tcaatatgag tattactatc aaaaaatgct aaatgagcat
     9001 aacaaaagta aaggcgagta ataatatgga taaatcatta tttgaacagg caaggcctat
     9061 attagaacaa attcaagaca atggttttga agcatattat gtaggtggct ctgtaagaga
     9121 ttatgtcatg ggaagaaata ttcatgatat agatatcaca acaagtgcaa cgccagatga
     9181 aatagaatct atctttagtc atacgatacc tgtaggtaaa gaacatggca ccataaatgt
     9241 agtttttaat gatgaaaatt atgaggtgac aacattccgg gctgaagaag attatgtcga
     9301 tcaccgtaga ccaagtggtg ttacatttgt tcgtgattta tacgaagatt tgcaacgaag
     9361 agatttcacg atgaatgcga tagcaatgga tacagcaaac aaattgtatg attattttga
     9421 tggtcaacaa gatattaata atcgaataat aagaactgta ggtatagctg aggaacgatt
     9481 ccaagaagat gctttacgta tgattcgatg tttaagattc cagtcacaat tatcatttga
     9541 tattgcaacg gaaacattcg aagcgatgcg tacacaaatg gcagatatta aatttttatc
     9601 aattgagcgt atagtgattg aactaactaa attaatgcga ggtattaatg ttgaaaagag
     9661 ttttaatcat ttaaaatcgc tgaaagcatt taattatatg ccatatttcg aacatcttga
     9721 tatgaatcaa attaatgtga ctgaaccaat tgatttagaa ttgttgattg ctatagtatc
     9781 agttaaattt gatattaatt actcattgaa gcctttaaag ctaagtaacc gacaagtaaa
     9841 agatatcaat caatatattc aaattatgaa tgcattacca agtattatta caaaagaaca
     9901 attaaaaatg tttgtttatg attatgatac gaatctcatt aaaaatgtaa tggttgcagc
     9961 agacgtgtta aaggcaaatg atattcaagg acatgaacca ttaatcgtta accttcaaac
    10021 gattgatgaa acattacatc gtttacctat gcataataga aaagacatga tggttaatgg
    10081 cggtgtactt atggcacatt taaatgctaa aagtggtccg tggttaaaag atctgctaag
    10141 acaaattgag atagcgattg taactggtaa agtaagcaac gaagaaactg aaattttgaa
    10201 atgggtggat aatcatgtca aaatatagtc aagatgtact tcaattactc tataaaaata
    10261 aaccgaatta tatatctgga caaagcattg cggaatcact taatatatcg cgcactgcag
    10321 taaaaaaagt gattgaccaa ttaaagttag agggatgtaa aatagattca gtaaatcata
    10381 aaggacattt attacaacaa ctcccagata tttggtatca aggtataata gaccaataca
    10441 caaaaagttc tgctttgttt gattttagtg aagtatacga ttcaatagat tctacacaac
    10501 ttgctgcgaa aaagtcactt gttggaaatc aatcttcatt ttttatcttg agtgatgaac
    10561 aaacgaaagg tcgcgggcga tttaatagac attggagttc ttcaaaaggg caaggactat
    10621 ggatgtctgt tgtattaaga cctaacgttg cattctcaat gatatctaaa tttaatttat
    10681 ttattgcatt agggataaga gatgcgattc aacattttag tcaagatgaa gtcaaagtga
    10741 aatggccgaa tgatatatat attgataatg gtaaagtgtg tggtttctta actgaaatgg
    10801 ttgctaataa tgatggtata gaagcaataa tatgtggtat aggtattaat ttgacgcaac
    10861 aactagaaga ctttgatgaa agtattagat atagagcaac aagtatacaa ttacatgata
    10921 aaaataaatt agatagatat caatttttag agagattact tcaagaaatt gaaaaaagat
    10981 ataatcaatt tttaacgtta cctttttctg aaattcgtga agaatatatt gcagcttcta
    11041 atatctggaa tagaacgttg ctatttacag aaaatgataa acagtttaaa ggacaagcaa
    11101 ttgatttaga ttacgatggc tatctaattg ttagagatga agcgggtgaa tcacaccgtt
    11161 taattagtgc agatatagat ttttaacact aaagcaagga gagatagcta tgggtatggc
    11221 aacctatgcc gttgtggatt tggaaacaac aggcaaccaa ttagattttg acgatatcat
    11281 tcaaattggt ataacgtttg tgcgcaataa ccaaattatt gatacatatc attctatgat
    11341 tagaacaaat ttagagattc cgcctttcat tcaagcgtta acatccattg aagaaaatat
    11401 gctacaacaa gcgccatatt tcaaccaagt tgcagaagaa atctacgata agataaaaga
    11461 ttgcattttt gtcgctcata atgtagattt tgatttaaat tttattaaaa aggcatttaa
    11521 agattgtaat attcaatatc gtcccaaaaa agttatagat acactagaaa tctttaaaat
    11581 agcttttcct acagataaaa gttatcaatt aagtgaatta gcggaggcac atggtattac
    11641 cttagcaaat gcccatcgag ctgacgaaga tgccgctact actgctaaat tgatgatatt
    11701 agcttttgaa aaatttgaaa agttaccgtt agatacttta aagcaattat attatttaag
    11761 taaacagttg aaatacgatt tgtatgatat tttctttgag atggttaggc aatatgatgc
    11821 aaagccattg gataaatcgt acgaaaagtt cgaacagata atatatcgta agcaagttga
    11881 ttttaaaaag ccgacaacaa attataatgg cagcttgaag tctttatata gcaaagctgt
    11941 agatcaactt ggcttaacat atcgaccgca acaattatat ttagcggaaa ctatattaga
    12001 tcagctcatg catagtgaaa aagcaatgat tgaagcatca ctaggcagtg gtaaatcatt
    12061 agcatattta cttgcggcct tgatgtataa tattgaaact gggaagcatg tcatgatttc
    12121 taccaatact aaattactgc aaagtcaatt gttagaaaaa gatattccag caatgaatga
    12181 agcgttgaat tttaaaatta atgcactatt gattaaaagt aaaagtgatt acatttcatt
    12241 gggacttatt agtcaaattt taaaagatga cacaagtaat tacgaagtga atatcttgaa
    12301 aatgcaattg ctgatttgga ttacagagac gccgtccgga gatatacagg aattaaattt
    12361 aaaaggtggt caaaagatgt attttgacca gaaaattgaa acatatgtac cggcacgtca
    12421 cgatgttcat tattataatt ttattaagcg aaatgcacag aacattcaaa ttggtattac
    12481 aaatcatgca catttaattc attctgatgt tgaaaattca atatatcaat tgtttgatga
    12541 ttgtatagtg gatgaagcac atcgcttacc agactatgcc ttaaatcaag tgacaaatga
    12601 gttgagttat gctgatatta aatatcagct tggtttgatt ggtaaaaacg aaaatgaaaa
    12661 attgttaaag gcaattgatc aactggaaaa gcagcggatt ttggaaaaat tagatattgc
    12721 accaattgat atttttggct tgaaggctag tatgaatgag attcatgagt taaatgagca
    12781 attgttctca acgatattta cgattatcaa tgattctgat gtttatgatg atgatattca
    12841 tcgcttccat aatgtattta cttttgaaac aaaagacatt ttgaaggatt tacatgcgat
    12901 tatcgataag ttaaataaga cgctagaaat attcaatggc attagtcata aaacagttaa
    12961 atcattacgg aaacagttgt tgtatttaaa agataaattt aaaaatatcg aacaaagttt
    13021 aaaagctgga catactagtt tcatttcaat taaaaatcta tctcaaaaat caacaatacg
    13081 tttatatgtg aaagattacg ctgtaaaaga tgtattaacg aaacaagtat tggaaaaatt
    13141 taaatcactt atttttatat ctggtacttt aaaatttaat cattcgtttg atgctttcaa
    13201 acagttattc aacaaagatg ttcactttaa tacatttgaa gttaacacat cgttacaaag
    13261 tgcaaaaaat acaagtgtat ttataccgag tgatgtagca tcttatcaat ataaaaatat
    13321 agatgagtat gtagcatcga ttgtaagcta tattattgaa tatacgacta taacgtcatc
    13381 aaaatgttta gtcttattta cgagttataa aatgatgcat atggtacaag atatgctaaa
    13441 tgaattgcca gaatttgaag attatgttgt attaacacaa cagcagaacc aaaattataa
    13501 aatagtacaa cagtttaata attttgataa agccatactg cttggtacgt caacattttt
    13561 tgaaggcttt gattttcaag caagtggtat taaatgtgtg atgatagcga agttaccgtt
    13621 tatgaacaag cataatgcaa aatattggct tatggattct gaattcactt caactttcaa
    13681 ggaatatgta ttacctgatg cagttacacg ttttagacaa ggacttggaa gattaattag
    13741 aaatgaaaat gatcgcggca taatagtttc atttgatgat cgactcataa acagtaatta
    13801 taaaaacttt tttgaacaaa cacttgaaaa ttatcgtcag aaaaaaggcg acattcaaca
    13861 gtttggaaaa ttattaagac aaattcaaaa aaagaaaaag taaaatacaa agtaaaattc
    13921 ttgtgacatg acatgtgcta tgttctgtta caagaatttt tgtttggtcg tcaaaatttg
    13981 agttttataa ttaaacttga atgttagcac aaaaaacgac cttcatacta ataatattga
    14041 ggctttatta tatattttgt ctgaaactga tgattgtatt gtcttaatat aaattaatgt
    14101 tcaaatctgt gcttaaatca ttaccattta cacgttatct tttagtctag ttctagaact
    14161 tgaaagcgga ttttgttaaa atgaatgagt attgtataac gaaaaaataa ataggagaat
    14221 ggttatgaaa acaacgatta aacaagcgaa agatcattta aaccaagacg ttacaattgg
    14281 tgcttggcta acaaataaac gttcaagtgg taaaatcgcc tttttacaat tacgtgatgg
    14341 aacaggcttt atgcaaggcg tagtagttaa atcagaagtt gatgaagagg tattcaaact
    14401 tgcgaaagaa attactcaag aatcatctct atatgttaca ggcacaatta cagaagataa
    14461 tcgttctgac ttaggatacg aaatgcaagt gaaatcaatt gaagttattt cagaagcgca
    14521 tgactatccg attacaccta aaaatcatgg tacagaattc ttaatggatc accgtcattt
    14581 atggttacgt tctaaaaaac aacatgctgt aatgaaaatt agaaatgaag ttattcgtgc
    14641 aacgtatgaa tttttcaaca aagatggatt tacaaaggtt gatccaccaa ttttgacagc
    14701 aagtgcacca gaaggtacaa gtgaattatt ccatactaaa tactttgatc aagatgcgtt
    14761 tttatctcaa agtggtcagt tatacttaga agctgcggca atggcacacg gaaaagtatt
    14821 ttcatttggt ccaactttca gagctgaaaa atcaaaaaca cgtagacact tgatcgagtt
    14881 ctggatgatt gaaggggaaa tggctttcac aaatcatgct gaaagtttag aaattcaaga
    14941 acaatatgta acacatgtag taaaatcagt tttagaaaat tgtaaactag agttgaaaat
    15001 gttagagcgt gatacatcaa aacttgaaaa agttgcgaca ccattcccta gaatttcata
    15061 tgatgatgca attgagttct tgaaagcaga aggctttgat gatattgaat ggggcgaaga
    15121 ttttggtgcg ccacatgaaa cagccattgc taatcattat gatttaccgg tatttattac
    15181 taattatcca actaaaatta agcctttcta tatgcaacca aatcctgaga atgaagaaac
    15241 tgtattatgt gcagacttaa ttgcacctga aggatacggt gaaattatag gtggttctga
    15301 acgtgtggat gacttagaat tgttagaaca acgcgttaaa gaacatggat tagacgaaga
    15361 agcatatagt tactacttag acttacgtcg ttatggtagt gtaccacact gtggatttgg
    15421 tttaggttta gagcgtacag tcgcatggat ttctggtgtt gaacacgttc gtgaaacagc
    15481 gccattccca agattattaa accgtttata tccataagtt ttagggtcgt tacttaaaat
    15541 ttatattatg ataataaaaa gaatcgtcca gcctatatta atagctgggc gatttatgct
    15601 tggaaaatga agaatgataa cggcttatat taatcatgaa caaacttttg tttagtaact
    15661 atatttggaa aataaatagc tcgtctgatt atacataaag ttcattgtta aaatctagtt
    15721 ttaaaaaaat actgttattt aaaatttgtt tgctactagt taaatatatt aagcatttta
    15781 gtcgaaaatt aaagaaaatg atttatacta ttaagtaatg gttaaattta aaagggggca
    15841 cgaacatgga taaatatcaa ttaaaagcaa gacctgtagt gatacgaaga gaattattag
    15901 accattacag tgacttaggt ttagatgagc aagatttagt cattttgctt aaattgattt
    15961 atgcttccga aacatcaaac aaacagcctt caattgaatt acttcagaag ggctcaacta
    16021 tgcaaccacg tgatattaca atggttattc aaaatttgat tcaacgtgaa ttgctagaat
    16081 tacaagttca aaaagatgaa gaaggtagat ttactgaata tatgaatcta gatccttttt
    16141 tcgaaaaatt aagtcatata ttgaagcaac aaagtatgga aacgaaagag caaaatagta
    16201 aagaaaaatt taaacaatta tttagagttt tagaggatac atttgcaagg ccgttatctc
    16261 catatgaaat agagacgtta aatcaatgga ttgatgttga taaacacgat actgcaatta
    16321 tacaagcggc attagacgag gcgaatagtt tgaataaact tagctttaaa tatatggatc
    16381 gtattttact taattggaag aaaaacaatg tcaaaacaat tgacgattct agaaaaataa
    16441 gagaaaaatt taataaaccg aaaatgacgc acacagttaa aacggtacct aaatttgatt
    16501 ggttaaacgg ggagaacctt gatggtaagt aagaaaaaag cattagaaat gattgatgtt
    16561 atagcaaata tgtttccgga tgcagaatgt gaactaaaac atgataatcc gttcgaatta
    16621 actattgctg tattattgtc agcgcaatgt acagacgttc tagtaaatag agtaacaact
    16681 gagctgttta aaaaatacaa aacgcctgaa gattatttgg cagtcagtga tgaagaactt
    16741 atgaatgata ttcgttctat cggtctgtat cgtaacaaag ctaagaatat taagaaactt
    16801 tgtcaatctt tgattgatca atttaatgga gaaataccac aaacacataa ggaattagaa
    16861 agtttagcag gtgtaggacg taaaactgct aatgtagtca tgagtgtagc atttgatgaa
    16921 ccttcattag ctgttgatac gcatgtagaa cgtgtttcta aacgcttagg tattaatcgt
    16981 tggaaagata atgtgagaca ggtagaagac cgtttatgtt ctgtcatacc tagagataga
    17041 tggaatagga gccatcatca actcattttc tttggaagat accactgttt agcccgaaaa
    17101 cctaaatgcg atatttgtcc attattagaa gattgtagag aaggacaaaa acgttataaa
    17161 gctagtttga aagaagcgtg ataatatgac aaatattaca aaagaggtat ttgataattt
    17221 agaacaaaag attgatgtat ttgccaaaaa taagacatta ggttctagtg aagcaaaacc
    17281 ttacttggat gaataccata gtaaaattat tgattatttt aagcaggtta atgacataac
    17341 tggcaatatt gattttgata atttaaatca atatcctgtt gtgccaatga attttaaaga
    17401 aagatatgat tatatgattg aacgta
//
LOCUS       DATTQF010000044        17370 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.044, whole genome shotgun sequence.
ACCESSION   DATTQF010000044 DATTQF010000000
VERSION     DATTQF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.044"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            129..1406
                     /locus_tag="VB241_001256"
     CDS             129..1406
                     /locus_tag="VB241_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1244559.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            1883..3706
                     /locus_tag="VB241_001257"
     CDS             1883..3706
                     /locus_tag="VB241_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEP1244560.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMILGTLTATTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGVLATMMVLITILLSKNKAEEQAIQNDFKLISKLLHAYN
                     DKSAFSQVAKTAVDSFKASDKLLITSNSSSDKLSGRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIITQRDDRNKNVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEAEMRKPLYSKRIYQNLTLDSIVFRNTLRYAAIMMIAIFIALMFDFEKAYW
                     IPLSAHTVLLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYTIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPYVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            3845..4261
                     /locus_tag="VB241_001258"
     CDS             3845..4261
                     /locus_tag="VB241_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEP1244561.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKPEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            4492..5184
                     /locus_tag="VB241_001259"
     CDS             4492..5184
                     /locus_tag="VB241_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP1244562.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNKAETEKYRDLLKECATEITEVE
                     NKLQQIHSEITE"
     gene            5349..6080
                     /locus_tag="VB241_001260"
     CDS             5349..6080
                     /locus_tag="VB241_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1244563.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLD
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSILSS
                     ILMKNKWPFLIALPFMIGYGIGITIYYQRKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKTHMGTE"
     gene            6267..6947
                     /locus_tag="VB241_001261"
     CDS             6267..6947
                     /locus_tag="VB241_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1244564.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            6972..8525
                     /gene="gntK"
                     /locus_tag="VB241_001262"
     CDS             6972..8525
                     /gene="gntK"
                     /locus_tag="VB241_001262"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEP1244565.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDRYIIDYSMASATGMFNLETLDWDVGALELLGISEEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     KHMAENKTQ"
     gene            8642..9990
                     /locus_tag="VB241_001263"
                     /pseudo
     CDS             8642..9990
                     /locus_tag="VB241_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
     gene            10372..10474
                     /locus_tag="VB241_001264"
                     /pseudo
     CDS             10372..10474
                     /locus_tag="VB241_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10458..13355
                     /gene="fnbA"
                     /locus_tag="VB241_001265"
     CDS             10458..13355
                     /gene="fnbA"
                     /locus_tag="VB241_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HEP1244566.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKVSETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEVVKEEANPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTALESKPRVTRSTDVAEAKEASDAKVETGTDVTSKVTVEDESKIEAPKGNNVQ
                     PHEGQRVVLKYKLKFQDGLKTGDYFDFTLSNNVNTHGVATTRKVPDIKNGSLVMAKGQ
                     VLDNGRIRYTFTDYIKDKVNVTANLEINLFIDPKTVQSNGQQTITSKLNGKETSGTMQ
                     ITYKDGVKNQYTNVNGSIETFDKEKNKFTHVAYIKPINGNNSDSVTVTGMLTQGSNEN
                     GTQPNVKIYEYVGVENGLPQSVYANTVDSTQLKDVTNQMGDKLKVQNNGSYSLNFDKL
                     DKTYVIHYTGDYLNGTSEVNFRTQLTGYPENRYKTYYYYNNGYTLTWDNGLVLYSNKA
                     NGDGKYGPIVDSNNFEFSEDSGNGSISGQYDAKQIIETEENQDNTPLDIDYHTAIDGE
                     GGYVDGYIETIEETDSSAIDIDYHTAVDSEARHVGGYTESSEESNPIDFEESTHENSK
                     HHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESN
                     LIELVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIK
                     SELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHNEIIEEDT
                     NKDKPNYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPGTPTP
                     PTPEVPSEPGTPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVK
                     AVAPTKQKQAKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            13535..14401
                     /gene="galU"
                     /locus_tag="VB241_001266"
     CDS             13535..14401
                     /gene="galU"
                     /locus_tag="VB241_001266"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP1244567.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIIEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(14528..14836)
                     /locus_tag="VB241_001267"
     CDS             complement(14528..14836)
                     /locus_tag="VB241_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244568.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     TQIAYIFALCISLSAVFLMSAILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(14980..15246)
                     /locus_tag="VB241_001268"
     CDS             complement(14980..15246)
                     /locus_tag="VB241_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244569.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(15530..17338)
                     /locus_tag="VB241_001269"
     CDS             complement(15530..17338)
                     /locus_tag="VB241_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP1244570.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIKRANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     IIVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYSSDGAQLSTDASE
                     LVSRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYQLDKDTTSQIDSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
BASE COUNT         6181 a         2615 c         3334 g         5240 t
ORIGIN      
        1 aatattataa aaaataactt tggtacaatt ttgtgacaaa gtaggggtgg acatgccaga
       61 tgtccgaaaa ttttaacgtt tcagggtaag ttttaaaaaa ttaattaatt agggaagtag
      121 gaatagttat gaaggaaaaa cgaactaacg taaggtggat gttcgcgctt gcgttcttct
      181 ttattggggt tattgcgtat atggatagag caaacatttc atatatcgct aagcaaatga
      241 tggatgattt agggatgaca aaaccacaat ttggtttatt ggcatcattc ttctctctag
      301 gttatgcatt aatgcaagta ccatcgggga tgttggctga aaaattcggt ccacgtaaga
      361 tgattacaat tgcattagtt tggtggagtg catttacaat cttaacgggt atgattaaga
      421 accacggttt aatttattta gtgagattct tatttggtgt aggtgaggcg ccaatgtacc
      481 cttctaatgc tgtgtttaac tcattttggt tctctaaaaa tgaaaaaggt agagcatcaa
      541 gtgcattatt agcaggatca tatttcggac ctgtattagc accaatagtt acaattgcta
      601 ttgttaacgc atttaactgg caagcagtat tttatatttt tggtgcagta gggattttaa
      661 tggctgtatt atgggcgatt attgctaaag acttacctga gcaacataga atggttaatg
      721 aagcggagaa acgtttcatt atggaaaacc gtgatatcgt agctactgaa aagtcatcac
      781 caccatggaa tgatttcttt aaacgtttta gcttctatgc aattgcaatt caatactttg
      841 ttgtacaatt tatcattaca ttattcttaa tttggttacc gacgtattta acagaagtat
      901 tccacgttaa ctttaaagaa atgagcatta gttcattacc ttggttatta atgttcttct
      961 taatcttatc agcgggtgca atttctgacc gtgtattagg attaggtcgt tcaaaattcg
     1021 tagctagagg tgtaattgca atcgcaggat ttattgtatt tgcagtttca attatctttg
     1081 ctgtacgtac aggaaattta tatgtaagta ttttctggtt atcactaggt cttggtggta
     1141 tcggtatttc aatgggtatg agttgggcag cagcaactga tttaggacgt aacttctctg
     1201 gtacagtatc aggatggatg aacttatggg gtaatatagg tgcattaatc agtccgctat
     1261 tagcaggtct attcgtagaa catttaggtt ggacaatgac attccaattg ttaatcgttc
     1321 cagcagtaat cgctgtgatt atgtggttct atgtgaaacc agatcaacct ttaattgtta
     1381 gtgatgataa agcaatagaa aaataattta atcatgcaat aagctttcac atagttgggg
     1441 cttattgctt ttttcgttga aattgaaaaa ttttaaaaca gatatggttt aagatgaacg
     1501 ggaagttatt aaaataatat atgtagaaga aataaagttt taaaacctta gtcaggtaac
     1561 tattgtaaaa gtacaaataa attttaacta gcgcaaaggt gggcgaccaa aggttcaacg
     1621 atgttaaata acattagaaa ataattttaa ttggacttta aaagttttta aatttagata
     1681 attgagcata aggtgttata atgacatatg ttgcgtaatt gaaatttata gcaacatact
     1741 caaattaact atgctaataa aatgattatg gaaatatttt gacgaggaag ggagaagtcg
     1801 aaatgacatc tttttgacat cacttataaa aattaatcga cttaacttag acttttataa
     1861 agggataaga caaggactgt taatgattat tcctgcaatc atcggttact tatgtggtaa
     1921 tttccaattt ggattattag ttgcaaccgg aactctagca catatctacg tttttaaagg
     1981 tccgtcgcga tctaagctgc gaactgtaat aatttgtaat ttagcgtttg caatatgcat
     2041 gatacttggt acgctaacag ccacaacgcc gctcgttttt ggaatgacat tattaattgt
     2101 tacggttata ccattttata tatttaccgc cttgaaaatt gctggaccgt catcgacatt
     2161 cttcattgtg acattcagtt tacccattaa cttacctata gcacctgaag aagcattata
     2221 tagaggcttt gcgattttag taggtggtgt acttgccact atgatggtac tcatcacaat
     2281 cttattgtct aaaaacaaag ctgaagaaca agccattcaa aacgatttta aactcatatc
     2341 taagttattg catgcttaca atgataaatc tgcattttca caagtagcaa aaacagcggt
     2401 ggatagtttt aaagcgtcgg ataaactact catcacttct aattcaagta gtgataaatt
     2461 aagcggacgt ttccaaaaat tattgttatt acacacatca gcacaaggga tatattctga
     2521 attattagag ctgaatgcta aacaaatcag accattgcca gatgagttaa ttgaaatgat
     2581 ggatcatatc attacacagc gagatgatcg caataaaaat gtgagatatt ggcgcaaaga
     2641 agtaacagta acagaggaat ttcaaaactt attcaaccat atcttgaaaa ttgacgaaat
     2701 ggtgcatgca aatgaagcac gtatcgcata tgaagctgaa atgcgaaaac ctttatatag
     2761 taagcgtatt tatcaaaatt taacattaga ttcaatcgtg tttagaaata cattgaggta
     2821 tgcagcgatt atgatgatag cgatatttat cgcgttaatg ttcgattttg aaaaagcata
     2881 ttggatacct ttatccgcac atacagtatt attaggaaca tcaactatac atgcaattga
     2941 gagaggtatg gcacgtggat taggcaccat tttaggtgtg ctagtacttt cagttatatt
     3001 gttgttttca ataccaacac ctgttgcagt cattttaatg ggcattgcag cattatttac
     3061 tgaagcgttg gtgggagcaa attatacaat tgcagtagtt tttattacaa tacaagttat
     3121 tttaatgaac ggattagcat cacagaactt aacaattaac attgcgtttc caagagttat
     3181 tgacgttgca atgggtattg tgattgcaat cataggttta tttgtccttg ggcaacgtac
     3241 cgcatccgca ttgcttcctt atgtaatggc tgaagttgtt cgtaaagaag caacgctctt
     3301 ccattattta ttttctgaaa accaatataa agatgatgtg tatcagaaaa acacggctat
     3361 gaacctatct gtgaaattaa ataatatgac gcaagtttat aatgcagcta atggtgaatt
     3421 atttagtgat aaaacattga ttcaaaatta ttatcctagc ttattcgcat tagaagaaat
     3481 tagctttatg ttaaatagag ccatggctaa tgaagataga cttacaataa atgaacaatt
     3541 aatgggcgag tatttagcaa catttgaaaa catcgctaag catttagaat taaatacaga
     3601 attagaaata aagatattac cagatttacc acagtataat tatattcaat cagcaatgat
     3661 gaatattcaa cacaatggtt ttcgtgagag agacaagaac gtttaaaata aattatttat
     3721 caggtcagac taacgtttgt ctgaagttat tatgacggga gtctaaaaaa ttgtgtttaa
     3781 tatgtttcat tttataatta tggtggcatt taacatgaat tataacatat tggaggttaa
     3841 ggaaatgccc tttacaatga aaggttaccc cgaaaaatgg aaagaatttg aagtaattaa
     3901 acggcaaaag gcgatagaaa ttgcgaatgc aatgttaaaa gaaggatatc ttgaaaaaga
     3961 tgttgttcct attgcggcga aaaaagcgaa agattggtat cgagcgttat cgaaagaaga
     4021 aatcaaagca ttagaagaag agaacttcgt acaattccat acagcaacaa tggaaaaacc
     4081 agaggtggat agtgaatatg caacaactga taatgaagaa catattcaag aacaatcaaa
     4141 tcaagataaa tcgtactttg atagcgaatc aattggcaac caaagtggtg gcgttcgaca
     4201 aagagaaccg cttatggatg gtccgcatcc aaaagttgtg gaatcattta aagacgtata
     4261 acatctctat caattaagca cttgtaccta tataacggta acaggtgttt ttttgcgtta
     4321 aatcatttta aaattacttc ctataattga aggttttgat acataattcg aattgtttga
     4381 gtttctttgt gctgtttcag tatcagccca aagttcgata gtgtattagt attatcttaa
     4441 taaaatgtta ggtacaataa agatgattat atatcggagg taagtataaa aatgtatgta
     4501 gatcgaaaac catcactata tttagaggat ttgcgacatg attttaaaaa tagtttaagt
     4561 aaatttgaaa atggtgatga agcatttgat acgttattag gtttcgtaga gttagatcat
     4621 atttattcat cagcactaaa ggaaataagc acaaaactga gtattttaga tgacaatttc
     4681 aatcacattt ataaacacaa tcctatacat catatggagc gacgtgtgaa agaaatgcgt
     4741 agtttaatag aaaagcttaa tcgtaaagga ttacagatta gcgcagaaac tgccaaagaa
     4801 catatactgg atattgcggg aattcgcgta gtatgtaatt acttagatga tatttatttg
     4861 attgaagaga tgttgcttaa acaagaagac gtacaattga taaaacgtaa agattatatt
     4921 cagcacccta aagaaaatgg ttaccgcagt ttacatatcg ttgtatccat tccagtcttt
     4981 ttagcagaac gcgttgaggt attgcctgtt gaaattcaaa ttagaacaat aggtatggat
     5041 atgtgggcaa gtttagaaca taaaatacgt tataaaaaca aggcagagac ggaaaagtat
     5101 cgagatttat tgaaagaatg tgcgacagag attactgaag ttgaaaataa attacaacaa
     5161 attcattctg aaataacaga gtagacgttt agtaggtgtg ttgcttaatt tgagtgattt
     5221 tgtaaatcaa catggtatga aatcacaaaa ataagacgcc tagtataata aatttcttaa
     5281 aaatgtggaa attatacttg ctcgtgacgt cgggtcatca actatggtga ttgtaggagg
     5341 tggaatctat gtctaactat tcgactggag aactcgcaaa attatgcaat gtgacaacac
     5401 gaacgattca atattatgat cgcaaaggta ttttgaaacc acaaggattt acagaaggaa
     5461 agcgtcgtgt gtatacagaa caacagcgac aaacattaga gttaatctta ttacttaaag
     5521 atttaggttg tgcgttaagc gatatagata tgttgctaaa aggtgaaggt actttgaaga
     5581 cactcaatac cttactaact atgaaacaac aagaaattaa tcaacaagtc aaacagcaac
     5641 aagcggtatt agacaaaatt aaaaatgttc aatattacgt aaatgaagcg tcgacgtctc
     5701 caatcacaca tttaaaagac atagagcatg tcatgagtaa atcagctgaa atgaaaagta
     5761 ttcgtcgtaa catttggatt agtgctggta ttataggaat aattcaatat tctagcattt
     5821 tgagttcaat cttgatgaaa aacaaatggc cgttcttaat tgctttacca tttatgatcg
     5881 gttatggcat tggtattact atctactatc aacgcaaagt tgcctattta tgccctaact
     5941 gccagcatat attctcaccg tctttgtggg cagttatcaa agcgaaacat actgcgacaa
     6001 cacgtcgatt cgaatgtcca aactgtcatg aaacgcatta ttgcattgaa gtacctaaaa
     6061 cacatatggg tacagaataa ttagacacat cccacataca acataacaat taaatcattc
     6121 gtgtgatagt gaccattccc tttaaattta cattgaagag gggtggtcac tattttatta
     6181 cgcgtaaaga aagcgcttgc aaacacttaa gaactatatt aacatacttg tatacaagta
     6241 tgtcgtgtaa tgaaggagtg gctttgatga tgtatggata tccagagaaa tggttggaag
     6301 gtatgacaac tggagaaggt atcgcagcag aattacgctt aggcattgtg aatggtcaca
     6361 tagctgaagg tacgttactc actgaaaatc aaatggcaaa gcaatttaat gtgagtcgtt
     6421 cgccaattcg agatgcattt aaattattgc aacaaaatca actcatccaa ttagaaagaa
     6481 tgggtgcaca tgtgttgccg tttggggaac aagaaaagaa agaaatgtac gatttgcgac
     6541 tgatgttaga gtcatttgca ttttcaagag ttaaaaatca agagcgacta cctatcgtaa
     6601 aagaaatgaa gaaacaactt gaaatgatga aagtggcagt aaaatttgag gatgcagaat
     6661 catttacgaa gcatgacttt gaatttcatg aaacattaat caaagcatct aatcatcaat
     6721 atttaaactc attttggagt catttaaaac cagtaatgat ggcgctcgtt ttaacatcca
     6781 tgcgacaacg tatgcaacaa aacccgcaag attttgaacg catacatcat aatcatcaag
     6841 tgtttattga tgcagtggaa caatacgaca gtcaaatttt gaaagaagcg tttcatttaa
     6901 atttcgacga tgttggtaaa gatattgaag gattttggtt gaattaaaat gaaaagaaag
     6961 aaggatttgt catgaaatat atgattggtg tcgacattgg aacgacgagt accaagtcag
     7021 tcttatatga tgaaaatgga actttcatca tgaaacatca aatcggctat gatttacaca
     7081 caccaaacgt tgatgtctca gaagaaaacc cagatgaatt atttgatgcg gtattaatga
     7141 caattaaata cataatgagg gaatcgaaag ttaatcaaga tgatattaaa tttgtgtcat
     7201 ttagtgcgca aatgcatagc ttgattgcga tggatcagca acatcaaaga ttaacaaata
     7261 atattacttg ggcagataac cgcgctgcaa aatatgcaac agtaataaat gaagtgcatg
     7321 atggcaatgc gatttatcag cgaacaggta cgcctattca tcctatgtcg ccattagcga
     7381 agattttttg gatgaaacat gaatggcaag atgtatttca acgaactgct aagtttgcag
     7441 atattaaaac atacattttc tatcatttat ttgatagata tatcattgat tattcaatgg
     7501 cttccgcaac agggatgttt aatttagaaa cattagattg ggatgttggg gcattagaat
     7561 tgcttggtat ttccgaggaa atgttgccgg aattagtgcc aacaacgtat gtgatgaaag
     7621 gtatgaaaga acgttatgca acattaatgg ggcttaatga agatacaccg tttgttattg
     7681 gtgcgagtga tggggtcctt tctaatttgg gtgtcaatag tgttggtaaa ggagaagttg
     7741 ctgtcacaat cggtacatct ggggcgattc gtactgtgat agataaacca cgtactgatt
     7801 acaaaggtag aatattttgt tatgtcttaa cagaggacca ctacgtcatt ggaggccctg
     7861 taaataatgg tggtgtcgta ttgagatggt tgcgcgatga gttgttagca agcgaagtcg
     7921 aaactgcgaa acgtctcggt gttgatcctt atgatgtttt aactcaaatt gcaaagcgtg
     7981 ttaaaccagg tgcagatggt ttaatattcc atccctattt agctggagaa cgtgcaccgc
     8041 tttggaacgc aaatgcaaga ggttcattct tcggtttaac tttatctcat aaaaaagaac
     8101 atatgattcg cgctgcatta gaaggtgttc tttacaattt gtataccgtc tatcttgcgc
     8161 ttattgaagt aatgaatgaa acacctaaga tgataaaagc aacaggtggt tttgcgaaaa
     8221 gtgaagtatg gcgtcaaatg atgtcagata tatttgacac agagttagtg gttcctgaaa
     8281 gttatgaaag ttcatgctta ggtgcctgcg tactcgggct taaagctgta ggtgacattg
     8341 aagatttttc aatcgtttca tcgatggtcg gtgccacaaa caatcatacg ccgattgaag
     8401 aaaatgtcgc tgtttatcaa gagctcgtat ccatttttat caatttaagt cgttctttaa
     8461 cagagaatta tgaacaaatt gcagattttc aacgcaaaca tatggctgaa aataaaacac
     8521 aataaatacg tcactcaagc atcttagata aagttgttgt gccatgacta ctatatatta
     8581 tgtggaatac gtgcgcgacg acggtgttac attctattga aaagagaaag aggggaatat
     8641 catgtttaac gaaatatggc cgttaatcag tgttgtttta ggtataatta ttttattagt
     8701 attaatcatt gggtttaaat taaatacatt tatttcatta attatcacat cgatgattac
     8761 agcgttaatg ctaggtatac cattgactaa aattatggag acgattgaga aagggatggg
     8821 cagtacgctc ggccacattg cactgatatt cggtttaggt gccatacttg ggaaattact
     8881 tgctgatggt ggtggtgcaa ctcgaattgc agacacgttg attcaaaaat ttggtcaaaa
     8941 acatgtacaa tgggcaatgc ttgttgcagc atttatcgtc gggattgcat tgttctttga
     9001 agtaggttta gtcttactga ttccattagt atttacagta gcaaaacgcg caaatgtttc
     9061 agtgttaaaa ctaggattac ctatggtaac agctttatca gtgacacatg gctttttacc
     9121 accacggtag tcatcgcaaa agaattaaag gcgaatgtag gagatgtatt actatacggt
     9181 atgattattg ccattccagt tacactcatt gcaggaccta tatttaacaa agttgcacaa
     9241 aaaatgattc cgtctgcgta tacacgagaa ggcgatattt cagcgttagg tgcacaaaaa
     9301 gaatttacgg atcaagagat gccaggattt ggtatgagtt tattaacagc aacattacca
     9361 gtcatattaa tgttagtgtc tacgataacg caacttgtaa ccgggcacga caaacctaca
     9421 aatctatttg aatctatcat ttatatgatc ggaacagcag ggacagccat gctaatagca
     9481 gtattatttg caatcgttac gatgggatta atgagaaaac gtaagatgaa tcatattatg
     9541 gaatcagtga cgaatgcgat ttatccaatc ggaatgatgt tattgattat tggcggtggc
     9601 ggtacattta aacaagtatt aattgacggt ggtgtcggaa atacaattgc taaaatgttt
     9661 gaaggtacag agatgtctcc gattttacta gcttggattg ttgcagctgt gctacgtatc
     9721 gcattaggtt cggctacagt agctgcgatt tcaactacag gtattgtctt accattatta
     9781 caatcatcag atgtaaatgt tgcattagtt gtactagcga ttggtgcagg tagtgtgatt
     9841 ttgtctcatg ttaatgacgc aggattctgg atgtttaaag aatattttgg tttaactgta
     9901 aaagaaacat tcctaacatg gtcattatta gaaacgatta tttccgtttc aggtatcatc
     9961 ttcattttat tcatcagctt atttgtttaa ctaaatatta agtaaacgtg tttttaggta
    10021 attacaatat attgcctcga aacacgttta tttttatgca gaaaatcgtc tgaaatactc
    10081 agaatccgaa cgatataaaa atatattggt tgttttagtg taatgaaagg cgtttcaaga
    10141 catgtttttg tgcgtattgt acaggcgata attatgaaac gtttagtatg ttgttttaaa
    10201 ttagataatg atgaatttaa tttaaaaaat aagtataaaa aatacaagcc ttgtgtgaca
    10261 agggtttctg acgacttgga tacaatttat aagtatattt caaataataa aattatcaat
    10321 taacataaaa ttaatgacaa tcttaacttt tcattaactc gcttttttgt attgctttta
    10381 aaaaccgaac aatatagact tgcatttatt aagtttaaaa aaattaatga attttgcatt
    10441 taaagggaga tattatagtg aaaaacaatc ttaggtacgg cattagaaaa cataaattgg
    10501 gagcagcatc agtattctta ggaacaatga tcgttgttgg gatgggacaa gataaagaag
    10561 ctgcagcatc agaacaaaag acaactacag tagaagaaaa tgggaattca gctactgata
    10621 ataaagtaag cgaaacacaa acaactacaa ctaacgttaa tactatagat gaaacacaat
    10681 catacagcgc aacagcaaca gaacaaccgt caaacgcaac acaagtaaca actgaagaag
    10741 caccaaaagc agtacaagcg ccacaaactg cacaaccagc aaatgtagaa acagttaaag
    10801 aagaggtagt taaggaagaa gcgaaccctc aagttaagga aacgacacaa tctcaagaca
    10861 atagcggaga tcaaagacaa gtagatttaa cacctaaaaa ggctacacaa aatcaagtag
    10921 cagaaacaca agttgaagtg gcacagccaa gaacggcatt agaaagtaag ccacgtgtga
    10981 caagatcaac agatgtagcg gaagctaagg aagctagtga cgcgaaagtg gaaacgggta
    11041 cagatgtgac aagtaaagtt acagtggaag atgaaagtaa aattgaagcg ccaaaaggaa
    11101 ataatgttca gcctcatgaa gggcaacgtg tagtattaaa gtataaactg aaatttcaag
    11161 atgggttgaa aacaggcgat tattttgatt ttacattatc aaataatgta aatacacatg
    11221 gtgtagcaac tacaagaaaa gttccagata ttaaaaatgg atcattagtt atggctaaag
    11281 gtcaggtatt agataatggc cgaattagat atacatttac tgattacatc aaagataaag
    11341 taaatgttac agctaattta gaaataaact tattcataga tcctaaaact gtacaaagca
    11401 atggacaaca aacaataact tcaaaattaa atggcaaaga aacatcagga actatgcaaa
    11461 taacatataa agatggtgtt aaaaatcaat atacaaatgt aaatggttcg attgaaacgt
    11521 ttgacaaaga aaaaaataaa tttacacacg tagcatatat taagcctata aatggaaaca
    11581 attcagatag tgttactgta acgggtatgt tgacacaagg aagtaatgag aatggaacac
    11641 aaccaaatgt taaaatatat gagtatgttg gagttgaaaa tgggctacca caaagtgttt
    11701 atgcaaatac agtggacagc acacaattga aagatgtaac gaatcaaatg ggtgataaat
    11761 tgaaagtaca aaacaatggt agctactcat taaattttga caaattagat aaaacatatg
    11821 taattcatta caccggagat tacttaaatg gtacgagtga agtgaacttt agaactcaat
    11881 taactggata ccctgaaaat cgctataaaa catattatta ttataacaat ggctatacat
    11941 taacatggga taatggatta gttttatata gtaataaagc taatggtgat ggaaaatatg
    12001 gtccaatcgt agactccaat aactttgagt tctctgaaga tagtggtaat ggttcaatca
    12061 gcggacaata cgatgcgaag caaattattg aaacagaaga aaatcaagac aatacaccgc
    12121 ttgacattga ttaccacaca gctatagatg gtgaaggtgg ttatgttgat ggatacattg
    12181 aaacaataga agaaacggat tcatcagcta ttgatatcga ttaccatact gctgtggata
    12241 gcgaagcgcg tcacgttgga ggatacactg agtcctctga ggaatcaaat ccaattgact
    12301 ttgaagaatc tacacatgaa aattcaaaac atcacgctga tgttgttgaa tatgaagaag
    12361 atacaaaccc aggtggcggt caggttacta ctgaatctaa cttagttgaa tttgacgaag
    12421 agtctacaaa aggtattgta actggcgcag tgagcgacca tacaacagtt gaagatacga
    12481 aagaatatac aactgaaagt aatctgattg aactagtaga tgaactacct gaagaacatg
    12541 gtcaagcaca agggccagtc gaggaaatca ctgaaaacaa tcatcatatt tctcattctg
    12601 gtttaggaac tgaaaatggt cacggtaatt atggcgtgat tgaagaaatc gaagaaaata
    12661 gccatgttga tattaagagt gaattaggtt acgaaggtgg ccaaaatagc ggtaatcagt
    12721 cattcgagga agacacagaa gaagacaaac ctaagtatga acaaggtggt aacatcattg
    12781 atatcgactt cgacagtgtg ccacaaattc atggattcaa taagcataat gaaattattg
    12841 aagaagatac aaacaaagat aaacctaatt atcaattcgg tggacacaac agtgttgatt
    12901 ttgaagaaga tacacttcca aaagtaagcg gtcaaaacga aggtcaacaa acgattgaag
    12961 aagatacaac gccgccaaca ccaccgacac cagaggtacc aagtgagcca ggcacaccaa
    13021 caccgccaac gccagaagtg ccaagtgagc caggcacacc aacaccacca acaccagagg
    13081 taccagctga acctggtaaa ccagtaccac ctgctaaaga agaacctaaa aagccttcta
    13141 aaccagtgga acaaggtaaa gtagtaacac ctgttattga aattaatgaa aaggttaaag
    13201 cagtggcacc aactaaacaa aaacaagcta agaaatctga actacctgaa acaggtggag
    13261 aagaatcaac aaacaaaggt atgttgttcg gcggattatt cagcattcta ggtttagcgt
    13321 tattacgcag aaataaaaag aatcacaaag cataatcaat ctaaaattga caggtttatt
    13381 tcataaatta tatgaagtaa gcctgttttt taaaattaaa tcaaatttcc caagaaataa
    13441 ttacatactc aatgacacta tgaaggcgtt ctaattagtg ttaaaatgac gttgatacat
    13501 agatttaata cttaggaaaa ggagcacatt aactttgaaa aaaataaaaa aggcaatcat
    13561 tcccgctgct ggtttaggga ctagattttt accagcaact aaagcgatgc caaaggaaat
    13621 gcttcctatc ttagataaac cgacaataca atatatcatt gaagaagctg caagagctgg
    13681 aattgaagat attattatag tgacaggtcg ccacaaacgc gcgattgaag atcattttga
    13741 tagtcaaaaa gaattagaaa tggtgttaaa agaaaaaggt aaatctgaat tactagaaaa
    13801 agttcagtat tcaacggaac ttgcgaatat tttttatgta aggcagaaag aacaaaaagg
    13861 tttagggcat gcgattagtt ctgcgcgtca atttatcggc aatgaaccat ttgcggtatt
    13921 attaggtgac gatattgttg aatctgaagt gccagcagtg aaacagttga ttgacgttta
    13981 tgaagaaacg gggcactcag ttataggtgt tcaagaagtt ccggaagcag atactcatcg
    14041 ttatggaatt attgatccat taactaaaaa tggacgtcag tatgaagtga aaaagtttgt
    14101 tgaaaagcca gcacaaggta cggcaccatc aaacctagct attatggggc gatatgtatt
    14161 aacgcctgaa attttcgatt atttaaaaac gcaaaaagaa ggcgcaggta acgaaattca
    14221 gttaacagat gcgattgagc gtatgaataa tgataaccaa gtgtatgcat atgacttcga
    14281 aggcgaacgt tatgacgttg gagaaaaatt aggcttcgtt aaaactacca tagaatatgc
    14341 attgaaagat gacagtatgc gagaagaatt aacacgattt attaaagaat taggtttata
    14401 acattaaaaa gcataaatga ttctatatta atgcaacttt gtattcgtac aaggttgtat
    14461 ttttttcgca aacaattgct tgctgaaagc gcaccattaa atctaaccaa cacactatca
    14521 caaacattta tggatatttt gtaattttat aaaagcaaac gtagaataat gcgataagta
    14581 ataatgctat cgttaaaaat aagaatgaca atgcaataaa atcatattcg aagcatagaa
    14641 ttgcagacat taaaaatact gcactcaaac tgatacatag cgcaaatata tatgcaattt
    14701 gggtcttgtg tttatctgca atggtcgttt gatttataaa agagattagt gtgaaatctt
    14761 tagttattgc gacataaatg cctaatataa ccatactgat acagaacacg atagaaaata
    14821 cgatgttgac ggtcattgtt gcaccttccc gatagaatat aatgtactgt cttttagtga
    14881 ttgatgcgtt tcttatatat gtatgtttct aaaaaacagt acgtttttat caatttttaa
    14941 tttattagtt gaatgaatgg ggttatgaaa aaggaagttt taatcttttg tcatgaaata
    15001 aatgggatga atatcacgac tagaagtaat gttacgaaca ggagcgtata aatctagaga
    15061 cgctaaattc gacatagtat gttgctcaat tatggctgat gatgaattta aagtatgtgc
    15121 gttggaactg tcggattttt gttcataatg ttttgcatat tgtgccatga tgaatagtgt
    15181 aaaaataaat aaaacaataa gagatataat gcccataatc aaaagtattt gtttagagcc
    15241 tttcattatt tcacatcctt tctaaaatat atttgtaact aaatttaaaa tagttatttt
    15301 tgtaattcta aacctttttc atcgcgaaaa caattaaata ggtcgcggta ttaattatta
    15361 tattattacc gcttaatatg aaaaatacat gaaaattaat tttctaatat acttttgaaa
    15421 aattattaca aattagcccc ttcaaaacgc gaaaacataa ggattctagt ttcaaaaggg
    15481 ctgataagta taaaatgaaa tgtaatattt cgatgtttaa aatttttaat tagctaaaaa
    15541 tcatcgcatt aattttttga gctacatcat caaaatccgg acattttaac gacacatata
    15601 atttaatttt aggttcagta ccagaaggac gtaaagcgat aaatccttcg tcaaataaga
    15661 cacgaataac atttgactta ggagaatcaa tctgcgacgt tgtatcttta tccaactgat
    15721 aaacctcgct agttaaataa tcttcaattg ctttcacttt cagtccttga atctcttgcg
    15781 gtggatttga acggaaatgt gtcataattg attcaatttt tttcttacct tctaaacctt
    15841 ctagcgtatg cgagaataat gtatcctcat gtctaccaac agtttgataa atctgttcta
    15901 attcatcttt caatgttttg ccatataatt ttaactcaga agcgtatttt ataatgagtg
    15961 gcacaatttg tacggcatct ttatcacgta caaaaggctc tgatagaaaa ccgtaactct
    16021 cttcaaatgc gaaaatcata ttttgatgat cgtccagttg tcttatttcc tgagcaataa
    16081 atttaaagcc cgtcagcacc tctttgtatt caacattatt atagcgtgct aatgactttg
    16141 ttaactcact acttacaata gattgaatca ttaagcggtg acgtaattga gacgtttgtt
    16201 gaatacgata attaagtaac agcgcaccaa tttggttgcc attaaaatat gtgatatgac
    16261 cgtgtgcatc acgttcagca ataccaagac gatccgcatc agggtcagtt gatattaata
    16321 agtctgcatg acttttattt gcaagttcga ctgcttgatc aaaagcgcga tgatcttcag
    16381 gattagcact ttgaactgag ctaaaattcg gatcaggttt acattgtgct tctacaagat
    16441 tgaattgatt gaaattcaaa gattttaata actcaggtac aatcggaaca cttgtaccat
    16501 gcaaactagt gaacacaact tgtaaatcag atttaggaat atagccaatc atattttgaa
    16561 tgtgtttcat atagtcatca gttacagatt tcgggaaagg cttgatataa gatgtatttt
    16621 gttttgaaat aggtatatca atctgtaatg gatcgccaac ttcttcgata taacgactta
    16681 caagctcaga tgcatcggtc gatagttgcg cgccatcaga actatatact ttaatgccgt
    16741 tatagtcttt cggattatga cttgctgtaa tcataatgcc agcagtagta ttaagattgc
    16801 gcaccgcgaa cgataattcc ggtgtcgttt tatatgtatc aggtaaataa actattattt
    16861 gatggtttgc taatacatta gcaatgattt gggcgaattc agttgaaaga tgtctaatat
    16921 cataatgaat gactattgtt ggattgtttg tttgggcatt taaataacgc gctaaaccta
    16981 atgccaattt ttcaatagta aacttattaa gtcgaccttc accaagaccg aattttccgc
    17041 gtatacccgc agtaccaaac gttaatttac tttcaaaacc ttctcgctgt tcaatgtcag
    17101 attgttgctc ataaaaatgt ttaactaaac tatcattagc tctttttatc cataactctt
    17161 tatccattgt tgctaaacat cctttcaaaa tctcagttag acttaataaa acatgaaaac
    17221 taaagccctt acatttatgt aatgaattat aaagaaatac gccccaaaag taaaaaaaca
    17281 cagccccaag acaatacttt tcacaagtat tatataatag atgtgtatga aaatgcatgg
    17341 agtagagcgt tcatgtgaat gtaaaatatt
//
LOCUS       DATTQF010000045        17257 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.045, whole genome shotgun sequence.
ACCESSION   DATTQF010000045 DATTQF010000000
VERSION     DATTQF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.045"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1737)
                     /locus_tag="VB241_001270"
     CDS             complement(<1..1737)
                     /locus_tag="VB241_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1244571.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFC"
     gene            complement(1734..2819)
                     /locus_tag="VB241_001271"
     CDS             complement(1734..2819)
                     /locus_tag="VB241_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1244572.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            complement(2953..3492)
                     /gene="radC"
                     /locus_tag="VB241_001272"
                     /pseudo
     CDS             complement(2953..3492)
                     /gene="radC"
                     /locus_tag="VB241_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(3489..4196)
                     /locus_tag="VB241_001273"
     CDS             complement(3489..4196)
                     /locus_tag="VB241_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP1244573.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCHKRISLTHFFGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSITYPVAFIIISMTTHIFYFLFRSYIG
                     YGDVLLISALSLFFPLQFTIYVIIFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(4458..5729)
                     /locus_tag="VB241_001274"
     CDS             complement(4458..5729)
                     /locus_tag="VB241_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP1244574.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPI
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDDIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(5742..8372)
                     /locus_tag="VB241_001275"
     CDS             complement(5742..8372)
                     /locus_tag="VB241_001275"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP1244575.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNNKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWIENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGETIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(8416..8717)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8775..9335
                     /locus_tag="VB241_001276"
     CDS             8775..9335
                     /locus_tag="VB241_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP1244576.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAHDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAFGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPRHN"
     gene            complement(9558..10631)
                     /locus_tag="VB241_001277"
     CDS             complement(9558..10631)
                     /locus_tag="VB241_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEP1244577.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(10665..10778)
                     /locus_tag="VB241_001278"
     CDS             complement(10665..10778)
                     /locus_tag="VB241_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244578.1"
                     /translation="MYIRHGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(10826..12112)
                     /gene="hemL"
                     /locus_tag="VB241_001279"
     CDS             complement(10826..12112)
                     /gene="hemL"
                     /locus_tag="VB241_001279"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEP1244579.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(12160..13134)
                     /gene="hemB"
                     /locus_tag="VB241_001280"
     CDS             complement(12160..13134)
                     /gene="hemB"
                     /locus_tag="VB241_001280"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEP1244580.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(13137..13805)
                     /locus_tag="VB241_001281"
     CDS             complement(13137..13805)
                     /locus_tag="VB241_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEP1244581.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLESF
                     NQTNQKILLPSSELARPLLSAALSEDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(13827..14753)
                     /gene="hemC"
                     /locus_tag="VB241_001282"
     CDS             complement(13827..14753)
                     /gene="hemC"
                     /locus_tag="VB241_001282"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEP1244582.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKKVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(14795..15610)
                     /gene="ccsA"
                     /locus_tag="VB241_001283"
     CDS             complement(14795..15610)
                     /gene="ccsA"
                     /locus_tag="VB241_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEP1244583.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTKHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(15632..16978)
                     /locus_tag="VB241_001284"
     CDS             complement(15632..16978)
                     /locus_tag="VB241_001284"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEP1244584.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WVSMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAKCPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(17194..>17257)
                     /locus_tag="VB241_001285"
     CDS             complement(17194..>17257)
                     /locus_tag="VB241_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP1244585.1"
                     /translation="SSIQNNKQQQIWNLIEPYIS"
BASE COUNT         5586 a         3284 c         2324 g         6063 t
ORIGIN      
        1 acagaagtga gtgcaatcta agcatgcatt cgcatgtgga caaccacctg ctactactgg
       61 caatctacaa taaccatttg gaagcacttg tgcatttata tttttcttga accattgaag
      121 ctctacatca tcgacttcat tatcttcatc tagatcaagc acatctccat tattggtaac
      181 cagtttttcc tgaaatttag taaattcatt ttttagagtt tcatcaaaga tatgagcgta
      241 tctgcttgtc atttctgggc tttcatgccc caaaaatttc tgcacaatat gctggggcat
      301 cccgttgtta atcattcttg ttcctactgt atggcgaaag gcatgggcat ggaatctata
      361 aatctcacct gatttatcca ctatattttg ctcataagct aatttattta actcacctct
      421 aaatgtttct tgttttaatg gcgatccatc ttttcttgga aagaggtatt cactatctgg
      481 aaattcctct gaaactttat cttcccgaac tttaataagt aaagctacct ctttagatat
      541 tggaactata tgctcctttt tcattttcca ttgataatac tttaaaaaga aatctccatc
      601 tttgtcctct aatagacagc cttttttcaa ggtgcacaat tcacttatcc tcattccaca
      661 ttcttgaaca atcatagtca tcgtagctat atattcgggt aatttatcaa gatgactgtt
      721 caattgctct aggacgaatt catctataaa gcgtggtttt gctcttggta ttttcggata
      781 gtcctcagaa taaattaata ttttggaagg aacatcatcc cattctagcc taagaagggt
      841 actaaatagt ccttccaata tagagatcct cccagttatt gtactaggtt ttattcccat
      901 catgtttagt tcacttaaat atgcttcaat ttccactctc gttaattggt gtactctctg
      961 aacttgttta aatttcatgt ccagaaaatt aaagaactct ttaagtcttt gggcaatatc
     1021 acttacatag gaaaagctat ccacgttcaa tctcaactta caatatcttt ttacaagttg
     1081 tttaaaatat gtattccgaa accctttaaa gttaattgta tattcatatt gtgttgggtt
     1141 aaccttatca tctggcaaag gtaagttacg tctatcccaa acgtctttat cccactcctc
     1201 tccatcaaaa taaaagttct cataaaactc cataaattgt tttagattag taacatagta
     1261 ggaattagct tttacaggtg ttttttcttg attagcagta atcttataat tagtagtggt
     1321 aattctaaca ccccgttttg tcaaataagt tctatactcc gtcattgctt tttcaatagg
     1381 aacttcagta attgaagtaa tgctaggata ctttaaatct aagaaatcta acattttatt
     1441 aattactgtt ccttttctaa tccagacagt ttttgcattc catattccat tgtttaaatg
     1501 gtaaaaataa aaatatttca attctgttct taaccacaga tttttaacac gttcaaaacg
     1561 aacccaacga ttccttaaag caggattctt acttaattct atggcagaag gatgtggaca
     1621 ttttcttata tcccaactat tattagccca aaacccctgc atttcttcat tcattacagc
     1681 tatttttttg ctaatctcac tctgactaat aattttcctt ttactagaag cattcatttc
     1741 ttatgctcct ttctctcgag gtatttatta aactcatttt tcatatcctg atctgaaaga
     1801 tgaacatagg tatttaacgt tgtctgaaca tgtgcgtgac ctaatctctt ttgaacgaac
     1861 gcaacatccc atccttccct aattagctgc gttgcgtgag tgtggcgaag catatgtgat
     1921 gtaaattcta ttccagtcct tttaactatt cttctaacta gatcaagaac actttggtac
     1981 tttagtggtt tcccaaaata gccttctttt aaggaaataa aaacataatc atgctccaat
     2041 tcctcactat actcatatat caagtaatct gtataaagtg acataagttc tttactcaca
     2101 tgtattgttc tttccttcct taatttaata taagcttcat taacattaac atctctaggt
     2161 gttaaatgga tttgattgtc ccaagtgaca atatcttcaa gcctaagcga taacacttca
     2221 ccgattctta aaccaccctc atacataagc attaaaatta atttatctct tttcgtatga
     2281 caagcatcaa taatttgctt aacttccttt gatctcaatg ttcttatctg tttcttttta
     2341 acccttaact ttaagacatt cttttggtat ctacccttat taacatgatg taaaaatcct
     2401 ttgaaatttc ttccctttgc ttgtttaaat acatcaattg atttaaattc tcctaatcta
     2461 cttaaataat caagaaaact cataactaca tttaaaattg tattcactgt cgtttcttct
     2521 cttatggctt tttttgactg aagatcaatt acatttgatg ctgaaggata tctcaaccaa
     2581 cctacgaagt ctgctaacaa ctcaaagtta atatcattaa gaataacacc tctctgttcc
     2641 atgaactcgt acagcaactt taaatgatag cagtatgcct taatggtatt aggagactta
     2701 ccagtattat ctaagtattt aataaatttc attactggtt ctattagctg gtattcttta
     2761 tctagtaata aatacaatgg atacggctta ttctccactt ctatcctttg aaccttcaca
     2821 tgttcccacc tctttaaata ctttaactac tacaaattat aaaccttgtt aatttttatt
     2881 taaagtaaaa tatcccttta atatctcctc ttaaactact ttaactacta ttatttatta
     2941 tactatggtt aatacatcac cggatggatg attatgaact gcgatgattg cattggcatt
     3001 ttctctcacc gcaatactaa aaaattcacg tggatgtaca atcgaactat ttaatgtacc
     3061 tttaaaaaca caggtttctt taatcactac attttttgaa tttaacaata aaatgacaaa
     3121 atgttcttgt gttaaatctt tcattgttgg aatcatataa tcagcaacat cacttggttg
     3181 cgttattttt atacgattat tttcagctct tctccccatc ctttccccta actcaaatgc
     3241 cgcttttaat gtaattgctt tttgtaatcc aatcccttta acttgtatca aatcgttaat
     3301 tgaagatttt ttcaattcat tcagattcga tgcagattta agcagttcat tactaatgtc
     3361 aatactcgag aatccttttc ttccggtgtt aattaatata gctaataatt ctgtattcga
     3421 aagacttttt gcaccatggc ttaacaaacg ttctcttggc atttctgaag ttaccatttc
     3481 tttaattttc aaaaatatac gcctcctaaa aattgatgga tatcattata aaaaagtgaa
     3541 ttgataaaaa aggaaataaa tataaatgga acaaggggta atagtttaat cggcttaaat
     3601 atcatggtaa ttaaagcaac taaaccagca atgacaaatg taaatataat gacataaata
     3661 gtgaattgga gagggaaaaa caaagaaagt gcagatatta gtaaaacgtc accataacca
     3721 atatatgacc gaaataaaaa gtagaatata tgcgtggtca tactaataat gataaaagct
     3781 actggataag taatacttaa cgagagagaa acaatacaat aaattataat taagcgacaa
     3841 tctaacatta aagaagtgat atcggtcata gtaaaaataa gcagaaaaac atatgtagtt
     3901 ataaaaagcg tagcatttac gtatgtgaaa tcatacttaa taaagacgat aggtattaaa
     3961 gcaaaggttt ccccaaagaa atgtgttagc gaaatacgct tatgacagtt tcgacatctc
     4021 ccttttaata ataaaaaact aataatcggc attaattcat accatttgag tgatgaatta
     4081 caataatcac atttcgatct tctatgtaaa taatcaaatg acgtttcttc aatagatata
     4141 aattgatata gaaaactaaa aatacaactg caactataag ataacaatac taccaaatga
     4201 caacctcctt acgtaaatta tagttactta ttaccaaaat gtaaatatac actattctcc
     4261 aaatattgaa cctctttttc atttaaattt ttcaatatta ctaagcataa ttgatggata
     4321 ctttaacagc ccattactgc tcggcaaatt taataatggc aagaaattaa ccttataaac
     4381 acatacgatt tagagcataa aaaaataacc atgaagctac ctattgatta aatagattct
     4441 tcatggctat tttagtttta taatgcttca aagtctaatt ttgatttaac ttcacttatg
     4501 aagtacagac taccggtaat tactaatgta tcaccttgat aattttttat aaattctacg
     4561 tagtcatcta ctaattgtat atcgtcattt tcaatgctac ctacaatttc ttctttgcgt
     4621 aacgctttcg gaaaatcaaa ttcagttgca taaaatgtat gcgcaattaa acttaaatgt
     4681 ttgaccatct cgttaatcgg ttttccgttt attgctgaga acaaaatatc tactttttct
     4741 ttatcatggt actgtttaat tgtatcaatt agagcatcta tactctctga attatgtgcg
     4801 ccatccaaaa tgattaaagg tttgtcatgc acctgctcaa tacgtccagt ccaacgaact
     4861 gattcaatac cgtctatcat cttattgaaa tctaattcaa ttaatccttg ttcatttaat
     4921 tcaataagag ctgttatggc taatgcagca ttttgtttct gatgttcacc taacatactt
     4981 aaaatgattg tttctaattc ataatcttta taacggtaag taaattcatc attttgcgat
     5041 acaacaacaa tttctctatc taattcaatt ggctttgcat gttgttcaat tgcgcgttca
     5101 cgaacatact ttaatgcatc ttcattttta acagcatata taactggaac gttaggcttt
     5161 ataatcgcgc ctttatccct agcaatatct agataagtac cacctaaaat atctgtatgg
     5221 tctagaccga tactagttaa gattgataaa atcggtgtaa agacatttgt cgaatcgttc
     5281 tttataccca atccagcctc aacaatgaca aaatcaacag gatgtatttc accaaaatat
     5341 aaaaacatca tcgctgtgat tatttcgaat tcagttgcaa cacctaaatc tgtttcacgt
     5401 tccatcattt cactaactgg tttaatacgt gatactaatt ctacaatagc gtcatttgat
     5461 attggcacac catttagact aattcgttca ttaaatgttt caataaacgg cgacgtaaat
     5521 gtacctactt cataaccatt ttcaactaaa gctgttctaa ggtaagcaac tgtagagcct
     5581 ttaccattcg tgccacctac atgaataccc ttaatgttat tttgaggatt attaaattgt
     5641 gctagcatcc attccatacg tttaacacct ggtttgatgc caaatttagt tctttcgtgt
     5701 atccaataca agctctctag gtaattcatt gttactaact cctatgcttt taattgttca
     5761 attcttgcct tcacaccatc atatttttct tgataatctt gttttttacg tttttcttca
     5821 tttataacct tttcaggtgc tttacttaca aagttttcat tagagagctt tttatctact
     5881 ctatctaatt cgctttgaag tttagctaat tctttttcca aacggctgat ttccttatcc
     5941 atatcaatta gcccttctaa tggtaatacc actttacctg caattacaac tgatgtcatt
     6001 gctttctcag gaatttccac gtcagtgctt atatttaacg tactaggatt acagaatttg
     6061 attaaataat ctttgttttg tgataaagtt gtttcaattt ctttatcttt agcttgaatt
     6121 aaaataggta tttctttaga caatggcgta tttacttcta cacgtgattg tcttacagat
     6181 ttaatgattt caacaagttg ttgcattgtt tgtttacttt cttcaaaaat caatgattca
     6241 cgcacttctg gccatgaagc tttaacaatt gtttcacctt catgtggtaa actttgccat
     6301 attttctctg ttacaaatgg catgaatgga tgtagcattc tcataatatt gtctaaagta
     6361 taactcaata ctgaacgtgt aacttgtttt tgttcttcat cattaccatt cattggaatt
     6421 ttactcattt caatgtacca atcacagaaa tcatcccaaa tgaaattata taatgctcgt
     6481 ccaacttcgc cgaattcata tttgtcactt agatcagtaa ctgttgcaat cgtttcattt
     6541 aaacgtgtta gaatccattt atctgctaat gataagttac cacttaaatc gatatcttca
     6601 actttaaagt cttcaccgat attcattaaa ctgaaacgtg ctccattcca aattttattg
     6661 ataaagttcc acactgactc aactttttca gttgagtatc ttaaatcatg tcctggagat
     6721 gaacccgttg ctaagaagta acgcaagcta tcagcaccgt attcgtcaat aacatccatt
     6781 ggatccacac cattacctaa tgatttactc atcttacgcc cgtcttcagc gcgaactaag
     6841 ccgtgtaata acacatcatt aaatggacga cgatctgtaa attctaagcc ttggaatatc
     6901 atacgtgcta cccagaaaaa gataatgtcg taacctgtaa ctaaggcatt tgttgggtag
     6961 tatcgtttaa agtcttcgct ttctaaatca ggccaaccta acgtagagaa aggccataaa
     7021 gcacttgaga accacgtatc taatacatct tcatcttgtt gccaattttc aatatcagtt
     7081 ggcgcttctt ctccaacata tatttctcct gtttctttat gataccaagc cggaatttga
     7141 tgaccccacc ataattgtct tgaaatcgtc caatctctaa tattttcaat ccattggtta
     7201 aatgtatgtt cgaaacgttg cggataaaaa tcaatacgat catctgtttt ttggttatct
     7261 aatgaacgtt tcgctaagtc ttccatgcgc acaaaccatt gcgttgataa atatggttca
     7321 acaacagcgc cagatcgttc tgaatgacct acagaatgaa catgatcttc aatcttgata
     7381 actaaatctt gttcttttaa atcttcaact agctgtttac gacaatcaaa acggtccata
     7441 ccttcgtatt taccagcttt gttgttcatt ttaccatttt catccataac gataatattt
     7501 tctaattgat gtctttgacc aatttcaaaa tcattagggt catgtgctgg tgtcactttc
     7561 atagcaccag aaccgaagtc tatatcaaca tattcatctg ctaaaatagg cagttcgcgt
     7621 cctacgattg gtaatataac agttttaccg attacatctt tgtatcgttc gtcattaggg
     7681 ttaacaacaa tcgctgtatc acctaacatc gtttctggtc ttgtagttgc aatttcaata
     7741 aaaccttcac catcagcgta aggatattta aaatgataaa acgcaccttg aacatcttca
     7801 tgtattactt caatatcaga taaagctgta cgtgctttag gatcccaatt tataatacgt
     7861 tcgccacgat aaataattcc tttattgtat aaatcaacaa aaactttttt aactgcttta
     7921 cttaaacctt catctaaagt aaaacgttct ctactataat ctaaacctag acctaattta
     7981 gcccattgcg cacgaataaa tgacgcatac tcttctttcc aatcccatgc ttgttctaaa
     8041 aacttttcac gaccaagatc atatctagtt attccttgtt catttaattt agcttctacc
     8101 tttgcctgtg tcgcaatacc agcatgatcc atacctggta agtataacgt atcgtatcct
     8161 tgcatacgtt tcatacgtgt aatgatatct tgtaaagttg tatcccatgc atgtcctaaa
     8221 tgtaatttac cagttacatt tggtggcggg ataacaattg tatatgtttc ttttgattta
     8281 tcttctgacg gtttaaaata accattcttc acccattctt cataacgtcc cgcttcaact
     8341 tcacgaggat catattttgg tttcatttcc atttgctata cctcctaaaa aataaaaaat
     8401 atccatccta tatacaaata ggacggatat tccgtggtac cacctatatt caagaaggat
     8461 gattaatatc aaattcactc ttttaacata attggaataa tcataccaat actatcatcg
     8521 tgaaatttga aatgcttcat ctcttcaagc actctagatt atgattaacg ctcaaacacg
     8581 tcttagccta ctattaatca cgttcagcta agatactctg tgggctacct tcagtaaaaa
     8641 tcatttacat actcacacca aatcatatgc tctctttaaa ataatttgaa cttactcttc
     8701 ccaaatccta tattaaactc ttaacttata gtataatgat tgacaaaata agtcaatgta
     8761 taggtgggaa taaaatgaat gaatgcgcat ttggaactaa agatccagtc tacttagact
     8821 atcatgatca tgtatgggga caaccgctct atgatagcaa ggcattgttt aaacttttag
     8881 cattagaatc acaacatgct gggctatctt ggttaactat tttaaaaaag aaagaagcct
     8941 atgaagaagc attttatgat ttcgaaccag aaaaggtagc acaaatgacc gctcatgata
     9001 tcgaccgctt aatgactttt ccaaatatcg ttcatcatcg taaaaaatta gaagcaattg
     9061 ttaatcaagc tcaagggtat ttaaaaattg aacaagcatt tggtagtttt agtaaatttt
     9121 tatggtctta tgtaaatggt aagcctaaag atttgcagta tgaacatgct tctgatcgta
     9181 tcacagttga tgatactgca acacaactat ctaaagattt aaaacaatac gggttcaaat
     9241 ttttaggtcc agtaacagta ttttcgtttt tagaggcagc cggtttatat gatgcacatt
     9301 taaaagattg tccatcaaag cctagacaca attaatttgc tatagcatca taaagtaaac
     9361 gagacatcat ctatttttaa ctaatagact gtctcgtttt ttaatgtaga aatttattca
     9421 aatcacaagt taaagttcca tgttaatgca cttctaatat tagatatctt aatttaaaaa
     9481 tagatgtaaa acctttgctt aaaccttaat aatgaattct atcctaagca taatttaact
     9541 taaatggatg atagtttcta atcttcacgc ttattcgaac gatattttaa aaagtaatta
     9601 attcctggtg caataataaa taggatgaaa aagattcgga aaatatgata actcgtaatc
     9661 atagcaacat cggcaccagt agctaatgca actaaaacta tctgattaac ccctcctggt
     9721 gctgcaccaa gaaacaattc attaatagga ttattatcaa agaaatgtat gatataaacc
     9781 atgattagcg caccaattat caacataata ttttgaattg taattgcgat tgctagtcta
     9841 ccttttaaat ctgacaataa atgcgcaatt tgaactccaa ttctaatcat atatattagt
     9901 tgtgccatgt tcaacaacca atgatctagt gtaaatgtta gacctgtaga aaaattccaa
     9961 acaattaata caatgagtgg tgctaataat tgaaatgttg gaaactttat tttagacata
    10021 attagataaa ctataaagat agctatcgct aaaataacta tttgccctat gtttaatact
    10081 tgtgataaag gcaagacttt tgttaactta ccattcgcat gcatgttacc atcatgaaaa
    10141 aaatatgaaa tgaacggtac taaaacaaca acaaatataa ttcgtgatgt ttgcgttaag
    10201 ctaacaacta acaaattagc acgtttgtct tgttcagcca tgaccaacat ttgtgttagt
    10261 gctcctggta taacacttaa aatagctgtt tctgtattaa tacgtgcaat ttttttaaaa
    10321 ataaatgcca ttactaaggc aattaataat atcgaaatag atactacaat aatcgaaagc
    10381 caattgtctt taatatccat aacgacattt ttcgtaaacg ttgatccgat ttgcacacct
    10441 aatagtacaa ttcctaattc actaagtaag aatggccatt taatatcaag tttgaaaact
    10501 tttacacaaa tgattgatgc gataatagga ccaaacataa atgggagtaa tacgtgcgat
    10561 gaatacagta gaatactaat aaaaaatgat aaaacgaaca caatgaaatt atttctatat
    10621 atcattgtca tgttttccac ttctttcaat aaaaaataaa atgactaaat tgctgcttga
    10681 gcttcacgtt tgttaagata acaatatccg ctagcagttt tgactacaaa gcatatatgg
    10741 actttcacta tcaagtcgcc gccatgcctg atatacattt aaaaagagcc tgaacaaagt
    10801 tcaggctctc aatttgtccg tatatttatt ttacaatacg acttaaagcc gtatcaaatg
    10861 cttgaatcgt tttttcaata tcttctttcg tgtgtgccgt agataagaat gtaccttcaa
    10921 attgagatgg tggtaaaaac acaccttctt ttgccatttc acgatacatt tctgcaaata
    10981 atttcaaatc acttttattc gcttgttcaa aattagttac aggtccttca tttaagaaat
    11041 aaccaatcat tgaacctgct ctatttacag ttattggtac attgtgttta gcaaatacac
    11101 gctttaaacc gtcttcaagt atatcgccta acatattaaa atactcatat gtctctggcg
    11161 ttaattgact taacgtttca taaccacttg tcattgcaag aggatttcct gataacgtac
    11221 cagcttgata aatatttcct aatggtgcta tatgatccat gatttctttt ttaccaccaa
    11281 aagcacctac tggtagtcct ccaccgataa cttttcctaa gcaagttaaa tctggtgtca
    11341 caccaaagtg accttgtgca caatgataac cgactctgaa accagtcatt acttcatcga
    11401 aaattagcaa tgcgccgtat tcagtcgtaa tatctcttaa tccctgtaaa aaaccttcaa
    11461 tcggcggtac gacacccata ttaccagcaa caggttctac gattacacca gcaatatcgt
    11521 ctccaaattt ttcgaaagcg attttaagtg catctaaatc attgtatgga actgtaattg
    11581 tatttttagc aataccttca ggcacaccag gagaatccgg caatcctaat gttgccaccc
    11641 ctgacccagc tttgattaat aacgaatcac tatgaccatg atagcaacct tcaaatttta
    11701 caattttatt tctgccagta taaccacgtg ctaatcttaa agtatccaat gtagcttctg
    11761 taccagatga caccatacgc actttttcta ttgaaggtac tcggtcaata acgagctgcg
    11821 ccaatttatt ttcaagtaat gttgatgcac caaaacttgt acctttatca attgcttcat
    11881 gtaaatgact aataacttga gggtctctat gtcctaaaat aagtggcccc caacttagta
    11941 catagtcgat atactcgtta ccatcgatat cataaatttt tgagccttta ccgtgatcca
    12001 taaaaattgc tggtgtatct actgatttaa atgcgcgtac tggactattt acaccaccag
    12061 gcattaaagt ttcagcaacc ttcattgctt cttctgactt cgtatatctc ataaagtaaa
    12121 ttcctcctat agtttatgga aaatcataca tataaaacct tatttatcta aatagcgaca
    12181 aatgtccttt gcaaaatacg taataatcat atcagcacct gcacgtttca ttgaaaccat
    12241 ttgttccata acgacacgtt cttcatcaat ccaaccattt tgtgccgctg ctttagtcat
    12301 actatattct ccactcacat tatatgcaac aactggaaca ttcgtatgat ttttaacgtc
    12361 tcgaactata tctaaataac ttagagcagg tttaacaatc atcatgtcgc acccttcttt
    12421 aagatcactt tctaattcac gaagtgcttc caaacggtta gcagggtcca tctgatacgt
    12481 ttttctatcc ccaaatgatg gcgctgaatc tgctgcatct ctaaaaggtc cgaagaaact
    12541 tgatgcatac ttgacaccat aactcattat aggaatattg taatagccgg cttcatctaa
    12601 tccacgacga atttcagcaa caaaaccatc catcatatta cttggtgcaa taatatcagc
    12661 accagcttct acttgagaaa ttgctgtttt aacaagtagt ggcaatgatt tatcattgtc
    12721 aacgtcatgt gtatggtcat caatcacacc acaatgacca tgatcagtat attcacataa
    12781 acaagtgtct gcaacaatta ataagtcatc atacattttt ttagcaatac gtgttgcctg
    12841 ttgaataaca ccatcgtgaa tgtatgcacc agtacctata tcatcttttg agtttggaac
    12901 accgaaaaac ataatggcac gtatgcctaa gtcataagct tcttttaatt cactttcaag
    12961 taaattcaaa ctgatttggt atacacctgg caatgactta atttcttttt tcacatcgtc
    13021 tttttcaact acaaaaattg gatatattaa atcttctttt cttacatgat tctctctaac
    13081 catatctctc attgtcgctg atgatctcaa tcttctatgt ctatcaaatt tcatttttag
    13141 cccctacttt ctaaaatctt ttcaattagt gattcgagtg tttgaatttc tgcaattgtt
    13201 actggctgtt gatatgattt aatggtccgt gctgtttgtt ctccaatagc aaaatacgac
    13261 ttgaattttg gtacaaatcc ttcattaaaa taataacgta ctgccgacga acttgaaaat
    13321 gttaatgcat cgatttgttg atgttctatc atttctttaa catcttgtat attttgtttg
    13381 ttaggcactg aagtatataa atctatttta acaacttcat tatcttcaga taacgctgct
    13441 gataacaatg gtctcgccaa ttcactcgaa ggcaaaagta ttttttggtt agtttgatta
    13501 aatgattcta aaaatccttc ttgagaaaag tcgtttggca taaaatcaac tcgaatgcca
    13561 agtgattcac aatattgcgc tgtcttactt cctatcacag caatgttatc aacattaatt
    13621 ccttttagat atttataaaa gaatttcaca gcatttttag atgaaaaaat aagccagtca
    13681 tagcgttgat ttaacaaatg aatatcaaaa tttagtggct ttatatcaat aaaaggtttg
    13741 tgaataattg atactaaatc actttgcatg tcatttgttt gtgtcataac tacaactggc
    13801 ttcatattta aacgtcactc cattatttaa tgttgttcat ttaagcgttt tataatttca
    13861 taagcacctt gctcttttaa tttgttactc actttttttc ctaactcaac cggatctgtt
    13921 ccgttcattg tatattcaaa tcgttcttta ccatctgggg tcataattaa acctgtaaat
    13981 tcgatttctt tttgatctga gattgtagca tatcctgcga ttggcacctg acaactacca
    14041 tccatttctg ctaaaaacgt tcgttcagca gtcacacatt ttgcaacctc atcattatgt
    14101 actttgctta ataatgttaa tagttcttca tcgtcactac gacattctat ccctaaagca
    14161 ccttgtccaa ttgcaggtaa caatgtatct ctatcaagat aagatgttac aatatcatct
    14221 gaccagccca ttcttcttaa accagctgca gctaaaataa tcgcatcata atcttcagtt
    14281 tgtaactttt ctaatcgtgt atctatatta cctctaatcc atttaatctc taaattagga
    14341 tacttagata atatttgtgc accacgacgt aatgaactag taccaataat actaccttct
    14401 ggcaattggg atagtggtgt atgtgtttta gaaatatacg catcaaaagg taattctcta
    14461 tcagggatac aacctaatgt taaaccttcc ggaattacac ttggtacgtc tttcagcgag
    14521 tgtattgcca tatcgatatt tttttcaaaa agttcatgtt gtatttcttt aacaaataag
    14581 cctttgcctc cgactttaga caattgttta tctactatac gatcgccttt cgtgacaatt
    14641 tctttaattt caatttctag atttggctcg acagctttta atttatcaat aaattgctgg
    14701 ctttgtgtta aagctaattt acttcttctg gagccaacga ctaatttacg catgttcaat
    14761 tcctcctagg aacggattgc tctagattat tttcttaatt cacaaaatgt gttgcaaaaa
    14821 ataaattaat catatttaag caaaataaaa taatgttata gtatattaaa taccttgaat
    14881 tcaaccattt gttgattcta agtaaaatat aacttccata taatactgta ataattgaag
    14941 agagtattac cttcgggtca atgaatatac gttcaccaac tgaaattaca ccccattgtg
    15001 tacctaaaat aatactaaat atgagaatta tccacccact taacgttgaa taaaacacaa
    15061 ttgattcaag tgtagcaacg ctacctattc taaagtattt ttgatcaaaa cgcttttcct
    15121 tcaaattacg gtattgcatg atatacagta atgcattgac aaaagctaag gcaaagaaga
    15181 cataacttaa cacagctaga ccaatatgga ctaacagtaa ctcgtctaca acagcaattt
    15241 tctgaacctt attagtataa tgtgtcggtt gaaatgtatt catccctaaa agtgttaacc
    15301 ctattaaatt caagaaaaac acagagaaat tcaaaacttt gataaggtta agaattaaag
    15361 agattgaaat aatcagccaa cttaaagtat agaatacatc tgaaatagac cctaatggaa
    15421 tatgtttagt ttgtataata aaaatagata aagagattgt ttgtaaaacc caaacaatcc
    15481 ccaataaata tatgcctaaa cttctaatct tatgactttt ttggacaaaa tcataaaaat
    15541 agcaaatgat actgattaag tatattaata atataatttc attgaatcga ataaacaggt
    15601 tttcttgcat attatcacca tattaatatg cttattcaaa actaaagata cgtcgcgctg
    15661 aaatttcctt gaccttgctt tctttttgtt gcttcgcttg ctcatgagga catttagctt
    15721 cgatatcaaa tatattttga aatagctcta atttttcatt acttttctta tcactactta
    15781 attctttggc ctgtttaata ggatctttca acatttgatt gatgatactt tttgtatgtt
    15841 tagatataat tttacgttct ctttcgctta accctggcaa tttacgatca atactgtcca
    15901 tcgtttccgc ttgaattgcc atagcttttt cacgtaaagc tctaatcact ggaacaacac
    15961 ccaacatact aacccactca ttgtgtgcat gtatttctgc aggaatttgt tccgaaattg
    16021 ttgcagccgc taattgtcgc tcacgtaagt ttgcatcaac taaacctttt aagtcatcaa
    16081 catcataatt aaagatgttt gtgatggcac taataccagg ttcaatatct cgaggaactg
    16141 caatatcaat caatactagt gaatcttgct ttctattttc tgcaattctt tctatcattt
    16201 catttgtaat gatataagat tgtgcactcg tcgaactaat cacaatatct gcactttcaa
    16261 gtaaatttgg taatgatgat agttcatcat atttcacttg atgctttgct gctaatttca
    16321 tagcattttc aattgttcta tttactactg taatatcagt aattccagaa ccaagaagat
    16381 ttaatagtga taattcactc atttcccctg caccaataat gatagcttgc ttacttttca
    16441 atttgccaaa tacttttttc gccaactcga ccgcagcata agacacactt acagcattat
    16501 cagctatatc tgtttcatta tgtgctcttt ttgcaaaagt aattgcctgt ttaaatagat
    16561 gattaaaaat tgttcctgtc gtacctgtgc tttgcgctaa gaaaaatgca tctcttattt
    16621 gacctaaaat ttgagtttct ccaagtacga ttgaatctaa accagaagtg acacgcaata
    16681 aatgttctac tgcttcgtcc cccactttta cttctgacat tgctttaata tcatctactt
    16741 caaatccaaa tgcacgagct agaaatcgtt gaatatagta acgacctgtg tgaatttgat
    16801 caacaacagc atatacttca gttcgattac atgttgataa tatgacattt tctaaaatag
    16861 atttagtttc atataaatct tcatgggcaa ttcgtaaggc atcatctcta aaagcaactt
    16921 gctctcttag tgcaacatca gctgtgcgat gatttatact aattgcaata aaatgcattt
    16981 gaaacgcccc catatatact aacaatacaa atattataac ataatttaaa atccaattaa
    17041 gtacgatgct taaataaatt ataatctttc taatttactt ttgagttttt gacaaaatga
    17101 tgaacgttga aaaatgttat caattgtgta ctaattaaaa gtttcgtgct tacattattt
    17161 ttaaaatttt gtataagttt tgtcgtacaa aaactatgaa atatacggtt caattaaatt
    17221 ccatatttgt tgttgtttat tattttgaat tgatgaa
//
LOCUS       DATTQF010000046        17173 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.046, whole genome shotgun sequence.
ACCESSION   DATTQF010000046 DATTQF010000000
VERSION     DATTQF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.046"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..585)
                     /locus_tag="VB241_001286"
     CDS             complement(<1..585)
                     /locus_tag="VB241_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244586.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLEQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNMYSYKLISLMIEYLRFA"
     gene            complement(604..1212)
                     /locus_tag="VB241_001287"
     CDS             complement(604..1212)
                     /locus_tag="VB241_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP1244587.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKLEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(1354..1710)
                     /gene="rplT"
                     /locus_tag="VB241_001288"
     CDS             complement(1354..1710)
                     /gene="rplT"
                     /locus_tag="VB241_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP1244588.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(1757..1957)
                     /gene="rpmI"
                     /locus_tag="VB241_001289"
     CDS             complement(1757..1957)
                     /gene="rpmI"
                     /locus_tag="VB241_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP1244589.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(1986..2513)
                     /gene="infC"
                     /locus_tag="VB241_001290"
     CDS             complement(1986..2513)
                     /gene="infC"
                     /locus_tag="VB241_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP1244590.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLISQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(2542..2667)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(2742..4235)
                     /locus_tag="VB241_001291"
     CDS             complement(2742..4235)
                     /locus_tag="VB241_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1244591.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGGNDVATSPFTLVFKNAGFAFAASFINAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFETFGKTNKNGVPYMSLLVTAIIVVIIFVVQSVTEGAYQYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKWFPFGPIFAAILCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(4662..6599)
                     /gene="thrS"
                     /locus_tag="VB241_001292"
     CDS             complement(4662..6599)
                     /gene="thrS"
                     /locus_tag="VB241_001292"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP1244592.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(6824..6919)
                     /locus_tag="VB241_001293"
     CDS             complement(6824..6919)
                     /locus_tag="VB241_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244593.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLK"
     gene            complement(7012..7932)
                     /gene="dnaI"
                     /locus_tag="VB241_001294"
     CDS             complement(7012..7932)
                     /gene="dnaI"
                     /locus_tag="VB241_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP1244594.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMFDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(7932..9332)
                     /locus_tag="VB241_001295"
     CDS             complement(7932..9332)
                     /locus_tag="VB241_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEP1244595.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTAKSTEANPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTLVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEQDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(9333..9803)
                     /gene="nrdR"
                     /locus_tag="VB241_001296"
     CDS             complement(9333..9803)
                     /gene="nrdR"
                     /locus_tag="VB241_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP1244596.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(10013..11038)
                     /gene="gap"
                     /locus_tag="VB241_001297"
     CDS             complement(10013..11038)
                     /gene="gap"
                     /locus_tag="VB241_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP1244597.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(11208..11831)
                     /locus_tag="VB241_001298"
     CDS             complement(11208..11831)
                     /locus_tag="VB241_001298"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP1244598.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEGD"
     gene            complement(11847..12719)
                     /gene="mutM"
                     /locus_tag="VB241_001299"
     CDS             complement(11847..12719)
                     /gene="mutM"
                     /locus_tag="VB241_001299"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEP1244599.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(12735..15365)
                     /gene="polA"
                     /locus_tag="VB241_001300"
     CDS             complement(12735..15365)
                     /gene="polA"
                     /locus_tag="VB241_001300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP1244600.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASGEDAIEKTFEIETSFDNVDFTSLKEATIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDIINYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIYTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGFVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(15658..17145)
                     /locus_tag="VB241_001301"
     CDS             complement(15658..17145)
                     /locus_tag="VB241_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244601.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLSFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQVIILSMIAALIIYLLLR
                     SDRYLHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFANIIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLISICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         5381 a         3268 c         2339 g         6185 t
ORIGIN      
        1 tgcgaacctt aaatactcaa tcattaaact aattagttta taactgtaca tattagattt
       61 caatatatat aataccgttt cttgaaattg aaaatgacca ttgtctatta acttcaaaat
      121 caagtgcgtc tgttccctca ttgataacgt atcaaaatca gttaatgact gtgtcaatcg
      181 tttttcatct tcaattaatt ttgacttagc aaattcttta agaggatgta atgccattct
      241 tgttttgtga tctttgacct catcaataat ttgatgaatt acttctaccg cttcaaaata
      301 ttgttccaaa cctatcaaac ttttcacata ataaatcatc aatgcatcat attgttgtat
      361 gccagttttc aataagacaa ttgtttcttc tcttaattca agaaatgacc ccgtctcata
      421 taacatacgg catttcatca ttgctatttc ttcagtgagt tcaaattgac gttcatattg
      481 tacaatatat tcatacatca ggtcatagtt ttggtcttgt tcagcacgtt ttatatcttt
      541 atataacttt ttcgaagagt taggaaactg aataatatct gacatatatg tcacctcgtt
      601 tgattataaa tcttgaagga ttaatgcgct ccacgtctcg aaatcatcat atgactctat
      661 gttgcctttt tcaacccatt ttattcgtaa agtatctgtt tctttagctt ctacatcatt
      721 cgtacttaca gtgattttaa atacaacacc aatatgtacc ttgcccactt cgttattatc
      781 gtcattaata aaaccgatat attccatatt ttgtgaatct tgctcactta aacctacttc
      841 ttcttctaat tctctctgtg cattaactct caatacttcg ttaatagatt ctgctcctgg
      901 aacatcattc atatgaccgc ctacacctat tgaagattgt ccatgcaatc gagcttctcc
      961 accgccagat aatcgttcat acactaatat ctcgccatgc tcattttcaa gtaaacaata
     1021 agaaatgagt tgtttatatg atggatcctc ttccatatcg ccgcgtcgct taacttcata
     1081 ttgacttagc gcatcaaaaa tattttgacc ttctagttta ttcttattta aaaacccatt
     1141 gaaagtattc ttttcattat taaaaataat ttctctaggt actacaatga tttgttcatc
     1201 aaatttagac atcgcataaa actcctcact ttaatgtttg ttctcttaaa tgcattttag
     1261 caaatatgtc atattttagc gattaaatgt ggcattttgc tatttaaaaa ataaaaaaga
     1321 gatgacaatg ccatctcttt tttataagtg ttattatttt aaagcatctt tagctttagt
     1381 tactaattga gcaaatgctt tttcgtcaga aattgcaatt tctgataaca ttttacggtt
     1441 aatgtcgata ccagcttttt tcaaaccgtt cattaaacgt gagtagctca tttcatgttg
     1501 acgagctgct gcgttgatac gtgtaatcca taatttacgg aagtcacgtt tacgttgacg
     1561 acggtcacgg aaagcgtatt gacctgattt cattacttgt tgcttagcta ctttgtataa
     1621 tgtatgtttt gaaccgaagt aacctttagc taatttaatc gtttttttac gacgcgctct
     1681 tgttactgtt ccacctttaa ctcgtggcat aaaaaattcc tccttaggta attcctaccg
     1741 atttctgtat ttgttcttat tttttgtatg ctaataattg ttttacacgt ttcatgtcgc
     1801 tcttagacac taatctagct ttacgtaatt gacgtttttg tttagtgctc ttgtttgcga
     1861 ataagtgaga tgtgaaagct cttgaacgtt ttaattgacc tgaagcagtt cttttaacac
     1921 gtttagctgc tccgcggtga gttttcattt ttggcataat ataagtcctc ctgtaatagc
     1981 tttaattatt tttcagctgt tggcgctaac ataataaaca tttgacgccc gtccatttta
     2041 ggtttttgtt caactgttgc tatatcttta cattcatctg catatttttc tagcacacgt
     2101 tgaccaattt ccttatgcgt aatggcacgc cctctgaaac gaatagatac tttacattta
     2161 tcgcctttag ttaagaattt acgtccgttt ttcaacttag tttggaaatc atgttcctca
     2221 attgttggac ttaaacgaat ttctttaaca ttgataattt tttgtttctt tttcatttct
     2281 ttttcttttt tctgttgttc gaatttgaat ttaccgtaat ccataattct tgcaactggt
     2341 ggtttcgcat tcggtgcaac gaccactaag tctaaatcta cacgttcagc catttctaaa
     2401 gcttcacgct ttgatttaac accaatttgt tcaccatctt gactgattaa acgtaattct
     2461 tttgcacgaa ttttgtcatt gatttgagtt tgatcttttg ctatggttga cacctccaaa
     2521 atttttacga aatttgcacc aagcaaaaag gaagagcagg tataaaatac ccgctcttcc
     2581 taatacacag ttatgtgtaa tgtgattaac ctgccaactg ctttatgcgt cgctacaggt
     2641 gagaagcggg tgcttctact tggttcgttt cgtattcaac gttgttaatc atatcaacaa
     2701 ttcacattta agtcaacacc ataactgtaa ttatttttat tttaaccttt tatttcatcc
     2761 attgacacgt cttgacgtaa atctacttgt tctaatggaa ttttcttcgt tttatatcga
     2821 agcttatgat aaataaagaa tgctaaaaat actgggattc ccatatacgt aattaagaag
     2881 cgactaaaat taaaatctcc tgttttaata aagtcaacat cttgaccgat gattacaaca
     2941 atacataaaa tagctgcaaa aataggtccg aacggaaacc atttagctgt atatttcaat
     3001 ttagatttat cataattttg tttatcaaat gcccttctaa atcggtaatg actcactgcg
     3061 atacctaccc aggcaataaa gccagttaac ccacttgcag caacgatgta ttgataagca
     3121 ccttctgtaa cactttgtac cacaaatatg atgactacaa taattgcagt tactaataaa
     3181 gacatatatg gcacaccatt tttatttgtt ttcccaaatg tttcaaacgc caatttatct
     3241 ttactcattg aataaagcat acgcgttgag gcatacattc cagagttacc tgctgataaa
     3301 actgatgtta agataacagc attaataaat gaggctgcaa aagcaaatcc tgcattttta
     3361 aaaactagcg tgaacggaga ggttgcaacg tcattaccgc ctcccattaa tgcactacta
     3421 tcataaggaa ttaacatacc gataacaaaa atggctaaaa tgtaaaataa taaaattctc
     3481 cagaatactt gtttaatcgc tttcggcaca gcacgttcag gattttctga ttccccagct
     3541 gtaataccta ttaactctgt accttggaat gaaaaaccag caattaagaa tacacctaaa
     3601 atagataata aacttccgcc tagatttcca ccaagaatag gaccttcacc tttattaaat
     3661 atttcgaatc ctacaacatg accacccatg attccgacaa tcgttaataa accaattgca
     3721 atgaaaacaa taactgtaac cacttttatc aatgccaacc agtattcact ttcaccatag
     3781 acgcgaaccg ataacgaatt cagactaaaa attataacta agaacaacgc actccatgcc
     3841 caagctggta tgccttgtaa tggtgtccaa tattgaatga cttgtgctgc aatcgtaata
     3901 tctgctgcta cagttactac ccagttaaac caatagttcc aaccaagcgc aaaccctaaa
     3961 gatggatcaa caaatcttgt agcatatgta ctaaatgagc ctgatactgg caaataagta
     4021 gccatttcgc caagtgacgt cattaagaaa aataccatta ttccgataat tgcgtatcct
     4081 attaatgcac ccaaagcacc tgcatcatga attgctccac cagaagttac aaataaacct
     4141 gtaccaatac aacccccaat cgcaatcata gaaatatgac gatctttaag tccccttttg
     4201 acaacattgt tactttcatt ttgaactttt gacatataag ctccctttcc atgttttaga
     4261 ggccaagtaa taaataacct ttatttactt tcataccctt tttttacaga atatagtgat
     4321 attaaaagta aaaaagtggc tacatacctt taagatatgc aaccactttg gcaattatct
     4381 atataaggta gcacatcaca ttctgtgaca gtacagttcc tattcgaaaa ctgtcccaat
     4441 agatataatt tatggtttat atctatttcg gcatctttac ctttcacttg ttcaacttat
     4501 gtaccataaa tacttctgac aagttactaa ttaaacatgc aacctctaac tcaatttaat
     4561 attttaacta acttgtaata tacaggattc atcacgcata atcaaccctg taaaacttga
     4621 tacgcaataa aagtttcaaa gcattttatt gcgacaactg tctatctatg ttttttcaaa
     4681 cgaatttcat caactagatt ccagataaat tcatcttttt caactgtttc ttggtcttgt
     4741 gatccatatt gacgcacatt cacttgatta ttttcaactt ccttatcccc aactacgatt
     4801 tgataaggta ttttttgcat ttgagcttct ctaattttat aacccatttt ttcattacgg
     4861 tcatcaatac ttacacgaac gccttgagat ttcaattcat cttgtaattg gcgcgcataa
     4921 tcataatgta aatcaacgtt aactggaatg atttgaactt gttttggcgc taaccaagtt
     4981 gggaatgcac ctttagtttc ttcagttaaa aatgctacaa atcgttccat tgttgataca
     5041 acaccacgat gaataacaac tggacgatga tgttcaccat cttgaccaat ataagttaaa
     5101 tcaaaacgtt ctggtaataa gaaatcaagt tgtgctgttg ataatgtctc ttctttaccc
     5161 atcgctgttt taacttgaac atctagtttc ggaccataga atgccgcttc accaatcgct
     5221 tcttcgtacg ataagccaag ctcatccgct gcctctttaa gcatattttc agctttattc
     5281 cacatgtcat catcatcaaa gtacttttct ttatcttcag ggtctctata acttaatcta
     5341 aagctataat cctcgaaacc aaagtcttta tatacatcaa taatcatgtt tacaacgcgt
     5401 ttgaattctt ctttaatttg atcaggtcga acaaagatat gtgaatcatt taaagtcata
     5461 cctcgaacac gttgtaatcc tgatacagca ccacttgctt catatctatg catagttcct
     5521 agctcagcga tacgtatagg taattcacga tatgagtgtg gtttattcgc ataaatcatc
     5581 atatgatgtg gacagttcat tggacgtaat accatagatt cagtttcatc taactgcatt
     5641 ggtgggaaca tatcttcttg atagtgatcc cagtgaccag atgttttgta taaatcaaca
     5701 ttagcaagta cgggtgtata aacgtggtca taccccatgc taacttcttt atcaacaatg
     5761 taacgttcaa tttcacgtct aattgttgca ccgttaggta accataatgg caaaccagca
     5821 ccaactaatt ggctatttgt gaataattct aactctttac caattttacg atgatcacgt
     5881 tctttacgct cttctaacat ttgtaaatgt gcttttaatt cttttttatc aaagaaagca
     5941 gtaccataaa tacgttgtaa cattttgttg ttactatctc cacgccagta tgcacctgct
     6001 gtagataata gtttaaactc tttaatttta gctgttgatg gaacgtgaac tccacgacat
     6061 aaatcagtaa aatcaccttg actatataat gttacatttt catcttcagg aatcgcgtcg
     6121 attaattcta atttgtattc atcattgctg aataactctt tcgcttcatc tcgtgaaacc
     6181 acttttcgtt cgattttcat attttcgtta acgatttgtt tcattgtttt ttcaatttgt
     6241 tcaaagtcat cagatgagat gttttggtca atgtcgaagt catagtagaa gccaccttct
     6301 attacaggac ctacaccaaa tttaacatta ccatataacc ttttaatcgc gtgtgccatt
     6361 aaatgtgcag tagaatgacg taatacctct aacgcttctt cactacctgg tgtcacaatt
     6421 tcaattgatc catcagtttc aagcggttta gttaaatcta caagttgccc gttaaatttg
     6481 ccggcaacag cttttttacg taatccagga ctaattgatt gtgctatatc ttcagtagta
     6541 gtacctttat caaacgcctt tttattacca tctggaaatt gaatattaat ttgttccatg
     6601 ttaaaccctc ctatttgctt tataaatact tttatctgac ctcgttaaaa aattgaacaa
     6661 caatcatcgt tttaacatta aacctattaa caataaacag agaatttatg acaccaacaa
     6721 acatttatgt ctgaaaaaaa caaaaaatcc cgcccctata caagggacga gatcgtcgtg
     6781 gtaccaccct agttatttaa tacaagttat gtattaaata tctctactta agataacggt
     6841 cttgatccgg gtattcatta caaataccat aaatgaagta gtaatcatct aatttattaa
     6901 ccatattctc atcaaacaat ggctttctgt gtaatattga ttagatcatc ttgtcttcaa
     6961 tcatttaaac gatttagatt tgtattcatt atacaccaat cattttaaaa ttcaattgtt
     7021 tctgaaattt tctcctgata aaaagtatgg tgttgacaaa gatttgacac gttcaataat
     7081 acgtgctgct ttagtcttct cttcaccatc acgagtcatc gctaaatgat gttccaattc
     7141 actatagtca aaattagaac taaagaatgt tggtaattca tgaaccattc gataatgtag
     7201 caaaggtcca attacctcat ctctcaccca tggagtcact tcttcagccc caatatcatc
     7261 aaacattaaa atgtttgctt ctcttacgcg atgtaatttc ttttcaaaag aaccatcttt
     7321 aaagccacct tttaatgttc taataaattc cggtaaataa ataattgtcg aacgtacctt
     7381 cttagatttg agctgattcg caattgcacc tagaataaaa gatttacctg tcccaaatgg
     7441 accataaagg taaaggcctt tcacttgttc cccattagtt attgctgtac aaatatcatc
     7501 tgctgccata gctacatcaa gacggtctcg atgattcata taaatatctt tcaatttggc
     7561 gtttaaagta tctcgttgca tatgatgaga tgtaattagc tcagcttcaa agcgttcttc
     7621 gtcgtacttg attttacatg gacattgtaa atagcgtatt ttaattcggt tattatcaac
     7681 atataactca ggcacatgcc cctttacgaa atttggacaa tcagcaaatt tatgaccgtc
     7741 ataatgtttt tgttgatctt tatactcttg taacacattt aagtcttcat caatcatagc
     7801 attcgttaat tcagctcgat gcgcttccaa aaattgctta acatctgggt cattgattac
     7861 ttcttttttt atcttttcta ttcttttttc aaagtcctgc gacgtgttaa ttatactttt
     7921 aaattgcttc attattgact gtcctcctcc cattttttag ataatttatc tagaaatgct
     7981 tgtcgatctt gctctaattg ttgatcatct acgctattat ctttagccga atcttgttca
     8041 ctaggtttat ctctattttc taaccattta ggtgtttttt cttttgaaat gcgattacgc
     8101 tgcccatagt atgaaccacg cttttggtaa tttccgctag aaccctcatt tttaggttga
     8161 ttaacttttt tagcgtaatt atatgcttct ttagctgtct taataccttt tttcttccaa
     8221 tttgatgcta tttccaaaat atacgcttta ggaagtttca tatcttcttt taacatgaca
     8281 aattgcaaca aaatattaat gacaccaaaa gacatttttt cacgttcaat taattcttca
     8341 accaatgtct tttgcgatat agttggttct gattcagacc aagaagctaa catatcaatt
     8401 ggactcgttt gttcaagtaa ttcaaaccat tcatcacttt gtggctttgg attcgcttct
     8461 gtagatttgg ccgtcgaaga tgatgtagca ggagatttca cctgtaattt aggcatttga
     8521 ttttcgtgtt ccattaagta atacgagcgt gcttgtttac gcatttcttc aaaggataac
     8581 tgttgtccac ttgtaattga atttaaaata acatgcttca tgccatctgc tgttaaacca
     8641 tataaagtcg cgagttgtgt aattaaacgc tttgcatctt tggtaacaat gtcttgacta
     8701 ataaaatgtt tacctaacat ttgtctcaac atttcaaaat caaaagattc atttgataaa
     8761 tcgattcctt ggtacggttc attaatcgga atatcacttg tatcgatatc tatttttgta
     8821 gacggcactt taaaaacatc agtaaattgt ctcgttacct gtttaaattc actcaaatca
     8881 atttgttggt actcaaagta tttcttcaac tcatgaaatc gacgatgctc gacttcacta
     8941 tataaaaaga ttgacaacat tggatcatta aaaaataaat gtgctgaagg cggttgaatt
     9001 aattggtaaa caaattgtgt ttcttgttca tcatgtttga caaacgcctt taacaatcca
     9061 atcgcttcaa gtaagtccat ttgttgtcta aactctagta aattaatttt aagttcattc
     9121 ataaaaatat aatgagaaag aatcaatgtt tcattatgac tttctttaac gaattgagtc
     9181 ataaaatggt ataaacccac tgcttgtgtt ccaattagcg gtgtatacag tcgattcaat
     9241 acctctaaat gattcgtatt taaatcaaag tgttgcataa ctttgaattg atcctttggt
     9301 cttaagccga attcaaaggc ttgtcgtccc atttaagcat cactccgttt gttttcgctt
     9361 aaaatccctt gcatcgatgc taacaattga tcaacatctt taaattcttt atagactgat
     9421 gcaaatctaa catatgaaac ttgatcaaca tgcattaaca agttcataac gtgttcacct
     9481 atatctcgtg aagacacttc cgtatgacct tcatctcgta attgccattc aaccttgttt
     9541 gttatgtctt caagttgttg atatctaact ggtcgtttct cacaagaacg cacaagtcca
     9601 ttaagtattt tttctcttga aaactgctct cttgtgccat cttttttcac aactataagc
     9661 tgactaactt cgatatgttc aaatgtagtg aaacgtgttc cacaattttc acattctctt
     9721 cgtcttcgaa tggcatttaa ttcatcggca tgccttgaat ctacaacttt agattgtgta
     9781 gaattacatt tcgggcattt cattacatca ccctctttat tttgattatg cctaattata
     9841 ctataaatct agagatgaaa aaagaatccc tcaatttaat tcatttaacc aaataatgaa
     9901 acaataaaaa acattatatc gttacttatt aagtaataag catgacaata ttattatatt
     9961 aaaaataaaa aacctaactc cgaggtcaga gttaggctat aaattaattg tattaacttg
    10021 cacttacagt ttcttttgat gtcaaaagtg ctccaatttg ctcagcgaca tctacaactc
    10081 tatttgaata accccattca ttatcatacc aagcaataac ttttacttta ttccccgaca
    10141 taaccattgt tgattttgca tcaataatag ctgaatgtgg attagtatta aaatcaacag
    10201 acactagtgg ttgatgttcg acttcaatga taccttctaa acctgcattt tcaaaagctt
    10261 ggtttacttc ttctgcagtt acttcttttt ctaaatcaac aactaaatca acgagcgata
    10321 cattctttgt tggtacacgt aatgccatgc cgtgtaattt accttctaat tctggtaata
    10381 cttcttttaa agctttcgcc gcaccagtag aagtaggaat aatgctttca ttacatgagc
    10441 gtgcacgtct taagtcttta tgtggattat caatattttt ttggtcattt gtaatagcgt
    10501 gaacagtagt cattaaacca ttaactattc caaactgatt atttaaaact tttgcaactg
    10561 gaccaatgca attagtagta catgaagcat tactaaaaat gtcaaatgct tctatatcta
    10621 attggtcatc atttacgcct ttaactacca tttgaacatg tccacctttt gaaggaccag
    10681 ttaacaatac ttttttggca cctgctttaa tatgtgcgat ggctttatca ccatgattaa
    10741 atttaccagt tgcatctata gcaatatcga tatctaattc tttccatggc aagttttcag
    10801 gattgcgatc agcaaccaat ttaattttgt gatctccaac ttgcaatcca ttttcaatcg
    10861 gttctacttt tagattatat tttccatgtg ttgtatcgta attgattaaa tgtgcaattg
    10921 tttcgggtgg ataactagca tttatcgcta ctacatttaa atttttattt tgtaatgcaa
    10981 tacgtaatac cattcttcca attctaccca taccattaat tgcaatattc gttgacatta
    11041 aaagcacccc ttgattttta tgtgttatac tttatggaat tagtatagca tattatatga
    11101 aatttgaaag cgttttccgt tgatttattt atctttttta taaaaaatag tcattactta
    11161 catacatcca cgcatacgta aagtaatgac ttatttatag agtaatatta atctccttct
    11221 ccgtaattcg gcttttcaat ataaccttct tcttctaaca atctctcaag gttttgtttt
    11281 aattcaagtt tatcccctaa attatcgata acatgatcgg ccattcggct ttttttatca
    11341 atagaaattt ggctatagac acgagctttt gcatcttcta atgacaaatt attacgttgc
    11401 attaaacgat ccatttgtat actttcagaa gtgtatacaa cccacacttc gtctactgta
    11461 ttttccaatt cattttcaaa taataatgga atatccatga ttacattata tccttgtttt
    11521 aaatattctt gcttttcttc ttccataata tctcgcacga taggatgtat gatagcattt
    11581 aattctaagc gtttttctgg atggttaaac actaaatcac ccatataacg acgattcatc
    11641 tcaccatttt catcaattgc ttcatcacca aagacttctc gtacttgagc taaaccttta
    11701 ctcccctttt taacagcttc cctggctgct ttatcagcat ctactacttt aaaaccgaat
    11761 acggataaga gttctgatac tgttgatttt cctgaggcga ttccacctgt tagaccaata
    11821 actttcggca taatttcact ctttctttat ttttgacata ctggacaata atgactattt
    11881 cttgtcgcga tgatttttgt ttcaatttga cttccacaca ctttgcatac cggctgctta
    11941 tatacattga ggtgcaattg catctcacca gtttttccat cagcatgacg ataatctgaa
    12001 atacttgtac cgccatgctt aataccttct tctagtactt ctctaacata ataaaaaacc
    12061 atttcttgtt gttggtgtgt taagtctttt acttttttat ctggtaaaac acctgcacga
    12121 aacaacgctt cacatgcgta aatatttccg caacctgcga ttactttatg atccaaaatc
    12181 acttgtttga ttggtttatt cttattagac tgttgatgaa ttcgatttaa ataatacgtc
    12241 aatgcttcat ttgtaaaagg ttcaggcgct atttccagaa atgaaggata agatgctaca
    12301 gacgcaacat ttctaatttc tccaaaacga cgtatatctg aataaattaa ctttttgtca
    12361 tttgacagct caaaaataac atgccaatgc ttacgataat taggtatcat aatatcttca
    12421 agttcatcta caatgaaaaa accacccgcc atacctaaat ggctaattaa tgtacgttgt
    12481 tctcgtttat tatctagttg aaaaacgata tatttacttc ttcgttctac attagtaatg
    12541 gtatagcctt ccgataaagt tttaaaagta tctaattcaa ttccttttat aattgtttcc
    12601 ttgccttgag ctttaccttc gattacttta tccgaaaata taacgtgttc aattttctga
    12661 tttataacgt agggttcaat tcctcttttt acatgttcta cttctggtaa ttcgggcaca
    12721 ccattaacct cactttattt tgcatcatac caagttgcac cataacttga gtctactttt
    12781 aatggaacat ctaattgcaa tgcattttcc attatctctt ctacaaattc actaaatgaa
    12841 tctacttctg acttaggtac ttcaaaaatt aattcatcgt gtacttgtaa taatagttta
    12901 gcttgatatg ttgtctcttt cattttttgc gcaaatttaa ccattgccag tttaatgata
    12961 tctgcagcac tgccttgtat tggcgtattc atagcagtac gttcagcaaa gccacgtaaa
    13021 ttaaagttac gactcgtaat atcaggaata tagcgtcgac gatgtagcaa tgtttccacg
    13081 aaacctaaag ctttggcatc ttttacaata tcagacatat attgttttac acctgggaaa
    13141 ctagctaaat aatcatcaat gaatgctttt gcttttttac gagtaatacc taaactttga
    13201 cttaaaccat aatcacttat cccataaaca attccaaagt taaccgcttt tgcttgacga
    13261 cgcattaaac tatcgacttg atcagcttct acaccaaata ctttcatagc agttgctgta
    13321 tgaatatcat cgccgttgat aaatgcttct ttcatactct catcttgtgt aatgtgtgct
    13381 aatacacgca attcaatttg agaataatct gctgataata taacgctatc ttttgaagtt
    13441 ggtttaaagg cttttcttat tttacgccct tcttcaagtc taaccggaat attttgtaaa
    13501 ttaggatcta cacttgataa acgtccagtt tgcgctaaag tttggttaaa acgtgtatgg
    13561 atacgttgat catcactaat taccttttgc aatccttcaa cataagtaga ctgcaactta
    13621 gataattgac gatattctaa aatataatca atgataggat gttcaccttg caattgctct
    13681 aagacatcta cagcagtaga atatcctgtt ttcgtctttt taataacagg taattgtaat
    13741 gtctcaaaca atacaacacc taattgctta ggagaattta tattaaaatc ttcaccagct
    13801 gcatcatgga tatttcgaat caagacgtct aatttttctt gaatttcttt ttccatttct
    13861 tctaaatcat gaacatctgt atatatacca atttcttcca tttcacttaa aatcttagct
    13921 agcggtagct ctaaatcagc taagagttct acctgattgt attcttctaa ttgtttatcc
    13981 atatttggtt tcgcaaagta aattgcatca gtaatagaag caacatatgg atttaaaaca
    14041 tcatcttcag gtaccttaaa tttcttacct tttccatata tactcacatc gtctttcaca
    14101 aaactttgac cgtacaatga aacaactgat tgaacatcac taatcgtacg agatggatca
    14161 attatataac tggccaacat aatatcgaat gaaatatttt gaatatcaat tcccaatcta
    14221 tgtgatgcta catatgtttt tttagcatca tatacgactt ttttcgtatt cggattttct
    14281 aaccatgaaa ctagttcagc ataattaatt atgtcatccg cattaattac aatatgtttc
    14341 tcacctgtaa ataaagagaa ttttaaaata ttatttcgca aatagttgcc accgtctaat
    14401 tcgaaatgga tggtcgcttc tttcaatgaa gtaaaatcaa cattatcaaa agacgtttca
    14461 atttcaaatg tcttttctat tgcatcttca ccacttgctg attgatcaat gtcagccaac
    14521 aattgtttga attctaactt cttaaacaat tcgatttttt cttgttgttc atcttgatga
    14581 gtcattaacg tatcttcaag ttttacttca atcggactat ctacattaat cgttgctaat
    14641 tctttactca ttaatgcatc ttctttgctg ttttgaagtt tttcttttaa ctttttacct
    14701 gaaatttcat ctaaatgttc ataaacacct tctactgtgt caaattggtt cagcaatttt
    14761 attgctgttt tctctccaac acctgcaaca cctggtatat tatcagaagt atctcccatt
    14821 aatcccttca tatcaataat ttgattaggt gttaacccgt tgtatttttc agcaataaag
    14881 tcaggtgtat aatgatcaac atcagtaaca ccttttttag tgtaataaat ggttacatta
    14941 tccgttgcaa gttgtgttaa atctcggtct cccgtaataa taattgtctg aaagcccgct
    15001 ttatctgctt ctttacttaa agttccgata atatcatctg cctcatagtt atctaattca
    15061 taacgtttaa tatgataagc atctaataat tggcgtatat aaggaaattg ctcacttagt
    15121 tcaggcggcg ttttctggcg tccaccttta tattcactat atttttcatg tctgaaagtc
    15181 gttttacctg catcaaacgc tactaaaaaa tgatttggct tttcttcttt taaaatcttc
    15241 tctagtaaca ttgcaaaacc atatactgca ttggtatgaa tgcctgcttt gtttgataac
    15301 aaaggtaatg cataaaaagc tctaaaactt aagctattac catcgattaa tactaattta
    15361 ttcacaattt taacctccag aactaattta tatatatttt agcatattcg actgtgatgt
    15421 cgtaattaaa gaaactatct catagagtat ttatacacga cctaatttca aataaaacaa
    15481 gtgactatat agactaagct aaaaaataaa aagacataat catgattgtt cttgatgagc
    15541 actcaataaa gtcaggttaa aagtccaact tttgagatgc acattatcat ttcatgatta
    15601 tgtctacttt ttaatatttt tatgctgcta ttataaattt ttataggtaa gaataagtta
    15661 tgaatcgtca gaaccaaatg gaatatattt aactttggtc attttcggta attcttcata
    15721 attattaaac cactcttggt aatttttatt gattggtgac ggatgatgca tataatgttc
    15781 aaacggcaat gattccactg tataaacctc ttgtcgtgga tgatctttaa tttgttgttt
    15841 taataactga actctttttt catgttcata atgaacataa ataaatgcac taagatatat
    15901 cacagctaaa actagtgatg cacctttaat aaaattaaca ttgattgact tatatttccg
    15961 aaattctttt aataaaataa ttaaaatgat tacatgtatc gtataaacaa tcaaaaaatt
    16021 acctggttca attggagtaa caatgactag tgtcgacgcc gaaacacata ttgaaataag
    16081 taaggaatat aaagtaattt gtgttttacg atcattaata gacaaataaa tacctacaaa
    16141 tatcgaaaac gcaaagtaac cacatacaat tatgttcgca aaaccaacca atccaatatc
    16201 tgtattttta tttaataaaa actgattgta aaatagtaaa taataaagcg gtaaagtgat
    16261 aaatcctatc ataatgatac gacgctttaa tattgttaaa tgtaaatacc gatcacttct
    16321 aagcagtaga taaataataa gcgctgcaat catacttaaa attataacct gactgaaaat
    16381 gacaccatat ggaagtgatg tcgatatcat ttctgcaaat tttgaaaaga taccttgatt
    16441 attagaaact tgttgatatt ctgatccttc aaataaaatt ttgcgataat ttggattgga
    16501 aaacataatg attgtaccta tagtagcaat cataaatgaa aacaataatt tataattaag
    16561 tgttcgattt gcagtaaatt catacagaaa agctaaaatt aaaatgatac agtggaataa
    16621 tgtcacattc tccataaata attgaccgaa gaagcttaaa acatagaata gtactgtaac
    16681 acttaccggt tgcttttctt tatatataat tgcattaata caataataaa tgataaagag
    16741 tgaaattagt gttgatgtcg cataattata aaatcctgca aaccagccat atgtatctgc
    16801 ataaatagca cttggtaaaa ttaacatcaa agaaaatgct aacaaataat agcttgtcca
    16861 cgctttacaa cgtgtaatgt gcataatcat ccaaatgata cccatactaa tgagaccata
    16921 tgaaagccaa cgtaaccagc ttacatgtac agctataatt tcaaagatat ttccgatata
    16981 gcgaccatta agtgatgcaa atcctacttt taaaatatca gtattataat tgctaaacca
    17041 ttgtaaatca tcgtgcatga gtggtagtaa gatacccata aaagtataaa acaataatat
    17101 cgcaattaat atcaaagttg tcttgtgtaa ttgaattgtt ttcactttgc taatcctcaa
    17161 atctagttaa att
//
LOCUS       DATTQF010000047        16623 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.047, whole genome shotgun sequence.
ACCESSION   DATTQF010000047 DATTQF010000000
VERSION     DATTQF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.047"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(181..1179)
                     /gene="sirC"
                     /locus_tag="VB241_001302"
     CDS             complement(181..1179)
                     /gene="sirC"
                     /locus_tag="VB241_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HEP1244602.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMVGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMVILLVFQTKGHIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTFILGMVIVAVIPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLISSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1176..2171)
                     /gene="sirB"
                     /locus_tag="VB241_001303"
     CDS             complement(1176..2171)
                     /gene="sirB"
                     /locus_tag="VB241_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HEP1244603.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALTGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYLMKKGRQRL"
     gene            complement(2187..3179)
                     /gene="sirA"
                     /locus_tag="VB241_001304"
     CDS             complement(2187..3179)
                     /gene="sirA"
                     /locus_tag="VB241_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HEP1244604.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDSKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3410..4390
                     /gene="sbnA"
                     /locus_tag="VB241_001305"
     CDS             3410..4390
                     /gene="sbnA"
                     /locus_tag="VB241_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HEP1244605.1"
                     /translation="MIEKSQACHDSLLDSVGQIPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4387..5397
                     /gene="sbnB"
                     /locus_tag="VB241_001306"
     CDS             4387..5397
                     /gene="sbnB"
                     /locus_tag="VB241_001306"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HEP1244606.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDTPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5418..7172
                     /gene="sbnC"
                     /locus_tag="VB241_001307"
     CDS             5418..7172
                     /gene="sbnC"
                     /locus_tag="VB241_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HEP1244607.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSEITKENGQTI
                     LIYKRETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTQMIHGDTANIDELESLTAPIKEQATDMLHDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEIREKLVVPLPLKFGDYLSASSMRSLINIAAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAERLLRQLIEKDAMLAKYVTVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KKDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7165..8421
                     /gene="sbnD"
                     /locus_tag="VB241_001308"
     CDS             7165..8421
                     /gene="sbnD"
                     /locus_tag="VB241_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HEP1244608.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNVEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGIVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8411..10147
                     /gene="sbnE"
                     /locus_tag="VB241_001309"
     CDS             8411..10147
                     /gene="sbnE"
                     /locus_tag="VB241_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HEP1244609.1"
                     /translation="MQNKELIQHAAHAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGQFAYWSAMGHHMYHPEVWLIDGKSKKLTTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYAEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPIHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPTSKLSQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPEVCYVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWKLV
                     AHRLMTWKKEYANNAVFIDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10056..11906
                     /gene="sbnF"
                     /locus_tag="VB241_001310"
     CDS             10056..11906
                     /gene="sbnF"
                     /locus_tag="VB241_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HEP1244610.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMYQLVSS
                     LIYENIVVYKASYQDGVGYFTIEGNDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEKLQWKWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGITDATTISET"
     gene            11881..12657
                     /gene="sbnG"
                     /locus_tag="VB241_001311"
     CDS             11881..12657
                     /gene="sbnG"
                     /locus_tag="VB241_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HEP1244611.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDED"
     gene            12657..13859
                     /gene="sbnH"
                     /locus_tag="VB241_001312"
     CDS             12657..13859
                     /gene="sbnH"
                     /locus_tag="VB241_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HEP1244612.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLVFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHQF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREIQIEAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13863..14627
                     /gene="sbnI"
                     /locus_tag="VB241_001313"
     CDS             13863..14627
                     /gene="sbnI"
                     /locus_tag="VB241_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HEP1244613.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14823..15449
                     /locus_tag="VB241_001314"
     CDS             14823..15449
                     /locus_tag="VB241_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1244614.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISNGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVSTSDTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEVHKDEATTKVDSDNTEPPIESKDHVSKKD"
     gene            15660..16436
                     /locus_tag="VB241_001315"
     CDS             15660..16436
                     /locus_tag="VB241_001315"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEP1244615.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
BASE COUNT         5555 a         2689 c         3314 g         5065 t
ORIGIN      
        1 gattgatatt atagaatatg agtcggtgtt atttgtatat aaagtattaa tatgtaatta
       61 agaaagtcga cgcttgttaa cattaacaac aactatatgt ataaaaaaac ctcgcaactg
      121 ttagttagct gaatcatttt gctaacgttg ttgtgaggtc attttgtttt aataatatct
      181 ttataacttt ttcacggtta ataataagta tatgaagaat ggtgcaccaa acgcagcaat
      241 gaatacacct gccggcactt ctttaggcaa gaatagggtg cgtccaatta agtctgcgat
      301 aacaattgat atggcaccaa tcattgctga cattagtaac tttttagcat aacttccgcg
      361 aacgattgtt ttcgcgatgt gtggtgcaat taaaccgaca aacccaatat ttcctactaa
      421 actgattgcc atagatacga gtattgtaga agagattaat tgaatcagct tcatacgttg
      481 tacatgtaaa cctaagccaa tcgctacagg gtcatcaagt atagatattt tcattttagg
      541 tataacaaga aataacaacg gaataacagc tacgattacc atacccaaaa tgaatgtatc
      601 tttaaacgtt gcaccgtaaa gacttccgac taaccaagta taagctttgg ctgcagatag
      661 ttgctttgtt gtaataagta gcccttgtac aagtgcaata aataatgttt gcattgaaat
      721 accgatgatt atgagtgttg tcggtcgaat gtgacctttc gtttgaaaca ctaatagaat
      781 caccattgca attgcgccac ctaatacagc aaatagagga agtaaatgta tcgttaaatg
      841 gctgaaaaat gcaataaaga caacagcact taagctagca ccacctgtga ttccgataat
      901 atctggtgag gcaattggat ttttcaagac attttgtaac attaaaccgc tcatccctag
      961 tgctgcacca gctaatatcg caagtgtaat gcgaggtaag cgtaatactt ctaaagtgaa
     1021 ttgatctata ctgtcatttg gatttataaa gtacatcagt acgcgttgta atggtataaa
     1081 gcttgaacca accatcatac ttaccactga aacgatggct aaaaagatta acgcgaagat
     1141 gagatggtaa ttgtcctttt tattaatctt ttcggtcata agcgttgacg tcctttcttc
     1201 ataagataga ttaagacaat agcgccaatg acagcggtaa cgacaccgat aggcaactct
     1261 agtggcttaa taataatacg agcaacaatg tctgagataa tcattaggat tgcgccggtt
     1321 aatgcagtaa aaggaattaa atacttatag tttggtggta ataatcgttt actaatattc
     1381 ggtacaataa gtcctacaaa gacgattgat ccagctacag caaccgagat gcctgctagc
     1441 atactaatga gcattataat catccatttg attaatttta tgttttgtcc gagtccagtg
     1501 gcaatgtcgt cacttgtcat caagatgttg atgtgtgcag ccatgctaaa tgcaattaaa
     1561 ataagtatca atacaagcgg aataatccat gggatatccc aaatgttacg taatgaaacg
     1621 gagccactca accagaataa taggccttgt aagtctgttt cattcataat gagaatgcct
     1681 tgagtaaaag ctgtaaataa catcgcaatc gcggcacccg ctaaaatgac acggtgaggt
     1741 gaaaataggg tttgtctaaa catccctagt gcaacgacta agactgtaac aacaatagca
     1801 cccaaaaacg cgatgacaac aatcatttta aaagattgaa tttggataaa tgtaatacta
     1861 aagatgacaa aaaatactgc gcctgcattg acaccgaaaa gccccggcga tgctattggg
     1921 tttcgtgtaa gtgcttgcat caacaaacct gagactgcta gtgcggcgcc tgtcaatagc
     1981 gcaatgattg ttctcgacgc ccgtgcacca gtgacaacat catgtaaatc gttttcacta
     2041 tcaaagttga ataacgcctg tatcaccgta cctggtgaca caagcgtatt tccaatcatt
     2101 aaacttaaga tagctactat tgcaagacat aaaccagcaa taacgatttg gtattttggt
     2161 ttaagtagca tcgtaaaact ccttaattat tttgattgtt tttcaatatt taacttttca
     2221 tataaatcgt caataagttt taatgatgat ttatatccgc cagctaagtt ccaagtgatt
     2281 tcatctaaat catcagatac ttggttgttt ttaactgcgt ctaaattttt ccactcttta
     2341 cttgaagtcc attcgctttc agtcttttta actaatgcag catctttcgc atttggatct
     2401 gattttacta caaaaatatg atcagcgttc attaatggaa tgctttcttt agatgtaagt
     2461 tggataatat ctttaccatt atcaacttgt ttttgtaagt ctttattacg tttgaatcct
     2521 aaatcattta agatttcacc agcatatcca ccagcataaa ttcttgtatg atcagcacgg
     2581 aagttaacaa ctgaagcttt caatggccat gcatctttat actttgcttt tgcatctttt
     2641 tggaatgcag ctactttatc atcgtacttt ttaagtaaat cttcagcttc tttttctttc
     2701 cctaaagctt tccccattaa cttagttgta tctttgaatt tgaaaactgt atcagtagaa
     2761 actgttggtg cgattttaga taattgatcg taaacttttt catttctaac ttttgacgcg
     2821 acaattaagt ccggttttaa tttagagatt tcttctaagt taggtgcagg ttcttgacct
     2881 acaatcttag tatcttttaa atcatttttt atgtattcga atttcggttt ttgtgtccat
     2941 gattctacag cacctacagg tttaacacct aaagatacag cgacgtcagt ggcaccttga
     3001 tatagtgtaa caacgcgctt tggtttccct ttaatttcag ttgtacccat tgcatgttta
     3061 attgaagttg tttccttatc tttgctatca gatgattgct tatttgaatt cccactacat
     3121 cctgctaaaa caagtaggaa agcaagcgta acaacaagca ttttaattac tttattcatt
     3181 gactaattag cctccttcgt gatgtatgac aatgagaatc attatcacgg tttagtatga
     3241 attaaatttt ttcctaagtc aataaaatat ttatgattta catgcaactt ataattattt
     3301 gacatataaa tgcataaaaa atataatcct aattacttga tagtgagaat cattatcaat
     3361 taggtaacac acaatattat agaattttaa atttgaggag gaagcgcttt tgattgaaaa
     3421 aagtcaagca tgtcacgatt cattgttaga ttctgtaggg caaataccta tggttcaact
     3481 tcatcaacta tttccgaaac atgaagtgtt tgcaaagtta gagtatatga atcctggagg
     3541 cagcatgaaa gatcgacctg ccaagtacat cattgaacat ggtattaaac atggtttaat
     3601 cactgagaat acacatttaa ttgaaagtac ttctggtaat ttaggcattg cgttggcaat
     3661 gatagctaaa atcaagggat taaaactcac gtgtgttgtt gatcctaaaa tatcaccaac
     3721 aaatttgaaa attattaaaa gttatggtgc caatgtagaa atggttgaag aacctgatgc
     3781 acatgggggt tatttaatga ctcgtattgc aaaggtgcaa gaactgttag ccactattga
     3841 cgatgcatat tggattaatc aatatgcgaa tgagttaaat tggcaatccc attatcatgg
     3901 tgcaggcaca gagattgttg aaacaattaa gcaacctata gattattttg tcgcgccagt
     3961 cagcacgaca ggtagcatta tgggtatgag tagaaaaatt aaagaagtgc atccaaacgc
     4021 acaaattgtt gctgttgatg cgaaagggtc agtcattttt ggtgacaaac ctattaatag
     4081 agaattacct ggtatcggtg ctagtcgtgt acccgaaata ttgaatagat cagaaattaa
     4141 tcaagtgatc catgtagatg attatcaatc tgctttgggc tgtcgaaaac tgattgatta
     4201 tgaaggtata tttgctggag gttcaacagg ttcgattatt gcagcgattg agcagttgat
     4261 aacgtcaatt gaagaaggtg caacaattgt cacgatttta ccagatcgag gcgatcgtta
     4321 cttagattta gtttattcag atacatggtt agaaaaaatg aaatcaagac aaggagttaa
     4381 atcagaatga atagagagat gttgtattta aatagatcag atattgaaca agcgggagga
     4441 aatcattcac aagtttatgt tgacgcatta acagaagcat taacagcgca tgcgcacaat
     4501 gattttgtac aaccgcttaa gccgtattta agacaggatc ctgaaaatgg acacatcgca
     4561 gatcgaatta ttgcaatgcc aagtcatatc ggtggtgaac acgcaatttc aggtattaag
     4621 tggataggta gtaagcacga caatccatcg aaacgtaata tggagcgtgc aagtggtgtc
     4681 attattttga atgatccaga aacgaattat ccaattgcag ttatggaagc aagtttgatt
     4741 agtagtatgc gtactgcagc agtttcagtg attgcagcta agcatttggc taaaaaagga
     4801 tttaaagact taacaatcat tggttgcggg ctaatcggag acaagcaatt acaaagtatg
     4861 ttagagcaat tcgatcatat tgaacgcgtt tttgtttacg atcaattctc tgaagcgtgt
     4921 gcacgctttg ttgatagatg gcaacaacag cgtccggaaa ttaattttat tgcgacagaa
     4981 aatgctaaag aagcagtatc aaatggtgaa gtagtcatta catgtaccgt aacggatcaa
     5041 ccatatattg aatatgattg gttacaaaag ggtgcattta ttagcaacat ttctatcatg
     5101 gatgtgcata aagaagtctt tattaaagct gacaaagtcg tagtagatga ctggtcacaa
     5161 tgtaatcgag aaaagaaaac tattaaccaa ttggtgttag aaggtaaatt cagcaaagaa
     5221 gcacttcatg ctgaactagg gcaacttgtg acaggtgaca caccaggacg tgaagacgat
     5281 gatgaaatca tattacttaa tccgatgggt atggctatcg aagatatttc aagtgcttac
     5341 tttatttatc aacaggcaca acaacaaaat attgggacaa cattgaacct atattaagaa
     5401 tgcgaggtgt ctgaacattg cagaatcata cagcagtcaa tacagcacaa acgataatat
     5461 taagggattt agttgatgca ttattatttg aagatatagc cggtattgta tcgaatagtg
     5521 agattactaa agaaaatgga caaaccattt tgatatacaa acgtgaaaca caacaaataa
     5581 agatacctgt ttattttagt gctttaaata tgtttcgtta tgaaagttca caaccaatta
     5641 cgatagaggg aagggcgtct aagcaacctt taacggcagc tgaattttgg caaacaatcg
     5701 ctaatatgaa ttgtgattta agtcatgaat gggaagtggc tcgcgttgaa gaaggactga
     5761 ctactgctgc cacacagctt gctaaacaat tatcagaatt agatttagcg tcgcatcctt
     5821 ttgtgatgtc cgaacagttt gcaagtttaa aagatcgtcc atttcatcca ttagctaaag
     5881 aaaaaagagg attaagagaa gcggattatc aagtgtatca agctgaatta aatcaatcat
     5941 ttcctttaat ggttgcagca gttaaaaaga cacaaatgat tcatggcgat actgcaaata
     6001 ttgatgaatt agaatcattg acagcaccta taaaagaaca agcgacagat atgttacatg
     6061 atcaagggtt atcaatagat gactatgtac tatttcctgt acatccttgg caatatcaac
     6121 atattttgcc gaacgtcttt gcgaaagaga ttagggaaaa attggttgta ccattaccat
     6181 taaaatttgg agattatcta tcggcttcaa gtatgcgttc attaattaat atcgcagcgc
     6241 cgtataacca tgttaaagtg ccatttgcaa tgcagtcatt aggcgcatta aggctaacgc
     6301 ctacgcgtta catgaaaaac ggagaacaag ccgaacgatt attacgtcaa cttatagaaa
     6361 aagatgccat gttagctaag tatgtcacag tttgtgatga aacagcttgg tggtcatata
     6421 tgggtcaaga taatgatatt ttcaaagatc aattaggtca tttaactgtt cagctaagaa
     6481 aatatcccga agtgctagcc aaaaatgata cgcaacagct agtgtcaatg gcagcactcg
     6541 cggcaaatga tcgcacctta tatcaaatga tttgtggaaa agataatatt tctaaaaaag
     6601 atgtcatgac gttatttgaa gatatcgcac aagtcttttt aaaggtaaca ctatcattta
     6661 tgcaatacgg cgcattacca gagttgcatg gtcaaaatat attgttgtca tttgaagatg
     6721 gacgtgtaca aaaatgcgtg ttacgtgatc atgatactgt cagaatttat aaaccatggc
     6781 taacagcaca tcagctttca ttgccgaagt atgtcgtcag agaagataca cctaatacgc
     6841 taattaatga agatttggaa acattctttg cttattttca aacattagct gtatcggtaa
     6901 atctatatgc cattattgat gcaattcaag atttatttgg tgtaagtgag catgaactta
     6961 tgtcgttgtt aaaacaaatt ttaaaaaatg aagtggcaac tatttcctgg gttacaactg
     7021 atcagctagc tgtcagacat attttatttg ataaacagac gtggccattt aaacaaattt
     7081 tattaccatt gctatatcaa cgtgatagtg gtggaggtag tatgccttca ggtttaacta
     7141 ccgtaccaaa tccaatggtg acatatgatt aatcagtcta tatggcgcag taactttcgc
     7201 attttatggc tcagtcagtt tatagcgatt gctggactga cagtacttgt gccattattg
     7261 ccaatttata tggcatcact acaaaatcta tcagtcgtag aaatacagtt gtggagtggt
     7321 atagcgattg ctgctccagc tgtaacgacg atgatagctt cgccgatatg ggggaagcta
     7381 ggtgataaga tcagtcgaaa atggatggtg ctacgagcgt tacttggttt ggcggtatgt
     7441 ttatttttaa tggcattgtg tactacacca ttacagtttg tacttgtgag gttattgcag
     7501 ggactatttg gtggtgttgt tgatgcatca agtgcgtttg cgagtgcaga ggcgccagct
     7561 gaagatcgtg gaaaggtatt aggaagactg caaagttcag tcagcgcagg gtctcttgtg
     7621 ggaccattaa ttggcggtgt tacagcttcg atattaggtt ttagtgcgtt actgatgagt
     7681 attgccgtta ttacttttat tgtctgtatt ttcggtgcat taaaattgat tgaaacgaca
     7741 catatgccaa aatcacaaac accaaatatt aataaaggta ttcgccgttc atttcaatgt
     7801 ctattatgca cacaacaaac atgtcgattt attatcgttg gcgttttagc aaactttgct
     7861 atgtatggca tgctaactgc attatcacca cttgcttcat cagtgaatca tacagcgata
     7921 gatgaccgta gtgtgattgg atttttacag tccgcatttt ggacggcttc gatattaagc
     7981 gcgcctttat ggggtcgctt taatgataaa tcatatgtta aatcagtata tatatttgcc
     8041 acgattgcat gtggttgtag tgcgatactg caaggtttag cgacgaatgt agaattttta
     8101 atggctgcaa gaatacttca aggattaaca tatagtgcat tgattcaaag tgtcatgttt
     8161 gtcgtcgtga atgcgtgtca tcaacaactt aaaggcacat ttgttggtac gacaaatagt
     8221 atgttagtta ttggtcaaat tattggtagt cttagtggcg ctgccattac aagttatact
     8281 acaccagcta ctacgtttat cgttatgggc atagtatttg ctgtaagtag tttattttta
     8341 atttgttcaa ccatcactaa tcaaatcaac gatcacacat taatgaaatt atgggagttg
     8401 aaacaaaaaa gtgcaaaata aagaattaat acaacatgca gcgcatgcgg ctatcgaacg
     8461 tattttaaat gaatatttta gagaagaaaa tttatatcaa gtaccacctc aaaatcatca
     8521 atggtctata caattatctg agctcgaaac gttaacaggt caattcgctt attggtctgc
     8581 gatggggcat catatgtatc atccagaagt atggcttatt gatggtaaaa gtaagaaatt
     8641 aacaacatat aaagaagcca ttgcgcgtat tttgcaacat atggctcaaa gtgcagataa
     8701 tcaaacggca gtgcaacaac atatggcgca aattatgtct gacatcgata atagcattca
     8761 tcgcacggcg cgttatttgc aaagtaacac aatagactac gcagaggatc gctatatcgt
     8821 ttcagaacaa tctttatatt taggtcatcc atttcatccg actcctaaga gtgcaagtgg
     8881 gttttcagaa gcagatttag agaaatatgc acccgaatgt catacatcat tccaattgca
     8941 ttatttagct gtgcatcaag atgttctgct cacgcgctat gtagaaggta aagaagatca
     9001 ggttgagaaa gtgttgtatc aattagcaga tatagacata tcagagatac ccaaagattt
     9061 tattttatta ccaatacatc cttatcaaat caatgtgttg cgacagcatc cacagtatat
     9121 gcaatatagt gaacaaggtt tgataaaaga ccttggcgtt tccggtgatt cagtgtaccc
     9181 gacgtcttcg gttagaactg tattttcaaa agcattaaac atttatttga aattaccgat
     9241 acacgttaaa atcactaatt ttatacgtac gaatgacctt gaacagattg aacggacaat
     9301 tgatgccgcg caagttatcg catcagtcaa agatgaggtt gaaacacccc attttaaatt
     9361 gatgtttgaa gaaggatatc gtgcattgtt accgaatcca ttagggcaaa cagttgaacc
     9421 tgaaatggat ttattaacaa atagtgccat gattgttcgt gaagggatac cgaattacca
     9481 tgctgataaa gatattcatg tattggcgtc attatttgaa acgatgcctg attcaccgac
     9541 ctctaagtta tcacaagtga ttgagcaaag tggtttagca ccagaagcat ggcttgaatg
     9601 ttatttggat cgtacattat tgccgatatt aaagctgttt agtaacacag gcattagtct
     9661 agaagcacat gtacaaaata cattaattga attaaaagat ggcataccag aagtgtgcta
     9721 tgtcagagat ttagaaggta tttgtctatc tagaacgatt gctactgaaa aacagcttgt
     9781 gccaaatgtt gttgcagcat ctagccctgt tgtatatgca catgatgaag cgtggcatcg
     9841 tcttaaatat tacgttgtag taaatcactt aggacattta gtatcaacta ttggtaaagc
     9901 gactagaaat gaagttgtgt tatggaaact tgtagcgcat cgtcttatga cttggaaaaa
     9961 agaatatgcg aataacgcag tatttattga ctgtgtagaa gatttatatc aaacgccgac
    10021 cattgcggct aaagcgaatt tgatgagtaa attgaatgat tgtggtgcaa accctattta
    10081 tacacatata ccaaatccaa tttgtcataa caaggaggta tcgtattgtg aatcaaacaa
    10141 ttcttaatcg tgtaaagact agagtgatgt accaattggt atcatcgctt atttatgaga
    10201 atattgttgt gtataaagcg tcatatcaag atggtgtcgg ttattttaca atagaaggaa
    10261 atgattcaga gtatcgtttt actgctgaaa agacacatag ctttgatcgt atacgtatca
    10321 catcaccaat tgagcgtgtc gtaggagatg aggcagatac aacaacagac tatacacaat
    10381 tactgagaga ggttgtattt acatttccta aaaatgatga aaagctagaa caatttattg
    10441 tcgagttatt acagacagaa ctaaaagata cgcaaagtat gcagtatcga gaatcaaacc
    10501 caccagcaac acctgagaca tttaacgact atgaatttta tgcgatggaa gggcatcagt
    10561 atcatccaag ttacaaatca cgtttaggat ttacgttgag tgataatttg aaatttggtc
    10621 ctgactttgt accaaacgtt aaactgcagt ggttagctat cgacaaagat aaagtagaaa
    10681 cgacggtatc aagaaatgtt gtagttaacg aaatgttacg tcaacaagtt ggcgataaga
    10741 cttatgaaca ttttgtacag caaattgaag cgtctggcaa acatgtaaat gatgttgaga
    10801 tgatacctgt acacccatgg cagtttgaac atgtcattca agttgatttg gctgaagaaa
    10861 ggcttaatgg cacagtacta tggttagggg aaagtgatga gctatatcac cctcaacaat
    10921 cgattcgtac gatgtcgcca atagacacga caaaatatta tttaaaggta ccaataagta
    10981 taacgaatac ttcaacgaaa cgagtgttgg cacctcatac aattgaaaat gcagcgcaaa
    11041 ttacggattg gttaaagcag atacagcaac aagatacgta tttaaaagat gaattaaaga
    11101 cagcttttct aggggaagtc ttaggacagt cttatttaaa tacacaactt tcgccttata
    11161 aacaaactca agtttatggt gcgttaggtg ttatatggcg tgaaaatata tatcatatgt
    11221 taatcgatga agaggatgcg ataccattta atgcacttta tgcgagtgat aaggatggtg
    11281 taccattcat tgaaaattgg attaaacaat atggttctga agcttggaca aagcaatttt
    11341 tagctgtagc gattcgtcca atgattcata tgctttatta tcacggtatt gcctttgaat
    11401 cgcatgcaca aaatatgatg ctcattcatg aaaatggttg gcctacacgt attgccttaa
    11461 aagatttcca tgatggtgtt cgttttaagc gtgagcattt aagtgaagct gcttcacacc
    11521 tgacattaaa gccaatgcca gaagcacata aaaaagtgaa tagtaattca tttattgaaa
    11581 cagatgatga acgtttagta cgtgactttt tacatgatgc atttttcttt attaatatcg
    11641 ccgaaatcat cttatttatt gaaaagcaat atggtatcga tgagaagctg caatggaaat
    11701 gggttaaagg catcattgag gcgtatcaag aagcatttcc agagctgaat aactatcaac
    11761 attttgattt gtttgaacct acgattcaag ttgaaaagtt aacgacacgt cgattattaa
    11821 gtgactccga gttaagaatt catcatgtta caaatccatt aggtgtagga ggtattactg
    11881 atgcaacaac tatctctgaa acatagatta aacaatggtg attcagttta tggcattttt
    11941 aattctatac cggacccatt gatgatagag gttatcgcag caagcgggta tgactttgtt
    12001 gtgattgata cagaacacgt ggcgattaat gatgagacac tagcgcattt aattcgtgca
    12061 gctgaagcag cgcatattat accaattgta cgtgtcactg cagtgataga tagagatatc
    12121 attaaagtgt tggatatggg tgcgagaggt attattgtgc cacacgttaa agatcgtgag
    12181 acagttgagc atattgtgaa attaagtcgt tattacccgc aaggattaag aagtttgaat
    12241 ggtggtcgca tggcaagatt tggacgtaca ccattacttg atgcaatgga gatggctaat
    12301 gagcatatta tggtgattgc catgatagaa gatgttgaag gggttatggc cattgacgat
    12361 atagcacaag tcgaaggttt agacatgata gtcgaaggtg ccgcagattt atcccagtca
    12421 cttggcatac catggcaaac gcgtgatgat caagtaacat cacatgttca acatattttt
    12481 gaagttgtga atgcacatgg taaacatttt tgtgcattac cacgtgaaga tgaagatatt
    12541 gcaaaatggc aggcacaagg tgtacaaaca tttattttag gtgatgatcg cggaaaaata
    12601 tatcgccatt taagtgcatc tctagcgacg tctaaacaga agggggatga agactaatgc
    12661 gtatagttca acctgttatt gaacagttaa aagcacaatc tcatccagtt tgtcattata
    12721 tctatgattt agtcggactg gaacatcatt tgcaacatat tacatcgtca ttgcctagta
    12781 attgtcagat gtactatgca atgaaagcaa atagtgaacg aacaatccta gatacaatta
    12841 gtcagtatgt tgaaggattc gaagttgcat ctcaaggtga aatagcaaaa ggtcttgttt
    12901 ttaaaccagc aaatcatatt atttttggtg gccctggtaa gacagacgag gaactaagat
    12961 atgcagtaag tgaaggtgtt cagcgtattc atgttgaaag tatgcatgaa ttacaacggc
    13021 taaatgccat cttagaagat gaagataaga cacaacacat tttattgcgt gttaatttag
    13081 caggaccatt tcccaatgca acgttgcata tggcaggacg cccaacacaa tttggtattt
    13141 ctgaagacga agttgatgat gtcattgaag ctgcgctagc aatgccaaat attcatctag
    13201 atggatttca ttttcattct atttctaaca atttagactc gaatttacat gtcgatgtag
    13261 tgaaacttta ttttaaaaaa gcaaaggcat ggtctgaaaa acatcaattt cctctcaaac
    13321 atatcaatct tggtggtggc ataggcgtta actatgcaga tttaactagc caatttgagt
    13381 gggataattt tgtagaaaat tttaaaacac ttatcgttga gcaagaaatg gaagatgtga
    13441 cattgaactt tgaatgtggg cgctttattg tggcacatat tggttactat gtgacagaag
    13501 tgctagacat taagaaagta catggtgctt ggtatgccat tttaagagga ggtacgcaac
    13561 aatttagact gccggtatct tggcagcata accatccttt tgacatttat cgctataagg
    13621 acaatccata ttcatttgaa aaagtttcaa tttcgagaca ggacacaacg ttagtcggtc
    13681 aattatgtac accgaaagat gtctttgcta gagaaataca gatagaggca atcagtacag
    13741 gcgacgttat tgttttcaaa tatgcaggtg catacggatg gtctatttca catcacgatt
    13801 tcttaagcca tccacatcct gaatttattt atttaacgca aacaaaggag gatgaataac
    13861 tattgaatca tattcatgaa catttaaaat tggtaccagt agataagatt gatcttcacg
    13921 aaacattcga acctttaaga ttggaaaaaa cgaaaagtag tattgaagca gatgatttta
    13981 tacgtcatcc tattttagtg acagcgatgc aacatggtag atatatggtt atagatggtg
    14041 tgcatcggta tacaagtttg aaagcgttag gatgtaagaa agttccagtg caagaaatcc
    14101 atgaaacaca atattcaatt agtacatggc aacataaagt tccatttggt gtgtggtggg
    14161 aaacgttaca acaagaacat cgcttaccat ggactactga gacaagacaa gaagcgccat
    14221 ttattacgat gtgtcatggt gatacagaac aatatttgta tacaaaagat ttaggcgaag
    14281 cacattttca agtatgggaa aaggttgtcg caagttatag tggttgttgt tctgtagaga
    14341 gaattgcaca aggtacatat ccttgtcttt ctcaacaaga tgtactcatg aagtatcagc
    14401 cattgagtta taaggaaatt gaagcggttg ttcataaagg ggaaactgtg ccagcaggtg
    14461 tgacacgctt taatatttca ggacgatgtc ttaatcttca agtaccactg gcattactta
    14521 aacaagatga tgatgttgaa caactgcgca attggaagca gtttttagca gataagtttg
    14581 ccaatatgag atgttatact gaaaaagtat acttggtgga gcaatagttt tactgtgatg
    14641 ttgagggaaa tatgatgatt tagcctatta atagcgaaaa tataataaaa caatatagtg
    14701 tggagaactt ttgatatttt ataaatattg aagttctcca tttttgtatt ttgcatataa
    14761 aaattaaata aaataaggta tattaaggta aagtataaat attaaataaa tggggaatag
    14821 acatggaaaa aaatgtagaa aaatcattca taaagatagg tttatatttt caaatagctt
    14881 atatagtact catggctata actttatgtg ggtttgtaat ttgctatgga ctaattttcg
    14941 gccttttcta tttattgtca ggtagcagag ctgattattt aatagtaacg atagttatat
    15001 cggcaataat ttctatattt gtaattatac tttcaatcgt acctgtcatc gtattggcat
    15061 ctgacttatt taaagaaagg atttcaaatg gtgtcatatt aattgtattg gctattatcg
    15121 ctttagtatt atgcaacttt gtatctgcaa tactctggtt tgtttcagcc atatctattt
    15181 taggtagaaa aaaattagta tctacatcag atactaccac tattcaaaaa agtaaaggga
    15241 acgcaaatca agcatcacat aaagacacgt gtaaaaagga acttgatagt caagacatga
    15301 tggaacatcc tgagtttaaa aatcccacga ctaaaaacct agaaggatta aacgaagaag
    15361 tacataaaga tgaagctaca actaaagttg acagtgataa tacggaaccg ccgattgaat
    15421 caaaagacca tgtctcgaaa aaagattgat gacaaactaa tcgagagact taaaaaataa
    15481 tattcaacat aaacactttt aaaacgacat ttaaacgcat tgccaatcac taaaggtagt
    15541 gcgtttaact ataccttaaa tatctgaata ttttgttaaa tggagttacc tttgttgtac
    15601 tattcaaatg aagaggagta aaatgtaatt aaaggaaagt aatttgagga gtgatcttta
    15661 tgacaaacaa caaagtagca ttagtaactg gcggagcaca agggattggt tttaaaattg
    15721 cagaacgttt agtggaagat ggtttcaaag tagcagttgt tgatttcaat gaagaagggg
    15781 caaaagcagc tgcacttaaa ttatcaagtg atggtacaaa agctattgct atcaaagcag
    15841 atgtatcaaa ccgtgatgat gtatttaatg cagtaagaca aactgcagcg caatttggcg
    15901 atttccatgt catggttaac aatgccggcc ttggaccaac aacaccaatc gatacaatta
    15961 ctgaagaaca gtttaaaaca gtatatggcg tgaacgttgc aggtgtgcta tggggtattc
    16021 aagccgcaca tgaacaattt aaaaaattca atcatggcgg taaaattatc aatgcaacat
    16081 ctcaagcagg cgttgagggt aacccaggct tgtctttata ttgcagtaca aaattcgcag
    16141 tgcgaggttt aacacaagta gccgcacaag atttagcgtc tgaaggtatt actgtgaatg
    16201 ccttcgcacc tggtatcgtt caaacaccaa tgatggaaag tatcgcagtg gcaacagccg
    16261 aagaagcggg taaacctgaa gcatggggtt gggaacaatt tacaagtcag attgctttgg
    16321 gcagagtttc acaacccgaa gatgtttcaa atgtagtgag cttcttagct ggtaaagact
    16381 ctgattacat tactggacaa acaattattg tagatggtgg tatgagattc cgttaataat
    16441 catccattaa taataaataa atccttattg ttaagtttaa tcacttagca gtaaggattt
    16501 tttagtgcac ttagaaggga ttgtattagt agaaaattaa taacagaagc tcttaagtga
    16561 gttatgatgt cacagtctaa tgcatcagtt gaaagcatta ctaaattcta tcaagcatag
    16621 tta
//
LOCUS       DATTQF010000048        16370 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.048, whole genome shotgun sequence.
ACCESSION   DATTQF010000048 DATTQF010000000
VERSION     DATTQF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.048"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            136..1047
                     /gene="rapZ"
                     /locus_tag="VB241_001316"
     CDS             136..1047
                     /gene="rapZ"
                     /locus_tag="VB241_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP1244616.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKKSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1044..2039
                     /locus_tag="VB241_001317"
     CDS             1044..2039
                     /locus_tag="VB241_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP1244617.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2148..3092
                     /gene="whiA"
                     /locus_tag="VB241_001318"
     CDS             2148..3092
                     /gene="whiA"
                     /locus_tag="VB241_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP1244618.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3661..4248
                     /gene="clpP"
                     /locus_tag="VB241_001319"
     CDS             3661..4248
                     /gene="clpP"
                     /locus_tag="VB241_001319"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEP1244619.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            4430..6064
                     /locus_tag="VB241_001320"
     CDS             4430..6064
                     /locus_tag="VB241_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEP1244620.1"
                     /translation="MKGIELLNNPFLNKGTAFTNEERKQLGLEGLLPANVRTLEQQAE
                     QCYEQFKAKQTDFEKRLFLMAIFNRNRTLFYKLTSEHLVEFMPIIYDPVIAQSIEQYN
                     ENFSRPQDAVFLSVDRQDQIEQELRNVANGRDIRLIVVTDAEGILGIGDWGVDGVDIA
                     IGKLIVYTAAAGINPAQVLPVSLDVGTNNEQLLNDELYLGNRHKRVDDETYYTFVDKF
                     VSAVRKEYPNALLHWEDFGRGHAKNILDKYEDTLPTFNDDIQGTGIVTLAGVLGALNI
                     SKVDYTNQTFLVYGGGTAGMGITNILKDELIKQDVSEDKANQHFYIMDKQGLLFDDMD
                     DLTEAQQVFAKNRNEFSNTVNWKNLKEVIENIKPTVLIGTSTQTGAFTEDAVKEMLKH
                     TERPMIFPLSNPTKLAEATAQDLLKWTNGKALIGTGIPYNDIEYNGVNYSIGQANNAL
                     MYPGLGLGLIASKAKKVNQQILSAASHALGGMVNPDEPGAAVLPPVEKIHQCSRAIAK
                     AVAQSVIDQGLNAETIDDIDEAIEAEIWYPEYKSYI"
     gene            complement(6271..7173)
                     /locus_tag="VB241_001321"
     CDS             complement(6271..7173)
                     /locus_tag="VB241_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEP1244621.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNNKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWVPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLISK"
     gene            7876..8505
                     /locus_tag="VB241_001322"
     CDS             7876..8505
                     /locus_tag="VB241_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HEP1244622.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            8999..10012
                     /locus_tag="VB241_001323"
     CDS             8999..10012
                     /locus_tag="VB241_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP1244623.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKDYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            10065..11075
                     /gene="gap"
                     /locus_tag="VB241_001324"
     CDS             10065..11075
                     /gene="gap"
                     /locus_tag="VB241_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP1244624.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            11214..12404
                     /locus_tag="VB241_001325"
     CDS             11214..12404
                     /locus_tag="VB241_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP1244625.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            12526..13287
                     /locus_tag="VB241_001326"
     CDS             12526..13287
                     /locus_tag="VB241_001326"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP1244626.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            13290..14807
                     /locus_tag="VB241_001327"
     CDS             13290..14807
                     /locus_tag="VB241_001327"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEP1244627.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVEDQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            14937..16241
                     /gene="eno"
                     /locus_tag="VB241_001328"
     CDS             14937..16241
                     /gene="eno"
                     /locus_tag="VB241_001328"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP1244628.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT         5975 a         2382 c         3116 g         4897 t
ORIGIN      
        1 ctccttttaa atgatgtata tttttagaaa tttaatctag acatagttta aaataaatat
       61 aaaacatcgt tgcttaattt tgtcatagaa catttaaatt aacatcatga aattcgtttt
      121 ggcggtgaaa aaataatgga taataatgaa aaagaaaaaa gtaaaagtga attattagtt
      181 gtcacaggtt tatctggcgc aggtaaatct ttggttattc aatgtttaga agacatggga
      241 tatttttgtg tagataatct accaccagtg ttattgccta aatttgtaga gttgatggaa
      301 caaggaaatc catccttaag aaaagtggca attgcaattg atttaagagg taaggaacta
      361 tttaattcat tagttgcagt agtggataaa gtcaaaagtg aaagtgacgt catcattgat
      421 gttatgtttt tagaagctag tactgaaaaa ttaatttcaa gatataagga aacgcgtcgt
      481 gcacatcctt tgatggaaca aggtaaaaaa tcgttaatca atgcaattaa tgatgagcga
      541 gagcatttgt ctcaaattag aagtatagct aattttgtta tagatactac aaagttatca
      601 cctaaagaat taaaagaacg cattcgtcga tactatgaag atgaagagtt tgaaactttt
      661 acaattaatg tcacaagttt cggttttaaa catgggattc agatggatgc agatttagta
      721 tttgatgtac gatttttacc aaatccatat tatgtagtag atttaagacc tttaacagga
      781 ttagataaag acgtttataa ttatgttatg aaatggaaag agacagagat tttctttgaa
      841 aaattaactg atttgttaga ttttatgata cctgggtata aaaaagaagg gaaatctcaa
      901 ttagtaattg ccatcggttg tacgggtgga caacatagat ctgtagcatt agcagaacga
      961 ctaggtaatt atctaaatga agtatttgaa tataatgttt atgtgcatca tagggacgca
     1021 catattgaaa gtggcgagaa aaaatgagac aaataaaagt tgtacttatc ggtggtggca
     1081 ctggcttatc agttatggct aggggattaa gagaattccc aattgatatt acagcgattg
     1141 taacagttgc tgataatggt gggagtacag ggaaaatcag agatgaaatg gatataccag
     1201 caccaggaga catcagaaat gtgattgcag ctttaagtga ttctgagtca gttttaagcc
     1261 aactttttca gtatcgtttt gaagaaaatc aaattagcgg tcactcatta ggtaatttat
     1321 taatcgcagg tatgactaat attacgaatg atttcggaca tgccattaaa gcattaagta
     1381 aaattttaaa tattaaaggt agagtcattc catctacaaa tacaagtgtg caattaaatg
     1441 ctgttatgga agatggagaa attgtttttg gagaaacaaa tattcctaaa aaacataaaa
     1501 aaattgatcg tgtgttttta gaacctaacg atgtgcaacc aatggaagaa gcaatcgatg
     1561 ctttaaggga agcagattta atcgttcttg gaccagggtc attatatacg agcgttattt
     1621 ctaacttatg tgtcaatggt atttcagatg cattaattca ttctgatgcg cctaagctat
     1681 atgtatctaa tgtgatgacg caacctgggg aaacagatgg ttatagcgtg aaagatcata
     1741 tcgacgcgat tcatagacaa gccggacaac cgtttattga ttatgtcatt tgtagtacac
     1801 aaactttcaa tgctcaagtt ttgaaaaaat atgaagaaaa acattctaaa ccagttgaag
     1861 ttaataaggc tgaactagaa aaagaaagca taaatgtaaa aacatcttct aatttagttg
     1921 aaatttctga aaatcattta gtaagacata atactaaagt gttatcgaca atgatttatg
     1981 acatagcttt agaattaatt agtactattc ctttcgtacc aagtgataaa cgtaaataat
     2041 atagaacgta atcatattat gatatgataa tagagctgta taaaaatgaa aatagacagt
     2101 ggttctaagg tgaatcatgt tttaaataag aaaggaatga ctgtacgatg agctttgcat
     2161 cagaaatgaa aaatgaatta actagaatag acgtcgatga aatgaatgca aaagcagagc
     2221 tcagtgcact gattcgaatg aatggtgcac ttagtctttc aaatcaacaa tttgttataa
     2281 atgttcaaac ggaaaatgca acaacggcaa gacgtattta ttcgttgatt aaacgtgtct
     2341 ttaatgtgga agttgaaata ttagtccgta aaaaaatgaa acttaaaaaa aataatattt
     2401 atatttgtcg tacaaagatg aaagcgaaag aaattcttga tgaattagga attttaaaag
     2461 acggcatttt tacgcatgaa attgatcatt caatgattca agatgacgaa atgagacgta
     2521 gttacttgag aggagctttt ctggcaggtg gctcagtgaa taaccctgaa acatcttcat
     2581 accatttgga aattttttct caaaatgaga gtcatgcaga aggcttaacg aaactaatga
     2641 atagttatga gttgaatgcc aaacatttag agcgaaaaaa aggaagtatt acgtatttaa
     2701 aagaagcgga aaagatttcg gattttctta gtttgatagg tggctatcaa gcgttattaa
     2761 aatttgaaga cgtacgtatt gtaagagata tgcgtaattc tgttaaccga ctcgttaatt
     2821 gtgaaacggc aaatctaaat aaaacagtta gtgctgcgat gaaacaagtt gagagcatta
     2881 aattgattga taaagaaatt ggtattgaaa atttaccaga caggttgaga gagattgcta
     2941 gaattcgagt agaacatcaa gaaatttcgt tgaaagagct tggagaaatg gtatcaactg
     3001 gtccaatttc aaaatcaggt gtaaatcatc gattaagaaa acttaatgat ttagccgata
     3061 agattagaaa tggtgaacaa atagaattat aagtaagaag gtgtttttgg tattgaatta
     3121 tcaaaaatac cttttttatg attaaaaagt gtttaatgaa taatagtcga ctaaccatta
     3181 aattaagctg ggaagtttaa tgaaggaaat tcaataacca agcgttacct aattttctca
     3241 ttaagtagca tcatatatat caatttggtc ataagaaaat atgaatgtga taattaattg
     3301 agtccctgaa agtccctgat catagacaaa aaatcaacat aaaaagaatc ccgacattgc
     3361 gggattctct gtattgaaaa gtattgtatt ttattaaaat gcctccttga agggaatcga
     3421 acccctatct taagaaccgg aatcttacgt gttatccatt acaccacaag gagtaaatgt
     3481 gttaatcaaa tcactgtact atgagaagta aaataagtac aatctaaaat agatattatc
     3541 agtttacaaa ggaaagagac aagcgtcaaa caatgtaact atttaaagtc aaagtgtttg
     3601 accaaatttg acttaatatg taaaataatg agtaacagtt attacaagga ggaaatatag
     3661 atgaatttaa ttcctacagt tattgaaaca acaaatcgcg gtgaacgtgc atacgatata
     3721 tactcacgtt tattaaaaga ccgtattatt atgttaggtt cacaaattga tgacaacgta
     3781 gcaaattcaa tcgtatcaca gttattattc ttacaagcac aagactcaga gaaagatatt
     3841 tatttataca ttaattcacc cggtggaagt gtaacagctg gttttgcgat ttatgataca
     3901 attcaacaca ttaaacctga tgttcaaaca atttgtatcg gtatggctgc atcaatggga
     3961 tcattcttat tagcagctgg tgctaaaggt aaacgttttg cgttaccaaa tgcagaagta
     4021 atgattcacc aaccattagg tggtgctcaa ggacaagcaa ctgaaattga aattgctgca
     4081 aatcacattt taaaaacacg tgaaaaatta aatcgcattt tatcagagcg tactggtcaa
     4141 agtattgaaa aaatacaaaa agacacagat cgtgataact tcttaactgc agaagaagct
     4201 aaagaatatg gcttaattga tgaagtgatg gtacctgaaa caaaataatt caaagtaaag
     4261 agtagactaa gcagtctgct ctttttgtat gattaaacca atttataaat aaatagttca
     4321 aaatattttg agtaaaagca aaaaataacg ggtaaaaata tttataagaa tttaattaga
     4381 cacgttacgt ctgcggttct acaatctaaa aaatataaag gatgatttta tgaaaggcat
     4441 tgaattactt aacaatccat ttttaaacaa aggtacagct tttactaacg aagaaagaaa
     4501 acagttaggg cttgaaggat tattacctgc gaatgttcga actttagaac aacaagcaga
     4561 acaatgttat gaacaattca aggcgaaaca aaccgatttt gagaaacgtt tatttttaat
     4621 ggcaattttt aatcgtaatc gtacattgtt ttacaaattg acttctgagc atttagttga
     4681 atttatgcca attatttatg accctgttat tgcacaatcg attgaacaat ataatgagaa
     4741 tttcagtaga ccccaagatg cagtattttt atctgttgat agacaagacc aaattgaaca
     4801 agaattgcgc aacgttgcaa atggcagaga tattcgatta atcgtagtaa cagatgcgga
     4861 aggtatatta ggtatcggcg attggggcgt tgatggtgta gatattgcga taggaaaatt
     4921 aatagtatat acagcggcag ctggaataaa tcccgcacaa gtattacctg tatctttaga
     4981 tgtaggtact aataatgaac aattactgaa tgatgaactt tatttaggaa atcgtcataa
     5041 acgtgtcgat gatgagacat attacacatt tgtagataaa tttgtgagtg cagtgcgtaa
     5101 agaatatcct aatgcactat tacattggga agattttggt agagggcatg ctaaaaatat
     5161 tttagataaa tatgaagata ctttaccaac atttaatgat gatattcaag gtacaggtat
     5221 agttacatta gccggcgtac taggtgcatt aaatatttct aaagtagact atacgaatca
     5281 gacgttctta gtttatggtg gtggtacagc tggtatgggt attaccaata ttttaaaaga
     5341 tgaactaatt aaacaggatg tttcagaaga taaagcaaat cagcatttct atattatgga
     5401 taaacaagga ttgttgtttg atgatatgga tgacttaact gaagctcaac aagtatttgc
     5461 taaaaatagg aatgaatttt caaatacagt aaactggaaa aatttaaaag aagtaattga
     5521 aaatattaag cctactgttt taatcggtac atcaactcaa acaggtgcat ttacagaaga
     5581 tgcagtaaaa gagatgttaa aacatacaga acgtccaatg attttcccat tgtctaatcc
     5641 tacaaaatta gcagaagcaa ctgcacaaga tttattaaaa tggactaatg gaaaagcatt
     5701 aattggaact ggtatcccat ataatgacat tgaatacaat ggtgtgaatt acagtattgg
     5761 tcaagcaaat aatgcattga tgtatcctgg tttaggtttg ggtcttattg cgtcaaaagc
     5821 gaagaaagta aatcaacaga ttctatcagc tgcgagtcat gcacttggtg gcatggtcaa
     5881 tccagatgaa ccaggagctg cagtcttacc acctgttgag aaaatacatc aatgttctcg
     5941 tgccatagcc aaagcggttg cacaaagtgt cattgatcaa ggattaaacg ctgaaactat
     6001 tgatgatatt gatgaagcaa ttgaagctga aatttggtat cctgaataca aatcatacat
     6061 ttaattttat aaagtagtaa aaggtcattt gaacattttg tttcaagtgg ccttttcgta
     6121 tttactttaa aattaatcaa ccaatattca taagtgactt taaagttata tctatatgta
     6181 atatcagcaa atagaaaagt ctagagagat ttaacgaatt atctcctaga ctttttgctt
     6241 gaagtggagt aggagagatc aatgtcccac tcacttacta attaaatctt caagtgccat
     6301 ttttaaatta ctatatttaa attggaatcc caatgcttga attttattag gtaatacttt
     6361 ttgagtatcc aatactactg ttgacatttg accaagtatg agacgcattg caagacttgg
     6421 tacccaagtt tcatgaggct tatgcatagc tcttgctaaa gtgtagccaa ataaattttg
     6481 acgctcaggt ataggtgcag ttaaattaaa cggaccacta gctgactcgt tatttattaa
     6541 aaataaaata gcttgaatta aatcattgat atgaatccat gaataccatt gttgaccaga
     6601 acctaattta ccaccaatgt aatattcgta tggtagtttc attgtttgta acgcaccgcc
     6661 ttcattcgat aaaattatac cgaaacgacc gatgacaact cgtgtaccta attgttcaaa
     6721 ttgttgtgcg aaacgttccc attgatacac aatatctgat aagaaatcaa atggtaaagt
     6781 tttataaact tctgtgtaac tcataaataa atcaggagga tagtaaccag tggcactagc
     6841 attaaataaa actttaggtg ctttattacg tgatttaaac aattcatata aagcttgcgt
     6901 agattgaatt ctacttagca ttagcgtttg tttatattcc ggtgtccatc gtttattcaa
     6961 tgtagcacct gctaagttga tgaccacatc gatattttga ggaactttgt gttcccaccc
     7021 agatttagcc cagttgacat atgaaatttt cttattattt gaaatttggt cgtgtcgcgt
     7081 taatatcgtg atatgtgaat ctgatttttt gatttcatta actaattgag atccaaccat
     7141 accagtccca ccagtaatta agtattgttt cattatcatt caccccatgt aattttgtat
     7201 ttagttacta tttaataatc attattgttg ttcaaaggtt atacattatt tagacaataa
     7261 tatgtcaata actttttttg aattgtattt taataaaaat gatataagtt ataataaagg
     7321 tgtaccttcg ataacgaata aacatctctt aaaagtatgt gtaaaacgct gcatgataca
     7381 aacgaaggta aaaatttgac tccctttagt agtggacccg tacgtttatc gtgcgggtcg
     7441 tttttattta tatcttattc ccgttataca tcaattcaaa gcattaattt tttaaacaaa
     7501 tttaagaata gatagtaata taacaatcta aacataaaaa cttttaacac aacacttaaa
     7561 ctaatgcttt aattttcaat acgtagctat aatttgttgt aaaatcaaaa aggttaatat
     7621 gttaattttc aaaaaaaggc tcaaaatatg tttgatttag ttattaaatg ttaagatata
     7681 taagactact atttctttgt aaaaatgaat ccgatttacg agtgagtaat agtgaatata
     7741 gttttaagtt gaagaaggca aaaagagtaa atgtttatta atgtttgtag aaacaaggta
     7801 agcaaattag ttgtgaaaat gttaatagtt acgtgataat ttctatattt aaattagttt
     7861 gaagtgaggg agagtatgtc gaatcaaaat tacgactaca ataaaaatga agatggaagt
     7921 aagaagaaaa tgagtacaac agcgaaagta gttagcattg cgacggtatt gctattactc
     7981 ggaggattag tatttgcaat ttttgcatat gtagatcatt cgaataaagc taaagaacgt
     8041 atgttgaacg aacaaaagca ggaacaaaaa gaaaagcgtc aaaaagaaaa tgcagaaaaa
     8101 gagagaaaga aaaagcaaca agaggaaaaa gagcagaatg agctagattc acaagcaaac
     8161 caatatcagc aattgccaca gcagaatcaa tatcaatatg tgccacctca gcaacaagca
     8221 cctacaaagc aacgtcctgc taaagaagag aatgatgata aagcatcaaa ggatgagtcg
     8281 aaagataagg atgacaaagc atctcaagat aaatcagatg ataatcagaa gaaaactgat
     8341 gataataaac aaccagctca gcctaaacca cagccgcaac aaccaacacc aaagccaaat
     8401 aataatcaac aaaacaatca atcaaatcaa caagcgaaac cacaagcacc acaacaaaat
     8461 agccaatcaa caacaaataa acaaaataat gctaatgata agtagtattt agtcaaacaa
     8521 aaatgaacca gtatgacaga caacacaatt aattaggttg tctcgaatat tggttcttat
     8581 ttttataatt gttaattagg ggagagatga tacttaaata gttagttgtt tattttgcag
     8641 atagtgaaat ttattttttc agtcaactac tggcaatgta acttttcaga ttctagagca
     8701 ttgatttatg tcctagtctc aaataataag caaatgatta gagaatcagt ataagggtat
     8761 acatttgact acagcgaata aaataaaacg ttttgttgta taataaatcg aaaatatatt
     8821 gcaagcgctt tatcaaatta tttagaaaat ttaagtttta tgcttgcaat ttttgaaata
     8881 gaatagtact attgcaagtg taaagaggtt aatttttgtc ccacgtggga cttaaaaagg
     8941 caaccactgg ttgtgacata tccttattta catttataaa tataaggagg aggtagtagt
     9001 gaaagactta ttgcaagcac agcaaaagct tataccggat ctcatagata aaatgtataa
     9061 acgtttttct attcttacta ctatctcaaa aaatcagcct gtcggacgtc gaagtttaag
     9121 cgaacatatg gatatgactg aacgtgtact gcgttctgaa acagatatgc ttaagaaaca
     9181 agatttgata aaagttaagc ctaccggaat ggaaattaca gctgaaggtg agcaactgat
     9241 ttcgcaattg aaagattact ttgatatcta tgcagatgac aatcgtctgt cagaaggtat
     9301 taagaataaa tttcaaatta aggaagttca tgttgttcct ggtgatgctg ataatagcca
     9361 atctgttaaa acagaattag gtaggcaagc aggtcaatta cttgaaggca tattacaaga
     9421 agatgcgata gttgctgtaa ctggcggatc cacgatggca tgtgttagtg aagcaattca
     9481 tttattacca tataatgtat tcttcgtacc agccagaggt ggactaggcg aaaatgttgt
     9541 ctttcaggca aacacaattg cagccagtat ggcacaacaa gctggcggtt attatacgac
     9601 gatgtatgta cctgataatg tcagcgaaac aacatataac acattgttgt tagagccatc
     9661 agtcataaac actttagaca aaattaaaca agcaaacgtt atattacacg gcattggtga
     9721 tgcgctgaag atggcgcatc gacgtcaatc acctgaaaag gtcattgaac aacttcaaca
     9781 tcatcaagct gtcggagagg catttggtta ttattttgat acacaaggtc aaattgtcca
     9841 taaggttaaa acaattggac ttcaattaga agaccttgaa tcaaaagact ttatttttgc
     9901 agttgcagga ggcaaatcga aaggtgaagc aattaaagca tacttgacga ttgcacccaa
     9961 gaatacagtg ttaatcactg atgaagccgc agcaaagata atacttgaat aagagataaa
    10021 aagtttaata ctttttaaat atcattttaa aggaggccat tataatggca gtaaaagtag
    10081 caattaatgg ttttggtaga attggtcgtt tagcattcag aagaattcaa gaagtagaag
    10141 gtcttgaagt tgtagcagta aacgacttaa cagatgacga catgttagcg catttattaa
    10201 aatatgacac tatgcaaggt cgtttcacag gtgaagtaga ggtagttgat ggtggtttcc
    10261 gcgtaaatgg taaagaagtt aaatcattca gtgaaccaga tgcaagcaaa ttaccttgga
    10321 aagacttaaa tatcgatgta gtattagaat gtactggttt ctacactgat aaagataaag
    10381 cacaagctca tattgaagca ggcgctaaaa aagtattaat ctcagcacca gctactggtg
    10441 acttaaaaac aatcgtattc aacactaacc accaagagtt agatggttct gaaacagttg
    10501 tttcaggtgc ttcatgtact acaaactcat tagcaccagt tgctaaagtt ttaaacgatg
    10561 actttggttt agttgaaggt ttaatgacta caattcacgc ttacacaggt gatcaaaata
    10621 cacaagacgc acctcacaga aaaggtgaca aacgtcgtgc tcgtgcagca gcagaaaaca
    10681 tcatccctaa ctcaacaggt gctgctaaag ctatcggtaa agttattcct gaaatcgatg
    10741 gtaaattaga tggtggtgca caacgtgttc ctgtagctac aggttcatta actgaattaa
    10801 cagtagtatt agaaaaacaa gacgtaacag ttgaacaagt taacgaagct atgaaaaatg
    10861 cttcaaacga atcattcggt tacactgaag acgaaatcgt ttcttcagac gttgtaggta
    10921 tgacttacgg ttcattattc gacgctacac aaactcgtgt aatgtcagtt ggtgaccgtc
    10981 aattagttaa agttgcagct tggtatgata acgaaatgtc atatactgca caattagttc
    11041 gtacattagc atacttagct gaactttcta aataatttta gtatagtttt tattcaaata
    11101 cgctagtgct cagaactatt tagcattaat taaagcttat gagtaagcgg ggagcacaaa
    11161 cgcttctccg cttattttta tataaaattt cctaattaca aggaggaaac accatggcta
    11221 aaaaaattgt ttctgattta gatcttaaag gtaaaacagt cctagtacgt gctgatttta
    11281 acgtaccttt aaaagacggt gaaattacta atgacaaccg tatcgttcaa gctttaccta
    11341 caattcaata catcatcgaa caaggtggta aaatcgtact attttcacat ttaggtaaag
    11401 taaaagaaga aagtgataaa gcaaaattaa ctttacgtcc agttgctgaa gacttatcta
    11461 agaaattaga taaagaagtt gttttcgtac cagaaacacg cggcgaaaaa cttgaagcag
    11521 ctattaaaga ccttaaagaa ggcgacgtat tattagttga aaatacacgt tatgaagatt
    11581 tagacggtaa aaaagaatct aaaaatgatc cagaattagg taaatactgg gcatctttag
    11641 gtgatgtgtt tgtaaatgat gcttttggta ctgcgcatcg tgagcatgca tctaatgttg
    11701 gtatttctac acacttagaa actgcagctg gattcttaat ggataaagaa attaagttta
    11761 tcggtggcgt agttaacgat ccacataaac cagttgttgc tattttaggt ggagcaaaag
    11821 tatctgacaa aattaatgtc atcaaaaact tagttaacat cgctgataaa attatcatcg
    11881 gcggaggtat ggcttatact ttcttaaaag cgcaaggtaa agaaattggt atttcattat
    11941 tagaagaaga taaaatcgac ttcgcaaaag atttattaga aaaacatggt gataaaattg
    12001 tattaccagt agacactaaa gttgctaaag aattttctaa tgatgccaaa attactgtag
    12061 taccatctga ttcaattcca gcagaccaag aaggtatgga tattggacca aacactgtaa
    12121 aattatttgc agatgaatta gaaggtgcgc acactgttgt atggaatgga cctatgggtg
    12181 tattcgagtt cagtaacttt gcacaaggta caattggtgt atgtaaagca attgcaaacc
    12241 ttaaagatgc aattacgatt atcggtggcg gtgattcagc tgcagcagca atctctttag
    12301 gttttgaaaa tgacttcact catatttcaa ctggtggcgg agcgtcatta gagtacctag
    12361 aaggtaaaga attgcctggt atcaaagcaa tcaataataa ataataaagt gatagtttaa
    12421 agtgatgtgg catgtttgtt taacattgtt acgggaaaac agtcataaga tgacatcgtg
    12481 tttcatcact tttcaaaaat atttacaaaa caaggagtgt ctttaatgag aacaccaatt
    12541 atagctggta actggaaaat gaacaaaaca gtacaagaag caaaagactt cgtcaatgca
    12601 ttgccaacat taccagattc aaaagaagta gaatcagtaa tttgtgcacc agcaattcaa
    12661 ttagatgcat taactactgc agttaaagaa ggaaaagcac aaggtttaga aatcggtgct
    12721 caaaatacgt atttcgaaga taacggtgcg ttcacaggtg aaacgtctcc agttgcatta
    12781 gcagatttag gcgttaaata cgttgttatc ggtcattctg aacgtcgtga attattccac
    12841 gaaacagatg aagaaattaa caaaaaagcg cacgctattt tcaaacatgg aatgactcca
    12901 attatttgtg ttggtgaaac agacgaagag cgtgaaagtg gtaaagctaa cgatgttgta
    12961 ggtgagcaag ttaagaaagc tgttgcaggt ttatctgaag atcaacttaa atcagttgta
    13021 attgcttatg aaccaatctg ggcaatcgga actggtaaat catcaacatc tgaagatgcg
    13081 aatgaaatgt gtgcatttgt acgtcaaact attgctgact tatcaagcaa agaagtatca
    13141 gaagcaactc gtattcaata tggtggtagt gttaaaccta acaacattaa agaatacatg
    13201 gcacaaactg atattgatgg ggcattagta ggtggcgcat cacttaaagt tgaagatttc
    13261 gtacaattgt tagaaggtgc aaaataatca tggctaagaa accaactgcg ttaattattt
    13321 tagatggttt tgcgaaccgc gaaagcgaac atggtaatgc ggtaaaatta gcaaacaagc
    13381 ctaattttga tcgttattac aacaaatatc caacgactca aatcgaagcg agtggcttag
    13441 atgttggact acctgaagga caaatgggta actcagaagt tggtcatatg aatatcggtg
    13501 caggacgtat cgtttatcaa agtttaactc gaatcaataa atcaattgaa gacggtgatt
    13561 tctttgaaaa tgatgtttta aataatgcaa ttgcacacgt gaattcacat gattcagcgt
    13621 tacacatctt tggtttattg tctgacggtg gtgtacacag tcattacaaa catttatttg
    13681 ctttgttaga acttgctaaa aaacaaggtg ttgaaaaagt ttacgtacac gcatttttag
    13741 atggtcgtga cgtagatcaa aaatccgctt tgaaatacat cgaagagact gaagctaaat
    13801 tcaatgaatt aggtattggt caatttgcat ctgtgtctgg ccgttattat gcaatggacc
    13861 gtgacaaacg ttgggaacgt gaagaaaaag cttacaatgc tattcgtaac tttgatgccc
    13921 caacttatgc aactgccaaa gaaggtgtgg aagcaagtta taatgaaggc ttaactgacg
    13981 aattcgtagt accattcatc gttgaggatc aaaatgacgg tgttaatgat ggagatgcag
    14041 tgatcttcta taatttccga cctgatagag cggcacaatt atcggaaatt tttgcgaaca
    14101 gagcattcga aggatttaaa gttgaacaag ttaaagactt attctatgca acattcacta
    14161 agtataatga caatatcgat gcggctatcg tcttcgaaaa agtcgattta aataatacaa
    14221 tcggtgaaat tgcacaaaat aacaatttaa ctcaattacg tattgcagaa actgaaaaat
    14281 atcctcacgt tacttacttt atgagtggtg gacgtaatga ggaatttaaa ggtgaacgtc
    14341 gtcgtttgat tgattcacct aaagttgcaa cgtatgactt gaaaccagaa atgagtgctt
    14401 atgaagttaa agatgcatta ttagaagagt taaataaagg tgacttggac ttaattattt
    14461 taaactttgc taaccctgat atggttggac atagtggtat gcttgagccg acaatcaaag
    14521 caatcgaagc ggttgatgaa tgtttaggcg aagtggttga taagatttta gacatggacg
    14581 gttatgcaat tattactgct gaccatggta actctgatca agtattgacg gatgatgatc
    14641 aaccaatgac gacgcataca acgaacccag taccagtgat tgtaacaaaa gaaggcgtta
    14701 cacttagaga aactggtcgt ttaggtgact tagcacctac attattagat ttattaaatg
    14761 tagaacaacc tgaagatatg acaggtgaat ctttaattaa acactaatat tgtaaaagat
    14821 gttaagtaaa cgcttaatga cacttatttt ttgaaaataa tagtaatatc attttgttaa
    14881 atgaaagaat aaagctataa taattataga ataactattt aaaggagatt ataaacatgc
    14941 caattattac agatgtttac gctcgcgaag tcttagactc tcgtggtaac ccaactgttg
    15001 aagtagaagt attaactgaa agtggcgcat ttggtcgtgc attagtacca tcaggtgctt
    15061 caactggtga acacgaagct gttgaattac gtgatggaga taaatcacgt tatttaggta
    15121 aaggtgttac taaagcagtt gaaaacgtta atgaaatcat cgcaccagaa attattgaag
    15181 gtgaattttc agtattagat caagtatcta ttgataaaat gatgatcgca ttagacggta
    15241 ctccaaacaa aggtaaatta ggtgcaaatg ctattttagg tgtatctatt gcagtagcac
    15301 gtgcagcagc tgacttatta ggtcaaccac tttacaaata tttaggtgga tttaatggta
    15361 agcagttacc agtaccaatg atgaacatcg ttaatggtgg ttctcactca gatgctccaa
    15421 ttgcattcca agaattcatg attttacctg taggtgctac aacgttcaaa gaatcattac
    15481 gttggggtac tgaaattttc cacaacttaa aatcaatttt aagcaaacgt ggtttagaaa
    15541 ctgcagtagg tgacgaaggt ggtttcgctc ctaaatttga aggtactgaa gatgctgttg
    15601 aaacaattat ccaagcaatc gaagcagctg gttacaaacc aggtgaagaa gtattcttag
    15661 gatttgactg tgcatcatca gaattctatg aaaatggtgt atatgactac agtaagttcg
    15721 aaggcgaaca cggtgcaaaa cgtacagctg cagaacaagt tgactactta gaacaattag
    15781 tagacaaata tcctatcatt acaattgaag acggtatgga cgaaaacgac tgggatggtt
    15841 ggaaacaact tacagaacgt atcggtgacc gtgtacaatt agtaggtgac gatttattcg
    15901 taacaaacac tgaaatttta gcaaaaggta ttgaaaacgg aattggtaac tcaatcttaa
    15961 ttaaagttaa ccaaatcggt acattaactg aaacatttga tgcaatcgaa atggctcaaa
    16021 aagctggtta cacagcagta gtttctcacc gttcaggtga aacagaagat acaacaattg
    16081 ctgatatcgc tgttgctaca aacgctggtc aaattaaaac tggttcatta tcacgtactg
    16141 accgtattgc taaatacaat caattattac gtatcgaaga tgaattattt gaaactgcta
    16201 aatatgacgg tatcaaatca ttctataact tagataaata atcattttaa acattaagta
    16261 ctgccataat tttagttgag gattattatg tcggtataaa ttgaataaag tttttgagtt
    16321 cacgcttaaa aagttcacgc ttttatttat aacctgccac agagttgaga
//
LOCUS       DATTQF010000049        16274 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.049, whole genome shotgun sequence.
ACCESSION   DATTQF010000049 DATTQF010000000
VERSION     DATTQF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.049"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            157..1779
                     /locus_tag="VB241_001329"
     CDS             157..1779
                     /locus_tag="VB241_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEP1244629.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMVVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(1949..2512)
                     /locus_tag="VB241_001330"
     CDS             complement(1949..2512)
                     /locus_tag="VB241_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEP1244630.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAHKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQSSDSKN"
     gene            complement(2601..2696)
                     /locus_tag="VB241_001331"
     CDS             complement(2601..2696)
                     /locus_tag="VB241_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244631.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            2765..4255
                     /gene="betB"
                     /locus_tag="VB241_001332"
     CDS             2765..4255
                     /gene="betB"
                     /locus_tag="VB241_001332"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEP1244632.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEIDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            4516..6225
                     /gene="betA"
                     /locus_tag="VB241_001333"
     CDS             4516..6225
                     /gene="betA"
                     /locus_tag="VB241_001333"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEP1244633.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGHRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            6503..6715
                     /locus_tag="VB241_001334"
     CDS             6503..6715
                     /locus_tag="VB241_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HEP1244634.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            6995..7438
                     /locus_tag="VB241_001335"
     CDS             6995..7438
                     /locus_tag="VB241_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEP1244635.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDTL
                     CVLNHLYINEGQEETFEQKFLQRNKHLQNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADML"
     gene            7632..9230
                     /locus_tag="VB241_001336"
     CDS             7632..9230
                     /locus_tag="VB241_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP1244636.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPDSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPEHTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(9290..9621)
                     /locus_tag="VB241_001337"
                     /pseudo
     CDS             complement(9290..9621)
                     /locus_tag="VB241_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9917..11413
                     /gene="mqo"
                     /locus_tag="VB241_001338"
     CDS             9917..11413
                     /gene="mqo"
                     /locus_tag="VB241_001338"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEP1244637.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(11606..12496)
                     /locus_tag="VB241_001339"
     CDS             complement(11606..12496)
                     /locus_tag="VB241_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEP1244638.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     ANLYKDLAEHPNVVRIVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(12619..13035)
                     /locus_tag="VB241_001340"
     CDS             complement(12619..13035)
                     /locus_tag="VB241_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244639.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDIISNQSHLKALLLNLDF
                     LIPSDNTPYTLEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLVFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            13289..14626
                     /locus_tag="VB241_001341"
     CDS             13289..14626
                     /locus_tag="VB241_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEP1244640.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALENICRENGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKNLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(14706..16154)
                     /locus_tag="VB241_001342"
     CDS             complement(14706..16154)
                     /locus_tag="VB241_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1244641.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSIAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
BASE COUNT         5618 a         2579 c         3061 g         5016 t
ORIGIN      
        1 tatataaata ttcaataatt gccaactttc aaattgtatg aaatttgcat tattattaaa
       61 tattagttat tgtcaatttt gtgaatcaat ataattatta cattttgaga taaatcgaaa
      121 caggattcat aaaattaata attaggggga gcacaattga aaaaagagaa agttatggac
      181 tggacgacct ttatagggac agtagctgta cttctttttg cagttatacc tatgatggct
      241 tttccaaaag caagtgaaga tatcatcact ggtattaata gtgccatttc tgattcaatt
      301 ggttcgatat atttatttat ggggctggcg attttttgct ttgtaatgta tatcgcattt
      361 ggtaaatatg gtaatgtcac acttggtaaa gcaagtgata aaccagaatt taatacattt
      421 acatgggcgg caatgctgtt ttgtgcaggc ataggctctg atattttata ctggggcgtt
      481 attgaatggg ctttttacta tcaagttcca ccaaatggcg cgaaaagtat gagtgatgaa
      541 gcactccaat atgcgacgca atatggtatg ttccactggg gaccaattgc ttgggctatt
      601 tacgttttac cagcattacc aattggttat ttagtgtttg ttaaaaaaca accggtgtat
      661 aaaattagtc aagcttgtcg cccgatttta aaaggtcaaa cagataaatt tgtaggtaaa
      721 gttgtagata tcttatttat ctttggattg ctaggtggtg cggcaacatc actagcgtta
      781 ggtgtgccat taatttctgc aggcatagaa agattaactg gtttagatgg taaaaatatg
      841 attttacgtt cggccatttt attaacaatc acggttatat ttgccattag ttcatataca
      901 ggattgaaaa aaggtattca aaagttaagt gatatcaacg tttggctatc atttgtactt
      961 ttagccttta tatttattat tggaccgact gtttttatta tggaaacgac agtgacaggg
     1021 ttcggaaata tgttgagaga tttcttccat atggcaacat ggttagaacc attcggtggt
     1081 attaaaggtc gaaaagaaac gaatttccca caagactgga caatattcta ctggtcatgg
     1141 tggttagtat atgcgccatt tatcggttta tttatcgcta gaatttcaaa aggtcgacgc
     1201 cttaaagaag tcgtgctagg aacaattatt tatggaacgc ttggatgcgt attattcttt
     1261 ggtatttttg gtaactatgc tgtgtattta caaatttctg gacagtttaa tgtaacacaa
     1321 tatttaaata cacatggtac agaggcaacc attattgaag tggtgcatca tttaccattc
     1381 ccatcattga tggttgtact attcttagta tctgctttct tattcttagc aacaacattt
     1441 gattcgggtt catatatttt agcggcagca tctcagaaaa aagtggtagg cgaaccatta
     1501 cgtgccaatc gtttattctg ggcgtttgca ttgtgcttat tgccattttc attgatgcta
     1561 gttggtggtg aacgtgcatt agaagtattg aaaactgcat caatactggc aagtgtgcca
     1621 ttaattgtta tttttatttt catgatgata tcatttttaa tcattttagg acgcgataga
     1681 attaaacttg aaacgcgggc tgaaaaatta aaagaagttg aacgtcgctc attgcgaatc
     1741 gttcaagtat cagaagaaga acaagacgat aatttataat tcaattaatg atgcataaga
     1801 ttttgaattt tataagtata tgattaatcg tgatattaag caaaaacggt aatttctatt
     1861 tattgagata gaaattaccg tttatttcgt ggacctattg cattgttttc atcatgcata
     1921 atcatcattg tcgttgtttg agtcaatttt aatttttaga atcagaagac tgttctggaa
     1981 ttgggaaata tttaaaaatt tcaccgcttt caatcgcttc ggttaactgt tctaaccatt
     2041 cgtaataaac atgtgtatga tcaagctgag ctttaatttt gtgtgcctct tgtgtttcag
     2101 cttcagttaa atcactgctt tcaagtaatg gattgataat agcttgagca tcttttactg
     2161 cttcgacatt gatatcaatt tcacgctgaa attttttagt gaaaaagttt cggaaaaaga
     2221 tgaaaaagtc tttctcggcg ataaaatgtt gtttgcggct tcctctcgta aattgttgtt
     2281 taacaatatc aaattcctgc aatttcttaa cgccagcact catacttggt ttgctcattt
     2341 gcaattgatg acgcatttca tcaagcgtca tactgccttc aaacaccata gtgccatata
     2401 agtttcctac acttctatta gtgccataca aatccattgt ctgtccaatt gaattaatta
     2461 caatatcttt tgcttgttct aattgttgct gtttgttctg agaacgagtc atcattgcac
     2521 ctccgtacat cattttggtc acgttaaaat aaatactaat acattataaa accttttcta
     2581 aaaaaagaca ttaaaaatat ttaaagcatt aaagttaaat gtttcgttaa ataaaaatct
     2641 aacgaactta caaaacttaa ttcttgagtt gttttgtaaa ttgacacatt tttcatttct
     2701 atgctaacat aagtttgtaa aattcgttaa ataaaaattt aacaaactta acggaggttg
     2761 ttgaatggaa cttttaaaac atttatctca gcgtcaatat attgatggtg agtgggttga
     2821 aagcgcgaat aaaaatacaa gagatattat caatccttac aatcaagaag tgatatttac
     2881 ggtttctgaa gggacaaaag aggatgcaga acgtgcaatc ttagctgcaa gacgtgcgtt
     2941 tgagtctggt gaatggtcgc aagaaactgc tgaaacaaga ggtaaaaaag tacgtgccat
     3001 cgcggacaag atcaaagaac atcgcgaagc gttagcacga ttagaaacat tagatactgg
     3061 aaaaacgtta gaagaatcat atgcagatat ggatgatatt cataatgtgt ttatgtattt
     3121 tgctggatta gcagataaag atggtggcga aatgattgat tcaccaatac cagatacaga
     3181 aagcaaaatt gttaaagaac cagtaggtgt agttacacaa attacacctt ggaattatcc
     3241 gttattacaa gcatcatgga aaattgcgcc agcgcttgct acgggttgtt cactagttat
     3301 gaaaccaagt gaaattacac cattaacaac aatacgtgtt tttgaattaa tggaagaagt
     3361 tggtttccct aaaggaacaa ttaatcttat tcttggtgca ggttctgaag ttggtgacgt
     3421 aatgtcaggt cataaagaga ttgacctagt atcatttaca ggtggcattg agactggtaa
     3481 gcatattatg aaaaatgctg ctaataatgt tacgaatatt gccttggaac ttggcggtaa
     3541 aaatccaaac attatctttg atgatgctga ttttgaattg gcggtagacc aagcgttaaa
     3601 tggtggatat ttccatgcag gtcaagtttg ttcagcagga tcaagaatat tagtacaaaa
     3661 cagtattaaa gacaaatttg agcaagcact tattgatcgc gtgaaaaaaa tcaaattagg
     3721 taatggtttt gatgctgata ctgaaatggg accagtgatt tcaacagaac atcgtaataa
     3781 aatcgaatct tatatggatg tagctaaagc agaaggcgca acaattgctg ttggtggtaa
     3841 acgcccagat agagacgatt taaaagatgg tctattcttc gagccaacag tcattacaaa
     3901 ttgtgatacg tcaatgcgta ttgtacaaga agaggttttc ggacctgtcg ttactgtaga
     3961 aggctttgaa actgaacaag aagcgattca attagcgaat gattctatat atggtttagc
     4021 aggtgctgta ttttctaaag atattggaaa agcacaacgc gttgctaaca agttgaaact
     4081 tggaacggtg tggattaatg atttccaccc atattttgca caagcgccat ggggtggata
     4141 caaacaatca ggtatcggta gagaattagg caaagaaggc ttagaagagt accttgtttc
     4201 aaaacacatt ttaacaaata caaatccaca attagtgaat tggtttagca aataaaaatt
     4261 agataaggtg agtgccattg taagaacaca agacactcac tttgttttgt ataagtggcg
     4321 aaatgttgat tgataatttg gactaaacgc aaaatgaatc atagattatt tcatgactgt
     4381 tagtaacaat cataaaagga aaatcgagtg ttttggttag ctaagtttag caattcaacg
     4441 ataaccaatc agccactaac aaatatttca tgcaatactc actttgaaat acaacaaact
     4501 ttggaggtca taacgatgag taacaaaaat aaatcatatg attatgtcat cattggagga
     4561 ggcagtgcag gttctgtact aggtcatcgt ctgagtgaag ataaagataa agaagtctta
     4621 gtattagaag cgggtcgcag tgattatttt tgggatttat ttatccaaat gcctgctgcg
     4681 ttaatgttcc cttcaggcaa taaattttac gattggattt attcaacaga tgaagaacca
     4741 catatgggcg gtcgtaaagt agcacatgcc cgaggtaaag ttttaggtgg atcaagttcg
     4801 attaatggca tgatttatca acgcggtaat ccaatggact atgaaggctg ggcagaacca
     4861 gaaggtatgg aaacttggga ttttgcgcac tgtttaccgt attttaaaaa attagaaaaa
     4921 acatacggtg cagcgcctta tgataaattt agaggccatg atggaccaat taagttaaaa
     4981 cgagggccag caacgaatcc tttattccag tcattctttg atgcaggtgt tgaagcaggc
     5041 tatcataaaa cacctgatgt gaatggattt agacaagaag gttttggacc gttcgatagt
     5101 caagtacatc gtggtcgccg aatgtcagct tcaagagcat atttacatcc agctatgaag
     5161 cgtaaaaact taaccgttga aacacgtgcc tttgttactg aaattcatta tgaaggtaga
     5221 agagcaactg gtgttacgta taagaaaaat ggcaaactac ataccatcga tgctaatgaa
     5281 gtcatcttgt caggtggggc attcaatacg ccacaattac tacaactgtc tggtatcggc
     5341 gattcagaat tcctaaaatc aaaaggcatt gagccacgtg ttcatttgcc tggtgtgggt
     5401 gaaaactttg aagatcactt agaggtatac attcaacata agtgtaagga acctgtatca
     5461 ttacagccaa gcttagatat caagcgcatg ccatttattg gtttacaatg gattttcaca
     5521 cgtactggtg cagcagcatc taaccatttt gaaggtggcg gatttgtacg ttcaaataac
     5581 gaagttgatt atcctaactt aatgttccat ttcttaccaa tagctgtaag atatgatggt
     5641 caaaaggcag cggtcgcaca tggctatcaa gttcacgtag gaccaatgta ttcaaactct
     5701 cgtggcagct tgaaaatcaa atctaaagat ccattcgaaa aaccaagtat ccgctttaat
     5761 tatttatcaa cagaagaaga taaaaaagaa tgggtagaag caattcgtgt agcaagaaat
     5821 atcttatctc aaaaagcaat ggatccattc aatggtggag aaatttcacc aggaccagaa
     5881 gtgcaaacag acgaagaaat tcttgattgg gttcgtagag atggtgaaac agcattacac
     5941 ccatcttgta gtgctaaaat gggtcctgct tcagatccaa tggcagtagt tgacccgtta
     6001 acaatgaaag ttcatggtat ggaaaattta cgtgtagttg atgcatcagc tatgccacgt
     6061 acgacaaatg gcaatataca cgcacctgtt ttaatgttag ctgaaaaagc agcagacatt
     6121 atacgtggca gaaagccttt agagccacaa tatattgatt attataagca tggtgttcat
     6181 gatgaaaatg aaggtgcaat cgaagtaaaa ccatacgcta aataaattga tatagtagac
     6241 ttttagtctg gcatctattg tattggtgcc aggctttttt gcgttttatc taaatgagaa
     6301 gttaatgtgt tgttcaaaaa cggtgtgtga gaatatgaac tgaaaataga gttaattgta
     6361 cattgtgatg attaattgac ctaattgaca gacattaaaa gagtgtacaa ccttaaaaag
     6421 ttcactaaat agacatttaa ttgtgttttc ttgaaagctt tcgtggtagg atttatataa
     6481 gtaaattgag agaaggaata ctatgagctt ttacgaatat atacaaacat ttaaagatga
     6541 taaaacacca ttaggcgaat tagcgatttg gattaaagaa gatgattcat tcccaaaaca
     6601 agagaagcta acagaaaaca ttttgtctta ttttcatcaa atgtccaata tagatcatga
     6661 gtttttagaa attgtaaaaa gatcactttc tctgtatgat caattaaaat cgtaaaatta
     6721 gagataaagt tagctaaaat acaaggccgg taatttcata gatgaagttg tcggctattt
     6781 tcatgactaa tgttaaataa tgatcatgga attgaagaca caaaaataat gggcgacgcc
     6841 tgtccccatt tgtatggaat attaatactt tttacaaagc cttatctttt aaaaagaagt
     6901 caaactatga ttgtaattaa taaatacggg aatgaattag taatagatat aaagtaatat
     6961 gaccaaaaat ataaatgaaa tgaaggcatt tattatgaac atttatgatg aatatagaag
     7021 ttatgtaata gaagaactgg atgattgtct cacaatacaa aaaaataatg acactgcata
     7081 ttacgatgtg ctagaagcga taaatgattt atcaaatgat acattatgtg tgttaaatca
     7141 tttatatatt aacgaaggac aagaagaaac attcgagcaa aaatttttgc aacgtaataa
     7201 gcatttgcaa aacgttgatg ggtttaaagc attaaggttt ttaagaccta gaacagctgg
     7261 tagacactat atcattatta cgctgtggga aaataggcaa gcattttatc attggcaaaa
     7321 ttcagctgag tacaagcata cacacaagca tcgtggcact tcgaaaggtg cagatgtaaa
     7381 aataattaat cgtgaattat catacaatat tcgtattgaa ttagctgata tgctctagcc
     7441 cgagatatca accctcaatc atttgaaagt ccttgaatgc tatgtcatgt caaagcgtag
     7501 tgtcgaggac tttggtattt aaccctattt aattgaaaat tctgtaaaga taggcgtatg
     7561 atatatataa ttcatttata aaattacaat gttcatcaca ggaaaagtgg atgtatgaaa
     7621 ggaagttagt tatgcaaaag tcagctctat tcgcgcctga aaaatataac attatttcgg
     7681 aaatcgaaaa atatagccac ataccagata aaaaggcaat tttatatcat aatactgaag
     7741 gcgaagatat atcagtcact taccaacagc tcattgaaca gtctaataaa gtaggtaatg
     7801 ttttagcatc gcacggttta tctaaaggcg ataaagtact cattatgatg ccacgatcta
     7861 ttgcgacata tgaattatat attgctgctt taaaattagg tgttgcaatc attccatgtt
     7921 ctgaaatgct aagaactaaa gatttacaat accgtatcac acacggtgaa ataaatgctg
     7981 ttattgcact agagccattc acagttgaat ttgaaaaaat taaagaatat gatgcgttaa
     8041 ctaaatttgt tattgccggt caaaaagatg gatggatttc acttgaagct gaaaaagaaa
     8101 acgcaagtaa caaactagaa atggcagata cgacgcgtga tgatatggcg attctgtcat
     8161 atacatctgg tacaactggc aatcctaaag ccgttacaca ttgtcatgga tggggatttg
     8221 cacatttaca aatggcacca aaacattggt tatgtattaa tgaagatgac ttagtttggg
     8281 caacagcagc accaggttgg caaaaatggg tatggagtcc tttcttatct gtattaggtt
     8341 caggtgcaac tgcttttgta tttaatggaa gattcagccc tgagacatac ttagaattgc
     8401 ttcaaaaata tcaaattaat gttttatgtt gtacaccgac agaatatcgt atgatggcta
     8461 aattacagaa cttaaatgat tatgacttaa cgcatttaca tagtgccgtt tctgcaggtg
     8521 agccactaaa tcgagaagta gttgaacaat tcaagaaata ctttaattta actgtaagag
     8581 atggatatgg ccagactgaa agtaccttgc ttataggctt tttaaaagat acaccacaaa
     8641 ggattggatc aatgggtaaa ggcattccgg atagttcggt cactgtagta gatgatgagg
     8701 ggaatagcgt acctgctaat acgaaaggga atattgcagt accacttgat ttgccagcac
     8761 ttttcaaagg atattataaa gagccagaac atacggcgaa agcgcaaaca ggtaaatact
     8821 acatcactgg tgacttagct catgtagatg atgatggtta tttctggttt gaaggacgtc
     8881 gagatgacat tattattagt tcaggctata cgattgggcc ttttgaagtt gaagatgcgt
     8941 taacgaatca tccagccgtt aaagaatgtg ctgtagtagc aaaacctcat gatatacgag
     9001 gaaatattgt taaagcattc gttattttac aggaccatac tgcgggtgat gatacgctag
     9061 ttaaagaact acaacaattt gttaaaaatg aagttgcacc atataaatat ccaagagaga
     9121 ttgaattcgt tgacgactta ccaaaaacaa actcaggtaa aattcgtcgt gttgaactac
     9181 gagatgctga aatagaaaaa tggcaacaac aaaaagatag caatcaataa acatagcaaa
     9241 gcagtccaat gcgtaaggta tgatgatacg cactggactg cttgtttatt tatgatgttc
     9301 tttgtcgtct agttataaat attaaaatta gtttagaaac aagcgcaacc agtattaaga
     9361 atgagaaaat ataagatgtt aaacctcgaa tatcccagtt taataaaaaa acattactat
     9421 caccggcatt aagttgaaac tcagtatgtg ttaaaacgat aatcacaatg cctataagtg
     9481 caagattcaa aataatttac aatattaaag gtaattgaag tgattttctt ggatgcatag
     9541 gcattgaatc tcctttttat gattgatttt acacttatat tgtatcaaaa tagagtatag
     9601 gtttaaaatg ctataagcca aatgtagatt aaaacaatcg ttagctattt gttgtcaaat
     9661 aattatgatt gatttgatat ttatcttcta tgaataaaag atttaatata ttgcaaagtt
     9721 aatgaataat tataatttac atttaccact gcttgcttta acatatacat gtaagcacta
     9781 actatagtat tttgttacag cgtatcaatt aaaacaggaa gttatctatg gcgttgatat
     9841 tttaaagaaa tatgtcagtt ttatgtaact ttcatattga tgatacacat ttacaatttt
     9901 tggagaggtg aaaccaatgg ctaagtctaa tagtaaagac atcgttttaa ttggagccgg
     9961 tgtacttagc acaacatttg gttcaatgtt aaaagaaatt gagccagact ggaatatcca
    10021 cgtttacgaa cgcttggatc gtcctgcaat cgaaagttca aacgaaagaa ataatgctgg
    10081 tacgggtcat gcagcattat gtgagttgaa ctacacagtt ttacaacctg atggttctat
    10141 cgacatcgaa aaagcgaaag tgattaacga agagtttgag atttcaaaac aattctgggg
    10201 tcacttagtg aaaagcggta gcatcgagaa cccaagagaa tttatcaatc cattaccaca
    10261 catcagttat gttagaggta aaaacaatgt taaattctta aaagatcgtt acgaagcgat
    10321 gaaagctttc cctatgttcg ataatatcga atatactgaa gacatcgaag taatgaaaaa
    10381 atggattcca ttgatgatga aaggccgtga agataaccct ggtatcatgg cggcaagtaa
    10441 aattgacgaa ggtacagatg taaacttcgg tgaattaaca cgtaaaatgg ctaaaagcat
    10501 tgaagcacat ccaaatgcta ctgtgcaatt taaccatgag gttgttgatt ttgaacaatt
    10561 gtcaaatggt caatgggaag ttactgttaa aaatcgccta actggtgaga aattcaaaca
    10621 agtaactgac tatgtattca tcggtgctgg cggtggagca attccattat tacaaaaaac
    10681 aggtatccct gaaagtaaac atttaggtgg attccctatc agtggtcaat tcttagcttg
    10741 tacaaaccca caagttattg aacaacacga tgccaaagtt tatggtaaag agccacctgg
    10801 tacaccacca atgactgtac ctcatttaga tacgcgttac attgatggtc aaagaacatt
    10861 attatttgga ccatttgcta atgttggacc taaattcttg aaaaatggtt ctaacttaga
    10921 tttattcaaa tcagttaaaa catacaacat tacaacttta ttagcagcag ctgttaaaaa
    10981 cttaccttta attaaatact catttgatca agtgattatg acaaaagaag gttgtatgaa
    11041 ccacttacgt actttctatc cagaagcacg taatgaagat tggcaattat acactgctgg
    11101 taaacgtgta caagttatca aagatacacc tgaacacggt aaaggattca tccaattcgg
    11161 tacagaagtg gttaactcac aagaccacac tgtaatcgca ttattaggtg aatcaccagg
    11221 ggcttcaact tcagtttcag ttgcgttaga agtattagaa cgtaacttcc ctgaatacaa
    11281 aactgaatgg gcacctaaaa tcaagaaaat gattccatca tacggtgaat cattaattga
    11341 agacgaaaaa ttaatgagaa aaatccgtaa acaaacttca aaagacttag aattaggtta
    11401 ctacgaaaac taaattctaa tagttatatt taaaatcgga caactgagaa atcttaaaga
    11461 tttttcagct gtccgatttt tttacgtaat ctaatacata cggcaaaatc aactttaatc
    11521 aaaaagactc atacacaatg cctttaaagc acatgtatga gtccttttta gtagtttata
    11581 tcaaaaaata cgtttaatgt ataaattagt ttttgtttac agatgcgtcg tagattgatt
    11641 ctacagcgtc acctaatgct ttatcgaatt cttctttaga ttgtccagct cttaaatcac
    11701 tagctaatgc gcgtgagaag cttgcgataa gttcagcgtt atctttaagt aattcattag
    11761 ctttttctct gctgtaacca cctgataata caacgatacg aacaacgttt ggatgctcag
    11821 ctaagtcttt gtataagtta gcttcagtag gaattgttaa ttttaacata actaattgat
    11881 cagcatttaa gctgtctaaa ccttttttaa gctcagcttt taatactttt tcaatttcag
    11941 ctttatcttt tgcgttaata ttaacttctg gttcgataat tggcactaac cctttagcaa
    12001 taatttgttt agcgacttca aattgttgtt caacaacgtc tttgatacct tgttcattta
    12061 attctaaaat attagaacgc atttttgtac caaaaatgtg acgctcgttt gcacgatcta
    12121 ataagctatc taagttgtcg attggtttca ttaattgaac accattttgc tcttcagcaa
    12181 gacctttgtc tacttttaag aacggaacaa cacctttgtc agctaagtaa tctgcagtgt
    12241 atttgccttc tacttcgcga tccattgttt gttcgaaaag aatagcacct aaaattttat
    12301 ctggtgagaa tgaaggtgaa gttaccacac gtgtacgcat atcgtgaaca agttggaaca
    12361 tttcgtcttc attgctatat tgatcttcgt ttacaccata ttctttaagt gcttttggtg
    12421 tactaccacc actttggtct aatgcggcaa taaagccttt tccatttttc attttttcta
    12481 attgctcttt attcatactt tccactcctt aacttttcaa tacacctcca gtatgataaa
    12541 aatgagaaca tttctcaagt cataaacctt gaaaagtgta taaaatgtga aaaataattg
    12601 tcagtttaat taaaaatatt attttattct aggtatgact aacgccatta atgacatgaa
    12661 gaaaatatgt gtaataatcc aaccgattaa ttctgtcaca ctaaattgaa aaattggacg
    12721 ttgcgcaata aatactaaaa agggatacaa tgctataaat aagaaaacta aagggatata
    12781 acatagatag taaagccttt tagaagtatg aaacagcaac acaaatacaa aataaatgac
    12841 gctaccaatt agtaagtgac atattatttc tagagtatat ggtgtattgt cagaaggaat
    12901 tagaaaatct aaattcaata acaaagcttt taaatgtgat tgattgctta ttatgtcaaa
    12961 caacatggtc atccatagca acatgaatga tatgaaaata gcagcaatga aatggaacaa
    13021 tacctttaaa cgcattcata tcacctcaga gtcaaatgga atatagtaaa caattcccta
    13081 atcgaacaaa attatgcgca taaataaagt agattgatat aaaattctta attatcagaa
    13141 tatatttaca aatctgaatt ttattagtat attggatagt attcataatg acatgacagt
    13201 attttcacgt gatatcaaat cgaatgtggt tatcgatgta gggaaggtaa ttatgtgaat
    13261 aaggtctgtc aatgggaggg gaatgtatat gagtaaagca catcaattaa ttcaagagga
    13321 tgaacattat tttgcgaaat caggacgtat taaatattat ccgttagtta ttgatcatgg
    13381 atatggagca acattggttg atattgaggg aaagacatat atcgatttgt tatcgagtgc
    13441 gagttctcaa aacgtaggtc atgcacctag agaagtaaca gaagcgataa aggcacaagt
    13501 tgataaattt attcattata caccagcata tatgtatcat gaaccgttgg tacgtttagc
    13561 taagaagctt tgtgagattg cacctggaga ttttgaaaaa agagtgacct tcggattaac
    13621 tggatcagac gcaaatgatg gcatcattaa atttgccaga gcatatacag ggcgtcctta
    13681 tatcattagt ttcactaatg catatcatgg ttcaactttt ggctcgttgt ctatgtcagc
    13741 tattagttta aatatgcgca aacattatgg tccgttattg aatggctttt atcatattcc
    13801 gtttccagat aaatatcgtg gtatgtacga gcagccacaa gctaattcag tagaagaata
    13861 tttagcaccc ttaaaagaaa tgtttgcgaa atatgtacct gctgacgaag tagcatgtat
    13921 tgttattgaa acgatacaag gcgatggtgg acttttagaa ccagttccag ggtattttga
    13981 agcgttagaa aatatttgtc gtgaaaatgg tattttaatc gctgtcgatg atattcaaca
    14041 aggttttggg agaacaggta catggagttc agtctcgcat tttaatttta cgcctgattt
    14101 aatcactttc ggaaaatctt tagcaggtgg tatgcctatg tcagcaattg ttggacgcaa
    14161 agagattatg aattgtttag aagcaccagc acatttattt acaacaggtg ctaatccagt
    14221 tagttgtgaa gctgcattag ccacaattca aatgattgaa gatcagtcgc ttcttcaggc
    14281 tagtgcggaa aaaggggaat atgttaggaa acgaatggat caatgggtat ctaaatacaa
    14341 tagtgtaggc gatgttagag gtaaaggcct gagcattggt attgatattg tttccgacaa
    14401 aaacctcaaa acacgtgatg ccagtgcggc acttaaaatt tgtaattact gctttgagca
    14461 tggcgtagtt attatagctg tagcaggaaa tgtgttgcga ttccaaccgc cattggtaat
    14521 aacatatgag caattagaca cggcgttaaa cactatagaa gatgcactga ctgctttgga
    14581 agcaggaaac ttagatcaat atgacatatc tggacaaggt tggtaatagc gattatctta
    14641 atataaaata aaaaatcatt tccacatctg gatgttaatc agatgggaaa tgattttttt
    14701 aatttttatt ttggtgggtg gtattcagct acgtcatttt tcttagaatg tctaagtcca
    14761 taacttaaat atagaatgat accaacaata aaccaaatta aagtgtataa tttcgcttcg
    14821 aatcctaatc cccagaatac tagcaatact aaaacaaatg taactgctgg taacacagga
    14881 tataaaggta atttaaacgc aggaattggt aaatctttac cttcacgctt tctcaaacga
    14941 tacattgcta atgaaacgaa cataaatgca acaagtgtac cggctgaaat taattgtgct
    15001 aaaaatgcga atgggaacat agaaccaatt aaaacaccaa taatagtaag tataactagt
    15061 gcacgattag gtaaatgttt gtcgtttaag tggcttaacc atgaaggtaa taagccgtca
    15121 cgtccaaatg aataaagtaa acgtgagcct gctaacatca taccaattaa tgctgtaaac
    15181 ataccgataa cagagatagc ttgaacaata gctgctacaa caccatgacc actttgacgt
    15241 aaagcccaac caacaggttc agcattgttt gcgtattgtg agtaatggaa cataccaact
    15301 aacacaagtg ctacagcaat aaataatacg atagcaatac ttaaagaacc aagaatacct
    15361 ctaggcattg tcttttgagg gtcaagtgct tctgctgagt ttgctgcgat agaatcgaaa
    15421 ccgatatacg ctaagaaaat cattgaaaca ccagcatata tgccttgcca tccaccaaag
    15481 tcacctgtag cagttacttt gtgttctgga ataaatggca catagttact aacatttatt
    15541 gctgttaaac ctacgattac aaataaaata atagctaata cttttaaaat aactaaaatg
    15601 ttttccatac gtgctgcttc cgacatacca cgtgatagta ataatgcagt taataaaata
    15661 acgatagcag caataatatc gataaaaccg ccatttgtac caaatggatt tgataatgct
    15721 gcaggtaatt cgatgccaat tggtttcaca agtccgcgta aattcgctga gaatcctgat
    15781 gcaacaaagg ctacggcgat aaaatattca gctaataaag cccaaccggc aacccatcca
    15841 aagaattcac caaataatac attgacccaa gaataggctg aacctgcaaa tggcatagcg
    15901 gcagccattt ctgcataagt aaatgcaact aaaccagcaa caatagcagc gagtaagaat
    15961 gataacgcaa cggccggtcc tgcatgttct gcagcaacaa tgccaggtag cgtaaagata
    16021 gatgtcgata caattgttcc tacacctaaa gctaagaaat cacgcacccg aagtgtacgc
    16081 tttaaatgac catctttatt ttgatagata gccggatcct cttttcgtgc tattttattg
    16141 aaaaaacttc ccataaactt tcctcccaaa ccttcataaa caattctata cggtgttttt
    16201 taatatgtta tatcatagca caaataatca atattttgtc taaaaattct gaaaaatcac
    16261 aactttatgt tact
//
LOCUS       DATTQF010000050        16040 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.050, whole genome shotgun sequence.
ACCESSION   DATTQF010000050 DATTQF010000000
VERSION     DATTQF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.050"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(340..801)
                     /locus_tag="VB241_001343"
     CDS             complement(340..801)
                     /locus_tag="VB241_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEP1244642.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREIDKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(826..1737)
                     /locus_tag="VB241_001344"
     CDS             complement(826..1737)
                     /locus_tag="VB241_001344"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEP1244643.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            2188..3099
                     /locus_tag="VB241_001345"
     CDS             2188..3099
                     /locus_tag="VB241_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEP1244644.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(3367..6819)
                     /locus_tag="VB241_001346"
     CDS             complement(3367..6819)
                     /locus_tag="VB241_001346"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEP1244645.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKAAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(7372..8598)
                     /gene="ftsW"
                     /locus_tag="VB241_001347"
     CDS             complement(7372..8598)
                     /gene="ftsW"
                     /locus_tag="VB241_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEP1244646.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(8912..9187)
                     /locus_tag="VB241_001348"
     CDS             complement(8912..9187)
                     /locus_tag="VB241_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEP1244647.1"
                     /translation="MAKQTTMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            9328..9810
                     /locus_tag="VB241_001349"
     CDS             9328..9810
                     /locus_tag="VB241_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244648.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            9812..9979
                     /locus_tag="VB241_001350"
     CDS             9812..9979
                     /locus_tag="VB241_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HEP1244649.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(10118..11965)
                     /gene="typA"
                     /locus_tag="VB241_001351"
     CDS             complement(10118..11965)
                     /gene="typA"
                     /locus_tag="VB241_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP1244650.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTLINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            12067..12258
                     /locus_tag="VB241_001352"
     CDS             12067..12258
                     /locus_tag="VB241_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HEP1244651.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(12412..13239)
                     /locus_tag="VB241_001353"
     CDS             complement(12412..13239)
                     /locus_tag="VB241_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP1244652.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPVLGEIFKDVVNISRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILNE
                     VDGLASNLLGEPLTISGPNSILVGNRGVHREILGVYLEPHRDALIQLHEQRFKRKSK"
     gene            13396..14010
                     /locus_tag="VB241_001354"
     CDS             13396..14010
                     /locus_tag="VB241_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HEP1244653.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            14194..15546
                     /locus_tag="VB241_001355"
     CDS             14194..15546
                     /locus_tag="VB241_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEP1244654.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQG
                     "
     gene            complement(15628..>16040)
                     /locus_tag="VB241_001356"
     CDS             complement(15628..>16040)
                     /locus_tag="VB241_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEP1244655.1"
                     /translation="KPDLIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAKQLS
                     QNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSLMAL
                     NLIAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         5303 a         2884 c         2340 g         5513 t
ORIGIN      
        1 ttaacttttt catcaatcaa ccacctaaaa agtattaata ctattgtaaa aaacaacaca
       61 ttaattagca aattttcaac actgacataa ctgtgtcatt tcgatgaaac aaaaccatct
      121 tcaggcacaa tgttaacatc ataaagattt tccctacagt ctatatcata acctatcttt
      181 atgttttcag gttgaatttc attattagga ttaaaatatg tataaaaaaa cggacagtta
      241 aggtatcaaa tgaataccat caactgtccg actattcttc atcaaaaaac ctgataaaac
      301 aaattgcctt atcagattag tatcatttgt aaaagcgtat taatgaccaa atgttgcttt
      361 aatcagtgat gtcgtttctc cacctggata taatacatat aataataagt aaactgctac
      421 acctgtaatt gcagtacaga accatataac tgaagcgaat ggcccgaatt tgcggtgcac
      481 attatattta tctttaaatg cagtaataat ttgaactaga cctagaatac caccaattgt
      541 tgctaaatta atgtggaaaa ataagaaaat tgtataatat ttcttaattg atgctggtcc
      601 gccaaatgct gtattaccga taaaaatcgt tctacttgca tagattaaga aaaaagttaa
      661 agcaaaaaca gctgcagcta acataacatt tttgtgttta tctatttccc ttttccaaat
      721 aagtctccaa ccaatggcaa ttaaaattgc actaatgaca atacatgtcg tacttatcgt
      781 tggtaaaatt ggaacgccca tatttttcat cctaacttaa ttaatctaga tcaaagtaag
      841 taatgaaaca atcacagcta acacgaaaaa gatcaccaaa taatttagtg aatatataaa
      901 catttgtgtt gcccattttg tttgatctga atttttctta aatgttgtta aacctaatgc
      961 aatccatcct aaatttaata atgtagctaa cactacgaat acgacaccta aatttattag
     1021 taataaaggc actggcaata aaataatcaa ccagataaac atactgacac gtgtacgttt
     1081 aaagccctta actgatggta acattggaat atttgcaagt gcatattcat ctttacgttt
     1141 aatagctaag gcataaaaat gaattggttg ccaacaaaat acaactaaaa acagcgcaat
     1201 cgctgttaaa ctaatttgtc cttcaattgc aacccatcca attagtggtg gtactgctcc
     1261 aggaaaactc ccaatcactg tgttccatgt tgtatgtctt ttagaccata ttgagtaata
     1321 agacacataa cctacaatcc ccataagacc aagtacgcct gatggtatat tcaataaaaa
     1381 caaacaaatt tctccaacta acatcatacc aaaacttaaa agtaataaat tttgatctgt
     1441 aattctatta tttacagttg gtctattttg tttactaggc ataatacgat caatatcttg
     1501 gtcgtaataa ttatttaacg cacatgcgcc acccataatt aaagtagatc ctaatagcat
     1561 taataaaatt tgaggtattg atgataagaa ggaatgattt gtcattacaa ctgctagcca
     1621 tgcgcccgca aaagccggaa ttaagttacc ttgaacaagt cccattttaa ttatctgttg
     1681 caattctttg aagttaactc tgctaatatt ttgtgacaaa gtatgctctt tgctcataat
     1741 ccccctcctt aaatttgttc atataagatt atgatatctt agattgcata aaaagactag
     1801 gtttaataaa attaaattgt gacaaattaa cgacaagaga aaatgtcaat tttgtgacac
     1861 aaataacatt taatttattg ctataatgta tatgttagaa aattttaata agtagaatca
     1921 tgcatctaaa agagattaat atttaagctt caaatttgag taactgtgac tgaaatttta
     1981 tagcagtgaa cggcacatca ttgtcgtgtt attcatatca ttgatgatag tcgatcactt
     2041 ctaatactag aaacaattaa attatataaa ctgtgttgga tcccatctct tcaaatccaa
     2101 ctgttaggtt cattcatagt attaactcaa gttcagctat taaatcaaaa tctcagtgat
     2161 gcgttttttt atgatggggt gtataaattg tttggcaaaa agaatttaaa atggttaggt
     2221 gtcgtagcaa cgttaatgat gacatttgta caacttggtg gagccttagt taccaaaacc
     2281 ggatcagctg atggttgtgg ttcttcttgg ccactatgtc atggtgcgtt gattccagaa
     2341 ttctttccta ttgatacgat tattgaatta agtcatagag ccgtttcagc tttgtcttta
     2401 ttaatggtct tatggttagt tatcactgca tggaaacata taggctatat taaagaaatt
     2461 aaacctttat caatcattag tgttggattc ttattattgc aagcattaat cggagctgct
     2521 gctgttattt ggcaacaaaa cgattacgtt ttagcattgc actttggtat atcattaatc
     2581 agtttctcat ctgtattttt aataacattg attattttct ctatagatca aaaatatgaa
     2641 gctgacgaat tatatatcaa aaagccatta agacgtttaa catggttaat ggcaatcatc
     2701 atttattgtg gtgtttacac tggtgcacta gtgcgacatg cggatgcaag tttagcatat
     2761 ggtggttggc cattgccatt ccacgatctt gtaccacatt cagaacaaga ttgggtccaa
     2821 ctcacgcatc gtatcatggc ttttatagtg tttacgatta ttatgattac ttatattcac
     2881 gctgttaaaa attatccaaa taaccgtact gttcattatg gttatacagc tgcatttata
     2941 cttgttatat tacaagttat cacaggtgca ttgtctatta tgactaatgt taatttgata
     3001 atcgcactat tccatgcatt atttatcact tatttatttg gtatgacaac atactttatc
     3061 atgctaatgt tacgatcagt aagaagtgac aagcaataac aaaaaagcaa agaagctgtg
     3121 acataaatca atgttcaatg ctctacaaag ttatattggc agtagttgac tgaacgaaaa
     3181 tgcgcttgta acaagctttt ttcaattcta gtcaaccttg ccggggtggg acgacgaaat
     3241 aaattttgcg aaaatatcat ttctgtccca ctcccttttt atatcaatat ttaaaattaa
     3301 acctaagaca agtaaaaacg agattacacg tcaattgttg tgtaatctcg ttttatttta
     3361 atcattttag tcagttgctt tttcaatttc gattaataaa tcgcctgtcg ctattgtgtc
     3421 accattattt acagttactt gtttaatcac accgtcaaat ggtgcttgaa ttgttgtttc
     3481 cattttcata gcttcagtaa ttagcaacgg ctgattagct ttcacagttt cacctacact
     3541 aaccttgact tcagttactg aacctggcat ttgcgcaccg atatgacttg gattactctt
     3601 atctgctttt ggcttaacgt tcgcatttgt atgcacattt tcatctttaa tataaatacg
     3661 tctcgcttga ccattcatcg catagtaaat cgttctatta ccattttcat ctggttcact
     3721 aatcgtttct agtttaataa ttaatcgttt acctttatcg atttcgattt ctactgtttc
     3781 accattacgc attccaaaga agaatgtagg cgtatcaagt aacgataagt ttccgtattg
     3841 atttctagtt tgaatatatt gttcatatac ttttggatat aatacataac taataatatc
     3901 ttgctccgta acaggacctt gttgctcttc ttcaagcaac tcacggactt tttcaaaatc
     3961 aactggctct agatattcac ctggacgagc tgttaatgct tcttggcctt ttaaaataac
     4021 cgcttgtaaa tctttattaa aaccatttac aggttgtcct atttcacctt tgaagaacga
     4081 cactactgat tctgggaaat ctaatttata gccatctgta ataacggatt gttcatcaag
     4141 atcattttgt accatataaa gtgccatatc accaactact ttagacgatg gcgttacttt
     4201 tacgatatca ccaaataaga aattcactct gcgatacata tctttgactt catcaaatct
     4261 ttcgcctaaa cctaaacttt tagcttgttg acttaaattc gaatactgtc caccaggcat
     4321 ttcatgttga taaatttcag tattcggtga tttgatatca ctttcaaagt ctgaataata
     4381 agtacgtaca gttgaccaat aatgactaag tgactccata ccttcaatat cagttctaag
     4441 gtgacgtggg aagccattta atgcataata taacgaattg gcgcttggct gacttgttaa
     4501 accactcatt gaagcaacag cagtatcaat gatatcgaca ccagcatcta ttgcttgttt
     4561 gtacgttaat aaaccattac cacttgtatc atgagtgtga agatggattg gtaaatctac
     4621 agctgctttt aactcaccaa tcaattcgta agcagcttta ggttttaata agcctgccat
     4681 atctttaatc gctaaaatat ggaaaccttc acgttctaac tctttagcta gtttgacata
     4741 atactctaaa gtataaatgt ttgatcgctc aggatttaaa atgtcacctg tataacaaat
     4801 agtaccttct gagattttgc ccgcttcttg tactgcttca ttggcaactt tcatttgatc
     4861 tacccagttt aatgaatcga aaattctaaa gacatctatg cctgctttag cactttcttg
     4921 tacgaattta tgaataacat tatcaggata gtttttataa ccaactgcgt ttgaagcacg
     4981 taacaacatt tggaataata catttggaat agctttacgt agacgttcaa gtcgttccca
     5041 tgggttttcc ttcaagaaat tatatgccac atcaaatgta gcaccgcccc acatttctag
     5101 tgagaaacca tctttaaata cgtccgctgt tttggatgcg atattaatca tatccttagt
     5161 tctaactcgt gtagctaata atgattggtg tgcatctcta aaggttgtat ctgttagtaa
     5221 gacatcatcc tgctttttaa cccattcagc tacacctttt ggacctactt catcaagcaa
     5281 ttgtttcgta ccactaaatg aagcgatttt acttgaagat acagttggaa ttgatgctaa
     5341 ttcatagtct ggtttcggac gtttctcaac atttgggaaa ccattaattg ttacattacc
     5401 tatatattct aatgttttag tacctctatc tagagacggc tgaatgtcga aaagttctgg
     5461 tgtttcttca ataaatttag ttgtgtaatc accacttgtg aactttttat tcttcattac
     5521 attaattaag aatggaatat tagttttaac accacgaata cgcatttctc gtaatgaacg
     5581 taccattttt tcttctgctt gtttaaatga tatcgcatgt gtagataatt ttacgagtaa
     5641 tgaatcataa taaggtgata tctcagcacc ttggaaacca tctccagcat caagacgtac
     5701 accaaagcca ccgcttgaac gataagcaat gattgttcca gtatccggca taaaatcatt
     5761 taacggatct tctgttgtaa tacgacattg gatggcatag cctaatgttg taatatcttt
     5821 ttgttgcggc atattaatct cttcaccaaa taaatcggca cctgctgcaa ctaaaatttg
     5881 tgtcttaaca atatcaattc ctgttaccat ctctgtaatt gtatgctcta cttgtacacg
     5941 agggttaact tctataaaga agaattcgtc accagatact agaaattcaa cagtacctgc
     6001 attgacatat ttaatatttt ccatcaattg aattgcagca tcacaaatac gttgacgtaa
     6061 tgttgatgat aatccaactg atggtgcaac ttctacaact ttttgatgac gacgttgtac
     6121 tgaacaatca cgttcaaata agtgtacgat atttccatgt tcgtcaccta tgacttgtac
     6181 ttcaatatgc tttggattat caatgtatct ttctatgtaa acttcactat taccaaatga
     6241 tttttctgct tctgattttg ctctatggaa agcatcttct aattcacttt cttcacgaac
     6301 gattctcata cctttaccgc cgccaccact tgtggcttta atcattagcg ggaaaccagc
     6361 ttcttctgca aattcttttg ctaattcata tgatttaatt ggaccgtctg taccaggaat
     6421 aactggtaaa tctgccttga tagccgttgt acgagcttta actttatctc caaacatatc
     6481 taaatgttct aaatgaggac caataaattt aatcccttct tctgcacaac gacgcgcaaa
     6541 ttgttcattt tcacttaaaa atccatagcc aggatgaatc gcatccacat tcgcttgttt
     6601 tgctacatca atgatacgct caatatttaa ataactttca gcaggaccta aatcacttcc
     6661 aactaaatag gattcatctg ctttatatct atgtaatgaa cttttgtctt cattcgaata
     6721 aattgcaact gtgctgatgt ctaattctgc tgccgctctg aatatacgaa ttgcaatttc
     6781 tccacggtta gcaacaagta acttttttat ttgtttcaat agcgatacac tcctcaaact
     6841 attagaattt tctaactaat tagataataa aattttatct taaagcgctc tgttttgcta
     6901 tagttatgtt tcaaattttc aaaatttaac attcttgaga caattaaaac ctccgcttca
     6961 aaaataataa tttcaaaaag gattatgcaa caacaggtag ttcacgtttt tgttgtgata
     7021 cattttcgat ttctacaact ctaaaaaatt aaaaataaaa ttgtaaaaca tcaacattta
     7081 ttatcaatag cgataacttt atcttatcat catgattcta atttcgccac cacatttagt
     7141 gatttttagt cataaaattt agttataatt atacgttgtt ttgtttataa aatttgataa
     7201 tcatgagtaa tctcgtaata tcaaaacaaa aaggaagtta agcgttgttt ggttgcttaa
     7261 cttccgttat tgaactcatc agtccaattt gacaaagagt atataaatag ccaatgtgta
     7321 gcattgctta atgacagaag tcatctcgcg ctaaatattg gctagtattt tttaattaaa
     7381 ttgtcttctt atatcaactt tttgttgttg tttctttcgt tgctggtcta ctttgatttg
     7441 tttacctaca atcagaagta atcccatagc aatacttaaa ctaatcattg atgatccacc
     7501 aaagctgata aatggcaatg gcacaccagt taatggaatt gttgccgaaa taccaccaat
     7561 gtttacaaac gtttggcttc caaagtatgt ggcaatccca acacacacaa gtttataaaa
     7621 atatgatgat gttttatttg caaactggaa ggcacgatat acaataaaga actctaaagt
     7681 aataactagc aatcctccga ttaaacctaa ttcttcgcaa ataattgcaa aaataaaatc
     7741 tgtatgtggt tctggtaaat agcccaattt cattgcacta tttcctaatc cttttccaaa
     7801 tacgccaccg ttacctatcg caagcaatga attggaaata tggtatccag ttcctgattc
     7861 gaattggaat ggatctgtta gcgtactaaa tctggcagtt aaataacttg gtaaccaacc
     7921 agccattaat gcaatgacaa atactactaa gaatcctagt actgctggta ttccaaatct
     7981 taggactttg tttaccccaa ttcctgaata aaaaataatc gcaactaaaa taattaatat
     8041 tagtaatgtt tgccctacgt ctttttgtaa gaaaactaaa aacgtacaac ctaatgctaa
     8101 tacaatagga cttaaaatta attttggttt acttaatact ctaggcattt ttttactaat
     8161 cataaatggg atatataata tgattgcaat ttttaataac tcagatgcct gtaagttcat
     8221 aaatcctaag tttatccaac ttttagaacc attaatatct ttaccgatta ctaacgtcag
     8281 taataatagc gagacgatag ttataatcat acctttttgc actttaatat tactcagtaa
     8341 tttaacattc attaaaaatg caataaaaaa tacaattata aaactcatta tgacatatgc
     8401 taattgtcgg ttgtaaaaat acgttcctgg aacatcgata ccaccagtca atgtaccttt
     8461 agttgctgga accatactgg cactatatac catcactaat ccaattaaac tcaatacaat
     8521 atatgtaact aataacgggt aatcaataaa ctttgaggtt ttaccaatat accgtaaaat
     8581 acttctaaaa ttcttcatat attcagtcat ccaattctac aatttatact tcaaatgatt
     8641 tcgttttata ctttaatttt aaaaatccat gttacttatt atatacatga cacagtcata
     8701 atcacaaata ttaacaggtc tcttgaggta atttatagaa tatttgttta cttaatatcg
     8761 tgaattgcta aaacccaata aaagtgactg acatgtcatc aagaagacaa cataacagcc
     8821 actttaatca ataattaaat tataaatata aaatcggcgc tgctcaattg tcgatatctc
     8881 acgccgtttt aatatgctta tatattaaac cttaaacaag tgtaaaagct tcatgtaatt
     8941 ttgaaagttc tttctcaaga cgtaacataa tttgtttgcc atcttcgcgg tcaactaaac
     9001 ctaatttaac agcaaaatca acttcttttt gaagtccaaa catttgtgta tctagtactt
     9061 cttcatataa tgggcatgaa ggtaatgtta aattatctag ttgaacttta atcagatgca
     9121 agatttcatc agcatcttta gtcaattgtt tcaaagctgc atttttcatt gttgtttgtt
     9181 tcgccatgac acgctccccc tctatataaa ttctcaagtc ttactaaaag tttatcttac
     9241 taaatattaa aaagcaagta gatttaaaat gtgtcgttag caatttgtgt taaaataatg
     9301 ctatatacat tgatagggag tgggaaaatg atacaaatta aaggagctgt aaagtttcct
     9361 ataacattag atagtacgac ttggattttc gatgatagaa aagttagcat cgaagattta
     9421 gaaaaaggtg tatttgatgg ttcaaaacca atcgactttg aagataatca tgaatggaat
     9481 cgtgctatcc tagaaggaca aactaatccg cctactttaa attcagaaat aaaatataaa
     9541 aaacgtgcca tgttagaagg ttcatttgtc attaatatga caccattttt caaacattcc
     9601 gaaccacatg actctgcaac tactattcga ttatcaaatg agcatgatca tttagatata
     9661 ccgatagatc tattgcccta tttatttttc caatttgcaa aagacggtaa acgactttac
     9721 agcgataatg gtgtagatag ttttatttac aatcaagaag atggttattc atatcgaatc
     9781 aaccatgtaa ctcatattga ggtgatttaa tttgagacaa gtgcaatgca ttatctgtga
     9841 tgcaaaagta tttatagatg agcgcacaac tgagtctaaa cgacttaaaa acaatcctat
     9901 tcgaacattt atgtgcgatg attgtaaaag tcgactagac acccctaaac aacgtgcgca
     9961 acactatccg ctcgattaat atctgaatta acaagttaga aatttgaaaa tgtgaaagta
    10021 acaggaactg atagcattaa aacacttttt caaatattat atgccaaaag agaagctaag
    10081 caactttcat cgctcagctt ctcttttatt atgttaatta ttcattttct tgcatcattt
    10141 gtttgatacg ctttgcttct ttttcacgaa catttttgtt taaaattttc tttcttaaac
    10201 gtatgctttc tggagtaacc tcaacaagtt catcatcatt aatgaattgt aacgcttctt
    10261 ccaatgttag tatacgcggt ctattcattg tttgtgtttg gtctttcgtt gcagaacgta
    10321 cgttagtttg atgttttgtt ttagtgatat taacagttaa atcattttca cgattatgtt
    10381 caccaacaat cataccttca taaacttcag taccaggttc catgaagttt acacctctat
    10441 cttcaagtcc taaaatggca taagtacttg cagaaccttg atccattgaa attaacgcac
    10501 cattacgacg accgccaatt tgcgctttaa tacgtggtct aaattcttca aatgtatggt
    10561 taataatacc gtaacctctt gtcattgaca taaattcagt cgtataacca atcataccac
    10621 gagccggtac attaaagatt aaacgtgtaa gtccattatc agttgtagtc atatcaacca
    10681 tttcaccttt acgtgcacct aatgattcaa taaccgcacc tgcattttct tgtggcactt
    10741 cacattgcac acgttcaaat ggttcacaca ttacaccatc aatttcttta ataattactt
    10801 gtggttttga aacttgtaat tcataacctt cacgacgcat attttcaata aggattgata
    10861 aatgcaattc acctcgacca gcaactaccc atgtatctgg agaatctgtg ttagaaactt
    10921 tcaaagatac atctgtttct aattgttgat ttaaacgttc ttgaatttga cgcgctgtta
    10981 caaagtcacc ttcacggcca gcaaatggtg aattgttaac tttaaatgtc atttcaagag
    11041 taggctcatc aatacgtaga actggcaatg cttcttgatg gtcatgtggt gttacagttt
    11101 caccaacatt aatgtcttcc atacctgaaa cagcaattaa atctccagct tgtgcttctt
    11161 caatttctaa acgttttaat ccaaagtaac caaagatttt agttacacgg aagtttttca
    11221 ctgtaccgtc taatttaatt agtgatacat tatctccgac acgcatttta cctctgaata
    11281 cacgaccaat accaatacga ccaacataat cattgtagtc caacaatgct acttggaatt
    11341 gtaatggctc atcactgtta tcaattggag ctggtacata atcaataatt gtttcatata
    11401 atgattgtaa attatcatct tgtttttcag gatctaagct agctgtacca tttactgctg
    11461 aagcataaac aacagggaat tctaattgtt catcgtttgc ttctaattca ataaataaat
    11521 ctaaaacttc atctacaaca ccctctggac gtgctgatgg tttatcaatt ttattaacaa
    11581 caacaacagg ttttaggttt tgttctagcg cttttttaag tacaaaacgt gtttgaggca
    11641 ttgtaccttc atacgcatct actactaaga caaccccatc aaccattttc ataatacgtt
    11701 ctacttctcc accaaagtca gcatgtcctg gtgtatccaa aatattaata agtgtacctt
    11761 tataatcaac agccgtattt ttggctagaa tcgtaattcc acgctctctt tcgatatcgt
    11821 tagagtccat tgcacgttca tcgacatgtt cattttctct gaatatacca gattgtttta
    11881 acaactcatc tactaaagtt gttttaccat ggtcaacgtg agcaataatt gctatattgc
    11941 ggacatcttc tcttttatta gtcatataaa tagtttcctt ctttcttaag taaaatccca
    12001 cttttaatta caactttttt attatatcat ataattgaga aaagataagc aaaaggtggg
    12061 gataggatga aacaaaaaaa atctaaaaat atcttttggg tattttctat attagctgtt
    12121 attttcttag tattatttag ttttgctgtt ggtgcatcaa atgtaccaat gatgatttta
    12181 acatttatat tactcgttgc aacctttgga attggattta ctacaaagaa aaaatatcga
    12241 gaaaacgatt ggctataagt tatatcgaca ataacacgcg ttgattaaag accacacatg
    12301 ttcatgatga acatttaaat ggttttatat tggcgcgtgt ttttattatt taagatataa
    12361 aaacgtaata tgtcacctaa ttgttaggac tacatattac gcttttggta attattttga
    12421 ttttctttta aatcgttgtt catgtaattg tattaacgca tcgcgatgtg gctctaaata
    12481 tacaccgagg atctctctat gaacaccacg attaccaact aagattgaat ttggaccact
    12541 aattgttaat ggttctccta gtaaattaga agctagaccg tccacctcat ttaaaatgac
    12601 tagtcctcca gcaaaatccc acggttggag tctaggtgtc atatatgctt ctaaattacc
    12661 ggtagcgaca gaaacaattt caagcgctgc actaccatat gcccttgcac ttcttgaaat
    12721 atttacgaca tccttaaaaa tctctcctag cacaggttta gtcaaccagt tcggattgat
    12781 cccaataatg ctttgtctta gattagaatc attcaatggt ttcaagggtt ggctacctcg
    12841 atatgcacct tcccctactt tagcatgata taagacatca gccataacat catatacaaa
    12901 acctgcataa ggtttaccat cgatataaat accaattgaa atcgcgaaat tttcttgttg
    12961 atgaacaaaa ttcaatgtac cgtctattgg gtcaacaacc cataccgtac ctttggaagt
    13021 atcgatgtca tgaccatgcc cttcttcacc taatacttga tgattgggat atgtttctaa
    13081 aattgtatca aaaataaaat cttctgttgc tttatctaca tttgtaacaa ggtcattcgg
    13141 atttgacttt gtttcaattg ttaaattttg ctccatcaat tgtttaattc taattcctgc
    13201 ttcttgaata agtccttggg caaatccata aagtgccata acatcatctc ctaacatctt
    13261 tattatacat caacatttta taaaaataac ttcttatgat aaaaatgaaa atactaattt
    13321 taatgagagc gtcttaatcc gcaattgata aaacatatgc tacaatattt ttaaacacta
    13381 tacaggaggt actctatgac aaaatataca tttaaaccta aagatttcaa agcattcaac
    13441 gtagaaggct tagacgcacg aatggaagct ttaaacgaat acatacgacc acaactccat
    13501 gaattaggag aatattttag tgacttcttt acaagtcaaa ctggtgaaac attttatcct
    13561 cacgtagcaa agcatgctag aagaagtgtg aatcctccta aagatacatg ggttgctttt
    13621 gctacaagta aacgaggcta taaaatgtta cctcatttcc aaattggtat gtttgaagat
    13681 caactgtttg ttatgtttgg aatcatgcat gaagcaaaag ataaagcaac acgtgcaaaa
    13741 gtttttgaaa gaaaatttaa agctattcaa caattacctg atgattatcg tgtatgctta
    13801 gatcatatga aacctgataa accatttatt aaagatttaa cggatgatga tttaaaagaa
    13861 gcgatacaaa gagccatcaa tgtgaaaaaa ggtgaattct ttatagcgcg tgcaatcaca
    13921 ccacaagata aaagattaaa aagtgacaaa gcatttattg catttttaga agaaaccttc
    13981 gatcagttct taccatttta ttctgcataa ataactttgt ttaaataata gagcacgtaa
    14041 tcacatccat gatttcgtgc tctttttact taccattaaa ttgaacgttc aacataataa
    14101 ttcatacttt taaaaaaatt aaaataaatt taggttgacc taaacgtttt attaggttat
    14161 tatattgtcc ataagaagta gaggtgagtc aaaatgaata ataaacgaca ttcaacaaat
    14221 gaacaattaa gtttagacga aataaacaat acaattaaat tcgatcatcg cagttcaaat
    14281 aaacagaaat ttttatcatt tcttgggcct gggttattag tcgctgttgg ttacatggat
    14341 cccggaaact ggataacatc aatgcaaggt ggcgcgcaat atggctatac tttgctattc
    14401 gtaattctta tttcaagttt atcagcaatg ttacttcaaa gcatgacagt gagattagga
    14461 atagcaacag gtatggactt agctcaaatg acacgacatt atttatcaag acctattgct
    14521 ataatctttt ggattattgc agaactagca attatcgcca cagatattgc tgaggttatt
    14581 ggtagtgcta ttgctctcaa tctcctattt aacatacctt taattgtcgg tgcactaatt
    14641 actgtacttg atgtattttt actactattt ataatgaaat atggttttag aaaaattgaa
    14701 gctattgttg gtacattaat tttcacagtg ttattcatct ttatatttga agtctatatt
    14761 tcatcaccac agttgaacgc tgtgttaaat ggatttattc cacatagtga aatcattaca
    14821 aataacggca ttctctatat tgcattaggt attattggag caacaattat gcctcataat
    14881 ttgtacttac attcatcaat tgtacaatct agaacatact caagacataa caatgaagaa
    14941 aaggcgcaag cgattaaatt tgctacgata gattcgaaca ttcagttatc aatcgcattt
    15001 gtagtcaatt gcttattatt agtgttagga gcatcactat ttttcaactc aaacgctgac
    15061 gatttaggtg gtttctatga tttatatcac gccttaaaaa ctgaacctgt actaggtgca
    15121 acaatgggtg caatcatgag tacattattt gcagttgcat tattagcgtc aggtcaaaat
    15181 tcaacgatta ctggtacttt agcaggacaa attgtaatgg aaggattttt aagattacac
    15241 ataccaaatt ggttaagacg tttaattaca cgttcccttg ctgtcattcc tgttatcgta
    15301 tgtttaatca tttttaaagg taatgcagct aaaattgagc aactacttgt attctctcaa
    15361 gtatttttaa gtattgctct gccattctgt ttaataccat tacaattagc tactagtaat
    15421 aaagacttga tgggaccttt ctataataaa acatgggtta atatcatttc atggacactt
    15481 attattattc taagtatttt aaatgtctat ttaatcgttc aaacattcca agaactacaa
    15541 ggttaaaaat aaaaccatta aaatgttggt tgtatctaat aattaacaac ttaacatttt
    15601 aatggttttt cttttgctat gaacatatta atgataaata tactcatcgt ccttatgggt
    15661 agtttctttt gattcttttt tagaaatcaa cataatcatc acgataatcc ataagacaat
    15721 tgcaattaaa ttaagagcca tcaaacttac aattatcgcg ataacaaaaa gtataatcga
    15781 taattttcta cgtcctttaa tacaaattgt cgcaaatata ccataaagtg ttgaaattaa
    15841 taaaataatt gctaatgcac ccattgttgt ctgaagcata tctaaagaaa ccttgccacc
    15901 gttctgagat aattgcttcg ctaattcttc acgttgtgat gcattaccac tttttaaaga
    15961 aaaataggat agccccataa ctaataaata aataatacta atactatttg caatccaagc
    16021 catgattaaa tcaggttttc
//
LOCUS       DATTQF010000051        15647 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.051, whole genome shotgun sequence.
ACCESSION   DATTQF010000051 DATTQF010000000
VERSION     DATTQF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.051"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            67..831
                     /locus_tag="VB241_001357"
     CDS             67..831
                     /locus_tag="VB241_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1244656.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHVDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(889..1395)
                     /locus_tag="VB241_001358"
     CDS             complement(889..1395)
                     /locus_tag="VB241_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEP1244657.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGNYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDSMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(1565..2875)
                     /locus_tag="VB241_001359"
     CDS             complement(1565..2875)
                     /locus_tag="VB241_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEP1244658.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFSWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLVAILFNFPKLKKNTSQEMK"
     regulatory      complement(2952..3047)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3100..3669)
                     /locus_tag="VB241_001360"
     CDS             complement(3100..3669)
                     /locus_tag="VB241_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244659.1"
                     /translation="MALIVSRAILFLILALALGIATVFPEQKLPVYILFTLFVVVAIV
                     SNVWLTHQNVKKHIIKFDKHVAYFLGVLSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIRNTRPSNKQLKVISEQSAQQFKVISLILVSVSIVFIFIALMLKW
                     WLILAISIIVLIIITVAMIVYEKYYTTPN"
     gene            complement(4156..5037)
                     /locus_tag="VB241_001361"
     CDS             complement(4156..5037)
                     /locus_tag="VB241_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1244660.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGNQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(5148..6269)
                     /locus_tag="VB241_001362"
                     /pseudo
     CDS             complement(5148..6269)
                     /locus_tag="VB241_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
     gene            complement(6544..7782)
                     /locus_tag="VB241_001363"
     CDS             complement(6544..7782)
                     /locus_tag="VB241_001363"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEP1244661.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKHYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTLYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNIQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKV"
     gene            complement(7782..9443)
                     /gene="hutU"
                     /locus_tag="VB241_001364"
     CDS             complement(7782..9443)
                     /gene="hutU"
                     /locus_tag="VB241_001364"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEP1244662.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDTEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIATKYCDIKTANLDEALKLA
                     EEAKERGEGLSIGLVGNAVDLHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFHKRGAVAFDYGNNIRQVAFNNGVRNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            9603..10487
                     /locus_tag="VB241_001365"
     CDS             9603..10487
                     /locus_tag="VB241_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1244663.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLCRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            10729..11148
                     /gene="fosB"
                     /locus_tag="VB241_001366"
     CDS             10729..11148
                     /gene="fosB"
                     /locus_tag="VB241_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HEP1244664.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(11404..12339)
                     /locus_tag="VB241_001367"
     CDS             complement(11404..12339)
                     /locus_tag="VB241_001367"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEP1244665.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTGYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFIHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            12617..13876
                     /gene="sdpC"
                     /locus_tag="VB241_001368"
     CDS             12617..13876
                     /gene="sdpC"
                     /locus_tag="VB241_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HEP1244666.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYDTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTES
                     QHHIDNQTESNHDEDHDITSKSVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(13942..14628)
                     /locus_tag="VB241_001369"
     CDS             complement(13942..14628)
                     /locus_tag="VB241_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEP1244667.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            14799..15452
                     /locus_tag="VB241_001370"
     CDS             14799..15452
                     /locus_tag="VB241_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEP1244668.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
BASE COUNT         5102 a         3133 c         2187 g         5225 t
ORIGIN      
        1 ttctaaaatt tcacctttta atgtaaaaac catatatcac aaatttattt tccaaggagg
       61 ttgaatatgt ttttagacga acacattaat agaaactttg acaaactaaa tgataatgat
      121 ttacatatcg cccacttcat caacacacac gttgatgaat gtaaaaatat gaagatacaa
      181 gatttagcgc aattcacaca tgcctcgaat gcaaccattc atcgattcac acgtaaatta
      241 ggttttgatg gttatagtga ttttaaatcg taccttaaat ttgaaagtaa taagacacac
      301 caattaccat ctaactctat cgatagtttc aagcaagaaa tcgatagtac attcagttat
      361 ttagaacgcg ttgattacca gcttcttact gaaaaaatgc atacagcttc aacaatatat
      421 ttgtatggta cagggcgtgc acagttaaac gttgcttcag aggcacagcg tatattatta
      481 acgttacata atcatgtcat tgtacttcat gatgaacatg aacttaaaat gattttgaac
      541 aagagtgaac ctaatgattt attctttatt atttcattat caggtgaaac acatgaatta
      601 ttagaagtaa caaacttgct tcaacttaga caaaaatact ttatttctgt tactacaatg
      661 aaagataata cccttgctca aaaagcaaat tacaacgtct atgtatccag taacacattc
      721 tacttaactg atggtacaga ctattctagc ttcatcagtt atcatatatt ctttgaaaca
      781 cttgtaagaa aatataatga gtatttacta cataatgata tttcaaatta gaaaaaagga
      841 agttatgatt taatggcctt gagcctatca taacttcctt ttatcatttt attgttgtgt
      901 tgatgtttcg ataacgtggt acattttatc aaacatcaat tcgaaaccat gcaccatgga
      961 atcatgatat tcttttttct tttgcttgta ttctaaatta gtaaatcgtc tttccttttc
     1021 aactaatgaa cgataataaa atagcatttg ggtgccacct gtttcacgtt caaaaaattc
     1081 tacctcaatg acatcttgtg tttcacttag tccaggcata ccgatagtca tcttaacgta
     1141 ttcatccata actaaagatt cataaatacc ttcaatcaca tttactttgc cattacgttg
     1201 ttgatctaca atacgataat taccgccttc tttaacatcc gcttcaatct ctttattcgt
     1261 tctggctgat gtcataaacc attgttttaa caaatctttc tttgtccaag cttcgtatac
     1321 taactctgga gaaaatttat aaagcttttc aatttcaact tcgacatgtt cattctctac
     1381 attaaatttt gccactgttg tccacccact ttcgctctta cttttatttt aacgtatttt
     1441 tgctcagttc caaacataga tgatcatcat ttttaaaaga ttagcgttat acggtgagta
     1501 caacatgatc tgttaatata acaagccacc ttacttggct acatcgatat attgttaagc
     1561 attactattt catttcttga ctagtgttct tttttagctt tggaaaatta aataaaatcg
     1621 caacaagtcc gcatacacct aataatatag gataaatgct atatgggaat aacattaacg
     1681 gtgaaatacc agctacacca gccgctgaaa tgacttgcgg gctatatggt aataaacctt
     1741 ggaagcagcc tccaaatata tcaagaatac ttgctgattt ccttgaatct acatcatatt
     1801 catctgcaat atttttagct aaaggacctg acataataat agagatggtg ttgtttgccg
     1861 tggcaatatc tgcgacactt accaaactcg caattcctaa ttctgcgcca cgctttgatt
     1921 tcactttaga gcgaacaaat tgcaacaacc attcaatacc accattgtgt tgaataatac
     1981 cgactaaacc accaattagc aatgcaatca tagcaatatc ttccatgctt ataatacctt
     2041 tggacactgc atctagtagc cccatccaac tgaatgaacc atctatgaga ccaatgattc
     2101 cggctaataa tgttccgcca atcaatacga taatgacatt tacacctaat aatgctaata
     2161 ccaatactaa aatatacggt acaactttaa ttaaattata atcatagttt ttagcatgat
     2221 ttaaagaaat gccattcgtt aagaaataaa gaataataat cgttaaaata gcacctggca
     2281 atacaatttt aaagtttact ctgaatttat ctttcatttt cgtatgttgt gttctaaccg
     2341 cagcaattgt tgtatctgaa atcattgata gattatcgcc gaacattgca cctccaacaa
     2401 ctgtagccat tgctagcgca gttggtacat ctgtcgcttg tgcaaatccg taaccaacag
     2461 gtgctattgc agcaacagtc cctactgacg ttcccataga tatagaaaca aacatacaaa
     2521 tcacaaacaa tcctacaata attaaatttt ctgggattaa tgatagtcct aaattaactg
     2581 tcgactttac gccacccatt ttttcagctg tatttgaaaa tgcacctgct aaaataaaaa
     2641 tcaacatcat taaaataatg tttgaatggc ctgcaccttt cgtgaaaacc tcaacttttt
     2701 tagcaaatga ttcttttcga ttcattaata acgccacaat taccgttatc gtaattgcaa
     2761 catttaatgg cattgaagta aaatcacccg tgataatacc tacgcctaaa aacaacgcca
     2821 caaataataa caaggggaat aatgcccaag cattgctctt tttatgtact tccatccttt
     2881 ttacctgctt tccaattaaa aatacctctt tctcacaaac gatgaagaaa gaggttttca
     2941 tgtactttac ctgcttatct tcaaaccatt acggttactg gaattggcac attcgagatg
     3001 ttgccgaggc ttcatagggc cagtccctcc acctctctag ataagtgatg cttatttacg
     3061 tttacgttac aagataatcc ttagtacgtc aatcataaat taattaggag tcgtataata
     3121 tttttcataa acaatcattg ctactgtaat aataatcaaa acaataatgc taatcgcaag
     3181 tataagccac catttaagca ttaatgcaat aaaaatgaac acgatagaca cacttactaa
     3241 tattaatgat atgactttaa attgctgtgc actttgttca gaaataactt tcaactgttt
     3301 gtttgatgga cgcgtatttc ttatactgat tcccagtata ttttctaata tttgaaccaa
     3361 tacgatactt attgcaaata taataattgg taaaacatca tagctcccta tagttaatgt
     3421 ataaattaca aatccaatgt aaagtaaccc tgagagcaca cccaaaaagt aagcaacatg
     3481 tttgtcaaac ttaatgatat gttttttaac gttttgatgt gtaagccata cattcgaaac
     3541 aatcgcaacc acaacaaaca gtgtgaataa tatataaact ggcaactttt gttcaggaaa
     3601 aacagtcgct attcctaaag ctaatgctaa aatcaaaaat aaaatagctc tagatactat
     3661 taatgccata ataacaaccc ctttgtttaa tatcgagttt gcaaatttac gtttatcagc
     3721 atttctatga tcagtacttc tacggtcagc gtttctatgt aatttacatc atcttaacat
     3781 ataaatactt cgctatttaa ttgaaaacat atcctattat tctttgtccg ttctgacgtc
     3841 taatatctaa ccttaggcat tacacttggt aataaattta actttcttcc actaaccgcc
     3901 actaaaccca atcccgcaat agtttttaac tttttcgttg ttgtcctgac atcctcatta
     3961 agaaagttta ttctgcttaa aacttataat ccacaccctg agcaaacgct ccttatgaca
     4021 aagtattaaa ataagccgat aaagatacac acctttaccg actatttaaa atacacttca
     4081 ccaattcatt ttaatttact tgattgaagt agctaaatta atattatgtt gttcaattaa
     4141 aagcttcata caaacctaat ctatttgcac tccaccgcta acaccgaaca cttgtccagt
     4201 tgtataactt gattcttctg acgctaatag cacataagtt ccacataact caacaggttg
     4261 acctgcacga cctaaaggtg ttttttgacc aaatgttggg attttacttt gaggttgtcc
     4321 accagaaatt tgtaatggtg accagaatgg accaggcgct acacagttca ctctaattcc
     4381 ttttggtcct aattcttctg aaaaactttt agttaatgaa ataattgctg cttttgaagc
     4441 ggcataatca tgaagaatag gactaggatt ataaccttgt acagacgatg tcgtcgtaat
     4501 tgacgcaccc ggttttaaat attccaaagc tttttgaact gtccaaaata gcggatagac
     4561 attcgtttca aatgtttcag taaatgcctc agttgtaaat ccatgaatat catcatgata
     4621 ctgttgatgt ccagcaacta aagtaacatt atctaagcca cctaattgtt gatatgcttg
     4681 ttcaacaagg tcatagttga actgctcatc tcttatatca ccaggaatta acactgcctt
     4741 ttgaccactt tcttcaatca cttggcgtac ttcttgtgca tcttgttctt cactcggaag
     4801 atagttaatc gctacatctg caccttcttt agcatacgca attgctgctg cacgccctat
     4861 tgctgagtca ccacctgtaa ctaacatttt atagccttgt aagcgttgat taccttggta
     4921 agacgtttcg ccacaatcag gtgctggcgt catttcagat tgtaaacccg gtacctcttg
     4981 ctcttgtttt tcataatccg tggttttaaa ttttgttcta ggatcttgag ctgccatttt
     5041 taaacatctc cttattcgct taatggttat tatttaccca atcttcctag gaacttaatc
     5101 atgattacac taaaaattac tttcttcttt ataaaaacaa gctcgaatta ctcatgcaat
     5161 agtctcttta caaattcaac aaaatactca ggtacttttt ctagaatcct ttcatccggt
     5221 ttatattgag ggtgatgtaa atcatattca ctatgagaac caattaacgc aaatacactt
     5281 ggaaaatgtt gactataacc tgaaaaatct tctccaatcg taagcggctg ttccatcatt
     5341 tccaccttat atccaacatg ttaggctact gctattgctt tatgcgtcaa tgcctcatca
     5401 ttcatcacag cgccaggtaa atgtgtataa tttaaattaa ttttcatatt atatgcttga
     5461 gctaacccgt ccgcaatatc ttgtaatcgt gtttctacaa gctttcgcac cacaggatca
     5521 aaactacgca ctgtgccttg tacatacgca tgatcagcaa tgacattcca agtattacca
     5581 catgatattt gtccaattgt tactaccgct tcatcaaacg cagatagatt tctactaact
     5641 atggattgaa tactattaat caattgcgcc aacacaataa ctggatcgtt gcattgttct
     5701 ggctttgcag catgaccacc cacgccttta atatgaaact caaaacgatc tactgctgat
     5761 gtaattgccc ctgttttgat tgcaaatgta cctaccggac gcgatgggtc attatgaaaa
     5821 cccaatactg cttgtacatc ttgtaatgca tgtgtttcaa taattttaaa agcgccatgt
     5881 cctagttctt ctgctggttg aaaaatgaat ttaacacgcc cagtaagagt gccctcaatt
     5941 tcttttaact ttacagctgt agccaaaata ctagccatgt gaatatcatg accacacgca
     6001 tgcatcacac cttcattttc agatttaaaa tcctgttcaa caagctcttg aattggtaaa
     6061 gcatcaatgt cagcccgaac agctatacaa ctcagacctt gccccacttc agcaacaagt
     6121 ccagtcgcaa gtggtaagtc taatattcta atattatatt ctgttaaaat atctttaatt
     6181 ttttgtgtag tcttaaattc tttatcggat agttctggaa attgatgaaa ataccttctc
     6241 caggcaacag cttgatcttt taatcccatc ggtcattccc cttccttaag tcaatgatat
     6301 gttgtctacc ctacgatgat cgtctttaac tattaaacga tgatttcaca acaatgtact
     6361 cttgttaatt actttcgcta atgatagaca gttgtttaat aatatcgtaa cattattgtc
     6421 aaactattct aacttttata attaagaacc tatacaaaaa cgtgttctca gataaacttg
     6481 ttttacaaac cacaaaaagc tctaaacatt agtgtaaacc aatgcttaga gcttttaatt
     6541 actttatact ttaaaagata atgtgttatc tacgatgacc ttaccgtctt taataacttt
     6601 ttctgcgtga ttgataccaa aatgataagg aatatattca tgatttggtg catcccaaat
     6661 tactaaatta gccttatcac ctgtgttaat tgtacccgcg ttaatgtcta ttgctttagc
     6721 agcattgacc gtaacagcat tccaaacttc attaggcgat agctttaatt tcaacgctgc
     6781 aatcgccata acaagctgta tgttgtttgt gacactactt cccggattat aatcagttgc
     6841 taatgcaatc gcaccgttat tgtcaagcat gcctcttgca tctgcataat cttctttacc
     6901 taaatagagc gtcgttgcag gtaagaggac agctacagta tcactatttc gcaacttttc
     6961 ttttcctttg tcactagacg ctactaagtg gtcagctgat attgcttgtt cgtcaattgc
     7021 taattccagt ccgcctaatg gatcaatttc atccgcatgt attttcactt taaaacctgc
     7081 ttctttggct ttttgcatat aatgttgcga ttgctctatt gtaaatacac ctgtttcaca
     7141 gaatatatcc gcaaagtctg cataatgttt tacttccgga agtaacgcaa tcatttcttc
     7201 taaaaatgcc tcatttgaac ttgcctcttt aggtacagca tgaggcccta ggaaagtatg
     7261 tttcatatct aaatcatatt tctcagctaa acgattagac actttcaatt gcttcagttc
     7321 attttctcta tctaatccat aaccactctt actttcaact gcaagcacgc cgtgtttaat
     7381 catagtaagc aaatcatgct ctgctttttt aaacaagtca tcttcggatg tttctctagt
     7441 agcattaacg gtagataata tgccaccacc catttctaat atttcaaggt aagacttacc
     7501 ttgacgtttt aatgacatct catgttctcg agatccacca aatgttaaat gggtatgagc
     7561 atctactaat gctggggaca ctaccttccc actagcatca attgtctcag tcgcatcgta
     7621 gtcatctgta tgtgttccag catatacaat tttgccatct ttaatgacaa ctgtaccatt
     7681 tttcacaaca tttaattcat ctaattcctt acccttcaaa ggtttatctg ttgatctcgg
     7741 taaaattaat tctgctatat gattaattat taaatcattc attacttatc acctgcttta
     7801 tcaatcattg gaatatgaat acctttttct ttagctgttt gaatagcaat gtcatagcca
     7861 gcatcaacat gtcgtgcaac acccatacct ggatcagtcg tcaatacgcg ttccaatctt
     7921 ctttcagcac gctctgatcc atctgctacg acaaccatac ccgcatgaag tgaatatccc
     7981 atgccaacac cgccaccatg atggaatgaa atccatgaac cacctgcagc cgtgttaatg
     8041 agtgcattca atacagccca atcaccaacc gcgtcactac catctttcat actttctgtt
     8101 tcacggttag gactcgcaac tgaaccagca tctaaatggt ctcgtccaat aacaattggt
     8161 gctgaaattt caccgtcacg tacaagacga tttaaagcta agcccatttt tgctctttct
     8221 ccatagccta accaagcaat acgtgatggt agtccttgat atgaaatttt ttcttcagct
     8281 aaatcaagcc atcttaataa cttttcattt tctgggaaaa gtttacgcat ttcttcatcc
     8341 gcacgctcga tatcttttgg atcaccactc aatgcagcaa agcggaatgg ccccttacct
     8401 tcacagaata atggtctaat gtaagctggt acaaagcctg ggaagtcaaa agcatttctc
     8461 actccgttat tgaaggctac ttgacgaata ttgttaccat aatcaaatgc tacagctcca
     8521 cgtttatgga attcaagcat taattcaaca tgctttgcca ttgaagcttg tgacagttca
     8581 acatattttt tcggatcttt ttcacgcaat actttcgctt cttctacgga gtatccttgt
     8641 ggcacatatc catttagcgg atcatgtgca cttgtttggt cagtaataat gtcaatttta
     8701 aatccttttt ctagaatcgc ttgatggagg tctacagcat ttccaactaa cccgattgac
     8761 aatccttctc cacgttcttt cgcttcttct gctaatttta atgcttcatc taaattagct
     8821 gttttaatat cacaatattt cgtagcaatt cgcttatcaa cacgtgtttc atcaacatcc
     8881 acacaaattg ctaccccatg attcatagta attgctaacg gttgcgcacc acccatacca
     8941 cctaaacctg ctgtcagtgt aacagtgcct gctaaatctc cattaaagtg ttgattacct
     9001 agctcagcaa atgtctcata agtaccttgc acaatacctt gagaaccaat atatatccaa
     9061 ctaccggctg tcatctgtcc atacatgatt aaaccttttt tatctaattc attaaaatga
     9121 tcccagtttg cccattcagg cactaatact gaatttgaaa ttaatacacg tggcgcttct
     9181 tcatgtgttt taaatacagc aactggcttt cctgattgta ctaacattgt ctcatctgat
     9241 tctaattcgc gtaacgtttt ctctattgct tcaaaagctt cccaattacg agctgctttt
     9301 ccaataccac cataaacaac taaatcttct ggtctttcag caacttctgt gtctaaattg
     9361 ttgtataaca ttctaagtac tgcttcttgt tcccaacctt tacactcaat actcaaacct
     9421 ttttttgctt gaatttttct cataaaattc gctcctgttc tttttagaag ttaattccac
     9481 taaatttaaa acgcttacat tattatcttc aatattcatt atagtatgtt aaaatatagc
     9541 caacaaatat aaataaacta attatccata gcttgaatct ataaataaaa ggagcaaaaa
     9601 acatgaaaat tattcagtta gaatacttct tggctatcgt gaaatataat agttttacta
     9661 aagctgcaca atttttacat attagccagc catctttaac tgctacgatt aaaaaaatgg
     9721 aagcagattt aggttatgac ttatttacac gttcaacaaa agacatcaag attaccgaaa
     9781 aaggaataca gttttatcgt tatgcgagcg aattagttca acaatatcga tccacgatgg
     9841 aaaaaatgta tgatttaagc gttacatcag aaccaaggat aaaaattggg actcttgaat
     9901 ctacgaatca atggattgcg aatttaattc gaaaacacca ttccgactac cctgaacagc
     9961 aatatcgttt atatgaaata catgacaaac atcaatctat agagcaatta ctgaatttta
    10021 atattcattt agctataaca aatgaaaaaa taacccacga agatataaga tccattcctt
    10081 tatatgagga atcttacatt ttattagcac ctaaagaaac atttaaaaat caaaattggg
    10141 tagatgttga aaatttgcca ctcatattgc caaacaaaaa ttctcaagtg cgcaaacact
    10201 tagatgacta ttttaataga agaaatattc gtccaaatgt cgttgtagaa acagatcgat
    10261 tcgaatcagc agttggattt gttcatcttg gcttaggtta cgctatcatt ccgagatttt
    10321 attaccaatc atttcacacg tccaatttag aatataaaaa aattcgtcca aacttatgcc
    10381 gaaaaattta tatcaattat cataaaaagc gtaaacactc cgaacaagta catacattcg
    10441 tacaacaatg ccaagattat ttatatggac ttttagaggc tctttaactt aagttattag
    10501 agcctcttat gcagttgctc agtcaactgt ataccttttg cctttaactt aagttattag
    10561 agcctcttat gcagctgctc agtcaactgt ataccttttg cctttaactt aagttattag
    10621 agcctcttat gcagttgctc agtcaactgt ataaaaatta atgacgtcat ttcaaaaatc
    10681 gatacaaaaa taatttatta taaaaattct aagaaagaag tgaacccgat gttaaaatct
    10741 attaatcata tatgcttttc agtcagaaat ttaaacgatt caatacattt ttatagagat
    10801 attttacttg gaaaattgct attgactggt aaaaaaactg cttattttaa gcttgcaggc
    10861 ctatggattg ctttaaatga agaaaaagat ataccacgta atgaaatcca cttttcatat
    10921 acacatatag ctttcactat agatgacagc gaatttaaat attggcatca gaggttaaaa
    10981 gataataacg tgaatatctt agaaggaaga gttagagata ttagagatag acaatcaatt
    11041 tactttactg accctgatgg tcataagcta gaattacata ctggcacact tgagaacaga
    11101 ttaaattatt ataaagaggc taaaccacat atgacatttt acaaataagg cgtcattata
    11161 aaaaggcctc ttgaactccg ttaaaatttt aattaattat tatataataa gagaactttt
    11221 caaacaatac agttgttatt tttactatct caacaaacat aaataagcag taagatgact
    11281 acaacttaag agtcttctta ctgcaattat ctttcaaata tatcaacgtt aatataactt
    11341 ctattacgaa atactcacat tctgccctgc aatgcaaatt tcgtcacata taaatatttt
    11401 taattatttt aaaaaatgat gcactaaatt agcaacgagc ttcgcagttc tattgtcagc
    11461 gtcatatgtt ggattcatct cagcaatact aactgaagac accttatcac ttggaataat
    11521 acgttttgcc aattcaagaa cagtatgtgg atacaaacct aacactgccg gcgcacttac
    11581 cccaggcgca aatgcactat caatgacatc catacaaatc gtaaacataa tgacatcatg
    11641 ttcatgtata aaacgctcaa tcatatcttt gattgttggt gatacgtgac tcaataattc
    11701 atctgcaaag acataatcaa tctttttctc tttagcataa tcaaataaac tttgcgtatt
    11761 cccaccttga gcaataccaa gcactaaata acctgtgttt tcatcttctt ctaaaatttg
    11821 tctaaagctc gttccagatg tagattgttg ttcagcacgt gtatcaaaat gcgcatcaat
    11881 atttatcaca ccaatagatt gtgttggata gactttacgc gttgctaaat attgagcata
    11941 cgcaatatca tgtccaccac ctaataaaaa cgtttgtcta tgattaacaa ttgacttcgc
    12001 tgcaagtgtt gcaaattctt tttgagtatc aattaattcc tcatgatcat gataaacatt
    12061 tccgtaatcg actaaagttt cacattgatt caaatccggc aaacctgcaa atgcttgttt
    12121 aatcgcatct ggtccttctt ttgcaccaat gcgccccttg tttaaagcaa cacctttgtc
    12181 aacagcatag cctaatatac caacccctga tggtgtacta cttttttcca atttcgacaa
    12241 atcttcaaat gtcactgttt gaaaatgcct gaattttttc gggtctgttt cactatctaa
    12301 ccttccagtc cataaatttg gttcaccttg cttgtacaca gcatttcccc ctcttattta
    12361 tgtggctttt taacaattaa agtataacgt actacaaatt ttgaattcaa ttcataatta
    12421 aaaagtactt atctatatac cttttacccc tattttctat tcactaacga acacgaaagc
    12481 gatttatgtt aataaaatac gtcaagacta ttacattttc attaatatta gcacagacaa
    12541 tttaactcac ggcttgtaat atgtataatt gttacaaaat gatattttgc ttgttaccta
    12601 atggaggtta catataatga aaaacaataa aatttctggt tttcaatggg caatgacgat
    12661 cttcgtcttt ttcgttatta caatggcgtt atccattatg cttagagatt tccagtctgt
    12721 cattggtgtc aaacacttta tatttgaagt tacagatcta gcaccattaa ttgctgcaat
    12781 catttgtata ctcgttttca aatataaaaa gatccaactt gcaggtttaa aattctcaat
    12841 cagcctgaaa gtaattgaac gtctactgct agctttaatt ttacctttaa ttattctaat
    12901 tattggtatg tacagcttta atacatttgc agatagcttt attttattac aatcaacagg
    12961 cttatcagta cctattacac atattctgat tggacatatt ctaatggcgt tcgtagtaga
    13021 attcggattc cgttcatact tacaaaatat tgttgaaacg aaaatgaata cgttctttgc
    13081 aagcatagtt gttggtttaa tgtactctgt attttcagca aatacaactt acgatacaga
    13141 atttgcagct tataatttcc tttacacatt ctcattctca atgattctag gtgagttaat
    13201 tagagctaca aaaggacgta cgatctacat cgcaacaaca tttcatgctt caatgacatt
    13261 tggccttatt ttcttattta gcgaagaaat cggtgattta ttttcaatta aggtcattgc
    13321 tatttcaaca gcaattgtag cggttggcta tattggttta agcttaatta ttcgaggtat
    13381 tgcttattta acaactagac gaaaccttga agaacttgag cctaataatt atttagacca
    13441 tgtcaatgac gatgaagaaa ctaatcatac tgaggctgaa aaatcttctt caaatattaa
    13501 agatgctgaa aaaacaggtg tagctactgc atcaacggtc ggtattgcta aaaatgatac
    13561 tgaaaatact gtggctgacg aaccaagcat tcatgaaggt actgaaaaaa cagaatctca
    13621 acatcacata gataatcaaa ctgaatctaa tcatgatgaa gatcatgaca tcacttcgaa
    13681 gtcagtagaa tcagcagaat cagttaaaca tgcaccacaa agtgacgatt taacaaacga
    13741 ttcaaatgaa gatgaaacag agcaatcatt aaaagaacct gcgacctata aagaagacag
    13801 acgttcatca gttgtaatcg atgcagaaaa acatatcgaa aaaactgaag aacaatcttc
    13861 agataaaaac aaataataac aaagcgcttg ctagtacctc ttaaaaagat gatgctagca
    13921 agcgcttttc tatactatat attatttttc taaaatttta acgccctctt gagtacctac
    13981 aataacttga tctgccatat ctaagaagta gcctgtctca aacacacctg tcagatgaat
    14041 taaatactca tggaacttat atgggtcaat tccttttggt aacttacaat ctaaaatata
    14101 attgccatta tcagttataa atgcgacatc ttcgtttaca cggcgttcta cttttatatc
    14161 agcgtatgat tcaattttac gtaatatgtg ataccagtta aatttatcca cctctactgg
    14221 taacttaaac gtctcaccta agtattgaac cattttcgtt tcatcgacaa ccacaacaaa
    14281 acgtgatgcc atttcatcta tcactttctc tctgaacagc gcaccaccac cacctttaat
    14341 tatatttaaa gatggatcta cttcatcagc accatcaatt gctaagtcga tatgatcaac
    14401 atcattgatt tcgcatattt taatacctag ttcttttgct aaaaatgcaa ttttattaga
    14461 agtgcataca cctgtaatat tgtaaccacg ttctttaatt agttgcgcca tttgaggtaa
    14521 gagtaattcc attgtacttc ctgtaccaat tcccagcgtc atgtcaccat tgatttgact
    14581 taaaacatca tttaatgtca ttaacttgag tgctttgaca tctttcatga aggtagcctc
    14641 ccatatttat gtaatctatt caattcatat tttacatgac tcgtataaat taacataccc
    14701 ttatttctaa cattttttta aacatatcaa tcagaattta caattctaaa aattttttat
    14761 agtacaatat actttaactt aacgttaagg agaatgatat gatatcaatt cacgcaattt
    14821 cgacaggaaa aatccaagat ttgccttata gctcgaaaag accgatgcgc tctgctttag
    14881 ataaaactaa gatttcacaa acaacatggt tatcttcaac tggtttcact ggtgatgaac
    14941 aggcttataa agatcatggt gggccacata aagcagtttg tgggtttagt aaacataact
    15001 atgcactgta tcaagatgat ttaccttcac tacctactca tgcgatgttt ggagagaatt
    15061 taacatttga ttatttagac gaatctgatg tttactttgg taatcaatat cgtttaggtg
    15121 aagcgttaat tgaggtttct gaaattagag aaccatgctg gaaaattcaa gcaaaatata
    15181 atattcctga tttagtgaag cgcatgtcta catctggtaa aacaggtttc tatttccggg
    15241 tattaaaaca aggctatgta tctccaaatg atcagcttta cttaatacaa gaagcaccaa
    15301 tcgaacatcg tttatctgta caacagctta atgaccttta ttataatgat agacaaaatc
    15361 aagatatgtt acgatatgca ctaaacaatc catttctgtc accaacaaga cgcgataaac
    15421 ttcaaaaaat gtataacaga acattgaaat aattaccttt cattataaat gttaaatgaa
    15481 cttttcaaaa caaaaaggaa tcaacttaca catcgtttgt ataatagtct tatctataac
    15541 tactgatgct attgattcct tttttatgtt atatattttc tttatttcaa actgtatatt
    15601 aatgatgtca cttcatttga tacgattctt gataacctat tcaaaat
//
LOCUS       DATTQF010000052        15478 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.052, whole genome shotgun sequence.
ACCESSION   DATTQF010000052 DATTQF010000000
VERSION     DATTQF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.052"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            115..963
                     /locus_tag="VB241_001371"
     CDS             115..963
                     /locus_tag="VB241_001371"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEP1244669.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFNIGASLEELALLGSKIGTDIPFCIYNKTALCTGKGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLAQKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            977..1801
                     /gene="purR"
                     /locus_tag="VB241_001372"
     CDS             977..1801
                     /gene="purR"
                     /locus_tag="VB241_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP1244670.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1818..2198
                     /locus_tag="VB241_001373"
     CDS             1818..2198
                     /locus_tag="VB241_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP1244671.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            2270..2572
                     /gene="spoVG"
                     /locus_tag="VB241_001374"
     CDS             2270..2572
                     /gene="spoVG"
                     /locus_tag="VB241_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEP1244672.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDEIDEVVPDKNATSEDSEEA"
     gene            2761..2892
                     /locus_tag="VB241_001375"
     CDS             2761..2892
                     /locus_tag="VB241_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244673.1"
                     /translation="MRLNDACIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            2915..4267
                     /gene="glmU"
                     /locus_tag="VB241_001376"
     CDS             2915..4267
                     /gene="glmU"
                     /locus_tag="VB241_001376"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP1244674.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAENAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            4414..5379
                     /locus_tag="VB241_001377"
     CDS             4414..5379
                     /locus_tag="VB241_001377"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP1244675.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            5529..6182
                     /locus_tag="VB241_001378"
     CDS             5529..6182
                     /locus_tag="VB241_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEP1244676.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            6493..7065
                     /locus_tag="VB241_001379"
     CDS             6493..7065
                     /locus_tag="VB241_001379"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP1244677.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            7065..10571
                     /gene="mfd"
                     /locus_tag="VB241_001380"
     CDS             7065..10571
                     /gene="mfd"
                     /locus_tag="VB241_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP1244678.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFVDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNVIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQIHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            10561..12087
                     /locus_tag="VB241_001381"
     CDS             10561..12087
                     /locus_tag="VB241_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP1244679.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGMLLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLVELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            12087..13280
                     /locus_tag="VB241_001382"
     CDS             12087..13280
                     /locus_tag="VB241_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEP1244680.1"
                     /translation="MEHTITIVGLGNYGIDDLPLGIYKFLMTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSSMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKITTPTYLYHDFDFATEVIDTLVDENKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            13277..13540
                     /locus_tag="VB241_001383"
     CDS             13277..13540
                     /locus_tag="VB241_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEP1244681.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            13558..13950
                     /locus_tag="VB241_001384"
     CDS             13558..13950
                     /locus_tag="VB241_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP1244682.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            14055..14456
                     /locus_tag="VB241_001385"
     CDS             14055..14456
                     /locus_tag="VB241_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEP1244683.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            14636..>15478
                     /gene="tilS"
                     /locus_tag="VB241_001386"
     CDS             14636..>15478
                     /gene="tilS"
                     /locus_tag="VB241_001386"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP1244684.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLNRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRNGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISA"
BASE COUNT         5554 a         2103 c         3131 g         4690 t
ORIGIN      
        1 tggagaaaat tactttttta ttaaaaaaac actaaaaaac aaattaaaat gtcaaatatt
       61 aattctcttt atgttaaaat catcatatta agataacgaa aagagggcgc aaaaatgata
      121 tatgaaacgg caccagccaa aattaatttt acgctcgata cactttttaa aagaaatgat
      181 ggctatcatg agattgaaat gataatgaca acagttgatt taaatgatcg tttaactttt
      241 cataaaagaa aagatcgaaa gatagttgtt gagattgaac ataattatgt gccttctaat
      301 cataaaaatc tcgcatatcg tgcagcgcaa ctatttattg agcaatatca actaaagcaa
      361 ggtgtaacaa tttctatcga taaagaaata cctgtttctg ctggcttagc tggaggttcg
      421 gctgatgcag cagcaacgtt aagaggattg aatcgacttt ttaatatagg ggcgagtttg
      481 gaagaattgg ctctactagg cagtaaaatc gggacagata ttccgttttg tatttataat
      541 aaaactgcac tatgtactgg aaaaggagaa aaaatcgagt ttttaaataa accaccttca
      601 gcttgggtga ttcttgctaa accaaactta ggcatatcat caccagatat atttaagttg
      661 attaatttag ataagcgtta cgacgtacat acgaaaatgt gttatgaggc cttagaaaat
      721 cgagattatc aacaattatg tcaaagtttg tctaatcgat tagagccaat ttctgtttca
      781 aaacacccac aaatcgataa attaaaaaac aatatgttga aaagtggtgc agatggtgcg
      841 ttaatgagtg gaagcggacc gactgtgtat gggctagcac aaaaagaaag ccaagcaaaa
      901 aatatttata atgcagttaa cggttgttgt aatgaagtgt acttagttag actattagga
      961 tagaagggtt gaaaagatga gatataaacg aagcgagaga attgttttta tgacgcaata
     1021 tttgatgaac catccgaata aattgattcc attaactttt tttgtgaaaa aatttaaaca
     1081 ggcgaagtct tcaataagtg aagatgtcca aattataaaa aatacattcc aaaaagaaaa
     1141 gttaggtaca gtaattacta ctgctggcgc aagtggtggt gttacgtata aaccaatgat
     1201 gagtaaagaa gaggcgactg aagttgttaa tgaggtcatt actctattag aagagaaaga
     1261 acgtttgtta cctggcgggt atttattttt atcagatttg gtaggtaatc catcgctact
     1321 aaacaaagtt ggtaagttaa ttgccagtat ttacatggaa gaaaaattag atgctgttgt
     1381 taccattgcg acaaaaggta tttcattggc aaatgcggtt gctaatattt taaatttacc
     1441 agtagtagtg attagaaaag acaacaaggt gactgaaggt tctacagttt caattaatta
     1501 cgtttcagga tcttcaagaa aaatagagac aatggtactt tcgaagagaa ctttagcaga
     1561 aaattcaaat gttttagttg tcgatgattt tatgagggct ggtggctcta tcaatggtgt
     1621 tatgaattta atgaatgagt ttaaagccca tgtaaaaggg gtatcagtac ttgtagaatc
     1681 aaaagaagtt aaacaaagat tgattgaaga ttatacttcc ttagtgaaat tatctgatgt
     1741 agatgaatat aatcaagagt ttaacgtaga acctggcaac agtttatcta agttttcata
     1801 aaaggagttt tagtattatg aaaatcatta acacaacaag attaccggaa gcacttggac
     1861 catattcgca tgcaacagtt gtgaatggta tggtttatac ttctggtcaa attccattga
     1921 atgttgatgg gaaaatcgta agcgctgatg ttcaagcaca aacaaaacaa gttttagaaa
     1981 atttaaaggt tgttttggaa gaagcaggat ctgatttgaa ttctgttgcg aaagcgacca
     2041 ttttcattaa agatatgaat gatttccaaa aaataaatga agtgtatggt caatatttta
     2101 atgaacacaa gccagcgcgt agttgtgtag aggttgcgcg tttgccaaaa gatgtgaaag
     2161 tagaaattga attagtaagt aaaattaagg aattataatt ttcgattaat atgtttaatc
     2221 aagcttctaa ataaaacaga gagagatata ctataggggg gctcactaca tgaaagtgac
     2281 agatgtaaga cttagaaaaa tacaaacaga tggacgaatg aaagcactcg tttccattac
     2341 attagatgaa gctttcgtaa ttcatgattt acgtgtaatt gaaggaaact ctggcttgtt
     2401 cgttgcaatg ccaagtaaac gtacaccaga tggtgaattc cgcgacatcg cgcatcctat
     2461 taattcagat atgagacaag aaattcaaga tgcagtgatg aaagtatatg atgaaataga
     2521 tgaagtagta ccagataaaa acgctacatc agaagattca gaagaagctt aatcaatttt
     2581 atatttagcg atgtaataca tttgcaataa gttgatttga tactgtcgat aaagcataaa
     2641 gctttgtcgg cagttttttt agtttgtatt aatattaatt ttatgaaatg aaaggctaat
     2701 aaatatatat gttaacagat tatgatgata tgaaaattat tgatacattg ctgataaaaa
     2761 ttgcgtttga atgatgcttg tatttttgaa gtaaggaaaa agttgttttt aaaattacaa
     2821 cgaattaaaa acaatgcctt ttatatgttg aaagagtatt gcagattaaa ttataataat
     2881 gacgaagtgt aaaatttaat gggggttaat gttcatgcga agacacgcga taattttggc
     2941 agcaggtaaa ggcacaagaa tgaaatctaa aaagtataaa gtgctacacg aggttgctgg
     3001 aaaacctatg gtcgaacatg tattggaaag tgtgaaaggc tctggtgtcg atcaagttgt
     3061 aaccatcgta ggacatggtg ctgaaagtgt aaaaggacat ttaggcgagc gttctttata
     3121 cagttttcaa gaggaacaac tcggtactgc gcatgcagtg caaatggcga aatcacactt
     3181 agaagacaag gaaggtacga cgatcgttgt atgtggtgac acaccgctca tcacaaagga
     3241 aacattagaa acattgattg cgcatcacga ggatgctaat gctcaagcaa ctgtattatc
     3301 tgcatcgatt caacaaccat atggatacgg aagaatcgtt cgaaatgcgt caggtcgttt
     3361 agaacgcata gttgaagaga aagatgcaac gcaagctgaa aaggatatta atgaaattag
     3421 ttcaggtatt tttgcgttta ataataaaac gttgtttgaa aaattaacac aagtgaaaaa
     3481 tgataatgcg caaggtgaat attacctccc tgatgtattg tcgttaattt taaatgatgg
     3541 cggcatcgta gaagtctatc gtaccaatga tgttgaagaa atcatgggtg taaatgatcg
     3601 tgtaatgctt agtcaggctg agaatgcgat gcaacgtcgt acgaatcatt atcacatgct
     3661 aaatggtgtg acaatcatcg atcctgacag cacttatatt ggtccagacg ttacaattgg
     3721 tagtgataca gtcattgaac caggcgtacg aattaatggt cgtacagaaa ttggcgaaga
     3781 tgttgttatt ggtcagtact ctgaaattaa caatagtacg attgaaaatg gtgcatgtat
     3841 tcaacagtct gttgttaatg atgctagcgt aggagcgaat actaaggtcg gaccgtttgc
     3901 gcaattgaga ccaggcgcgc aattaggtgc agatgttaag gttggaaatt ttgtagaaat
     3961 taaaaaagca gatcttaaag atggtgccaa ggtttcacat ttaagttata ttggcgatgc
     4021 tgtaattggc gaacgtacta atattggttg cggaacgatt acagttaact atgatggtga
     4081 aaataaattt aaaactatcg tcggcaaaga ttcatttgta ggttgcaatg ttaatttagt
     4141 agcacctgta acaattggtg atgatgtatt ggtggcagct ggttccacaa tcacagatga
     4201 cgtaccaaat gacagtttag ctgtggcaag agcaagacaa acaacaaaag aaggatatag
     4261 gaaataatca tttacgtatt taaaatggct aggataaaag gataatccta tgtaatatta
     4321 atgtaatctt tatgatttaa tgattcgcat agtaatggag ttacatttta tatataatag
     4381 taattgcgta agtaaataat tggaggacta taaatgttaa ataatgaata taagaattcg
     4441 tcattaaaga ttttttcatt gaaaggaaac gaagcattag cgcaagaagt tgctgaccaa
     4501 gtaggaattg aactaggtaa atgttcagtt aaacgtttta gtgatggaga aattcaaatt
     4561 aatatcgaag agagtattcg tggttgtgac gtatttatta ttcaaccaac atcatatcct
     4621 gtgaatctac atttaatgga attattaatt atgattgatg cttgtaaacg tgcttctgca
     4681 gcaacaatca atattgtagt gccatattat ggatatgcaa gacaagatag aaaagcccgt
     4741 agccgtgagc caatcactgc taaattagtt gcaaacttaa tcgaaacagc tggcgcaact
     4801 cgtatgattg cgttagactt acatgcacca caaattcaag gattctttga tattccaatt
     4861 gaccacttaa tgggtgtgcc aattcttgct aaacatttca aagatgatcc gaatattaac
     4921 ccagaagaat gtgtcgttgt ttcaccagac catggcggtg ttacacgtgc acgtaaatta
     4981 gctgacattt taaaaactcc aattgcaatt atagataaac gtcgtcctag accaaatgtt
     5041 gctgaagtga tgaacattgt tggtgagatt gaaggacgta cggcaattat tattgacgat
     5101 attattgata cagcaggtac aatcacttta gctgcacaag cattaaaaga taaaggtgct
     5161 aaagaagtat atgcttgttg tacacaccct gttttatcag gaccggctaa agaacgtatc
     5221 gaaaattctg ctataaaaga attaatcgta acaaactcaa ttcatttaga tgaagatcgc
     5281 aaaccatcta acactaaaga attatctgtt gctggtttaa tcgcacaagc tatcattcgt
     5341 gtatacgaaa gagaatcagt tagcgtatta tttgactaat atttaaaagg cgtttgacga
     5401 acatattcca aacgtgtata atagtttcgt tcgtgattat acgaataaat aaacacttgc
     5461 aagcaacgat gatgttgatg ggtaagtgag gtgctcgttt tgagcaaaaa tgaaaggtgg
     5521 aaatgagaat ggcttcatta aagtcaatca tccgtcaagg taaacaaaca cgttcagatc
     5581 ttaaacaatt aagaaaatct ggtaaagtac cagcagtagt atacggttac ggtactaaaa
     5641 acgtgtcagt taaagttgat gaagtagaat tcatcaaagt tatccgtgaa gtaggtcgta
     5701 acggtgttat cgaattaggc gttggttcta aaactatcaa agttatggtt gcagactacc
     5761 aattcgatcc acttaaaaac caaattactc acattgactt cttagcaatc aatatgagtg
     5821 aagaacgtac tgttgaagta ccagttcaat tagttggtga agcagtaggc gctaaagaag
     5881 gcggcgtagt tgaacaacca ttattcaact tagaagtaac tgctactcca gacaatattc
     5941 cagaagcaat cgaagtagac attactgaat taaacattaa cgacagctta actgttgctg
     6001 atgttaaagt aactggcgac ttcaaaatcg aaaacgattc agctgaatca gtagtaacag
     6061 tagttgctcc aactgaagaa ccaactgaag aagaaatcga agctatggaa ggcgaacaac
     6121 aaactgaaga accagaagtt gttggcgaaa gcaaagaaga cgaagagaaa actgaagagt
     6181 aagtttaact gattaccata aagtttttat actttgttaa acaagcactg tgcttatttt
     6241 aatataagca tggtgctttt tgtgttatta taaagcttaa ttaaacttta ttgctttgta
     6301 ctaaagttaa attaatttta gtgagtaaaa gacattaaac tcaacaatga tacatcataa
     6361 aaattttaat gtacttgatt ttaaaataca tacttactaa tctaaagaat aatgataatt
     6421 gagggcaatg gcggaaaata gatgttgtca ttataataat aaatgaaaca attatgttgg
     6481 aggtaaacac gcatgaaatg tattgtaggt ctaggtaata taggtaaacg ttttgaactt
     6541 acaagacata atatcggctt tgaagtcgtt gattatattt tagagaaaaa taatttttca
     6601 ttagataaac aaaagtttaa aggtgcatat acaattgaac gaatgaacgg agataaagtg
     6661 ttattcatcg aaccaatgac aatgatgaat ttgtcaggtg aagcagttgc accgattatg
     6721 gattattaca atgttaatcc agaagattta attgtcttat atgatgattt ggatttagaa
     6781 caaggacaag ttcgcttaag acaaaaagga agtgcgggcg gtcacaatgg tatgaaatca
     6841 attattaaaa tgcttggtac agaccaattt aaacgtattc gtattggtgt gggaagacca
     6901 acgaatggta tgacggtacc tgattatgtt ttacaacgct tttcaaatga tgaaatggta
     6961 acgatggaaa aagttatcga acacgcagca cgcgcaattg aaaagtttgt tgaaacatca
     7021 cgatttgacc atgttatgaa tgaatttaat ggtgaagtga aataatgaca atattgacaa
     7081 cgcttataaa agaagataat cattttcaag accttaatca ggtatttgga caagcaaaca
     7141 cactagtaac tggtctttcc ccgtcagcta aagtgacgat gattgctgaa aaatatgcac
     7201 aaagtaatca acagttatta ttaattacca ataatttata ccaagcagat aaattagaaa
     7261 cagatttact tcaatttgta gatgctgaag aattgtataa gtatcctgtg caagatatta
     7321 tgaccgaaga gttttcaaca caaagccctc aactgatgag tgaacgtatt agaactttaa
     7381 ctgcgttagc tcaaggtaag aaagggttat ttatcgttcc tttaaatggt ttgaaaaagt
     7441 ggttaactcc tgttgaaatg tggcaaaatc accaaatgac attgcgtgtt ggtgaggata
     7501 tcgatgtgga ccaatttctt aacaaattag ttaatatggg gtacaagcgg gaatctgtgg
     7561 tatcgcatat tggtgaattc tcattgcgag gaggtattat cgatatcttt ccgctaattg
     7621 gggaaccaat cagaattgag ctatttgata ccgaaattga ttctattcgg gattttgatg
     7681 ttgaaacgca gcgttccaaa gataatattg aagaagtcga tatcacaact gcaagtgatt
     7741 atatcattac tgaagaagta atcagccatc ttaaagaaga gttaaaaact gcatatgaaa
     7801 atacaagacc caaaatagat aaatcagtgc gcaatgatct gaaagaaacg tatgaaagct
     7861 ttaaattatt cgaaagtaca tactttgatc atcaaatact acgtcgttta gtagcgttta
     7921 tgtatgaaac accttcgaca attattgatt atttccaaaa agatgcaatc attgcggttg
     7981 atgaatttaa tcgtattaaa gaaactgaag aaagtttaac agtagagtct gattcgttta
     8041 ttagcaacgt tattgaaagt ggtaatggat ttataggaca aagttttata aaatatgatg
     8101 attttgaaac attgattgaa ggctatcctg tcacttattt ttcattattc gctacaacaa
     8161 tgccgataaa actaaatcat attattaaat tttcatgtaa acctgtccaa caattttatg
     8221 ggcaatatga cattatgcgt tctgaatttc aacgatatgt taatcaaaat tatcatatcg
     8281 tggttttggt cgaaaccgaa actaaagttg aacgtatgca agcgatgtta agtgaaatgc
     8341 atattccatc aataacaaaa ttgcatcgct caatgtcatc ggggcaagca gtgattattg
     8401 aaggcagttt atctgaagga tttgaactac ctgatatggg attagttgtc attactgaac
     8461 gtgagctttt taaatcaaaa cagaaaaagc aacgaaaacg tacgaaagct atctcaaatg
     8521 ctgaaaaaat taagtcttac caagatttaa atgtgggaga ttatattgtt catgtgcatc
     8581 atggtgttgg tagatattta ggtgttgaga cgctcgaagt agggcaaata catcgtgatt
     8641 atattaaatt gcaatataaa ggtacggatc aactatttgt tccagtagat caaatggatc
     8701 aagttcaaaa atatgtagct tcggaagata agacgccaaa attaaataaa ctcggtggca
     8761 gtgaatggaa aaaaacaaaa gctaaagttc aacaaagtgt tgaagatatt gctgaagagt
     8821 tgattgattt atataaagaa agagaaatgg cagaaggtta tcaatatggg gaagacacag
     8881 ctgagcaaac aacatttgaa ttagattttc catatgaact tacgcctgac caagcgaaat
     8941 ctatcgatga aattaaagat gacatgcaaa aatcgcgtcc aatggatcgc ttgctatgtg
     9001 gtgatgttgg ttatggtaaa actgaagttg cagtgagagc agcattcaaa gctgtaatgg
     9061 aaggaaagca ggttgcattt ttagttccta caactatttt agctcagcaa cattatgaga
     9121 cgttaattga gcgtatgcaa gattttcctg ttgaaattca attaatgagt cgttttagaa
     9181 cgccaaaaga gataaaacaa actaaggaag gacttaaaac tgggtttgtt gacatagttg
     9241 ttggtacaca caaattactt agtaaagata tacagtataa agatttaggg ctgttgattg
     9301 tagatgaaga acaacgattt ggtgtacgcc ataaagagcg tattaaaaca ttaaaacata
     9361 atgtagatgt actaacattg actgcaaccc caatacctag aacattgcat atgagtatgc
     9421 taggtgtgcg cgatttgtca gtgattgaaa cgccgccaga aaatcgtttc ccagttcaaa
     9481 catatgtatt agaacagaac atgagtttta tcaaagaagc tttagaaaga gaactatccc
     9541 gtgatggcca agtattttat ctttataata aagtgcaatc catttatgaa aaacgagaac
     9601 aactccagat gttaatgcca gatgctaaca ttgcagttgc tcatggacaa atgacagagc
     9661 gcgatttaga agaaacgatg ttaagtttta tcaataatga atatgatatt ttagtaacga
     9721 cgacgattat tgaaacaggt gtcgatgtcc caaatgcaaa tactttgatc attgaagatg
     9781 cagatcgctt tggattgagt cagttgtatc aattaagagg tcgtgttggt cgttcaagtc
     9841 gtattggtta tgcatacttc ttacatccag caaataaggt actaactgag actgcagaag
     9901 atcgattaca agcgattaaa gaatttacgg agttaggctc aggatttaag attgcgatgc
     9961 gtgatttgaa cattcgtggt gctggtaatt tgttaggtaa acaacagcac ggctttattg
    10021 atacagttgg atttgatttg tacagtcaaa tgttagaaga agctgtaaat gaaaaacgtg
    10081 gtattaagga accagaatct gaggtgccag aagtcgaagt tgatttaaac ttggatgcat
    10141 atttgccaac agaatatatt gcaaatgaac aagctaaaat tgaaatttat aaaaagctaa
    10201 gaaaaactga aacatttgat caaattatcg acataaaaga tgaattaatt gatcgtttca
    10261 atgattatcc tgttgaagta gcacgtttgc ttgatatagt ggaaataaaa gtacacgcat
    10321 tacattcagg tatcacgttg attaaagata aagggaaaat aattgatatt catttatctg
    10381 taaaagccac tgaaaatatt gatggcgaag tgctgttcaa agcaacacaa cctttaggta
    10441 gaacaatgaa ggttggtgtt caaaataatg caatgacaat tactttaacg aaacaaaatc
    10501 aatggcttga tagtttgaag tttttagtta agtgcattga agaaagtatg agaatcagtg
    10561 atgaagcata aagaagcatt taatggcgtt gtcgtgttaa ctgctgcatt aattgtcatt
    10621 aaaattctga gtgctgtata tcgaattcca tatcaaaata tattaggcga tacaggtttg
    10681 tatgcatatc aacaagtgta tccaattgta gcattaggaa tgatattatc gatgaatgcc
    10741 attcctagtg caattacaca aaatataggg aagtatcata gtgacgaagc atatgcaaaa
    10801 gcagtcgctt atatacaatt agttggtatg ttattattta ttgctatttt tgtgtttgcg
    10861 aacaatattg cacatatgat gggtgatagc catttaacac ctatgattca agctgcaagt
    10921 ttaagcttta tatttatagg tatgcttggc gtgttaagag gttattatca atctgcaaat
    10981 aatatgacag ttccggctat ttcccaggtt atagaacaag ttatacgagt aggtattatc
    11041 attgttacta ttgttatttt tgtagataga ggttggacga tatatgaagc gggtacaatt
    11101 gctattttag catcaacgat aggtttttta ggttcttcaa tttatttagt agcgcaccga
    11161 ccttttaagt ttaaaatggt aaataacact gcaaagattg tttggaaaca gttcgcactt
    11221 tcggttttga ttttcgctat cagtcaatta atcgtaattt tatggcaagt gattgatagt
    11281 gttactatta ttaagtcact tcaagcgata cgcgtgccat tcgatgttgc tataactgaa
    11341 aaaggagtct atgaccgtgg tgcatcattt attcagatgg gattgattgt aactacaaca
    11401 tttagttttg cgctcattcc tctgttaagt gacgcaatca aaatgaataa tcaggtactt
    11461 atgaatcgtt atgcaaatgc gtcattaaag attacgattt taataagtac agcagcggga
    11521 ataggattaa ttaatttatt gcctttaatg aacggtgtat tttttaagac gaatgattta
    11581 accttaacgt taagcgttta tatgattacg gttatttgtg tatcgttaat tatgatggat
    11641 atggcattat tacaagctca acatgctgtg agaccaattt ttgtcggtat gacggcagga
    11701 ttggttatta aatttatact taatatcatt ttgattcgtt taagtggcat tattggtgcg
    11761 agcattagta ctgttgtatc attaattata ttcggtacga ttatccatat tgctgtcacg
    11821 agaaaatacc atttacatgc gatgagacga ttttttatca atgttgtttt aggtatggta
    11881 tttatgtcga ttgttgttca atgcgtgtta aacatagtga caacacacgg tagaatcact
    11941 ggactcgttg aattattatg tgcagcagta ttaggtatca ttgcattgtt tttctatatt
    12001 tttagattta atgttttgac atataaagag ttaacttatt taccatttgg ttcaaagttg
    12061 tatcaaatta agaaaggaag acgttgatgg aacataccat tacgattgtt ggcttaggaa
    12121 actatggcat tgatgatttg ccgctaggga tatataaatt tttaatgaca caagataaag
    12181 tttatgcaag aacgttagat catccagtta tagaatcgtt gcaagatgaa ttaacatttc
    12241 agagttttga ccatgtttat gaagcacatg accaatttga agatgtctat aatgatattg
    12301 tggcgcaatt ggttgaagct gctaatgaaa aagatattgt ctatgcggtt ccgggtcatc
    12361 ctagagttgc tgagacaact acagtgaaat tactggcttt agcaaaggac aatactgata
    12421 tagatgtgaa agttttaggt gggaaaagct ttattgatga tgtgtttgaa gcagttaatg
    12481 tagatccaaa tgatggcttc acactgttag atgcgacatc attacaagaa gtaacactta
    12541 atgttagaac gcatacattg attacgcaag tttatagttc aatggttgct gctaatttga
    12601 aaatcacttt aatggaacga tatcctgatg attatcctgt tcaaattgtc actggtgcac
    12661 gaagcgatgg tgcggataac gttgtgacat gcccattata tgaattggat catgatgaaa
    12721 atgcattcaa taatttgacg agtgtattcg taccaaaaat cacaacaccg acatatttgt
    12781 atcatgactt tgattttgca acggaagtga ttgatacttt agttgatgaa aataaaggtt
    12841 gtccatggga taaagtgcaa acgcatgaaa cacttaagcg ttatttactt gaagaaacat
    12901 tcgaattgtt cgaagctatt gacaatgaag atgattggca tatgattgaa gaactaggag
    12961 atattttatt acaagtgtta ttgcatacta gtattggtaa aaaagaaggg tatatcgaca
    13021 ttaaagaagt gattacaagt cttaatgcta aaatgattcg tagacaccca cacatatttg
    13081 gtgatgctaa tgctgaaact atcgatgact taaaagaaat ttggtctaag gcgaaagatg
    13141 ctgaaggtaa acagccaaga gttaaatttg aaaaagtatt tgcagagcat tttttaaatt
    13201 tatatgagaa gacgaaggat aagtcatttg atgaggccgc gttaaagcag tggctagaaa
    13261 aaggggagag taatacatga gattagataa atatttaaaa gtatcacggt taataaagcg
    13321 acgtacgcta gcaaaagagg taagtgatca aggtagaatt acaataaatg gtaatgttgc
    13381 taaagctgga tcggatgtta aagttgaaga tgtgctgacg attcgctttg gtcaaaaatt
    13441 agtaacagtt aaagtaactg cattaaatga acatgcatct aaagataacg cgaagggcat
    13501 gtatgaaatc attgaagagc gtcgacttga agaagcgtaa attggaggtg acaagcaatg
    13561 aaaaataaag tagaacatat agaaaatcag tacacgtcgc aagagaacaa gaaaaaacaa
    13621 cgtcaaaaaa tgaaaatgcg tgttgttcgt aggcgtatta cagtatttgc gggtgtatta
    13681 cttgcgataa ttgttgtttt atcaatcttg cttgttgtcc aaaaacatcg caatgatatt
    13741 gatgcacagg agcgaaaagc gaaagaagca cagtttcaaa agcaacaaaa tgaagaaatt
    13801 gcgttaaaag aaaagttgaa taatctgaat gacaaagatt atattgaaaa aattgcgcgt
    13861 gatgattatt acttaagcaa caaaggtgaa gtgattttta ggttgccaga agacaaagat
    13921 tcgtctagct caaaatcttc gaaaaaataa atccaaattg attcaaaatt atccgagtat
    13981 agacattgtg aaaaaatcca aacaaggata taataaggga aaatcgaatc aaatcgggag
    14041 gatttattta acatatgtca atcgaagttg gaaataagct taaaggtaaa gtcactggta
    14101 ttaaaaagtt tggtgcattc gtagaattac ctgaaggaaa aagtggttta gttcacatta
    14161 gtgaagtcgc agataattat gttgaaaacg tagaagagca cctttctgtt ggtgatgaag
    14221 tagacgtaaa agtattatct attgctgatg atggaaaaat tagtctttca ataaagaaag
    14281 ctaaagaccg tccacgtaga caacatacga gtaaaccaag tcatcaaaaa ccagtgcaaa
    14341 aagccgaaga ttttgaaaag aaattaagca atttcttaaa agatagtgaa gataaattaa
    14401 cttcaatcaa acgtcaaaca gaatctagac gcggtggcaa aggttctaga cgttaattaa
    14461 aataaataaa gactgtttcg ataaggaata tatttagaat gatgcgtatc gaataatcga
    14521 ttgcagcgtt agacaatcta agactgtttc ttaaataagg agcagtctct tttatttgta
    14581 atgatataac taagacttat accatttttg aaaattgtaa aagtgaggtg atgttatgca
    14641 gttaaatagt aatggttggc atgttgatga ccatattgtt gtcgctgttt ctacaggtat
    14701 tgatagtatg tgtttattgt atcaactact aaatgattat aaagatagtt atagaaaact
    14761 aacatgttta catgtcaatc atggcgttag gtcagcttca atcgaggaag ccagattttt
    14821 agaagcatac tgcgaacgtc atcacatcga tttacatatc aaaaagttgg atttgtcgca
    14881 tagtctcaat cgaaataaca gcattcagaa tgaagctcga attaaacgtt acgaatggtt
    14941 tgatgaaatg atgaatgtat tagaagcgga tgtattgcta acggcgcatc atttggacga
    15001 tcaattagaa actattatgt atcgcatttt taatgggaaa tcaacgcgta ataaactagg
    15061 atttgatgag ttgtcgaagc gaaatggtta tcagatttat cgaccacttt tagctgtctc
    15121 taaaaaagaa ataaaacaat tccaagagag atatcatata ccatattttg aagatgaatc
    15181 taataaagat aacaaatatg ttagaaatga tattcgtaat agaattattc cagctattga
    15241 tgaaaataat caacttaaag tatcgcattt attaaaatta aaacaatggc atgatgaaca
    15301 atatgatatt ttgcaatatt cagctaaaca atttattcaa gaatttgtga agtttgatga
    15361 acagtcaaaa tatttagagg tttctaggca agcttttaat aacttgccaa actcattaaa
    15421 gatggttgtg ttggattgcc tattatcaaa gtattatgag ttgtttaata ttagtgct
//
LOCUS       DATTQF010000053        15179 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.053, whole genome shotgun sequence.
ACCESSION   DATTQF010000053 DATTQF010000000
VERSION     DATTQF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.053"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..410
                     /locus_tag="VB241_001387"
     CDS             <1..410
                     /locus_tag="VB241_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HEP1244685.1"
                     /translation="QYTGFTKKAINKTQNNGYENEYFYIVANIPTLQEYRKYYEPLIK
                     KNNLNFKKGMKEARKGVGYKAEIEVHTTLFSKSSNFSKDKKLDDVIDLSESTKKLHLN
                     FENTKIFLQLAKSTISTNRVNYSDNESIRIEVE"
     gene            468..1376
                     /locus_tag="VB241_001388"
     CDS             468..1376
                     /locus_tag="VB241_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HEP1244686.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSNSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLYKFFK
                     DNEKKYQYTGFTKEAINKTQNSGYENEYFYITTIPYNLTEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKLSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKEKEIPNGIDLSI
                     KFSDNKINTVKPNYNGKSTSEYGVFD"
     gene            1581..1745
                     /locus_tag="VB241_001389"
     CDS             1581..1745
                     /locus_tag="VB241_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244687.1"
                     /translation="MTILTQLSDRIKKTIPTTDKTTNQSNLPNQSTQITNQNYPNNAR
                     KSPMKHSPIT"
     regulatory      1828..1962
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2024..2569
                     /locus_tag="VB241_001390"
     CDS             2024..2569
                     /locus_tag="VB241_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP1244688.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(2675..2923)
                     /locus_tag="VB241_001391"
     CDS             complement(2675..2923)
                     /locus_tag="VB241_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP1244689.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            3031..3984
                     /locus_tag="VB241_001392"
     CDS             3031..3984
                     /locus_tag="VB241_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1244690.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLENITEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEFEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTIEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            3974..5353
                     /locus_tag="VB241_001393"
     CDS             3974..5353
                     /locus_tag="VB241_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEP1244691.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLEIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQVFKKSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            5506..6966
                     /gene="ebpS"
                     /locus_tag="VB241_001394"
     CDS             5506..6966
                     /gene="ebpS"
                     /locus_tag="VB241_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HEP1244692.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEGYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSGKVNNSTEDKASEDKSKEHHNGKKGAAIGAGTAGLA
                     GGAASNSASAASKPHASNNASQNNDEHDHHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            7104..7190
                     /locus_tag="VB241_001395"
     CDS             7104..7190
                     /locus_tag="VB241_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244693.1"
                     /translation="MAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            7351..8337
                     /locus_tag="VB241_001396"
     CDS             7351..8337
                     /locus_tag="VB241_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEP1244694.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(8452..9420)
                     /locus_tag="VB241_001397"
     CDS             complement(8452..9420)
                     /locus_tag="VB241_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP1244695.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISLHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNEKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNMPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            9497..10156
                     /locus_tag="VB241_001398"
     CDS             9497..10156
                     /locus_tag="VB241_001398"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP1244696.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKTIEEVTDEILAMVSQI
                     K"
     gene            10187..10303
                     /locus_tag="VB241_001399"
     CDS             10187..10303
                     /locus_tag="VB241_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244697.1"
                     /translation="MQRLMHIQIECADNDLLNLKGDFYSTMNESCLFTFGKS"
     gene            10420..10611
                     /locus_tag="VB241_001400"
     CDS             10420..10611
                     /locus_tag="VB241_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244698.1"
                     /translation="MSVTNLRIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            10868..12043
                     /gene="rpsA"
                     /locus_tag="VB241_001401"
     CDS             10868..12043
                     /gene="rpsA"
                     /locus_tag="VB241_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP1244699.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDDIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            12265..13575
                     /gene="der"
                     /locus_tag="VB241_001402"
     CDS             12265..13575
                     /gene="der"
                     /locus_tag="VB241_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP1244700.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            13592..14590
                     /locus_tag="VB241_001403"
     CDS             13592..14590
                     /locus_tag="VB241_001403"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP1244701.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQEAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQYADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKN
                     VSKLTQDLFMNDYLRVYTNDDLVGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            14761..15033
                     /locus_tag="VB241_001404"
     CDS             14761..15033
                     /locus_tag="VB241_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP1244702.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         5674 a         2215 c         2717 g         4573 t
ORIGIN      
        1 atcaatatac aggatttaca aaaaaggcaa ttaacaagac acaaaataat ggctatgaaa
       61 atgaatattt ttatatagtt gcaaatatac cgacgctcca agaatataga aaatattacg
      121 aacccctaat aaagaagaat aatctgaatt ttaaaaaagg tatgaaagaa gcaaggaaag
      181 gagtaggcta taaagctgaa atagaagtac atactacatt gttttcgaaa agtagtaact
      241 tttcaaagga caaaaaatta gatgatgtta tagatttgtc tgaaagtacg aaaaagttac
      301 accttaattt tgaaaatacg aaaatatttt tacaactagc aaaatctact attagtacta
      361 atcgagttaa ttacagtgat aatgagtcta taaggattga ggtcgaatga cttgaaatca
      421 gctaatttct ctatattcta aacaaacaca taaatgggtg atgagctatg tttaaaagaa
      481 ctaaactaat cttaatagca acattactgc tatcaggatg ttcaactacc aataacgaat
      541 ccaacaatga aacaaaatca gtaccagaag aaatggaagc ttccaaatat gtaggacaag
      601 gctttcaacc acctgcagaa aaagatgcga ttgaatttgc gaagaagcat aaagataaaa
      661 tagctaaacg tggtgaacaa ttttttatgg ataacttcgg tctaaaagtt aaagctacca
      721 atgttgtagg tagtggcgac ggtgtagaag tattcgtgca ttgtgatgac cacgacatcg
      781 tatttaatgc gagtattcca tttgacaaat caattattga cagtaatagc tcattaagaa
      841 gtgaggacaa aggcgatgat atgagtactt tagttggtac agtgttgagt ggctttgaat
      901 atcgagcaca aaaagaaaag tatgacaatt tatataaatt tttcaaagat aatgaaaaga
      961 aatatcaata tacaggattt acaaaagagg caattaacaa gacacaaaat agtggatatg
     1021 aaaatgaata tttttatatt acgactatac cgtataattt aactgagtat agagactatt
     1081 ttgaaccatt attaaacaaa agtgacagtg aattttcaaa agaattgtca aatgttaaga
     1141 agcaattaaa agataagtct aaactttcgg taactactac tctatttagt aaaaaaaaga
     1201 actatactaa aaaaagtaac agtgaaaatg taataaaaat ggcagaagaa ataaaaaaag
     1261 aaaaagagat accaaacggt atagatctta gtataaaatt ttcggacaat aaaataaata
     1321 cagttaaacc aaattataac ggtaaaagca cttcagaata tggtgtgttt gactaagaat
     1381 aaaataaatg atgaatattt agcggagatt agtgtatatt ggctagatta agtattgaaa
     1441 gacaattcta ctattgttgt gaaggcaaat aagtagaagg ttttaagtat aacttctggc
     1501 gatttaaata ataatatata tggaagtgct gatacaaaac tttttaacct actagattct
     1561 tataatttgc tttccatttt atgacgattt tgacccaatt gagtgataga atcaaaaaaa
     1621 ccatcccaac aacagacaaa accacaaacc aatcaaacct acccaatcaa agtacgcaaa
     1681 tcaccaatca aaactaccca aacaatgctc gcaaatcacc aatgaaacat tctccaatta
     1741 cgtaactatg atctaattaa agcatagtta ttgaggtttt gtgatatata gtataaaatt
     1801 aatgagaatt aaatttaata atgtaaaatt catcttcggg gtcgggtgta attcccaacc
     1861 ggcagtaaat aaagcctgcg acctgctagt atgtatcata ttagtggctg atctagtgag
     1921 attctagagc cgacagtata gtctggatgg gagaagatgg aggttttttg ttgtgcaata
     1981 atcctcctat tcttacgaga tgaatggaag gagaaaattg aatatgcaac aaaataaacg
     2041 tcttatcaca ataagtatgt tgagcgcgat tgcgtttgta ttaactttta tcaagtttcc
     2101 tataccattt ttgccaccat acttaacttt agattttagt gatgtaccgt cactactagc
     2161 tacatttacg tttggaccag ttgccggtat catagttgca ctggttaaaa atttattgaa
     2221 ctacttattt agtatgggcg atccagttgg accatttgct aactttttag caggcgcaag
     2281 tttcttatta actgcttacg ccatctataa aaataaacgt tcaacaaaat ctttgattac
     2341 tggattaatc attgcaacaa tcgttatgac tattgtgttg agtatcttga actatttcgt
     2401 tctattacct ttatacggta tgatatttaa cttagctgat atcgcaaata atcttaaagt
     2461 aatcattgtt tcaggaatta taccattcaa tattattaaa ggtatcgtca tttctattgt
     2521 atttatttta ctatatagaa ggcttgcgaa tttcttgaaa agaatttaat caaattaaag
     2581 caaaataata tacacataat aataaaaagc aggtgactat caataaacga tagcttgcct
     2641 gctttttcta tgaaacattt gtctaaaaaa tcaattattc aaattttaaa gcgtccccat
     2701 caaatgattc gtctgcaatt ttaatagaat ctgtagggca tccatcaatt gcatcttcca
     2761 tatcttcata taattcctca ggtacttctg cggtaccttg gttatcgtca aggattacga
     2821 aagcaatacc ttcgtcgtcg taatcatata tatctggcgc tgctgcaccg catgcaccac
     2881 atgcaataca agtatccata tcaacgattg tatattttgc caatgtcttc gcctcctttg
     2941 ataaaaatgc taaaatagta atgtgactaa aattttagac agcatcattt ttattttcaa
     3001 attatccgtt ttacagagtg agggttaaac ttgcaacaca ttataaaaac agcattacaa
     3061 caaacattta actataaaac aaataaaagt atttacaata tcttagttgg taagaaatct
     3121 caccaaacct tttttgacgc ttgtagtcaa caacagttgt cattatatca cagtttacca
     3181 ctattaaaat atccgtcttt tgagctattt ttagaaaata taactgaatt taatgctgaa
     3241 atggaaatca tgttgcatcc tagatataca tttgaaagta tggggcaaac atttcaagca
     3301 attcaactat tagtgcaaac catgtctaat actatgcagc aagattttcg tttcgtacca
     3361 atctctcaaa ataataagat acaagaaact gtaaaaattg tctataacta tattaaagaa
     3421 aaccaattac aaattgactt tgaaaatgaa ctacataact tgtttaaagc aataacttta
     3481 aaagggccat gttacttaca ttattatttg caaggctatg atgaaccaat gtatacgaga
     3541 cagcaagtta gtttaataga aaaactatct caacagcaat tgtttgaatt cgaaatgaat
     3601 aatttagtga caatgatgtt tgaattagaa agtggagaat atactatttt atcaaaaata
     3661 ataatgaaac ctacattatt aaatcaaacc tatattactt atacaagatt gcttgaacaa
     3721 ttcacgatag aagatatagc ggctcaacaa caagttaaaa tcaatactat cgaagatcat
     3781 gtacttgaaa tcttaatcaa aggttatatg tctaattacg atgattatgt tgaacaagaa
     3841 gatcaactgc aatttttgaa tttttatcaa caacatcgtg gtgaacgatt aaaattttac
     3901 aaagaacaat ttgacacgtt atcatatttt caattaaaag tattaatcgt tggttttgaa
     3961 agaggtgatc tgaatgttgc atgatatttt acgaaacaaa tttggcttcg agaattttaa
     4021 accgggacaa caggaaatta tagaaagtat aatgtctcaa caacacactc taggtattct
     4081 tccaactgga agtggaaaga gtttgtgtta tcaaatacct acgtatttat caggtaagcc
     4141 gacattaatt atctcaccgt taatatcttt aatggatgac caagttatgc agttgaaaat
     4201 aaatggagaa aaacgtgtaa catgtattca ctctggtatg gatgaaattg agaaaaagca
     4261 taatattaaa tgtttacgac atagccgctt catctttcta agtccagaat ttctcctgca
     4321 gccgtcaaat tttaaattaa tatctatgat agactttggc atgattgttc tagatgaagc
     4381 acattgtcta tctgaatggg gatatgattt cagaccacat tatgctctaa ttggaaaagt
     4441 aacaaagcat tttaaagaag cggttgtctt agcattgaca gcaactgcac caccgcattt
     4501 gcaagatgat ttgacggaaa tgctagagat tcaattcaac gttattaaaa ctacaatgaa
     4561 tcgcccaaac ataagcttta agcatcttaa ttttcatgat gatgaagata aaattgagtg
     4621 gttgctgccg tttctacaac agtcgggacc aacgattatt tatgtctcat cgaaaaagat
     4681 gtgtctgaat ttagcgcaac ttatttatga ttcaggtttt cttactggca tttatcatgg
     4741 tgatatgaat tatcaagagc gacacacagt tcaacaacaa tttttaaata atgatattcc
     4801 gattatagtc gcaacgagtg cttttggtat gggaattaat aaaaaagata ttcgcacaat
     4861 cattcacttt catctttcaa caagtccttc taactacatt caagaaattg gacgtgcggg
     4921 tcgcgatggt gaactaagtc aggcaattag tttatttcaa ccggacgata aatatatttt
     4981 agaaacgtta ttatttgcag atatgataac agaagaagat gtacaaaatt tcgaaatagg
     5041 agaattttta gcacccgata aacaagacgt tttgacaacg ttgcattcat tctatagtat
     5101 cggcgccttg aaacaggtat ttaagaaatc atttaaacga aagcaattag gattctttcg
     5161 tatgattggc tattgcaaat tggatcaatg tagacggaag tatttattag aatttttcgg
     5221 tgaatatcca ccggcacaag atcgatgttg tgacaatgat tctaatataa ctgatatcgc
     5281 aattttaaat aagaagaagg taattagaag tattggattt gatgaaaagt tgcaaaattt
     5341 atttctcaga tagtattact ttactaaaag aaaattgaca agctataatt agtgtataca
     5401 caattgaaaa atgattgaaa taattttgaa aaatatacat aaacatatgt catgtgggta
     5461 tattttatgt aaaatcattg taatagaata gaaaggaaga tggctatgtc taataatttt
     5521 aaagatgact ttgaaaaaaa tcgtcaatcg atagacacaa attcacatca agaccatacg
     5581 gaagatgttg aaaaagacca atcagaatta gaacatcagg atacaataga gaatacggag
     5641 caacagtttc cgccaagaaa tgcccaaaga agaaaaagac gccgtgattt agcaacgaat
     5701 cataataaac aagttcacaa tgaatcacaa acatctgaag acaatgttca aaatgaggct
     5761 ggcacaatag atgatcgtca agtcgaatca tcacacagta ctgaaagtca agaacctagc
     5821 catcaagaca gtacacctca acatgaagag ggatattata ataagaatgc ttttgcaatg
     5881 gataaatcac atccagaacc aatcgaagac aatgataaac acgagactat taaagaagca
     5941 gaaaataaca ctgagcattc aacagtttct gataagagtg aagctgaaca atctcagcaa
     6001 cctaaaccat attttgcaac aggtgctaac caagcaaata catcaaaaga taaacatgat
     6061 gatgtaactg ttaagcaaga caaagatgaa tctaaagatc atcatagtgg taaaaaaggc
     6121 gcagcaattg gtgctggaac agcgggtgtt gcaggtgcag ctggtgcaat gggtgtttct
     6181 aaagctaaga aacattcaaa tgacgctcaa aacaaaagta attctggcaa ggtgaataac
     6241 tcgactgagg ataaagcgtc tgaagacaag tcaaaagaac atcataatgg taaaaaaggt
     6301 gcagcaatcg gtgctggaac agcaggtttg gctggaggcg cagcaagtaa tagtgcttct
     6361 gccgcttcaa aaccacatgc ctctaataat gcaagtcaaa acaatgatga acatgaccat
     6421 catgacagag ataaagaacg taaaaaaggt ggcatggcca aagtattgtt accattaatt
     6481 gcagctgtac taattatcgg tgcattagcg atatttggag gcatggcatt aaacaatcat
     6541 aataatggta caaaagaaaa taaaatcgcg aatacaaata aaaataatgc tgatgaaagt
     6601 aaagataaag acacatctaa agacgcttct aaagataaat caaaatctac agacagtgat
     6661 aaatcaaaag atgatcaaga caaagcgact aaagatgaat ctgataatga tcaaaacaac
     6721 gctaatcaag cgaacaatca agcacaaaat aatcaaaatc aacaacaagc taatcaaaat
     6781 caacaacagc aacaacaacg tcaaggtggt ggccaaagac atacagtgaa tggtcaagaa
     6841 aacttatacc gtatcgcaat tcaatactac ggttcaggtt caccggaaaa tgttgaaaaa
     6901 attagacgtg ccaatggttt aagtggtaac aatattagaa acggtcaaca aatcgttatt
     6961 ccataatata actatataaa ttgtaactga actgctatat acgaacgtgc atattataaa
     7021 gtttctctaa atattattga aaacttaaat tgcgcttata agtatgtagc agttttttta
     7081 tttaataagt atggacatga atattggcat atcaattaaa ttttttacat agtcatcaaa
     7141 aagataaaac accacaatca acaaatttaa cgaggaagaa taaaaaataa atcaacatat
     7201 taaattgtag tgttattcaa ctccgtagct aacaattctc tatgcatatt aaacaaattg
     7261 tcgaaaatat atcataaatc ttcaagcaca gacttagcgc atcaatcact gaactgttat
     7321 aatagtttgg gattaaagga ggccggaaca atgcaaaaag ttgaaagtat cataattggt
     7381 ggagggccat gcggattaag tgcggctatt gaacaaaaaa gaaaaggtat tgatacctta
     7441 attattgaaa agggtaatgt cgttgaatca atctacaatt atcctactca ccaaacattt
     7501 ttctcatcaa gtgataaatt aagtattggg gacgtaccgt ttattgttga agaaagtaaa
     7561 ccaagacgta atcaagcgct agtttattac cgagaagttg taaaacatca tcaattaaaa
     7621 gtaaatgcat ttgaagaagt attaactgtt aaaaaaatga ataataaatt tactattact
     7681 acgacgaaag atgtttatga atgtcgattt ttaacaatcg cgacaggcta ttatggtcag
     7741 cataatacat tagaagttga aggtgcggat ttacctaaag tgttccatta ttttaaagag
     7801 gcacatccgt attttgatca agatgttgtt attatcggtg gtaagaattc ggctatcgat
     7861 gctgctttgg agttggaaaa agctggtgct aacgtgacgg ttctatatcg tggtggagat
     7921 tattcgcctt caattaaacc gtggatactt ccaaatttca cagcattagt aaatcatgaa
     7981 aaaattgaca tggaatttaa tgctaatgtt acccaaataa ctgaagatac tgtgacttat
     8041 gaagtaaatg gtgaaagtaa aacgatacac aatgattatg tatttgcgat gattggttat
     8101 catcccgatt atgaattttt aaaatctgta ggcattcaaa ttaatacaaa tgaatttgga
     8161 acagcgccta tgtataataa agaaacatac gaaacaaata tcgaaaattg ctacattgca
     8221 ggtgtgattg ctgcagggaa cgatgcgaat accattttta ttgaaaatgg taaattccac
     8281 ggtggtatta ttgctcaaag catgctagct aagaaacaaa cgccgttaga atcataaaaa
     8341 taaaggtcta tgtactaaat aacttagttt tacaacgact gacattcatg atatgtcaaa
     8401 tgaggttgat gactaattat tgtacataga cctttttatg ttacgtaatc attataattc
     8461 aaaatatgat ttgatttcag ctttatctaa attattgctt aacgcgacta ataattttaa
     8521 tcttgctttt ggaccattca aaccgttaga aaaaataaaa ccttgttgtg cgagttggta
     8581 accaccacca tcgtatgcgt aagttggact cacaatacca ttaaaggaac gtgaaactag
     8641 cacaataggt atatttaaag atactaattg ttgaatgcct tctaatgcgc ttggaggcat
     8701 gttgccttgc cctaacgctt caataaccat accatcgata ccttcacgac tataaaaact
     8761 aaaaatgtca cctggcatac ccatatatgc ttttactaat ggtacattta gtttctcatt
     8821 gacattttcc aatgcttgtt ggcgatatgg catatgatgg aattgcacac gatcctttgt
     8881 caatacacct agcggaccat gatttggact ttgaaatgtg tttgtattag acgtatgtgt
     8941 tttggtaaca ttacgcgccg tatgtatttc atcattaaat acaaccatca cgcctttatg
     9001 tcgggccttt tcatcagagg caacgcgaat agcggaaata taattatata atccgtcaga
     9061 accaatttca ttagacgagc gcattgcgcc agtaataaca acaggttgct cgatacctaa
     9121 tattaaatca agtaaaaagg cagtttcttc taacgtatcg gtaccatgcg tgataacgaa
     9181 accatcataa tatttatttg ttactgcttc taaaataata tcctttaatt gtttaacatg
     9241 ttggattgtc atatgaggtg atggtacatt aaaaggattt aattcatcta tttgtgcata
     9301 ttgatttatg acatcttgat gcagtgaaat agggttaata tcatttgtta ctactttatt
     9361 agattggtct tgtgacatac taatggtgcc accagtatga ataacaagta gatgtttcat
     9421 atatttcctc ctatatttca tttacctaat tatgataaaa tattattcat aaaacgacaa
     9481 ggaagggaaa tgacgcatga aagccattaa tattgcatta gatggtccag ctgctgccgg
     9541 aaaaagtaca attgcaaaac gtgtagccag tgaactatca atgatttatg ttgatacagg
     9601 agcaatgtat cgtgcattaa catacaaata tttaaaatta aacaaaactg aggactttgc
     9661 aaaactagtt gaccaaacaa cattagattt aacttataaa gcagataaag gtcaatgtgt
     9721 cattttagat aacgaagatg taacagactt tttaagaaat aatgatgtga cgcaacatgt
     9781 ttcatacgtt gcatctaaag agccagtacg ttcattcgcc gttaaaaaac aaaaagagtt
     9841 agctgcagaa aaaggtatcg taatggatgg tcgcgatatc ggaactgtag tgctaccaga
     9901 tgcagattta aaagtatata tgattgcatc agttgaagag cgagcagaaa gaagatataa
     9961 agataatcaa ttaagaggaa tcgaatcaaa ttttgaagat ttaaaacgtg atattgaagc
    10021 acgtgatcaa tatgacatga accgtgaaat atcaccatta agaaaagcag atgatgcagt
    10081 gacattagat acgacaggca agacgattga agaagttact gacgaaattt tagcgatggt
    10141 gagtcaaatt aaataatcta gtaagtcata aatatctatt tgtaatgtgc aacgcttaat
    10201 gcatatacaa attgaatgtg ctgataatga tttactcaac ttaaaaggtg atttttattc
    10261 aacgatgaat gaaagttgcc tttttacttt tggtaaaagt taatgcgtca gtcaagtgtg
    10321 tatgtttttc aaaaagttaa cagaaataat aaaggtaaat tattagaatt ccaaaaataa
    10381 ttcattaaat tcataaagca ttttacaaat ggtaagaaaa tgagtgttac aaatctaaga
    10441 attgcaaaag aagctgattt agtcacaaaa aatgtcctat gtaataattc gagaaagatg
    10501 cactatatac ggtcttctta ctattcaaat gtaaaagttg cttatttgcg tggctttttg
    10561 ttttataaaa gtataaaatt ttactataat atatcttgta gagaacaatg aaatgacaga
    10621 cttataaaac aaaatagtga gctatgctaa tttttataca aaaaatatgt ataaacatag
    10681 cggactttat tacttgtttg aatatatggt tgtaataaat atatgtccaa ctaatacaca
    10741 gaatgagatt aagtgacata tttatggata cgactaattt actaaatgta ataaaggtag
    10801 aatttcttga caattctgtc agtttataag atgttataaa tatgtagtgt ataaggaggc
    10861 aaacaagatg actgaagaat tcaatgaatc aatgattaac gatattaaag aaggtgacaa
    10921 agtcactggc gaggtacaac aagttgaaga caagcaagtt gttgttcata tcaacggtgg
    10981 taaatttaat gggattattc ctattagtca actatctacg catcatattg atagcccaag
    11041 tgaagttgta aaagagggcg acgaagttga agcatatgtc actaaagttg agtttgatga
    11101 agaaaatgaa actggagctt acatcttatc tagaagacaa cttgaaactg agaagtctta
    11161 tagttattta caagaaaaat tagataataa tgaaatcatc gaagcgaaag taacagaagt
    11221 agttaaaggt ggtttggttg ttgatgtagg acaaagaggt tttgttccgg cttcactaat
    11281 ttcaacagac ttcattgagg atttctctgt gtttgatgga caaacaattc gtattaaagt
    11341 tgaagaattg gatcctgaaa ataatagagt cattttaagc cgtaaagcag ttgaacaaga
    11401 agaaaacgat gctaaaaaag atcaattatt acaatcttta aatgaaggcg atgttattga
    11461 tggtaaagta gcgcgtttaa ctcaatttgg tgcatttata gacattggcg gtgtcgatgg
    11521 tttagtgcat gtatctgaac tttctcacga acatgttcaa acaccagaag aagtagtttc
    11581 aattggtcaa gatgttaaag ttaaaattaa atctattgat agagatacag aacgtatttc
    11641 attatcaatc aaagatacgt taccaacacc tttcgaaaat attaaaggtc aattccacga
    11701 aaatgatgac attgaaggtg tcgtagtaag attggcaaac tttggtgcat ttgttgaaat
    11761 tgcaccaggt gtacaagggc ttgtacatat ttctgaaatt gcacacaaac acattggtac
    11821 gccaggtgaa gtattagaac ctggtcaaca agtaaatgtt aaaatattag gtattgatga
    11881 agagaatgaa agagtatcac tatctattaa agcaacatta ccaaacgaag atgttgttga
    11941 aagtgatcct tctacgacta aggcgtactt agaaagcgaa gaagaagata atccaacaat
    12001 tggcgatatg attggtgata aacttaaaaa tcttaaacta taatttaata tttaatagtc
    12061 aactccacat gtttatgatt gcatgtggag tatttttatg taacaaaata tatccggaat
    12121 gataacgtgg acaaatttaa ctaagtgttt tttaagataa agttttaagt gctgattttt
    12181 atcattacag taataaactc attttgaata cacagtctca tgtgatatta ttaaaaagat
    12241 ataagaaaga gaggaagtta gcttatgact aaacctatag tagctattgt aggtaggcct
    12301 aatgtaggta aatctacaat ttttaataga atagttggag aacgtgtttc gattgttgaa
    12361 gatacgccag gtgtaacacg agatcgtatt tattcttcag gtgagtggtt aacacatgat
    12421 ttcaatatta ttgatacagg tggtattgaa attggtgatg caccattcca aacacaaatt
    12481 agagcgcagg cagaaatcgc catagatgaa gcggatgtta ttatttttat ggttaacgtg
    12541 cgtgaaggat tgacacaaag cgatgaaatg gtcgctcaaa ttttatacaa atctaaaaaa
    12601 ccggtcgtat tagcggttaa caaagtagat aatatggaaa tgcgtacaga cgtgtatgat
    12661 ttctattcat taggatttgg tgaaccgtat ccgatatcag ggtcacatgg tttaggtctt
    12721 ggtgacttgt tagatgcagt tgtttctcat tttggtgaag aggaagaaga tccttatgat
    12781 gaagatacaa ttcgactatc cattattgga cgaccaaacg taggtaaatc aagtttagta
    12841 aatgctattt taggtgaaga tcgcgttatc gtttctaatg ttgcagggac aacgagagac
    12901 gctattgata cagagtatag ttatgatgga caagattatg ttttaatcga tactgctggt
    12961 atgcgtaaaa aaggaaaagt atatgaatca actgagaaat attcagtatt aagagcttta
    13021 aaagcgattg aacgttcaaa tgttgtttta gtggtcattg atgcagaaca aggcattatt
    13081 gaacaagata aacgtgttgc aggatatgca catgaacaag gtaaagcagt cgtgattgtc
    13141 gtaaataaat gggatactgt ggaaaaagat agtaaaacga tgaagaaatt tgaagatgaa
    13201 gtacgtaaag aattccaatt tttagattat gcacaaattg cttttgtgtc tgctaaagaa
    13261 cgcacaagat tacgtacatt attcccttac attaatgaag caagtgaaaa ccataaaaaa
    13321 cgtgttcaaa gttcaacttt aaatgaagtc gttactgatg caatttccat gaaccctaca
    13381 ccaacagaca aaggtagacg tttgaatgtc ttttatgcaa cacaagttgc tatagaacca
    13441 ccgacatttg ttgtatttgt taatgatgta gaattaatgc atttttctta taaacgctat
    13501 ttagagaatc aaatccgtgc cgcttttggt tttgaaggta caccaattca tattatagct
    13561 cgaaagagaa attaacgatt gggggataac aatgactaaa attaccgttt ttggtatggg
    13621 aagttttggg acagcccttg ccaatgttct tgcagaaaat ggacatgatg ttttgatgtg
    13681 gggtaaaaat caagaagctg ttgatgaatt aaatacatgt catacaaata aaaagtattt
    13741 aaaatacgcg aaattagatg ttaacatcat cgctacttca gatatgacca aagcaattca
    13801 atatgcagat atttacttaa tggctttacc tactaaagca atgcgagaag ttgctactca
    13861 aattaatgat aagctgacat ctaaaaagac ttttatacat gttgctaaag gtattgaaaa
    13921 tgggacattt aaacgtgtgt cagaaatgat tgaagattct atttcacctg aatataatgc
    13981 aggtattggc gtgttgtcag ggccaagtca tgcggaagaa gttgtagtca agcaaccaac
    14041 tacagttgct gcatcatcaa aagataaaaa tgtaagtaaa ttaacgcaag atttatttat
    14101 gaatgattat ttgcgtgtgt acacgaatga tgacttggtt ggtgttgaac ttggtggtgc
    14161 attgaaaaat atcatcgcag tagcaagtgg tatcgtagct ggaattggct acggtgataa
    14221 tgcaaaagct gcattaatga ctcgtggctt agcggaaatt agcagattag gtgaaaagtt
    14281 aggtgccgat cctatgacat ttctaggttt aggtggtatc ggtgacttaa tcgttacttg
    14341 cacatcaaca cattctcgaa atttcacatt aggatataag cttggacaag gtgaatcgat
    14401 ggatcaagca ttatctgaaa tgaatatggt tgttgaaggt atttatacaa ctaaatccgt
    14461 ttatcattta gctaaagaaa aaaatgtgga tatgccaatt acaaatgcat tatatagagt
    14521 attatttgaa aatatctcag taaaagaatg cgtaaaagat ttaatggagc gcgataaaaa
    14581 atctgaataa aatgtgtatt attacacata tacagctaat aattacgtaa aatgaataga
    14641 aatggcttaa attcaacgtt tttgccacat aaatgattgc gatttatgta atgcttgtgt
    14701 taaagtttta ccataatgat ataaacatat cattatcaaa cctcaggagg tgaatgtcta
    14761 atgaacaaaa cagatttaat caatgcagtt gcagagcaag ctgatttaac taaaaaagaa
    14821 gctggttcag cagtagatgc tgtattcgaa tcaatccaaa actcacttgc taaaggtgaa
    14881 aaagtacaat taattggttt cggtaacttt gaggtacgtg aacgtgctgc acgtaaaggt
    14941 cgtaaccctc aaactggtaa agaaattgat atcccagcaa gtaaagttcc agcattcaaa
    15001 gctggtaaag cattaaaaga tgctgtaaaa taattttact taaaaagccc tgaataaggg
    15061 ctttttattt tgcttttaat acttacaacc tgtacataaa ttgtaatgtt cttctaagtt
    15121 tttaatcttt ggaacattca ttgtttaagc gttcaaaata ttacatgaca taatagttt
//
LOCUS       DATTQF010000054        15149 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.054, whole genome shotgun sequence.
ACCESSION   DATTQF010000054 DATTQF010000000
VERSION     DATTQF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.054"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            366..806
                     /locus_tag="VB241_001405"
     CDS             366..806
                     /locus_tag="VB241_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEP1244703.1"
                     /translation="MNTKYFLAAGAIITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1149..1622
                     /locus_tag="VB241_001406"
     CDS             1149..1622
                     /locus_tag="VB241_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEP1244704.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1597..2283
                     /locus_tag="VB241_001407"
     CDS             1597..2283
                     /locus_tag="VB241_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1244705.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2283..3338
                     /gene="saeS"
                     /locus_tag="VB241_001408"
     CDS             2283..3338
                     /gene="saeS"
                     /locus_tag="VB241_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HEP1244706.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITT"
     gene            complement(3410..4393)
                     /gene="csbB"
                     /locus_tag="VB241_001409"
     CDS             complement(3410..4393)
                     /gene="csbB"
                     /locus_tag="VB241_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HEP1244707.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVARQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(4525..5364)
                     /locus_tag="VB241_001410"
     CDS             complement(4525..5364)
                     /locus_tag="VB241_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1244708.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5578..6927)
                     /locus_tag="VB241_001411"
     CDS             complement(5578..6927)
                     /locus_tag="VB241_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP1244709.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLDTIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEPRPTIGQSDEDEKSE"
     gene            complement(7148..8329)
                     /gene="nagA"
                     /locus_tag="VB241_001412"
     CDS             complement(7148..8329)
                     /gene="nagA"
                     /locus_tag="VB241_001412"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP1244710.1"
                     /translation="MSELIIYNGKVYTEDDKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALINIAKYEVEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAAE
                     RGAKHVTHLYNAATSFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLTNGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8632..10590)
                     /locus_tag="VB241_001413"
     CDS             complement(8632..10590)
                     /locus_tag="VB241_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1244711.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDIASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKVGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIKNATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFDPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKVMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALVVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10596..11516)
                     /gene="pfkB"
                     /locus_tag="VB241_001414"
     CDS             complement(10596..11516)
                     /gene="pfkB"
                     /locus_tag="VB241_001414"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP1244712.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLSIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTICVLDGE"
     gene            complement(11513..12277)
                     /locus_tag="VB241_001415"
     CDS             complement(11513..12277)
                     /locus_tag="VB241_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP1244713.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYKQKYHFIGGTL"
     gene            complement(12527..13009)
                     /gene="ybaK"
                     /locus_tag="VB241_001416"
     CDS             complement(12527..13009)
                     /gene="ybaK"
                     /locus_tag="VB241_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEP1244714.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENAKHEHFVFVIPVSETLDMKKAAAMVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13183..13635)
                     /locus_tag="VB241_001417"
     CDS             complement(13183..13635)
                     /locus_tag="VB241_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244715.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(13932..15098)
                     /gene="norA"
                     /locus_tag="VB241_001418"
     CDS             complement(13932..15098)
                     /gene="norA"
                     /locus_tag="VB241_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HEP1244716.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADVSPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSVVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPAILTLVLAFGL
                     SAFETLYSLYTSYKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     IYSVIVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGDRQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKQQDL"
BASE COUNT         5010 a         2830 c         2083 g         5226 t
ORIGIN      
        1 ttttaacacc atattggtac ttgtatttaa tcgtctatct tattttcttg ttgtttttat
       61 tgtggcaaaa ggtttataaa ttttaatacc aaaactaaaa aacacttctg atattcttag
      121 ttcgctatat cagaagtgtt ttatagtgat aactagttta gataaatatt ttctcactta
      181 aaaaaccgcc ctcctcttat tttgactcct atttatttaa atcagacaat tattttcatt
      241 ttcaaattat tctttcttca atattagtta agcgatattt aaacgaagtt aagaattagt
      301 taatggcata ttatttgcct tcattttaaa cttaacttat caaattgaag aaatgaggag
      361 ttagcatgaa tacaaaatat tttttagcag ctggtgctat tatcacaaca ttagctttag
      421 gcgcttgtgg taattctaat tcacaagatc aaggtaacaa aactgaacaa aaaacaaagt
      481 cagaagatag caatgttaaa actgataaaa caaaacacct aacaggtaca ttcagttcta
      541 aaaacggtga aactgttgaa ggtaaagctg agattaaaaa tggtaaatta atgcttacta
      601 actacaaatc atcaaaaggt ccagatttat acgtctacct aacaaaaaat ggcgacatta
      661 aaaacggtaa agaaatcgca atggttgact acgataaaga aaaacaaaca tttgatctta
      721 aaaatgtaga tttaagcaaa tatgatgaag taacaatcta ttgtaaaaaa gctcacgtca
      781 tcttcggcgg cgctaaatta aaataatcaa tttctgagtt aaacttttat ttacaacata
      841 ctattactat acattacaaa ttttaaaaat atacattaca ctcattactc aatggaaagc
      901 gtatgatttc acagcccccc ctagcttgta gaaatcatac tttccttttt tcaatatata
      961 tacaactatt aaatcccata agattgtaga gcacataagt aaattttttt agagcttgag
     1021 gtttgtttag cttaagcaac ccatgagctc aaacacttcc tgttcacata acactacaaa
     1081 tcacattatg ttgctaaatc ttatgtttat ataaattaca cacaataaat agaaagaatg
     1141 tgaacatcat gaataaatta ttgctactcg ttacatttat cattcgtgtg ggttcaggta
     1201 ttgttatgtt aatgcaaggt tacgaaaaat taacgggcgg atttacgctg aaaggtttag
     1261 taccagtcat cgctaacaat actgattcac cagagtggta taagtggttt ttcgcaaata
     1321 tagttgcaca tacgacgtca ttatttgata ttgttgtccc actcggagag attgcaattg
     1381 gattaggttt aatttttgga gtttttgcat atgctgctag tttctttgga gcctttgtta
     1441 tgataaatta tatcttagca gatatgatat ttacgtatcc tcttcaatta actttcttta
     1501 tccttttatt aatgagtcac tcattgttaa aacagatttc acttaaagaa atcattaatt
     1561 actttagagg tcgtaagaac agaggtgaaa aaatagatga cccacttact gatcgtggat
     1621 gatgaacaag acattgtaga catttgtcaa acctattttg aatatgaagg ttacaaagta
     1681 acaacgacaa ctagcggtaa agaagcaatt tctttactat caaatgatat tgatatcatg
     1741 gtacttgata tcatgatgcc agaagttaat ggttacgaca ttgtcaaaga aatgaaaagg
     1801 caaaaattag atatcccctt tatctattta actgccaaaa cacaagaaca tgataccatt
     1861 tacgccttaa ctttaggtgc agatgactat gtcaaaaaac catttagtcc aagggaactc
     1921 gttttacgta ttaataattt acttacaaga atgaagaaat accatcatca accagttgaa
     1981 caactgtcat ttgatgaatt aacacttatt aacttaagta aagttgtgac tgtaaatggt
     2041 catgaagtcc ctatgcgtat taaggaattt gagttactgt ggtatttagc ttctagagaa
     2101 aatgaagtta tttctaaatc agaattactt gaaaaagttt ggggatatga ctattacgaa
     2161 gatgctaata ccgtgaatgt ccatatacac cgtattagag aaaaattaga aaaagagagc
     2221 tttacaacat ataccatcac aactgtatgg ggattaggat ataaatttga aaggagccga
     2281 taatggtgtt atcaattaga agtcaaatca ttattggcgt cgtatcgagt atactattaa
     2341 cttcaactat tttagcaatt gcatatattt taatgtggtt taacggccat atgacactaa
     2401 ctttgacctt aacgacaata attacaagct gtttaacctt attaatatgt agtattttta
     2461 ttaatccact tatacaaaaa attaagcagt ttaatataaa aactaagcaa tttgctaacg
     2521 gaaattacgc aagcaatgat aaaacgttta attcaccaaa agaaatttat gaattaaatc
     2581 aatcttttaa taaaatggct tctgaaatta cgcaacaaat gaatcaaatt aaatccgaac
     2641 aacaagaaaa aacagaactg attcaaaact tagcccatga tttaaaaaca cctttagcaa
     2701 gcattatttc atattctgaa ggactacgtg atggtataat cacaaaggat catgagatta
     2761 aagagtcata cgacatatta attaaacaag caaacagatt atcaacatta tttgatgata
     2821 tgactcatat tatcacttta aatacaggta aaacatatcc cccagaatta atacaactag
     2881 accaattact tgtatcaata ttgcaaccat atgagcaacg catcaaacat gaaaaccgca
     2941 cattagaagt gaatttctgt agcgaaattg atgcatttta tcaatatcga acgccacttg
     3001 agcgtatttt aacaaactta cttgataatg cgctaaaatt ttcaaatgtt ggtagtcgca
     3061 ttgatattaa tattagtgaa aacaaagatc aagatactat cgacattgct attagcgatg
     3121 aaggtattgg cattatacca gaactacaag aacgtatatt cgaacgtaca ttcagagtag
     3181 aaaactctcg taatacaaaa acgggtggtt ctggattagg cttatatata gctaatgaac
     3241 tcgcgcaaca aaataacgca aaaatcagtg taagcagtga tatagatgta ggaactacga
     3301 tgactgtaac attacacaaa ttagacatta cgacataatc cgatttattt ataaaataaa
     3361 ctgcaaagac taaaaagaag ctcccattaa tgagggcttc tttttttgtt tatttagaat
     3421 aaactttatg ggtatccttc tcatcatttt caatacttga aagatttgta gcttgaataa
     3481 tgtatttagg acgtgcctta acttcataat atatcctgcc aatatattca cctacaacac
     3541 caattgaaat taactgtatg ccgcctaata ataaaatagc tgcaatcgtt gaaaaatatc
     3601 ctgggatatt aacaccagaa atcataatat tgatgaataa atagataata tataacacgc
     3661 ttattgaaaa aataaataag ccaagatata tcatcattct caaaggttta ctattaaagg
     3721 aaatcaatcc atcaatacca taattaaata actttttaaa ggaccattta gattctcctt
     3781 tttgtctcgc aacattttga tacgtaaaca ctttagtatt atatcctatc cattcaaata
     3841 acccttttga aaatcgatta tattcttcaa gtgatgcaat ggatttaaca gctctttggc
     3901 ttaaaagtct aaaatcacca acaccatcat caaattgtac ttcttctaca aagcaattaa
     3961 ctaacttata gtacaaatgg cttaatgttt tgcgactaaa attttcacca cttctatcac
     4021 gctttgcaat cacttgatca tagccttcca taaaaccttc aaccattttc ggaatatatt
     4081 caggtggatg ttgcaaatca caatctatca tgatgactgc atcaaattca gtactatgct
     4141 ggtaacctgc aatcatagct gcttcttttc caaaatttct actaaaagaa ataaagctga
     4201 catgtctatc tattgtggca agatgttgca tttcatctat agtggtatcc gtactaccat
     4261 catctatgaa aagcatatta taatcatagc ctttcacact actatcttgt gaaagtattt
     4321 cagttaattg ttgatgtgtt tgtgtaatga cttccccttc attaaaacaa ggaatgacga
     4381 ctctaatttt catctatgtc catccttttt atttaaaaga ttttgaatgt acctgagtat
     4441 tgttttatca aatagtaaaa accaactcat ttattttaat gtacacatta aaaggcattc
     4501 ataaacgtaa aatttaacat ccagttattt taaatcccca attttcacat catctggatt
     4561 tttaccttgt cttgcatttc tatttaaagc atcaatttca gctacttctg ttaattctaa
     4621 attaaaatct aaaatatcta tattttcttg aatgcgttta ggtgtctgag attttggaat
     4681 aataattcta ttgtgtgcta aatgccaacg caatacaact tgtgctggtg ttttatgata
     4741 tttttcagca attttaacaa tgacagggtc gtctagtagt cctctatttc tcatcaaagg
     4801 catccatgca gtcactttaa tatcgtgacg atcacaaaat tcttgtaatt cttgttggtt
     4861 aaaatatgga tgtacctcaa tttgattcac cattggtttg atacttgatt gagccattaa
     4921 tttttctaga tgatgaacat taaaattaca tacacctatt gcttttacct taccttgctc
     4981 gtaaagttct tccatagctt tatatgtttc taaaaataga ccatctgctt cacaaggcca
     5041 atgtattaga aataaatcaa gataatcagt ttgtaaattt tcaatcgatt tgttgaaata
     5101 ttcgaatgtt ttctcataac cttgatagtc attccataac ttcgttgtta taaacaaatc
     5161 ttctctatcg acgccattat cctttaatgc tcgtcctagt gaagcctcat tatcataaaa
     5221 gtatgctgta tcaaacgctc tatagcctgc gtcaattgca gcatttacaa ctttagtcat
     5281 atcttcgtca gaaattttat aaacacctaa cccaactgaa ggcatcgggt atccattatt
     5341 taatatttgt atctcattca acatctttat atctctccaa tctatgtatc ttttatatct
     5401 ttacattacc ctaaattttt caacaaactc aattaatacg aattatcgct ttcaataaaa
     5461 aatattcatt aaagatattg agttccaata ctattttcac ttttcattaa ttttagtcaa
     5521 aaaaataacc aaccaaaaat gaattaaatc attcttagtt ggtttatata ttattatcta
     5581 ttctgatttt tcatcttcat cagactgtcc gatagtaggt ctcggttcat taaattcata
     5641 gtttaacatt acccaaataa tttggtggtt atcgatttct gaaacaaccc agcgatcata
     5701 cgttgtatcc acgtaatcat ctttttgtaa gtttgtattg cgagattgta accatccacc
     5761 tatcgtatca atatcctcag agtcatcaaa ttctataccg aactcttcag ttaaatcatc
     5821 caatagtact ctaccattta cttggaatgt cttattatca attttaacga tatcattcac
     5881 ttcatcatca tcaaattcat cacgaatttc cccaacgatt tcttctaaaa tatcttccat
     5941 cgttaaaata cctgccgttc caccatattc atctataata agactcatat gtacatgctc
     6001 acgttgcatt ctaattaatg catcactgat acgtgttgtc tctgaaatca ttggcaactc
     6061 gtgtatatag tttgctattt taatcgtttt tccagaagca tattcagtta aaaattcttt
     6121 aacgttgata aatcctttaa tgtggtcttt atcaccatca tcagtaattg gataacgtgt
     6181 aaattgatgt tcttttattg tatcaagtaa ttcgtctaca ttaaaaggtt catttagtgt
     6241 aatcatttgc gttctaggta ccattatatc ttttgcatgt ctttcatcga atgaaaagat
     6301 attttgcata tatgccaatt cagtttggtt gatttctcca ccattataac tattgttaat
     6361 aataattttg atttcttctt ctgacattgc atcagtttgg gcatcaggat ttacaccaaa
     6421 cattctaata ataacacgtg cagaaccatt catcagccaa atcaatggtt tcataatgtt
     6481 accgaaatag aacaatggtc ttgcatatac taaagcaagc ttttcagtat gttgaatcgc
     6541 tatagattta ggcgctaatt caccaagtac tacatgcaaa tacgtaacga ttataaatga
     6601 tactgcaaac gaaatcgtcg tcgttaatgc agttggtaaa ttgattgctt caaatatcgg
     6661 gtgtaatagc ttttcaaacg ttggttcacc aagccaacct aaccctaaag atgttacagt
     6721 tatacctaac tgacaagcag aaagataata atctagatta gcaatcatct tttttactat
     6781 tttagcaggt ttatttcctt catctgctag ctgttcaatt cttgttgctc taatttttac
     6841 taatgcaaat tctgaaccaa caaatactgt ggttaatgca attaatagaa taaatataat
     6901 caaactaatt atggtcgaag tttccaatta attccctatt tctagggatt cacctccatg
     6961 ttatatgtgt cactcatggg taacacgcat tcaaatttat cactatgact taaattacaa
     7021 ctacatgtta tcgccttccc atttagacac ccccagaaaa aaatgttatt gcctctattt
     7081 tatcataata taaagtgctt atgttaacag attaaatcta ttgcatacat ttaattatga
     7141 ttatctatta gctaaatgtg tgaaccttac cttgttttat tgtagatttt acattcatgt
     7201 catcatctag aataacaaga tctgcatcct tatttacttt aatactacct tttctatcat
     7261 cgatacctaa tgcaatggct tgatttaaac ttgttacacg ccataaatga tctaatgtat
     7321 cacctgtaaa tgatattaag ttacgtaacc catgattcat ttttaaaata ctaccagcaa
     7381 gtgcaccatt tgtaagacgt gcttgttgcg attgaacagt tactttttga ccacccaaat
     7441 catattctcc ttcaggcata ccttttgcac gcattgcatc ggtaattaaa taaaaacgtt
     7501 cattaccttt catacggtaa gcaattgcaa ccgatgccgg atgagaatgt gtgccatcaa
     7561 caatcatttc ggtatgtaaa gcatcattca accatgctgc tccaaaaaca cctggttctc
     7621 tatgttggaa tgacgtcgct gcattatata aatgcgtgac atgtttagct cctcgctcag
     7681 cagcttcaac tgcttcttcg tatgttgcca ctgtatgacc aattgaaaaa ataatgtcat
     7741 ctttatacgt ttcaagcgct tcttttgcac cttcaacttc aggtgcaaac gtcataatct
     7801 ttattagtcc gttagcagtc tcttgaaaat gtttaatttt atcgataaat gggcgtacaa
     7861 cgtattgcgg atgttgagca ccaactttat tttcagatat aaatggccct tccaaatgta
     7921 tacctacaat ttccgctgca ttgtgaacat cttgctccac ttcatattta gcaatattta
     7981 taagtgcttt atctatttta tcagtcgatt gcgtcattgt agtggccaag tacgatgtcg
     8041 tcccttcaga caacaaattt tcggatagat attttaagcc atcgtatgac ccatccattg
     8101 catcctgtcc ataaccacca tgaatatgta tatcgataaa acctggtaat acatgatgac
     8161 cttttgcatc aatcacttga attttatttg tcgtatcatt atcaatcgct gctttatcat
     8221 ccacttctcc aattgcaaca atctgtccat ctttcacatg aatgtaacca ttatcgattt
     8281 tgtcatcttc agtataaact ttgccgttat atataattaa ttctgacacg tataaaccct
     8341 tctttattta taaattttca ctttaagaca ctcatatttt actatatctg cttcgtttgg
     8401 cataactttt tgcaaaaact ttaaaaacta tatttatttt tgtaatgact acattcacat
     8461 actaaaaatt aaaaacacct tcgcatccat tcataaaaac tgaattaatg gaaggtgttt
     8521 taatagtttt atataaacta ccgttaacac gatatattaa attaacatac attcaatcga
     8581 ctgacaaatt gatgtaacgt catttctaac aatcatttta aatcataaaa gttattcgtc
     8641 cattgatttt gaagcttcga tttctgtttc agttaacttt ggtttaataa gtccataaat
     8701 taatgctgaa actaatgtac ctactacaag tgcaataagt gtttgtagta aatgtgcacc
     8761 atccgtacca acaattacga taataccacc gtgtggcgca gtaattcgag aacctaaacc
     8821 taaagcgatt gcgccaccta tacctgaccc aatcatcatt gaagggataa cacgtaatgg
     8881 atcggcagcc gcgaatggga tcgcaccttc tgtaataaac gacataccca tcacatagtt
     8941 agggataatt gaaccacgtt gttcttttgt aaatttacgt ctgaaaatta acatcgctgt
     9001 cgcaattgct aacggtggaa tcataccacc aatcattgca gctgtaattg gtgctgcatt
     9061 accttcaatc aacgcacctg ttgcaaaaac atatgccgct ttgttgaatg gaccgcccat
     9121 atcaatcgcc atcatagcgc caatgactaa acctaataat acaatattag aacctgataa
     9181 attatttaat ccatctaata ataaatgatt taaccaagat gctggtggat taaagacata
     9241 aatcatcaat aagcctgtag ctgtcactgt taataatggg taaattaatg ttggttttaa
     9301 tccttctaat gcttgtggta acttacgtgt catcacttta acaccttgtg ttaagtaacc
     9361 tgctaagaaa cctgcaataa taccaccaat aaatcctgaa ccacctgaaa ttgctaacat
     9421 accacctaca agacctgaag cgaatccagg tttatccgca atactacgtg caatgaatcc
     9481 agataaaatt ggaatgatta acgcgaatgc acttttacta ccaatgttcc aaagctgctc
     9541 tgcaaacgca ttgtactctg agctttttgg atcaaatgag tttgcaccaa ataaaaacac
     9601 gattgccatt aaaataccac cagatattac aagtggcaac atgttagaaa caccgttcat
     9661 taagtgttta tagaaagctt tacctgggct taatttttct tgactgtcat ttgagttacc
     9721 ttttctttga ccatcacggg caacaaaagg tttacgactt gtatctaatg ctttattaat
     9781 taattcttct gggcgtttaa taccatctgc tactggtact tctacgacat ttttaccatc
     9841 gaagcgatcc gtctcaacat gaacatcagc agcaacaatg atacctgttg catttttaat
     9901 atcttgttcc gttaaatggt ttttaatacc acttgaaccg ttcgtttcaa ctttaatttt
     9961 aatgcccatt ttatccgctt gctttttcaa tgcatcacgt gccatatatg tgtgtgcaat
    10021 acctgttgga caagcagtta cagctaatac atatggctcg ttagattgtt tagatgattg
    10081 ttctgcagtt gcaacttgtt gtgcttcagc ttctgcctct tcttcttttg tcgcttcatc
    10141 atcagcatga tcaataattg ctaacacttc ttctggagac gatgcatgta ataatttttc
    10201 acgtacattt tcatccatta aaataccaga caacttagct aaagcatcta gatgtgtttg
    10261 tgcgccacct tctggcgctg caatcataaa gaataagtgt gctggttgca tatccaaact
    10321 ttgataatct acgcctactt tagatttacc aaatgcaata gctggtgact taactgcggc
    10381 cactttggca tgtggaatag caataccttc accgatacca gttgtacttt gtgattctcg
    10441 attgtgaatc gcttccttaa atgacgcgat atcacttaat ttacctgctt tgtctaattg
    10501 atttactaac tcatcaataa caccattttt gtcatttgcc attaaatcca ttgctatcgt
    10561 atcttttgtt aataactctg ttactctcat tattttcact ccccatcaag tacgcaaatc
    10621 gtaacttgtg attttatttt ttctatagca tcccgtgttg ctaagtcctc atcaaatgcc
    10681 gtggcagtac cgcatgcgac tgcttgttga aatgcttttt caatcgataa acctgaagca
    10741 attccagcca ccatgcctgc aactgtacta tcaccagagc caactgtatt aaccactttc
    10801 ccttgtggat taactgcttt aatactgatt tctttatcaa tataaatagc accatcaccg
    10861 ccaagtgaga caataacaga ttgcgcacct ttatcaacta acaaacgacc atatttaata
    10921 acatctgcgt ctgagttcac tgttgtatta aacatcactt ctaattcatc tttattaggt
    10981 ttaataaata gtggatgata tggtaaaacg ctttcagcta attctttttc agcgtcgact
    11041 actaatttag cacctgtctg tgctgtaatt tgtgcaattt gtgcatacgc atcgcttgga
    11101 atactacttg gtacacttcc agcaacaata actatatctt cgcttgttgt atttttaatt
    11161 tgttgcaaca gttgttcaaa ttgtgttgac gttatatgag gacccggtgc attgatttct
    11221 gtttcttgtc ctgtttttaa tttcacatta atacgtgtat cttcatcaac ttcaataaaa
    11281 ttcgattgaa ttgcactgtt atttaatgta tctataatga atttcccagg aaatccacct
    11341 gcaaatccca aagcagttga ctcaacatcc aatgtcttta aaacacgtga gacattaata
    11401 ccttttcccc cagcgaattt atatgttgct gttgctctgt tcaaaccatc aattttaaaa
    11461 tcattcgtaa aaatgacata gtcaattgaa gggttgaaag tcactgtata aatcataaag
    11521 tccctcctat aaagtgatac ttttgtttgt attcttttaa cgattcttga tttaatgctt
    11581 tttcagatgt gatgattgtc gtactttcta gcaaaggtac acgagcaaaa tatactttat
    11641 taaacttaga atgatctata agtacaaatg attgattggc taatgacatt gctgtttgtt
    11701 taactaatgc ctcttgctca tcgggagtag ttaatccaag ttcaatatct aatccattca
    11761 tcccgataaa agctttatcg aaacaatatc gtcttaatat ctccatggca cttgaaccaa
    11821 tcgtagcaag tgtattttct ttaacttgac cacctagcat aattgtttta ataccttttt
    11881 taagtaaagc ttctacatgt gttaaaccat tggttaccac aatgatatct ttcgcttgaa
    11941 tatatttaat tagctccaat gtagatgaac cagcatcgat aaataagcat tcattatcgt
    12001 tgatttgatt agctgctatt ttagcaatca ttttcttttc atcgagatta gttgctaatt
    12061 tttcagttaa attcgcctca accatacgat tttcttttaa cattgcacca ccatgcacac
    12121 gttgtaattt ccctaattgt tgtagtttag ataaatctct tcgtattgtt gaagcactgc
    12181 agccagttcg atctattaat tcttgtaaag tcaaaaaatc tttgtgcgaa agttcttcta
    12241 atattaactc gtgtcttttt tctgtaatta tcatcatcac cccatctcac tctgtattat
    12301 aatgagtacg ctttcacttt tcaatcataa attaccaaaa acaatcacaa acaatcaaat
    12361 tgtgacgata aaattacatt tttatgttca aatactcaat tatcatgact gtttatgatc
    12421 gtaaaatgct atttcaatca aaaaggagtg tagcagaaat gaattttata aaaatttcat
    12481 ctctgtcgca ctcctaatct tgccccatac tttttgttgt tattaatcat tcatggataa
    12541 ctgctccaat tttcccttta gttattgtaa tcaaatcctc aacagctatc gtgatttgca
    12601 ttgttcgaag cccaccactc acactgatat gactataatt ttcacaggat ttgtcaacga
    12661 ctgttggaaa caatgttttc ataccaacag gtgaacaccc accacgaatg tatcccgtta
    12721 cattttttaa attatctaaa ggcataagtt gcaatttctt ctctccaacc atagcagctg
    12781 cctttttcat atctaaagtt tcacttactg ggataacaaa tacaaaatgt tcatgttttg
    12841 cattttctag aacaagtgtt ttaaatactg ttttaacatt tgcttgtatg agttctgcga
    12901 cttcttggcc agataaatgt tcttctggta cctcaaagct atgaacttcg tatttaattt
    12961 ttgcacgatc aagcatacgc atcgcgttag ttttcttatt tttagccatt tcaacacctc
    13021 atttatactt cttcagaact atattaacat tttaaaatac attacggatt ggttgatttt
    13081 gacataacat ttacatcatt ttataagttg atttcacctt tttaaattaa aatattgcaa
    13141 aataaaaaca gtatatccaa ttagaataca ctgttccatc tattatttcg ttattatgcc
    13201 ttgatttgaa atacttacat gttgcttact cgttgaatta tcattggaat aaacataatt
    13261 atatccatga tttgtcttag aaattctttc taaaccataa ttatcattga tatgcttatc
    13321 tgcataacga tcagcaataa cttggcactc ttgttcgtta aaagaatttg atttatttgc
    13381 attcacacga tgcttaaaat gattttgctt tttcacattt tcattcactt cagatgcttt
    13441 agcagtatga atattgagaa tatccatttt ctttttataa gtatcttcag ttgatacatt
    13501 tatcgcgtta agcgtattaa tcgttttatg taccttagca agcgcatctt catttgttgc
    13561 taatattaat aaaccaatta aacaaacggt taatattaca gcaacaaagc ctaaaaataa
    13621 attttttact gtcatcccct tacccacatt tccttactca ttttttatca tcttaacaca
    13681 ttaacgttaa tgaattaggt atgtggattg caatttaaaa agaatgtaat atttgtaaca
    13741 tgtttattac tcaaaatcat tttttgttac atataacatt taaatataaa gactaaaaca
    13801 tttaaaagtt tttaatcttt ataaatttaa agcatcgact tttcttcact ataattgtaa
    13861 gaaaatcgat gcttattttg aaatgcttta gttgtcgaaa tacattaatc gaatgacaac
    13921 catgtgcgat attacaaatc ttgttgtttt aacttagctc tatgttgctt ttcaattaaa
    13981 acaataacaa cacctgctaa tgacacacct atagccatat aaattggtgc ttcaatgtgt
    14041 acatcaaata acgcacctgc gattaaagga cctatgaaat tacccatact agtgaatgtc
    14101 gagtttaacc cacctgcaaa tccctgtcta tcaccagcaa tatttgagaa ataatttgta
    14161 atagcaggtc ttatcatatc gaacccgata aagacaacaa agctaattac cataattgtc
    14221 cagtaaccat cggcaataac taatagcact aatacaataa ctgaatatat taatgaccat
    14281 gcaataaatg ttaactcaga gaagtatttc ataaatttat caaagaaata aatttggaaa
    14341 agtgcaccaa agataccgcc acccgtaatc gcaatcgaaa tatctttagg tgaataattt
    14401 actttatacg atgtatataa agaatacagt gtttcaaatg ccgataaacc gaacgctaag
    14461 acgagcgtta aaattgctgg cgtaataaag actttccaat taatttttgt taataattgg
    14521 ggctcaagtt tttggaagcc gcttgtcgta gactttttcg gatcatgaat caatacaact
    14581 gacattatga aagctaaaat gcctaatgca cctgcaaaat aaaatggcat acgatgtgaa
    14641 acttctgcca taaatccacc aatccctggt cctaaaatga atccagaatt gataatcgct
    14701 gacatgtagc caaagttttt tgctttttga tggcttggtg aaacatctgc aatcaaacct
    14761 gtcacacccg gcattaccat accagcactc ataccaccaa tcactctcga taacatcaat
    14821 accgaaaaat tgtggccaac tgcaaacata aattctgaca ctgagaataa aattaaccct
    14881 atacatataa ttaatttctt ccctaatttg tcagctagcg taccaccaaa cggcgatata
    14941 atcatttgag ataacgcaaa agcagcaaca agtaacccta aatcactacc agttaatccc
    15001 aaatctttta aataaacagg caagactggt attactaaac cgatacctaa aaaaatcaag
    15061 aaaatattaa aatataagac aaaaatctgt ttattcatat gctcacctct tttttctcca
    15121 tgtcattctt aaatctgtta ttcttcttt
//
LOCUS       DATTQF010000055        14940 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.055, whole genome shotgun sequence.
ACCESSION   DATTQF010000055 DATTQF010000000
VERSION     DATTQF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.055"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            154..1152
                     /locus_tag="VB241_001419"
     CDS             154..1152
                     /locus_tag="VB241_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1244717.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1384..2265
                     /gene="rbsU"
                     /locus_tag="VB241_001420"
     CDS             1384..2265
                     /gene="rbsU"
                     /locus_tag="VB241_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HEP1244718.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2280..2684
                     /gene="rbsD"
                     /locus_tag="VB241_001421"
     CDS             2280..2684
                     /gene="rbsD"
                     /locus_tag="VB241_001421"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEP1244719.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2712..3626
                     /gene="rbsK"
                     /locus_tag="VB241_001422"
     CDS             2712..3626
                     /gene="rbsK"
                     /locus_tag="VB241_001422"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEP1244720.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3724..3858)
                     /locus_tag="VB241_001423"
     CDS             complement(3724..3858)
                     /locus_tag="VB241_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244721.1"
                     /translation="MIIIIINIAYLYAIIWKLTRLQKIVTSFITKHESSSIGLVENYF
                     "
     gene            3877..4638
                     /locus_tag="VB241_001424"
     CDS             3877..4638
                     /locus_tag="VB241_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP1244722.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5133..6569)
                     /locus_tag="VB241_001425"
     CDS             complement(5133..6569)
                     /locus_tag="VB241_001425"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEP1244723.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVNYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDFGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGRGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6585..7376)
                     /locus_tag="VB241_001426"
     CDS             complement(6585..7376)
                     /locus_tag="VB241_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEP1244724.1"
                     /translation="MGNKYKDYAQDILTAVGGVENIVNVSYDTVRMTIHMHHAIPSTA
                     NEVTQIDGVASVDENETQLVIVFNEEVKYVYQQLQLLMDDAKHQEDTNHDAVDTQETE
                     QKAQAKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     VSMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFECFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7523..8227
                     /locus_tag="VB241_001427"
     CDS             7523..8227
                     /locus_tag="VB241_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1244725.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSAGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8453..10744)
                     /locus_tag="VB241_001428"
     CDS             complement(8453..10744)
                     /locus_tag="VB241_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEP1244726.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHAELAVVKDKVMKEIRINRYSEKEDQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKVEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYEFDMKPTYESERELPK
                     IEVDSKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIA
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGKEPKKQGVLVDILFWALIIVVMGSMIGEW
                     GHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQAN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKKSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNVGLAGMVFITLMPVGYIQLKDALEHGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
     gene            complement(11085..11786)
                     /gene="lrgB"
                     /locus_tag="VB241_001429"
     CDS             complement(11085..11786)
                     /gene="lrgB"
                     /locus_tag="VB241_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEP1244727.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(11779..12222)
                     /gene="lrgA"
                     /locus_tag="VB241_001430"
     CDS             complement(11779..12222)
                     /gene="lrgA"
                     /locus_tag="VB241_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HEP1244728.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(12335..13075)
                     /locus_tag="VB241_001431"
     CDS             complement(12335..13075)
                     /locus_tag="VB241_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HEP1244729.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSANAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(13078..14832)
                     /locus_tag="VB241_001432"
     CDS             complement(13078..14832)
                     /locus_tag="VB241_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1244730.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRNVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
BASE COUNT         4892 a         2858 c         2332 g         4858 t
ORIGIN      
        1 caaccttaat cgcatactat aggcgtctgt ttgtagacaa taaaagtcat tcacgtcttc
       61 atatgtcatc aaatgtttat catgatatga tgaatataat aatcgggtat ataactgtat
      121 gattaattac acaataaaga gaaggaatat aatatgaaaa aagtgtcaat taaagatgtt
      181 gctagagaag ctggtgtatc agtcacaact gtgtcacata ttttaaatca taatgatagt
      241 cgtttttccg caacaacgat aaaaaacgta catgctgttt cagaacgttt aggctacgcc
      301 cctaacaaac atgcaaaaca attgcgcggc agtaaaattc aaactattgg cgtcatttta
      361 cctagcttaa caaatccgtt tttctcagca ctgatgcaaa gtattcatga ccataaacca
      421 tctgatgttg atttatgctt tttaacatct acagcaactg atttgtatga caatattaaa
      481 catttaattg atcgaggtat tgacggatta attatcgcac aatacatttc atccccggac
      541 gccctaaata actatctaaa gaaacatcat gtaccttatg tcgtactgga tcaaaatgac
      601 catcaaggct atacagattt tgttcggaca aatgaatatc aaggcggaca acttgcagca
      661 caacatttaa tagaactcgg tcacaacaat atgatgattg ttgcaccata tgacatgatg
      721 gcgaatatgt cgactcgtgt cgctggattt gttgatactt tgcgcgcgaa tcaattgcca
      781 gaaccacaaa tcgtccatac tgaattatct aagcgtggtg ggctaaccat tgttgatgac
      841 atcatggttc aatctgccac tgcaatcttc gctattaacg atgaactcgc tattggcatt
      901 ttacgaggac taattgaaca tggcatcagt atcccgaaag atatctcatt aataggttat
      961 gacgacattg attatgcagc gtacgtctcg ccacctttaa ctactgtggc acaacctata
     1021 actgatattg gcaaaacatc tttaacctta ttacttcaac gattacagca cttagataaa
     1081 tccattgata tgattgaatt accaacgact ttaaaaattc gtgcaacaac tggctatcat
     1141 ctttcaaact aactacgtat cttccgaaat atactcatca ttgttaggcc cttagcgttg
     1201 ctttaatgct gagggttttt aatcataatt atttcactat gaaattaaaa taataatgta
     1261 tgaattttca aatatgattt aaacgttttc agcttttatg aaaacgcatg cattttacaa
     1321 ataaaaatgg tacgatggca ctggtaaaac gttttactaa aaacaaatcg tgaggtgtat
     1381 aacatgagca ttgttgcatt acttatcggg ttaggcccct taattggctg gggcttcttc
     1441 ccaacagtcg cttcaaagtt tggtggtaaa cctgtacatc aaattatcgg tgctactgta
     1501 ggtacgttaa tcttcgctat tatattagcc gtagtcacat caagtggctt tcctactgga
     1561 accaatttgc tattcgcctt attatcaggt gcaggatggg gattcggaca aatcattaca
     1621 tttaaagcgt tcgaattagt cggctcatct cgtgccatgc cagtcacaac agcattccaa
     1681 ttattaggcg catctttatg gggtgtcttt gcattaggaa attggccagg cattggtcat
     1741 aaaatcattg gatttacagc tttagtcgtt attctaattg gtgcgcgtat gacagtttgg
     1801 agtgaacgca aagaagcaag taacgccaaa aatttacgtc gtgcagtggt acttctgtta
     1861 attggtgaat ttggatactg gttatattca gctgcaccgc aagcaacttc tattgatggc
     1921 ctaactgcct ttttacctca agcaatgggt atggtaattg ttgcagtcat ttatggcttt
     1981 atgaatatga aagcagagaa tccattccgt aataaaatta cgtggttaca aattatttca
     2041 ggtttcttct ttgcatttgg tgccttaaca tatcttattt cagcacaacc taatatgaat
     2101 ggtttagcaa ctggatttat tctttctcaa acatccgttg tgcttgctac attaactggt
     2161 atttatttct taaaacaaca taaaacgtca aaagaaatgg ttattacaat catcggctta
     2221 gtactcattt tagtagccgc ttctgttaca gtatttataa aataaggagt gtagatgtca
     2281 tgaaaaaatc agctgtttta aatgaacata tttcaaaagc aatcgcgaca attggtcatt
     2341 ttgatctatt aacaattaat gacgctggca tgccaattcc aaatgatcat cgtcgtatcg
     2401 acctagctgt cacaaaaaac ttaccgcgct ttattgatgt cttagctaca gtgttagaag
     2461 aaatggaaat ccaaaaaata tacttagcag aagaaataaa agaacataac cctacacaat
     2521 tgcaacaaat taaacaattg atttcatcgg aaatcgaaat cattttcatt cctcacgaag
     2581 aaatgaaaag taacttagct cacccattaa ataaaggtaa tattcgtact ggtgagacaa
     2641 cgccctactc taatattgca ttagaatcga atgttacttt ttaaaaatta taacttgaaa
     2701 ggagcataca catgaccaac aaagttgtta ttttaggttc aacgaatgtc gatcaatttt
     2761 taacagttga aagatatgca caaccaggcg aaacattaca tgttgaagaa gcacaaaaag
     2821 cattcggcgg aggtaaaggt gccaaccagg ctattgccac tgcacgcatg caagcagaca
     2881 caacatttat tactaaaatt ggcactgatg gcgttgctga tttcatctta gaagatttta
     2941 aagcggctca tattgatacg tcatatatta tcaaaacagc tgaagcaaaa acgggccaag
     3001 cctttatcac tgtgaatgca gaaggacaaa acaccatcta tgtttatggt ggtgcgaata
     3061 tgacgatgac acctgaagat gttattaacg caaaagacgc tataatcaat gcagactttg
     3121 tcgttgcaca attagaagta cccatcccgg ctattatatc tgcatttgaa attgccaagg
     3181 cacatggtgt gacgactgta ttaaatcctg caccagcgaa agcattacct aatgaattat
     3241 tgtcattaat cgatattatt gtgccaaacg aaacagaagc cgaattgtta tctgggatta
     3301 aagtaacaaa tgaacaatct atgaaagaca atgccaatta ctttttatct ttaggcatta
     3361 agactgtttt gattacgcta ggtaagcaag gtacatattt tgctactaaa aatcaaagcc
     3421 aacacatcga agcttataaa gtaaatgcga ttgatacaac tgctgcaggc gacacattta
     3481 ttggtgcatt tgtcagtcgc ttaaacaagt cgcaagataa cttagcagat gctattgatt
     3541 ttggtaataa agcgagctca ctcactgtac aaaaacacgg cgcgcaagca tctattcctc
     3601 tactagaaga agtaaatcaa gtttaaatga atcaaacaca gctatgatat gaaggtttag
     3661 catataacat gcaacattcg tatatcatgg ctgtgctttt ttatctttat aaaacatcat
     3721 ctattagaaa taattttcca ctaaacctat gcttgaagac tcatgtttag ttataaatga
     3781 agtgacaatt ttttgtaatc ttgttaactt ccaaattatc gcatataaat atgctatatt
     3841 aatgataata attatcaatt aaaaggaggt tatgttatgt ccaaagaagc tggtcataca
     3901 tttttagcta aattaggaaa aactcgtcta cgccccggtg gtaaagaagc aacagattgg
     3961 ttaatacaac aaggggcatt ttcacaagat aagcaagtgt tagaagtggc atgtaatatg
     4021 tgcacaacat ctatttatct agctcataca tacggctgtc acattcaagg cgttgatata
     4081 aataagaaag cattagaaaa agcacaggaa aatatttcag cagcaggtct tgaatcatat
     4141 attcaagttc aacaagcgaa tgctgttaaa ttgccctttg atgacaatca attcgatatc
     4201 gttttaaatg aagcaatgtt aacaatgtta cccatcgcca taaaggaaaa agcattacgc
     4261 gagtactacc gagtcttaaa gcctgggggt atcttgttaa cacatgatat tgtcatcgtt
     4321 aatgaatcac atgccacaca tgtagttaaa tcattatctg cagcaattaa tgtcaatgtc
     4381 tcaccgcaga cgaaacttgg ctggttagat ttatatcatc aagctggttt taatcatgta
     4441 cattatcata ctggtccaat gagtttaatg acaccaaaag gtttaattta tgacgaaggt
     4501 attgttggaa ctataaagat tatcaacaat gctttaaaaa aagaaaatcg accaatgttt
     4561 tgtaaaatgt ttaaaacgat gactaaattg cgtaaagata tgaattatat tacttttgtc
     4621 gctaaaaaag agcactaaat ataatgccac taactgtact ttatatctat gtttgactgt
     4681 tactttaatt tctttgtgac actaatcatc tacttaacaa tatcgttatc gttgattagt
     4741 aggtcatcaa ttttggttaa agacttttat aaatactcaa acattaacac tatacatact
     4801 tagttagtgg cattattttt ttcttaaaat tttaacatca cgggattagg acaaaaatga
     4861 tattttcgca aaatttattt ctgtccttcc ccaacttgca ttgcctgtag aatttctttt
     4921 cgaaattctc tatgttgggg ccccgccaac ttgcattgcc tgttgaaatt ggtgttccaa
     4981 tttctctgtg ttgggggcct gccaactcgc acattattgt aagctgtatt tccgccagct
     5041 tctatgttgg gggcccgcca atataacact ttagggccta ccatattaat ttatgtctcg
     5101 ggctctaacc ctaaaatact tataatgtac ctttataatt tatctccatt tgatgctatc
     5161 acttgttgat accaatagaa cgaatctttt ttcaagcgtt ccatcgtgcc acgaccatca
     5221 ttatctcgat caacatatat taaaccatag cgtttcttca tttcaccagt tgtaaatgaa
     5281 acaatatcaa tgattcccca cggtgtataa ccgattaaat ctacaccatc ttgatcaact
     5341 gcttctatcg ctgctgtaat atgtgctttt aaatattcaa tgcgataatc atcatgaata
     5401 tgtccatcta ccacttcatc aactgcacca aaaccatttt ccacaataaa aagtggcaac
     5461 tgataacgat catataacac attcaatgta tatcttaagc catctggatc aatcgcccaa
     5521 ccccaatcac tcgttgcgat atgcggattc tccacagaat gattcaaacc accgttgacg
     5581 atgttgtttt caactgtagt atcaacatca tgttttacag ccgtagacat gtaataacta
     5641 aagccaatat aatcaaccgt gccctgctgt aaaatactgt cgtccccttc ttgccatcca
     5701 aaatcatatc cttttcgtgc caacattttt ttagcatagc ttggataata acctcttact
     5761 tgaacatccg ggaaaaagaa acgtaagcga ttcgcaattt gtgcttccat catatctttc
     5821 ggatgacacg aatatggata aatgggtaca tgtgaaatca ttgttccaat cttaaatttc
     5881 ggattaatct ctttaccaag acgaaccgct aaagcactgg ctaaaagttc atgatgtgct
     5941 acttgataca agacttcttc aggattatca ttttctgtca atgctacccc agaattcgtc
     6001 cataaaaaga taggatttga tgtgtccatc tgattattta tttcattaaa cgtcatccaa
     6061 tatgtcactt tatctttata tctttcaaat acaacacgcg caaaatgcac aaaataattg
     6121 acaacttctc tatttctaaa tccaccataa tgtttcgcta aatgaagtgg catctcaaaa
     6181 tgtgataacg tcacaacagg ttcaatacct tgtgcaatta attcatcaaa gatacgatca
     6241 taaaaggcaa gtccttcttc atttggcaca tcttcatccc cattcggaaa gatacgtgtc
     6301 caagcaatcg acgtacgtaa acatttcaat cccatttctt taaacaaggc aatatcttcc
     6361 ttataacgat gataaaaatc aataccttca tgatttggat aatagtgatt gggatctata
     6421 gattctgtaa tctgacgtgc tttaccatgt gcaccactcg tcataacatc aattacactt
     6481 aaccctttac cacctttatc atatccacct tcaaattgat tggcggcaag agcgccaccc
     6541 cacataaaat tttgcggtaa ttttgtcatt attacaacac actcctagtt tttaaatatt
     6601 ttaaaaataa catcttcagt cgtaatgatt tcattcattg tcagttctac ttttcctaaa
     6661 tcggcagaat tgctaataac gacaataacg tcagcattgt agccttgttg ttgtatatat
     6721 tgctggtcga attgtaacaa cgtttgccct gcttcaacac gatcattttg ctttacaaag
     6781 cactcgaatc ctttaccttc caagtcaact gtattcacac caatatgaat gactatgtcc
     6841 acaccgtctt ctgattgaat accaactgca tgcttagttg gtacaatcat agatactaaa
     6901 ccattgaacg gtgcgattac tttggattct tcatgagctt tgattgccaa gccttcgcct
     6961 accattttct ctctaaaaat tgaatctctt acttctttaa gtaaaatacg acgaccagcg
     7021 attggtgctt tcactaaaat tggtgttgtc acttttgctt gcgctttctg ttctgtttct
     7081 tgagtatcca cagcatcatg attcgtatct tcttgatgct tggcgtcgtc cattaacaat
     7141 tgtaattgtt gatatacata ttttacttct tcattaaata cgataactaa ctgtgtttca
     7201 ttttcatcaa ctgacgccac gccatctatt tgtgttactt cattagcagt agaaggaatt
     7261 gcatgatgca tatgaatggt catgcgtacc gtatcatacg atacattaac aatattttcg
     7321 acacccccca cagctgtaag gatgtcttga gcataatctt tatatttatt gcccataagt
     7381 attaccccct caccttgata atagaatgga aatatatgac ttatcataat tataattgta
     7441 ccggttcaat ttgtaaacgc cgatacaatt ataatatttt gtgctataat aattacagac
     7501 aaagtgaaac gaggacagaa tattgttaaa gtatgaacat attgctaagc aacttaatgc
     7561 gtttatacat caatctaatt tcaaacccgg tgataaattg ccaagcgtga cgcaattaaa
     7621 agaacgttat caagtaagta agagtactat cattaaagca ttaggcttat tggaacaaga
     7681 tggtttgatc tatcaagcac aaggcagtgg tatttatgtg agaaatattg cagatgcaaa
     7741 tcgtatcaat gtttttaaga ccaatggttt ttctaaaagt ttaggcgaac atcgaatgac
     7801 aagtaaggta cttgttttta aggagattgc aacgccacct aaatctgtac aagatgagct
     7861 ccaattaaat gcagatgata ccgtctacta tttagagcga ttaagattcg tggacgatga
     7921 tgttttatgt atcgaatatt cttattatca taaagaaatc gtgaaatatt taaatgatga
     7981 tattgctaag ggctctatct tcgattattt agaatcaaac atgaaacttc gtattggttt
     8041 ttcagatatt ttctttaatg tagatcaact cacttcaagt gaagcttcat tactacaatt
     8101 atctgcaggt gaaccatgtt tacgttatca ccaaacgttt tatacaatga ctggcaaacc
     8161 ttttgattca tctgacatcg tatttcatta tcgtcatgca cagttttata ttcctagtaa
     8221 aaagtaatac aaacatcaaa acgtctatat tccagttata aaccggagta tagacgtttt
     8281 ataggacata atttctttaa aaacagtgag aagtacattt tcaatcagaa aattactttt
     8341 tactgttctt tatatataca taatctaaga acttatagtt tgaatcctat tgtataatat
     8401 ctcttcgtct caaacgttaa aacacactaa gatcactcag taataagatg atctatactc
     8461 tgtattttgg ttttaagtgt ctgtatcccc ttataaaaat aactactaat ataatgacac
     8521 ctactgtgaa tataatgtcc caaggcattc taccccacat gattgctttt acaagtggtt
     8581 gttcataaaa tgacgttagt cttgaatgcc aataaccatg ttctaatgca tctttaagtt
     8641 ggatataacc tactggcatt aatgtaatga acaccatacc tgccaagcca acatttaaag
     8701 cccaacatga tattttaatc catttttcaa ctttcggtgt ccaaaattct tttttagtaa
     8761 tatttttaaa aacatacaac gcaattgcaa ttgagaacat accatacacg ccagccatag
     8821 aaccatgggc atgtgctgca gtccattgcg taccatgttc aaagtagtta actgctggtg
     8881 tgttaattaa aaagcctaat gctccagcac caattgcatt ccacaatcct gttgaagcaa
     8941 gaaaaataaa tgtacctttg taaggaaaag cgatgtcact tttcttatat aagcgatagt
     9001 gtgtgtatgc ttcccaaatt aataaacaaa gtggcacgac ttctaatgct gaaaaacaag
     9061 aacccagtgc tagccatatg gaatgatcac cttgccaata gtaatgatga cccataccaa
     9121 caatacctgt acctagtaat aggattaatt gaaaatataa tgcacgaatc gtcgtttcaa
     9181 tggtagttaa tttcatatta accattaaga aaccaatcaa cactacggca aatgcttcaa
     9241 atataccttc aacccataag tgaacaatcc accatcgcca gtaatctgca aacgtcacat
     9301 gtgaattcgg cattataaat aatgatgcaa gataaaataa cggaattgca attgctccaa
     9361 caaacaatag cgtcaataag tgacgtttat catgattcgc ttgtactttg ttgcgaatag
     9421 ctggtaaaaa ccctcgacaa agaatgataa cccataagac catacctact ataaataata
     9481 tttgccaaaa tttacctaat tctatatatt cccaaccaaa gtgcccgaat agccaccaat
     9541 gagaatcaat ccaacctaaa atatgacccc attctccaat catgctgccc atcacaacta
     9601 tgattaaggc ccaaaacaag atatctacta atacgccttg tttctttggt tctttgccca
     9661 atactttagg tacaatgtat attcctgctg ctaaccaagt cgtagcaacc cagaaaataa
     9721 ctaattgcac atgccatgtc ttcgcaatat taaatggaaa gagcctttgt atttctatac
     9781 caaaaaattt attctctaca taataatgtg ccagtaattc gcctaataat atttgaacca
     9841 aaaatagtaa catgacaacg actaaatatt tcccaacttt tcgctgacta tcagtgattt
     9901 tactatctac ttcaatcttt ggtagttccc gttccgactc atacgtcggc ttcatgtcaa
     9961 attcataacg cttttgaata tatataatga atgccaagcc tgcaatcagt aatgttacag
    10021 atatagcagt ccataaaatt cctgcactag gcaacgtatt tcctgcatcc acatcgtatg
    10081 gccaattatt tgtatatgaa aatgtttttc caggtctatc tgttgatgat agccaagcac
    10141 cccagaaaaa gaattgactt aaatgttcaa ctttatttcc tgcgaccata taatcatcat
    10201 tttgaaactg tttaatcata ttttcattta accctacttg tttagggtta ttaacaaact
    10261 cttttttata ataatcttgc aaatatttta gaccgtatac ttgtcctggc gttaatacaa
    10321 gttgatcttc tttttcagaa taacgattaa tccgtatttc tttcatcact ttatctttaa
    10381 ctacggctaa ctctgcatga tttaatttat gaagttgctt atgataaata tcttgtgcat
    10441 aatatttacg cataccaatc aggtaatgat gtaatgcctc tgctgtataa tctgggccta
    10501 agtaggaacc attgcccagg taggagccat aatctgttaa cccatatttc tcatatatcg
    10561 cttgcccact gattagctcg ccttttgtaa caagcgtatg accttgttga tctactatct
    10621 ttgttggacg aggtgcttca tttttaaaaa tataccaacc acctactagt aaaattgaaa
    10681 acacaatcac taaaatcgtt agtagcactt taactaaacg tttatatgaa ctttgcatac
    10741 tcatttataa aatctcctct taattgcatt cttacatata agcatacgcc gtttttttat
    10801 aaagtgttga tgacattaag taatttcaag aatttgtcga aaaatattta attgaaattt
    10861 cacaaatgat ataacttaca ttttaattct tagttaaatt aattcgttct atcatttttc
    10921 aaaagccgca atacataaaa cgaacgccta aataccataa aacctagtaa aaagtaataa
    10981 atacataaaa acgtctatat cccagttata aaccggagta tagacgtttt tttacgataa
    11041 taacaatggc tcaaattgct attatcttgc ttaggttttt cgttttagaa gaatattgct
    11101 acaaagacag gcacaactgc tacaacaact acaccaacta acactaaagc tatacttgcc
    11161 attgattctt ctacaggtcc taattctttg gctggtgcta cacctaatgt gtgaccactt
    11221 gttccaagtg ctaatcctcg ggcaataggg ttagtaattc ggaaaagctt caagaattta
    11281 ttacctaggg cataaataat gacaccattt aaaataactg ctaatgatgt taattctttt
    11341 ataccaccga taccagctga tactggtaac gcaatcgctg tagttgctgc ttgaggtaac
    11401 attgataaaa taacatcatt ggcaaattgt gctaacttcg caaaagttaa aataattaat
    11461 aacgctacaa ctgtaccgat accaatacct ccgatgatac gatgccaatg tttaacaagc
    11521 acttcacgct ttttatataa cggaatcgca aaacagattg ttgctggttc taagaagaag
    11581 taaataatgt ctccacctat tttgtaagtt ttatacggaa ttcctgttaa atagaggaag
    11641 gccacaccaa ataccatact gacaaatagc ggtgcgaata agaagaaacg atttgttttt
    11701 tcaaataata tggttgctaa gaaaaatggt ataacggata acagtattcc gaagtaaggt
    11761 gtgtttagtg ctaagtggtt aatcatgagc ttgtgcctcc tctattttga tcttttttgt
    11821 gactttgtca cctttagatc tcgaagtaac tttcataata atttgtgtga catagccagt
    11881 acaaataagt aatagtattg ttgagacgat tattagtcca atgattaaaa atggtgcttg
    11941 gctaatgaca cctaaagagt taacaactga gataccggct ggtacgaaga gtaagccaat
    12001 gttatttgtt agtgtcgttc ctactttttc gacttcgcct aacttaacag caccagtaca
    12061 taataataca aataataata ctaaaccgat tactgatgca ggcataggaa ttggcataaa
    12121 tgattcaatt attttcgata caaagagtac taaagcaatt acaatgactt ggtgaaaaaa
    12181 gtgtgctggt tttgatgcgt ctttttgttg tttcacgacc attgcctcct acgtttgatt
    12241 taactaaagt atagatggct cactttgatt tgcgtgaatt ttagtccgaa atacaaaata
    12301 tcataggtaa aatgcataaa aaaaaggatt actgttaaag taatcctatc gacgctttaa
    12361 aatctttcat aaatgaacgt ccaacttgca tcttgacacc atttgtcaat attaccatat
    12421 aagtgtagtt aaaccattgt tgcacttctt taatgtgttt cgtgttaata atatatgaac
    12481 gatgaatacg tatgaaataa gcgggattca atcgtttttc ataacgattc aatggctctg
    12541 ttgtttcgta tttatgattc gttgtatgta tggttgtaat accattatgt gtgccaatcc
    12601 caataatatt ttgttgcttt aacatgtgaa ttttatcgtc aatttcaaca ggtaaacttt
    12661 gatcaaaatt cgccgacata tcattcgcaa ttgcatttgc gctattatca tctttggctt
    12721 tagtcgcacg aactttattg actgcttgtt caatacgttt ttgaccaaac ggtttcaaaa
    12781 tatagtctgt cgcatttaat tcaaatgcct gtactgcgta ttggtcatgt gcagttgcaa
    12841 aaataatcgc aggtggctct ttcatctttt gaattttagc tcctaattcg ataccatttt
    12901 catccattaa attgacatct aaaaatataa tgtcatattg atttatcagt agtgcttcca
    12961 atgtttcttt tacattttct gcctcattaa tttcttcaaa accaccaatt tcatttaata
    13021 aatatgttaa ttcattacgt gctaatggct catcatctat gattaatgct ttcatattta
    13081 ttcctcctct tgtctttcat aaggaagtac acaccaaaaa gtggtaccgc tcgatgtcga
    13141 ttcaaattgt aatgctgcgg attttccaaa taatcctttt aggcgtaagt ttaaattttc
    13201 taaagcacta ccagttccag attctgattc tacagatgtt tctcccaaca aatgcatttt
    13261 atctttagaa ataccctgac cattatcttg tacaataata cgtacatgtg ttgcagtttc
    13321 tttaatcact gacacgtcaa tatcgttacc ttgctttcga ttcgtaaacg catgtttgat
    13381 agcattttca actaaaattt gaattaaaaa tggtggtaca agcacattgc gatatttgtc
    13441 ttcaacatta atattgatat taaatcttcc tggataacgt gcttgttcga gtgataagta
    13501 tgcacgcact tgacttaact ctttatctaa agtaatcgta tgttgctttg agccttgtaa
    13561 attcgctctg aaaaaataac tcaattctaa tagtaactct cgtgcctttt cgctatttat
    13621 tctaactaaa gctgaaatcg tgttaattga attgaagaaa aaatgtggac tcacttgtgc
    13681 ctgtaatgac ttaatctcag catctttcaa taacttactt tgcgtttcgg cttcaccaag
    13741 ttcaatttgg ctactaaaaa tatttgccaa tccttctgca agttgacgtt ccacaaaagt
    13801 taaatcatta gggtttgtaa aatacatctt caatgtaccg acgatagaac catgcatctc
    13861 aagtggtatc acgatagctg ctctaagcgg gcaattcgga tgactacaac caatctcttc
    13921 tttagtatga acttctttca acttccctga tttcaataca tctttagaca gacttgttaa
    13981 tatttcattt gttggtatgt gatgatcact acctgcacct acatgcgata aaatttcatt
    14041 tttgcttgta attgctacgg cagatacttt cattaaattt ttaataatca tcgcaatttg
    14101 ctgtgccgat tctctattca atccttcttt aaaatacggc aatgtctggt tcatcaattg
    14161 cagtacatca tgtgtttgaa cagccttcat ttgctcctct tgctttaatg ttgaaatgat
    14221 aatagacata aaaatcgccg taccaacgct attaacaata atcattggta gtgcaattaa
    14281 tgatatgagg tcaaccgcat atgctttgtc gtgggaaaat gttaaaatgc tcaacatttg
    14341 aatcatttcc ataacaattc caatcatggc actttttgca atactcgggt aacgcttgcg
    14401 tctttgagct tgtaagccaa aataaccagc aattataccg ataaatatag atgatataag
    14461 ataaacttgt gcatccgccc cacccatata cactctgaaa atacctgaaa taacgccaac
    14521 aaatagacct acaaaagggc caccaactaa tcctgcgaca cctatcgtta atacacgtgt
    14581 gttagctaaa gatacatcat catctaaacg gaagtacaca cttcctgaca aactatgttg
    14641 atgatcgatg acgataccag ttaaattaga cattaaggca aacaaactga aaataataca
    14701 taattgccaa cgtgctttcc atgtacgtcg acgattcatt aagtttttaa aatatggaat
    14761 attcatcaac acataggcca aaataataat taaacctaca cgctcaagta ataacattgt
    14821 tagcgatagc acgatagcac ctcagtgaat tataattatg tttattttat cataatagtg
    14881 catgtaaaat tcatgaataa attattaaaa aatcacgcta cgatttaatt atctacagca
//
LOCUS       DATTQF010000056        14817 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.056, whole genome shotgun sequence.
ACCESSION   DATTQF010000056 DATTQF010000000
VERSION     DATTQF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.056"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            23..229
                     /gene="copZ"
                     /locus_tag="VB241_001433"
     CDS             23..229
                     /gene="copZ"
                     /locus_tag="VB241_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEP1244731.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSAEVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(319..1317)
                     /locus_tag="VB241_001434"
     CDS             complement(319..1317)
                     /locus_tag="VB241_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP1244732.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKDAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(1340..2494)
                     /locus_tag="VB241_001435"
     CDS             complement(1340..2494)
                     /locus_tag="VB241_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1244733.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSKIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKARRDRFEAMLTKADLPFFHAKGGIYVWLETPPGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQQLDEAAIRLTELAYLYE"
     gene            complement(2921..4429)
                     /gene="crtI"
                     /locus_tag="VB241_001436"
     CDS             complement(2921..4429)
                     /gene="crtI"
                     /locus_tag="VB241_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEP1244734.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTMCGKNYEDYIELRQLRYIYDVYFDRDDCITVPTDL
                     AELQHMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFADD
                     FRGNIEEIFEGRLSHDPSIYVYVPAVADKSLAPQGQTGIYVLMPTPELKTGSGIDWSD
                     EALTDQIKDVIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(4441..5304)
                     /locus_tag="VB241_001437"
     CDS             complement(4441..5304)
                     /locus_tag="VB241_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEP1244735.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEYHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVEKRKKAKL
                     FHEINSKYHRI"
     gene            complement(5341..6468)
                     /gene="crtQ"
                     /locus_tag="VB241_001438"
     CDS             complement(5341..6468)
                     /gene="crtQ"
                     /locus_tag="VB241_001438"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HEP1244736.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETACVARSYGATVVDVVDDADGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVEALINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMAAIVLWLLGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(6474..7967)
                     /locus_tag="VB241_001439"
     CDS             complement(6474..7967)
                     /locus_tag="VB241_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP1244737.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDDFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGTRVDNIKTYERCVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(7960..8448)
                     /gene="crtO"
                     /locus_tag="VB241_001440"
     CDS             complement(7960..8448)
                     /gene="crtO"
                     /locus_tag="VB241_001440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HEP1244738.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKHIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHKLNRNIYDQRHLMTINIDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRVVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(8629..9396)
                     /locus_tag="VB241_001441"
     CDS             complement(8629..9396)
                     /locus_tag="VB241_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1244739.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(9757..11568)
                     /locus_tag="VB241_001442"
     CDS             complement(9757..11568)
                     /locus_tag="VB241_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP1244740.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKSQFARTVLVILLLIPSI
                     IVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(12255..12956)
                     /gene="isaA"
                     /locus_tag="VB241_001443"
     CDS             complement(12255..12956)
                     /gene="isaA"
                     /locus_tag="VB241_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HEP1244741.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVPASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(13564..14619)
                     /locus_tag="VB241_001444"
     CDS             complement(13564..14619)
                     /locus_tag="VB241_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP1244742.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGILILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
BASE COUNT         4744 a         2848 c         2162 g         5063 t
ORIGIN      
        1 gctctaaaag gaggtgaatg ctatgtcaca agaaatttta aatgttgaag gtatgagctg
       61 tggtcactgc aaaagtgctg ttgaatctgc attaaataat attgacggtg tcacttcagc
      121 tgaagttaac cttgaaaatg gtcaagtaag tgttcaatat gatgacagta aagttgctgt
      181 atctcaaatg aaagacgcaa ttgaagatca aggttacgat gtcgtttaat taggcaatat
      241 tcaacgtcat caacaccaaa ttaaaaaatc gaactgatga gaatcccaac aatccaaatt
      301 atctcatcag ttcgattttt aatttactcg taacctagta tctccagtct gcaatacatc
      361 taatgttgca tctaatgcat caacaattag attttcaact gcagcttcag tataaaacgc
      421 aatatgtggt gttaatatga catcttccct gtcaatcaac gattctaaca atggatcgtt
      481 cagtgttttg cccctttgat cacttgggaa aagtttgcgt tcaaattcat acgtatcaag
      541 tgctgcacct ttaatcacac cattgtctaa tgcgtctaat aaagccttag tatctactaa
      601 agaacctctc gcacaattga caaatactgc gtccttttta aaatgtttaa ataattcagc
      661 attaaataga taatgattat atttcgttgc aggtacatgt aatgtcacga tatcagcacc
      721 ttcaaccgct tcctcaatcg tatctttgta atcgacatac gttgcaattt tagcattagg
      781 aaacgggtcg tatgcgacca catcactttg ataaccattg gcaaatatat cggctactac
      841 acggccaatt cgacctgtac caataacagc tacttttaaa tctttaatgg atttcgataa
      901 aatagtaggt tcccatctaa aatcatgctc tcgcactttc gtttgaattt gattaaaatg
      961 acgaaccaca ttaatagcct ggttcacagc aaactccgca attgaattcg gagagtatga
     1021 cggcacattt gacacaatca agttatactt gtttgctaac tccaaatcat atgtgtcaaa
     1081 tccagcacta cgttgtgcga tttgtttaat acctagttca tttaatcgtt tataaacatg
     1141 ctctgataat ggtatttgtt gtgatagcga taagccatca taaccagcga caccttcaac
     1201 attgtcatca gttaatgctt ctttagtaat atctacctca acatgatgtt tctctgccca
     1261 cgccttgata taaggcatat cttcatcacg tacactcatg attttaattt ttgtcatttt
     1321 aacatcaccc ttaactttat tattcatata aatatgctag ttctgttaat cttattgcag
     1381 cttcgtctaa ttgctggtca tctaacgcca atgagattct cacataacga ttaccattct
     1441 ctccaaatgg tttccctgga gcaacaagta ttgacttctc tttcactaaa aattgctcaa
     1501 attgctcgct gtcataacca ggcggtgttt ccaaccatac atatatgcca cctttagcat
     1561 gaaaaaatgg caaatcagct tttgtaagca tggcttcgaa tcggtcacga cgtgctttaa
     1621 atacattgct ttgttcttct aaaaaatcat cataatgatt caaagcatat attgcggcat
     1681 cttgtaatgc accaaacatc ccagcatttg tgtgcgtttg gtactttttc aaagcttgaa
     1741 tcatatcttt attaccaact gcaaaaccga ctctaaaacc tgacatgtta taacctttag
     1801 acaaggaata aatttcaatc gcaacatctt tcccattttc cgaagcaagt atactaggat
     1861 ttttagcgtc gaaaccgaag gcaccataag caaaatcatg cacgatttta gtgtctgtat
     1921 ctttaaattt agctatcgct tcatcaaaaa cttctttcgt agctgtcgat ccagttggat
     1981 tatttggata cgttaaataa atgagttttg ttttatctat tatttttgaa tcaactttgg
     2041 accaatctgg caaataatgc ggcggttcta aattaagcgg aacaggcttg ccatcagcta
     2101 aaagtacacc tgctaaataa tcagtgtagc ctgggtcagg tagtaataca tagtctcctg
     2161 gattgataac acatgttggt actgccacta atccattttt tgtaccatat aaaatacata
     2221 cttcatcttc tttatctaac gtcacattat attgtctttg ataaaaatct acaatagctt
     2281 gcttgaacgc ttctttacca tgaaaagcac catatttttg attttcagga atagttagtg
     2341 ctttttgaaa atgatcaata ataccttgtg gcgtgggccc atcagggatt ccaactgcca
     2401 tattaattaa tggcaatggt ccatgttcga ttttacgtcc catcgttttc ccaaaataac
     2461 tatcagggat atttgctaat ttgttagaga tcatcaaatt cctcctctat cattaaacat
     2521 agcctgggcg actatcataa tcctaacaac ttgtatcact ctcatttaga tggttacaat
     2581 gacatcgcca ttcaccgtta tcttcaacag atcttatgac acacgttgta ttgaatgaat
     2641 ttattttcat tttaggtagg tacaatatta ttgtcaatat taggaatttt cagattaata
     2701 tacgctcaat cgttaggatt taactgccat gcatatccgc atgcgcaacc agttagatat
     2761 gcttatataa agtataacgc ccatcaaggt acgtattcaa acgtgaacct taacaggcgt
     2821 cattcattgt taaataaaac ttcttaagca catacttatt tcactatgcc ttttacgttc
     2881 cccttatact tttctcacat ctttctctta gactactccc ttacacgcct tgctcaatat
     2941 ctttaatcat ttcatctaca gttattttcg cactcgttaa gacaatagga acgcctgcac
     3001 ctggatgcgt acttgcacct gcaaaatata aatctttata atctcgcgat acattttgtg
     3061 gacgataata attactttgc gctaaagttg gcattaaacc gaatgccgaa ccaaatttcg
     3121 catgatacgt ttgctcaaaa tcatttggcg taaagattgt ttctgaaaca atatgcgatt
     3181 ttatatcttc aaatacttca atagttgcta atttacgata aataacatct tttatttggt
     3241 ctgttaatgc ttcatctgac caatcgattc cgctacctgt tttaagttct ggtgttggca
     3301 ttaaaacata aatacctgtt tgtccttgtg gtgcaagaga tttgtcagca accgcaggaa
     3361 catacacata aatagaagga tcatgcgata atcgcccttc aaatatttct tcaatgtttc
     3421 ctctaaaatc atctgcaaaa ataacattat gaagtctcac ttgatctgtc acatcaatat
     3481 ctataccgat atacattaaa aatgctgaac aagagtaatc taagtctgca attttatgtg
     3541 gtggatactt tttaataggt gcaaaatctg gcattaatga ttccgcaaca ctagggaaat
     3601 cagccgtaca taaaatttta tcaaattttc ttatgtcacc attcactttt atcgcatcgg
     3661 ctcgtttgaa tttaggatca ataataattt gctcaatttc agcattaagt tcaatattaa
     3721 cgcctaaatc tttatttaat tgcgctagcc cttgagccat gccatacata ccgcctttaa
     3781 taaaatgcac accaaacatc atttcaatca taggaataat tgaatatagt gacgggcctc
     3841 gttttggatc aattcctatg tataacgttt gaaacgctaa aagcttttgt atcttttcgt
     3901 tatcaatata atgttcaatt agctgatctg catgatttaa cgtttttaac ttagcacctt
     3961 gcacaagtga cgtcatatta taaaagtcac ttggtttgcg atacgttctt tctaagaaat
     4021 agcgacgtgc aatttcatat tttttataaa catccgttaa aaaggacata aaaccatgcg
     4081 ttgaaccagg ttctatactt tctagcatgt gctgtaattc agctaaatct gtaggcaccg
     4141 ttatacaatc atcgcggtca aaatacacat cgtaaatata acgtaattgt ctcaattcaa
     4201 tataatcttc ataattttta ccacacattg taaatacatc tttataaaca tctggcatca
     4261 tgacaattgt ggggcccata tcaaatgtaa agccatcttt ctttaattga ttcatacgcc
     4321 cgcctacatt attatttttt tcaaatatcg tcacttcatg accttgagaa gcaatacggg
     4381 ctgccgctgc taatcctgtg acacctgcac caatcactgc aatcttcatt attcaaccac
     4441 ctatattcta tgatatttac tatttatttc atgaaacaac tttgcttttt tcctcttctc
     4501 cacaaaaaca cgttcatgta atgtatagtt agcctgtctc acttcgtcca gtatttcaat
     4561 atatatacgt gctgctaatt ctatgattgg ttgtgcttca atactaaata ctttgatttg
     4621 atccataaca tctcgaaaat ctttttctgc gatagctgca taatattccc ataagtcaat
     4681 ataatgatta ttaacaccat tttggtacac ttcagcaata tcaacttcat attgctttaa
     4741 tcgttgctta ctaaaatata tccgttcatt ttcaaaatct tcaccgacat ctcttaatat
     4801 attaatcaat tgcaacgatt caccaagcct tcttgcgaca tcgtaagtct gatgcgtttc
     4861 atgatcactt aaaatcggcg tcaatacttc acctactgta ccagcaacac cataacaata
     4921 tccgaataat tcagcgtccg tttcaaacat tgtaaaatgt tgatctttat atacagtatc
     4981 aatgagatta tagaaagatt gaaaggcgat atttttatgt tgtgcaacat gctgaagggc
     5041 catcatgatt ctacgatcac tttgaaagtg atgatattca tatgggtatt tttcaataga
     5101 ttgtatatct tcttttattt gatttaaaaa ttgaatatcg ccataaacat ctatactgtc
     5161 atcaatttta cgacacacag cataaattgc ccaaaccgct tttctttgat cttctggtaa
     5221 caagtcaaaa gcgtaagaaa agctttttga atgtttcttc atgattttat gacaatattt
     5281 aaaattcata tccatcattg tcatactagt cctcctatat tgaaatgcca ttaccttgat
     5341 ttattgttct ttagatatag aatattgacg acctttccat tcaactacac cataacgatg
     5401 cgtttgtttc caagattgga tgaaaatttt agtaaaaaac ataaataaca atggatgaca
     5461 taccattaat aaattagaaa attggccgac ccttcgatgc agataaataa attgtgtagt
     5521 atagctcaag taaactgtta acatttttcc tacagacatt tggcgatatt ttaaactaag
     5581 acatagccct aaaatactcg caatagaacc gagcaaccac aacacaattg ctgccatgat
     5641 cttaggcttt gtgccacctg ccccagtcga caaatgcttt gtccatcctt cttgtaatga
     5701 ctgaaatcct tcttgataca tgcgaaatgc aacaaatgga aatccttcat aaactgttac
     5761 gggcaatgat tgtgaagtat atgcacttcc taaagcaaat ccttcaataa tatgacggtt
     5821 cgcactttta tgacctccag ttgcataata atcttcttta tttgttaatg tcaccggtcc
     5881 aaatgcatgc tggttagtcc gaccgtcgtc taaggtagaa aatacattca taccaacgac
     5941 tgtcattaaa ttaaatattg ctgaaaaccc ttcgtagaaa cgctttgtta tatgataagg
     6001 ctgtacgctt aacaatcctt tttcaccttg taattgatac tgattaatca acgcttcaac
     6061 agcatctttc cttaagaaag ttacatcagc atctacaaaa gcaatacgat tcgtacatgc
     6121 atgcgtcaca ccttgataac aagcatgtga tttcccatac catttgccgt cagcatcatc
     6181 aacaacatct actacagtcg caccatatga acgcgctaca catgctgttt catctgtcga
     6241 tccgtcattc ataacaatga catctactgg aacttgctgt tgtattatcg aatgtagtaa
     6301 atgacctatt cttttttctt cgtttctagc cggaataata attgttaatg ctttatgatt
     6361 gaagttcagc gttttcgcct ttagctgatg tctacgatta aatatcaatg caccacacgc
     6421 catagacatg gtcactatta ctgttaatat tcgtgataac catttcatag atatcacttc
     6481 ctattcttcg cttctcgcgc gtttattttg tctgcgactt gttgcccact taatgtaacc
     6541 attggcatgc caccaccagg atttactgat ccccctacaa agtacaagtt ttcaaaatac
     6601 tgactttctt taggaaattt aaatcctttg tttttctttt tatctgctac aacaccatat
     6661 attgcaccac gattagaacg ataatttttt tcaatatcct ccggtgtcca aacatcttca
     6721 taaataatgt gttttcttaa atcagtgagt cccatttttt ctaatttatc caaaatttta
     6781 tccctaaact tcgcataatc ttcagttgta aaaggctggt cttgaatata tggaatatgt
     6841 ggtaagactt tgatattttc ataacctact ggcgcttgtg tgtgatcagt tttattcgta
     6901 tttactaaat aaatggtcgg atcatctggc aatacctttt catgaaaaac ttgttgataa
     6961 ttgagataag cattttccgt aaaaaagaaa ttatgatgtg ctaattgcgg gtattggcaa
     7021 gcaacaccta aatgcatcac atatcctgag cttgctggct caaattcccg ctctaatttg
     7081 tttaatcgtt gagtatcaag gtgaagtaaa tatttataag taggtatgac ttccatattt
     7141 gaaataatat aatctgcctt tacaaactca cctgtatcta atctgacacc cgtcacacat
     7201 ctttcatatg ttttaatatt gtccacacgt gtacctgtat gaattgtgac accttcttca
     7261 cgcgctagct tttccaaggc attggctaaa tgatggattc caccttctac ataccaaagg
     7321 ccttgctctt gttgcatatg gaataacata gataatacag ctggcgcatc gtacgatgaa
     7381 gaacctacat atttgataaa atagcctaac atttgtcgca agtatggatt cgagatacgc
     7441 ttgtctatgg cctgttgcat agtatgtaca taatcataat taataagtgc atttaatggc
     7501 ccatgaaatt taataatttg agatagtgta tctaaaccat agttaaaata ccctttttca
     7561 gtaatacgat cgattcgtct tgtataattc aaataatttt gcagttcctc tatatcctgt
     7621 ttcgacaata tcgcattatg ctgacctgtt tctttaatac cttcatacaa atcgatagtc
     7681 gttccatctg gaaaaaagct acgccattga tgtggcaatc gcttgattgt aacgtagtct
     7741 gacatttgct tcttgctata ttcgaataat ttttcaaaaa tataaggcat cgttaaaata
     7801 gatggaccta aatcaaagcc aaagtcatct gactcatgac gattcacttt gcctcctata
     7861 tgattatttt gttcatataa tgagaccgaa tagccacttt gtgccattcg aattgctgca
     7921 gaaatcccac ctaagccacc accaataacg atgatatgtt tagtcatgac gttcacctct
     7981 tcgttttaat atgcgtagta actgcgttaa tctcggtcga ttatagcgtt gcacaataat
     8041 gattggaaca ttagctatca ttgcatagaa aatatttata tactttacta cacgagggcc
     8101 tttattgaat ataatgactg gaagtatcga tatccaatga atcaactctg ccctctttgt
     8161 ctctataatc attttttcga tttcatcaat attgattgtc attaaatgac gctgatcata
     8221 tatatttcga ttaagcttat gaccatctaa aatcttatgc ttccattttc ttacgtaaaa
     8281 ccatttgttc caaaattttc catgcttctc aaagttaaat gatttaaata ttatatgctt
     8341 ctgacgaaaa tacttgtcag gaattcttgt acctaaattt gctatattta gttgaacaat
     8401 taaccaatac atactacaga aaaatgctgt tttgatatat tttttcatga ttttcatcta
     8461 aattgaatca ctctcaatca tactgacata gtataaatta tcattttatt acttagttcc
     8521 ctaaagatca gtgaataaac cgctctcatt tatatttata tttatataaa acaaaaacgc
     8581 caatactctc atgttagaga atattgacgt catttgattt acatttgatt aatgaatgaa
     8641 attatatgaa cctgcttggt ttgctgaaat tgtacgagat gtaaccacac cagcaccatg
     8701 tccatagttc atttctgaaa cacgaacaga accgttgctg ttaacgcctt caacgtaagc
     8761 aacatgaccg taatagcctt gtgttgtttg catgatagca ccagcttttg gtgtattgtt
     8821 cactgtatag ccagatgatg cagctgcgct agcccaatta ctagcattgc cccatgttga
     8881 accaattttc ccaccaacac gatcaaatac ataataagta cattgtcctg aagtatataa
     8941 gttacttcct gatgcataac cattagaaat tgaacgacca tttgaagatg gcgctgcagt
     9001 tgtagtcaca tgaacattat tgcttgttgt gctataactt gcacctgaac caccagtgta
     9061 atagttattt gttgctgttt ggctattgtt tgtatagtta ttgtattgat atgaatgatt
     9121 atagtagtta ttgtaagatg cattgttata actataagta ttgtagttat tgtaatagta
     9181 tgtgttgttt tgcgttaatt gacttggatt ccaatttcct gtccaagtgt aatgataatt
     9241 accttgtgca tcaattgtat acgtatagct gtatgattga gcgtcattag aattatatcc
     9301 attgttattt tgttctgcgg cttgtgcatc atgtcctgca aatgcgatag tggcaagtcc
     9361 tgctgtagcg attgtagctg taacgatttt tttcataatt aaatatcctc ccaataatca
     9421 aaacactcta tacatatagt cattagcgac tcaattattt taaattttgt attttttatt
     9481 tttttagtat gcaaataatt ataactttta tagttttgcg acaaaacaaa ctataacaga
     9541 gattaggtac cttgtggtct ttgttatcat aatgtaatct gttcctaaca aagcatcatt
     9601 gaatgtaata taccaattag aatgtttaca acactttttc tgcaccgtta acatgggatt
     9661 tgaaaataaa aaagcactaa acttttaatg aaatacatcc tttttgataa taaatatcag
     9721 aagtaatgta atatttcaaa agtttagtgc atcaaattat ttcttatttg tagcatgtgt
     9781 ttccatcgtt tttacaatca aatcagtcag cgctttacta cctgcatatt ctaagtgaat
     9841 accgtcatat gcaaagtact ccggatgacc tgcagaagct ttataccaat cgactagatg
     9901 tacattagag cgcttttcag ctgcctcata aattaattta tttattctac cttcataatc
     9961 tctaggtact ctaatagaaa ctaaatatat gtctgctttt ccaaaactat ccaatagttc
    10021 atttaattga tctttcgtaa atgccccatt tgtaccaagc tctactacaa ctttttgacc
    10081 ttttttagca tagtctttat attgcgattt cacaattggt gtagcatcaa cgagttgccg
    10141 tccaacttta ccatcaattt gtgcatttgg tattttctta gtaaagacat taccaatatc
    10201 caccataacc gagtcaccaa ttaatagtgg tgatgacttt ttaatatcct ctttactatt
    10261 cgccgtctgc ttatcctctt gcttatcttt tttaacgacc tttttcttcg ttgttttaaa
    10321 ttccgtcttc ttctctttct tctcagcttc atgttgttta ccaagcgcat caaattgccc
    10381 actgagaacg atgatagatg gaatcaataa taagataact aacacagttc tagcaaattg
    10441 actcttcttt ttaggtaaaa atgcaaaagc tttaaatcct tttttacgta taggtgtttc
    10501 aataaagcga tacgaaattt cagccattaa cgctgttaac aaaatttcta taatataaac
    10561 gtataccggt atttgtcctt gtacgtaata actgttcaca aaaacaatga taggataatg
    10621 ccataaatat aagctatatg atcgtttacc tataattagt aaaggtttca tacttaaaaa
    10681 tttagcaaat aaactagaag gatgtaccgc aatcgcaata atgaataaag ttgcaaatga
    10741 tataatgtaa aatcctccat tatagatcca ttgatcttgg tctccaacta taaagaacaa
    10801 agtcattaga accgcaaaac cagatatccc tataatatct aatgatacga caatcttttt
    10861 agaaatatct tttttcaaag caaacggagg ccaaataaat gctaatatac aaccaagcaa
    10921 taaagtttgc agtcgtgtat ctgtcccaaa atacacacgt gaattatctc cagtgatgaa
    10981 atgaatcact atcataagtc ctaaagaaat caacgataca ataaatagcg tttgaataat
    11041 atttctcggt ttaaatctat gtaataagaa cgtgataacc aatgggaaaa gcaagtaaaa
    11101 ttgttcttca atggctaaag accataaatg tttgagtggt tcaatagcaa attggttaaa
    11161 ataatctaca ttctgtgaaa tgtaccacca gtttgaaaca tagaatatag ctgcaatagc
    11221 atctcgtttc atttgtataa tcaattccgg tttaaatatc aaggtgaacg taagcacgac
    11281 acagattaaa aataacactg ccggaatgag tcgtttcaat cgtcgcttcc aaaactctag
    11341 caaatcgatt ttttgcgtcc gataatactc acttatcaac aaacttgtta ttaaataacc
    11401 tgaaataacg aagaatgtat ctactcctaa aaagccccca cttaaccatt gtgcattcaa
    11461 gtgataaata atgattccta taactgcgaa tgccctcaat ccatctaatc cgggtaagta
    11521 tcgcggggaa tacatttttt ctaaacgttt aaagtctttt gtatccatgt taataaacgc
    11581 cccatttatt tttctctatt ttgtagcata tcacaatatt tttgaaaata aaatattgca
    11641 ctgattttca ttaattgatt taacccttaa ttaagatagt tttaaatttt ttattaagta
    11701 gaaaacaatt attacagtta atttcattac tgcaaaccac atataaattt gtcgatttta
    11761 ctacataaca tagattatca tagattcttg aatttttagc aaaataactg ttattttcat
    11821 tatattttta caaaaaaagg ttcgttttat attttatgca tcttactgta acagaatcat
    11881 taagatatgc tattcggaat ttactttttc aaaatttcta taatgaataa attaccgcgc
    11941 actgttatta aaatatatac acggtgatat ctttccacct ctctttcaaa tttaaccata
    12001 tgaaatgaca aatactcact ccatattgaa gtatttgtgt aaaaagaatc atggtatatt
    12061 gtatatttcg ccttattcta atttaatagt aacattaatt cttctatcat gacattaaaa
    12121 tcgtcaggaa taataaaata acatcctaac ctatgaatca gactttaatt tctaataaaa
    12181 aaactctccc caatttctat gggaagagct atatatttaa tgtttaaaca ttacttttat
    12241 ttattatgaa ggaattagaa tccccaagca cctaaacctt gtgctttgta tgctttaaca
    12301 gctgcgttga tttgttggtc aacagtgttt gttgggcccc aacctggcat agtttggaat
    12361 aaacctgaag cacctgatgg gttgtaagca tttacttgac catttgattc acgagcgatg
    12421 attgcagccc atgtagaagc tggaacacca gtacgttgag ccatgatttg agctgctgat
    12481 gaaccagtag cacctgcagt attaccattg cttaatctca ctgaacttga agtagttgaa
    12541 gtgctgtagt tatggtaagt tggagctgaa acagcttcaa cgtttgagtt acttgattgt
    12601 gcattgtagc ttactgattg tacatttgaa ccttggttgt atgaagtagt gtagtctgca
    12661 cctgcaacgt ttgagaaacc agcagtttga ccattagctg cttcatagct ccatgaccat
    12721 gtagtaccat ttgaagtaaa gttatattgg aaaccatctt ttacaaagtg gatgtcatat
    12781 gcaccatctt tgattggagc tgcatttaat tgatcttgat gattatgcgc taagtcaact
    12841 aagtgtgctt gatcaacgtt tacttcagca gcgtgtgctt gatgtcctgt acctgctgcg
    12901 taacctgtta cacctaatgc cactgctaat gatgatgcca taattgtctt tttcatagta
    12961 aaaaatcctc cagtaataat tgtaagttta tgtttttagt aattatattt tgaatttgaa
    13021 tgtcgtagtg caagtttaaa ttgtctttta tttctttcaa cggtactcac tatatcataa
    13081 aaaagtagca agtaaattac actttcttta caaaacatta caatatcagg tgttatttgt
    13141 aatgttgaaa tatggttgtt ttatactgta atgtgaaata tgtttctttt agaatctaat
    13201 caatccttga aataagcata aatgcataag ttttttacta tatttagctc aactattaca
    13261 gctttcgtaa tattacagat tgtatttttg ttacacgcct gtaacattac cgtcatcaaa
    13321 tataacttaa attgattaat taaacacaat taaaaagcaa tcaatacata gattccctca
    13381 catgtattga ttgctattat tgttatatat tcaaagtttt aaaacacata tcttttgtgt
    13441 attgacttat cttttataag cacaaataaa ctgcagctca attatattgc taaactttat
    13501 tctcgcaatt cacaataaca ttaaataatt tttggtctaa tattttcaaa aaacatattg
    13561 tccttatccc atgaatttaa aaatatcatt agtatataaa cgaaacactt tacgataaat
    13621 gatatctgca agccaagctg ttacaaatgg tacaacaaag aacgctacta caattagtaa
    13681 gacactcaac caagcagaat caacctccat aaatttaaat gcattaatcg gtcctaccat
    13741 tcctataaaa ccaaatccag ctgactcttt cgttccatga atacctacta atgctgatac
    13801 caatcctgat acaatggctg tcgtcaatat tggtaacata agaattggat atttcaccat
    13861 attaggtatc atcattttaa cgcctccaaa gaagacggat aacggcaccc ctaaacgatt
    13921 tactttactt gtaccaatta tcaatactgc ttcagtcgcg gaaataccaa ttgacgctga
    13981 tccagctgct aaacctgtaa tacctatcgc aaaggcaatg gccacagttg atagtggcga
    14041 aataataata agactaaata ccattgaaat caaaatactc atgacaattg gttgtaattc
    14101 tgtaaaacca ttaaccatat taccgatggc tgttgtaatc attttcgtat acggcaatat
    14161 taaaatacca attgcacctg aaataccgcc aacaactgtt gggaatacaa tcaaagccat
    14221 actacctaca cgatgttgaa taagtaaaat gaacaacact gcaatcgctg ctgtaatcat
    14281 tgtattaatt aaatcaccaa tacccgtaat catccaagca ccatttttaa actgcgctgc
    14341 accgcttcct acatatgctg cacttgccac aacagcaatt gctaatggcg ataggtcaaa
    14401 tttcatggca accaatgcac caatcaaagc aggtactgta aattgaattg caacgacaac
    14461 gcctaataac gttttaaaaa tcggatgata atccataaag tatttaaaaa tttctccaag
    14521 tatcgcatta ggaactaaac ccgcaacaat acctatggcg acacctgata aaactctaaa
    14581 tataaaatct ttgggtgtaa ttgttttaat tgatgtcata atatcatcct tccatttatg
    14641 tatatacatc tttatgcaaa taataaagag ccttaagtta taagctgcca ctagcttaaa
    14701 ttctaagatg tgcatgccga tgttgttata tttaggctag cagtatcatc tataactcaa
    14761 gactatgaaa aatagtatat cacaaaattc tgaattttta gataaagaaa ttggcaa
//
LOCUS       DATTQF010000057        14439 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.057, whole genome shotgun sequence.
ACCESSION   DATTQF010000057 DATTQF010000000
VERSION     DATTQF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.057"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..2835)
                     /gene="cna"
                     /locus_tag="VB241_001445"
     CDS             complement(<1..2835)
                     /gene="cna"
                     /locus_tag="VB241_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HEP1244743.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEAFAARDISSTNVTDLTVS
                     PSKIEDGGKTTVKMTFDDKNGKIQNGDTIKVAWPTSGTVKIEGYSKTVSLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGPNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSLNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKAFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIKTDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     KDTTPEGYTKKEDGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGE
                     KVETVDVTSETNWKYKFKDLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGE
                     TSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWTGLDEKAK
                     GQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSISGEKVWDDKDNQDGKR
                     PEKVSVNLLANGEKVKTLDVTSETNWKYEFKDLPKYDEGKKIEYTVTEDHVKDYTTDI
                     NGTTITNKYTPGETSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNN
                     WTHTWTGLDEKAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSISGE
                     KVWDDKDNQDGKRPEKVSVNLLANGEKVKTL"
     gene            3213..4631
                     /locus_tag="VB241_001446"
     CDS             3213..4631
                     /locus_tag="VB241_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEP1244744.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDTVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDESSRKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(5180..6088)
                     /gene="rarD"
                     /locus_tag="VB241_001447"
     CDS             complement(5180..6088)
                     /gene="rarD"
                     /locus_tag="VB241_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEP1244745.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SVIFMIFILAVGQKQRNAFQRDMNQLLAKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            6310..6885
                     /locus_tag="VB241_001448"
     CDS             6310..6885
                     /locus_tag="VB241_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1244746.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEDVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVHSSLPFLLTGDVPCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGHFDIYGPQILIQELSNHYKEIASLNIQSIITHGYFGSMHGSQILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            7049..8065
                     /locus_tag="VB241_001449"
     CDS             7049..8065
                     /locus_tag="VB241_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEP1244747.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVIAALV
                     IGMIELLQILADKLDLHGAFWAFISSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            8307..9107
                     /locus_tag="VB241_001450"
     CDS             8307..9107
                     /locus_tag="VB241_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEP1244748.1"
                     /translation="MNIAKLEDYLQIEPTRYNQPSIEALNYYATRFMLTVPFENIDVQ
                     NGKPISVDIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     QNGSHASLVVSINNVFYVTDVGFGDLPLNAIPITLPDDTQQITDISGTFRAIFNSEDK
                     DIFYVQKYENNHWHTKYEAEFKAREIEEFDQNIEYNQTHPDSVFVKHLLITMPQSFGR
                     ATMFENHLTLTKDGTPEKLAITKDNYKQFLTKYFGLNVTISRLENSDN"
     gene            complement(9409..9921)
                     /locus_tag="VB241_001451"
     CDS             complement(9409..9921)
                     /locus_tag="VB241_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEP1244749.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            10211..10969
                     /gene="vraD"
                     /locus_tag="VB241_001452"
     CDS             10211..10969
                     /gene="vraD"
                     /locus_tag="VB241_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HEP1244750.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            10959..12839
                     /gene="vraE"
                     /locus_tag="VB241_001453"
     CDS             10959..12839
                     /gene="vraE"
                     /locus_tag="VB241_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HEP1244751.1"
                     /translation="MTFNHIVLKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFIITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTILML
                     IIAYILILFQSSLFLKRRSILSMMKDSVKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNDFNVVASQ
                     DAQQFESKLSQQHITFSKNYYETITVDNVKDQVITLENGSDSGRTNSILSANTKLTGN
                     NAIITNTKSLPNVINIHLNNDLVVKGTKNETFRVTQEDKDKVYPLNLIFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKKKAQAIAKQFGDKIITYDDMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIIVIIVYTLIYIVFALIAFVHSNKLIKK
                     TI"
     gene            12931..13122
                     /gene="vraH"
                     /locus_tag="VB241_001454"
     CDS             12931..13122
                     /gene="vraH"
                     /locus_tag="VB241_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HEP1244752.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAIIVIVLSTVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            13185..14141
                     /locus_tag="VB241_001455"
                     /pseudo
     CDS             13185..14141
                     /locus_tag="VB241_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(14209..>14439)
                     /locus_tag="VB241_001456"
     CDS             complement(14209..>14439)
                     /locus_tag="VB241_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1244753.1"
                     /translation="SADEKYVIATIKNIKNKDLIDYSLYIKKGLVEPFIVFYNQENIF
                     YISNAVIDIISVSKDTLGYIKSEFNDHCASPY"
BASE COUNT         4571 a         2523 c         2223 g         5122 t
ORIGIN      
        1 taacgttttt actttctctc cgttagccaa taaattcaca ctgacttttt ctggtctctt
       61 accatcttga ttgtctttgt cgtcccatac tttttcacca ctaattgatg ttgtttctgg
      121 cgtatattta ttcgtcacaa tcaagttgcc catatcattg ttatccacat gtgttgtata
      181 gcctttaact tttgttaatt cctcgactgt gtattttact tgttgacctt ttgctttttc
      241 atctaatcct gtccacgtat gggtccagtt attagattca tttaatgttg ccgtttttcc
      301 tgttgctttt ccatcttgat ataactcaac tttgatttca gttggtcgtt ttccgtcttg
      361 gttattattg tcatcccaat tttttgttac tgttgccgat gtctctcctg gtgtatactt
      421 gttcgttatt gtcgtaccgt tgatgtctgt tgtgtagtct tttacgtgat cttcggtcac
      481 tgtatattct attttctttc cttcatcata cttcggtaag tctttaaatt cgtacttcca
      541 gtttgtttca gatgtcacgt ctaacgtttt tactttctct ccgttagcca ataaattcac
      601 actgactttt tctggtctct taccatcttg attgtctttg tcgtcccata ctttttcacc
      661 gctaattgat gttgtttctg gcgtatattt attcgtcaca atcaagttac ccatatcatt
      721 gttatccaca tgtgttgtat aacctttgac ctttgttaat tcctcgactg tgtattttac
      781 ttgttgacct tttgcttttt catctaatcc tgtccacgta tgtgtccagt tattagattc
      841 atttaatgtt gccgtttttc ctgttgcttt tccatcttga tataactcaa ctttgatttc
      901 agttggtcgt tttccgtctt ggttattatt gtcatcccaa tttttcgtta ctgttgccga
      961 tgtctctcct ggtgtatact tgttcgttat tgtcgtaccg ttgatgtctg ttgtgtagtc
     1021 ttttacgtga tcttcggtca ctgtatattc tattttcttt ccttcatcat acttcggtaa
     1081 gtctttaaat ttgtacttcc agtttgtttc agatgttaca tctaccgttt ctactttctc
     1141 tccattagcc aataaattta cactgacttt ttctggtctc ttaccatctt gattgtcttt
     1201 gtcgtcccat actttttcac cactaattga tgttgtttcg attggttttt cagtattagt
     1261 aaccactaaa ccatcttctt ttttagtata tccttctggt gttgtatctt taccttgagc
     1321 atttacttct ttaactaaat atttaatagt cttgccattt ttgtcatttt ccggaagatt
     1381 agaccatgtc acttttgtcg ttccatcttc taatttttta atttctgctt tgtctactgg
     1441 tgttgtgttt tgattgtcat cttgtttgta caacttgaaa taaatcgttg gtttcacttt
     1501 ttgagtgcct tcccaaacct tttgagcaga aacatctttt gttttttcta tctcttttgc
     1561 attttcaata gtcgtaaaat atccctgatt atctgtatct ttcatagtaa acggatattc
     1621 tttatcctta tcaaatgtat acggtgctgg cgcctctatt tcttttaaaa tatagtctcc
     1681 gctaggcaac gctttaatat tagcaatacc gtttgcatct gttttaatct caatttcttt
     1741 ttgattgtcc tttacaactg atccatcttt tttagaaagt ttaaatttta cattagctat
     1801 aggagccttg gtatctttat cctgttttaa aacttttaat tcacctttta cagtaccttc
     1861 aataccggca ttagcattaa tattatgcac agtatgatta aatgctttcc cgttcacttc
     1921 ttccttacca tgctcttgat accaagcttg tgaattatta acaaactctt tttgctgttc
     1981 attcgtaatt ttggttttgt agttaattga aaaactatta agtgacccat agccttgtgg
     2041 aattgttaca tcaattgtgt tcttcgtatt atcaacagtt attttagaac ctggaaaggc
     2101 tttttcaaaa tcagtaattg catttggtcc actataataa ttgctatgtg tacctgtcac
     2161 attaatgttt aatgtgctta aatctaactg ctgtccacct tgaatctgat cctttatagt
     2221 aatatctttc gatacatatc ttttttcatt attaatattt aaaaaccatc gtacatgtgt
     2281 cgtatcttct ggcagcatat ctcccgtttt ataatagaaa acactacttg ttcccgcttc
     2341 acttttatga accgtaacat tcgttgattt attcccagat gttatcgtag ctactttgtc
     2401 atctgaagta tttgtttgcg ttaaatttct tccttgtact tcaaattctg caaatcccga
     2461 aacatcactt aatttttcta ctttatcatt gaatgtaatt gttgctccgt ctggcgtaat
     2521 aactgcttga cccacctgtt cacctttaac agttaatgat actgttttgc tataaccctc
     2581 tatctttact gtaccacttg tcggccatgc cactttaatc gtgtcaccat tttgtatttt
     2641 tccattttta tcgtcgaacg tcatttttac tgtcgtttta ccaccatctt ctatcttaga
     2701 cggtgataca gttaaatctg taacattcgt tgatgaaata tctcgtgctg caaatgcttc
     2761 atttttatta aataaaggtg tgatgatatt taataacatt ataaagacca taaacttcaa
     2821 cacatttttg ttcataaaaa cccctcctta tactctatgt catatccaca ggatactaat
     2881 aataaattat aaactaattt agttcttatt caatgaaatt tatcaaaaat tcatattatt
     2941 attttattat gcaaaatata tacagtataa cgaatacaag tgcatgaagt taacttgtat
     3001 caattaattt cttagtatat tagttcattt tcaaaatctt ttaaatttaa taaacaccaa
     3061 ctataccagt tcttcactgc ttctcgaaaa ataattatat taatgatttc gttaatttaa
     3121 ttttatattt aattattact gtacatcttt tgtagttagc tttattctta aattgaaata
     3181 tgtataaaac atagaaagaa ggacgtttta acatggaaaa cacggttaaa tatcgtaagt
     3241 ttatactccc tatcgttgta ggtctcctaa tttgggcact tacacctttt aaaccggata
     3301 ctgtggatcc aacagcatgg tatatgttcg caatattcgt cgcgacaatc attgcttgta
     3361 ttacacaacc gatgccaatt ggggctgtct ctataattgg atttacaatc atggtactcg
     3421 ttggcattgt tgacatgaaa acggctgtcg ctggttttgg taataatagc atttggttaa
     3481 ttgctatggc ctttttcatt tcaagaggat ttgtgaaaac aggtcttggt agacgtatcg
     3541 cacttcattt cgtcaaatta tttggtaaaa aaacattagg cttagcatat tctatcgtcg
     3601 gtgtagattt aattctagcg cctgctacac caagtaatac cgcgcgtgca ggtggaatca
     3661 tgttcccaat tatcaaatca ctttctgaat catttggttc gaaaccgaaa gacgaatcat
     3721 cacgtaaaat gggtgcattt ctagttttca cagaattcca aggtaactta attactgcgg
     3781 ctatgttttt aactgcaatg gccggtaatc cccttgcaca aaatttagca tctagcacat
     3841 ctaatgttca cattacatgg atgaattggt tcttagctgc tttagttcct ggacttgttt
     3901 ccttaattgt tgtacctttt atcatttata aaatttatcc accaactgtt aaagaaacac
     3961 caaatgctaa aagttgggct gaaaatgaat tagcgactat gggtaaaatc gctttagctg
     4021 aaaaatttat gattggtatt tttgtcattg ctctaacact atggattgtc ggaagtttca
     4081 ttcatattga tgcaacttta acggccttta ttgcgctagc attgttatta ttgacaggcg
     4141 tcttaacatg gcaagacatt ttaaacgaaa caggtgcttg gaacacatta gtatggttct
     4201 cagtgttagt gttaatggcc gaccaattga acaagcttgg atttattcct tggttaagta
     4261 aatccattgc tacaagtctt ggtggattaa gctggcctat agtactggtc attttaatat
     4321 tgttctactt ctattcacat tacttatttg caagttctac agcacatatc agtgctatgt
     4381 atgcagcatt actaggcgtt gccatcgcag ccggtgcacc accattattc agtgcattaa
     4441 tgttaggttt ctttggtaac ctattagctt caacaacaca ctatagtagt ggtccagcgc
     4501 cgattctctt ctcttcaggt tacgtgactc aaaaacgttg gtggacaatg aacttaatat
     4561 taggtttcgt ctactttatt atctggattg gtttaggatc actttggatg aaagtaattg
     4621 gtatatttta aaatgtttaa attagggagt gggacagaaa tgatattttc gcaaaattta
     4681 tttcgtcgtc ccaccccggc aaggttgact agaattgaaa aaagcttgtt acaagcgcat
     4741 tttcgttcag tcaactactg ccaatataac atcgtagagc atagaacatt gatttatgtc
     4801 ccagcctgtt tttaatttgt atttaaaatc aatctttgct aaatcaagac tccccttttt
     4861 aaaatacgtt tatcctttaa atcattgcgt acttcactga aaatttgtat aaagatttaa
     4921 gtcattacgt aacatcacat aaaatacatt tctatattaa tccgcttcat tgattaacat
     4981 tacttatgta tcataaatca tcaaacaaaa aacactttcg cttaaattca tttaattatg
     5041 aattcaacga aagtgcttta ttttatattt aatgtttcaa atttatacgg ctgtcactat
     5101 tactgcacac atacctcagt ctcaccttaa catagcacaa aggaagttga aaacatgtat
     5161 aattatctct cctcgcattt tatagggttt ttgcgaccgg atgtttcttc aatttaatgt
     5221 attgagaaag actatataac acaatacctg tccaaataaa tataaacgta attaattgat
     5281 ctatactaaa aggctctttg aaaacaaata tgccgagtac aaacattatt gttggtccaa
     5341 cgtattgaat aaatcctatt agcgaaagtg gaatacgttt tgccccggct gagaatagga
     5401 ttagtggtat tgccgtaata gcaccagaaa ataataacca aaatgatgac atgttcaatc
     5461 caaatgacat ctgatgttgc tgccataaat aaataacgta tatgagtcca gcaggtgcgg
     5521 taacaataca ttcaatcgta atactgctga tggcatcaat atgtactact ttttttaata
     5581 atccgtatgt accaaaggat aacgctaata taatagagac gattgggaat tctccaatct
     5641 tgagcgtcat atataataca ccgatgaatg cgaataaaat ggctagccat tcaaatttat
     5701 tgaatctttc ttttaaaaag ataagtgcga gcaaaatgct aacaagtgga tttatataat
     5761 aacctaaact tgtttgtagt acgtgaccgt tcgttacagc ccaaataaat gtaccccaat
     5821 ttaatgtgat aacataaccc gctacgacaa ttgctagtaa ttgaatgggc ttggctaaca
     5881 attgattcat atctcgttga aatgcattgc gttgtttttg tccaaccgcg agtatgaaaa
     5941 tcatgaatat tactgaaaat ataatacgaa aggctaaaat ttcaaatgcg cctattgcat
     6001 caacgaactg ccaatatata ggtagtattc cccacagaat gtaagcacta agtgctaaaa
     6061 atataccttt tttatattct gtattcactt acatacctcc ttactctccc aaatttttaa
     6121 catgttacgc tcacttagtt aagctaatat aacgatttta ctaataatat ttcgataaag
     6181 atatcatttt gtttatattt tccacattta ttcgccaacc actaaacaat attaatttta
     6241 taaataattc tgtacaaatc agggtatatt gccagaaaga ctaccaaaca acataaagga
     6301 tggatacaaa tgactttacc taaaattgga aagcctgcaa cacgtgcatt aaattcacaa
     6361 ggtatataca cattagaaga tgtatcacaa tatacaaagt catctctaat ggagatgcat
     6421 ggcgttggtc ctaaagctat atcaatattg gaacaagctt tatttcagca ccaactacat
     6481 tttaaaacgg aagttcattc atcattacct tttttattaa caggagacgt gccatgcaat
     6541 catgccccta aacgtcaaca aatgatagat tttatagttg ctacagcagc attagatatt
     6601 gaattattac gttcactggt tacaacagaa tttatttggt ctgtgcctgg acactttgac
     6661 atttatggac ctcaaatatt aattcaagaa ctatctaatc attataaaga gattgcttcg
     6721 ctcaatattc aatccatcat cacacatggc tatttcggtt ccatgcacgg tagtcaaata
     6781 ttaaaaactg gtaaagagat tcattttgct catttttttg aatttgaaaa tcataaaaag
     6841 gatgccaagt taagcaaagt gacatcttac attgttgttg gttaatgact caaattatat
     6901 atcgcaaaaa attcttttgc ttttacatgt atatttttat ataacaaata attttgcata
     6961 acatttttgt tatatgaaca cttattacta gagtgcacat tgaacataca gtatgattaa
     7021 atatcatgtt atgaaagaag gatatatttt gactgttttt aaaaatgagc gcttaagctg
     7081 gttaccatac atagctattg tcattttgtt acacgttatt gggtttagtt ttttatggat
     7141 tgctggaaaa gaccatcata tcttatttgg tatggggatt cttgcatata cattaggttt
     7201 acgtcatgca tttgatgctg atcatatcgc cgcgatagat aatacggttc gcaaattatt
     7261 acagcaacgc aaagatccat ctggtgttgg tttctacttt tcaattggac attcatctgt
     7321 tgtattttta atggctgttt ttttaggggt atctgtaaaa tgggctaaag atgaattacc
     7381 acatttccaa gatattggtg gaacgattgg tacactagta tcaggtttct ttttagtgct
     7441 tatcggtgtg ttgaatctaa ttattttaat ctctttaatt aacttgttcg ctaaattacg
     7501 tcgtgaacac attgaagaag ctgaagtcga tgcattactt gaatctagag gatttgtttc
     7561 tcgatttgta ggaccttatt tcaaattaat cacgcgtagt tggcacgtat tgccacttgg
     7621 ctttttattt ggacttggtt ttgatacagc tagtgaaatt gcgttactcg ctctttcttc
     7681 aggcgcgtca caacaggcca tttcatttat cggaatctta tctttaccaa ttttattcgc
     7741 atcaggtatg agtttgttgg atactttaga tggtgttgta atgaaatatg cctataattg
     7801 ggcattcttc aatcctattc gcaaaatcta ttacaatata acgattactg cgatatccgt
     7861 catagctgca ttagtgattg ggatgattga attgctgcaa attcttgctg ataagctaga
     7921 cttacacggt gcattttggg catttattag ttcaatcgaa tttgattatt taggctatat
     7981 tctagtcgct ttatttttaa ttacttggct tatttcgagt ttaatttgga agtttggtcg
     8041 catagaacac aaatggtcta aataaagtaa cttgtcttgc aatcatcttt ttattattgc
     8101 gtttttagac accctgcata ctgactcctt tagaatacgc tctcagtagt catctttaaa
     8161 tcgtttcttt tagagatgac ctcattttga gagcgtattt ttctatctaa aatagatctt
     8221 gtaaaatcca gtgctttatt atttaaaatt ttagcatttc tgtttacaat tctcttatag
     8281 tctttaatac aggaggcgat atactaatga atattgcaaa gttagaagat tatctacaaa
     8341 ttgaaccaac tcgttataat caaccgagta ttgaagcatt aaattattat gcgactcgct
     8401 ttatgttaac ggtgcctttc gaaaatattg atgttcaaaa tggtaaacca atatctgtag
     8461 atatcgatgc acttttcaat aaaattgttc atgataaacg cggtggtttt tgttatgagt
     8521 taaacacatt tttcaaagca tatttacaac aaaaaggatt taacccagaa ttaatgtcgg
     8581 ctaccattca tacacccggt ggcggtcgta gtcaaaacgg ttcacatgca tcacttgttg
     8641 tttctattaa taatgtcttt tatgtaaccg atgtcggctt tggagactta cctctaaatg
     8701 ccataccgat tactttacca gatgatacac agcagatcac tgatatcagt gggacattcc
     8761 gcgctatttt caacagtgag gataaggaca ttttctatgt tcaaaaatat gaaaataatc
     8821 attggcatac aaaatacgaa gctgaattca aggctagaga aatagaagaa ttcgatcaaa
     8881 acattgaata taatcaaaca catcctgatt cagtatttgt aaaacattta ttaattacaa
     8941 tgccacaatc atttggacgt gctacaatgt ttgaaaacca tttaacttta acaaaagatg
     9001 gaacgccaga aaagttagct atcacaaaag ataactataa acaattttta acaaaatatt
     9061 ttggattaaa tgtaacaatt agtcgacttg aaaacagcga caattaatca taaaaatacg
     9121 gacgtaactt actttaatgt aggtaacgtc cttttttaat acacatgttc gttaggatgt
     9181 gggaaaaaat gatacagcgc cactaacaat taatgattta gtggttctca cacattagcc
     9241 agagctcgtg tgtacttata aatagacatt catgcgtaaa acgcatgcat aaaaaatact
     9301 aatttctaaa gaaaaagtat ttctttattg acactctgtg caaacacaac aaaaaggata
     9361 tggcacaaac ttcatgccac acccttcttt tgaaccgctt cattttactt aagcaagtgc
     9421 atctacaaca taaatgacac ctacgaatac aaatgcaata gcaatcatcg tcgaaataaa
     9481 gacgataccc attaatggat taaataataa aatgacaccg aaaacaatac ctaataaatt
     9541 aaaaatcact gaaattagtt ttaagctacc actacctgaa aacgtaaata atccagcaat
     9601 agaactaaaa ataaaccaaa atgcaaacat gtagataaaa aatgccgagc tagcaccaac
     9661 attaaaaatg acaataagac caaatagaat atctacaatc cccataaaca gaatccaatt
     9721 ttggttacca ccaactaaag ctttggcttt tctacgataa acaatttgaa tcacaccatt
     9781 aatcaataca aataatccaa ttaaccatgt gattgcataa aagttttcaa ttgggaatgt
     9841 aaaaataaca actgctaaca ttaataacaa cacacccata atcaaactag accatttaat
     9901 tccttgattt cctttttcca taacaatcat ttcctttcaa acaacgcctt ctttatattt
     9961 taattagtgt cactattatt cattgtttaa attccctcat cttaacttat agaaacattt
    10021 cctcagtgca aatactgtga tgctcaaatt agttgaactg ctttccaata atccaaattc
    10081 acccgagaaa atcacgatat attaatatct ttcaactatg taagattcaa acattgaatt
    10141 gtaagtttag ataaaggcta ctgttctact tattatttaa aatgaattta aatacaaaag
    10201 gagtgatact atgacgatat tatcagtgca acatgtttca aaaacatacg gtaaaaagca
    10261 cacatttcaa gcacttaaag atattaactt tgacatacaa aaaggcgaat tcgttgcgat
    10321 tatggggcct tctggatcag gtaagacaac cttattaaat gtactaagtt caattgacca
    10381 aatttctagc ggtagcgtga ttgctaacgg acaagagctt aataaactta atcaaaaagc
    10441 acttgccaaa ttccgtaaag aatcattagg tttcatcttc caagattaca gtattctgcc
    10501 gacattaact gttaaagaaa acattatgtt acctttatct gttcaaaaaa tgtcgaaagc
    10561 aacaatggaa gaaaattata aagcaatcac aacagcatta ggtatttatg acctaggaaa
    10621 taaataccct agcgaattat ctggtggtca acaacaaaga actgcagcag cgagagcatt
    10681 tgttcacaaa ccacaaatca tatttgcaga tgagccaaca ggcgcactcg actcgaaaag
    10741 tgcaaatgat ttattacaac gtttggaaga aatgaataaa tcgtttgata ccacaattgt
    10801 catggttaca catgatccgg ttgcagcaag ttttgcagaa cgtgtcatca tgctaaaaga
    10861 tggccaaatt catacacaac tttatcagga aggacgttct aaacaggcct tttatgaaga
    10921 cattgtacat cttcaatcag tattaggtgg tgtctcaaat gacatttaac catatcgttt
    10981 taaaaaactt acgtcaaaac ttaaaacatt atgcaatgta tttattttca ttatttttta
    11041 gcatcgtctt atatttcagt tttacaacct tacagtttac taaaggtgta aataatgacg
    11101 actcgatggc tatcattaag aaaggtgctt tagtcggatc aatattttta tttatcatta
    11161 ttgtcatctt tttaatgtat gccaatcatt tattcgtgaa acgtcgtaca cgtgaatttg
    11221 cgctatttca gttgattggt ttaacacgac aaaacatttt gaaaatgtta gcacttgaac
    11281 aaatgattgt atttataatc acaggtgttg tcggtgtatt atgtggcatt gcaggtgcac
    11341 aattattgct ttccattgta tctaaattga tgtcattatc gattaactta tcgatacatt
    11401 tcgaacctat ggcccttgtt ttaactattt taatgctaat tattgcgtat atactgattt
    11461 tatttcaaag cagtttattt ttaaaaagac gcagtatctt atcaatgatg aaagactcag
    11521 ttaagactga tgttacaact gcgaaagtta cagttataga agtcatttca ggcgtattag
    11581 gtattgctat gattgcacta ggatattata tggcgacaga aatgtttggt acgttcaaag
    11641 cactaactat ggctatgaca tcaccgttta tcattttatt tttaacggtt gtaggtgcct
    11701 atttattctt cagaagttcc gtgtcactaa tttttaaaac tttgaaaaaa tctaaaaatg
    11761 gacgcgtatc tattacagat gttgtattta catcttctat tatgtacaga atgaagaaaa
    11821 atgccatgtc tttaactatc attgcaatta tttctgcagt tacagtaact gtattgtgtt
    11881 tcgcagcatt atctaaatca aataccgatc aaacccttac atctatggca cccaatgatt
    11941 ttaacgtggt cgcctctcaa gatgcgcaac aatttgaatc taaactaagc caacaacata
    12001 ttacattttc taaaaattat tatgaaacta ttactgtaga taatgttaaa gatcaggtta
    12061 ttactttgga aaatggtagt gattcaggtc gcacgaattc tattttaagt gcaaatacaa
    12121 agttaactgg taacaatgcc atcattacaa atacaaaatc acttcctaac gtgattaata
    12181 ttcatttaaa caatgattta gtagtaaaag gtacaaagaa cgagactttt cgtgttacac
    12241 aagaagacaa agacaaggtt tatcctttaa atctaatctt taattcacct gtcattgaag
    12301 taagtccaga aaagtatcag caattgaaaa cacaaaataa cgttcatact ttttatggat
    12361 atgatattaa acaaacatca caaaagaaaa aagctcaagc aattgcaaaa cagtttggag
    12421 acaaaatcat cacttatgat gacatgaaaa aagaggtcga tgctactaac ggtattctaa
    12481 tattcgttac ttcattttta ggattagcat ttttagtagc agctggatgt attatttata
    12541 ttaaacaaat ggatgaaact gaggatgaac taagtaactt cagaatatta aaacgcatag
    12601 gctttacgca cacagatatg cttaaaggat tattactaaa aattactttc aacttcggtt
    12661 taccattatt gattgctata ttacatgcag tattcgccgc aatagcattt atgaaattga
    12721 tgggtaatat atccttcatg ccggttatta tagtaatcat tgtatacaca ctcatttaca
    12781 ttgtttttgc tttaatcgct tttgtacatt caaataaatt gattaagaaa accatttaac
    12841 acttgtgatt aaaagatgat tcattgagat tttgatttta agcaatgcag tataactatt
    12901 aactgattta taaaaaggag agattgaatc atgaaattta gagaattagt aaaacaatca
    12961 tacgaggatt taaaaaactt gactgtaact tggtataacg ttattgcttt agcaattatt
    13021 gttattgtct taagcaccgt tacaacacca attataggta ttccagctgg tttattaggt
    13081 ggcgcttatt atttaaaacg ccgcgaagaa aaaggtaaat aagccttctg tgctaaactc
    13141 aatatgtatt tcctaccaaa gaaaacataa ggagttagta cagaatggcc tatacacacc
    13201 ttaacacgga tgaacttacg ttcatagact catattatca ctagaatctt tcagttaaag
    13261 agatttcgag tagattgaaa cgttctagac agacaattta taacgtaatc aatgctttta
    13321 agtcgggtat gtctgccctt gaatattatc aagaatacaa acaaagaaag tctaattgtg
    13381 ggagacatag aatagtgctt ccagaagaac agtcagatta tatccgagag aacgtagctg
    13441 atggatagac acctgatact attattggca gaggtgaaca cacgatcaat tgttctgtta
    13501 aaacacttta cagaatgttt aaagagaaca tttttgctgt acatgattta cctatgaaat
    13561 gcaaaagaaa gccaaatggc cattctgaaa aaagaggtgg atagactttt aaaagacata
    13621 tttctgaaag aatagaagag ttccctttat tcaataagga gtttggacat ctcgaaggag
    13681 atactattgt tggtcgtcat ggtagtgcaa tcattacttt ggtagaaaga gtatccaaga
    13741 tgattatcac cttacgatcc ttaggtcgta aggcaactga tatagaaaat gctctgaact
    13801 caatgttttc tgctttacct tctcatatat ttaaatcaat tacttttgat tgtggtaagg
    13861 agttctcaaa ctggaaaaca atatgtaact gtcacgatat atctatattt tttgctgatc
    13921 caagcatgcc agcacagcga ggtttaaatg aacattctaa cggaatactt agacgcagtg
    13981 gattagataa ggaactggat ttcaatttag taacggatga tcatatcatc agtgttgctc
    14041 aaaaaatcaa tcatcgtcct agaaaatctt taggttatag aacaccattg gaagtattta
    14101 tgagtttcat caaagatgat aaactgtcca gcttaatttg acaatctaaa gatttataaa
    14161 aaagccgttg gtctcttcga aactacggct tttctctgtg ctataaaatc agtatggcga
    14221 agcacagtga tcattgaatt ctgactttat ataaccaaga gtatctttgg atacactgat
    14281 gatatctata acagcattgc taatgtagaa tatattttct tgattataga atacaataaa
    14341 tggctccact aaaccttttt taatatataa agaataatca attaagtctt tgttttttat
    14401 atttttaata gttgcaataa catacttctc atcggcaga
//
LOCUS       DATTQF010000058        14430 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.058, whole genome shotgun sequence.
ACCESSION   DATTQF010000058 DATTQF010000000
VERSION     DATTQF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.058"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(191..1084)
                     /locus_tag="VB241_001457"
     CDS             complement(191..1084)
                     /locus_tag="VB241_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1244754.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKSNAPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWEGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            1332..1625
                     /gene="esxA"
                     /locus_tag="VB241_001458"
     CDS             1332..1625
                     /gene="esxA"
                     /locus_tag="VB241_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HEP1244755.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            1708..4737
                     /gene="esaA"
                     /locus_tag="VB241_001459"
     CDS             1708..4737
                     /gene="esaA"
                     /locus_tag="VB241_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HEP1244756.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDSD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNNLWNNAITSGVIGATGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            4737..5195
                     /gene="essA"
                     /locus_tag="VB241_001460"
     CDS             4737..5195
                     /gene="essA"
                     /locus_tag="VB241_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEP1244757.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIVKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            5167..5409
                     /gene="esaB"
                     /locus_tag="VB241_001461"
     CDS             5167..5409
                     /gene="esaB"
                     /locus_tag="VB241_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HEP1244758.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            5422..6756
                     /gene="essB"
                     /locus_tag="VB241_001462"
     CDS             5422..6756
                     /gene="essB"
                     /locus_tag="VB241_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEP1244759.1"
                     /translation="MVKNHDPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKEDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            6778..11226
                     /gene="essC"
                     /locus_tag="VB241_001463"
     CDS             6778..11226
                     /gene="essC"
                     /locus_tag="VB241_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEP1244760.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGSNAYD
                     DMVIQSLMNAIIIKDFQSIQETQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNVFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIILSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVFMEDIREA
                     YDLEKIIHEEHKLPLGLDFEDVELVSLDLTSSSIVTAIKPTEMEKMNDVIMSSLSVYS
                     KNQFVILVDAEDNMSQYSEDVTSYYSAPSDLSNIRLGFKQEIEARKNGEKSIEECKIV
                     FINNIKRFNQLTGMTEDEIRVLFNEGQKVNIIIIASGLYSDTIGAFDRESKMMVRTIN
                     QALISHKISEQEFIRVKDRFGEPELKVGEMYYINNQEYQKIKLMEG"
     gene            11229..11495
                     /locus_tag="VB241_001464"
     CDS             11229..11495
                     /locus_tag="VB241_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwqI/YxiC family protein"
                     /protein_id="HEP1244761.1"
                     /translation="MIKLNQASVSKEISSIRTNGQGLKQSNGNVNLSKTNLVTFKEYV
                     NMFEDYQSALSNYENIIEQDTTAMDTTVTEIVENDREIAGQINK"
     gene            11518..11913
                     /locus_tag="VB241_001465"
     CDS             11518..11913
                     /locus_tag="VB241_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HEP1244762.1"
                     /translation="MSNKGEIRRQIANKEREKASKEAQLTDLKEDLRRLKDASKKLDT
                     AGEDFNKGQSSYNKVEISTSDWKGERRTKSDSKKKDVDSELKKVEQDFDDAKKAIKKD
                     IQDKEEEIKGVEGEISTINAAIDALKSKL"
     gene            11935..13605
                     /locus_tag="VB241_001466"
     CDS             11935..13605
                     /locus_tag="VB241_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1244763.1"
                     /translation="MGYKVDMSEVHNMQKSIDSSLSAINTKVSTLSSSINNLINTEGF
                     EGQTASSVKNYSKTFHLQSIKKIETINKDFKSDIAKSIRKFQSEVDNNASAILDEDEI
                     KKYKKDIDDALKDVFKSSKDANGAISDVSDLTTAKKIKTENLANKMGDFNKDIDQTVE
                     RLTTFDANNSIDGDKTDNLITELSGVNSYVKNMKPNRARISSTSGKIEGAIARHKTSE
                     ELVRWQTYMETLSENLYKTPGLSKAASDIITQAGREYYAIKAVGNGSALRGYKEYLKT
                     RDINKLINKMNKNQLKRMSLVLNTDRGNIKIKNAVKMASEFARNNPFKKGNLVNWMSK
                     VQGYQSVSAQILKKGLQDKNFKYTFGDAKKFFDTADMKKAAVTEFKNTFVPQNIRETF
                     FKKENLTSKTALKKNMSKFWNEDIKGGIKEFKLDFANKNIIGKLGKLLKLGGKALKPL
                     GFITAITDNIGKKSWQEKLVGMGVDLSAIGASAAAGAAVGTAIPLPVVGTVAGALAGA
                     AVGIALDYKIPYLDKSATELAKDGINSGINKVKSGFGKVTSGFKTVFG"
     gene            13621..14265
                     /locus_tag="VB241_001467"
     CDS             13621..14265
                     /locus_tag="VB241_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HEP1244764.1"
                     /translation="MKIQEIVGNIKRAYLTPLIIFVILFSLFFDLIMYFYGNLQGKLP
                     IYLDVFLIFAAVVFILMYFQEKSGEVKVEKSNVIRYLTLNVVAGYSMVLLVASIYVFG
                     VAGYGFDVFNYWLGIILMLFVSWFALFLFYKNEFDSENPNKTVNVIAIIIKLSAFGGL
                     FYIRTVVPNTADEEKFITLSILINIAVDLLLVRSYFNYALYKSVKKDIENGVDD"
     gene            14265..>14430
                     /locus_tag="VB241_001468"
     CDS             14265..>14430
                     /locus_tag="VB241_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HEP1244765.1"
                     /translation="MEVGMLIMPFCVKITFEIDKNDNWLNALDTVNEFFMDKDIYPTG
                     PIIFQRELAGL"
BASE COUNT         5613 a         1943 c         2660 g         4214 t
ORIGIN      
        1 acaaaaaata gtttgattac ataatgattc ttgaaaatgt cggttaactt taaggtgaca
       61 agttattgca attttgaagg tagacatcat attttataat cgaataacat acaaaataaa
      121 gcgaccaatt ttgcagtgtt acgaattgta aactgcgaac tggtcgcttt ttattgatgt
      181 catgattaat ttaatggatg taattatatg atgaaacttc tgaagcagag atggttcttg
      241 atgaaacgat atattcacca atccagttca tttctgaaat tagaatactt ccatcaatat
      301 taactttctc aacgtaggct acatggccaa atggaccgtt tactgtttgt aaaattgatc
      361 ctcgtgttgg gtgtctatct actttgaagc cattgcttga agcttggcct tcccagtttt
      421 tagcatctcc ccaaaatgta ctaatcgtgt gtccatcttt ggcacgttta tcaaagacat
      481 accatgtaca ttgtccagca gtatataagt tgttcttact tgtgataaga ggctgatcaa
      541 tgattttacc gttacctaaa gctaaaggtt taccgtcagc agtctttgct ttgtcattaa
      601 attcggcgat ttgtaattcg tcatacaatt cgtctaaatc gatgcctgta ataagccctt
      661 tgttatcttt tgaaaaagcg ttatttaatt catcgtcatt gtcttcaaca ttcggtattg
      721 ctggtgtcaa aggattgctt ggtgacgttt gaggcggtgt gtgtgaatca attgcgtcat
      781 taatgtgcgt atactgacca cttaatgaag aatggtactg attgttgtta agatcacgtt
      841 gatttgcgtg ttgatgattg tcgtttgtac gtgactgatt ttgatgattg ttgtttggcg
      901 cgttgctttt gtcataagta taagtatacg cgccggtgtc tttattcact ttgaactgtg
      961 cgtttgggtg tgctttcttt gcttcttcta atgttttgct atcattcgtg tatgcttgag
     1021 ccgagttagg cgacatacta aataaagtaa gagttgtcat cgtcagtaaa attgttttct
     1081 tcataataac catttaatcc tttatgtatt taatttaatt ttagtataca catttatatt
     1141 acaaaaatga atggttaatt aaaaatatat gggtatattc aatatattta tttaaaaaaa
     1201 gctaaaaata cttaaaaaac tatatacata tactaataat ttatatatta tttgagtaag
     1261 gatcactttt tcaaaaaata gtgtccctaa aaagttttga taaacttaaa atattcagga
     1321 ggtttctagt tatggcaatg attaagatga gtccagagga aatcagagca aaatcgcaat
     1381 cttacgggca aggttcagac caaatccgtc aaattttatc tgatttaaca cgtgcacaag
     1441 gtgaaattgc agcgaactgg gaaggtcaag ctttcagccg tttcgaagag caattccaac
     1501 aacttagtcc taaagtagaa aaatttgcac aattattaga agaaattaaa caacaattga
     1561 atagcactgc tgatgccgtt caagagcaag accaacaact ttctaataat ttcggtttgc
     1621 aataagcatt ctgaaattgg caaagtcaca ttttctaatg tggctttgct tatcattttt
     1681 taagaaaaca actgaaagga aataagcatg aaaaagaaaa attggattta tgcattaatt
     1741 gtcactttaa ttattataat tgccatagtt agtatgatat tttttgttca aacaaaatat
     1801 ggagatcaat cagaaaaagg atcccaaagt gtaagtaata aaaataataa aatacatatc
     1861 gcaattgtta acgaggatca accaacgaca tataacggta aaaaggttga gctgggtcaa
     1921 gcatttatta aaaggttagc aaatgagaaa aactataaat ttgaaacagt aacaagaaac
     1981 gttgctgagt ctggtttgaa aaatggcgga taccaagtca tgattgttat cccagaaaac
     2041 ttttcaaaat tggcaatgca attagacgct aaaacaccat cgaaaatatc actacagtat
     2101 aaaacagctg taggacaaaa agaagaagta gctaaaaaca cagaaaaagt tgtaagtaat
     2161 gtacttaacg actttaacaa aaacttggtc gaaatttatt taacaagcat cattgataat
     2221 ttacataatg cacaaaaaaa tgttggcgct attatgacgc gtgaacatgg tgtgaatagt
     2281 aaattctcga attacttatt aaatccaatt aacgacttcc cggaattatt tacagatacg
     2341 cttgtaaatt cgatttctgc aaacaaagat attacaaaat ggttccaaac atacaataaa
     2401 tcattactga gtgcgaattc agatacattc agagtgaaca cagattataa tgtttcgact
     2461 ttaattgaaa aacaaaattc attatttgac gaacacaata cagcgatgga taaaatgtta
     2521 caagattata aatcgcaaaa agatagcgtg gaacttgata actatatcaa tgcattaaaa
     2581 cagatggaca gccaaattga tcaacaatca agtatgcaag atacaggtaa agaagaatat
     2641 aaacaaactg ttaaagaaaa cttagataaa ttaagagaaa tcattcaatc acaagagtca
     2701 ccattttcaa aaggtatgat tgaagactat cgtaagcaat taacagaatc actccaagat
     2761 gagcttgcaa acaacaaaga cttacaagat gcgctaaata gcattaaaat gaacaatgct
     2821 caattcgctg aaaacttaga gaaacaactt catgatgata ttgtcaaaga acctgattca
     2881 gatacaacat ttatctataa catgtctaaa caagacttta tagctgcagg tttaaatgag
     2941 gatgaagcta ataaatacga agcaattgtc aaagaagcaa aacgttataa aaacgaatat
     3001 aatttgaaaa aaccgttagc agaacacatt aatttaacag attacgataa ccaagttgcg
     3061 caagacacaa gtagtttgat taatgatggt gtgaaagtgc aacgtactga aacgattaaa
     3121 agtaatgata ttaatcaatt aactgttgca acagatcctc attttaattt tgaaggcgac
     3181 attaaaatta atggtaaaaa atatgacatt aaggatcaaa gtgttcaact cgatacatct
     3241 aacaaggaat ataaagttga agtcaatggc gttgctaaat tgaaaaagga tgctgagaaa
     3301 gatttcttaa aagataaaac aatgcattta caattgttat ttggacaagc aaatcgtcaa
     3361 gatgaaccaa atgataagaa agcaacgagt gttgtggatg taacattgaa tcataacctt
     3421 gatggtcgct tatcgaaaga tgcattaagc cagcaattga gtgcattatc taggtttgat
     3481 gcgcattata aaatgtacac agatacaaaa ggcagagaag ataaaccatt cgacaacaaa
     3541 cgtttaattg atatgatggt tgaccaagtt atcaatgaca tggaaagttt caaagacgat
     3601 aaagtagctg tgttacatca aattgattca atggaagaaa actcagacaa actgattgat
     3661 gacattttaa ataacaaaaa gaatacaaca aaaaataaag aagatatttc taagctgatt
     3721 gatcagttag aaaacgttaa aaagactttt gctgaagagc cacaagaacc aaaaattgat
     3781 aaaggcaaaa atgatgaatt taatacgatg tcttcaaatt tagataaaga aattagtaga
     3841 atttctgaga aaagtacgca attgctatca gatacacaag aatcaaaaac aattgcagat
     3901 tcagttagtg gacaattaaa tcaattagat aataatgtga ataaactaca tgcgacaggt
     3961 cgagcattag gcgtaagagc gaatgatttg aaccgtcaaa tggctaaaaa cgataaagat
     4021 aatgagttat tcgctaaaga gtttaaaaaa gtattacaaa attctaaaga tggcgacaga
     4081 caaaaccaag cattaaaagc atttatgagt aatccggttc aaaagaaaaa cttagaaaat
     4141 gttttagcta ataatggtaa tacagacgtg atttcaccga cattattcgt attattgatg
     4201 tatttactat caatgattac agcatatatt ttctatagct atgaacgtgc taaaggacaa
     4261 atgaatttca tcaaagatga ttatagtagt aaaaacaatc tttggaataa tgcgattacg
     4321 tctggtgtta ttggtgcaac tggtttagta gaaggattaa ttgtcggttt aattgcaatg
     4381 aataagttcc atgtattagc tggctataga gcgaaattca tcttaatggt gattttaact
     4441 atgatggtct tcgtacttat taatacgtat ttactaagac aggtaaaatc tatcggtatg
     4501 ttcttaatga ttgctgcatt gggtctatac tttgtagcta tgaataattt gaaagcggct
     4561 ggacaaggtg tgactaataa aatttcacca ttatcttata tcgataacat gttcttcaat
     4621 tatttaaatg cagagcatcc tataggcttg gcgctagtaa tattaacagt acttgtgatt
     4681 attggctttg tactgaacat gtttataaaa cactttaaga aagagagatt aatctaatgt
     4741 tgatgaatag cgtgattgct ttaacttttt taacagcatc tagcaataat ggcggactta
     4801 atattgacgt gcaacaagaa gaggaaaagc gaatcaataa tgatttaaat caatatgata
     4861 caacgctatt taataaagac agcaaagcgg ttaatgatgc gattgttaag cagaaaaaag
     4921 aacgacaaca acaaataaaa aatgatatgt ttcaaaatca agcgagtcac tcgactcgct
     4981 tgaatgaaac taaaaaagtg ttattttcca aatctaactt agaaaagact tcggagagtg
     5041 ataaaagccc ctatattcaa aacaagcagg agaaaaaaat attcccgtac attttgatgt
     5101 ctgtaggggc ttttttgact ttaggatttg tcattttttc aattcataaa gggagacgaa
     5161 cgaaaaatga atcagcacgt aaaagtaaca tttgatttta ctaattataa ttacggcaca
     5221 tatgacttag cagtaccagc atatttaccg ataaaaaatt taatagcttt agtattggat
     5281 agtttggaca tttcaatatt tgatgtcaat acacaaatta aagtgatgac gaaaggtcaa
     5341 ttacttgttg aaaacgaccg acttattgat tatcaaatcg ctgatggaga tattttgaag
     5401 ttactatagg aggaaaaata gatggttaaa aatcatgacc ctaaaaatga aatgcaagat
     5461 atgttaacac ctttagatgc tgaagaagca gctaaaacaa aattacgctt agatatgaga
     5521 gagattccta agtcttcaat taaaccagaa cattttcatt taatgtactt attagaacaa
     5581 cattctccat attttataga tgctgaatta actgaactac gtgatagttt ccaaatacat
     5641 tatgacatta atgacaatca tacacctttt gataatatta aatcatttac taaaaatgaa
     5701 aaattacgtt acttactcaa tatcaaaaat ttagaagaag taaatcgtac acgttacaca
     5761 tttgtgttgg caccagatga attatttttc acaagagatg gattaccaat tgctaaaaca
     5821 agaggtttac aaaatgttgt tgatccatta ccggtgtcag aagctgaatt tttaacaaga
     5881 tataaagcac tggttatctg tgcattcaat gagaaacaat catttgatgc tttagttgaa
     5941 ggaaacttag aactacataa aggaacgcca tttgaaacta aagttattga agcggcaacg
     6001 ttagatttac taacggcatt tttagatgaa cagtatcaga aacaagaaca agattatagt
     6061 caaaattatg catatgtacg caaagtagga cataccgttt tcaaatgggt tgctatcggt
     6121 atgacaacgt taagtgtttt attaattgca tttttagcct ttttatattt ttcagtaatg
     6181 aagcataatg agcgtattga aaaaggatac caagcatttg taaaggaaga ttatacgcaa
     6241 gtactaaata cgtatgatga tttagatggt aaaaaattag ataaagaagc actttacatt
     6301 tatgccaaaa gttatatcca aacaaataaa caaggtttag aaaaagataa gaaagaaaat
     6361 ttacttaata acgtgacacc aaattcaaac aaagactact tattatattg gatggaatta
     6421 ggacaaggac atcttgatga agcgattaat attgccactt atttagatga taacgatatt
     6481 acaaagttag cgttgattaa taagttaaat gagattaaaa ataacggaga tttatcgaat
     6541 gataaacgtt ctgaagaaac gaaaaagtat aacgataaat tgcaagatat tttagacaaa
     6601 gaaaaacaag ttaaagatga aaaagcgaaa tctgaagaag agaaagcaaa agcgaaagat
     6661 gagaaattaa agcaacaaga agagaacgaa aagaaacaaa aagaacaagc acaaaaagat
     6721 aaagaaaaac gccaagaagc tgaaagaaaa aaatagtata ggactgaggc aaagacaatg
     6781 cataaattga ttataaaata taacaaacaa ttgaagatgc tcaatttgcg agatggtaag
     6841 acatatacta ttagcgaaga cgagcgtgca gatattacgt tgaaatcgtt aggcgaagtc
     6901 attcatttag aacaaaataa tcaaggtact tggcaagcga atcatacttc tattaataag
     6961 gtgcttgtta gaaaaggtga ccttgatgac attacattac agctttatac agaagctgat
     7021 tatgcatcat ttgcgtaccc ttcgattcaa gatacgatga cgattggatc aaatgcatat
     7081 gatgacatgg tcattcaaag tttgatgaat gccatcatta ttaaagattt tcaatcgata
     7141 caagaaactc aatatgtgcg catcgtgcac gataaaaata cagatgtgta tattaactat
     7201 gaactgcaag agcaattaac gaacaaagct tacattggtg atcatattta tgttgaaggg
     7261 atatggctcg aagtacaagc tgatggttta aatgtattga gtcagaatac agtggcatcg
     7321 tcattaattc gcttaacaca agagatgcca catgcacagg cagatgatta caatacgtac
     7381 catcgttcgc caaggattat tcaccgtgaa ccgaccgatg atattaagat tgaaagacca
     7441 ccacaaccaa tacagaagaa caatacagtg atatggcgtt ccattatacc gccattagta
     7501 atgattgctt taactgttgt catcttttta gtgagaccaa ttggtattta tattttaatg
     7561 atgattggta tgagtacagt aacgatagta tttggtatta caacgtattt ctctgaaaag
     7621 aaaaagtata acaaagatgt tgaaaaacga gagaaagatt acaaagctta tttggataat
     7681 aaatctaaag agattaataa agcgattaaa gcacaacgtt ttagtttgaa ttatcattat
     7741 ccaacggttg ctgaaattaa agatatcgtt gaaacgaaag caccaagaat atatgaaaaa
     7801 acatcgcatc atcacgattt cttacattat aagttaggta ttgcgaatgt agaaaagtca
     7861 ttcaaattag attaccaaga agaagaattt aaccaacgtc gtgatgaact attcgacgat
     7921 gctaaagaat tgtatgaatt ttacacagat gtagaacaag caccattaat caatgattta
     7981 aatcatggac caattgcata tattggtgca cgacatctca ttttagaaga attggagaaa
     8041 atgctaatcc aattgtcaac gttccatagt tatcatgatt tagagtttct atttgtgaca
     8101 cgtgaagatg aagttgaaac attgaaatgg gcacgttggt tgccacatat gacattgaga
     8161 ggtcaaaaca ttagagggtt tgtttacaat caacgaacac gtgaccaaat tttaacgtca
     8221 atttatagca tgattaaaga acgtatccaa gctgtgcgtg aacgcagcag aagtaatgag
     8281 caaattattt tcacaccgca attagtgttt gtcattacag atatgtcatt aattattgat
     8341 catgtcattt tagaatatgt aaaccaagat ttatcagaat atggtatttc attaatcttt
     8401 gttgaagatg tgattgaaag tttgccagag catgtagata ccattattga tatcaagtct
     8461 cgtactgaag gcgaactgat tacgaaagaa aaagaattag ttcaattgaa atttacacct
     8521 gaaaatattg ataacgttga taaagaatat atcgcgagac gtttggcgaa tttgatacac
     8581 gtcgaacatt tgaaaaatgc aattcctgat agtattacat ttttagagat gtataacgtg
     8641 aaagaagtag atcagcttga tgtggttaat cgatggagac aaaacgaaac atacaaaacg
     8701 atggcagtac ctttaggtgt aagaggtaaa gatgacattt tatcattgaa cttacatgaa
     8761 aaagcacacg ggccacatgg tttagttgct ggtaccactg gttcagggaa atctgagatt
     8821 attcaatcat acattttatc tttagctatt aattttcacc ctcatgaagt tgcattccta
     8881 ttgattgact ataaaggtgg gggtatggcg aacttattta aagatttagt ccatttagtt
     8941 ggtacgatta caaacttaga tggcgatgaa gcgatgcgtg ccttaacatc aatcaaagcc
     9001 gaattgagaa aacgtcaacg tttattcgga gagcatgatg ttaaccatat taatcaatac
     9061 cataagttat ttaaagaagg tgttgcgaca gaaccaatgc cacatttatt cattatttcc
     9121 gatgagtttg cagaattaaa atcagaacaa cctgatttta tgaaagaact tgtatcaacg
     9181 gcacgtattg gacgttcgtt aggtattcat ttaatacttg cgacacaaaa accatcgggt
     9241 gttgttgatg accaaatttg gtctaactct aaatttaaat tggcattaaa agtacaagat
     9301 agacaagaca gtaatgaaat tttaaaaaca ccagatgcag cagacattac attaccaggt
     9361 cgtgcgtatt tacaagttgg taataatgaa atttatgaat tattccaatc tgcatggagt
     9421 ggtgcaacat atgacattga aggcgataaa ttagaagttg aagataagac gatttacatg
     9481 attaatgact atggtcaact tcaagcaatc aacaaagact tgagtggact tgaagatgaa
     9541 gaaacaaaag aaaatcaaac tgagttagaa gcggttatcg atcatatcga atctattaca
     9601 acacgattag aaattgaaga agttaagcgt ccatggttac caccattacc agaaaatgta
     9661 tatcaagaag atttagtaga aacagatttc agaaaattat ggtcagatga tgcaaaagaa
     9721 gtggaattaa cattaggact taaagacgta ccagaagaac aatatcaagg accgatggta
     9781 ttgcaattga aaaaagctgg gcacatcgcg ttaatcggaa gtccaggata tggtagaaca
     9841 acgttcttac acaacattat tttcgatgtt gcaagacacc atcgtcctga tcaagcacat
     9901 atgtacttgt tcgatttcgg taccaatggt ttgatgccag tcacagatat accacatgtc
     9961 gctgattact ttacagtaga tcaagaagac aagattgcga aggcgatacg gaagatacat
    10021 gatattattt ctgagcgaaa aagactatta agtcaagaga gagtagttaa tatagagcaa
    10081 tataataaag aaactggaaa ttcaattcca aatgttttct taattatcga taactacgac
    10141 acggtgaaag aatcaccatt tatggaagaa tatgaagaaa tgatgtctaa ggtaacacgt
    10201 gaaggtttag cgttaggggt atacatcatt ctttcaggat caagatcaag tgcaataaaa
    10261 tcagctatat ttactaacat taaaacaaga gttgcgctgt acctctttga aaacaatgaa
    10321 ttaacaaata ttattggttc ttataaaaaa ggtgtgaaag atgttaaagg tagagctgca
    10381 ataaatgatg ataacttcac acagttccaa attgcgcaac catttgaatt agcagaagga
    10441 caaacatata acgagcgcat taaaaatgaa gtagcacaaa tgaaagaatt ctatgtagga
    10501 gattatccaa aacatattcc aatgatgcca gataaggtat ttatggaaga tattcgagaa
    10561 gcatatgatt tggaaaaaat cattcatgaa gaacacaaat taccattagg attagacttt
    10621 gaggatgtgg aactagttag tttagatctg acaagttctt cgattgtaac agcaattaaa
    10681 ccaactgaga tggaaaaaat gaatgatgtt ataatgtcta gtttatcagt atatagtaaa
    10741 aaccaatttg ttatcttggt tgatgcagaa gataatatga gccagtattc agaagatgta
    10801 acctcttatt attcagcacc atcagatttg agcaacatta gattaggatt taagcaagaa
    10861 atagaagcaa ggaaaaatgg cgagaaaagt atagaggaat gcaagattgt atttataaat
    10921 aatattaaac gattcaatca attaactgga atgacagaag atgaaattcg tgtgttattt
    10981 aatgaaggtc aaaaagttaa tattatcatt attgcaagtg gactatactc agatacaatt
    11041 ggtgcatttg atagagaaag taaaatgatg gtaagaacta taaatcaagc tttgattagt
    11101 cataagatat cggaacaaga atttataaga gttaaagatc gatttggtga accagaatta
    11161 aaagtgggag aaatgtatta cattaataat caagaatatc aaaaaataaa actaatggag
    11221 ggatagagtt gataaagtta aaccaagcct ctgtcagcaa agaaatttca agcattcgaa
    11281 caaatggaca aggcttaaaa caatcaaatg ggaatgtaaa tttaagtaaa acgaatctcg
    11341 ttacttttaa agaatatgta aatatgtttg aagattatca atcagctcta tctaattatg
    11401 aaaacataat tgaacaagat acgacggcta tggatacaac ggtaacagaa attgttgaaa
    11461 acgatagaga aatagcagga caaattaata aataattaaa caagacgggt gagaaaaatg
    11521 agtaataagg gagaaataag aaggcaaatt gctaataaag aaagagaaaa agcaagtaaa
    11581 gaagcacaac taacagattt aaaagaggat ttaagaaggt tgaaagacgc ttcgaaaaaa
    11641 ttggatacag caggagaaga ttttaacaaa ggacagagta gttataacaa agtagaaatt
    11701 agtacttcag attggaaagg agaaagaagg actaaaagtg acagcaaaaa gaaagatgtc
    11761 gattctgaat taaaaaaagt tgaacaagac tttgatgatg cgaagaaggc tattaaaaaa
    11821 gatattcagg ataaagaaga agaaatcaag ggagtcgaag gagaaatcag tactataaat
    11881 gcagctatag atgcacttaa atcaaaatta taataataga gagggaaatt tattatgggg
    11941 tacaaagttg atatgtctga agtgcacaat atgcaaaagt ctattgattc aagcttaagt
    12001 gcaataaata caaaggttag cacgcttagc agtagcataa acaaccttat aaatactgaa
    12061 ggattcgaag gtcagacagc aagtagtgtc aaaaactata gtaagacatt tcatttacaa
    12121 tcaataaaaa agattgaaac aataaacaaa gattttaaat cagatattgc gaaatctata
    12181 cgtaaattcc aaagtgaagt tgataataat gcttcagcta tattggatga agacgaaata
    12241 aaaaaataca aaaaagatat tgatgatgca ttaaaagatg ttttcaaatc ttctaaagat
    12301 gcaaacggag ctatttcaga tgtttcagat ttaacaacag ccaaaaaaat aaagacagaa
    12361 aacttggcca ataaaatggg agattttaat aaagatattg atcaaacggt ggaaagatta
    12421 acgaccttcg acgccaacaa ttctatagat ggtgataaga cagataactt aataactgaa
    12481 ttaagtggtg taaattcata tgtcaaaaac atgaagccaa accgtgcaag aattagttct
    12541 acaagtggaa aaattgaagg tgcaattgca aggcataaaa caagtgaaga gttagtaaga
    12601 tggcaaactt acatggaaac gttaagtgaa aatctttata aaacaccagg tctaagtaaa
    12661 gcagcttctg acataattac ccaagctgga cgcgagtact atgctatcaa agctgtaggg
    12721 aatggcagtg ctttaagagg atataaagaa tatctgaaaa cacgagatat taataaatta
    12781 attaacaaaa tgaataaaaa ccaattaaaa agaatgtcat tggtattaaa tacagataga
    12841 ggtaatatta aaataaaaaa tgcagtaaaa atggcaagtg aatttgcccg taataatcct
    12901 tttaaaaaag gaaatttagt aaattggatg agcaaagtcc aagggtatca aagtgtaagt
    12961 gctcaaatat taaaaaaggg gttgcaagat aagaacttta aatatacatt tggagatgca
    13021 aagaaatttt ttgatacagc tgatatgaaa aaagcagcag tcacagaatt taaaaatacc
    13081 tttgtaccac aaaatataag agaaaccttc tttaagaaag aaaatttaac atctaagaca
    13141 gcattaaaga aaaatatgag taaattctgg aatgaagata ttaagggtgg aatcaaagag
    13201 tttaaattag actttgctaa caaaaatatc attggtaaac tcggcaaatt gttgaaacta
    13261 ggaggcaagg ctttaaaacc attaggattt attactgcaa ttactgacaa cataggaaag
    13321 aaatcttggc aagaaaaatt agttggtatg ggggtagatt taagtgctat tggtgcaagt
    13381 gcagctgcag gtgcagctgt tggaacggca ataccattac ctgtagtagg aacagttgca
    13441 ggtgcattag ctggagcagc agtgggtata gctttagatt ataaaatacc gtatcttgat
    13501 aagtctgcaa cagaattagc taaagatggt attaactcgg gtattaacaa agttaaaagt
    13561 ggatttggaa aagtaacttc aggatttaaa acagttttcg gataagaaag gggcagacaa
    13621 atgaaaatac aagagattgt aggaaatata aagagagctt atctgacacc attaataatt
    13681 tttgtgattt tgttttcatt attttttgat ttgataatgt atttttatgg aaaccttcaa
    13741 gggaaattgc caatatattt agatgtattt ctaatatttg cagcagttgt atttatttta
    13801 atgtacttcc aagagaaaag tggagaagtt aaggttgaaa aatcaaacgt tattcgttat
    13861 ttaacattaa atgttgtagc tgggtattca atggtactac ttgttgcctc tatttatgtt
    13921 tttggggtag caggctatgg ttttgatgta tttaattact ggttaggtat tatattaatg
    13981 ctatttgtat cgtggtttgc tctatttttg ttttataaaa atgaatttga tagtgaaaat
    14041 ccaaataaaa cagttaatgt tatagcaatt attataaagt tatctgcttt tggaggactt
    14101 ttctatatca gaacagtcgt tcctaatact gcagatgaag agaaatttat aactctaagt
    14161 atcttaatta atatagctgt tgatttattg ctggttagat catattttaa ctatgcatta
    14221 tataagagtg taaaaaaaga tatcgaaaac ggagtggatg attaatggaa gtaggaatgt
    14281 taattatgcc gttttgtgtg aagattacat ttgaaattga taaaaatgat aattggttaa
    14341 atgcattaga tacagtaaat gaatttttca tggacaaaga tatatatcca actggtccta
    14401 taattttcca aagagaatta gcaggtttgg
//
LOCUS       DATTQF010000059        14283 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.059, whole genome shotgun sequence.
ACCESSION   DATTQF010000059 DATTQF010000000
VERSION     DATTQF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.059"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..354)
                     /locus_tag="VB241_001469"
     CDS             complement(<1..354)
                     /locus_tag="VB241_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1244766.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKVGELLEFKNGLNKGKEYFG
                     SGSSIVNFKDVFNNRSLNTNNLTGKVNVNSKELKNYSVEKGDVFFTRTSEVIGEIGYP
                     SVILNDPENTVFSGFV"
     gene            complement(347..1903)
                     /locus_tag="VB241_001470"
     CDS             complement(347..1903)
                     /locus_tag="VB241_001470"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP1244767.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKEAKVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            1963..2082
                     /locus_tag="VB241_001471"
     CDS             1963..2082
                     /locus_tag="VB241_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244768.1"
                     /translation="MWWCRDVENFDVDGGNFIFMEIGIGFITRKQKRLNDDID"
     gene            complement(2165..2848)
                     /locus_tag="VB241_001472"
     CDS             complement(2165..2848)
                     /locus_tag="VB241_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HEP1244769.1"
                     /translation="MKLTALAKVTLALGILTTGTLTTEAHSGHAKQNQKSVNKHDKEA
                     LHRYYTGNFKEMKNINALRHGKNNLRFKYRGMKTQVLLPGDEYRKYQQRRHTGLDVFF
                     VQERRDKHDISYTVGGVTKTNKTSGFVSTPRLNVTKEKGEDAFVKGYPYDIKKEEISL
                     KELDFKLRKHLIEKYGLYKTLSKDGRIKISLKDGSFYNLDLRTKLKFKHMGEVIDSKQ
                     IKDIEVNLK"
     gene            complement(3211..3909)
                     /locus_tag="VB241_001473"
     CDS             complement(3211..3909)
                     /locus_tag="VB241_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HEP1244770.1"
                     /translation="MKLTAIAKATLALGILTTGVMTAESQTVNAKVKLDETQRKYYIN
                     MLKDYYSQESYESTNISVKSEDYYGSNVLNFNQRNKNFKVFLIGDDRNKYKELTHGRD
                     VFAVPELIDTKGGIYSVGGITKKNVRSVFGYVSHPGLQVKKVDPKDGFSIKELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKHEIDLSEKLSFDRMFDV
                     LDSKQIKNIEVNLN"
     gene            complement(4250..4945)
                     /locus_tag="VB241_001474"
     CDS             complement(4250..4945)
                     /locus_tag="VB241_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HEP1244771.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAAEKQERVQHLHDIR
                     DLHRYYSSESFEYSNVSGKVENYNGSNVVRFNPKDQNHQLFLLGKDKEQYKEGLQGQN
                     VFVVQELIDPNGRLSTVGGVTKKNNKTSETNTPLFVNKVNGEDLDASIDSFLIQKEEI
                     SLKELDFKIRQQLVNNYGLYKGTSKYGKIIINLKDENKVEIDLGDKLQFERMGDVLNS
                     KDIRGISVTINQI"
     gene            complement(5392..6096)
                     /locus_tag="VB241_001475"
     CDS             complement(5392..6096)
                     /locus_tag="VB241_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HEP1244772.1"
                     /translation="MKMTAIAKASLALSILATGVITSTAQTVNASEHESKYENVTKDI
                     FDLRDYYSRASKELKNVTGYRYSKGGKHYLIFDKNRKFTRIQIFGKDIERIKKRKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNQGAYYDYLSAPRFVIKKEVGAGVSVHVKRYYI
                     YKEEISLKELDFKLRQYLIQDFDLYKKFPKASKIKVTMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(6457..7371)
                     /locus_tag="VB241_001476"
     CDS             complement(6457..7371)
                     /locus_tag="VB241_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HEP1244773.1"
                     /translation="MKMTQIAKTSLALSLLTTGASIVTTQSANAEVASALKTEQAIKS
                     KIQKVTTSPSTKVKATQPTPSVTTPPSPNVQPQSPQPTPNPTTPHSSNVETKQPQSPT
                     TKQAQKEINPKYKDLRTYYTKPSLEFEKQFGFMLKPWTTVRFMNVIPDWFIYKIALVG
                     KDDKKYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSITKKDEKG
                     KISHDDSEYKITKEEISLKELDFKLRKQLIEQHNLYGNIGSGTIVIKTKNGGKYTFEL
                     HKKLQEHRMADVIDGTSIERIEVNLKSS"
     gene            complement(7718..8749)
                     /locus_tag="VB241_001477"
     CDS             complement(7718..8749)
                     /locus_tag="VB241_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HEP1244774.1"
                     /translation="MKIRTIAKASIALGLVTTGASIVTTQSANAEVASALKAGQLAKI
                     NVSTSAITTTAQAVNAEQNHTANPEQAAKSNTENVSTSLSTQTEQTPKSNIQKATQPA
                     PSGTTQSLPNAQPQSTQPTPSVTTPPSSNVETPQPTSPTTKQAQKEINPKFKGLRSYY
                     TKSSLEFKNELGIIIKKWTTIRFMNIVPDYFIYKIALVGKDDKKYGEGVHRHVDVFIV
                     LEQNKYGVDKYSVGGITKANRKKVDYKTGISITKEDKKGTISHDVSEYKITKEEISLK
                     ELDFKLRKQLIEQHNLYGNIGSGTIVIKMKNGGKYTFELHKKLQQHRMADVIDGTNID
                     RIEVNLKSS"
     gene            complement(9037..9738)
                     /locus_tag="VB241_001478"
     CDS             complement(9037..9738)
                     /locus_tag="VB241_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HEP1244775.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTVTEQPVHAENKHVQVSQNSKNL
                     KAYYTQPSVEYKNVTGYISSIQPKPGTKFLNMIEGNTVNNLALVGKDKEHYHTGVHRN
                     LDIFYVNEDKRFEGAKYSIGGITKASDKVVDQVAEARVIKEDHTGEYDYDFFPFKIDK
                     EAMTLKEVDFKIRKHLIDHYGLYGEMSSGTVTVQMKYYGKYTIELDKKLQEDRMADIV
                     KVIDIDRIEVKVKKA"
     gene            complement(10021..10701)
                     /locus_tag="VB241_001479"
     CDS             complement(10021..10701)
                     /locus_tag="VB241_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HEP1244776.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNIQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGVTKTNRGVYYDYIHSPILEIKKGNEEPQNSLYQIYKENISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            11173..12018
                     /locus_tag="VB241_001480"
     CDS             11173..12018
                     /locus_tag="VB241_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1244777.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVSVRQLDYFNQESMVKAFKGIDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNEFQQLVNDQPQTLQSF
                     LQENI"
     gene            12037..12396
                     /locus_tag="VB241_001481"
     CDS             12037..12396
                     /locus_tag="VB241_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEP1244778.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSRKTSETFNISVDKLK
                     DKNINVLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            12918..13070
                     /locus_tag="VB241_001482"
     CDS             12918..13070
                     /locus_tag="VB241_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244779.1"
                     /translation="MVPEEKGSITLSKDAATIFATAKFKPFQNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            13328..13654
                     /locus_tag="VB241_001483"
     CDS             13328..13654
                     /locus_tag="VB241_001483"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1244780.1"
                     /translation="MTTRERRSFSSKFKLQKVRLYENGKPKNEIIREYDLTTSTFSNP
                     IKQHQNTGSFNHQDNLKSDEKELIKLRKEVQHLKMENDVLKQILLITRRNRNHLTECV
                     SIFNIH"
BASE COUNT         4252 a         2536 c         2071 g         5424 t
ORIGIN      
        1 tacaaatcca ctaaacacag tattttcagg gtcatttaaa attacagacg gataacctat
       61 ttcaccaatt acctcactag tccttgtaaa aaaaacatca cccttttcaa cagaataatt
      121 ttttagttct ttgctattca cattaacttt tccagtcaga ttatttgtat ttaagctcct
      181 gttattaaat acatctttga agttaacaat cgacgatcct gagccaaaat attcttttcc
      241 tttatttaaa ccatttttaa attctaataa ctcgccaacc ttcttctctt cccattcgcc
      301 ttcaaaccct gggaatctca actctggcac atttttcgtt tgtgtattac tcatctttca
      361 acaccccaag ttctttcagg tatgcattga tttcttgttc aatttctgcg atttctttgt
      421 cgatattttt caaatcttgt tggacttgat ctaaatcaat tggcgcttct tcttcgaatg
      481 tatcgacata tctcggtatg tttaagttgt aatcgttatc ggcgatctct tgtaatgtcg
      541 cgctgtagct atacttatca attgtttcct tacgcttata tgtgtctata attcgttcga
      601 cttgggcatc gcttaaatgg ttttggtttt ttcctttttc aaaatcattg gatgcatcga
      661 taaataatac gttgtcgtct tgttggcgac attttttaaa tactaagata catgttggaa
      721 tacttgtccc atagaaaatg ttcgctggca aaccaatcac ggcttctaag tagttctttt
      781 cttcaattaa ataacgacga atgatacctt ctgcggcacc acggaataag acaccatgtg
      841 ggagtacaac ggccatggta ccttcatcat ctaggtaatg taccatgtgt tgaataaagg
      901 caaagtctgc tttagacttt ggcgcaagct tgccgtatcc gctgaagcgt tcgtcatttt
      961 caaattttga gtctgctgtc catttcgcgc tatatggtgg gttcgcaata accgcatcaa
     1021 atgtatgtcc taaaaaggct ggattttcca acgtatcatc attacggata tcgaaatttt
     1081 catatcgcac atcatgtaac aacatgttca tgcgcgctaa gttgtatgtg gtattgttac
     1141 gttcttgtcc aaaataacga tacacttttg cctctttacc aacgcgtaac agtaatgaac
     1201 ctgaaccaca tgttgggtca tatacatgac gtaatttatc tttaccgtct gtgacaatct
     1261 tcgccagtat cttagatact tgttgtggtg tatagaactc gcctgctttt ttacccgctg
     1321 tcgccgcaaa gcgcccgata agaaattcgt atgcatcacc taacatatca atttccatat
     1381 cactgtgaac gaatggtaag tcatcaaggt taaccataac tttggaaatt aacgcagtac
     1441 gttctttgac attgttacct aaacgcgttg aacttaagtc catatcgctg aacagtccga
     1501 taaagtcatt ttcactttct tcacctagtg ttgaagtttc aactttacga attgccgtcg
     1561 ccagatgttc gatatcgaaa tcttgcgttt caatttcacg aatcatcgca ctgaataaat
     1621 cttgtggctc aatgaagtaa ccaacttgat caattaattc tgcttttaag tcttcacggt
     1681 attcttcatc tgcccatgct tcttgatatg taatatcttc acctgccaag gcatctgcat
     1741 attcttgttc cgctttttca gataagaagc gatagaaaat caagcctaaa atgtaattac
     1801 ggaattcact cgcatccata ttccctctta aatcattcgc aattgaccat aattttttat
     1861 gtaattcagc ttgttgctga cgttgttttt cagtaataga catgtgattc ctccgccttt
     1921 gcataagtaa tttgtctctt tgtgtaatag atttattata acatttggtg gtgtcgcgat
     1981 gttgagaatt ttgatgttga tggtggaaat tttatattca tggagattgg gataggattc
     2041 ataacacgca aacaaaaacg gcttaacgac gatatcgatt gatgcatcct tttggtaagg
     2101 tatacgcacc aatttacttt tcatcgttaa gccgcttcaa ttacttttta ttattcataa
     2161 tgatttactt taaattcact tcaatatcct taatttgttt actatctatg acttccccca
     2221 tatgtttgaa ttttaattta gttcttaaat caaggttata aaagctacca tctttcagac
     2281 taattttaat cctaccatct tttgagagtg ttttataaag accgtatttt tcaattagat
     2341 gctttctcaa cttaaaatct aactctttca atgatatttc ttctttttta atatcataag
     2401 ggtaaccttt cacaaaagca tcttcaccct tttcttttgt aacatttaat cttggtgtgc
     2461 tgacaaagcc ggatgtttta ttggtctttg ttacaccacc aacagtatat gatatgtcgt
     2521 gcttgtctct tctttcttga acaaaaaaca catctaagcc cgtatgtctt cgctgttgat
     2581 atttacggta ctcatctcca ggcaataata cttgagtctt catccctcta tatttaaaac
     2641 gtaagttatt tttaccatgt ctcaaagcat taatgttttt catttcctta aagtttccag
     2701 tgtagtatcg gtgtaatgct tctttgtcat gtttgtttac tgacttttga ttttgtttag
     2761 catgacctga atgggcctct gttgttaaag ttcctgttgt taaaatccct aatgctaatg
     2821 ttacttttgc taatgctgtc aatttcatat ttatttactc caatcttaat gtattggatt
     2881 gttattatta cgtaatttga atcatatacg cttattataa cgtccatcgt ttaacgcgat
     2941 aattaacctc gatttaaata tgcgtgaatt attattaatt aaaaacaaat atattttaat
     3001 gtccaggtta attaactttc acgctttaca tgtcgttgtt tttccggatc atcgttttct
     3061 acaactaatt gtgttatttc tactctttga aacgcaaaaa acaacttaac aacaataatg
     3121 tatttaacac agttcttatt caaaggtacg ggtgtcaatg cacattttat cgttaagccg
     3181 ctttttaatt ttttatacta tcatctttaa ttaattcaaa ttcacttcaa tatttttaat
     3241 ctgcttacta tccagcacat caaacatacg atcaaaactt aatttttcac ttaaatcaat
     3301 ttcatgtttc ttttcgtctt tcatattgat aacaattcta cctttatctg acgcgccttt
     3361 atacaatcta tatttttcga ctaacatttt tctgatttta aaatcaagtt ctttcaatga
     3421 tacttcttct ttttgaataa agaacaactc ttttatcgaa aagccatctt taggatcaac
     3481 ttttttaact tgcagtcccg gatgacttac atagccaaac acggatctca catttttctt
     3541 tgttataccg ccaacgctat atatgccgcc tttagtatct attaattcag gtactgcaaa
     3601 gacatcacgg ccatgcgtca gttctttata tttatttcta tcgtcgccaa ttagaaatac
     3661 tttgaaattt ttatttcgtt ggttaaagtt taaaacgtta gacccataat aatcttcact
     3721 tttaacacta atgtttgttg attcatagct ttcttgagaa tagtaatctt ttagcatatt
     3781 tatataatat ttgcgttgtg tttcatccaa ctttactttc gcgtttacag tttgactttc
     3841 tgctgtcatc acacctgttg ttaatattcc taatgctaat gttgctttag ctattgctgt
     3901 taatttcata atttattgct ccaatcttaa tgtattggat tgttatgatt acgtaatttg
     3961 aaacatctgc ctttattata aagtgcatcg aaacaaactg acattaacgt gcgtttaaat
     4021 aactttgaat cattgttaat gaacacctaa ttaatattca aagtacgtgg ttttaatgag
     4081 tggtatgact tatttagtat actcgtgact tgtgttatca aatgtaaaaa acagcctaac
     4141 gacgacgttg ctttaaatac gctcctttga taggatatac atatttattc aacgtttcat
     4201 cgttaagctg cctcaaaatt tattacttta aagtcattga ttgcttactt taaatttgat
     4261 taatagtgac tgatatacct ctaatgtctt tactattcaa cacatcgccc atgcgctcga
     4321 attgtaattt atcacctaaa tcaatttcta ctttattttc gtctttcaaa ttgataatga
     4381 ttttaccgta tttagatgta cctttatata atccgtaatt attaactaat tgttgtctaa
     4441 ttttgaaatc aagctctttt aatgagattt cttctttttg gattaaaaat gagtcaattg
     4501 atgcatctaa atcttcacca ttaactttat taacaaataa aggtgtatta gtttcagaag
     4561 ttttgttgtt tttcttcgtt acaccaccaa cagtagatag tctgccgttt ggatcaatta
     4621 attcttgtac tacaaagaca ttttggcctt gtagaccttc tttatattgt tctttatctt
     4681 ttcctaataa gaataattgg tgattttgat cttttgggtt aaagcgtaca acgttagaac
     4741 cattgtagtt ttcaacctta ccactaacat tactatattc gaaactttct gatgagtagt
     4801 atcgatgtaa atctctaata tcatgtaaat gttgtactct ctcttgtttt tctgccgctt
     4861 gaactgcttg accttctgat gtaatgacac cagtagttaa taaacctaat gctaatgttg
     4921 ctttagctaa cgtttttaat ttcatagtat tattctccca atctattttt aaattttgac
     4981 ttaatatatg tttatgtgat taattcaatt acgtaatacg aacaatctac tatcattata
     5041 taagccggat actgcgctat gaattaaccg ctttttaact attcctgaac caatgttaag
     5101 ctaattaatg gaatcctaac agtattaatc attttttata actctatgct atttttttca
     5161 ccattcacac tttctttcaa tttaattatg ttttccacat tgtttcatgt cactagtgcg
     5221 acaccgtgcg actataggta tcaactattt tcacagtttt tattgtcgtt attatctttc
     5281 atcgacccac ttcatttccg atcgtaacga cacaaaaaag aagctaagca acctatgctg
     5341 cctagctcat ctatactatc catattttac tattatccat gtttcgttga attatctaat
     5401 attggcttct attttctcaa tatttctgcc atctatgacg tcactcattc ggtttgtttg
     5461 taacttttta ttgagttcaa acgtataata gccgccatct ttcattgtca cttttatctt
     5521 gctagcttta ggaaactttt tatacagatc aaaatcttga attaaatact gtcgtaattt
     5581 aaaatcaagt tctttcagcg aaatctcttc cttataaatg tagtatcttt taacatgcac
     5641 agaaacacct gcacctactt cttttttaat aacgaatcta ggtgcgctta agtaatcata
     5701 gtaagcgcct tgatttttct tagtaacgcc accatatgaa tacactgtgc cgttgcgatt
     5761 ttccgcttct ttaactacaa atatatctaa tcctggattt ttacgttttt taatcctttc
     5821 tatatcttta ccaaaaattt gtattcttgt gaactttcta tttttatcaa agataaggta
     5881 gtgcttgcca cctttgctat aacgatagcc agtaacattt ttaagttcct tacttgcgcg
     5941 actatagtaa tctctcaagt cgaagatatc ttttgtcaca ttttcatatt ttgattcatg
     6001 ttcgctcgca tttacagttt gagccgttga tgttataacc ccagtcgcta aaatacttag
     6061 agctaaactg gctttcgcaa ttgctgtcat cttcatagtt gtatgctcca atcgtaatta
     6121 ttagatttgt tcgattacgt ttattgaatc atacagcttt attatagttg gcgtatgtct
     6181 ctattcatat taaaccttgt ttaactatat ttgaatcatc gttaattaaa ttaaggaatc
     6241 cataatgttc gttaaataaa aatgactttt gttgtacttc aacgcttgat agattggtat
     6301 ttttagacac atttaggaca taagcatgtt atgtgtggtc aacatcaaat attcgtcttc
     6361 ttataccact gtgcaacacg aaagaagctg agcaacatgt aaatttttgt cactcaactt
     6421 ctcggtacat cgttcaacgg ttctatgcag tatccatcac gatgatttta gattcacttc
     6481 aattctttcg atactagtgc catctatgac atctgccatg cgatgttctt gtaatttttt
     6541 atgcaattca aacgtgtact tcccaccgtt tttcgtttta ataacaattg ttcctgaacc
     6601 aatattaccg tacaaattat gttgttcaat gagttgcttt ctcaatttaa aatcaagctc
     6661 tttcaaggaa atctcttctt tagttatctt atattctgaa tcatcgtgtg agattttgcc
     6721 tttctcatct tttttagtaa tgcttaattc tgctttgtga tcaacttttt tactatttgt
     6781 cttcgtgata cccccgacag aatatttttt caattgatat ttattgtcct ctaaaacgat
     6841 aaatacatca atattatcgt atggcccatc tttatatttc ttatcatctt ttccaactaa
     6901 agctatttta taaatgaacc aatctggtat aacattcata aacctaactg tcgtccatgg
     6961 tttcagcata aagccaaatt gcttttcgaa ttctaaactc ggtttcgtat aatatgttct
     7021 taaatcctta tatttagggt ttatttcctt ttgtgcttgt tttgtggttg gcgattgcgg
     7081 ttgttttgtt tcgacattag atgaatgcgg tgttgttggg tttggtgttg gttgtggcga
     7141 ttgcggttgt acatttggtg aaggcggagt tgttacgctt ggtgttggtt gtgttgcttt
     7201 tacttttgtt gaaggcgatg ttgttacttt ttgtatttta gattttattg cctgttctgt
     7261 tttaagtgct gatgctactt ctgcattagc cgattgcgtt gttactatac ttgcacctgt
     7321 tgttaaaagt gatagtgcta aactggtttt cgcaatttgt gtcattttca tagttgtatg
     7381 ctccaatcta ttatattcga ttgttcttta cgtaattaga atcatacaac tacattatag
     7441 aagacgtatc ggtctattca cattaaacca tgtttaacta tatttgaatc atcgttaagt
     7501 aatccataat tttcgttaaa tacagatggt ttcgaagcga ttcaacgctt gcacattggg
     7561 cattgcatca ttttcaagac attgagatgt cacttttaca aacaagtgaa taatttccct
     7621 taacatcatt acgcaaaaca aaagaagcta agcaatgatg taggtcattg tcgcttaact
     7681 tctcatttca tcgttcaacg gttctatgta gtatccatca cgatgatttt agattcactt
     7741 caattctatc aatatttgta ccatcaataa catctgccat gcgatgttgt tgcaattttt
     7801 tgtgtaattc aaacgtgtac tttccaccat ttttcatttt aataacgatt gttcctgaac
     7861 caatattacc gtacaaatta tgttgttcaa tgagttgttt tctcaattta aaatcaagtt
     7921 ctttcaagga aatctcttct ttagtaatct tgtattctga aacatcatgt gagattgtac
     7981 cttttttatc ttctttagta atacttattc cagttttgta atcaactttc tttctatttg
     8041 cttttgtgat accaccgacc gagtatttgt ctacgccata tttattttgt tctaaaacga
     8101 taaatacatc gacatgccta tgcacgcctt caccatattt cttatcatct tttccaacta
     8161 aagctatttt atatatgaaa taatctggaa caatattcat aaatcttatt gttgtccatt
     8221 ttttgataat tatacccaac tcatttttaa attctaaact tgatttcgta taatatgatc
     8281 ttaaaccttt aaatttaggg tttatttcct tttgtgcttg ttttgtggtt ggcgatgttg
     8341 gttgtggtgt ttcgacatta gatgaaggcg gtgttgttac acttggtgtt ggttgtgttg
     8401 attgcggttg tgcatttggt aaagactgag ttgttccgct tggtgctggt tgtgttgctt
     8461 tttgtatgtt agattttggc gtctgttctg tttgagttga aagcgatgtt gatacatttt
     8521 ctgtgttaga ctttgccgcc tgttcaggat tagccgtatg attttgttct gcgttcactg
     8581 cttgcgctgt cgttgtgatt gcgcttgttg atacgtttat ctttgctaac tgtcctgctt
     8641 taagtgctga tgctacttct gcattggccg attgcgttgt tactatactt gcacctgttg
     8701 ttacaagccc taacgctata ctcgctttcg caattgttct tattttcata gttgtatgct
     8761 ccaatcgtaa ttattcgatt gttctttacg taattagaat catacaactt cattatagag
     8821 gacatattgg tctattcaca ttaaccctcg tttaacaatt gttgaaatat tattaagtca
     8881 tttaagcaaa aaataatgag tactttcgag gtttatgtgg tgttactatg tatctttgaa
     8941 atgattttaa gacataaaaa agaagttaag taacgtttta tcatcactta acctcactat
     9001 tgcaatttca attcattacg acgaccagtc taagcattat gcttttttaa ctttgacttc
     9061 aatcctatca atatctatga ctttgacgat atcggccatt cggtcttctt gtaacttttt
     9121 atccaattca attgtatact tgccatagta tttcatttgg acagtaactg ttcctgaact
     9181 catttcacca taaagaccat aatgatcaat aagatgtttt cttattttaa aatcaacctc
     9241 ttttaatgtc attgcttctt tatctatttt aaatgggaaa aagtcataat catattcacc
     9301 agtgtgatct tctttaatga ctcttgcttc tgctacttgg tcgacaactt tatcgcttgc
     9361 ttttgtgata ccaccaatcg agtactttgc accttcaaat ctcttatcct cattaacata
     9421 aaatatatca aggttgcgat gtacacccgt atgatagtgt tccttatctt tgccaactaa
     9481 agctagatta ttaactgtat taccttctat catattcaaa aatttagtgc ctggtttagg
     9541 ttgaatacta ctgatataac ctgtcacatt tttatattca acactaggtt gagtatagta
     9601 cgcttttaaa tttttactat tttggcttac ttgaacatgt ttattttcgg catgcaccgg
     9661 ttgttccgtt acagtattta cacctgtagc taaaataccc aacactaaac ttacttttgc
     9721 aattgatttc attttcataa ttgtatgctc caatatatta gattagattg ttttattacg
     9781 taaattgaat catacaacca tattatagga gctgtatgct gatattcaca ttaacccttt
     9841 tttaactatt cataaaatat gattaagcta gttaagtaaa agataatgcg tgctgcgact
     9901 gtttgtgtga gttttgtaac tttgccctca aaaaagaagc taagcaacag tacgtcgctt
     9961 aacttccata tatttccata acaagcacga ttcaacatag cgctttgtcg ttagaaagta
    10021 ttatttcatt tctactagaa ttttttgtat ttgtctgccg tcgatagaat cacccatacg
    10081 ttctttttca agtttttgac ttaaatcgat agtatgtgat ttgccatctt tcatagtaat
    10141 tgtaatttga ccttgtttaa gaccatttga atacaagccg tgttgtttga ttgcacgttc
    10201 tcgtaatcta taatcaagtt cttttaatga gatattttct ttataaattt gatacagact
    10261 attttgtggt tcttcattac ctttcttaat ttcaaggatt ggagagtgta tatagtcgta
    10321 atacactcct ctattagttt ttgttacgcc accaatcgaa tgattatcag cttgtctacc
    10381 gtttccttct ctaacgacaa acacatctat atctgggtta acttcttcat tatatttatc
    10441 tttatcagaa ccaactaaag atattttatt atattgtcca ttgtatataa aatcaatgaa
    10501 tctatcttta ccttcaataa atccatatcc agttactttt ttatattcaa aacttggtcc
    10561 attataataa agttttaaat cagcctcact ttgttgttta acttctgttt tcgcttgtac
    10621 tgattgtata ttcgacgtta ttacacctgt tgctaacatt cccaatgcta aacttgcttt
    10681 tgctatcgct ttaaatttca taatttttag ccccaatcta tttaaatttg atttttcaat
    10741 tacgtaatat gaacaatcta cctacattat attgaggcgc tattcccttt caaattaaac
    10801 ctcatttaac taaacttgaa tcattgttaa ggtaattaat aaaatgctaa ctgttaataa
    10861 tcaattctta tccgtcaaga ctctttttta tacaattcac actttaaacc attttaattt
    10921 taaaaactgt aaaactttca gacacaaaaa tgacatcata tcgtcgcagt accatgtcaa
    10981 aattttcaaa gatcatggtg acacgcatca actaaaacgc gtcattacgg tatttttaac
    11041 attaataaca tatgcgtatt tactatacac ggcgacaata aatacagatt gcaatttttt
    11101 ataaaatcaa atgttttgaa aatcatcgaa taagtattat gttatgtata taaagattag
    11161 gagatgaagt gaatgaatat tatgttaaca ggtgctacag gtcatttagg cacacatatt
    11221 acaaatcaag ccattgcaaa tcacatagat cattttcaca ttggtgttag aaatgttgag
    11281 aaagttccag aagattggcg cggaaaagtt tctgttcgac aattagatta ttttaatcaa
    11341 gaaagcatgg taaaagcatt taaaggcatc gacaccgttg tgtttattcc aagtattatc
    11401 catccatcat ttaaacgtat tcctgaagtg gaaaatttag tatatgccgc aaagcaaagt
    11461 ggcgttgctc atatcatttt cataggttat tacgcagatc agcataataa tccattccat
    11521 atgagccctt atttcggata tgcatcacgt ttattggcaa caagtggcat tgattatacg
    11581 tatgtaagaa tggcgatgta catggatcca cttaaaccat atttaccaga attgatgaat
    11641 atgcataaac tgatttatcc agctggtgat ggtcgtatta attatattac tagaaatgat
    11701 attgctagag gtgtcattgc tattattaaa aatccagata cttggggcaa acgttattta
    11761 ttatcaggct acagttatga tatgaaggaa cttgctgcaa ttttatctga ggcatcaggc
    11821 acagaaatta aatatgaacc cgtttcatta gagacatttg cagaaatgta cgatgaacct
    11881 aaaggctttg gtgcattatt ggcatcaatg tacgacgcag gagcaagagg actattagac
    11941 caagaatcca atgaattcca acaattagtc aatgatcaac cacagacact gcaatcattt
    12001 ttacaagaaa atatttaata ctaaaggagc gttacagtga atatcatctc gacaatttta
    12061 atcatatttg tggcattaga atttttctat attatgtacc ttgaaacgat tgctacaact
    12121 tccagaaaga ccagcgagac atttaatata agcgtcgata aattgaaaga caaaaatatt
    12181 aacgtacttt tgaagaatca aggcgtatat aatggtttaa tcggagtttt gctaatatac
    12241 ggtttattta tcagcagtaa tccaaaagaa atatgcgcag ctattttagt gtatatcatt
    12301 ggcgttgcta tttatggtgg cctttcaagc aatattagta tctttttcaa acaaggcaca
    12361 ttgccagtat tggcacttat atcaatgctt tggtaagtat tggtgttttg gggtttggag
    12421 atgtagtcgg aggtttggag gatttgagcg atttgggtat ggactttaga ctcagagtat
    12481 ttcaacctaa ttatttcaag cagaggtgac agtagcgttg cctctgtttc cttatagaaa
    12541 aattatttaa tgaagaaaat tcatatctgt tttaattttt agttgataaa tactccatat
    12601 attagaatgg ctactttatc tattgcatca atcctttaaa acaaaaaacc catgatttcg
    12661 aaattcccgt atggtggggt tcctactctc atgggtcagt taactaaatt atcactatca
    12721 gtttataatt tcaatattat taacaatata tgtagtcgca aaaggaatga ggacatgagg
    12781 actttggtgt tgattggcat tacataacgc ttccaaacaa attattcggt aacaataaga
    12841 aactatttac acaaattatt atacatagta aaattatttt ataaacttta aatcaaattg
    12901 tacaaggagt gaatatcatg gtaccagaag aaaaaggttc tattactttg tcaaaagatg
    12961 cagctaccat atttgcaact gcaaaattca aaccatttca gaacagaatt aaaaataacc
    13021 cacaaaaaac aaatccattt cttaaattac atgaaaacaa aaaatcttaa tcactttatt
    13081 tatagcattt ctgatctcag aaatttaata aaggacaacg ccctctttca taactatata
    13141 agtcaagaaa ccaagaaatt ccaaacatct ttaaaccctt caatagcaca tttcctaaaa
    13201 cataaagcaa taacaatgaa cgatcaaaat tcctaacgta cttatgatgt gtcagtgaat
    13261 cgtctacact taattgaaca aattctatga gaatagatat tgttaattta agaaagtagg
    13321 ctattttatt acgacaagag aaagaagatc atttagttca aagtttaagt tacaaaaggt
    13381 tagattatat gaaaatggta agcctaaaaa tgaaattata cgcgagtatg atttaacaac
    13441 ctcaactttc tcaaatccga taaaacaaca ccaaaatact gggtccttca atcatcaaga
    13501 taatttaaaa agtgatgaaa aagagttaat aaaattacgc aaagaagttc aacatttaaa
    13561 aatggaaaat gatgttttaa agcaaatatt gctaatcacg agacgaaata gaaatcattt
    13621 aacagaatgc gtatcaatat tcaatatcca ttaatgtgca aagtcttaga agtacttatt
    13681 atgaatcaat aaaaaggaat aataagagca aaaaacatgc tgaattggat aatcgttcac
    13741 tcccttaaca aatatatgta cttttgaata acttgtaatg taaatctact tgactgatat
    13801 aaaccttcat ccactttatg aacaatttta tttactaaag ttttttatat ttacgataat
    13861 cttttacgtt tatcttctta ttcaacggta tctacaaaaa cactctatac gtttcaattg
    13921 tatgattgaa tgtattatag tgtgcgaaaa ctatgcaaca ctgctttaaa ctgatctgaa
    13981 cgcatcatgt atctgcttta aatcatttac ttcatcataa taaaatacaa ttacatatat
    14041 ataatatttt tataattgca gtgctttttg attaattcgc catgattatt ttcacagact
    14101 ttaatatcac catacacagc aacaccactg aaacatgtat atcgcatttt aatttaattt
    14161 catccatttt ttgtgcctct aatataacaa tttttgtgcg tcatttaatt cggtcgcaaa
    14221 acttatatca gaagatattt tttctatttc ttgaaacttt tgtataagtt catcaaattc
    14281 ttt
//
LOCUS       DATTQF010000060        14229 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.060, whole genome shotgun sequence.
ACCESSION   DATTQF010000060 DATTQF010000000
VERSION     DATTQF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.060"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            29..163
                     /locus_tag="VB241_001484"
     CDS             29..163
                     /locus_tag="VB241_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HEP1244781.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            287..982
                     /locus_tag="VB241_001485"
     CDS             287..982
                     /locus_tag="VB241_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEP1244782.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFYYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHNLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNEIVR"
     gene            complement(1090..1530)
                     /locus_tag="VB241_001486"
     CDS             complement(1090..1530)
                     /locus_tag="VB241_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1244783.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            1725..3338
                     /gene="bshC"
                     /locus_tag="VB241_001487"
     CDS             1725..3338
                     /gene="bshC"
                     /locus_tag="VB241_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEP1244784.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQISFEKRMS
                     KENNGREAALANVIREYMNDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQKRTQNQGLKAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFRLNKTDKKYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFIGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASDQFIEKVEGMIEQQRRLYQDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            3482..3913
                     /gene="mraZ"
                     /locus_tag="VB241_001488"
     CDS             3482..3913
                     /gene="mraZ"
                     /locus_tag="VB241_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEP1244785.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            3929..4864
                     /gene="rsmH"
                     /locus_tag="VB241_001489"
     CDS             3929..4864
                     /gene="rsmH"
                     /locus_tag="VB241_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP1244786.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            4878..5279
                     /gene="ftsL"
                     /locus_tag="VB241_001490"
     CDS             4878..5279
                     /gene="ftsL"
                     /locus_tag="VB241_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP1244787.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            5260..7494
                     /locus_tag="VB241_001491"
     CDS             5260..7494
                     /locus_tag="VB241_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1244788.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            7786..8751
                     /locus_tag="VB241_001492"
     CDS             7786..8751
                     /locus_tag="VB241_001492"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP1244789.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            8753..10102
                     /locus_tag="VB241_001493"
     CDS             8753..10102
                     /locus_tag="VB241_001493"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP1244790.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDKNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            10118..11437
                     /locus_tag="VB241_001494"
     CDS             10118..11437
                     /locus_tag="VB241_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEP1244791.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQGNEENNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQETDDQNESNGIAVDNEQHQSAPNEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKVRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLARDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSTQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            11543..12949
                     /gene="ftsA"
                     /locus_tag="VB241_001495"
     CDS             11543..12949
                     /gene="ftsA"
                     /locus_tag="VB241_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP1244792.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESSDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            12982..14154
                     /gene="ftsZ"
                     /locus_tag="VB241_001496"
     CDS             12982..14154
                     /gene="ftsZ"
                     /locus_tag="VB241_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP1244793.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         5271 a         1938 c         2729 g         4291 t
ORIGIN      
        1 caaatttaaa ttttaaggag tgttttcaat ggaaggttta tttaacgcaa ttaaagatac
       61 cgtaactgca gcaattaata atgacggcgc aaaattaggc acaagcattg tgagcatcgt
      121 tgaaaatggc gtaggtttac taggtaaatt attcggattc taatataata actaatattc
      181 tttaaaataa actgggtgag catactttaa tgttatgcac tcagtttatt ttatttgcag
      241 aaatttgagc ctctgttaag atttagatac atagacaata taggagatgg ggaaattggg
      301 atataaaaat attttgatag actttgatga tacaattgtt gatttttatg atgcagaaga
      361 atgggcattt tactatatgg cgaatgtttt taatcataaa gcaacaaagg atgatttttt
      421 aacatttaaa aaaatcaatc accaacattg ggaagctttt caacaaaata aattaacgaa
      481 gtctgaagta ttatcagaac gatttgtaaa ttacttcaaa catcatcaaa tggaagttga
      541 tgggcatcgt gcagatgtgt tatttagaaa tggattagca gaagctaaag ttaaatactt
      601 tgatcaaaca ttagaaacaa ttgtcgagtt atcgaaaaga cataatctat atattgttac
      661 taatggtgta acagaaacgc aaaagagaag gttaaatcag acgccattgc ataaatatat
      721 taaaaagata tttatatctg aggaaacagg atatcaaaaa cctaatccgg aattttttaa
      781 ttatgttttt aatgatattg gtgaggatga aagacagcac tcgattatag ttggagattc
      841 tttaacatct gacattctag gtggaatcaa tgcgggtata gctacttgct ggtttaattt
      901 tagaggattt gatcataatc caggaattat acctgattat gaaattaatt catggaaaca
      961 actaaatgaa attgtacgtt aagacaaata atttgaaaat gatttactta tattagctaa
     1021 gaaagtaatt gtaaaaaaga cactacgaaa agatgcattt tcattctcgt agtgtcgaat
     1081 attattatct tatggtttcc acaataagac atcatgtccc tctatatttt tagcttccac
     1141 atctgtaaat ccatgatgta aaaagaaatc cttagaatca tttctgccaa tggctttaat
     1201 tggcatattg aaactctttg caaaatcaat caattctgaa gcgtaccctc tgttttgata
     1261 ttttggtaat acttctaact tccataataa tatataatct tcaaaatctg ggaagtagat
     1321 ttcttcgaca tcaccttttt taagtaatgc cattctagct cctaattgat ctccgacaaa
     1381 tatgccataa aatggtgaat ctgaacttgc gtcaatcatt tgaccgttta actcattgac
     1441 catgtataag tctttgttgc caaacgctct aaagttttcg aataattcgt cagttttgta
     1501 attaatttca agacgtttga tttcactcat atttatctac cccttatttg tttactacta
     1561 ttattataca ttaaaccact tgtgtttttc attgatttga aaatgtaaaa caatgaatat
     1621 tataaatttt ccatgattaa attgattttc aagccgtaaa attgtcaatc accggtatag
     1681 actttatact atagtttgta atcgcgtact taaggagaaa aaacatggac tgtaaagtag
     1741 ttagtttaaa tgaaaaagat cagtttatac caaaaataaa gagcagtgat cctgtaataa
     1801 caggattatt tcaatatgat gcagctcaac aaattagttt tgaaaaaaga atgtctaaag
     1861 aaaataatgg aagagaagcg gcattagcga atgttattcg tgaatatatg aatgatttaa
     1921 agctttctag tgaacaagaa ttaaacatac aacatttagc taatggttca aaagttgtga
     1981 ttggtggaca acaagcaggg cttttcgggg gaccattgta tacattccat aaaatatttt
     2041 caatcattac cttatctaag gaattaacgg atacacataa gcaacaagta gtaccagttt
     2101 tttggattgc aggagaagat catgatttcg atgaagtgaa tcatacattt gtttataacg
     2161 aaaatcatgg gtcactgcat aaggttaaat atcatacaat ggagatgcca gagacgactg
     2221 tctctagata ttatcctgat aaggctgagt tgaaacaaac tttaaaaacg atgttcattc
     2281 atatgaaaga aactgttcat acacaaggtc tacttgagat ttgtgacaga attattgacc
     2341 aatatgactc gtggactgat atgtttaaag cactactgca tgaaacattt aaagcatatg
     2401 gcgttctatt tatagatgcg cagtttgagc cgttaagaaa aatggaagcg cctatgttta
     2461 aaaagatttt gaaaaaacat cagttgcttg atgatgcttt tagagcaaca caaaaacgta
     2521 ctcaaaatca aggcttgaag gcgatgatac aaacagatac aaatgttcat ttattcttac
     2581 atgatgaaaa tatgcgtcaa ttagtttcgt atgatggtaa gcattttaga ttaaataaaa
     2641 cagataagaa atatataaag gaagaaatta ttaatattgc ggaaaatcaa cctgaattat
     2701 tttctaataa tgtagtgaca agaccattaa tggaagaatg gctatttaac acggtggcat
     2761 ttattggagg gccgagtgaa attaagtact gggctgaact aaaagatgta tttgaactat
     2821 ttgatgttga aatgcctatc gtgatgccaa ggcttagaat tacttattta aatgaccgta
     2881 ttgaaaaatt actttcgaaa tacaatattc cattagaaaa agtgttagtc gatggtgttg
     2941 aaggagaaag aagtaagttt attagagaac aagcatcaga tcaatttatt gaaaaggtag
     3001 aaggtatgat agaacaacag cgtcgtctat accaagactt attagatgaa gtggcgggga
     3061 atcaaaataa tattaacctt gtgaataaaa ataatgaaat tcatatacaa cagtatgatt
     3121 atttgttaaa acgttatctt ttaaacattg aaagagaaaa cgacatcagt atgaagcaat
     3181 ttagagaaat tcaagaaaca ctccatccaa tgggaggatt acaagaaaga atatggaatc
     3241 cacttcaaat tttgaatgat tttgggacag atgtgttcaa gccctccacc tatccaccac
     3301 tttcttacac ttttgatcat attattataa aaccttaata taccaagggt ttagcccgat
     3361 ttatcataat gataaatcgg gcattttttt gttttttaaa ataaatttca caaattttgt
     3421 ataaatagtg gtggatagtg gggagatgtg gtaaattata tataaggtga ggtgataaaa
     3481 aatgttcatg ggagaatacg atcatcaatt agatacaaaa ggacgtatga ttataccgtc
     3541 caagtttcgt tatgacttaa atgagcgttt tattatcaca agaggccttg ataaatgttt
     3601 attcggttac actctagacg aatggcaaca gattgaagag aaaatgaaaa ccttacctat
     3661 gacaaaaaaa gacgcacgta agtttatgcg tatgttcttc tctggtgctg ttgaagtaga
     3721 acttgataag caagggcgta ttaacatccc tcaaaacttg aggaaatacg ctaatttaac
     3781 taaagaatgt acagtaatcg gtgtttcaaa tcgtattgag atttgggata gagaaacttg
     3841 gaatgatttc tatgaagaat ctgaagaaag tttcgaagat attgctgaag atttaataga
     3901 ttttgatttt taaaatggag gaattgaagt gtttcatcat atcagcgtta tgttaaacga
     3961 aaccattgat tatttaaatg taaaagaaaa tggtgtgtac attgactgta cgctaggtgg
     4021 agcgggacat gccctttatt tactaaatca attaaatgac gacggaagat taatagcaat
     4081 cgatcaagac caaactgcaa ttgataatgc taaagaggta ttaaaggatc atttgcataa
     4141 ggtgactttt gttcatagca acttccgtga attaactcaa atattaaaag acttaaacat
     4201 tgaaaaagta gatggaatct attacgactt gggtgtttca agcccacaac tcgacattcc
     4261 agaacgagga ttcagttatc accatgacgc aacattagac atgcgtatgg accaaacaca
     4321 agaactaaca gcatatgaaa ttgttaacaa ttggtcatat gaagcgttag tgaagatttt
     4381 ttatcgctat ggcgaggaga aattttcaaa acagatagct cgaagaatcg aagcacatcg
     4441 cgaacaacaa ccaataacaa caacattaga attagttgac attataaaag aaggtattcc
     4501 tgcaaaagcg agaagaaaag gcggacatcc tgcaaaacga gtatttcaag cactacgaat
     4561 tgcagtaaac gatgaattgt cagcttttga agattcaata gaacaagcga ttgaattagt
     4621 gaaagtagac ggcaggattt cggtaatcac tttccattct ttagaagatc gtttatgtaa
     4681 acaggtgttc caagaatatg aaaaaggtcc agaggtacca agaggattac cagttatacc
     4741 agaagcatat acacctaagt taaagcgtgt taatcgtaaa ccgattaccg ctacagaaga
     4801 agatttagat gacaataaca gagcacgaag cgcgaaatta cgtgtagctg aaatacttaa
     4861 ataaggagca attaataatg gctgtagaaa aagtgtacca accatatgac gaacaagttt
     4921 ataatagtat accgaagcaa caaccacaaa ctaagcccga aaagaagact gtttcgagaa
     4981 aagtggttgt acaattaact aaatttgaaa aagttttata cataactttg attactgtaa
     5041 ttgctatgtt aagtatttat atgctatctt taaaaatgga tgcgtatgat acgcgaggaa
     5101 agattgcaga tttagattat aaaatagata aacaatcaag tgaaaacagt gctttacaat
     5161 ctgaaatcaa aaagaattct tcttatgaac gcatatacga aaaggctaag aaacagggga
     5221 tgagccttga gaacgataat gtaaaggtag tgcgtagtaa tggcgaagca aaaaattaaa
     5281 attaaaaaaa ataaaatagg ggcagtccta cttgttggtt tattcggact gctctttttt
     5341 atattggttt taagaatttc atatatcatg attactggac attctaatgg tcaagattta
     5401 gtcatgaagg caaatgagaa gtatttagtt aagaatgcac aacaaccaga acgaggaaag
     5461 atatatgatc gtaatggtaa agtgctagca gaagatgtag aaagatataa acttgttgca
     5521 gtaatagata aaaaggcgag tgccaattct aaaaaaccta ggcatgtagt tgataaaaaa
     5581 gagactgcaa agaaattatc tacagtcatt gatatgaagc cagaggaaat tgaaaagaga
     5641 cttagtcaaa agaaagcctt ccaaattgaa tttggacgca aaggaacaaa tttaacgtat
     5701 caggacaaat tgaaaataga gaaaatgaat ttgcctggta tttctttatt gcctgaaaca
     5761 gaacgatttt atccaaatgg caattttgca tcacacttaa ttggtagagc tcagaaaaat
     5821 ccggatactg gtgaacttaa aggtgcgctt ggagttgaaa agatttttga tagttattta
     5881 agtggatcta aaggatcttt gagatatatt catgatattt ggggatatat tgcaccaaat
     5941 actaaaaaag agaagcagcc taaacgtggt gatgatgtcc atttaacaat cgattcaaat
     6001 attcaagtat ttgttgaaga ggctttagat ggcatggttg aaagatacca gccgaaagat
     6061 ttatttgcgg ttgtcatgga tgccaaaact ggagaaattt tagcatacag tcagcgacca
     6121 acatttaatc ctgaaactgg taaagatttt ggtaaaaagt gggcaaatga tctatatcaa
     6181 aacacatatg agcctggatc aacatttaaa tcatatgggt tagcagctgc tattcaagaa
     6241 ggtgcttttg atcctgataa gaaatataaa tctggacata gagatattat gggttcacgt
     6301 atttcagact ggaatagagt cggttggggt gaaatcccaa tgtcactcgg atttacttat
     6361 tcatctaata cattgatgat gcatttacaa gatttagttg gtgcagacaa aatgaaatct
     6421 tggtatgaac gatttggatt tggaaaatca actaaaggta tgtttgatgg agaagcacct
     6481 ggtcaaattg gatggagtaa tgagttacaa caaaaaacgt catcatttgg tcaatcgaca
     6541 actgtaacac ctgttcaaat gttacaggcg caatcagcgt tctttaatga tggtaatatg
     6601 ttaaaaccat ggtttgtgaa tagcgttgaa aatcctgtta gtaaaagaca attttataaa
     6661 ggtcaaaaac aaatcgcagg caaaccaata acaaaagata ctgctgaaaa agttgaaaag
     6721 caattggatt tagttgtgaa tagtaagaag agtcacgctg caaactatcg tattgatggt
     6781 tatgaggtcg aaggtaagac tggtacagca caagtcgctg cacctaatgg tggtggatac
     6841 gttaaaggtc caaacccata tttcgtaagt tttatgggtg acgcaccgaa gaaaaatcct
     6901 aaagttattg tatacgctgg tatgagcttg gcacaaaaaa atgaccaaga agcttatgaa
     6961 ttaggtgtta gtaaagcgtt taaaccaata atggaaaata ctttgaaata tttaaatgta
     7021 ggtaaatcaa aagatgacac atctaatgca gagtatagta aagtgccaga tgttgaaggt
     7081 caagacaaac aaaaagctat tgataatgtg agtgcaaaat cattagaacc agttactatc
     7141 ggttctggca cacaaataaa agcgcaatct ataaaagcag ggaataaagt cttacctcat
     7201 agtaaagtac tgttattaac agatggagac ttaactatgc ctgacatgtc aggatggacg
     7261 aaagaagatg tcattgcttt tgaaaaccta acaaatatta aagtgaattt aaaaggtagc
     7321 ggttttgtgt cccaccaatc aattagtaag ggacaaaaac ttactgaaaa agataaaata
     7381 gacgtagaat tttcatcaga gaatgtagac agcaattcga cgaataattc tgattcaaat
     7441 tcagatgata agaagaaatc tgacagtaaa actgacaaag ataagtcgga ctaacaagaa
     7501 tggctacatt aaataagggt gtattgtcac tatttgtttt tactaaagta atgctgtgat
     7561 agaaaacaac atcttcattt aatctttata aataagtgtt gatagagaat tttaaggtag
     7621 acgaggtcag attaacttgt ttgttatagg caaacttttt atactggcct cttatgccgt
     7681 aaaaaagaaa aattataaat tttgattaca actttgttta tttaattgaa aaattgataa
     7741 tatattagtg gttaaaaaag tctggattaa gaagaggaga tagttatgat ttttgtatat
     7801 gcgttattag cgctagtgat tacatttgtt ttggtacctg ttttaatacc tacattaaaa
     7861 aggatgaaat ttggtcaaag tattcgagaa gaaggcccac aaagccatat gaagaagact
     7921 ggtacaccaa cgatgggtgg actaacattt ctattaagta ttgtgataac gtctttggtg
     7981 gctattatat ttgtagatca agctaatcca atcatactgt tattatttgt gacgattggt
     8041 tttgggttaa ttggttttat agatgattat attattgttg ttaaaaagaa taaccaaggt
     8101 ttaacaagta aacagaagtt tttggcgcaa attggtattg cgattatttt ctttgtttta
     8161 agtaatgtat ttcatttggt gaatttttct acgagcatac atattccatt tacgaatgta
     8221 gcaatcccac tatcatttgc atatgttatt ttcattgttt tttggcaagt aggtttttct
     8281 aatgcggtaa atttaacaga tggtttagat ggattagcaa ctggactgtc aattatcgga
     8341 tttacaatgt atgccatcat gagctttgtg ttaggagaaa cggcgattgg tattttctgt
     8401 atcattatgt tgtttgcact tttaggattt ttaccatata acattaaccc tgctaaagtg
     8461 tttatgggag atacaggtag cttagcttta ggtggtatat ttgctacgat ttcaatcatg
     8521 cttaatcagg aattatcatt aatttttata ggtttagtat tcgtaattga aacattatct
     8581 gttatgttac aagtcgctag ctttaaattg actggaaagc gtatatttaa aatgagtccg
     8641 attcatcatc attttgaatt gataggttgg agtgaatgga aagtagttac agtattttgg
     8701 gctgttggtc tgatttcagg tttaatcggt ttatggattg gagtgcatta agatgcttaa
     8761 ttacacaggg ttagaaaata aaaatgtatt agtcgtcggt ttggcaaaaa gtggttatga
     8821 agcagctaaa ttattaagta aattaggtgc gaatgtgact gtcaatgatg ggaaagactt
     8881 atcacaagat gctcatgcaa aagatttaga atctatgggc atttctgttg taagtggaag
     8941 tcatccatta acattacttg ataaaaatcc aataattgtt aaaaatcctg gaataccata
     9001 tacagtatct attattgatg aagcagtgaa acgaggtttg aaaattttaa cagaagttga
     9061 gttaagttat ctaatctctg aagcaccaat catagctgta acaggtacaa atggtaaaac
     9121 gacagttact tctctaattg gagatatgtt taaaaaaagt cgcttaactg gaagattatc
     9181 cggcaatatt ggttatgttg catctaaagt agcacaagaa gtaaagccta cagattattt
     9241 agttacagag ttgtcgtcat tccagttact tggaatcgaa aagtataaac cacacattgc
     9301 tataattact aacatttatt cagcgcatct agattaccat gaaaatttag aaaactatca
     9361 aaatgctaaa aagcaaatat ataaaaatca aacggaagaa gattatttga tttgtaatta
     9421 tcatcaaaga caagtgatag agtcggaaga attaaaagct aagacattgt atttctcaac
     9481 acaacaagaa gttgatggta tttatattaa agatggtttt atcgtttata aaggtgttcg
     9541 tattattaac actgaagatt tagtattgcc tggtgaacat aatttagaaa atatattagc
     9601 agctgtgctt gcttgtattt tagctggtgt acctattaaa gcaattattg atagtttaac
     9661 tacattttca ggaatagagc atagattgca atatgttggt actaatagaa ctaataaata
     9721 ttataatgat tccaaagcaa caaacacgct agcaacacag tttgccttaa attcatttaa
     9781 tcaaccaatc atttggttat gtggtggttt ggatcgaggg aatgaatttg acgaactcat
     9841 tccttatatg gaaaatgttc gcgcgatggt tgtattcgga caaacgaaag ctaagtttgc
     9901 taaactaggt aatagtcaag ggaaatcggt cattgaagcg aacaatgtcg aagacgctgt
     9961 tgataaagta caagatatta tagaacccaa tgatgttgta ttattgtcac ctgcttgtgc
    10021 aagttgggat caatatagta cttttgaaga gcgtggagag aaatttattg aaagattccg
    10081 tgcccattta ccatcttatt aaagggtgtg agtattgatg gatgataaaa cgaagaacga
    10141 tcaacaagaa tcaaatgaag ataaagatga attagaatta tttacgagga atacatctaa
    10201 gaaaagacgg caaagaaaaa gatcaaaggc tacacatttt tctaatcaaa ataaagatga
    10261 tacatctcaa caagctgatt ttgatgaaga aatttacttg ataaataaag acttcaaaaa
    10321 agaacaaggc aatgaagaaa ataatgattc tgcttctagt catgcgaatg ataataatat
    10381 cgatgattct acagactcta atattgaaaa tgaggattat agatataatc aagaaactga
    10441 cgaccaaaat gaatcgaatg gaattgcagt cgacaacgaa caacatcaat cagctccaaa
    10501 tgaacaaaat agcgactcga atgatgagga aacagtaacg aaaaaagaac gaaaaagtaa
    10561 agtaacacaa ttaaaaccat taacacttga agaaaagcgg aaggtaagac gtaaacgaca
    10621 aaaacgaatc caatacagtg ttattacaat attggtattg ttgattgctg ttatattaat
    10681 ttacatgttt tcaccactta gtaaaattgc gcatgtaaat ataaatggaa ataatcacgt
    10741 tagtacttca aagataaaca aagttttagg tgttaaaaat gattcgagga tgtatacgtt
    10801 tagtaaaaaa aatgctatta atgatctcga agaggatcca ttaatcaaaa gtgttgagat
    10861 acacaagcaa ttaccaaaca cattaaacgt agatatcaca gaaaatgaaa ttattgcttt
    10921 agtgaaatat aaaggtaaat atttaccttt attagaaaat ggtaaattgc ttaaaggttc
    10981 aaatgatgtc aaaattaatg atgcacctgt catggatggt ttcaaaggta caaaagaaga
    11041 tgatatgatt aaggcgttat ctgaaatgac acctgaagtt agacgatata ttgccgaagt
    11101 gacatacgcc ccaagtaaaa acaaacaaag cagaattgaa ttgtttacga cggatggact
    11161 tcaagtaatc ggtgatattt cgacgatatc taagaaaatg aaatattatc cgcagatgtc
    11221 acaatcatta gcaagagata gttcgggtaa actaaaaaca cgaggctata ttgatttatc
    11281 agtcggtgct tcatttatcc cataccgtgg aaacacgtct actcaatcag aaagcgataa
    11341 aaatgtgact aaatcatctc aagaggaaaa tcaagcaaaa gaagaattac aaagcgtttt
    11401 aaacaagatt aacaaacaat caagtaagaa taattaaaaa attttgatat tgtctatgtt
    11461 tatagttcac aagccattca acgtattgta aactaaggat agtgtatttt tttaatagta
    11521 atttgtcagg aggtgcctat ctatggaaga acattactac gtaagtattg atattggatc
    11581 atcaagcgta aaaacaatag taggcgagaa atttcacaat ggtataaatg tgataggtac
    11641 aggacaaacc tacacgagcg gtataaaaaa tggtttaatt gatgattttg atattgcgcg
    11701 acaagcaatc aaagacacaa ttaaaaaggc atcaatcgct tcgggtgttg atattaaaga
    11761 agttttcctg aaattaccta tcattggaac ggaagtttat gatgaatcaa atgaaatcga
    11821 cttttatgag gatacagaaa tcaacggttc acatatcgaa aaagtattag aaggtattag
    11881 agaaaaaaat gatgtgcaag aaacagaagt aattaatgta ttcccaattc gttttatagt
    11941 cgataaagaa aatgaggttt cagaccctaa agaattaatt gccagacatt cattaaaggt
    12001 tgaagcaggc gtaattgcta ttcaaaaatc gattttaatt aatatgatta aatgcgttga
    12061 agcatgtggt gttgatgtat tagatgttta ttctgatgcg tataactatg gttcaatcct
    12121 aacagctact gaaaaagagt taggtgcatg tgtcattgat attggtgaag acgttacgca
    12181 agttgctttt tatgaacgcg gtgaattagt agatgctgat tctatcgaaa tggcagggcg
    12241 tgatattaca gacgatattg cacaaggatt aaacacttct tatgaaactg ctgaaaaagt
    12301 taaacaccaa tacggtcatg cattctatga ttctgcttca gatcaagata tcttcactgt
    12361 tgaacaggtt gatagtgatg aaacagtaca gtatactcaa aaagatttga gtgactttat
    12421 tgaagcgcgt gtagaagaaa tattcttcga agtatttgat gttttacaag atttaggatt
    12481 aacaaaagta aatggtgggt ttattgtaac tggtggatct gcaaacttac ttggcgtaaa
    12541 agaattatta tcagatatgg taagtgaaaa agttagaatt cacacgccat cacaaatggg
    12601 aattagaaaa cctgaatttt cttcagcaat ttctacaatt tctagtagta tcgcttttga
    12661 tgagttatta gattatgtta caattaatta tcatgataat gaagaaactg aagaagatgt
    12721 tattgatgtg aaagacaaag ataacgaatc taaattaggc ggatttgatt ggtttaaacg
    12781 taaaacaaac aaaaaagata ctcatgaaaa tgaagtagag tcatcagatg aagaaattta
    12841 tcaatcagaa gataatcatc aggaacataa acagaatcat gaacatgttc aagacaaaga
    12901 taaagaagaa agtaaattca aaaaactaat gaaatctcta tttgaatgat tattggccaa
    12961 taaaactagg aggaaattta aatgttagaa tttgaacaag gatttaatca tttagcgact
    13021 ttaaaggtca ttggtgtagg tggtggcggt aacaacgccg taaaccgaat gattgaccat
    13081 ggaatgaata atgttgaatt tatcgctatc aacacagacg gtcaagcttt aaacttatct
    13141 aaagctgaat ctaaaatcca aatcggtgaa aaattaacac gaggtttagg agcaggagct
    13201 aaccctgaaa tcggtaaaaa agctgcagag gaatctcgtg aacaaattga agatgcaatc
    13261 caaggtgcag acatggtatt tgttacttct ggtatgggtg gcggaactgg tactggtgca
    13321 gcaccagtcg ttgctaaaat tgcaaaagaa atgggcgcat taactgttgg tgttgtaact
    13381 cgtccattta gttttgaagg acgtaaacgt caaactcaag ctgctgctgg agtagaagct
    13441 atgaaagctg cagtagatac attaatcgtt ataccaaatg accgtttatt agatatcgtt
    13501 gacaaatcta cgccaatgat ggaagcattt aaagaagctg ataacgtgtt acgccaaggt
    13561 gtacaaggta tctcagactt aatcgctgtt tctggtgaag taaacttaga ctttgcagac
    13621 gttaagacaa ttatgtctaa ccaaggttct gcattaatgg gtattggtgt ttcttctggt
    13681 gaaaatagag cggtagaagc tgctaaaaaa gcaatctctt ctccattact tgaaacatct
    13741 atcgttggtg cacaaggtgt gcttatgaat attactggtg gcgagtcatt gtcattattt
    13801 gaagcacaag aggctgctga tattgtccaa gatgctgcag atgaagacgt taatatgatt
    13861 ttcggtacag ttattaatcc tgaattacaa gatgagattg ttgtaacagt tattgcaact
    13921 ggttttgatg acaaaccaac atcacatggt cgtaaatctg gtagcactgg attcggaaca
    13981 agcgtaaata cttctagcaa tgcaacttct aaagatgaat cattcacttc aaattcatca
    14041 aatgcacaag caactgatag tgtaagtgaa agaacacata caactaaaga agatgatatt
    14101 cctagcttca ttagaaatag agaagaaaga cgttcaagaa gaacaagacg ttaatcggtt
    14161 aatatataca caaataattc aacacaaatc atcagataac atatctgatg atttttttac
    14221 taattttta
//
LOCUS       DATTQF010000061        14182 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.061, whole genome shotgun sequence.
ACCESSION   DATTQF010000061 DATTQF010000000
VERSION     DATTQF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.061"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..757
                     /locus_tag="VB241_001497"
     CDS             <1..757
                     /locus_tag="VB241_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1244794.1"
                     /translation="KFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGYINE
                     NYIELLDVENNEKLKKMAKTIEAGVAFATQIVQEKYEQGFKNICLRTNDIEAVKNKLQ
                     SEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKKLQK
                     YFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIEDGK
                     VSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(749..1000)
                     /locus_tag="VB241_001498"
     CDS             complement(749..1000)
                     /locus_tag="VB241_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244795.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIALAIIHMGILAHSTYVYLY"
     gene            1389..2654
                     /gene="femX"
                     /locus_tag="VB241_001499"
                     /gene_synonym="fmhB"
     CDS             1389..2654
                     /gene="femX"
                     /locus_tag="VB241_001499"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HEP1244796.1"
                     /translation="MEKMHITNQEHDAFVKSNPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEIQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2771..5938
                     /locus_tag="VB241_001500"
     CDS             2771..5938
                     /locus_tag="VB241_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEP1244797.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTSMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKTSASNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVTTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKIDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6125..6442)
                     /gene="mspA"
                     /locus_tag="VB241_001501"
     CDS             complement(6125..6442)
                     /gene="mspA"
                     /locus_tag="VB241_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HEP1244798.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6687..6851)
                     /locus_tag="VB241_001502"
     CDS             complement(6687..6851)
                     /locus_tag="VB241_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244799.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            6984..7892
                     /locus_tag="VB241_001503"
     CDS             6984..7892
                     /locus_tag="VB241_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEP1244800.1"
                     /translation="MTEQFTMIILLIALGYFLKRINYIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSLSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8063..8203
                     /locus_tag="VB241_001504"
     CDS             8063..8203
                     /locus_tag="VB241_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244801.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLNRMINVMMCYCVLFLEPCISW
                     DI"
     gene            complement(8311..9174)
                     /locus_tag="VB241_001505"
     CDS             complement(8311..9174)
                     /locus_tag="VB241_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HEP1244802.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWVGIIII
                     VIAAIILGNLK"
     gene            complement(9355..10242)
                     /locus_tag="VB241_001506"
     CDS             complement(9355..10242)
                     /locus_tag="VB241_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1244803.1"
                     /translation="MSIVQLYDITQIKSFIKHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVETFETLFKTMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            10308..10403
                     /locus_tag="VB241_001507"
     CDS             10308..10403
                     /locus_tag="VB241_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244804.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            10429..12564
                     /locus_tag="VB241_001508"
     CDS             10429..12564
                     /locus_tag="VB241_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEP1244805.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPLRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMSPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKVMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            12677..14011
                     /locus_tag="VB241_001509"
     CDS             12677..14011
                     /locus_tag="VB241_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP1244806.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
BASE COUNT         5140 a         2050 c         2575 g         4417 t
ORIGIN      
        1 gaaatttgat cacatcattc attatataga tcagttagat cggtttagtt ttccaggaga
       61 tgttataaaa ttacattcag gtgggtatca tcataaatat ggaacattca ataaattagg
      121 ttatatcaat gaaaattata ttgagctact agatgtagaa aataatgaaa agttgaaaaa
      181 gatggcaaaa acgatagaag ctggagtcgc ttttgctact caaattgttc aagagaagta
      241 tgagcaaggc tttaaaaata tttgtttgcg tacaaatgat atagaggcag ttaaaaataa
      301 actacaaagt gagcaggttg aagtagtagg accgattcaa atggaaagag atacacataa
      361 agatggtaag gtaaagtggc aattgcttta tataatgaat caggatgatg atgaaattaa
      421 gccaccattt tttattcaat gggaagaaag tgattccatg cgtactaaaa aattacaaaa
      481 atattttcaa aaacaatttt caattgaaac tgttattgtg aaaagtaaaa accgatcaca
      541 aacagtatcg aattggttga aatggtttga tatggacatt gtagaagaga acgaccatta
      601 cacagatttg attttaaaaa atgatgatat ttattttaga attgaagatg gtaaagtttc
      661 aaaatatcat tcggttataa taaaagacgc acaagcaact tcaccatatt caatttttat
      721 cagaggtgct atttatcgct ttgaaccatt agtataaata tacgtaagtg ctatgagcga
      781 gaatgcccat atgaataatg gcaagcgcaa tggaaagaat cgttaatata ttatttaatc
      841 gtgatgactt aattaaaatg aaaaagattg ataatataaa tgtgaaaaag ataagtataa
      901 cccgtaaact aaagtaattc acggtgagag gttgactcaa tgtcataatg attgcaacga
      961 tgttcataat tataaataga cttaaaataa ttgttctcat atcaaacacc tcattgttag
     1021 attattgaca ttataacagg ggtaattgta tatgaacatt aatgtggttg cttgaggaga
     1081 aatttattca ttgaagtcaa attggttcat gttagaaatg aatatcgtgt tagatgatga
     1141 aagtatattg aagtataggt aactagttga aaagtattaa ttgtacgata acattaaatt
     1201 taacaggaag catagatata aaatgattca caattaaaat gagtaaattt gaacttgcta
     1261 aactattaat tggagcatgg acatttcaaa aataagagtt caaatcttac acaagctctg
     1321 aatcgacact ataagataca aactgtataa ttaaaggtat tgttaaatag aaggagatat
     1381 cataaatcat ggaaaagatg catatcacta atcaggaaca tgacgcattt gttaaatcta
     1441 acccaaatgg agatttatta caattaacga aatgggcaga aacaaaaaaa ttaactggat
     1501 ggtacgcgcg aagaatcgct gtaggtcgtg acggtgaaat tcagggtgtt gcgcagttac
     1561 tttttaaaaa agtacctaaa ttaccttata cgctatgtta tatttcacgt ggttttgttg
     1621 ttgattatag taataaagag gcgttaaatg cattgttaga cagtgcaaaa gaaattgcta
     1681 aagctgagaa agcgtatgca attaaaatcg atcctgatgt tgaagttgat aaaggtacag
     1741 atgctttgca aaatttgaaa gcgcttggtt ttaaacataa aggatttaaa gaaggtttat
     1801 caaaagacta catccaacca cgtatgacta tgattacacc aattgataaa aatgatgatg
     1861 agttattaaa tagcttcgaa cgccgaaatc gttcaaaagt gcgcttggct ttaaaacgag
     1921 gtacgacagt agaacgatct gatagagaag gtttaaaaac atttgctgaa ttaatgaaaa
     1981 tcactgggga acgcgatggc ttcttaacgc gtgatattag ttactttgaa aatatttatg
     2041 atgcgttgca tgaagatgga gatgctgaac tatttttagt aaagttggat ccaaaagaaa
     2101 atatagcgaa agtaaatcaa gaattgaatg aacttcatgc cgaaattgct aaatggcagc
     2161 agaagatgga aacatctgaa aagcaagcta aaaaagcgca aaatatgatt aatgatgcgc
     2221 aaaataaaat tgctaaaaat gaagatttaa aacgagacct agaagcttta gaaaaggaac
     2281 atcctgaagg tatttatctt tctggtgcac tattaatgtt tgctggctca aaatcatatt
     2341 acttatatgg tgcgtcttct aatgaattta gagatttttt accaaatcat catatgcagt
     2401 atacgatgat gaagtatgca cgtgaacatg gtgcaacaac ttacgatttc ggtggtacag
     2461 ataatgatcc agataaagac tcagaacatt atggattatg ggcatttaaa aaagtgtggg
     2521 gaacatactt aagtgaaaag attggtgaat ttgattatgt attgaatcag ccattgtacc
     2581 aattaattga gcaagttaaa ccgcgtttaa caaaagctaa aattaaaata tctcgtaaat
     2641 taaaacgaaa atagattaac gactgaaatc tgaacgctca taagactgtc atttgcgttc
     2701 agattttttt acacaatata gaatggttga gtaaaatatt tttgaatata gtgaaagagg
     2761 gggaagtact gtgataaaaa agctattaca attttctttg gggaataagt ttgctatctt
     2821 tttaatggtt gttttagttg tcttgggcgg tgtatacgcg agtgctaaat tgaaattaga
     2881 attactacca aatgtacaaa atccagttat ttcagttaca acatcaatgc cgggtgcaac
     2941 gccacaaagt acccaagatg aaataagtag taaaattgac aatcaagtaa gatcgttggc
     3001 atatgtgaaa aatgttaaaa cgcaatccat acaaaatgct tcaattgtaa cagttgaata
     3061 tgaaaataat acagatatgg ataaagcaga agaacagctt aaaaaagaaa tcgataaaat
     3121 taaatttaaa gatgaagttg gtcaaccaga attaagacgt aattcgatgg acgcttttcc
     3181 ggttttagca tattcatttt caaataaaga gaatgacttg aaaaaagtaa cgaaagtact
     3241 gaatgaacaa ttaataccaa aattacaaac ggtagatggt gtgcaaaatg cgcaattaaa
     3301 tgggcagacg aaccgtgaaa tcacccttaa atttaagcaa aatgaacttg aaaaatatgg
     3361 gttgactgct gatgatgtag aaaactatct aaaaacggca acaagaacaa cgccacttgg
     3421 attgttccaa tttggtgata aagataaatc aattgttgtt gatggtcaat atcaatctgt
     3481 tgatgctttt aaaaacataa atattccatt aacgttggca ggagggcaag ggcaatctca
     3541 atcccaaagt gacaataaac aaaattcagc catgtcagac gttaatcagg catcaccgca
     3601 gcaaaattca aaaacgtcag catcaaataa tataagtggt atgccgacag cgaaactagg
     3661 agatttagct gatattacag ttggtgatgt gcgtacttct atttctaaaa cgaatggcaa
     3721 ggatgcggtt aatctacaaa taactaaagc tcaagatgcc aatacagttc aagtagccaa
     3781 agatgtacag cgtaaaattg atacattcgt tgatgaaaat aaagatttta atgtcacaaa
     3841 aacaatggat actgcaaagc ctgttgagaa atcactttat acgatggttg aaaaagcatc
     3901 attaggtaca atcgtggcaa ttatagttat tttgctgttt ttaagaaaca ttcgtacgac
     3961 ggcaatttct attatatcca ttccattatc acttcttatg gcgcttattg ctctgaaatt
     4021 gagtgatgtt tcattgaata tactaacgtt aggtgcatta acagtagcga ttggacgtgt
     4081 gatagacgat tcgattgtag ttgttgaaaa tatttatcga cgcttaacag attcagaaga
     4141 acaactaaaa ggtgaaaatt taattatcag tgcgacaact gaagtattta aaccaataat
     4201 gtcatcgaca ctagttacta ttatcgtctt cttaccactt gtgtttgtat caggttcagt
     4261 aggagaaatg tttagacctt ttgcattggc tattgcattt agtttattag catcgttatt
     4321 agtgtcaatt acactcgttc cagcgttggc agcaacacta tttaaaaaag gcgttaaacg
     4381 tcgtaataaa caacatcaag aaggattagg tgttgttagt acaacttata aaaaagtatt
     4441 gcattggtca ctaaatcata agtggattgt aattatatta agtacattaa ttttggttac
     4501 aactattgta tttggaggac cgagactagg cactagcttt atttcagcag gtgacgataa
     4561 atttttagct attacttata caccgaagcc tggtgaaacg gagcaagcag tgttgaatca
     4621 tgcgaaagat gttgaaaaat atttaaaaca gaaaaagcat gtaaaaacaa ttcaatactc
     4681 agttggcggt agtagtccag tagatccaac gggtagtaca aatagtatgg caatcatggt
     4741 tgaatatgat aatgacacgc ctaattttga tgtagaagcg gataaggtta ttaaacatgc
     4801 agatggcttt aaacatcctg gagagtggaa aaatcaagat ttaggaacag gtgcaggtaa
     4861 taaatctgta gaggttactg tgaaaggtcc atctatggat gccataaaat caactgtaaa
     4921 agatattgaa cagaaaatga aacaggttaa aggtctagcc aatgtcaaat ctgatttatc
     4981 acaaacatat gatcagtatg aaattaaaat tgatcaaaat aaagcggcag aaaatggtat
     5041 ttctgcaagt caacttgcaa tgcacttgaa tgaaaactta ccagaaaaaa cagttacgac
     5101 tgttaaagaa aatggtaaaa ctgttgatgt taaagtcaaa caaaataagc aaacagactg
     5161 gtcagaagat aagttgaata atataacttt gaaaaagccg actggtggta cgattaaatt
     5221 gggagatatc gctacgttag ttaaaacaac gacaccaagt aaattgacgc aagaacaagg
     5281 tgattatgca acgacggtat ctgctaaagt aacaaataaa gatgtgggtg gcacaacacg
     5341 acaagtgatg tctaaaataa ataatttgga caaaccgaat aatgtaaagg ttaatatcgg
     5401 tggtgcatca gatgatatta acaatgcaat gacacaatta gcctttgcaa tgttagctgc
     5461 aatcattatc gtatatttaa tcctagttat tacatttaaa ggtggcttag caccatttac
     5521 aattttattc tctttaccat ttacagttat cggtgtaatt attgcactat taatcacagg
     5581 agaaacaata tcagtaccaa gtttaattgg tatgctaatg ttaattggaa ttgtagtaac
     5641 aaatgccatt gtgttaatag accgtgtcat taataatgag cagcagggca tggagatgaa
     5701 agaagcatta atcgaagcag gcggtactag aattagaccg atattaatga cggcgattgc
     5761 aacaatcggt gcattagttc ctttgttatt tggtcaagat agctcgattc ttatttcgaa
     5821 aggattagcc gctacagtta ttggtggttt aatttcatca actttattaa cacttgtagt
     5881 tgtaccagta atatatgaaa ttctctttac tttaaaaaaa cgattcacta aacgatagat
     5941 aatataaaag ccataaaagc ggtcatgatg ttgactactt ttatggcttt ttaaaatttg
     6001 aaattttaga ccttatttca cgtgcgattt aatcgatgtg ttaataaaac aatactggca
     6061 ttcgatatgt aatctgatga taatataagg ttgcaattat gttattgcat gttttgttta
     6121 ttacttagaa tagtacgatg gttaagatga aatatatgac gaagataaat aatgacatta
     6181 aattcaagat gtttacgcct tttagatctt ttttaagcat tttaaatcct gtcacttcga
     6241 cattaccaac taagagtaat aagacgataa atacccacca attctctttt ggaaaactca
     6301 tcgtcgttaa tgctaagacg aataataaca acacacccat aataattctc aaaattcgag
     6361 taaatacaac ttccatttta aaaatactaa taaaactaac aattagataa agtattgcta
     6421 gtaaaatcag ataaaattgc atatgataac ttcctctccg tttcaaaggg ttcatgtagt
     6481 acagtagcgt agaaaacata aatagcagtg ttgggataga tatgcgcgta tgttgttaat
     6541 gagacactaa atcataacac gacaacatct tgtgattcta gatgtaattg agcgaaaaat
     6601 gaagcaattc atcaatataa cgtattcaga gcaatgctag tcaaatttat gtaatgaaaa
     6661 gcatttattg tctgaatgat agattattaa tcttgcattt gagcacgaac gtttctaatt
     6721 tcattttcaa tatctttaag ttgacgaaca agagcggaag tatctaaatt caaagattcc
     6781 cgtttttcta agtcaccttg aagacgaaca taatcatatt taagttcggc tagttggttt
     6841 tctaaagaca tactataaca ctcctttgaa atataattaa gtgcatttaa tgtagattat
     6901 agcatgaaac agtagacttc taaaatatct gtggagtatg ttaaagttat aaattatgaa
     6961 gattattgaa agaagggata acagtgacag aacaatttac tatgattatt ttattaattg
     7021 cgcttggtta ctttttgaag cgcattaatt atataaaggc aacagatagt caagtcattg
     7081 ctacattagt tcttaatgtc acactcccat ctttagttat tgtgaattta aacagtgcag
     7141 aattaaaatt gtcattatcc attttaccaa ttttaatgat tatctatggt attgtggcga
     7201 aaatgattgc tgtctggttt tttagaaaat atgataatca catgcgtgga tcagtaggta
     7261 tgatgactgg tgctatgaat attggattgt ttgcatatcc actaattgaa gtgatatggc
     7321 ctaaaacggg acttatttat tttggtatgg ctgatatagg tggtgccttt gtgatgtttg
     7381 gaattactta ctttgtaggt agctacttta gtgaaggtag tgatcaattc gattttaaat
     7441 atttaggtaa gaaattatta caatcagtgc cactcgttac atacattgta atgtttatat
     7501 tgaatataag tcacatccat ttaccacatg tggcaatcga tttctttagc atactttccc
     7561 atgcaaatat gccgttatca atgattttgc taggtgtcat gttgaatttt actctggaaa
     7621 gaaagtatat acctgctact attaaatatt tatgtttaca ttatggatta gcattagttg
     7681 cgggattgtt agtacatgtg ttgctaccgg tatcagatga tatgattaag acgacattgt
     7741 tggtcacttg gatgttcccg gttggtgtcg caattatttc ttatggtatt cagtttaaat
     7801 atcgaacact gccatttata ggtatgacga ccaatttaac aatcattatt agtatcatta
     7861 ttttgtacgt gtatcaggca gtgtttgtct agtttacaca tttgaaaata aaatccattt
     7921 tagtattcgt agaaaatgag agcccccttt accacaatat gtaaatgcat agtgtgaaaa
     7981 gggggctttt atttatgaca tagttgttca actcatacat tctagcagat aatgcagtgc
     8041 gttatgcagc tatccacttc atatgtatgg cgcatacctg ataaacgaat atcatcaaga
     8101 ctttaaaagt atcgctaatt ttaaagtctt aaaccgcatg attaatgtca tgatgtgtta
     8161 ttgcgttttg tttttagagc catgtatttc ctgggacata tagaggaaca ttcaacgtaa
     8221 aattcacgaa aattaatttt aaacattcta aaagtgaatg gttattttca gaaacgataa
     8281 catttttacg ttacatgagt atttaacttt ctatttcaaa ttacctagaa ttatagcagc
     8341 tatcacgata attataatac ctacccaaat accagtcatc tgacgacgat cttttctttc
     8401 gcctaaaatg aaaataccgc ctaatgttga aacgataaca agtaactgtg ataatgagaa
     8461 acttgtagct acaccaactt ttggttgaga atagaacatg aacaagttac caattcccca
     8521 aatcacacct ggcaatagat taagtgctgt tgatttaagt gatgttttat gattcatgga
     8581 taggataaag ccaccaattg ccataccgac agattggaag aacaatgcat cagttccacc
     8641 aacaccaaag atgtcaccaa gtacaacgaa acctacatat ccaactgtag atacaattaa
     8701 aatacccatt gcttttttaa attcaggatt atctgattga cgttcatttt tagctttaag
     8761 tgaagtaagt gctacaccag taactaataa tatcatggcg attaaaccaa agataatttg
     8821 agtcattgaa gaccattcac ctaaaaagat aacactgaat aatgttgtac caactaattg
     8881 catacctgta gaaactggca tagtatttga tacaccgatt aaactaatag atttaagctg
     8941 attcgcttgt ccaaacgccc ataatgcacc agaaataaga ccgacaataa ttacagtagg
     9001 attattgaat tttgcgaaac cagtaataag taaacctaat ccaacaatga gtgcgcctag
     9061 cgtcgtacca cgaatttggt tgtaaggtcc accgccgacg aacacattaa taagaacgac
     9121 acttccccag aataaagcag gtaaaagtgc gattaagaaa tcaagaaatt gcaagactac
     9181 tcactctcct atactattta tgcaagtgct ccccttaatt gacacttgca tgttattgta
     9241 aagcattctg ccctaatagt aaaaaacaac tacttataat tttcaatatt tacaaaaagt
     9301 agaaaaaccc acaatacatg ctaatgatgt actgtggggt atttgcttgg tattttattc
     9361 agggattgtt tgatctttta cgttacgtga ttcgaatttg aatttaatgt actcatagtt
     9421 gatatggaaa cctagaccat taaatcgttc gatgagttta ttatttttgt tacgaatttt
     9481 aaaataaact tttctaatat caaagttatc aaaagcatat tgcaatgcat acgcaagcaa
     9541 ctcgaaagcg atacctttca aacggtaatc tacatgagaa ctgaagtatt taatctcggc
     9601 gattgactgt tgtgaatcaa tttcaaggta taaataacct ttaagtaaac cttcgctaac
     9661 aaataaaaac aaatgatgat ggtcgtctaa actatcaatg atatcttgtg gtgactgggc
     9721 ctgatattta aatgtagttg tgtgtaattt gctgaaagca cgataaaacc ctttgtgata
     9781 aggaatgata tttggttggt gcatatcttc ttctaatctt gtacgggctt ctatgtaata
     9841 gtcagtgaag ttataacttg cttgaataac tttcattaat ggtttgtatt gttgaatgcc
     9901 ttcttcaaaa gaaaagttaa agacggcatc gtcaggttga ttcgaagtca ttgttttaaa
     9961 cagcgtttca aacgtttcga cagataatac ataattgtca gccacgaaag gacctatgac
    10021 tttgaattta tttttatcgt atgcaaaaga caatatgatt gctttaattg aatcgttttc
    10081 tttaattgaa aatacaccag gtgattcaat cgcatcggtt attaatacat ctatttcatt
    10141 gtactgttga ggaagtttat ataagtatga tgctgattca taattcgaat gtttaatgaa
    10201 cgattttatt tgtgtaatat catataactg aactatgctc atgtcattac ctccgtactt
    10261 tttgttactt ttattatata gtatttcaac tgaaatgaaa gttaatagtg atattaacat
    10321 gttacaatac atttaacaca tcatttgcaa tcattgaatt taaatcaaag attagtggaa
    10381 tagatgaagc acgttcgaaa taaaagaaag tatgagaaag gataatttat gaaatcttta
    10441 atattagctg aaaaaccatc agtcgcaaga gatattgctg atgctttaca aataaatcaa
    10501 aagcgtaatg gttactttga aaataaccaa tatattgtca cgtgggcgtt aggtcatcta
    10561 gtgacaaatg cgacacctga acaatacgat aaaaatttaa aggaatggcg attagaagac
    10621 cttccaatta tacctaaata tatgaaaact gttgttattg gtaaaacaag caaacaattt
    10681 aaaacagtaa aagcgttaat tttagataat aaagtgaaag atattattat tgcaacagat
    10741 gctggacgag aaggtgaact agttgcaaga ttgattttgg ataaagttgg taacaaaaag
    10801 ccactccgtc gattatggat tagctcagtt actaaaaaag ctattcaaca aggttttaaa
    10861 aatttaaaag acggtcgcca atataacgat ttgtattatg cagcgttagc gagaagtgaa
    10921 gcggattgga ttgttgggat taatgcaacg cgtgcactaa caacaaagta tgatgcacag
    10981 ctatccctgg gacgtgttca gacaccaacg attcaattag taaatacacg acaacaagag
    11041 attaatcagt tcaaaccaca acaatactat acattatcat taacggtaaa agggtttgat
    11101 tttcagctag aatcaaatca gcgatatacc aataaagaaa ctttagaaca gatagttaat
    11161 aatttgaaaa atgtcgatgg taagattaaa tctgttgcta ctaaacataa gaagtcgtat
    11221 ccgcaatcac tgtacaattt aacagattta caacaagata tgtatagacg ttataaaatt
    11281 ggacctaaag aaacattgaa tacacttcaa agtttatatg agagacataa agtcgtaacc
    11341 tatccaagaa cagattcaaa ctatttaaca actgatatgg tagatactat gaaagaacgt
    11401 attcaggcga cgatggcaac aacatataaa gaccaagcgc gcccattaat gtctaaaaca
    11461 ttttcatcaa aaatgtcgat atttaataat caaaaagtat ctgatcacca tgcaattatt
    11521 cctacagaag tgagacctgt catgtcagac ttaagtaata gagaattaaa gttatacgat
    11581 atgattgtcg agcgtttttt agaagcttta atgtctccgc acgagtatga cgcgataact
    11641 gttactttag aggttgcagg gcacacattt gttttgaaag agaatgtaac aactgtatta
    11701 ggctttaaat ctattagaca aggtgaatct attacagaga tgcaacagcc tttttcagaa
    11761 ggcgatgaag tgaagatttc aaaaacaaac attagagaac atgaaacaac acctccagaa
    11821 tattttaatg aaggttcgtt attaaaagtg atggagaacc ctcagaactt tattcaattg
    11881 aaggataaaa aatatgcgca aacattaaaa caaacaggtg gtatcggcac agttgcaaca
    11941 agggccgaca ttatcgataa attatttaat atgaatgcca ttgaatcaag agacggtaaa
    12001 attaaagtga cgtcaaaagg taaacaaata ttagaattag caccagaaga attaacgtcg
    12061 ccacttttaa ctgcacaatg ggaagaaaaa ttacttttaa ttgaacgtgg taaatatcag
    12121 gcgaaaacat tcattaatga aatgaaagat tttacgaaag atgttgtaaa tgggataaaa
    12181 aatagtgatc gtaaatataa acacgataat ttaacaacca ctgaatgccc aacgtgtggt
    12241 aaattcatga ttaaagttaa aactaaaaat ggtcagatgc ttgtgtgcca agatccatct
    12301 tgtaagacga aaaagaatgt acagcgcaaa acaaatgcaa gatgtccaaa ctgtaaaaag
    12361 aaattaacgt tgtttggtaa aggaaaagaa gcggtatatc gttgtgtttg tggacattct
    12421 gaaacgcaag cacatatgga tcagcgtatg aagtctaaat cctctggtaa agtatctcgt
    12481 aaagaaatga aaaagtatat gaataaaaat gaaggtttag acaataatcc gtttaaagat
    12541 gcattaaaaa acttgaattt atagataaaa tcgaacaaag ttgaatcata aaatcgaaaa
    12601 gttcgctttt ggtattgttt tttattaaga atgatattaa actattaagg tattttaaaa
    12661 aaaggagcat ccattcgtga aaaactattt ccagttcgat aaatatggaa caaactttaa
    12721 aagagaaata ttaggcggta tcacaacttt cttatctatg gcctatattt tagcagttaa
    12781 cccgcaagtt ttaagtttag caggtgttaa aggcgtatca gaagatatga aaatggacca
    12841 aggtgccatt tttgtagcga ctgcattagc agcatttgta ggctcgctat tcatggggct
    12901 aatagctaaa tatccaattg cgttagcacc aggtatggga ttaaatgcat tctttgcatt
    12961 tactgttgtg ttaaccatgg gtattccttg gcaagttggt ttaacaggtg ttttattctc
    13021 aggtatattc tttgcgatat taactgtcac agggtttagg gaagttatta ttaatgcaat
    13081 tccttatcaa atgaagatgg ctgtttcagc aggtataggg ttattcatta catttgtagg
    13141 tttacaaagt tcgggtatta tagttaaaaa tgaatctaca ttagtaacat tgggacattt
    13201 aactgatcca ccagtacttt tagcgatatt cggtatcgtt attacagtta ttttatatgc
    13261 aattaaattg ccaggttcta tctttatcgg tatgatcatc acagcaatcg ttggtatgtt
    13321 cactggattg attcaaatgc catctggtat tgtaggtaag attccaagta ttgaaccaac
    13381 gttcggcgct gcatttgaag cgtttaaaga cccaagtcaa ttattaacga tacagttctt
    13441 aattgttatt ttaacgttct tatttattga tttctttgat acagctggta cattagtagc
    13501 cgttgctact caagcaggta ttatgaaaga taataaatta ccaagagctg gtagagcatt
    13561 gttctcagat tctttagcta cgattgtagg atcagttttt ggaacaacta caacaacttc
    13621 ttatatcgaa tcaacttcag gtgttgctgt aggtgctaga acaggatttg ctagtatcgt
    13681 aacaggttgt tgtttcctat tagcgctatt ctttagtccg ttaatcgctg ttgtaacaag
    13741 tgcagttacg acaccagctt tagtggtggt aggtgtatta atggctgcaa actttgctga
    13801 aataaactgg aaaagttttg aagttgcagt gccagcattc attacaatca ttatgatgcc
    13861 tttatcatat tcaattgcaa ctggtattgc gtgtggattt attttctatc caattacaat
    13921 gcttatctct aaaaaacata aagaagtaca tccgataatg tatgtattaa tggtactatt
    13981 cattttgtac ttcatatttg ttcatggtta actccagaca caaaaaacga ctgccaatga
    14041 ttggtagtcg ttttttcttt gaagtaagga taaaataaga agcgaaataa cataaagcaa
    14101 tattatgaat gtctttaaat atccgatata aatatacttc ttttaaaatt gttgaacgtg
    14161 tttcttctat aatatatata cc
//
LOCUS       DATTQF010000062        13888 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.062, whole genome shotgun sequence.
ACCESSION   DATTQF010000062 DATTQF010000000
VERSION     DATTQF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.062"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(100..456)
                     /locus_tag="VB241_001510"
     CDS             complement(100..456)
                     /locus_tag="VB241_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEP1244807.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLYGAFKEKYEKELRDNETQKEAFEKLKAIVNQHHHVLLLYSAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(613..786)
                     /locus_tag="VB241_001511"
     CDS             complement(613..786)
                     /locus_tag="VB241_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244808.1"
                     /translation="MKLNQRYVKVFALYFASIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(811..1956)
                     /locus_tag="VB241_001512"
     CDS             complement(811..1956)
                     /locus_tag="VB241_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HEP1244809.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETDAYDFSQYVPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIANHYDNAKLYTHLKGEQGYIGAEELKA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVVV"
     gene            2111..2218
                     /locus_tag="VB241_001513"
     CDS             2111..2218
                     /locus_tag="VB241_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244810.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            2529..3482
                     /locus_tag="VB241_001514"
     CDS             2529..3482
                     /locus_tag="VB241_001514"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP1244811.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDAEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(3803..5332)
                     /locus_tag="VB241_001515"
     CDS             complement(3803..5332)
                     /locus_tag="VB241_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1244812.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            5696..6631
                     /locus_tag="VB241_001516"
     CDS             5696..6631
                     /locus_tag="VB241_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEP1244813.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIVVAKGADRPLIKLVAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADEHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            6741..6844
                     /locus_tag="VB241_001517"
                     /pseudo
     CDS             6741..6844
                     /locus_tag="VB241_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6969..9065
                     /locus_tag="VB241_001518"
                     /pseudo
     CDS             6969..9065
                     /locus_tag="VB241_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            9050..9517
                     /locus_tag="VB241_001519"
     CDS             9050..9517
                     /locus_tag="VB241_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP1244814.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLKYN"
     gene            9540..9818
                     /locus_tag="VB241_001520"
     CDS             9540..9818
                     /locus_tag="VB241_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP1244815.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            10045..11304
                     /locus_tag="VB241_001521"
     CDS             10045..11304
                     /locus_tag="VB241_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEP1244816.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQRVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            11322..12377
                     /locus_tag="VB241_001522"
     CDS             11322..12377
                     /locus_tag="VB241_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEP1244817.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEITFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            12379..12525
                     /locus_tag="VB241_001523"
     CDS             12379..12525
                     /locus_tag="VB241_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244818.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            12549..13592
                     /locus_tag="VB241_001524"
     CDS             12549..13592
                     /locus_tag="VB241_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEP1244819.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIQGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDIAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
BASE COUNT         4703 a         2154 c         2691 g         4340 t
ORIGIN      
        1 tattaaagtt caattaattg tagtaattac actttttaaa aataaatatt agaaatgaag
       61 ttagcgacat gtatagtgat ccacgataaa catatatatc taagtattga gcaactgctg
      121 tagtactaca gcttggttat gtttagtatc ttttgctgaa tataacaata gaacatgatg
      181 atgttgattt acaattgcct ttaatttttc aaaagcttct ttttgcgtct cattgtcacg
      241 caattctttt tcgtattttt ctttaaaagc accataaagt ttaggatcat gttggaacca
      301 ttgtcgtaac tcagtagaag gggcaatgtc ttttaaccaa taatctaggt tagcagttct
      361 tttcgaaata cctcttggcc agactctatc gactaggata cgaatagcgt ctgtattatc
      421 tttattgtca taaatccgtc caatatctac ggtcatcttg tgaactcctt tcttatgaaa
      481 ttcagtgagc atacatcaat gcatgttgtg gtgggacgac gaaataaatt ttgcgaaaat
      541 attatttctg tcccactccc aattaaaaaa caaccatgac aaagtaaagt catagctgtt
      601 tttgtataga tgtcatttat ttttacgttt agttaaatac ttcaaaccaa ctgcaaagac
      661 ggtgtaaccg gcaagtgttt gaatcaatgt tttgattaaa ttgttatttt tgacaacaat
      721 atttgcggta acaatactcg cgaaatataa tgcaaatact ttaacgtaac gttgattaag
      781 tttcatatga gcactaacct cttttctcag ttatactaca acgcttagtc ttggaataaa
      841 tgtttcgtag tgtacgcgat ccatatcgta atttaaagat ttaagtgctt cgatcataga
      901 ttgtaagaat tttgtaccac cacagatata aatttcaggt ttatttgcta aaaatgcttt
      961 taattcttca gcaccgatat aaccttgttc gccttttaag tgggtatata atttagcgtt
     1021 gtcataatga tttgcaatac tgttgaagtt gtctttgaaa ggtaaatgtt gttcattttc
     1081 agcaacttga accatctgag tatctaaacc tttggcagag gcagcttcat acattgctac
     1141 taaaggtgtg acgccaatac ctgaacctaa gaaaagttgt ggttcagtcg tattctctaa
     1201 tacgaatcca cctacaggtg cagctaaatt aatcatatcg ccttctttaa tctcatcgtg
     1261 taaaattgtt gaaacttcgc cttcatgttc tgttgtgaca tcacgtttaa cgccaaaggt
     1321 taaatgattt ttatcacctg atacgataga atagtgacgt ttagctctat atggaagttt
     1381 atcactagaa acatcaactg tgatgtattg gcctggtaca tattgactaa agtcatatgc
     1441 atcagtttca actgtaaatg atttaatgtc ttcagattct tgtttaatat tggtaatttt
     1501 gaatggttta aaaccaatcc acatcatttg atcataaatt tctttttcaa tttggatgaa
     1561 cacatccgca ataacgccat atgctttcgc ccaagcttga atgacagggt cattttcttc
     1621 taatcctgtc acgtcttgaa tggcttttaa taaattttcc cccacaattg gataatgttc
     1681 agcataaact tgtagtgcgc agtgtttata tgcaactggc ataataactg gtttaataac
     1741 acttaaatta tcgatattaa ccgctgcggc cattacagct tgtgctaatg ctgaagattg
     1801 catgcctctt ttttggttcg tttgattaaa catatttaaa agttcaggat gtgccttaaa
     1861 catttttgga taaaagattg acgtaatttc tgttcctttt tctttaagca atggcaccgt
     1921 ttgtttgata atgtctttct cttgttctgt aagcatgata ctcctccttt aatctgtcta
     1981 ttttgattat tctactaaaa attccgatat tcaattaatt ggtttgagaa aatataaata
     2041 aaatggcaaa tttgataatt gtatgacatt tttaattttt taaatactta tcaataagca
     2101 ttgtatacaa ttgtctgttt gtacaccgac gattgcgcgc atttatttga ctaattcaaa
     2161 aaacattgtt gttttcttag aaaaaagtaa acatgataat aaaaatgtga aagtgtaaat
     2221 aatcactggc gaagtacgaa gactaaagac atctaagatg taatcgtata caaattaaaa
     2281 aggtgtaaaa attaaaataa aatgtgaaat aaatcacaat ttaatattga cccagtactt
     2341 aatgcatgtt acattttata tgtgaaataa atcacaaact taaaagcgga tgacacatga
     2401 ccttttaagt tatgcgttga aaataaaaga gatgtttatt tgcttttgta tcgtcaataa
     2461 gcagcattaa actaacatat ttgaaactac acgtatgtta gtgaattaat cataagggag
     2521 tttttgtaat gaacaaattt aaagggaaca aagttgtatt aataggtaat ggtgcagtag
     2581 gttcaagcta cgcattttca ttagtgaacc aaagcattgt tgatgaatta gtcatcattg
     2641 atttagacgc tgaaaaagtt cgaggagatg ttatggattt aaaacatgcc acaccatatt
     2701 ctccaacaac agttcgtgtg aaagctggcg aatacagtga ttgtcatgat gcggatctag
     2761 ttgtcatctg tgctggtgct gcacaaaaac ctggagaaac acgtttagat ttagtatcta
     2821 aaaacttgaa aatattcaaa tcaattgttg gtgaagtaat ggcatcaaaa tttgatggta
     2881 ttttcttggt agctacaaat cctgttgata ttttagcgta tgcaacatgg aaattctctg
     2941 gtttacctaa agaacgtgtt ataggttctg gtacaatttt agactctgca cgctttagat
     3001 tattgttaag cgaagcgttc gatgttgcgc cacgtagcgt cgatgctcaa attattggtg
     3061 aacatggtga cactgaatta ccagtatggt cacacgctaa tattgcgggt caacctttga
     3121 agacattact tgaacaacgt cctgagggca aagcgcaaat tgaacaaatt tttgttcaaa
     3181 cacgtgatgc agcatatgac attattcaag ctaaaggtgc cacttattat ggtgttgcaa
     3241 tgggattagc tagaattact gaagcgattt tcagaaatga agatgctgta ttgactgtat
     3301 cagcattatt agaaggcgaa tatgatgaag aagatgttta tattggtgtt ccagcagtca
     3361 tcaatagaaa cggtattcgc aacgtcgtag aaatcccatt aaacgacgaa gaacaaagca
     3421 agttcgcaca ttcagctaaa acattaaaag atattatggc tgaagcagaa gaacttaaat
     3481 aactttttat aaaatctata ccattccaaa aattgtaaaa ccttacccca aaaaattgta
     3541 taaagggcta tttgatacaa ctatacgtgt cgagtagccc tatttttaat gtagtgaaag
     3601 tcgttgttga aattaaattt aaatctggca cttaagcttt aatcatagca ttaagatgat
     3661 tgtctagcag aggcgatttg cggactcact acagtgcatg atgaacttaa tgtttcaaat
     3721 gcaacattaa aaataaaagc agcgatgtca cttcttactc cgtacatcgt tgccattagt
     3781 tctgttgata tttcggttag tcttaatccc cgagcaattc ttcaatttca tttttgataa
     3841 ctgtaacgtg aggaccgtaa attacttgca caccagtgcc ttgctggatt acacctttgg
     3901 caccagtact ttcgagtagt actttatcga ctttgtcatt ttgatgaagt gtaacgcgta
     3961 gtctcgttgc acaacagtca acgatttcaa tgttatcctt gcctcccaaa ccagcaacaa
     4021 tagtttgtgc tctttcagta gcctcaactt gttgtgctgc agctttatct tcacgaccag
     4081 gtgttttgaa attaaatttc gtaattaaga atctgaaaac gatgtaatac aaacagaacc
     4141 acacaattcc aataggtatg acatataggt agtttgtttt actattacct tgtagcacac
     4201 caaaaagtaa gaaatcgata aagcctccac tgaaggtttg accaattgta atgttgaaaa
     4261 tgtctgccat cataaatgct aatccatcaa agaaggcatg gattacataa agaataggtg
     4321 cgacaaacaa gaaactaaac tctaaaggtt cggtaatacc tgttaaaaat gaagtgagtg
     4381 cagcggataa cattaaaccg ccgacaactt ttttatgttc aggtttagcc gtgtgataaa
     4441 ttgcaagtgc ggcaccacat aagccgaaca tcatcgtaat aaaacggcct gacataaagc
     4501 gtgacacacc tgaataatac ttcgtcacat ctggatcacc aagttgagca aagaagatgt
     4561 tctgcgtacc ttgaactaag tgacctttga cttctaaagt accaccaagt gccgtctgcc
     4621 aaaatggtaa gtaaaaaata tgatgtaaac cgagtggacc taacaatctt aagatgaagc
     4681 cataaacaaa agtaccgatg gcacctgttt tcgttacaaa tccacccaca tgataaatgc
     4741 cggcttgtat gcttggccaa atgaaaaaca tcaatacacc taaaaagatt gcggcaaatg
     4801 ctgtgacaat agggacaaat ctagagccac caaagaaacc taaatacggt ggtaatacca
     4861 ctttgtgata tttgttgtga agtattgcgg tcataatacc tgtgataatc ccgccaaaaa
     4921 caccggtttc aaccgtttgt ataccgagca ccatgccttg tccattttgt gcaagctgat
     4981 cttttgccaa tgtgcccgtg atagttaata agccattcat agttgcgttc ataattaaga
     5041 aaccgagcag tgcagctaaa cctgcagtac ctttatcgct tctagataat ccgattgcga
     5101 caccaattgc aaagatgact ggtaaatttt ggaaaacaat actacctgca gctgacatta
     5161 atgtaaaaat attttgtaat aaggtaatat ctaaaatagg gtatgcttta acggtgtttg
     5221 gattacttaa tgcaccaccg atacccaaca atagacctgc agctggtaag attgcgatag
     5281 gtagcataaa ggacttgccg aactgctgtg ctttttcaaa taaagatttc atcaacatcc
     5341 ctcctaatta ttctcaatat agcttttgag aaatttaata tcaatatata ttctgtgtat
     5401 gaaaatattt ttcataaaaa ttgtttgaat catgtaacaa tcatataaat tgctgtttat
     5461 attgtggtga aaatgagttg acaaaagtcg gtatagttct gtagacatcc tattttttag
     5521 cgaggtgggg tgacaaggca tatcggataa atttataagc cataaaatag ataaatagtg
     5581 tttgtatcca aaactatgtg aaagttaaaa ctatttttca aaataaacat gattcaccac
     5641 ataaataaat atacataagc tatcgataac aacttaagaa aggtggatat atataatgaa
     5701 aagaaagatt attatggatt gtgatccagg acacgatgat gcaatagcat taattttagc
     5761 gggggcaatt gacagtccac tagagatatt agctgtaacc acagtcgcag gtaatcaatc
     5821 agttgacaag aacacgacaa acgccttgaa cgtattagat attatgggac gccaagatat
     5881 agtagtagcg aaaggtgcgg ataggccgtt aattaaacta gttgcctttg cttctgaaat
     5941 acatggggaa tctggattag atggtccgaa actaccgtcg acaccatcac gtcaagcagt
     6001 tgcaatgcca gcatcagatg tgattattaa caaagtgatg acgagtgata cacctgtaac
     6061 aattgtagcg acaggtcctc ttacgaatgt agcaacggca ttgattcgtg agccaagaat
     6121 cgctgagcat attgaatcta ttactttgat gggtggtggt acatttggaa attggacgcc
     6181 tacagcagaa ttcaatattt gggtagatgc tgaagcagcg aagcgtgttt ttgaaagtgg
     6241 gattactata aatgtgtttg gtttagatgt aacacatcaa gttttagccg atgaacacgt
     6301 gattgaacgc tttgaaagta tcaataatcc tgttgcacag ttcgtcgtag aattattgca
     6361 attctttaag aagacataca agactcactt taatatggac ggtggtccaa tacatgatgc
     6421 ttgtacaatt ttgtatttgt tacaaccaga attgtttaca atggtacccg ttaatatcga
     6481 cattgaacat caaagtccac taacttatgg cactatggct gtagatttaa atcatgttac
     6541 aggtaagcct gccaatgcct attttgctac agcagttgat gttgaagaag tgtggaactt
     6601 gatagatcat aagttacgta catacgaata ataatactta attaaataga tacagttaac
     6661 cctaaggtgc ctgatttaag cgtccttagg gtttttgtat tggattacaa tggtcatggc
     6721 aatttgataa tgattgagat atgtatgtta aaaatgcttc aaaaaacaca aaatacaaaa
     6781 atatatgcaa aaatttcaat tgctttattt taaaagatta aaatcacaaa aaataccgat
     6841 ataattggaa tgttgtcgaa acatcacaat gatgtacata ctcattttga aaatatgtgt
     6901 tcaacgagtg tacatcggtg tgacgattga tgataaattg agctcagatg ctcaaaggag
     6961 gatcgaacat gaacggggat aatcagcaaa tactcagaga aattgtattg aatcctacta
     7021 ttcatggtaa agaacttgaa tcgatatttg gtttgtctcg tagacaacta ggatatcgca
     7081 ttcaaaaaat caatttgtgg cttgaacaag agggttatcc aaaacttgaa agaacaagcc
     7141 aaggaaattt tattgtaagt tctgaaatca tgacgttatt caaacgagat gtatcggagc
     7201 agcaaatgtt aaacggcaac aatgtcattt ttagcataga aacacgtcgt tattatttaa
     7261 tgctcatgct ttttagtaag gaaaacgcaa tgtctctaaa ccatttttca attgatttac
     7321 aagtcagtaa aaatactgtc attcacgata taaatcatgt gaaagagcaa ttggaagatc
     7381 atggtttgtc acttaagtat tctcgaaaac atggttatga aattgttggt gatgaatttg
     7441 aagttcgccg tttcttcatt aagttgattg atcaaaggtt aaatcatgat attactaaaa
     7501 gtgaagtttt aaaggcgctc aacttaacat tcgaagatat cgcatatcaa aaagacaaga
     7561 tcaaacaggt agaacaattt ttgaagagtc gctttataga caaatcactg agttcattgc
     7621 cttacgtcct ttgtgtgatt cgtagacgaa ttcaaagtgg tcatgtgatt aatccattaa
     7681 atattaatta tcagtatttg agggatacga aagaatatca agcaacggag attatgacgc
     7741 aacatgagcc ggatttgcca gaagcggaaa agttatattt gacgctacac ttactttcaa
     7801 caagtgtgca atggactgat ttgcaggaat cagataacat atcgaattta acgatggcca
     7861 ttgatcaaat gattcaccat tttgaacaaa ttacttttat taacattgaa gataaggaga
     7921 aattatcaca gcaactcttg ttacatttaa cgcctgcttt ttataggatt aaatataact
     7981 taacggatcg tgatgaatta ataaatcctt tacaaggaaa ttatcaatcc ttatttcata
     8041 tggtgaaaca atcatgtcaa tcgttaactg aatatttcgg aaaatcattg cctgataatg
     8101 aaatagcata tttaaccatg ttgtttggag gtagtttgag acgtcaagat gaaaacttcg
     8161 atggcaagat aaaagctatt atcgtgtgta cacaaggtac gtcagtatca caaatgatgt
     8221 tatacgagtt gcgaaactta tttccagaaa ttattttctt agatgcgatt tcacttagaa
     8281 catttgaaaa ttacacatta gattatgaca tcgtcttttc accaatgttt gtcttaacac
     8341 ataaaaaatt atttatcaca aaagtagctt tatctgaaaa tgagcaatga aagttacgta
     8401 aagaagtgat gaagtacatt aataaggaat cggctgacat tgataaggaa ataaacaagt
     8461 taatggcatt aattgaacgc actacgacag ttaatgacat tacagaacta cgtgatggtt
     8521 tagaagattt tattgcgaat tataattcaa tttcaaccat taatggatcg attgtcacac
     8581 aaaataagac attagattta gctgacttga taccagcaag gcacgtgaaa agaatacatc
     8641 atgttgaaaa tattgatgaa gctattgcta aagcaagtga tgtgttagtt gctaatcatt
     8701 ttattgatat taaatatatt catgagatgc aacaggcatt tgatgattcg tatatggtta
     8761 tcatgcaaaa tattgctatt ccacatgcat actctgaaaa gcatgtacat aaaacagcga
     8821 tgagtatgtt gatattacaa gaaccaatat acatgtcaga tggcacagca atccatatta
     8881 ttgtacctat tgctgctgtt gataaagtga cacacttaag agcgttacta caattgagag
     8941 atgtggcgca aaacaatgac gcaattaagc gcatcataca aagtcgcaaa aattctgatg
     9001 taaatgagat tttaaaaaat tattcaaata aagaagcgag ggaaaatgga tgggacagca
     9061 attagtgcat aaagaaaata taatgctcaa tttgtcggca actgataaag aatctgtatt
     9121 gtcacaaatg tcagatgtgt tatttcaaaa tgggttcgta aagtcaacgt ttaaagatgc
     9181 agtcatcgac agagaaaaag agtttgctac tggtttacca acgcatctat gttcggttgc
     9241 tataccgcat acagatgtcg aacatattaa ccatagaacg ataggtgtgg ctgttctaga
     9301 aaaagaagtg ccgtttattg aaatgggaac acttgatcaa cagacagaag tgaaaatcgt
     9361 ttttatgtta gcaatggata aagtagatga tcaacttaag ctgttacaac agttgatgca
     9421 aatttttcaa agtgaagaaa aattggagca gattctacga acgaaagatg aaacaatttt
     9481 agcaacacta atcaatgatt atttgaaata taactaaaaa ttaattggag gaattgaaaa
     9541 tgaaacaagt attagtagcg tgtggtgcag gtattgcaac gtcaacagta gtaaataatg
     9601 caattgagga aatggcaaag gaacacaata ttaaagtaga tattaaacaa atcaaaatta
     9661 cagaagttgg accttatgaa gacactgcag atttattagt tacaactgca atgacaaaga
     9721 aagaatataa attcccagtt atcaacgcac gtaatttctt aactggtatt ggtattgaag
     9781 aaacaaaaca acaaatctta acagagttac aaaaataact gaattgatat gtaacgtggg
     9841 tcaataacat ataaatatat gcaaattgca tataaatgac tatcacgaca aatgattgta
     9901 gatgacatag aaacgcagtg actgtaattc aaagggattg cagtcgaatg tagcaagggt
     9961 tgtcgttgaa atgactgtaa cgtcaaatgt aatcacaatc ggaaagtatc gtgacaatgc
    10021 atatataaca gggagggttt aaatatgagt tacttcactg attttgtaag gggattttta
    10081 gatttaggtg caactgttat tttaccggtt gtcatattct tgcttggcct attctttagg
    10141 cagaaaattg gagcggcatt taggtctggt ttaacaatag gtgtggcttt tgtagggatt
    10201 ttcttagtca tcgatttatt agttaaaaat ttagggccag cagcacaagc gatggttaaa
    10261 aatttaggcg tcagtctgaa tgtgattgat gtaggttggc cagcaacatc atctatcgcg
    10321 tgggcatcat ctgtcgcagc atttattatt ccactcggaa tcattgttaa cgttgtattg
    10381 ctagtaacta aagtgacaaa gacgatgaat gtagatattt ggaatttttg gcattatacg
    10441 tttacagcag caatggttta tgccgtatca ggcagtattt ggcaagcgtt attagcagca
    10501 gttattttcc aagttatctg tttgaaagta gcagattgga cagcaccgat gatgagtgag
    10561 ttctttgatt taccaggtgt atcgattgct acaggaagta caatttctta tgcaccaggt
    10621 atttacttag ttaaattgtt acaaagagta cccggtctga ataagttaga tgctgatcct
    10681 gaaacaattc aaaaacgttt tggcgcattt ggagagtcta tctttgtcgg attaatttta
    10741 ggtttaggta ttggtgtgtt agcaggttac aaacctggag acatcattaa tttaggaatg
    10801 tcaatggctg cagtaatggt attaatgcct agaatggtaa aaatcttaat ggaaggttta
    10861 atgccagttt cagagtctgc aagaacatgg ctaaataaac gttttggcga acgtgaaatt
    10921 tatattggat tggatgcggc tgtagcatta ggtcatccag cggttatttc gacagcatta
    10981 attttagtac ctatcactgt tttattagcc gttattttac caggaaacca agtattacct
    11041 tttggtgact tagcaactat accatttgtt gtcgcgttta ttgttggtgc agcaagagga
    11101 aacattattc attctgtcat tgtgggtacg attatgattg caatttcact atatattgca
    11161 acagacgtag cacctatttt cacagatatg gcgaaaggta cgaatgtaca aatgccaaaa
    11221 ggttcatctg aaatttcaag tattgatcaa ggtggtaata tcgttaacta tcttatcttt
    11281 aaactattta gtctattcaa ttaaaaatcg aggtgtttgt ggtgaaagct ttagtaaaaa
    11341 caagagaagg acatggcaac ttagaacttc ttgataaaga agttgcaaca ccgctagatg
    11401 ataaagtaaa gattaaagta cattatgcag gaatttgtgg cacagatatt catacttatg
    11461 aaggtcatta taaagttaat tttccagtga cattaggtca tgaattttct ggtgaaatcg
    11521 ttgaagttgg agcagacgtt aaagatttta aagttggtga ccgtgtcact tctgaaacga
    11581 cattctatgt ttgtaatgag tgtgaatact gtgaatcaaa agactataat ttatgcaacc
    11641 atcgaaaagg tattggaaca caagttgatg gcgcatttac taattatgtc attgcacgtg
    11701 aagaaagttt gcatcatatt ccagacgaag tgtcgtatca gtctgcagct atgacagaac
    11761 cattagcatg tgcacatcat ggcgtttcta agattcaagt caattcaggc gatgtagcag
    11821 ttgtaatggg acctgggcca atcggattac ttgtagcaca agtgttgaaa agtaaaggcg
    11881 caactgttgt ggtaactgga ttggacaatg acaaagtcag attagataaa gcagaagcat
    11941 tgcacatgga ttatgtagtc aatttacaac aaacagactt aaaaacgtat atcaatggaa
    12001 ttacagacgg atacggtgca gatgttgttg ttgaatgttc aggtgcagtt ccagcagcac
    12061 gacaaggttt ggatatttta cgcaaaaaag gtttctacag tcaaataggt atttttaagg
    12121 atgctgaaat tacatttgat atggaaaaag tgattcaaaa agaaataaca gttgttggta
    12181 gtagaagtca aaagccggca gattgggaac cttcattgca acttatggcg gatggtttag
    12241 taaatgctga agctttggtg acaaaaatat atgatatttc gaaatgggac gaggcgtatc
    12301 aacatttaaa atctggcgaa ggtattaaag cattacttaa gccgctcgat ttagatgaaa
    12361 atgaaggaga gaattaatat ggtagaatca atgctaactt ttatgcttgg gccattgaga
    12421 caaatcactg atttttatat ggaacatcta cttgtaagta attctatcgt cattgcaggt
    12481 tattttgcga caggtatttt taaaaagaaa aaagttgtga attaaatcaa agttgaggtg
    12541 atttacaagt gaaagctttg aaattatatg gcgtggaaga tttacggtat gaggataatg
    12601 aaaagccagt cattgaaagt gcgaatgacg ttattgttaa agtacgagcg actggaatat
    12661 gtggttcaga cacgtcacga tacaaaaaaa tggggccata cattcaaggt atgccatttg
    12721 gtcatgaatt ttcaggtgtt gtagatgcca ttggaagtga tgttacgcat gttaatgtag
    12781 gcgacaaagt gacaggttgc ccagccatac cttgttatca atgcgagtat tgtttaaaag
    12841 gtgaatatgc acgatgtgaa aagttgttcg tcattggctc atatgaacct ggatcgttcg
    12901 cggaatatgt caaattgcca gcgcaaaatg ttttaaaggt accagacaat gttgattaca
    12961 ttgaagcagc aatggttgag ccatcagccg ttgttgcgca tgggttttat aaatcgaata
    13021 tacaacctgg tatgactgtt gcagtaatgg ggtgtggcag tataggattg ttagctattc
    13081 aatgggcacg aatatttggt gctgcgcata tcatcgctat agatatagat gcgcataaac
    13141 tagatattgc tacatcattg ggcgcacatc aaaccatcaa ttcaaaagaa gaaaatcttg
    13201 agaaattcat cgaaaatcat tacgccaatc aaatcgatat agctatagaa tcatcaggtg
    13261 ctaaagttac gattggtcaa atattgacgc tacctaaaaa aggtggcgag gtggtattac
    13321 tcggaatacc atatgatgat attgagattg atcgcgttca ttttgaaaaa attctgcgta
    13381 acgagttgac agtatgtggc tcttggaact gtttgtccag taattttccg gggaaagagt
    13441 ggacggcaac cttacattat atgaagacga aagatattaa tgtaaagcct attatttctc
    13501 attttttacc gttagaaaaa ggcccggaga catttgataa attagttaac aagaaagaac
    13561 gatttgataa agtcatgttt acgatttatt agtatgcacc tttgaggacg aaaacgctgg
    13621 tatagttata gctatgaaag tgcgaatgcc gtctggtcta cagatactat cgaaataatt
    13681 catcttcgaa tatacgttgc taaatagccg gtttacttgt gtgaaatatg cttgtgaatc
    13741 ggttgttttg cattttgtat acttaaaatg agatggcaat atttgataat ttttaaagtg
    13801 aaaatcaagt acagccactt aataagataa atttattata atatatggta aaatgatggc
    13861 agtaataatg aatttgaaaa agagtaaa
//
LOCUS       DATTQF010000063        13726 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.063, whole genome shotgun sequence.
ACCESSION   DATTQF010000063 DATTQF010000000
VERSION     DATTQF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.063"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(42..227)
                     /locus_tag="VB241_001525"
     CDS             complement(42..227)
                     /locus_tag="VB241_001525"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP1244820.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            375..1637
                     /locus_tag="VB241_001526"
     CDS             375..1637
                     /locus_tag="VB241_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEP1244821.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHNQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYS
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(1773..2864)
                     /locus_tag="VB241_001527"
     CDS             complement(1773..2864)
                     /locus_tag="VB241_001527"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEP1244822.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIHMPTKPGVIVSDTGSTKAMI
                     QQHECSLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNSQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAEA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            3029..4060
                     /locus_tag="VB241_001528"
     CDS             3029..4060
                     /locus_tag="VB241_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEP1244823.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILKLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDSSGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            4553..5959
                     /locus_tag="VB241_001529"
     CDS             4553..5959
                     /locus_tag="VB241_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEP1244824.1"
                     /translation="MDVFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSIVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKPIPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHANQHLNQLTFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGEINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            5956..6522
                     /locus_tag="VB241_001530"
     CDS             5956..6522
                     /locus_tag="VB241_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP1244825.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIIQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQHLMKIISSYQDKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDVPEQFSIMRYHSLISNPDNFPEELKITGRTDDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINYVKKG"
     gene            6528..7524
                     /gene="trpD"
                     /locus_tag="VB241_001531"
                     /pseudo
     CDS             6528..7524
                     /gene="trpD"
                     /locus_tag="VB241_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
     gene            7526..8308
                     /gene="trpC"
                     /locus_tag="VB241_001532"
     CDS             7526..8308
                     /gene="trpC"
                     /locus_tag="VB241_001532"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEP1244826.1"
                     /translation="MTILEEIVEYKQLLLQNGYYQDKLNTLKSVNIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHYYISESGIH
                     DVSDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            8308..8940
                     /locus_tag="VB241_001533"
     CDS             8308..8940
                     /locus_tag="VB241_001533"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEP1244827.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPDHIDKVCVVVNPDLTTIEHVLSNTPINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKNIKDIPYLIAGG
                     INTENIQTVNQFKLSHQGFDLASGIEVNGRKDIEKMTAIVNIVKGDRENE"
     gene            8933..10147
                     /gene="trpB"
                     /locus_tag="VB241_001534"
     CDS             8933..10147
                     /gene="trpB"
                     /locus_tag="VB241_001534"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEP1244828.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            10140..10868
                     /locus_tag="VB241_001535"
     CDS             10140..10868
                     /locus_tag="VB241_001535"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEP1244829.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFEQLEKHGNQIKCQYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPFELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSYAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRSPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            11190..12452
                     /gene="femA"
                     /locus_tag="VB241_001536"
     CDS             11190..12452
                     /gene="femA"
                     /locus_tag="VB241_001536"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HEP1244830.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKERVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            12471..>13726
                     /gene="femB"
                     /locus_tag="VB241_001537"
     CDS             12471..>13726
                     /gene="femB"
                     /locus_tag="VB241_001537"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HEP1244831.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLK"
BASE COUNT         5001 a         1969 c         2544 g         4212 t
ORIGIN      
        1 ttcgttttca aagaattaat tggcatgcat cttatgaaga attattgatc tgactttctt
       61 acgccagcca caccataatg gtttggtttc atttcttcta taacaacgtg aattgcttgt
      121 ctatttgctc ccgttgtttt ttctacggcg tcagtaactt cgctaactaa attttttaat
      181 tgttcatccg aacgaccttc taataatttt acattgacga ttggcatcat ccaaacctcc
      241 taatcattat taatttgagt atatcatgta atacttaaaa attcatttgt aaaataacag
      301 aacatttgtt ctaaaatggt tgaaatagaa caagtgttcg tatataatgg aattcaagta
      361 agggaggaat aacagtgtat aattatcatt tattagaaga tagggatgtt ctatgtattg
      421 atcaaaaaag tttttttgcg agtgtttctt gtattgaaaa ggggctagat ccattagaaa
      481 caaagttagc tgttgttgca gatactaagc gtcagggttc tgtagtattg gctgcgacac
      541 ctaaattaaa agaattaggc atcaagacag gatcgcgatt gtttgaaata ccacatagaa
      601 atgatattta cattatcaat ccaagtatgc gtaaatatct taatgtttca gttgctattt
      661 ctaagattgc attgcgttat attccacctg aagatttaca ccaatatagt attgacgaat
      721 tttttatgga tgttactgat agctatcata gatttagttc tacagtacat gcattttgcg
      781 aaagacttaa acgtgaaatt tatgaagaaa caggcattta ttgtactgtg ggcattggtt
      841 ctaatatgtt attaagtaaa attgctatgg atgttgaagc taagcataat caaaatggta
      901 tagctgaatg gcgatatcaa gatgtaccaa caaaattatg gccaattcag cccttgagag
      961 atttttgggg tattaatcgt cgaacagaag ccaaattgaa taaaagaggg atttttacta
     1021 taggagattt agcaaaatat ccatataaat ttttaaaaaa agagttcggt attttaggtg
     1081 ttgatatgca tctacatgcg aatgggatag atcagagtaa agtacgtgaa aagcataaga
     1141 ttagcaatcc atcgatatgc aaaagtcaaa tattaatgag agattatcat tttgacgaag
     1201 caaaagtagt aatgcaagag ttaattgaag atgttgctag cagagttcga gcaagaaaaa
     1261 aagtggcaag aacgatacat tttgcctttg gctatagtga tgaaggtggt gtacataagc
     1321 aatatacttt gaaagatcca acaaacctag aaaaagatat ttataaagta gtaatgcatt
     1381 tcgcagataa attatgtaat aaacaagcac tatatcgtac gctaagtata tctttgagtc
     1441 aatttattaa tgaggatgag cgacagttaa gtctgtttga agatgaatac caacgcaaac
     1501 gtgacgaatg tctagctaaa acgatagacc aattacattt gaaatacagc aaaggtattg
     1561 tgtccaaagc agtatcgttt acagaagcag gtacaaaaca tggtagatta ggtttaatgg
     1621 ctggacataa gatgtaatga ctatacggtt taaggaatat ataactgtga ttcgtataaa
     1681 ataagtctct aaagataaat atttcatata tcacaataga tttcacaata atatctaaga
     1741 atacatggaa tttatcaaaa gagactcaat aattattgga tataacaatc aaaatcactc
     1801 aatgcttgca taccgcgttc tcgatcggta ggatttttga aactaatttt taaagcaccg
     1861 tatatatctt cgcgtacttc taagattctt aagttgctta tagatatatt atgtaaactt
     1921 aggatataag tcactttgct tatcatacct gattcatccg gaatatctac atatagatca
     1981 tacgcagtat ttagtccacc tagttgttta gcgggcagtg cgtcacgata cgatttagct
     2041 tcggcaaaaa atgataacaa tttttcagaa tcattgcttt caattagtct ttctaaatct
     2101 tgaaactgac tttttagctg tcgaatcatt tctaaaatat acgttttatt actcaaggtg
     2161 atatctttcc acatttgtgc attactacta gctatacgag tgatatcacg aaaaccacca
     2221 gctgcaagtt tattgactaa atgatgttct tgactgttct tttgactaac atgaactaaa
     2281 ctagatgcaa cgatatgagg taaatgactt acgacgcttg ttacgtagtc gtgttcttca
     2341 gcagtagtta caataaattt agcaagagta ggtgataaca gttcttttaa cgtgtttgct
     2401 gcttgctcat ttcttggctc attgtagact aaaatataat aagcgttttc aaataagtgc
     2461 tttttagcat ttagtacacc ggatttatga ctaccagcca ttggatgacc actgactaaa
     2521 tgaatattat gctttaataa actgcattcg tgttgctgta tcattgcttt agtactacca
     2581 gtatcagaaa caataacacc aggtttagtt ggcatatgta taagctcgct aagatattta
     2641 tttgtgatag caacaggtgt tgcataaata attacatcgg cttttttaat agcttcacta
     2701 taatttaaac atttttcatt aataataccg attgatttag ctttatctaa ctgagaagta
     2761 tctgcatcgt atgcaataat attagtatta gggttatggt attttatatt gctagcaaga
     2821 cttccaccaa ttaatccaag cccaacaaat aaaactgttg tcatataaat caccttattt
     2881 cgaaattttc agaataataa cattgtaaat gagctgttga cacagtgcaa tagtaaataa
     2941 aaatcgataa tagcattaat agataaacgg atataaatca tctacaataa agagtatagt
     3001 aacacaatgg caacggaggg gtaaatcaat ggaaccaata ttagaaatga ttaaaacatt
     3061 aacaggtatt aatagtcctt caggagacac agaagaagca attcaatttg tcgaaaaata
     3121 tgcaaaagac ttgggttatc aaacaacact aacaaataaa ggtgcgttat taataacagt
     3181 gccaggcaaa aatgatgaag tacaacgctg tattactgct catgttgata ctttaggtgc
     3241 aatggttaaa gaaattaaag aagatgggcg cttagcaata gaattaattg gaggattcac
     3301 gtataacgcg attgagggtg aatattgcca aattaaaact gatgctggtc aaatatatac
     3361 aggaaccatt tgtctgcatg aaacaagtgt tcatgtatat agaaataatc atgaaatacc
     3421 tagagaccaa aagcatatgg aaataagaat tgatgaagta actacatcag aagaagatac
     3481 aaagagttta ggtatttcag taggtgattt tgttagcttt gatccacgta cagttatcac
     3541 gtcatcaggt tttattaaat ctcgtcattt agatgataaa gctagcgtag cgatgatact
     3601 aaaattacta aagaaattaa aagaagagca aataatatta ccacatacaa cgcaatttta
     3661 tatttctaat aacgaagaaa taggttacgg tgcaaatgca tcaattgatt cgaaaatcaa
     3721 agaatatatt gcattagata tgggcgcgtt gggagacggt caagcatccg atgaatatac
     3781 agtttctatt tgtgccaaag attcttcagg tccatatcat aagcaattga aatcacacct
     3841 agttaatctt tgcaaaataa ataatattcc atataaagta gacatatatc catattatgg
     3901 ttcagatgct tcagcagctt tacatgctgg tgcggatatc agacatggtt tatttggcgc
     3961 tggcattgaa tcatctcatg caatggaacg aacacatatt gattctatta aagcgacaga
     4021 gaaattacta tatgcatatt gcttatcacc aattgagtaa acaattagtg ttgacaaatg
     4081 tgaacgacct atgtaatata atgaactata aaaataatta gaattttcta aagaaatagt
     4141 agcagatatg aaacgtagca aatagaaagc taatgggtga tgggaattag cacgccatat
     4201 cttgtgaatt ggactttgga aaacaattga atgagttttg aaagtgaaca tgaattatgt
     4261 taactaaggt ggcaccacgg taacgcgtcc ttacaggtat atgcgttatg tggtgtcttt
     4321 ttatttagac aaaatgtagt agttagttaa aggtagcaac agaaagttag tgggtgatgt
     4381 gaactaacac cgagattaat gaaattgggt tttgtctgca acagaaaaat tatatatagt
     4441 aaagagtgaa ctatgaatat ttcgaatatt cggttaattt aggtggtacc acgcgtcagc
     4501 gtcctttata ttgataagga tgctgacgtt tttttagaaa ggagcgtaaa gaatggatgt
     4561 attctataaa aaaataaaag caaatgtaac acctgaagtt ttagcacaac ttcattccaa
     4621 gaagatcatt ttggaaagta caaatcaaca acaaactaag ggtcgctatt ctatagtgat
     4681 atttgatatc tacggtactt taactttaga taacgatgta ttatcagtaa gcactttaaa
     4741 agagtcgtat caaatcactg aaagaccgta tcattattta acgactaaaa taaatgaaga
     4801 ctaccataat attcaagatg agcaacttaa gccaataccg tttatatctg gatatgttgg
     4861 gacgtgtagc tttgatttag taagacatga atttcctaaa ttgcaatcaa tacaattaga
     4921 ggatcacaag caacacgatg tacggttata tatggttgaa caagtttatg tatttgacca
     4981 ttacaaagat gagttatata tcatcgcgac gaatcaattt tcaaattcaa cgaaatcaga
     5041 tcttgagaat cgagttaata agtctatcga agacttaact aaaatccaac cattcatgcc
     5101 tacacaagat tttgatttta aaactaaaga aattcaatca aatatttctg aagaaagatt
     5161 tatcgaaatg attcagtatt tcaaagagaa aataacagaa ggggatatgt tccaagttgt
     5221 gccatcaaga atttacaaat atgcgcatca tgctaatcag catttaaatc aacttacgtt
     5281 tcaactgtat caaaatttaa aacgacaaaa cccaagtcca tatatgtatt atcttaatat
     5341 cgatcaaccc tacattgtcg gtagttctcc cgaaagtttc gtaagtgtca aagatcaaat
     5401 tgtaacaact aatcctattg caggtacgat tcaacgtggt gagacgacac aaatagataa
     5461 tgagaatatg aaacaactac ttaatgatcc taaagaatgc agtgaacatc gtatgcttgt
     5521 tgatttaggg cgtaatgata ttcatagagt aagtaaaata ggtacctcaa aaattactaa
     5581 attaatggtt attgaaaaat atgaacatgt tatgcatatc gtaagtgaag tcacaggtga
     5641 aataaatcaa aatttatcgc caatgacagt tattgcgaat ttattaccaa caggtaccgt
     5701 ttcaggtgca ccaaaattac gtgcaatcga aagaatttac gaacaatatc cacataaacg
     5761 tggcgtttat agtggtggtg ttggatacat aaattgtaat cataacttag attttgcatt
     5821 agcaattcga acgatgatga tagatgagca gtatatcaac gtagaagctg gttgtggggt
     5881 tgtatatgat tctattcctg aaaaagaact aaatgaaacg aaattgaaag ccaaaagttt
     5941 attggaggta agcccatgat tttagttgta gataattatg attcctttac gtataaccta
     6001 gtggatattg tggcacaaca cactgatgtc attattcaat accctgatga tgataatgtg
     6061 ctgaatcaat ctgttgatgc tgtcattatt tcaccaggcc ctgggcatcc attagacgac
     6121 caacatttaa tgaaaattat ctcatcctat caagataaac ccattttagg tatttgccta
     6181 ggtgctcaag cactgacttg ttattacggt ggagaagtca ttaaaggcga caaagttatg
     6241 cacggaaaag ttgatacgct taaggttata tcgcatcatc aacatctgtt atatcaagat
     6301 gtaccagaac aattttctat tatgagatac cattcattaa taagtaatcc tgataatttt
     6361 ccagaagaat tgaaaattac tggacgtact gatgattgta tacaatcatt cgagcataaa
     6421 gaaagaccgc attatggtat tcagtaccat cctgaatcat ttgctacaga ctatggtgtc
     6481 aaaataatta caaattttat taattacgtg aagaaaggat gaaaaccatg acattactaa
     6541 caagaatcca aactgaatct atattgcttg aaagtgacat taaggagtta atcgacatac
     6601 ttatttcccc aagtatcgga actgatataa aatatgaatt actgagttcg tattcggaac
     6661 gagaaattca acaacaagaa ttgacatata ttgtacgtag tttaattaat acaatgtacc
     6721 cacatcagcc ttgttatgag ggcggtatgt gtgtgtggta caggtggtga caagtctaat
     6781 agcttcaata tatcaactac tgtcgctttt gttgtagcaa gtgctggcgt aaaagttata
     6841 aaacatggaa ataaaagtgt gacttcaaat tcaggtagta cggatttgtt aaatcaaatg
     6901 gacattcaaa ctacgtctgt tgatgatacg ccaaagcaat tagatgaaaa aaacctcgtg
     6961 tttatcggcg caacagaatc gtatccaata atgaaatata tgcaaccagt tagaaaaatg
     7021 ataggtaagc ctacaatatt aaaccttgta ggtccattaa ttaatcccta tcacctaacg
     7081 tatcaaatgg taggtatctt tgaccctaca aaactaaaac tagttgcaaa gacaattaaa
     7141 gatttaggta gacgacgtgc aatcgtatta catggtgcga atggtatgga tgaagcaacg
     7201 ttgtctggtg ataatttgat atatgaattg actgaagatg gagaaatcaa aaattacaca
     7261 ttaaatgcga ctgattatgg tttgaaatat gcacagaata gtgattttaa aggtggttcg
     7321 cctgaagaaa acttagcaat cacacttaat atcttgaatg gtaaagatca gtcaagtcgc
     7381 cgtgatgttg tcatacttaa tgcaggttta agtctatatg ttgcagagaa agtggatagc
     7441 atcgcagaag gcatagaact cgcaagcact ttgatagata atggtgaagc tttgaaaaaa
     7501 taccatcaaa tgagaggtga ataacatgac aattttagaa gaaattgttg aatataaaca
     7561 gttactttta caaaatggct attatcaaga caaacttaat accttgaaaa gtgtgaacat
     7621 tcagaataaa aaatctttta taaatgcaat tgagaaagaa ccaaagctag caattattgc
     7681 agaaattaaa tcgaagagtc ctacagttaa tgacttacct gaacgagatt tatcgcaaca
     7741 aatctcagat tatgagcaat atggtgcaaa tgctgtatcc attttgactg atgaaaagta
     7801 ctttggtggt agttttgaaa gattacaagc attgacaaca aaaacaacat tacccgtatt
     7861 atgcaaagat tttattatag atccgcttca aattgatgtt gccaaacaag ctggtgcatc
     7921 tatgatttta ctgattgtta atatcttatc ggataaacaa ttgaaagatt tatataacta
     7981 cgctatatcg caaaatttag aagtgttagt tgaagtacat gatcgtcatg aattagaacg
     8041 tgcctataag gttaatgcta aattgattgg tgtaaataac agggacttaa aacgatttgt
     8101 cacaaatgtg gaacatacaa atactatttt agaaaataaa aaaccaaatc attattatat
     8161 ttctgaaagt ggaattcacg atgtatctga tgtaagaaaa atcttgcata gtggtatcga
     8221 tggcttacta ataggtgagg cgcttatgcg ttgtgacaat ctatctgaat ttttaccaca
     8281 actgaaaatg caaaaggtga agtcatgatg aaattgaaat tttgtggctt tacatcaatt
     8341 aaggatgtta cagcggccag tcaattacct attgatgcga taggtttcat ccattatgaa
     8401 aaaagtaaaa ggcatcaaac aattacccaa ataaaaaagt tagcgtctgc tgttccagat
     8461 catattgaca aagtatgtgt cgtggtaaat cctgatttaa caactattga acacgtatta
     8521 agcaatacgc caattaacac aatacagtta cacggcacag aatctattga ttttatacag
     8581 gaaataaaaa agaagtattc aagcattaaa attactaaag ctttagctgc agatgaaaac
     8641 ataatccaaa acataaataa atataaaggg ttcgtagatt tatttattat cgacaccccc
     8701 tcagtgtcgt atggtggtac cggtcaaaca tatgactgga ctattttgaa gaacataaaa
     8761 gacatacctt acttgatagc aggaggcatt aacactgaaa atattcaaac agttaatcaa
     8821 tttaaattat cacatcaagg ttttgatctt gcatcaggta tagaagtaaa tgggcgaaaa
     8881 gatatagaaa aaatgacagc aattgtaaat attgtgaaag gagatagaga aaatgaataa
     8941 acaaatacaa acagaagcag atgaattagg tttctttggt gaatacggag ggcaatatgt
     9001 tccagaaaca ttaatgccag caattattga gttgaaaaaa gcttataaag aggcaaaagc
     9061 agacccagag tttcaaagag aacttgaata ctatttatca gagtatgtag gacgcgcgac
     9121 gccacttaca tatgctgcat cgtatactga aagcttaggt ggcgctaaga tatatttaaa
     9181 acgagaggat ctaaatcata caggcgccca taaaattaat aatgcgttag gtcaagcgtt
     9241 gctcgctaaa agaatgggca agaagaagct tgttgctgaa acaggtgcgg gtcaacatgg
     9301 tgtagctagt gctacggttg ctgcattatt tgatatggaa cttgttgtct ttatgggaag
     9361 tgaagatatt aaaagacaac aacttaatgt atttagaatg gaattacttg gtgcaaaggt
     9421 tgtggcagtt gaagatggtc aagggacttt atcggatgca gttaataaag cattgcaata
     9481 ttgggtaagt catgtagatg atacgcatta tttattaggt tctgcattgg gtccagaccc
     9541 attcccaacg attgttagag attttcagag tgtgattggt aaagaaataa aatcacagat
     9601 attgaagaaa gaaggtcgac ttccggatgc aattgtagca tgtatcggtg gtggctcaaa
     9661 tgcaatcggt acattttatc catttattaa agatgatgtt gctttatacg gtgttgaagc
     9721 cgcaggtcaa ggcgaggata ctgataaaca tgcacttgca attggcaaag gatcacctgg
     9781 cgtattacat ggtactaaaa tgtatttaat tcaagatgaa ggtgggcaag tgcaactagc
     9841 acattctatt tcagcaggac ttgattatcc tggtattggg ccagaacatt cttattacca
     9901 cgacattggt agagtaactt ttgaaaatgc aagtgataca caagcaatga atgctttaat
     9961 caactttaca aaacatgaag gtattatacc tgcaattgaa agtgcacatg cactgagtta
    10021 tgttgaaaga ctagcgccta cgatgtcgaa agaagatatt attgtagtaa caatttctgg
    10081 acgtggcgat aaagatatgg aaacaattag acaatatatg gcagagcgag gtcttgcaaa
    10141 tgactaaatt atttatacct tatattatgg gcaataaaga tttgattgaa aatgcaacat
    10201 tgttgagtga aaatggtgca gatataattg aaattggtgt acctttctct gatccggttg
    10261 ctgatggtcc agttatcatg gaagcaggtc aacaagcgat taaacaaggc ataacgatag
    10321 attatatttt cgagcaatta gaaaaacacg gtaatcaaat taagtgtcag tatgtattaa
    10381 tgacgtatta taatataatt tgtcattatg gagaacaagc gttttttgaa aaatgtcgag
    10441 ataccggtgt ctacggctta attattcctg atttaccatt tgaattatcg cagcgtttaa
    10501 aacaacaatt tagtcactat ggtgtcaaaa tcatatcgtt agttgcgatg actactgatg
    10561 acaaacgtat aaaagatatc gtatcatatg cggaaggctt tatttatact gtgacaatga
    10621 atgcgacgac aggacaaaac ggtgcgtttc acccagaatt aaaacgaaaa attgagtcaa
    10681 ttaaagcgat agccaatgtg ccagttgtcg caggatttgg tataagatct ccacaacatg
    10741 ttgcagatat aaaagaggtt gcagatggta tcgttattgg tagtgaaata gtaaaacgat
    10801 ttaaatctaa cacgcgtgaa gaaatcatta aatatttaca atctatccag caaacattga
    10861 ataattaagt ttacttgatt taaaaaaatt agtcgaatac tgtttgaaaa agtgaaaaac
    10921 ggtgaattat aaaattgaat agaaatgata caaaagtaat atgagcaaac ccaaacgttc
    10981 atattacttt ttttgaaatt gtatgcaaaa atctaaatat tactataaaa gtatacgcaa
    11041 ttaaagcgtt tatgttttag ttttaacatt aactattgta tacttattta gattagattt
    11101 attatttttg acatttgcag aggggaaata gaaaaactgc aaatacggaa atgaaattaa
    11161 ttaacgagag acaaatagga gtaatgataa tgaagtttac aaatttaaca gctaaagagt
    11221 ttggtgcctt tacagatagc atgccataca gtcatttcac gcaaactgtt ggccactatg
    11281 agttaaagct tgctgaaggt tatgaaacac atttagtggg aataaaaaac aataataacg
    11341 aggttattgc agcttgctta cttactgctg tacctgttat gaaagtgttc aagtattttt
    11401 attcaaatcg cggtccagtg atcgattatg aaaatcaaga actcgtacac tttttcttta
    11461 atgaattatc aaaatatgtt aaaaaacatc gttgtctata cctacatatc gatccatatt
    11521 taccatatca atacttgaat catgatggcg agattacagg taatgctggt aatgattggt
    11581 tctttgataa aatgagtaac ttaggatttg aacatactgg attccataaa ggatttgatc
    11641 ctgtgctaca aattcgttat cactcagtgt tagatttaaa agataaaaca gcagatgaca
    11701 tcattaaaaa tatggatgga cttagaaaaa gaaacacgaa aaaagttaaa aagaatggtg
    11761 ttaaagtaag atatttatct gaagaagaac taccaatttt tagatcattt atggaagata
    11821 cgtcagaatc aaaagctttt gctgatcgtg atgacaagtt ttattacaat cgcttaaaat
    11881 attacaaaga gcgtgtgtta gtgcctttag cgtatatcaa ttttgatgaa tatattaaag
    11941 aactaaatga agagcgtgat attttaaata aagatttaaa taaagcatta aaggatattg
    12001 aaaaacgtcc tgaaaataaa aaagcacata acaagcgaga taacttacaa caacaacttg
    12061 atgcaaatga gcaaaagatt gaagaaggta aacgtcttca agaagaacat ggtaatgaat
    12121 tacctatctc tgctggtttc ttctttatca atccatttga agttgtttat tatgctggtg
    12181 gtacatcaaa tgcattccgt cattttgccg gaagttatgc agtgcaatgg gaaatgatta
    12241 attatgcatt aaatcatggc attgaccgtt ataatttcta tggtgttagt ggtaaattta
    12301 cagaagatgc tgaagatgct ggtgtagtta aattcaaaaa aggttacaat gctgaaatta
    12361 ttgaatatgt tggtgacttt attaaaccaa ttaataaacc tgtttacgca gcatataccg
    12421 cacttaaaaa agttaaagac agaatttttt aggaagggaa ttatcaaaac atgaaattta
    12481 cagagttaac tgttaccgaa tttgacaact ttgtacaaaa tccatcattg gaaagtcatt
    12541 atttccaagt aaaagaaaat atagttaccc gtgagaatga tggctttgaa gtagttttat
    12601 taggtattaa agacgacaat aacaaagtaa ttgcagcaag ccttttctct aaaattccta
    12661 ctatgggaag ttatgtttac tattcgaatc gtggtccagt aatggatttt tcagatttag
    12721 gattagttga ttattattta aaagagttag ataaatattt acagcaacat caatgtttat
    12781 atgttaaatt agatccgtat tggttatatc atctatatga taaagatatc gtgccatttg
    12841 aaggtcgcga gaaaaatgat gccttagtaa acttgtttaa atcgcatggt tacgagcatc
    12901 atggctttac aactgagtat gatacatcga gccaagtacg atggatgggc gtattaaacc
    12961 ttgaaggtaa aacacctgaa acattgaaaa agacatttga tagtcaacgt aaacgtaata
    13021 ttaataaagc gataaactat ggtgttaaag taagattcct tgaacgtgat gagttcaatc
    13081 tattcctaga tttatatcgt gaaactgaag agcgtgctgg atttgtatca aaaacagatg
    13141 attattttta taactttatt gacacatatg gagataaagt attagtacca ttagcatata
    13201 ttgaccttga tgaatatgtg ttaaagttgc aacaggaatt gaatgacaaa gaaaatcgtc
    13261 gtgatcaaat gatggcgaaa gaaaacaaat cagataaaca aatgaagaaa attgcagaat
    13321 tagataagca aattgatcat gatcagcatg aattattgaa tgcaagtgaa ttgagcaaaa
    13381 cggacggccc aattctaaac cttgcttctg gcgtttattt tgcaaatgca tatgaagtga
    13441 attatttctc tggtggttca tcagaaaaat acaatcaatt tatgggacct tacatgatgc
    13501 attggtttat gattaactat tgcttcgata atggttatga tcgttataat ttctatggtt
    13561 tatcaggtga ttttacggaa aacagtgaag attatggcgt ataccgcttt aaacgtggat
    13621 ttaatgtaca aatcgaagaa ttaatagggg atttctataa accaattcat aaagtgaaat
    13681 attggttatt cacaacattg gataaattac gtaaaaaatt aaagaa
//
LOCUS       DATTQF010000064        13723 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.064, whole genome shotgun sequence.
ACCESSION   DATTQF010000064 DATTQF010000000
VERSION     DATTQF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.064"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..563
                     /locus_tag="VB241_001538"
     CDS             <1..563
                     /locus_tag="VB241_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1244832.1"
                     /translation="LIAVLKMLIIFLVINYGTDISYDHEYVGLFIILLGVVLLKVLNI
                     NLLSFKKLKFPHMLYIIFGFLLMYGLDYLYDTFAPPTLNEILIDEESEDAPYHIALLS
                     TAIIPPITEEIICRGLIIRILFRNHLFLGFIVSTVFFTLIHESNTLIGYLPYFYSGLI
                     FGYTYLKTKRLEVPILIHFINNLLAM"
     gene            complement(1021..1734)
                     /locus_tag="VB241_001539"
     CDS             complement(1021..1734)
                     /locus_tag="VB241_001539"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEP1244833.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1993..2295
                     /locus_tag="VB241_001540"
     CDS             1993..2295
                     /locus_tag="VB241_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244834.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVHTFGFI"
     gene            2550..2915
                     /gene="crcB"
                     /locus_tag="VB241_001541"
     CDS             2550..2915
                     /gene="crcB"
                     /locus_tag="VB241_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEP1244835.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            2912..3265
                     /locus_tag="VB241_001542"
     CDS             2912..3265
                     /locus_tag="VB241_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEP1244836.1"
                     /translation="MISIILVMIGGGLGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(3515..4348)
                     /locus_tag="VB241_001543"
     CDS             complement(3515..4348)
                     /locus_tag="VB241_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1244837.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFRIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(4560..5468)
                     /locus_tag="VB241_001544"
     CDS             complement(4560..5468)
                     /locus_tag="VB241_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEP1244838.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKHLGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(5593..6786)
                     /locus_tag="VB241_001545"
     CDS             complement(5593..6786)
                     /locus_tag="VB241_001545"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP1244839.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(6892..7003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7158..8750
                     /gene="pckA"
                     /locus_tag="VB241_001546"
     CDS             7158..8750
                     /gene="pckA"
                     /locus_tag="VB241_001546"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEP1244840.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKTQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(9043..9789)
                     /locus_tag="VB241_001547"
     CDS             complement(9043..9789)
                     /locus_tag="VB241_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEP1244841.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVKRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTELLRLLHDKYP
                     QAFIHMVGFSRGGLQGLLTFQDLPVNSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPAILIVHGGKDQQVGIHHAYYLADQLELKGAMHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHR"
     gene            complement(9794..10267)
                     /gene="ytkD"
                     /locus_tag="VB241_001548"
     CDS             complement(9794..10267)
                     /gene="ytkD"
                     /locus_tag="VB241_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HEP1244842.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAITRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            10333..10590
                     /gene="yidD"
                     /locus_tag="VB241_001549"
     CDS             10333..10590
                     /gene="yidD"
                     /locus_tag="VB241_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP1244843.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(10587..11588)
                     /gene="menC"
                     /locus_tag="VB241_001550"
     CDS             complement(10587..11588)
                     /gene="menC"
                     /locus_tag="VB241_001550"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEP1244844.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELNDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLLSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLNRQDFTQLQLLAREQVLYIEEPFTDISMLDDVVDGTIPPIAL
                     DEKATSLSAIINLIKLYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVVGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(11593..13071)
                     /gene="menE"
                     /locus_tag="VB241_001551"
     CDS             complement(11593..13071)
                     /gene="menE"
                     /locus_tag="VB241_001551"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEP1244845.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITTNGLLDNTMGIQYDTSNETVVPKDSPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYDLQK
                     ILLGGAKLSASMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKKGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGIIDAVCVGHPDDTWGQVPKLYFVSESNISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(13230..13685)
                     /locus_tag="VB241_001552"
     CDS             complement(13230..13685)
                     /locus_tag="VB241_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HEP1244846.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNEIIGRIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDELFHLSALQDMNHRPIDIADDVATMDKSPHQNDRITLKLNKNN"
BASE COUNT         4296 a         2584 c         2027 g         4816 t
ORIGIN      
        1 atttaattgc agttttaaaa atgttgatca tttttttagt aattaattat ggaacagaca
       61 tcagttatga ccatgagtat gttgggctat ttattatatt actaggtgtt gtacttctta
      121 aagttttaaa catcaattta ttatctttca aaaaattaaa attccctcat atgctttata
      181 ttatatttgg atttttgttg atgtatggat tagattactt atatgatacg ttcgcaccgc
      241 ctacattaaa tgaaatattg atagatgaag agtctgaaga tgcaccttac cacatagctc
      301 tgcttagtac tgctatcatt ccaccaatta cagaagaaat catctgtcgt ggattgatta
      361 tacgaatttt attcagaaat catcttttct taggttttat agtttcaact gtattcttca
      421 cgctaataca tgaatcaaac acgctgattg gttatcttcc gtatttttat tcaggtttaa
      481 tttttggcta tacttactta aaaacaaaac gattagaggt accgatttta atacatttta
      541 tcaataacct cttagccatg taacaataaa atcattaatt attttgatat ttacatgatg
      601 acaccaaagc ttttacaaag tattaatagg tgactttcca aatgttattg catatttacg
      661 ttctgtaatt atatcaaaac aacaaaacat attaagttta gctatgaaaa aaatctatga
      721 cgatagattt ttttcatagc tattttttat tgttatcgag cctatcccag actctctata
      781 ctgttatatt aacagtcatt gactgaatga taataagctt ttcaattata gaggccccaa
      841 catagagaat ttcaaaaaag aaattctaca ggcaatgcgc gttggggtgt ggggccccaa
      901 cacagagaat ttcgacaaga aattctacag gcaatgaaat acatattaat ttaaaattga
      961 ctccaatatg aaaaaccccc ataagcacaa tgcctatgag ggtttaaata tatttatata
     1021 ttataaaata gaaagacctg aagattgaat atctttcgca aagcctttaa ctgtatctac
     1081 tgataattcg ttaatatcgc gacctaagtt tgtattcact tttttcacaa catctgcagg
     1141 gcatgtaata atatctgcac caatttcatc agcttgaatc acattgaata attcgcggca
     1201 acttgcccat aataatttaa cgccgtcttt actatgcgta acttttacag cctctttcat
     1261 taatggtaat ggatctacgc ctgtatctgc aatacgtcct gcaaatactg aaacatatgt
     1321 tggcacacct tcagttactg cttcagttat ttctttaact tgttcaattg tataaacagc
     1381 cgtaacgttt aatctcacat tgtcagctga aagtttttta attaaaggaa tcgttgattc
     1441 accttttgta tttacaatag gaattttaac aaatacattt tcgccatatt gttttaaaat
     1501 tgctgcttct ttttccatag tttctaaatc gtctgcaaat acttcaaatg aaattgaagc
     1561 atctggaatt tctttcacag cttcttcagc aaaagctttg taatctgtta cgcccgcttt
     1621 cgccattaaa ctaggatttg ttgtaaagcc atccacttgt ttgtttttat aagctgcttt
     1681 catttcttca atatctgcac catccgcaaa tacttctaca tttagtttag ccatataata
     1741 tagcctcctt gattcttatt aaaattttaa caacatctgc atgtcttttt cttacaacca
     1801 tttgtaaaaa atgattttta tttctttgtt atataaaaca tttatatcac gcttttataa
     1861 tatatagcta attttttgcc tatatttctt aaaaatgact ttagcaacat tgtgtcaaca
     1921 aggtaattaa actattgttt tgtgattgtt tatgatacga tatgtaaatg ataaccatcc
     1981 aggaggtcta gcatgtctcg ttcaaaaaaa tacttttact tatctagctt aatgattatt
     2041 ttaagctttt tctttaatac aaataacgtt ttcctaagtg ggctttttaa ttcttttatt
     2101 aaattaatac ttttctgcag tgttattaac tcaattgtac taatattgtc tataattttt
     2161 gcagatcgtt caattaaatc actaaagcct gatgcagatt ggattagaat tgcgagtaaa
     2221 agtttgcctt ggattattct aatcgttatt ttagtacata tcttttcgat tgttcacaca
     2281 ttcggtttta tttaaaaaag ataaaatgtc aatgtagcct taatacaaaa caatacaatg
     2341 tatcatgcta aaataagtaa atccatttga tgacgttgtt gcgtataaaa atattagatt
     2401 ttcgaaatca taactatgca tctaatcgct atagttatac aacaagatat aacatataat
     2461 gaggtttgat aatgcatcga caatttttgt cgtcgcgttg ccaaaacctc ttttttaaat
     2521 tcaaactact tcttttcgag gtgaaccaaa tgcaatatgt atatattttt atcggtggtg
     2581 ctttaggcgc tttattacgt tacctcattt cttttctgaa tactgacgga ggttttccaa
     2641 tcggaacact gatagccaat ttgactggtg cttttgtaat gggattgcta acagccttaa
     2701 caattgcatt tttttcaaac catccgaccc ttaaaaaagc tattacgact ggttttcttg
     2761 gtgctttaac gactttttca acatttcaat tagaattaat acatatgttt gatcatcaac
     2821 aatttataac tttactacta tatgctgtaa caagttatgt ctttggtatt ttgttatgtt
     2881 acgtcggtat aaaactaggt ggtggtttat catgatatca atcattttag tcatgattgg
     2941 cggcggtttg ggtgcaattg ctagaagtgc cattactgat tatttcaatc ataaattcac
     3001 ttcaaagtta cctatcgcaa cattgatagt aaatcttgtt ggtagttttt taattggatt
     3061 aactataggc ttatcaattt caatctcatg gttccctgcg ttctttgtta ccggtttttt
     3121 aggtggctta acaactttct caacattagc caaggaactt acactaatga tgacgccaaa
     3181 atttaatatt aacctttttc tcaattattc acttttacaa ttcatcattg gatttatagc
     3241 ttgttatatt ggctatcata tttaaaaata aaatgcttca ttcagcaaat aggtaaatta
     3301 cgacaccttc ctgaacgaag cattttttaa ttttcatgca aattttttag caccagataa
     3361 tgcctatcaa aataaaacaa tctttgttgc cgtttaaata atgtgaatgt caataaattc
     3421 tccaaactag tcgaaaataa aaacgttaag caatatgtct attacctaac gttgacaatt
     3481 tatctgacct tttcatcagt tgagcgattt taatctatcc ttcaaaagtt tttggatcag
     3541 gtccaattct cttatcttga tttaaaccat caattcgcgt catttgttca tctgataatt
     3601 cgaaatcaaa tattcggaag ttttcagaga ttctgtttgg tgtcaccgat ttagggattg
     3661 taaccactcc atgctgcaca ttccatctta aaacaacttg ggcaggtgac tttcctaatt
     3721 cttgagcaat gtctttaatt gtctcatcat ttaaaatttg tgcattcatc aatggtgacc
     3781 aagattccat cacgatatgt tgtgctgcca aatataattt caatttatgt tgcgttaaat
     3841 atggatgata ttcaacctga ttaattacag gtttaattga cacttgtgcc aacaaagctt
     3901 ccaaatgttc aggttcaaaa ttgctgacac ctatattttt aactttatta tttttatata
     3961 aatcttccat acctttccat gtatcaacca ttacggcttc gttcgtacct ggccaatgta
     4021 ctagatacaa atctaagtat tttaaaccta atctagataa actagcttcg taggcagctg
     4081 ctacattttc acgaccaaaa tcctcaaaat ataattttga agtaataaat aagtcttctc
     4141 tagcaatacc agttgattcc aatccggcac gaatgccagc acctacttgt tcttcattcc
     4201 cataaacttt tgcggtatca atactacgat atccttgttc aatggcatac ttaacacttt
     4261 ccatgcaatt ttcatcattt tccacacgaa atgtccctaa accaatttgt ggcatcgtgt
     4321 ttccattata aaatgtttta acctccataa atatcgcctc acctttttga tgtattatac
     4381 cctgttatca taacaaatct gagttgaata catgagaaaa aacacttaga gcaatcaacc
     4441 actaaaatgc tagtaatatc tctcaaatat taatcaaatt gtaaaagtaa ttctgtttaa
     4501 tttatgacaa actaaaaaag ccgaagtaac aacatatagt catcacttca gcctaacatt
     4561 taattgaatg attcaatttt atccatcatt tgttgtaagt cttccacgtt gtattgaata
     4621 cgaccatgga atacaaattt gttaaagaac tcgtctaatt gttcagcacc gacaagcact
     4681 ttgacagcac tattttgatt ataatttgaa attgttacat cgccttcatt tttaggatta
     4741 aagtataaaa ttgaagtcgg tgtatattta gcacctaaat gtttttgtaa gtcttcagcc
     4801 aattgtttaa ttgcctcaat ttgatctgaa taatttacaa atgataatga acgtttgtca
     4861 tcattttgat ccataacaat agtttgcggt ctagatttat ctaaatccaa tgtatcaaat
     4921 acttgttcca taggtggtaa atctttaaat tgaccgccac ttataccatt ataaacatga
     4981 ccttttaaca attgagaatc aataatataa agaccagttc ttgttaatac taaatgacta
     5041 attcgttcaa tattattaaa gccatccttt ggtaaaaaga tatttgccat aatgtgcata
     5101 tcttctggtc gaattcgttt ttctttaact aatctttcac gaataccaat taatctcatg
     5161 tccgttacat attcactatg atttttcgag aacaatttta atgcgtcaat ctcacgatct
     5221 tttgtactaa ccatgtgatt ataatcttct tgttgttttg taattgtctt tttattttga
     5281 atacgctctt tctctaaagc ttcttcatga gactttttaa tgttttgttc ttgttgttca
     5341 tacttttctt ctgtttgtcg cttaactttt ttcttactac ctaaggcaac taagaaaagg
     5401 acaaaaaaga ttaatgcaat gactactgca ataatgagtc caatgactac cggtgaagat
     5461 aaatccatca caacaacgct cctttttaat atatgaataa ctttaattat aatagaaaag
     5521 ctaaagattt tcgatacata ttatcattta tataccgaaa atcttttatt tagctatatt
     5581 caattcatct tattatttta ctgcgtcttt taattcttcc actttgtcta atttttccca
     5641 tgggaataag acatctgtac gtccaaaatg accataagca gcagtttgtt tgtaaatcgg
     5701 ttgtttcaaa tcaagcattt taataatacc tgcaggtctt aggtcaaagt gttttctaac
     5761 tgcttcgaca agttgccctt cagaaacttt acctgttcca aatgtatcaa ttgcaattga
     5821 cactggttct gcaacaccaa tcgcatatgc caattgtact tcacattgat ctgctaaacc
     5881 tgctgctaca atatttttag ccacataacg tgcagcgtat gcagctgaac ggtctacttt
     5941 tgtaggatct ttaccgctaa agcatccgcc accatgacgt gcatagccac cgtacgtatc
     6001 aacaatgatt ttacgtcctg ttaatcctgc atcaccttga ggtccaccga ttacaaagcg
     6061 tcctgtagga ttgatgtaga atttagtttg ttcattaatc aagttttctg gaacagttgg
     6121 ataaatgaca tgcgctttga tgtcttcttg aatttgttca agtgtcacat catcagcatg
     6181 ttgtgttgat acgacaatcg tatcaatacg tacagggtta tcattttcat catattcaac
     6241 agtgacctga actttaccgt ctggtcgtaa ataatttaac gtaccatctt tacgcacatc
     6301 tgataaacgt tttgccaatt gatgtgataa ataaattgct agaggcatat acgtctctgt
     6361 ttcattcgtt gcgtaaccaa acattaaacc ttggtcacct gcacctgttg cttcaatttc
     6421 ttcttcgcta tctttatcac gatactctaa tgctttatcc acgccttgtg caatgtcagg
     6481 tgattgttca tcaatcgcag ttaaaattgc cattgtttca taatcataac catattttgc
     6541 tcttgtgtat ccaatttctt tgattgtttc tctaacaact ttcggaatat caacatatgt
     6601 tgttgtagaa atttcgccgg cgatcaatgc catacctgtt gtaacagttg tttcacaagc
     6661 tacacgtgca tttggatcgt cttttaaaat agcatctaat attgcatctg acacttggtc
     6721 agcgatttta tctgggtgtc cttctgtaac agactctgaa gtaaataatc gtttgttatt
     6781 taacatagtt tgctccttta aatttatatt acgaaaattc tctctctgtg agctaaataa
     6841 aaaagacctt ctaactatta atatagagag aaggcctaat acgtccattc gctcttatcg
     6901 ttcagaccta tttgtctgca aacggtttgg cacctttctt ttataaaaaa agaggttgct
     6961 gggtttcatt gggtccatgt ccctccacca ctcaggataa gagaatccgt taaaaataat
     7021 actacctaat taatgaatta atgtcaattt ttcacaaata aatttacagt aaaatattgt
     7081 agattaatta tgttaatgtg ttatactaat taaatgtaaa ggcttacatt taaattatcg
     7141 ctttggaggg atttaggatg tcagtagaca catacactga aacaactaaa attgacaaat
     7201 tactgaaaaa accaacgtca cattttcaac tttcgacgac acaactttat aataaaatct
     7261 tagacaataa cgaaggggta ttaacagaac ttggtgctgt taatgcaagt actggaaaat
     7321 atactggtcg ttcgcctaaa gacaaatttt ttgtctcaga accttcatat agagataaca
     7381 ttgactgggg agaaattaat caacctatcg atgaagaaac tttcttgaag ttataccata
     7441 aagtactaga ctatttagat aaaaaagatg aattatacgt atttaaaggc tacgctggta
     7501 gcgataaaga tacaatgtta aaacttacag tcatcaatga attagcatgg cataatttat
     7561 ttgctaaaaa tatgtttatt agacctgaat caaaagaaga agctacaaag attaaaccta
     7621 acttcactat cgtttctgcc ccacatttta aagcagatcc agaagttgat ggtactaaat
     7681 ctgaaacctt tgtcattatt tcatttaaac acaaagtcat tttaatcggc ggtactgaat
     7741 acgctggtga aatgaaaaaa ggtatcttct ctgtaatgaa ttatctctta ccgatgcaag
     7801 atattatgag catgcattgc tcagcaaacg ttggtgaaaa aggcgatgtt gcattattct
     7861 ttggtctatc tggcacaggt aaaacaacct tatcggctga cccacaccgt aaactaatcg
     7921 gtgatgatga acacggctgg aataaaaacg gggtctttaa tatcgaaggt ggctgctatg
     7981 caaaagcaat taatctttcc aaagaaaaag aaccacagat ttttgacgca atcaaatatg
     8041 gtgcaatttt agagaacact gtagttgcag aagatggttc agtggacttt gaagacaatc
     8101 gttatacaga aaacacgcgt gccgcttatc caattaatca cattgacaat attgtagtac
     8161 catctaaagc agcacatcca aatacaatta ttttcttaac tgcggatgca tttggtgtta
     8221 ttccaccgat ttcaaagtta aataaagacc aagcaatgta tcatttcttg agtggtttca
     8281 cttctaaatt agctggcaca gagcgtggtg tgacagaacc tgaaccatca ttctcaacat
     8341 gtttcggcgc accgttcttc ccgttacacc ctactgttta cgctgattta ttaggtgaac
     8401 ttatcgattt acatgatgtt gatgtttatc ttgttaatac tggatggact ggcggaaaat
     8461 atggtgtagg acgtagaatc agcttacatt acacacgtca aatggtaaac caagcgattt
     8521 ctggcaaatt gaaaaatgca gaatatacaa aggatagtac gtttggttta agcattcctg
     8581 tagaaattga agatgtaccg aaaactattt taaatccaat taatgcttgg agcgacaaag
     8641 agaaatataa aacacaagca gaagatttaa ttcaacgttt tgaaaagaac ttcgaaaaat
     8701 ttggtgaaaa agttgaacat attgccgaaa aaggtagctt caacaaataa atttaaatac
     8761 taattaaaga gccaaccgct gttaaatttt aacaatggtt ggctcctctt attttatatg
     8821 gtaaaaattt ataggcaatt ttattacaac aatcacattt aaggtgacct tcatgccaaa
     8881 ctcacatcac tcattcaatg gaagcagcac gtcttcacat aaagtaccga ttccttacgc
     8941 tatgcatgtt gccccacttt gcataaagta ccgattcctc actcaacgta tatcacacat
     9001 ctccaaagat tgatagttcc catgcgcaca ccacgtttca tactacctat gcaactcaac
     9061 ttggttcata aattctttaa tataagtcaa tgtttcaacc atcgctggtg gtcttggcac
     9121 atgtccttct gccatttgat aaaatgtttc atgcatggca ccttttaact ctagttgatc
     9181 cgctaaataa tacgcatgat gaataccaac ttgctggtct ttccctccat gtacaattaa
     9241 tattgctgga ctgttttcat taatgtttgg aatcgcttgg cgtgcctcat atgctgctcg
     9301 atcttttttc ggatgaccaa tcattctacg taacatccct cttaaatcaa cgcgctcttc
     9361 atacatcaaa tcaatatctg agactccacc ccagatgata taactattta caggtaagtc
     9421 ttgaaacgtc aacaaccctt gtagtccacc tcttgaaaaa ccaaccatat gaataaaagc
     9481 ttgcggatat ttatcatgta gcaacctcaa taattcagtc acatcattta aatcgccacg
     9541 ataaaattca tctttacctt cactcccatt gttacctcgg tagtatggcc caatcactaa
     9601 agtttgacta tctgaaaatt gcattaatct acctgcgcgc acacgtccta cttggccttt
     9661 gccacctcgc aaatatacta caatgcgttt tacttcatga tgtggtgtca tcattaaagc
     9721 ttttacttgt aaatcatctg acaaatatgt aatttcttcg aattgatgcg taaaagattc
     9781 aattggcatt cgtttacgtt tgataaaatc caagtgattg caccctctct acgcatttta
     9841 aaatggtact atcttgcagt aagaaactcc gttgtgcgag ttcaatatca ttgatacagt
     9901 taaacaacac tggccctgct gtttctaaat aatcgttctt gcttaccaat gattcaactt
     9961 cgataaaata tacatctttt acaaaatcag tttgatcatg tgtttcaatg gtatattgcg
    10021 ctatgtaatg aatattttta actttggcgc ctgtttcttc atataattca cgggtaattg
    10081 cttcagcact actttccccg cgttcccttt taccaccagg aaattcaata ccccgtaaat
    10141 tatgtttggt aaaaagcaat tgatttttaa acgttggaat agctagcaca tgattgccat
    10201 ctgctatctc attatccttt ttaaatgtca aattaacttg acgattatct ttatccctaa
    10261 acttcacgcg catcacatcc ctacattgta tgttaatata atagttaatt actatcgttg
    10321 gaggcattaa ttatgaaaaa gatattcttg gcgatgattc atttttatca acgtttcatt
    10381 tcgccactca ctccaccaac ttgtcgtttt tatccaacat gttcagagta cactagagaa
    10441 gcgattcaat accacggtgc tttcaaaggc ctttatttag gcatccgtcg tattttaaaa
    10501 tgtcatccgc ttcataaagg tggctttgac ccagttccgt taaaaaaaga caagtcagca
    10561 agcaagcatt cacataaaca taaccattaa tatggttgta attgagttat atccactaaa
    10621 ggggggcgaa attcgagtcg cccctctttt aatatgcctg aatgcgccac cacatcttgt
    10681 tcaaaataat aacctgctgg tgtaacatct cctggataat cacctttacg agcaagcatc
    10741 gctgtaaaat aacggcttaa accatattca tacatgccgc caacaaccac ttttacacca
    10801 tgacttttca aagtatcaat tgccgtttgc actttatcaa tgccacctag acgaaatggt
    10861 tttaatacaa caactttcac attgtataat tttatcaaat taattatggc cgacagtgac
    10921 gtcgcctttt catcaagagc aattggaggt attgttccat ccactacgtc atcaagcatt
    10981 gagatatctg taaatggctc ttcgatataa agaacctgtt cacgcgctaa taactgtaac
    11041 tgtgtaaaat cttgacgatt caaggattca tttgcatcta taaccaattg aaagtggaag
    11101 tctaattccc gtaacactct aatttgatgc atgatttgag gcgtccattt taatttaatt
    11161 ctggtcggct ttgttgcttt caatgactct agttgtttat ttgataagcc gctcgctgtc
    11221 gctccatatg ctactgaaaa tgaaagcagt acatgaaaca tttgatacaa tgccatgaca
    11281 atagttgccc ttgcagcagg cgtattttcc aatgaatcta ctagttttag tgcagcttca
    11341 tacgtttcaa atgatttatt cctattatct tcgaaccatt gctcaattac atgtttcact
    11401 gacgcaattg tttcatgatc ataccaatct gtttgaaaag cgttacattc cccgaaatat
    11461 gcatttcctt tatcatcatt caattcgata aacaaacaat cacgatgcgt taaagtgact
    11521 ttcggtgtta caatttgtga cttaaatggc tcactatatt tataaaaatg caaagctgtc
    11581 aacttcatca aatcatcctc tatacaactt atttctttgt aatttacctg ttgatgtata
    11641 aggtaaagta tcaacctttt caaagtgttt cggtacttta tatttcgcta aatgttgtga
    11701 taaatatgca atcaattgtg cctttgaaat gttactttca ctgacaaaat ataatttagg
    11761 tacttgtccc caagtatcat caggatgccc tacacatact gcgtcaatga tacctggaaa
    11821 ttgcttcgct accgtttcaa tttgatatgg ataaatattt tcaccgccac taataattaa
    11881 atctttacgt ctgtcataaa tcatgacata accttcatga tctatttcag caatgtcacc
    11941 cgtattaaaa taaccatttt caaacgtacc cgttaaatct gttggataca aatatccatt
    12001 catcacattg gcgcctttaa tcattaattc tccatgacct tttttattag gatttttaat
    12061 ttttacgtct acattggcac ttggcattcc tacagtgtca ggacgtgcat gcaacatttc
    12121 cggtgttgct gttaaaaatt gcgaacatgt ctcagtcatg ccaaatgaat tataaattgg
    12181 caggttatat tgtaatgccg tctctatcat actggcagat agtttagcac cgccgagtaa
    12241 tattttttgt aaatcataag gttcatgtaa accttgttgc ataagccaat ttaaagtttg
    12301 tggcacaagc gaaatgtgtg tgatgcgttc attttttatc atcgttaaaa tttgttcggc
    12361 attgaattta tcaacaatgc gcacagtaaa tccttcaata acagctctta aaagtacact
    12421 gagacccgaa atatgataaa tcggcaagac agatagccaa ttagtatcac gatcaaatcc
    12481 caagctctct ttacatccga ttgcactggc ataatgatta cgaaacgttt gcggcaccgc
    12541 tttttgagga ccagttgtcc ctgatgtaaa cataatcgat gcaatgtcat ctaaattaaa
    12601 tgatgtattt aatatgttgg acggcgaatc tttcggcacc acagtttcat tcgatgtatc
    12661 atattggata cccattgtat tgtccaacaa accgttcgtt gtaatatccg ttccagcgaa
    12721 ttcaatatca tccagcgata caatttgaaa ccctcgcaat tccagtggca aggtgcaaaa
    12781 aatcaattgt acatcgattg acctcatctg attcgtcatc tcattaggtg tcaaccttgt
    12841 attaatcatc gcaatttcaa tatttgccaa ccaacatgca tgtattaaaa tgattgattg
    12901 aatcgaatta tctatgtata gcccgacacg agattgttga taagccttga gccttttagc
    12961 caatagactc gcttcacagt ataaattttg ataagtataa gattcttgac cgtctgttat
    13021 cgcaatatga tgtccatttt gttgtgcttg tttatataac caaaagtcca tgcgctattc
    13081 ctccaaaatc atttacatta taattataac gattttatga cattctagca gtggttatgt
    13141 ttaaaaatat aaaaaagtag acgaattgat gcattgatat gattgttata atgctcaata
    13201 catatcgtta tatcattcgt ctactattat cagttatttt tatttaattt tagtgttatt
    13261 ctgtcattct gatgtggtga tttatccatt gttgccacat catctgcaat gtcaatcggt
    13321 ctatgattca tatcttgtaa tgcacttaaa tgaaatagtt catcatctaa attttcaatg
    13381 agatatacat aatatgttac cttgtccttt ttatatttta acgttttcca aaagtccggc
    13441 ttgcaattca atacattatc cggaatatat tcaataaata agtaacgttt gctgcctact
    13501 ttatctacga aatattttac agtgcctttt tctatacctc ttatatgtgc atagtctgct
    13561 gaaaagtaaa ttctacctat tatttcatta tgttgttgta tttcaaatcg ttggcctact
    13621 attttattgt ttgtgccaca actacttaaa aaaatcagta gatatagcat taaacatatt
    13681 ttcatcccct tgaattttaa aaactttttc aaaagcaaca cct
//
LOCUS       DATTQF010000065        13422 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.065, whole genome shotgun sequence.
ACCESSION   DATTQF010000065 DATTQF010000000
VERSION     DATTQF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.065"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(67..1521)
                     /locus_tag="VB241_001553"
     CDS             complement(67..1521)
                     /locus_tag="VB241_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1244847.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMASIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPKFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVIATLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            1725..3242
                     /locus_tag="VB241_001554"
     CDS             1725..3242
                     /locus_tag="VB241_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEP1244848.1"
                     /translation="MSRQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEVAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            3333..3482
                     /gene="rpmG"
                     /locus_tag="VB241_001555"
     CDS             3333..3482
                     /gene="rpmG"
                     /locus_tag="VB241_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1244849.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            3936..4205
                     /gene="rpsN"
                     /locus_tag="VB241_001556"
     CDS             3936..4205
                     /gene="rpsN"
                     /locus_tag="VB241_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP1244850.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYETLRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            4362..5339
                     /gene="guaC"
                     /locus_tag="VB241_001557"
     CDS             4362..5339
                     /gene="guaC"
                     /locus_tag="VB241_001557"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP1244851.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKSSLTDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            5361..6377
                     /locus_tag="VB241_001558"
     CDS             5361..6377
                     /locus_tag="VB241_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEP1244852.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDGDYN
                     NFIMVSYIKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     IAFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTD"
     gene            complement(6757..7380)
                     /gene="lexA"
                     /locus_tag="VB241_001559"
     CDS             complement(6757..7380)
                     /gene="lexA"
                     /locus_tag="VB241_001559"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEP1244853.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREI"
     gene            7523..7756
                     /locus_tag="VB241_001560"
     CDS             7523..7756
                     /locus_tag="VB241_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244854.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            7892..8131
                     /locus_tag="VB241_001561"
     CDS             7892..8131
                     /locus_tag="VB241_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HEP1244855.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            8252..10240
                     /gene="tkt"
                     /locus_tag="VB241_001562"
     CDS             8252..10240
                     /gene="tkt"
                     /locus_tag="VB241_001562"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP1244856.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFEFGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            10518..10760
                     /locus_tag="VB241_001563"
     CDS             10518..10760
                     /locus_tag="VB241_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP1244857.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            10747..10845
                     /locus_tag="VB241_001564"
     CDS             10747..10845
                     /locus_tag="VB241_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244858.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKDKYVY"
     gene            10939..11406
                     /locus_tag="VB241_001565"
     CDS             10939..11406
                     /locus_tag="VB241_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HEP1244859.1"
                     /translation="MLYLIFSIIVALFMGTLVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLVIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRVAMLYKYKKLKKTLIN"
     gene            11530..12651
                     /locus_tag="VB241_001566"
     CDS             11530..12651
                     /locus_tag="VB241_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEP1244860.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFENNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEGAINILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFSYNEENNAIEISE
                     KDDMSIIEMFYNHITDKELSDIQSNKIKNILENELRKED"
     gene            12655..>13422
                     /locus_tag="VB241_001567"
     CDS             12655..>13422
                     /locus_tag="VB241_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1244861.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNNRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKENAQIENRYQQIDLLWQEIESFDDD
                     KIKGLLEVATQQIDKVIENIPLLQARSKEILAFVNESK"
BASE COUNT         4930 a         1861 c         2432 g         4199 t
ORIGIN      
        1 tttacaccat taaaaaagcg gcaaatgcta tagataatct aaagcatctg ccgtgtggga
       61 atttatttat ttttgattgt cataatcgtg tggttttaaa ttaattgttt ctagctttac
      121 aaattttgtt ttgtgaatga ttttatgaat aaagtaaatc aacgctagaa tgattaaagg
      181 taaaaagttt ttaaaagcat ttaaccattg atcttttaaa atatattcaa ctgaaccacc
      241 aaatagcaag aataatagcg tagcgatgac aatgattggt cctaatggat aaaaaggtgc
      301 tttatatggt aggaccttat taggatcttg accttgtttt ttaatagctt gtcgcaatcg
      361 tatttgtgac caaatgcttg atccccaaac aactataatc attgaaccaa taatttcaag
      421 taaattaaaa acggcatttg aattaaagtt tgcataaata ataacaataa caacgactgc
      481 atatgtagtt aataatgctc ttaatggtaa ttttgttgtc ttgtttaatt tacttaaaaa
      541 tttaggtgct tttttgtctg aacttaagga atacaacatt ctgcctgttg tataaacacc
      601 tgaatttgca gcggataata gtgaagttaa aataaccgca ttgattactg atgctgcaaa
      661 ggctatgcct actctatcga atacaattgt aaatgggctt tgacttattg aactacttgc
      721 tcttaataat gatggatctg tgtacggaat aattgcacca atgactgcaa ttgataatac
      781 atagaataaa agaatacgcc aaaacacttg tttaattgct ttaggcatag actttttagg
      841 atcatctgat tcgccagcag ttactgctac tacttcagta ccaccaactg agaatccagc
      901 gactaataat acgcctagga aaccagagat accaccaaca aacggtgctt ggccttttgt
      961 atagttttca aatccatatg catgaccacc taagataccg aaaatcatta aaaagccaaa
     1021 aataacgaat acgatgattg ttaacacttt aattaatgat aaccaaaact cagtttctcc
     1081 aaatgatttt acagaaaaaa tgtttaataa tagtaaaatt gtaataaaga ttaagctcca
     1141 agtaatgggg tggaaaaatt taaatgtgtc ccagaaataa agcacatttg acgctactat
     1201 gacatcaaca cttgtaacta atgaccacaa tgcccaatac aaccatccca tggtaaagcc
     1261 tagagatgag tcaataaagc gtgttgaata agagctgaat gaacctgata ctggataaaa
     1321 tgttgccaac tctccaattg atgccattaa gaaatatagc atgacaccaa taacaagata
     1381 agcgagtata gcgcctccag gaccagcttg agaaatgata ttaccagtag ctacaaatag
     1441 accagtccca attgcaccac ctatagcaat catggaaatg tgtcttgagt taagactacg
     1501 gttcatttta ttatcttcca tatttagtct cccatctatt taaatatacc cattattgta
     1561 agctttttaa gtgtactatt caataactat ttagcactgt atagcggaaa aattaaaatt
     1621 ttctgatttt ttaatcatct tgagcatgtt taattgtaat tctgatgggg ttaaattata
     1681 atatgtatta aattataatt attataaatt gtggagggat gactatgtca cgacaagata
     1741 aaaagttaac tggtgttttt gggcatccag tatcagatcg agaaaatagt atgacagcag
     1801 ggcctagggg acctctttta atgcaagata tttacttctt agagcaaatg tctcaatttg
     1861 atagagaagt aatacctgag cgtcgtatgc atgccaaagg ttctggtgca tttgggacat
     1921 ttactgtaac taaagatata acaaaatata cgaatgctaa aatattctct gaaataggta
     1981 agcaaaccga aatgtttgcc cgtttctcta ctgtagcagg agaacgtggt gctgctgatg
     2041 cggagcgtga cattcgagga tttgcgttaa agttctacac tgaagaagga aactgggatt
     2101 tagtagggaa taacacacca gtattcttct ttagagatcc aaagttattt gttagtttaa
     2161 atcgtgcggt gaaacgagat cctagaacaa atatgagaga tgcacaaaat aactgggatt
     2221 tctggacggg gcttccagaa gcattgcacc aagtaacgat cttaatgtca gatagaggga
     2281 ttcctaaaga tttgcgtcac atgcatgggt tcggttcaca cacatactct atgtataatg
     2341 attctggtga acgtgtttgg gttaaattcc attttagaac gcaacaaggt attgaaaact
     2401 taactgatga agtagctgct gaaattatag caacagatcg cgattcatct caacgcgatt
     2461 tattcgaagc cattgaaaaa ggtgattatc caaaatggac aatgtatatt caagtaatga
     2521 ctgaggaaca agctaaaaac cataaagata atccatttga tttaacaaaa gtatggtatc
     2581 acgatgagta tcctctaatt gaagttggag agtttgaatt aaatagaaat ccagataatt
     2641 actttatgga tgttgaacaa gctgcgtttg caccaactaa tattattcca ggattagatt
     2701 tttctccaga caaaatgctg caagggcgtt tattctcata tggcgatgcg caaagatatc
     2761 gattaggagt taatcattgg cagattcctg taaaccaacc taaaggtgtt ggtattgaaa
     2821 atatttgtcc ttttagtaga gatggtcaaa tgcgcgtagt tgacaataac caaggtggag
     2881 gaacacatta ttatccaaat aaccatggta aatttgattc tcaacctgaa tataaaaagc
     2941 caccattccc aactgatgga tacggctatg aatataatca acgtcaagat gatgataatt
     3001 attttgaaca accaggtaaa ttgtttagat tacaatcaga ggacgctaaa gaaagaattt
     3061 ttacaaatac agcaaatgca atggaaggcg taacggatga tgttaaacga cgtcatattc
     3121 gtcattgtta caaagctgac ccagaatatg gtaaaggtgt tgcaaaagca ttaggtattg
     3181 atataaattc tattgatctt gaaactgaaa atgatgaaac atacgaaaac tttgaaaaat
     3241 aaatttgata tgtagtttct atattgcgta gttgagcagt ttatgatatc ataataaatc
     3301 gtaaagattc ctaacaagag agggtgttta acatgcgcgt aaacgtaaca ttagcatgca
     3361 cagaatgtgg cgatcgtaac tatatcacta ctaaaaataa acgtaataat cctgagcgta
     3421 ttgaaatgaa aaaatattgc ccaagattaa acaaatatac gttacatcgt gaaactaagt
     3481 aattcttatc attcaaatac gacgatttga aaataaagcg ggcttaccta ttatatgggg
     3541 gagctcgctt ttttatgaaa tttttgtgaa gagtgattaa tgaattgagt ttcatcgata
     3601 gaacaatata tgtttgtatt agttgttact ttattaaaat ttgagaatat tgatagaagg
     3661 aaatagatta ctaattttat aaagtcactt tgttagcgaa tgcttgaaag agtaattaat
     3721 atagtagaat ttaaaatttc aatgcgggat ttaataagta agaagtaatt gtggatatgt
     3781 tttataaatg ttcgacaata cactttgatg ttaaatttga tggtttaaaa aatgatttaa
     3841 caaagaaatg aaactttact gttgaattat gtgaggattg tgttattata taaatcgtaa
     3901 taattacgat ttgataaaaa gtgaggtaac tatatatggc taagaaatct aaaatagcaa
     3961 aagagagaaa aagagaagag ttagtaaata aatattacga attacgtaaa gagttaaaag
     4021 caaaaggtga ttacgaaacg ttaagaaaat taccaagaga ttcatcacct acacgtttaa
     4081 ctagaagatg taaagtaact ggaagaccta gaggtgtatt acgtaaattt gaaatgtctc
     4141 gtattgcgtt tagagaacat gcgcacaaag gacaaattcc aggtgttaaa aaatcaagtt
     4201 ggtaaaatca aatttcgtac tttagcccat ttacaatatc aataaaacaa tgtacagtat
     4261 atacgaatgc tataaactga atgttttctc atattaataa ggaaacattc ggtttttaat
     4321 ttgcatttaa aaataattag tttttaaagg ggctatttaa agtgaaaata tttgattacg
     4381 aagatattca attaatacct aataaatgca tagttgaaag taggtctgaa tgtgatacaa
     4441 ctatccaatt tggtccgaaa aaattcaagc tacctgtagt tcctgcaaat atgcaaacag
     4501 ttatgaatga gaaattagcg aaatggtttg ctgaaaatga ttacttttat atcatgcatc
     4561 gttttgatga agaagcaaga atacctttta taaaacatat gcaaaattca ggcttatttg
     4621 catctatttc agttggtgta aagaaagcgg aatttgattt tatagaaaaa ttagctcaag
     4681 aaaagttaat ccccgaatat attacaattg atattgcgca tggtcactca gattcagtga
     4741 taaacatgat taaacatatt aaaacccata tacctgatag ttttgttatt gctggtaatg
     4801 ttggtacgcc agaaggtgtt agagaattag aaaatgctgg tgctgatgct accaaagtcg
     4861 gtataggtcc tggtagagtt tgtattacaa aaattaaaac aggttttggt actggtggtt
     4921 ggcagttagc ggcattaaac atatgtagta aagcagctcg taaacctttg attgccgatg
     4981 gtggtataag aacgcatggc gatatcgcta agtcaattag atttggtgca tcaatggtca
     5041 tgattggttc attatttgcg gcacacgaag aatcacctgg tgaaactgta gaacttgatg
     5101 gtaaacagta taaagaatat tttggtagtg catctgaatt tcaaaaaggc gaacataaaa
     5161 atgttgaagg taaaaaaatg tttgtagaac ataaaagttc gttaacggat acgctaaaag
     5221 aaatgcaaca agatttacaa agttcaatct cttatgccgg tggaaaagac ttgaaatcat
     5281 tacgtactgt agattatgtt attgttagaa actctatttt caacggtgat agagattaat
     5341 atttatagta ggtgatgtaa attaaaaaat tcatagtaac tgttgttgca tttttatcaa
     5401 ttattatcat tgcgccaata acagaattta aaccattcat tcatttacaa aatgaagtaa
     5461 gacaatatat tgacattcac atcaataaag aaacaatttc tgcggaaaat aaattggata
     5521 cgccgaagaa acaacaattt gcctttaaca atatacaaat gaacatgtcg aaatcagatg
     5581 ttgagaaaac attaaataaa ccaaaaagag tgacatttaa tgaatatggt acgaagtggt
     5641 atacgtatta tgatggtgat tacaataatt ttatcatggt aagttacatt aaagataaag
     5701 ttaatgcgtt atatacaaat caaaatatca tcacttcaaa atcaaaaatt aaatacaata
     5761 caccgaaatc ggttgtaagg caaagattag gtgaaccaga aacagagatt gttaaaggta
     5821 gagtgcgtta cgaacaaaat aataaagaat atgatgtttt ccataaaaat cacatttata
     5881 cgacggtatt ttatgataag catcgacgta ataatgtaac agctgtttta caagtaagtg
     5941 atgctatgga aaatagatta aaagaacaat atggagcacc ttcgaaatcg cttgcagata
     6001 gttttgaact acaaaatttt gatttagtta atgctgaaag aaaacaacat caattatcta
     6061 cattgaagta ttctaaacag aattctgaaa ctgctcgtaa gcatagtaaa gatatggcca
     6121 aaaatcatta ttttgatcat acaaatttaa aaggtcaatc accatttgat cgattgaaaa
     6181 aagatggtat tgcatttaac tcagccggag agaatttagc atatggtcaa gttagtagta
     6241 tctatgcaca tcaaggatta atgaattcta ttggtcacag aaaaaatatt ttaaatgata
     6301 cgtttaaaat attaggcgtt ggtgttgatt ttaatgatga aaaacaacct ttttggacag
     6361 aaaattatac tgattaatgt ttttggcatg agattaaagg gtaatgtttg tctgtaagga
     6421 agtagataaa catgatgaca acagataaac caaaagatgc ggatatactt gaacgcgtca
     6481 aagatatact taataaaaaa gaaagaaaaa aataataatg gctcgctctt gtaaattatt
     6541 acgggggcga gttttaattt gtgtaacatt ttgcaggaat ctgtaattca aaatttatat
     6601 taaagaatga gattatctaa caattcgcaa tttctgtttg aactgaattt agattagaat
     6661 aaataaaacc tcttgaaaac gtaacttttt caagaggtcc aaaatttata aaaatgaggg
     6721 cttaacaatt acgataatat ataacggttt aaaatattat atttcgcggt acaaaccaat
     6781 tactttcccg attacagcaa cattgtcgag gtaaattggc tccattgtac tattttcagg
     6841 ttgtaatcga taacgatttt tttctttata gaagcgtttg acagttgctt catcttcctc
     6901 agtcatagca acaataatgt ctccattttc tgctatggtt tgactgcgaa caattacttt
     6961 gtctccgtct aatataccag cctcaatcat actgtcgcct acgacgttta atatgaatat
     7021 gtcgctattg tgtgtcgatg ttaagtgttc aggtaatgga aaatattctt caatattttc
     7081 tactgcggta ataggaacac ctgctgtgac tttaccaata actggcacat gaatcgtttc
     7141 ttccatatta atattatcat ttgtttgatc acttacaatt tctatagcac gtggtttcgt
     7201 tggatctctt cttatatagc ctttttcttc aagacgtgaa aggtgaccat gaacagttga
     7261 actggatgct aagccaactg cttcaccaat ttcgcgaaca ctaggcggat aacctttcgt
     7321 ttgaacaact tgtttaatat agttatatat ttcgctttgt cgttttgtta attctctcat
     7381 atataggcac tccctaaatt aatttaaata cagtatatca ttaattcaca accaaaacaa
     7441 acatttgttc gttaaaatat tgacacagaa cataagttct gataaattac taatacaaac
     7501 aaatgttcta ggagtgaaaa tgatgtttta caataaatat aaaaacgtat caacatatat
     7561 catcatattt ttagtttcaa gtgcagcctt tgcaatattc ttgttaagtg cgaacgttag
     7621 tgctcactcg gaacaagtgt acgaaatgac tgaccatcaa attaagaaca atacgataaa
     7681 taaagcatac gaacataaag accctacaaa caatagcgaa caaagagatg ggaaagtgtt
     7741 cgctttaata aattgataca ttgtcacaac gttattttgc ctatttttgc gaaatagcgt
     7801 tttttattac ttttttgctg atcttaaatt tgttatattt tgttaaagta ttataatgat
     7861 tgaataaaca aattgaaggt aggtttttta attgagtaat tctgatttga atatcgaaag
     7921 aattaacgag ttagctaaaa agaaaaaaga agtaggatta actcaagaag aagcaaagga
     7981 gcaaacagcg ttaagaaaag cttatcttga gagttttaga aaagggttta aacaacaaat
     8041 tgaaaatact aaagtaattg atccagaagg taatgatgta acacctgaaa aaattaaaga
     8101 gatacaacaa aaaagagata ataaaaatta aaacacaaat ctgtaaagaa ttttctgaca
     8161 ttataacttg aaataagtat tttacttatc tttttatttt aaaataagtt ataatgtatt
     8221 tgataaaatt gaagaaggga agatacacaa gatgtttaat gaaaaagatc aattagctgt
     8281 tgatacgcta cgtgcactaa gtatcgacac aatcgaaaaa gcgaattctg gtcatccagg
     8341 attacctatg ggagctgccc caatggctta cactttgtgg acacgtcatc tgaattttaa
     8401 tccacaatct aaagattact tcaatagaga ccgtttcgta ttatctgcag ggcatggttc
     8461 agcactattg tatagcttgt tacatgtttc tggtagttta gaattagaag aattaaagca
     8521 atttagacaa tggggttcta aaacaccagg tcatcctgaa tacagacata cagatggtgt
     8581 agaagttact actggaccac ttggacaagg ttttgctatg tcagttggat tggctttagc
     8641 agaagatcac ctagcaggga aatttaataa agaaggatat aatgttgtag atcattacac
     8701 atatgtatta gcttctgacg gtgatttaat ggaaggtatt tcgcatgaag cagcttcatt
     8761 tgctggacat aataaattaa gtaaattagt tgttttatac gattcaaatg atatttcatt
     8821 agatggcgaa ttaaacaaag ctttttctga aaacacaaaa gctcgttttg aagcatatgg
     8881 ttggaattac ttactagtta aagatggtaa tgatttagaa gaaattgata aagcgattac
     8941 tacagctaaa tctcaagaag gaccaacaat tattgaagtt aaaacaacaa tcggatttgg
     9001 ttcaccgaat aaagcaggaa ctaatggtgt tcatggggca cctttaggtg aagatgaaag
     9061 aaaattaaca ttcgaaaatt acggtttaga tcctgaaaaa cgttttaatg tttcagaaga
     9121 agtatacgaa attttccaaa atactatgtt aaaacgtgct aatgaagatg aatctcaatg
     9181 gaattcatta ttagaaaaat atgcagaaac atatcctgaa ttagcagaag aatttaaatt
     9241 agcgattagt ggtaaattgc ctaaaaatta taaggatgaa ttaccacgtt ttgaatttgg
     9301 tcataatggt gcatctcgtg ctgattctgg tactgttatt caagcaatca gtaaaactgt
     9361 cccttcattc tttggtggat cagcagacct tgctggttca aacaaatcca atgtaaatga
     9421 tgcaacagat tatagttctg aaacacctga aggtaaaaat gtgtggtttg gtgtacgtga
     9481 atttgctatg ggtgctgctg taaatggtat ggctgcacat ggaggtttac atccatatgg
     9541 tgcaacattc ttcgtattta gtgattattt aaaaccagcg ttacgtttat catcaattat
     9601 gggattaaat gcaacgttca tcttcacgca tgattcaatt gcagtaggtg aagatggtcc
     9661 tactcatgaa ccaattgaac aattagctgg attaagagcc attccaaata tgaatgttat
     9721 ccgtcctgct gatggtaatg aaacaagagt agcatgggaa gttgccttag aatctgaatc
     9781 tacacctact tcattagtat tgacacgtca aaacttaccg gttttagatg taccagaaga
     9841 tgtagttgaa gaaggcgttc gaaaaggtgc ctatacagtt tatggctctg aagagacacc
     9901 agaatttcta ttattagctt caggttcaga agttagtctt gcagttgaag ctgctaaaga
     9961 tcttgaaaaa caaggtaaat cagtgcgtgt tgtttcaatg cctaactgga atgcatttga
    10021 acaacaatct gaagaatata aagaatcagt tattccatca agcgtaacaa aacgtgttgc
    10081 gattgaaatg gcttcaccgc ttggatggca taaatatgta ggtactgcag gtaaagttat
    10141 tgctattgac ggctttggcg caagtgcacc tggcgattta gtagttgaaa aatatggctt
    10201 tacaaaagaa aatatcttaa accaagttat gagcttataa gaataattta taaagcgagt
    10261 atgtttagaa gtctaggatg cataatctta ggcttctttt aaagtgttga aatttagagt
    10321 atcgcactca aactacatca taagtgataa gttttgaaaa tatactattt cagattaatc
    10381 tttaaaacct ctgttataac agcatgattt ttgatattat ttttagtatc gatattaaaa
    10441 tacttgaata aactagttct tgaaataatg tgatgaattt agtaaaattc agtaggataa
    10501 agaaagcggg tgaaacaatg gcaacttggt tagcaattat ttttatagta gctgcattaa
    10561 ttttaggttt aattggaggt ttccttttag ctagaaaata tatgatggac tacttgaaga
    10621 aaaacccacc aatcaacgaa gaaatgcttc gtatgatgat gatgcaaatg ggtcaaaaac
    10681 cttctcagaa gaaaattaat caaatgatga cgatgatgaa taaaaatatg gatcaaaata
    10741 tgaagagtgc gaaaaagtaa attcgcaatt gatagaggct attttccaga tatggaaatg
    10801 gcctcttttt ataatcaaat taataaggat aaatatgttt attaaaatta aagttaacaa
    10861 aatgacgaat agactgagaa atgctataat tcattttgta tgatttacag agagtttatt
    10921 taacgagaag gtgtctgcgt gctctattta atattttcaa tcattgtagc tttatttatg
    10981 ggaactctag ttatagttat tcgtatgaaa gctcaaaatt atccggtaaa tgagaaaaaa
    11041 atagttttgc caccgttttt tatggcgact ggtgcattga tgtacgtcgt tccatatttt
    11101 aggctaacag gatcggaaat gctagaagcc tttataattg gtttgctttt ttctacagtt
    11161 ctaatttgga cttctcgatt tgaagtcaaa ggtacagaaa tttatatgaa acgatctaaa
    11221 gcatttccag ttattttgat ttcattactt gtcattcgta ctgtgatgaa aatattcatt
    11281 agtaatgaaa tagatcctgg agaattaggc ggcatgttct ttttattagc attctgtatg
    11341 attgttcctt ggagagtagc aatgctatat aaatacaaaa aactaaagaa aacattaatc
    11401 aattaattac ttctaaaacc acttgtgatc gacttctaaa tcagtcaatg agtggtttta
    11461 attttacttg aaaaggtgaa atgattatat taaaataaaa gcgaacaaat gttctatatg
    11521 gaggcagtaa tgaaaattat acatacagca gactggcact tagggaaaat attaaatggc
    11581 aaacagcttt tagaagatca agcgtatatt ttagatatgt tcgtagaaaa aatgaaagaa
    11641 gaagaacctg atatcattgt gatagctgga gatttatatg acacaacata tccaagtaaa
    11701 gatgcaatca tgttattaga acaagcgatt ggaaagctga atttagaact gcgtatacca
    11761 ataattatga ttagcggaaa tcacgatggt aaagagagat taaactatgg ggcgagttgg
    11821 tttgaaaata atcagttatt tataagaaca gattttacat cgattaattc accaatagag
    11881 ataaatgggg ttaattttta tacactccct tatgctactg tgagcgaaat gaaacactat
    11941 tttgaagatg acaccattga aacgcatcaa caaggaatta cgcgctgtat tgaaacaata
    12001 gcaccggaaa ttgatgaagg tgccatcaat attttaatta gtcatctgac tgttcaaggt
    12061 ggaaagacat ctgattctga aagaccatta actattggaa cggttgaatc agttcagaaa
    12121 ggtgtttttg atatatttga ttatgtcatg ctaggtcact tgcatcatcc atttagtata
    12181 gaagacgaca aaattaaata tagtggctcc ttattgcagt attcattttc ggaagcgggt
    12241 caagcaaaag ggtatagacg tgtaacaatt aatgatggca ttattaacga tgtatttatt
    12301 cctcttaagc cgcttagaca attggaaatt atctcgggcg aatataatga tgttattaat
    12361 gaaaaagttc atgtgaaaaa taaagataat tatttacatt ttaaacttaa aaatatgtct
    12421 catattactg atccaatgat gagtttaaaa caaatttatc ctaatacttt agcgctgacg
    12481 aatgaaactt ttagttacaa tgaagaaaat aatgctatag aaataagtga aaaagatgac
    12541 atgtcaatca tcgaaatgtt ttataaccat ataactgata aagaattatc ggatatccaa
    12601 tctaataaga taaaaaatat tttagaaaac gaattgagaa aggaggatta atgaatgaaa
    12661 ccattacatt taaagttgaa taatttcggc ccctttttaa aagaagaaat tgatttttct
    12721 aaaattgata ataatgaatt gtttttaata agtggtaaga ctggatcggg taaaacaatg
    12781 atttttgatg caatgactta tgccttgttt ggtaaagcat caactgaaca aagagaagaa
    12841 aatgatttga gaagtcattt cgctgatggt aaacagccga tgtcagtaac gtttgaattt
    12901 caattaaata atcgaattta taaagtgcat agacaaggcc cttatatcaa agaaggtaat
    12961 acaacaaaaa cgaacgctaa atttgatgta tttgagatgg tggatggcaa gtatgaaatt
    13021 agagaaagta aagtaatttc aggtacccaa ttcattattg aattattagg agtaaatgca
    13081 gatcaattcc gacaattgtt tattttgcct caaggtgaat tcaaacgctt tttaatatca
    13141 aacagtcgtg aaaagcaagg gatattaaga acgctgtttg acagtgaaaa atttgaagct
    13201 atacgagaaa tattaaaaga agaagtaaaa aaagaaaatg ctcaaatcga gaatagatat
    13261 caacaaattg accttttatg gcaagaaatt gaatcatttg atgatgacaa aataaaaggc
    13321 ttattagagg ttgccactca acagatagac aaagtgattg aaaatatacc acttttacaa
    13381 gctaggtcga aagaaatact agcatttgta aatgaaagta aa
//
LOCUS       DATTQF010000066        13030 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.066, whole genome shotgun sequence.
ACCESSION   DATTQF010000066 DATTQF010000000
VERSION     DATTQF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.066"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(165..440)
                     /locus_tag="VB241_001568"
     CDS             complement(165..440)
                     /locus_tag="VB241_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP1244862.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(447..911)
                     /locus_tag="VB241_001569"
     CDS             complement(447..911)
                     /locus_tag="VB241_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP1244863.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIQDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            1058..1258
                     /locus_tag="VB241_001570"
     CDS             1058..1258
                     /locus_tag="VB241_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HEP1244864.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            1261..2517
                     /locus_tag="VB241_001571"
     CDS             1261..2517
                     /locus_tag="VB241_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEP1244865.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYRCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            2607..3137
                     /locus_tag="VB241_001572"
     CDS             2607..3137
                     /locus_tag="VB241_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEP1244866.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3397..4548)
                     /gene="yfkAB"
                     /locus_tag="VB241_001573"
     CDS             complement(3397..4548)
                     /gene="yfkAB"
                     /locus_tag="VB241_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HEP1244867.1"
                     /translation="MNSVKLKQPVSIHNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHNRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNACTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            4772..4933
                     /locus_tag="VB241_001574"
     CDS             4772..4933
                     /locus_tag="VB241_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244868.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            5063..5578
                     /locus_tag="VB241_001575"
     CDS             5063..5578
                     /locus_tag="VB241_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP1244869.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVF
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            5919..6728
                     /gene="sgtB"
                     /locus_tag="VB241_001576"
     CDS             5919..6728
                     /gene="sgtB"
                     /locus_tag="VB241_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HEP1244870.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            6989..7807
                     /gene="recX"
                     /locus_tag="VB241_001577"
     CDS             6989..7807
                     /gene="recX"
                     /locus_tag="VB241_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP1244871.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            7785..8099
                     /locus_tag="VB241_001578"
     CDS             7785..8099
                     /locus_tag="VB241_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HEP1244872.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            8114..9631
                     /locus_tag="VB241_001579"
     CDS             8114..9631
                     /locus_tag="VB241_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1244873.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAQDIDLNNISLHI
                     YQGEALGIIGEPESSKAMVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINNHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDLQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPFMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            9640..10476
                     /locus_tag="VB241_001580"
     CDS             9640..10476
                     /locus_tag="VB241_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244874.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRLIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGICVLCFLVLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSKDSTDEADT
                     SS"
     gene            complement(10737..11714)
                     /locus_tag="VB241_001581"
     CDS             complement(10737..11714)
                     /locus_tag="VB241_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEP1244875.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTHPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVVGLHPLAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQKLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTKDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            11866..12903
                     /gene="mutY"
                     /locus_tag="VB241_001582"
     CDS             11866..12903
                     /gene="mutY"
                     /locus_tag="VB241_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP1244876.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFKSEHARREMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGTINIERLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         4703 a         1828 c         2254 g         4245 t
ORIGIN      
        1 ttaattgatt taaatctttc ataggctatt ttgatttaaa attaaaacaa aggcaatact
       61 gaaacaatgc agtttggcca atcattgtat tatcatttaa taatgataac tacgaaaggc
      121 gtacaccaaa ttgttaagta ttaccttata aaaacaaaat catcttagcg ttgatttttt
      181 ctattaacaa atgtttcttt cgcatccttt aatgaacgtt ctaattcagg attattacga
      241 cgaatttctt caacaacatc tttccaatat aaaacttcgt ctttaatctt gctgacttta
      301 gaaggcttac gagtgcggtt accatttttt gcatctttaa ctgattgaac taaagcttga
      361 cgtgtagatt tatcagctaa actaattgca ccaccaatta cggcaccaat taaaatacca
      421 ggaacaaatt tattttccat aaaaaactac ccctctttca aatttgcatc ttttacgatg
      481 tagtctatta aattatcaca agatgataat accatgtcgt atacaccttc aaaattattc
      541 gtgtagtatg gatctggtac atcactctct tccatattac taaattctaa cagtttgaac
      601 aattgtccct taagattagg attgatagat ttaatattat caacgttact ttgatccata
      661 gccacaatgt aatcaaaatc atctgtcgct tcgaataatt cactaatcat gccatcaaat
      721 ggaatattgt gtttgttaag aattttttgt gtaccttcat gaggcggctc tcctaaattc
      781 cagctaccag tacctcttga atgtactttt atatcttgaa tatttctgtc tttaagtctt
      841 tgtcgcatga ttgcttctgc cattggagaa cgacatatat tgccaagaca gacaaatgct
      901 acatctacca ttttgattcc tccaaactat gtagttatat ccccatttta tagcgacttt
      961 aaacaataag aaagcagatt atataaaatt ctattaaagt ttattaaatt gtgatacttt
     1021 gataacataa ctattattag aggtgaacat tgtggctatg acaaatgaag agaaagtatt
     1081 agctattaga gagaagttaa atattgttaa tcaaggatta ttagatcctg aaaaatataa
     1141 aaatgcaaat gaagaagaat taacagatat atatgatttt gttcaatcaa gagaaagatt
     1201 gtcgccaagt gaagtgacag ctattgctga cgctttagga caattgcgac acgactagga
     1261 gtgggaattt tgactaatta caaagaaaag ttacaacaat acgctgaact attagttaag
     1321 gttggtatga atgttcaacc aaaacagcca gtatttatta gatcatcagt agaaacatta
     1381 gaattaacgc atttaattgt agaagaagct tatcgttgtg gtgcttcaga tgtacgtgtt
     1441 gtatatagcg atccaacctt aaaacgctta aaatttgaaa atgaatctgt ggaacatttt
     1501 gctaatcatg agataaaatc atatgatgtt gaagcgagaa tggattacgt taaacgtggc
     1561 gctgctaatt tagcacttat tagtgaagat ccagatttaa tggatggtat cgatagccaa
     1621 aaattacaag catttcaaca acaaaacgca cgcgcattta aaggatatat ggaaagtgta
     1681 caaaaaaatc aatttccatg ggtcgtagct gcttttccat ctaaagcttg ggcgaagaga
     1741 gtatatcctg aactatctgt agaagaagca tatattaagt ttattgacga ggtattcgat
     1801 attgtgcgaa ttgatggtaa cgatccagtt gaaaattggc gtcaacacat cgctaattta
     1861 agtgtttacg cacaaaaatt acagcaaaaa aattaccatg cgttgcatta tgtatctgaa
     1921 ggcactgatt taactgtagg tttagcgaaa aatcatatat gggaagatgc tacaagttat
     1981 gttaacggta aggaacaagc ttttattgct aatataccaa ctgaagaagt atttacagca
     2041 ccagatcgaa atagagtaga tggatatgtt actaataaat tgcctttaag ttataatggc
     2101 acaattatcg atcaatttaa attgatgttt aaagatggag aaattattga tttttcagct
     2161 gaaaaaggtg aagcggtatt gaaagattta atcaatacag atgaaggttc aagaagatta
     2221 ggtgaagttg cgttagtacc tgatgattca ccgatatcaa atcgtaatac cattttttac
     2281 aatactttat ttgatgaaaa tgcagcatgt catttagcga ttggatctgc ttacgccttt
     2341 aatattcaag gtggaacgga aatgactgtt gaggaaaaga ttgcaagcgg attaaatgat
     2401 tcaaacgtac atgtcgactt catgattggt agtagtgatt tgactattta tggcatattc
     2461 gaagatggtt caaaagaact agtatttgaa aatggaaatt gggcatcaac attttaataa
     2521 atgttatttt gaggtgctga gtaggaaatg aaacatgtat ttaaaggtca aatacgtgat
     2581 taaagtatag attgggagat aaaataatga caaatcagga cagaccaatg aaatctatgt
     2641 cagaatcaaa atgttataaa aatagacaag ttttccctca agatacgaat caccatcata
     2701 caatgtttgg tggtacattg atggctaata ttgatgaaat tgcagcaatc acagctatga
     2761 aacatgctgg tgcgcaagta gttaccgcat ctacagactc agtagatttc ttaaagccga
     2821 ttaaaacagg ggacatatta caatacgtag cgatggtttc atacgctggg acaagttcga
     2881 tggaagtggt cgttcaaatt agaattgacg atgtatttaa taacaagcat gatttagctg
     2941 cattaagtta cttaacattt gttgcgttag atgatgaagg gaagcctaaa catgtacctg
     3001 gagtatatcc agaagatgat gttgaaaaat ggttttatga tacagcacca caacgtgttg
     3061 aacgaagaaa agcacgtcgc attgaaagta aacaaacaat tgaatattta gctcaagtgc
     3121 aacatattag agactaaaaa tataaagaca gattgttaag gatgaattga actgttatgt
     3181 tcgaatattc cacctacaat ctgtcttttt gattatgcaa atttatgttt ttagttaaaa
     3241 tttaaccctt ttattataga ctattagtcc tattaaatga cctttattat ttaagtagag
     3301 tgctgccagc aattaattgt tttttatttt gtaatagcat taaaatatgc ggagactact
     3361 tatcgataaa gtgcttctcc gcataattaa gcgtttttaa aattgtataa tttgtggttg
     3421 tttgtgcata tgacgctcat tatctttaaa atccatattc ggatagtaca tatttttaac
     3481 aagaacattt ggtcctaaac aactaaactc ggaacaatga caatttaatg atttagcaag
     3541 atcagaggat aaccatttat caaatacatc tgttaattta tctttttgta tattcgaaat
     3601 tgtacctgtt tcatctccga aatcagttac gattacatta cctgtaaata cattgacatt
     3661 taaacgacta cggccatccg ggtcatttct agtcgttaca tttttagcag ttcttaaacg
     3721 tgatagtaac ttttgatctt catcatcttt taagcatgga aacacaggca aagtaccaaa
     3781 taacatccaa atatcttcat ctctgaaatc caatatatca tgaattgtct ttttcatttc
     3841 cgctagagtt aacacattta attgacttgc aaagtcagct ggatacatag ggtgaatctc
     3901 gtgtctgcta catttcatat catgaacgac ttcttgatgt atttttcgta aatgtggtag
     3961 cgtactttga ttgagcattg tttccgcaga tacaaacatt ccttgttctg ataatgtaca
     4021 tgcattcgaa atcatttgtt catataattt taacttagct tttaacggtg gttgcttctt
     4081 cattgcgcca aagccaacat ttgcgaattc atcagttgtt ccccagttat gtgagatatg
     4141 cataacatcg atatattcag caatatctaa ataacgatct tgaggcaatg ttaggtttga
     4201 attcatttgt gtatatatac ctcgattatg tgcatacttt aatagaggtt taacaacatt
     4261 tctaatagac tttttagaaa acattggttc gccacctgta attgacatcg ttcgcagatt
     4321 tggaatttca tcaagtctac gataaattaa gtccatatct aaaggctcgg ggtcgacagt
     4381 ttgtaaagta taaccaactg cacagtggct acaacgcata ttacaaagat ttgtagttgt
     4441 aaattcgatg ttacttaaag ttaattggcc atgttcttta acatcgttat atgcttccca
     4501 tggatcattg tgaatgctaa caggttgctt caatttgacg ctattcatcg tcttaaactt
     4561 ccttctttct atttggttca tatcggtatg ataccacttc gtattatcac tcgtaacaaa
     4621 ttatctctta attttagtta attgaaaagg ggatttttaa aagtcaaaat aatttgaatg
     4681 tcataaatat agctaagatt atatcgcttt aacgcaattt ctgttaggat aataatgaaa
     4741 aagaaaacaa aataaaagga gttaactttt tatgaatgaa cagcaaacaa tcgaacagat
     4801 aaaagcgcgt ttaaataagt ttattgagga tatcgatcat gtaaatcctg atgaagtacg
     4861 tgttgaagat atagatgaat ggattggatt gttagatcag cttgaagaaa aggttaaatt
     4921 agtatctaag taaatgccac ataagatgtg ttagagttag atatcgcgac agtcgcttaa
     4981 tgtttttata tcttgtatgt ttaatcactg atttacttgt ctatagtatt tatatcaatg
     5041 atatatagaa aggtgatttt caatgactaa aaaagtagca attattctag caaacgaatt
     5101 tgaagatata gaatattcaa gtcctaaaga ggcattagag aatgctggct ttaatactgt
     5161 agtgattgga gatactgcaa atagtgaagt ttttggtaaa cacggtgaaa aagttactgt
     5221 cgatgtaggc attgcagaag ctaaaccaga agattatgat gcattattaa ttcctggagg
     5281 gttttcacca gatcatttac gtggagatac agaaggtcga tatggcacat ttgctaaata
     5341 ctttactaaa aatgatgtac caacatttgc catttgtcat gggccacaaa tactaataga
     5401 tacagacgat ttaaaaggtc gtacgttaac agcagtatta aatgtacgca aagatttatc
     5461 aaatgcaggc gcacatgtag ttgatgagtc agtagttgta gacaacaata ttgtaacaag
     5521 tcgagtacca gacgatttag atgattttaa tcgagaaatt gttaaacaat tacaatagaa
     5581 gtattgtggt tatcgattgt taaaatttat atattttcgt ttgaaatgaa atatggattt
     5641 tagttttaaa aaatgaataa caaataaaag gtcgtctcta ttggcattta atagggatgg
     5701 tcttttattt ttataaaata taacaatatt tgacatgatt aatatttaaa atcgcaatga
     5761 tttatctata atcttttcaa aaaacaaaaa agtaagtatg atggcgtata cgattgtaag
     5821 cccttggact gattttccca aaaagtgtag cctatttgtg atagtactgg taaactcaag
     5881 gtatatacta agtgagtttt aaaagaagga gcaaacgcat gaaaagaagc gataggtact
     5941 caaactcaaa tgaacatttt gagcatatga aacacgaacc tcactataat acgtattatc
     6001 aaccagttgg caaaccgccg aaaaagaaaa aaagtaaacg aatactattg aaaatattat
     6061 taaccattct aatcattatc gcattgttta ttggtatcat gtatttttta tctacacgcg
     6121 ataatgtgga tgaactaaga aaaattgaaa ataaaagtag ttttgtgtca gctgataaca
     6181 tgccagagta tgttaaaggt gcctttattt caatggaaga tgaacgattc tacaatcatc
     6241 atggatttga tttgaaaggt acaactagag ctttattttc aacgattagc gacagagatg
     6301 tgcaaggtgg tagtaccatt acacaacaag ttgtcaaaaa ttatttttat gataatgatc
     6361 gttcatttac tagaaaagta aaagaattat ttgtagctca tcgagttgaa aaacaatata
     6421 ataagaacga aattttaagc ttttatttaa ataatattta ctttggggat aatcaatata
     6481 cgcttgaggg cgcagcaaac cattactttg gaacaaccgt gaataaaaat agtacaacaa
     6541 tgtctcacat tacagtttta caaagcgcta ttttagctag taaagtcaat gcacctagcg
     6601 tatataatat caataatatg tctgagaatt ttacgcaacg tgtaagcacg aacttagaaa
     6661 aaatgaagca acaaaattat atcaatgaaa cacaatatca acaggctatg tcacaattaa
     6721 atcgttaaat acttgcattc atgtgtaaga tacttggaac aataatgtag aaggtataat
     6781 tgaaatgcca taatgataaa gttgaattgt ttctgcttaa taaattttac agatttgctt
     6841 gtaatcacac tttcatttta aaagatgata gtgtgatttt ttgttagcat cgaaaaatat
     6901 ttaaagttaa acatttgcta taggaagtgg cttattgaat aataaaagag tattgtatta
     6961 aaacttgaga aagtaacggg gtgatccagt gccgaagatt actaaaatag aagttcaaaa
     7021 gaaaaacaaa gaacgtttta atcttttttt agatgaacaa tttgaaatgg gtatagatat
     7081 cgatacatta gtcaaattta atttaaaaaa agggcaacaa cttgaagctg ctgacatggc
     7141 agagattcaa aagtatgatc attatcgcat aggtttaaat aaagcaatcc aatatttatc
     7201 atataaaaag agaactgaaa aagaagttat acaatattta caaaaagaag agatatcaga
     7261 gcaagcgatt tctgaagtga ttgaatattg ttatcgcgaa aagttaatcg accatcaaga
     7321 ttatgcggaa agtttaaaaa atacaatgat tcgcacgaca gataaaggac ctaaaattta
     7381 tcaacaaaaa ctttatcaac ttggtataga accaaatatc attgaaatgt ttacagaact
     7441 ttatagagaa caacaggaac ttgatgatat tatccaaatt gctgaaaaaa tatctaaaac
     7501 aaaaaagggg ccacaaaata aggttaaaga aaaagtaatg caatctttaa ttcaaaaagg
     7561 gtttgaaatg gaaaccattc atgctgtatt gaatgaaatg gattttacac aagatgaagc
     7621 ggttttagac gatttattac aacgagattt agaaaaaatt tataataaaa atcgaaagaa
     7681 gtacacgcaa caaaaattaa tttcaaaaac tatcgaaggc cttatgagaa aaggatataa
     7741 atatgataaa attaaagcta aattagaaga aagtggtatt gccgatggaa cagaagaaat
     7801 tgagtgagat gtctgagcca gaattaagac acgaaataca actttataaa gagaaaatga
     7861 gaaaagctga gatgaatggt attttaaatg aatatgacgt ttatcaaagt aaagtaattg
     7921 ttgccgaaag ttacttagta gacagaaaaa agattgaaat tggtaaaatt tataaactga
     7981 ctgatggcag taatcaatat tttaaagtag aaagattaaa aggtattttt gcgtgggggt
     8041 ttagatttaa tagtgatgaa cccgaagaag gcttacctat agcgttatta caattataga
     8101 aggatgaaaa tagatgggaa gttcaattgt tttaaagtta ctaaaagtaa cacactacta
     8161 taggaataaa cagaataaaa aatggtattt accttttgga tatgatgctc aagatatcga
     8221 tttaaacaat attagtttac atatttatca aggtgaagca ttaggtataa ttggtgaacc
     8281 tgaatcttcc aaagcgatgg taggtcaatt gttggcaggt gcaattaaac ccgataaagg
     8341 taaagtagtt tgtactgaag atttgttcta cggatatatt gaagatcaat cgttaattca
     8401 tcaaactgtt gaagcttata cagcgcagtt agttcaacta tttccatatg aaattaataa
     8461 tcataaagct gaacagatta ttcaatatgc acatttaggc gattataaaa cgaagccggt
     8521 taaccatatt tcgaaagcgg catacgctca attactatta agtattgcac gctcatcaaa
     8581 atcaaatatt attattttaa atcatgttat tgactattta acaccacaat ttatggaacg
     8641 tgcgattgaa ttaacaaatg attatattga aaataattta acgattgtgt caattggtga
     8701 tgatattgat aaaatttcac aagtgagtaa ctatatagct tggttttcac atggtcaatt
     8761 aagaatggaa gggtcactta aacaagttat tccatctttt aaagaacatg aacgtgatcg
     8821 attaagtcta aactcaaaag aagaaattga aaactttgat ttagattgga aaaagaatcg
     8881 tacaagaata ccggagatga cctataattt caaacgcgtt gagcgctata atcatgcaaa
     8941 accgcctaag tttttagtgc gtttttggac tttagcctca ggtactattt taggcttagc
     9001 attgatgatg ttgctcattt tcaataatat aggaattatt tcgataacag attttacgaa
     9061 tcgtgctacg atgcaaaatg aaaataaaga tccatatggc gaaaagttag cttatggaat
     9121 tgcttttaat ggcagtgtgg atctgcaagg ggataaacaa gtcacaattc caaaatatag
     9181 tgtagttaca attactggtg aaaatagtaa aaattatcgt gttaccgctg ataataagac
     9241 ttactatgtt agtaaagata aattagaata ttttaacccg gcaggtttat atcaaacgca
     9301 tagttttaaa aaattagcgc catttatgaa atcaaattat agtaattact atgcatactt
     9361 taacagtcaa ttacataaaa agcatagttc agttattaaa actttagttc ctgatgatga
     9421 taaccgtttc gttgcatccg ttacacaaca accgatacaa ttacttttca atgataataa
     9481 tcagttatac ggttttgttt atccaatcgt agataaaaaa gaattaaaag ataagtttaa
     9541 tattaacaat aacatttgga ttactaaagt tgggaatgga tattgtattg ccaatttaaa
     9601 agaagacaaa tggatttata ttgaattgta ggtgtaaaga tgctagataa tattatttta
     9661 tattttaaaa acttaccgca tactaaaagg tatgtaacag aacgtttaaa acaaagttgg
     9721 aaaagttttc taatcgtatt agctgcatgt ttaatactta ttattgcaag tgaaacgcta
     9781 ttttccttta gtcatttgac cgatgttaaa gaggtgcgat ggttatttag acttattgca
     9841 ttgattgttt ttgcggtatt gatgttcaca atttatatct cttatcatca ttatatgaat
     9901 gactttttag ttactaagtt atttaatatt tccgcagcga cgccagtagt tattatgtct
     9961 attttaagtt ttattatgct agtcattttg acgatgatta gtgcattggt taaaccagta
    10021 aacttcgaaa catcatatat agcgctattc tattttattg tgatggcaac tatttttgta
    10081 ggattaatat cagttacttt tggtcttatt cgactgttaa ctgaaaaaat aaatatcata
    10141 ttttatggga tttgtgtatt gtgtttctta gtgttgccaa ttatttttat accaaatcca
    10201 aatcatgtat ttatcaatca catcctaatg ctgaatccaa tgtattacat tgttaatggt
    10261 atagcacaat ctatcatatt tggtatatca agtatggaaa acattccata tcatttttac
    10321 tttattttat tcttatgttt aatagctgca gtaaatttcg tattagcaag gtatacgaca
    10381 cacgcaattt ataataaaac atctaaagtg acacaaactg ataatcaaca agatgtttca
    10441 aaagatagta cagacgaggc ggatacttct tcatgatgtt tagctgactt gttgtgtaaa
    10501 ttaatctgtg agaatcagaa ataattagaa tataatctag atattgtttt cctatactga
    10561 ttttaatatt tcgagtcatt gaaggtagtt tgttagcgac aatataaata ttaggttttg
    10621 taaaagcatt catagtttta cattaaaaaa ccggacagag taaaaatcgt tttgattttc
    10681 attctgcccg gttgttcatt ttttatagat taactttagt gaataataac ttgaaattat
    10741 ttagcataca gtttacgttg taacttatct tctgtaaata cccagccaat ataagagtgg
    10801 tctatgacat tatcttttgt tacgtgtgca attgccacaa atgaataatg atcatttttt
    10861 aaataacgca aatcaatcaa acgaatttca gtagaaccat ctgctaacgt tgttgtttgc
    10921 caacgataga ttgatgaaaa gtttaaaaaa gtacgtatat ctttattacc tttgactttc
    10981 cataaaatgg agtctgttga taatttttga cgttcaactt tatcactaaa caccacattt
    11041 ctaccatatg cttttccaac atagtcatgt gcatcagttt gtatcgcgac acgccattcc
    11101 ataaatttta ttgttggcgc aacaaatact ttaacagggt gatgctcttg ttgaattgct
    11161 tttaaagctt gttgcttaat tacggctctc attttaaaac gaatcatgta ataaatgatt
    11221 agtaaagcga ttataggaaa gaagactgca agtggatgca agcctacaac ccataataca
    11281 ataccaatac aaagaactgt gaaaataata gggtcaaatg tgttaatcac acttaattga
    11341 atccatttgt ttgtgatagg tctaagagct tgtgtaccat aagaattgaa tatatctaca
    11401 aagacatgta aaaatactgc gagctgagcc cacatccata catgaaatgg gtgggttcca
    11461 ctaaagaacg tgaatattaa aaatgtaatt aaaattggcc atagtattgt gaaagggatg
    11521 gaatgcgtga tacctctatg atgcgaaata tatgttgcat tgtcctttaa tttaagaaca
    11581 gtatccccat caggaattaa tgaaccaacg ataagggttg tagctgttgc accaaacgta
    11641 gaagccattg ctggatcttg agttgcaagt gctgtaaggc ccaccccaat tgcgatatgt
    11701 gttgcagtgt ccatatcagt tcactcattt cataatatta tatatactat tataatccaa
    11761 tttcgcaaat aactaaatta acttacatac taaccatgat tttaatgtac aataaaaaca
    11821 ttatgagtaa agacaattaa ttttggtgag gaaagggttt tataaatgta tcagcagtct
    11881 agttttaaag aaaatttgat acactggttt gatgaaaatc aaagagaaat gccatggcga
    11941 caaacgacca atccgtacta tatttggtta agtgaagtca tgttacaaca gacacaagtt
    12001 aaaacagtta ttgactatta tcatcgtttt gttgaacgat ttccaactgt ggaagttttg
    12061 agtcaagctt cggaagatga agtgttaaag tactgggaag gcttaggata ttatagtaga
    12121 gctcgaaatt ttcatacggc aattaaggaa gttcatgata aatatgaagg cttagttccc
    12181 aaagatcctg atcaatttaa agctttaaaa ggtgttggcc catacacaca agctgcagta
    12241 atgagcattg catataatgt accactagca actgtagacg gaaatgtttt tcgggtatgg
    12301 tctagattga atgatgacta tcgagatatt aaattacaat cgacgaggaa atcgtacgaa
    12361 caagaattat taccgtatgt tactacagaa gctggaacgt ttaatcaagc tatgatggaa
    12421 ttaggggcat taatttgtac accgaaaaat ccattatgct tattttgtcc agttcaagaa
    12481 aactgtgagg catttgataa aggtacattt gaaaagttac cagttaaatc taaaaatgta
    12541 agtaaaaaag taattgagca atccgtcttt ttaattagaa ataatcaagg tcaatatctg
    12601 ttgcaaaagc gtagcgaaaa attattgcat ggtatgtggc aatttcctat gtttaaaagt
    12661 gagcatgcta gacgtgaaat gactgaaaaa atcggtcatg acattcaacc agtagaaaca
    12721 cctatttttg aattgaagca tcaatttact catttaacat ggaagataaa ggtatacgcc
    12781 gtttcaggaa caattaacat tgaaagatta ccagatgaca tgatttggtt tgatttaagt
    12841 gatcgagatc aatatacatt ccctgtacct atgtcaaaga tttatcaatt tataaatggg
    12901 taacattcga tgattgataa gattatcgga aaatatagtg acattaattt taaaaatgta
    12961 taaatgttat cttcaattct cagaggataa caatgtcaaa aagttaaagt gttgatatat
    13021 aaaagtgatg
//
LOCUS       DATTQF010000067        13022 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.067, whole genome shotgun sequence.
ACCESSION   DATTQF010000067 DATTQF010000000
VERSION     DATTQF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.067"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(1..674)
                     /locus_tag="VB241_001583"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="VB241_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(990..2396)
                     /gene="gndA"
                     /locus_tag="VB241_001584"
     CDS             complement(990..2396)
                     /gene="gndA"
                     /locus_tag="VB241_001584"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEP1244877.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(2464..3597)
                     /locus_tag="VB241_001585"
     CDS             complement(2464..3597)
                     /locus_tag="VB241_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEP1244878.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFISLGLDVKE
                     DEAAKHPKLGANNLVCTMKSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNETVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            3860..4039
                     /gene="prli42"
                     /locus_tag="VB241_001586"
     CDS             3860..4039
                     /gene="prli42"
                     /locus_tag="VB241_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HEP1244879.1"
                     /translation="MLVISSQVKNFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(4166..5146)
                     /locus_tag="VB241_001587"
     CDS             complement(4166..5146)
                     /locus_tag="VB241_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP1244880.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(5160..5597)
                     /locus_tag="VB241_001588"
     CDS             complement(5160..5597)
                     /locus_tag="VB241_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEP1244881.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGNITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(5947..7221)
                     /locus_tag="VB241_001589"
     CDS             complement(5947..7221)
                     /locus_tag="VB241_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEP1244882.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASSTVEQTSTSKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDLMSVI
                     ENGGTTAQSDKQVQTQSTSVDRSSNQSSEDDSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNNFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(7234..8217)
                     /locus_tag="VB241_001590"
     CDS             complement(7234..8217)
                     /locus_tag="VB241_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP1244883.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(8217..9209)
                     /locus_tag="VB241_001591"
     CDS             complement(8217..9209)
                     /locus_tag="VB241_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP1244884.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGLHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(9225..10646)
                     /gene="lpdA"
                     /locus_tag="VB241_001592"
     CDS             complement(9225..10646)
                     /gene="lpdA"
                     /locus_tag="VB241_001592"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP1244885.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSVGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(10797..12476)
                     /gene="recN"
                     /locus_tag="VB241_001593"
     CDS             complement(10797..12476)
                     /gene="recN"
                     /locus_tag="VB241_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP1244886.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQGLEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRHR"
     gene            complement(12492..12944)
                     /gene="argR"
                     /locus_tag="VB241_001594"
     CDS             complement(12492..12944)
                     /gene="argR"
                     /locus_tag="VB241_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HEP1244887.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
BASE COUNT         4058 a         2480 c         1773 g         4711 t
ORIGIN      
        1 tctaatagtt gaattagata atagataatc aaatccaact ttaacatcgc tcaaaaaatt
       61 ttttccactc aacttttctt gattgaatat actctttgtt tcaattttaa ttaatatcaa
      121 actgaaaaat gagagtaaaa aagtcataga atctataaaa atcaatattg aatatcccat
      181 atatgtataa agtaaacctc caattatagg gcccaaaaat tccgaaacat tttgagtagc
      241 gtatatataa ctgtttgcgc tcgccaaatc ttcttctttt acaatacttg ggattatcga
      301 aacctcagaa atactgtgaa atactaatcc aatacctgaa atgattgcaa ctacataaat
      361 ttgaaagatg cttaacatat tataaaaact taatactggt atacttaaat atgaaatgag
      421 cctaattata tcagcacatt tcattatggt cttcttattc aatctgtcaa ttaaagcacc
      481 tgctggcagt cctaaaattg cataaggtat aaaacttagt gcagaaacta tgccaacttg
      541 aaatgcagag ccagtaagct ttaaaatcat taacggaaaa caaacgtagt aaaagcactg
      601 cctatgttag aaaaagctat ccctattaaa taaaaagtaa agttcttatt aaacaataca
      661 gaagcatatg acattctatc cctcttcttc atcaaataat aaaacgtaat ctatttatct
      721 aaatattaaa attattaact aatgctatct taatagtatg taagtaaaac gtctacactt
      781 aaacggacaa attctatgag gatagatatc gttaatttat aaaagttaaa aattttaatt
      841 tgacaagaga aagaaaatca ttgaatatat catgcgtagt aaatttcgat aaatacataa
      901 ggaaagagac aatttctatc aaataaataa tagaaattgt ctctttaaca ttacctaaat
      961 tcataaggtc tcaggctcaa tagtcaatat tattcttcaa tccattgtgt atggaatacg
     1021 ccttctttat cttttctttc gtacgtgtga gcaccgaagt agtcacgttg tgcttgaatt
     1081 aagtttgcag gtaaatcagc tgcacggtaa ctatcatagt aattaatact tgatgagaaa
     1141 ccaggtgttg gtacaccatt ttgaacacca gttgcgacaa catcacgtaa cgcatcttga
     1201 tattcagtaa cgatgttttt aaagtaagga tctagcaata agttttgtaa tccaggatta
     1261 ttatcgtaag catctttgat cttttgtaag aattgtgcac ggataatgca accttctctc
     1321 caaatcatag ctaaatcacc aagttttaaa ttccattcat tatcttcact tgctttacgc
     1381 atttgcgcga aaccttgtgc ataggaacaa attttactca tatataatgc tttacgaatt
     1441 ttttctaaaa agtctttctt gtcaccatca aatgatgctt ttggaccatt taattcttta
     1501 gaagcattta cgcgctcttc tttgattgaa gagataaaac gtgcaaatac agattcagtt
     1561 atgattgtta atggaatacc taattctaat gcgttaattg aagtccattt tcctgtacct
     1621 ttttgacctg cagtatcaag aattttttca actaatgctt ctttattttc atctaatttc
     1681 atgaaaatat caccagtgat ttcaattaaa taactttcta attcaccagc attccagtct
     1741 ttgaacgttt gagcaatgtc ttcatgagac atgcctaata attctttcat catagcataa
     1801 ctttctgcaa ttaattgcat gtcagcatat tcgataccat tgtgtaccat tttcacatag
     1861 tgtccagcac catttggtcc aatataagta acacatgaag caccgtcttt tgcctttgca
     1921 gcaattgcat caagaatatc tgcaactttg ttataagctt cttcttgtcc acccggcatt
     1981 aatgacggac cagttaacgc tccaatttca ccaccagaaa cgcccatacc aataaagttg
     2041 attgcacttt gtgctaatgc tttattacgt ctgatagtat cttgatagtt tgtattacca
     2101 ccatcaatta aaatatctcc atcatctaat aaaggtaaca aactatcaat cgttgcatcc
     2161 gtagctttac ctgcttgaac cattaataaa attttacgtg gtttttctaa agaattaaca
     2221 aattcttcca atgaatacgt tggatgaata tttttccctt ttgattcttc aaccattaaa
     2281 tcagtttttt cacttgagcg gttaaataca gatacactat atccgcgtga ttcaatattc
     2341 caagctaggt ttttacccat aacggctaaa ccaataactc caatttgttg tgtcatatta
     2401 cttacctcac ttgttgattt ttcattagta ttgtatcaca aaatagacat acactacact
     2461 aaatcatttc gattgtcgcg caactatttt gattatttct aacacttgac ttgcaagcaa
     2521 gttcaatgat ttaatcggca ttctctcatt tgttgtatgg attttttcat aacccactcc
     2581 taaaatgact gaaggaatac caaatgtatt aataatactg ccgtctgaac caccaccaga
     2641 aataattgta tttgcagata atcctaaatt acgagcactt tcttgtgcaa ttttaacaac
     2701 cgtttcatta tcattaattt taaatcctgg ataactttgc tccactgtaa ctactgcttt
     2761 cccacctaat tctgatgcag tagtttcaaa cacatcagtc atatgtttga cttgtgtttt
     2821 tattctttct ggatcgtgag aacgtgcctc tgcttctaaa atgacttcat ctgcaacaat
     2881 attcgtagct gaaccgccat gaaacttacc aatattggca gtagtgattt catcaacttg
     2941 tcctaatttc attcgactaa ttgctttcgc cgcaatatta atagcactaa caccctcttt
     3001 tggcgtactt gcatgagccg ttttgccaaa aattttagct gaaattaaca tttgcgtcgg
     3061 tgcacctaca accgtagtac cgacatcagc acttgcatca atagcataac caaagtccgc
     3121 gtccaacaac tctgaattta attctttagc accaattaaa cctgattctt ccccaacagt
     3181 aatcacaaat tgaatttgtc catgtgggat ttgttgttcc tttatcactt gcaaaacttc
     3241 aagcatagct gctaatcctg ctttatcatc tgcacctaga atagtcgtac cgtcagagta
     3301 tatgtagccg tcatctttta caattggctt tacattaatt gcgggtacaa cagtatccat
     3361 atggctcgtt agatataatt taggtacttc gccttcttcg atagtacttt tcattgtaca
     3421 cactagatta ttggcaccta atttaggatg tttagccgct tcatcttctt taacatctaa
     3481 ccctaatgat atgaattttt cttttaaaat aggttggatt gttgattcat tccctgtctc
     3541 agaatcgatt tgtacaagtt ctaaaaacgt attaagtaat ctttgctcat taatcataat
     3601 ggaacccctt ttatcaaaat tttcaagtac gccaaacggt aaaaattgat gaattaaaat
     3661 gttacagtaa ttgacgttac acagatttat caggcttgta aattgtgtca tattattttc
     3721 aatttaatat atatatttat tgtaactcaa actaagcttt gtcaaaaata tattgattga
     3781 tttttcaaag atatcgtata atgaggaaaa tgacataagc aaacttactc atgtttttta
     3841 taatattcct ttatgatgat tgctagttat atcgtctcaa gttaaaaatt ttatatctta
     3901 tgtcgtaatt attaatacaa aggttattca tttggaggca cacaaaatgc aaaataaagt
     3961 tttaagaatt atcattatcg ttatgcttgt atcagttgta ttagcattgt tattaacgag
     4021 tatcattcca attttataaa ccatatctca actacctata caaaatcata caattaaaaa
     4081 tccatccgtt ataaacgcat gtattaataa gttatcgtat tgcaacgatt actttcaaac
     4141 atgggtcata cggatggatt attttttaag ctacttcact atgcattttc aatgaaccaa
     4201 attgcgattt gatttgtaaa tattcttcta attcatttaa tatttgaata atacttgctc
     4261 tcgagttaag cgctttgtgt gttgttggca atggcagttc atccaatttc aaacgcgtct
     4321 catacaaatt gtgtaaacgc attgctgtat agtcattact attcacattt agaccaattt
     4381 ctttcagcag tgacgcaaca tcatttaaaa gtggatcttt atgacagata ctttcgatga
     4441 gcggtttcat tctcattaac aattccactt gctcttctcg catatcaaaa taatgatagt
     4501 atgaattttc gtttctaaca aaatgatttt taacatctcg gaacgcgata gacttcgcct
     4561 ttttaatatt taaaagtaac gcttcaaatt caatcgcaat ggtatcttca tatttttcac
     4621 aaatataact atatttacta aaaatatcag caatttgttg ctcaatttta catttgtatt
     4681 cgtctagttg tttgtctaaa cttggcatca ttaaattcat tgtaaatgca atgcttagtc
     4741 caattaacag taataatgtt tcattaacaa ttaaatgtgc atcaattgat tttgcattaa
     4801 aaacatgaag taatataacg caactcgtaa tgacaccttc ttgtactttt aatacgacag
     4861 ttaatggtat aaataacaat acgataatac cgagtacaat tggactctgg cctaataaac
     4921 taaatattgc tgaacctaaa aacaatacta aaaaacatga tactaatctt gaaataatcg
     4981 cttgtagcga atgtactttt gtatgtttaa tacataatac gactaatatg gcacttgaag
     5041 cataattatc taaacctaac agcttactaa taattacacc taaagtcata cccactgctg
     5101 tttttattgt tctaaatcca atcttgtaag gatttaactt taacatgggt tagcgcctct
     5161 tatctttctt cacaatattt attgaataat gtttgtaatt gattaattac gttcatcaca
     5221 tcatgacctt cgatttgatg tctttcaatc atttctgtaa tatttccatc ttttactaat
     5281 gcaaatgacg gacttgaagg cgcataacct tcgaagtatt cacgcgctct ttgtgtcgct
     5341 tctttatctt gtccagcaaa tactgtcact agacgatcag gtaatacgtc ataatgtaaa
     5401 gcatgtgatg ctgctggtct tgcgatacca cctgcacaac cacatacaga attgatcata
     5461 actagtgttg taccatcttg tttaagaact ttgtcaacat cttctgcagt agttaattgc
     5521 tcatatcccg cagattcaat ttcattcctt gcttgttcta caacaccgtt catgtataaa
     5581 tcgaaattca tgtccataag ttcaatcacc tatcccttta tatttaaact atcctcattc
     5641 tactaattaa taacatattg ttcaataaac taatctgaat cacacctata tttagacaca
     5701 attttaacaa tataccaaac attattgtgc ttaaaatcat ggaaactaat ttgttcacct
     5761 gttttcttta atatgtttca agtacgatgt cttattttga ctttactgca aaaatgcatt
     5821 caaccatgtt gattattgtt ctttatcttt ttttgaatat attgcaaata ttttattgcc
     5881 aaaaaaatac atccatcgac aagaacaaga taaaacaagt tgtcgataga tgcatctatg
     5941 ttatcactaa tatatatttg tattttctaa agtatactgt tcgatacgct gtttaatatg
     6001 attcataaat ttacctgttt gtaaaccatc taaaatacga tgatcaattg aaatacataa
     6061 attaaccata cttcgaattg caatcatatc attaattact actggctttt taacgattga
     6121 ttctacttgt aaaatcgctg cttgagggtg atttataata cccattgatg atactgaacc
     6181 aaatgtacca gtattattaa ctgtaaatgt tccaccttgc atatcttcag ttgtcaattg
     6241 cttattacgc gcttttgttg ctaaagtatt aatttctcta gctatacctt tgattgactt
     6301 ttcgtctgca tgcttaatca caggtacgta taatttattt tcatcagcaa cagcaattga
     6361 aatattaatg tctttatgta agacaatttc atttccttgc cagctactat ttaataatgg
     6421 atatgctttt aaagcatctg ctacagcttt tacaaagaaa gcaaagaacg ttaaattata
     6481 tccttcttta tttttaaagt tgtttttata atggtttctc gtattcacaa ggtttgtagc
     6541 atcaacctca atcatcatcc atgcatgtgg aatctccgtt acactattaa ccatgttttg
     6601 tgcaattgct ttacgcacac catttactgg tattgtgcta ttttcactat cgtcttcaga
     6661 tgattggtta cttgatctat ctactgaagt tgattgtgtt tgaacttgtt tgtcagattg
     6721 agctgtggta ccaccatttt caataactga cattaaatcc ttcttagtta cacgaccttc
     6781 aaatccacta cctacaactt gtgataaatc aatgtcatgc tctgaagcga gtttaaatac
     6841 aacaggtgaa aagcgaccat tatttcgtgg ttgattttgt ttagaagtag atgtctgttc
     6901 cactgttgaa ctagcttttt tagtagattt ctgagtatgc tcatccactt ttgcttgtat
     6961 ctcttcagtt gtttcatttg tcttttcatc agcagtttca attttacaga taattgtatc
     7021 aatagctacc gtctgccccg cttcaactaa aatttctgta attgttcctg atatcgtgga
     7081 agggacttca gccgtcactt tatctgtaat aacttcacat aatggttcat attcatcaat
     7141 atgatcacca acagaaacta accattgttc aatggtacct tcatgaacac tctcacctaa
     7201 cttaggcatt gttatttcca tgactttccc tccctagaat tctgctaatt cacgcatttt
     7261 atttaagatt ttttctggat tcatcataat ttcattttct aatacaggtg aaaatggcat
     7321 agatggtaca tctggagcag ctaaacgcat gattggtgca tctaaatcga acaagcaatg
     7381 ctctgcaata atcgctgaca cttctgacat aatgctacct tctaaattat cttcagtaac
     7441 aagtaaaact ttacctgtat gtttagcacg atcaataatt gtttctttat ctaatggata
     7501 aacagttcgt aaatctacaa cttcaacatt gataccgtct gctgctaaaa tatcagcggc
     7561 ttgtaaacaa taattgacca ttaatccata acaaaatact gttaaatctt caccttcacg
     7621 tttaacatct gcttttccta aaggtacagt gtaatattct tctggcactt cttcctttaa
     7681 gaatctataa gcctttttat gttcaaagta taaaacagga tcatttgatt caattgaaga
     7741 taataataga cctttagcat catacggtgt tgaaggtata acaattgtta aacctggtga
     7801 tgaagcaaat atactttcaa tactttgtga atgatatagt cctccgtgaa caccgccacc
     7861 aaatggtgca cgaatcgtta atgggcattg ccaatcatta tttgaacgat aacgcatttt
     7921 cgcagcttca ctaataattt gatttgttgc aggtaaaata aaatctgcaa attgaatttc
     7981 tgcaattggt cttttaccta ccatagctgc accaatggca gttccaacaa tatttgactc
     8041 agctaatggc gtatcgataa ctctatcttc accatattgt tgttgcagtc cttgagtagt
     8101 accaaatacg ccaccttttt taccaacatc ttcaccaaga ataaatacat ctttattttg
     8161 ttgtaaagct aagtcttgtg cctggcgaat cgcctctaaa taagataatt tagccattag
     8221 ttaagactcc cttcttcgta cacaaatgca taggcttctt cgacacttgg atatggcgcg
     8281 tcttcagcag cctttgtcgc tttattgatg atgtctttat gctccgcttc tatttctgcc
     8341 aaccaagcat catcgataat gccagctgaa agcaactctt ttttgaactt ttcattgcag
     8401 tctgcttttt taagcgcttc acgctcttct ttcgtacgat attggtcgtc atcatctgat
     8461 gaatgagctg tcatacgact tgttactgct tcaatcaaag ttgaaccttg accagaaata
     8521 gctcgatctc ttgcttcttt catcgcttta tacattgcta atggatcatt accatctact
     8581 tgttcaccat gtaaaccgta accaagtgct ctatccgata atttttcagc tgcgtattgt
     8641 aatgaatcag gtactgaaat tgcatattta ttatttataa tgacacatac aaaaggaagt
     8701 ttgtgtacac ccgcgaagtt taaaccttca tggaagtcac cctggtttga actaccttca
     8761 ccaacagttg ctgttgcaat tttcttctta ccatccattt ttagagctaa agcagcacca
     8821 acagcatggg gtatttgagt tgctaccggt gaactttgtg acaaaatatt cttagctcta
     8881 ctactaaagt gtgatggcat ttgttttcca ccagagttaa catcgtcttt ctttccaaac
     8941 gctgataaaa acgtatcata cgctgatata cccatataag taacgaaagc tagatctcta
     9001 taataaggcg cagtaatatc accttcttct aatgcgtatg ccatcccaat ctgagttgct
     9061 tcttgtcctt gaccacttac aacaaatgga attttacctg cacggttcaa taaccacagt
     9121 ctttcatcta tttttctacc taaatccatc catttatata ttacttttag gtcttcttcg
     9181 ctaaggccta atgatttata atcaatcatg ttaaatcctc ctatttatac gtgaatagct
     9241 ctactttctg ctttcaatcc taattccatc aacacttcag agatggaagg atgtgcgtgt
     9301 gttgttagtc ctaattctaa tgccgagcca ttcatgaact gtaacagtga tgcctcatta
     9361 atcaattctg ttacatgtgg accaatcata ttaataccca caatttcttc agttgattga
     9421 tcaatcacca tttcgctata cccttcgttt gcgtcatggc tatcaatcac tgctttacca
     9481 attgctttaa atggtacttt aaaactttta actttcattc cctctgcctt tgcttgctca
     9541 atgtttaaac cgatagaagc aatttcaggt tgtgaataaa tacacttagg catcatgtta
     9601 tagtttactg ggattgggtt cccttcaaac atatgatcga cagccacaac accttctttt
     9661 gatccaacat gtgccaattg taattttcca atacaatcac cagctgcata aatatgttta
     9721 tcttcagttt gttgaaattc gttcgttaaa atatgtcctg atgttgaaag ttttatttta
     9781 gtgttgttta aaccaatatc tgatgtgtta ggttttctac caatcgatag caacacttta
     9841 tctactttaa ttatgtctga ggaaatttca aatgtaacac catcttcgtt aacattgata
     9901 tcattttcag aaagttttat tccctcatag aatttaacac cacgtgctga caatgatttt
     9961 tttaatagtt gtgaagcttg tttactttca gttggtaaaa ttctttcacc agcttctata
    10021 actgttacgt caacacctaa atctatcatc aatgatgcaa attccattcc gataacacca
    10081 ccaccaataa taccaacact tgatggtaac gtctttaatg ataatatatc atcgctagat
    10141 aaaattttat catgatcaaa tgataagaat ggcaactctg caggcgaaga accagttgca
    10201 attaatacaa attggttggg taataagtct gattcaccat cttcatattc gacagatatt
    10261 gtgccacttt gaggtgaaaa tatagatgta cctagaatac gtcccgtgcc attataaatg
    10321 tcaatgtgat tgtgttgcat taaatgcttt acaccttgat acatttgatt aataatgtct
    10381 tcttttcgtg ccaacatatt ttcaaaatta acattagcat ctttgacatc aacgccaaac
    10441 attgctgcct gttttactgt ttgaaatact tcagcagatt taagcagcga tttagtagga
    10501 atacaacctt tatggagaca agtacctcct aatagttgtc gttctactat tgccactttt
    10561 ttacctaatt gagacgcacg tatcgcagca acatatcctg cagtacctcc accgagaacg
    10621 actaaatcat attgtttctc tgacatgttc ttactcctaa ctaatgatat atatccattg
    10681 aaaatttatt aatacatagt tttcatgtcc attaattacc tattttacat gattgtctat
    10741 ttagtttgaa tgcacataaa taaatccata aatgagtatt caacacactt tggaaattat
    10801 ctatgtcttt gattgtgttg tatcatttct cttgcatttt cacgagttaa atcagtaaca
    10861 cttgcacctg aaatcattcg tgcaatttca tctactttat catcgccaat taactcttga
    10921 acttgtgttg ttgtacgatc atcttttgat gatttcgaaa ttaataaatg atggtcgctc
    10981 atcgatgcaa cttgtggcaa gtgagatata caaataactt gtatatattc tgctatatct
    11041 cgcattttct ctgccatttt ttgtgcggct tgtccagata cacctgagtc aacctcatcg
    11101 aataaaattg cagtttgacc tctcgattta acaaaaatac tttttaacgc taacataatt
    11161 ctagaaagtt ctccacctga cgcaatttta tttaagcttt ttaatggttc ccctttatta
    11221 ggactgatta aaaattctac aaattcgatt ccatcaatat tcggttcttc taatttttta
    11281 aatgaaatct caagatttgc atctttcatt tgtaagtttt gaatttccga tacaatgtgg
    11341 tctcttaact ccctagcaac tatacgacgc tgcttagata acgcttgtcc aacctctata
    11401 acttgattat acaatgcatt tatttcttct cgtaactgcg atgtactttg ttcatagttt
    11461 tcaattttat tgatttcatt attaagtttt tcttgatatg cgattaattc tgaaatatct
    11521 tttccatatt tgcgttttaa attattcagc aaattcatac gagattcata ctcatttaac
    11581 acttgttcgt cgaattctgt attagccatt tcatcatata actcatgttt tgcatcttct
    11641 aaaatgtaat aaaattgatc aatatcttct tttaatttgt catatttgtt tggaactata
    11701 tcgtttattg ttaacaaatg gttgcttagt tcatacaaac gatcagtgat agcattttca
    11761 tccgttaatg tcatatgtgc gttattaaga gctaagctta atttttcaga gttttgaatg
    11821 cgtttaatat ctatttcaag ttgctctatt tcgccttctt ttagatgtgc ttcagacaat
    11881 tcttctaatt ggaatttcat taaatctaaa cgctgcagca atgcttggtc tgctgattct
    11941 aaatcttcta acccttgctt tttggcttta taattttgaa aagtttggtg atatttatcc
    12001 aacaaatctt gataacgtga ttctgcgtaa ttatccaata atgttaaatg gtatttttgt
    12061 ttcaacaaag attgcgtttc atgttggcca tgaatatcta ataattcttg cataactttt
    12121 cgtaaatctt gtaaagtaac tgtttgatta ttaattttac aaagactttt accagagctg
    12181 aaaatttccc gtttaactaa taaaaaatct tcatctacat caatatccat atttttcaat
    12241 atatgtatag catctttact ctcgtcaata tcaaatatac cttcgatgac agcctttttt
    12301 tcaccatgtc ttacaaaatc agatgaagct ctcattccaa ttaattgtcc aattgcatct
    12361 ataataattg acttacctga acccgtttca ccacttaaaa cagttaaacc atcagaaaat
    12421 tgaatttcta actcttcaat aatagcaaat tgcttgattg ataaggtttg taacataaac
    12481 tcatcgcatc cttataacaa attgaaaatt cttgacttga tttcatcact tgcctctttg
    12541 cttcgacaaa taattaaaca agtatcatca ccacaaattg tgcctagtac ttcttcccaa
    12601 ttgatttgat ctaatatagc tccaatagat tgtgcattac caggtaatgt ttttagaaca
    12661 agtaaattat cagtaccatc tatattaaca aaggaatcca ttaaataacg tcccaatttt
    12721 tctaaaggat ggaattttct atcatttggt aaactataaa catattgacc tgaaggtata
    12781 ggtactttaa taagttgtag ttctttaata tcacgagaaa cagttgcttg agtgacattt
    12841 aaatcataat cgtttaatcg tttaactaat tcatcttgtg tctctatctg ttcatttgaa
    12901 ataatttctc taatttttat atgcctaacc gattttttgg gcacagtaaa cacctctgat
    12961 tacgaatatt tatacattta ttttaacaca tgcactgatt tacgactact aaacaccttt
    13021 ac
//
LOCUS       DATTQF010000068        12889 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.068, whole genome shotgun sequence.
ACCESSION   DATTQF010000068 DATTQF010000000
VERSION     DATTQF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.068"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..139)
                     /locus_tag="VB241_001595"
     CDS             complement(<1..139)
                     /locus_tag="VB241_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244888.1"
                     /translation="MKRIVLFGKVIVDCPIWLNPVDYLVKSVLLKSIKLRFQEVRKFE
                     IL"
     gene            complement(269..772)
                     /locus_tag="VB241_001596"
     CDS             complement(269..772)
                     /locus_tag="VB241_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP1244889.1"
                     /translation="MSKWIVVSDNHTESGILYQIYEAHPDADMYLHLGDSEFEYDDTE
                     LSLFRRVKGNCDFYPEFENEAVAKCNDVRAFYTHGHLYQVNRTRYLLAEQARELGCVF
                     AFYGHTHVAKYEYINGVHAINPGSISQSRSSMEESYAEVLIDDQTLQGTVNFKNRQHE
                     TISHTTF"
     gene            complement(765..1352)
                     /locus_tag="VB241_001597"
     CDS             complement(765..1352)
                     /locus_tag="VB241_001597"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEP1244890.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELILDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DRTMAQLSKEQKGQISHRRNAINLLEAYLAGDQNV"
     gene            complement(1364..2164)
                     /gene="racE"
                     /locus_tag="VB241_001598"
     CDS             complement(1364..2164)
                     /gene="racE"
                     /locus_tag="VB241_001598"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP1244891.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDTTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2403..3218)
                     /gene="sdhB"
                     /locus_tag="VB241_001599"
     CDS             complement(2403..3218)
                     /gene="sdhB"
                     /locus_tag="VB241_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEP1244892.1"
                     /translation="MTEQSVKNTPQHEIQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3218..4984)
                     /gene="sdhA"
                     /locus_tag="VB241_001600"
     CDS             complement(3218..4984)
                     /gene="sdhA"
                     /locus_tag="VB241_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEP1244893.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5036..5650)
                     /locus_tag="VB241_001601"
     CDS             complement(5036..5650)
                     /locus_tag="VB241_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HEP1244894.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5974..7755)
                     /gene="uvrC"
                     /locus_tag="VB241_001602"
     CDS             complement(5974..7755)
                     /gene="uvrC"
                     /locus_tag="VB241_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP1244895.1"
                     /translation="MEDYKKRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7921..8016
                     /locus_tag="VB241_001603"
     CDS             7921..8016
                     /locus_tag="VB241_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244896.1"
                     /translation="MFDYHLTFIDLLIPPFSSRKQYDDLSFNFIY"
     gene            complement(8079..8393)
                     /gene="trxA"
                     /locus_tag="VB241_001604"
     CDS             complement(8079..8393)
                     /gene="trxA"
                     /locus_tag="VB241_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP1244897.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8566..10914)
                     /locus_tag="VB241_001605"
     CDS             complement(8566..10914)
                     /locus_tag="VB241_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP1244898.1"
                     /translation="MRQKTLDVLEFDKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARCSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKNADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEDAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10924..12636)
                     /gene="polX"
                     /locus_tag="VB241_001606"
                     /pseudo
     CDS             complement(10924..12636)
                     /gene="polX"
                     /locus_tag="VB241_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
     gene            complement(12709..>12889)
                     /locus_tag="VB241_001607"
     CDS             complement(12709..>12889)
                     /locus_tag="VB241_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP1244899.1"
                     /translation="SRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHS
                     LIFHIPYLSAFTINL"
BASE COUNT         3892 a         2506 c         1797 g         4694 t
ORIGIN      
        1 ctaaaatttc aaatttcctg acttcttgga atcttaattt tatacttttt aataatacac
       61 ttttaaccaa ataatcaaca ggattcaacc aaataggaca atcaacaatc actttcccaa
      121 aaagtacaat tcttttcata acataaatgt gttattcata ccagttacct tctaaaaatt
      181 gtcagcaaca cttttaaaca aacattcatg tcacgacctc ctagttaaat cagtctataa
      241 actaaaaaaa gccatagcta cttttaattt aaaaagtagt atgactaatt gtttcatgtt
      301 gtcggttttt aaaattcact gtaccttgta atgtttgatc atcaatcaaa acttccgcat
      361 acgattcttc cattgaactt ctagattgag atatacttcc aggattaata gcatgaacac
      421 cattaatata ctcatatttt gcaacatgtg tatgtccata aaaagcaaat acacaaccta
      481 attcacgtgc ttgctcagct aataaatatc ttgttcgatt gacttgatat aaatgtccat
      541 gagtgtaaaa tgctctcacg tcattacatt ttgcgaccgc ttcattttca aattctgggt
      601 aaaaatcaca attgcctttt accctacgaa ataagctaag ttctgtatca tcatactcaa
      661 attctgaatc gcctaaatgc aaatacatat cagcatctgg gtgtgcctcg taaatttggt
      721 ataaaatgcc agattcagta tgattatcac tcacaacaat ccatttagac attttgatca
      781 cccgcaagat aagcttctaa taaattaatt gcatttcgtc tatggctaat ttgacctttt
      841 tgttctttag aaagttgcgc catggttcta tctaatttcg gtacataaaa tatcggatca
      901 tatccgaaac cattttcgcc atatttccca tctgcaattt cacctgaaac agtaccttta
      961 aatacttttg tttccatatc agggccactc atacttatga cacaaacaaa ttgtgcacga
     1021 cgatcagttg tattaccaag cttatttaat aatttttcaa tatttgcttc atcgctttta
     1081 ttttcaccag cataacgtgc agagtatata cctggctcac catttaatgc aaaaacttct
     1141 agtccactgt catcagctat gaccgtttta ttcaatgctt ttgcagcagc ttctgatttt
     1201 aatatagcat tttcttcaaa tgttgatcct gtttcttcca catcaaaatc tagtattagt
     1261 tctgaaatac ctattacgtg gtaatctgga aatatcactt taaagtcatt tattttccct
     1321 tgattattcg atgcaataac aatctctttc attaaaaatc ctcctagtca ttcactgata
     1381 tacgttccac attgacagat aaatttagcc attctttgat aatgttagta atatgtgtgg
     1441 tatcacctgt tgcaaaaaat cgatgatctg gatgttcagt ataacttgca tgttcattac
     1501 taaatgttag caatgcacta acttcacgag ccgtttctaa tccagacgaa atcactgtct
     1561 ttttaccacc aaaataatca tagataggtt tatagagcaa tggatagtgg gtacatccta
     1621 aaatgacagt atcagactca ctattacgcc aacgtttcag tgtttgatga ataacaatgc
     1681 ttgtaattgt tggatcacta tatctcattt gttctacaag tggcacaaaa cctggacagg
     1741 caacgccatg tacctctaca tgtggattta tacgtttaat atgtgttcga tatgcttcag
     1801 atttaattgt gccttccgtt cctagtacta atacattttg atttctagtc gtcattattg
     1861 ctgttctagc acctggttca attacgccaa tcactgggat tgataaggtc ttttgtaaat
     1921 attctaaagc tacagcagtt gcagtattac aagcaatcac gagcattttt atatcaaatt
     1981 ccattaattt acgagcgatt tcaactgtat attgttttac ttgttctcct ggtcttggcc
     2041 catatggaca tcgcccaata tcacctaagt aataaatcgt ctcatttggc aactgacgca
     2101 taatttcttt agctactgtc aaacctccga caccagagtc tattacacct attggtttat
     2161 tcatattcgg tcatccttac ttttaattta atttttagtt tatcataact aagcattgga
     2221 ttttagtatt atgcactgtg tttaccattt ttgtcattat aatataatac ttattttaaa
     2281 ttagctcact atcatattgt catgtaatgt aactataaaa aatcccttct aagtttttgt
     2341 gtatctccat tcaatttaat tttgaaagga acataacatt ttaactcaaa agggattaat
     2401 ttttattcta cttcatggtc tgaaccaaag aatgatttaa acatgtggaa tgttgtttct
     2461 ctgttcattg ctgcaatgga tgttgttaat ggaatacctt tagggcaagc attaacacag
     2521 ttttgtgaat taccacattg ctgtaagcca cctgtaccca ttaatgcatt taaacgttca
     2581 tctttagtca tagatcctgt tgggtgcaaa ttaaacaaac gaacttgcga gattgcttgc
     2641 gcaccaacga atttattatt ttcagtaaca ttaggacaaa cttctaaaca tacaccacat
     2701 gtcatacatt tagataattc ataagctgtt tgacgttttt tctctggcat acgtggcccc
     2761 ggacctaaat catacgttcc atcaattggg atccatgctt tcatacgttt taagttatcg
     2821 aacattctag aacgatcaac ttgtaagtca cggataactg ggaaagtatt cattggctct
     2881 aaacgaatag gttgttctaa ttgatcaaca atcgcagaac aagattgtct tgcacgacca
     2941 ttgataacca tagaacatgc tccacatact tcttctaagc agttcatatc ccagacaaca
     3001 ggtgttgttt tttcaccttt aatattaact gggttacgtc taatttccat taaacatgca
     3061 atgacgttta aattttcacg atatggaatt tcaaatgttt cttcataagg cttagaatca
     3121 cttgtatctt gtcgtttaat aattaatttt actgtttttt gtttcggttt agattgtatt
     3181 tcatgttgtg gagtgttttt cactgattgt tcagtcatta ttttttaccc cctttagact
     3241 tacttgtgta atcacgttta cgaggtggta ttaaactcac atcgacgtca tcataagtaa
     3301 actgtggttt ttcaaatgcg ccttggaatg aggccattgt cgtttttaac cactcttcat
     3361 cattacgctc tgggaattct ggtttataat gggcaccgcg tgattcgtta cggttatatg
     3421 caccaatcgt aatcacacgt gcaagtacta acatgttcca taattgacga gtaaagaata
     3481 ccgcttggtt actccaagtt tgagtatctt ccatatcaat atcttcataa cgtttcatca
     3541 attcaacaat ctttttatct gtttctaaca gtttttcatt ttcacgaaca acagttacat
     3601 ttgctgtcat aatttcacca agttcacggt gtaatttata tgcattttct gtaccgcgca
     3661 tagctaataa tttatcaaaa cgttcttgct cttcagcttt acgtttttca aaaatacttt
     3721 cgtccatatc agtatatgat cgatcaatat ttgaaatata atcaatcgcg tttggacctg
     3781 ctactgtacc accataaatc gctgataaca atgaattggc acctaagcgg ttaccaccat
     3841 gttgagagaa gtcacattct ccagctgcaa ataacccttt aatatttgtc atttgatcat
     3901 aatctacata tagaccaccc attgaatagt ggacagctgg gaaaatcttc attggtactt
     3961 tgcgtgggtc atcaccagtg aatttttcat aaatttcaat gataccacct agttttacat
     4021 ctaactcatg tggatcttta tgtgacaaat caagatatac catgttttcg ccattaatac
     4081 ctaatttttg gttaatacat acatcgaaaa tttcacgcgt tgcgatatca cgaggtacta
     4141 agttaccata atcaggatat ttctcttcta agaagtacca aggcttacca tctttatatg
     4201 tccaaattcg tccaccttca ccacgtgctg attcactcat tagtcgtagt ttatcatcac
     4261 caggtattgc agtaggatga atttgaatga actcaccatt agcataaata gcgccttgtt
     4321 ggtaaacaat ggaagccgct gatcctgtat taatcattga gtttgttgtt ttaccgaaaa
     4381 taataccagg gccacccgtt gccataataa ctgcatctga accaaatgtt tcaatctcag
     4441 cagttgtcat attttgtgca acgatacctc ttgcactatc atcgtcacct ttaactatgc
     4501 caaggaattc ccatccttca tacttcgtaa ctaatccatc tacttcatat gcacgaactt
     4561 gttcatccaa tgcatataat aattgttgtc cagttgttgc ccctgcatat gctgttctgt
     4621 ggtgtaatgt accaccgaaa cgtctaaaat ctaatagacc ttcatttgtt ctattgaaca
     4681 ttacgcccat acggtctaat aaatgaataa ttttaggtgc tgcctctgtc atcgctttaa
     4741 caggtggttg gtttgcaagg aagtcgccac catacactgt atcatcaaag tgaatccaag
     4801 gagaatcgcc ttccccttta gtattgaccg caccattaat gccaccttgg gcacaaacag
     4861 agtgcgaacg ctttactggt acaactgaga acaaatctac atgtgcacct ttttctgccg
     4921 ctttaattgt tgacattaag cccgctaggc cacctccgac aacaataaga tgtttctctg
     4981 ccataaaaat ttcactcccc taaattttca atctatattt gttaaatgcg atgtattaca
     5041 taaaggcaat aattgcagta acaccaatat acgaaataac taagaatacg attaatgaaa
     5101 cccatgtaaa tactcgttgt gattttggag attgaagtcc accccaagta actaagaatg
     5161 accataagcc atttgcaaag tggaacacaa cagcaataat acaaataata taaaatattg
     5221 cccatccagg atgttgcaat gtttcatgca ttaaatcgta attcacttct ttgccgtaaa
     5281 atgctttttg taaacgtgtt tgccataaat ggataccaat aaagataaat gttaagatac
     5341 cactcactct ttggaagaag aacatccagt ttctaaaaat cgagtaatgt ccaacatttt
     5401 cttttgctgt aaatgcaatg tgtataccaa acaaaccgtg atataacaac ggaatgtata
     5461 taaataaaaa ttctacaata attagaaatg gtaatgattc cataaagtta gatgctttat
     5521 taaacgcttc agcaccttgt gttgcttggt gattcactaa taaatgaacg accaaaaatg
     5581 cacctattgg gataatacct aataacgagt gaatacgtct tagataaaat tcattttttg
     5641 attgagccaa aaggagtccc ccctgtgaac gaatatttaa tttattgagc tatttatatt
     5701 aaacgtacgc ttaaccccct aaagtgatat cgtttctagc gttgtcatta tcattaagcg
     5761 aaacatttta aagacaaata cacactgcac gatcaccaaa ctgcatgtcg aacaatgtaa
     5821 aatttggatt taatatttaa gattgcttgt gatgataaac tttctcattt agaaaacgct
     5881 tccacgtaca ttcaaaaaaa taactttgtt aaccatattg taacattatt tcatatattt
     5941 tggggcatga gaatgattct cacgccctgt aatttattta tgcaattgtt catgtaggtt
     6001 ctttgcaacg ttttcaggaa tacctatatt tttaaaatct tcaagtgtag cttccttcat
     6061 tttcttgatt gaaccgaatg aacgcaataa taatgtttta cgtttgctac cgataccatc
     6121 tatatcatca aggattgatt tcaagcctgt cttttgacgt gtttgtctat gaaatgtgat
     6181 tgcgaatctg tgaacctcat cttggatacg gtgcaacaaa taaaatgctt ggctattttt
     6241 cttcagtggt acaatttctg cactagcgcc atataataat tcagatgttt ggtgtttatc
     6301 atttttctgc aaacctgcaa cagggatatc aagacctaat tcgttttgta gcacatcaat
     6361 aaccccgttc atatgtcctt taccaccatc tactattatt aaatcaggta atggtaatcc
     6421 ttcgtttaaa acgcgagaat atcgtcgtct tactacttct ctcattgatt tgtaatcatc
     6481 tggaccttta accgttttga ttttatactt tctataattt ttcttgtctg gtttaccgtc
     6541 gacaaatgta accattgctg acactggatc cacaccttga atattagaat tatcgaatgc
     6601 ttcaattcta attggtgttt gaattcccat ttgtgttcca agttcttcaa tagctttaat
     6661 tgttctggac tcatcacgtg atattaattc aaatttatta tttaaggata ctttagcgtt
     6721 atgtgcagct aagtcaacca tatctttttt gggacctcgc gcgggttgaa cgattttagt
     6781 gtccacaaca gattgaatca tttctttatc caaattacgt ggtacatgaa cttccttagg
     6841 taaaatatgt tggtttaagc tataaaattg tccaataaat gtataaaatt cttcttcttc
     6901 tgtttgctgt aatggaatca tcgttgtatc tcgctttatc atattacctt gtcttataaa
     6961 gaaaacttgg atacacatcc atcctttatc aacactataa ccaaagacat cacgaatcgt
     7021 tttatctgat gacataattt tttgtttgtt agttagattt tgaatatgtt gaattaaatc
     7081 tctgtattct ttagctcgtt caaaatcaag tgattcactt gcagttaaca ttctctcttc
     7141 taaacttttt aaaattgttt tgtcttcccc attcagaaaa tcagtaattt ccttcgtcat
     7201 ttgtgcgtat ttactcaaat caacgtcata tacacatggt cctaaacatt gtccaatatg
     7261 gtaataaaga cataatttat ctggcatctt atcacatttg cgatatggat atattctgtc
     7321 taataacttt ttagtttctt gagcagaata tgcattcgga tacggtccga aatatttacc
     7381 agtaccttgt tttacagttc tcgtcactag tagtctagga tatttttcct tcgtaatttt
     7441 aataaatgga taacttttat catcctttaa taatatatta tatcttggtt gatattgttt
     7501 aatcagattt aattcaagta aaagtgactc tgtttcacta gacgtgacaa taaactcaaa
     7561 gcgacgtatt tcaccaacca gtctcgttgt tttagcatca tgagcgcccg taaaatatga
     7621 tcgcaatcga tttcttagct ttttagcttt gccaacatat atcacttgat cgttacgatc
     7681 tttcattaaa tagcagcctg gttccatagg tacgacattt aatttatttt taattcgttt
     7741 cttatagtct tccaaaaata tccccccttc taattttaaa atgaatgatg ctacgaaagc
     7801 gcttcaaatt tatcaccgct aattctagtt cataatcaac atcttcgagt acgacttttc
     7861 atgcacatca tgtaatattg aacattgttt tatcaaacca actattacat atctatcagc
     7921 atgtttgatt atcatttaac gttcatagat ttattaatcc caccattctc aagtcgtaaa
     7981 caatatgatg acttatcatt caactttata tattaaaaac acaaacaaaa actggagcaa
     8041 ttcattaaag aaatgcccca gtcgtcattg gttgtaactt ataaatgttt atctaaaact
     8101 tcagctaagt tttcttttgg ttggaaacca acaactttat caactggttg accgtcttta
     8161 aagacgatta atgttggaat actcatcact tcatatttag ctgcagttga tggattttca
     8221 tcaacatcta attttaaaat gtcagcttta ccttcatagt cagctgctaa ttcttctaat
     8281 accggagcga tcattttaca tggaccacac catgttgccc aaaaatctac taattgtaca
     8341 ccagattcta cttttgaatc aaaatctgca tctgttactt taacgattgc cataaatgcc
     8401 aatcctcctt aattattatt atgatgttca aattatatca gataacatgc ttcatgtatt
     8461 tctatttgct caatttttat gcttatttca aataacatgt ttgaaatgta ttcttgttgc
     8521 tttacattat tttaactgca gctaattaat ttatcaaatt ataatttatt ttagtgttgc
     8581 aacggtaacg ccaaatccac cttcgcttgg cataccacct ctaaagtcac taacactttt
     8641 atgctttttc aaatgttgtt gtacaccttt ttgaagtgca cctgtacctt taccatgaat
     8701 gatataaact tgttcgtaat tacttaaaac ggcttgatct aaatattgat ctagttcaat
     8761 taaagcatcc tcataacgat agcctcgtaa gtcaagttca gttttaattg tttgacgatt
     8821 ttgacgtgta accattttcg ttggtttaac tttttctttt tgttttttct ctaaatcttc
     8881 aataggtaac ttcattttaa taattcccat ttgaacaatt gcttcttcat cattgacaat
     8941 ttctaaaact tcaccctttt gaccgtaaga taatactttt acttcatcac cagcaacaat
     9001 tttatcgtat ttttgctttt gtacattttg ctttatagat ttcgcttcat aatgatcatc
     9061 taatcgtttc ttcttatcaa tcaattcatg ttctttaaca tctgcacctt tttgttcacg
     9121 caattgtctt aggtctttaa taatatcgtc agcttctttt gttgctgctt taattttttg
     9181 attcgctttt tctttagcat cttcaattag agatttttca taattttgga attgttgata
     9241 ctgcttagat aaatcatcat gcacttgctc cgcttctttt acaagacggt ccagttctaa
     9301 cctctgtgtc tctacacgtt tgtaattacg ctctaatgat tcaatcattt catttatttc
     9361 tttttcatca gtaccaatca tcgtcttagc cttattaata atattcaaac taagacctaa
     9421 ctttttagaa atatcaaaag catttgaacg acccggcaca cccattaata acttatacgt
     9481 tggactcaat gtatctacat caaattctac actcgcattc ataacgcctt ctcgattata
     9541 actatatgct ttaagttcag gatagtgcgt cgttgccatt actagagaac caatttttct
     9601 aacatgatct aaaatgctca ttgctaatgc agcaccttca cttggatctg tacctgcacc
     9661 taattcatca aataaaacta aactatgttt gtctgcattc tttaaaattt caactatatt
     9721 cgtcatatga gatgaaaaag ttgataatga ttgttctatt gattgttcat ctccgatatc
     9781 gcaatatacg tttttaaata cactcaactg actaccatca agtgttggaa tcaacaatcc
     9841 tgattgagcc ataacaataa ttaaacctaa tgtttttaat gttactgttt taccacctgt
     9901 attcggtcct gtaataatta ccgtttcaat atcttccata aattcgatgg tattagctac
     9961 aacagtctca cgatttaata atggatggta tgctttaggt aaatatacag tacgttcctc
    10021 tttaaatatc ggctttgttc ctttaatact tctactacat ctcgcttttg cgattaaaaa
    10081 atctaactga cccatgactt gttctgccac aagtagtgca tctttgtccg cagcaacata
    10141 accagttagt tgcgttaaaa tgcgttcttt ttcaattgct tcatcatgac gtaatcgact
    10201 aatttgatta ttcatttcaa caactgatga tggctcaata tacaatgttt gtcctgaagc
    10261 agattgatca tgtacaatcc cattaaaatc ttgtcgatat tcagctttga caggtataac
    10321 gtttctttca ttcctaactg ttacaatagc atctgataat tttttctgat ttgcttggct
    10381 tttaacaata cggtccaaat tttgtctaat acgttgattc gtgctagaaa ttttacttct
    10441 aatcccttgc aattcataac tcgcattatc atataaatca tacgtatcgc atgtttcatt
    10501 tatttgttga aaaagatcag ttaacacagg taattgattc atcttgtcat ctaatattgg
    10561 gtatttaaca ccttcatctt cttcaaccaa ttgattataa aatgtcttaa attgattttg
    10621 tacttgaatt aatcttttta tcaagttaag ctctgataca tttaaaacgc cgccaatatc
    10681 agcgcgatga atgaatgctg atactttaga taagccactc aagcttggta aacgatgctt
    10741 attatagatt tgagcaatct catccgtttc ttccatttga aaaacaaccg tttcaaaatt
    10801 agtagctggc atcatttgat tgaccttttc caagcctaag tcactaattg tttcattggc
    10861 aacgagtgat tttattttat caaattctaa gacgtctaat gttttttgtc tcataaaatc
    10921 cctctatttc ttaagtttta tattattttc aataaagtct ttaaaagctt cacgcgataa
    10981 tgcgttaatc actctatctt ttgttacaaa tcctttttgc gcagttgcta cgccataatt
    11041 cataaaatct aaatgatttg tatgatgcgc atcagtgtta atagttaatt tcacatttgg
    11101 atatttacga acgatatcag cgctcaaatc cagtcgatgt ggattggcat taatttctaa
    11161 tactgtattc gtttcttcag ctaatgccat taattgttca atattcggtt tataaccatc
    11221 tcttctacct ataatacgcc ctgttggatg cgctatatgt cgcacgtatg gattgcgaca
    11281 tgcattagct aatcgttcca taatttgttc ttctgattgg ttaaagcttt gatgaatagc
    11341 tccaattacg taatcaagtt gtgctaaaat ttcatcatca taatccagcg agccatcagg
    11401 taatatatcc atttctgtac ccgaataaat atcaatttca ctatattatt tatctaaagc
    11461 tttaatttct tcgttttgtc ttaaaagtct ttctacttgt aagccattag caacacgtaa
    11521 actttgtgaa tgatcagtaa ttaccatgaa tttataacct tttgcgatat ttgcttctac
    11581 catgtctcga atggaaaacg caccatcact aaacgttgta tgcatatgaa tatcaccatt
    11641 aatatcatct aatgtaatga tattacttag atctttatca aattcgctac catcttctct
    11701 catagcaggt ggtataaaat tcacattaaa atgttcatat attttggctt cactatcata
    11761 ttgaattaat gtaccatcag cttgttcaat tccatattca cttacttttt catcccgtgc
    11821 tttagcaagt tgtcgaattc ttatattatg ttcttttgac ccagtaaaat gctgcaatgt
    11881 atgataaaaa gcccttggtt caattaatcg aaaatcgaca ccaatcgttt catcatcata
    11941 cgctaattct aatgaaactt ttgtgttccc cactgcaact tcttttactt tattgggaat
    12001 atttaataat tgctgctgca ctgcttttgg gttatcggta cttattatga aatctaaatc
    12061 tttgctcatt tctttaaaac gacggaagct tcctgcagat gaatattgat cgatataatt
    12121 taatgtatct atataatcaa tgatttcttg attaagtctt ctcatttgat caattggata
    12181 tctatctttc ttagcaccaa gttgtttcac agcttctaat atgttttgtt ccgttttctt
    12241 agcaaatccg cttaattcac taacttttcc attttcacaa gcaacttgaa gtgaagcttt
    12301 atcaacaata ttcaactctt tatatagctt agcaattttc ttgcttccaa gtccttgaat
    12361 tttcaaaagt ggaataagac cttccggaac ttcttcctgt aattgctgta aatactgaga
    12421 ttcaccggtc tcacggtaat cattgattac ttctgcaaca cctttaccaa tgccttttaa
    12481 ctccgttaca tcagatattt catctaatgg tcgttcatct aattcaagac tttgagctgc
    12541 ttttcgatac gctgatattt taaaagtatt ttcccctttt aattccatat aagtagcaat
    12601 ttgttctaat agtttgataa catctttttt tgtcataata acactccata aaaagaagac
    12661 caggacgtat cattaatata tacctttgtc ctgacctctt atgataattt ataaattaat
    12721 ggtgaaagcc gataaatatg ggatgtgaaa aatcaatgaa tgactcacaa tagatatttt
    12781 aagctgatgt tgtaatgctt cgttaggata taatgccaat agatataacg taaagtgtaa
    12841 gacaattatc gtcataaaca caccaactat cattcccatt gcacgactg
//
LOCUS       DATTQF010000069        12865 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.069, whole genome shotgun sequence.
ACCESSION   DATTQF010000069 DATTQF010000000
VERSION     DATTQF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.069"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(107..943)
                     /locus_tag="VB241_001608"
     CDS             complement(107..943)
                     /locus_tag="VB241_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEP1244900.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(943..1557)
                     /gene="ureG"
                     /locus_tag="VB241_001609"
     CDS             complement(943..1557)
                     /gene="ureG"
                     /locus_tag="VB241_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEP1244901.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(1570..2259)
                     /locus_tag="VB241_001610"
     CDS             complement(1570..2259)
                     /locus_tag="VB241_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEP1244902.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNNPWIAWYHQQMQDKKAKLNPAICFTMLGHYLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2252..2704)
                     /gene="ureE"
                     /locus_tag="VB241_001611"
     CDS             complement(2252..2704)
                     /gene="ureE"
                     /locus_tag="VB241_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEP1244903.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIVVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(2717..4432)
                     /gene="ureC"
                     /locus_tag="VB241_001612"
     CDS             complement(2717..4432)
                     /gene="ureC"
                     /locus_tag="VB241_001612"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEP1244904.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFPNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDYEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4429..4839)
                     /locus_tag="VB241_001613"
     CDS             complement(4429..4839)
                     /locus_tag="VB241_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEP1244905.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGTENVNKKGGKRS"
     gene            complement(4853..5155)
                     /locus_tag="VB241_001614"
     CDS             complement(4853..5155)
                     /locus_tag="VB241_001614"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEP1244906.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5415..6320
                     /gene="yut"
                     /locus_tag="VB241_001615"
     CDS             5415..6320
                     /gene="yut"
                     /locus_tag="VB241_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEP1244907.1"
                     /translation="MKMIDVLLKNISQVVLISNKWTGLFILIGLFVADWTVGLAAMIG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHVIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            6535..7689
                     /locus_tag="VB241_001616"
     CDS             6535..7689
                     /locus_tag="VB241_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEP1244908.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIGDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKEYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELKSVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKASNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYTAESQIRNATSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            7902..8810
                     /locus_tag="VB241_001617"
     CDS             7902..8810
                     /locus_tag="VB241_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1244909.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDIEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9209..10150
                     /locus_tag="VB241_001618"
     CDS             9209..10150
                     /locus_tag="VB241_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEP1244910.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSKEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     RDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10271..10825
                     /locus_tag="VB241_001619"
     CDS             10271..10825
                     /locus_tag="VB241_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP1244911.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSTGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            10897..11667
                     /locus_tag="VB241_001620"
     CDS             10897..11667
                     /locus_tag="VB241_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1244912.1"
                     /translation="MSHISMSDINCDGTIFENNDKQLIYLTPIFPQRYTSNTWLYKKM
                     PTQEQWLKDLKRQHQLHTNQGSNHYAFSFPENEQLDDTWMAIFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGRDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTMQSLVGEYALSRNH
                     KPVILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(11838..12635)
                     /gene="fdhD"
                     /locus_tag="VB241_001621"
     CDS             complement(11838..12635)
                     /gene="fdhD"
                     /locus_tag="VB241_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEP1244913.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAVAKTSMSKITLTPQQIINMMTRLQSASHIYQETGGLHNAA
                     LSDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
BASE COUNT         4303 a         2004 c         2268 g         4290 t
ORIGIN      
        1 ttaacttaaa atttaaatat tgaagatgct ttaattaaag ttaaagacta gccatacctt
       61 atttcagctt attaagcttg aaacaaggta cactagtctt tttattttaa tattttctta
      121 gaaaatcaag tttacgatca taaatatttt ctgcgatata gctttggatg gttccaagta
      181 ttttctctat aatttgtgtg cgataagcaa aaattctaac tgcaaaacca tgtgtaggca
      241 attgagaaat agcaacacga caatcgaatg tattgctgta agaactaatg gtttcataaa
      301 ctgaatcgat tagtttttgg ttaacatctt cgtgtatgaa gtaagcagag ccataatgag
      361 agtaatgttc catataacct atttcattga tcgattgttt attaggattc aataataaat
      421 tatcatatgt gactaactca tcttcaatat aaatttcatt tattaaatgc atatattgat
      481 acttgaaggc ttcgccagtt tttgaataac caggagttaa aatatcagta taaaataatg
      541 aactagaatt attgagattg aacgtattgt gttgataaaa tttagcattt tcatatgcga
      601 tgattggatc agcgacatat tctaaatatg cgttatcttt caaattaaaa gtttgatact
      661 gctcaacatg attggaaggt gtcttgtata tttttgttgc accttgagat gtcaatgtca
      721 ctttagcgtt atcttcgaca ttcacattca tacggtaacg atctccatct aaatagccac
      781 cacctacatt tactatataa aatgtaggaa tggtagattg atttagataa actggacgta
      841 tcactttaag tgctttttca aagaaaatat ctcttgacac tgatcgattg ccatcaaaaa
      901 acactgttaa atcaagttgc ccagtccatt gttgttcatc cattatgata atcctttgag
      961 taaagtgtcg cgttcaatcc aatcgataac ttcatccaaa ccttcgtcgg tttttaagtt
     1021 agtaaaagta aatggacgtt taccacgaaa tactttagta tcttcagcca tttgttctaa
     1081 tgatgcacca acatagggag ctaaatcagt tttgttaatt acaaagaaat ctgacttaat
     1141 cataccttga ccacctttac gtggaatctt ttcaccttga gcaacatcga taatataaat
     1201 tgaaaagtca acaagttctg gactaaatgt tgctgctaag ttatcgccac cagattctat
     1261 gaaaattagt tctatatcgt catgacgttc taataattcg tctattgctg caaagttcat
     1321 agatgcatct tcacgaatcg cagtatgagg acatccacca gtttcaacac caatgattcg
     1381 actttcaggt agaactcctg aatttactaa tatcttttcg tcttcttttg tatatatatc
     1441 atttgtaata acgccgatgc tcatttcttt tgaaagacgt tttacaactt tttcaattaa
     1501 ttgtgtttta cctgcaccta caggaccacc aataccaatt ttaatcggat ttgccacaat
     1561 tataacctcc tatgatatga aaattctaac attgacattc tcatgcgcca tttgatttag
     1621 ttctaaacca ggcgctgtca tgccaaaatc tgcttctttt aattcgaaaa tctgctttct
     1681 tgtttcttct atataaggaa tcatgtgagt aactatcttt tgaccagcag tttgtccaag
     1741 tggaatagca cgaacagcat tttgagttaa acttgaaaca ttttgatata aatagtaatc
     1801 aataatcgtt tcaatatcta cacctaaata atggcctagc atagtaaaac aaatagctgg
     1861 atttaacttt gctttcttat cttgcatttg ttgatgatac caagcaatcc atgggttatt
     1921 ataaagttct aaagccaatt taaccatgcg agtccccatt tgttttgcac caacacgtgt
     1981 ttctttaggt aagttttgaa caaacatcag cttatctatg tgtaatactt tttgtgtatc
     2041 atcattttcc aatgcatcat aaactaaacg catggctaaa ccatcagaat aggtaagttg
     2101 ctcttgtaaa aacattttta accaagcaat aaaagtatga tcatcatgaa ttatatttcg
     2161 ttgaatatat gtttcaagac caaatgaatg actgaaagca cctgttggaa attgtgaatc
     2221 acagaactga aataatctta agtgtgtatg atcaatcatg agaatgtcct atatgtctaa
     2281 aagccttatt aactttacgg tcttctcgaa catatgggat gcctaaactt tttaataaat
     2341 cttcaactaa ataatcatat tgtactagca tttcagtctc tgtaaattgt gctggcaaat
     2401 gacgatttcc taattgatgg gctatatctc ccatttcttg caatgttctt ggttgaatca
     2461 ctaaaagatc ttctgaatta acatccacaa caatcatatt atggtcatct gcgtataaaa
     2521 tatctccata ttgtaagtca ataggttgtt ttaaacgaat gccaatttca gtgccatgat
     2581 ctgttacgac tctttgaata cgtttaacga gatctgaatt ttcaaggtat actttttcga
     2641 cgtgcttttg tttttctgaa tttgacaaat tggcaatatt gccttggatt tcttcaacaa
     2701 tcattctatg ttcctcctag aataagaagt atctttgagt taatggtaac tcagttgctg
     2761 cattacttgt aattttttct ccatctacat atacttcata tgtttgtgga tcaacgtcta
     2821 atttaggtgt tgcgctgtta tttttcatat ctgctttaga taattgtcta atatttttaa
     2881 ctggacgcac catgcgtttt aaatttaatg cacgattgat accattttca taagcagttt
     2941 tagacacgaa tgtcattgac gtacttgtaa ggtttccgcc gtattgacca tacattttac
     3001 ggtacttcat cggttcagat gtaggtatag aaccatttgc atcaccattt acggcagagt
     3061 taattaatcc gccctttaca actaattcag gtttaacacc aaagaaaatt ggatcccata
     3121 agacaatatc agctagtttg cccggctcga tagatcctac atattcagaa ataccatgtg
     3181 taattgctgg gttaattgta tatttagcga tataacgttt gatacgatta ttatcattat
     3241 gttcataatc accatctaaa ggaccacgtt gttctttcat gcgatgtgct acttgccatg
     3301 ttcgtgtaat tacttcacct acacggccca ttgcctgtga atcggaacta atcatactga
     3361 atacacccat atcttgcaga acatcttcag ctgcaatcgt ttctttacga atacgtgaat
     3421 ctgcgaatgc gatatcttca ggaatagccg catttaaatg gtgagtaatc attaccatat
     3481 ctaaatgttc atctacagta ttatgtgtat aaggcaaagt tggatttgta gatgaaggta
     3541 aaatatttgg aaatgcagcg gatttaatta aatcaggcgc atgaccgcca ccagcacctt
     3601 cagtatggta catatgaagt acacggtctt taacagcagc cattgtgtct tccataaatc
     3661 ctgcttcatt taaagtatct gcatgtaatg caatttgaac atcaaattca tcagcaacat
     3721 ctaatgcatg actcaaagca gatggtgttg caccccagtc ttcatgtact tttaatccaa
     3781 ttgctccggc attgatttgt tcaatgagtg cagttggatt tgttgcttgt cctttacctg
     3841 taaaaccgac attaatcggt aaaccttcgg cagcttctaa cattctatga atatgccatg
     3901 gacctggagt tacagtagtt gctttagaac cttctgaagc accagtacca ccaccaatat
     3961 gagtcgtaat accactttct aatgcgactt ctgcttgttc aggattaata aaatgaacat
     4021 gagtatcaat accaccagca gtgacgattt taccttcagc ggcaatgata tctgttgttg
     4081 aacctataat aatgtcgaca ttatccatta tatctgggtt gccggcatta cctatggcga
     4141 aaatataacc atttttaatg cctatatcag ctttaaccac tttatcgtaa tcgataataa
     4201 cggcattaga aatgacaagg tctgcaacgt tcacatcatc acgtgttaca cgaggatttt
     4261 gcgccatacc gtctctaata gatttaccac caccaaaagt agcttcttca ccataaaccg
     4321 catagtcttt ttcaatttga gcaaatagat tcgtatcacc taaacgaata gaatctccaa
     4381 cagttggacc gtataagctc gtatattgat tttgcgtcat tttaaagctc atgatctttt
     4441 tcctcctttt ttattcacgt tttcagtacc gttatctccg aatacgcctg catattcatc
     4501 attttcatca gttgggcgat agacacgtga ctcatcgata ggaccattga ccataccacg
     4561 aaaaccaaaa attttacgtt tgccagcata ttcaactaat tgaacttctt ttttatcccc
     4621 aggttcaaat cgaacagctg ctccagctgg aatatctaaa tgttttccat atgccatttc
     4681 acgttcgaaa tctaatgctg catttgcttc ataaaaatga aaatgtgagc ccacttgaat
     4741 tggtcgatct cctgtatttt caacttcgat aactgtttca ggatgatggt tattaatttc
     4801 aacctctgta ctttttgtaa taatttctcc tggtatcatt tgactgcctc ctttaaacaa
     4861 tagggtgatg tactgtgatt aacttagtac catcggggaa cgtagcctcg atttcgatat
     4921 ctgtaatcat gtgttcgaca ccatccatga catcttcttt gtttagaatt tgtctaccat
     4981 aactcattaa ctctgcaacg gtcttaccat cgcgtgcacc ttctaataat tcatcgctga
     5041 ttaaagctaa tgcctcagga tgatttagtt tcaaaccacg tgctttacga cgacgtgcaa
     5101 cttccgccgc cactacaatc attaatttgt cttgctctcg ttgtgtaaaa tgcaaattaa
     5161 aacccccaat ttcatattag atacaattta caaaatttat attaatccta attgttgtga
     5221 taaacaagta atatacaaag tttaatacgg aacagcaaaa aataaattta ctatttcgta
     5281 tttaaaaata tatgattatg attaaaacga atagcaaaat atatttagga tttggaaaat
     5341 agagttttta atataacttt ttgtaaaatg gtagtatatt aatgagtaaa cattcaagaa
     5401 aaagggtggg cattttgaaa atgatagatg tgcttttgaa aaatatatct caagtagtgt
     5461 taattagtaa taagtggact ggtttattta tcttgattgg attatttgtg gccgactgga
     5521 cagttggatt agcagctatg ataggtagta tcatagctta tgcatttgca cgttacatta
     5581 attatagtga agcagagatt aatgatgggt tagccggatt taatccagtg ttaactgcca
     5641 ttgcgttaac aatcttttta gataagtcag gattagatat tgttataaca atgatagcaa
     5701 ctttattaac attaccagtc gctgctgcag tgagagaagt tttaagacca tataaagttc
     5761 cgatgctgac gatgcctttt gtcattgtga cttggtttac aattttactt tcaggacagg
     5821 ttaaatttgt agatacatcg ttaaagttaa tgcctcaaaa cattgaaacg gttaatttta
     5881 gcaacaatga tcgaatacat gtcattcagt cattatttga aggattcagt caagtattta
     5941 tcgaagcgag tgtaattggt ggtgtatgta ttttaatcgg catattaata gcatcaagaa
     6001 aagcagcact cttagctgtt atagctagtt tgttaagctt tatcattgta gctctattag
     6061 gtggtaatta tgatgatatt aatcaggggt tattcggtta taactttgta ttaatggcaa
     6121 tcgcactagg atatacattt aaaacagcga ttaaccctta tatttcgact tttttaggtg
     6181 tgttattaac tgtagtggtg caactaggta caacaacatt gcttgaaccg tttggcttac
     6241 ctgcattaac attgccattt attattgtga catggatttt attatttgcg ggtattaaac
     6301 atgacaaagt agatgcttga tagttaaatc aaacctagta ttgtttgaat atcaccttaa
     6361 accatacagc gaattgtata gtttaaggtg tatttttgga gaaaattaat tggatactta
     6421 atcgaattat cagctttctt ttaattgttc acagcacgtt cctttttatt tttaaagtcg
     6481 agtgttaaac taggaataaa taatttatat tgtgtgatgt gtggggtgac taatatgaat
     6541 gatatggata attccttttt aataacaacg gaaattcaaa gaaaatggat tgaaaaattc
     6601 aaagtaattg gagatacatt taaggctaag gctgaatata atgatcaaca tagccaattt
     6661 ccatataaaa atattgaatg gttaattaaa gaaggttatg gaaaattaac gttaccaaaa
     6721 gaatatggtg gtgaaggtgc gaccatagaa gacatggtta ttttgcaatc atttttaggc
     6781 gaacttgatg gtgccacagc attatctatt ggttggcatg tgagtgtcgt aggacaaatt
     6841 tatgaacaga aattatggtc tcaagatatg ttggagcaat ttgctgttga aattaataat
     6901 ggtgcattag ttaatagagc agttagtgaa gctgaaatgg gtagtccaac aagaggggga
     6961 agaccaagta cacatgctgt taaagctgat gatgggtata ttttaaatgg tgtgaagaca
     7021 tatacatcaa tgagtaaagc actaacacat attattgttg ctgcttatat agaagaatta
     7081 aaaagtgttg gttttttctt agtggacagg aatttgcctg gtgttgaaat tgccgataat
     7141 tgggacgtat taggtatgcg agcgacagaa agtcatgatt taatattaaa tgatgtaaaa
     7201 gtaccattaa aacatttagt ggaaacagag aaaagtaaag cgtcaaatgg ttggatattg
     7261 catataccaa gctgttattt aggtatagca caagctgcta gaaattatgc tgtagacttt
     7321 gcaatccaac atagccctaa tagtatagaa ggaacgattg caacattgcc aactgtgcaa
     7381 caaaatttag gaaaaatgga aacgctgttg ttatgtgcga gacaattttt atggagtaca
     7441 gcgaaaggct atcaacaata tacagccgaa agtcagatta gaaatgcgac gagtgcgagt
     7501 aaagtaatgg taatgaacca aggccttgaa gtgattgatt tagcgatgcg cattgttggt
     7561 gctaaaagtt tagaaatgaa tcggccatta caacgttact atcgtgacat gcgtgcagga
     7621 ttacacaatc cacctatgga agatgcagca tatacaaata ttgctaagag tattacagat
     7681 acattttaaa tttaaaaagg taaatcgatc gttgattggc ataaagagat gaaatttgac
     7741 gttcggattt accttttttg cgggaattta taggtatatt aagatatttt gctaaatttt
     7801 ttgcgaaaat aaaaaaataa ataacactag ctattgtaaa tgtatatgca atcttgtata
     7861 attttggaat aattgataat cattttcaat aggaggaaat tatgaaaaaa ctattattac
     7921 cattaataat tatgttatta gtgttagctg cgtgtgggaa ccaaggtgaa aaaaataaca
     7981 aagctgaaac taaatcttat aaaatggacg atggcaaaac ggtagatatt ccgaaagacc
     8041 ctaaacgcat tgcagtagtt gcgccaacat atgctggtgg acttaaaaaa ttaggtgcaa
     8101 acattgtagc tgtaaatcaa caagttgatc aaagcaaagt gttaaaagat aaatttaaag
     8161 gtgttactaa aattggtgat ggtgatatag aaaaagttgc taaagaaaag ccagatttaa
     8221 ttattgtata ctctactgac aaagacatta aaaagtatca aaaagtagca ccaacagtag
     8281 ttgttgacta taataagcat aaatacttag aacaacaaga aatgttaggg aaaattgttg
     8341 gtaaagaaga taaagtaaaa gcttggaaga aagattggga agaaacaact gctaaagacg
     8401 gtaaagaaat taaaaaagca attggacaag atgcaacagt gtcattgttt gatgaatttg
     8461 ataaaaaatt atacacttac ggcgataact ggggtcgtgg tggagaagta ttatatcaag
     8521 catttggttt aaaaatgcaa ccagaacaac aaaagttaac tgcaaaagca ggttgggctg
     8581 aagtgaaaca agaagaaatt gaaaaatatg ctggtgatta cattgtgagt acaagtgaag
     8641 gtaaacctac accaggatac gaatcaacaa acatgtggaa gaatttgaaa gctactaaag
     8701 aaggacatat tgttaaagtt gatgctggta catactggta caacgatcct tatacattag
     8761 atttcatgcg taaagattta aaagaaaaat taattaaagc tgcaaaataa ttcagctata
     8821 taagccagtg aaatgagagt ctgaaacata tcaatctttt gatattgtat taggctctta
     8881 tttttatagc aagaaggtta gatttttgta tttttttaaa taataagtgc cgttattatc
     8941 attcaattta attaatgata gattagtatt attacagcta aagtagtata cctgagaaaa
     9001 tagctcaatg tatctctgca ttaataagtt atatcataat tattttagtg catactttat
     9061 ggaaggcata tcagggaatg cctatcaatt aaagaagagg tttaaaagga ttacaacaga
     9121 atgttatgat tttgtagaaa gatatattac aacgttttat aaaaacataa tattgttaat
     9181 ggaaaatgaa atgtaagggg gatttcgagt gactaagaaa gtttatttta accatgatgg
     9241 tggtgtagat gatttagtat ctctattttt attattacaa atggaaaacg ttcaattgat
     9301 aggggtcagt acaattggtg ctgattgtta tttagaacca tctttgagcg catcagtaaa
     9361 aattattaat cgtttttcaa aggaagatat tcaagttgcg ccatcatatg aacgaggaaa
     9421 aaatccattt cctaaagaat ggcgtatgca tgcctttttt atggacgcat tgccaatttt
     9481 aaatgagcca gtcaaacacg ttgcttcaaa tgtgagagac aaagaagcct ttgaagacat
     9541 tattcaaact ttaaagagac aatcagaaaa agtaacatta ttatttacag gcccgcttac
     9601 agatttagca aaagcactac aaaaagattc atcaatcgtt cagtatatag aaaaattagt
     9661 ttggatgggt ggtacctttt taccaaaagg aaatgttgaa gaacctgagc atgatggttc
     9721 tgcagaatgg aatgcatatt gggatccaga agcggttaaa attgtttttg atagcgatat
     9781 agagattgat atggttgctt tagaaagtac gaatcaagta ccgctaacgt tagatgttag
     9841 acaaagatgg gcgaatgaac gccaatatac gggcattgat tttttaggag taagttatgc
     9901 agcggtacca ccattaacac actttataac aaattctact tactttttat gggatgtttt
     9961 aacgactgct tatattggta acaaggactt agttcactca attgagaaaa aagtggatgt
    10021 aataagttat ggaccaagtc aaggtaagac atttgagtgt aaagatgggc gcaaaattaa
    10081 tgtcataaat catgtagata acaacgcatt ttttgattat ataactgcac ttgctaaaaa
    10141 agtaaattaa cagctgtgta gaataattaa ggttttaatt tatatagaac aacttattgt
    10201 aaacttttcg ttccttaaag tttacaatgg tgctataata atggtcatga aatacgaaag
    10261 gaagtaaaaa atgacaacaa aacagttagt atacacagct ttaatgacag cgattatcgc
    10321 tattttagga ttggtaccgg taattccact accattttct tcagtaccaa ttgtacttca
    10381 aaacattggt attttcttag caggtgtgat tttaggacgt aaatatggca cattaagtgt
    10441 tatcgtcttt ttattattag tagttgctgg tttgccactg ttatcaggtg gtcgcggtgg
    10501 cattggtgta ttcgcaggtc cttcaacagg gtttttacta ttatatccag ttgtagcatt
    10561 catgattggt gcgattcgtg atagattcat caatgaaatt aatttctgga ttttattcgt
    10621 tggtatttta gtttttggtg ttatagcatt agatgttatt ggtacattga ttatgggcat
    10681 gattattaac ataccattta cgaaagctat ttcaatttca ttagcttatt tgcctggtga
    10741 tatattaaaa gcaattgtag caagtttgat tggtacagct ttacttaatc actcgcagtt
    10801 tcgtcaaatt atgggattaa agtaatcata tttaagataa aaaagtaatt gattaagttg
    10861 cttttcaatt gttgaaagtg aaaggagtag gtgccaatgt ctcatataag tatgtcagat
    10921 attaattgcg acggtacaat atttgaaaat aacgacaagc agttgattta tttaacgcct
    10981 atttttccac aacgatacac aagcaacaca tggttatata aaaagatgcc tacccaagag
    11041 caatggttga aagacttaaa gcgtcaacat caattacata caaatcaagg ttcaaatcat
    11101 tatgcattta gttttccgga aaatgaacaa cttgatgaca cttggatggc tatttttaaa
    11161 gatatgaatt ttgaactagg tattatggaa ttgtatgcca tagaaagtga tgcgcttgct
    11221 aatttgccgc gtaactctga cgttgaaatt gctatcgttg acgagtcgca tatagatgcc
    11281 tatttaaaag ttgcatatca gtttagtttg ccatttggaa gagactatgc agatgcacat
    11341 gaaaaaatgg taagggaaca ttatcaaaaa gatgtgatta aacgcttagt agcttattta
    11401 aataatgaac ctattggcgt tgtagatgtc attgaaagtg aaaattacat tgaattagat
    11461 ggatttggtg tactagaaca atttcgacac caaggaatcg gttctaccat gcagtcatta
    11521 gtaggtgaat atgctttatc tagaaatcat aaaccggtta tattagttgc agatggcgaa
    11581 gatacagcaa aagatatgta tgcaaagcaa ggttatgttt accaatcatt ttgttatcaa
    11641 atattaaaag aagatattgg aaattaacga ttgatgatag gtttgtatta gaataaaaaa
    11701 gcagtaaact ggtttcgagt agaaatcaac ttactgcttt ttaaattgtt ttgagctact
    11761 tatacttata aaaatagtgc ttttatattg ttgattcatg tagaatacgt tcattatgac
    11821 atactataat gaatatgtta ttgttcagaa tcaatgatac gttctggatg actgtatata
    11881 ttaaagccac catttcgaat aaatccaact gccgtaatat ttaggtcatt agctaaggtt
    11941 acagcaagcg ttgtcggagc agatttagat aaaatgacgc caacaccaat tttcgcggct
    12001 ttaattaaaa tttctgatga aatacgtcca ctgaaaatta atactttatc tcggacagta
    12061 atatgtcgct gaatacaaaa tccatataat ttatctagag cgttatgtct gccaatgtct
    12121 tgtcgatgta caaaaaatgt caaaccatcg cttagagcag cattatgcag gccacctgtt
    12181 tcttggtaaa tatgactggc actttgtaaa cgcgtcatca tattaataat ttgttgtggt
    12241 gttaaagtga ttttagacat agatgtttta gcaacagcag catcattttg aaaataaaac
    12301 tcacgactct ttccacaaca agatgcaatc attcgttttg tggaatattg aaagcgatcg
    12361 cctaaatctt tattcagttc aacatgggca aatcctttac tatcatcaat cagtacagat
    12421 tttaattcat ctcgctttaa aatggcacct tccgaagcca gaaatccaat gactaactcc
    12481 tcaaggtttg ttggactgca tataacagtc gcaaattctt caccattcac cataattgta
    12541 agtggaaatt ctgtcacata ttgatctgtt gtattgaata attttccatc ttcatatcta
    12601 acaattggtt gacctaaaga tacatctttg ttcattatct aaccccttta attagcttga
    12661 actttatttt aaatcaatat gcttaatatt ttaacatatt tgcttaaaac tgaaatttga
    12721 ttaactaaat aaaatagcag gcaattattt agaatttgat tggatttaga aaaaggcaca
    12781 ctagcagctc aattaataaa aaataatttt tagaaagtgt gacaattatt tgaatcatta
    12841 tttaaagaga aaacgcttct tttca
//
LOCUS       DATTQF010000070        12813 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.070, whole genome shotgun sequence.
ACCESSION   DATTQF010000070 DATTQF010000000
VERSION     DATTQF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.070"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            237..2333
                     /gene="pnp"
                     /locus_tag="VB241_001622"
     CDS             237..2333
                     /gene="pnp"
                     /locus_tag="VB241_001622"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP1244914.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPTERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2569..4242
                     /locus_tag="VB241_001623"
     CDS             2569..4242
                     /locus_tag="VB241_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP1244915.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKEKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4499..6868
                     /locus_tag="VB241_001624"
     CDS             4499..6868
                     /locus_tag="VB241_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP1244916.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLIEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6873..7586
                     /locus_tag="VB241_001625"
     CDS             6873..7586
                     /locus_tag="VB241_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1244917.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7617..8882
                     /locus_tag="VB241_001626"
     CDS             7617..8882
                     /locus_tag="VB241_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP1244918.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8882..10165
                     /locus_tag="VB241_001627"
     CDS             8882..10165
                     /locus_tag="VB241_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP1244919.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEDLFTAFAEENAQANAFTSFDRTSY
                     LFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLRA
                     MYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVKQ
                     HEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYVQ
                     RDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDKL
                     KKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVFD
                     MLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10165..10869
                     /locus_tag="VB241_001628"
     CDS             10165..10869
                     /locus_tag="VB241_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP1244920.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTNKFN
                     DDKVHFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10974..11801
                     /locus_tag="VB241_001629"
     CDS             10974..11801
                     /locus_tag="VB241_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HEP1244921.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11820..12212
                     /locus_tag="VB241_001630"
     CDS             11820..12212
                     /locus_tag="VB241_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1244922.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12246..>12813
                     /gene="pgsA"
                     /locus_tag="VB241_001631"
     CDS             12246..>12813
                     /gene="pgsA"
                     /locus_tag="VB241_001631"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP1244923.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVF"
BASE COUNT         4676 a         1714 c         2471 g         3952 t
ORIGIN      
        1 ataaagatga tataagtaca cctagagtaa taatcaagat attaaaaata aagtatgttt
       61 tttaaaaaaa tataacttat atttatactg ataagggtgg gacgataagt ctattttgta
      121 aataatagat ggatatcccg ctctcttttt ttccaattca atattttata actaatatta
      181 aaatacgata ataaatgata tgatataact attagattca agagaggaga tttataatgt
      241 ctcaagaaaa gaaagttttt aaaactgaat gggcaggaag atctttaacg attgaaacag
      301 ggcaattagc taaacaagca aatggcgctg tattggttcg ttatggagat acagtcgtgt
      361 tatcgacggc aactgcatca aaagaacctc gtgatggaga tttcttccca ttaacagtga
      421 actatgaaga aaaaatgtac gctgcgggta aaattcctgg tggatttaaa aagagagaag
      481 gacgtcctgg tgacgatgca acattaactg cgcgattaat tgatagacca attagacctt
      541 tattccctaa aggatataag catgatgttc aaattatgaa catggtatta agtgcagatc
      601 ctgattgttc accacaaatg gctgcaatga ttggttcatc tatggcgctt agtgtgtcgg
      661 atattccatt ccaagggcca atcgccggtg taaatgtggg ttatattgat ggtaaatata
      721 tcattaaccc aacagtagaa gaaaaagaag tttctcgttt agaccttgaa gtagctggtc
      781 ataaagatgc agtaaacatg gtagaagcag gcgctagtga gattactgaa caagaaatgt
      841 tagaggcgat tttctttggt catgaagaga ttcaacgttt agttgatttc caacaacaaa
      901 tcgtcgacca cattcaacct gttaaacaag aatttattcc aacagagcgt gatgaagcgc
      961 tagttgaacg tgtaaaatct ttaaccgaag aaaaaggact taaagaaaca gttttaacat
     1021 ttgataaaca acaacgagat gaaaatcttg ataacttaaa agaagaaatc gtcaatgaat
     1081 ttatcgatga agaagatcca gagaatgaat tacttattaa agaagtttat gcaattttaa
     1141 atgaattagt gaaagaagaa gttcgacgtt taattgcaga tgaaaaaatt agaccagacg
     1201 gccgtaaacc tgatgaaatc cgtccattag attctgaagt tggtatttta cctagaacgc
     1261 atggttcagg tctatttaca cgtggtcaaa ctcaagcact ttcagtttta acattaggtg
     1321 ctttaggtga ttatcaatta attgatggtt taggacctga agaagaaaaa agattcatgc
     1381 atcattataa cttcccgaat ttttcagtag gtgaaactgg tccagtacgt gcgccaggtc
     1441 gtcgtgaaat tggacatggt gcgttaggtg aaagagcatt aaaatatatt attcctgata
     1501 ctgctgattt cccatataca attcgtattg taagtgaggt acttgaatca aacggttcat
     1561 catctcaagc gtcaatttgt ggatcaacat tagctttaat ggatgcgggc gtaccgatta
     1621 aagcaccagt tgctggtatt gctatgggcc ttgttacacg tgaagatagc tatacgattt
     1681 taacagatat ccaaggtatg gaagatgcat taggtgatat ggactttaaa gtcgctggta
     1741 ctaaagaagg tattacagca atccaaatgg atattaaaat tgacggttta acgcgtgaaa
     1801 ttatcgaaga ggctctagaa caagcgagac gtggtcgttt agaaataatg aatcatatgt
     1861 tacaaacaat tgatcaacca cgtactgaat taagtgctta cgcgccaaaa gttgtaacta
     1921 tgacaattaa accagataag attagagatg ttatcggacc tggtggtaaa aaaattaacg
     1981 aaattattga tgaaacaggc gttaaattag atatcgaaca agatggtact atctttattg
     2041 gtgccgttga tcaagctatg ataaatcgtg ctcgtgaaat cattgaggaa attacacgtg
     2101 aagcggaagt aggtcaaact tatcaagcca ctgttaaacg tattgaaaaa tacggtgcgt
     2161 ttgtaggcct attcccgggt aaagatgcgt tgcttcacat ttcacaaatt tcaaaaaata
     2221 gaattgaaaa agtggaagat gtattaaaaa ttggtgacac aattgaagtt aaaattactg
     2281 aaattgataa acaaggtcga gtaaatgcat cacacagagc attagaagaa taatatttaa
     2341 agtcatatga cgacaatgta tcgtcatgtg atttttttat gccatttttt acgaagtgac
     2401 ccattttgaa tttgttgtat tgaacatttt aaaacgcttt attattttgt gtacaactgt
     2461 taattatcct gtatgtatag tgattaatag tgtacatcaa gtatttttta acttataatg
     2521 aatagtgagt ttatatatgg acgggtaaca aatttaggag gtaagatttt gagtttaata
     2581 aagaaaaaga ataaagatat tcgcattata ccattaggcg gtgttggcga aattgctaaa
     2641 aatatgtata tcgttgaagt agacgatgaa atgtttatgt tagatgctgg acttatgttt
     2701 ccagaagacg aaatgctagg tattgatatt gttataccag acatttcata cgtacttgaa
     2761 aataaagaaa aattgaaggg tatattcctt acacacggac atgagcacgc gattggtgca
     2821 gtgagttatg ttttagaaca attagatgca ccagtatatg gatctaaatt gacaatagcg
     2881 ttaattaaag aaaatatgaa agcccgtaat attgataaaa aagttcgcta ctacacagtt
     2941 aataatgatt caattatgag attcaaaaac gtgaatatta gtttctttaa tacgacacac
     3001 agtattcctg atagtttagg tgtttgtatt cacacttcat atggtgccat tgtgtataca
     3061 ggtgaattta agtttgacca aagtttacat ggacattatg caccagatat taaacgtatg
     3121 gcagagattg gtgaagaagg cgtatttgtc ttaatcagtg attctactga ggcagagaaa
     3181 cctggatata atactccgga aaatgtgatt gaacatcata tgtatgatgc ttttgcaaaa
     3241 gtgcgaggtc gcttgattgt ttcatgttat gcttcgaact ttatacgtat tcagcaagtt
     3301 ttaaatattg ctagcaagct aaatcgtaaa gtgtcatttt taggaagatc acttgaaagt
     3361 tcatttaata ttgctcgtaa aatggggtat ttcgacattc ctaaagattt gctaattcct
     3421 ataacagaag ttgataatta tcctaaaaat gaagtgataa ttatagctac tggtatgcaa
     3481 ggagaacctg tagaagcctt aagtcaaatg gcgcaacata agcataaaat tatgaatatc
     3541 gaagaaggcg attctgtatt tttagcaatt acggcttctg ctaatatgga agttatcatt
     3601 gcgaatacat taaatgagct tgtacgtgct ggcgcacata ttattccaaa taacaagaag
     3661 attcatgctt caagtcatgg ttgcatggaa gaattaaaaa tgatgattaa tattatgaaa
     3721 cctgaatact ttattcctgt acaaggtgaa tttaaaatgc agatagcaca tgcgaagcta
     3781 gcagctgaag caggtgttgc accagaaaag attttccttg tggaaaaagg agatgtcatt
     3841 aattacaacg gtaaagatat gatattaaat gaaaaggtaa attcaggaaa tattttaata
     3901 gatggtattg gtattgggga tgtaggaaat atcgtgttga gagaccgtca tcttttagca
     3961 gaagatggta tctttattgc tgtagtaacg ttagatccta aaaatagacg tatagctgcg
     4021 ggacctgaaa ttcaatctcg tgggtttgta tatgtacgtg aaagtgaaga cttattacgt
     4081 gaagcagaag agaaagtacg tgaaatagta gaagctggtt tacaagaaaa acgcatagaa
     4141 tggtctgaaa ttaagcaaaa tatgcgtgat caaattagta aactattatt cgaaagtaca
     4201 aaacgtcgtc ctatgattat tccagtaatt tctgaaattt aatcaaaaag tcattaacat
     4261 aaaagaggtc agaacaagtc accgaaatat aatggttgtc atggacaatt tacttatatt
     4321 ttatgatggt caattgaaag gggtaacgat taatctgtta tcttaagtaa attgatacat
     4381 agatgatatt gttctaacct ctttcatcgt ctgtttggac tacatattct aaacatcaaa
     4441 taggaaatta tatagattaa cgtcgtttta actaaagcaa cataaggagg tgcgtcaatt
     4501 ggcacaagca aaaaagaaat cgacagctaa gaaaaaaaca acatcaaaaa aaagaacaaa
     4561 ttcgaggaaa aagaagaatg ataatccgat acgttatgtc atagctattt tagtagttgt
     4621 attaatggtg ttgggtgttt tccaattagg aataataggt cgtctaattg acagcttctt
     4681 taattattta tttgggtaca gtagatattt aacatatatt ttagtactct tagcaactgg
     4741 ttttattaca tactctaaac gtattcctaa aactagacga acggctggtt cgattgtatt
     4801 gcaaattgca ttgctatttg tatcacagtt agtttttcat tttaatagtg gtatcaaagc
     4861 tgaaagagaa cctgtacttt cttatgtgta tcagtcatac caacacagtc attttccaaa
     4921 ttttggtggc ggtgtattag gcttttattt attagagtta agcgtacctt taatttcatt
     4981 atttggtgta tgtattatta ctattttatt attatgctca agtgttattt tattaacaaa
     5041 ccatcaacat cgtgatgttg caaaagttgc actggaaaat ataaaagctt ggtttggttc
     5101 atttaatgaa aaaatgtcgg aaagaaacca agaaaaacaa ttgaagcgtg aagaaaaagc
     5161 gagacttaaa gaagaacaaa aggcacgtca aaatgaacag ccacaaataa aagatgtgag
     5221 tgattttacg gaagtgcctc aagaaagaga tattccaatt tatgggcata ctgaaaatga
     5281 aagtaaaagc cagagtcaac caagtcgaaa aaaacgagtg tttgatgcag agaatagttc
     5341 gaataacatc gtaaatcatc atcaagcaga tcagcaagaa caattaatag aacaaactca
     5401 taacagtgtt gaaagtgaaa acactattga agaagctggt gaagttacga atgtgtcgta
     5461 tgttgttcca ccgttaactt tacttaatca acctgcaaaa caaaaagcaa catctaaagc
     5521 tgaagtgcaa cgtaaaggac aagtactaga gaatacatta aaagattttg gggtaaatgc
     5581 aaaagtgaca caaattaaaa ttggtcctgc agtaactcaa tatgaaattc aaccagctca
     5641 aggggttaaa gtgagtaaaa ttgtaaactt gcataatgat attgcattag ctttagcagc
     5701 aaaagatgtt agaattgaag cgccaatacc tggtcgctct gcagtaggta ttgaagtgcc
     5761 aaatgagaaa atttcattag tttcactaaa agaagtttta gatgaaaaat tcccgtctaa
     5821 taataaacta gaagttggat taggaagaga tatatcaggt gatccaatta ctgttccact
     5881 aaatgaaatg ccacacttat tggtggcagg atcgacgggt agtggtaaat ctgtttgtat
     5941 aaatggtatt attacaagta ttttattaaa tgctaagccg catgaagtta aacttatgtt
     6001 aatcgatccg aaaatggttg aactaaatgt ttataacgga attccacatt tattaattcc
     6061 ggttgttaca aatcctcata aagctgctca agctttagaa aaaattgtag ctgagatgga
     6121 aagacgttat gatttattcc aacattcatc aactagaaat attaaaggtt ataacgaatt
     6181 aatccgtaag caaaatcaag aattagatga gaagcaacca gaattacctt atatcgttgt
     6241 tattgtagat gagcttgcag atttaatgat ggtagctggt aaagaagttg aaaatgcgat
     6301 tcaacgtatc acacaaatgg cacgtgcagc aggtatacat ttgattgtag cgacacaaag
     6361 accttctgtg gatgtaatta caggtatcat taaaaataac attccatcta gaattgcttt
     6421 tgctgtgagt tctcaaacag attcaagaac tattattggt actggcggcg cagaaaagtt
     6481 acttggtaaa ggtgacatgt tatacgttgg aaatggtgat tcatcacaaa cacgtattca
     6541 aggggcgttt ttaagtgacc aagaggtgca agatgttgta aattatgtag tagaacaaca
     6601 acaggcaaat tatgtaaaag aaatggaacc agatgcacca gtggataaat cggaaatgaa
     6661 aagtgaagat gctttatatg atgaagcgta tttgtttgtt gttgaacaac aaaaggcaag
     6721 tacatcattg ttacaacgcc aatttagaat tggttataat agagcatcta ggttgatgga
     6781 tgatttagaa cgcaatcagg taatcggtcc acaaaaagga agcaagccta gacaagtttt
     6841 aatagatctt aataatgacg aggtgtaaaa aaatgtcaga aatgaatgcg gtatataacg
     6901 ttaaacaata cattttaaat ttgattaagc aaaataaatt ggaatatggt gaccaacttc
     6961 caagtaattt atcaattgcc agagaattaa atgtaaaaac cgacgatgtt tatgaagcaa
     7021 ttcaggcatt gattactgaa caagtcatta aagataattt tgaagagggc acaagtgtta
     7081 agtcactgcc ccctttcttt tatccattga atgaacttat aagtattggg caaatgatta
     7141 aaaatgcagg atttgaatgc ggaactgaat acttaaattt tgatgagcaa ccagcaacta
     7201 tgttagatgc aaatttgttg agtgttgaag aaggatatcc agtaaccatt atagaacgat
     7261 tacgaactgc cgatggagaa ccggtcgtct attgtttaga taaaattgct aaaaaagaat
     7321 taacatgtac agagtatcaa atgagcaatg gatcgatact aagtgcgata aaagaacaaa
     7381 gtaatcataa tatttgttac gctgatacag aaattgaagc ggtaaattat gaacctcgaa
     7441 tatccgaagt actgaatgct tcgccacacg aaggtttgat tttattaaaa attacgcact
     7501 acaatgaatc agatgaacct attttgtatt cattaaatta tatgaaaaat agcttagttc
     7561 aatttaaaat cactagaaaa atatagatgt aagaagatat agaggaggct atatatttga
     7621 gtagacaatc tcaaccaaat atacatatca aagtttcacc aacaactaaa tttaaaacaa
     7681 ctactatagt ttttaaattt atggcacctt tagaatatga cacaataaca gctagatcat
     7741 tattaagcaa attattagtt cgagcaacta agaaatggcc aaccgataag tcatttaata
     7801 atcacttagc cgatttatat ggtgcgtatg tgaatagtac aatttcaaaa ttcaaagatc
     7861 agcatgtcat tacattttca ttagaaattg ttaatgaacg ttatttaaga aacggtgaat
     7921 cattatttaa tcaaggatta gatttattac aagaaatcat ttggaatcca ttaattgaaa
     7981 ataaagcatt caatgataat tttgttaacc aagagaaaac attattagcc aaaaaaatag
     8041 aagcaatggt agataacaaa gcacaatatt cgtttttaaa attactcgac catatgtttg
     8101 aaaatgaagc atataaatac ttatctacag gacaactaga acaaatccca catattactg
     8161 ctgaaacact atatcataca tatcaatcaa tgattaataa tgatcaatgt tctgtttatg
     8221 ttgtcggcaa tgtagaacct gaaagtgttg agaaacaaat acgtgaaaaa tttgcactta
     8281 aaccattcga taaacatcaa ttccaacatt ctactcatca tttacacgat gaagaagttg
     8341 attatattgt tgaatatgat gacgtggatc aagctaaatt aaatatggga taccgatttc
     8401 caacacaata tggacaaagt ggatatgctg cctttgttgt atttaacatg atgtttggag
     8461 gagatccttc atctgtttta tttaatgaag tgcgagaaaa gcaaagttta gcgtactcta
     8521 tacattcaca aattgatggc aaaaatggct atttatttgt tttgagtggg gtttcaagcg
     8581 ataagtacga aactgcaaaa gacactatta taagtgaatt tgaaaaaata aaagcaggag
     8641 atttcactga agaaaaatta gagttagcta aaaaagtaat catttctcat cgatatgaat
     8701 ctgaagatcg tccgaaaagt attatagaga ttatgcataa ccaaatatta ttagagcaac
     8761 cacaaagcaa agaaacattt ataaatgata tacagaaggt aagtcgcgaa gatattgttt
     8821 ctgttgctga aaaagcattt ttagatacaa tctatgtgtt gacaaaagga ggggataaat
     8881 aatgaaagag cgttattatg aattaataga cgaaagagta ttcgaacaag aattagaaaa
     8941 tggtttacga ttatttatta tccccaaacc aggttttcaa aagacatttg tcacttacac
     9001 tacacaattt ggttcattag ataatcaatt caaacccctt ggacaagacc aatttgttac
     9061 tgtacctgat ggagttgctc actttttaga acataaatta tttgaaaaag aagaagactt
     9121 atttactgcg tttgctgaag aaaatgcaca agcaaatgcg tttacaagct ttgatcgtac
     9181 aagctacttg ttcagtgcaa ctgataatat tgaaaacaac attaaacgtt tacttacaat
     9241 ggttgaaacg ccttatttta caaaagaaac tgttgataaa gaaaaaggta ttattgcaga
     9301 agaaataaaa atgtatcaag aacaacctgg atataaatta atgtttaata cattgcgtgc
     9361 aatgtatcaa caacatccaa tacgtgttga tattgccggt agtgtagaaa gtatatacga
     9421 tattacaaaa gatgatttgt atctatgtta tgaaacgttt tatcatccat caaatatggt
     9481 tttatttgtt gttggtgatg tggatcctga agcaatatgt cgaatagtaa aacaacacga
     9541 ggatgctcgt aataaagtta accaacccaa aatcgaacga ggacttgttg atgaaccgga
     9601 ggatgttaaa gaagcatttg ttactgaatc tatgaaaatt caatcaccaa gactaatgct
     9661 tggttttaaa aataaaccat tacaagaagc gcctcaaaaa tatgtacaac gtgatttaga
     9721 aatgtcatta ttctttgagt taatttttgg ggaagaaaca gatttttatc agaatttatt
     9781 aaacgaagga cttatcgatg atacatttgg ttatcaattt gtactagagc cgacgtatag
     9841 tttttcaatc gtgacaagcg ctactgaaga accagataaa ttaaaaaaat tattattaga
     9901 tgagttgcgc gataaaaaag gcaatttcca agatgcagaa gcatttgaac ttttgaaaaa
     9961 gcaatttata ggtgaattca tatcaagttt aaactcacct gaatatattg ctaatcaata
    10021 tactaaattg tattttgaag gtgttagtgt gtttgacatg ttagacattg ttgaaaacat
    10081 cacgttagat agtattaatg aaacgtcatc gttatattta aatttagatc agcaagtcga
    10141 tagtcgtttg gagattaaaa agtaatgaaa gcattagtat taggtggttc tggttcaatt
    10201 ggttctgaga tagtcaaaca attattaact gatggatttg aagtttatgt gcaatattat
    10261 cgtactgata taaatgaatt aactaacaaa tttaatgatg ataaagttca ttttatacaa
    10321 gcggatttat ctcaaacaat tgatattgac aaaacatttg gtgacattaa atcattagac
    10381 tgtttaatat atgcaagtgg tcagtcttta tatggtgttt tacaagatat gaaagaccat
    10441 gatattgatg catgttatca gttaaatgtc ttgcaattaa ttcgattatg tagatatttc
    10501 gttgatgttt tacgtcaaag tgacaatgga agaattattg taatttcatc aatttggggt
    10561 gagacaggag ctagcatgga aactatttat tcgacgatga aaagtgcaca attaggtttc
    10621 gttaaggcgc ttagtcaaga gcttgcacta acatcagtga cagtaaatgc tatcgcacct
    10681 ggttttgtag ctggtaatat ggcaagtgag tggcaagaag atgaacttca agcaatgata
    10741 actgaattac cacaacagcg attggtttta ccgagtgagg ttgctcatac atgcgcctat
    10801 ttatatcacc caaatgctag aagtgttact ggaactatac agaaagttaa tggggcttgg
    10861 tatatttaaa atatagaact atttttatga gcaaatgttt ttatgattga tgttataata
    10921 tgaatattac aatgaatcat aatttgttaa ttaaacaagg ggtgaggttc accatgacag
    10981 ttgcagagaa aaaagaatgg tacctagaat acgaaattga aattaataga ccgggtcttt
    11041 taggtgatgt atctagttta ttaggtatgt taggtataag tattgttaca attaatggtg
    11101 ttgatcaagg taaacgaggc cttttaatta aaacagacaa tcttgaaaaa gttgaacgtt
    11161 ttgagcaaat agctcgtggt ataaatgaaa ttgaaataac aaagcttaaa aaaccagaat
    11221 taagagaccg tcttgcagtt agacatggta gatatattga gcaagatgca aaagataaga
    11281 aaacctttcg atttgagcgt gaagatttag gcttgttagt agacttttta gctgaattgt
    11341 tcaaagaaga aggtcataag ttgattggca ttagaggtat gccacgggtt ggtaaaaccg
    11401 aatcaattgt tgcgggaagt gtttgtgcac ataagagatg gttatttatt agttctactt
    11461 taataaaaca aactgtacgt agctctctaa ttaaagggga atatgatgcc aatcatgtat
    11521 atattattga tggtgcagtt actgccagag aatctaatcc aaaacatcaa gagcttgtta
    11581 acgaagttat gacgttacct tcaatcaaag tcgttgaaca cccagattta tttgttgaaa
    11641 caagtacttg tacaatggaa gattttgact atattatcga attgagagaa aatgaaaatc
    11701 aagaaataca ttacgaagaa atgaagaaac agacagtcca aagtaagaat aatttagatt
    11761 ttggagatcc atttggtggt ggttttggtt tcttcgagta agtgtaagga ggctatgaat
    11821 tgaaaacggt cggtgaagcg ctaaaaggta gacgtgaaag gttaggaatg actttaacag
    11881 aattagagca acgtactgga attaaacgtg aaatgctagt gcatattgaa aataatgaat
    11941 tcgatcaact accgaataaa aattacagcg aaggatttat tagaaaatat gcaagcgtag
    12001 taaatattga acctaaccaa ttaattcaag ctcatcaaga tgaaattcca tcgaaccaag
    12061 ccgaatggga cgaagtaatt acagttttca ataataataa agacttagat tataagagta
    12121 aatcaaaaga gccaatacaa ttattagtaa tcatgggtat tacagtttta ataactttat
    12181 tgttatggat catgttagtt ttaatatttt aacagaaata aattagtgag aaatgaggat
    12241 gtataatgaa tattccgaac cagattacgg tttttagagt agtgttaata ccagttttta
    12301 tattgtttgc gttagttgat tttggatttg gcaatgtgtc atttctagga ggatatgaaa
    12361 taagaattga gttattaatc agtggtttta tttttatatt ggcttccctt agcgattttg
    12421 ttgatggtta tttagctaga aaatggaatt tagttacaaa tatggggaaa tttttggatc
    12481 cattagcgga taaattatta gttgcaagtg ctttaattgt tcttgtgcaa ctaggactaa
    12541 ccaattctgt agtagcaatc attattattg ccagagaatt tgccgtaact ggtttacgtt
    12601 tactacaaat tgaacaagga tttgtaagtg cagctggtca attaggtaaa attaaaacag
    12661 cagttactat ggtagcaatt acttggttgt tattaggtga tccattggca acattgattg
    12721 gtttgtcatt aggacaaatt ttattataca ttggcgttat ttttactatc ttatctggta
    12781 ttgaatactt ttataaaggt agagatgttt tta
//
LOCUS       DATTQF010000071        12707 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.071, whole genome shotgun sequence.
ACCESSION   DATTQF010000071 DATTQF010000000
VERSION     DATTQF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.071"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..173)
                     /locus_tag="VB241_001632"
     CDS             complement(<1..173)
                     /locus_tag="VB241_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEP1244924.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKV"
     gene            225..398
                     /locus_tag="VB241_001633"
     CDS             225..398
                     /locus_tag="VB241_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244925.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            complement(591..1520)
                     /locus_tag="VB241_001634"
     CDS             complement(591..1520)
                     /locus_tag="VB241_001634"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP1244926.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1573..2133)
                     /locus_tag="VB241_001635"
     CDS             complement(1573..2133)
                     /locus_tag="VB241_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP1244927.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            2506..3603
                     /locus_tag="VB241_001636"
     CDS             2506..3603
                     /locus_tag="VB241_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEP1244928.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNHGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKNCSFEGFVDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENINIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            3808..5370
                     /locus_tag="VB241_001637"
     CDS             3808..5370
                     /locus_tag="VB241_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEP1244929.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSTGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYIMPIV
                     LGIDINQPLPLK"
     gene            complement(5381..5488)
                     /locus_tag="VB241_001638"
     CDS             complement(5381..5488)
                     /locus_tag="VB241_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244930.1"
                     /translation="MVDFLLFVLFLHDATVYVSLPLVQAIANVTFYFSF"
     gene            complement(5560..6354)
                     /locus_tag="VB241_001639"
     CDS             complement(5560..6354)
                     /locus_tag="VB241_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEP1244931.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKSVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGSNTISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(6374..7450)
                     /locus_tag="VB241_001640"
     CDS             complement(6374..7450)
                     /locus_tag="VB241_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HEP1244932.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSINYHIAQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKYYEYPTSLIKEVPIEHDHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKKSNANQCPFHH"
     gene            7634..9103
                     /locus_tag="VB241_001641"
     CDS             7634..9103
                     /locus_tag="VB241_001641"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP1244933.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVYTYKMKFIKSFEAIDLHHNIYENGKLVYQIPTEDESRDYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            9096..9917
                     /gene="nadE"
                     /locus_tag="VB241_001642"
     CDS             9096..9917
                     /gene="nadE"
                     /locus_tag="VB241_001642"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP1244934.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIAELKQFIKSYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALQFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKQLYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            10187..10789
                     /locus_tag="VB241_001643"
     CDS             10187..10789
                     /locus_tag="VB241_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEP1244935.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            10770..10943
                     /locus_tag="VB241_001644"
     CDS             10770..10943
                     /locus_tag="VB241_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HEP1244936.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(11237..11560)
                     /locus_tag="VB241_001645"
     CDS             complement(11237..11560)
                     /locus_tag="VB241_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HEP1244937.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHK"
     gene            complement(11591..>12707)
                     /gene="scpA"
                     /locus_tag="VB241_001646"
     CDS             complement(11591..>12707)
                     /gene="scpA"
                     /locus_tag="VB241_001646"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HEP1244938.1"
                     /translation="SITPIANAESNSNIKAKDKRHVQVNVEDKSVPTDVRNLAQKDYL
                     SYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKVT
                     KDSSSSSKYTINVSSFLSKALNEYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLED
                     KKAKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFKIRETQGNN
                     GWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQQFQFTGLTPREMIYFGQTQGR
                     SPQLLNRMTTYNEVDNLTKNNKGIAILGSRVESRNGMHAGHAMAVVGNAKLNNGQEVI
                     IIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
BASE COUNT         4323 a         1869 c         2219 g         4296 t
ORIGIN      
        1 tcgactttat tttttcctaa atctgtatat agtgcttcta aaatatcgct ctcgtatgat
       61 ttaatagctt tgcttaactt ctttaattgc tcttttctaa aactaatatc tttagtttgt
      121 tgtgtattga aaaaagcttt actgtcataa aatttttgct caatgatatt cataatgaaa
      181 agaacctcct tatatgatta ttttggaaaa agtgattaat tgatttgaat gttgtggccg
      241 ttaattttaa atggtctttc gaattatata tgttgaaagt tgaaaataga gcgatgaatc
      301 gtgtacataa taatatttat aactttaatc ataacgaaaa aggtaggaag aaaacaaaaa
      361 tttatactca acatcgcaaa tatttcaaga aaatgtaaag gcaaaagggg aattgtatag
      421 aaatcactaa tctgtggatt aggatagcta aaggaatgaa aactgctatt gaaaaggttg
      481 taaattagtc aaacgtaaaa aaaaaacagt tcattgaaag tgaaataaat tctactttaa
      541 tgaactgtta gttaaataca acatgtctat aattagacag taatatagta ttattttgtt
      601 aatgcttcag tgatttgagg tacgatttgt ttttttcgag aaacaacacc agataagaag
      661 gccatgtcat cttctaattg aacattgaat gcttcgccaa ctttatcttt ttcagcacct
      721 acaactaaaa ttttagaatc actattaatg atgtcagtaa caacaagtac aaataagtca
      781 tatttttctt gtgcacttac agctaacatt tctttttcta aatcttcttt acgatttaac
      841 acttcgtcaa ggtcaacagc attaacttgt gcaatacgag tcacatagtc acccatagta
      901 aatgatttag catccatgtt taataagaat tcaactgatt tatcagttgt tgaagcacct
      961 gcttttaaca tatctaagcc gtacttttga atatcaactt tagcaatatc ttttaattct
     1021 tcagctgctt taacatcttg ttgtgtacat gttggtgatt tgaaaagtaa gctatctgaa
     1081 ataattgctg ataacattaa accagcaatt tcaggtttaa tttcaaagcc acgttctcta
     1141 aacattttgt ataaaattgt agctgtacaa ccaactggtt cagcacgata acataaagga
     1201 ccagcagttt cgaaatttgc aattctgtga tgatcaatta catgcttaat tgtagcagag
     1261 gcaatcgtat ctgaactttg ttggaattcg ttatgatcaa ctaagataac atcttggcca
     1321 tctaaatcat ccgttaataa ttccggagca ggtacattaa atgtatctaa cgcgaattga
     1381 gtttctgcac tcacatcacc taaacggtat gctttggctc ctgaattacc tctaagttgt
     1441 tcaaattctg ccataataat cgcagatgaa attgcatcag tgtctggatt cttatgtccg
     1501 aaaatatatg ttttagccat tgtcaaatat ctcccttgta tattgtattc tttataatta
     1561 ttttagcacg atttaaacgt gttgttctac ctctgcacct aatgagtttt tgaaatgtgc
     1621 aagtgcccat tcatgccctt tttgattaaa tgaagccact ccctcagcag gtactgaaat
     1681 tttatagcct aagttgtatg cagaaattgc tgtatgtaac acgcaaatgt cggtacatac
     1741 accaacgatt tcgacttgat taatacttct ttctctcaat aaactatcaa gcggggtacc
     1801 aaagaacgaa tcatagcgcg ttttatcaat gaaatgtaca ttaggttgcg ctttaattgt
     1861 ttcgtataat ttacctactt taccgtataa ttctctacca cttgtatcta cgatattgtg
     1921 tggtgggaat aatttacttt caggatgatg aatgtcatgt aaataatgta aatccattaa
     1981 aaagaatata tggtcttgat aataattaaa gtcattgata cgagaaacaa taaaatcttc
     2041 aatattttgt ccaggtttac cgcatgttag taagccgtcg tctgcgataa agtcatatga
     2101 atagtcaaca actaatagtg cgcgatgtgt cattcaacat ctctcctata taataataaa
     2161 ataaactccc atctattcat aaatgctaga gtatttttta accctaatgg ttaatatata
     2221 aatatagatt aagtatatag attaatcaac ttttttggaa gagcaaatca cgcaatcaac
     2281 aaatattata agatgttttt acgatagctt taaaatagct gtaatagaat actaaatgtg
     2341 acaaacttag aactaatatc aagtgttgat gttttcaata taaaaatgct aatagatggc
     2401 atcacgagat ttatgactat ctgctaaatc aacctaacac gattataatt tgaataattg
     2461 gttaatatat gaagtaatta gaaaatagac aaaggatgac gatttatgta tatcaatatg
     2521 aaagattatg ggttaacagg cataaacaaa actaaagata ctcgagcaat acaacgtgcg
     2581 ttaaatcatg gaagatgtaa accaacgaca gtttatatac cgaaagggac gtatgatatt
     2641 tgcaaaccat taacgatata tggcaataca acacttttgt tggataatga aactatttta
     2701 cgtcgatgtc attctggtcc gttattaaaa aatggtcatc gctttggttt ttatcgtggt
     2761 tataatggac acagtcatat tcatattaaa ggcggcaagt ttgatatgaa tggcgtatcg
     2821 tatccttata ataatacagc tatgtgcatt ggacatgctg aagatattca attaataggt
     2881 gtaaccatta agaatgtagt gagtggtcat gcaattgatg cttgtgggat taatggactc
     2941 tatattaaaa actgttcatt tgaaggattc gtagactata gtggcgaacg tttttattct
     3001 gaagcaatac aattagacat tcaagtacct ggtgcttttc caaaattcgg aacgacagat
     3061 ggtacgataa cgaaaaatgt cattatcgaa gattgttatt ttggaccttc agaattgccc
     3121 gaaatgggaa gttggaatcg tgctattggc tcacatgcaa gtagacataa tcgttactat
     3181 gagaatatta atattagaaa taatatattt gaagatatac aaggttatgc attaactccc
     3241 ttgaagtata aagatgcttt cattattaat aataagttta ttaactgtga gggtggtatt
     3301 agatatttag gagttagaga tggtaaaaat gctgcagatg tgatgacagg aaaagactta
     3361 ggttcccaag caggaataaa tatgaatata attggaaatg aatttaaagg gtcaatgtct
     3421 aaagatgcga tacatgtacg taattataat aatgttaaac ataaagatgt attaatcgtt
     3481 gggaatacat tcaataattc gactcaatca attcatttgg aagatattga tacagtgttt
     3541 ttaagtcctg ttgaagcggg tattcaagtt actacaatca atgtagatga aataaaaaag
     3601 taaaaagttt cgcatgacat taggattaag attactggat aatttttgaa agcgcattca
     3661 taaaacggta taaatatgct ataataaacc caattatctg ataaaagggg tattttgacg
     3721 gtaatgataa tacaagatag acaactttct atactctaat atagtgagtt gaagtagctt
     3781 gtcataatca tcatgagggg gaaatttatg gcttatttca atcaacatca atcaatgata
     3841 tcgaaaaggt atttaacatt cttttcaaaa tcaaagaaaa agaaaccgtt tagtacggga
     3901 caacttattg gactaatatt aggtccatta cttttcctat taacattatt gttctttcat
     3961 ccacaagact taccttggaa aggcgtctat gtcttagcga ttactttatg gattgcgact
     4021 tggtggatta ctgaagcaat tcctattgca gcaacgagct tattaccaat tgtgttatta
     4081 ccattaggtc acatacttac accagaacaa gtatcatccg aatatggcaa tgatattatc
     4141 tttttgtttt taggtggatt tattttggca attgcaatgg aaagatggaa tttacatacg
     4201 agagtagcgt tgacaattat taatttaatt ggtgcaagca cttcaaaaat attacttgga
     4261 ttcatggtgg caacaggatt cttatctatg tttgtatcga acactgcagc tgtaatgatc
     4321 atgattccga ttggtttagc aattattaag gaagcacatg atttacaaga agccaatacg
     4381 aatcaaacaa gtattcaaaa gtttgaaaaa tctctagttt tagcaattgg ctatgcaggt
     4441 acgattggcg gattaggtac acttatcgga acgccgccgc taattatttt aaaaggacaa
     4501 tacatgcaac attttggaca tgaaattagt tttgctaaat ggatgattgt agggattcca
     4561 acggtcattg tattgttagg tattacttgg ctctatttaa gatatgttgc gtttagacat
     4621 gatttgaaat atttacctgg tggtcagacg ttaattaaac aaaagttaga cgagcttggc
     4681 aaaatgaagt atgaagaaaa ggtagtacaa actatctttg tacttgccag cttattatgg
     4741 attacaagag agtttcttct gaaaaaatgg gaagttacgt catccgttgc agatggtacg
     4801 attgctattt ttatatcaat attattattt attattccag ctaaaaatac tgaaaaacat
     4861 cgccgtatca ttgactggga agttgcaaaa gagctccctt ggggtgtatt aattttattt
     4921 ggtggcggtt tagcattagc gaaaggtatt tctgaaagtg gtttagcaaa atggttaggc
     4981 gaacagttga aatcattaaa tggtgtcagc ccgattctta ttgtaatcgt cataactatc
     5041 tttgtcctat ttttaactga agtgacatct aataccgcaa ctgcaacgat gattttaccg
     5101 attttagcaa cgttatctgt tgctgttgga gtgcatccat tactacttat ggcacctgca
     5161 gctatggcgg ctaactgtgc atacatgtta ccagtaggga caccaccaaa tgcaattatc
     5221 tttggttcag gtaaaatatc tatcaaacaa atggcatcag taggattctg ggtaaactta
     5281 atcagtgcaa taattattat tttagtcgtg tattatataa tgcctatagt tttaggtatt
     5341 gatataaatc aaccactgcc attgaaatag taattgcaga ttagaacgaa aaataaaatg
     5401 ttacattagc aattgcttga acgagtggta acgaaacgta taccgtagca tcgtgtaaaa
     5461 acaatacaaa caaaagaaag tcaaccaagg atggattcct attttaatcc ttggttgact
     5521 ctttatttta tttaaattgt agaacctaga aaataaagtt taattaaaag caccaatcat
     5581 ttctactttg aaatctaagg tttctaaaat agcaatgact ttctttatat cggttgtaat
     5641 tgcagaatca gcctgaacga aaaatcgata catacctaat tgtgttttta aaggacgaga
     5701 ctcaatccag gataaattaa tattaaacaa agcaaatgta ttaagcacac ttgctaacaa
     5761 cccaggttta tcatgcattg gtgtaattaa aaacatcaat gatgtcgcat tttgatcaaa
     5821 ttgctgctga ttttttataa ctaaaaaacg tgtcacgtta tgtggatagt cttcaatatg
     5881 tgtatcaata ggtgtaaaac cataagcttc accactacct aaaggtgcaa ttgctgcaac
     5941 gccattttca attttagtca aactttgaat tgtactgtcg acataatcat agtcaaattg
     6001 atgttgatgt atgtaatttg ttgtttgact gattgctggt gcaatagaat acactttttt
     6061 aatatcagaa atggtgttcg aaccattacc atataatgca aagttaatat ctaaacgtat
     6121 ttcaccgtgt gcaaagacat cttgctgtgc aagtgcatct gccacaatgt tgattgttcc
     6181 ttctatagaa ttttcaatag ggacaacacc aatcgatgtg tcatcatctg caactgactt
     6241 gatgacttca aataaatttg actttggttg aaaagttgct tcattttcag aaaaatactg
     6301 acgacaagcc aaatatgaaa atgtaccttt agggcctaaa taatataatt gcatatgcta
     6361 tacctctact aacttaatga tggaaagggc actggttagc atttgatttt ttctttttat
     6421 agaaaaagtt tggatctttt actgtattgt catatccgtg atgataattt gacgtcaatg
     6481 ttggagataa tggcggtgct agccaagacc attttccggt aacttgacga ccttgttgtg
     6541 cttcattacg ttcgaatagt tcgaattgct ttgcagcggt caaatgatcg acaattgata
     6601 cgccttcttt tttaaaggaa tgatacacag catagttcaa ttcaacaagt gctcgatctt
     6661 tattaaatga attattttta agtgtatcaa attcaaacgc atctgcaact ttttctagta
     6721 aattgtaacg gtaatcatca ataaagttac gtacgccaat ttcagttacc atataccaac
     6781 cgttaaaggg ggcagttgga tacacaatgc caccgatttt taagtccata ttggaaatga
     6841 tagggactgc ataccatttt aagttcaatt ttcttaattt tggataatga tcatgttcaa
     6901 taggtacttc tttaattaat gacgtaggat attcgtaata tttaactgac tcattaggta
     6961 attggtaaat cagtggtaac acgtcaaaat tagtaccttt tcctttccaa cctaagtgat
     7021 ttgctaagcg tgtaacttct ttttcagcag gatcaccaca attgtcatag ccagcatagc
     7081 gaattaattg attgttgaaa attttaggtc catcctttgg agcatatata gtaatatacg
     7141 gctttaattt accttcatta gtagcctgtg caatatgata attaattgat gataagaacg
     7201 atgcttcgtc agtaacatct cttgcatcaa tgacatttaa cgaatcccaa aataaacgac
     7261 caatgcaacg atttgaatta cgccaagcca ttttagcacc ataaataagt tcttcttctg
     7321 tatgtgtata tgtcccagtt tcttttattt ctagttcaat gtcatgtaaa cgtttattga
     7381 taatttgcgt ttcataatga cactctttat acatgttttc tatgaaagct tgagcctctt
     7441 taaataacat taacaacacc tcgctttata ttatagtcta cattattaaa atactcttaa
     7501 aaattatgta tatgtcatta aattgttggt tgattttaat taaaagtatg gaaattaagg
     7561 ggctcttatg tatataaaaa aatgaattat gataaaatgt aagaaaatat ttaggtcgat
     7621 tggagagata cgagtgtacc aattagaaga cgacagttta atgttacata atgacttata
     7681 tcaaataaat atggctgaaa gttattggaa tgataatatt catgaaaaaa tggctgtatt
     7741 tgatttatat tttagaaaaa tgccatttaa tagtggctat gctgttttta atggtttaaa
     7801 acgcgtcatt gattttatag aacattttgg attttcagaa tctgatttgg aatatttaaa
     7861 gtctattggc tacaaggatg atttcttatc atatttaaaa gatttaaaat tcactggcag
     7921 catccgttcg atgcaagaag gcgaattatg ctttggtaac gaaccattgt tacgcgtaga
     7981 agcaccattg attcaagcgc aattaataga aacaatttta ttaaacattg taaatttcca
     8041 tacactaatt acaacaaagg ctagtagaat tcgtcaaatt gcatcaaatg ataaattaat
     8101 ggagtttggt acacgtcgtg cgcaagaaat tgatgcagca ttgtggggcg ctagagctgc
     8161 ttacatcggg ggctttgatt ctacaagtaa tgttagggcg ggtaaattat ttggtatacc
     8221 tgtgtctggt acacatgcac acgcatttgt ccaaacttat ggagatgaat atgttgcctt
     8281 caaaaaatat gctgaaagac ataaaaattg tgtgttccta gtagatacat tccatacttt
     8341 aaaatctggc gtgccaaatg caataaaagt tgcaaaagaa ttaggtgaca aaattaactt
     8401 tgtaggtatt cgattggatt ctggagatat cgcttattta tctaaagagg ccagacgtat
     8461 gcttgatgaa gcaggattta ctgaaactaa aattatcgcg tctaatgatt tggatgaaga
     8521 aacgattacg agtttgaaag cacaaggtgc aaaagtagat tcttggggcg ttggtacaaa
     8581 gctgattaca ggatacgatc aaccagcatt aggtgcagta tataaacttg tagctattga
     8641 aaatgaagat ggttcatata gtgatcgtat taaattatca aataacgctg aaaaggttac
     8701 tacgccaggt aagaaaaatg tatatcgcat tataaacaag aaaacaggta aggcagaagg
     8761 cgattatatt actttggaaa atgaaaatcc atacgatgaa caacctttaa aattattcca
     8821 tccagtgtat acttataaaa tgaaatttat aaaatctttc gaagccattg atttgcatca
     8881 taatatttat gaaaatggta aattagtgta tcaaattcca acagaagatg aatcacgtga
     8941 ttatttagca caaggattac aatccatttg ggatgaaaac aagcgtttct taaatccaca
     9001 agagtaccca gtcgatttaa gtaaagcatg ttgggataat aaacataaac gtattttcga
     9061 agtggcggaa catgttaagg agatggaaga agataatgag taaattacaa gacgttattg
     9121 tacaagaaat gaaagtgaaa aagcgtatcg acagtgcgga agaaattgct gagttaaaac
     9181 aatttataaa aagttatgtc caatcacatt catttataaa atctttagtg ttaggtattt
     9241 caggaggaca agattctaca ttagttggaa aattagtgca aatgtctgtt aacgaattac
     9301 gtgaagaagg cattgattgt acgtttattg cagttaaatt accttatgga gttcaaaaag
     9361 atgctgatga agttgagcaa gctttacaat tcattgagcc agatgaaata gtaacagtca
     9421 atattaagcc tgcagttgat caaagtgtgc aatcattgaa agaagctggg attgtcctaa
     9481 cagacttcca aaaaggtaat gaaaaggctc gcgaacgtat gaaagtacaa ttttcaattg
     9541 cttcaaaccg acaaggtatt gtagtaggaa cagatcattc agctgaaaat ataactggtt
     9601 tttatacgaa atatggtgat ggtgctgcag atattgcacc tatattcggt ttgaataagc
     9661 gccaaggtcg tcagttatta gcttatcttg gtgcaccaaa acaattgtat gaaaaaacac
     9721 caactgctga tttagaagat gacaaaccac agcttccaga tgaagatgca ttaggtgtaa
     9781 cttatgaggc gattgataat tatttagaag gtaagccagt tacgccagaa gaacaaaaag
     9841 taattgaaaa tcattatata cggaatgcac acaaacgtga acttgcatat acaagataca
     9901 cgtggccaaa atcctaattt aattttttct tctaacgtgt gccttaaatt aaatatgagt
     9961 tagaattaat aacgttaaac cacattcaac taggctactt cagtatataa attgataatg
    10021 tatgaaataa agtaagtatg ttcatttgag aataaatttt atttatgaca aattcgctat
    10081 ttatttatga gagttttcgt actatattat attaatatgc atttattaag gttagtttga
    10141 agcagtttga tatttaaagt gtaattgaaa gagagtgggg cgccttatgt cattcgtaac
    10201 agaaaatcca tggttaatgg tactaactat atttatcatt aacgtttgtt atgtaacgtt
    10261 tttaacgatg cgaacaattt taacgttgaa aggttatcgt tatattgctg catcagttag
    10321 ttttttagaa gtattagttt atatcgttgg tttaggtttg gttatgtcta atttagacca
    10381 tattcaaaat attattgcct acgcatttgg tttttcaata ggtatcattg ttggtatgaa
    10441 aatagaagaa aaactggcat taggttatac agttgtaaat gtaacttcag cagaatatga
    10501 gttagattta ccgaatgaac ttcgaaattt aggatatggc gttacgcact atgctgcatt
    10561 tggtagagat ggtagtcgta tggtgatgca aattttaaca ccaagaaaat atgaacgtaa
    10621 attgatggat acgataaaaa atttagatcc gaaagcattt atcattgcgt atgaacctcg
    10681 aaacatacat ggtggattct ggactaaagg cattcgtcgt agaaagctta aagactatga
    10741 accagaagaa ctggaaagtg tagtagaaca tgaaattcaa agtaaatgag aacgaaacaa
    10801 ttgcagattg tttgtcacga atgaaaatgc aagggtatat gccggtaaaa cgtattgaaa
    10861 aacccgtgtt tcaggagcaa aaagatggca cggttgaagt atcacatcaa gaaatcattt
    10921 ttgtaggtaa gaaaatccaa taacataatc caatttaaat aaagactatt tgaggaggaa
    10981 aggctattca aagtttgagt aattttactt tgaatagcct atgtgtttat acatgcaaga
    11041 tgctcgatcc atattgtatg agaaaccccc agcaagctat ataaagtata tgctgggggt
    11101 tcttaatatt ttaaaatcat tgttaaatta tatatatcgt cgctttttct aaaaaaatct
    11161 cattgcatga aattttttct tcctagagtc ctttaataag agtaatagtt tacttaaata
    11221 tatctagata gtcttattat ttatgcttaa tgaaagtcat tctaggagaa gttcctaaag
    11281 cttctgtgtt cataattgtt agtagtattt tattatcatt tggtataaat atttcaataa
    11341 caattgagct attattttta ttatataatg tgagttgttt gtgttctgta tttatatatt
    11401 tgcaaatgta atcagaatcg tctgaggtac tgagaataaa ttttaaaggg ctatcattta
    11461 aatcttgagg atgccattct ccctcaataa tattaagata atacttagcc tctgaattac
    11521 atttgaattt atcaatacta aataattcaa attgttccat aatattattt acctttctaa
    11581 aatacaaatt ttaataacca taaatagatg aataccattg ataatggtcg ccattggata
    11641 ctggaataac attgttttta gcatcttgag tcataaaacc attatcccat ggattccata
    11701 taattataac ctcttgtcca ttgtttaatt tagcgttccc aacaactgcc atagcatgcc
    11761 ctgcgtgcat accatttctt gattctactc tactacctaa aatagcaatt cctttattat
    11821 ttttagtaag attgtcaact tcattatatg tagtcattct attaagaagt tgtggacttc
    11881 ttccctgagt ttgtccaaaa taaatcatct ctcttggcgt taaaccagta aattggaatt
    11941 gttgtccttg taagtttggg tgtaaaaatc tcatcacagc ttctgcatga tatttgttag
    12001 tattataagt cgcatttagt aatgcagaca tcgtatagcc tgcacaccaa ccattgttac
    12061 cttgagtttc tcttatcttg aaattctcaa gtttatttac atattgctcg ttgtaagtat
    12121 aattattact tttaaattga ctagttggca tagtgacaga agctttttgc tttagttgcg
    12181 ttacattatt gccagtaggt atactctcgg tcttttttgc ctttttatct tctagacgtg
    12241 gtgtttttag tactagttta gctttatgat tttgagtaac cacatagtaa ccttttgagt
    12301 ttgtcaaaat agtaatttgt tgatctttat attcatttaa ggctttagat aaaaatgaag
    12361 aaacattgat tgtgtattta gaagatgaac tagaatcctt agtgactttt ggtgaaattg
    12421 taactatata atcgatatta ccttcagtgt tcaaaacagg aaaataataa ttaccatcac
    12481 tctttttgtt gaatttataa attttaaaag gttcaccaag tgtatacgag gctttctctt
    12541 tattatagat tttatcaagt gaagtaacat aagaaagata gtctttttgc gctaaattac
    12601 gtacgtcagt tggaacactc ttgtcctcga cattaacttg cacatgcctc ttatcttttg
    12661 ctttgatatt tgaattgctc tcagcgtttg caataggggt aatactt
//
LOCUS       DATTQF010000072        12693 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.072, whole genome shotgun sequence.
ACCESSION   DATTQF010000072 DATTQF010000000
VERSION     DATTQF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.072"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            149..1444
                     /gene="pbp4"
                     /locus_tag="VB241_001647"
     CDS             149..1444
                     /gene="pbp4"
                     /locus_tag="VB241_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HEP1244939.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            complement(1561..1959)
                     /gene="tagD"
                     /locus_tag="VB241_001648"
     CDS             complement(1561..1959)
                     /gene="tagD"
                     /locus_tag="VB241_001648"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HEP1244940.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(2022..3083)
                     /locus_tag="VB241_001649"
     CDS             complement(2022..3083)
                     /locus_tag="VB241_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP1244941.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMEIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDVTLANTYNHEILVKAMQKATDIHLVSQMIVGDNDIDTHATSNNE
                     DFKRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFNGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(3080..4183)
                     /locus_tag="VB241_001650"
     CDS             complement(3080..4183)
                     /locus_tag="VB241_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEP1244942.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSLYQKTVLTTPKQAPYLSELSDDVNVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSILMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(4282..5094)
                     /gene="tagG"
                     /locus_tag="VB241_001651"
     CDS             complement(4282..5094)
                     /gene="tagG"
                     /locus_tag="VB241_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HEP1244943.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEIMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            5441..6235
                     /gene="tagH"
                     /locus_tag="VB241_001652"
     CDS             5441..6235
                     /gene="tagH"
                     /locus_tag="VB241_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HEP1244944.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDNIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(6295..7059)
                     /locus_tag="VB241_001653"
     CDS             complement(6295..7059)
                     /locus_tag="VB241_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEP1244945.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVGEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIEL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESKVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            7295..8047
                     /locus_tag="VB241_001654"
     CDS             7295..8047
                     /locus_tag="VB241_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEP1244946.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(8127..8771)
                     /locus_tag="VB241_001655"
     CDS             complement(8127..8771)
                     /locus_tag="VB241_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP1244947.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            8893..9636
                     /locus_tag="VB241_001656"
     CDS             8893..9636
                     /locus_tag="VB241_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP1244948.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLHNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            9630..10466
                     /locus_tag="VB241_001657"
     CDS             9630..10466
                     /locus_tag="VB241_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP1244949.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            10463..11392
                     /locus_tag="VB241_001658"
     CDS             10463..11392
                     /locus_tag="VB241_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEP1244950.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            12494..>12693
                     /locus_tag="VB241_001659"
     CDS             12494..>12693
                     /locus_tag="VB241_001659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1244951.1"
                     /translation="MNIPSDEQIEYYMGIPIEKSFNLMSDFKLNDKELTELMTLFREN
                     YKETEAKHLKIYDGMQEQLKTL"
BASE COUNT         4313 a         2114 c         1917 g         4349 t
ORIGIN      
        1 tttgcattat ttttcaaata aaccgcaaga aacttaattt tcaacaattt aattaaggtg
       61 aagtgaatta gcaatgattt ccgtaagtag gaatgaccat tgcttttagg gaatatttaa
      121 gaattggaaa agggaagatt aacgctttat gaaaaattta atatctatta tcatcatttt
      181 atgtttaaca ttaagtatta tgacaccata tgcacaagct gctaacagtg acgtaacccc
      241 tgtacaagca gcaaatcaat atggttatgc aggtttgtcg gctgcatacg aaccgacgag
      301 tgctgttaat gttagtcaaa ctggacaatt actgtatcaa tacaatatcg atactaagtg
      361 gaatccagcg tctatgacta aattaatgac aatgtactta acattggaag ctgtaaataa
      421 ggggcagctt tcacttgatg acacagtcac aatgacgaac aaagaatata ttatgtctac
      481 actacctgag ttgagtaata cgaaactata tcctggacaa gtatggacaa tcgcagacct
      541 attacaaatt acagtatcta attctagtaa tgccgcggca ttaattttag ctaagaaggt
      601 atcaaaaaac accagcgatt tcgttgattt aatgaataac aaagctaaag ctatcggaat
      661 gaaaaataca catttcgtca atccaacggg tgctgaaaat tcaagattac gtacatttgc
      721 accaacaaaa tataaagacc aagaacgtac tgtaacgact gctagagact acgccatttt
      781 agatttacac gtgattaaag agacacctaa aatattagac tttacaaagc agttagcacc
      841 aacaacgcat gcagttacgt attacacatt caacttttca ttggaaggtg caaaaatgag
      901 tttgcctggt acagatggtt taaaaactgg atcaagtgat acagcaaatt acaaccatac
      961 gattactact aaacgaggta aatttagaat taatcaagtt atcatgggtg caggagacta
     1021 taaaaacctt ggtggcgaga agcaacgtaa tatgatgggg aatgcattaa tggaacgttc
     1081 atttgatcag tataaatatg taaaaatatt gtctaaaggt gagcaaagga taaatggtaa
     1141 gaaatattat gttgaaaatg atctttacga tgttttacca agtgatttta gtaaaaaaga
     1201 ttataaactt gtagtcgaag atggtaaagt acacgcggac tatccaagag aatttattaa
     1261 taaagattat ggacctccaa ctgtagaagt tcatcagcca attatccaaa aggcaaatac
     1321 tgttgctaaa agtatgtggg aagaacatcc attattcact atcattggtg gtgcatgcct
     1381 agtcgctgga ttagcactaa ttgttcatat gataatcaat cgtttattta gaaaaagaaa
     1441 ataaaacata ctaaaaacgg acaagttgca cattataaag ctgcgaaact tgtccgtttt
     1501 atatttattt tataataacc tacgttaatt tatcgtttag tatcgatagt tctatataat
     1561 ttatttagca tctttaccat ataattcttg tttgatttta gtcgtcgaaa tgccttctgt
     1621 acgttttaaa taaatgactt cacatttatc ctttaagaag tcgaattcac cttcccagtc
     1681 atgtcccata acaaaaacat ctacatcaaa tttttcgaca tcgtcttctt tttgtcccca
     1741 gcccttttct ggaatgacta aatcgacata gcgtattgat tcaagcatca tttttcgttg
     1801 ttcataatca taataagatt ttttatgttt aatttgatta aattcatcag ttgataatgc
     1861 tactattaaa taatcgccca tctctcttgc acgacgaagc aattcgatat gaccatagtg
     1921 aagtaagtca tatgtgccat atgttattac acgtttcatt aagttatcct cctcatattg
     1981 tcacatcata ggattgcagt ttatggtcat caatgtacac attatcgttt taacattgat
     2041 tttgttcgat gtgcaaactt tctcccttta agttgaatca agaatctttt atatgtcggc
     2101 ctattaatgc ccacttgaat taatgtttgt cgccacaatt cgtatatttc tctatcccaa
     2161 ttctcaccat taaattcaac tgtatttata atgtcaaaca tattttgtga tacataatca
     2221 gaatgctgca tagacataat atagtctttt acaagttgaa ttgtcgtgtg cgttactgct
     2281 gggtgttcat tcatataata ttttaatgaa ttattgaata aaatacgatc aaccatatca
     2341 ctatataata gcctttgttc aggtaatagt aacatttcca tgactcgttg tcttattttc
     2401 ataatttctg tgatatatct tttaaaatct tcattgttac ttgtagcatg tgtatctata
     2461 tcgttatctc cgacaatcat ctgtgaaact aaatgtatat ctgtagcttt ttgcatcgct
     2521 ttgacaagta tttcgtgatt gtaagtgtta gctaaagtta cgtcacactg taattcagca
     2581 aatttagcac tgaataactt tccgtcaaaa gacatcattg ataacaaatc tggttgttcc
     2641 gttaatgtaa cgattcgatt gtctttaata aactcatcag cacttttcaa tttccattca
     2701 ccatggtaat tgtaagctcg ctgccctatt accatatcaa tattatcatt atttttgaat
     2761 gctgcagtta gcctatctaa tttacctggc aatacgattg atgttggatt tatgaccata
     2821 aatgcgtcac tatcatgtgg atctgtcatt ttgatctcca tcaaaccttt attaatacac
     2881 gtagctatcg aagcattttg taattgtatt aatgaaatat gcttgagttc tctttgtaac
     2941 cgttcaatca taggaattgt ttgatcagta gaaccaccat caatacaaag gattctatag
     3001 tgttctttac tctcaataga gattaacaat tgtcgaattg ttgcctcatt attacatgta
     3061 ggtatgatta tcgtaagcct cattttgtca ccatcttatc tatatattct gtgagctgat
     3121 gtaaactttt atcagtatta tacttatgcc aatctttaaa taatggactt aatagatgtt
     3181 ctttttcttg tatcgtcatt attaaatcct cttcagtata cactttgtag ctatccggta
     3241 ttgctttgta aaattgattc aggcctctca ccttatcata tgttccttca tcatacacat
     3301 aaaatatagt tggaatatct aacaagctag cttctattgg cagcgaacta tagtcgctaa
     3361 taattatatc tgacattaac attaatatag acgtatcgat cgaagataca tcatcaatgt
     3421 ctgaatgttc aattgatgga tgtagtttat taatcagtgt atatcctggt aaacattttt
     3481 caaaataagc tttatcaata gccctattat ctgctttatc ttctctatat gttggtacat
     3541 ataatgccaa cttatttgta attccatatt tatcctttaa ctctgcctta accgttgctc
     3601 tatcagctgt gtaatattta ttaattctcg gaagcccaaa atacaacatt tgctcttctg
     3661 ttgcacctaa agactgttta aaacattgtg acatttgttc acaacccact aagtaaaaat
     3721 ctgtcgcttg ataaacttta cggtactgct gaaccattgc cttgtcagac acatcgactt
     3781 gatgatctgt taagccaaag ttttttaacg caccacttgc atgccacgtt tgaacaatgt
     3841 gttgattaga agtcttatta tatccaccta gtaataggta ataattatcg ataataatca
     3901 tctgcgcgct tttcaaagcc ttaatttgtt ttaccagtgt tcgattagtc atttctatca
     3961 cattaacatc gtcgctaagt tcagataaat aaggcgcttg ttttggtgtt gttaaaaccg
     4021 ttttctgata caacgaatta tttaatgctt tgatgatagg cttaatatct tctggaaaag
     4081 tcatcataaa tacgatatgc ggtttatcaa tcacttgagg cgtaatcatt ttagaaagta
     4141 ttcgaactac caaatgataa aatttcttta ttaaaacgtt cataatacac caacttaata
     4201 tgttatttac ttaaattata aacaaaaatg aaccccactt ccatttatta atggttagcg
     4261 gggtttcgtc atataaatat attacaagaa gtctgcaaat tgatctctat atttcatgtg
     4321 taagtacgca ccaattgcaa agaaaatggc aacaataccg aaattgtata acattaattt
     4381 ccaatgatcc atgaaatacc attcgtgata taaaattgct gcacggtatg attcagcaat
     4441 aaagtaaact ggattatatt tcattatttc atgaattaaa ccactgatac catggttctt
     4501 tggtagccac aaaattggtg aaaagtaaaa taatattctt aatattgctt gcattaacat
     4561 ttgtgtatct ctaactaaca caccgagtgt tgatgttaat aacgtcaccg aggcagttaa
     4621 gaaaaaacaa aacggtacat atatcaataa ttgaatgata tgtattgatg gataaatacc
     4681 cgtaaacata catgcaatta tcacaagtaa aagtaagcct aaatgtccat aaaatctact
     4741 tgtcacaata tatgtcggta ttatcgataa cgggaagttc attttcgata cttgattaaa
     4801 cttttgtgta attgctttag taccttctaa aataccttgg ttgatgaaga accacatact
     4861 gataccaacc aataaccaat aaacaaaagg tacaccatga attggtgcat tacttcttat
     4921 tcctaatcca aaaaccatcc agtaaaccat aatttgcata acagggttaa ttaattccca
     4981 tgccacacct aaatagttac tatgattgat aattttaact tgaaactgag ccagtctttg
     5041 aattaaataa aagttcttta catgttcttt aaaaactgtt cctattgctg acattccatt
     5101 aaaccacact ttcaaatgtt taactatttc tctacttaac taaatagtat tataataatt
     5161 gttgtaaata ctatcactat acatggatgc tatcaaaatt attgtctagt tctttaaaat
     5221 attagtttat tacaaataca ttatagtata caatcatgta agttgaaata agtttagttt
     5281 ttaaatatca ttgttatcat tgatgattaa cattttgtgt caaaacacca actctgataa
     5341 taacaaaatc ttctatacac tttacaacag gttttaaaat ttaacaactg ttgagtagta
     5401 tattataatc tagataaatg tgaataagga aggtctacaa atgaacgttt cggtaaacat
     5461 taaaaatgta acaaaagaat atcgtattta tcgtacaaat aaagaacgta tgaaagatgc
     5521 gctcattccc aaacataaaa acaaaacatt tttcgcttta gatgacatta gtttaaaagc
     5581 atatgaaggt gacgtcatag ggcttgttgg catcaatggt tccggcaaat caacgttgag
     5641 caatatcatt gggggttcat tgtcgcctac tgttggaaaa gtggatcgta atggtgaagt
     5701 cagcgttatc gcaattagtg ctggcttgag tggacaactt acagggattg aaaatatcga
     5761 atttaaaatg ttatgtatgg gctttaagcg taaagaaatt aaagcgatga cacctaagat
     5821 tattgaattt agtgaacttg gtgagtttat ctatcaacca gttaaaaagt attcaagtgg
     5881 tatgcgtgca aaacttggtt tttcaattaa tatcacagtt aatccagata tcttagtcat
     5941 tgacgaagct ttatctgtag gtgaccaaac ttttgcacaa aaatgtttag ataatattta
     6001 cgagtttaaa gagcaaaaca aaaccatctt tttcgttagt cataacttag gccaagtgag
     6061 acaattttgt actaagattg cttggattga aggcggaaag ttaaaagatt acggtgaact
     6121 tgatgatgta ttacctaaat atgaagcttt cctaaacgat tttaaaaaga aatccaaagc
     6181 cgaacaaaaa gaatttagaa acaaactcga tgagtcccgc ttcgttatta aataaaccga
     6241 aaaaaccgag aatctccatt taaggatttc ctcggtttta ttttgtcatc atgactattt
     6301 tgcctttttt atttttcttt ttgctttggc tattttatac ataaatatag gaatactctt
     6361 taatctacca atacgtttcc aatctattaa tgctctatat atccattcaa tgtttaattt
     6421 tctaaagata taaggcgctc tctttttagc cccagcaaat acttcaagag aaccacctac
     6481 gcccatcatc actttagatt caaattgatt ttcatgtgtc ataatccatt cttcttgttt
     6541 agggaatccc atacctacaa atatgtaatc aggtttaaac agttcaattc gtttcactac
     6601 tgtctcatct tctaaatcaa tataaccatg atgatgtgca aacgatatgt ttggatatct
     6661 ttgttgcaat gcatattgtg ccgcttctcc aacttcatta gttgccccta gcaaaaatac
     6721 tttttgatga tttacatgag caattttcaa acattcatcc atcaactcaa taccaggtat
     6781 acgatgcgct agaggttgct ttaaacgatg cgaagctttg actactcctg tcccatcagc
     6841 aacaatatag ctcgcttgat ttattaactc taaatacgct tgatgtgtcg tcgcgtaatt
     6901 cactatttca gggttggctg ttactataaa aagattattc gttgattgat ttgcaaaaaa
     6961 ggttttaata ttttcaacca tttgcaacat tgttgtatta tcaaaatcga cccctaaaat
     7021 gtcaactttg gctgtattgg atctttcttc aacagtcata acaggaatct cctatcttat
     7081 ttcattatta tgttagtgta atcaatatta acagtattta tattcatcct atttatatcg
     7141 aaatgatttt gctttatttt aacacatatt caccttttca aaaattaaaa ttagtttaac
     7201 taaggtaagt ctaaagtcta acgctattga tgccaatatc atttataata agtgataagc
     7261 aaactttaaa ttaatatctt atatgaggtt aacaatgaat tatttatcca acttttttaa
     7321 tacgacaatt caacttaata tatacattat cgttattgtt gcagcttgtt atattgctat
     7381 tcatcaatat agacataagc ctgttttgaa ctatttagat gtcattttaa actatatccc
     7441 tgttttaaca catgaatttg gtcatgtact gtttaataaa ctcgctggtg gccgtgcaaa
     7501 agatttagtc attgtaacaa gcccacgaga gcgacaacag acattacaac aaggattcgc
     7561 aattacacaa tctagacatc tagcaggcca atggcttaca acgattggtg gttattttat
     7621 gccacctatt atgttattga ttggtctagc gtcgagccat tatcagatac ctagtttctt
     7681 tatatttacc tatttattga tatttattta ttttttaatt ttaacatcta gaaaaggctc
     7741 acccattgtt gttataactt taatttctat catgttgtat tttatattaa aagatgaaaa
     7801 tatagtagaa attcaactac ttgtcacaat gagttatcaa tatattttag gtgtattatt
     7861 aggcgaagtt ctacaatcta gttggacgat tgctaaattg actttccagc gtccgagtcc
     7921 acagtgggat ggtagtgcac ttaaagaact cagtcatgta cctatcttca tttatagtac
     7981 aatctggatt atcttcaatc tttatgcagt gaatatttta ctcaaatata ctcacttgat
     8041 aaattaacta ataaaaaaga agcctaaagc aattatccat atgtctttat cagtacaatc
     8101 atggatatct ttatggcttc tttattttat ttttcagcaa acatgttcat tgcattttcg
     8161 taactgacaa taattacatt atcattacgt ttgatgataa ttactttatt catttcatct
     8221 ttcgatacaa tttccacttc attaccaata gagatatctt tactagacaa atatattagc
     8281 aaatcagtct tatctctcac acgtttgatt gtaacgatat caccaggttc ataattcaaa
     8341 atcgttgtta tatatttctc tttatattca ttatttctag gaatcacacc gccgtgcggg
     8401 caagtttctg ggaaatttaa caagctatcc agcctttcaa caaataaatc tgaaattcga
     8461 tgttctaaaa tttctgcttc ttgatgtact tcttcccaat tatatttcaa tatttctatt
     8521 aaaaataatt ctaatagtcg atgtctctta atgatatcaa gcgtatgcgt taaaccatcc
     8581 tctgttaatc taacaccttt gtacggtttt gtttcaacat agcctgcttt ttcgagacgt
     8641 cctaccattt cacttacaga tggaggctta atatttaaaa attgagataa gattttattt
     8701 gtcacaaaat ttttatcgcc attattcgta aggattgcct ttaaatagtc ctctttttct
     8761 tcagttaaca tactttcacc tcacatacat tgtctatatt atatcacgaa tttacttgac
     8821 atgataaata ttctcagttt attatacaat taattaggtt agcctaaact tttaattagg
     8881 aggtataaac gtttgttaga aacaaaagat ttaaatctgt ttttaggtaa taagcatgta
     8941 cttaaaaaca tttccttatc gataccagta cgcggcgaaa taattggtat catgggcccg
     9001 aatggtgctg gtaaatcttc ccttatcaag tctttaattg gtgaatttaa tgctaccggt
     9061 actaaattgt tacataacaa acctatacaa caacaactgc aacatattac atatattcca
     9121 caaaaagcac atattgattt agattttcct ataagtgtgg aacaagtgat tttatcaggt
     9181 tgctacaaag aaattggatg gtttagacga cctaataaat cagcaaggga taaactcaaa
     9241 cagttattaa gcgatttaga attagaatct ttacgtcatc gacaaatttc agaattaagt
     9301 ggtggacaat tacaacgtgt gctagtagcg agagcattga tgtccgaaag tgaagtttat
     9361 tttctagatg agccgtttgt cggaattgat tttagtagtg aaaaattaat catgacaaaa
     9421 atcgagaact taaaacaaca aggaaaactt attcttatca tccaccatga tttatcaaaa
     9481 gcaaagcaat actttgatcg cattattcta ttaaatcaaa cattacgata ctttggtgat
     9541 agtgaagagg ctatgagtgt cactcgctta aacgaaacat ttatgagtag cactgactgt
     9601 agtgacccta gtcaaaggag caatataaca tgttagagtt tgtcgaacat ttatttacat
     9661 atcaattctt gaatcgagca ttgataactt caattattgt agggatagtt tgtggtacag
     9721 ttggtagttt aattgtatta cgtggtcttt cattaatggg agatgcaatg agtcacgcag
     9781 tattacctgg tgttgcccta tcattcttat ttggtattcc aatgtttgta ggcgcactta
     9841 taactggtat gatcgcaagt atttttatcg gttatatcac atctagtagt aaaacgaaac
     9901 ctgacgccgc tattggaatt agttttaccg ctttcttagc ttcggggatt attattatta
     9961 gcttaataaa cactacgaca gatttgtacc atattctgtt tggtaattta ctggcaatca
    10021 caaatagtgc atttttaaca actatagtga ttggttcaat cgtccttatt ctaatcatta
    10081 ttttctatcg tccattaatg atttctacat tcgatccaac ttttagtaga atgagtggtc
    10141 taaatacgac gttattacat tactttgtga tgttgttact ctcattagta acagtagcaa
    10201 gtattcaaac ggtaggtatt attcttgtag ttgcattatt aattacacct gcatctacag
    10261 catttttaat tagtaaaaaa ctatactcaa tgatgattat agcaagttta ataagtgtca
    10321 taagttcaat tgtcggactt tattacagct atatttacaa cattcctagt ggtgcaacaa
    10381 tcgtactttg tacgtttgta atttatatca tcacattatt tttcacaaaa tttacgaata
    10441 gaaagaaacg aggaagttta acatgaaaaa attagtacct ttattattag ccttattact
    10501 tttagttgct gcatgtggta ctggtggtaa acaaagcagt gataagtcaa atggcaaact
    10561 aaaagtagta acgacgaatt caattttata tgatatggct aaaaatgttg gtggagacaa
    10621 cgtcgatatt catagtattg tacctgttgg tcaagatcct catgaatatg aagttaaacc
    10681 taaagatatt aaaaagttaa ctgacgctga cgttatttta tacaacggat taaatttaga
    10741 gactggtaac ggttggtttg aaaaagcctt agaacaggct ggtaaatcat taaaagataa
    10801 aaaagttatc gcagtatcaa aagatgttaa acctatctat ttaaacggtg aagaaggcaa
    10861 caaagataaa caagatccac acgcctggtt aagtttagat aacggtatta aatacgtaaa
    10921 aacaattcaa caaacattta tcgataacga caaaaaacat aaagcagatt atgaaaagca
    10981 aggtaacaaa tacattgctc aattggaaaa attaaataac gacagtaaag acaaatttaa
    11041 tgacattcca aaagaacaac gtgccatgat tacaagtgaa ggtgccttca agtacttctc
    11101 aaaacaatac ggtattacac caggttatat ttgggaaatt aacactgaaa aacaaggtac
    11161 accagaacaa atgagacaag ctattgagtt tgttaaaaag cacaaattaa aacacttatt
    11221 agtagaaaca agtgttgata agaaagcaat ggaaagttta tctgaagaaa cgaagaaaga
    11281 tatctttggt gaagtgtaca cagattcaat cggtaaagaa ggcactaaag gtgactctta
    11341 ctacaaaatg atgaaatcaa atattgaaac tgtacacgga agcatgaaat aacacgctgt
    11401 gttttaatga agtaagatga attgatgttg atgcaaccta aaatattggt atctccaata
    11461 ttttaggcca cacatcaaca taacaaagtc gaaggctaat agtcccatat cgtgcgttaa
    11521 atatatatta ccctcctatt aatatatata ccgttcccga tcgcacgata tggtggtatt
    11581 agaacttctc tttgagcgaa agagaaaagc tagaagttct tatgcagttt taattaaact
    11641 ataaacattt gtcactcttt aaatcaaaga gtaaagttaa aagctttatg tgtttttaat
    11701 taaactgtcg ttcccttcat ctcttttaac cacagagatg cgttagaagt tcttctaata
    11761 caatttatac aacgccattc cctacacact cttataaaag agattcacgc gcgtcaataa
    11821 attgtattac atactaacta aaaagctttt cttaatcgta ctaacgaagt tagaggttct
    11881 tatgcgattg gacaactgtt ctccctacta gacttttagc atagctggtt aaaagtacta
    11941 ttacagattg ttcaattgta tgcccctttc ctttgaacgg aatattaaaa gctatgtgca
    12001 gtaactttta ataggcatac cacttttgca tcaagctata aataatcgca tactttgata
    12061 tttaatctgg cctatcaaag taaacatcta tggtttcctc cccccataga tccttaagcc
    12121 acactcatcc gatgtcatat gagtgtggct attttttgtg aatgcactta tcttcatctt
    12181 taaatgactt cgattaatct atctcctcca atttttaaaa cttatttcaa ttaaatctca
    12241 aatgttaaca acaccttcaa atccctttaa ttaagtttac ataaaattaa tttgcaatat
    12301 ttaattaatt tatatataat ataaattaat cgatactatt ttgaggtgat aaaatgttgc
    12361 ttttgcaaag tgaaataaaa aattctaaga actcacatgc atagttagaa gctgaaatca
    12421 acacttctca agaagtaatt caatctcaag gttttccctg tgtttttggt gtacatggcc
    12481 acaaaaaaga ggtttgaata tacctagcga tgaacaaata gaatattata tgggtattcc
    12541 gattgaaaaa tcttttaatt taatgagtga ttttaagcta aatgacaaag aactcaccga
    12601 actaatgaca ttgtttagag aaaattataa agaaactgaa gcaaaacatt taaaaatata
    12661 tgacggaatg caagaacagc tcaaaacact tca
//
LOCUS       DATTQF010000073        12630 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.073, whole genome shotgun sequence.
ACCESSION   DATTQF010000073 DATTQF010000000
VERSION     DATTQF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.073"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..393
                     /locus_tag="VB241_001660"
     CDS             <1..393
                     /locus_tag="VB241_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HEP1244952.1"
                     /translation="LADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKN
                     HIMPMIEKQITDINQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVD
                     VDKLPKKGIDITHGDKAFEKKLEKLEEK"
     gene            547..867
                     /gene="ytxJ"
                     /locus_tag="VB241_001661"
     CDS             547..867
                     /gene="ytxJ"
                     /locus_tag="VB241_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HEP1244953.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            1292..2416
                     /locus_tag="VB241_001662"
     CDS             1292..2416
                     /locus_tag="VB241_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP1244954.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(2604..3830)
                     /gene="pepT"
                     /locus_tag="VB241_001663"
     CDS             complement(2604..3830)
                     /gene="pepT"
                     /locus_tag="VB241_001663"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP1244955.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDTAENN"
     gene            complement(3844..4338)
                     /locus_tag="VB241_001664"
     CDS             complement(3844..4338)
                     /locus_tag="VB241_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEP1244956.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRSYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(4356..5117)
                     /locus_tag="VB241_001665"
     CDS             complement(4356..5117)
                     /locus_tag="VB241_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HEP1244957.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(5313..6383)
                     /locus_tag="VB241_001666"
     CDS             complement(5313..6383)
                     /locus_tag="VB241_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEP1244958.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            6701..7756
                     /locus_tag="VB241_001667"
     CDS             6701..7756
                     /locus_tag="VB241_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP1244959.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            complement(7920..8561)
                     /locus_tag="VB241_001668"
     CDS             complement(7920..8561)
                     /locus_tag="VB241_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP1244960.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            8705..9571
                     /gene="fakB1"
                     /locus_tag="VB241_001669"
     CDS             8705..9571
                     /gene="fakB1"
                     /locus_tag="VB241_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HEP1244961.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            9935..11017
                     /locus_tag="VB241_001670"
     CDS             9935..11017
                     /locus_tag="VB241_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1244962.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPLENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSIYKQKITKLTYVHSEDVFRFEKVEQLRNGQY
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQKMNKLALKRGWIDE"
     gene            11010..11684
                     /locus_tag="VB241_001671"
     CDS             11010..11684
                     /locus_tag="VB241_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP1244963.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKYLDQNEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFSKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            11745..12317
                     /gene="raiA"
                     /locus_tag="VB241_001672"
     CDS             11745..12317
                     /gene="raiA"
                     /locus_tag="VB241_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP1244964.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
BASE COUNT         4459 a         1822 c         2189 g         4160 t
ORIGIN      
        1 ttagctgata ataaaagtga agcaactaat cttacgacaa aattagaaca taataataaa
       61 gcgttaagag atactgcgaa aaagaaccta gatgatagta aagaaaatga agtaaaaggc
      121 gcgattaaaa atcacattat gccaatgatt gaaaaacaaa ttaccgatat taaccaaact
      181 aatattagtg ataagcatgt taataatgca aggaaaaacg caatagaaat gtattacagt
      241 ctgcagaact attataatac acgtattgaa acaataaagg ttagtgagaa gttatcaaaa
      301 gtcgatgtag ataagttgcc gaaaaagggt atagatataa ctcacggcga taaagccttt
      361 gaaaaaaagc ttgaaaaatt agaagaaaaa taactataat catttttcaa agttaaaaat
      421 tttgaattta tggttaacat gtcaacttac tatgtgtata atggtaaaca ttgatattaa
      481 ctatatgtat aaaaatgtca cgcagatgct atttaaatgt gataaatatt tttagaggtg
      541 aatagagtgg ctataaagct aagttcaatt gaccaatttg aacaggttat tgaggaaaat
      601 aaatatgttt ttgtattaaa acatagtgaa acttgtccaa tatcggcaaa tgcgtacgat
      661 caatttaata aatttttata tgaacgcgat atggacggtt attatttgat tgtccaacaa
      721 gaacgcgatt tgtcagatta tattgctaaa aaaacgaacg ttaaacatga atcacctcaa
      781 gcattttatt ttgtaaatgg tgaaatggtt tggaatcgag accacggtga tattaatgtg
      841 tcgtcattag cacaagcaga agaataatga aactataggg ttggaacatt ttgccttaca
      901 ctactagacg tgaatagcac aacttaaatt cgtgtgaatc agagtagttt ggctataatg
      961 atgttctgac cttttatttt atgtaacctt tagaagcagt taagttagta cttttttaca
     1021 aacatatgta taatgtattc gagtattttt attgaaaata ttttggaaaa cgacgaatcc
     1081 aataagaaaa ttttaacatg atttgtaagt tagtttaata ggaaatacat gctaaaccat
     1141 aagaagtata ctgttatttg ctggaataat taataatcat gtcatgttaa atgttcgcat
     1201 ataatcacga gataaaatct aaaatttaag attaatcttt tatgaataaa aaacgtatca
     1261 caacaaataa taaagtaagg tggtcaaggt tatgaaagta ttagtagcca tggatgagtt
     1321 tcatggaatt atttcgagtt atcaagctaa tagatatgtt gaagaggcag ttgcaagcca
     1381 aattgaaact gcagatgtag ttcaagtacc attgtttaat ggaagacatg aattattaga
     1441 ttctgtattt ttatggcaat ctgggcaaaa gtatcgtata ccagtacatg atgcagatat
     1501 gaatgaagtt gaaggtgttt acggacaaac tgatacaggg atgaccgtta tcgaggggaa
     1561 tttattttta aaaggtaaaa aaccaattgt tgaacgaaca agttatggtt taggagaaat
     1621 gattaaacat gcattagata acgacgcaaa acatgttgta atttcattag gtgggattga
     1681 tagttttgat gctggtgcag gtatgttaca agcattaggt gctcaattct atgatgacga
     1741 agggcgtgtc gtagatatga gacaaggtgc tggtgtaatt aaatatattc gtcgtatgga
     1801 tatgtcgaac ttacacccta aaatggaaac agcaagaatt caagtaatgt cggatttttc
     1861 aagtcgatta tatggtaagc aaagtgaaat catgcaaact tatgatgcgc atcagttgaa
     1921 ccataatcaa gcagcagaaa tcgataattt aatttggtat tttagtgagt tatttaaaag
     1981 tgaattgaaa attgcaattg gtccagttga acgtggtggt gctggtggtg gaattgcagc
     2041 agtcttgaat ggactgtatc aagctgaaat attaaccagt catgcattag tagaccaact
     2101 aacacattta gaaaatttag ttgaacaagc ggatttaatt atttttggag aaggattaaa
     2161 tgaaaatgat cagttgctag aaacgacaac attgcgtatt gcagaacttt gtcataaaca
     2221 tcaaaaggtt gccattgcaa tttgtgcaac tgctgaaaag tttgatttat ttgaatcaca
     2281 aggggttaca gcaatgttta atacatttat cgatatgccg gaaagttata ccgactttaa
     2341 aatgggatta caaattaggc attatactgt tcagtcttta aaactgttga aaacacattt
     2401 taatgttgag gtttagtaaa gaaggactaa attggtgatg ctgtcatgat ggttaataac
     2461 atttatgatg gttagcaaaa cgaattagaa gatcgaaagt atacgtaaaa aatatgaaaa
     2521 atcacgctat cattgcactg aatgttagcg tgatttttta tattaattaa gcctgagttg
     2581 aactagtata taatcgtttg tttttagtta ttttcagcgg tatcttctac aattccaatg
     2641 attacttgta ctgctttttc catgacatca atggatgcat attcatatgg gccgtggaag
     2701 ttaccgcaac ctgtaaagat gtttggagtt ggtaatccca taaatgacaa ttgtgaacca
     2761 tctgtaccac cacgaatagg ttcagtgttt gctggaatat ctaatttagc aaagacacgt
     2821 ttaggtatat caataatatg aggcaatggt aatatttttt ctgccatatt gaaatattga
     2881 tccgatatat caactttaac tggataattt tcaaaatggg cattgatatc gtcacgtatt
     2941 tctaaaatac gtttcttacg caattcgaat tgttttttat catgatcacg aataatgtat
     3001 tgcaaagttg ctttttcaac agttccttca aagttcatta agtggtaaaa gccttcatat
     3061 ccttctgttc gctccggaac ttcactatca ggtagcaaac tatcgaattg ttcacctaaa
     3121 cgtattgcgt ttaccattgc atttttagct gaaccaggat gaacattcac accgtggcat
     3181 gtaataaccg cttcagcagc gttaaagctt tcatattgta attctccata ttgactacca
     3241 tccatagtat aagcaaaatc agcattgaag cggtcaacat caaatttatg tggaccacga
     3301 ccgatttctt cgtctggtgt aaatccaatg cgaatggtac catgtttaat ttctggatgt
     3361 tcttgtaaat aacaaatagc ttccataatt tccacaatac ccgctttatc gtctgcacct
     3421 agtaacgatg taccatcagt taccattaat gtatgaccaa ctaaactgtt aagttctgga
     3481 aatactttag gatctaagac acgtttagta ttgcctagtt tgtatggctt gccatcatag
     3541 ttttcaataa tttgcggttt aacatttgaa gcattgaaat caggtgatgt atcaacatga
     3601 gccaaaaaac caacagttgg gacgtcggca tcgatgttac tttctaatgt agcaaataag
     3661 tagccatttt catctaaatc agttggcaat cctaattgtt gtaattcttt ttctaataaa
     3721 tgtaacaaat cccattgctt ttcagttgaa ggtgttgttg tagattttgg atcagattgc
     3781 gtatcaattg tcgtatatct tgttaatcta tctatcaatt ggttcttcat tatattcgac
     3841 cccttaaact ctattattca tgttgtaaga ttttttatat gtcttacctt tgattttacc
     3901 atacagttgt ttgatacgtg tgtacaggta atatagaatt tcagaaacta atataccgaa
     3961 agcaatcgca cctgaaatca gtgtaacttc taaaaatgta tttacagcac ttgtataatc
     4021 attcgatact aaaaaacgag tcgcttgata agctgcacca ccaggtacta atggtataat
     4081 gcctggcact atgaatataa ttaccggtcg tttataactg cgactcatag tatgactcat
     4141 taagcctaaa attaagcttc ccaaaaatga agcgccaact tttccaaact ctaaatctac
     4201 cgttaattgg taaatcgtcc atgcaatggc acctacaaat ccacatgcta ctaagagacg
     4261 tttgggtgca ttgaaaatga tagagaaaag tactgttgat ataaagctga ttgtaaaatg
     4321 aaataaataa aatagcatgc tttaacagtc cttccttaaa tgattaataa aacgattgcg
     4381 acaccagcac cgattgcgaa tgctgttaat gcagcttcaa caccacgaga catacctgca
     4441 agtaattcac ccgctaataa atctcgaatg gcattggtaa ttaatatacc agggacaagt
     4501 ggcatgacac tggctatagt aatgatatct tgattggttg caatacctaa tttagtaaat
     4561 gtggctgcaa tggatatgac cacagcggct gcaacaaact ctgagaaaaa tttaatttgt
     4621 atatagcgtt gcacaaagct gaatgttaaa aatgcggatc caccagcaat gactgcaatc
     4681 caacaatctg atgcgacacc accaaacatg aacaggaaga agccacatgc aatggcagct
     4741 gcaaagaaat tcgttaaaaa agaatattgt aatgatgcat gctgtaaatg aataaattca
     4801 gatttagctt catcaattgt gagttcttta tttgatattt tacgtgaaag actattcgtt
     4861 aaagcgattt tctctaaatc tgttgtacgc tcttgtacac gaattaatct tgtacttgta
     4921 cgatcattta atgaaaaaat aattgcagtt gaactgacaa aactatatgt attatgaaga
     4981 ccataactat gtgcgatacg gttcattgta tcttcaactc gatatgtttc agcacctgat
     5041 tcaagtaaaa ttctacctgc aattaataca acatcaatca ctttgttttc atctataatt
     5101 gtgattgaat ctggcatatc aattcacctc caatgatatg tgttatttat ttgaacaatt
     5161 gaagtttaca acttgttgtt gcaactttca atagtgagac tttgtgttag tatgatgaac
     5221 ttgtatggtt caaatttaaa taagaaaaac tgttaatctt tgctattata ctatgattta
     5281 ataatagcaa aggattaaca gttttgtcgt tgttatagat tgatgatagg gttaaacatt
     5341 actttatttc ggccttgatt tttggctaca tgcaccatat cgtctgcatc tttaaacact
     5401 ttacgctgtg attttggatc gtcatctgtt aaataaccaa caccgataga cactgacaat
     5461 ttaataactt ctttgtttgg taaatggaat gatgattttt caacacccga acgaatattt
     5521 tcagctaatt taacactttg atcaagtgaa tagttgtgaa tgacaactga gaactcttcg
     5581 ccaccatttc taaaaatttt aaattgattc ggcacatagt ttttaagtaa ttgagacatt
     5641 tgttttaata cagcatcacc tgatttgtgt gagtaggtat cattgacatc tttaaatcca
     5701 tcgatatcga ttaataataa tgcgatactt tgatgttctt tttcagcttt tcgtgaaatt
     5761 tcatttaaat gtctatcaaa ttcttttaca ttacctaagc ctgttaagta atcatattta
     5821 tcttcgtttt cataacgatt tacgagtgag aagaaatgcc aaatatcgac aaatgttatc
     5881 gctgaagcta aagtgataat taatgaaatt ggtattaaaa tgataacttc cgatagtgtg
     5941 taaataggac tcactaatgc gacaccaaat aaaatgatta ttgtaacaac attaagtatt
     6001 aacaatgata gcacatcatt ttgttttaaa aatggtccaa tagcacttgt tactgcggca
     6061 ataacaatca acgtaacacc gtacataatc gagttgttaa atactacaat ttcaacaatt
     6121 gctacaatta ctgtggcaga taatgtatag accatatttg taaatctacc taaaaacaat
     6181 aaaggaacga atgttaagtg aattaaataa tcttcacgat aagggatagg gtagacagat
     6241 aataataatg atacgattgt cattaataca gtgacatatg ccttagaaaa aaccatacgt
     6301 ttgttttctg aatactgtaa gcgatggaat aaatagattc cagcgactat aacagatata
     6361 ttgtatataa atgcttcgaa catgtctgaa tcgactcctt taattgacca ctagctattg
     6421 taagtgaaaa cttacaattt gtcattagtt tacatataaa attaatgtat gatatagact
     6481 ttgatgttaa aatgttgcct taaatgatat gatgaaaaaa tgaataatag gcgatatata
     6541 agaaatgaat cgtatagttg taaatatgat atcattgatt gagcgaatta atttataata
     6601 aagctataag atatacgtag aaaatagata tatcattcta taaagacaat attaaataaa
     6661 tataacgtta aaaacaatta atatcgatga aggtgaataa atggttacat tattactagt
     6721 tgcagtaaca atgattgtca gtttgacgat aacaccaatt gttattgcaa tatcgaaaag
     6781 attaaattta gttgataaac caaattttag aaaagtacac actaaaccta tttcagttat
     6841 gggtggtacg gtgattctct tttcattttt aataggtatt tggattggtc atcctattga
     6901 aacagaaatc aaaccactta ttattggtgc gattattatg tacgtacttg ggcttgtaga
     6961 tgatatctac gatttgaaac cgtatataaa attggctggt caaattgctg ctgccttagt
     7021 agttgctttt tatggtgtga ctattgattt tatttcgttg ccaatgggta caactattca
     7081 ttttggattt cttagtattc caattactgt gatttggatt gttgctatta caaatgcaat
     7141 taacttaatt gatggactcg atggtttggc atcgggtgtt tctgcaatcg gactcattac
     7201 aataggattc attgcaattt tacaagctaa tattttcata acgatgattt gttgtgtttt
     7261 attaggctct ttaattgggt ttttatttta caatttccat cctgccaaaa tatttttagg
     7321 tgatagtggc gctttaatga ttggatttat catcggattc ctttctttac tcggattcaa
     7381 aaatattaca attattgcat tgttcttccc aattgttatc ttagcagttc cattcattga
     7441 taccttgttc gcaatgattc gacgtgtgaa aaaagggcag catataatgc aagctgataa
     7501 atcgcatttg catcataagt tattagcttt aggctacaca catagacaaa cggtattatt
     7561 aatctattca atttctattt tatttagtct ttcgagcatt atcttgtatg tatcgccacc
     7621 attaggtgtt gtattaatgt ttgtattaat catatttagt attgaattaa ttgttgaatt
     7681 tacaggatta atagataaca actaccgacc aatattaaat ttaattagtc gtaagtcatc
     7741 tcataaagag gaatagattt taaaacggta agtaataaaa taaaaaatga tgtctgtact
     7801 tttatgttgt tataaagaag gcatactcac aaaaaacact agcatggaac gtgctttcca
     7861 aattgcaaag taacaatacg caattagagt tagccatcct aagctagtgc tttttatttt
     7921 tattcagttg gtatatcgaa aggtaactgc tttggagttt cttcagtcaa atcgaaattt
     7981 ccagcagtca tttgatttaa aaaattaata aacgcttcat agtcactttt aacgacatcg
     8041 atatagtagc ttaccttatc agtgtaagtt tggtttctta acataaaatg agttgaggct
     8101 aattcatatt caaatttacc agtttgatca taattcagtg ttactataca tggtactgct
     8161 tctcgtagtt cgacacgtcc gatatcataa atgacgtctc tgacagcacc gctataggcg
     8221 cgaattaaac cgccaccacc taatttaata ccaccaaaat atcttgttac tacgacacac
     8281 gcattatgaa catcgagctt ttttaatatg tctaacattg ggacaccggc agttcctgtc
     8341 ggttcaccat catcattcgc tttttgaata ttcatttcag gtccaatagt atatgcagaa
     8401 caattatgag tggcatcttt atgttctttt tttattgcag caataaatgc tttagcttca
     8461 tcttcatttt gaacaggttt gatatgagca atgaatcttg atttactaat cacattttca
     8521 ataatgtgtt cttttttaac agtaatgata ttttgtgtca taataactcc ttaattcata
     8581 agcttaagat tatttaatct tcattataca ctgaaaatga tatgactata aatcgtttga
     8641 ttgccatttt ctttttaact gaaatattgt atcattgcta tgagtatatt ttaggaggac
     8701 gactatgaaa attgctgtga tgaccgattc tacaagttat ctgtcgcagg acttaatcga
     8761 taaatataat attcaaatag cgccattaag tgtgactttt gatgatggga agaactttac
     8821 agaaagtaat gaaatagcaa ttgaagaatt ttataataaa atggcatcgt ctcaaacgat
     8881 tccaacaaca agccaaccag caattggcga atggattact aaatatgaaa tgttaagaga
     8941 tcaaggttac acagatatca ttgtcatttg cttatcaagt ggaattagtg gaagttatca
     9001 atctagttat caagcagggg aaatggttga aggtgttaat gtacatgcat ttgatagtaa
     9061 gcttgcagca atgattgaag gatgttatgt attacgtgct attgaaatgg ttgaagaagg
     9121 atacgaacca cagcaaatta ttgatgattt aactaatatg cgtgaacaca caggtgcata
     9181 tttaattgtt gatgacttaa agaatttaca aaaaagtggt cgaattactg gtgctcaagc
     9241 atgggttgga acattattga aaatgaagcc agttcttaag tttgaagatg gcaagattat
     9301 accagaagaa aaagttcgta ctaaaaagcg tgccattcaa acattagaaa agaaagtatt
     9361 agatattgta aaagactttg aagaagtaac tttatttgtc ataaatggag accatttcga
     9421 agatggacaa gcgttataca aaaagttaca agatgattgt ccttcagctt atcaagtagc
     9481 atactctgag tttggtccag ttgttgcagc acatttaggt tcaggtggat taggtttagg
     9541 ctatgttggc agaaaaataa gattaacata attataaaat tttaataaaa gagtctatat
     9601 tgtaattgga aattatctct cgtatacatg gctttaaatg ttcatcattt gaatgtcaaa
     9661 atgctaaaga tataagaaaa tcattataat attagactct tttttacgtt gaaatgaggt
     9721 tttaagcatt aaacattacg ggaaattaat tcatcctcat acttcactta ctaatgaaaa
     9781 aattaataaa gaagtaacag gtgtcatcaa acaaaagtca aactattatt gtgttcaatg
     9841 tgaaagtaca aatccaaagc atttttatca gtatgattcc tcagtacatt ccaagaaaat
     9901 tgtatattgc agaaattgta tatcactggg tcgaatggat aatgtaacaa gatataaaat
     9961 aacagaaagt tcgcaaagtt catcacaagc atattatcat ctctcatttg aattatcgga
    10021 acaacagtct tatgcctcag aacatattgt tcgagccatt agaatgagac aaacgatttt
    10081 gttatatgca gtaacaggtg caggtaagac agaaatgatg tttcaaggca ttcaatatgc
    10141 aagacgacag ggagataata tagctattgt gtcaccacgt gtagatgttg ttgtagaaat
    10201 tagtaaacgt attaaagacg catttcttaa tgaagatata gacatactac accagcaatc
    10261 aagtcaacaa tttgaagggc attttgttgt atgcacagtg catcaacttt accgattcaa
    10321 acagcacttt gatactattt ttattgatga agtcgacgcc tttcctttat caatggataa
    10381 aagtttacaa caagcattga agtcatcttc taaagttgaa catgcaacga tttatatgac
    10441 agcaacacca ccgaaacaac ttctgtcaga gattcccctc gaaaatataa ttaaattgcc
    10501 agctcgcttt cataaaaaat cacttccagt tcctaaatat cgctatttca aacttaacaa
    10561 taataagatt cagaaaatgt tatatcgaat tttacaagat caaattaata atcaacgtta
    10621 tacactggtg ttttttaaca atatagaaac aatgattaaa acattttcga tttataagca
    10681 gaaaattact aaattaacat atgtccatag cgaggatgtt tttcgctttg aaaaagttga
    10741 acaattaagg aatggacaat acgatgtcat ttttactacg acaatattag aacgtggatt
    10801 tacaatggca aatttagatg ttgttgttat cgatgcacat caatatactc aagaggcttt
    10861 aatacaaatt gctggacgtg ttggacgaaa attagaatgt cctactggaa aagtattgtt
    10921 ttttcatgaa ggtgtaagta tgaatatgat tcaagctaaa aaagagattc aaaagatgaa
    10981 caaattagca ttaaaaagag gttggattga tgaataattg tttgagttgt ggtgctaagt
    11041 tatatgaaaa tataaccatt tataatttgt tcaagaaacc taatagatta tgtgacagat
    11101 gcaaagagaa ttgggacaat attaaacttg atattaaagc aaggcgatgt tcaaggtgct
    11161 taaaatactt agatcaaaat gaagcgtatt gtttagactg caagtttcta tcggcacact
    11221 ttaatttaat ggaacaatta tattgtcaat ttcaatatga cggtttaatg aaagagatga
    11281 tacatcagta taaattttcg aaagactatt atttatgtga attattggca catttgattg
    11341 aaataccaca aacatcttat gactatattg tgccaattcc ttcttcgccg gcacatgatt
    11401 tatctagaac atttaacccg gtagaagcag tactaaaagc aaaagggatt cgctttgata
    11461 agattttaaa gatgtcaaat agaccaaaac agtctcattt aactaagaaa gagcgtctgg
    11521 cagatgaaaa tccatttatt attgatacgg aattagattt aaatggtaag gaaatattac
    11581 tcgttgacga tatttataca actggattaa caattcatcg tgcagggtgt aaattatatg
    11641 ctaaaaatat cagaaaattc aaagtgtttg cgtttgcacg atagcgtaaa aatgttaaaa
    11701 tataataaag agttaccaat aaagaggttt aaggagagat tactatgatt agatttgaaa
    11761 ttcatggaga taacctcact atcacagatg ctattcgcaa ctatattgag gaaaaaattg
    11821 gtaagttgga acgttatttt aatgacgtac caaatgcagt ggcgcatgtt aaagttaaaa
    11881 cttattcaaa ttcagctact aaaattgaag taacaattcc attgaaaaat gttacgttaa
    11941 gagctgaaga gcgaaacgat gatttatacg caggtattga tttaattaat aataaacttg
    12001 aaagacaagt tcgaaaatat aaaacacgta ttaatcgtaa gagccgtgat cgaggagatc
    12061 aagaagtgtt tgttgccgaa ttacaagaaa tgcaagaaac acaagttgat aatgacgctt
    12121 acgatgataa cgagatagaa attattcgtt caaaagaatt cagcttaaaa ccaatggatt
    12181 cagaagaagc ggtattacaa atgaatctat taggtcatga cttctttgta ttcacagaca
    12241 gagaaactga tggaacaagt atcgtttacc gccgtaaaga cggtaaatat ggcttgattc
    12301 aaactagtga acaataaatt aagtttaaag cacttgtgtt tttgcacaag tgctttttta
    12361 tactctaaaa gcaatttcta acaatttcat agttcgataa tgtaatttgt tgaatgaaac
    12421 atagtgacta tgctaatgtt aatggatgta tatatttgaa tgttaagtta ataatagtat
    12481 gtcagtctat tgtatagtcc gagtcgaaaa tcgtaaaata tttataatat aatttattaa
    12541 gaagtataat tgcgttttga gaatatattt attagtgata aacttgttga caacagaatg
    12601 tgaatgaagt atgtcataaa tatatttata
//
LOCUS       DATTQF010000074        12586 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.074, whole genome shotgun sequence.
ACCESSION   DATTQF010000074 DATTQF010000000
VERSION     DATTQF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.074"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..455
                     /gene="holA"
                     /locus_tag="VB241_001673"
     CDS             <1..455
                     /gene="holA"
                     /locus_tag="VB241_001673"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP1244965.1"
                     /translation="QEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(500..751)
                     /locus_tag="VB241_001674"
     CDS             complement(500..751)
                     /locus_tag="VB241_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP1244966.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            1097..2920
                     /gene="lepA"
                     /locus_tag="VB241_001675"
     CDS             1097..2920
                     /gene="lepA"
                     /locus_tag="VB241_001675"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP1244967.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            3427..4551
                     /locus_tag="VB241_001676"
     CDS             3427..4551
                     /locus_tag="VB241_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP1244968.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSRRFKKKFDQSIESVFG
                     QTINNLKEKKLIVEKNDAIALTNRGKVIGNEVFEAFLIND"
     gene            4652..5629
                     /gene="hrcA"
                     /locus_tag="VB241_001677"
     CDS             4652..5629
                     /gene="hrcA"
                     /locus_tag="VB241_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HEP1244969.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5661..6287
                     /gene="grpE"
                     /locus_tag="VB241_001678"
     CDS             5661..6287
                     /gene="grpE"
                     /locus_tag="VB241_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP1244970.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            6356..8188
                     /gene="dnaK"
                     /locus_tag="VB241_001679"
     CDS             6356..8188
                     /gene="dnaK"
                     /locus_tag="VB241_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP1244971.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            8324..9463
                     /gene="dnaJ"
                     /locus_tag="VB241_001680"
     CDS             8324..9463
                     /gene="dnaJ"
                     /locus_tag="VB241_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP1244972.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINDQPSNFKDRAKRFFKGE"
     gene            9467..10405
                     /gene="prmA"
                     /locus_tag="VB241_001681"
     CDS             9467..10405
                     /gene="prmA"
                     /locus_tag="VB241_001681"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP1244973.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSYDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNELTYDDKLRQRIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASYLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIDDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            10407..11159
                     /locus_tag="VB241_001682"
     CDS             10407..11159
                     /locus_tag="VB241_001682"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP1244974.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAHEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            11166..12512
                     /gene="mtaB"
                     /locus_tag="VB241_001683"
     CDS             11166..12512
                     /gene="mtaB"
                     /locus_tag="VB241_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEP1244975.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEEEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
BASE COUNT         4713 a         1722 c         2418 g         3733 t
ORIGIN      
        1 cacaagagat agaaaagttg attttatttt taggcgatag accaacaatt aataagcagg
       61 atgttaacca aattattaat agaagtttag aacaaaatgt atttttactg actgaataca
      121 ttcagaaaag aaagaaagaa caagcaattc atttagtaaa agatttaata acaatgaaag
      181 aagaacctat taaattactt gcactaatta caagtaatta ccgattattt tatcaatgta
      241 agattctgag tcaaaaagga tatagtggac agcaaattgc taaaacaata ggcgtacatc
      301 catacagagt aaaattagcg ttaggacaag taagacatta tcaacttgat gaattattga
      361 atattataga tgcttgtgcg gaaactgatt ataaacttaa atcatcatat atggataaac
      421 agttaatact ggaattattc attctatctt tataaaaaat aaaagttcaa gcatgcgctt
      481 gaacttttat ttaaaaagat tatttatttg cagtcattaa ttgtgattta atacggtcag
      541 ctttgtttga atgtattaaa ttactttgag cagctttgtc tactaactta actgctaagc
      601 ttactaattc atttttatta tcagcgttat ttgaaacagc tgttttagcg tttttaactg
      661 ctgtacgcat tgcactcttt tgtgaaatgt tgcgtgcttc agctttttca gttgttttta
      721 cacgtttaat tgcagatttg atatttgcca tttctgtcac ctcctaaaag tgagttcatc
      781 ttatcaaaat tttatttgat tacaacaaga aatattttaa caaaagtcta accgttgtgc
      841 aatcatttat ttgatggggt tagcttaaaa ttttgcagat ttaataaatt gtgttggaaa
      901 cacaatcaat acgttttata attataagta cgtaattaat atattaacat atcgcaagta
      961 tgtatttaaa taaagattgt tataaattca aagttcatcc aagattatgg cgtttgcatt
     1021 tacctattaa aaacgttatt atatcaaaga tgcgaaagat aatacgggtt tattttatga
     1081 aagtgagaag gataaaatgg ataatgagca acgcttaaaa agaagagaga atataaggaa
     1141 tttctcgatt atagcacata ttgaccacgg aaaatctaca ttggctgata gaattttaga
     1201 aaataccaaa tcagttgaaa caagagatat gcaagatcag ttactagatt caatggattt
     1261 agaaagagaa cgtggtatta caatcaaatt aaacgcagta cgtttaaagt acgaagctaa
     1321 agatggaaat acttatacat tccatttaat cgatacgcct ggacacgtcg attttacata
     1381 tgaagtgtca cgttctttgg cagcttgtga gggcgcgatt ttagtagtag atgcggctca
     1441 aggtatcgaa gcacaaacat tagcaaatgt ttatttggca ttagataatg agttagagtt
     1501 attgcctgtt attaacaaaa ttgatttacc tgctgcagaa cctgaacgcg tgaaacaaga
     1561 aattgaagat atgataggtt tagaccaaga cgatgttgtt ttagcaagtg ctaaatctaa
     1621 cattggaatt gaagagatac ttgagaaaat agttgaagtt gtgccagctc cagatggtga
     1681 cccagaagca ccactaaaag cgttaatatt tgattctgag tatgatccat atagaggggt
     1741 aatttcatcg ataagaattg tagacggtgt tgttaaagcc ggagataaaa ttcgaatgat
     1801 ggccactggt aaagagttcg aagtaacaga agttggaatt aatacaccta agcaacttcc
     1861 agttgatgaa ttaacagttg gtgatgttgg ttacattatt gcaagtatta aaaatgttga
     1921 tgattctagg gttggtgaca caatcacatt agctagtaga cctgcatcag aaccattgca
     1981 aggttataag aaaatgaatc caatggtata ttgcggactg ttcccaatag ataacaaaaa
     2041 ttataatgat ttaagagaag cattagaaaa attacaattg aatgatgcat cattagaatt
     2101 tgagcctgaa tcatcacaag cattaggttt tggttataga actggtttct taggtatgtt
     2161 acacatggaa ataattcaag aaagaattga aagagaattt ggcattgaat taattgcaac
     2221 cgcaccatct gtaatttatc aatgtatttt aagggacggt tcagaagtga cggttgataa
     2281 tccagcacaa atgccagacc gtgataaaat tgataaaata ttcgagccat atgttcgtgc
     2341 aactatgatg gttccaaatg actatgtagg tgcagtaatg gaattatgtc aacgtaaacg
     2401 tggacaattt ataaatatgg actatttaga tgatattcgt gtaaatattg tttatgaatt
     2461 acctttagct gaagttgtat ttgatttctt cgatcaactt aaatctaata ctaaaggata
     2521 tgcatcattt gattatgagt tcatcgaaaa taaagaaagt aatttagtga agatggatat
     2581 tttattaaac ggtgataaag tggatgctct aagcttcata gtccatagag attttgcata
     2641 tgaacgtggt aaagcattag ttgaaaaact taaaacgtta attccaagac agcaatttga
     2701 agtacctgta caagctgcaa taggacaaaa aattgtagca cgtacaaata ttaaatcaat
     2761 gggtaaaaac gttttagcta aatgttatgg cggtgacata agccgtaaac gtaaattact
     2821 tgaaaaacaa aaagcaggta aagctaagat gaaagcagtt ggtaatgttg aaattccaca
     2881 ggatgctttt ttggctgtat tgaaaatgga tgacgaataa ttttttaaaa atcaattaac
     2941 aatttacaat gaataaagtt taataactaa aaagagggag cctaggataa attaacgtcc
     3001 tgggctttac aatgttatat tggcagtcat cgacagagtt aaaatgagct tataacaatg
     3061 gggccccaac atagaagctg acgaaaagtc agcttacaat aatgtgcaag ttggggtggg
     3121 acgacgaaat aaattttgcg aaaatatcat ttctgtccca ctcccttatg catgagttta
     3181 actcatgtaa ttctattttt aagtacatat tacctctggc taatgtgtaa gagccactac
     3241 ataataaatc attagtggtt ctttattatt tctatctcac tccctcttaa catttataaa
     3301 tattaaaatg aatcgatata ttagacaatt attgattaaa cgttaaagtt aaaagtaaga
     3361 ataattgcag atagtccaac aggatatagc cgattggata aacagtctga gaagcggggc
     3421 attaaaatga cagtacaaag tgcatatata catattccat tttgtgtaag aatatgtaca
     3481 tattgtgatt tcaataaata ttttatacag aaccaacctg tagatgagta cttagatgca
     3541 ctaataacag aaatgtctac agcaaaatat aggaacttaa agactatgta tgtaggtggc
     3601 ggcacaccaa cggccctttc tattaatcag ttggaaagat tacttaaagc aatacgtgat
     3661 acgtttacaa tcacaggtga gtatacattt gaagcaaatc ctgatgagtt aactaaagag
     3721 aaagtccaac tattagagaa atatggagta aacagaattt caatgggcgt tcaaacattc
     3781 aagccggagt tattgtctgt tttaggtaga acgcacaata ctgaagatat ttacacgtcg
     3841 gtgttaaatg ctaaaaacgc cggtattaaa tcaatcagtt tagatttaat gtatcattta
     3901 ccgaaacaga cgattgaaga ttttgaacaa agtttagatc tagctttaga tatggatatt
     3961 caacatattt cgagttacgg cttaatactt gaacctaaaa cccaatttta taatatgtat
     4021 agaaaaggtt tgctcaaact tcctaatgag gatttaggtg ctgacatgta tcagttgctg
     4081 atgtctaaga tagaacaatc tcctttccat caatacgaaa tatctaattt tgcattagat
     4141 ggccatgaat cagaacataa taaggtttac tggtttaatg aggaatatta tggatttgga
     4201 gcaggtgcaa gtggttatgt agatggtgtt cgttatacga atatcaatcc agtgaatcat
     4261 tatatcaaag ctataaataa agaaagtaaa gcaattttag tatcaaataa accttctttg
     4321 actgagagaa tggaagaaga aatgtttctt gggttgcgtt taaatgaagg tgtgagtagt
     4381 agaaggttca aaaagaagtt tgaccaatca attgaaagtg tctttggtca aacaataaat
     4441 aatttaaaag agaagaaatt aattgtagaa aagaacgatg cgattgcact tacaaatcga
     4501 gggaaagtca ttggtaatga ggtttttgaa gctttcctaa taaacgatta aaaaaaattg
     4561 aaatttcgag tctttaacat tgacttactt tgaccaattt gataaattat aattagcact
     4621 tgagataagt gagtgctaat gaggtgaaaa catgattaca gataggcaat tgagtatatt
     4681 aaacgcaatt gttgaggatt atgttgattt tggacaaccc gttggttcta aaacactaat
     4741 tgagcgacat aacttgaatg ttagtcctgc tacaattaga aatgagatga aacagcttga
     4801 agatttaaac tatatcgaaa agacacatag ttcttcaggg cgttcgccat cacaattagg
     4861 ttttaggtat tatgtcaatc gtttacttga acaaacatct catcaaaaaa caaataaatt
     4921 aagacgatta aatcaattgt tagttgagaa tcaatatgat gtatcatcag cattgacata
     4981 ttttgcagat gaattatcaa atatatctca atatacaact ttagttgttc atcctaatca
     5041 taaacaagat attatcaata atgtacactt gattcgtgct aatcctaatt tagttattat
     5101 ggttatcgta ttttcatcag gtcatgttga gcatgtacat cttgcttcag acataccgtt
     5161 tagtaatgac aaactaaata caatatcaaa ttttgttact aacaaactaa ctgagtttaa
     5221 tcagaattta caagatgata ttgtctcttt tgttcaatca gagcaggaag aaatatttat
     5281 caataaattg ttaaatacga tgaataatca tatttctaat caaagtaata gcatttatat
     5341 gggggggaag gttaaactta ttgatgcatt gaatgaaagt aatgtatctt caattcagcc
     5401 cattctacaa tatatagaat caaatagaat tgcagaatta ttacaagata tatcttcacc
     5461 aaatattaat gtaaaaattg gtaatgaaat tgatgacagc ttaagtgata tttcaattgt
     5521 aacaagtcaa tatcattttg atgaaacgtt aaaaggtcaa atcgctgtga ttggacctac
     5581 agctatgcat tatcaaaatg tcattcaatt attaaataga atttggtaat gatggattag
     5641 aaagtattgg aggacagaaa atgacaaata aagacgaatc agttgaaaaa aacactgaat
     5701 caacagttga agaaacaaac gtcaaacaaa atattgatga ttcagttgaa caagctgaag
     5761 aaagtaaagg tcatttacaa gatgaagcaa tagaagaaac gtctgacgag aatgttattg
     5821 aagaaataga tccaaaagat caaaaaatta atgaacttca acaattagca gatgaaaacg
     5881 aagagaaata tttaaggctc tacgctgagt ttgaaaatta taagcgtaga attcaaaaag
     5941 aaaatgaaat aaataaaaca tatcaagcac aacgtgtgtt aacagatatt ttaccagcaa
     6001 tagacaatat agaacgtgca cttcaaattg aaggtgatga tgagactttt aaatctcttc
     6061 aaaaaggtgt acaaatggtg catgaaagtt tgattaacgc actaaaagat aatggtcttg
     6121 aagttattaa aactgaaggt gaagcatttg atccaaatat tcaccaagct gtagttcaag
     6181 atgataaccc tgattttgaa tctggcgaaa tcactcaaga actacaaaaa ggatacaagc
     6241 ttaaagatag agtattaaga ccatcaatgg tcaaagtaaa ccaataactt aaatttggcg
     6301 aaaagacatt gtttaaaatt aaattaattt aatgattaaa tggaggaatt ttattatgag
     6361 taaaattatt ggtatagact taggtacaac aaattcatgt gtaacagtat tagaaggcga
     6421 tgagccaaaa gtaattcaaa accctgaagg ttcacgtaca acaccatctg ttgtagcttt
     6481 caaaaatgga gaaactcaag ttggtgaagt agctaaacgt caagctatta caaacccaaa
     6541 cactgttcaa tctattaaac gtcatatggg gactgattat aaagtagata ttgaaggtaa
     6601 atcatacaca ccacaagaaa tctcagctat gattttacaa aacttaaaaa atacagctga
     6661 aagctattta ggtgagaaag ttgacaaagc tgtaattaca gtacctgcat actttaacga
     6721 tgctgaacgt caagcaacta aagatgctgg taaaattgct ggtttagaag ttgagcgtat
     6781 cattaatgaa ccaacagctg cagcattagc atatggttta gacaaaactg ataaagacga
     6841 aaaagttctt gtttttgact taggtggcgg tacatttgac gtatctatcc tagaattagg
     6901 tgacggtgta ttcgaagtac tatcaacagc cggtgacaac aaacttggcg gtgatgattt
     6961 tgaccaagta attattgact acctagttgc agaattcaaa aaagaaaatg gcgtagactt
     7021 atctcaagat aaaatggcat tacaacgttt gaaagatgct gctgaaaaag ctaaaaaaga
     7081 cttatcaggt gtatcacaaa ctcaaatctc attaccattt atctcagctg gtgaaaacgg
     7141 tccattacac ttagaagtaa acttaactcg ttctaaattt gaggaattat cagattcatt
     7201 aattagaaga acaatggaac ctacacgcca agcaatgaaa gacgctggct taacaaactc
     7261 agatatcgat gaagttatct tagttggtgg atcaactcgt attccagcgg tacaagaagc
     7321 tgtcaaaaaa gaaatcggta aagaacctaa caaaggtgta aacccggacg aagtagtggc
     7381 tatgggagct gcaatccaag gtggcgttat cacaggtgac gttaaagacg tagtattatt
     7441 agacgtaaca ccactatctt taggtattga aattttaggt ggacgtatga atacgttaat
     7501 tgaacgtaac actacgattc ctacatctaa atctcaaatc tattcaacag cagtagataa
     7561 tcaaccatca gtagatgtac acgtattaca aggtgaacgt ccaatggctg cggataataa
     7621 aacacttggt agattccaat tgactgatat tccaccagct gaacgtggta aacctcaaat
     7681 tgaagtaacg tttgatatcg ataaaaacgg tattgtaaat gtaactgcaa aagacttagg
     7741 tacaaataaa gaacaaagaa ttacaattca atcaagttct tcattatcag acgaagaaat
     7801 cgaccgtatg gtaaaagatg ctgaagttaa cgctgaagca gataaaaaac gtcgtgaaga
     7861 agtagactta agaaacgaag ctgacagcct agtattccaa gttgaaaaaa ctttaactga
     7921 tttaggcgaa aatatcggtg aagaagataa aaaatctgct gaagagaaaa aagacgctct
     7981 taaaactgct ttagaaggtc aagatataga agatattaaa tctaaaaaag aagaacttga
     8041 aaaagtgatt caagaattat cagcaaaagt atatgagcaa gcggctcaac agcaacaaca
     8101 agcacaaggt gctaatgctg gtcaaaacaa cgatagtact gtagaagatg ctgaatttaa
     8161 agaagtaaaa gacgacgaca aaaaataatt aaacaataca attagaatgt tgtcatgatc
     8221 tgaaaagtca aagtcaatag aacattggct ttggcttttt atttggatag tatgacatat
     8281 tacgttaaac tatatgagtt aattaagaaa aggagagata gctgtggcca aaagagacta
     8341 ttatgaagtt ttaggcatta gtaaagatgc ttcaaaagat gaaatcaaaa aagcgtatcg
     8401 aaagctttcg aaaaaatatc atccagatat taacaaagaa gaaggtgcag atgaaaagtt
     8461 taaagaaatt tctgaagcct atgaagtttt aagtgatgat aataaacgcg caagctatga
     8521 tcaatttgga catgacgggc cacaaggttt tggtggacaa ggattcaatg gctctgactt
     8581 tggcggtttt agtggcttcg gtggcggcgg ttttgaagat atctttagct ctttcttcgg
     8641 tggcggcaga caaagagatc caaatgcacc gcaaaaaggt gatgatcttc agtacacaat
     8701 gacattgaca tttgaagaag cggtatttgg tacaacaaaa gaaatttcaa tccgtaaaga
     8761 tgtaacatgc gaaacatgtc atggtgatgg tgcaaagcct ggaacaagta aaaagacttg
     8821 tagttactgt aatggcgctg gtcatgtagc tgtagaacaa aatacaattt taggtagagt
     8881 tcgcactgaa caagtttgtc ctaaatgtaa tggaagtggt caagaatttg aagaagcttg
     8941 tccaacatgt cacggaaaag gtactgaaaa taaaacggtt aaattagaag tgaaagtacc
     9001 tgaaggtgtc gacaatgaac aacaaattag attagctggt gaaggctctc caggcgtgaa
     9061 tggaggacct gctggtgatt tatacgtagt atttagagtt aaaccatctg aaacatttaa
     9121 acgtgatggt gatgatattt attacaaatt aaatgttagc ttcccacaag cagctttagg
     9181 cgatgaaata aaaataccaa cattaaataa tgaagttatg ttaacaatcc cagctggtac
     9241 acaaacaggt aagcaattcc gcttaaaaga aaagggtata aaaaatgttc atggctatgg
     9301 atacggagat ttatatgttg atattaaagt agtaacacct actaaattaa cagatagaca
     9361 aaaagaacta atgaaagaat ttgcccaatt aaatggtgaa gaaattaatg atcaaccttc
     9421 gaattttaaa gatagagcaa aaagattctt taagggagaa taaagtatga actggacaga
     9481 gctttcaatt attattaatc atgaagcagt agaattggct accaatatac ttgaaaatca
     9541 tggatcaaat ggtgtcgtga tagaagattc atatgattta attaatcaac cagaagataa
     9601 atacggtgaa atttacgctt tgaaaaaaga ggattatcca gataagggag taagattgaa
     9661 agcctatttt aatgagttga cttatgatga taagttgcga cagcgaatta aagatgagtt
     9721 attaaactta gatgaacttg atcaacataa cgttcaattc agtgagcaaa tcattgcaga
     9781 aacggattgg gaaaatgaat ggaaaaacta tttccatcca ttccgagcgt cgaagaagtt
     9841 cacaatagtc cctagttggg aaacatatgc taaagaagcg gatgaagagc tttgcattga
     9901 gctcgatcca ggtatggctt ttggaacagg agatcatccg actacaagta tgtgtttgaa
     9961 ggcaatagaa acatatgtat tgccacagca ttcagtaatt gatgttggta ctggctcagg
    10021 tatattaagt attgcaagtt atctaatcgg tgtaaaacgt attaaagcgt tggatattga
    10081 tgaaatggca gtgagtgtag ctaaagaaaa cttcagaaga aatcattgtg aaacgttaat
    10141 tgaagctgtt ccaggtaact tattgaaaga cgaaacagaa aaatttgata ttgtaatagc
    10201 aaatatttta gcgcatatta ttgatgaaat gattgatgat gcttataata ctctaaatga
    10261 aggcggttat tttattactt ctggtattat aaaagagaag tatgaaggta tacagtcaca
    10321 tatggagcgt gtaggtttta aaattatttc agaacaacat gacaacggtt gggtttgtct
    10381 tgttggccag aaagtgagtg aataatgtgc aacgttattt catagaccaa aacgctgatg
    10441 taagtcagcg tttttttatt acaaaaaaag aagatattca tcatataaca aatgtaatga
    10501 gatatgatgt tggtagtaaa attattttaa cttttaatga tcaaacagtt tatttatgtg
    10561 aaattgctga tataacaagt gataaaattg cagttgattt aaaagaaaaa caaaatatta
    10621 atacagaatt gccagttgat gttacgattt gcagtggact aattaaagct gacaaatatg
    10681 agtggttgct acaaaaagct actgaattgg gtgcttcatc gtttatagct gttagcatgg
    10741 aacgttcaat tgttaaatta aacgaggcaa aagttgccaa aaagatagaa cgttggcaaa
    10801 aaataattaa agaagctgct gaacaaagct atcgtttagt gattccatct attcaattcg
    10861 agtcgaattt aaaattaatt tgtgatacta ttgataatta tgactatatt cttattgcac
    10921 atgaagagga agcaaaagat ggtgagttaa gcaatttcaa gcaaacttta caacaattca
    10981 aggctcagga taaagtgttg atgatttttg gtcctgaggg tggtttgtca gaaaatgaaa
    11041 tttcattatt tagtaatact agtacagttg ttggtcttgg accgagaatt ttacgggcag
    11101 aaactgcgcc attgtatgcg ttaagtgcaa taagttatga aaaagaatta atggggtgaa
    11161 tatttatgtc aacagttgcg tttcacacat taggttgtaa agtaaaccat tatgaaactg
    11221 aagcaatctg gcaattattt aaagaagcaa actatgagcg cgttgacttt gaagcgaatg
    11281 ctgatgtatt tgttattaat acttgtacag taacgaatac aggtgataaa aaaagtcgtc
    11341 aaattattag acgtgcaata agacaaaatc ctgatgctgt aatctgtgta acaggttgtt
    11401 atgcgcaaac ttcatcagct gaaattatgg aaattcctgg tgtcgatgta gtagttggta
    11461 cacaagatag acataaacta ttaggttaca ttgatgaatt ccgtaaagaa cgccaaccaa
    11521 ttaatggtgt tggaaatatc atgaaaaatc gtaaatatga agaattagat gtcccttatt
    11581 ttacagatag aacacgtgcg tcattaaaaa ttcaagaagg ttgtaacaac ttttgcacat
    11641 tctgtattat tccatgggct cgtggcttaa tgcgttcaag agatccggaa aaagtagttg
    11701 aacaagcgac gcaactagtg aattcaggat ataaggaaat tgtattgacg ggaattcata
    11761 caggtggata tggtcaagat ttaaaagatt ataacttggc ccaattatta cgtgatcttg
    11821 aaacgattaa tggattagaa cgaattcgaa tttcttcaat tgaagcaagt caacttacag
    11881 atgaagtgat tgacgtttta gaacgttcaa caaaagttgt gcgtcatttg catattccgt
    11941 tacaatctgg ttcagataca gtattaaaac gtatgagacg taagtataca atggataggt
    12001 tttcggaacg attaacaaaa ttgcataaag ctttaccaga cttggcagtt acgagtgatg
    12061 taattgttgg tttcccaggt gaaactgaag aagagttcca agaaacatat gattttatcg
    12121 taaagcataa gttctctgaa ctgcatgttt tcccttattc tcctagaatt ggcacgccag
    12181 ctgcaagaat ggatgaccaa attgatgaag aaattaaaaa tgaacgcgtg cataagttaa
    12241 ttacgcttag caatcaactt ggaaagttat atgcgtctaa atttgatcaa gatgtgcttg
    12301 aagtaattcc tgaggagcag ggtgacacag aaggtacatt agttggatat gcagataatt
    12361 atatgaaagt acaatttgaa ggtgacgaat cactcatagg tcaaattgta aaagttaaaa
    12421 ttacgcaagc aaattatcca ttaaatgaag ggcaagcaat taaagttgtt gatttcgcaa
    12481 caaataaatc tgatagagaa gttttagttt aagtattcct tgattcatac cataaggttc
    12541 cgtattaaag atataggttt tgtaacgttg ttttaacagc ttcaca
//
LOCUS       DATTQF010000075        12161 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.075, whole genome shotgun sequence.
ACCESSION   DATTQF010000075 DATTQF010000000
VERSION     DATTQF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.075"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            370..1707
                     /locus_tag="VB241_001684"
     CDS             370..1707
                     /locus_tag="VB241_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEP1244976.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILKSMKQVEKHSDYDGYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLVVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRENIIEYLSYFQTSSPSYLIMASLESAAQFYKTYDSTLFFAKRAQLI
                     ECLENKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1709..2326
                     /locus_tag="VB241_001685"
     CDS             1709..2326
                     /locus_tag="VB241_001685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP1244977.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2354..2683
                     /locus_tag="VB241_001686"
     CDS             2354..2683
                     /locus_tag="VB241_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEP1244978.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2897..3823
                     /locus_tag="VB241_001687"
     CDS             2897..3823
                     /locus_tag="VB241_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP1244979.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNALALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDSDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3824..4627
                     /locus_tag="VB241_001688"
     CDS             3824..4627
                     /locus_tag="VB241_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEP1244980.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVELDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4644..4991
                     /gene="yabA"
                     /locus_tag="VB241_001689"
     CDS             4644..4991
                     /gene="yabA"
                     /locus_tag="VB241_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP1244981.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5265..5990
                     /locus_tag="VB241_001690"
     CDS             5265..5990
                     /locus_tag="VB241_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP1244982.1"
                     /translation="MLKENERFDQLIKENFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5983..6231
                     /locus_tag="VB241_001691"
     CDS             5983..6231
                     /locus_tag="VB241_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP1244983.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6233..7072
                     /gene="rsmI"
                     /locus_tag="VB241_001692"
     CDS             6233..7072
                     /gene="rsmI"
                     /locus_tag="VB241_001692"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP1244984.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIN"
     gene            7357..9330
                     /gene="metG"
                     /locus_tag="VB241_001693"
     CDS             7357..9330
                     /gene="metG"
                     /locus_tag="VB241_001693"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP1244985.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMRDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMINKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTEP
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9361..10134
                     /locus_tag="VB241_001694"
     CDS             9361..10134
                     /locus_tag="VB241_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP1244986.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10301..10837
                     /gene="rnmV"
                     /locus_tag="VB241_001695"
     CDS             10301..10837
                     /gene="rnmV"
                     /locus_tag="VB241_001695"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP1244987.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10848..11741
                     /gene="rsmA"
                     /locus_tag="VB241_001696"
     CDS             10848..11741
                     /gene="rsmA"
                     /locus_tag="VB241_001696"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP1244988.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEIGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVIIQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11841..12104
                     /locus_tag="VB241_001697"
     CDS             11841..12104
                     /locus_tag="VB241_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEP1244989.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
BASE COUNT         4449 a         1610 c         2397 g         3705 t
ORIGIN      
        1 tgtctaaaac gttgaaataa aggttaacac aaagaaaaat ggcttggcga agtgtgaaat
       61 tgataattta ataatgtgca cttttgattg tttaaatctg tgactagaag tataagcgaa
      121 gacattcaga agtattataa aaagtgaaca gcagtaagat agtttttaat cataaatcat
      181 cttactgctg tttttaggtt ttatgtctaa tatcttttaa atcgaaatac aaaaaggaaa
      241 ttaattatta tacaatagac aagctattgc ataagtaaca ctaacttttg tcaaagaagt
      301 gttactatat aattaatact tttgaaagta actaattcca aaacattgta aacaaaggaa
      361 gcgtatatca tgaagcaacc tattttaaat aaattagaaa gtttaaatca agaagaagcg
      421 atttctttgc atgttccggg tcataaaaat atgactatcg gtcatttatc tcaattatca
      481 atgacaatgg ataaaactga aatacctgga ttagatgatt tacatcatcc tgaagaagtc
      541 attttgaaaa gtatgaagca ggtggagaaa cattcagatt atgatggtta tttcttagtg
      601 aatggcacca cttcgggaat attatctgtc atccagtctt tttcacagaa aaaaggcgat
      661 atcttaatgg caagaaatgt acataaatct gtattacatg cgctcgatat tagccaacaa
      721 gaagggcatt ttattgaaac gcatcaaagt ccgttaacga atcattataa taaagttaat
      781 ttaagccgtt tgaataatga cggtcacaaa cttgttgtgt tgacttatcc taactattac
      841 ggtgaaacat ttaatgtaga agaggttatc aaatctttgc accaattaaa tattcctgta
      901 ctcattgacg aagcacacgg cgcgcacttt ggattgcaag gatttccaga ttctacatta
      961 aattatcaag ctgactatgt tgttcaatct tttcataaaa cgttaccagc tttaacgatg
     1021 ggctcggtac tttatattca taaaaatgca ccttatagag aaaatattat agaatatcta
     1081 agctacttcc aaacatctag tccttcatat ttgattatgg ctagtttaga gtcagctgcc
     1141 cagttctata aaacatatga tagtaccttg ttttttgcta agagagcgca attaatcgaa
     1201 tgtttggaga ataaaggttt tgaaatgctt caagttgatg atccgttgaa gctgttgata
     1261 aaatatgaag gctttacagg tcatgatatt caaaattggt ttatgaatgc acatatctat
     1321 ttagaattag cggacgacta tcaagcatta gcgatattgc cgttatggca tcatgatgat
     1381 acgtatttat ttgattcgct tttacgtaaa attgaagata tgattttacc gaaaaaatca
     1441 gtttctaaag ttaaacaaac acaactttta acaactgaag gtaactataa accaaaacgc
     1501 tttgaatatg ttacttggtg tgatttgaaa aaggcaaaag gtaaagtttt ggcgcgacat
     1561 attgtcccgt atccgccagg gattcctatt attttcaaag gagaaacaat aactgaaaat
     1621 atgatagaat tggtaaatga atatctggaa actggaatga tagttgaagg aattaaaaat
     1681 aataaaattt tagttgagga tgaataaaat gtcagctttt ataacttttg agggcccaga
     1741 aggctcaggt aaaacaactg taattaatga agtttatcat agattagtaa aagattatga
     1801 tgtcattatg acaagagaac cgggcggcgt tcctactggt gaagaaatac gtaaaattgt
     1861 attagaaggc aatgatatgg acattagaac tgaagcgatg ttatttgctg catctagaag
     1921 agaacatctt gtattaaagg tcataccagc tttaaaagaa ggtaaggttg tgttgtgtga
     1981 tcgctatatc gatagttcat tagcttatca aggttatgct agagggattg gcgttgaaga
     2041 agtaagagca ttaaatgaat ttgcaataaa tggattatat ccagatttga cgatttattt
     2101 aaatgttagt gctgaagtag gtcgcgaacg tattattaaa aattcaagag atcaaaatag
     2161 attagatcaa gaagatttaa agtttcacga aaaagtaatt gaaggttacc aagaaatcat
     2221 tcataatgaa tcacaacggt tcaaaagcgt taatgcagat caacctcttg aaaatgttgt
     2281 tgaagacacg tatcaaacta tcatcaaata tttagaaaag atatgatata attgttagaa
     2341 gaggtgttat aaaatgaaaa tgattatagc gatcgtacaa gatcaagata gtcaggaact
     2401 tgcagatcaa cttgttaaaa ataactttag agcaacaaaa ttggcaacaa caggtgggtt
     2461 tttaagagca ggtaatacaa cattcttatg tggtgtcaat gatgaccgtg tagatgaaat
     2521 attgtctgtg attaatcaaa cgtgtggtaa tagagaacag ttggtttcac ctattacacc
     2581 tatgggaggc agtgcggatt cgtacattcc atatccagtt gaagttgaag ttggcggtgc
     2641 tactgtattt gttatgccag ttgatgcatt ccatcaattt taattctata atacaatcat
     2701 caattagaga tacttaaaat agtgtattaa taagttatta acaattttgg gttgcttgac
     2761 tgcgactagt tcagatgcca atagatttga tttttgtggt tctaagaata atcacaaatc
     2821 atggtcgcta ttgttgcagt aattagttgc tctttggcaa cctttttata taaaagcaaa
     2881 agggagtttg taatgaatgg atgaacagca acaattgacg aatgcatatc attcaaataa
     2941 attatcgcat gcctatttat ttgaaggtga tgatgcacaa acgatgaaac aagttgcgat
     3001 taattttgca aagcttattt tatgtcaaac agatagtcaa tgtgaaacaa aggttagtac
     3061 atataatcat ccagacttta tgtatatatc aacaactgag aatgcaatta agaaagaaca
     3121 agttgaacaa cttgtgcgtc atatgaatca acttcctata gaaagcacaa ataaagtgta
     3181 catcattgaa gactttgaaa agttaactgt tcaaggggaa aacagtatct tgaaatttct
     3241 tgaagaacca ccggacaata cgattgctat tttattgtct acaaaacctg aacaaatttt
     3301 agacacaatc cattcaaggt gtcagcatgt atatttcaag cctattgata aagaaaagtt
     3361 tataaataga ttagttgaac aagacatgtc taagccagta gctgaaatga ttagtactta
     3421 tactacgcaa atagataatg cactggcttt aaatgaagaa tttgatttat tagcattaag
     3481 gaaatcagtt atacgttggt gtgaattgtt gcttactaat aagccaatgg cacttatagg
     3541 tattattgat ttattgaaac aggctaaaaa taaaaaactg caatctttaa ctattgcagc
     3601 tgtgaatggt ttcttcgaag atatcataca tacaaaggta aatgtagagg ataaacaaat
     3661 atatagtgat ttaaaaaatg atagtgatca atatgcgcaa aagttgtcgt ttaatcaatt
     3721 aattttgatg tttgatcaac tgacggaagc acataagaaa ttgaatcaaa atgtaaatcc
     3781 aacgcttgta tttgaacaaa tcgtaattaa gggtgtgagt tagatgccaa atgtaatagg
     3841 tgttcagttt caaaaagcgg gaaaattaga atattataca cctaatgata tacaagtaga
     3901 gttagatgac tgggtagttg tcgaatctaa aagaggcata gagataggta ttgttaaaaa
     3961 tccattaatg gatattgctg aagaggatgt tgtgttacct cttaaaaata ttattcgcat
     4021 tgctgatgac aaagatattg ataaatttaa ttgtaatgaa cgcgatgctg aaaatgcatt
     4081 aatactatgt aaagacattg taagagaaca aggtttggac atgcgtttag tcaattgcga
     4141 atatacatta gataaatcga aagttatttt taattttacg gcggatgatc gtattgattt
     4201 tagaaaatta gtaaaaatat tagcgcaaca tttaaaaaca cgtatcgagt tgagacaaat
     4261 tggtgtaagg gatgaagcca aattgcttgg cggtatcgga ccttgtggta gatcgttatg
     4321 ttgttctaca tttttaggtg attttgaacc agtatcgatt aagatggcta aggatcaaaa
     4381 tttatcatta aatccaacta aaatttccgg tgcatgtggt cgtttgatgt gttgtttaaa
     4441 atatgaaaat gactattatg aggaagtacg tgcgcaatta cctgatatcg gtgaagcaat
     4501 tgaaacgcct gatggtaacg ggaaagtagt tgctttaaat atattagaca tttctatgca
     4561 ggtgaagctt gagggacatg aacagccact tgaatataaa ttagaagaaa tagaaactat
     4621 gcattaagga ggcattatta catttggatc gcaatgaaat atttgaaaaa ataatgcgtt
     4681 tagaaatgaa tgtcaatcaa ctttcaaagg aaacttcaga attaaaggca cttgcagttg
     4741 aattagtaga agaaaatgta gcgcttcaac ttgaaaatga taatttgaaa aaggtgttgg
     4801 gcaatgatga accaactact attgatactg cgaattcaaa accagcaaaa gctgtgaaaa
     4861 agccattacc aagtaaagat aatttggcta tattgtatgg agaaggattt catatttgta
     4921 aaggcgaatt atttggaaaa catcgacatg gtgaagattg tctgttctgt ttagaagttt
     4981 taagtgatta atcaagcaca ctcaaatagt gttataatta taaatgaata tggtttggat
     5041 aagtctgaga caatgcatgt ttcaggcttt aattgtgtat aaagttttgg tgattgcata
     5101 agagatagcg gtactaaatg ttattattaa gtgtgcacgc agtatcatta gttataaaat
     5161 gtagctgtta aaagtcaaaa atacatcgaa tgtagatagg catataatat aaaaagagat
     5221 ttcaattact caatagaaaa aggttgtctt cataggagtt aaaaatgtta aaagagaatg
     5281 aacgatttga tcaactaatc aaagaaaatt ttagtattat tcaaaatgat gatgtttttt
     5341 cgttttcaac ggatgctttg ttgttaggac attttacaaa acctagaaca aaagatatag
     5401 tgttggactt atgttcaggc aatggggtga tacccttgtt attgtttgcg aaacatccac
     5461 gacatataga aggtgttgag attcaaaaaa cacttgtcga tatggcgcga cgcacatttc
     5521 aattcaatga tgttgatgaa tatttaacaa tgcatcacat ggatttgaaa aacgttacta
     5581 aagtatttaa accttcacaa tatactttag taacgtgtaa tccgccttat tttaaagaga
     5641 atcagcaaca ccaacatcaa aaagaagcac ataagatagc gagacatgag attatgtgta
     5701 cacttgaaga ttgcatgatt gcagcccgtc atttattaaa agaaggtggc aggctaaata
     5761 tggtacatcg tgcagataga ctcatggatg tcttgtttga aatgagaaaa gtaaatattg
     5821 aacctaagaa agtcgttttt atatatagta aagtagggaa atcggcacaa acgatagttg
     5881 tagaaggtcg aaaaggtgga aatcaaggtt tagaaatcat gcccccattt tatatttata
     5941 atgaagatgg taattatagc gaagaaatga aggaagtata ttatggatag tcattttgta
     6001 tatattgtaa aatgtagtga tggaagttta tatacaggat acgctaaaga cgttaatgca
     6061 cgtgttgaaa aacataaccg aggtcaagga gccaaatata cgaaagtaag acgtccggtg
     6121 catttagttt atcaagaaat gtatgagaca aagtctgaag cattgaagcg tgaatatgaa
     6181 attaaaactt ataccagaca aaagaaattg cgattaatta aggagcgata gtatggctgt
     6241 attatattta gtgggcacac caattggtaa tttagcagat attacttata gagcagttga
     6301 tgtattgaaa cgtgttgata tgattgcttg tgaagacact agagtaacta gtaagctgtg
     6361 taatcattat gatattccaa ctccattaaa gtcatatcac gaacataaca aagataagca
     6421 gactgctttt atcattgaac agttagaatt aggtcttgac gttgcgctcg tatctgatgc
     6481 tggattgccc ttaattagtg atcctggata cgaattagta gtggcagcca gagaagctaa
     6541 cattaaagta gagactgtgc ctggacctaa tgctgggctg acggctttga tggctagtgg
     6601 attaccttca tatgtatata catttttagg atttttgcca cgaaaagaga aagaaaaaag
     6661 tgctgtatta gagcaacgta tgcatgaaaa tagcacatta attatatacg aatcaccgca
     6721 tcgtgtgaca gatacattaa aaacaattgc aaagatagat gcaacacgac aagtatcact
     6781 agggcgtgaa ttgactaaga agttcgaaca aattgtaact gatgatgtaa cacaattaca
     6841 agcattgatt cagcaaggcg atgtaccatt gaaaggcgaa tttgttatct tgattgaggg
     6901 tgctaaagcg aataatgaga tatcgtggtt tgatgattta tctatcaatg agcatgttga
     6961 tcattatatt caaacttcac agatgaaacc aaaacaagct attaaaaaag ttgctgaaga
     7021 acgacaactt aaaacgaatg aagtatataa tatttaccat caaataaatt aatcacttta
     7081 tcgattatat gaaattttaa acgattttat aaacgcaagc tgtaatttta aatggtaagt
     7141 tatcattttg cattgatact gataaaatga tgttgactat gataaaaaaa tgatgacatc
     7201 gacgtttttt aatgtaaaat aaatacattg aaagtaataa ataccttaac attgaataag
     7261 atgaaaatga gatgacgaga taaatgttcg cgtccgttga aatgcataga aatcttagat
     7321 attatttgaa gtgagacatt acgaggagga acagttatgg ctaaagaaac attttatata
     7381 acaaccccaa tatactatcc tagtgggaat ttacatatag gacatgcata ttctacagtg
     7441 gctggagatg ttattgcaag atataagaga atgcaaggat atgatgttcg ttatttgact
     7501 ggaacggatg aacacggtca aaaaattcaa gaaaaagctc aaaaagctgg taagacagaa
     7561 attgaatatt tggatgagat gattgctgga attaaacaat tgtgggctaa gcttgaaatt
     7621 tcaaatgatg attttatcag aacaactgaa gaacgtcata aacatgtcgt tgagcaagta
     7681 tttgaacgtt tattaaagca aggtgatatc tatttaggtg aatatgaagg ttggtattct
     7741 gttccggatg aaacatacta tacagagtca caattagtag acccacaata cgaaaacggt
     7801 aaaattattg gtggtaaaag tccagattct ggacatgaag ttgaactagt aaaagaagaa
     7861 agttatttct ttaatattag taaatataca gatcgtttat tagagttcta tgaccaaaat
     7921 ccagatttta tacaaccacc atcaagaaaa aatgaaatga ttaacaactt cattaaacca
     7981 ggacttgctg atttggctgt ttctcgtaca tcatttaact ggggtgtcca tgttccgtct
     8041 aatccaaaac atgttgttta tgtttggatt gatgcgttag ttaactatat ttcagcatta
     8101 ggctatttat cagatgatga gtcactattt aacaaatact ggccagcaga tattcattta
     8161 atggctaagg aaattgtgcg attccactca attatttggc ctattttatt gatggcatta
     8221 gacttaccgt tacctaaaaa agtctttgca catggttgga ttttgatgag agatggaaaa
     8281 atgagtaaat ctaaaggtaa tgtcgtagac ccgaatattt taattgatcg ctatggttta
     8341 gatgccacac gttattatct aatgcgtgaa ttaccatttg gttcagatgg cgtatttaca
     8401 cctgaagcat ttgttgagcg tacaaatttc gatctagcaa atgacttagg aaacttagta
     8461 aaccgtacga tttctatgat taataagtac tttgatggcg aattaccagc gtatcaaggt
     8521 ccacttcatg aattagatga agaaatggaa gctatggctt tagaaacagt gaaaagttat
     8581 actgaaagca tggaaagttt gcaattttct gtggcattat ctacggtatg gaagtttatt
     8641 agtagaacga ataagtatat tgacgaaaca acgccttggg tattagctaa ggacgatagc
     8701 caaaaagata tgttaggcaa tgttatggct cacttagttg aaaatattcg ttatgcagct
     8761 gtattattgc gtccattctt aactcatgcg ccgaaagaga tttttgaaca attgaacatt
     8821 aacaatcctc aatttatgga atttagtagt ttagagcaat atggtgtgct tactgagcca
     8881 attatggtta ctgggcaacc taaacctatt ttcccaagat tggatagcga agcggaaatt
     8941 gcatatatca aagaatcaat gcaaccgcct gctactgaag aggaaaaaga agagattcct
     9001 agcaaacctc aaattgatat taaagacttt gataaagttg aaattaaggc agcaacgatt
     9061 attgatgctg aacatgttaa gaagtcagat aagcttttaa aaattcaagt agacttagat
     9121 tctgaacaaa gacaaatcgt atcaggaatt gccaaattct atacaccaga tgatattatt
     9181 ggtaaaaaag tagcagttgt tactaacttg aaaccagcta aattaatggg acaaaaatct
     9241 gaaggtatga tattatctgc tgaaaaagat ggtgtattaa ccttagtaag tttaccaagt
     9301 gcaattccaa atggtgcagt gattaaataa ctgtattttt aaaaattagg agagataatt
     9361 atgttaatcg atacacatgt ccatttaaat gatgagcaat acgatgatga tttgagtgaa
     9421 gtgattacac gtgctagaga agcaggtgtt gatcgtatgt ttgtagttgg ttttaacaaa
     9481 tcgacaattg aacgcgcgat gaaattaatc gatgagtatg attttttata tggcattatc
     9541 ggttggcatc cagttgacgc aattgatttt acagaagaac acttggagtg gattgaatct
     9601 ttagctcagc atccaaaagt gattggtatt ggtgaaatgg gattagatta tcactgggat
     9661 aaatctcctg cagatgttca aaaggaagtt tttagaaagc aaattgcttt agctaagcgt
     9721 ttgaagttac caattatcat tcataaccgt gaagcaacac aagactgtat cgatatctta
     9781 ttggaggagc atgctgaaga ggtaggcggg attatgcata gctttagtgg ttctccagaa
     9841 attgcagata ttgtaactaa taagctgaat ttttatattt cattaggtgg accagtgaca
     9901 tttaaaaatg ctaaacagcc taaagaagtt gctaagcatg tgtcaatgga gcgattgctt
     9961 gttgaaaccg atgcaccgta tctttcgcca catccatata gagggaagcg aaatgaaccg
    10021 gcgagagtaa ctttagtagc tgaacaaatt gctgaattaa aaggcttatc ttatgaagaa
    10081 gtgtgcgaac aaacaactaa aaatgcagag aaattgttta atttaaattc ataaagttaa
    10141 aagtgagaaa gatcaccgcc ataaatgtaa acgatgctat attcgtttaa tatgctatgg
    10201 ttctttctca cttttttaaa ttaaaatatc gtgcatgtgc aatacgtgcg atagagatgg
    10261 ttagagcttt gaaattaaga attgtaggaa ggcgttttaa atgaaaatca atgagtttat
    10321 agttgtagaa ggacgagatg atactgagcg tgttaaacga gctgttgaat gtgatacgat
    10381 tgaaacgaat ggtagtgcca tcaacgaaca aactttagaa gtaattagaa atgctcaaca
    10441 aagtcgaggc gttattgtat taacagatcc agatttccca ggagataaaa ttagaagtac
    10501 aattactgaa catgtcaaag gtgttaaaca tgcgtatatt gatagagaaa aagctaaaaa
    10561 taaaaaaggg aaaattggtg ttgaacatgc cgacttaatt gatattaaag aagcgttaat
    10621 gcatgttagt tcaccctttg atgaagctta tgaatcaatt gataaatctg tgctaataga
    10681 gttggggtta atcgttggga aagatgcaag gcgccgtaga gaaattttaa gtagaaaatt
    10741 gcgaatcggc cattccaatg gtaagcagtt attgaaaaag ttaaatgcat ttggttatac
    10801 cgaagcggat gtaaggcaag ctttagaaga tgaatgagga agtgaaaatg ttggataata
    10861 aagatattgc aacaccatca agaacgcgag cgttgttaga taaatatggc tttaatttta
    10921 aaaaaagttt aggacagaac tttttaatag atgtgaatat cattaataat atcattgatg
    10981 caagtgatat tgatgcacaa actggggtga ttgaaattgg tccaggtatg gggtcgttga
    11041 cagaacaatt ggccagacat gctaaaagag tattggcatt tgaaattgat caacgattaa
    11101 taccggtatt aaatgataca ctatcacctt atgataatgt gacggtgatt aatgaagata
    11161 ttttaaaagc gaatattaaa gaagctgttg aaaatcattt acaagattgc gaaaaaataa
    11221 tggttgttgc aaacctgccg tactatatta cgacgccaat tttattaaat ttaatgcaac
    11281 aagatatacc aattgatggc tacgtggtga tgatgcaaaa agaagtgggc gaacgcttaa
    11341 atgctgaaat aggttcaaaa gcatatggtt cattatcaat tgtcgtacaa tactatacag
    11401 agactagtaa agtattaacg gtacctaaat ctgtatttat gccaccacct aatgttgatt
    11461 caatagttgt aaaactgatg cagagaactg aaccgttagt aacagtagat aacgaggaag
    11521 cattctttaa gttagcaaaa gcagcatttg cacaaagaag aaagacaatt aacaataact
    11581 atcaaaatta ttttaaagat ggtaaacaac acaaagaagt gattatacaa tggttagaac
    11641 aagcgggtat tgatccaaga cgtcgcggtg aaacgctatc tattcaagat tttgctaaat
    11701 tgtatgaaga aaagaaaaaa ttccctcaat tagaaaatta aatgattgac aaagcaaagc
    11761 actattgtta aaatttaaat tttgtttgac gaaaacgctg caaatatggt attatgtaac
    11821 ttgtagcgag gtggagcaat atgccaaaat caattttgga catcaaaaat tctattgatt
    11881 gtcatgtagg aaatcgtatt gtactgaaag ccaatggagg ccgtaagaaa acaataaaac
    11941 gttctggaat tttaaaagaa acatatccgt cagttttcat tgttgagtta gatcaagaca
    12001 aacacaactt tgagagagta tcttatacat acactgatgt gttaactgaa aatgttcaag
    12061 tttcatttga agaggataat catcacgaat caattgcaca ctaaataaga catatagaga
    12121 tgttagacgt ttcttagtat aagaagtaaa tattatgata a
//
LOCUS       DATTQF010000076        11883 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.076, whole genome shotgun sequence.
ACCESSION   DATTQF010000076 DATTQF010000000
VERSION     DATTQF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.076"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            103..381
                     /locus_tag="VB241_001698"
     CDS             103..381
                     /locus_tag="VB241_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HEP1244990.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            396..764
                     /locus_tag="VB241_001699"
     CDS             396..764
                     /locus_tag="VB241_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1244991.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKQEVKALIDESDALNHSIE
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHDIRKAEEEMIDL
                     EMRRNIEIEMVTKEENKWKQ"
     gene            752..1048
                     /locus_tag="VB241_001700"
     CDS             752..1048
                     /locus_tag="VB241_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEP1244992.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGEVTPQ"
     gene            1381..2487
                     /locus_tag="VB241_001701"
     CDS             1381..2487
                     /locus_tag="VB241_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEP1244993.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNDGQYHISFDSGQSVLTPHEPIVATGFDATKNPI
                     VQQLFATTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            2481..3821
                     /locus_tag="VB241_001702"
     CDS             2481..3821
                     /locus_tag="VB241_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEP1244994.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAVIDQTGFKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            3921..4811
                     /locus_tag="VB241_001703"
     CDS             3921..4811
                     /locus_tag="VB241_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEP1244995.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVVDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVSQMTATSDNKHESLHKTINHQFVQ
                     SPAKCTKAQFIKRLACLPTHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(4958..5788)
                     /locus_tag="VB241_001704"
     CDS             complement(4958..5788)
                     /locus_tag="VB241_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1244996.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELAEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSKFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(5808..6818)
                     /locus_tag="VB241_001705"
     CDS             complement(5808..6818)
                     /locus_tag="VB241_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1244997.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLGVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEAGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            complement(6916..7620)
                     /locus_tag="VB241_001706"
     CDS             complement(6916..7620)
                     /locus_tag="VB241_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP1244998.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSTPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIDA
                     DGPTGTFINGNGEMPW"
     gene            complement(7613..8170)
                     /locus_tag="VB241_001707"
     CDS             complement(7613..8170)
                     /locus_tag="VB241_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1244999.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDHKALL
                     QCIEAGNNKLRFYQSAFYQELFYFYYDLFKSDDDIYTKFKTDMLLQALSTRVFDFQIE
                     HRNISIEAYRNYLLNIYLDEVERND"
     gene            8301..8600
                     /locus_tag="VB241_001708"
     CDS             8301..8600
                     /locus_tag="VB241_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEP1245000.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAGKLNVTTEDVEKAL
                     AMKAPLGIFSHQLQRFIHLVWDVRDVINDNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            8619..9440
                     /locus_tag="VB241_001709"
     CDS             8619..9440
                     /locus_tag="VB241_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEP1245001.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYVFEHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQDYNK"
     gene            9457..9834
                     /locus_tag="VB241_001710"
     CDS             9457..9834
                     /locus_tag="VB241_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1245002.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(9997..10227)
                     /locus_tag="VB241_001711"
     CDS             complement(9997..10227)
                     /locus_tag="VB241_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HEP1245003.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(10447..10947)
                     /locus_tag="VB241_001712"
     CDS             complement(10447..10947)
                     /locus_tag="VB241_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245004.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICPCLLANVRHSSIVCLNNTC
                     RIVDTRCLNTKIGGIGVSETDEPQGFEHTHNRLNINQNSQSLMTFITSKLKSKLKQHI
                     IIARGNKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMAIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(11145..11714)
                     /locus_tag="VB241_001713"
     CDS             complement(11145..11714)
                     /locus_tag="VB241_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1245005.1"
                     /translation="MTESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSGSFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
BASE COUNT         3979 a         1892 c         2155 g         3857 t
ORIGIN      
        1 tataatcaca aaacaatatt taagtataat tgaatgtttg aatatcatat attgatacag
       61 tttctaataa ttttaaaata atttaaatgg gaagaggtgt aaatgatgag tacagttcaa
      121 agtgatattt ttaagactaa tagtgcatca tcatctatta aaagcgctgt tgaaacatgt
      181 aataatgtgt cgaaaccgga taaagatgaa agtacaacag taagtggaaa taataatgct
      241 catagtgtga tagatgattt gattagtaag aatcaatctg ttgctgaagc aatacgaact
      301 gcgagcgata atatacaaaa agttggtgag gcttttgacc aaactgacgt aatgattggt
      361 aatgaaattg gtaaaaatta aaacgtggtg aaatgatgtc gaataaactt gatggaatca
      421 ataagatgat cacagcgaaa cataagcaaa tggatgactt atatgatgaa aagcaagagg
      481 ttaaagcatt gatagatgaa agcgatgcgc ttaatcattc gatagaacaa ttatatcaac
      541 atttaggtga gcgttattat agtagcaata tggctagtcg tatggaacag ttccgcgatg
      601 aatttcattt tgcgaaacga cgttcaacgg aagcgttata cgagcagcaa cagcaaattc
      661 aacatgacat tcgtaaagcg gaagaagaga tgattgactt ggaaatgcga agaaatatcg
      721 aaatcgagat ggtgacaaag gaggaaaata aatggaaaca ataggaagca ttatttattt
      781 aaaagaaggt tcgcaaaagt taatgattat taatagagga ccaattgtag aaattgaaaa
      841 tcaaaagtat atgtttgact attctgcatg taaatatccg attggtgttg tggaagatga
      901 aatttattat tttaacgaag aaaatataga ttcagttatt tttaaaggtt attctgatca
      961 agatgaggtt agatttcaag agttgtttga taatatgaaa caaaatttgg atagtgaaat
     1021 acaacgtgga gaagttacgc cacaataaag aaatacttta tatttattgg agggacgccg
     1081 aaataaattt tgtaaaaata tcatttctgt ctcaccgcca aaatgttatc tccaagtact
     1141 gaggctggat gataccatga aatgtcattc ataatcattt gaactaaccg ttgcatcctt
     1201 aaatttaata aaaaagacac ataccaactt ccgaaatgta gatgaattct ctacaatagc
     1261 ggaagttttt cttttaatat tgaaatttct caaggatagg tctatacttt ataaatcgta
     1321 attattacga tttataatca aaaacaataa cttgaaatag atcattgagg gagtgttaat
     1381 atgcaacatc ataaagtggc tattatcggt gccggtgctg caggtatagg tatggccatt
     1441 accttaaaag atttcggtat aacagatgtc attattttag aaaaaggaac agtaggacat
     1501 tcatttaaac attggccgaa atcgacccgt acgattacgc catcatttac gtctaatgga
     1561 tttggcatgc ctgatatgaa tgcaatttcc atggatactt caccagcatt tacttttaat
     1621 gaagaacata tttccggaga aacatatgct gaatatttac aagtggttgc caaccattac
     1681 gagctgaata tctttgaaaa tacagttgtc acaaatatat ctgtagatga tgcatattat
     1741 acgattgcaa cgacaacaga gacatatcac gcggattata tctttgtcgc aacaggtgat
     1801 tataatttcc ctaaaaagcc atttaaatat ggtattcatt atagtgaaat tgaagacttt
     1861 gataacttta ataaggggca atatgtggtt atcggaggta atgaaagtgg ctttgatgct
     1921 gcatatcaac ttgcaaaaaa tggctctgac atcgcacttt atactagcac aaccggttta
     1981 aatgatccgg atgctgatcc tagtgttaga ttgtcacctt atacacgtca gcgactaggt
     2041 aatgtcatta agcaaggagc tcgcattgaa atgaatgtgc attatacagt taaagatatt
     2101 gattttaacg atggacagta tcatatcagt tttgatagcg gacaaagtgt gcttacgcct
     2161 catgaaccaa tagtagcaac tggctttgat gcaacaaaaa atccaatcgt tcaacaatta
     2221 tttgcgacaa caaatcaaga tattaaatta acaacacatg atgaatcgac acgttatccg
     2281 aatattttta tgattggtgc aacagttgaa aatgataatg ccaaattatg ctatatctat
     2341 aaattcagag cgcgatttgc agtacttgca catcttttaa cacagcggga aggcttacca
     2401 gctaaacaag aagtcattga aaattaccaa aaaaatcaaa tgtatttaga tgattattca
     2461 tgttgtgaag tgtcatgcac atgttagaag tgaaatatga tatgagaacg gggcattata
     2521 cgcccatacc taatgaacct cattatttgg ttattagcca tgcggataaa cttaccgcaa
     2581 cagaaaaagc gaaattaaga ttattaatca taaaacagaa attagatatt tcattggcag
     2641 aaagtgtagt ttcttcgcct atagcgagcg aacatgtgat agaacaattg acactatttc
     2701 aacatgaacg acgacattta agacctaaaa taagtgcgac atttttagcc tggttgttga
     2761 tatttttaat gtttgcattg ccaatcggta tcgcttatca attttcagat tggtttcaaa
     2821 atcagtatgt atcagcatgg atagaatatt taactcaaac aacattgctc aatcacgata
     2881 tattacagca tatattattt ggtgattatg gtgtgctatc acttggaaca tattcgctcg
     2941 tatgggcatt gccggtcgta atattgatta gcttatcaac tgctgtaatt gatcaaacag
     3001 gatttaaatc atggatgata tgggcaattg aaccttcaat gttacggata ggattacaag
     3061 gtaatgatat cgtgccacta ttagaaggat ttggatgtaa tgcagcagct atttcacaag
     3121 cagcacacca atgccatacc tgcacgaaga cacagtgtat gagtttaata agctttggta
     3181 gttcttgtag ttatcaaata ggtgcgacat tatctatttt tagtgtagct ggaaagtcat
     3241 ggctatttat gccgtactta atattagtac ttttaggtgg catcttacat aataggatat
     3301 ggttgaaaaa gaatgatcaa caacttagcg ttccgctacc ttatgatagg caattacata
     3361 tgccaaatat acgtcaaatg ttgctacaaa tgtggcaaaa tatacaaatg tttatcgttc
     3421 aagcgctacc tatttttatc acaatctgtc ttattgttag tattttatca ctaacgccaa
     3481 ttttgaatgt tttatcacaa atatttacac ctatattatc gttattaggc atctcgtcag
     3541 aattgtcacc agggatttta ttttcaatga ttcgaaaaga cggcatgctc ttgtttaatt
     3601 tgcatcaagg cgccttatta caaggaatga cagcaacaca gttactacta cttgtgtttt
     3661 ttagttcaac atttacagcg tgctcggtca caatgacgat gcttttgaaa catttaggtg
     3721 gtcagtcagc actaaaatta attggaaagc aaatggtgac atcattgtct ttagttattg
     3781 gtgtaggcat cattgttaaa atagtaatgc tgattattta aaaaagaaat gaactataac
     3841 tgaatataga gtcatgtcag tcaataggaa agctatcttg gaatatgcta ttcatatgaa
     3901 gtataagagg agagtcgcag atgaaaatag ttattatagg tgggttttta ggtggcggta
     3961 aaacgactgt cttaaatcat ttgctcgctg aatcattaaa ggagtcgctg aaaccggcag
     4021 ttatcatgaa tgaatttggc aaaatgagtg ttgatggtgc cttagtatct gaagacatac
     4081 ctttaagtga actgacagag gggtgtatct gttgtgcaat gaaagcagat gtatcagaac
     4141 agttacatca attatattta aaagagcaac cagacattgt atttattgaa tgtagtggga
     4201 ttgcagaacc agtctctgtc ttagatgctt gtttaacgcc cattttagct ccgtttacaa
     4261 caattacaca tatgattggt gttgtagatg caagcatgta taaacacata aaatcatttc
     4321 caaaagacat ccaaggctta ttttatgagc aattagcata ttgttctgtc ttatttgtta
     4381 ataaaataga ttcagcagat gttgaaacga cgagcaaact attgaaagat ttagaagtta
     4441 ttaacccaga ggccgatata caagttggta tgcatggcag cgtcactttg ccaatatcag
     4501 ttagccaaat gacagcaact tctgacaata aacatgaatc attacacaaa acaataaatc
     4561 atcaatttgt gcaatcacca gccaaatgta ctaaagcaca gtttataaaa cgtttagcat
     4621 gccttccgac tcatatttat aggttgaaag ggtttatgac atttgaagac accgcacata
     4681 cgtatctcat tcaatttaca caaggacaat atgaattaac acctgtagca ttttcaaaaa
     4741 aagtgccaga atatttggtg cttatcggaa agggtatttc aaaagaagac tatcaatgtt
     4801 tggaacagta gtgttttcag tggaagagaa tggttaacat gccttcatgt ataataacga
     4861 gttgatttga acgtttaagc gtaaataaaa ataagcttgg tcagccatca aatataattt
     4921 gaaaactgtc caagctgttt tattagagaa cgatcaatta accccacata tttaataata
     4981 catcagcaaa gccttcaggt ttttgaatat aacctaagtg accgcctgga atatctacaa
     5041 taggtatgcc agtttcttta tttatataaa agttaacatc ttgtgggaag gagcctctag
     5101 aatctgtccc atttagtaag gtgattttat cgctgtattt tgtgaaatca tccaaagtaa
     5161 tatctgaatg cgtatattgt ctaatttcaa attttgacca gaacatcgta cgtttgtact
     5221 gttctatacg tccttcttca gtatcagcag gttgagacat catttttgca tcaattggtg
     5281 cgatatttaa tgtttcacca aatgttttca tacctttttc taagccttct gttaaaattt
     5341 gatgcacaat gtcatcattt ttatctttcc aataagtact gtctggtaaa aatgtattaa
     5401 ttggtggttc atgaaatgca atctttttaa cgacttcagg gtaatctttt aacacatgca
     5461 tcgcaacgat tgaacctgaa cttgaaccta atatatagac aggttcatca cttaatgact
     5521 ttgcaagttc ggcaatgtcc tgtgcgtcgc gtttgacacg ataatcactg tcagggttag
     5581 aagcggaatc agggagtggt tcagctaact cgctttctcc ataatcacga cgatcaacgg
     5641 ctacaacagt aaaatggtct tttaactgtt ctgcaagagg tagaaaaatg tctccggtac
     5701 cgtttgcacc aggaataaag atgagcacgg gtccttgtcc gacttggtgg tagcgtaatt
     5761 tagcgccttg taattctaaa gtttccatat tcaatgacct ccatttgtta attgttagat
     5821 gataaaccta ataatttagc accatttgta taacttattt tctctttttc ttcatctgtt
     5881 aaacccagtt catctaaaaa tacacctaat ttttcaggct caatatatgg ataatcagca
     5941 gcataaagaa ttctatcaat acctgcttct ttcttgacta aatcaaactg tggcttcgtt
     6001 aacataccac tcggtgtgat ataaaaatta tttttaaagt aatagcttac agggtggttc
     6061 aaatgttcag cgaataaagc ttcatccata cgttctaaga agaatgggat aaactcaccc
     6121 caatgtccaa taatcatatt taactttgga taacgatcaa aaatgccgga taatactaga
     6181 tgtattgcat gaataccaac atcaatgtgc caaccataac caaaacaagc aaatgttgcc
     6241 gcggttactt caggataatt tcctttatag tatgattgat aaatgtcact gttaactggc
     6301 gcgggatgta gataaatcgg tacgcctaaa ttttcagctg ttttgaaaat aatgtcatat
     6361 ttgtcttgat caagaaaacc atcttgtgca cgtcccataa tgagcgcacc tttgaatcct
     6421 aaatcattga tgcaacgttc gaattctcgc gctgcggctt caggctcatt gataggtaaa
     6481 gttgcaaagc ctacaaagcg attgggatat tgtgcaatat agtttgccaa ttgatcattt
     6541 gctttttgac ataattcaat ggctttctga ccaactaaat ttgaaggaga cccatttcca
     6601 taagataaga cttgaatttg aacgtcttga ttattcataa attggatacg ttcatcatga
     6661 tgtgataatt cgtcggcatt tgtaaaacct gtcttttttt caaggccttc taacattact
     6721 ttcatcggca cacctttagg atctgccgat atcgcattca tcgtttcttt ttgaatatct
     6781 tcaatgacat aatgttcttc aaacgtaata cttttcattt gattcgcctc catattgttt
     6841 tgcatgtata ttgcatctat tgcagaagca ttttttattt acctctaaat tcaatatttg
     6901 taacataaat ttggattacc aaggcatttc accattccca ttaataaatg tacctgtcgg
     6961 gccatctgca tcaatggttg ctaattgaat gattggcttg atcccttcag aaacatgttt
     7021 tgaattatta ctaaaatcac caactaaatc agtgtttgta gcacctggat cagcagcatt
     7081 gatttgcata tttggtaatc ctttagcgta ttgtaatgtt agcattgtta ctgccgattt
     7141 agacgaacaa taagctaatg aattcacttt agattcagct gtttcggggt ttgtaaccat
     7201 tccaaatgaa cctaaaccac ttgatacgtt gacgacaaca ggttgttcag atttttctaa
     7261 gagagggacg aatgtgttca tcattcgaac gataccaaat acattcgttt gatatacttc
     7321 ttcaacgtca cgaggtgtca atttagaagg tgttgaaaat tgaccagata tacctgcatt
     7381 gttaatgagg atatcaagac gaccttcttt ttcagcaatc atgttataag catttttgac
     7441 agagtagtca cttgtaacat ctaactgtac ataatgaaca cctaattttt gagatgcttg
     7501 ttgtcctctt acgtcattac gagaacctat atacactttg tagcccaatg ctttaagtgc
     7561 ctccgcactt gcatagccta atcctttatt accacctgtg attaacacaa ttttagtcat
     7621 tacgttccac ctcatctaaa taaatgttta ataaataatt tctatacgct tcaattgaaa
     7681 tattgcgatg ttctatttgg aaatcaaata cacgtgttga taatgcttgc aacagcatat
     7741 ctgttttgaa cttcgtgtaa atgtcgtcat cgcttttaaa caagtcataa taaaaataaa
     7801 ataattcttg ataaaatgcg ctttggtaaa aacgtaattt attgttgcct gcttcaatac
     7861 attgcagtag tgccttatgg tcgattttaa attgtaaaag ataatctaac gacacttgca
     7921 taacctcata attagaatga tagtcatctt taatttgctt aaaatgagtg ataaaaatat
     7981 caaggtctct ttgtatgacg tagtagcata aatcgctttt atctttaaaa tgtcgataca
     8041 atgtccccat accgatacct agttctttag caatacgatt catactaatg ttttcaacgc
     8101 cttcttcatc aaaaagtttg tgcgctattt cttcaattcg ttgcctattc tcttttgcat
     8161 cttttcgcat gattccacct acttatagtt ctctaaaatt gacaaacgga taactctccg
     8221 tttattataa aacgtgttaa gaaagttagc aatgaatttg caataactat taaatatcat
     8281 aaaagaaaag agtgttgcgt atgtctttaa ataaagagca aagacgtatt acagctgaag
     8341 aattgcaagc acattttgaa gaatctacct tatcggttca aatgattgca ggaaaactga
     8401 atgttactac agaagatgtg gaaaaagcat tagctatgaa agcgccacta ggcattttta
     8461 gtcatcaatt acaacgattt attcatttag tatgggatgt cagagatgta ataaatgaca
     8521 atattaagga aaatggacaa acaccagaac catatacgta tttaaaaggt gaaaaagagg
     8581 actattggtt tttaagataa aaggggagtg gatctaaaat gaaagatatt ttagtaatag
     8641 gcgcaacagg gaagcaaggt aatgcagttg taaaacagtt gttagaggat ggatggtatg
     8701 taagtgcatt aactagaaat aagaataatc gtaaactttc agacatagga catccgcatt
     8761 tatcaatcgt tgaaggtgat ctgagtgata gtgttagttt gcaatcagcg atgaaaggga
     8821 agtatggttt atacagtatt cagccaatcg ttaaagatga tgttagcgaa gaattaagac
     8881 aaggtatgaa gataattgaa atagctgagc aagaaaatat tcaacatatt gtgtatagca
     8941 ctgcgggagg ggttaatcga aatcgaacag gcccacactt tgaagtacta gcaaaaatag
     9001 aaaatagatt aatggaatct aatataaatg caacagttat caaaccatca ttctttatgg
     9061 ataatttttt acgtattgct aaagtcgaag atgaacgtat tacattacca gaatttatta
     9121 atccgaatat aaaattcaca atgatttctt ctattgatat agctaaaatt gcatcgtatg
     9181 tttttgaaca tccacaaagc tttacgcatc aatcaataga gattggttcc gatgaagtta
     9241 cattaagtga agcagcaaca atttttagtg aagtgacagg taaatcgact gttatagaag
     9301 gagaatttgt tagtggtgtt gcagaaaagc aatggttgga agaaaaaggt tatgaagtag
     9361 actttgaatt aatggctgaa ataaatccaa caagattatc attaagtgat tggcttaaag
     9421 tccaagacta taataagtaa tgaatggggt gcaatcatga ttcaatcaat gtggtttaat
     9481 ttgcatgtgc aagatttaga aaagagcgca cagttttata aagcgttagg atttaaaata
     9541 aatagaaacc cacaaatgtt agataaaatg gtaggcattc aaataggtca aacaaccgta
     9601 attttaatag aaaacaagca ttttcaaaat gtaagtcagc aaagccttaa cactgaacca
     9661 aatgaagtga tgatttcttt aggtgtgaat acaaatgaag aagttgacca gttggtgaat
     9721 aaagtgaaag aagcgggtgg cacagtcgtt caagaaccaa cagtaagcca agggttttat
     9781 ggtgcaatgt tcaaagatct tgatggacac cattttaatt ttttagtctg ctaaaatgaa
     9841 attctcaaaa gaataaataa aaagcccata cagagatgaa gcgtaatgtg cgcatgtcgt
     9901 aagagacatg gtgcgacaca ttagtgtctt caatcagtat gggctttgtt tatgaggtca
     9961 gttggcacct taagtgcaat acctcgtgag caaatattaa ttttgaatat aaccgtattg
    10021 ttggcgaaga tcataaactt tatctggtgt gacatcctga ccgattggat caaccacttt
    10081 tattgtggaa aatgttgtta atacttgtcc ccggatcatg cttaagtagt cttggcgcaa
    10141 tgcatgttgt tcttgttttt cagctacagt aagtggttgt actttttctt tatttgcaag
    10201 ttcattaatt ctatctaaaa ttttcatatt gtcatatcct ccttagaatc aagtgttgat
    10261 atattttgag attatttcta tcaggtaatt tatcaacaca ttcagtaatg tttttagttt
    10321 ctgggtcttt tgaccagcag atttttggaa ttaaaataag gggtttgaga cacgatttta
    10381 atagtagtac gacggcaaag tgatgttatt gaagtgccgt ttgatataca tgtctgcaat
    10441 gttgttttat aagacaggat gacgtaactg agcaaagaaa tgagcattgt cgaatgacat
    10501 cgtgtgtctt attaaaattg caatggccat cgcataggta tattcacagt cgattttctt
    10561 gatttctaca ttatcataat acgtagtgta aaagttatac gacagtcgat agtcgattcg
    10621 cttattacca cgagcaatta ttatgtgctg cttcaacttc gacttcaatt tacttgtaat
    10681 gaatgtcatt agactctgac tattctgatt aatatttaat ctgttatgcg tgtgttcaaa
    10741 cccttgaggt tcatctgtct cgctcacgcc aatccctcct atcttagtat ttaaacaacg
    10801 cgtatcaact atgcgacatg tgttgtttag acatactata ctactatgtc gtacgtttgc
    10861 caataaacac ggacatatca tttgttgcct aaacaacata ttgcctgaag tgttgtttaa
    10921 gtacgcaaaa acaaaaatgc ttgtcactaa aatctatatg ttttaatgac aagcttattt
    10981 tcattcagtt aacgactgcc aatatggctt tgtagagtat aggacgttga tttatgtcct
    11041 agacacggct aatcaatcgt aatacgttaa attttattgt gtgtacaagt atgaacctaa
    11101 gtcacatagt gatttcaaga tgcatgacta aataccaagt tttattaaaa atgtgctata
    11161 tgatgaatta aaaatggaag gttgcgatcg ataaacacat cggctgtttc ttgactcgtg
    11221 ttaaaccaat gtaatgctaa gcctgatata ccagctgcta aaaaactccc actatttaca
    11281 atgatatctc gttgatgctc tgggtagctg ttttctaaca taatcgcaaa aatatcttct
    11341 aattcattat taatacgttg atgtgctttg ttgcaaaatg cttcactatt taattcgcaa
    11401 gactttgcag catctttgat ataactcgca attgaaatga aaatattacg cagtaccttt
    11461 tcattaatga cattagaaat gctcaagtta tcattcaagt cttttaaaat ggttacagac
    11521 aatgtgtagt ctaggaggtc ttctttatca gtgaaatgag cgtaaaatgt tgcacgattt
    11581 aacgtagcaa tatcagtgat atctttaact gtaatttgag acaatttctt ttctctagaa
    11641 attttaagaa aagcatcgac aagcaattgt ttggttctaa cgattcttgg atctatggat
    11701 ttactttccg tcatttgttc actacctaac atctgttgtt ttaacactta ttatcagtat
    11761 cttctattat gttgtcaatt gtgttgatga tatgtaagtg ctgatacgct cctaacaaat
    11821 aatgtatctt tttggtaagc atttgaatgt ctttcttcta ttatattaat ggattttaaa
    11881 aat
//
LOCUS       DATTQF010000077        11828 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.077, whole genome shotgun sequence.
ACCESSION   DATTQF010000077 DATTQF010000000
VERSION     DATTQF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.077"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..587
                     /locus_tag="VB241_001714"
     CDS             <1..587
                     /locus_tag="VB241_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP1245006.1"
                     /translation="FKYFEHNVAAHSFKVTKIAQYLATVEEYHGRKINWKSLYEKALN
                     HDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVETFIEEEIPLQYRDVYKQRLQEGK
                     DDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFEIYEMSLETIIQFDHLASVQDFI
                     NNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            584..820
                     /locus_tag="VB241_001715"
     CDS             584..820
                     /locus_tag="VB241_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HEP1245007.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1089..3074
                     /gene="uvrB"
                     /locus_tag="VB241_001716"
     CDS             1089..3074
                     /gene="uvrB"
                     /locus_tag="VB241_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEP1245008.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3082..5928
                     /gene="uvrA"
                     /locus_tag="VB241_001717"
     CDS             3082..5928
                     /gene="uvrA"
                     /locus_tag="VB241_001717"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP1245009.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNINLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6539..7471
                     /locus_tag="VB241_001718"
     CDS             6539..7471
                     /locus_tag="VB241_001718"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP1245010.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7477..8319
                     /locus_tag="VB241_001719"
     CDS             7477..8319
                     /locus_tag="VB241_001719"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP1245011.1"
                     /translation="MGGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALV
                     KAGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLI
                     GGFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQL
                     HLPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIG
                     RFFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPT
                     RKVK"
     gene            8327..8812
                     /locus_tag="VB241_001720"
     CDS             8327..8812
                     /locus_tag="VB241_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP1245012.1"
                     /translation="MRKFLSKEYHRTNPLWHVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQILNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            8820..10259
                     /locus_tag="VB241_001721"
     CDS             8820..10259
                     /locus_tag="VB241_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP1245013.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLKEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            10326..11261
                     /gene="trxB"
                     /locus_tag="VB241_001722"
     CDS             10326..11261
                     /gene="trxB"
                     /locus_tag="VB241_001722"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP1245014.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSAPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         4227 a         1626 c         2348 g         3627 t
ORIGIN      
        1 aatttaaata ttttgaacac aatgttgcag cgcactcctt taaagtaact aaaattgctc
       61 aatatctagc aacagttgaa gaatatcatg gacgaaagat taattggaaa agcttatatg
      121 aaaaagcatt aaatcatgat ttcgctgaag tgtttactgg tgatataaaa acacctgtta
      181 aatatgcgag tagtgaatta aaaaaattat tttcgcaagt tgaagaagaa atggtagaga
      241 cctttattga agaagaaatt ccattacaat atagagatgt ttataagcaa cgactgcaag
      301 aaggtaaaga tgattcatta gaaggccaaa tactttcagt tgctgataaa attgatttgc
      361 tttatgaaac atttggagaa atacaaaaac gtaatcccga agaattattt ttcgaaattt
      421 atgaaatgag tctagaaaca attattcaat ttgaccattt agcatctgta caagatttta
      481 ttaataatat cattccagaa atgttgactg aaaactttat acctagaaca gaattaagag
      541 aaacaactat gaacatttta aataaaagaa aagaggaaaa tgaatgatat ggtattttag
      601 cgcagcattc tttccatgtg tcttggtagt attgtttagt gtaataacaa gaagtaaatg
      661 ggtcggtact attctgacat taattttaat tggtgcctca atctataaag agtatttcca
      721 taacgagtgg attattttta ttgatgtggt gtcattatta gctggttatt taattataga
      781 tcaactcgaa tttcataaac atcaagatga agatcgctaa gattaacttt aaaataatgt
      841 ttcaaacaaa tttgttgaaa caaaatgatg attaatataa tgtgtattta catactaaaa
      901 ataacaagat aaacgatttg atttaaggca agcatagtta gcacaactat gtttgttttt
      961 ctttgttcga catttttacg aacaaacgtt tgctttttgt gtgactactt tgctaaaata
     1021 tgtaatgaga aagcgaagag ttgagcggaa atataaataa tacgttgaaa gaggaggcat
     1081 atgtgactat ggttgaacat tatcctttta aaatacattc tgattttgag cctcaaggtg
     1141 accaaccgca agcaattaag gaaatcgtgg aaggtattaa agcggggaaa agacatcaaa
     1201 ctttattagg tgcaactggc acagggaaaa catttacgat gagtaatgtt attaaagaag
     1261 ttgggaaacc aacgttaatt atcgcgcata acaaaacatt agcaggacaa ttatatagtg
     1321 agtttaaaga attttttcct gaaaacaggg tggaatactt tgtaagttac tatgattatt
     1381 atcaacctga ggcatacgta ccgtctactg acacttttat tgaaaaagat gcctcaatca
     1441 atgatgaaat tgatcaacta cgacattctg ctacaagtgc attatttgaa cgcgatgatg
     1501 taattattat tgctagtgta agttgtatat atggtttagg taatcctgaa gaatataaag
     1561 atttagtagt aagtgttcga gttggtatgg aaatggatag aagtgaatta cttagaaaac
     1621 ttgtagatgt gcaatataca cgaaatgaca tcgatttcca acgaggaacg tttcgagtgc
     1681 gtggtgatgt tgttgaaata ttcccagcct ctaaagaaga actttgtata agggttgagt
     1741 ttttcggcga tgagattgac cgtattcgag aagttaacta cctaactggt gaagtgttga
     1801 aagaaagaga acattttgcg atattcccag cttctcactt cgtaacacgt gaagaaaagt
     1861 tgaaagttgc gattgaacgt attgaaaaag aattggaaga acgattgaaa gaattacgag
     1921 atgagaataa attactagaa gcgcaaaggt tagaacagcg taccaactat gatttagaaa
     1981 tgatgcgaga gatgggattc tgttcaggaa ttgaaaacta ttccgtacat ttaactttgc
     2041 gaccactggg ttcgacacca tatactttat tggattactt tggcgatgat tggttagtaa
     2101 tgattgatga atcacatgtg acattaccgc aagttcgagg catgtataac ggagacagag
     2161 cgcgtaaaca agttttggtg gatcatggat ttagattacc gagtgcatta gataaccgtc
     2221 ctcttaaatt tgaagaattt gaagaaaaga caaaacaact tgtgtatgta tctgcaacgc
     2281 ctggaccata cgaaattgaa catacggata agatggttga acaaattatt cgtcctactg
     2341 gtttactgga tcctaagatt gaggttagac ctactgaaaa tcaaattgac gatttattaa
     2401 gtgaaattca aacaagggtt gagcgtaatg aacgcgtact tgttacaacg ctcactaaaa
     2461 agatgagtga agatttaacc acatacatga aagaagcggg tattaaagtt aattatctgc
     2521 attcagaaat caagacatta gaacgaatcg aaataattag agacttacga atgggtacat
     2581 atgatgttat cgtaggtatt aatttattaa gagagggtat tgatatacca gaagtttctc
     2641 tagttgtcat attagatgca gataaagaag gatttttacg ttctaaccgc tcattaattc
     2701 aaacaatagg tagagctgcg cgtaacgata aaggtgaagt cattatgtat gccgataaaa
     2761 tgactgattc gatgaagtat gcaattgatg agacacaacg tcgtcgagaa atacagatga
     2821 aacataatga aaaacatggt attacaccta aaacaattaa taaaaaaata catgatttaa
     2881 ttagtgctac tgttgaaaat gacgaaaata atgacaaagc acaaactgtg atacctaaga
     2941 agatgacgaa aaaagaacgt caaaagacaa tcgacaatat agaaaaagaa atgaaacaag
     3001 cagcgaaaga tttagatttc gagaaagcta cagaattaag agatatgtta tttgaattaa
     3061 aagcagaagg gtgacaagta aatgaaagaa ccatccatag tagtaaaagg tgctcgtgcg
     3121 cataacttga aagatattga tatcgaacta cctaaaaata aattaattgt tatgacagga
     3181 ttatctgggt caggtaaatc gtcattagca ttcgatacta tatatgctga aggacaacga
     3241 cgttatgttg aatcattaag tgcctatgcg cgtcaatttt tagggcaaat ggacaaacca
     3301 gatgttgata ctattgaagg attatcgcca gcaatttcaa tagatcaaaa aacaacaagt
     3361 aaaaatccaa ggtcaactgt tgcaacagta acagaaatat atgattatat acgtttgtta
     3421 tatgcacgtg ttggtaaacc ttactgtcca aatcacaata tagaaattga atcgcaaaca
     3481 gtacaacaaa tggttgaccg cattatggaa ttagaggcac gtacaaagat tcaattatta
     3541 gcacctgtca tcgctcatcg taaaggtagt catgaaaagc taatcgaaga tattggtaaa
     3601 aaaggttatg tacgtttaag aatcgatggc gaaattgttg atgtaaatga tgtacctaca
     3661 ttagataaga acaagaatca tacaatagaa gttgttgtag accgattagt tgttaaagat
     3721 ggaattgaaa cacgactagc tgactctata gaaactgcgt tagagctttc agaaggacaa
     3781 ttaacagtcg atgtcattga cggggaagac cttaagtttt cagaaagcca cgcttgtcct
     3841 atatgtggat tttcaatcgg agaattagaa ccaagaatgt ttagctttaa cagtcctttt
     3901 ggtgcttgtc caacatgtga tggcttaggt caaaagttaa cagtcgatgt agacttggtt
     3961 gttcccgaca aagataagac gctaaacgaa ggtgcaatag aaccatggat accgacgagt
     4021 tctgattttt atccaacatt gttaaaacgt gtttgtgaag tttataaaat caatatggat
     4081 aaacctttta aaaagttaac agaacgtcaa cgtgatattt tattgtatgg ttcgggtgac
     4141 aaagaaattg aatttacatt tacacaacgt cagggtggta ctagaaaacg aacaatggtt
     4201 ttcgagggtg tagttcctaa tataagtaga cgattccatg aatctccttc agaatataca
     4261 cgtgaaatga tgagtaaata tatgactgaa ctaccttgcg aaacttgtca tggaaagcga
     4321 ttgagtcgtg aagcgttatc tgtttatgta ggtggtttaa atattggtga agtagtcgaa
     4381 tactcaatta gtcaagcgct gaactattat aaaaacatta atttgtcaga acaagatcaa
     4441 gcgattgcaa atcaaatatt gaaagaaatt atttcccgac tcactttttt aaataatgtg
     4501 ggacttgaat atttaacgct aaacagagct tcaggtacac tttcaggtgg tgaagcacaa
     4561 cgtattcgat tggcaacgca aattgggtcg cgtttgactg gtgtcttata tgtattagat
     4621 gagccatcaa ttggactgca tcaaagagat aatgatcgat taattaatac acttaaagaa
     4681 atgagagatt taggaaatac tttaattgta gttgaacacg atgatgatac aatgcgtgcg
     4741 gctgattact tagtggacat agggcctggt gctggtgagc atggaggaca gattgtgtct
     4801 agtggtacac ctcaaaaggt aatgaaagat aaaaaatctt taacaggaca atacttgagt
     4861 ggtaagaaac gtattgaagt acctgaatat cgcagaccgg cttcagatcg taaaatttct
     4921 atacgtggag ctagaagtaa caaccttaaa ggggttgatg tggacatacc actttcaatc
     4981 atgacggtcg ttacaggtgt atcaggttct ggtaaaagct cattagtaaa tgaagtatta
     5041 tacaaatcat tagctcaaaa aatcaataaa tctaaagtaa agccaggatt gtacgataaa
     5101 attgaaggta ttgatcaact tgataaaatt attgatattg atcaatcacc gataggtaga
     5161 acgccacgct ctaatccagc aacatatact ggcgtgtttg atgatatacg tgatgtgttt
     5221 gcgcaaacaa atgaagctaa aattcgagga tatcaaaaag gtcgttttag ttttaatgta
     5281 aaaggtggac gctgtgaagc ttgtaaaggt gacggtatta ttaaaattga aatgcatttt
     5341 ttacctgatg tttatgttcc ttgtgaagtg tgtgatggta aacgatataa tcgcgagaca
     5401 ctagaggtta cttacaaagg taaaaatatt gctgacattt tagaaatgac tgttgaagaa
     5461 gcaacacaat tttttgaaaa tattcctaag attaagcgca agctacaaac actagttgat
     5521 gttggtcttg gatacgtcac attaggtcaa caagctacaa cattatcagg tggtgaggct
     5581 caacgtgtga aacttgcatc tgaacttcat aaacgttcaa ctggtaaatc tatttatatc
     5641 ctagacgaac cgacaacagg gttacatgtt gacgatatta gtagattatt aaaagtatta
     5701 aaccgattag ttgaaaatgg tgacactgtt gtaattattg aacataacct agatgttatc
     5761 aaaacagcag actatattat agacttaggt cctgaaggtg gtagtggcgg tggtactatt
     5821 gttgcgactg gaacacccga agatattgct cagacaaagt catcatatac aggaaagtat
     5881 ttaaaagaag tacttgaacg agataaacaa aatactgaag ataaataaga ttaaaagaag
     5941 tgaaggatgt tgtaaattta tccttcactt ctttttatta atttagtaat gaatagtaga
     6001 aagaaaagat gcgtaaaaag aattatgtta agataaggtc aatctagagt agctaaacat
     6061 aaatcgaaac tgggagcggg acagaaatga taaagaatca ctaatgattt attatgtagt
     6121 ggttctttgt cattagccac agctattgtg tacttaaaaa taggaatgca tgagtgcaac
     6181 tcatgcataa gaaatactaa tttctaaaga aaaagtatct ctttatgttg ggggcccgcc
     6241 ccaacttgca ttgtctgtcg aaattgagaa tccaatttct ctgtgttggg gccccgccaa
     6301 ctaattcgaa tttatcattt tagagcttag gtcattgatt tatggctcgg acttttatgg
     6361 cgatatgaaa catgtaaatt aagcaagcaa taaattaatg attgatattg acttgtaaaa
     6421 taataacaat aatgaacaat taatatttat tttagctttt caatgtagat tggtgttata
     6481 tttttgatat gataagaaga gatgtaagag tagggataaa tacaattgag gtgaacccat
     6541 gttaacgaca gaaaaactag ttgaaacatt aaagttagat ttaatcgctg gtgaagaagg
     6601 gctatcgaag ccaattaaaa atgctgatat atcaagaccg ggcttagaga tggcaggtta
     6661 tttttcacat tatgcgtcag atagaataca actattagga acaacggaac tatcgtttta
     6721 caatttatta ccagataagg atcgcgcagg tcgtatgcgt aaactatgca gaccagaaac
     6781 gcctgcaatt attgtgacac gtggattgca gccaccagaa gaattagttg aagctgcaaa
     6841 agaattaaat acgccactta tagttgctaa agatgccact acaagtttaa tgagtcgctt
     6901 aacgacgttt ttagagcatg cacttgcaaa gacgacatct ttacatggag ttttagtaga
     6961 tgtttacggt gttggtgtac taattaccgg tgattcagga ataggtaaaa gtgagactgc
     7021 gttagaatta gtgaagcgtg ggcatagatt agtagcagat gataatgtag aaatacgtca
     7081 aattaataaa gatgaactaa tagggaaacc accaaagtta atagaacatc tattagaaat
     7141 acgtggacta ggtattatca atgttatgac tttatttggc gcgggttcaa tattaactga
     7201 aaaacgaatt agattaaata ttaatttgga aaactggaac aagcaaaagt tatatgaccg
     7261 cgtaggtctt aatgaagaga cgttaagtat tttagatact gaaataacta aaaaaacaat
     7321 acctgtaaga cctggtagaa atgttgcggt aattattgag gtcgctgcaa tgaactatcg
     7381 attaaatatc atgggcatta acactgccga agaatttagt gaaagattaa atgaagaaat
     7441 tatcaagaac agtcataaga gtgaggagta ggttgaatgg gtggtattgt atttaactat
     7501 atagatcctg tggcatttaa cttaggacca ctaagtgtac gatggtatgg aattatcatt
     7561 gctgtcggaa tactacttgg ttactttgtt gcacaacgtg cactagttaa agcaggatta
     7621 cataaagata ctttagtaga tattattttt tatagtgcac tatttggatt tatcgcggca
     7681 cgaatctatt ttgtaatttt ccaatggcca tattacgcgg aaaatccagg tgaaattatt
     7741 aaaatatggc atggtggtat agcaatacat ggcggtttaa taggtggctt tattgctggt
     7801 gttattgtat gtaaagtgaa aaatttaaac ccatttcaaa ttggtgatat cgttgcgcca
     7861 agtataattt tagcgcaagg aattggacgc tggggtaact ttatgaatca cgaggcacat
     7921 ggtggacctg tgtcacgcgc ttttttagaa caattacatt tgcctaattt tataatagaa
     7981 aatatgtata ttaacggcca atattatcat ccaacattct tatatgaatc catttgggat
     8041 gtcgctggat ttattatctt agttaatatt cgtaaacatt taaaattagg agaaacattc
     8101 tttttatatt taacttggta ttcaattggt cgattcttta tagaaggatt acgtacagat
     8161 agcttaatgc tcacaagtaa tattagagtt gcacaattag tatcaattct tttaatttta
     8221 ataagtataa gtttaattgt atatagaagg attaagtata atccaccgtt gtatagcaaa
     8281 gttggggcgc ttccatggcc aacaagaaaa gtgaagtagt gatattttga gaaaattttt
     8341 atcaaaagaa tatcatcgta caaacccttt atggcatgta taccgtcttg ttaaattttc
     8401 gaaagttttt aagaatgtaa tcatcattga attttcgaaa tttattccaa gtatggtact
     8461 gaaaagacat atatataaac aaattttaaa tattaatatc ggtaatcaat cgtcgatagc
     8521 ttataaagta atgttagata ttttttatcc agaactgatt acgattggta gtaacagtgt
     8581 tattggttac aatgtaacaa ttttgacgca tgaagcatta gttgatgaat ttcgttatgg
     8641 accagtgacg ataggatcta acactttgat tggtgcaaat gctaccattt tacccggtat
     8701 aacgattggt gacaatgtaa aagttgcagc tggtacggtt gtttcaaaag atataccgga
     8761 taatggattt gcatatggca accctatgta tataaaaatg attaggaggt gacaatttta
     8821 tggcgcaaaa gaataataat gtaattccaa tgacttttga tgatgcattt tatcgtaaaa
     8881 tggctaatca gaagtttaaa caaagagaat ataaacgagc tgctgaatac tttgaaaaag
     8941 tgttagaatt gtcacctgat gatctggaaa ttcaaattga ttatgcacaa tgtctagtgc
     9001 aacttggtat tgctaaaaaa gcagaacatt tattttatga caatattatt tataataggc
     9061 atctagaaga tagcttttat gaattgagtc agctcaacat tgaagttaac gaaccaaaca
     9121 aggcattctt gtttggtatt aattatgtta ttgttagcga cgaccaagat tatagagatg
     9181 aattagatca aatgtttgat gtgaaatatc aaagtgaaga acaaattgaa cttgaagctc
     9241 aattgtttgt agttcaaata ctattccaat atcttttttc tcaaggtcga ttaaaagatg
     9301 caaagaatta tgtcttacat caaccacaag aagttcaaga tcatcgtgtc gtacgtaatt
     9361 tactggcaat gtgttattta tatctcggcg aatatgatac ggctaaagca ttgtacgaag
     9421 cactattaaa agaggatagt acagatatat atgcattatg ccattatact ttgctacttt
     9481 ataacactaa ggaaaatgaa caataccaaa agtatttaaa aatattaaat aaagttgtac
     9541 ctatgaatga cgatgaaagt tttaaattag gtattgtttt aagttattta aagcagtatc
     9601 gtgcttcaca acaattgttg taccctttat ataaaaaagg gaaattttta tcaattcaaa
     9661 tgtacaatgc tttagcatat aattattatt atttaggtga agaagacgaa agtcattact
     9721 actgggataa attgaagcaa atttctaaag tggaaattgg acatgctcct tgggtaattg
     9781 aaaatagcaa agaagttttt gaccaacata ttttgccatt acttcaaagt gatgacagtc
     9841 attatcgttt atatggtatt tttttattgg atcaattaaa tggtaaagaa attgtgatga
     9901 cggaaagtat ttggcaggtt ctggaaaatc taaataatta tgagaaattg tatttaacgt
     9961 atttagttca aggtttaacg ctcaataaat tagacttcat tcatcgcggc ttgttaacgc
    10021 tttaccataa tgaattattt gtaagtgaaa atgatttaat ggttgcatgg attaatcaag
    10081 gtgaactcat aattgctgaa aaagtagatt taactgatgt tgagccatat atcggtgcgt
    10141 ttatttattt gtattttaaa aatcaacctc gaaacgttac aaagaagcaa attacaacat
    10201 ggttaggcat aacacaatat aaactgaaca aaatgattga atttctcttg agcatataga
    10261 tttatgaaaa gttagattta ttatataatg cgcataatga ttaataatga ggaggcgtta
    10321 ataaaatgac tgaaatagat tttgatatag caattatcgg tgcaggtcca gctggtatga
    10381 ctgctgcagt atacgcatca cgtgctaatt taaaaacagt tatgattgaa agaggtattc
    10441 caggcggtca aatggctaat acagaagaag tagagaactt ccctggtttc gaaatgatta
    10501 caggtccaga tttatctaca aaaatgtttg aacacgctaa aaagtttggt gcagtttatc
    10561 aatatggaga tattaaatct gtagaagata aaggcgaata taaagtgatt aactttggta
    10621 acaaagaatt aacagctaaa gcggtcatta ttgctacagg tgcagaatac aagaaaattg
    10681 gtgttccggg tgaacaagaa cttggtggac gcggtgtaag ttattgtgca gtatgtgatg
    10741 gtgcattctt taaaaataaa cgcctattcg ttatcggtgg tggtgactca gcagtagaag
    10801 agggaacatt cttaactaaa tttgctgaca aagtaacaat cgttcaccgt cgtgatgagt
    10861 tacgtgcaca acgtatttta caagatagag cattcaaaaa tgataaaata gactttattt
    10921 ggagccatac tttgaaatca attaatgaaa aagacggcaa agtgggttct gtgacattaa
    10981 cgtctacaaa agatggttca gaagaaacac acgaggctga tggtgtattc atctatattg
    11041 gtatgaaacc attaacagca ccatttaaag acttaggtat tacaaatgat gttggttata
    11101 ttgtgacaaa agatgatatg acaacatcag caccaggtat ttttgcagca ggagatgttc
    11161 gcgacaaagg tttacgccaa attgtcactg ctactggcga tggtagtatt gcagcacaaa
    11221 gtgcagcgga atatattgaa catttaaacg atcaagctta aatcgaagtc gaattaagat
    11281 gttgcgttgt aaattatttg gatatttatt ttaatagtga catcacattg ttaaaataat
    11341 gtcttacttt taaattaaag caaattatat agaaaactag aacttagtac gtatcatttg
    11401 tgcgtttcaa tgagttctag tttttttata tgttatatta aacttataac tttatgggag
    11461 tgggacagaa atgataaaga gccactaatg atttattatg tagtggttct tacacattag
    11521 ccacagctaa tgtgtactta aaaataggaa tacatgagta aaactcatgc ataagaaata
    11581 ctaatttcta tagaaaaagt atttctttat cgttgtcccg ccccaacttg cacattattg
    11641 taagctggcg gaaagtcagc ttctgtgttg gggcccaccc cccaactcgc attgcctgta
    11701 gaatttcttt tcgaaattct ctgtgttggg gccccacccc aacttgcaca ttattgtaag
    11761 ctgactttac gtcagcttct ttgttggggc ccctgactat aattgaaaaa agcttgttgc
    11821 aagcgcat
//
LOCUS       DATTQF010000078        11765 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.078, whole genome shotgun sequence.
ACCESSION   DATTQF010000078 DATTQF010000000
VERSION     DATTQF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.078"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            407..1102
                     /locus_tag="VB241_001723"
     CDS             407..1102
                     /locus_tag="VB241_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1245015.1"
                     /translation="MDVLTIEHLTKKIGNKTILDDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLDIIEREDQA"
     gene            1104..1874
                     /locus_tag="VB241_001724"
     CDS             1104..1874
                     /locus_tag="VB241_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245016.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQSYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            2379..4343
                     /locus_tag="VB241_001725"
     CDS             2379..4343
                     /locus_tag="VB241_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEP1245017.1"
                     /translation="MTQITERELKKKYLDLLSQHFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKDKLSTKELNDLTALVYYPEDKLQL
                     IKCDFQNYGQLNVWYITTIEHLIQLIKYCSSKYTRSKLRRALPEQYVFIVEELLYKNN
                     EFKNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIVEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPVIKRRP
                     NFEMDERLVLEKVNYDTNEITVYGKTYPLKDTCFQTVNRDNPAKLLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPCNGNLLIHGCIPVDENGEMESFEIDGQTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRIVDKELQ
                     RKKIRNTNKGKELQAQIDILKMLMHDRYLD"
     gene            4687..5766
                     /locus_tag="VB241_001726"
     CDS             4687..5766
                     /locus_tag="VB241_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245018.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVVFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHFDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5871..6464)
                     /locus_tag="VB241_001727"
     CDS             complement(5871..6464)
                     /locus_tag="VB241_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1245019.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPIEVRSTKKLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAQVEEVWVNGHEITETGLTAGQQILEK"
     gene            complement(6486..7454)
                     /locus_tag="VB241_001728"
     CDS             complement(6486..7454)
                     /locus_tag="VB241_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEP1245020.1"
                     /translation="MTNNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNIALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHD"
     gene            complement(7532..7966)
                     /locus_tag="VB241_001729"
     CDS             complement(7532..7966)
                     /locus_tag="VB241_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1245021.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            8192..8476
                     /locus_tag="VB241_001730"
     CDS             8192..8476
                     /locus_tag="VB241_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1245022.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8651..9457
                     /locus_tag="VB241_001731"
     CDS             8651..9457
                     /locus_tag="VB241_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1245023.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9578..10249)
                     /locus_tag="VB241_001732"
     CDS             complement(9578..10249)
                     /locus_tag="VB241_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP1245024.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10519..11511
                     /locus_tag="VB241_001733"
     CDS             10519..11511
                     /locus_tag="VB241_001733"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP1245025.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     TMFDHVKKGAILVNAARGAVINTPDLIDAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
BASE COUNT         3823 a         2083 c         1864 g         3995 t
ORIGIN      
        1 tggcgaattg ttgcacttaa tttgacaacc taaaacttaa gaaaatattc agtcacacaa
       61 cctatcttac ttattactta tttaattccg tatacccatc tcacaaatcc atctataata
      121 caaactatca ttttatataa gcagaattta ccctgtcttc cacacaaact gttttcgtca
      181 ctttaatatg tcttctattc aaaaaagtca ctcataacaa attgcgcatt ttaataaatt
      241 tagcaatgcc atttgcatgc tgcaaaaata ctcatattaa tatttatatt ttatattatt
      301 taagtttttt aaatccatat ttttataaca cttgctatgt gataaaatta attttatata
      361 tataatctta agattcagat tatttaatag caaaggagat agtgatatgg atgttttaac
      421 aatagaacat ttaacaaaga agataggtaa taaaacgatt ctcgacgatg tatcatttaa
      481 gctgaaacgc ggacaaatag ttggtcttgt tggagcgaat ggtgcaggta aaacaacttt
      541 aatgaaagtt atattaggtt actctagttt ccaaagcggg aattttaatg ttattaacag
      601 caaggatagc aaaagcaata tcggtgcatt gattgaaaat ccaggaatat atccttttat
      661 gtctggatat gaaaacttga agttattgaa tgaatcaaaa aacactcaag atatcgataa
      721 aattgtctca caacttcata tggatgaata cattcataaa aaagctaaaa cgtattctct
      781 tggtatgaaa caaaaattag gaattgctat agcattttta aatgaacctc aatttattat
      841 cttagatgaa ccaatgaatg gcttagatcc aaaagctgtg cgagatgtac gtgaattgat
      901 tgtccaaaaa gcgcaagaag gtgtcacttt cttaatttcg agtcatattt taagtgaatt
      961 agttaaaatc acaaactcta tccttattat taacaaaggt aaaattgtta cagaaacatc
     1021 ggaagaagaa cttaaacaat ttaaagataa tgatttagaa aatgtattac tagacatcat
     1081 agaaagggag gatcaagcat aaaatgggaa ctttaattaa acaagaatgt ttcaaattat
     1141 ttaaaaagaa atcaactttt atcgcaccta ttgtctttat tctactaatg gttgctcaaa
     1201 gttatattgc tacaaaatac aatgaaattt ttacaccaca ggaatctttt acatctgctt
     1261 ataatggttt ttcatggttt gcatttttat taattattca agcaagtaca atcatttcaa
     1321 tggaatttca ttacggtacg attaaaaatt tactctatcg tgaatattca agaacaacta
     1381 tgattgttag caaaatcatc acattattta ttatttcttt aatttatttt gttattacaa
     1441 ttattgcttc aattgttatt gggtctttat tctttaatga tttaaatata tttgaaagta
     1501 gcggtaatca attatcttta ttgaatcaat tattattagt tagtttaggt acatttgttg
     1561 gtgtttggtt agttttaagc ttaacgttgc tattatcatc tgcaactaat tcaacgggtg
     1621 tagccattgc tgtaggtatt gttttttatt ttgcaagttc tattttagca gttattcaaa
     1681 cggcactttt agaaaaaata gactggctaa agtggaatcc tattaatatg atgaatatta
     1741 tgcttcaaac agttgaaaaa ggctttagta agtcgacgaa attagaactt catgaattgt
     1801 ttattggtaa tattgcttat atttctattt tcttaatact tgtagtattt attttcaaga
     1861 agaaaaatat ttagtaactt aaagtattag atgaataaat acacacatat ttcatcgtaa
     1921 ttcaaaatta ttttcaaatc ccttcaccca aataatggtg cagggatttt tgatatgtca
     1981 gcatgccact cattctatct ttaatatatt tcaaaatccc cccacttgta atgtacaaaa
     2041 tatgctcact taaacgataa tcgtatctct gccaagctat ttcaactctg ctttaatata
     2101 aaaattattc tcccggatgg gatgcttata tctctatcat gatatttcaa cgccgcttca
     2161 acaactttat cacttcatat ttatgcgggt acacttcaac gctactaata tgaatgatac
     2221 aaccacagat tctcaacact aaaattcaca atatgtttac tctattttta tcaattcaag
     2281 catacttatt gaaaaatgtt aacgttttct tgataatcat tgtaagcgta ttaattttac
     2341 aaaccaatga ttgaaatata ctacaggagt gacacacaat gactcaaatt actgaaagag
     2401 aattaaaaaa gaaatatttg gatttattat ctcaacattt cgatactcca gaaaagcttg
     2461 cgactgaaat tattaattta gaatctattt tagaattgcc taaaggtacg gagcatttcg
     2521 tcagcgattt acatggtgaa tatgaagctt tccaacacgt attacgcaac ggttctggga
     2581 acgtgcgagc gaaaattaat gatattttca aagacaagtt atcaacgaaa gaacttaacg
     2641 atttgactgc ccttgtttac tatccagaag acaaattgca acttattaaa tgtgattttc
     2701 aaaattatgg acaattaaat gtttggtata ttacgacgat tgaacacctc attcagctaa
     2761 ttaaatattg ttcttcaaaa tatacgcgtt caaaacttcg acgtgccttg ccggagcaat
     2821 acgttttcat cgtagaagag ttattatata agaacaacga atttaaaaat aaaaaatctt
     2881 actacgaaac acttgtaaac caagtgattg agcttaagca agcggatgat ttaattatag
     2941 ggcttgcgta ttctgtacaa cgcttagtcg tcgatcattt acacgttgtc ggcgatattt
     3001 atgatcgtgg cccgcaacca gataaaatca tggacacgct gattaattat cattctctgg
     3061 atattcaatg gggtaatcat gatgtgctgt gggttggtgc gtatgcaggg tctaaagtat
     3121 gtttagctaa tttactacgt atttgtgcac gttatgacaa tctagatatc gttgaagatg
     3181 catacggtat taatttaaga cctttgctaa cattagccga aaaatactat gatgcagata
     3241 atcctgcttt taaacctaaa aaaaggcctg acaaacatga acgtttaact caacgcgaag
     3301 aaagtcagat tactaaaatt catcaagcaa ttgcgatgat tcaattcaaa ctggaaatac
     3361 cggttattaa gcgacgtcca aactttgaaa tggacgaacg acttgtactt gaaaaagtta
     3421 attatgatac taatgaaata acagtatacg gaaaaacata tccattaaaa gatacatgct
     3481 tccaaactgt taaccgtgat aacccagcaa agttactacc tgaagaagaa gaagtcatga
     3541 acaaactatt attgtcattc cagcaatctg aaaaattacg tcgtcatatg tctttcttga
     3601 tgcgtaaagg ctcactttac ttaccttgta atggcaattt actcattcat ggttgtattc
     3661 cagttgatga aaatggtgag atggaatcat tcgaaattga tggtcagaca tacagtggcc
     3721 aagagttatt agatgtgttt gaatatcatg tccgtaaatc atttgatgaa aaagaaaata
     3781 ctgatgactt atccactgat ttagtttggt atttatggac tgggaaatac tcttcactat
     3841 ttggtaaacg tgcgatgact acgtttgaac gatactttat tgcagataaa gcttctcata
     3901 aagaagaaaa gaatccgtac tatcatcttc gtgaagatgt gaatatggtt cgtaaaatgc
     3961 tcagtgattt cggattaaat ccagatgaag gacgcattat taatggtcac acaccagtga
     4021 aagaaattaa tggcgaagat cctatcaaag ctgatggaaa gatgcttgtc attgatggcg
     4081 gcttttcaaa agcttatcag tcaacaactg gcattgcagg ctatacattg ttgtataatt
     4141 cattcggaat gcaactcgtt gcacatcaac aatttaacgc aaaagaaaaa attctttccg
     4201 aaggtatcga cgaactgtca attaaacgga ttgttgataa agaattacaa cgtaaaaaaa
     4261 tccgcaatac aaataaaggt aaagagcttc aggctcaaat agatattttg aaaatgttaa
     4321 tgcatgatcg ttacttagac taaagacgac ttttgatgca cgtgtcgtac acttatttag
     4381 aggtgctgtg tttacgcatt ggctacgcaa acgcgatggt aaataggcga cctagctaca
     4441 ccgaaaaaat cccctcacta ttacgagtag tgaggggatt ggtgtatcta tgctagttcc
     4501 ttgttaactg tattattaca acattacagt aaaaaaatgc aaaattaact aataaattaa
     4561 taaaatgatc gacgcccttg cttctactcc aatttaacaa aatactatat tagtactata
     4621 tttttcattc acatgtatgt tacattttct atattactta aaattacgaa gggacatttt
     4681 taaatcatgc gcatcttaaa agagtccatt attgtggcat ttgcctttgt tggtgttgtc
     4741 gttggtgccg gctttgctac tggtcaagaa attttccagt ttttcacaag tcatggcgcg
     4801 tatagcattt caggcattat tgtgacagga ctattgatta ctttaggtgg aatgattgtc
     4861 atgcatacag gtcatcatct aaagtctaga aatcattctg attcaattaa ctatttctta
     4921 tatccctcta ttgcaagagg ttttgatatt attttgacaa tgtttatgtt gtctttagct
     4981 attattatga ctgcaggtgg cgcgtcaacc attcatcaaa gtttcaactt accgtattgg
     5041 ctgagcgcac tcatattagt cgtgtttatt ttagcaacac tgtttctaaa attcgatcgt
     5101 ttaattgctg tgcttggcgg tgttacacca tttttaattg cgattgtcat tatgattgcg
     5161 gtctactatt tcacaacgag tcattttgat tttactgccg ctaataatga tgctcagatt
     5221 cataagcaga aatcattatc acctggatgg tggtttgatg cgattaacta tgcaagcttg
     5281 caaattgctg ctgccttcag cttcttatca gtgatgggta gtaaagttaa gtatcgtgac
     5341 tcaacgttat acgggggctt gattggcggt ttaattatta catttttact catgatgatt
     5401 aacctaggtt taatttctca attcgataaa attaaacacg tcgatttacc tacattaaaa
     5461 ttagcgacac aaatgtctcc gtcaatcggt attattatgt ctgtcattat gatacttgtc
     5521 atctacaata ctgttgttgg attaatgtat gcatttgcgt cacgtttcag cgttccattt
     5581 agcagacgtt acttcatcat tattattaca atggctgtca tcacttatat tagtacattt
     5641 atcggtttca tttcattaat tggaaaagta ttccctatta tgggattgtt cggtttcatc
     5701 ttactcatac ctgtactcta taaaggttta attaagcgta ttaccggcaa atctcatatc
     5761 gattaaaata tctatactat tgtaaaattt agtacaacac ttcaaaatca aaaaagcgga
     5821 cagatgaaaa gtaaaaatct tttccatctg tccgcttctt aatagaactg ttatttctca
     5881 agtatttgtt gaccagccgt taatccagtt tctgtaattt catgaccatt cacccaaact
     5941 tcttcgactt gtgccccacg tgtcttaaac aagttaatga cttgttcact tgcagctaat
     6001 ggcactattg gatcatgttt ccccatagaa agcaacacac tgacatctga taacttcttt
     6061 gttgacctta cttcaattgg atataacggt gcatataaca atgctttttt aaatggtgct
     6121 tctgaacgca acattaagtt aatcgctata tttgatccat ttgaaaatcc aacaagtaca
     6181 gccttttcaa tatcaaaatc ataacgttca gcagcttctt taataaacgt taacaattct
     6241 tgtccccgaa atgccaaatc ttcttcatca taaacacctt caccaagacg tttgaaataa
     6301 cggttcatcc cattttctga aacttgtcct ctaatactta acaagtgata attttcattc
     6361 aatgcttcgc ctaatggtaa taaatcgaac tcatcaccac ctgtaccatg caataatatt
     6421 agcgtcggcg caccattttg tccttctcta aaaatatgtt ccatcacaaa tccctctcat
     6481 tccaattaat catgttgacg cttcgtatta aaaggtctaa cttccgattc tatatattct
     6541 cttttatttt ctaaaaatgg tggtaaggat aacccttctc ctaatgtttc ataaggttca
     6601 tcttccataa accctggtcc atctgttgaa atttctatta aaatatgccc cacacgtgca
     6661 tataatgctt caaaataaaa acgattaacg atgcctgagt tgttaatacc tacctcttta
     6721 tatttcgttg cccacgcttc tattgcatca tgatctttca cacgaaatga cacatgatgt
     6781 acctcaccat aaccttgacg tgctgctggc cctttatcat cttttattaa gattacctgg
     6841 ccaccattgc ctccttcgcc aacttcaagt aatgcgatat tatcttcatg cgcaatagtt
     6901 gtcataccgt aaacagtctc taaaatattt ttaaagtcgt caaaataact tactttaatt
     6961 tcaatggggc ctaatccata aatcgctttg tctactggaa ccggtccatt cttccaaggt
     7021 acaccaggtg ccaccccttc atttaactca tctgaaatta attgatacac ttggccatcg
     7081 acttcttcaa atggcaacac ttttttacca aataattctt gaataccttc atgtttaaca
     7141 ccaaattcat caaagcgctg ttcataatac attaacgcgt catcgttagg cactctaaaa
     7201 gacggtcttg taatggaatt tgttcctgcc tgcccttttg taatatttgg aaaatcaaag
     7261 aacgtcatgt ctgtacctgc cgaaccaacg tcatctgcaa aaaatgtatg atacgtataa
     7321 atatcatctt gattgactgt ctttttaact aaacgcattc ctaatacttc tgtaaaaaat
     7381 ttataattac gttctgcatc atctgtcatt gctgtgacat ggtgaatacc tagtaattca
     7441 tgattatttg tcattttata taccctcttt atatttttag tgtatttatc tcgaaaccga
     7501 gatattttaa tgtaaaattt tagtgcacgc cttacacttc tgtagattgt gcacttagtt
     7561 tcttaaacgc ttgttgtaag attgttaatt catcctttgt taacacatca aacgcttttg
     7621 ttaatgtctc agcatgctta gggaaaatat ctgccatttg gctttgacct ttttcagtta
     7681 aacaagccat atatacacgt ttatcatctt tatccttttc acgtgtaatc caacctttgt
     7741 cctctaattg acttacaaca tatgaaatgc tgctacttgc gattaatacg cggtctctaa
     7801 tacgttgaat tggttgcgga cctttattat aaagcaactc gagcactgca aattcagtaa
     7861 tatttaagcc gtatcgcttt acgtcttctt ttgtaatttg ctctaatgtg tctaacgccc
     7921 tattcattcc gacaaagaca ttgagcgatt gtttcgttcg atccatatca tcaccaactt
     7981 tttaatttat ttcgatttcg agataaatta tatagctgaa tgaatttaat gtcaagatac
     8041 gtgttttcat ctttatttca aattgcactt aataaaaacg actacataga tgtggcaatg
     8101 tcgtcacatg actgtcatgc acctttgact tcgtttaagt taaaatagaa tagggaaata
     8161 ctataatcaa tcatgatagg agttgatatg tatgagtaac cttgaaatta aacaaggcga
     8221 gaacaaattc tatattggtg atgatgaaaa tcatgcttta gctgaaatca cataccgttt
     8281 tgtggataat aatgaaatta acattgatca tacaggcgta tctgatgaac tcggtggtca
     8341 aggtgttggc aaaaaactag ttaaagcagt tgttgaacat gctcgcgaaa atcatttaaa
     8401 aatcattgcc tcatgttcat ttgccaaaca tatgttagaa aaagaagatt catatcaaga
     8461 tgtgtatctt ggttaaataa acctcgcgat ggtgcgccag tgccatcgct tttttatttg
     8521 ttcgtatcaa tataaaattg cgaaacatca tgttattttg catttcaaac tttttgctgt
     8581 caattcattt tcttttctgg tatacttttt atactaactt atagaaaaat agaaaacgag
     8641 gcgattcata atggcatttc acgataaaac agcaacacaa gtgacaaaca ttgaactgaa
     8701 cgtaagagac ttagacttaa tgacaacatt ttacaaaaac attttaggac tatccgttaa
     8761 aagttctgac gataacacaa ccgtattatc tgttgggact ggtggccata ctctaacgtt
     8821 acatttatta gaagacggcc gtcagacttc cccacgagaa gcaggacttt ttcatatagc
     8881 atttttatta ccaactactg aggatctagc taacttctta tatttcgtgg cacaaaaaaa
     8941 tatgggcatc ggcgctggtg atcatttagt aagtgaagct ttatatttca acgaccccga
     9001 aggtaatggt attgaagtct atcgcgatag accttcatct tcatgggaat ggcaaaatgg
     9061 caaagttaaa atggatactt tagaagttga tagccaaacc ttattaacac atcgtactga
     9121 tgaaggttgg caaggaatgc cagcaaaagg catgattgga cacttacatt taaaaacaca
     9181 tgacttagac gcagcatatc aatgttacat tgaacaatta ggattccaac atgtgtctga
     9241 ttttccacgt gcgctattta tgtcaacgaa tcattatcac catcatattg ctgctaatac
     9301 ttggcaatcc aatcaagcaa gacaaaacaa tgcacaaagt tatggattaa cacatgttga
     9361 tatttatcaa cccgacgcct caacacatac atttacggca ccagaagggt ttgacatcac
     9421 agttcattca aatactgacc ttgttccaga aaaataatca tccatacttt attaatacaa
     9481 ttttttcacg caaaagcctg cgattcattt atgtaatcgc aggctttttt tattgctaac
     9541 tttttaattt tatatattta gacatactgt attctattta ttcttttggt ccaacccatt
     9601 caataacatc ttcatatgct tggcgtgttt tatttttcgg tggatcttgt tgtctgtagc
     9661 caaatgcaac catcactgat aaaccaaatt gctcagtatc taagatacct ttatttgcta
     9721 aaatgtctgt cactgtatcc agactaaaac cttccatcgg acatgaatca atacctaaca
     9781 atgcggctgt cgtcatcata ttgcctaatg cgatatacgt ttgtttactt gaccagtcat
     9841 acaaggcttg atcattatca gaaatatgga aatctgcttg gaatgcatcg aatttttgtt
     9901 caacagctgg aatcgtttgt gcctcatatt ttttaatatc tcttaacata tgttgtacat
     9961 acggtgatct tgacgttaca tttttacgcg caaaaattag cacaaaatgg ctcgctgtat
    10021 ctaattgttt cgctgcgccc caactgtgcg cttttaattc atcacgtaac gcttgatctt
    10081 gaatcacgac aaacttccaa ggttctaacc caagagaact tggagacaat ctacctgact
    10141 ctaatattgt ttcaaaatct tctttcgaaa ctttcttttg tggatcgaat tgcttagtag
    10201 catgtctgaa atgaaatgca tccataattg tttgattcat attgctcatt gaacatagcc
    10261 tccaattttt atatttattg ctatacaaat tatacttcat gttttaaaaa gactaaagta
    10321 atccgtttgc aattcactat aacttgtgca catgttataa gttttctgca ataaaacaca
    10381 cttttgaatt ttggaaaatt tagtgttata atatatttgt gatatttttc acaattaaat
    10441 tgtggcgtga ttttaatgtg cataatcttt aatttcactc atttactttt caatctttta
    10501 aaagcgaggt ttttaataat gacaaaaatt atgttctttg gtacgcgtga ttatgagaaa
    10561 gagatggcat taaattgggg gaaaaagaat aatgtcgaag taactacttc taaagagcta
    10621 ttatcaagtg ctacagtcga tcaattaaaa gattacgatg gcgtaactac aatgcaattt
    10681 ggtaagttag aaaatgacgt ttatcctaaa ttagaatctt acggtattaa acaaattgca
    10741 caacgtactg ctggatttga tatgtatgat ttagatttag ctaaaaaaca caacattgtg
    10801 atatctaacg ttcctagtta ttcacctgaa acaattgcag agtattctgt ttctatcgcc
    10861 ctacaattag tgcgtcgctt cccagatatt gaacgccgtg tacaagcaca tgattttact
    10921 tggcaagcag aaatcatgtc taaaccagtt aaaaatatga ctgttgcaat tatcggtacg
    10981 ggtcgtatcg gtgctgctac agctaaaata tatgcaggat ttggtgctac aattacagct
    11041 tatgacgctt atcctaataa agatttagac tttttaactt ataaagatag tgttaaagaa
    11101 gctattaaag atgccgatat tatttcttta catgttccag cgaacaaaga aagctatcat
    11161 ttattcgata aaacaatgtt tgatcatgtg aaaaaaggtg caatcttagt taacgcagca
    11221 cgtggtgcag tcatcaatac acctgattta atcgatgcag tgaacgatgg tactttgtta
    11281 ggtgctgcga ttgatactta tgaaaatgaa gcagcatact tcacaaatga ctggactaac
    11341 aaagacattg acgataaaac attattagag ttaatcgaac atgaaagaat tttagtaaca
    11401 ccacatattg ctttcttctc tgatgaagca gtacaaaacc ttgttgaagg tggtttaaac
    11461 gcagcattat ctgtcattaa cactggtaca tgtgaaacac gtttaaatta atatatattt
    11521 aaacaggctg ggacataaat ccctaaaaaa cagcagtaag ataattttca attagaagat
    11581 atcttactgc tgttctatat ttatacaata cttcgtattg aatggcttcg ctatgcccat
    11641 ctggcacatt actgtaaaat tctataaata gaatttttga tgatgggtcc cttcctaggg
    11701 tgccgtctca gcctcggtct tcgactggca ctgctccctc aggagtctcg ccattaatac
    11761 tacgt
//
LOCUS       DATTQF010000079        11514 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.079, whole genome shotgun sequence.
ACCESSION   DATTQF010000079 DATTQF010000000
VERSION     DATTQF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.079"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..973
                     /locus_tag="VB241_001734"
     CDS             <1..973
                     /locus_tag="VB241_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245026.1"
                     /translation="LVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELN
                     QENVKNHYMWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAI
                     WNFLFLRRALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSV
                     DLDKIRAKRDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEF
                     NNRNALFVSGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGS
                     ILFFKLLTEFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            1398..2570
                     /locus_tag="VB241_001735"
     CDS             1398..2570
                     /locus_tag="VB241_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP1245027.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEVNTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRIFSVQKDYDKLITGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            2624..3166
                     /locus_tag="VB241_001736"
     CDS             2624..3166
                     /locus_tag="VB241_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEP1245028.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRER
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            3320..3586
                     /locus_tag="VB241_001737"
     CDS             3320..3586
                     /locus_tag="VB241_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP1245029.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            3589..5307
                     /gene="ptsP"
                     /locus_tag="VB241_001738"
     CDS             3589..5307
                     /gene="ptsP"
                     /locus_tag="VB241_001738"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP1245030.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNNAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDLELIQPIQDKIKNENANA
                     ASALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEAKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(5544..5777)
                     /gene="nrdH"
                     /locus_tag="VB241_001739"
     CDS             complement(5544..5777)
                     /gene="nrdH"
                     /locus_tag="VB241_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEP1245031.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNERQIDFEERNIKNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            5975..7336
                     /locus_tag="VB241_001740"
     CDS             5975..7336
                     /locus_tag="VB241_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEP1245032.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRNFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYTILMYTCAYVLIRMFKNKPAYEDVNRLVKKQGGE
                     IEK"
     gene            7333..8352
                     /locus_tag="VB241_001741"
     CDS             7333..8352
                     /locus_tag="VB241_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEP1245033.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            8485..9147
                     /locus_tag="VB241_001742"
     CDS             8485..9147
                     /locus_tag="VB241_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP1245034.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(9495..11192)
                     /locus_tag="VB241_001743"
     CDS             complement(9495..11192)
                     /locus_tag="VB241_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP1245035.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(11192..11410)
                     /locus_tag="VB241_001744"
     CDS             complement(11192..11410)
                     /locus_tag="VB241_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP1245036.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
BASE COUNT         3873 a         1627 c         2075 g         3939 t
ORIGIN      
        1 cttagttatc ggtatcatgt actttattat tacgcttata ttaatgacgc aagtatatgg
       61 attctttgcg gtaattttca ttattccatt tacaattcat attggatata tcgtttataa
      121 agatgaattg aatcaggaaa atgttaaaaa tcattacatg tggattattg taagttatgg
      181 tataagttat ttaattacac aaattgcatt gtatggcaga attgatgcta atgaaataga
      241 atcaattgat atcttaagtg tcaatgcttt ctttataatt atgtggttac ttggtcaaat
      301 ggctatttgg aatttcttgt tcttgcgccg agctttacct ttaacaaagc aagaattagg
      361 tgaagaggag ccagaattat caagaacaag taaagggaat gtcacgaatc aaactaaaat
      421 tcacttgaag caactccaag ataagactac agaatatgca cgtaagacaa gaagaagtgt
      481 cgatttagat aaaattagag ctaaaagaga taaattcaaa aagaaagtta atgatattat
      541 cgatattcaa gaggacgata ttcctgattg gatgagaaaa ccgaaatggg ttaaaccgat
      601 gtatgtcgaa ctattttgtg gtgtcgtcat ctttttattc acatttttag aatttaataa
      661 tcgtaatgca ttatttgtat ctggtgattg gaaattatca cagacacaat atgttattga
      721 atgggttaca ttactaattc tgctattcat tattatcgca tatatcgcta caacgttaac
      781 tttccacttg aaaggtaagt tttattattt acaattattt atggggagca ttttattctt
      841 taaattgtta acggaattta taaatataat gattcatgga cttctacttt cagtgttcat
      901 tacgccaaca ttactattaa tgttattggc aatcatcatt tcttattcgt tacaattacg
      961 agagcgacca taattaaaag cattataaaa gtactatcta ttaaacattt tgatgtgtac
     1021 gctataagtt agatatatct ctaacttact tagatacagg tcaatgaagt ttatggatag
     1081 tacttttttt gtaactagat ttgattgatt caggtaatgt gaattaagta ttgaaaattg
     1141 tatacaaagt tttagtgcaa ataaaatagt tgaaaagtta tccatttgta aaattaagaa
     1201 aactagtaaa tagttgaagt gacaagtgga atttgcgaaa cgatatatag tatctcattt
     1261 atagaagttt aacatatatc attcgaatta ctaatttgtt aaaatcaaca gtaagattag
     1321 aagtagatga tattgaaatt tggcaaacaa tttaatctat ataaaactac aacgaaacac
     1381 agaaaggaag ttgtcagatg aaaatagcaa ctctgaacaa aggcaaagaa acaaaatatt
     1441 ttaatggata tcctttaatt gaagaagagg atatctattc acaagatcat ttaaaagaag
     1501 gagatatttt tcaaattgtg actgataaat cacaatatgt tgcaacggct tatgttggtc
     1561 gccaacataa aggattaggt tgggttctaa catacgataa agctcaagaa gtcaacacag
     1621 ctttctttgt gaaattgttt aatactgcat tagcagaacg tgattattat tttaatatag
     1681 atggaacaaa tgcttttaga ttatttaatg ctgaaggtga tggagttggg ggattaacga
     1741 tcgacaatta cgatggtcat ttgttgattc aatggtactc aaaaggtatt tataaattta
     1801 aatatgccat tcttgaagcg gttagaaagg tatttgatta taaatctata tacgaaaaag
     1861 taagatttaa agacagcgaa tatagtggtg gttttgttga aggcgatgca cctgagtttc
     1921 caattgttat cgaagaaaac ttcacatttt ataatgtaga ccttgaagat ggtttgatga
     1981 caggtatctt tttagatcaa aaagaagtgc gcaagaaatt aagggatcaa tatgccaaag
     2041 aacgccatgt tttaaactta tttagttata caggtgcttt ttctgtaata gcagcaagtg
     2101 aggcatcttc aacaacaagt gtagatttgg ctaatcgttc tcgtagttta actgaagaaa
     2161 attttggatt aaatgctatt gatcctaaat ctcaatatat ttatgtcatg gacacttttg
     2221 atttctataa atatgctgca cgtcatggac atagttatga cacgatcgtg attgatccac
     2281 ctagctttgc acgcaataaa aaacgtatat tttcagtgca aaaagattat gacaaattaa
     2341 ttactggcgc cttaaatatc ttatcatctg aaggaacatt attattatgt acaaacgcaa
     2401 gtgtatatcc attaaagcaa tttaaaaata ctattaaaaa gactcttgaa gagagtggcg
     2461 ttgattatga attaactgaa gttatgggat taccaaaaga ttttaaaaca catccacatt
     2521 ataagccatc taaatattta aaagctgttt ttgtaaatat tagacattaa tttgataaaa
     2581 aggggtatag tatcttattg agaaaaagaa gggtgatgat acaatgggat tcaaaaataa
     2641 tttaacatca aatttaacaa ataaaattgg taattcagtt tttaaaatag aaaatgttga
     2701 cggaaaaggt gcaatgccaa cgacgattca agaattgaga gaaagacgcg aacgtgctga
     2761 agcaattgta aagagaaagt ctttaatgtc atcaacaatg agcgttgttc caattcctgg
     2821 attagacttt ggtgttgatt taaaattaat gaaagatatt atcgaagatg taaataaaat
     2881 atacggatta gatcataagc aagttaatag ccttggggat gatgtgaaag aaagaattat
     2941 gtctgcagcg gcaattcaag gtagtcaatt tattggtaaa agaatttcaa gtgcattttt
     3001 aaaaattgta attagagatg tagcaaaacg aactgcagct aaacaaacaa aatggtttcc
     3061 tgttgtagga caagctgtgt ctgcatctat tagttactat tttatgaata aaattggaaa
     3121 agatcacatt caaaaatgcg aaaatgttat taaaaatgtc atgtaggtgc tataatagtt
     3181 ttgcaatttg caaattttac tgaaaccggt tttaaacgaa ttgaatttaa agcatggttt
     3241 tggtaaagtt aaggtataaa actaagttag tattgtaata atattgaaga ttctaactat
     3301 acgaaggaga aatgtaatta tggaacaaaa ttcatatgta atcatcgacg agactggtat
     3361 tcacgctaga ccagcaacaa tgttagttca aacagcttca aaattcgatt ctgatatcca
     3421 attagaatat aacggtaaaa aggttaactt aaaatcaatc atgggtgtta tgagccttgg
     3481 tgttggtaaa gatgctgaaa ttacaattta tgctgacggt agtgatgaat ctgacgccat
     3541 tcaagcaatc agtgacgtct tatcaaaaga aggattgact aaataatcat gtctaaatta
     3601 attaaaggaa ttgctgcatc tgatggtgtt gcaattgcta aagcttattt gttagttgag
     3661 ccagacttaa cattcgacaa aaatgaaaaa gtcactgatg ttgaaggaga agttgctaag
     3721 ttcaataacg ctatcgaagc ttctaaagtt gagttaacta aaattagaaa taatgcagag
     3781 gttcaattag gtgctgataa agctgctatc tttgatgcac atttattagt tttagatgac
     3841 cttgaattaa ttcaaccaat tcaagataag attaaaaatg aaaacgctaa tgctgcttca
     3901 gcattaacag atgtaacaac acaatttgtt acgatttttg aatctatgga taatgaatac
     3961 atgaaagaac gtgcggctga tattcgcgac gtttctaaac gtgtgttatc acatatttta
     4021 ggtgtagaat taccgaatcc gagtatgatt gatgaaagcg ttgttattgt agggaatgac
     4081 ttaacgccat cagatactgc tcaattaaat aaagaattcg tacaaggttt tgctacaaac
     4141 attggcggaa gaacaagtca ctctgcgatt atgagtcgtt ctttagaaat tccagcaatt
     4201 gttggtacaa aatcaattac tcaagaagct aaacaaggcg acatgattat cgtagatgga
     4261 ttaaatggtg atgtaatcgt taatccaact gaagatgagt taatcgctta tcaagataaa
     4321 cgtgagcgtt attttgctga caagaaagaa ttacaaaaac tacgtgatgc tgatactgtt
     4381 acagttgatg gtgttcacgc agagcttgct gcaaatattg gtacacctaa tgatttgcca
     4441 ggtgttattg aaaatggtgc acaaggtatc ggcttatata gaactgagtt tttatatatg
     4501 ggtcgtgacc aaatgcctac agaagaagaa caatttgaag cttataaaga agtattagaa
     4561 gcaatggacg gtaaacgtgt tgttgtacgt actttagata taggtggaga taaagaatta
     4621 tcatacttaa acttgcctga agaaatgaat ccattcttag gttaccgtgc gattcgttta
     4681 tgccttgcgc aacaagatat tttcagacca cagctacgtg cattattacg tgcatcagtt
     4741 tatggtaagt taaatatcat gttcccaatg gttgcaacaa ttaacgaatt tagagaagct
     4801 aaagctatat tattagaaga aaaagaaaac cttaaaaatg aaggtcatga catttcagat
     4861 gatatagaat taggaatcat ggtagagata cctgcaacag cagcattagc tgatgtcttt
     4921 gctaaagaag tagatttctt cagtatcggt acaaatgatt taattcaata cacattagct
     4981 gctgaccgta tgtcagagcg tgtatcatat ctataccaac catataatcc ttcaatctta
     5041 cgtttagtta aacaagttat tgaagcgtca cataaagaag gtaaatggac aggtatgtgt
     5101 ggtgaaatgg ctggagatga aacagctatt ccattattgc ttggtttagg tttagatgag
     5161 ttctctatga gtgcaacgtc tattctgaaa gcaagaagac aaattaatgg tttaagtaaa
     5221 aatgaaatga ctgaacttgc taaccgtgca gtcgactgcg caacgcaaga agaagttatt
     5281 gaattagtta acaactacgt aaaataatta attctttcga aagacagaat tataaataaa
     5341 cccgaagtat tcattgtgag taattatgaa tacttcgggt tctttttttg atttatgtta
     5401 atttcaatgg aatgttgttg ctaactaatt aaacaactag gtgtcattga cataaatatt
     5461 gttctttaat taatgttagt taatttaaaa atcgatattt aatttatgat agctaaaaat
     5521 gatgaagtta atttagatga aaattaatct tggatattta atactttgtt gatttcatca
     5581 agatcaacat ggtacattgg attgccattc aacaaaataa acggagttga aaaagcatca
     5641 aaatctatca tttcgtttcg atattgttga tttttgatat ttctctcttc aaaatcaatt
     5701 tgacgctcat ttagataatt ttttacaaat gtacaaggtg gacaatcatt ctgtgtataa
     5761 acgattattt ctgacatgta tcatgctcct tcgtgcttta ttctaatgta atatacacaa
     5821 ttatacattg aaattcaaat ttgtgaacac attgtgaact gacataaatt atacacaatt
     5881 tcatagcgca taatgtttta aaaatacaca ttatagcaat tataattaag ttcattgtgt
     5941 tgtcatcaaa aaaagaaaaa ggtgatgttt ttaaatggat acagttgaaa tcagtcggtt
     6001 tttgacagct atgactttag cagttcatat catttttgca acgattggtg ttggtatgcc
     6061 tttaatgttc gcaattgcag aatttttagg tattcgcaaa aatgatcttc aatatatagc
     6121 tatggccaaa agatgggcta aagcttatac aattactgta gcagtgggag ttgttacagg
     6181 tacaattata ggacttcaat tatcattgat ttggcctaca tttatggaaa tgggtggaca
     6241 cgttattgca cttccattat ttatggaaac atttgcgttc ttctttgaag ctattttctt
     6301 aagtatatat ttatatactt gggaccgttt taaaaataaa tggacacatt tcttaattag
     6361 tataccagta attattggtg gctctttctc agcattcttc attacttcag tgaattcatt
     6421 tatgaatacg cctgcaggtt ttgagttgaa gaatggaaag atggtcaatg ttcaacctat
     6481 agaagcgatg tttaacccat cgtttatagt tcgatcattt cacgtaatta ctacagccgg
     6541 tatgacgatg gcatttgtta ttgcatcaat agcagctttt aaattattgc gtaatcgtca
     6601 acctaaggat actgtctacc ataagaaagc tttgaaaatg tctatgattg ttggattctt
     6661 ttcaacttta ctttctatgt tggcagggga tttatctgca aaatttttgc ataaattcca
     6721 acctgaaaaa ttagcagctt atgaatggca tttcgataca tcttcccatg ccaaattatt
     6781 attatttggt gtgttagatg aaaagactca gcaagttaaa ggtgcgattg aattacctgg
     6841 actattgagt ttcttagcag ataatagtgt caaaactaaa gtgcaagggt taaatgattt
     6901 tccgaaaagt ttacatccgc ctatgatcgt ccattatttc tttgatttaa tggtaacgat
     6961 gggaatttta tgttttgtca tttcaggtgt ttatgtttta actttaatgt ttaaaaagct
     7021 tagaaatttt tctactcata aatggatgct ttacggaata ttattaacag gaccagcttc
     7081 aatgctagct atagaatttg gatggttctt aacagagatg ggtagacagc cttggattgt
     7141 tcgtggttat atgcgcgtgg cagaagcagc aacacaagca ggcggaataa cctttgttac
     7201 aattttattt ggcatattgt acaccatttt aatgtataca tgtgcatacg tattaattcg
     7261 tatgtttaaa aataaaccgg cgtatgaaga tgtaaatcgt ttagtcaaga agcaaggagg
     7321 agaaatagaa aaatgattta tgcatttata ggtataacag tgttatggtt gtttttattt
     7381 tgctatatca ttattgcttc tatagatttt ggggcaggtt tcttcgcatt gcattcaaag
     7441 ttaactggtg atgaaaagaa aattaatcac ttaatttcac gttatttgaa cccagtttgg
     7501 gaagttacga atgtattctt tgttttcttc ttcgtaggat tcgtaggttt ctttccagag
     7561 tcaatcaaat atttaggcac ggtattgtta ataccaggtt caatagcact gattatgata
     7621 tcgttgagaa acagttttta tgcatttgaa aattatggtc aagatacaaa attagcatgg
     7681 atgatcatgt atggggtaag tggattgtta attccagctt cattatctac tgctttaact
     7741 attacagaag gtggttatat taatgttcga aacaatgtta tcgatctaga ttgggtgcag
     7801 ttactgttaa gcccatttgc ttggtctgta gtattcttgg caattatttc agttttatat
     7861 atttcatcag gatttttgac atattatgct aaaaaagcaa atgacgaacc agcatataat
     7921 ttaacaagac aatggcacat atttttaggg ccaccgatga ttatcatttg tttattcgta
     7981 tttctatcat tacgcattca aaattctgaa catttttatt cagctgtttt tgactactgg
     8041 tggatgtttg ctataagttt cctattcttt gcattagctt cattgttaac attctttaag
     8101 aaaaaacatg gtttggcttt tgtatttgtt attttacaaa tgatgttcgc gttctttggc
     8161 tatggaatta gtaaattgcc atacctttta tatccgtttg taaaaattac agatgcatat
     8221 gttaatccag aaatgggctg gacattagtg attgtcttta ttttaggttt acttttatta
     8281 cttccatcgt taatattatt attaagatta tttgttttcg acaaagaata tgttgaagga
     8341 aagaaatcat aagttgagac gataccccaa ctttcattta cttgcattac tgcatttaga
     8401 taatttagtt ggggtactca tttataataa ttttaaattt atattttcaa tgaattgatg
     8461 aaaatcattt aatggagggc gagtatgggt aaagaatatg tagtcatcgg tctaggtcgt
     8521 tttggaggta gtatcgttcg tgagttgaat gcattagaca tggatgtaat ggccatcgac
     8581 catgatgaaa atagagtgaa tgaatatagt gatatcgcta ctcatgcggt tgttgcagat
     8641 actacagatg aagcagttat gaaaagttta ggtatccgta actttgatca tgtcattgtg
     8701 gcaattggtg aaaatattca atcaagtacg ttgacgactt tgattttaaa agagttaggt
     8761 gtaaaaaaag taactgctaa agcacaaaat gattatcatg caaagatttt aaataaaatt
     8821 ggagcagata cggttgtgca ccctgagcgt gatatgggta gacgtattgc gcataatgtt
     8881 gcgagtgcaa gtgtacttga ttatcttgag ttggcagacg agcattctat tgtagaattg
     8941 aaagcgactg aaaagatggc ggggcagtct atcattgatt tagatataag agcacaatat
     9001 ggaattaaca ttattgcaat taaacgaggc aaagagttca tcatttcacc aaatccaaat
     9061 attaatttag aaataggtga tattttaatt atgattggac atgataatga tttaaatcgc
     9121 tttgaaaaaa atattgcgac gagataatcg ggagtggtac agatatgata ttttctcaaa
     9181 atttatttcg tcgtcccacc ccaacttgca ttgcctgtag aatttctttt cgaaattctc
     9241 tgtgttgggg cccatcccca acttgcacat tattgtaagc tgacttttcg tcagcttctt
     9301 tgttggggcc cctgactaga attgaaaaaa gcttgttaca agcgcatttt cgttcagtca
     9361 actactgcca atataacttt gtagagcatt gaacattgat ttatgtctca agctcatcat
     9421 atataaagaa aaagctacta acaatgtgtg gtgagtttaa ttaaaacttc acttcaaagt
     9481 tagtagcttt ttgattattt attgtttgat tctttttgtt cgtttacctt cataatgact
     9541 ggtaaaatca ttggttttct agctgttttt tcaaataaat aaggttgtaa tgtttcaatg
     9601 atagaagatt taatctgatg ccattgaata tctttatttt gatttaactt actaataaca
     9661 tcagttttga ttttgcgttg tgcatcataa attaattgtc ctgattctct catatataca
     9721 aatcctcgag aaataatatc tggaccagaa agtaatttat ttgtattaaa atcaatgcta
     9781 acaacaacga taactaaacc ttcttcagat aatagcttac ggtctcttat tacaacatta
     9841 ccgatatcac cgataccact accatcaaca agtacattac cagatggaat gcgaccagct
     9901 ttacgtgctg aatcgtgtgt taaagctaag acatctccga tatcaaagat gaagacatta
     9961 tcttcttcaa cgccgcattc aacaccagtc tcaccatgtg cttttaacat acggtattca
    10021 ccatgaatag gtaagaaata tttcggcttg attaatcgaa gcattaattg ttgatcacct
    10081 tgagaaccat gccctgaagt atggatgtta gaaatcttgc tgtggataac atctgcacca
    10141 gctttataca aggaattaat cgttctgtta atactttttg tattacctgg gataggtgat
    10201 gaactaaata caacggtatc ttcaggtata attttaattt gcttatgagt accattagca
    10261 attctagata atgctgccat tggttcacct tgagaaccag tacatagtat taataactca
    10321 tgcttcggta cagtattaat tttattaggt tcaataaatg tttcaggtgg tgctttaata
    10381 taaccaagtt ccataccgat tttaatattg ttttccatcg aacgaccgaa cgtaacaatt
    10441 ttacggttat ttttgatagc agcttcaact gcttgttgaa ctcggtaaat attagaagca
    10501 aaggtagcaa atataatacg acctttacaa ttgcggaaga tcttatctac gttttgacca
    10561 acttcacgtt cgcttaaagt aaaatcaggc acaagtgaat ttgttgagtc tgaaagtaaa
    10621 catagaacgc cttcttcgcc taattgagcc attttagcaa tgtttgctgg tttgcctaca
    10681 ggtgtaaaat caaatttaaa gtcaccggta tgaactactt ttccttcagg tgtatctacg
    10741 atgacgccat aagtttcagg aatactatgc gtagttaagt agaaagaaat cgtaaagtgc
    10801 ttagatttaa tcacactgtc ctcattgatt tcatttagtt tagcagtacg taataaatga
    10861 tgttcttcaa gtttattacg gattaaacct aatgctaaag gaccaccata aataggtata
    10921 ttaagttgtt ttaataggaa gggcacaccg cctatatggt cttcgtgacc atgtgttata
    10981 aataggccaa caattttatc ttggttttga actagatatg tgtagtcagg tataacataa
    11041 tcaatcccta ataagttatc atcagggaat ttgatacctg catcgataat gacaatttcg
    11101 tctttatact caactgcata agtattttta ccgatttcac ctagacctcc aagtgcatat
    11161 acacctactt catttggatg taattgtttc attatttagc aatctccaca ttaaagtgtt
    11221 ctgagttttc tttttcgtaa tctaaatgtg cgccctctaa tttagtgata aattcgatat
    11281 taaaattacg atctttcaag taacgacgta cttgttcttc tgtttgagct tcaacataaa
    11341 gtgattgtgt attttcacgc acaattacct cgtctctgtt atgttgataa aaaactttaa
    11401 atactgccat gttaaaaatt cctcctaaga atgtttgttt aatttattgt taaccctagt
    11461 aaaatcgtat tggagtatat atcgataaat tcattccaat catctctata atgt
//
LOCUS       DATTQF010000080        11451 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.080, whole genome shotgun sequence.
ACCESSION   DATTQF010000080 DATTQF010000000
VERSION     DATTQF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.080"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..523)
                     /locus_tag="VB241_001745"
     CDS             complement(<1..523)
                     /locus_tag="VB241_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245037.1"
                     /translation="MIRNRLSELLSERGLKISRVAKDVKIARSSLTSMAQNDSEMIRY
                     DAIDKLCSYLHISPSEFFEHNPINFDFTFDEEPNYKINDVFEGFEVTANITHAFSIEN
                     FDFEILVDVELDNRQKLNFDLDVSYKETEKITNSQHRFIFTIKNEDENIGLKKYVDSL
                     SAGLKNLLFKKINQ"
     gene            696..914
                     /locus_tag="VB241_001746"
     CDS             696..914
                     /locus_tag="VB241_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245038.1"
                     /translation="MTILANTRKFKEAMFLKGFNLSDLSRETGVGISYLSQIINGKKI
                     PSPKLAKKMAEVLQVEVNELFEFEVKEA"
     gene            919..1236
                     /locus_tag="VB241_001747"
     CDS             919..1236
                     /locus_tag="VB241_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1245039.1"
                     /translation="MFNINIDEDEARELLEQAINARVDELAKEKYFMTYKELSNYLNL
                     SKPTIEELLINNGMKYYMVGSTYRFKKSDVDEFMEQLTAHMNIQNNDFKQVNIKKLLE
                     ARQ"
     gene            1233..1388
                     /locus_tag="VB241_001748"
     CDS             1233..1388
                     /locus_tag="VB241_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245040.1"
                     /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYAWLITLGCNLTGE
                     ITTCENK"
     gene            1373..1576
                     /locus_tag="VB241_001749"
     CDS             1373..1576
                     /locus_tag="VB241_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245041.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVVCLEMDLSKITEIKNKKKVGS"
     gene            1578..1961
                     /locus_tag="VB241_001750"
     CDS             1578..1961
                     /locus_tag="VB241_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245042.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIAGNIEVEEL"
     gene            1962..2279
                     /locus_tag="VB241_001751"
     CDS             1962..2279
                     /locus_tag="VB241_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HEP1245043.1"
                     /translation="MNWEIKDLMCDIEAVKEKINDVAIKHAWFVEDRFVKNELETKRE
                     HINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKFHEIEKASSENFGEVSDDAQKLK
                     ITE"
     gene            2343..3212
                     /locus_tag="VB241_001752"
     CDS             2343..3212
                     /locus_tag="VB241_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEP1245044.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFNSFSKINWSKLVNKLSVPI
                     EANYKYARGVAVYGDIKNGANDQGEIIKKHRNDKNVIYRDVIVLDYDEINDLKQLHEA
                     ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYTKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIMNVDMLEQWSKEFEQSNASPNVIGYTRRDS
                     EYWRELCFGTTEGNRNNALASLIGHLLRCHVNDYIVYSFALLWGQFACKPPMKEQEIN
                     ATFQSILNKHYNN"
     gene            3226..4935
                     /locus_tag="VB241_001753"
     CDS             3226..4935
                     /locus_tag="VB241_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEP1245045.1"
                     /translation="METGKSDVLDKIEKINKKDSALQEIIPKGYEIEHHQCGIALNQL
                     IPSKKEGEPDKKVFITSTIPQITERFEDIESNEVSFNMLFYDNKTPVNIAVSAEEISD
                     SRQLLKLVNKKLDVTSSTSTKLVDYINASKRYNPPLNVKVATRLGHVKGYFIYPYQEV
                     MKDSNIKLFSNDKGFQKLIDSFQSKGTLEGYSKKVFGQIKDLPMVMVMLYASLGSVLL
                     REFGLQPFIVEISGSTSTGKTFTLNLVSSVWGTSDLITTWSSTQNSIESMASFLNSFP
                     MFKDDTRNTHPKFVTSATYNFSSGESKSRSNINLTLNAKKEWRNILISTGESSIANMA
                     DEKAGVSARVVTLQDPPYPDNFDFTTLDKSFRENYGTLGLAFIKQYESKKDVYKNAFE
                     SYQRYFNQKGSNEIMQRLGRAFALLQVTGEVLNDIDGFEHDHFKIIEQAYDSMVKNNK
                     TIDKPKQLLEELLQYLDANRNNIAGDGYSSVKNGDIKAIYKRDYLCILGQTVHDKLGH
                     EMQTITGQWGKKGYLIKGEKDRLQKKVSHKNIKYRGFAINKEMLEELGFDFSNSHNPY
                     SDY"
     gene            5247..5627
                     /locus_tag="VB241_001754"
     CDS             5247..5627
                     /locus_tag="VB241_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245046.1"
                     /translation="MDKEQLKKYIYDYVKEYKEIPIYQLEDLFKEMNHDYIGRTSVTH
                     DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFVMRYLLDGRVPNLPLAICYPE
                     DGQQTDVPSWVPMVLRINKEEKIK"
     gene            5624..6265
                     /locus_tag="VB241_001755"
     CDS             5624..6265
                     /locus_tag="VB241_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245047.1"
                     /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM
                     AYVMMNGKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKQLSISD
                     MMASQLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY
                     QLATGINEPAPELVEGLRLVTEFVQDENATQEDYKALEHKLTELKSSYYSLNK"
     gene            6801..7142
                     /locus_tag="VB241_001756"
     CDS             6801..7142
                     /locus_tag="VB241_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245048.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEY
                     NSSNERWLRRVINSLVYDYGYPIGCSYKPSERGYYIIMTEQEKQQAMRSIKKLADGSM
                     KRYEALKRIKV"
     gene            7154..7732
                     /locus_tag="VB241_001757"
     CDS             7154..7732
                     /locus_tag="VB241_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245049.1"
                     /translation="MKTESYFKEYNQFVIDQQKAIQELKQERNALESKIKIDKSTYKQ
                     LIMDGQDDKADNLYQATDADEKKLKALNKRLETKKSVSKEVKYQKTIELLKHQSELSS
                     LYESEKQSALGKLKKVVDAYNEIIDEIEDINDRYEDEHQQYASIYSQEQLYDDKEARE
                     ALNGYFRENIFTSYINGNDLPYEHNNKLFLKR"
     gene            7750..7968
                     /locus_tag="VB241_001758"
     CDS             7750..7968
                     /locus_tag="VB241_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid morphogenesis B protein"
                     /protein_id="HEP1245050.1"
                     /translation="METKYELNNTKKVANAFGLNEEDTNLLINAVDLDIKNNMQEISS
                     ELQQSEQSKQKQYGTTLQNLAKQNRIIK"
     gene            8019..8546
                     /locus_tag="VB241_001759"
     CDS             8019..8546
                     /locus_tag="VB241_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HEP1245051.1"
                     /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNMALLKKVL
                     NHYTEKEQKQVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSYVKVV"
     gene            8549..8890
                     /locus_tag="VB241_001760"
     CDS             8549..8890
                     /locus_tag="VB241_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HEP1245052.1"
                     /translation="MDKKQIKDFVCDYHKRTRSDVLIDDEINTDEFFSIGDENSNEWM
                     ADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSFKDISNVLECSEQSVRLWYE
                     TLLDKIVEVIE"
     gene            8887..9456
                     /locus_tag="VB241_001761"
     CDS             8887..9456
                     /locus_tag="VB241_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP1245053.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGD
                     DEDLDKTVQEVSNANPNHTVIVDDIPLED"
     gene            complement(9611..10315)
                     /gene="tst"
                     /locus_tag="VB241_001762"
     CDS             complement(9611..10315)
                     /gene="tst"
                     /locus_tag="VB241_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic shock syndrome toxin TSST-1"
                     /protein_id="HEP1245054.1"
                     /translation="MNKKLLMNFFIVSPLLLATIATDFTPVPLLSNQIIKTAKASTND
                     NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV
                     DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFD
                     KKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEK
                     PPINIDEIKTIEAEIN"
     gene            10734..10961
                     /locus_tag="VB241_001763"
     CDS             10734..10961
                     /locus_tag="VB241_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEP1245055.1"
                     /translation="MKQNDLAHGEFGNWLKNINLDRTQAHRFIKVSEEIKDVGTYQHL
                     GLRALSEIASLPVPVRIKVHITSNGKTKIPY"
BASE COUNT         4308 a         1482 c         2120 g         3541 t
ORIGIN      
        1 tttggttaat ttttttaaat aacaagtttt taaggcctgc agataaacta tcaacgtatt
       61 tttttaatcc gatattttca tcttcatttt taatcgtgaa aataaatcta tgttgtgaat
      121 tagttatctt ttcagtttct ttatatgaga cgtctaagtc aaaatttaat ttttgcctat
      181 tatctaattc gacgtctact aaaatttcaa agtcaaaatt ttcaatcgaa aaagcgtgag
      241 taatgtttgc agttacttca aatccctcga aaacatcatt aattttataa ttcggttctt
      301 catcaaaagt aaagtcaaaa ttgatcggat tatgttcaaa aaattctgaa ggagatatgt
      361 gcagataact acataattta tctatagcat catatcttat catttcagaa tcgttttgtg
      421 ccattgaagt aagtgaactt cttgctattt ttacatcttt tgcaacacga gatattttta
      481 gtcctctttc tgacagtagt tcagacaatc tatttctaat cattacaaac ctcctaatta
      541 tgttaataat agcatttttt tggacgttta tgtacaaaaa aataaaaaat gattgagaag
      601 tcagtcgaaa aactattgca aaagaaaaac gattatgtat aataaagtta taaattgatt
      661 gagaagtcag tcaaaaacga aggaggattt taattatgac tattttagcg aatactagaa
      721 agtttaaaga agccatgttc ttaaaaggct ttaatttatc tgatttatca cgtgaaacag
      781 gtgttggaat ttcttattta agccaaatta ttaatggtaa aaagattcca agccctaaat
      841 tagctaagaa aatggcagaa gttttacaag ttgaggtaaa tgaattattt gaatttgaag
      901 taaaggaggc ataaaccaat gttcaacatt aatattgatg aagatgaagc acgtgagtta
      961 cttgagcagg ctatcaatgc acgtgtggac gaattagcga aagagaaata ttttatgact
     1021 tacaaagagt tgtctaacta tctgaattta agtaagccta ctattgaaga attacttatt
     1081 aataatggca tgaaatatta tatggtcgga tctacgtaca gattcaaaaa gtctgatgta
     1141 gatgaattca tggaacagct tactgctcat atgaatatcc agaataacga ctttaaacaa
     1201 gtcaatatca aaaagttatt ggaggcaagg caatgaaaat ctacttaact tatatctgct
     1261 tagtttcatt gttaacaata ttattactag caatatctaa catgtatgtt gcttttagcg
     1321 tttatgcttg gctaataact ttaggatgta atttaacagg agagattaca acgtgcgaaa
     1381 acaagtgatt attacaaaaa cagtagttgg ctggtacaac attaaagata ctcaacataa
     1441 tttaatgtta aatataccgc caaaagtatt tgaacagtac tttcctgatg ttagtaaaga
     1501 tgttcaagtt gtgtgtttag aaatggattt atcaaaaatt acagaaatta aaaataagaa
     1561 aaaagtaggt agttaagatg gaaatcaaac aaaaatatca attatcaaaa gtggttaaaa
     1621 tattagaagt agtattatac gaggaagata agtttcaatc cgataaggac tatcattatc
     1681 aggataaagc attatatgaa tatgctttaa agttagttca taatggattg ttcaatattc
     1741 ttgctgaatt agattttgaa gatgaagcat ttttaattct tgatgaagta acaatgacgc
     1801 taagtgatgt catgaaagaa acacaacacg tttaccgtta tagtgtcata gatgaaaaag
     1861 gtgaacacaa acatacaaca gatcgcaaag gacacgtgat tggaatgtta gagtgggcat
     1921 tagattacat tgcgggaaat attgaagtgg aggaattata aatgaattgg gaaattaaag
     1981 atttaatgtg tgatattgaa gcggtaaaag aaaaaatcaa tgatgtagct atcaaacatg
     2041 cttggtttgt tgaagataga tttgtaaaaa atgaattaga aacaaaacgg gaacatatta
     2101 atttttctgc tagctattta gaacatcgta tacaaaatga acatacagtt gagttattac
     2161 atgtgtactt aaaagaattc ggtgaactta tacaaaaatt tcatgaaata gaaaaagcat
     2221 catctgagaa ctttggcgag gtatcagatg acgcacaaaa attaaaaatc acagagtaat
     2281 ttagaaatta cacatgttta ttataacatt ttttactctg tgaatcacta gaggtgcaaa
     2341 aaatgaatga aattaaatta gaatatgaca cacatgtttc agtggtacat tatgaaagtt
     2401 tagactcacg ttcatttaat agcttttcaa aaattaattg gagtaagttg gttaataaac
     2461 tgtctgtacc tatagaagca aattataagt atgcacgtgg tgttgctgtt tacggtgata
     2521 ttaaaaacgg tgcaaatgat caaggtgaaa ttatcaaaaa gcatcgaaac gataaaaatg
     2581 tcatatacag agatgtgatt gtacttgatt atgatgaaat aaatgattta aagcaattac
     2641 atgaagcaat cagctcagct ttaagcaatg ttgcatggtt ttggcacaca agttactcgc
     2701 acagaactga acaagctaga atacgcctgt atatccctct aaatgagcga ataagtgcag
     2761 atgattatcg taaatataca aaagtattag caaataaaat tggccataaa gtggatgaag
     2821 gttcatatca gccaagtaga tgttttgcgt taccagttat tcaaaaagga cacatattta
     2881 ttaagcgagt gaatgactgt ccaattatga atgttgatat gctcgaacag tggtcgaagg
     2941 agtttgaaca atcaaatgct agtcctaatg tcataggata cactcgacgc gatagtgagt
     3001 actggcgcga gctatgcttt ggaacaaccg aaggcaatcg taacaatgca ctagctagct
     3061 taattgggca tttattaaga tgtcacgtta atgattatat tgtttattca tttgctttac
     3121 tatgggggca attcgcatgt aaaccaccta tgaaagaaca agaaatcaac gccacttttc
     3181 aatcgatatt aaataaacac tataacaatt agaaaggggc tttgtatgga aacaggtaaa
     3241 agtgatgtac ttgataaaat tgaaaaaatt aataaaaaag atagtgcctt acaagaaatt
     3301 ataccaaaag gttatgaaat tgaacatcat caatgcggta ttgccttaaa tcaacttata
     3361 ccaagtaaaa aagaaggcga gccagataaa aaggttttta tcacaagtac aatccctcaa
     3421 atcactgaac gctttgaaga tattgagagt aacgaagtca gctttaatat gcttttctat
     3481 gacaataaaa cgccagtaaa tatagctgtg agtgccgaag aaatttcaga tagtcgtcaa
     3541 ctcttgaaat tggttaataa aaagctggat gtaacatcgt cgacatctac taaacttgtt
     3601 gattatatta atgcatctaa acggtataat ccaccattga atgttaaagt tgcaacgcgt
     3661 ttggggcatg tgaaaggtta ttttatttat ccttatcaag aagtgatgaa agacagcaat
     3721 atcaagttgt ttagtaatga taaaggattt caaaagttaa tagactcttt tcaaagcaaa
     3781 ggaacattag aaggttactc taaaaaagtg ttcggtcaaa taaaagatct accaatggta
     3841 atggttatgt tatatgcctc tttaggttcg gttttattaa gagaatttgg attacagccc
     3901 tttattgtag aaatatcagg tagtacatct acaggtaaaa cattcacact caacttagta
     3961 tcaagtgttt ggggaacgag cgaccttatc acgacatgga gttctactca aaatagtatt
     4021 gaatcgatgg catcattttt gaactcattt ccaatgttta aagatgatac acgtaataca
     4081 catcctaagt ttgttaccag tgccacatat aacttttcta gtggtgaaag taaatcaaga
     4141 agtaatatta atttaacact aaacgctaaa aaagaatggc gaaatatttt aatttctact
     4201 ggtgaatcat ctatcgcaaa tatggctgat gaaaaagcgg gtgtatcagc acgtgtagtt
     4261 acactacaag atccaccata tccagataat tttgatttta ccacattaga caaatcgttt
     4321 agggagaact atggaacgtt agggttggca tttattaaac aatatgagtc taaaaaagac
     4381 gtgtataaga acgcttttga gagctatcaa cggtatttta atcaaaaagg tagtaatgaa
     4441 atcatgcaac gtttaggacg tgcctttgcg ttactacaag ttaccggtga ggttttgaat
     4501 gatattgatg ggtttgaaca tgaccatttt aaaattatcg aacaagccta tgacagcatg
     4561 gttaaaaaca ataagacgat tgataaacct aagcaactgt tagaggaact attacaatat
     4621 ttagatgcaa atagaaataa tatcgctggt gatggctata gttcagtcaa aaatggtgac
     4681 atcaaagcta tatataaacg tgattattta tgtatattag gtcaaactgt acacgataaa
     4741 ttaggtcatg aaatgcagac tataacaggt caatggggca aaaaaggata tttaattaaa
     4801 ggtgaaaaag atcgcttgca aaaaaaggtg agtcacaaaa acattaagta tagaggattt
     4861 gctataaaca aagaaatgct tgaagaatta ggatttgatt tctcgaattc tcataatcct
     4921 tattcagatt attaaatagt tcccaaagtt cccgataagt tcccgcgaaa aacatacaaa
     4981 cgggaactat aagactactt taaccacaag caattaaagt taatagttcc cgaagttccc
     5041 aataaataat attattattt attatttgaa aacgaacaaa tgttgttagc tttataccat
     5101 atatgataga aaatttttaa cgggtacaac gggaactaag tttatttaaa gtttatatat
     5161 caatggtttg actagttccc gataagtatt ttaagtcggg aattcaacgg ggactagttc
     5221 ccatttaaaa atattggagg taacacatgg ataaagagca acttaaaaag tatatatacg
     5281 attatgtaaa agaatataag gagataccga tatatcagtt agaagatttg tttaaagaaa
     5341 tgaatcacga ctatataggg agaaccagtg tcacacacga taaggatgag aatattgtgt
     5401 tttggagtgg atggaacaaa attacaatgt ttgcgctgat tgaattagtt aaaagtgaac
     5461 aacttgattt agtgtataga ggtagttttg taatgcgtta tttgttggat ggtagagttc
     5521 ctaacttacc attagcaatt tgttatccag aagatggaca acaaacggac gtgccctcat
     5581 gggtgcctat ggtattaaga ataaataaag aggagaaaat caaatgaaca tagaaactat
     5641 cgtaaaccaa tttgaaacac gagcaggcac gttactaagg tactacacag gattattaga
     5701 acatagtaaa gtgcaaccat gttgctttaa gttatacaat gatccatttg atatggcata
     5761 cgtgatgatg aatgggaagt tattcggtca tgtatatatt aaagattgta aagtaaggca
     5821 atcatttgaa ttagcgtcac ctaagcacac tgaggggctt ataagaagta tagaaggtca
     5881 ttatgtaggt tatgaattac atgacggtaa acagctttct attagtgata tgatggccag
     5941 tcaattattt gaagatgagt attttatgta tggattacaa acatatgcag aatcaaataa
     6001 tagtgatgtg tttgagtacc tagaaaatgg atttgatacc gatacacttg agggcattca
     6061 atcgagtaat actgatgtga tagcgaatat tgaaatgttg tatcagttag ctacaggaat
     6121 caatgaacca gcaccagagt tagttgaggg gttgagatta gtaactgagt ttgtacaaga
     6181 tgagaatgcg acacaagagg attacaaggc tttagagcat aagttaactg agttgaagtc
     6241 atcttattac agtttgaata agtaattaaa tatggagtct cacgtggtgt gtggctccta
     6301 atataaaagt ataaggtata gaagttttaa aatgtaaagg ttgcaacaat agtgagttaa
     6361 tagataggta ggcgaaattc aaaaaagtgt gaaatgttga tattgagctg ttttatggct
     6421 ttgaaaataa taaggttata taaaggtgtt agcttttaac atcggaaggt atacagtctt
     6481 tgagaattga aaaaatggca agatttgtgc aaggtgtgag aactttgtta acgctaatac
     6541 aagctaaagt ttgtgttttt ggcataggcc taaaagttaa gtttgttcgc tgtttgttcg
     6601 tgttatttta tcgaacttaa gttctatatt aggttaatgt gaaaagccta acgttaagtt
     6661 tataacatga ttttataagt gttatatatg ataagctaaa caattgataa aacgcgctat
     6721 aaagcgaacg taagtttgtt ttagacctgt aaaaatggta taatttaggt atgaaataat
     6781 taaaagaaag aggtgtagaa atgcaaagta tcgcagaaaa agagacgtat catttaccca
     6841 ccgaacacct gcaagttttc aatgtgataa aaaatacgtc caataagtat attactaaaa
     6901 ctaaaatctt aaatcaattg ggatatgaat ataattcaag caatgaacga tggttacgaa
     6961 gagtaatcaa ttcattagta tatgattatg gctatcctat cgggtgcagt tataaaccta
     7021 gtgaacgtgg ttattacatc attatgacag aacaagaaaa gcaacaagcg atgagaagta
     7081 ttaagaaatt agctgatggc agtatgaaac gctatgaagc tttgaaacga attaaagtgt
     7141 aaaggggata aaaatgaaaa ctgaatcgta ctttaaagaa tacaaccaat ttgtaataga
     7201 tcaacaaaag gctatacaag aattgaaaca agagcgtaat gcattggaga gtaaaataaa
     7261 gatagataag tccacatata aacagttaat catggatgga caagatgata aagcagataa
     7321 cctatatcaa gcaacagatg ctgatgaaaa gaaactaaaa gcacttaata aacgcttaga
     7381 gacaaagaaa agtgtgtcga aagaagttaa atatcaaaag acaattgaat tattaaaaca
     7441 tcaaagcgag ttgtcatcat tatatgaatc agaaaaacaa tcagctttag gtaaattaaa
     7501 aaaggtagtc gatgcatata atgagatcat tgatgaaata gaagatatta atgatagata
     7561 tgaagatgag catcagcaat atgcgagtat ttatagtcaa gaacaattat atgatgataa
     7621 agaggctagg gaagcattga atggctactt tagagaaaat atatttacat catatattaa
     7681 tggtaatgat ttgccatacg aacacaataa caagttgttt ttaaaacgtt aaaaagaaag
     7741 ggtaattaaa tggaaacaaa atacgagtta aataatacta aaaaggtcgc aaatgcattt
     7801 ggtttaaatg aagaagatac aaatctatta ataaatgcag ttgatttgga tattaaaaac
     7861 aatatgcagg agatttcaag tgagttacaa caatcagaac agtctaagca aaagcaatat
     7921 ggtacaacgc tacaaaattt agctaagcaa aacaggatta ttaaatagca atgattgcct
     7981 atccaattcg ggtaggctct gtttataggg gtgaataaat gaaactgctt aaaacgaaga
     8041 attgtttata ttatcgtaat ggcgacaata aactatctga gtatcaacta ttaacgcaat
     8101 ttaacccagc atttattaat aaaaaaatta agatgtgtga attccaaatt gaaagtatgt
     8161 accatatgag tgcgtcgacc acaacatgtg atgaaataat gggggtcgtg tctgtctcat
     8221 atccgattga aaaattagtt atcaaaatta ttgaaacaaa agcagggtta caaaactata
     8281 aaaatagatc tataaataat atggcgttgt tgaaaaaggt actaaatcat tatacagaaa
     8341 aagagcagaa gcaagttgta aaatatatgc gttcaaatgg acgatataag ccttacaacg
     8401 tcattgaacg cttacaagtt gatttgtatc aagcaagtat taaacaacgt tcagaacgtc
     8461 aaaaacaaag aaatacagca attgaaaaca gtaagattgc acgagtaaat gcatatcacc
     8521 aatcttcata tgtaaaagtg gtgtaacaat ggataaaaag caaataaaag acttcgtttg
     8581 tgattatcat aagcgaacta gaagtgatgt gttgatagat gatgaaataa ataccgatga
     8641 attcttttca ataggtgatg aaaattctaa tgaatggatg gcagacgata acattgatga
     8701 tcatattgta aagaatcact tagaaatgat tgttgaccaa gtagctaatg acaaagagtt
     8761 ttatattttc gattctttaa tacaaggacg tagttttaaa gatattagca atgtcttaga
     8821 gtgttcagaa caatctgtaa gattatggta tgaaacctta ttagataaaa ttgtggaggt
     8881 gatagaatga gtgagttaac ggcaaaacaa gcgcgttttg tgaatgagta tataagaaca
     8941 cttaatgtaa cacaaagtgc cataaaagca ggctatagcg caaatagtgc acatgtgaca
     9001 ggatgtaggt tattaaagaa gccacacatc aagcaatata tacaagaaca aaaagataag
     9061 attatagatg agaatgtatt aaccgcaaaa gagttactac atgtgcttac gaatgcggca
     9121 gtcggtgatg agacagaaac gaaagaagtt gtagtcaagc gaggggaata taaagagaat
     9181 ccacaaagtg gcaaagtaca gctagtctat aacgaacatg ttgaactgat agaggtacca
     9241 ataaaaccta gtgatcgttt aaaagctcgt gatatgttgg gtaaatacca taagttattt
     9301 acagataagc atgatattaa cgggaatgtg cctatattca ttaatattgg tgaatgggat
     9361 ggcgatgatg aagatttaga taagacggta caagaggtat ctaacgctaa tcctaatcat
     9421 actgtgattg tggatgatat accgttagag gattgattac agtaaaaacg attatcatat
     9481 tgagttagtg aggattagtt tactaattca ccctagcttt atattaaagc gttataaaga
     9541 taaaagggag aacgcttatt ataattaacg gactcccttt attaataatt attacagaaa
     9601 aagtggtaaa ttaattaatt tctgcttcta tagtttttat ttcatcaata tttataggtg
     9661 gtttttcagt attgtattca aactttttag ataaatcact ttgatatgtg gatccgtcat
     9721 tcattgttat tttccaataa ccacccgttt tatcgcttga acgatataat ccatgtattt
     9781 gagttagctg atgacgaatt tcaaagtcta aagttgatat agctaattgt tttttatcga
     9841 actttggcca atactttaag gggctatctt taccatgaac cttaactttt aaaggtagtt
     9901 ctattggagt aggtaatttt tcagtatttg taacgccact tatttggaaa tggatataag
     9961 ttccttcgct agtatgttgg ctttttttag ttctttttgt gtttaagtca actttttccc
    10021 cttttgtaaa agcagggcta taataaggac tcggaaaaat tataaggctg atgctgccat
    10081 ctgtgttttt tatacgcata gatcctaagg aattatctaa aacttcacta tttgtaaaag
    10141 tgtcagaccc actactatac cagtctagca aatcctttat attatcgttt gtagatgctt
    10201 ttgcagtttt gattatttga ttagataata agggaacagg ggtaaaatct gtagcgattg
    10261 tcgcaagcaa caaagggctt acgataaaaa aattcattag taatttttta ttcattttta
    10321 attctccttc attcaaatgt gtaaacgttt acatatagaa tgtataaata ttatttaaat
    10381 gaatcaatta accatctcta aattattgtt taaatatata ttaattaaaa agtgtttgtt
    10441 acatagggag ctatatcaaa aaatatagat ttaaacaaca ttttaaagtt acaaatagca
    10501 aaaaacaaag tgtgagtggt catttaaaga atattaatta atataagttt gaattagtta
    10561 tattctttaa agtcactttc tagtggcgtt ttttacgctg agaaacgtcc tgtgttgcag
    10621 taagggatgg gaaccacgca caaatacttt aactgtaaac ataacgtcgt gaaatatggc
    10681 tttaaacatc gctggtcaat ctatctttga gattggtcga agattaaaac catatgaaac
    10741 aaaatgacct agcacatgga gaatttggta attggttgaa aaatatcaac ttagatagaa
    10801 cacaagctca tcgtttcatt aaagtttctg aagaaattaa agatgttggt acataccaac
    10861 atttaggtct gagagcttta tcggaaatag ctagcttacc tgtaccagta cgcatcaaag
    10921 tacacataac atcaaacggc aaaactaaaa ttccatacta aatgtttatg aatttaactg
    10981 cagaactcaa ttttgagcct tgtaaaatta cataaattag ttatataaat attatttatg
    11041 ggtattatat aaacgggagg ggcaacgtta ttacttgcct attagaacat ggaatggttc
    11101 tgccccaact agtcaggtac taggcgacta atggggagaa atcagttgaa atgacatagt
    11161 catgtctatt taagcaggtg tgtaacacac ctgctttcta tttacattta aagataaaat
    11221 gtgctattat tttactagaa ctttttaaca tttctctcaa gatttaaatg tgcataacag
    11281 gcaggtactt cggtacttgc ctatttttat gtaaacatat taggtgtatg tataaattta
    11341 gggtattgat tatagatact taatatatgg cggaatggtt gaaaaatgat aaagtgagga
    11401 aagtgtttga ctttcattag tattagtact tttaatgatt ttatttttaa t
//
LOCUS       DATTQF010000081        11391 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.081, whole genome shotgun sequence.
ACCESSION   DATTQF010000081 DATTQF010000000
VERSION     DATTQF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11391
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.081"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..97
                     /locus_tag="VB241_001764"
     CDS             <1..97
                     /locus_tag="VB241_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEP1245056.1"
                     /translation="LMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(100..489)
                     /locus_tag="VB241_001765"
     CDS             complement(100..489)
                     /locus_tag="VB241_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245057.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            559..1101
                     /gene="rsmD"
                     /locus_tag="VB241_001766"
     CDS             559..1101
                     /gene="rsmD"
                     /locus_tag="VB241_001766"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP1245058.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGIDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            1103..1585
                     /gene="coaD"
                     /locus_tag="VB241_001767"
     CDS             1103..1585
                     /gene="coaD"
                     /locus_tag="VB241_001767"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP1245059.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(1647..2786)
                     /locus_tag="VB241_001768"
     CDS             complement(1647..2786)
                     /locus_tag="VB241_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP1245060.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNNIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHKFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSYINEATDFHHFV
                     KSLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTVFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            2913..3470
                     /locus_tag="VB241_001769"
     CDS             2913..3470
                     /locus_tag="VB241_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP1245061.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3550..3723
                     /gene="rpmF"
                     /locus_tag="VB241_001770"
     CDS             3550..3723
                     /gene="rpmF"
                     /locus_tag="VB241_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP1245062.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(3877..5835)
                     /gene="isdB"
                     /locus_tag="VB241_001771"
     CDS             complement(3877..5835)
                     /gene="isdB"
                     /locus_tag="VB241_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HEP1245063.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAKAAEET
                     GVTNTEAQPKTEAVASPTTTTTEKAPVAKPVAKPVANAVSVSNKEVVAPTTETKEAKE
                     VKAVKEVKAPKEAKEEKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDF
                     EMKKKDGTQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKL
                     VSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKT
                     EEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEETKKALDEQVKS
                     AITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVME
                     TTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQ
                     YHVRIVDKEAFTKANADKTNKKEQQDNSAKKETTPAMPSKPTTPPVEKESQKQDSQKD
                     DNKQSPSVEKENDASSESGKDKMPVTKPAKAEVESSSTTPTKVVSTTQNVAKPTTASS
                     ETTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEES
                     NKDMTLPLMALIALSSIVAFVLPRKRKN"
     gene            complement(6037..7101)
                     /gene="isdA"
                     /locus_tag="VB241_001772"
     CDS             complement(6037..7101)
                     /gene="isdA"
                     /locus_tag="VB241_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HEP1245064.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQAVLNNASFWKEYKFYNANNQELATTVVNDDKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPAVTAPSKNENRQTTKVVSSEATKDQSQTQSARTVKTTQTAQDQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKQNEVHKQGPSKDSKAKELPKTGLTSVDNFISTVAF
                     ATLALLGSLSLLLFKRKESK"
     gene            7310..7993
                     /gene="isdC"
                     /locus_tag="VB241_001773"
     CDS             7310..7993
                     /gene="isdC"
                     /locus_tag="VB241_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HEP1245065.1"
                     /translation="MKNILKVFNTMILALIIIIATFSNTANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKERIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPSDVAGANAPGKDDKN
                     SASGSDKGSDGATTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            7993..9069
                     /gene="isdD"
                     /locus_tag="VB241_001774"
     CDS             7993..9069
                     /gene="isdD"
                     /locus_tag="VB241_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HEP1245066.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTSSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KDLDNKEMLYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     VGIVLATILLISAHLYSRKRGNQV"
     gene            9066..9944
                     /gene="isdE"
                     /locus_tag="VB241_001775"
     CDS             9066..9944
                     /gene="isdE"
                     /locus_tag="VB241_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEP1245067.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            9957..10922
                     /gene="isdF"
                     /locus_tag="VB241_001776"
     CDS             9957..10922
                     /gene="isdF"
                     /locus_tag="VB241_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HEP1245068.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            10984..>11391
                     /gene="srtB"
                     /locus_tag="VB241_001777"
     CDS             10984..>11391
                     /gene="srtB"
                     /locus_tag="VB241_001777"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEP1245069.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKILNHNTILYGHHVGDNT"
BASE COUNT         3796 a         1563 c         1995 g         4037 t
ORIGIN      
        1 actaatgcaa cttaaatacg ttagacacat tgatggctca ccatataaat acttaaagaa
       61 aacttacgaa aaagagaaac acgaaatata taattaatat tattgaagtt ctggtatcca
      121 tttttggagc cttaacacac taattaaata attataatat aacgataaat catgaaatgc
      181 ttgaggaggc tgtacatcga tacctaaaat tggtatgtcg aatttttcag cgcaatcatt
      241 aatcatttga aactttttat ttgaatctgg atatggatat ttaatagcgt taatcatgat
      301 ataaaatgct tggaaatgat tgccagttgt agggttcata atgattgcta cagatgactt
      361 tttattttca gcttgtggtg aatgaaaata gataatttta tcaatacgtt gatggttata
      421 ttcaatcatc gtcatgaatt cgaacaaatc tgttagacct tcaccaagtg taataaatgt
      481 ttgtttcatt ggtttatcat tctccttttt ctcaataaat tatattaaat aactaaatga
      541 attggaagtg tgaacaacat gcgcgtcatt gcaggtaaac ataaaagtaa agctttagaa
      601 agtatggaag gccgtaatac gagaccaact atggataaag ttaaagaagg tatctttaat
      661 agtttatatg atgtgtcagg tataggttta gatttatttg caggaagcgg ggcgcttgga
      721 atagaagcac tctctcgagg tatagataag gttatctttg ttgatcaaaa ttttaaagct
      781 gtaaaagtta ttaaatcaaa tcttgcgaat ttggatttag aggcacaatc tgaagtttat
      841 aaaaataatg cagatagagc tttaaaagca ttgtcaaaac gtgatattca atttgatgtc
      901 attttcttag acccacctta taataaaggt ctcattgata aagctttaaa actaatttca
      961 gagtttaatt tattgaaaga aaatggtatc atcgtttgtg aatttagcaa tcatgaagaa
     1021 atagactatc aaccgtttaa tatgattaaa cgttaccatt atgggttgac agacacattg
     1081 ttattagaaa agggagaata gcatggaaca tacaatagcg gtcattccgg gtagttttga
     1141 ccccattact tatggtcatt tagacattat tgagagaagt acagatagat ttgatgaaat
     1201 tcatgtctgt gttcttaaaa atagtaaaaa agaaggtacg tttagtttag aagagcgtat
     1261 ggatttaatt gaacaatctg ttaaacattt acctaatgtc aaggttcatc aatttagtgg
     1321 tttactagtc gattattgtg aacaagtagg agctaaaaca atcatacgtg gtttaagagc
     1381 ggtcagtgat tttgaatatg aattacgctt aacttcaatg aataaaaagt tgaacaatga
     1441 aattgaaacg ttatatatga tgtctagtac taattattca tttataagtt caagtattgt
     1501 taaagaagtt gcagcttatc gagcagatat ttctgaattc gttccacctt atgttgaaaa
     1561 ggcattgaag aagaaattta agtaataaaa ataacagtat tttaggttta tcatggttta
     1621 caatcctaaa atactgtttt catttgttaa cgatattgct gtatgacagg cgtgttgaaa
     1681 tctgtttgtt gttgcccgct tattgcattg tatatgtgtg ttgctttgat ttcatttgtg
     1741 aagtaatgtg cattgctttt gttaatattg gttatatatt gtctttctgg gaacactgtt
     1801 tttaaatgct ttaaatattg tctgccacgg tcgttcattg ctaatacttt aactgcgtga
     1861 atgtttctcg taacatctgt aggtttaatg tttaataata cattcattaa cagtctttgg
     1921 atatgcgtat atgtataacg ctttgttttt agtgatttta caaaatgatg aaaatcagtt
     1981 gcttcattaa tgtaagattt taaacgattt tcaaaacctt cagtaacagt ataaatattc
     2041 tttaatgact ctgtagtcat agctatgatt tgatatttca aatatgggaa tatttgattt
     2101 aatgttatat gaggtgttac gtacaggtgt tgaatatctt taggtaccac atgatgccaa
     2161 tgatcatctt gactaatgat tgatgttcta atagatgtac cacttgcaaa cttatgatgt
     2221 tgaattaatg aatcatgatg ttgagcattt tctcgcttaa tagaaattgc attgatgttt
     2281 ttagcatgtt tagcaattgc tttcagataa ctaataccaa gtatgttgtt aggacttgct
     2341 agtgcttcat gatgctctaa taattcgcta atgatacgag ggtagctttt accttctttt
     2401 actttttgtg aaaaggattc ggattgttca atttcattaa tgctgtgagc taattgcttt
     2461 aatgttttga tattattatt ttcactacca aatgcaattg tatcgacact catataatct
     2521 gcgactttaa ctgctagttc ggcaaaatga tcgccagatg ataaactggc agttgctggt
     2581 agttcgataa ctaaatcagc tgttgataat gccatttttg cacgagtaaa cttattatag
     2641 attgctggtt cgccacgcat gacaaagtta ccactcatta ttgcaatagt aacgtcagca
     2701 tttgtaagtt ttttagattg attaatatga tattgatgcc cattatgaaa gggattatat
     2761 tctgtgatta agccaacgct tttcatttgc aattcgtcct ttcggtattc attattaata
     2821 ttgtaacaga acatgatatg ttaagaaaaa atcttgacaa cttgttctta gaaagttaaa
     2881 ataaattttg tgcttgttag aggtgaagcc atatgaaatg gtcaattacg caattaagga
     2941 aatatcaagg taagccattt gaatttgatc aaacggtgag ttttgacaat ttaaaagaat
     3001 cattagattt aattgattta tctccaatta caatccaagg tcagttaacc attaagtcaa
     3061 cagaagtcgt tgcggatatt cacattactg gaacgtatac aatgccttgt gcacgtactc
     3121 ttgtaccagt taaagtccca ctagatgtaa ctactacaga agtatttgat ttagaagggt
     3181 acaatcagta taacgatgat caagatgatg tagatgaaca ctatcacatt attaaagatg
     3241 gtatggttaa tcttcaggat attgtcgagg atatagttat tattgagaaa ccaatgagag
     3301 cttattcaga gcaaagtgac caaatgttga cagtaggtaa tggttgggaa gtaatcgatg
     3361 aggatcaatt agatgagctt gctaaacagc aagaacaaga tgattcagaa tcacgacaag
     3421 ttgatccaag gcttcaaaaa ttacaacaat tatatgataa agagcaataa gtagtttaat
     3481 taatgatata atgttgttaa ttaaactaac atttagtatt tttcgtttat aatctaagga
     3541 ggatatatca tggcagtacc aaaaagaaga acttctaaaa ctagaaaaaa caaacgtcgt
     3601 acgcatttca aaatttcagt accaggtatg actgaatgcc caaactgtgg cgaatacaaa
     3661 ttatcacacc gtgtatgtaa aaactgtggt tcttacaatg gcgaagaagt agcagctaaa
     3721 taattttagt tactcaatat aaaaagtccc gcttaaaata attgttttaa gtgggacttt
     3781 ttatattgca aaaaataatt ggcggacgag gtatcggaat acctcatctg ccaattaaaa
     3841 tttgttaatt taataattaa atataaagac gattaattag tttttacgtt ttctaggtaa
     3901 tacgaatgca acgatactac ttaaagctat taatgccatt aatggtaatg tcatatcttt
     3961 attagattct tcaccagttt gtggtaatga tttagcttta ttttcttgtg tatttttatt
     4021 attttggctt tgagtgtgtc catcatttgt gtttttaatg tttgcttttt gtaatggagc
     4081 actatctttt gcttcgctag aacctgctga agtttgaaca acatcttttg ttgtttctga
     4141 agaagcagtt gttggttttg caacattttg agtcgtagat actaccttag ttggagttgt
     4201 actacttgat tctacttcag ctttagctgg ttttgtaaca ggcattttgt ctttacctga
     4261 ctcactagat gcgtcatttt ctttttcaac acttggtgat tgtttattgt catctttttg
     4321 gctgtcttgt ttttgtgatt ctttttcaac aggtggtgtt gttggtttgc taggcatagc
     4381 tggagtagtt tccttcttag ctgagttgtc ttgttgttcc tttttattag ttttatcggc
     4441 attggcttta gtaaatgctt ctttatcaac gattctgaca tggtattgtc catcataatc
     4501 aatcgttttt acgtgaactt taacgatagc atcatataga gttttacctt caacatatgg
     4561 gaagataatc gttctagtgt tatttttagc atctttgcta attgttctaa cacgttgacc
     4621 ttcaaccatg aaatctttcc agtaatcgtc attagtagtt tccattacca tatatttttt
     4681 gccattaagc atacctgttt taatagggtg tttaacaaaa gtatccatca tagattcgtt
     4741 attctcaaca ctttcataaa caacatattt tgtatcttgt aaatcggtca ttttttcatt
     4801 tgttggttgt acattttgga attcagttat cgcggatttc acttgttcat ctaaagcttt
     4861 tttcgtctct tctaatttct tcttgtattc agctttcaat ttttcaggaa gtttatcttg
     4921 aattttattt aattcataaa cttgtctttc tagtgttttc gcttttttat atggcgctaa
     4981 taatttttca gctttataat cttcttcagt tttgaattta tctgcactgt tataaattgg
     5041 ttgtgcgaat tccattaatg tgtaatcgta tttttcttct ttgttattga agtgagttga
     5101 acttacgatt ttaactgctt ttgttccatt tgaaacagag aagcgaatgt aagcataatc
     5161 tttaacagta tcgtatgata ctaatttaat tggcaacttt ttgtcacctt cataaacttc
     5221 aaattttctc caaaattgcc ctgattgtaa tcctaattca atttctggtt ttgaatcagt
     5281 gaaaataact ctagcaggtt taacagaact tgcataatga taaaactgtt gagtgccatc
     5341 tttctttttc atttcaaaat caattggacg agagtttggt gcgctatgat ctttgtcttt
     5401 tattgcaggg tttttaatcg cttctcttag ttcctgattc aaaataggat atgtattgtt
     5461 gtcagctttt gctgcaggtt tttcctcttt tgcttcctta ggggctttaa cttctttaac
     5521 tgctttaact tctttagctt cttttgtttc agtagtaggg gccacaactt ctttattaga
     5581 tactgagaca gcattagcta ctggtttagc tactggttta gctactggag ctttttcagt
     5641 tgttgttgtt gttggacttg caactgcttc agtttttggt tgtgcttctg tatttgtaac
     5701 acctgtttct tcagctgctt ttgcttcgcc attggacatt aataataaaa gtgtactaat
     5761 cgctacagat gcaacgccta gtgatgactt tctaattgaa taaaatgatt taaattcttt
     5821 ttgctgtttg ttcatgttgt agaaacaact cctaattgta tattatcaac tgataatcat
     5881 tatcaattga ttacttgtat tgtacattga aatggtagtt aattgcaatg ttattttata
     5941 aaaaataacc tatgttatag attatgtaat atctatgaca taggttaaat aaaatttaaa
     6001 ataaaaaata tattaagtta tgagtatgac gaagatttat ttagattctt ttcttttgaa
     6061 aagtaacaaa gataatgaac ctaaaagggc aagtgttgcg aaggcaactg tgctaataaa
     6121 gttatcaact gaagttaaac cagtttttgg taattcttta gctttagaat cttttgaagg
     6181 tccttgttta tgaacttcgt tttgttttgt aactttgtta gtttgttgtg atttattgtc
     6241 acttacagct tgattgttgc tttcagattt cgctgttgca acatctttaa caggtgtttg
     6301 aactttattt tgatcttgag ctgtttgtgt tgttttcact gtacgagcac tttgtgtttg
     6361 actttgatct tttgtagctt cactacttac aacttttgta gtttgtctgt tttcattttt
     6421 gcttggtgca gttacagcag gtttaacttt ttcaacttta ggttgaactg gtttcgtttg
     6481 ctcagtaggt gttttaggtt gagcaggttt tggttgaacc ggtttaacat tgtttggttt
     6541 tgctgcgtca gctaatgtag gaattgcttt ttcaaattct aaatgcgtag tatatctatg
     6601 attataatta atttgtggca cgacaatatg tacttttgta gttaaactct tatacccagg
     6661 ttcaacagca acattgattg ttctagtgtc agctttttta tcatcgttaa caacagttgt
     6721 tgctaattct tgattgtttg cattgtaaaa tttgtattct ttccagaatg atgcgttgtt
     6781 caatacagct tggaaataat atttattatt ttgtttaatc actttaccag ggtgttgcat
     6841 atagtcatcc atgtgtgact tctctgaaga gccatctttt tgcacttgga aattaattgg
     6901 ttgtgatgtt gcttgagaaa cttgtgtgct ttgattatta gttgcgttcg tagcttctgt
     6961 tgccgcatta acttgttggc tgtctgcgcc tatgtataca agggaaccta aaatgataga
     7021 tgctgtaccc attgtaatct ttttcatagc tgatgaacgt tgttctgatt gatacttact
     7081 gtttaaataa tgttttgtca tgttgttttc ctcctaagga tacaaattat gtgttgttat
     7141 atgagatgat taaccattgc aattgataat ggttatcaat tatactagca catctatttt
     7201 cgtataacaa taataaattg attataaaat aaatattgac aatgataatc attattattt
     7261 atgattttag taaagactta aaagcaatca taaaaaggag gattatgttt tgaaaaatat
     7321 tttaaaagtt tttaatacaa tgattttagc gttaattatc atcatcgcga cattcagtaa
     7381 tactgcaaat gccgcagata gcggtacttt gaattatgag gtttacaaat acaataccaa
     7441 tgacacgtcg attgctaatg actattttaa taaaccggca aagtacatta agaaaaatgg
     7501 taaattgtat gttcaaataa ctgtaaacca cagtcattgg attacaggaa tgagtataga
     7561 aggacataaa gaaagaatta ttagtaaaaa tactgccaaa gatgaacgaa cttctgaatt
     7621 tgaagtaagt aagctaaacg gtaaaataga tgggaaaatt gatgtttata tcgatgaaaa
     7681 agtgaacgga aaacctttca aatatgacca tcattacaat attacatata aatttaatgg
     7741 accatctgat gtagcaggtg ctaatgcacc aggtaaagat gataaaaatt ctgcttcagg
     7801 tagtgacaaa ggatctgatg gagcgactac tggtcaaagt gaatctaaca gttcgaataa
     7861 agacaaagta gaaaatccac aaacaaatgc tggtacacct gcatatatat atgcaatacc
     7921 agttgcatcc ttagcattat taatcgcaat cacattgttt gttagaaaaa aatctaaagg
     7981 caatgtggaa taatgagaaa tgttaaacaa attgctacaa aatctattat agctattatt
     8041 agcttaggta ttcttacata tacaacaatg attggtagcg tgttggctga tgagataaaa
     8101 tatccatcag ccaaatttaa tcaacctgaa gcaaaagata aaacagaatt aacttcatca
     8161 atttttgatg aaaagataaa agagaataaa gcgttagaat tactaatttt taatcaagaa
     8221 aataaaaatg taactgagga acaacaacta gttgacgaaa aggcgcaatt gatttcagat
     8281 atgactggta aaatttactt gcaagtaaaa ctaaaaggtc aaattgataa agaacaactt
     8341 gtttttcaaa atgacaaaaa tgaagaattc ccctttgtta taaaagatga aaaggatgac
     8401 acaatagtaa gaattttaat tgaacagcat atggataaaa tcaatatgca tgttaaaacg
     8461 ttggctgaaa agaaagatct agataacaaa gaaatgctgt attctattca ttttaaagag
     8521 aaaaaagtac aacatgacga tgcaaaagaa gtgccttcaa aacatcaaaa tcaagaaaat
     8581 aatcaagatc agcttaaaaa agatattgat gacaaaaaag atagtcaaaa atcagacatt
     8641 aaggaaagac gtactagcct ttttactgaa aaaggattaa atgatattcc tgtacaaaaa
     8701 gataaagtgc aacaagacag taataaaaag attgaaaacg agcgacctaa agcatcaggt
     8761 acattaaaag ttgaaaatag ccctccaaca gtaaaaaagg ttgaaaataa tcacaaagag
     8821 caaccgaaac ataaagatga aaaatcaaaa aaggaaaaga aaaaagtagt tgaaaaagaa
     8881 aaagcgttac cagcttttaa tagagatgat gatagcaaga atagtagtca attatctagt
     8941 gatattaaag aacttgatga accaaatcat aaaaagcaat atatgttatt tgcagttggc
     9001 attgtgttag caaccatttt acttatttcg gcacatttat acagcagaaa gagaggtaac
     9061 caagtttgag aatcataaag tatttaacca ttttagtgat aagcgtcgtt atcttaacca
     9121 gctgtcaatc ttccagttct caagaatcaa ctaaatccgg cgaattcaga atcgtaccaa
     9181 caactgttgc attgacaatg acattggaca aattggattt accaattgtc ggcaaaccca
     9241 cgtcatataa gacattgcct aatcgttata aagatgtacc ggaaattggt caaccaatgg
     9301 agccgaatgt tgaagctgtt aaaaagttaa aaccaacaca tgttttgagt gtgtcaacga
     9361 ttaaagatga aatgcaacca ttttacaaac aattaaatat gaaaggctac ttttatgatt
     9421 ttgatagttt aaaagggatg caaaagtcga ttacacaatt aggtgatcaa tttaatcgta
     9481 aagcacaagc aaaagaatta aatgaccatt taaattctgt aaagcaaaaa attgaaaata
     9541 aagcagctaa acaaaagaaa catcccaaag tattaatatt aatgggtgta cctggtagct
     9601 atttagtagc aactgataaa tcatatattg gtgatttagt taaaatagca ggtggagaaa
     9661 atgttattaa agtgaaagat cgtcaatata tttcgtctaa tactgaaaat ttgttgaata
     9721 tcaatccaga tattatttta cgattaccac acggaatgcc tgaagaagtt aagaaaatgt
     9781 ttcaaaaaga atttaaacaa aatgatattt ggaaacattt taaagctgtg aaaaataatc
     9841 atgtttatga cttagaggaa gtgccattcg gtattacagc aaatgttgat gctgataagg
     9901 caatgactca attatatgat ttattttata aggataaaaa atagtgagtt gatatgatga
     9961 taaaaaataa aaagaaacta ctatttttat gtttgttagt cattttaatc gcaacggctt
    10021 atatttcgtt tgtaaccggt acaattaaat tgtcatttaa tgacctattt acaaaattta
    10081 caactggtag caatgaagca gtggattcaa ttattgattt gcgattgcca cgtatattaa
    10141 ttgcattgat ggttggcgca atgttagcag tttctggagc attattacaa gcagcactac
    10201 aaaatccttt ggcagaggcg aatatcattg gcgtttcatc aggtgcactt ataatgagag
    10261 cactttgtat gttgtttatt ccacaattgt acttttactt accattatta agttttattg
    10321 gaggtttaat accattttta ataattatat tgttgcattc taaatttaga ttcaatgctg
    10381 ttagtatgat attagtaggt gttgcgttat tcgtattatt aaatggtgtt ttagaaattt
    10441 taactcaaaa ccctttaatg aaaattcctc aaggcttaac aatgaaaata tggagtgacg
    10501 tatacatatt agcagtatca gcattattgg gattaatatt aacattacta ttgtccccta
    10561 aattgaattt actaaattta gatgacatac aagcgcgaag tatcggtttt aatattgatc
    10621 gttacagatg gttaacaggt ttattagcag tatttttagc aagtgcaact gttgcgattg
    10681 ttggacaact agccttttta ggtattattg tgccacatgt ggttagaaag ctagttgggg
    10741 gcaattacag agtacttatt ccgttttcta cagttattgg tgcatggcta ttgttagtgg
    10801 ctgatttatt aggacgagtg atacagcctc ctttagaaat tccagccaat gctattttaa
    10861 tgattgtcgg tggtccaatg ctaatttact taatttgtca aagtcaacga aatcgaatct
    10921 aaaacggtac aacaactggt ttagtgatga ctgaagaaac gtcgttaata aaagaggatt
    10981 tttatgagaa tgaagcgatt tttaactatt gtacaaattt tattggttgt aattattatc
    11041 atttttggtt acaaaattgt tcaaacatat attgaagaca agcaagaacg cgcaaattat
    11101 gagaaattac aacaaaaatt tcaaatgttg atgagcaaac atcaagcaca tgtgagacca
    11161 caatttgaat cacttgaaaa aataaataaa gacattgttg gatggataaa attatcagga
    11221 acatcattga attatccagt actacaaggt aagacaaatc acgattattt aaatttagat
    11281 tttgagcgag aacatcgacg taaaggtagt atttttatgg attttagaaa tgaattgaag
    11341 attttaaatc ataatactat tttatacggg caccatgtcg gtgataatac g
//
LOCUS       DATTQF010000082        11070 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.082, whole genome shotgun sequence.
ACCESSION   DATTQF010000082 DATTQF010000000
VERSION     DATTQF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.082"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(68..541)
                     /locus_tag="VB241_001778"
     CDS             complement(68..541)
                     /locus_tag="VB241_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEP1245070.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            892..2667
                     /locus_tag="VB241_001779"
     CDS             892..2667
                     /locus_tag="VB241_001779"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEP1245071.1"
                     /translation="MYYSYGNYEAFARPKKPENVEKKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DHASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVKDIKVDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            2992..4653
                     /locus_tag="VB241_001780"
     CDS             2992..4653
                     /locus_tag="VB241_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEP1245072.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(5139..6527)
                     /locus_tag="VB241_001781"
     CDS             complement(5139..6527)
                     /locus_tag="VB241_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245073.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVIIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(6529..7707)
                     /locus_tag="VB241_001782"
     CDS             complement(6529..7707)
                     /locus_tag="VB241_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1245074.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMETGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFKISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            complement(7858..10095)
                     /locus_tag="VB241_001783"
     CDS             complement(7858..10095)
                     /locus_tag="VB241_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1245075.1"
                     /translation="MQRDYLIRVETESMLDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFAESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKVNYKEITFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHSDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHNSLIEMIDYFKALFPN
                     GGLYVHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHHLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGIYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDVTDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            complement(10189..10959)
                     /locus_tag="VB241_001784"
     CDS             complement(10189..10959)
                     /locus_tag="VB241_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245076.1"
                     /translation="MMKRLNKLVLGINLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQPNGKDMNVKGMVLYMNRNSRTT
                     NGYYYVDVIERQDKGIHRDNEKKYPVKMVDNKIIPTKDIKDENIKKEIENFKFFAQYG
                     SFKDLSKYKEGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
BASE COUNT         3628 a         1984 c         1752 g         3706 t
ORIGIN      
        1 atactagata ttatttataa taatctcagg aatccgcttc aaaactgcat catgacagtt
       61 tatattttta ttgagaatct ctcattttat gaattgtagg aagtaaacaa aatatgacaa
      121 gcgtcaaacc aatgataatg ataaatatca tattaaacca tagtaaattg aattgatgat
      181 ggtgttgtat ttgccaaatt tctaatactg tgaagataga catatagctc ataatctcta
      241 aatttaacgt acttaaaaat aatttaatcg ctcgaatttc acgtggtgta tgattataat
      301 gtgaccattt ggcaaatcgt tcatagtatc tacctgcaat gaaaaatata agccaaatca
      361 ctataaatgc gctattaatc aaaagcagca cccatttatc agcaaagtta tcagcatccc
      421 ctgctaaatt atagtgaata ggtactttgg ttggtaattt tgaataagtc actactgtat
      481 agcacatcat agctaagtaa ataagtagac ttaatattgt aaaagacctg attttagaca
      541 ttctatcgcc tctttcttta cattttatgt ataacactct gcctatttta ccttttaatt
      601 acattacccc aaacgattaa acaatatgta atgatactat aattgcgtca ggagtatccg
      661 cttgttaaat gtacatagct tatatttagc tgtttaacat gccacataat gattcgaatt
      721 attccaagtc ccgcacctca gaatcaaaaa acattcgagt gataaaattt taaaaatcaa
      781 aaatataaaa atgatctaat ttcgtcaaat ttaccaatat aaatactaat atttgcaatt
      841 cacaaagggg tatagtctga gtgtattcta atacgaaagg acttggtgga tatgtattac
      901 agttatggaa attatgaagc atttgcgcgc cctaaaaaac ctgaaaatgt agaaaaaaaa
      961 tccgcttact taatcggatc tggtctagct tcacttgctg cagcttgttt tttaataagg
     1021 gatggtcaaa tggaaggttc taagattcat attttagaag agttacccaa agcgggcggt
     1081 agtcttgatg gtgaaaatat gcctttaaaa ggctatgttg tccgaggtgg tcgtgaaatg
     1141 gagaaccact ttgaatgttt gtgggattta ttcagatcta tcccttcact tgaaattgat
     1201 catgcgtcag tattagatga attctattgg ttaaacaaag aagaccctaa ctattctcgc
     1261 tgtcgtgtta ttgagaaaca gggtcaacgt ttagtcacag atggagactt cactttgact
     1321 aaaacggcga ttaaagaaat tttagattta tgcttaacga atgaagaaga tttagatgat
     1381 gtcaaaataa cagatgtatt ttccgacgat ttctttaatt caaacttttg gatttactgg
     1441 aaaacgatgt ttgcatttga accgtggcat tctgcaatgg aaatgcgtcg ctatctaatg
     1501 cgattcgttc atcatattgg tggtctcgca gacttttcag ctttaaaatt cactaaatat
     1561 aatcaatatg aatccttagt attacctatg gttgaatatt taaaatcgca tggggttcaa
     1621 tttgaatacg atgtaaaagt aaaagatatt aaagtagatg ttacgacaag tcaaaaaatt
     1681 gcccgagaaa tattaattga ccgtaatggt aatgcagaat ctattaaact gactataaac
     1741 gatcttgtct ttgtgacaaa cggtagtatt acagaaagct ctacttatgg tgataatgat
     1801 acaccagcgc caccaactga cgaattaggt ggtagttgga cactatggaa aaatttagca
     1861 cgacaaagtc ctgaatttgg taatcctgat aagttttgcc aaaatattcc taaaaaaagt
     1921 tggtttgttt ctgcaacatc gacaacaaac aataaagata ttatcgatac aatagaaagc
     1981 atttgtaaac gtgaccctct tgcaggcaaa acggttactg gcggaattat tacaatcaat
     2041 gattctgcat ggcaaatcag ttttacaatc aatcgtcagc aacagtttaa agaccaacct
     2101 aaaaatgaaa tatctacgtg gatttatgcc ttatattcag atgtaaacgg cgattatatt
     2161 aaaaagccaa tcacagaatg tagtggtaat gaaatatgtc aagaatggct atatcactta
     2221 ggtgtaccaa ctgacaaaat tgaagactta gcaaaacatg catctaatac gattcctgtt
     2281 tatatgccat atattacctc ttatttcatg acgcgtgcta tcggcgacag acctttagtc
     2341 gtcccgcatc aatcacaaaa tttagcattt attggtaact ttgcagaaac agagcgagat
     2401 actgtattta caacagaata ttcggttcgt actgccatgg aagctgttta tcaattacta
     2461 aatatagatc gtggtattcc agaggtcatc aatagtacat ttgatcttcg cgtcttaatg
     2521 gatgccgtat acgaactgaa tgaccatcaa gatttgcgcg agattactaa agattcgaaa
     2581 atgcaaaaac tcgcattagc aggattcctt aaaaagataa aaggtacgta cattgagtca
     2641 ttattaaaag agcacaaatt gttataacga aaaccattaa tagattttta tttggtgatt
     2701 tcaaatcatg gagtgggata gaaatcatat gttcacaaac tttatttcat tatcccactc
     2761 acatgatttt tttgatgaca cataattgca tgattgattg catcattttg ttaaacaagt
     2821 gattacaaac ctgccatttc acactgaaaa tttacataat aagtgacgat attttacaag
     2881 tcatatacaa ataacatata ttgttaaata attttaccta atcttaacat taaatttaca
     2941 attataagcg ataatcaaaa tataaagctt atttgaggtg aaataatgga aatgtcggtt
     3001 acagaagtca ttttctcctt tttaggtggt ttaggtattt tcctttacgg cttaaaaatc
     3061 atgggagacg gacttcaagc atcagcagga gacaggctac gagatatttt aaacaaattt
     3121 acatcaaatc cagtattagg tgttattgca ggtatcgttg taactatttt aatacaaagt
     3181 agttcaggta cgacagttat cacaatcgga ctggtaacag ctggatttat gacgttaaaa
     3241 caagccattg gagtgataat gggtgctaat atcggaacaa cggtaacagc atttattatc
     3301 ggtatagatt taggcgaata tgcaatgcca attttagcat taggtgcatt tttaatcttt
     3361 ttctttaagc gctctaaaat caataacatt ggccgcatac tattcggttt cggttctcta
     3421 ttcttcggtc tagaatttat gggtgatgcc gttaaacctt tagcatcatt agatggattt
     3481 aagcaattaa tgcttgatat gtctacaaat ccaatacttg ctgtcattgt cggcgcaggg
     3541 ttaacagcac tagttcaaag ttcaagtgcg acgattggta ttttacaaga attttatcaa
     3601 caagatttaa ttagcttaaa cgcagcaatc cctgtgttac taggcgataa tattggtaca
     3661 acgattacag ctatcttagc tagtttagcc ggctcaatcg ctgcaaaacg tgcggcgctt
     3721 gtacacgtca tctttaactt aatcggggta attatcttca caattttctt gccagttgtg
     3781 attcatttga ttagtttgtt acaagatgta tggcacttaa aaccagcgat gacgattgca
     3841 gtatcacatg gtatcttcaa cataacaaat actttgattc aattaccatt tgtagcaggt
     3901 ttagcatgga tagttacaaa gcttgtccca ggtaaagata ttgctgatga ctataaacct
     3961 cagcacttaa acaaagatct tgtttatcac gcacctggtg ttgcattaca agaaactcaa
     4021 aaagaattac aaaatgtggg tcagattgtc ttatcaatgt ttgaagacat tcgcgaaatt
     4081 acaaaagacg acaaaaaatt aatcaaaaag cttgaacaaa agcatcaagc tgttgaaaca
     4141 atcaatgata gtattcgaaa ttatttagtt agaatttcta caaaagccat tacgaaggca
     4201 gacgttgagc gtttagcagt tatgtttgat gtcaatcgct ctattttaaa agtagcagag
     4261 ctaacagaag agtatgtcgc tcaattaaaa cgccaacatg atgaagatat tcgcattaca
     4321 gaagatgcac aacgcggtat ggataaatta ttcaaccatg ttgctgagtc atttgataaa
     4381 gccattgaca tgttagatgt ttatgacaaa acgaaaaaag atgaaattgt agagcgtagt
     4441 agagaatcat ttaatattga acataaacta cgcaaaggtc atattaaacg ccttaatcgt
     4501 ggtgaatgta caacaaaagg cggattacta tatatcgata tgattggtgt tcttgaacgt
     4561 atcggttatc attcacgaaa tgtttctgaa gcacttgttg gtcttaacga tgatgtacct
     4621 acagatgaag aaattgcaac aactgaaatt taatttttaa tgtcttattt atattcatat
     4681 tttttaaaat tagagattca gatgcatgta aaaagccaat ccaacattca tgggttggct
     4741 tttttgttta gcaaaattta ttatcttaaa tcggctagaa ccactgatat aataatgctt
     4801 cattagtatg cggtaagcat gacggacact gttctcggag tctgaccccg aaacgtttaa
     4861 tatacacttt tacacgtcgc cttcattgaa gcgaattgcc ataaccttca cattatatat
     4921 agttctttcc atataaatgt ccaaattttt agaacaacgc aataaataac catccaccta
     4981 acttatcaaa agtttaagtg gatggttttt tatttatatt tatattagtg ttaatccaat
     5041 catagattta tctatatgca ctgctctata catttcctca tttaatttgc tttattttca
     5101 tttaaatcat tatcaaaaca ctgggcatgt catcgttatt atttcgcatc tttgacacgt
     5161 ttatcatcat taggaatcgc aaataaaatt gcgataaatg ccatgattcc cattaatacg
     5221 ttaacccaaa gtgcaatcat cgcacctgta tgaatgctcg ttgcagcaac tgcaccggca
     5281 tatactgcac cactaattgc gacaccgaat gcaccaccaa gtgatgaagc cattttataa
     5341 atacctgaag caacgccaac tttatctaac ggtgcatttg aaatagctgt atctgtagaa
     5401 ggtgttgcat aaatacctaa gcctagtcca aaacataaat atcctacgac acaactgata
     5461 acataaaata tgcctggtaa gaatactaat gaaataagtg caataccaat gatcacaatg
     5521 aatgtaccta ataacattgg tcgcttagaa cccatttttt gtaataattt ttcaccaact
     5581 ctaatcatca ataacaccat gattaaataa gtgattgata agtatcctgc ctgcaatgct
     5641 gtataaccta aaccttgttg tacgaatgta ttcgctacaa ttaatgtacc tgcaacaccg
     5701 tttaataaga agttcgaaat cgttgcacct gtatatggtt tattttcaaa taatttaaaa
     5761 tcaataagcg gattatctac ttttttctca acatttaaga aaataaagaa tgctacaatt
     5821 acgattgcaa tcaaaccaaa gaaccataat gatgtataac caagtgctgc acctttagta
     5881 atgacaacgt ttaaacttaa taacataact actagaacaa ttagacctgc aacgtcaaat
     5941 ttatgtgtat tggtaacttc tgatttcgtt tcaggcgtcc ctttgatgag taacattgaa
     6001 agtacggcaa cgatgattga gaagatgaaa atccatctcc aacccatagt tgtcgcaact
     6061 gccccaccga agagtgaaca gataccactt ccaccccaag aaccgataga ccaatagctt
     6121 aaggcacgct gacgttcagc accctcataa taagttttca taatggccaa tgtggaaggc
     6181 ataatacacg ctgctgatac gccttgtata atacggccta aaattagcaa tgctggtaaa
     6241 tttgtaataa taattaatgc tgaaccaata atacttaata ataagccgat atttgtcatt
     6301 ttcacgcgtc caattttatc tgctaggccg cctgctccaa caacaaacat gcctgaaaat
     6361 agtgcggtta gacttaccgc aatactaatt gttcccatgt ctgcaccaaa actttgttgt
     6421 aaatttggca caacatttac aagtgattgt gcaaacaacc aaaatgtaat aacacctaat
     6481 acaataccta agattaactt gttgcccccg cgatacgttt cattcatgtt agttatctcc
     6541 tttaaggtaa tctaaaacaa ctgtccctac agcttctgcc gaaataagta atgatttttc
     6601 tgaaatctta aatttaggat gatgatgtgg gtaaatttca ccatcttcca cagctgcacc
     6661 tgtataaata aaggcacttg gtcgttcctt agcatagtat gcaaagtctt ctgaaggtgg
     6721 ttgtggttca catatttcga caccaaattc aaggtctgct tcttttagcg tcttagccac
     6781 gtactcagta aactctggat cattatataa tgcaggataa tcatcgttat attctaatgt
     6841 gcaagttaca ccatacaatg cttctaatcc ttttgataaa cgtttaattt ctttttcaat
     6901 tgttgctttt gtagcatctg ttaatccacg tacatcacct tcaatttcaa caacatcttt
     6961 aataacattg aattgacctt taccgtcaaa tgaaccgatt gtgacaacac cggtttcaaa
     7021 tggacttagt cgtctagata caactgtttg taacgctgtg acgaagtagc tacctgcaac
     7081 aatggcatca ttggccatat gtggtgatga accatgacca cctttacctt gaactttcaa
     7141 tttgaagaat gcgcgtcctg tttgaacata accaggtctg taatacacat tacctgtttc
     7201 cattgtgctc atgacgtgta cacctaatac atgatcaaca ccgtctaata caccattttc
     7261 aatcattgct ttagcaccac ctggtggtac ttcttcagct ggttgatgta tcacaacgac
     7321 ttttcctgta aaactatctt tcatttcggc aagcgtctct gctaatacaa gcatgtatgc
     7381 tgtatgtgca tcgtgaccac atgcgtgcat aacaccttta ttttgtgatg caaaagataa
     7441 tcctgtatct tcagtaatgg gtaatgcatc aaagtctgca cggattgcta atgttttacc
     7501 aggtttccct gaatcaatcg ttactttaat tccacgtggt ccgacattcg tttctacttc
     7561 tacatcttta cctttgtaaa attcagcgat gtatttcgcc gtttcatcct catgaaaaga
     7621 taattctgga tgctgatgta aataacgtct gatctcaatc attttgtctt cttttgattt
     7681 taaagcttca attaattgtt gattcatatc attcatctcc ttagttacat catatatgat
     7741 tgatcattat ttatattgcc aacaacagag atgttaacca ttaatttttt gcaattttaa
     7801 ctttgaatac aaaaaatcac aaattatgta taacaaaatt tgcgatttgt gatcatttta
     7861 tgaacttggg taacgtttta cttcaattaa gtgaatccca ttcgtaatca ttttaatgtt
     7921 taatgccagc gtatccgtca catctatatc gtacacttct aattttggaa aactcattcg
     7981 attaacgtaa tctatagagt ccttgtccat gccatgtatc gtatgatgtt ttcgccaaag
     8041 attaaataat gcaccatttt ctttatctaa ggtaaaatgt ttaatcttat atataccttc
     8101 ttctagggca tttatgttca aatgaatcat ttccgtcgca cgcatattca tttgattgtc
     8161 caacgctaag tatggattaa aatgctttgc atcatataac aatatttgaa aatttgaatc
     8221 agttcccgtg acaatacatg tatcatcaga atataaaata ttgcttgtta atttattaaa
     8281 taacaatgcc gtgaaataga ccggacgttt tccattatat tgatgaaaca gttcaataga
     8341 attcatataa tcccgttcat ttttacaatg actgacgtgc aaatcataat tcaaccaata
     8401 cccgatcccc tctacctttg agcttaattt taataattgc tcaatgatga taccacctct
     8461 aaaatattcg ccgtttgtaa taaatgtatc acccgtcaat gtattccaat tgagtaaaat
     8521 gagtggacgc tttaggtgat gacgatgcat taagtcgata aggtaattcg ttttattaat
     8581 aatcatttga ctcgcggttt taaattcatc atcattcatt ttattaaaat caacagcatc
     8641 atttgaatta gcatcaaata caaaatggtc gatgtgtggc tcaagtcgtt tcaacaatgg
     8701 tagatgtctt tccgtagctt gatctaagtg aacgtacaag ccaccattgg ggaataatgc
     8761 tttaaaataa tcaatcattt caatcaaaga attgtgcaat gtcgtcacat acaagttgaa
     8821 cttcaaatct tttctatgac tgacatgcag ggcaacgtga tggataaata ctttgaatgc
     8881 atcgatataa tctcgtgagt catactgatt caaatgcatg gttagactaa aattatgatc
     8941 taataaaaag tctaaacaca aatcaatatc ataaaatata ttcgaaattt ctgcatcata
     9001 cgtgaatggc gcattgagct ttttcataat atatggcatc acatcatatg ctaatacttc
     9061 attgacttga aaatcactat gacatgtaag caactgtgat tgatactgtg tattgagcaa
     9121 attcctcaaa tagcccactt gaataatatg attaaattga tttagttggt gattggttgg
     9181 ttgaaaggta atctctttat agttcacctt ttcaatatct tcaatgaaat gattcatttc
     9241 ttcaatgtag tcatttaaaa gtaatatcaa ttcacggtcg tgataattat gttgtgccga
     9301 ttgctgattt tcagtgattg ctggacgatc acctcgatat tgtttaggcg tttgatgcgt
     9361 aaagtgttta aatgttctcg caaagctcgc tgcacttgaa aagccatttt gcaatgcaat
     9421 atcagtaatc gtttcatctg tgtatgtcaa atcgagtttc gcatgctcaa ttcgcgtcgt
     9481 atttaagaaa tgctggaatc ctacacctag cgattctgca aactttttag acagatggct
     9541 ctctgaccac ccaacatatt cgcttaattc cgaaaggctt aagtcttcat gaaaatgtaa
     9601 ctcgatatag tcgcatactt gattcacttt atcatcattt aagatacttt ggttcgaatg
     9661 atatgtacgc ggaacataat gaatcatatg cataagcaac tgaatgacaa gttgttgctc
     9721 agtcaattta gacaactcat tatgtcgaat atgtgttgaa accagtcttg ccattatatt
     9781 tctcagttga tgtatatttt ttgttgtggt cgcatctgtt aagtgaaaat atagacaatg
     9841 cacatcatca aacttgtctg ctaaatattt catttggaat tggatataac atatgatgcc
     9901 atcttgttga agttgaaatc gatacaagtc gcggtggtta atgatgaaaa tgtcgccact
     9961 gttgcattgc gtcatattat tttcatcata aatgtgtgcc tcacctttaa taacaaaacc
    10021 aatcattaaa ccattgagcc ttttgaaatc taacatactc tcagtttcta ctcgaattaa
    10081 ataatcacgt tgcatactat ccctcaattc agtaatatga atacgtttat tttacattat
    10141 tttacagcaa catatttgaa tttcatattg aatcgtgtgt gtggatgatt atttatcctc
    10201 acttggttca agatgtaggc tatcactaaa gtaaatattt tctccttttt tctctacaaa
    10261 cgtaaattca atatctttat atccaactga tgagcctttt aaattacctg tacctttcaa
    10321 caataacttt ggtgctttat ttgttggtat atcatatctt ttacgtaatt gctttacatt
    10381 ataatcatca ttcgttaatt gatatttggc tgaataactc ggcacctctg gattatatga
    10441 aatatctccc tctttgtact tcgacaaatc tttaaagctg ccatattgcg cgaagaactt
    10501 aaagttctcg atttcttttt ttatgttctc atctttgata tcttttgttg gaatgatttt
    10561 attatcgacc attttaacag gatacttttt ttcgttatca cgatgtatac ctttatcttg
    10621 tctttctata acatcaacat agtaatagcc atttgttgtt cttgaatttc tattcatata
    10681 taaaaccata ccttttacgt tcatatcttt tccattaggt tgaatcgaca ttgacgaacg
    10741 aacaatccat gtacctttat catttttatc aaattgatca tcacgataac cttctttatc
    10801 gtataaatcc tctagatttt taatcggata catactcaat gtcttctcaa aacttttctt
    10861 aatttccgct tctttaccca tgccgcaacc agcagtaata ctaatgacta aaaacagaag
    10921 attaatacct agcactaatt tgtttaatcg tttcatcatc ttacaactct cttcttttta
    10981 aaatgattta cttttgcaaa ttacatatta atatcaattt ataacacttt ttcactttaa
    11041 tattatcaaa aattccacaa acaatctgtt
//
LOCUS       DATTQF010000083        11056 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.083, whole genome shotgun sequence.
ACCESSION   DATTQF010000083 DATTQF010000000
VERSION     DATTQF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.083"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            423..2612
                     /locus_tag="VB241_001785"
     CDS             423..2612
                     /locus_tag="VB241_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP1245077.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2624..3076
                     /locus_tag="VB241_001786"
     CDS             2624..3076
                     /locus_tag="VB241_001786"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP1245078.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQDSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVNINNDGPVTIIYESQDGKIQ"
     gene            3073..3948
                     /locus_tag="VB241_001787"
     CDS             3073..3948
                     /locus_tag="VB241_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEP1245079.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVSKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4409..5671
                     /locus_tag="VB241_001788"
     CDS             4409..5671
                     /locus_tag="VB241_001788"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP1245080.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5687..7453
                     /gene="aspS"
                     /locus_tag="VB241_001789"
     CDS             5687..7453
                     /gene="aspS"
                     /locus_tag="VB241_001789"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP1245081.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFEAENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7531..7721
                     /gene="ssrS"
                     /locus_tag="VB241_001790"
     ncRNA           7531..7721
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VB241_001790"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7786..7914
                     /locus_tag="VB241_001791"
     CDS             7786..7914
                     /locus_tag="VB241_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245082.1"
                     /translation="MGLIAFNNCFLVEFWKNNQSNNAKHKILSYYVLMYKSVKEVK"
     gene            7914..8687
                     /locus_tag="VB241_001792"
     CDS             7914..8687
                     /locus_tag="VB241_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEP1245083.1"
                     /translation="MKHQFSRNELAIGHQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8848..10122)
                     /locus_tag="VB241_001793"
     CDS             complement(8848..10122)
                     /locus_tag="VB241_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP1245084.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSERQLKEIYNNLLKQRP"
     gene            10207..10629
                     /locus_tag="VB241_001794"
     CDS             10207..10629
                     /locus_tag="VB241_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEP1245085.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10729..10911
                     /locus_tag="VB241_001795"
     CDS             10729..10911
                     /locus_tag="VB241_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP1245086.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            10951..>11056
                     /locus_tag="VB241_001796"
     CDS             10951..>11056
                     /locus_tag="VB241_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245087.1"
                     /translation="MSFIDKAKDAVEKFKNSDNEQVKNVKDKINEYTGS"
BASE COUNT         3974 a         1534 c         2158 g         3390 t
ORIGIN      
        1 aatataattt tatcaaataa aatccttcat caaatgaata agaacgaatg acttaattaa
       61 aaaagttgtt taagttttct taacatgaga tgttaggatt ttttatttac tgaaaatgtt
      121 agatgattga gcattatacc ttaataacaa cgtttattta tttcataaat tgtagtatca
      181 tagaactaat atttaaaaaa tgaaacagta gatttaggtc gaatttttgt aaaagtttta
      241 aaagtaggaa tagtatacaa attaaactcg ctcaagtaaa attaatatta cgattaatga
      301 cgacaggata aatatttatc gtcgacggac gtatgattgg tgtgggacaa atactattca
      361 acaagagcac ctaaatcatt gtttaaggcg aagtaataaa tatgaatggg gtgtatcata
      421 taatgaacaa cgaatatcca tatagtgcag acgaagttct tcacaaagca aaatcatatt
      481 tgtcagcaga tgaatatgag tatgttttaa aaagctatca tattgcttat gaagcacata
      541 aaggtcagtt ccgaaaaaac ggattaccat acattatgca tcctatacaa gttgcaggta
      601 ttttaacaga aatgcgatta gacggaccga cgattgtcgc aggttttttg catgatgtaa
      661 ttgaagatac accgtataca tttgaagatg taaaagaaat gttcaatgaa gaagttgctc
      721 gaattgttga tggtgtgacg aagcttaaaa aagtaaaata ccgatcaaaa gaagaacaac
      781 aagctgaaaa tcatcgcaag ttatttattg cgattgccaa agatgtacgc gtaattttgg
      841 tgaaattagc agacagatta cataatatgc gtaccttgaa agccatgccg cgcgaaaaac
      901 aaattagaat ttctcgagaa acattagaaa tttatgcacc tttagcacat cgtcttggta
      961 ttaatacaat caaatgggaa ctagaagata cggctcttcg ttatattgat aatgtacaat
     1021 attttagaat agtcaattta atgaagaaga aacgtagtga acgggaagcg tatatcgaaa
     1081 cggctattga tagaatacgt accgaaatgg accgaatgaa tatcgaaggc gatataaatg
     1141 gtagacctaa acatatttac agtatttatc ggaaaatgat gaagcagaaa aagcaatttg
     1201 atcaaatttt tgatttgttg gcgatacgtg ttattgtcaa ttctattaat gattgttatg
     1261 cgatacttgg gttggtgcat acgttatgga aaccgatgcc aggacgtttt aaagattata
     1321 ttgcaatgcc taaacaaaat ttgtatcagt cattgcatac tacagtagta ggtccaaatg
     1381 gagacccgct cgaaatccaa atacgaacgt ttgatatgca cgaaattgct gaacatggtg
     1441 ttgcagcaca ctgggcttac aaagaaggta aaaaagtaag tgaaaaagat caaacttatc
     1501 aaaataagtt aaattggtta aaagagttag ctgaagcgga tcatacttcg tctgacgctc
     1561 aagaatttat ggaaacctta aaatatgact tacagagtga caaagtatat gcatttaccc
     1621 cagcgagtga tgttattgag ttgccatatg gtgctgtgcc gattgatttt gcttatgcga
     1681 ttcacagtga agtaggtaat aagatgattg gtgccaaggt gaatggcaaa attgtaccaa
     1741 ttgactatat tttacaaaca ggcgatattg ttgaaatacg tactagtaaa cattcatatg
     1801 gaccaagtcg tgattggttg aaaattgtta aatcgtctag tgccaaaggt aaaattaaaa
     1861 gtttcttcaa aaaacaagat cgttcatcta acattgaaaa aggacgaatg atggttgaag
     1921 ctgaaataaa agagcaagga tttagagtcg aagatatttt gacagagaaa aatattcagg
     1981 ttgttaatga aaaatataac tttgcaaatg aagatgattt attcgcagct gtaggatttg
     2041 gcggcgtgac atccttacag attgttaata aattaactga aagacaacgt attttagata
     2101 agcaacgtgc tttaaatgaa gcacaagaag ttacgaaatc attgcctatt aaagacaaca
     2161 tcattactga tagtggtgtc tatgtagaag gtttagaaaa tgtacttatc aagttgtcaa
     2221 aatgttgtaa tcctatacca ggtgatgata ttgtaggtta tatcaccaaa ggtcatggta
     2281 ttaaagtaca tcgcactgat tgcccaaata ttaagaacga aactgaacga ctaattaatg
     2341 ttgaatgggt aaaatcaaaa gacgcaactc aaaaatatca ggttgattta gaggtaactg
     2401 cgtatgaccg aaatggcttg ttaaatgagg tactacaagc tgttagctcg acagccggca
     2461 atttaattaa agtttcaggt cgttcagata ttgataaaaa tgcaataata aatattagtg
     2521 tcatggtgaa aaacgtgaat gatgtttatc gtgtggtaga aaagatcaaa caacttggtg
     2581 atgtttatac agtaacaaga gtttggaact agaggtgcaa aatatgaaag tagttgtaca
     2641 aagagttaaa gaagcatcgg tgacgaatga tacattaaat aatcaaatca aaaaaggata
     2701 ttgtttatta gtcggtatcg gtcaggactc tacagagcaa gatgcagatg taattgcaaa
     2761 gaaaattgct aatgcaagat tatttgaaga tgacaataat aaattaaact ttaatatcca
     2821 acaaatgaat ggtgaaatat tatcagtttc acaatttact ctctatgcag atgtaaaaaa
     2881 aggtaaccgt ccaggtttct caaattctaa aaatcctgat caagcagtaa aaatatatga
     2941 gtattttaat gatgcgctac gagcgtatgg tctaactgtg aaaacaggtg aatttggaac
     3001 acacatgaat gttaatataa ataatgatgg tccagtcact attatttatg aaagtcagga
     3061 cggcaaaatt caatgaaaaa aatagaggca tggttatcta aaaagggtct taaaaataaa
     3121 cgtactctaa tagtagtgat tgcctttgtc ttatttatca tctttttatt tttattgctg
     3181 aatagcaata gtgaagatag tgggaacatt acgataactg aaaatgctga attacgtaca
     3241 ggtccaaacg ctgcgtatcc agtcatttac aaagttgaaa aaggtgacca ttttaaaaag
     3301 attggtaaag taagtaaatg gattgaagtt gaagatacat ccagtaatga aaaaggttgg
     3361 atagctggat ggcacacaaa tttagatatt gtcgcggata atacgaagga gaaaaatcct
     3421 ttgcaaggta aaacaatagt gcttgatcct ggtcatggag gtagtgacca gggtgcttca
     3481 agcaatacta aatataaaag tttagaaaaa gattatacgt tgaaaacagc aaaagaattg
     3541 cagcgtactt tagaaaaaga aggcgcaact gttaagatga caagaacaga cgatacatat
     3601 gtttcactag aaaatcgtga tatcaaaggc gatgcctatt tgagtataca taatgatgcc
     3661 ttagaatcat ctaatgcaaa tggaatgaca gtttattggt atcatgataa tcaaagagct
     3721 ttagcagata cgttagacgc tacgattcag aagaaaggtc tactttctaa tcgcggttca
     3781 agacaagaaa attatcaagt gttaagacaa acaaaagttc ctgctgtttt attagaatta
     3841 ggttatatta gtaacccaac tgatgaaacg atgattaaag atcaattaca tagacaaatt
     3901 ttagaacaag caattgttga tggccttaaa atttattttt ctgcgtaggg cttgcaaaaa
     3961 tatgtgaaag tagttatcat tgatattgaa ttttataact aaaaccgtta gtattcttga
     4021 aattgtaaat gaaataggta gcaatctaac taagattgtg taggaatata atccatagac
     4081 tgaaagatta tgctgagtag tttatataca ttgaacacaa gaagaggtgc tttatgaaaa
     4141 gtaaagccgt taaacgtacg ttaaacgttt tgagtgggtt tattaaatga acgcttataa
     4201 aaagtaatga tgattacaat taggcatgtt ttttaaacca atgagggtgg caacacggat
     4261 attcgtccct tgtgcagttt caggctgtgc aagggctttt tcttttgctt ttaaaattat
     4321 gatttatcgt gcaaagttaa gtggtcgtat atagttttag ttttaaaaag gtaattaaat
     4381 aaaatagttt gccgagggag atgtcaaaat gattaaaata cctagaggga cgcaggatat
     4441 tttacctgaa gattcaaaga aatggcgtta cattgaaaat caattagatg aattaatgac
     4501 attttacaat tataaagaaa taagaacacc aatttttgaa agtacagatc tttttgcaag
     4561 aggtgttggt gattcaaccg atgtcgtaca aaaagaaatg tatacattta aagataaagg
     4621 cgatagaagt attacattaa gacctgaggg aacagctgca gttgtgcgtt catatattga
     4681 acataaaatg caaggtaatc caaaccaacc aattaaactt tattacaatg gaccgatgtt
     4741 tagatatgaa cgtaagcaaa aagggcgcta tcgtcaattt aatcaatttg gtgtagaagc
     4801 tattggtgct gaaaatccta gtgtagatgc agaagtatta gctatggtta tgcatattta
     4861 tcaatcattt ggattaaaac atttaaagct tgttattaat agtgtagggg atatggcgtc
     4921 tcgaaaagaa tataacgaag cgttagtgaa acactttgaa ccagtaattc atgaattttg
     4981 ttcagattgt caatcacgtt tgcatacaaa tccgatgcga attttggatt gtaaagttga
     5041 ccgtgataaa gaagcgatta agactgcacc tagaatcact gatttcttaa atgaggaatc
     5101 taaggcatat tatgaacaag taaaagctta tttagatgat ttaggtattc catatattga
     5161 agatcctaac ttagttcgtg gattggatta ttatacacat acagcatttg aattaatgat
     5221 ggataaccct aactatgatg gtgccattac aactctttgt ggtggtggcc gttataatgg
     5281 tttattagaa ttgctagatg gtccaagtga aacaggtatt ggttttgcgc taagtataga
     5341 gcgattattg cttgcacttg aagaagaagg tatcgaatta gatattgaag aaaacttaga
     5401 tttattcatt gttacaatgg gtgatcaagc agatcgatat gctgtgaagt tattaaatca
     5461 tttgagacat aatggtatta aagcagataa agactactta cagcgtaaaa ttaaaggaca
     5521 aatgaaacaa gcagaccgtt taggtgccaa gtttacaatc gttattggtg atcaagaatt
     5581 agaaaataat aaaatcgatg ttaaaaatat gacaactggt gaatctgaaa caattgaatt
     5641 agacgcatta gtcgaatatt ttaagaagta gagagggcgt taaaatatga gtaagagaac
     5701 aacttattgt ggattagtta ctgaagcatt tttaggacaa gaaattacat taaaaggatg
     5761 ggttaacaat cgtcgtgacc taggtggatt gattttcgtt gatttaagag atagagaagg
     5821 aattgtacaa gttgtgttta atcctgcatt ttcagaagag gcattgaaaa ttgctgaaac
     5881 agtacgttct gaatatgttg tagaagttca aggtacagtt acgaagcgtg accctgaaac
     5941 tgttaatcct aaaattaaaa ctggccaagt tgaagtacaa gttacaaata ttaaagtgat
     6001 taataaatct gagacaccac cattttctat aaatgaagaa aatgttaatg ttgatgaaaa
     6061 tattcgatta aaataccgtt atttagattt acgtcgtcaa gagttagcgc aaacatttaa
     6121 aatgagacat caaattacac gttctattcg tcaatatttg gatgatgaag ggttctttga
     6181 catcgaaaca ccagtactaa cgaagtcaac acctgagggt gcacgtgact atttagtacc
     6241 atctcgtgtc catgatggtg aattttatgc attaccacaa tcgccacaat tatttaagca
     6301 attattgatg attagcggat ttgacaaata ctaccaaatt gtaaaatgct tccgtgacga
     6361 agatttacgt gcagatcgtc aacctgaatt cacacaagtc gatattgaaa tgagttttgt
     6421 agatcaagaa gatgtgatgc aaatgggtga agaaatgctt aaaaaagttg ttaaagaagt
     6481 taaaggcgtt gaaattaatg gcgctttccc acgcatgaca tataaagaag cgatgcgtcg
     6541 ctatggttct gataaaccag atacgcgttt tgaaatggaa ttaattgacg tttctcaatt
     6601 aggacgtgat atggacttta aagtatttaa agatactgtt gaaaatgatg gtgaaattaa
     6661 agcaattgtc gctaaaggtg cagctgaaca atatacacgt aaagatatgg atgctttaac
     6721 agaatttgta aacatctatg gtgctaaagg attagcatgg gttaaagttg tggaagatgg
     6781 tttgacaggt ccaattggac gtttctttga ggcagaaaat gttgaaacat tacttacatt
     6841 aactggtgct gaagctggtg acttagtaat gtttgttgca gacaaaccaa atgtcgttgc
     6901 acaaagttta ggtgcattac gtgtcaaatt agctaaagaa ttaggtttaa tcgatgaaac
     6961 aaaattaaac ttcttatggg tgacagattg gccattatta gaatatgatg aagatgcgaa
     7021 acgttacgtt gcagcacatc atccatttac atctccaaaa gaagccgata ttgctaagct
     7081 tggcactgcg ccagaagaag ctgaggcaaa tgcttatgac atagtattaa atggttatga
     7141 attaggtggc ggttcaatca gaattcatga cggtgagtta caagaaaaaa tgttcgaagt
     7201 tcttggattt actaaagaac aagcacagga acaattcggc ttcttactag atgcatttaa
     7261 atatggtgca ccacctcatg gcggtattgc attaggatta gaccgattag tcatgttatt
     7321 gactaataga acaaacttga gagatacaat tgcattccct aaaacagcat ctgctacatg
     7381 tttattaacg aatgcgcctg gtgaagtttc tgataaacaa ttagaagaac tttctttgcg
     7441 aattcgtcac taaattttaa aaaatcaata aactttggtt tgttataatt tcattaattt
     7501 taattatgtg gtaatataac atcataagat agtcatctga gttgttcgta agtttgcttt
     7561 ttaatttggg cctaacactc tttgatcaag ggagcccaat gggttttctt gcagcgtaca
     7621 cgccttatat gaaggacgta caaaacaaga aacagggcac ccacctgtat ataacaggcc
     7681 gaatgatcaa gctattataa ctacggcaat acggactcta tcgatacgca agactttgtc
     7741 ttgcgtattt ttatgctttt ctttaagttt ttaataaaga attagttggg tttaatagcg
     7801 tttaataatt gtttcctcgt tgaattttgg aaaaataatc aaagcaataa tgctaaacat
     7861 aaaattttaa gttactatgt acttatgtat aaatcagtga aagaggtaaa ataatgaaac
     7921 atcaattttc aagaaatgaa ctagcgattg gacatcaagg tttagattta ttgaagcaac
     7981 aaactgttgt catacttggc gttggaggtg ttggttcatt tgctgcggaa gcattagcaa
     8041 gaacgaatat tggtcatatt atattaatag ataaagatga tgttgatatt acaaatgtaa
     8101 acagacaatt acacgcttta actacaacgg taggacaaag taaagtttct ttgatggaag
     8161 aacgtattaa attaattaat ccagattgta aagtcacgcc tttacatatg ttttatacag
     8221 aagagacata tgaagaaatt ttcgataatt acgatataga ttattttgta gatgcaagtg
     8281 atacaataat gtacaaagta cacttaatga aagagtgtct tgaccgaggg attaaagtga
     8341 tttccagtat gggtgctgca aataaaacag atccaacacg ttttacaatt tcagatatct
     8401 ctaaaacaca taccgatcca atggccaaaa ttattcgtca aaaattgaaa aaactaggaa
     8461 ttcgtaaagg gattccagtt gtattttcag atgaaagtcc aattgtcata agagaagatg
     8521 taaaagatat agttggagat aaaaatgcta tcaatcgaaa aggacaaatg ccaccttctt
     8581 caaatgcctt tgtgccaagt gtagttggat taatttgtgc aagttatgtg gtgaatgacg
     8641 tattaaaaga tattccagtt cgtcgcatta aagacaaagg gcaataatta aaattgaaag
     8701 tcataatttt caacgtaagg caagtgtaac cataccataa aactaaaact gactagttcg
     8761 caagcattgt agaatatgcc tcgtgtacta gtcagttttg ttttgattat agctaaaata
     8821 tcaaaggtaa tcgatttaat cttattttta tggcctttgt ttaagtaaat tgttatatat
     8881 ttctttgagt tgtcgttcac ttttagatgt cgtttttggt tcataataaa ttttgttttt
     8941 aagtttatca ggtaaatatt gctgtgaaac atagccatta acatattggt gtggatattt
     9001 gtaaccaata gatcggccta aatctttagc accttgataa tgtccatctt ttaaatggtt
     9061 tggaatttgg cccacatgac cgtttctaat atcggacaat gcactatcaa tggcactcat
     9121 tgctgaattt gacttaggtg ataagcatag ttcgattact gcttggctta gtgggattct
     9181 agcttctggt aaacctagac gttctgctga ttcaatagca gcaagtgttc tctgaccagc
     9241 attaggtgag gctaagccta tatcctcata gctaattaca agtaatcgtc gaactattgt
     9301 aggtaaatct ccagcttcaa ttaatcgtgc taaataatgt aaagcggcat tgacgtcgct
     9361 accacggata gatttttgga aagcgctcat aacatcgtaa tgcatgtcac catccttatc
     9421 acttacaaat gcaccttttt gtaagcagtc tttagcatct tgcaatgtaa catgtcgata
     9481 accgtccttg tcattatctg cgcttaatac agctaattcc aatgcattta acgcactacg
     9541 aacatcacct tgactttgtg tagaaaagta ggtcatggca tcttcatcaa ttttgggttg
     9601 atatgttttc aaaccattct cttcatcttc tattgcacga gttaacgctt ggcgcacatc
     9661 ttcgtcattt aaaggatata actcgaaaat ttgcgctctt gaacgaatcg ctggattgat
     9721 agcatgataa ggatttgaag ttgtagcacc gatcaagacg attttgccat tttctaaatg
     9781 aggtaataaa aaatcttgtt tggctttatc tagtcgatgg atttcatcta ataacaagat
     9841 aacttgacca gacattttag cttcttcaac aacaagttgc atatcttttt tagtgttagt
     9901 tacagcattc aattgtctga atttatattg cgtactgccc gaaattgctt tggcaatact
     9961 tgttttgcct ataccaggtg gaccataaaa aatcattgaa gttaattttt ttgtgtcaac
    10021 cattcttctg ataatgcctc ttggtccaac taagtgttgt tgggaaatga tttcatctat
    10081 attttttggg cgcattctcg acgctaatgg ttctgtactc acatttgtca cacctttctt
    10141 atactatctt aactcaaaaa atgctaaaat gggaatatca atattcgaat taagtgaggt
    10201 tatacaatga aaatttctac taaagggaga tatggactta cattgatgat ttctcttgct
    10261 aaaaaagagg ggcaaggatg tatatcatta aagtcaattg ctgaagaaaa taatttgagt
    10321 gatttatatt tagaacagct tgtaggtcct ttaagaaatg cggggttaat tcgaagtgta
    10381 cgcggtgcta aaggtggata ccaattaaga gtgccagcgg aagaaatctc agcaggggat
    10441 attataagac tgttagaagg tccaattaca tttgttgaaa gtattgaatc agaaccacct
    10501 gcgcaaaaac aactatggat tcgcatgaga gatgcagtga gagatgtttt agataataca
    10561 acattgaaat atttagcgga atatgtagat acaagtgaag atttagacgg atacatgttt
    10621 tatatttaac cgtataaata ctatactata acataaaaac ttcatattat aatgtttagc
    10681 gaacctcctt tgtggtatat aaatatatac atctaaggag gtttcactat ggcagacgaa
    10741 agtaagttcg atcaatttaa aggcaatgtt aaagaaacag taggtaacgt tactgataac
    10801 aaagaattag aaaaagaagg tcagcaagat aaagcgactg gtaaagcaaa agaagttgtt
    10861 gaaaatgcta aaaacaaaat aactgatgca attgataaac ttaaaaaata agttcaatta
    10921 aactaatcaa atataagagg agcgattaat atgagtttta tagataaagc taaagacgca
    10981 gtagaaaaat ttaaaaatag cgataatgaa caagttaaaa atgtaaaaga taagataaat
    11041 gaatatacag gatcta
//
LOCUS       DATTQF010000084        10728 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.084, whole genome shotgun sequence.
ACCESSION   DATTQF010000084 DATTQF010000000
VERSION     DATTQF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.084"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            111..203
                     /locus_tag="VB241_001797"
     CDS             111..203
                     /locus_tag="VB241_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245088.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(285..785)
                     /gene="ftnA"
                     /locus_tag="VB241_001798"
     CDS             complement(285..785)
                     /gene="ftnA"
                     /locus_tag="VB241_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HEP1245089.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEEAMFETHINYLTRIGDDSNALYLYEKELGT
                     RTFDEE"
     gene            1077..2390
                     /gene="murT"
                     /locus_tag="VB241_001799"
     CDS             1077..2390
                     /gene="murT"
                     /locus_tag="VB241_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HEP1245090.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNGDDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISRFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            2392..3123
                     /locus_tag="VB241_001800"
     CDS             2392..3123
                     /locus_tag="VB241_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP1245091.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEKQAKQVLIDRANRQKKSR"
     gene            3209..3319
                     /locus_tag="VB241_001801"
     CDS             3209..3319
                     /locus_tag="VB241_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245092.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            3348..3761
                     /locus_tag="VB241_001802"
     CDS             3348..3761
                     /locus_tag="VB241_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245093.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEIKFEFALFDSDMDNLKYIEYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMVTESGVSLRINVTKSGFTKFINELVSLH"
     gene            complement(3950..4936)
                     /locus_tag="VB241_001803"
     CDS             complement(3950..4936)
                     /locus_tag="VB241_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP1245094.1"
                     /translation="MNGQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQEIILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            5636..6394
                     /gene="map"
                     /locus_tag="VB241_001804"
     CDS             5636..6394
                     /gene="map"
                     /locus_tag="VB241_001804"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEP1245095.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            6611..6997
                     /locus_tag="VB241_001805"
     CDS             6611..6997
                     /locus_tag="VB241_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245096.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMHYLITK
                     KEQLSKLIDSLMLTLENKYIDIVEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            7012..7713
                     /locus_tag="VB241_001806"
     CDS             7012..7713
                     /locus_tag="VB241_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP1245097.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            7710..8753
                     /locus_tag="VB241_001807"
     CDS             7710..8753
                     /locus_tag="VB241_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1245098.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            8743..9372
                     /gene="vraR"
                     /locus_tag="VB241_001808"
     CDS             8743..9372
                     /gene="vraR"
                     /locus_tag="VB241_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HEP1245099.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(9485..10702)
                     /locus_tag="VB241_001809"
     CDS             complement(9485..10702)
                     /locus_tag="VB241_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP1245100.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDTNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
BASE COUNT         3856 a         1544 c         1908 g         3420 t
ORIGIN      
        1 tcatttggat gtaactctga ttaaaaagta aatgatgata tgctttaaaa tcggtcaaat
       61 taatacaaca ttaaatttag agttattaac attagtagtt taaaaaacaa atgactaaaa
      121 atacaataat tagccttgaa aatgaaaaaa cgcagatcaa tgattcagaa aatgaatcta
      181 gtgatctccg taaagcaaaa taatatagac attgggttat tgtaagtgac ccaaatgtct
      241 atcatgtcgt atgatattta tctgtctatt gtagtgatgt ttaattattc ttcgtcgaat
      301 gtacgagtgc caagttcttt ttcgtaaaga tataatgcat tgctgtcatc gccgatacga
      361 gttaaataat tgatgtgagt ttcaaacatt gcttcttctt cgacttgttc atctaagaac
      421 cagtttaaga atgagatagt tgcataatct ttatcttgac gagcgatttc agataagtta
      481 tagaaacgtc ttgttacttc ttgttcttga gataagctgt ctttgaaagt ttctagtatg
      541 cttgaaaagt caatttttgg tgctgaaact gctctgaatt ctgcatgtgc acctctgtcg
      601 ttaatatagt tataaatctt ttgtccatgg aaacgttctt ctttagcttg ttgaatgaag
      661 aagtttgcaa atccttcata cgattcttta tcacagtatg ctgccattgc catatatgcg
      721 tgcgctgcaa agtactcatg gttcatttga tcatttaatg cttctaataa atttttactt
      781 aacattttga tacacctctt atttgtaaat taaaattact atgaattaaa cttagtataa
      841 caaactcatt ctcaattgta aataataata attataattt aatgactgat tttagcactc
      901 taattgagaa aatgtatcat ttaccgagat ttaattgaga ataaatatca tttttaatta
      961 tctaaatgag aatgattata atttagaaac actgtatcaa tttgtgattg agaaactttt
     1021 ttgaagttgc cttgtcatgt tataatgatg cgagtggaaa tttgaggagg aaacacatga
     1081 gacagtggac ggcaatccat ctagcgaaat tggcgcgtaa agcaagtaga gcagtaggta
     1141 aaagaggaac agatttacct ggacaaatcg ctagaaaagt ggatacagat atattaagaa
     1201 aattagcaga gcaagttgat gatattgtat ttatcagtgg aacaaatggt aaaacaacga
     1261 cttcaaactt aattggacat actttaaaag cgaataatat tcaaattata cacaataatg
     1321 aaggtgctaa tatggctgca ggtataactt ctgcattcat catgcaatca acacctaaga
     1381 ctaaaattgc ggtaatcgaa attgatgaag gttcgattcc acgtgtgtta aaagaagtta
     1441 caccttcaat gatggtattt actaatttct ttagagatca aatggatcgt ttcggtgaaa
     1501 ttgatattat ggttaataac attgcagaga caattagtaa taaaggcatc aaattattgc
     1561 taaatggtga tgatccattt gtgagtcgtt tgaaaatcgc aagtgatacg attgtgtact
     1621 atggtatgaa agcacatgct catgaatttg aacaaagtac gatgaatgaa agtagatatt
     1681 gtccaaactg tggtcgctta ttgcaatatg attatattca ttataatcaa attggtcatt
     1741 atcactgtca gtgtggtttc aaacgagagc aagcaaaata tgaaatatca agatttgatg
     1801 tggcaccgtt tttacattta aatatcaatg atgaaaaata tgatatgaaa atagcaggtg
     1861 actttaacgc ttataacgcg ttagcagcat atacagtttt aagagagcta gggttaaatg
     1921 aacaagcaat taaaaatggc tttgaaacgt atacatcaga caatggtcgt atgcagtact
     1981 ttaaaaaaga acgaaaagaa gcgatgatca atttagctaa aaaccctgca ggaatgaatg
     2041 caagtttatc agttggtgaa caattagaag gcgaaaaagt gtatgttatt tcgctaaatg
     2101 ataacgctgc agatggtcga gatacttcat ggatttatga tgcagatttc gaaaaattat
     2161 ctaagcaaca aattgaagct atcatcgtga caggtacacg agcagaagaa cttcaattgc
     2221 gattgaagtt agcagaggtt gaagtaccaa ttatagttga gcgtgatatt tataaagcaa
     2281 cggcaaagac tatggattat aaaggtttca cagttgcaat accaaactat acatcattag
     2341 cgcctatgct tgaacaatta aaccgttcgt ttgaaggagg tcaatcataa tatgcatgaa
     2401 ttgactattt atcattttat gtcagataaa ttgaatttat acagtgatat aggaaatatt
     2461 attgctttaa gacaacgtgc aaaaaaacga aatattaaag ttaatgtcgt agaaatcaat
     2521 gaaacagaag gtattacctt tgatgaatgt gatattttct ttatcggtgg tggaagtgat
     2581 agagaacaag cattagcaac aaaagaacta agtaaaatta agacaccact taaagaagcg
     2641 attgaagatg gtatgccggg attaacgatt tgtggaggct atcaattttt aggaaaaaaa
     2701 tatatcacgc ctgatggtac agaattagaa gggttaggta ttttagattt ttatactgaa
     2761 tcaaagacaa accgattaac aggagatatt gttatcgaaa gtgatacttt tggaactatt
     2821 gtaggttttg aaaatcacgg tggtagaaca tatcataatt tcggtacact tggtcatgtt
     2881 acttttggtt atggtaataa tgatgaagat aaaaaagaag gcattcatta taaaaattta
     2941 ttaggtactt atttacacgg accaatttta cctaaaaatt acgaaataac tgattatctg
     3001 ttagaaaaag cttgtgaacg taagggtatt ccgtttgagc ctaaagaaat agataatgaa
     3061 gcggaaaagc aagcgaaaca agtattaata gacagagcaa atagacagaa gaagtctcgt
     3121 taactctgaa catcgcatca atgggtttaa aattgataaa cgatgaagtt tagtaattaa
     3181 tcatatatgt ataaacacac acattatttt ggatggaaac aaccaaattg atgtgtgttt
     3241 ttttgttcta gtgaataatt attatacaat gagtatctat cctagaatta tcaatagtaa
     3301 tggtgattat gcaacatgaa aaaatgaatg atgaaaggaa tttgacgatg aagcctacta
     3361 aagtgatatt aaaagatgca tcttatttac atagcaaaac atcgataaca tttattttaa
     3421 aagatgtagt tatcgaagaa gataataaaa tttattattt cgacactagt gcaactttcg
     3481 aagatcaaga aattaaattt gaatttgcac tttttgatag tgacatggac aatttaaaat
     3541 atattgaata cgacaatcca gttaccgaaa tatattttat agaacctgat ttacatttta
     3601 cgattattga ttttaatcaa gaactgcttt gtatttatat tgattttgat tctggtttaa
     3661 ggcattcaaa catggtaaca gaatctggtg tttcgttaag gataaacgtt actaaatcag
     3721 gttttactaa atttattaat gaattagtct cgttacatta atgatttaaa tctgatacac
     3781 aattacaatc aaaaaagaca gccacatccc tccgtagttt aggcgtgtgg ctatatttga
     3841 gtctgaatat atatgcttgt aattttaaaa agggacatgc tatatacgat aaaattactg
     3901 ataaagtaag aatatatcta atgattccaa ttagcataag gcacttaaat tatgcatatc
     3961 tgctgtctag cagtcgataa atcattaaaa cttcatacag tatatgactt ttaatttgat
     4021 tttcaccact aatttcaagt gcttttattg tcgaacgtaa agtttctaca gaatcatctt
     4081 ctctcttaaa tgaaccatca taaaaaatat ctttgatgct actactaatt tttagcaatg
     4141 ccattttttc gtcacctgaa aagttaacac gagtattttt aggcaagtaa atgatatttg
     4201 ataaatgagt gataaacaaa cgattcgtat atgcacgttt agttaattga ttgagcaatt
     4261 tccaatcaca ttcttttttc ttatgatagc ttaattcatc acgttgataa cttattaacg
     4321 tttcaacttg attgtttaaa ttgaaaatat ttttatatgc tttttcgctt ttatcagatt
     4381 gcagtcttga taagataatt tcttggcagc gattgtaaaa taatttatac atcaaggcat
     4441 ctgtcttact taatttttct tcgacctgac cataatactt aggtggaaac accatgaagt
     4501 taattaaacc tgatgtcacg agtccaataa tcgctgtcaa tgttcgagac aaaaagttga
     4561 atatgtaggc atcatgaata cctggaatca tagctaatga tgttagtaca gcaacattcg
     4621 taccaacttg caatttgagt tttgtacaga ataaaatcgt gaacgtcgca ctcaatgcat
     4681 atgtaaaagg tgattgatcg ccgaataaat atgtaaataa tactgcaaag cctgcaccaa
     4741 ttaccgtagc aggtaatcta cgataacctt ttataagtga tgccttggca gttggttcaa
     4801 ttgtgactac agctgttaaa atggcataga tgggtgttaa atctagtgcc atacaaaaga
     4861 cagccgttaa aaaaatggca ataccagttt taattgttct ggcaccaatt aaatgtttat
     4921 accattgacc gttcattttt taacctctaa tcatcgtaaa atcttagcga gcgctttata
     4981 ataatagtat cgtacattgg aaaagttcat gtatgtaaaa catttgaaat aatcatgctt
     5041 atgcattact ttgactttca tatacattaa tcaaagctct gggctccaca atgttatatt
     5101 gttagtagtt gactgaaata aaatgcactt gtaacaagca tttttcaatt ctagtccggg
     5161 gccccaacat agagaaattg gaacaccaat ttctgcagac aatgcaagtt ggggaggggc
     5221 cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg cggggcccca
     5281 acatagagaa attggatcta caatttctac aggcaatgca agttggggtg tgggccccaa
     5341 cacagagaat ttcgaaaaaa gaaattctac aggcaatgca ggttggggtg taggccccaa
     5401 cacagagaaa ttggaacacc aatttctata ggcaatgcaa gttggcgggg ccccaacaca
     5461 gagaatttca aaaaagaaat tctacaggca atgcaagttg gggacgatta agaaataatt
     5521 ttctttatca cactatgtcc cactcacttt ccaaaatact aaagtaacat ctttagtata
     5581 tcaaagaatt tttgctataa taagttataa ttatataaaa aaggaacggg ataaaatgat
     5641 tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt ggatacatat gcgctaaagt
     5701 gcgcaataca atgcaagctg caaccaaacc aggtatcact acgaaagagc ttgataatat
     5761 tgcgaaagag ttatttgaag aatacggcgc tatttcagcg ccaattcatg atgaaaattt
     5821 tcctggtcaa acgtgtatta gtgttaatga agaggtggca catgggattc caagtaagcg
     5881 tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg gcattgaaga atggctatta
     5941 tgcagataca ggcatttcat ttgtcgttgg agaatcagac gatccaatga aacaaaaagt
     6001 atgtgacgta gcaacgatgg catttgagaa tgcaattgca aaagtaaaac cgggtactaa
     6061 gttgagtaac attggtaaag cggtgcataa tacagctaga caaaatgatt tgaaagtcat
     6121 taaaaactta acaggtcatg gtgttggttt atcattacat gaagcgccag cacatgtact
     6181 taattacttt gatccaaaag acaaaacatt attaactgaa ggtatggtat tagctattga
     6241 accgtttatc tcatcaaatg catcattcgt tacagaaggt aaaaatgaat gggcttttga
     6301 aacgagcgat aaaagttttg ttgctcaaat tgagcataca gttatcgtga ctaaggatgg
     6361 tccgatttta acgacaaaga ttgaagaaga atagttcaac atatactaag actaaagtat
     6421 gaacatcatt tagttccgga gcctattcat attggtttcg gaactgtttt ataataatta
     6481 agaacacaat caatgcgtca tttcaaaaat atgttgtaat aaagtagttt ataagcaaac
     6541 atatcatcga catcaacgaa gatacatagc gcatttggta ctttaaaact tattataaaa
     6601 ggtgatagtt atgaactatg ttgaacgtta tattgaacag tttttgagag caacagtaag
     6661 aaataatatc aagcactacc ttttaatgtt agatgaaaaa atgaaaaatt tagatgatta
     6721 tatgcattat ttaattacta aaaaagaaca acttagcaag ttaattgaca gtctaatgct
     6781 aacattagaa aataaatata ttgatattgt tgaagcattt caaattcaat gtgcaagaga
     6841 aatcaataat caagaaattg aaaatattaa atcagagttg aataaagttg aagcatatta
     6901 tgcacaaatt gaaactcaaa ttcaacaaac ttcaactgaa aaaatagcaa cagaaaaaac
     6961 atcgtatcta ataaattata tgaacgctgt ggcatagaaa ggcggcgaaa catgacacac
     7021 aaatatatat caacgcaaat gttgatcatt tttactgcat taatgattat tgccaatttt
     7081 tactacatat tttttgaaaa aattggcttt ttactcgttc tattattggg atgtgtatta
     7141 gtttatgtag gatatcttta ttttcataaa atacgtggcc ttttggcgtt ttggataggc
     7201 gcgctattaa ttgcattcac attattgtct aataagtata caatcatcat cttgttcgtc
     7261 tttttattac tacttattgt gcgttattta atacacaagt ttaaacccaa aaaagtagtt
     7321 gcgacggatg aggttatgac ttcaccatct tttattaaac aaaagtggtt tggtgagcaa
     7381 cgtacaccag tttatgtata taagtgggaa gatgtccaaa ttcaacatgg aattggcgac
     7441 ctacatattg acttaacaaa agctgcaaat attaaggaaa ataataccat tgttgttaga
     7501 cacattttag gtaaagtgca ggttatattg ccggttaatt acaatattaa tttacatgta
     7561 gctgcttttt atggaagtac ttacgtgaat gaaaaatcat ataaagttga aaataacaat
     7621 attcatattg aagaaatgat gaaaccggat aactatacag ttaatatcta cgtatcaacg
     7681 tttatcggag acgtagaggt gatttatcga tgaaccacta cattagaaca attggttcaa
     7741 tgctcatctt agtatatagc atgctagctg catttctgtt catcgataaa gtttttgtaa
     7801 atatcatcta ttttcaaggt atgttttata cacaaatatt cggaatacca gtctttttat
     7861 ttttaaatct catcatcata ttattgtgta ttattgttgg ttcggtactc gcttacaaaa
     7921 tcaatcagca aaatgattgg attaagacgc aaattgagcg ttcaatggaa ggcgaaacag
     7981 ttggcattaa tgatcaaaat atagaaatat atagtgaaac gttagactta taccatacac
     8041 ttgtaccttt aaatcaagaa ttgcataagt tgcgacttaa aactcaaaac ttaaccaatg
     8101 aaaattataa tattaatgat gtgaaagtta aaaagattat tgaagatgaa cgtcaaagac
     8161 tagcacgaga acttcacgat tctgttagtc agcaactttt tgcggcaagt atgatgcttt
     8221 ctgctatcaa agaaacgaag ttagaaccac cattagacca acaaattccg attttagaga
     8281 aaatggttca agattcgcag ctagaaatgc gtgctttgct gttacattta agaccgcttg
     8341 gtttaaaaga caaatcttta ggtgagggta ttaaagattt agttattgat ttacaaaaaa
     8401 aagtgccaat gaaagttgtg catgaaatac aagattttaa agtgcctaaa ggtattgaag
     8461 atcatttgtt cagaattaca caggaagcaa tttcgaatac attgcgtcat tcaaacggta
     8521 caaaagtgac agtagaattg tttaataaag acgattattt attgttgaga attcaagaca
     8581 atggtaaagg ttttaatgtt gatgaaaaat tagaacaaag ttatggactt aaaaatatgc
     8641 gtgaaagagc tttggaaatt ggtgcaacgt tccatattgt atcattgcca gattcaggta
     8701 cacgtatcga ggtgaaagca cctttaaata aggaggattc gtatgacgat taaagtattg
     8761 tttgtggatg atcatgaaat ggtacgtata ggaatttcaa gttatctatc aacgcaaagt
     8821 gatattgaag tagttggtga aggcgcttct ggtaaagaag caattgccaa agcccatgag
     8881 ttgaagccag atttaatatt aatggattta cttatggaag acatggatgg tgtagaagcg
     8941 acgactcaga ttaaaaaaga tttaccgcaa attaaagtat taatgttaac tagttttatt
     9001 gaagataaag aggtatatcg tgcattagat gcaggtgtcg atagttacat tttaaaaaca
     9061 acaagtgcaa aagatatcgc cgatgcagtt cgtaaaactt ctagaggaga atctgttttt
     9121 gaaccggaag ttttagtgaa aatgcgtaac cgtatgaaaa agcgcgcaga gttatatgaa
     9181 atgcttacag aacgagaaat ggaaatatta ttattgattg cgaaaggtta ctcaaatcaa
     9241 gaaattgcta gtgcatcgca tattactatt aaaacggtta agacacatgt gagtaacatt
     9301 ttaagtaagt tagaagtgca agatagaaca caagctgtta tctatgcatt ccaacataat
     9361 ttaattcaat agttcttatc gaattaagaa aagttactta cgccaatcac aatataacat
     9421 caaatataca ctaaaaaaca gaggtcttcg caatcattag cgaaaacctc tgttttgttg
     9481 ttatttagtc ttttttaagc ttatcaacga ttttgtcttt tatattttta tcatcttctg
     9541 gataggtatc actaggatgt tcgtcaacat gatacgttcg ttgatgtgtt gcattagtat
     9601 cttcatcata ttcgtaccta gtgttctcat tataatgatt tttattattg tcatcatgtt
     9661 ctaatgctgc ttcttcaggt gttttacctt taattacact acgctgatga atgattgcat
     9721 tgatttcagc accgacaatt ataataaaac ttgtgatata taaccatagt aacaaaatga
     9781 tgatacctgc gatactgcca tatgttttag aatagttacc aaaatttgaa atataccaac
     9841 caaaaccaaa tgacccagcc aaccaaataa ttgaagtaaa tactgcacct ggtaatactg
     9901 acttaagttt cgttttaacg ttaggtgcaa ccgaatataa cacgataaat acgataaata
     9961 taataatgat tggtaacaca attctaataa gattaaaaat ccatttcact tgttcgtcaa
    10021 atccaagtgg accgaatagg aaatgactaa ttacagaacc aagcgttggt aatgctagag
    10081 caactacaaa cacaacgccc ataactacag tgaagacaac acttagtagt tttaatacga
    10141 ttccattacg gctatcttct acatcgtaag caacattgaa agaattcata attgcagtca
    10201 ttccatttga agctgaccaa attgctaaaa tcaaaccgat agataataag ccaccactgg
    10261 agttttgagt tatatcacca attacactct taattagagt agatgtttca gcgggtgcat
    10321 tacttaacat attagtaatt tgactctgct taatattgaa aaatggtaat aatgttaata
    10381 ggaaaagcaa catagggaac atagcaagta cgaaatggta agtcatttgc gctgctagtc
    10441 ctgaggcatc atcttttcct atgcgataga tgagatatga taagaaatta gagtctttct
    10501 tatattttgc aggtttattt aatcttgata agaagaaaac ctgatcatct tttttaggtt
    10561 tcttagattg gaattgttga ggttcaatat atgtacgatc aacatcaatt ttgggattac
    10621 ttttattttt cttttttgac tcctcttgcg cttccttaac agaattaagg tattttgaag
    10681 aagagtggtc ctttttcgac atgtgcactc cttaaccttt ctctatgt
//
LOCUS       DATTQF010000085        10708 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.085, whole genome shotgun sequence.
ACCESSION   DATTQF010000085 DATTQF010000000
VERSION     DATTQF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.085"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..168)
                     /locus_tag="VB241_001810"
     CDS             complement(<1..168)
                     /locus_tag="VB241_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEP1245101.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVY"
     gene            255..1331
                     /locus_tag="VB241_001811"
     CDS             255..1331
                     /locus_tag="VB241_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEP1245102.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPAFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            1396..1707
                     /locus_tag="VB241_001812"
     CDS             1396..1707
                     /locus_tag="VB241_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1245103.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            1807..2664
                     /locus_tag="VB241_001813"
     CDS             1807..2664
                     /locus_tag="VB241_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HEP1245104.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            2693..3331
                     /locus_tag="VB241_001814"
     CDS             2693..3331
                     /locus_tag="VB241_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEP1245105.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEITNEGNVVGYNIFEISKDITIEETGHIKLTDELVNVFQKRISEAGFDYKLNAD
                     LSPKFVVGYVETKDKHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVM
                     PSGMVIKDAELRGVASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            3352..7176
                     /locus_tag="VB241_001815"
     CDS             3352..7176
                     /locus_tag="VB241_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP1245106.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNI
                     YSNNTNHRAKDSTTDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKSSEKVESDKQ
                     KYDKYVAKTQTSQNKHLEQEKQKDSVVKQGTASKSSDENVSSTTKSTPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTEASQLHTNEIEDEAVSNDENKKASIQNEDTDDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSDVTSQRQSALQHNVEVNNQDELKNQSRLIADS
                     EEDGATNEEEYSGSQIDDAEFYELNDTEVDEDTTSNSEDNTNRDASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDGHVVEDSSMNEIEK
                     QNAEITENVQNEAAESKQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAASESKTATQNTPSSSTDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAATENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHQNNTSGQQDNDDQQKDLQLSFSNQNEDTANENRPRTNQPDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            7200..8513
                     /locus_tag="VB241_001816"
     CDS             7200..8513
                     /locus_tag="VB241_001816"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP1245107.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKESDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            8587..9078
                     /locus_tag="VB241_001817"
     CDS             8587..9078
                     /locus_tag="VB241_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP1245108.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            9152..10342
                     /locus_tag="VB241_001818"
     CDS             9152..10342
                     /locus_tag="VB241_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245109.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQTETDKSAAVSNEESKASALKAQQAAI
                     KEEASANNLSDTSQEAQAIQEAKKEAQAETDKSADVSNEESKASTLNVSKEESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPLLVTKKNDVSETVNTDNKD
                     TVKQKEAKFENGVITRKADEKTPNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQ
                     KKQHNKKASQGAKKQSSSSNSTTKTNQKNSKATNAKSSNASKKSNAKVEKAKSKIEKR
                     TFND"
     gene            10498..10581
                     /locus_tag="VB241_001819"
     CDS             10498..10581
                     /locus_tag="VB241_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245110.1"
                     /translation="MLFETSLDNNSDIAHFKLMMEKYLIIK"
BASE COUNT         4201 a         1473 c         1983 g         3051 t
ORIGIN      
        1 ataaacgata tatgagcctt tgacattcat aaaatatgat tttacttcat taagtacttt
       61 aacgggatta tagcgtttac gattaataaa ttggatgatt gaaatggtag aaataattac
      121 aatggctact gcaattattg ttggaattat atatttcagt ttagtcatca tttatgctcc
      181 cttgatttca aattcatatc attagtttac catattgaag atgatataat aacttataag
      241 gagtgagaac ttttatgaac ataaataaaa aagtaacatt acaacgaatt caaactttaa
      301 ctgagcttca tggggcacca ggttttgaag aagaagtaaa aaattatatg actcagcaaa
      361 tggcgccgta cgtagatgaa tttattgaaa atcgtatggg tggttttttt ggtgttaaaa
      421 aatctaaaaa tccaaatgca aaacgtgtaa tgattgcagc acatatggat gaaattggat
      481 tcatgattac aaacatcact aaaaatggaa tgattcaatt cacaaattta ggtggtgttg
      541 caaatgatat ttggcaagga caacgcttag taattaaaaa tagaaatggc gataaaatta
      601 tcggtgttgt ttctaatata cctaaacatt ttcgtactgg tagtgaaggt gcaccggaaa
      661 ttaaagattt aacattagat ataggtgctc aaaatgaaga tgaagtgcgt gagcgcggaa
      721 tagatatagg agatacaatt gtacctcaca cgccattcac acagttatct gaacatcgat
      781 atagtgctaa agcatgggat aatcgttatg gttgtgtctt ggcaattgaa atactagaat
      841 tattaaaaga tatagaatta gatgtagact tgtatgttgg cgcaaatgtt caagaagagg
      901 ttggattacg aggtgcgaaa gcatctgcag agatgataga cccagacgtt gcatttgttg
      961 tcgattgttc acctgccaat gacgttaaag gtaatcaacc attatccggt gaacttggta
     1021 aaggtacgtt aattcgcata aaagacggta caatgatttt aaagcctgca tttagagact
     1081 atttattaaa gttagtagaa gcacatgaca ttgaacatca atactatatg tcacctggtg
     1141 gaacagatgg tggagaaatt cataaagcta atattggtat tccaactgca gttattggtg
     1201 tatgtgcacg atatattcat agtacagact cagtatttga tataagagac tattttgctg
     1261 ctagatcttt actttcagaa gccatttgta atttagataa caatcaaata gaaacattac
     1321 aatataaata atcgggtaat aacaactatt atttctaaat agttatatat aatcattaat
     1381 taaggagaca taaaaatgaa acaacttgaa tcagaacaac aatttgaatc tttaaaacaa
     1441 ggtgctacag tatttgaatt cactgcaggc tggtgtccag attgtagagt gatagaacca
     1501 gatttaccgg aattagaagc gagatatcct atgtttgact tcgtatcagt agaccgtgat
     1561 aaatttatgg atatttgtat tgaaaatggt attatgggga ttccaagttt tctagtatat
     1621 aaaaatggag aactgcttgg aagttatatt ggaaaagaac gaaaatcaat tgaacagata
     1681 gatgcatttt tagctcaata cgtgtaattt agactagaga aaaacggggt aatactcgtt
     1741 tttctctgtt actatgtgtt gatttattgt aaactattat aaggtgcgaa attaggagtg
     1801 ttacatatga atacctttca aatgagagat aaattaaagg aacgtttaag ccatttagac
     1861 gttgatttta aatttaatcg tgaagaagaa actttgcgta tttatcgaac agataataac
     1921 aaaggtatca cgattaaact taacgctata gtcgcaaaat atgaagataa aaaagaaaaa
     1981 attgtagatg aaattgttta ttacgttgat gaagctattg cacaaatggc agataaaacc
     2041 cttgagagta tatcatctag tcaaattatg cctgtcatta gagcgactag ctttgataaa
     2101 aaaactaaac aaggtgttcc ttttatctat gatgaacata ctgcagaaac agcagtttat
     2161 tatgcagtcg atttaggaaa atcatatcgt ctaattgatg aaagcatgtt agaggatttg
     2221 aagttaactg aacaacaaat aagagaaatg tctctgttta atgttagaaa attatcaaat
     2281 tcatatacga ctgatgaagt aaaaggtaat attttttact ttattaactc aaatgacggg
     2341 tatgatgcaa gtaggattct aaatactgca tttttaaatg aaattgaggc acaatgtcaa
     2401 ggtgaaatgc tcgtagcagt gccacaccaa gatgtgttaa ttattgcaga tatacgcaat
     2461 aaaacaggat atgatgtgat ggcacattta acaatggaat ttttcactaa aggtctagtt
     2521 ccaattacat cattatcctt tggatataaa cagggtcatc ttgaaccgat atttatttta
     2581 ggtaaaaata ataaacaaaa aagagatcca aacgtgattc agcgtttaga agcaaatcgt
     2641 cgtaaattta ataaagataa atagaaataa ttggataagg agttttgtca taatgaattt
     2701 attttacaat cctaaatatg taggagatgt cgcattttta caaattgaac cagttgaagg
     2761 tgaattaaac tacaataaaa aaggtaacgt tgttgaaatt actaatgaag gtaatgttgt
     2821 aggttataat atttttgaaa ttactaatga aggtaatgtt gtaggttata atatttttga
     2881 aatttcaaaa gatataacaa ttgaagaaac aggtcatatt aaattaactg atgaacttgt
     2941 aaatgtattc caaaagcgta tttcagaagc tggttttgat tataaattaa atgctgatct
     3001 atcaccgaaa tttgtagttg gctacgttga aactaaagac aaacatcctg atgcagataa
     3061 attaagtgta ctaaatgtaa atgttggaaa tgacacatta caaattgtat gtggcgcgcc
     3121 taacgttgaa gctggacaga aagttgttgt tgctaaagta ggtgcagtga tgcctagcgg
     3181 tatggtaatt aaagatgctg aattacgtgg tgttgcctca agcggtatga tttgttcaat
     3241 gaaagaattg aatttaccta atgcacctga agaaaaaggt attatggtat taaatgacag
     3301 ctatgaaatt ggacaagcat tttttgaata attaaggaag gtagtgaaaa tatgagctgg
     3361 tttgataaat tattcggcga agataatgat tcaaatgatg acttgattca tagaaagaaa
     3421 aaaagacgtc aagaatcaca aaatatagat aacgatcatg actcattact gcctcaaaat
     3481 aatgatattt atagtcgtcc gaggggaaaa ttccgttttc ctatgagcgt agcttatgaa
     3541 aatgaaaatg ttgaacaatc tgcagatact atttcagatg aaaaagaaca ataccatcga
     3601 gactatcgca aacaaagcca cgattctcgt tcacaaaaac gacatcgccg tagaagaaat
     3661 caaacaactg aagaacaaaa ttatagtgaa caacgtggga attctaaaat atcacagcaa
     3721 agtataaaat ataaagatca ttcacattac catacgaata agccaggtac atatgtttct
     3781 gcaattaatg gtattgagaa ggaaacgcac aagtcaaaaa cacacaatat atattctaat
     3841 aatacaaatc atcgtgctaa agattcaact acagattatc acaaagaaag tttcaagact
     3901 tcagaggtac cgtcagctat ttttggcaca atgaaaccta aaaagttaga aaatggtcgt
     3961 atccctgtaa gtaaatcttc agaaaaagtt gagtcagata aacaaaaata tgataaatat
     4021 gtagctaaga cgcaaacgtc tcaaaataaa catttagaac aagagaaaca aaaagatagt
     4081 gttgtcaagc aaggaactgc atctaaatca tctgatgaaa atgtatcatc aacaacaaaa
     4141 tcaacaccta attattcaaa agttgataat actatcaaaa ttgaaaacat ttatgcttca
     4201 caaattgttg aagaaattag acgtgaacga gaacgtaaag tgcttcaaaa gcgtcgattt
     4261 aaaaaagcgt tgcaacaaaa gcgtgaagaa cataaaaacg aagagcaaga tgcaatacaa
     4321 cgtgcaattg atgaaatgta tgctaaacaa gcggaacgct atgttggtga tagttcatta
     4381 aatgatgata gtgacttaac agataatagt acagaggcta gtcagcttca tacaaatgaa
     4441 atagaggatg aagctgtatc aaatgatgaa aataaaaaag cgtcaataca aaatgaagac
     4501 actgatgaca ctcatgtaga tgaaagtcca tacaattatg aggaagttag tttgaatcaa
     4561 gtatcgacaa caaaacaatt gtcagatgat gaagttacgg tttcggatgt aacgtctcaa
     4621 cgtcaatcag cactgcaaca taacgttgaa gtaaataatc aagatgaact aaaaaatcaa
     4681 tccagattaa ttgctgattc agaagaagat ggagcaacga atgaagaaga atattcagga
     4741 agtcaaatcg atgatgcaga attttatgaa ttaaatgata cagaagtaga tgaggatact
     4801 acttcaaata gcgaagataa taccaataga gacgcgtctg aaatgcatgt agacgctcct
     4861 aaaacgcaag agcacgcagt aactgaatct caagttaata atatcgataa aacggttgat
     4921 aatgaaattg aattagcgcc acgtcataaa aaagatgacc aaacaaactt aagtgtcaac
     4981 tcattgaaaa cgaatgatgt gaatgatggt catgttgtgg aagattcaag catgaatgaa
     5041 atagaaaagc aaaacgcaga aattacagaa aatgtgcaaa acgaagcagc tgaaagtaaa
     5101 caaaatgtcg aagagaaaac tattgaaaac gtaaatccaa agaaacagac tgaaaaggtt
     5161 tcaactttaa gtaaaagacc atttaatgtt gtcatgacgc catctgataa aaagcgtatg
     5221 atggatcgta aaaagcattc aaaagtcaat gtgcctgaat taaagcctgt acaaagtaaa
     5281 caagctgcga gtgaaagcaa gactgcgact caaaacacac catcatcaag tactgattca
     5341 caagagtcaa acacgaatgc atataaaaca aataatatga catcaaacaa tgttgagaac
     5401 aatcaactta ttggtcatgc agcaacagaa aatgattatc aaaatgcaca acaatattca
     5461 gagcagaaac cttctgctga ttcaactcaa acggaaatat ttgaagaaag ccaagatgat
     5521 aatcaattgg aaaatgagca agttgatcaa tcaacttcgt cttcagtttc agaagtaagc
     5581 gacataactg aagaaagcga agaaacaaca catcaaaaca atactagtgg acaacaagat
     5641 aatgatgatc aacaaaaaga tttacagctt tcattttcaa atcaaaatga agatacagct
     5701 aatgaaaata gacctcggac gaatcaacca gatgttgcaa caaatcaagc tgtacaaact
     5761 tctaagccga tgattcgtaa aggcccaaat attaaattgc caagtgtttc attactagaa
     5821 gaaccacaag ttattgagcc ggacgaggac tggattacag ataaaaagaa agaacttaat
     5881 gacgcattat tttactttaa tgtacctgca gaagtacaag atgtaactga aggtccaagt
     5941 gttacaagat ttgaattatc agttgaaaaa ggtgttaaag tttcaagaat tacggcatta
     6001 caagatgaca ttaaaatggc attggcagcg aaagatattc gtatagaagc gccaattcca
     6061 ggaactagtc gtgttggtat tgaagttccg aaccaaaatc caacgacggt caacttacgt
     6121 tctattattg aatctccaag ttttaaaaat gctgaatcta aattaacagt tgcgatgggg
     6181 tatagaatta ataatgaacc attacttatg gatattgcta aaacgccaca cgcactaatt
     6241 gcaggtgcaa ctggatcagg gaaatcagtt tgtatcaata gtattttgat gtctttacta
     6301 tataaaaatc atcctgagga attaagatta ttacttattg atccaaaaat ggttgaatta
     6361 gctccttata atggtttgcc acatttagtt gcaccggtaa ttacagatgt caaagcagct
     6421 acacagagtt taaaatgggc cgtagaagaa atggaaagac gttataagtt atttgcacat
     6481 taccatgtac gtaatataac agcatttaac aaaaaagcac catatgatga aagaatgcca
     6541 aaaattgtca tagtaattga tgagttggct gatttaatga tgatggctcc gcaagaagtt
     6601 gagcagtcta ttgctagaat tgctcaaaaa gcgagagcat gtggtattca tatgttggta
     6661 gctacgcaaa gaccatctgt caatgtaatt acaggtttaa ttaaagccaa cataccaaca
     6721 agaattgcat ttatggtatc atcaagtgta gattcaagaa cgatattaga cagtggtgga
     6781 gcagaacgct tgttaggata tggcgatatg ttatatcttg gtagcggtat gaataaaccg
     6841 attagagttc aaggtacatt tgtttctgat gacgaaattg atgatgttgt tgattttatc
     6901 aaacaacaaa gagaaccgga ctacctattt gaagaaaaag aattgttgaa aaaaacacaa
     6961 acacaatcac aagatgaatt atttgatgat gtttgtgcat ttatggttaa tgaaggacat
     7021 atttcaacat cattaatcca aagacatttc caaattggct ataatagagc agcaagaatt
     7081 atcgatcaat tagagcaact cggttatgtt tcgagtgcta atggttcaaa accaagggat
     7141 gtttatgtta cggaagcaga tttaaataaa gaataattat gagtaaggag ttttatataa
     7201 tgacacacta tcattttgtc ggaattaaag gttctggcat gagttcatta gcacaaatca
     7261 tgcatgattt aggacatgaa gttcaaggat cggatattga gaactacgta tttacagaag
     7321 ttgctcttag aaataagggg ataaaaatat taccatttga tgctaataac ataaaagaag
     7381 atatggtagt tatacaaggt aatgcattcg cgagtagcca tgaagaaata gtacgtgcac
     7441 atcaattgaa attagatgtt gtaagttata atgatttttt aggacagatt attgatcaat
     7501 atacttcagt agctgtaact ggtgcacatg gtaaaacttc tacaacaggt ttattatcac
     7561 atgttatgaa tggtgataaa aagacttcat ttttaattgg tgatggcaca ggtatgggat
     7621 tgcctgaaag tgattatttc gcttttgagg catgtgaata tagacgtcac tttttaagtt
     7681 ataaacctga ttacgcaatt atgacaaata ttgatttcga tcatcctgat tattttaaag
     7741 atattaatga tgtttttgat gcattccaag aaatggcaca taatgttaaa aaaggtatta
     7801 ttgcttgggg tgatgatgaa catctacgta aaattgaagc agatgttcca atttattact
     7861 atggatttaa agagtcggat gacatttatg ctcaaaatat tcaaattacg gataaaggta
     7921 ctgcttttga tgtgtatgtg gatggtgagt tttatgatca cttcctatct ccacaatatg
     7981 gtgaccatac agttttaaat gcattagctg taattgcgat tagttattta gagaagctag
     8041 atgttacaaa tattaaagaa gcattagaaa cgtttggtgg tgttaaacgt cgtttcaatg
     8101 aaactacaat tgcaaatcaa gttattgtag atgattatgc acaccatcca agagagatta
     8161 gtgctacaat tgaaacagca cgaaagaaat atccacataa agaagttgtt gcagtatttc
     8221 aaccacacac tttctctaga acacaagcat ttttaaatga atttgcagaa agtttaagta
     8281 aagcagatcg tgtattctta tgtgaaattt ttggatcaat tagagaaaat actggcgcat
     8341 taacgataca agatttaatt gataaaattg aaggtgcatc gttaattaat gaagattcta
     8401 ttaatgtatt agaacaattt gataatgctg ttattttatt tatgggtgca ggtgatattc
     8461 aaaaattaca aaatgcatat ttagataaat taggcatgaa aaatgcgttt taatatgttt
     8521 ataatagagt agtatgggta tttattatta atgacattat tacatgttaa ttaggaggcg
     8581 tttttaatgg attggatttt accaattgct ggaattatcg ctgcgattgc attcttaatt
     8641 ttatgtatcg gtatcgtagc tgtattaaat tctgttaaga aaaacttaga ttatgttgca
     8701 aaaacacttg acggtgtaga aggtcaagtt caaggtatta ctcgtgaaac aacagattta
     8761 cttcataaag taaaccgttt aactgaggat atccaaggta aagtagatcg tttaaactca
     8821 gtagtagatg ctgttaaagg tatcggtgac tcagtacaaa cgttaaacag ctctgtagat
     8881 cgtgtaacaa attcaattac acataatatt tctcaaaatg aagataaaat ctcacaagtt
     8941 gttcaatggt caaatgttgc aatggaaatt gcagacaaat ggcaaaatag acactaccgt
     9001 cgtggaagtg caaattacaa agctaataat gtagcaactg atgcaaatca tagctatact
     9061 tctcgagtag ataaataatt aataattaaa caaatgcact taaatgaggt tgttacttat
     9121 ttaagtgcat ttgttatagg aggatataca aatgacgcaa caacaaaata ataaaagaac
     9181 attaaaaaat aaacacactt atcaaaatga accattacca aaccgtaaag attttgttgt
     9241 tagttttata actggcgcgc ttgttggttc agctttaggc ttatatttta aaaataaagt
     9301 ttatcaaaaa gcagatgatt taaaagtcaa agaacaagaa ctgtcgcaaa agtttgaaga
     9361 aagaaaaacg caacttgaag aaacggttgc ctttacaaaa gaacgtgttg aaggattttt
     9421 aaacaaatct aaaaatgaac aagcggcatt gaaggcacaa caagcagcaa taaaagaaga
     9481 agcaagtgca aataatttaa gcgatacatc acaagaggca caagagattc aagaagctaa
     9541 aagagaagca caaacagaaa cggataaaag tgcggctgta tcaaatgaag agtcaaaggc
     9601 atcggcattg aaggcacaac aagcagcgat aaaagaagaa gcaagtgcaa ataatttaag
     9661 tgatacatca caagaagcac aagcgattca agaagcgaag aaagaagcgc aagcagaaac
     9721 agataaaagt gcagatgtat caaatgaaga atcaaaagca tcgacattaa acgtatcgaa
     9781 agaagagtca caagctgaaa gattagcaaa cgctgcaaaa cagaagcaag ctaaattaac
     9841 accaggctca aaagagagtc aattaactga agcgttattt gcagaaaaac cagttgctaa
     9901 aaatgacttg aaagaaattc ctctattagt tactaaaaag aatgatgtat cagaaacagt
     9961 taatacagat aataaagaca ctgttaaaca aaaagaagct aaatttgaaa atggtgttat
    10021 tacacgtaaa gctgatgaaa aaacacctaa taatacagct gttgacaaga aatcaggtaa
    10081 acaatctaaa aaaacaacac cttcaaataa acgaaatgca tcaaaagcat cgacaaataa
    10141 aacttcaggt cagaaaaagc aacataataa gaaagcatca caaggtgcaa agaaacaaag
    10201 tagttcaagt aactcaacga caaagactaa tcaaaaaaat tcaaaagcaa caaatgctaa
    10261 atcatccaat gcatcaaaaa aatcaaatgc taaagttgaa aaagctaaaa gtaaaataga
    10321 gaaacgtaca tttaatgact aagcatacta aaatcgttta ataatcaatc atagcagtca
    10381 tctatgtatt atatttttaa aaattgaatt tcaaaataaa aggtaggcat tcaggtgctt
    10441 accttttttg atatcatcag tttattttga aaggcaacgc gatcatttca tgtatttatg
    10501 ttgtttgaaa catctttaga taacaatagt gatattgcac attttaagct gatgatggaa
    10561 aaatatttga taatcaaata aataatgaat ctaagtaggt acaatgacgt tcactaattc
    10621 aaatatggct gaatatgttg gtgagtgatg ttcactatag atttatatta atatacaaag
    10681 gttgttaatt tttattaagc gttaggtt
//
LOCUS       DATTQF010000086        10597 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.086, whole genome shotgun sequence.
ACCESSION   DATTQF010000086 DATTQF010000000
VERSION     DATTQF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.086"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            131..2740
                     /gene="adhE"
                     /locus_tag="VB241_001820"
                     /gene_synonym="adhC"
     CDS             131..2740
                     /gene="adhE"
                     /locus_tag="VB241_001820"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP1245111.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEIN
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTSIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGLAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            3085..3753
                     /gene="capA"
                     /locus_tag="VB241_001821"
     CDS             3085..3753
                     /gene="capA"
                     /locus_tag="VB241_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HEP1245112.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGALFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            3769..4455
                     /gene="cap8B"
                     /locus_tag="VB241_001822"
     CDS             3769..4455
                     /gene="cap8B"
                     /locus_tag="VB241_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HEP1245113.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKSLIIDGDMRKPTQNYIFNEQNNNGLSNLII
                     GRTTMSEAITTTEIDNLDLLTAGPVPPNPTELIGSERFKELVNLFNERYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKTLMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            4458..5222
                     /gene="cap8C"
                     /locus_tag="VB241_001823"
     CDS             4458..5222
                     /gene="cap8C"
                     /locus_tag="VB241_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HEP1245114.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLRQATTQGVTEVIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            5242..7065
                     /gene="cap8D"
                     /locus_tag="VB241_001824"
     CDS             5242..7065
                     /gene="cap8D"
                     /locus_tag="VB241_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HEP1245115.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVFVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITFTGIRPGEKMYEELMNKDEIHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            7055..8083
                     /gene="cap8E"
                     /locus_tag="VB241_001825"
     CDS             7055..8083
                     /gene="cap8E"
                     /locus_tag="VB241_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HEP1245116.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQNAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            8096..9205
                     /gene="cap8F"
                     /locus_tag="VB241_001826"
     CDS             8096..9205
                     /gene="cap8F"
                     /locus_tag="VB241_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HEP1245117.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNNRNVELTLNYVDDIVAEIKRAIEGNPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDNEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            9209..10333
                     /gene="cap8G"
                     /locus_tag="VB241_001827"
     CDS             9209..10333
                     /gene="cap8G"
                     /locus_tag="VB241_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HEP1245118.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKIPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIIGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            10336..>10597
                     /locus_tag="VB241_001828"
     CDS             10336..>10597
                     /locus_tag="VB241_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEP1245119.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEN
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENHY"
BASE COUNT         3917 a         1522 c         2034 g         3124 t
ORIGIN      
        1 ttaaaatttg atatttgttc aaataatatt cgaaattaaa cttttttgta tagaattttc
       61 tttatatcct gagagacatg tactataatg tttgtgaaat aattcacaaa gtataaagga
      121 gtggttgtat atgttaacta tacctgaaaa agaaaatcgt ggatcgaaag aacaagaagt
      181 ggcaattatg attgatgctc tagctgacaa agggaaaaag gcattagaag cattatctaa
      241 aaagtcacaa gaagaaatta atcatattgt tcatcaaatg agcttagcag ctgttgatca
      301 acatatggtg ctagcaaaat tagcacatga agaaactgga agaggtatat acgaagataa
      361 agcgattaaa aatttatacg cttctgaata tatatggaat tcaataaaag acaataagac
      421 agtagggatt attggtgaag ataaagaaaa aggattaacg tatgtagcgg aaccaattgg
      481 tgttatttgt ggtgttacgc caacaacaaa tcctacgtcg acaactattt ttaaagcgat
      541 gattgcaatt aagacaggaa atccaatcat ttttgcattc catccaagtg cgcaagaatc
      601 gtcgaagcgt gcagcagaag ttgtattaga agcggcaatg aaggcaggtg cacctaaaga
      661 tattattcaa tggattgaag tgccctctat cgaagcaaca aaacaattaa tgaatcacaa
      721 aggtattgca ttagttctag caacaggtgg ttcgggcatg gttaagtctg catattcaac
      781 tggcaaaccg gcattaggtg tgggaccagg taacgtgccg tcttacattg aaaaaacagc
      841 acacattaag cgtgcagtaa atgatatcat tggttcaaaa acatttgata atggtatgat
      901 ttgtgcttct gaacaagttg tagtcattga taaagaaatt tataaagatg ttactaatga
      961 atttaaagca catcaagcat actttgttaa aaaagatgaa ttacaacgct tagaaaatgc
     1021 aattatgaat gaacaaaaaa caagtattaa acctgatatt gtcggtaaat ctgcagttga
     1081 aatagctgaa ttagcaggta tacctgtccc cgaaaataca aaacttatca tagccgaaat
     1141 tagcggtgta ggttcagact atccgttatc tcgtgaaaaa ttatctccag tattagcctt
     1201 agtaaaagcc caatctacaa aacaagcatt tcaaatttgt gaagacacac tacattttgg
     1261 tggattagga cacacagccg ttatccatac agaagatgaa acattacaaa aagattttgg
     1321 actaagaatg aaagcttgtc gtgtacttgt aaatacacca tcagcggttg gaggtattgg
     1381 tgatatgtat aacgaattga ttccgtcttt aacattaggt tgtggttcgt acggtagaaa
     1441 ctcaatttca cataatgtta gtgcgacaga tttattaaac attaaaacaa ttgctaaacg
     1501 acgtaataat actcaaattt tcaaggtgcc tgctcaaatt tattttgaag aaaatgcaat
     1561 catgagtcta acaacaatgg acaagattga aaaagtgatg attgtctgtg accctggtat
     1621 ggtcgaattc ggttatacaa aaacagttga gaatgtatta agacaaagaa cggaacagcc
     1681 tcaaattaaa atatttagcg aagtcgaacc gaacccatca acgaatacag tatataaagg
     1741 tctggaaatg atggttgatt tccaaccgga tacaatcatt gcacttggtg gtggtttagc
     1801 gatggatgct gcaaaagcaa tgtggatgtt ctttgaacac cctgagacat cattcttcgg
     1861 tgctaaacaa aagttcctag acatcggtaa acgtacttat aaaataggca tgcctgaaaa
     1921 tgcgacgttc atttgtatcc ctacgacatc aggtacaggt tcagaagtaa caccatttgc
     1981 agttatcaca gatagtgaaa caaatgtaaa atatccgttg gctgattttg ctttaacacc
     2041 tgatgttgca attattgacc ctcaatttgt gatgagtgtg ccaaaaagcg ttacagcaga
     2101 tacaggaatg gatgtactaa cgcatgcaat ggaatcatat gtatctgtaa tggcttcaga
     2161 ttacacaaga ggtttgagtc tacaagcgat taaattgacg ttcgaatatt taaaatcatc
     2221 tgttgaaaaa ggtgataaag tttcaagaga gaaaatgcat aacgcatcaa ctttggctgg
     2281 tatggcattt gcaaatgcat tcttaggcat tgcacactca attgcgcata aaattggtgg
     2341 ggaatatggt attccgcatg gtagagcgaa tgcgatatta ctaccgcata ttatccgtta
     2401 taatgccaaa gacccgcaaa aacatgcatt attccctaaa tatgagttct tcagagcaga
     2461 tacagattat gcagatattg ccaaattctt aggattaaaa ggtaatacga cagaagcact
     2521 cgtagaatca ttagctaaag ctgtctacga attaggtcaa tcagtcggaa ttgaaatgaa
     2581 tttgaaatca caaggtgtgt ctgaagaaga attaaatgag tcaattgata gaatggcaga
     2641 gctcgcattt gaagatcagt gtacaactgc taatcctaaa gaagcgctaa tcagtgaaat
     2701 caaagatatc attcaaacat catatgatta taagcaataa tctatctgat aataatcatc
     2761 taactcacct gaaattacaa aagtaaaaaa tgccacataa acttaaagtc gataatcatt
     2821 atacggttat cggcttttct ttattgccaa atcttcagag agatacaacc tagacaatca
     2881 atttttttaa taaagaaagt attaagattg gtacactttt cacaaactat tactacttta
     2941 gagtataatt atttttaatt tcatataaat aaaaaggcga aaataatgcg gtttaaaagt
     3001 aattaattgt ttaaacgata tgtaatatgt aaatactata tatataatac caattttaat
     3061 gaaaattttt aagggaggta aataatggaa agtacattag aattaacaaa aattaaagaa
     3121 gtattacaaa aaaacttgaa gattttaatt attttaccgc tattattttt aattattagc
     3181 gctattgtta catttttcgt cttatcacct aaatatcaag ctaatactca aattttagtg
     3241 aatcaaacta agggtgacaa tcctcagttt atggcgcaag aggttcaaag taatattcaa
     3301 cttgtaaata cgtataaaga aattgttaaa agtcctagaa ttttagatga ggtgtcaaag
     3361 gacttaaatg ataagtattc accatctaaa ttatcgagta tgttgacaat tacaaaccaa
     3421 gaaaatacac aacttatcaa catccaagtt aaaagtggtc ataaacaaga ttcggaaaaa
     3481 attgcgaata gcttcgctaa agttacaagt aaacaaattc cgaagattat gagtgtggat
     3541 aacgtatcaa ttttatctaa agcagacggt acagcagtta aagtcgcacc aaaaactgta
     3601 gtgaatctaa tcggtgcact ctttttagga ttagttgtcg cgcttatata tatcttcttc
     3661 aaagtaattt ttgataagcg aattaaagat gaagaagatg tagagaaaga attaggattg
     3721 cctgtattgg gttcaattca aaaatttaat taaggatggt tgctacttat gtcaaaaaag
     3781 gaaaatacga caacaacact atttgtgtat gaaaaaccaa aatcaacaat tagtgaaaag
     3841 tttcgaggta tacgttcaaa catcatgttt tcaaaagcaa atggtgaagt aaagcgctta
     3901 ttggttactt ctgaaaagcc tggtgcaggt aaaagtacag tcgtatccaa tgtagcgatt
     3961 acttatgcac aagcaggtta taaatcgtta attatcgatg gggatatgcg taagccaaca
     4021 caaaactata tttttaatga acaaaataat aatggattat cgaatttaat cattggtaga
     4081 acgactatgt cagaagctat tacgacgaca gaaattgata atttagattt actaacagct
     4141 ggtcctgtac ctcctaatcc aacagaatta attggttctg aaagatttaa agagttagtt
     4201 aatcttttca atgagcgtta tgacatcatt attgtcgata caccgccagt taataccgtg
     4261 actgatgcac aactatatgc acgtgctatt aaagatagtc tgttagtcat tgatagtgaa
     4321 aaaaatgata aaaatgaagt taaaaaagct aaaacactta tggaaaaagc aggcagtaat
     4381 attctaggcg tcattttaaa taaggcaaaa gtagataagt cttctagtta ttatcactat
     4441 tatggagatg aataggtatg attgatattc ataaccatat attgccgaat atcgatgacg
     4501 gtccgacaaa tgaaacagag atgctggatc ttttaagaca agcgacaaca caaggtgtta
     4561 cagaagtcat tgtaacatca catcacttac atcctcgata taccacacct atagaaaaag
     4621 tgaaatcatg tttaaaccat attgaaagct tagaggaagt acaagcacta aatctaaagt
     4681 tttattatgg tcaggaaata agaattaccg atcaaatcct taatgatatt gatcgaaaag
     4741 ttattaccgg tattaatgat tcacgctatt tactaataga atttccatca aatgaagttc
     4801 cacactatac tgatcaatta tttttcgaat tacagagtaa aggctttgta ccgattattg
     4861 cacatccaga gcggaataaa gcaataagtc aaaaccttga catactatac gatttaatta
     4921 acaaaggtgc tttaagtcaa gtgacaacgg cgtcattagc gggtatttcc ggtaaaaaaa
     4981 ttagaaaatt agcaattcaa atgattgaaa acaatctgac acatttcatc ggttcagatg
     5041 cgcataacac agaaatcaga ccgttcttaa tgaaagactt atttaatgat aagaaattac
     5101 gtgattatta tgaagatatg aacggattta ttagtaatgc gaagttagtt gttgatgata
     5161 aaaaaattcc taaacgaatg ccacaacaag attataaaca gaaaagatgg tttgggttat
     5221 aaacagcaaa tgaggggttt tatggcacat ttatctgtga aattgcggct tttaatacta
     5281 gcattaatcg attcactgat agtgacattt tcagtattcg taagttatta cattttagaa
     5341 ccgtatttca aaacatattc tgtcaaatta ttaatattgg cagctatatc actattcata
     5401 tcgcatcata tttcagcatt tatttttaat atgtatcatc gagcgtggga atatgccagt
     5461 gtgagtgaat tgattttaat tgttaaagct gtgacgacat ctatcgttat tacgatggtg
     5521 ttcgtgacaa ttgttacagg caatagaccg ttttttaggt tgtatttaat tacatggatg
     5581 atgcacttga ttttaatagg tggctcaagg ttattttggc gtatttatcg gaaatacctt
     5641 ggaggtaagt catttaataa gaagccaact ttagttgttg gtgctggtca agcaggttca
     5701 atgctgatta gacaaatgtt gaaaagtgac gaaatgaaac ttgaaccggt attagcagtc
     5761 gatgatgacg aacataaacg taatatcaca attactgagg gtgtaaaagt ccaaggtaaa
     5821 atcgcggata ttccagaact agtgaggaaa tataagatta aaaaaattat cattgcaatt
     5881 ccaactattg gtcaagagcg tttgaaagaa attaataata tttgccatat ggatggcgta
     5941 gagttattga aaatgccaaa tatagaagac gtcatgtctg gtgagttaga agtgaaccaa
     6001 ctgaaaaaag ttgaagtaga agatttactt ggcagagatc ctgttgaatt agatatggat
     6061 atgatatcaa atgaattgac gaataaaact attttagtta cgggcgctgg aggttcaata
     6121 ggatcagaaa tttgtagaca agtttgtaat ttctatccag aacgtattat tctacttggc
     6181 catggtgaaa acagtattta tttaatcaat cgtgaattgc gaaatcgctt cggaaaaaat
     6241 gttgatatcg ttcctattat agcggatgtg caaaatagag cgcgtatgtt tgaaattatg
     6301 gaaatgtata aaccatacgc agtttatcat gcagcagcac acaagcacgt gccgttaatg
     6361 gaagacaacc ctgaagaagc agtacgtaat aatattttag gtacgaaaaa tactgctgaa
     6421 gctgctaaaa atgcagaggt aaagaaattc gttatgattt ctacggataa agccgttaat
     6481 ccacctaatg tcatgggagc ttcaaagcga attgcagaaa tgattattca aagtttaaat
     6541 gatgaaacgc atcgaacaaa ttttgttgca gtgagatttg gtaatgtact tggatcgaga
     6601 ggatctgtga ttccactttt caaaagtcaa attgaagaag gtgggccagt tactgtgaca
     6661 catcctgaaa tgacacgtta ctttatgaca attcctgaag cttctagact agttttgcag
     6721 gcaggggcat tagcagaagg tggcgaagta tttgtgttag atatgggaga accagtgaaa
     6781 atagtagatt tagcacgaaa tttaattaag ttaagtggta agaaagaaga cgatatacgc
     6841 attactttta cagggattag acctggtgaa aaaatgtatg aagaattgat gaataaggat
     6901 gaaattcacc ctgaacaagt atttgaaaaa atttatcgtg gcaaagtaca acatatgaaa
     6961 tgtaatgaag ttgaagcgat tattcaagac atcgtcaatg actttagtaa agaaaaaatt
     7021 attaactatg ccaatggcaa aaagggagat aattatgttc gatgacaaaa ttttattaat
     7081 tactgggggc acaggatcat tcggtaatgc tgttatgaaa cggtttttag attctaatat
     7141 taaagaaatt cgtatttttt cacgcgatga gaaaaaacaa gatgacattc gaaaaaaata
     7201 taataattca aaattaaagt tctacattgg tgatgtgcgt gatagtcaaa gtgtagaaac
     7261 agcaatgcga gatgttgatt acgtattcca tgcagcagct ttaaaacaag tgccgtcatg
     7321 tgaattcttt ccagttgagg cagtgaagac aaatattatt ggtacagaaa atgtcttaca
     7381 aaatgctatt catcaaaatg ttaaaaaagt catatgttta tctacagata aggcagcgta
     7441 tcctattaat gctatgggta tttcgaaagc aatgatggaa aaagtattcg tagctaaatc
     7501 aagaaatatt cgtagtgaac aaacgcttat ttgcggtaca agatacggta atgtaatggc
     7561 ttcaagaggt tcagtaattc ctttgtttat cgataaaatc aaagctggag aacctttaac
     7621 gattacagat cctgatatga caagattttt aatgagctta gaagatgcgg tagaactagt
     7681 tgttcatgca tttaagcatg cagagacagg cgatattatg gttcaaaaag caccaagttc
     7741 aacggtaggg gatcttgcga ccgcattatt agaattgttt gaagctgata atgcaattga
     7801 aatcattggt acgcgacatg gagagaaaaa agcagaaaca ttgttgacga gagaagaata
     7861 cgcacaatgt gaagatatgg gtgattattt tagagtgccg gcagactcca gagatttaaa
     7921 ttatagtaat tatgttgaaa ccggtaacga aaagattacg caatcttatg aatataactc
     7981 cgataataca catattttaa cggtggaaga gataaaagaa aaacttttaa cactagaata
     8041 tgttagaaac gaattgaatg attataaagc ttcaatgaga taggagagat tgacgttgaa
     8101 tattgtaatt acaggagcaa agggttttgt aggaaaaaac ttgaaagcag atttaacatc
     8161 aacgacagat catcatattt tcgaagtaca tcgacaaact aaagaggaag aattagagtc
     8221 agcgttgttg aaagcagact ttgtcgtgca tttagcggga gttaatcgac ctgaacatga
     8281 caaagaattc agcttaggaa acgtgagtta tttagatcat gtacttgata tattaactag
     8341 aaatacgaaa aagccagcga tattattatc gtcttcaata caagcaacac aagataatcc
     8401 ttatggtgag agtaagttgc aaggtgaaca gctattaaga gagtatgccg aagagtatgg
     8461 caatacggtt tatatttatc gatggccaaa tttattcggt aagtggtgta agccgaatta
     8521 taactctgtg atagcaacat tttgttacaa aattgcacgt aacgaagaga ttcaagttaa
     8581 taatcggaat gttgaactaa cgctaaacta cgtggatgat atcgtcgctg aaataaaacg
     8641 tgctattgaa ggaaatccaa cgattgaaaa tggtgtacct acagtaccaa atgtatttaa
     8701 agtgacattg ggagaaattg tagatttatt atacaagttc aaacagtcac gtctcgatcg
     8761 aacattgccg aaattagata acttgtttga aaaagatttg tatagtacgt atttaagcta
     8821 tctacctagt acagacttta gttatccctt acttatgaat gtggatgata ggggatcttt
     8881 tacagaattt ataaaaacac cggatcgtgg tcaagtttct gtaaatattt ctaaaccagg
     8941 aattactaaa ggtaatcact ggcatcatac taaaaacgaa aaatttctag tcgtatcagg
     9001 taaaggggta attcgtttta gacatgttaa tgataatgaa atcattgaat attacgtttc
     9061 tggcgacaaa ttagaagttg tagacatacc agtaggatac acacataata ttgaaaattt
     9121 aggcgacaca gatatggtaa ctattatgtg ggtgaatgaa atgtttgatc caaatcagcc
     9181 agatacgtat ttcttggagg tatagcgcat ggaaaaacta aaattaatga caatagttgg
     9241 tacaaggcct gaaatcattc gtttatcatc aacgattaaa gcatgtgatc aatattttaa
     9301 tcagatatta gtacacactg gtcaaaatta tgattataca ttgaatcaaa ttttctttga
     9361 tgatttggaa ttaagacaac cggaccacta cttagaggca gttggaagta acctcggaga
     9421 aacgatgggg aatattattg cgaagacata tgacgtttta ttacgcgaac aaccagatgc
     9481 acttttaatt cttggtgata caaatagttg tttagcagca gtatctgcta aacgattaaa
     9541 aattcctgtg ttccatatgg aagcgggtaa tagatgcttt gatcagaatg tacctgaaga
     9601 aatcaatcgt aaaattgttg accatgtcag tgatgtgaat ctaccttata cagaacatag
     9661 cagacggtat ttattagatg aaggcttcaa taaagcgaat atctttgtga caggatcacc
     9721 gatgacagaa gtgatagaag cgcatcgaga taaaattaat cacagtgacg ttttaaataa
     9781 actaggatta gaaccgcaac aatacatttt agtatctgcg catagagaag agaatatcga
     9841 taatgaaaag aattttaaat cattaatgaa tgcgataaat gatattgcca aaaagtataa
     9901 aattcctgtg atttattcaa cgcatccaag aagttggaag aaaattgaag aaagtaaatt
     9961 tgaatttgat ccattagtta aacagttaaa gccatttggt ttctttgatt ataatgcatt
    10021 gcaaaaagat gcatttgttg tgctatcaga tagtggaaca ttgtcagaag agtcgtctat
    10081 tttgaagttc cctggtgtcc ttattcgaac ttccacagaa agaccggaag tactagataa
    10141 aggtacggtt attataggtg gtattaccta taacaatcta atccaatccg ttgaactagc
    10201 aagagagatg caaaacaata acgaaccgat gattgatgct attgattata aagacactaa
    10261 cgtttcgaca aaagtagtta aaattattca aagctataaa gatattatca atcgaaatac
    10321 ttggaggaaa tgacgatgag gatagcgatt cttggcgcta ctaacattaa gcatatgtca
    10381 ttattatcac attatttaaa ccacattgat ttgaatatca atgaggtgga cattatatac
    10441 actgacaaat atgatatcga agaaaatatc caaggcatca ataattacta taaatataaa
    10501 gtagatatta aagaagattg gacatttatc aaaaaagcta ttgcttacta tcgatttagg
    10561 ccatacgcta tgaaaattct caaagaaaat cattatg
//
LOCUS       DATTQF010000087        10375 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.087, whole genome shotgun sequence.
ACCESSION   DATTQF010000087 DATTQF010000000
VERSION     DATTQF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.087"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            181..615
                     /locus_tag="VB241_001829"
     CDS             181..615
                     /locus_tag="VB241_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HEP1245120.1"
                     /translation="MKLKSFITVTLALGMIATTGATVASNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(872..1057)
                     /locus_tag="VB241_001830"
     CDS             complement(872..1057)
                     /locus_tag="VB241_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEP1245121.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            1352..2293
                     /locus_tag="VB241_001831"
     CDS             1352..2293
                     /locus_tag="VB241_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP1245122.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2305..3549
                     /gene="fabF"
                     /locus_tag="VB241_001832"
     CDS             2305..3549
                     /gene="fabF"
                     /locus_tag="VB241_001832"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP1245123.1"
                     /translation="MSQNIRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(3604..3975)
                     /locus_tag="VB241_001833"
     CDS             complement(3604..3975)
                     /locus_tag="VB241_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEP1245124.1"
                     /translation="MLKKWLGMALITPMLTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            4218..5144
                     /locus_tag="VB241_001834"
     CDS             4218..5144
                     /locus_tag="VB241_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245125.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            5144..6214
                     /locus_tag="VB241_001835"
     CDS             5144..6214
                     /locus_tag="VB241_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245126.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPLLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            6230..7312
                     /locus_tag="VB241_001836"
     CDS             6230..7312
                     /locus_tag="VB241_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245127.1"
                     /translation="MTERILELNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKLALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            7302..8243
                     /locus_tag="VB241_001837"
     CDS             7302..8243
                     /locus_tag="VB241_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245128.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFEIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPQHFVFSTEEEAARLRKNLLVTQD"
     gene            8262..9917
                     /locus_tag="VB241_001838"
     CDS             8262..9917
                     /locus_tag="VB241_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1245129.1"
                     /translation="MTRKLKTLILIFVATIALSGCANDDGIYSDKGQVFRKILSADLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKKVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNELSFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            10129..>10375
                     /locus_tag="VB241_001839"
     CDS             10129..>10375
                     /locus_tag="VB241_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1245130.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLT"
BASE COUNT         3737 a         1469 c         1958 g         3211 t
ORIGIN      
        1 ttattgtaat gtgaatttat ttgaaaaaat agaaagtatt aacaattata gcttttacat
       61 taattaaaat ttatttttaa aaacaagtaa acaatttaca tacttataat ttttgaaact
      121 tttagatttg tgttatattg attttgtaag atactttaac tcacaaagga gagagattat
      181 atgaaattaa aatcatttat aactgtaact ttggcactgg gcatgatcgc aacgactggc
      241 gctactgtgg caagtaatga ggtatctgca gcagaaaagg acaaactacc ggcaactcaa
      301 aaagctaaag aaatgcaaaa tgttccatat acaattgcag tagatggcat tatggctttc
      361 aatcaatctt acttaaattt accaaaagat agccaattat catatttaga tttagggaat
      421 aaagttaaag ctttattata tgatgaacgt ggtgtaacac ctgagaagat tcgaaatgca
      481 aaatctgccg tttacacgat tacttggaaa gatggcagta aaaaagaagt ggatttaaag
      541 aaagatagtt atacagcaaa cttgtttgat tcaaattcaa ttaagcaaat tgatattaat
      601 gtaaaaacta aataaacact taaaattatc aaattcaaca tgaagcattt aattttacag
      661 tgatgattat aatataattg ccttgataca aagattactc gtaaatgaca tctttgtatt
      721 aaggcttttt ctaaatttaa aagtgatggg ttagaggtca ttgagtttta aaatattcca
      781 aatacaaaac attaatggcc aaaaataaaa gccgccttta tctgggcagc ttcaataata
      841 agaaagacat atttcatttt atactaaata gttattgtga tgaatctttc ggcggtttaa
      901 ttactgcagc gaaaattgct gtgaaaatcg tgaacaatac tgccatgata attggattca
      961 ctacatttaa gctgtctcca cctactaggc tattaagtac aaagttaacc atttgcatta
     1021 ataataatgc ccaaaagaat gttacgaggt gtttcatgtc attctacctc cactttaatt
     1081 atatatattt tattttaagt gaaagttaga aatttgtata gtaacatctc atatattttg
     1141 accatattat acagtttaaa taaatgattt tatctgaatg gctattctaa attaagcgca
     1201 ttaaaatcaa tttcatactg aaatttgacg ataataaagc attaaaattt tattaactag
     1261 tcaatattcc tacctctgac ttgagtttaa aaagtaatct atgttaaatt aatacctggt
     1321 attaaaaatt ttattaagaa ggtgttcaac tatgaacgtg ggtattaaag gttttggtgc
     1381 atatgcacca gaaaagatta ttgacaatgc ctattttgag caatttttag atacatctga
     1441 tgaatggatt tctaagatga ctggaattaa agaaagacat tgggcagatg acgatcaaga
     1501 tacttcagat ttagcatatg aagcaagtgt aaaagcaatc gctgacgctg gtattcagcc
     1561 cgaagatata gatatgataa ttgttgccac agcaactgga gatatgccat ttccaactgt
     1621 cgcaaatatg ttacaagaac gtttagggac gggcaaagtt gcctctatgg atcaacttgc
     1681 agcatgttct ggatttatgt attcaatgat tacagctaaa caatatgttc aatctggaga
     1741 ttatcataat attttagttg tcggtgcaga taaattatct aaaataacag atttaactga
     1801 ccgttctact gcagttctat ttggagatgg tgcaggtgcg gttatcatcg gtgaagtttc
     1861 agaaggcaga ggtattataa gttatgaaat gggttctgat ggcacaggtg gtaaacattt
     1921 atatttagat aaagatactg gtaaactgaa aatgaatggt cgagaagtat ttaaatttgc
     1981 tgttagaatt atgggtgatg catcaacacg tgtagttgaa aaagcgaatt taacatcaga
     2041 tgatatagat ttatttattc ctcatcaagc taatattaga attatggaat cagctagaga
     2101 gcgcttaggt atttcaaaag acaaaatgag tgtttctgta aataaatatg gaaatacttc
     2161 agctgcgtca atacctttaa gtatcgatca agaattaaaa aatggtaaac tcaaagatga
     2221 tgatacaatt gttcttgtcg gattcggtgg cggcctaact tggggcgcaa tgacaataaa
     2281 atggggaaaa taggaggata acgaatgagt caaaatataa gagtagttat tacaggtatg
     2341 ggagcccttt ctccaatcgg taatgatgtc aaaacaacat gggagaatgc tctaaaaggc
     2401 gtaaatggta tcgataaaat tacacgtatc gatactgaac cttatagcgt tcacttagca
     2461 ggagaactta aaaactttaa tattgaagat catatcgaca aaaaagaagc gcgtcgtatg
     2521 gatagattta ctcaatatgc aattgtagca gctagagagg ctgttaaaga tgcgcaatta
     2581 gatatcaatg aaaatactgc agatcgaatc ggtgtatgga ttggttctgg tatcggtgga
     2641 atggaaacat ttgaaattgc acataaacaa ttaatggata aaggcccaag acgtgtgagt
     2701 ccatttttcg taccaatgtt aattcctgat atggcaactg gacaagtatc aattgactta
     2761 ggtgcaaaag gaccaaatgg tgcaacagtt acagcatgtg caacaggtac taactctatc
     2821 ggtgaagcat ttaaaattgt gcaacgcggt gatgcagatg caatgattac tggcggtacg
     2881 gaagctccaa ttactcatat ggcaattgca ggtttcagtg caagtcgagc gctttctaca
     2941 aatgatgaca ttgaaacagc atgtcgtcca ttccaagaag gtagagacgg ttttgttatg
     3001 ggtgaaggtg ctggtatttt agtaatcgaa tctttagaat cagcacaagc tcgaggtgca
     3061 aatatttatg ctgagatagt tggctatggt actacaggtg atgcttatca tattacagcg
     3121 ccagctccag aaggtgaagg cggttctaga gcaatgcaag cagctatgga tgatgctggt
     3181 attgaaccta aagatgtaca atacttaaat gcccatggta caagtactcc tgttggtgac
     3241 ttaaatgaag ttaaagctat taaaaataca tttggtgaag cagctaaaca cttaaaagtt
     3301 agctcaacaa aatcaatgac tggtcactta cttggtgcaa caggtggaat tgaagcaatc
     3361 ttctcagcgc tttcaattaa agactctaaa gtcgcaccta caattcatgc ggtaacacca
     3421 gacccagaat gtgatttgga tattgttcca aatgaagcgc aagaccttga tattacttat
     3481 gcaatgagta atagcttagg attcggtgga cataacgcag tattagtatt caagaaattt
     3541 gaagcataat tataaaaatc ttcagtaacg ttgttttagt tactgaagat tttttctgtt
     3601 tctttatact aagatgagcg acagcacaat cgtcataata aaatataaaa tatttattaa
     3661 taataagggt attatccatg tagaaacaaa gtaatgctct ttttttacct cttgtgggtt
     3721 gaaaaatgga tcatcagaga tagacttctt ctttttcgaa gatgacattt gatactttaa
     3781 tcttctaaaa ccataacttg tcgcatcaaa aatgccttct tgtacaagta aaatcaaaaa
     3841 aatgctaata aaaataatta atgaaacata aaacaatata tttaaatatg taatgatagt
     3901 atggctatta aaaacccata taataaacgt tagcattggg gttattagtg ccattccaag
     3961 ccattttttc aacatttgat cactcccact tatagaaaac tcttacgcat agtttacatt
     4021 aaaatgagac attgaggaat gattttttaa tttcttcagc tttattgaaa ttctaaaatc
     4081 aatcattctt cattagttta aagcaaaaaa atattgatat atagtaaata ttgtatatat
     4141 aatattagtt aagatttcag aaaattttga agggaatgga aatttagaaa tcggaatttg
     4201 ttagaggagg ggattagatg gggaaatata tttttaaacg atttatttat atgcttattt
     4261 ctttatttat tattattaca attacatttt tcttaatgaa attaatgcca ggttcaccat
     4321 ttaacgatgc taaattaaat gctgaacaaa aagaaatttt aaatgaaaaa tatggattaa
     4381 atgatcctgt agcaacgcag tatttacatt atttaaaaaa tgttgttaca ggggattttg
     4441 gtaattcatt ccaatatcat aatcaacctg tgtgggattt gattaaaccg agactattac
     4501 cttcttttga aatgggtctt acagcaatgt tcatcggtgt gatactgggt cttattttag
     4561 gtgttgcagc agctactaaa caaaattctt gggttgatta tacaactaca gttatttcag
     4621 ttattgcagt atctgtacca tcttttgtac ttgctgtact tttacaatac gtatttgcag
     4681 ttaaattaag atggttccca gtagctggat gggaaggttt ttcgaccgcg gtattaccgt
     4741 cacttgcatt atctgcagct gttttagcaa ctgtcgccag atacataaga gcagagatga
     4801 tagaggtatt aagttcagac tacattttat tagcgagagc taaaggtaat tcgacaatgc
     4861 gtgtactttt tggacatgca cttagaaatg ctttaattcc aattattaca attatcgttc
     4921 ccatgttagc aagtatttta acaggcactt taacaattga aaatattttt ggagttcctg
     4981 gattagggga tcaattcgta cgttcaatta caacaaatga tttctcagta atcatggcaa
     5041 tcacactatt atttagcaca ctgtttatcg tttctatttt tattgtagat attttgtacg
     5101 gtgtgataga tccacgaatt cgtgttcaag gaggtaaaaa ataatggctg aaaataaaaa
     5161 caatttgtcg attaacgacg atcattctaa tgcagctatg acgcatacct ctgacgctat
     5221 cgcatcatct gattttatta ttagagaatt agatttgaat caggaacctg aaatgcaacg
     5281 agaaagcaaa aacttttggc aagatgcttg ggctcagtta aaacgaaata agttagctgt
     5341 tgtcggtatg ataggtttaa ttatcattgt aatatttgct tttatcggtc cagttataaa
     5401 taaacatgat tatgctgaac aaaatgtaca acatagaaat cttccggcaa aaataccttt
     5461 attagacaaa gttccatttt taccttttga tggtaaagat gcagatggca aggatgctta
     5521 taaagcagca aatgctaaag aaaattattg gtttggtact gatcagttgg gtcgagattt
     5581 atggacaaga acatggaaag gtgctcaaat ttcattgttt atcggtgttg ttgcagcgat
     5641 gttagatatt tttattggcg ttgtatatgg tgcgatttct ggattcttcg gtggacgtgt
     5701 cgatacgatt atgcaacgta tacttgaagt catagcatct attccgaatt taattgtcgt
     5761 aattttattt gtattaattt ttgaaccatc catttggaca attatattgg ctatgtctat
     5821 cacaggctgg ttaggcatga gcagagttgt acgtggagaa tttttaaaat tgaaaaatca
     5881 agagtttgtc atggcttcga aaacattggg ggcttctaaa ttcaaattga tatttaagca
     5941 tattttacct aatacgttag gtgctatcgt ggttacatca atgtttacag tacctagtgc
     6001 tattttcttc gaagcatttt taagtttcat tggtataggt gtacccgcac ctcaaacatc
     6061 gttagggtca ttagtaaatg atgggcgcgc aatgctatta atttatccac atgaattatt
     6121 tataccagca atgattttaa gtttattaat tctattcttt tacttattta gtgatggatt
     6181 acgtgatgca tttgatccga aaatgcgtaa ataaaagggg gcatagcata tgactgaaag
     6241 aatattagaa ctaaatgatt tgcatgtttc ctttgatatt acagcagggg aagtgcaggc
     6301 agtgagaggt gtagattttt atttaaacaa aggggaaaca ttggcaattg ttggtgaatc
     6361 aggttcaggt aaatctgtga caacaaaagc aattacaaaa ttattccaag gggacacagg
     6421 aagaattaaa aagggagaaa ttttattttt aggggaagat ttagcaaaaa aacctgaaaa
     6481 tgagttaatt aaattacgtg gaaaagatat ttcaatgatc tttcaagatc caatgacatc
     6541 tttaaaccca acgatgcaaa ttggtaaaca agtcatggaa ccattaatta agcacaaaaa
     6601 ttatagtaaa gcacaagcta aaaagctcgc attggaaata ctaaatcttg taggattacc
     6661 aaatgcagaa aaaagattta aagcatatcc acatcaattt tcaggtggac aaaggcaaag
     6721 aattgttatt gcaaccgcat tagcttgtga acctaaagtg ctgattgctg atgaaccaac
     6781 gactgcatta gacgtaacga tgcaggcaca aattttagat ttaatgaaag aactacaaca
     6841 aaaaatcgat acagcaatta tttttataac gcatgattta ggggttgttg cgaatattgc
     6901 tgatagagtg gcagttatgt atggtggtca aatggttgaa acaggagatg ttaacgaaat
     6961 attttatgat ccaaagcatc catatacatg gggattatta tcgtcaatgc ctgatctatc
     7021 aacaacaaat gacacaccat tactagcgat tcctggagcg ccacctgatt tattacaccc
     7081 acctaaaggt gatgcatttg cgagacgtag tcaatatgca ttagatattg attttaaagt
     7141 agaaccgccg tggtttaaag tttcaccgac acattttgtg aaatcttggt tattagacgc
     7201 acgtgcacca aaagttgaac tacccgagct ggtaaaacaa cgtatgaaac cgatgcctaa
     7261 taattatgaa aaaccactca aggtagaaag ggtgtcgttc aatgaaaaat gatgaagtgc
     7321 tattatctat taaaaattta aagcaatatt ttaacgcagg aaagaaaaac gaagtgagag
     7381 cgattgaaaa tatttcgttt gaaatataca aaggagaaac attaggttta gtaggagaat
     7441 cggggtgtgg taaatctaca actggtaaat caattatcaa acttaatgat attacaagtg
     7501 gagaaatttt gtatgagggt attgatatac aaaagattcg taaacgtaaa gatttgctta
     7561 aatttaataa aaagatacag atgatttttc aagacccata tgcgtcttta aatcctaggt
     7621 taaaagtaat ggatatagta gctgaaggta ttgatatcca tcatttagca actgataagc
     7681 gtgaccgtaa aaaacgtgtc tatgatttac ttgaaactgt tggattaagt aaagaacatg
     7741 ccaatcgtta tcctcatgaa ttttcaggtg gacaacgcca acgtattgga attgcccgtg
     7801 cattagccgt tgaaccagaa tttattatag cagatgaacc tatttcagca ttggatgttt
     7861 caattcaagc tcaagtagtc aatttgttat taaaattaca gcgtgaaaaa ggtattacat
     7921 tcctatttat tgcacatgat ttatcgatgg tgaagtatat ctctgatcga attgcagtca
     7981 tgcattttgg gaaaattgta gaaatcggac cagcagacga tatttatcaa tatccattac
     8041 atgattatac taagtcttta ttgtcggcaa ttcctcaacc ggatcctgaa tcggaacgta
     8101 gtcgtaaacg atttagttat attgatgatg aagcaaataa tcatttaaga caattacatg
     8161 aaataagacc acagcatttt gtctttagta ctgaagaaga agcggcacga ctacgaaaaa
     8221 atttgttggt aacacaagat taaggggaag ggagaaatga aatgacaaga aagttaaaaa
     8281 cgctgatttt aatatttgtt gccacaattg cattaagtgg ttgtgctaat gatgatggta
     8341 tttattcaga taaaggtcaa gtattcagaa aaattttgtc agcagactta acatcacttg
     8401 atacatcatt aattacggat gaaatatctt ctgaagtgac tgcgcaaaca ttcgaaggtt
     8461 tatatacatt aggaaaaggt gacaaaccgg tgttaggtgt tgcgaaagct tttcctgaaa
     8521 agagtaaaga tgggaaaact ttaaaggtta aattaagaag cgatgctaaa tggagcaatg
     8581 gtgacaaagt gactgcacaa gactttgttt atgcttggag aaaaacagtt gaccctaaaa
     8641 ctggttctga atttgcatac attatggggg acattaaaaa tgcgagtgat attagtactg
     8701 gtaagaaacc tgtagagcaa ttaggtatca aagcattaga tgatgaaaca ttacaaattg
     8761 atttagaaaa gccggtacca tacattaatc aattattagc acttaataca tttgcacctc
     8821 aaaatgaaaa agttgccaaa aaatatggta aaaattatgg tacggcagct gatagagcgg
     8881 tatacaatgg tccatttaaa gttgatgatt ggaaacaaga agataaaacg ttactatcta
     8941 aaaatcagta ttattgggat aaaaagaaag taaaattaga taaagtgaat tataaagtta
     9001 ttaaagactt acaagcaggt gcatcattgt atgatactga atctgtagat gacgcagtta
     9061 ttacagcaga tcaagtaaat aaatataaag ataataaagg attaaacttt gtgttaacga
     9121 ctgggacatt ttttgtaaaa atgaatgaaa aacaatatcc tgattttaaa aacaaaaatt
     9181 taagactggc tatcgcacaa gcaatagata aaaaaggata cgttgattca gtgaaaaaca
     9241 atggttcaat tccttccgat acactaacag ccaaaggcat tgcgaaagca cctaatggca
     9301 aagattatgc gagtaccatg aattcacctc taaaatataa tcctaaagaa gcaagagcgc
     9361 actgggacaa agctaaaaaa gagttaggta aaaatgaatt gtcattttcg atgaacacag
     9421 aagatacacc agatgcaaaa atatctgctg aatacatcaa atcacaagtt gagaaaaatt
     9481 taccaggagt tactttgaaa attaagcaat tgccgtttaa acaaagagta tcactagaac
     9541 tgagtaacaa ttttgaagca tcacttagtg gttggtctgc agattaccct gatcctatgg
     9601 cttatttaga aacaatgacc acaggtagcg cacaaaataa tacagactgg ggtaataaag
     9661 aatacgatca attacttaaa gtagcgagaa ccaaattggc acttcaaccg aacgaacgat
     9721 atgaaaactt gaaaaaagca gaagaaatgt tcctaggaga tgcaccggta gcaccaattt
     9781 atcaaaaagg tgttgcacat ttaacaaatc ctcaagtaaa aggattgatt taccataaat
     9841 ttggtccaaa taactcactt aaacatgtat atattgataa atcgatagat aaagaaacag
     9901 gtaagaagaa aaaataattt gctttgtaaa ttagactgga gatatatctc cagtcttttt
     9961 gtgttgcaga aaaactttgg gaataaaaaa ttaaaataag tcgtttttta aattactgaa
    10021 attgattaaa tgcataaatg actgaatatt ctaaaaataa acttgtaata attttttcta
    10081 tgagtaaact aaaaagaaaa tattagattg aaagtaggag gcatatgtat ggggaagctt
    10141 attaaatata tttcaatact tcttattgtc gttttagtgt tgagcgcttg cggaaaaagc
    10201 agtaataaag atgaaggagt aaaagatgct actaaaacag aagcgtcaaa acataaaggt
    10261 ggtactctaa atgtagcgtt aacagcaccg ccaagtggtg tttattcttc gttattaaat
    10321 agtacacatg cggatgctgt agttgaagga tattttaatg aaaacttatt aacaa
//
LOCUS       DATTQF010000088        10368 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.088, whole genome shotgun sequence.
ACCESSION   DATTQF010000088 DATTQF010000000
VERSION     DATTQF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.088"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            126..959
                     /locus_tag="VB241_001840"
     CDS             126..959
                     /locus_tag="VB241_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP1245131.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAALIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1241..2038
                     /locus_tag="VB241_001841"
     CDS             1241..2038
                     /locus_tag="VB241_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245132.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2074..3078
                     /locus_tag="VB241_001842"
     CDS             2074..3078
                     /locus_tag="VB241_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1245133.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGIALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLISIALIIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADMIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3075..4091
                     /locus_tag="VB241_001843"
     CDS             3075..4091
                     /locus_tag="VB241_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1245134.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIILQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            4325..5293
                     /gene="dhaK"
                     /locus_tag="VB241_001844"
     CDS             4325..5293
                     /gene="dhaK"
                     /locus_tag="VB241_001844"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEP1245135.1"
                     /translation="MMKKLINKKETFLTDMIEGMLIAHPELDLIANTVIVKKAKKEQG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPIPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVETVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGHSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANEVIVMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            5335..5919
                     /gene="dhaL"
                     /locus_tag="VB241_001845"
     CDS             5335..5919
                     /gene="dhaL"
                     /locus_tag="VB241_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEP1245136.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVAKDDMDNQDFIKL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            5912..6274
                     /locus_tag="VB241_001846"
     CDS             5912..6274
                     /locus_tag="VB241_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEP1245137.1"
                     /translation="MPKIILVSHSKEIANGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEALTKLDDDALCFYDIGSSEMNLDMAIEMYDGNHHVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            6390..6887
                     /locus_tag="VB241_001847"
     CDS             6390..6887
                     /locus_tag="VB241_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245138.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            7343..8410
                     /locus_tag="VB241_001848"
     CDS             7343..8410
                     /locus_tag="VB241_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245139.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKKYHIFIVIMMIAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            8561..9604
                     /locus_tag="VB241_001849"
     CDS             8561..9604
                     /locus_tag="VB241_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1245140.1"
                     /translation="MNKDNKWTMITALFIAVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNVRIYQNLTYNRVYPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVVIGG
                     DSAGAQLASQFTAIQTNNQLRKAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELKVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            9932..10360
                     /locus_tag="VB241_001850"
     CDS             9932..10360
                     /locus_tag="VB241_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245141.1"
                     /translation="MTRSALKPFKNKRVMVTGRIHRVLFKNYLDRHSTFKPNVRILLK
                     DVIVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGNLVTEEAYIQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
BASE COUNT         3588 a         1423 c         1954 g         3403 t
ORIGIN      
        1 gctattcttc atataaatta gttaaatagt aaatctttgt tagaactctt tccatagagt
       61 tgtgacagta tttttgttgt gctactattt ttatagtcta ataaatatat aaaggggatg
      121 gtttcgtgaa taaaacggtt aaagatttaa tactagttgt cttaggttca tttatctttg
      181 ctgcaggtgt aaatgcattt attatttctg gtaacttagg tgaaggtggg gttacaggtt
      241 tagcaattat tttatattat gcgtttcata tttcaccagc catcactaac ttcttggtca
      301 acgcagcatt gattgccata ggttataaat ttttgagtaa gagaagtatg tacttaacta
      361 ttcttgtaac aattcttatt tcaatatttc tgagtttaac agaatcatgg caagtagaaa
      421 ctggaaacag cattgtgaat gccatttttg gtggtgtaag cgttggacta ggaatcggag
      481 taattatcct tgcaggcggt acaacagcag gtacaacaat tttggcgaga attgcaacga
      541 aatacctcga tgtaagcacg ccatatgctt tgcttttctt tgatatgatc gttgttgcaa
      601 tttcacttac agttattcca cttgataaag tattagtaac agtaatatca ctttatatag
      661 gaacaaaagt gatggaatat gtcatagaag gtttaaacac taaaaaagct atgacgatta
      721 tttcaactaa tcccgacaaa cttgccaaag caatagacga gcaaattgga agaggtttaa
      781 ccattttaaa cggacatggc tattatacac gtgaagaaaa agatgtctta tacgttgtta
      841 tttctaaaac acaagtatca aaagcaaagc gattaattaa acaaatcgat aaagatgcat
      901 tcctcgtaat tcatgatgta agagatgtct atggtaatgg ttttcttgca gatgaataaa
      961 taaatggtaa gagcacacat acttaaatag aagtccgcgg acaagttttt gaattatgaa
     1021 gacttatctg tggacgtttt ttattttata aaagtaatat acaagacatg acaaatcgag
     1081 ctatccaatt taaaaagtaa tgttagtcaa taagattgaa aaatgttata atgattttca
     1141 tgataatcat tatcaattgg gatgcctttg aaaattgata atttaaaaat agaaattatt
     1201 ttttataaac agaaagaatt ttattgaaag tagggaaatt atgaatcgtt tgcatggaca
     1261 acaagttaaa attggttacg gggataacac gattataaat aaattagatg ttgaaatacc
     1321 agatggcaaa gtgacgtcaa tcattggtcc taacggctgc gggaaatcta ctttgctaaa
     1381 ggcattgtca cgtttattgg cagttaaaga aggcgaagta tttttagatg gtgaaaatat
     1441 tcatacacaa tctacgaaag agattgcaaa aaaaatagct attttacctc aatcacctga
     1501 agtagcagat ggcttaactg ttggagaatt agtttcatat ggtcgttttc cacatcaaaa
     1561 aggatttggt agattaactg ctgaggataa gaaagaaatt gattgggcaa tggaagttac
     1621 aggaactgat acattccgac accgctcaat caatgattta agtggtggtc aaagacaacg
     1681 tgtttggatt gcaatggcat tagcacaaag aactgatatt atctttttag acgaaccaac
     1741 cacatattta gatatctgtc atcaattaga aatactagaa ttagttcaga agctaaatca
     1801 ggaacaaggt tgtacaattg tcatggttct tcatgatatc aaccaagcga ttcgtttctc
     1861 agatcatctt attgcgatga aagaaggcga tatcatcgct acaggttcaa cagaagacgt
     1921 attaacacag gaaattttag aaaaagtttt caatattgat gttgttttaa gtaaagatcc
     1981 taaaactgga aaacctttac tggtaactta tgacttatgt cgcagagctt attcttaatt
     2041 aagtaagtta atatgataaa aaggacaatt aacatgacaa atagagagaa tccaacgcca
     2101 ttgaagtttt tatcctatat tataggttta agtatgatac tactaatcac actttttatt
     2161 tctacattaa taggtgacgc caaaattcaa gcctctacaa ttatagaggc tatttttaat
     2221 tataatccta gcaatcaaca gcaaaacatc atcaatgaga ttaggattcc gagaaatata
     2281 gcagcagtaa ttgtgggtat agcgcttgca gtttctggtg cgattataca aggtgttact
     2341 cgtaatggtc ttgctgatcc ggcgctcata ggtttaaatt caggtgcttc atttgcttta
     2401 gcattaacat atgcagtttt accaaacact tcatttttaa tattgatgtt tgctggattt
     2461 ttaggtgcta ttctaggagg tgctattgta ttaatgatag gccgatctag acgtgatgga
     2521 tttaatccga tgcgtattat tttagcgggt gcagcagtaa gtgctatgtt aacagcgcta
     2581 agtcaaggta ttgcattagc ttttaggtta aatcaaacag taacattttg gactgctgga
     2641 ggcgtttcag gcacaacatg gtcacacctt aagtgggcaa ttccattaat tagtattgcg
     2701 ttaatcatta tattaacaat tagtaaacaa cttaccattt taaatcttgg tgaatcatta
     2761 gctaaaggtt taggtcaaaa tgtaacaatg atcagaggca tatgtttaat tattgctatg
     2821 attctagcag gtatagcagt tgctatcgct ggacaagttg catttgtagg attgatggtg
     2881 cctcatatag caaggttttt aattggaact gattatgcta aaattctacc attaacagcc
     2941 ttgttaggtg ggatactcgt gcttgttgct gatatgatag cacgatattt aggagaagcg
     3001 cctgttggtg caatcatttc atttatcggt gttccttact ttttatattt agttaaaaaa
     3061 ggaggacgct caatatgatt agttcaaata ataaacgcag acaatggata gcactggctg
     3121 tttttagcat tctactattt ctaggttgta cttggagtat tacctcaggt gaatacaaca
     3181 tacctgtaga aagatttttc aaaactttaa ttggacaagg tgatgccatt gatgaattaa
     3241 tcttattaga tttcagatta cctcggatga tgattactat tttggcaggt gcagcgctta
     3301 gtattagtgg tgcaatagtg caaagtgtca cgaaaaatcc aatagctgaa ccaggtatat
     3361 taggtattaa cgcaggtggc ggatttgcaa tcgcattatt tattgcaatt ggtaaaatta
     3421 atgctgacaa ctttgtttat gtactgccgt taataagtat actaggtggt atcaccgctg
     3481 cattgattat ttttattttc agttttaata aaaatgaagg tgttacacct gcgagtatgg
     3541 tattaatagg tgtaggttta caaacagcat tatatggtgg ctcaattaca attatgtcaa
     3601 aatttgatga taagcaatct gatttcatcg ctgcttggtt tgcaggtaat atttggggtg
     3661 acgaatggcc atttgtcatt gcatttttac cgtgggtgtt gattattatt ccttacttac
     3721 tatttaaatc gaatacacta aatattattc atacgggtga taatattgca cgaggtctag
     3781 gtgtaaggtt aagcagagaa cgtttaatat tattctttat cgcagtgatg ttatcatctg
     3841 ctgctgtagc agtagcaggt tcaatttcgt ttatcggatt aatgggcccg catattgcca
     3901 aacgtatcgt tggaccacgt caccagttgt ttttaccaat tgccatttta gtaggggcat
     3961 gtttacttgt tatagctgat acaattggca aaattatatt acaaccaggt ggggttccag
     4021 caggtatagt cgtagcaatt attggcgcac cgtatttctt atatttaatg tacaaaacga
     4081 aaaatgtata gtgtcaaagg acacaactta ttgctatgaa aggcacttta ttatatggct
     4141 tttcatagca ttttttattt aatgagtcac tcaagactat ttacattttc aataatgaac
     4201 cattaagtta tcaagactgt cttatcataa atatatttta taacggtatc aggttaattc
     4261 tttatgttag cgcattcatt tattctgttt tatactatga ctgacaatac caaggaggta
     4321 caacatgatg aaaaagttaa ttaataagaa agaaacattt ttaactgata tgatagaggg
     4381 catgttaatt gcgcatccag agttagatct gattgctaat acagttatag taaaaaaagc
     4441 taaaaaagaa caaggtgtag caatagtctc tggaggtgga agcggacatg aacctgcgca
     4501 tgctggattt gtagctgaag gtatgctaga tgcagcggtt tgtggcgaag tatttacatc
     4561 acctatacca gataaaatat tagaagctat taaagcagta gatactggtg atggtgtatt
     4621 actagttgtg aaaaactatg caggtgacgt gatgaatttt gaaatggcac aagagcttgc
     4681 agaaatggaa ggtataaatg ttgaaacagt tattgttcgt gacgacattg ctgtgacaaa
     4741 tgaagtacaa cgtcgtggtg ttgcaggaac agtgtttgtt cataagcttg ccggttatct
     4801 tgctgaaaaa ggtcattcat taacagagat taaatcgcgt gtagaagcgt tgttacctga
     4861 aattaaaagt attggtatgg caattgagcc accgcttgtt ccaactactg gaaaatatgg
     4921 ctttgatatt gaagacgaca aaatggaaat cggtattggt atacatggtg aaaaaggtat
     4981 tcatagagaa gaagtaaagg atattgatca tattgttgga acattattag acgaattgta
     5041 taaagaagta actgccaatg aagtcatagt aatggtaaat ggtatgggtg gtacgccgtt
     5101 atctgaatta aatatcgtaa ctaaatatat tcaacaaaat ttggctgcaa gaacggttaa
     5161 tgttgctaaa tggtttgttg gtgattatat gacatcttta gacatgcaag gtttttctat
     5221 aactatcgtg cctaataaac cagaatatct ggaagcattt ttagcaccaa caacaagtca
     5281 atactttaaa taagcagtga atatgaataa atatacattt atgaggtggc acaaatgaaa
     5341 gtgaatgata tgaaagcgcg tttattaaat ttagaagaaa cttttaaaaa acatgaatct
     5401 gaattaactg aattagatcg agcaattggt gatggtgacc atggggttaa catggttcgt
     5461 gggtttagta gtcttaaaga caaacttgat gatagctcaa tgcagtcatt gttcaaatct
     5521 actggtatgg cattgatgtc aaatgttggc ggtgcatcag gaccactgta tggctttagc
     5581 tttgttaaaa tgtctgcagt cgccaaagat gatatggata atcaagattt tattaaacta
     5641 attcaggcat ttgccgaagc ggttgaatca cgtgggaaag ttactttaaa tgaaaagaca
     5701 atgtatgatg tagtagcacg agcagcagag aagcttaaaa atggtgaaac tttaacattt
     5761 aatgatttac agcaattagc agataataca aaagatatgg tagcaacgaa aggtagagct
     5821 gcatattttg gagaagaatc aaaaggttat atagatccag gtgctcaaag catggtttat
     5881 attttaaacg ctttgattgg agatgaagat aatgcctaaa attatacttg ttagccacag
     5941 taaagaaatt gcaaatggta caaaatcttt gttaaagcaa atggctggtg acgttgatat
     6001 tataccaatc gggggattac cagatggttc aattggaact tcatttgata tcatccaaga
     6061 agctttgact aaattagatg atgatgcatt gtgtttttac gatattggat cttcagaaat
     6121 gaatttagat atggcaattg aaatgtatga tggtaatcat catgtgttaa aagttgatgc
     6181 gccaattgtt gaaggtagtt ttatcgcagc agtaaagctg tcaattggcg gttcaattga
     6241 tgatgcatta gcagaaatca aacaatcatt ttagttaaaa tttactaata atgaaaaatg
     6301 taaacctttt tcaaatgaaa ctttataaaa aatatgatag tatatatgta aatgtttaat
     6361 aaaatctgga gaaataggag gacattgcca tgcaacacct tataaaaaaa catgtattga
     6421 atggcgagtt tgatttagta cgacaattga tgtccgaaac agattttatg gaatttgaag
     6481 aagcatatat ttcaagtgcg catgaagtag aaagtatgat gttttataca tgtattttag
     6541 atatgattaa gtacgaagaa tcatctgaaa tgcatgactt agcattttta ttgcttgtgt
     6601 atccactaag tgaatatgaa ggtgctttgg attctgctta ttatcatgca gacgcttcca
     6661 taaaactaac tgacggcaaa gaagttaaaa gtttgttaca aatgttatta ttgcatgcga
     6721 taccaacacc tgttatttca gataagaagg cttttgatat cgccaagcaa attttaaaat
     6781 tagatcctaa taataatgtt gctcgtaacg tcttaaaaga caccgccaaa cgtatggaca
     6841 acgttgttgt tgatataaat gaattacacc aacgtaatgc acgttaatta catttcaatt
     6901 atattagctt aataatagtt ttaacatttg gttgggttgg gacatatgtt ccagcctttt
     6961 ttaatactta aaaactaacg aagtaaactt gtgtgcacaa atggttttta tacaacattt
     7021 tataaattta tacattttaa taaagaacat acgatagata gtttaaacct ttttaactga
     7081 gaaattttga tatgtatttt cgaaacttaa ctaaaaatac gtaagtcaag aagcacaatt
     7141 attaatcatt tttcctataa aaaagttcgt atgactgcat tataaaagca taaatttata
     7201 atttttttaa atgtcattga acgtgataat gtgaatggat tgagcaattt tgaaaaagtg
     7261 aaaaataacc tatgcgactt gcaattaatt ttcagtacgt tataatgcac actgtgcaaa
     7321 attaaggagg tctattattc acatgatgat gaataaagaa gcaacaaaaa ttggatttgc
     7381 ctacgtcggc attgtagtgg gcgcaggatt ttcaacagga caagaagtta tgcaattttt
     7441 cactaaatat ggcttgtggg cttatttagg tgtcattata tctggtttta ttttagcttt
     7501 tattgggcgc caagtagcaa aaattggtac tgcctttgaa gcgacaaacc atgaatcaac
     7561 attacaatac gtattcggtg aaaagtttag taaagtcttt gattatattt taatcttctt
     7621 cttatttggt atagctgtaa ccatgatagc aggtgcaggc gcaacatttg aagaaagtta
     7681 taacatacct acatggttag gtgcattaat tatgacatta gcaatttatg taacattgct
     7741 attagacttt aataaaatag tacgtgccct tggtgtcgtt acaccatttt taattgtttt
     7801 agttgtatta atcgctggcg tgtatttatt taaaggtcat gtttcattag cagaagttaa
     7861 ccaagtagtg cctgaagcaa gtatctggaa gggaatctgg tttggtacaa tatatggtgg
     7921 attagctttt tctgtaggtt ttagtaccat cgtagcaatc ggtggggata ctgaaaagcg
     7981 tacagtgtca ggtgcaggcg cgatgtacgg tggtattatc tatactgtat tactagcatt
     8041 gatcaacttt gcattgcaaa gtgaatatcc aactattaaa aatgcctcaa ttccaacatt
     8101 aacattagcc aataacattc atccgttaat agcaacagtg ttatctgtta ttatgctggc
     8161 ggttatgtac aatactattc taggactaat gtattcattt gcagcacgtt ttacagaacc
     8221 gtatagcaaa aaataccata tctttattgt tataatgatg atagcaggtt atttattaag
     8281 tttcgtagga tttgctgaat taattaataa gttatataca attatgggat atgtaggcct
     8341 atttattgta gtagctgtaa ttattaaata tttcaaacgt aaaaatgcgg ataaaaaaca
     8401 tattgcttaa tatcatatga tggatatccg aaactttaca attgaatcac tttgttttaa
     8461 tcttaaaagc aattcgtctc tactcttatc gggcgaattg ctttttatat ttattcagtc
     8521 tattaatatg agcgtctaac aaatagagag gtacgatgta atgaataaag ataataaatg
     8581 gacgatgata actgcgcttt ttatagcagt aatcagtgta ttgttagcat ttcatctgaa
     8641 acaacattat gaccaaacta caaatgagaa ccatgctaat aaagacaaaa ttaatattaa
     8701 aaataaaaat gtgcgcattt atcaaaacct tacatacaat agagtttatc ctaacagtaa
     8761 attagatatt attacacctg ttgatatgtc ttctaatgcc aaactgccag ttattttttg
     8821 gatgcacggt ggtggttata ttgcgggtga taagcagtat aaaaacccat tattagcgaa
     8881 aattgctgaa caagggtaca ttgttgtgaa tgtaaattat gcattggcgc cacaatataa
     8941 atatcccaca ccattaattc aaatgaatca agcgactcaa ttcattaaag aaaataaaat
     9001 gaatttacct attgatttta atcaagtagt tattggcggt gattctgctg gcgctcaatt
     9061 agcaagtcaa tttactgcaa tacagacaaa taatcaatta agaaaagcca tgaaatttga
     9121 tcagtcattc aaaccatcgc aaattaaagg tgctatacta tttggtggtt tttataatat
     9181 gcaaacagtt agagaaactg agtttccgcg aatacaatta tttatgaaaa gttatacagg
     9241 tgaagaagat tgggaaaaga gttttaaaaa tatttcacaa atgtcgacag taaaacaatc
     9301 gacaaaaaat tatccaccaa catttttatc tgttggagat agtgatccat tcgaaagtca
     9361 aaatatagaa ttcagtaaga aattacaaga attgaaagta ccagtagata cattgtttta
     9421 tgatggtacg catcatttac atcatcagta tcaatttcac cttaataaac ctgaatcgat
     9481 agataatatc aaaaaagtgt tacttttctt aagtcgtaat acatcttcta gtggtattca
     9541 aactgaagag aaaccacaaa tagaaaatcc gagtaatgaa ttaccgttaa atccattaaa
     9601 ctaatgataa acagtagtaa tttattactt aagcaacatt taagatcttc aaattgaaaa
     9661 cgaagaattt aaaacatgtg gggctaatgt gtaagtgtct gagatataat aaattgttag
     9721 cggttcttta tcatttctgt ccccccctat tatgatgtga catttattta caaactaatt
     9781 tgttttgaac ctgaaaataa ctttttacaa ctaaatagtc gaaaaacagt gtgtacatga
     9841 tataataaat ttataaattg aaaagaattc aaaagaaaaa ttaaaatata gattgagcac
     9901 gaagtgattt gaaataaggt tgtgaaaggg aatgacaagg tcagcattaa aaccatttaa
     9961 aaataaacgc gttatggtta ctggacgtat acatcgtgtg ttgtttaaaa attatttaga
    10021 tagacatagc acatttaagc cgaatgtaag gatattatta aaagatgtaa ttgtttcagg
    10081 tgtttcaata gatcatttat ggctatatga gacaaataaa tactatgcat tagcaatgga
    10141 acttattcat caacgagtaa aatttagtgc gaatgttgta ccgtattaca aaataaatag
    10201 aaataataat ttattcgtac aagattatgg aattaagcgt aaaggtaatt tagttactga
    10261 agaagcttac attcaaaaca atcagtatca ggataaaata tatgaaaaat taccggatat
    10321 agattttaga ctcgaagatt tttatagtaa ggaaaactaa ataatata
//
LOCUS       DATTQF010000089        10340 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.089, whole genome shotgun sequence.
ACCESSION   DATTQF010000089 DATTQF010000000
VERSION     DATTQF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.089"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            11..979
                     /gene="pfkA"
                     /locus_tag="VB241_001851"
     CDS             11..979
                     /gene="pfkA"
                     /locus_tag="VB241_001851"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP1245142.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQFINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            1001..2758
                     /gene="pyk"
                     /locus_tag="VB241_001852"
     CDS             1001..2758
                     /gene="pyk"
                     /locus_tag="VB241_001852"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP1245143.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIEEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNV
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(3105..4466)
                     /locus_tag="VB241_001853"
     CDS             complement(3105..4466)
                     /locus_tag="VB241_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1245144.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAYKTQFGHASFTNLYEHGVFPKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKTPDLHKKA
                     TYKLFGGKYMGYLIFVFFIFVFGLLFINVETRRAIYFIPIWFILLGFMYLRYKRIAAK
                     SNN"
     gene            4814..5935
                     /locus_tag="VB241_001854"
     CDS             4814..5935
                     /locus_tag="VB241_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEP1245145.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPIKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            5984..7252
                     /gene="icd"
                     /locus_tag="VB241_001855"
     CDS             5984..7252
                     /gene="icd"
                     /locus_tag="VB241_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEP1245146.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVEKEGRDAANVAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            7642..8343
                     /locus_tag="VB241_001856"
     CDS             7642..8343
                     /locus_tag="VB241_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1245147.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKE"
     gene            8346..10007
                     /locus_tag="VB241_001857"
     CDS             8346..10007
                     /locus_tag="VB241_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEP1245148.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDKKVFSSGKLKPTKTIDNYSQKKDI
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTEIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         3718 a         1445 c         1996 g         3181 t
ORIGIN      
        1 gtatgtcgtc atgaagaaaa ttgcagtttt aactagtggt ggagattcac ctggaatgaa
       61 tgctgccgta agagcagttg ttcgtacagc aatttacaat gaaattgaag tttatggtgt
      121 gtatcatggt taccaaggat tgttaaatga tgatattcat aaacttgaat taggatcagt
      181 tggggatacg attcagcgtg gaggtacatt cttgtattca gcaagatgtc cagagtttaa
      241 ggagcaagaa gtacgtaaag ttgcaatcga aaacttacgt aaaagaggga ttgagggcct
      301 tgtagttatt ggtggtgacg gtagttatcg cggtgcacaa cgcatcagtg aggaatgcaa
      361 agaaattcaa actatcggta ttcctggtac gattgacaat gatatcaatg gtactgattt
      421 tacaattgga tttgacacag cattaaatac gattattggc ttagtcgaca aaattagaga
      481 tactgcgtca agtcacgcac gaacatttat cattgaagca atgggccgtg attgtggaga
      541 tctagcatta tgggctggat tatcagttgg tgctgagaca attgtagttc cagaagtgaa
      601 aacagatatt aaagaaatag ctgataaaat tgaacaaggt attaaacgtg gtaagaaaca
      661 ctcaatcgtt cttgtagcag aaggttgtat gactgcgcaa gattgtcaaa aagaattatc
      721 acaattcatc aatgttgata atagagtgtc tgtgttaggt cacgttcaac gtggtggtag
      781 cccaacaggt gcggatagag ttttagcatc acgattaggt ggatatgcgg tagacttatt
      841 aatgcaaggt gaaacagcta aaggtgttgg aattaagaac aataaaattg tagcaacatc
      901 ttttgatgaa atttttgatg gtaaagatca taaatttgat tatagtctat atgaacttgc
      961 taacaagtta tctatataag atttcaggag gaattataaa atgagaaaaa ctaaaattgt
     1021 atgtacaatt ggaccagctt cagaatcaga agaaatgatt gagaaattaa tcaatgctgg
     1081 tatgaacgtt gcacgattaa acttttcaca tggtagtcat gaagagcata aaggtagaat
     1141 tgatacaatt cgtaaagtag ctaaaagatt agacaaaatt gtagcaattt tattagatac
     1201 gaaaggtcca gaaattcgta cgcataatat gaaagacggt atcattgaac ttgaacgtgg
     1261 taacgaagtt attgttagca tgaatgaagt tgaaggaaca cctgaaaagt tctctgtaac
     1321 ttatgaaaac ttaattaacg atgttcaagt aggttcatac attttacttg atgatggctt
     1381 aattgaatta caagttaaag atattgacca tgctaaaaaa gaagttaaat gtgatatttt
     1441 aaactctggt gagcttaaaa acaaaaaagg tgttaactta cctggtgtaa gagttagttt
     1501 acctggtatt acagaaaaag atgctgaaga tatccgtttc ggtattaaag aaaatgttga
     1561 cttcattgca gcaagtttcg tacgtcgtcc tagtgatgtt ttagaaattc gtgaaatttt
     1621 agaagaacaa aaagctaaca tttcagtatt ccctaaaatt gaaaaccaag aaggtattga
     1681 taatattgag gaaattcttg aagtgtctga tggtttaatg gttgcacgtg gtgacatggg
     1741 tgttgaaatc ccacctgaaa aagtaccaat ggttcaaaaa gatttaatca gacaatgtaa
     1801 caaattaggt aaaccagtta ttacagctac acaaatgtta gattctatgc aacgtaaccc
     1861 acgtgctaca cgtgcagaag ctagtgacgt tgccaacgca atctatgatg gtacagatgc
     1921 agtaatgtta tctggtgaaa ctgctgctgg tttatatcct gaagaagctg ttaaaacaat
     1981 gagaaatatt gctgtatcag ctgaagcagc ccaagattac aaaaagttat tgtcagatcg
     2041 tactaaatta gttgaaactt cattagtgaa tgctatcggt atttcggttg cacatacagc
     2101 tttaaactta aatgttaaag caattgtagc tgctactgaa agtggttcaa cggcacgtac
     2161 tatctctaaa tatcgtccgc attcagacat tattgcagtg actccaagtg aagaaactgc
     2221 acgtcaatgt tcaattgttt ggggtgttca acctgtagtt aaaaaaggac gtaagagtac
     2281 agatgcattg ttaaacaatg cagttgcaac agctgttgaa actggtagag taactaatgg
     2341 tgatttaatc attattactg ctggtgtacc aactggtgaa actggaacta ctaatatgat
     2401 gaaaatccac ctagttggtg acgaaattgc taatggtcaa ggtattggac gtggatcagt
     2461 tgttggtact acgttagttg ctgaaactgt taaagattta gaaggtaaag atttatctga
     2521 caaagttatc gttacaaact caatcgatga aacgtttgta ccttatgtag aaaaagcgtt
     2581 aggcttaatt acagaagaaa atggaattac atcaccaagt gcaattgttg gtttagaaaa
     2641 aggtattcca acagttgtag gtgtagaaaa agctgttaaa aacataagca ataacgtgtt
     2701 agttacgatt gatgctgctc aaggtaaaat ctttgaagga tatgcaaacg tactataatt
     2761 tataaaaaac gtctttccat ttatcaacaa tggaaaggcg ttttttggtt catctggtat
     2821 tttatgacgt aattaatagg atatttgata atgataatgt atgaatagca atctatataa
     2881 atgtttatat cttttataca tgcacattat cagctttaaa ctttcactca tattactttg
     2941 gaaagttatt ataaaataga agtatggatg tatctttaaa atgatacatt attcaataga
     3001 tgagaaagta aaagtattga ggcaagtacg caatttaata ttataagttg catataaaaa
     3061 cccctgttga actttaatat attcaacagg ggtaaaactt gttgttagtt atttgattta
     3121 gcagcgatac gtttatatct taagtacata aatcctaata gtataaacca aatcggaata
     3181 aagtatatcg cacgtcttgt ttcaacattg ataaacaata ggccaaacac aaatataaag
     3241 aatacaaaaa tgagatagcc catatatttt ccgccaaata atttatacgt agccttttta
     3301 tgaagatctg gagttttacg actataattg atataagcga tgataatcag tccccaaaca
     3361 actaagaata atactgtaga gattgttgtc acatacgtaa ataccttagt agcatcaggg
     3421 aaaatatagt ttaataatgc tgccacgagt aacaatgctg atgaagcaaa gatcgctaca
     3481 tgtggaacgc catacttatt cgtcttagag aaattcggtg gtgcttgttg ttgacttgat
     3541 aaaccgaaca gcatacgact attcgagaaa ataccactgt tacatgatga agcagcagca
     3601 gttagtacta caaaattaat caagcctgca gcaaatggaa ttccaattaa tgcgaataat
     3661 tttacgaatg gactgttatc aggatcaact tgttgccaag gaataatcga catgataacc
     3721 gctagagcac cgacataaaa tattaaaatt ctaataggaa cactattaat cgcttttggt
     3781 attgttttaa caggatcttt cgtttcacct gccgtaacac caatcatttc gattcccacg
     3841 aatgagaata aggccatttg gaatgacata aagaatccag atgctccttt agggaataca
     3901 ccgtgctcgt ataggttcgt gaaactcgca tgtccaaatt gagttttata tgcaaataga
     3961 atcataacga aacctacgac aattaaacca atgattgttg ctattttaat gatagagaac
     4021 caaaattcta actctccaaa tagtctcgca ctcaataagt taaaagacat taataacaat
     4081 acgcaaaaaa gtgcactaat ccagtttgga atttctggga accagaagct tacatacttt
     4141 gctacagctg ttacttcagc catacctgta ataatccaac agaaccagta tgtccaaccg
     4201 gttacgaatc ctgcaaaagg cccaatgtat gtattggtta catctgcgaa agatttaaat
     4261 tcagtatttt gaatgatgat ttcacctaaa cctcgcataa acataaataa cataaatcca
     4321 ataatgatat atgttagtaa tatagaaggg ccagttaatg caatcgtttg accagcgcct
     4381 aagaatagcc ctgtaccaat tgcgccacca atagcaatta attgaatgtg gcgattgctc
     4441 agttcccttt gtaatttttc agccataata catctccctt aaatatagat atgtttatta
     4501 tgcacttata ttaggatata tacaattatt ttcggtaaaa atgtgtaaaa ttccatgtta
     4561 atatactttg gattttataa tcatatataa taaccaattg aaaattttat tctatgctaa
     4621 aagttggtga tgaatataat cttgaaaaag ctttaatggt gctatttgtg gccattctgt
     4681 gacatttaca tagatttaca aaataattgt tgcacatata atgccagttt ttatatttca
     4741 caaacgaaat gcgtttagct ataatattag ttgaaagcca tttcataaag aaacagtaaa
     4801 ggggaaattt atcatggcag aattacaaag aggtttagaa ggggttatcg cagcggagac
     4861 taaaataagt tcaattattg aaagtcaatt gacttatgcc ggctatgata ttgatgatct
     4921 agctgaaaat gcgcaatttg aagaagttat tttcctatta tggaactata gattgccaaa
     4981 tgaagaagag cttgctcatt taaaggggaa attaaatcaa tacatgacat taaatcctcg
     5041 tgtgtataca cattttgagg agtatgttac agatcacgtg catccaatga cagcattacg
     5101 tacgtcatta tcatatattg cacatttcga tcctgatgct gaaaatgaat cagatgaaaa
     5161 tcgttatgaa agagcaatgc gtatacaggc taaagtagca tcattagtta cagcgtttgc
     5221 tcgagtaaga caagataaag aaccaattaa gcctaatcct gacttaagtt atgcagcaaa
     5281 cttcctatat atgttacgtg gggaattacc aacagatata gaagtggaag ccttcaataa
     5341 agcgcttatt ttacacgctg atcatgagtt gaacgcatct gcatttacag cacgttgtgc
     5401 ggtatcttca ttgtcagata tgtactcagg tattgtagca gctgtaggtt ctctgaaagg
     5461 gccattacat ggtggtgcaa atgaacaagt tatgacgatg ttatctgaga ttgggtcaat
     5521 tgaaaatgtt gatgcttact tagatgaaaa atttgctaat aaagataaag taatgggctt
     5581 cggtcatcgt gtatataaag atggtgatcc tagagcgaaa tatttaagag aaatgagccg
     5641 tcaaattacg aaagacgctg gtcgtgaaga attatttgaa atgtcagtga aaatggaaaa
     5701 acgcatggca gaagaaaaag gattaattcc aaatgttgat ttttatagtg cgagtgttta
     5761 tcactgtatg gaaatacctc atgacttatt cacgccaatc tttgctgtaa gtcgttctgc
     5821 aggatggatt gctcatattt tagaacaata taaagataat agaattatgc gtcctagagc
     5881 aaaatatatt ggcgaaacga atcgtaagta tattccgctt gaagaaagaa aataatcaat
     5941 acaaattaaa aatgaagatg taaaatttgg aggtaaaata actatgactg cagaaaaaat
     6001 tactcaagga actgaaggat taaacgtacc taatgagcca attatcccat ttattatcgg
     6061 tgatggaatt ggaccggata tttggaaggc agcaagccga gttatagatg ctgctgttga
     6121 gaaagcctat aatggcgaaa aacgcattga atggaaagaa gtgctagctg gtcaaaaagc
     6181 atttgataca actggcgaat ggttacctca agaaacactt gatacaatta aagaatattt
     6241 aattgctgtt aaaggacctt taacaacacc aattggtggt ggtattagat cattaaatgt
     6301 agctttacgc caagaattag atttatttac ttgtttaaga ccggtacgtt ggtttaaagg
     6361 agtaccatca cctgttaaac gtccacaaga tgttgatatg gttattttcc gtgaaaatac
     6421 tgaagacatt tatgctggta ttgaatttaa agaaggaaca acagaagtta aaaaggtaat
     6481 tgatttctta caaaacgaaa tgggtgcgac aaatattcga ttcccagaaa cttcaggtat
     6541 tggtattaag ccagtttcta aagaaggtac tgagcgatta gttagagcag caattcaata
     6601 tgctatcgat aataaccgta aatcagttac tttagttcat aaaggtaata ttatgaaatt
     6661 tacagaaggc tcatttaagc agtggggtta cgatttagca ttaactgaat ttggtgatca
     6721 agtatttact tggcaacaat atgacgaaat tgttgaaaaa gaaggcagag atgctgctaa
     6781 tgttgcacaa gaaaaagctg aaaaagaagg caagattatc attaaagatt ctattgctga
     6841 cattttctta caacaaattt taactcgtcc agctgagcat gatgttgtag caactatgaa
     6901 cttgaatggt gactacattt cagatgcatt agctgcacaa gttggtggta ttggtattgc
     6961 gccaggtgca aacattaatt atgaaacagg tcatgctatt tttgaagcaa cacatggtac
     7021 agctccaaaa tatgcaggtt taaataaagt gaatccatct tcagtaattt taagttctgt
     7081 attaatgtta gaacatttag gatggcaaga agcggcagat aagattacag attcaattga
     7141 agatacaatt gcttcaaaag ttgttactta tgactttgcc cgtttaatgg atggtgctga
     7201 agaagtttct acatcagcat ttgcagatga attgattaaa aatttaaaat aagcaaaata
     7261 aacttaggtg agtctagcaa ctttaattgc taggttcacc tttttaaatg aattgaagtt
     7321 ggcagcagga tagaaatgat aaagagtcac gaatgattga ttatgtagtg tttcttacac
     7381 attaactaaa gcgaatgtgt atataagctt aggaataaat gagtaaaact catgcatagg
     7441 cagtgggata gaaatgatat tttcacaaaa tttatttcgt tgtcccagag tatgtcatgc
     7501 aaattataga aggaaataaa agaagtattt tatttatgtg taaagtaatg taaaaaatgt
     7561 gtaaagataa tgtgtaggat gttattttta taaatagttc ggttataatg taaatataat
     7621 tataagttag ggaggcatac catgtcgcaa aaagtgttgg tagtagatga cgaacattca
     7681 atcgtaacat tacttaaata taacttagaa acagctggtt atgaagttgt tgtcgcattt
     7741 gatggtgatg aggctttaga gaaggtagaa aatgaacagc cagatttaat tattttagat
     7801 gttatgctac ctaaaaaaga tggcattgac gtatgtaaga ctgtaagaac aaataaaaat
     7861 ttagtcccta tattaatgtt aacggcgaaa aatgatgaat ttgatcgggt attaggttta
     7921 gaattaggtg ccgatgacta tatgactaag cctttttcac ctagagaagt tgttgcccgt
     7981 gtgaaagcca ttttaagacg ttctcaattt gtaaaagaag tagaagagat tgacgatgat
     8041 attaccatcg gatcgatacg tattagaccc gattattttg aagtatataa gcataatgag
     8101 ctattggagc taacaccgaa agaatttgaa ctgttgcttt atttgataga aagacaaggc
     8161 agagtcatta cgagagagca tatgttaaat acggtatgga attatgaatt tgcaggagac
     8221 tcaagaattg ttgatgtaca tatcagtcat ttaagagata aattagaaga taatcctaag
     8281 aagccacaac ttattaaaac tgtgcgtggc ttaggctata aattggaaag acctaaagaa
     8341 taatgatgaa gtttcaccac cgcttaatgt tattgataag cagtatatta attatcagtt
     8401 ttgttacatt aggcattatt attcacgctt ccatcacata tgttttatca gaaaaacacg
     8461 aacaagctct agcaacagat gcaagatcat atatttattt agtccaagat aatgagattg
     8521 ataaagttaa agaaattgta aaacagcaaa atattgattt attgataact aaaaatgata
     8581 aaaaggtgtt tagtagtggc aaactgaaac ctactaaaac aattgataat tacagtcaaa
     8641 aaaaggacat ttttattttt aataagaagg ttaacggtta tcatgtatgg attaaaggtt
     8701 acaataacga tattacagaa atgcattgga cgttgtggaa atatcttata ttgacatgtt
     8761 tagttgtact tatttgtctg tattttgcaa gccgtagttt taagcgtact ttaatccgtc
     8821 ccattcaaga agttacttat gcaacacaat tgttggcaaa tggttattat cacatacgtg
     8881 tacctgaaag taatgttgta gaaactaaag cgctttttgt gtctaccaat gatttagcac
     8941 gacgccttca aaagcttaat aatgagcaaa aaattcaatc caaccgttta aaaacaacga
     9001 ttgaaaatat tccaagtgct attttaatga tagatcgaaa tggaaaaatc gttgttgcaa
     9061 ataaagcgta ttatgaacag ttcaacatct cgcataatat tgaacaagta ggctatcacg
     9121 gctatgttaa tactgaaatt gagcaattaa ttttagaaag ttttaaagtt gaaaaaccta
     9181 tctatgaaca attggaagtt gcaataaacc aagtccatgc caagtacttt gatatttctt
     9241 gtgttccgat tttaactaga tctcaaaaaa gtttgcaagg gattttagtt gtgatgcatg
     9301 acataacaaa tctgaaacaa cttgaaaatc taagacgtga atttgtagca aatgtttcac
     9361 atgaattaaa aacacctatt acttcaatta aggggtttgc tgaaacactt attgatggtg
     9421 caaaaaatga tgcggaatca ttagacatgt ttttgaacat aattttaaaa gaatcaaatc
     9481 gaatagaatc tttagtaaca gatctgttag atttatcgca tattgagcaa cataccgagt
     9541 tagatacaga ctatatgaat ttatcagatt taacgcggcg tattattgat aatatgatga
     9601 ctcaagccaa tcaaaaaaat atttccattc atactgaaat tgaaaaagat gtcattgtta
     9661 aagcacaaga aagtaaaatt gctcaagtta tcacgaattt gttaacgaat gcaattaact
     9721 attcttatga agatggagat attaacgttc gtgtgtatcg agatgacttt cgtgtcattt
     9781 tcgaagtaca agattttggt ataggtatta aattagaaga ccaacaacgt atttttgaaa
     9841 gattttatag agtagataaa gcacgaagta gagattctgg tgggacaggt cttggattat
     9901 caattacaaa gcatatagta gaggcccacc aaggcaatat tgaagtgaat agtcaagttg
     9961 gcaaaggctc aacgttcaaa gttattctaa aagattataa agaataaaaa ttaaaagtgg
    10021 taacagcgcg tgtatttttc acgaagttgt tgctgctttt ttatttcgtt caaatctcta
    10081 ttggtattaa attagagtta atgaagtgga atatattcgg ttgtcaattt tgtcatttgc
    10141 atatgtcgtt ttgtaagtta taagtaatag attgttgtga atcaagcaat tttataatat
    10201 atttattata ataaagaaat tttagtaaca cgcttaaaat agatgcttaa ttaaaaagta
    10261 ataagtttta tttgaacgga aataaatctt aatttaaaca cttgaataca caagtgtaat
    10321 cattcgtcat agaaaaagac
//
LOCUS       DATTQF010000090        10194 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.090, whole genome shotgun sequence.
ACCESSION   DATTQF010000090 DATTQF010000000
VERSION     DATTQF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.090"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(178..1056)
                     /locus_tag="VB241_001858"
     CDS             complement(178..1056)
                     /locus_tag="VB241_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEP1245149.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1076..4516)
                     /locus_tag="VB241_001859"
     CDS             complement(1076..4516)
                     /locus_tag="VB241_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEP1245150.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFATFKSRVEKSIA
                     DWDIKLEHTFYVSKFDHSENELGALSTYLVSLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGLFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLVDRQETTYQVATDNAKDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEDFINSDLEKLKSHLNKNQ
                     LAFVHAVEKHYNLYVSMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDHSYVEMITEFETNKADISRRQHRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDIKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFKNQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4820..6161
                     /locus_tag="VB241_001860"
                     /pseudo
     CDS             4820..6161
                     /locus_tag="VB241_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
     gene            complement(6506..6838)
                     /locus_tag="VB241_001861"
     CDS             complement(6506..6838)
                     /locus_tag="VB241_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HEP1245151.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIQDMHMN"
     gene            complement(6922..8067)
                     /locus_tag="VB241_001862"
     CDS             complement(6922..8067)
                     /locus_tag="VB241_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP1245152.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(8276..8655)
                     /gene="rnpB"
                     /locus_tag="VB241_001863"
     ncRNA           complement(8276..8655)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VB241_001863"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(8718..9062)
                     /gene="gpsB"
                     /locus_tag="VB241_001864"
     CDS             complement(8718..9062)
                     /gene="gpsB"
                     /locus_tag="VB241_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP1245153.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(9076..9639)
                     /locus_tag="VB241_001865"
     CDS             complement(9076..9639)
                     /locus_tag="VB241_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP1245154.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(9632..9982)
                     /locus_tag="VB241_001866"
     CDS             complement(9632..9982)
                     /locus_tag="VB241_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HEP1245155.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     LLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
BASE COUNT         3166 a         1756 c         1457 g         3815 t
ORIGIN      
        1 ggatgaaata taattcttta tactctattg attaatcaaa tatgtatcta tcaaaattcg
       61 gcttatttat tcactctgac atattcaaat acgcaaaaag actatttcta ccttgtatcg
      121 atagaaatag tctttttata cttaattggt ttaatttttc ataaaatctt atacttttta
      181 aaaatgggat gaaatatatt ttcctgaaac atgtaattca tgttcgttac aaattgataa
      241 aatatgatta agcgttgttg caaatgacat tttctcaaaa agcgcttcac tatcaattgg
      301 tacttgtgtg tgaatttcag caagtcgctt cgataaatat agttcatcta aattgtcatt
      361 tatcttatta cgttgaccag ctgataatgc atcaatgttt tcaaccacat tttcaacgct
      421 ttgatattgc tgaattaact taatagccgt tttctcgcca atacctttaa cacctgcata
      481 tccatctgct gtatcaccca taaacgcttt aatatcgatt agttgttgtg gttcaagagc
      541 atattcttcg ttaaaacgat gtaatgtata tctattataa atgttaaaac cttttttaat
      601 tagccagact tcaacattgt cattaataca ttgcagtaaa tctttgtcgc ccgtaataat
      661 atagacatcg ttatcagttg aatattgttg tgctaatgta cctataacat catccgcttc
      721 atagttttta acgccaatat ttacaaagcc aaattgctct gaaatttctt taacataatc
      781 aaattgtggt atcaattctt ctggtggtgc agaacgattt tgcttataac cgtcaaacat
      841 atcattccta aaagttgatt gccccatatc ccaacataca gctacatgtg tagggcgtat
      901 ttcatgtatt gccgaaaaga tatgacgcac aaatccttgt attccatttg taggtacacc
      961 ttgtgaattg tacataaatt gtttatgaag acttgtagcg tagaaatgtc taaataatag
     1021 cgccatacca tctacaagta atattttatt tggcatttaa aacgttcctt ctttcttaat
     1081 ataaaatagt tttcagttgt tgatgtttat ctttcaattg attgataagt gttgtatcta
     1141 aatcaaatgc taataaatcg tttatttgcg tttgaatatt agcttcaaaa ctttcaaatt
     1201 gcgattcagc ttctacagcc atttgcttta catataattc aagctgttgc ctcaataatc
     1261 ccatttgtgg ttgtaataat cctaacgtac tttgacaaat tgattcatgt atatctcttt
     1321 gcccatttgg attcaaaatt ttacgttttg ttaattgttt aggcaatgca tttagcatat
     1381 cgttgaaatc aatgtgcaat aatggttgct ctatattagc tgattcaaag ttaaactgag
     1441 gattaacaat gacatgtaaa tctgctaatt gttgaacgat tggtgcaatt tgctcagtga
     1501 gttgattttt aaagtatttt tttatacgtt ctgtaattaa agattgctct aaaaacaatc
     1561 gttgatgaat ttgatctaag tatactttcg tagacacttt cttttcttca ttaaaatcac
     1621 tattttgcgt catttgagaa ttaaacactg atttaatatc gtctagtagt tgtagtttta
     1681 aacgggcatt taaatgataa acttgttctt caacttcgtt gtctgtatgt tgtagcgtcg
     1741 catcaattaa ttgatgttgt aaacgctgtt tatctttata gactgttaac ctatgttggc
     1801 gacgtgaaat atcagcttta tttgtttcaa attctgtaat catctctaca taagaatgat
     1861 ccatttgttg aagctgatga atcatttgtt gttctaaaat tgatttagat tcaacatcaa
     1921 caaattgttg tatgctttgt tttaattgat caatgccctt atcttcagct tgcaatgcat
     1981 ttcgacttga tacagcaaaa atgtctgatt gtaagtgtac ttgtcttaat gcatctgata
     2041 catatgtttc aaccgcttca agatcatctt gactttctgc taaatcagca gcattaatta
     2101 ccattttaaa tgcttggttt tcattcaact ggttcatatc tttcatgtgt tctataaacg
     2161 ccttgtcatt atcagtaaac gaatgattaa aataacttac atacaagatt aagtctgaag
     2221 aagttaaaat ttgctcggtt tcatttgtat gcctttggtt atttgagtgt agccctaatg
     2281 aatcaacaat tattttacct tttaaccaat catgcattaa tgcaatgtgt actgttttaa
     2341 caaatgttgc gtattcatct tctgcactcc actttttcaa ttcctgttga ttaatggcat
     2401 gtttttctcc attttccaac atactgacat acaaattata atgtttttca actgcatgaa
     2461 caaaagcgag ttgattttta tttaaatgtg attttaactt ttctaaatct gaattaataa
     2521 agtcttctat agttgaaaaa gatatatctt ggtattcaac tactgcatta atttcatcta
     2581 ataattgcga ttgtgatttt agcgttatat aactctcgtc tccataagat atttctgtag
     2641 tagctgctgt agtaggattt ggagaactaa ctaaaatatg ctcgcctaat aatgcattta
     2701 tcaaactact tttacccgca ctaaacgttc caaatacacc tatttttatt aatttattat
     2761 ctatacgtgt taatgtttcg tggatatcct gctttgtacg cttgaacaaa ggcacatccg
     2821 aaattatatc aagtgctttt tgaatatcta tagacatatt tgttgtagct gtattctgat
     2881 ttagctgctg attatcacga ttatccttag cattatcagt agccacttga tatgttgtct
     2941 cttgtcgatc tactaattta tctagagatt catctaaatg aatatagtaa tgacgataat
     3001 tcttagtcgt caatgactga cgcagctcat ttaattctgt ataacgttga tattctttta
     3061 aatcatcact ttcttctgtt ggtaattcat ctgcctgcac attttctatt atttctttaa
     3121 aaattggtgt tgattgttgt tcaacatatt tcttaatggc tttaaccact tcgtctgaaa
     3181 atgtaagtac ataagtattg ctgattgatg tttgtggttg atataaacct tcaatcattt
     3241 ccggcttaac gtcataatgc tgatttaata ctttatctga agcttctttt ttattgataa
     3301 aacgcgtaac aaatgacata tcttctcgca ttggttgacg aatttgttga ttaacatgtt
     3361 cttgtaacgc atctgtcgct gttaataatc gctgttgttg aatttgaagc tttttcttct
     3421 ttttattaaa aaggccacca acattaaagt cttgagacat actttccaaa taacttcgta
     3481 acatttctct catattatgc ggcataatgt atgcattttc taaaatattt ttacgcttat
     3541 tctttaaaaa tgccattaat tcatctggat tatttaaaag ttgcgcctct tcactaattg
     3601 cttgatgttg ttgactattt aaaaatgctt gttcaaactc tgcttcttcg atacccaaat
     3661 cttctagtac ttcctgaatt tcagactgaa tgtaatctag ctgagcttcg gtaatatatt
     3721 caaccgttct tgatgtataa tcctctattg tttctctatg ttgatctaat gaaactagat
     3781 aagttgataa tgctccaagt tcattttcag agtgatcaaa tttcgataca taaaaggtgt
     3841 gttctaattt aatgtcccaa tctgcaattg atttttcaac tcgagattta aacgtagcga
     3901 atgacaattc atcgtcttga tgcttgtcaa tttgattaat gataaacaca acaggtattc
     3961 caacatcatt tatatgcttc ataaacttaa agttaagttc agattgaacg tggttatagt
     4021 caaccgtata aaatatcata ttacttgtat acatatattg ttctgttatt gactgatgtg
     4081 atgcaacatt tgaatcaaca cctggtgtat cttgcaacgt aaacccattt tcaaatttag
     4141 ctgattgaaa attaatttct acagattcaa cgtcgacatt ttggcgattc atttccctta
     4201 cttcatcata attagataat ttggcatacg tttgattcgg caaattagca ataatatcgt
     4261 gattgtctga aactgacacg atagcagtat tacttgtcgt tggtacaggt gaacttggta
     4321 agatatcttg ttcaattaat aaatttatca gtgtcgattt acctgcagaa aaatgtccaa
     4381 cgaacgaaca tgtatattgc tgcaaatata ctttcttaat tacttggtta attgtatgta
     4441 aaagtgcttc atttcgcgac ttttcaactt ctttttttaa tttatataaa agatctaatt
     4501 gttctttatt aatcatgtta ctttcccctt tggttcgttc acatagaaat gcttgcttgt
     4561 taaaacgaat tcatacaata tcgatgtcca tttaaacaaa ggtcgttgaa tggctcgcat
     4621 tgtattatgt ttaaatttta tcatagctgc ttgataaaac atatgaataa tcaattttct
     4681 tcaaacattc caacttaaaa gttcattttt ctaaaaatca caaatcattg ttaaattttt
     4741 ctagaaaata aatattgtca cagtttgttc acacacgtca cagaattgac accgtataat
     4801 agccaattgg gagggaaata tgaaaaatac atttaatcca ttacaaagat tacatttcta
     4861 cgcagcaata tttattgctc cactgttaat cactttaacc atttcgggca ttggttattt
     4921 gttctttcca gaagttgaaa ataatattta taagaatgag ttttttggtg acagtgatgt
     4981 caaaacgcat caaacattaa atgatgcagt acatcaagtt gaacgacaat atgaaggatt
     5041 ctttgtaagt aaagttagca tacttgatga accatataac aaacgaatta cactgagtga
     5101 tatggcagga aatcagcgtt acgtctttct agatcataac aatcaatttg ttgcagatca
     5161 aaatgcgaaa catacgtatt ctaatgtgat gcgaagtata catagttctt tgtttactga
     5221 aaatactatt attaattatt tagttgagtt aaccgcatgt tggacgatat tcatgatttt
     5281 atctggtact tatttactca ttaagaagca tttaatttct aacaaaagta aggcacttca
     5341 ttggcaaaag tggcacgcaa tgattggaat tatcattgca attccagtat ttgtattagt
     5401 cttaactgga ttgccatggt ctggttttat gggcagtaaa attgccggta tgatggacac
     5461 aaacggtgac cttggtcaag gtgaattagc gattaatcca cctaaatcag atttgaatga
     5521 attaccttgg gctacacgta aaaataaaca gccagcttca tccgaaaaag gttcaagtgg
     5581 tcatcatggt aatgcagcaa tgcctcaaac taaattagat tatcaaatat ctattgataa
     5641 ggtcgttgaa caggcgcaaa aatctggtat taaaaagccg ttttcaatcg tatatccaag
     5701 tgataaaatt ggcaccttta ttgtatctaa tactagtaat tcaggtgtta ctgggctaga
     5761 tgtatctcct tacaaggaac aaacacttta ttttgatcaa tatagcggta aaaagctagg
     5821 tacgattaaa tatgatgact acggtattat tgctaaatgg tttacgtggg gcattccgct
     5881 tcacgaaggt catttattcg gtattgtaaa caaaatcatt aatttatttg tatgtatcgc
     5941 tttattagta gccattggta tgggatttgt ctcttggata aatcgtacaa aaaatactgc
     6001 agtaaaagta ccacatcgcg taaaaaaaac cagcatctat atcactcatc atatgtttaa
     6061 ttgtattagg actattaatg ccattatttg gattatcact tatcctagta tttataattg
     6121 aattaatatt atatattaaa gatcgtcgtg ctaaacaata atgcacttaa agttttgaac
     6181 tgacgaaatt tacaaaatga ttctctaatt acttaaaaca ggttctataa taaatcgaac
     6241 tgatggagct tgggagataa atcaatgttc tagggtctat aatattatat tggcagtagt
     6301 tgactgaatg aaaatgcgct tgcaacaagc ttttttcaac tctagtcaac cttgccgggg
     6361 tgggacaacg aaataaattt tgtaaaaata tcatttctgt cccactccca tctgtccgat
     6421 tttttgaatt tgaagataaa aaagcatcta aaacgtataa gaagattact ttacttcttc
     6481 caaattacgt tatagatgct ttttattaat tcatatgcat gtcttgaatt ttcatcatta
     6541 aaccgtgtgg gacgtcatca tgtttaacaa tctcttgttt ataaaatgtc ttcccatcat
     6601 ctttagaaac taattttaca aagtctcctt ttttcggttt aaaatcattt gatggttcta
     6661 ctttgatatt atgtttgact aatccatctt tttcactgac aactttttct gcatttgtat
     6721 tactattcat ataaccaaaa taagtcgttt tttgactaga aaaaatcatt aaacatacaa
     6781 cggcaatacc aataacaacc aatgctatag ataaacctag acccattttt ttacccattt
     6841 atagactcct ccatttttca aaagtaccta ttatcatgag aacataatta ttactagttt
     6901 tagtcacgca ttagaaacac tttagttttc aatagtttta cgttctgtct ttttacccca
     6961 gtattgataa taagtacatt ctatatatcc attaaataac ttacgtcgct tcgttgcttt
     7021 acgatctact aaatattcaa attctttatt acttgttaaa atgtatgtag ataaaaatgg
     7081 atgttgtttc attagtttac caatataacg gtacatttct tcaacttctt cacgatcacc
     7141 aatacgttca ccatatggag gatttccaat taacgcaacc ggttcttctg tatcaattgt
     7201 taatgtattg acatctttta cactaaattt aataatatca gacaacccca cttcttcagc
     7261 gttacgctta gcaatctcta ccatttcagg atcgatatca gaagcatata cttcgatttc
     7321 tttatcataa tcagccatct tatccgcttc atcacggtaa tcatcataaa tatttgctgg
     7381 catgatgttc cattgctctg atacgaactc tcgattaaaa ccaggtgcga tattttgagc
     7441 aattaaacaa gcttctatag ctattgtacc cgaaccgcaa aatggatcaa ttaaaggtgt
     7501 atcacctttc cagtttgcaa gacggattaa acttgctgcc aacgtttctt taattggtgc
     7561 ttcaccttgt gctaatctat aaccacgtct gttcaaacca gaacctgatg tgtcgatagt
     7621 caataataca ttatctttta aaatggcaac ttcaacgggg tatttcgcac ctgattcatt
     7681 taaccaacct ttttcgttat atgcgcgacg taatcgttca acaatagctt tcttagttat
     7741 cgcctgacaa tctggcacac tatgtagtgt tgatttaacg cttctacctt gaactgggaa
     7801 gttaccttct ttatcaataa tagattccca agggagcgct ttggtttgtt cgaataattc
     7861 gtcaaatgtt gttgcgttaa aacgtccaac aacaattttg attcggtctg ctgtgcgcaa
     7921 ccataaattt gcctttacaa ttgcacttgc gtctccttca aaaaatatac ggccattttc
     7981 aacatttgtt tcatagccta attcttgaat ttccctagca acaacagctt ctaatcccat
     8041 cggacaaact gcaagtaatt gaaacatata tgattctcct tttatacagg tattttattc
     8101 ttagcttgtg ttttttatac atttccaaca aatttaatcg ctgatacatt aacgcatttg
     8161 cttatcattt taaaacaaag cagtgtcatt atatcaagat aaggcgttaa ttttaagtgt
     8221 tcttttcatg aaaaaagctc tccatcatct aggagagcta aactagtagt gatatttcta
     8281 taagccatgt tctgttccat cgtactcatc acgtgcacta gtcacactgg tactcaggtg
     8341 ataaccatct gtctacacca cttcatttcg cgaagtgtgt ctcgtttata cgttgaattc
     8401 cgttaaacaa gtgctcctac caaatttgga ttgctcactc gaggggttta ccgcgttcca
     8461 ccttttatat ttctataaaa gctacgtcac tgtggcactt tcaaattact ctatccatat
     8521 cgaaagactt aggatatttc attgccgtca aattaatgcc ttgatttatt gtttcatcaa
     8581 gcacgaacac tacaatcatc tcagactgtg tgagcatgga ctttcctcta tataatatag
     8641 cgattaccca aaatatcact tttaaaatta taacatagtc attattagta agacagttaa
     8701 acttttgtat ttagtaatta tttaccaaat acagcttttt ctaagtttga aatacgtttt
     8761 aaaatatcta cattatttga agatgtattt gttgttgtat tattcgaaga aaaactttta
     8821 ttgtcctgag gtctagatgt tgctacacgt agtcttaatt cttctaattc ttttttaagt
     8881 ttatgattct cttctgataa ttttacaact tcattattca tatcggccat tttttgataa
     8941 tcagcaataa tgtcatctaa aaatgcatct acttcttctc ttctatagcc acgagccatt
     9001 gttttttcaa aatctttttc ataaatatct tttgctgata atttcaatga aacatctgac
     9061 attttttcca cctcattaga aactttgatc ttcagaccac tgtaagtcat tgatgaatgc
     9121 tgttaattca tcgaacgtca caatatcaca agtatagttt gttttatcca taaaatcaac
     9181 taacatctgc ttgaagaact taggactcgc ctcttgttcc tcatcataaa tgagcaatgt
     9241 ttgatccgaa tgttcaagca taaattgatc tgcttgttta aattgaaaag gaccttgata
     9301 cgacgtatga aaaatactat caacataatc tgcatgctta attatgttgg catacttact
     9361 ttgattatgt tcattccact tttctgtatg tccttggaac ggtgtaatta cagcaaactt
     9421 taacgaatca tatgttcgtt gtaattcaat aacaacttct gcagtccata attctatacc
     9481 catttgccct tgtattaaca cccattctaa tccttcatcc aacagttgtt caattttatg
     9541 ttttataaat tgttttaaat aatgtacttc aggtgcgtca tctttaaaaa tgtttaattc
     9601 gaatgatttg taacctgtta cataaactgt tttaaccata agtaccctct ttagttacgc
     9661 catctaatat attttgtaaa tcatattgaa cacttttaag cttttcaata aataactttc
     9721 gacttgttcg cttaaaatga cattcgacag aaagttgttc aatgttagca atcaatagct
     9781 caaatttcct tgaattcata taaggtactt ctataataaa ttcacgatgt aatttgatct
     9841 cattgagcaa gctatcaata tgttcagtat atggctttac agtttggtaa aaatcatgat
     9901 cttgttgttg tgatttcaca ttttcgaaat tttgttgcat gttgttaact tcataaatta
     9961 gtgattcgac tacatcattc atgaggacgc ctcctacatt ttttaattta tcacaatata
    10021 ctgtattcgt catgttttaa cactctatat aatttgattt aacttatttt caaatgtgtt
    10081 atctgttaaa tcaagtaaat ctaaaaactt cctatataaa tacaaaattt tatcgtgtat
    10141 gttgttatac gatgaaaata cttttaatct aataaaatca tttaaatcaa atac
//
LOCUS       DATTQF010000091         9964 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.091, whole genome shotgun sequence.
ACCESSION   DATTQF010000091 DATTQF010000000
VERSION     DATTQF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.091"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..213
                     /locus_tag="VB241_001867"
     CDS             <1..213
                     /locus_tag="VB241_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEP1245156.1"
                     /translation="GIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKVIDYCPIDQ
                     IKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            489..1088
                     /locus_tag="VB241_001868"
     CDS             489..1088
                     /locus_tag="VB241_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP1245157.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            1208..3283
                     /locus_tag="VB241_001869"
     CDS             1208..3283
                     /locus_tag="VB241_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEP1245158.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADESIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETKGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDDVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            3396..3545
                     /gene="rpmG"
                     /locus_tag="VB241_001870"
     CDS             3396..3545
                     /gene="rpmG"
                     /locus_tag="VB241_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP1245159.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            3754..4293
                     /locus_tag="VB241_001871"
     CDS             3754..4293
                     /locus_tag="VB241_001871"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEP1245160.1"
                     /translation="MTKNEIRKYILHKMKTFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDKKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            4305..5768
                     /locus_tag="VB241_001872"
     CDS             4305..5768
                     /locus_tag="VB241_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP1245161.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSTIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            5749..5952
                     /locus_tag="VB241_001873"
     CDS             5749..5952
                     /locus_tag="VB241_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP1245162.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            5949..6935
                     /locus_tag="VB241_001874"
     CDS             5949..6935
                     /locus_tag="VB241_001874"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP1245163.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            6935..7264
                     /locus_tag="VB241_001875"
     CDS             6935..7264
                     /locus_tag="VB241_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEP1245164.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            7261..7884
                     /locus_tag="VB241_001876"
     CDS             7261..7884
                     /locus_tag="VB241_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1245165.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            7936..8910
                     /locus_tag="VB241_001877"
     CDS             7936..8910
                     /locus_tag="VB241_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEP1245166.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            8882..9952
                     /locus_tag="VB241_001878"
     CDS             8882..9952
                     /locus_tag="VB241_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP1245167.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNKILSLIGYSDTIVLQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
BASE COUNT         3720 a         1303 c         1775 g         3166 t
ORIGIN      
        1 gggattattg aaaatacaga acttgatggt gaaatgaagt ttagaattgc ttgtacaaat
       61 catcatcatc atcattttat ctgtgaaaag tgtggagata caaaggtaat agattattgt
      121 ccaatagatc agataaaatt atcactacct ggtgttaata ttcacaaaca caaacttgaa
      181 gtttatggtg tatgtgagtc ttgccaagat taatataaag aaatgagatt tatgcacatt
      241 tggtcagatg tatgcataaa tctcattttt taaattatag aagataaagg ttcatttcat
      301 actccgttcg tatgatttta tggcaaatct gttaactttt tatatacaaa atgtttaatt
      361 atttttgatt tgagttatat taaatgagta gagaaaataa taagtaatag aatttaagca
      421 aattctttgt tgaaccagtc actgcttgtt attatttact tacagacatt ttaggaggat
      481 gattatttat ggcttttgaa ttaccaaaat taccatacgc atttgatgca ttagaaccac
      541 attttgacaa agaaactatg gaaattcacc atgacagaca tcataacact tatgttacga
      601 aattaaatgc tgcagtagaa ggtacagatt tagaatctaa atctattgaa gaaattgttg
      661 ctaatttaga cagtgtacca gctaacatcc aaactgctgt acgtaataat ggcggtggac
      721 atttaaacca ttcattattc tgggagttac tttcaccaaa ctcagaagaa aaaggtactg
      781 tagtagaaaa aattaaagaa caatggggtt ctttagaaga atttaaaaaa gaatttgctg
      841 acaaagcagc tgcacgcttt ggttcaggtt gggcttggtt agtcgtaaac aatggccagt
      901 tagaaattgt gactacacca aaccaagata atccattaac tgagggtaaa acacctattt
      961 taggtttaga cgtatgggaa cacgcttatt acctaaaata tcaaaacaaa cgccctgact
     1021 acattggcgc attttggaat gtagttaact gggaaaaagt tgacgaatta tataatgcaa
     1081 caaaataaag tatgattact tgttcggtct cttaaatgag accgttattt tttgtcaaaa
     1141 agatagaaat aatttctaaa ttcatatatg atttaaagtg aaagactttg aatagaggta
     1201 ggtagttttg ttaaaaagac taaaagaaaa atcaaatgat gaaatcgttc aaaatacaat
     1261 taacaagaga attaacttta tatttggtgt gattgtattt atttttgcag tactagtact
     1321 acgtttaggt tatttacaaa tcgcacaagg ctcacattat aaacaaatta taaaaaatga
     1381 tgaaaacatt acagtgaatg agtctgtgcc aagaggtcgt attttagaca gaaatgggaa
     1441 agttttagtt gataatgctt ctaaaatggc tattacatat actaggggtc gaaaaacaac
     1501 acaatcggaa atgttggata cggctgaaaa gttatcaaag ctaatcaaga tggatactaa
     1561 gaagattaca gaacgtgata aaaaagattt ctggattcag ttgcatccta aaaaagcaaa
     1621 agcaatgatg acaaaagaac aagctatgtt agcagatgaa agtattaaac aagatcaata
     1681 tgataaacaa ctgttatcga aaatcggaaa atcacaatta gatgaattgt cttctaaaga
     1741 tttacaagtt ttagccattt ttcgagagat gaatgcagga acagttttag atccacaaat
     1801 gataaaaaat gaagatgtca gtgaaaaaga gtatgcagca gtttctcagc aactttccaa
     1861 attaccaggt gttaacacgt ctatggattg ggatagaaaa tacccatatg gcgacacttt
     1921 aagaggtata ttcggagatg tatcgacacc tgctgaaggt attcccaaag aattgacaga
     1981 acattactta tccaaaggat attcacgcaa tgatcgtgtt ggaaaatctt acctagaata
     2041 tcaatatgaa gatgtattgc gtggtaagaa gaaagaaatg aaatacacaa cggacaaatc
     2101 tggtaaagtt acatcttcag aagtgttaaa tcctggtgct cgcggtcaag atttgaaatt
     2161 gacgatcgat atagatcttc aaaaagaagt agaagcatta ttagataaac aaattaagaa
     2221 gcttcgcagc caaggtgcaa aagatatgga taatgctatg atggttgtac aaaatcctaa
     2281 aaatggagac attcttgcgc ttgccggaaa gcagattaat aagagtggta aaatgactga
     2341 ttatgacatt ggtacgttta cttctcaatt tgcagttgga tcttctgtaa aaggtggaac
     2401 attattagct ggttatcaga ataaagctat caaagttgga gaaacaatgg tcgatgaacc
     2461 attacatttc caaggtggtt tgacaaaacg atcatacttt aataaaaacg ggcatgtatc
     2521 tattaatgat aagcaagctt tgatgcattc atcaaacgta tatatgttta aaacagcatt
     2581 aaaattagcg ggagaccctt attattctgg tatggcttta ccatcagaca taagttcacc
     2641 tgcccaaaag ctaagaagag gattgaatca agtaggctta ggtgtgaaaa caggtataga
     2701 tttaccaaat gaaacaaaag gtcaaatcga accattaaca aataatccag gtaattatct
     2761 agatttatca attggtcaat atgataccta tacaccatta caattatcac aatatgtttc
     2821 aactatagcg aatgatggtt atagaataca accacacatt ggattaacga ttcatgaatc
     2881 aactaataaa gatgatgttg gtccactcaa gaagaaaatt aatggcactg tcttgaacaa
     2941 ggttaataat actgaaaaag aaatcaaaca aattcaagaa ggattcaaaa tggcatttaa
     3001 tgataaagat ggtactggat atgttagttt taaagataca gtagtgccta ctgctggtaa
     3061 aactggtacc gctgaagtgt tccaaaacgg agagccaaga gttaactcta cttatatagg
     3121 atacgcgcca attgatgatc caaaattagc gttttcaatt gtatatacaa atcagcctgt
     3181 accaccacca tggttaacag gtggagactt aggtagagat gtaattaact actactttaa
     3241 gcagttaggt aaagacgata aaaataaaga caaagacaaa taaaatttaa cctgacgatt
     3301 atgtagcgca tggttgtaaa attttaactt tgcaaatatt atagatgttg gtataataat
     3361 aaagtcgtat ttagaaatga taaggaggaa taaccatgcg cgtaaacgta actttagctt
     3421 gtacggaatg tggtgacaga aactacatta caactaagaa caaaagaaat aatccagaac
     3481 gtgttgaaat gaagaaattc tgttcacgtg aaaacaaaca aactttacac cgtgaaacaa
     3541 aataatcatt aaaattaaat ttaaattaac aataaaaaag gtgaaatctc aaaataagag
     3601 gtttcacctt tttaattgtg cacacgtatg aaatgataaa agattttcaa ttgaaataat
     3661 aaattttaga attgtcgcat aattcatcat gacaacataa tgacatgtat tgttattaac
     3721 gatataatag aattgaataa agtgggtgat aatgtgacta aaaatgagat tagaaaatat
     3781 attttacata aaatgaagac ttttaataaa gctgaaaagc gaaaagcaga cacatggtta
     3841 agaaatcaat tttttgcaac tgaagaatac aaagaagcaa acgcaattgc gctagttctt
     3901 tcttttaatc atgaagtaga tactttttct attattgaac aagccttaat ggatcataaa
     3961 cgtatttttg tgccgaaaat ggattattta aatcatcaaa tgacttttaa agagatattt
     4021 aatctaaaag atattgatgt agataagaag gggatttact atccaacttc aaaaggtgaa
     4081 acaacgaata acctagattt aattgttgtt cctggtgttg gatttcaaga cgatggatat
     4141 agaattgggt atggtggtgg ctattatgac aggtttttag ctaattatca gacaaagaca
     4201 ataagcttat tatacgattt tcaaataaca tcatttgaac cggaatcatt cgatcaacca
     4261 gtcgataaat tgattatata tcaatcagca tagtggagga ctaaatgaac atagacaaac
     4321 aattttggaa aacaatatat tattggatta ggtacttgaa ttttgatatt gtcagtaggg
     4381 aaaaagatga ccaagaaatt tggttagccc ataaaagaaa aaaacaagta gtcattttta
     4441 aacaacatat caagtctacc caagaaatac gttttgacaa agcgaaagtg cttgaacaca
     4501 aagatgaaat agcaaatttt atttctttcg aaccacaaag ttttgaattt tattatttta
     4561 cagaatcgga attttcagaa gaacaattaa atgaagtttc gacaattaga attaaattca
     4621 atgttataag acacacaaaa gatttgataa agcatatgcc gaatatattt ttggctagac
     4681 ttatttcaga agataatgat aaaaagacat atatgttcta taaacgcaaa gtattaaccg
     4741 ataacttttt agataaatat atgcagaaat tttcaccggc aacatacaca ataatatttg
     4801 taaatgtctt aatatggtta tgtatgattt tatatttaaa taatttttcg gatgtaaaat
     4861 tattagatgt tggcgggtta gtgcatttta atgtcgtaca tggtgagtgg tatcgaattg
     4921 ttacatcgat gtttttacat tttagttttg aacatatact tatgaatatg ctttcattat
     4981 ttatttttgg taaaatagtc gaagcaatta ttggttcatg gaggatgtta actgtatact
     5041 ttattgcagg gctgtttgga aactttgtat cactatcatt taatacgact acaatttcag
     5101 ttggggctag tggtgctata ttcggtctga tcggatcaat ttttgcgatg atgtatgttt
     5161 caaaaacatt taacaaaaaa atgttaggac agttattaat tgcattagtg atattagttg
     5221 gtgtttctct gtttatgtca aatataaata ttgtggcgca tattggaggc ttcattggtg
     5281 gtttattaat aactttaatt ggctattact ataaagtgaa tcgtaatatt ttttggattt
     5341 tactaatcgg tatgcttgtt atatttattg cacttcaaat tagaattttt acaattaaag
     5401 aagataatat ttataataaa ttgatcaaag atgatatgac tagtggtaat tatgataatg
     5461 ctcaaaatat tgtgaagcag acaataaata aaaattatgc cgatgatcaa acatattatt
     5521 taagtggtat gattatggct actatcaatt ctaagtctga aggtatgaca gaatgggagc
     5581 gaggacttag aatgtttcct aaatcaggtt tattaaattt tgagttagcg atagcaaatc
     5641 gttcattaaa tgatgatgaa aaagcattaa aatatgtgcg taaagcatta aatgcagacc
     5701 ctaaaaatac agattatatt aacttagaaa aagagttgac taaatcaaat gagtcgaaaa
     5761 ataaataact tttatgatgt acaacagtta ttgaaaagtt acggatttct aatatatttt
     5821 aaaaatccag aagatatgta cgaaatgatt caacaggaga tttcatcatt gtatcaatat
     5881 gaactgttat ctaaggaaga atatttgaaa tgtacgttga taattaatca gagaaggaat
     5941 gaacagaaat gacaaaaatt attttagcag ctgatgtagg cgggacgact tgtaaattag
     6001 gtattttcac acctgaatta gaacaattac ataaatggtc tattcacact gatacatctg
     6061 atagtacagg ttatacactt ttgaaaggaa tttatgattc gtttgttgaa aaagtaaatg
     6121 aaaataatta taatttttca aatgtacttg gcgtaggtat tggtgttcca ggtcctgttg
     6181 actttgaaaa aggtacagta aatggagcag taaacttata ttggccagaa aaagttaatg
     6241 tacgtgagat ttttgaacaa ttcgttgatt gtccagtgta tgtagataat gatgctaaca
     6301 tagctgcttt aggggagaaa cacaaaggtg ctggtgaagg tgccgatgat gttgttgcca
     6361 tcacacttgg tacaggacta ggtggaggaa ttatttcaaa tggtgaaatc gtacatggtc
     6421 ataatggctc tggcgcagaa ataggtcatt ttagagcaga cttcgatcaa cgatttaaat
     6481 gtaattgtgg tcgttctgga tgtattgaaa cagttgcttc agcgacaggc gttgttaact
     6541 tagtcaactt ctactatccg aagttgacgt ttagatcttc tatattagaa ttgattaaag
     6601 aaaataaggt tacagcaaaa gctgtttttg atgcggctaa agctggtgac caattctgta
     6661 ttttcataac tgaaaaggtt gcaaactata ttggatattt atgtagtatt attagtgtta
     6721 caagtaatcc gaaatatatc gttctaggtg gaggaatgtc tactgcagga cctattttaa
     6781 ttgaaaatat taaaacagaa tatcataatt taacatttgc acctgctcaa tttgaaactg
     6841 aaattgtaca agcgaaatta ggtaatgatg caggtattac aggagcagca ggattaatca
     6901 agacctatgt attagataaa gagggggtaa aataatggct attgttgatg tggttgttat
     6961 tccagttgga acggaaggtc cgagtgttag taaatatata gcagatattc agaaaaaact
     7021 tcaagaatat aaagcgatgg gtaaaattga ttttcaatta acaccaatga atactctaat
     7081 tgaaggtgaa ttaagcgatg tattagaagt tgtgcaagtg atacatgaat taccttttga
     7141 taaaggttta agtagagttt gtacaaatat ccgtattgat gaccgacgag acaaatctag
     7201 aaaaatgaat gataaactaa catcagtaca aaaacattta gaaaatagtg gtgaaaacct
     7261 atgaggattt caagcttaac tttaggctta gttgatacta atacgtattt catcgaaaat
     7321 gacaaagctg ttattctgat tgacccttca ggtgaaagtg aaaaaattat taaaaaatta
     7381 aaccaaataa ataaaccatt aaaagctatt ttattaacac atgcacactt tgatcatatc
     7441 ggagcagtcg atgatatagt tgatcgattc gatgtcccgg tttatatgca tgaagcagag
     7501 tttgattttc taaaagatcc cgttaaaaat ggagcagata aatttaagca atatggatta
     7561 ccaattatta caagtaaggt aactcctgaa aagttaaacg aaggtagcac agaaatagaa
     7621 ggatttaagt ttaatgtgtt acacacacct ggacattcac caggaagttt aacatatgtg
     7681 tttgatgaat tcgcagttgt tggagataca ttatttaata atggaatcgg acgtacagat
     7741 ttatataaag gtgactatga aacgctagtt gattctattc aagataaaat atttgaatta
     7801 gaaggcgatt tacctttatt ccctggacat ggtccatata cgacggttga tgatgaacaa
     7861 ttaaatccat ttttacacgg ttaaattgaa gtaataaaaa cacctcctac gtgattatat
     7921 gtaggaggtg tttttttgaa gattctattt caagaaataa ttaataaagc gatagaaatg
     7981 aaagcgagtg atgtacattt tattccagtt aaaaatgaag taagtattaa atttagaatt
     8041 aatgataact tggagcagta tgaacaaatt gggaatagca tttatcaaaa gttattagtt
     8101 tatatgaagt ttcaagctgg gcttgatgtt tctacacagc aagtcgcaca gagcggtcga
     8161 tatagttacc atttcaataa aatatatttt ttgagaatat caactttacc attgtcactt
     8221 ggccaagaaa gttgtgttat cagaattgta cctcaatttt ttcaacaaca gaaatcaact
     8281 tataaattca atgattttaa acacctcatg aataagaaac aaggattact attgtttagt
     8341 gggccaactg gttcaggaaa gagtacatta atgtatcaaa tggtctcata cgcgaataaa
     8401 gccttgaatt taaatgtaat ttctatagag gatcctgtag agatgcaaat tcctggtatc
     8461 gtgcaaatta atgtgaatga taaagctggc attaactatg taaattcgtt taaagctatt
     8521 ttaagatgtg atcctgatgt tattttaata ggtgaaatca gagataaaga tgttgccaag
     8581 tgtgttatac aggctagttt aagtggtcac cttgttctga ctacattgca tgcaactgat
     8641 tgtaaaggtg ctattttaag gctattagaa atgggcattt ctgtacaaga attgatacag
     8701 gcaactaact taattataaa ccaacgactt gtaactacta ttaagcaaca gcgacaatta
     8761 gtatgtgaaa ttctatctca gcaacaactc cgatatttct tttcccataa tcattcatta
     8821 ccatcatcat ttaagaactt agaagataaa cttgatgata tgacaaaagc aggtgtcatt
     8881 tgtgaaacta caatggataa atacatttaa actacattct aagaagcgac aattaagtaa
     8941 ggcccaacaa atcgatttac tttcaaattt atgtaatttg ttgaaatatg gtttcactct
     9001 gtatcaaagt tttcaatttt taaatcttca aatgacatat aaaaataagc aattaggtac
     9061 caccattcta agtgaaattt caaatggtgc accatgcaat aagatattat cactgatagg
     9121 ttatagcgat actatcgtcc tgcaagtata tttggcagaa agatttggca atatcataga
     9181 cgttctagaa gaaaccgtaa attatatgaa agtgaataga aagtcagaac aacgattgtt
     9241 aaagacactg caatacccct taatactagt ttctattttt attgctatga ttattatatt
     9301 aaacctcaca gtaattccac agtttcaaca attatatact tctatgaata ttcaactatc
     9361 ttcttttcaa aaaacattgt cttttttcat taccagctta cctactataa ttgtagtaat
     9421 gctcataata gtatctatgt tggctattat tatgaaatta atttataaca atttaaatat
     9481 gctcaataag ataaactttg tgatgaaact accgctaata tcaggctatt tccaattatt
     9541 taaaacttat tttgtaacta atgaattagt gttgttttat aaaaatggta ttacacttca
     9601 atcaatagta gacgtttata ttaaccatag tagtgatcca tttagacagt ttctaggtaa
     9661 atacttatta acttattcag aaatgggata tggtttgcct caaattttag aaaaactaaa
     9721 atgctttaag cctcaattaa ttaagtttgt gctacaaggt gaaaagagag ggaagctaga
     9781 agtagaacta aagttatatt cgcaaatatt agtaaaacaa atagaagata aagcgataaa
     9841 acagactcag tttttacagc ctattttatt tttgatttta ggtttattta ttgtcgcaat
     9901 ttatttagta attatgttac cgatgtttca aatgatgcaa agtataaaat aatggaggtt
     9961 aata
//
LOCUS       DATTQF010000092         9948 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.092, whole genome shotgun sequence.
ACCESSION   DATTQF010000092 DATTQF010000000
VERSION     DATTQF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.092"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..578
                     /locus_tag="VB241_001879"
     CDS             <1..578
                     /locus_tag="VB241_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1245168.1"
                     /translation="DPEYGVKIYNQRTELINGIGRFTLTSQELFYLFNLILKYISTVP
                     LALIFLFTTNPSHFAASLNQLGVNYKISYAVSLALRYIPDIQETYFNISQAQQARGYD
                     NSKKAKFTSRVKGIKRIVLPLIFSSIERIDTISTAMELRQFGQYKRRTWYVKKQLKKD
                     DYVVLCLTLILLMLVVTLFFLNNSRYFNPWH"
     gene            625..1122
                     /locus_tag="VB241_001880"
     CDS             625..1122
                     /locus_tag="VB241_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245169.1"
                     /translation="MINTERLNLMIPSSSHLIDLYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWQNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLNFKRDDTMDDIINQGD
                     IVFYK"
     gene            1240..2364
                     /locus_tag="VB241_001881"
     CDS             1240..2364
                     /locus_tag="VB241_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP1245170.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAMHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKVKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLDGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDKAGFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            2668..3486
                     /locus_tag="VB241_001882"
     CDS             2668..3486
                     /locus_tag="VB241_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEP1245171.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSLVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            3504..4118
                     /locus_tag="VB241_001883"
     CDS             3504..4118
                     /locus_tag="VB241_001883"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEP1245172.1"
                     /translation="MLRIAIAKGRLMDSLINYLDAIEFTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGIADIGIVGSDILDERHYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            4111..5361
                     /locus_tag="VB241_001884"
     CDS             4111..5361
                     /locus_tag="VB241_001884"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEP1245173.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQMNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGSQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFDQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            5377..6390
                     /locus_tag="VB241_001885"
     CDS             5377..6390
                     /locus_tag="VB241_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HEP1245174.1"
                     /translation="MIYIDKNESPVTPLDQKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPVLTLNPDFFMYQAYAAQVNREIAFVDA
                     RSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKAINGYFVID
                     EAYLDYGKAYDVEMAPHILRMRTLSKAFGIAGLRLGVLMSTVETIQRIQTIEHPYPLN
                     VFTLNIATYIFRHREETSRFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            6359..6937
                     /gene="hisB"
                     /locus_tag="VB241_001886"
     CDS             6359..6937
                     /gene="hisB"
                     /locus_tag="VB241_001886"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEP1245175.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYISMDETLAR
                     VVVDISGRPYLSFNATLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            6934..7512
                     /gene="hisH"
                     /locus_tag="VB241_001887"
     CDS             6934..7512
                     /gene="hisH"
                     /locus_tag="VB241_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEP1245176.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            7505..8209
                     /locus_tag="VB241_001888"
     CDS             7505..8209
                     /locus_tag="VB241_001888"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEP1245177.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTEWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVKQLSDI
                     PIGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            8206..8964
                     /gene="hisF"
                     /locus_tag="VB241_001889"
     CDS             8206..8964
                     /gene="hisF"
                     /locus_tag="VB241_001889"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEP1245178.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKLTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEAIRQGGIAVR"
     gene            8961..9593
                     /locus_tag="VB241_001890"
     CDS             8961..9593
                     /locus_tag="VB241_001890"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEP1245179.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
BASE COUNT         3560 a         1447 c         1954 g         2987 t
ORIGIN      
        1 ttgatcctga atatggtgtg aagatttata atcagcgtac agagttaatc aatggtattg
       61 gtcgatttac gctaacatca caggaattat tctatctgtt taatctaata ttaaaatata
      121 ttagtacagt tcctttagcg ttaatatttt tattcacaac gaatccgagt cattttgctg
      181 caagtttaaa tcagctaggt gtgaattata aaatcagtta tgcagtatca ctagcattaa
      241 ggtatattcc agatattcaa gaaacatatt ttaatatttc acaagcgcaa caagcaagag
      301 gatatgataa ttcgaaaaag gcgaaattta ctagccgtgt taaaggtatc aaacgcattg
      361 tgttaccttt aatattttct agtatcgaaa gaattgacac tattagtact gctatggagt
      421 taagacaatt cggacagtat aaaaggagaa cctggtacgt caaaaaacaa ttaaaaaaag
      481 atgattatgt tgttttgtgt ttgacgttaa tacttctgat gttagtagtt acattattct
      541 ttttaaataa tagtcgatat ttcaacccgt ggcattagta ttcatataaa tagtctttaa
      601 atagaaatag gagggagaca tttaatgata aatactgaaa gattaaattt aatgattcca
      661 agttcctcgc atttaattga tctttataat atttgtagtc atccacaagc aaatatatac
      721 actcccaaag gtttacataa ttccaaatta gacacacaac ggtggattga aaaatggcaa
      781 aaccattggc aacaatatca atttggttac tttgtattgg tgaaaaaaat agattgtagt
      841 gttattggta tttgtggata tgaatatcga caattaaagc aagaaacagt attaaattta
      901 ttttataaat tacatccaag ttttgaagga caagggtacg catgtgaggc tattacagca
      961 atcacaaatt ttgtgaatta tatcgatcaa gaaacagtaa aagttatcag gacaaataag
     1021 tgtaaccaac gttcaataaa tttagcagaa agacttaatt tcaagcgaga cgatacaatg
     1081 gacgacatta tcaatcaagg agatattgtg ttttataaat aaaatactat gacattatct
     1141 aaaaaataaa attaaaatcc caacagtttc ttaaaaagtt atgagccaca taataaaaat
     1201 gttataattg tgcataataa aacataggga gaatgtttaa tgcataaaaa atatttattg
     1261 ctattttttc tgttattatc agttgcattg ttatatagct gttccaagca aaatgagaaa
     1321 gctcaaaata aatctgttaa tccaaatgag tatgcaatgc ataaatcgaa aatggatgaa
     1381 cattgggaaa catatcacgg ccaagtttat catgtgtttt atcatccatt aattacagac
     1441 ccaaaagttg cgtttacagg agatacacac caagctaagg gcaacaatga ttggatgatt
     1501 actgtaagtg aatttaaaaa atcattgaac gaattatata aaaataatta catcatcgtt
     1561 aatccacatg atgtatacga cttatcatct catcatgtaa aagccaaaaa agtaaaattg
     1621 ccgaaaggta aaaagccgct aatcatctca attgatgata tgaattacta tagttacatg
     1681 agacaccatg gttatgctga ccgcttagta cttgataaaa ataagcatgt tgtatctgaa
     1741 acgacagact caaaaggcca tgctacttat tctgatgaca atgatatcgt gccaatttta
     1801 aataaatttg taaaagagca tccagatttt tctttaaatg gtgaaaaagg tgttgttgct
     1861 ttaaccggtt atgagggtgt attaggatat cgcacgaatg aattaacgag taaagattat
     1921 acaaagaata aaaaggcagc tgaggaagtt gttcgcgcaa tgaaacggga tggatggtca
     1981 tttgcgagtc attcatatgg tcacattaat tttgaaaaaa catctttaga tggtattaaa
     2041 cgtgatacta aaagatggaa agatgaagtt gaaccaattg taggtaaaac agatatgttt
     2101 gtatttccac atggcgcaca ggatagacat acacaagcgt atgattattt ggtcgataaa
     2161 gcaggcttta aatttattgc aggtgtcggt ccaaacaatt ttacagatat atcagcaacg
     2221 aacgtttacc aagatagggt tgcgattgat ggtctaaact tatttgaatt taaatataaa
     2281 ctcaaaccgt tttttaatcc tgaaaatgta tatagtaaac aagatagaca attctttaaa
     2341 ggtaatcggg attatgaaga atagttaaat ttaataaaaa gatagaaaga aaataacttt
     2401 acataataag gcaaggacaa tatgtgcaag aagtacaact tcaatataaa aatcttggtt
     2461 aagcattttt ataggcacaa atgtcttgcc tttttctttt gaaaaaattt aaaaccaata
     2521 caccgaattt tctgaaaaac gttgtaatgt attgctaaaa atgataagtt attaagcata
     2581 ttacacttta gtatggtaga ggagtaaagt gaacaacaaa aataccaggt gattttaaat
     2641 acagtattga aagtaaaggg tggtcgagtg aataattcag aacaattaat tgcgttaaaa
     2701 gaatcggaaa cagcattttt aaaatatttt aataaagcag attatgagtt agttgatttt
     2761 agtttggttg aaaagttgga ctggaaacaa ttgaaccatg aggatttgca gcagatgggt
     2821 gagcgaaatt tttggcaaca tgagcatcaa atttatgcac ttcgaaatga ttttacagat
     2881 caattattgc gttattactc aatgtatcca actgcagcaa caaaagtggc atacacaggt
     2941 ttaatcattc ggaataatga agcggctgta caagttggtt tggaaaacta tgcaccatca
     3001 ttagcgaatg tacaacagag cttaaagcta tttattcagt ttattcaaca gcagttgaga
     3061 gacaacgtgc attttgttgt attgggacat tatcaattgc ttgatgcgtt actcgataaa
     3121 tcgttacaaa caccagacat actatcaatg attgaggaac gtaatttatc gggattggtt
     3181 acatatttgt cgactgaaca tccaatcgtg caaatcttga aagagaatac gcaacaacaa
     3241 ttaaatgtat tggaacacta tattccaaat gaccatccag cactcgttga attaaaaata
     3301 tgggaacgtt ggttacatac acaaggttac aaagacatcc atttagatat tactgcgcag
     3361 ccacctagat cctattacac aggtttattt attcaatgtc attttgctga aaatgaatct
     3421 cgagttttaa caggtggtta ttacaaagga agcatcgaag ggtttggatt aggattaaca
     3481 ctttaagtaa gggagtatgc acaatgttaa gaatcgccat agcaaaaggg cgtctaatgg
     3541 atagtttaat taactattta gatgcaattg aatttacgac attatcagaa acattaaaaa
     3601 atagagaacg ccaattatta ttaagtgtag ataatataga atgcatttta gtaaaaggga
     3661 gtgacgtgcc aatctatgtg gaacaaggaa ttgcagatat tggtatcgtt ggtagcgaca
     3721 ttttagatga gcgtcattac aatgttaata atttgttgaa tatgcctttt ggagcatgtc
     3781 attttgcggt tgcagcgaaa cctgaaacga ccaattatcg taaaatcgca acgagttatg
     3841 ttcatactgc tgaaacatat tttaaatcaa aaggcattga tgtcgaattg attaaattga
     3901 atggttctgt tgaattagct tgtgtcgtcg atatggtaga cggaattgtc gacatcgttc
     3961 aaacaggtac tacgctaaaa gcgaacggac tggttgaaaa gcaacatatt agtgatatca
     4021 atgcaaggtt aataactaat aaagcagctt attttaaaaa atcacaatta atagagcaat
     4081 ttattcgctc tttggaggtg tctattgcca atgcttaatg cacaacaatt tttaaatcaa
     4141 ttttcattag aagcaccatt agatgagtca ttgtatccaa ttattcgcga tatttgtcag
     4201 gaagttaaag ttcatggaga taaggctttg aaaatgtata atctaacatt cgatcatacg
     4261 aaaacagatc atttagaaat tagtcatgaa caaattaaag cagcatttga cacattagat
     4321 gaaaaaacaa aacaagcatt acaacaaagt tatgaaagaa ttaaagcata tcaagaaagt
     4381 attaagcaga tgaatcaaca gttagaagaa tcagtggagt gctatgaaat ataccatcca
     4441 ctagaaagtg tgggtattta tgtgcctggt ggcaaagcaa gttatccatc aacggttcta
     4501 atgacagcga ctttagcaca agtagcgggt gtagaaaata ttgttgttgt gacaccacct
     4561 caacctaacg gagtatccca agaggtatta gctgcatgtt atattacgca agttaatcaa
     4621 gtgtttcaag ttggtggttc tcaaagtatt gctgcattga cttatggaac agaaacgata
     4681 cctaaagttg ataagattgt aggtccaggt aaccaatttg ttgcatatgc caaaaaatat
     4741 ttatttggac aggtaggtat tgatcaaatt gcaggaccaa cagaaatagc actgattatt
     4801 gatgacaccg cagatttaga tgccatcgta tatgatgtat ttgcgcaagc agaacatgat
     4861 gaattagcac gtacatatgt cattagtgaa gatgcgcaag tccttaaaga tttagaatca
     4921 cgtattgcta aagcattgcc taatgtggac agatacgaca ttgtttctaa aagtatcgct
     4981 aatcaacact accttatcca tgctagtaat tttgatgaag catgccacgt tatgaataca
     5041 atcgcgcctg aacatgcgtc gattcaaaca gtaaatcctc aaccatatat tgaaaaagtg
     5101 aaatatgtgg gcgcattgtt tattggacat tattcgccag aggtcatagg tgattatgtc
     5161 gctggaccta gtcatgtatt acctacaaat agaacagcta gatttaccaa tgggttatcg
     5221 gtcaatgatt tcttaacacg gaacacggtc attcatttat caaaagatac atttgatcaa
     5281 attgctgatt cagcacaaca tattgctcat gttgaagcat tatacaatca ccaacagtct
     5341 attttaatac gtcagtctta ggggagtgta aatgaaatga tttatattga taaaaatgaa
     5401 agtccagtta cgccgttgga tcaaaaaaca atgacgtcta ttattagtgc aacgccatat
     5461 aatttatatc ctgatgcagc atatgaacaa ttcaaggaag cttatgctaa gttttacgga
     5521 ttatcgcctg aacaaattat tgcaggaaat ggctctgatg aattgattca aaagttaatg
     5581 ctgatcatgc cagaaggtcc ggtattaacg ctaaatccag atttttttat gtatcaagca
     5641 tatgcggcac aagtaaatcg tgaaattgca tttgtagatg caagatcaga tttaacgttt
     5701 gatttggaaa ccattttaac gaaaatcgat gaagtacaac catcattttt tattatgagt
     5761 aatccacata acccttcagg caagcaattt gatacggcat ttttaacagc tattgcagat
     5821 aagatgaaag caataaatgg atactttgtc attgatgaag catatttaga ttatggcaag
     5881 gcatatgatg ttgaaatggc accacacatt ttaagaatgc gcacattatc aaaggcgttt
     5941 ggaattgccg gcttaaggtt gggtgtatta atgagtactg ttgaaacgat acagcgtatt
     6001 caaacaatag aacatccata tccattaaat gtatttacgc taaatattgc gacttatatt
     6061 tttagacata gagaagagac aagtcgattt ttaacaatgc aacgacagtt agctgagcag
     6121 ttaaaacaaa tatttgatac acatgttgca gataaaatgt cagtattccc atcaaatgct
     6181 aattttgtac ttactaaagg ttcagcggcg caacaattag gacaatacgt atatgaacaa
     6241 ggatttaaac ctcgttttta tgatgagccg gtgatgaagg gctatgtaag atattcaatt
     6301 gcaacagcat cacagttaaa gcaattagaa gaaattgtta aagaatggag tgcaaaatat
     6361 gatttatcaa aaacaacgaa acacagctga aacacaacta aatatttcaa tatcagatga
     6421 ccagtcacca tcgcatatta atacaggtgt gggcttttta aatcatatgt taaccttgtt
     6481 tacgtttcat agtggactat cgttaaacat cgaggcacaa ggtgatatcg atgtagatga
     6541 tcaccatgta actgaagata tcggcattgt tattggccaa ttgttacttg aaatgattaa
     6601 agataaaaag catttcgttc gttatggaac gatgtacatt tcaatggatg aaacattagc
     6661 acgtgtcgtt gtggatataa gtgggcgccc atacttatct ttcaatgcaa cgttaagtaa
     6721 agaaaaagtt ggtacgtttg atacggagtt agtagaagaa ttttttagag cagtcgtaat
     6781 caatgcaaga ttaacaacgc atattgattt aattcgtgga ggtaatacac accatgaaat
     6841 tgaagctata ttcaaagcat tttcccgtgc attaggcata gcgctaactg caactgatga
     6901 tcagcgtgtg ccgtcatcga aaggtgtgat tgaatgattg tcatcgttga ttatggatta
     6961 gggaatatta gtaatgtaaa acgtgctatt gaacatttgg ggtatgaggt ggttgtctca
     7021 aataagcaaa atataatcga tcaagcagaa acaatcatat taccgggtgt cggtcatttt
     7081 aaagatgcga tgtcagagat aaagcgatta aatctcgatg caatattggc taagaatact
     7141 gataagaaaa tgatcggtat ttgtttaggc atgcaattaa tgtatgagca tagtgatgaa
     7201 ggcgatgcat ctggattagg gtttatccca ggaaatattt cgcgtatcca aacagaatac
     7261 ccagtgccgc acttaggttg gaataattta gtgagtaagc atcctatgtt aaatcaagat
     7321 gtttacttcg tacattctta ccaagcgccg atgtcagaaa atgtcattgc atatgcgcag
     7381 tatgggactg atattccagc aattgttcaa tttaacaatt atattggtat tcaattccat
     7441 cctgaaaaaa gcggtacata tgggttacaa attttgcgtc aggcaataca agggggattt
     7501 ataaatgatt gaattatggc cagcgattga tttgattggg tcaacaagtg tgaggttaac
     7561 agagggtaaa tatgatagtg aagaaaaaat gtcgcgttcg gctgaagaaa gtattgctta
     7621 ctatagtcaa tttgaatgtg tgaatcgtat tcatatcgtc gacttgatag gtgctaaggc
     7681 acagcatgcc cgagagtttg attatattaa gtcattgagg agattaacaa ccaaagatat
     7741 tgaagtaggt ggtggcattc gcacgaagtc acaaatcatg gactactttg ccgcaggaat
     7801 taattattgc atagttggaa cgaaaggtat tcaagataca gaatggctta aagagatggc
     7861 acatacattt ccaggtcgca tttatttatc tgttgatgct tatggagaag atattaaagt
     7921 gaacggatgg gaagaggaca cagagttgaa tttatttagt tttgtgaaac agttatcgga
     7981 tatacctatt ggaggcatta tttatactga tatcgctaaa gatggcaaaa tgtccggacc
     8041 taactttgaa ttaactggtc aattagtaaa ggcaacgacg attcctgtca tcgcgtctgg
     8101 tggtattaga catcagcaag atattcaacg attagcgtcg ctaaatgttc acgctgctat
     8161 tataggaaag gctgcacatc aagcatcatt ttgggagggg ctaaaatgat taaaaaacgt
     8221 atcattccat gtttagatgt caaagatggt cgtgtcgtta aagggattca atttaaagga
     8281 ttaagggata ttgggaatcc tgttgattta gcaatgtatt acaatgaagc gggtgctgat
     8341 gagttagtat ttttagacat ctctaagacg gaagagggtc atagcttaat gctagaagtg
     8401 attgaacaga cagcgtcacg cttgtttatc cctcttactg tagggggtgg gattcaaagc
     8461 cttgatgata ttacccaatt gttaaatcat ggtgcagata aagtatcatt aaattcaagt
     8521 gctttaaaaa atccacagct aattaaacaa gcgagtgata aattcggtag acaatgcatc
     8581 tgcatagcaa ttgatagcta ttatgatcct gaaagaaaag cacattattg ttgtacgcat
     8641 ggcggtaaaa aattgacaaa tattaaagta tatgactggg tacagcaagt agaacagtta
     8701 ggtgcaggtg agctcctcgt tacaagtatg gggcatgatg gtatgaaaca aggctttgat
     8761 attgagcatc ttgcaaaaat aaaatccctt gtaaatattc caatcattgc ttctggtggt
     8821 ggtggcaatg cacaacactt tgtagaatta tttgatcaga cggatgtttc tgcaggttta
     8881 gctgcaagta tattacatga ccgagaaacg acggttcaat ctattaaaga agcgatacgg
     8941 caagggggta tagcagtaag atgaccaatt acaaaattga ttttagcaaa ggtttagtgc
     9001 cagcaatttt acaagataat caaacaaaac aagtattgat gttaggttat atgaatcaag
     9061 aagcttttga taaaacgata gaagatggtg tggtatgttt ctattcgcgg tcgaaacaac
     9121 gcctatggac aaaaggtgaa acatctggtc atacgcaacg tgttaaagat attcatgtag
     9181 attgcgataa tgacactatt ttaattgatg tcataccaaa tggaccaaca tgtcatacag
     9241 gcagtcaaag ttgtttcaac acagaagttc cattttcagt acaaacatta gcgcagacag
     9301 ttcaagatag tgcccaatcc aataatgaaa agtcatatac gaaatattta ttaacagaag
     9361 gtatagaaaa gattacaaaa aaatacggtg aagaagcttt tgaagtcgta atcgaagcaa
     9421 ttaaaggtga caaaaaagca tttgtaagtg aagtagcaga tgaactttat catttatttg
     9481 tcttgatgca tgcgctaggc gttgattttt cagaaattga ggcagaatta gcgcgtagac
     9541 atcataagcg caataacttt aaaggcgaac gacaaaatat cgaacagtgg taaagcaagt
     9601 atggactgag atatagagaa aaggatcatg gcttatacac ttacaaatat tgtgggaaac
     9661 gtgacatttt caagtttaaa atacgacacc aacatatttt aactatgaat gctgtgatgg
     9721 tactaaagtt gcgaactcgt tatagataac tagtggataa tcacgatacg aaaccaaaga
     9781 taatttgaaa agtgaattga gcaactcagg aatagatgtc actgttaaag atgttgaaaa
     9841 gtatatgaat cgatataatg aagttatgaa gggaaaaatg gcgaaaaagc taaaaagtta
     9901 tgtttgtcgt tactacctat taatatcata gttgtcttta tattcttt
//
LOCUS       DATTQF010000093         9934 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.093, whole genome shotgun sequence.
ACCESSION   DATTQF010000093 DATTQF010000000
VERSION     DATTQF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.093"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(7..825)
                     /locus_tag="VB241_001891"
     CDS             complement(7..825)
                     /locus_tag="VB241_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEP1245180.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(836..1459)
                     /locus_tag="VB241_001892"
     CDS             complement(836..1459)
                     /locus_tag="VB241_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245181.1"
                     /translation="MELSQRQERIIEIVKTQGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            1794..3185
                     /locus_tag="VB241_001893"
     CDS             1794..3185
                     /locus_tag="VB241_001893"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEP1245182.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(3336..4082)
                     /gene="recO"
                     /locus_tag="VB241_001894"
     CDS             complement(3336..4082)
                     /gene="recO"
                     /locus_tag="VB241_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP1245183.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNKWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDVAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKYDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(4110..5009)
                     /gene="era"
                     /locus_tag="VB241_001895"
     CDS             complement(4110..5009)
                     /gene="era"
                     /locus_tag="VB241_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP1245184.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(5010..5414)
                     /gene="cdd"
                     /locus_tag="VB241_001896"
     CDS             complement(5010..5414)
                     /gene="cdd"
                     /locus_tag="VB241_001896"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP1245185.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSHFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(5425..5769)
                     /locus_tag="VB241_001897"
     CDS             complement(5425..5769)
                     /locus_tag="VB241_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP1245186.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(5772..6239)
                     /gene="ybeY"
                     /locus_tag="VB241_001898"
     CDS             complement(5772..6239)
                     /gene="ybeY"
                     /locus_tag="VB241_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP1245187.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPDIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(6240..7187)
                     /locus_tag="VB241_001899"
     CDS             complement(6240..7187)
                     /locus_tag="VB241_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP1245188.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGTVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(7490..8188)
                     /locus_tag="VB241_001900"
     CDS             complement(7490..8188)
                     /locus_tag="VB241_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEP1245189.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(8205..9194)
                     /gene="floA"
                     /locus_tag="VB241_001901"
     CDS             complement(8205..9194)
                     /gene="floA"
                     /locus_tag="VB241_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEP1245190.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(9212..9895)
                     /locus_tag="VB241_001902"
     CDS             complement(9212..9895)
                     /locus_tag="VB241_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEP1245191.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         3099 a         1872 c         1410 g         3553 t
ORIGIN      
        1 atgaaatcat ttcgatttat tttgttcaat ataatggatt atatcgtttg ctgtttcttc
       61 gattgctttt tgagaaacat caatgacagg acatccaatt tcacttacga tttcttcaaa
      121 gtaattcaat tcttcttgaa ttcgtgcttc tgttgcatat cgagctgtgt cacctagtcc
      181 taattgtttt agtcgctctt ttctaatgcg atttaatttt tcttcactta ttttaagtgc
      241 gatacatttc tttggatcaa tatcatataa gccatctggc ggtgtcactt ctggtacaat
      301 cggtacattc ataactttgt aacttttatg cgctaaatac tgggataatg gtgtctttga
      361 agttctcgaa ataccaagta aaacaatatc agctttaggt aatcctttag gatctttacc
      421 atcatcatat ttaactgcaa actctatcgc atcaattttc ttgaaatatg catcatctaa
      481 tctatgaacg atacctggct cattataagg tttttcttca actgatgctg ataataaatc
      541 cattaatggc cccataatat cgacggactt caattggaat tctgctactt tctcactcat
      601 atattgcttc atttcaggtt taataagtgt ataaacaatg atagcatttg tatctttagc
      661 aacttgaatc acttcatcaa catcttcaaa agattcaata tatggatatc ttaataattc
      721 atttttacat tgcttaggat taaattgtga aatacctgcc ctagcaacta actctgccgt
      781 ttcacctata gaatctgaag ctacgataat tttaattttt tccattactt ctcacctatt
      841 ctttatataa tgctactaat aacttagcta ttgttgtttt ggaaattctt ccaattactt
      901 catacttttg attatctttt tttcttacaa ttggaatcga atcaatttct ttttcaatca
      961 ttctatctgc tgcgtatatg actaattcgc tttcctctaa ataagtgaca ttaggcatac
     1021 gtgtcatatt aacactgata ggtactgtat gaatatctgc tccaatcatt gaagctctta
     1081 ataaatcttt tcttgaacac acaccaacaa aatcgttatc ttcattaata ataaataatg
     1141 tacttacatc ttctaaaaaa attgtacaaa tagcgtcata aactgttgta ttctctctta
     1201 gcacaacagg ttgagacata tagtccttaa cctcaaattg tcgaagtttt tcattaaaaa
     1261 atttaccttt tgatttacct gaataataat atccaactcg gggacgcgct tctaaaaaac
     1321 ctgacattgt taatatcgct aaatctggtc taagcgttgc tcttgttaaa ttcaacttat
     1381 ctgctatttg ttcaccagta atgggtcctt gagttttaac aatttcgatg attcgttctt
     1441 gtctttgact gagttctata ggtcttcacc ccttttttat tcacttatta ttataccgat
     1501 atttctttca tgtcacaaac acattaattt tacttgcctt taaataatct atcaattaga
     1561 ataaacgtac agcgagttaa ggatagtgta agcttaacaa taagattggc gcaacgaatc
     1621 attttaaaat aaaagcgagt gactacacta atttgggtgg aaccgcgggt taaatacgta
     1681 acttattttg tattatatac aatttaaaat attaactcgt cccatggaag taaagcatat
     1741 ttactatgct gttattttcc atggggcgtt ttcatgtatg aggagaggta attatggcaa
     1801 aagatatgga tacaattgtt tcattagcaa aacacagagg ttttgtgttc cctggtagtg
     1861 atatttacgg tggtttatca aacacatggg attatggtcc tttaggtgtt gaattaaaga
     1921 ataatgttaa aaaagcttgg tggcaaaaat tcattacaca atcaccgttt aacgttggta
     1981 tcgatgctgc aatcttaatg aatccaaaag tatgggaagc ttcaggacac ttaaacaact
     2041 tcaacgaccc aatgattgat aataaagata gtaaaattcg atatcgcgct gataaattaa
     2101 ttgaagatta tatgcaagat gttaaaggtg atgaaaactt cattgccgat ggtttaagtt
     2161 ttgaacaaat gaaaaaaatt attgacgatg aaggtattgt ttgtcctgta agtaaaactg
     2221 ctaactggac tgaaattcgc caattcaatt taatgtttaa aacattccaa ggtgtaactg
     2281 aagattctac aaatgaaatt ttcttacgtc ctgaaacagc acaaggtatt tttgtaaact
     2341 ataaaaacgt gcaacgttca atgcgtaaaa aattaccatt tggtatcggt caaattggta
     2401 aatcattccg taatgaaatc actccaggta acttcatttt cagaacaaga gaatttgaac
     2461 aaatggaact tgaattcttc tgtaaacctg gtgaagaaat cgaatggcaa aattattgga
     2521 aaacttttgc aagtgactgg ttaacaagct taaatatgag tagtgaaaat atgcgtttac
     2581 gtgatcatga tgaagatgaa ttatctcatt actcaaatgc aacaactgat attgaatata
     2641 aattcccatt tggttggggt gagttatggg gtatcgcaag tcgtacagac ttcgacttac
     2701 gtaaacatgc tgaacactct ggtgaagatt tcagatacca cgatccagaa acgaacgaaa
     2761 aatatattcc atattgtatc gagccatcac ttggtgcaga tcgtgtaaca ttagctttct
     2821 tatgtgatgc atatgatgaa gaaggcgttg aaggtagtaa agatgcacgt acagttttac
     2881 acttccatcc tgcattagca ccatataaag cagcgatttt acctttaagc aagaaattat
     2941 ctggcgaagc gattaagatt tttgagcaat taagttctaa attctcaatc gatttcgatg
     3001 aatcacaatc tatcggtaaa agatatcgtc gtcaagatga aattggtaca ccttattgtg
     3061 taacattcga ctttgattca ttagaagata atcaagttac agtacgtgac agagattcaa
     3121 tggaacaagt tcgtatgcca atctcagagt tagaagcttt cttaactgaa aaaacaaaat
     3181 tctaattaaa aagactctcc accatatgtg gtggctatat aatttataaa aagagatact
     3241 ggttagtaaa attgccagta tctcttttct attcgaaaaa ggtattcaaa taaacacatc
     3301 tatgtggcta cttgaatacc ttttattatt ttatgttatt gttccaatct ttttaattgg
     3361 ttgattagtt tctgactttt aaaaaacata cctgcatatt cacgatataa cattaaaatg
     3421 atatctgaca tttcatcaat aatttcttga tggatattca atgaattcat tttatctatt
     3481 ggtaattttt gtaatacatc taataaatat agtgttttat tcgataatat aactgcatgt
     3541 acatctttag aagcctcttg ccttgaaata gcaccgtcat acttaaaact ataacctatt
     3601 aaatctgctt gtgtgtcatt accactcaca gcacagcgat taaatgatgc ggtaaaacca
     3661 aatcgtttca tacactttaa cataacaacg actgacatta actgtgcaga tgtacctgat
     3721 tctatttttt caagaacaaa ttgtaataat tgatagttat atggtgcaac gtccccttca
     3781 tccattgaac gctcaatagt ttcagctgcc agagacgcat aactgcttac gtaaaggtcc
     3841 atttgtaatt tataatgttg actaataaca tctacagaat ttaacgttcc catacctcgc
     3901 cacttattgt aaataaacaa accataaaca aacaattgcg tttgcgcttg caaacccgtc
     3961 ttaacttttt tagcacgcct tgccataagt ggtacttttg caccatgctc atttaaaatc
     4021 gtgataattt tatcagattc accataatca actgctttga tgataatccc tttttggcgc
     4081 attaacaatt atcttcacca cttttaagat taatcttggt cttcaacata accaatttgg
     4141 cgaataaagt taactttgtt tcgccagtct ctttgaactt tgacccataa ttccaagtaa
     4201 actttagagc ctagaagcat ttctatatca cgtctcgcac gttttcctac ttcttttaac
     4261 tttttaccgc cttttccaat gacaattcct ttttgcgaat ctctttcaac atatatagtt
     4321 gcttcgatat gaacacgatc ttcgctttct ttaaccatac ggtccacatt aacaccaatc
     4381 gcatgaggga tttcttcact tgtaagatga aggatttttt cacgaatgat ttcacccact
     4441 acaaattgtt caggatggtc tgaaatttga tcatctggat aatattttgg tccttcgggt
     4501 aaatacgtct ttaatacatc aataaaatga tctacattta gcccttctaa tgctgaaata
     4561 ggtacaatct ctgtaaagtc catataactt tgatattctt caatctttgg cattaattca
     4621 tctggatgca ctaaatctat tttatttaat actaaaaata ctggtgtctt aacatttttc
     4681 aacatttcta taatatattc atcgcctcgt ccaatttcct cattggcatt aaccataaac
     4741 atgattgcat ctatctcaga taatgtattt ttagcgactt tcatcatata gtcacctaat
     4801 ttgtgtttag gtttatgaat acctggcgta tcaatgaata taatttgcgc atcatctctt
     4861 gtcattacac cttgaatttt atttctagtt gtttgagctt tatcggacat gattgctatt
     4921 ttatggccaa tcactctatt aacaaatgtt gacttcccta catttggtct acctataatt
     4981 gaaacaaatc ctgatttatg ttctgtcatt tattctaaat cctttcctga aaatccaaat
     5041 ggtagtaact ctgcgactgt catcataacc atatctcctt tatgatttgt catatacaca
     5101 ggcatatcat catcacataa ttccttcaaa acttgacgac atgcaccaca aggtgatgac
     5161 ggtttatctg catctacggt tacagttatt gattcaaaat cacctggtct gtatccttga
     5221 gaaattgccg ataccaaact agctcgttca gcacatatcg ataatggata agaagcattt
     5281 tctacattgg taccataaaa agttctaccg tctttcgttt ttaaataagc ccctacttta
     5341 aaatgactgt atggcgaata tgattcttgt tgtgcttttc taacttcttg aaaataatga
     5401 ggttgataac tcatatatgc ctccctaaaa taacgctata aaatgtggta aaaatactat
     5461 taaacctata ataaatgcta atattgaaac tataagtaca ctaaaagccg ctatatcttt
     5521 agcgtattta gctaaatcat gatattcaac ggtcactaaa tctacaacat attcaatagc
     5581 agtgtttaaa gcttcaacag tgagaactaa tgcaatagca atgagtataa atatccactc
     5641 aatccgatta atatttagta cgagaccaaa gacaatagca acaatcattg caaacacatg
     5701 taaaagaaat ttatagtctt tttgaattaa gattttcagc ccatcaagtg catatttaaa
     5761 ccttttcata attagtctcg tgttaatcca tatgcgttta atattgtatc ttgtcgacca
     5821 aacatttcct tttcatccgc ttcagtcata tgatcataac ctaatagatg caaaaatcca
     5881 tgtaatgcta aaaatcctaa ttctcgttca aaagaatgtc cgtaattgtt tgcttgttct
     5941 tgcgctacat ccgtacagat aattatatcc cctaaaacac gtggtatatc aagaccacta
     6001 aaatcaatat ctggctcatc ctcttctaaa gcaaatgaga ttacatctgt aactttatct
     6061 ttatctctat atgttcgatt aatttcttgt atttcttgtt tatctacaaa tgtaacagaa
     6121 agctcagcat cgtcttctat atgctcttct tttttagcaa attctaataa atcttcaatt
     6181 tgtttatacc aagcatcttt aactaagcct gtgtgatcgc taaaatctat cgtaaacatt
     6241 taattctctc cttcataatg ttcaatgatt ttacttacca atggatgtct tactacatcg
     6301 ctctgatcta atttcaatat acttatacct ttaacgttgt gtaacctact gaccgcttcc
     6361 ttaagtccac ttttaacacc tttaggtaaa tcgatttgag tttggtcacc agtaactacc
     6421 atttttgagc caaaacctag tcttgttaaa aacattttca tttgcgcatg tgtcgtattc
     6481 tgcgcctcat caagaattac aaatgcatct tctaatgttc gtccgcgcat atatgcaagt
     6541 ggcgctattt cgataatgcc tctttcaata aatcgctctg tttgttcacg cccaagaaca
     6601 gtatatagac catcatataa aggtcttaaa tatggatcta ccttttcttt caaatctcct
     6661 ggtaaaaatc caagtgactc tcctgcttca acagcaggtc ttgttaatac aatacgttta
     6721 acagtacctt tacggagttg ctttgctgca taaactacag ctaagaatgt cttacctgta
     6781 ccagcaggac ctataccaaa tactaaatca ttatttttca tggcattaac atatatacgt
     6841 tgccccatcg ttttcgcacg aatcgtctta ccaaatgcat ctttagttat ctcttcatca
     6901 tataaatcta acagatgttg aattgtgtta ttatgcgcca ttttaatagc tgcttcaaca
     6961 tctttaattg taatattatt acctaaatca ataaccttca gcaaattgat taatactgat
     7021 tccgcttttt ctacgttttc tatttttgta cctttaacgg caacttcttg tcctcttgca
     7081 tggatgacaa catcgaaact ctcttcaatt gcttttaaat gttcatcatt atttccaatt
     7141 aaagcttgag attggttcat atcgtctatt tgtataattc caggcataca cgcgctcctt
     7201 ttcatatatc atttatattc attctatgat gctttaatat ctacttaatt atatcatgca
     7261 attgattgat ttaaacatct ttgcattgac ttactgattt ttctttaatt taaaataatc
     7321 atttatactt ttcaccttgt cataaaaaca gattcaattt tagactaatc aattttttgt
     7381 tgtaatttaa acaactcata tttctcgaaa gattctcttt gattttcaat ttttcttaac
     7441 gtaaaaaaac aagcaactac cattacgata gtgcttgttt tcaaaaattt tataattgtt
     7501 ttggtttagc taaaatttct gaccatatca taccattgat tacttcatct ttatcaaatt
     7561 ggaacgctga tttagtcaaa tctttttcga cattttgaga attaagcaac tgctttaatt
     7621 tcaaacgttt tgtacgttca gataagtatt tatcctcaat aatatctcta gcacgttttt
     7681 ccattttagc aatttgcttt tctttttctc tatcaatgtc agtacgaatc gttttaatat
     7741 catctctaag tgatttttct aattgacgtc taatttcgtc agaattatct tgtctagaag
     7801 gtctatgttt ctgctccacc ggttctgctt taggcactgt aattggtttc tctgtcatcg
     7861 gttttgtttc ttgttgtttt ttaggtgcca taggttctac aacaggtttc gatttaggct
     7921 cttcctttgg aagttcatcg aataaaggtg gtaacgtatc atcgtatttt cgtttcgatg
     7981 atttcttttc ttcttcattt aattcttcac ttatttcttt aaacgttcgc tcaatttctt
     8041 caaaaaagcc accttttttt ggttcattat cggtagatgt tttttgaggt ggcttttgat
     8101 tttgtctatc tttatgacta ttttcgcgca tagtagtaat gatagaaatg atcactgata
     8161 tgacaaaaat tagaataccg acactcattt aatcacctct cgacttaatg ttcaggtgac
     8221 tcatcatcac tttgatcagt tcgtttatta attgcatttc tcatgcctgt atcagcttcg
     8281 atatttttca aattataata atctttaaca ctgatattac ctgaacgtaa tgcttcagcc
     8341 atagctaatg gtacttcaga ttcggcttca actactttag catgcatttc ttgtacacgc
     8401 gctttcattt cttgctcagt tgctacagcc atagctctac gttcttcagc ttttgcttgt
     8461 gcaatatttt tgtctgctaa tgcttgttca gtttgtaagt ctgcaccaat atttttacta
     8521 atatcaacgt cagcaatatc aattgataaa atttcaaatg cagtacctga atctaaacct
     8581 ttgcttaata ctgttttaga aatattatct gggttttcaa gtacttctgt atgatgctta
     8641 ctagaaccaa ttgttgaaac gataccttca ccaacacgtg cgatgattgt ttcttcacca
     8701 gcaccaccaa caagtcgagc aatattagct ctaactgtga tacgagcttt ggctttcact
     8761 tcaataccat tcattgctac acctgcgata aatggagttt caatgacttt aggattaaca
     8821 gacatttgaa ccgcttctaa tacgtcacgt cctgctaggt caatagcagc tgcacgttcg
     8881 aaaggaaggt caatgtcagc acgttgtgca gcaatattag cgtcaacaac tctgtcaaca
     8941 tttcctcctg ctagataatg cgattctaat tggtttgttg ttaatgctag tcctgcttta
     9001 tgcgctttaa ttaatggcgc tataactttt cttggagata cacgacgtaa acgcatacca
     9061 accaatgtac ctataccaac atgaacgcca gctgctaacg ctgaaatcca taaaccaatc
     9121 ggtacaaatg agaataaaat aagtaatgca actactataa taactgctat tacgataaaa
     9181 cttaaactaa acatggtatc gctcctttta attaatctac ttccctcaca actactcttg
     9241 ttccttcaac ttcaaggatt tttaccgttt tattgcgtaa aataaagttg ccatctgaaa
     9301 cagcatcaat acgttcattt tcacaaaaaa taatccctgc aggtcgaaga tctgtaactg
     9361 tttgagcagt ctttcctacg aggtgcgagc ggttatcatg agaattgtaa cctgactcag
     9421 aattagttga atcttttaag ataactttat ccaaaaacgg aatctttctg ttgaaaatct
     9481 tcactaatat cacccattct acaatcgtta aaatcaaggc aacgataaca ttcgcaagca
     9541 taaatagcaa attatcaccg agcgttgtta tgcttatagt tatcagtatc atgccaataa
     9601 taccaattac tgcaccaact acaaataatt caattacaac taatataacg ccaattgaga
     9661 atattaagat agaatgcata ttgacatttc cttggattag aaatcccaaa aataaaataa
     9721 gtaatgataa tgtggcgata ataccagctg cattgatttt tttagagtaa agttgatata
     9781 caaatcctaa gaatgtcaaa caggttaata ttaacgtaaa aataggttga acaattatat
     9841 tactaacttg ttcaacccaa gtatctccag ccgttgattc caagatagtt gtcatttgta
     9901 aaaaattatt ataactcaca atttcacctc gccc
//
LOCUS       DATTQF010000094         9912 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.094, whole genome shotgun sequence.
ACCESSION   DATTQF010000094 DATTQF010000000
VERSION     DATTQF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.094"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            291..364
                     /locus_tag="VB241_001903"
     tRNA            291..364
                     /locus_tag="VB241_001903"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:325..327,aa:Arg,seq:tct)
     gene            complement(826..1023)
                     /locus_tag="VB241_001904"
     CDS             complement(826..1023)
                     /locus_tag="VB241_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1245192.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANGIFSDENYEE"
     gene            1270..2217
                     /locus_tag="VB241_001905"
     CDS             1270..2217
                     /locus_tag="VB241_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP1245193.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAICGIFAFLIWI
                     HLLCTMLKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINNLITPIW
                     ILCLVGIMMHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     ILLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPVIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            complement(2533..2760)
                     /locus_tag="VB241_001906"
     CDS             complement(2533..2760)
                     /locus_tag="VB241_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245194.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVTYANMVFDWNLRWYLLGNI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(3067..4623)
                     /locus_tag="VB241_001907"
     CDS             complement(3067..4623)
                     /locus_tag="VB241_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP1245195.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            complement(4794..5726)
                     /gene="arcC"
                     /locus_tag="VB241_001908"
     CDS             complement(4794..5726)
                     /gene="arcC"
                     /locus_tag="VB241_001908"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP1245196.1"
                     /translation="MAKIVVALGGNALGKSPQEQLKLVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFTFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            complement(5749..6750)
                     /gene="argF"
                     /locus_tag="VB241_001909"
     CDS             complement(5749..6750)
                     /gene="argF"
                     /locus_tag="VB241_001909"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP1245197.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            7180..7905
                     /locus_tag="VB241_001910"
     CDS             7180..7905
                     /locus_tag="VB241_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HEP1245198.1"
                     /translation="MKKNITNKIVLSTALLLLGTTSTQPPKAPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSFSGKWLWQNPNGTIHATLQTWVWYTHIQVFGPESWGNINQL
                     RDKYVDIFGTKDEDTIEGYWTYDETFTGGVTPAATSSDKPYKLFLKYKDKQQTMIGGL
                     EFYQGNKPVITLKELDFRVRQTLIKNKKLYNGEFNKGQIRITGGGNHYTIDLSKKLKL
                     TDTNRYVKNPRHAEIEVILEKSH"
     gene            8015..8740
                     /locus_tag="VB241_001911"
     CDS             8015..8740
                     /locus_tag="VB241_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HEP1245199.1"
                     /translation="MKNNLTKKIILSIALTMIGTATSQSPHSPISMSSEAKAYNISES
                     ETNVNELTKYYTQRHLTFSNKWLWQKDNGTIHATLLQLSWFSHIQVFGPESWGNINQL
                     RNKYVDIFALKDYETWRTYMLAQETFTGGVTPVAKPSDKHYKLNVTYKDKAGTFIGEY
                     QFYTGNKPVLTLKEVDFRIRQTLIKNKKLYNGDYNKGHIKITGGSNNYTIDLSKRLKS
                     TDANRYVKNPQTAHIEILLEKSS"
     gene            8849..9565
                     /locus_tag="VB241_001912"
     CDS             8849..9565
                     /locus_tag="VB241_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HEP1245200.1"
                     /translation="MRKCIMKKLILMTTLLLLGTTATQTSNSPLNVSTDAKAYHIGQD
                     ETNINELIKYYTQLPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNVNQL
                     RDRYVDVFGLKDEDTSQLWWVYRDTFTGGVTPAASPSDKPYSLFVQYKDKLQTIIGAH
                     KMYQGNKPILTLKEIDFRARETLIKNKILYHENRNKGKLKITGGGNDFTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
BASE COUNT         3366 a         1769 c         1404 g         3373 t
ORIGIN      
        1 tcgctcaacg ttttgttgtt gttattttaa aataaatttg atgcaattag tttgtttatc
       61 cgcacaacat cttataatgt acttaactgt actttaaaga gaaaagatat acagttaggc
      121 attcaaaact gtatttaaca caattaagtt gcctgaattc gtatttaagt cttattgaac
      181 ctttttagat aaatagctct ataatagtga aaaatataaa cattttttat ttacaaggta
      241 ttgctaattt aagttcattt agatataata attcttgtgt tgttaaacgt gtcctggtag
      301 ctcagctgga tagagcaatg gccttctaag ccatcggtcg ggggttcgaa tccctcccag
      361 gacgtttata gtatttttat acgcattacc aaacaaaaga gttccgtgat tacggggctc
      421 tttttgtttt gaatttcagt aatatagtat gatgcgtcac caaaacgtcc ccgcacaagc
      481 ccagaaaata cagtgataaa aacaagcatg catattcgtt atagaatttt ttgacacaca
      541 attgacacgc gtctgacact tgtttacaca tttttaatta agtaattttg cgctcaaatt
      601 tcctctatac tgtacctgaa ctacaccaaa actacaccaa gatttttaac actcaccatt
      661 tgcatgcgta gagattttta ttattatatt attcctatag attttgatac tattcaaaat
      721 tttagggact tttcagggac ccgaaatcct ataattataa ttatatacat ctgaaaaata
      781 accacgtcca tcgagacgtg gttttctaat tatttaactc actaattatt cttcataatt
      841 ttcatcacta aatataccat tggcaaattt ataaagtagt tcagcgtttt tcaatgacat
      901 attgtctaat gatctttcat tttttctcat tctgtgtatt gtgctttgtg gcactcctgt
      961 ttgttttgat atatgtaaac tgctcaaatc actgtctaat agtttttgaa tttgatttct
     1021 cattttgtac actcccttgt aaatcttcaa tcatcattta aattaaataa atacttttcc
     1081 cacattccat atttaggtag caaagcatac ctcaaatatc attaattttg agatttaaat
     1141 gtcaattatg tgtctatcaa tccaatatac acactctaaa aacgtaatag tacactctct
     1201 tcttattaat agtccatagc aatcatgata taattagtga agaaaatcac ataagaaagg
     1261 ttgtaaatca tgagacttca aaaagcacct ctagtaacgt caggactagt cttaggatta
     1321 ttaggcctgg gtaatctatt aaaagactta tctcttactt taaacgctat ttgcggaatc
     1381 tttgctttct tgatttggat tcacctttta tgtactatgc tcaaatattt taataatgtg
     1441 aaagaacaat taaacagccc tctagtttca tctgtgttca caacattttt catgtctggc
     1501 tttttaggta ctacttattt aaatacattt tttagtaata taacatttat caataattta
     1561 ataacgccta tttggatttt atgccttgtg ggaattatga tgcatatgat tattttttca
     1621 attaaatatt taaaagattt ttcacttgag aatgtttatc cttcatggac tgtacttttt
     1681 attggtattg ctatcgcagg attgacggca cccgtcagcg gatatttttt cataggtcaa
     1741 ttaacagtaa tatatggctt tgtagctact tgtattgtct tacctatagt tttcaagcga
     1801 ttaaaagcat ttccattgca gacgtcaatc aaaccgaaca catcgacaat ttgtgcacca
     1861 ttttctttag tcgctgcagc atatgttata gcctttccta aagcgaatgc ttttatcgta
     1921 attatacttt tactattagc tcaaatattt tatttttaca tcattataca attgcctaaa
     1981 ttactaaaag aacctttttc gccagtattt tcagctttca cattcccttt agtaatctct
     2041 gcaactgctt taaagaacag tttgcctgtg cttatgtttc cagtcatttg gaagggcctt
     2101 ttgtttatcg aagtgttatt agccactgta atagtactta gagtctttat aggatatctt
     2161 cacttctttt taaaaaagga aaaccaagat aaatttcttc gtaatgcgtc tcagtaacac
     2221 tattaccaag aatttacacg catatttaat aactaaaaaa ggggcaggta atcttgcctc
     2281 tttttagtgt atatcaatgc ctctttgatt taatatcatc ttatttatga acaacaatga
     2341 taccttctga ctaaagctgt agtaactata ctagaacgtc tagttcataa acacgtttca
     2401 ctctattctt tatttaaata atatataagc agcaaacgcc aaggctaata taccaataaa
     2461 ataattaata aagattttta ttttagtttt tgtaatagtt gacaagtttt atcacctctt
     2521 aacatcgata aattaaacgc cgttattttt cctctgccta tcttttatca tttcagaagg
     2581 tactccgaaa ataaacatca gaataaataa tataattggc aaatgtggta tgtttcctag
     2641 caaataccat cttaaattcc aatcaaatac catatttgca tacgtcacaa aatatttact
     2701 aaataataat actacgcata ttccagttag tgtatctaaa acctttgtca aatgtgtcat
     2761 ttatagccat cctttattta tgctattttc acaaccaaat gtatcatgtg ttacaaaaac
     2821 tgtttacagc aaatatatat ttaccacgat aaatataata taccatagat ctaacattac
     2881 attaaaaaca tattttaaaa atatcattat taaatagaac attgtatcaa ttacttacta
     2941 atgttatcat taccgccatt attttctaaa tcaaaataat aatgcacctc gcttagttta
     3001 gtatttctac aaacctacga gatgcatata acccaattag tattttttat aatatagcat
     3061 ttcaaattat gaataaatta gtacttgcgt aattagtact ccgccaccga aaatcaatac
     3121 aaaagcaact accggccata caaatcggaa ccaatgtgaa tatcgcatgt ttaacatttg
     3181 tagtgtggcc ataacaagtc cagtcggcgc taagaataac atagcatatt gaccaaattg
     3241 atatgtcgta acgatgacga accttggtat acctactgta tcagctagtg gtgcaaagat
     3301 aggcattgat aataccgcta atccagaaga tgatggcacg ataaaaccta aacagaagaa
     3361 aataaatagt aatacgatga taaataatgg tccactcata tgttgaacta aagatgatga
     3421 aaagtgtaag attgtatctg aaatcatacc ttcattcaac actaaattaa tacctcgagc
     3481 taatccaata attaaagata cacctactaa acttgatgca ccattgacaa atgcatctac
     3541 agtccctttt tctcccaatc cagatttacc tgtcccagca ataaacatta ttataattgt
     3601 aaatattaaa aatgctgaag ccataactgg gaaccaccaa ccttgcgtca taactcccca
     3661 taccataatt ggaaatggta gtacaaataa tgtaaggatt atcttcttac gcaaagtaaa
     3721 atgggcacta tcgtcatctt ttaacacaga ccattgctgt tcaaaagcat ctttgtcttc
     3781 ataagaatat gacgctttag gatctttttt aattttttta cagtaccaat ataaataact
     3841 aataacaaaa atcgcaccga caatacaagc acctattctc caatacaaac catccgtgaa
     3901 agttgtacca gctgcattag aggcaattac aaccgagaac gggttaatag ttgaaaaggt
     3961 actaccgaca gagctggcaa ggaatatggc accaacagaa acgatagaat cgtatcctaa
     4021 cgctataaat atagggacta aaatcggata gaatgcgaca gcttcttctt caataccaca
     4081 taacgtcccg ccgataatca ttagtattga tacaaacaca attagcataa attcatgccc
     4141 tttagttttc tttgttaaag ctagcaatcc cgattcaaaa gaaccactgg cttgaactac
     4201 gccaataagt ccccctaata caagaatgaa taccatgata tcaaccgctt ctatcgtacc
     4261 ttcaaccatg ctacttgtta tttgttctgg tccagctgga tgttgcttta atctttcata
     4321 agtattcgga attgataccg gcttattaat tgcacctgat ttaaattgtt caatcttaat
     4381 tttaaccccc attttgtcta gttcctgttg cgtacctgga acctttttca cttggttatg
     4441 agggttaact atctttagtt cttgggatga aggttcgtaa gaaagtttag aatatgcacc
     4501 agcaggaata acccatgttg ctataactgc aacaaccgtt aaaatgaata aaatcataaa
     4561 tgcacctggc atttttaatt taaatcgttt tttcttttca ctttcattaa ttgtattttc
     4621 cacggtttca tctccttcga catttaacct agcatttcta ccttaaagat tttataaata
     4681 taaattaaga aagtgcaccc cgcatcaaaa tagaggcatt attttcaggg gtgcacataa
     4741 ataataaaaa tcatgcattt gacatatagt aattgaaaag cgtttcaatt caattacttt
     4801 ttaatcacag tacctacttt accctctaag gcagcatcta attcatttaa tgatgttata
     4861 agcacacttc cttttggatt gttttcaata aatgatatgg ctgcttcaat ttttggtaac
     4921 atacttcctt ttgcaaattg attttcgtct atatatcgtt ttaattcatc aacatttgtt
     4981 gttttcaaag gctgttggtt ttcagtgtta aaattaatat atacataatc aattgcagtt
     5041 aaaataatca attgatcgca ttgaatatta gcacccaaca acgcacttgt tttatctttg
     5101 tctataactg catcaatacc tttaaaacca tcatgttgct ctctaattac tggtatacct
     5161 ccaccaccag cagcaataac gagtgtatca tttttaataa gtgttttaat actctctaat
     5221 tcaataatag agatgggttg tggtgaagga acaacacgtc tataccctct tccagcatct
     5281 tcaacaaatg taaatccttt ttctttttga atttgttcag cttcttcttt gttgtaaaat
     5341 aacccaattg gttttgaagg attgttaaat gccggatcat tttcgtcaac ttcaacttgt
     5401 gtcactagtg ttaccacttg tttatccatt ccaatagaat gcaattcgtt ttgtaagctt
     5461 tcttgtaatt gatagccgat gtaagcttga ctcattgcgc cacattcagc aaatggaaat
     5521 gccggacctt ggttgtgttc tgcagcatag ttaagtccca aattaatgct tccaacctgt
     5581 ggcccattac catgactaat aacaatctca tgtccttttg ttattaatcc tactaatgat
     5641 ttcgcagtat ttttaacaag cttgagttgt tcttgaggtg attttcctaa agcattccca
     5701 cctaatgcta ctactatttt cgccatcata ttcacttcct tatatcattt aaaattcacc
     5761 caatgtagca accatgactg ctttgattgt atgcattctg ttctcagctt cttggaatac
     5821 aactgaagct ttactttcga atacttcatc tgtaacttcc atttctcgaa taccatattt
     5881 ttcaaaaatt tgttgtccaa ttttggtatc agcattgtgg aaagatggta agcaatgctc
     5941 aaaaataaca tttggattac cagttttatc catcatttct ttatttactt gataaggttt
     6001 caataattca agtcgttctt tccatacttc atcaggttca cccattgata cccaaacgtc
     6061 agtgtaaatt acatccgaac cctttacacc ttggtcaata tcatctgtga ttaatatgtt
     6121 gccaccattt tcagcggcaa tatttttaca gcgatttaat aattcatctg ttggatttaa
     6181 ttcttttgga caaactaaat ggaagttcat acccataatg gcagcacctt gcattaatgc
     6241 atttgcaacg ttattacgtc catctccaac atatgtaaag ttaatatctg cataatcttt
     6301 tttcaaaact tcttttgctg ttaagaaatc agcaagaact tgagtaggat gatcttcatc
     6361 agttaaacca ttccacactg gtacgcctga atattcagct aaagtttcta ctgttctttg
     6421 tgaaaaacca cggtattcaa tgccatcata cattccacca agcacacgtg cagtatcttt
     6481 agttgtttct tttttaccca tttgtgatcc agttgggcct aaataagtta cattagcacc
     6541 ttgatcatgc gctgcaactt caaatgcaca tcgcgttctt gtagaatctt tttcaaataa
     6601 cagtgcaata tttttatttt ttaacatagg cttttcagtg ccaatatatt tagcacgttt
     6661 taaatcctcg gagagtgtta ataagaattc tacctcttgt cgtgaaaagt ctaataaagt
     6721 taaaaaactt ctgtttcgta aatttttcat taataacaac tccttttatt ttagttacac
     6781 ctaaattgta agcgcataca aaataaacac aacctactat taaaatttgt aatattttat
     6841 caataattaa atgaacattt tattaatatt aaatttaagt agtaggaaat aattaaaata
     6901 agtactacat ttaaagtata actatttttc aagtagttag aaaattcaat aaataactaa
     6961 tttaacgcaa ttaatatcat aattagcatc agttttaatt tattagcact cttttcattt
     7021 tataattggc aatgttccaa tgcttaaaaa tgaaaaattt tacttaaatc acacgttcaa
     7081 ctgtttatgt ttatttaaca tagtgttagc ttttatttaa ttccgaatcg gtgcaatacc
     7141 ttatatacta tgggtaattc acgcaaagga gattatcata tgaaaaagaa catcacgaac
     7201 aaaattgttt tatcaacagc attgttactt ttaggaacta catcaacaca accgcctaaa
     7261 gcaccaatca gtttttcatc tgaagcaaaa gcatacaata taagtgaaaa cgaaacgaat
     7321 atcaatgaat taatcaaata ttacacgcag ccacaccttt cattttctgg aaaatggcta
     7381 tggcaaaacc ctaacggtac catacatgca acattacaaa cgtgggtttg gtacacgcat
     7441 attcaagtat ttggtcctga aagttggggc aacattaatc aattaagaga taaatatgta
     7501 gatatttttg gtaccaagga tgaggatact atagaaggct attggactta tgatgaaacc
     7561 tttactggag gtgtaacacc agcagctact tcatctgata agccttataa attattttta
     7621 aaatataagg ataaacaaca aactatgatt ggtggacttg agttttatca aggtaataaa
     7681 ccagtaataa ctttaaaaga actagatttc cgcgtacgtc aaactttaat taagaacaaa
     7741 aagctatata atggagaatt taataaaggt caaattagga ttacaggtgg tggtaatcat
     7801 tatacgattg atttgagtaa aaagttaaaa ctaactgaca caaaccgtta tgttaaaaat
     7861 cctcgacatg cagaaattga agtcattctt gaaaaatctc attagtatca ttcattgagt
     7921 gaatacaaat agactgaatg atttaattga tttctctctt tttataaact tattattcct
     7981 cgcatataac attcctcgta aaggagattt tctaatgaaa aacaacctca caaaaaaaat
     8041 tattttatca atagcattga ctatgatagg cacagctaca agtcaatccc ctcattcacc
     8101 gattagtatg tcatctgaag caaaagcata caatataagt gaaagcgaaa cgaatgtaaa
     8161 tgaattgact aaatattaca cacaacgcca tttaacattt tcaaataaat ggctatggca
     8221 aaaagataat ggtacaatcc atgcaacatt gttacagtta tcatggttta gtcatattca
     8281 agtattcggc cctgaaagtt ggggtaatat taatcaatta agaaataaat atgtagatat
     8341 ttttgcttta aaagattatg aaacctggcg cacttatatg ttagcccaag aaacatttac
     8401 tggcggtgtt acaccagtag caaaacctag cgataaacat tataaattga atgtaacata
     8461 taaagataaa gcaggaacat ttattggcga atatcaattt tatacaggta ataaaccagt
     8521 tttaacttta aaggaagttg atttccgaat ccgccaaaca ctaattaaaa acaaaaaatt
     8581 atataatggt gattataata aaggccacat taagattaca ggtggcagca acaactacac
     8641 aatagattta agcaaaagat taaaatcaac tgatgcaaat agatatgtta aaaaccctca
     8701 aactgcacac atcgaaatcc tccttgaaaa atctagctaa catcgaaatc gaagtaaaca
     8761 gaaataagca caatgtttac attgacgccg ttcattttta aatttgttaa ttctcgtatt
     8821 gatgattacg cagaaaggag aagttcgaat gagaaagtgc attatgaaaa agttaatttt
     8881 aatgacaaca ttgttattat taggaacaac agctacacaa acctctaatt cgccattaaa
     8941 tgtttctaca gacgctaaag cttatcatat tggccaagac gaaacgaaca tcaatgagtt
     9001 aattaaatat tacacacaac tgcctctcac attttcaaat agatggttat accaatatga
     9061 tgacggcaac atttatgttg aatttaagcg ttattcttgg tcagcgcata tacgattatg
     9121 gggtgctgaa agttggggca acgttaatca attaagagac cgctatgtag acgtgtttgg
     9181 tttaaaagac gaagacacta gccagttatg gtgggtatat cgtgatacat ttacaggtgg
     9241 tgtaacacct gctgcatcac cttctgataa accatatagt ctttttgtcc aatacaaaga
     9301 taaactacaa acaattattg gtgcacataa gatgtaccaa ggtaataaac caatattaac
     9361 attgaaagaa attgattttc gagcacgtga aacactaatt aaaaataaaa tattatatca
     9421 tgaaaatcgc aataaaggta aacttaaaat caccggtggc ggcaatgact ttacaattga
     9481 tttgagtaaa agattacatt ctgatcttgc aaatgtttat gttaaaaatc ctcaaaaaat
     9541 aacagtcgaa gtcctcattg attaaataag gtcgtatgat ttatatcgta aatactaagg
     9601 ataaacaata tttgagtaat actacaaaat gtctcaatag tacttacaca atcattaatt
     9661 tagtacacag aatattaaat gcgatttgga gtctaataga aatgccaagt cattctctgg
     9721 gtttgggccc gccccaactt gcattgcttg tagaaattgg taacccaatt tctctatgtt
     9781 ggggccccac cccaacttac attgtctgta gaatttctat tcgaaattct ttttgttggg
     9841 gcccctgtct ctaatttaaa gtcttgttat aatcgcattt ttgtttggtc aatgtctgtc
     9901 tatataatat tg
//
LOCUS       DATTQF010000095         9378 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.095, whole genome shotgun sequence.
ACCESSION   DATTQF010000095 DATTQF010000000
VERSION     DATTQF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.095"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            118..669
                     /locus_tag="VB241_001913"
     CDS             118..669
                     /locus_tag="VB241_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245201.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDIVLDAIEKS
                     CVDATYRTFYQHVLVYEIENKVAGCIISYSGENELKYEKAWEQLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(721..1659)
                     /locus_tag="VB241_001914"
     CDS             complement(721..1659)
                     /locus_tag="VB241_001914"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEP1245202.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISTLFAGI"
     gene            1841..3202
                     /locus_tag="VB241_001915"
     CDS             1841..3202
                     /locus_tag="VB241_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HEP1245203.1"
                     /translation="MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRECFTVEALNDL
                     TDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            3189..4862
                     /gene="menD"
                     /locus_tag="VB241_001916"
     CDS             3189..4862
                     /gene="menD"
                     /locus_tag="VB241_001916"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEP1245204.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQRIEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRVTLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNNVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            4849..5652
                     /gene="menH"
                     /locus_tag="VB241_001917"
     CDS             4849..5652
                     /gene="menH"
                     /locus_tag="VB241_001917"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEP1245205.1"
                     /translation="MTHYNYYEANVETNQVLVFLHGFLSDSRTYHNHIDKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDEARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLIEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            5645..6466
                     /gene="menB"
                     /locus_tag="VB241_001918"
     CDS             5645..6466
                     /gene="menB"
                     /locus_tag="VB241_001918"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEP1245206.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(6681..6887)
                     /gene="sspC"
                     /locus_tag="VB241_001919"
                     /pseudo
     CDS             complement(6681..6887)
                     /gene="sspC"
                     /locus_tag="VB241_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
     gene            complement(6925..8106)
                     /gene="sspB"
                     /locus_tag="VB241_001920"
     CDS             complement(6925..8106)
                     /gene="sspB"
                     /locus_tag="VB241_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HEP1245207.1"
                     /translation="MNSSCKTRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLANNIKEKESAKTVSPQLKQELKTTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYNQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVANAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(8188..9261)
                     /gene="sspA"
                     /locus_tag="VB241_001921"
     CDS             complement(8188..9261)
                     /gene="sspA"
                     /locus_tag="VB241_001921"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HEP1245208.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQT
                     DKQQTPKIQKGGNLKPLEQRERANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNQFNGAVFINENVRNFLKQ
                     NIEDINFANDDHPNNPDNPDNPNNPDNPNNPDNPNNPDNPDNPNNPDNPNNPDNPNNP
                     DQPNNPNNPDNGDNNNSDNPDAA"
BASE COUNT         3188 a         1360 c         1741 g         3089 t
ORIGIN      
        1 ccaatctttc taactttaga attttcaaac tttctaaaat tataagtata tctttttaaa
       61 ataagctaga atttctatat aataaatgtt aataacgtaa aagggaatga tgacatagtg
      121 atacgtcaag cacgtccaga ggaccgattt gatattgcga agttagttta tatggtttgg
      181 gatgatatgg aattagaatt ggtaaagcat ctacctaaag acatagtatt agatgcaatt
      241 gaaaaaagct gtgttgatgc aacatataga actttttatc agcatgtttt agtttatgaa
      301 atagaaaata aagtagcagg ttgtattatt agctatagcg gtgaaaatga attgaaatat
      361 gaaaaagcat gggaacaact tgacttgcca gaagaaataa aacaatatgg cacgccatta
      421 cccgtaaaag aagctaaaga cgatgagtat tatatagaaa caattgcgac atttgaagca
      481 tatagaggta gaggtatcgc gacaaagtta ttaacgtcat tacttgaatc aaatacacat
      541 gttaaatgga gtttgaattg cgatattaat aacgaagcag cattaaagtt atataaaaaa
      601 gtaggcttta tatctgatgg acagattgaa ttatataagc acatgtatca tcatttaatt
      661 gttaaataaa aaaactcgac agttcgatgt aagtcgattg ccgagtagaa tcatcttata
      721 ttaaatgcct gcaaataatg tactaatata aatacctagt gcatataata aaccgaaaaa
      781 tgtatttgtt ttaccagcag cagccattgc tggcatcatt gtaggcggtg tatcattctt
      841 cttgaaacgt ctgataactt taacaggcat tgggaatgat aacaacgcaa gtaagtaaaa
      901 taatgagcca ccaggtttaa taatgatcgt aagtacaata aatgcataag cgataaagta
      961 catgattgcc ataaatgtta aagaagcatt tttacctaat agaatgggta aagttttacg
     1021 accacttgct ttatctttga cacggtcgcg aatattgttg gccatattaa ttaaaccgat
     1081 agtgattact ataggtacac ttaaccaaat tacatagctt tgaatattgc cagtttgaat
     1141 aaagaatgca ataacgataa taaacatacc cataaatacg cctgagaata attcaccgaa
     1201 aggcgtccat gaaataggga aagggccacc tgtatatagg taaccaacag ccatacatac
     1261 taatccaact ggtaataacc aaaatgaaga gttagcagct aaaaacaaac ctaatatcgc
     1321 tgctaagatg taaaatgcaa tggctaatcg tagcacaagc tctgggctca taccgttgcg
     1381 tacaattgca ccaccaatgc ctacagattc atgatcatca aggccttttt tataatcata
     1441 gtattcgtta aacatattag ttgctgcttg aataagtaag catgctagta acatggcaat
     1501 gaataggctg attttaatat gatcttcgct accaagaaaa tatattttag atgctgctgt
     1561 accaactaaa acgggtacta cggaagcagt taatgtatga ggacgcatta aatgccaata
     1621 tttcttaact gtagaatatt gctgatattg attactcatt gaatacaaaa cctctttaaa
     1681 tcatattttt tataacaaag atattttaat agaaagtagc ataaaggtca attgacgtga
     1741 aaaattgcaa gataagttta agcaattctt gtaacacaat tatccaaaaa tgatacaata
     1801 taataatgag tgaaaaaaga cgaagaaaga agtgaaatgg atggctacgg gcgtattaga
     1861 ggacgatatt gtcaaagaga tatatgacag ctcaaaggaa tgggtttcag ttgaagtgaa
     1921 attatcacag tcactagacc cgagcacatt atttcatctc actgacaatg aggcaggaga
     1981 tcgcttttat atgcgtttga atgataatcg aacgtcatat tttggctaca aagcaattca
     2041 attattcaaa aataattcta aaaataaaca atctattttt aaagactggg aaaaattaaa
     2101 acataacatc acatttatac atccgcaatc tgagaaacat catcttcgag ttgttggagg
     2161 gttccaattt tcaagtcata aatcagatga tgaatggcga gagtttggac taaatcattt
     2221 tgtattacct gaagttttaa tttcaacaga taataatggg acatttttaa cttatacagt
     2281 taaaagggaa tgttttactg ttgaggcatt gaacgattta acggatttgt tcaacaatat
     2341 atcggatata gatgtggacg agcaaattgg ggaaattact agaaatgaag atatttataa
     2401 agatgactgg cgtcaacttg tagtagaagc aatagaatct attaataatg aagaaaaaat
     2461 tgtactagca cgtagacggt taataaagtt cgataaagat atcagtattc catatattct
     2521 aaagcaagca tattctaaag aaaaaaacag ttatatattc ttgttagaat cacaagattc
     2581 tatattcttt tcacagacac ctgaacaatt aataaaggtc aataataaaa tactatcgac
     2641 taaagctgta gcaggtacaa ttaaacgttc acaagatgag gacgaagata caaaaaatgt
     2701 tgaagcattt ttaaaagata ataaaaactt aatcgaacat cgatttgttg ttgacagtat
     2761 tttacatgat attaaacctt atatcactga attacattat gataagacgc ctaaaattct
     2821 aaaaaatgat catttatatc acttgtacac tgaaataaag gcgccactga aggatgattc
     2881 gtatattagt ttaattgata atttacatcc aacacctgct ttaggtggct atccaaaaga
     2941 atttgcgatg gattttattg aacagaaaga atttggtaca cgaggattat atggtgcgcc
     3001 ggttggctat atagatatat atgatgattg tgaatttatt gttgcaattc gttcaatgct
     3061 tattaagaaa gcacaagcaa ctttatttgc tgggtgtggc attgttaaag attctgatcc
     3121 agatagtgaa ttggcagaaa cgaaccttaa gttcacacct atgatgaatg cattaggagt
     3181 cgatatgaat gggaaatcat aaagcagctt taacgaagca agtttttaca tttgcatctg
     3241 agttatatgc gtacggcgta agggaagtag ttatcagtcc gggatcacgc tcaacgccac
     3301 ttgcacttgc atttgaagca catccaaata ttaaaacatg gatacacccc gatgagcgaa
     3361 gtgcagcgtt ttttgcagtg gggttaatta aaggcagtga aagacctgtc gctatattat
     3421 gtacgtcagg tacagcagca gcgaattata cgccagcaat tgctgaaagc caaattagta
     3481 gaattccatt aatcgtttta acaagtgacc gtccgcatga attaagaagt gtaggcgcac
     3541 cacaagcgat taatcaagta aatatgttta ataattatgt aagttatgag ttcgatatgc
     3601 cgattgcgga tgatagtaaa gagaccattg atgcaattta ttatcaaatg caaattgcta
     3661 gtcaatattt atatggacca cataaagggc cgattcattt taacttgcca tttagagatc
     3721 cgttaacacc tgatttgaat gcaacagaat tgttaacttc tgagatgaag attttaccgc
     3781 actatcaaaa aagtatagat gcatcggcat taagacacat tttaaataag aaaaaaggtt
     3841 taattattgt aggagatatg cagcatcaag aagttgatca aatactgacg tattcaacga
     3901 tatatgattt gcctatttta gctgatcctt taagtcattt aagaaaattt gatcatccga
     3961 atgttatctg tacatatgat ttgctgttta gaagcggctt agacttaaaa gtggatttcg
     4021 taattcgtgt tgggaaacca gtgatttcta aaaaattgaa tcaatggtta aagaaaactg
     4081 atgcatttca aatattagtg caaaacaatg ataagattga tgtctttcca atagcaccag
     4141 atatttcata tgagatttct gcgaatgatt tctttaggtc attaatggaa gacacgacag
     4201 ttaatcgagt aagttggtta gaaaaatggc aacgcataga gaaaaaaggg cgtaaagaaa
     4261 ttaaatgtta tttggaacaa gctacagatg agagtgcatt cgttggtgaa ttgattaaga
     4321 aaacatctga aaaagatgca ttatttatta gtaatagtat gccaatcaga gatgtagata
     4381 acttgttatt gaataaaaat atagatgtct atgcgaatcg tggtgcaaat ggtattgatg
     4441 gtatcgtatc aactgcactt ggtatggctg tccataaacg agttacatta ttgataggtg
     4501 atttgtcatt ttatcatgat atgaatggac tattaatgtc aaaattaaat aatattcaga
     4561 tgaatattgt attattgaac aacgatggtg gcggtatttt ttcatattta ccacaaaaag
     4621 aaagtgcaac cgactatttt gaacggttgt ttggcacacc gacgggattg gatttcgagt
     4681 atacagctaa gttatatcaa ttcgatttta aacgttttaa caatgtttca gaatttaaaa
     4741 atgccacatt gttatctgaa acttcgacga tttatgaatt gataacgaat cgcgaagata
     4801 actttaaaca gcatcaaatt ttatatcaga aattgagtga aatgattcat gacacattat
     4861 aattactatg aagcaaacgt tgagaccaat caagttttag tatttctgca tggatttctt
     4921 agcgacagtc gtacttatca taatcacatc gataaattta ctgataacta tcatgtcatc
     4981 actatagact taccaggaca tggcgaagat cagtcttcaa tggatgaaac gtggaatttt
     5041 gattatatta cgacgttgtt agaccgaatt ttagataaat ataaagataa atcaataaca
     5101 ttgtttggat attcaatggg tgggcgtgtt gcattatatt atgcaattaa tggtcatatc
     5161 cctatatcta atttgatatt agaaagtacg tcaccaggta ttaaagaaga agcaaatcaa
     5221 ttggaacgtc gccttgttga tgaagcgcgt gctaaagtat tagacatagc aggtattgaa
     5281 ttatttgtta atgattggga aaagttgcca ttatttcaat cgcaactaga attaccagtt
     5341 gaaatacaac atcaaataag acaacaacga ttgtctcaat cgccacagaa aatggccaaa
     5401 gcattaagag attatggtac aggtcaaatg ccaaacttat ggccgcgcct gatagaaatt
     5461 aaagtaccaa cattaatatt agctggagaa tatgatgaaa aatttgtaca gattgcgaaa
     5521 aaaatggcaa atttaattcc taatagtaaa tgtaaattaa tttctgctac aggtcataca
     5581 attcatgtgg aagatagtga tgaatttgat acaatgatat taggattttt aaaggaggag
     5641 caaaatgact aacagacaat gggaaacact tagagaatat gatgaaatca aatatgaatt
     5701 ttacgaaggg attgctaagg taacaataaa tcgccctgaa gtacgcaatg cgtttacacc
     5761 taaaacagtt gctgaaatga ttgacgcatt ttcacgtgca cgtgatgatc aaaacgtttc
     5821 agttatcgta ttaactgggg aaggtgattt agcattctgt tctggtggtg accagaagaa
     5881 acgtggacat ggtggttatg taggtgaaga ccaaatccct cgcttaaatg tattagattt
     5941 acagcgttta attcgtatta ttccaaaacc agttatcgcg atggtaaaag gttatgctgt
     6001 aggtggcggt aatgtactaa atgttgtttg tgacttaacg attgctgctg ataatgctat
     6061 ttttggacaa actggtccta aagtaggttc atttgatgcg ggttatggtt caggatattt
     6121 agcacgtatc gttggacata agaaagcacg tgaaatctgg tacttatgtc gtcaatacaa
     6181 tgcacaagaa gctttagata tgggcctagt aaatacagtg gtacctttgg aaaaagttga
     6241 agatgaaact gtgcaatggt gtaaagagat tatgaaacac tcaccaactg ctttacgatt
     6301 ccttaaagca gctatgaatg ctgacacaga tggtttagct ggtttacaac aaatggctgg
     6361 ggatgcaaca ttgctttatt acacaacaga tgaagcgaaa gaaggccgtg atgcgtttaa
     6421 agaaaaacgt gatcctgact tcgatcaatt ccctaaattc ccataagtta ttttgaatat
     6481 gatattaagt cacttgcgtc gtttattagc gacgatgggt ggctttttta ttttataagt
     6541 atttaaaagg gcatttttat taattatcat aaagataagt tagtttgaga aaaattaata
     6601 gagaaagaat aaatgttaaa gattattaca atgaaaaaac gagctggtga gaaattgaat
     6661 tctcgccaac ccgcatcaaa agatgaaaat ttaatgcgtt gatgtgccgt atctataaaa
     6721 agaatatgat attgattgtg acttgtatca tcgccatgac gtatacaaat tgatttttgt
     6781 aattgatgat tactccaatt accaatgaat aaattgatat tggtttgttc tagatgtgtg
     6841 agtttggttg tgtcgtaaac taaatttata aattgtagtt gatacatagt tttacctgcc
     6901 tttaatcaat atctatatta ctttttagta acctatcatt gaaccatacc agttataatc
     6961 acgattgaat gataaatgta ataggcttga atctgcatct tggattgata attctgtatc
     7021 ccaaggattc cagtaaataa gtttttcttg gtcattaatt ttagcattag caacaactgc
     7081 tagggcatgt cctaaatgtg ggtcattagg attttgagat acactttgtg caagaatcat
     7141 aatacccaca ttatcttttg taagttgatc aacttgatta tatgatggta cgccttcttg
     7201 ataatgaata tctctacctt gtgatttacc gtattcaatc atttgattag ggaatgttgc
     7261 acaattagga aggtcttgct cacttacttc agggtataat gtacgcataa tatcatgtgc
     7321 attataagtg tctgtatttt tagttgcatt taataacgct gccatactga atcctgcaca
     7381 ccatgagtta tcgaattgtt gttctctaat tttgaagttt tttagtgtgt tttcatattg
     7441 aacttgatct tcttgtatcg cttcgttttc ttcaactttt gtaggtgtta ctgtagtttt
     7501 tagctcttgc tttaattgtg gtgaaactgt tttagcactt tctttttctt tgatgttatt
     7561 agctagtggc gtagctttta ctaatctaac tttgccatct tcttcaaaat aaaatccttt
     7621 ttcatcagta agaacagtga tatttgaatt tttatcttta atttggtcta aatctttagc
     7681 gatgaagttt gaaattttaa cactgtaatt catgtcttct tttgatttgt ttaaatcatc
     7741 tttattttta gggctaagtg ttaaagtata aacaatttta ccgtctttta taactggata
     7801 atagtaacta ttatcttctt cgccattaaa tttataaatt ttaaatgctt caccaagttc
     7861 gtatttacca gtttttgcat tactttgttt atctaatgct ttagcataac cagcaaattg
     7921 ttgttgtgct agatctttta ctttttgagg tactttgtca ctcttaacat taatttctag
     7981 ctgtttagag tgtgaatcgg ctttggcttt attgttatta gcaaatgcgc ctagtgataa
     8041 aataagcatt gaaaccatta tgatgcttat aatattgaat actctagttt tacatgaact
     8101 attcatataa taccctccat ttaagtcaaa atattttgcg ttgttaagtt tttgcttcta
     8161 tatactaaat ccaaattaag atgaagttta tgcagcgtca gggttgtctg aattattgtt
     8221 atcgccattg tccgggttat ttgggttgtt aggttggtca gggttatttg gattgtccgg
     8281 gttgttaggg ttatcaggat tattaggatt gtctggatta tcagggttgt tagggttatc
     8341 agggttgtta ggattgtccg gattatttgg attgtctgga ttatcagggt tgttagggtg
     8401 gtcatcattt gcgaaattga tatcttcaat attttgtttt aagaagttgc gtacattttc
     8461 attaataaat actgcaccgt taaattgatt tggaacgcca ccccaatgaa tgccaatgac
     8521 ttcatttttt tcattaaata caggtgaacc tgagttacca ccagttgtac ttaaatcata
     8581 ttgcattgct tcacctttta agtacgttat ttttccttta ctttcccaca ttgttgcgac
     8641 aggtttatca ccaggatatc ctgttacagt aatattttgg ttaacttgtg tttcagcatt
     8701 attactcatt gttgctggtt taactacttc gccaatatgt ttgttttgct cattagggga
     8761 gaatttaacg attgctaaat caccttcgcc tgaatattta gtgatttgtt cagcagtgaa
     8821 accaccatta ggataattgt cttggttaat tgcagaaggg aatgctttta aagcatgagg
     8881 atcaccgtgc gtagcatcta cgacgtgttt atttgttaaa agtgtatctt tacctacaac
     8941 tacaccagaa gcaataaatg taccagtagg tgcttcaact tgaatataag taacaggtgc
     9001 ataatgacca ttcgttgtat ctgtgatttg gtgacgatcg ttatttggta atataacatt
     9061 agcgcgttca cgttgttcta atggtttaag gttaccgcct ttttgaatct taggtgtttg
     9121 ctgtttgtct gtctgcgttt gttgtggatg attgtccata gcttttgaag ataacgcatt
     9181 tgctgctgga gaactcacaa gtgtcgctgt tgtcaaagtt gcaacgaata aagaactaac
     9241 ttttaaaaat ttacctttca tctaaaaacc tccaaaaaat ttatttacaa gttaaatata
     9301 acactaaaaa tttttaagtc aataagaata tataaataaa ttaaaaagtt tctgtaaaga
     9361 ttagagttta caaaaaca
//
LOCUS       DATTQF010000096         9010 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.096, whole genome shotgun sequence.
ACCESSION   DATTQF010000096 DATTQF010000000
VERSION     DATTQF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.096"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            30..143
                     /locus_tag="VB241_001922"
     CDS             30..143
                     /locus_tag="VB241_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245209.1"
                     /translation="MNGILIKCLNIGGQYYLQIRMLSNEDNAEKTISGNSI"
     gene            525..785
                     /locus_tag="VB241_001923"
     CDS             525..785
                     /locus_tag="VB241_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245210.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVFEKCITLYNVYSRCKRALILSEHSKKNIFNIKKAV"
     gene            795..1292
                     /locus_tag="VB241_001924"
     CDS             795..1292
                     /locus_tag="VB241_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP1245211.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISANIEILL
                     QESSS"
     gene            complement(1400..1492)
                     /locus_tag="VB241_001925"
     CDS             complement(1400..1492)
                     /locus_tag="VB241_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245212.1"
                     /translation="MSVINAYSHASLLFFIYLLFIMYQISLSVK"
     gene            1683..3563
                     /locus_tag="VB241_001926"
     CDS             1683..3563
                     /locus_tag="VB241_001926"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEP1245213.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQETSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTDIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIAEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAHEYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQRQRYQRD
                     VY"
     gene            3629..4237
                     /locus_tag="VB241_001927"
     CDS             3629..4237
                     /locus_tag="VB241_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEP1245214.1"
                     /translation="MSMPLMIDLTNKNVVIVGGGTVASRRAQTLSQYVEHMTVISPTI
                     TEKLQNMVDKGVVIWKGKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVRQ"
     gene            4310..4423
                     /locus_tag="VB241_001928"
     CDS             4310..4423
                     /locus_tag="VB241_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245215.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKIRRTNLIK"
     gene            4726..6096
                     /locus_tag="VB241_001929"
     CDS             4726..6096
                     /locus_tag="VB241_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEP1245216.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVI
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWFGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKPWIKWVNTALTLAVILAMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6343..8193
                     /gene="nrdD"
                     /locus_tag="VB241_001930"
     CDS             6343..8193
                     /gene="nrdD"
                     /locus_tag="VB241_001930"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP1245217.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPNWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYTSG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8190..8726
                     /gene="nrdG"
                     /locus_tag="VB241_001931"
     CDS             8190..8726
                     /gene="nrdG"
                     /locus_tag="VB241_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP1245218.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDKILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            8826..8935
                     /locus_tag="VB241_001932"
                     /pseudo
     CDS             8826..8935
                     /locus_tag="VB241_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3246 a         1251 c         1733 g         2780 t
ORIGIN      
        1 caatatgaaa aataatatga ttaaaaataa tgaatggcat acttataaag tgtctaaata
       61 ttggtggtca atattacttg caaatacgaa tgttaagtaa tgaagacaat gctgaaaaaa
      121 caataagtgg aaactctata tagaatgttc gtttttattt ttaataattg aataatcatt
      181 attgatgatt taatatgtat tcggtcgcac acgatacaaa gtgacttgtt ttaatgatat
      241 gcaataatat gacgtttatc gtcgcgtgta acaatgtatg agtgtgtcag tttaatttta
      301 aaagggttat aaggatattt aataagataa aggagggatt aataaacaat cacatgtgaa
      361 gtttttaagg attaaatgag ttgggcagct ctttaatcca tagttataac gaattttaaa
      421 ttgttagatt taacgtatgg tatcagtatg tatgagttta acttaagtat taatctatag
      481 tttaaagagc ggtctaattg tctttttaaa aatagaaaat aaggatgatt ataatgaggg
      541 gttataacga aagatattgt agctggacta atacagctat acaatgtgat tattttaata
      601 aagtaagaca ggtgaaaaaa gacggtaaat cggataggtt gagcaacaac ataaactcga
      661 acattattta tcgtgtgttt gaaaaatgta tcacgctata taatgtgtat tcgcggtgta
      721 aacgtgcact tattttatca gaacattcta aaaagaacat attcaatata aaaaaggctg
      781 tgtaggggtg tcttatgaat aatagaaatg tgtatgatat cgaagtaagt gattataaag
      841 gcttaactta taaattagaa gcatttagag gtaaagtgat tttagttgtt aatactgcaa
      901 cagaatgtat atatagcgaa caattgaaaa aactagagac tttgtttcaa aaatataagg
      961 atcgtgggtt tgtagtgttg agttttccga ataataattt tgacaatcga caaccaggtt
     1021 ctaatgaaga aatcttgaaa attagtcgtg agaaatttgg tgttacattt ccagtgctag
     1081 ctaaaatatc tgtgaacggg aacgatgaac atccgttatt tacgcattta aagaatgaac
     1141 aaccaggaat ctttgggtcc ccaataaaat ggaatttcac aaaatttata atcgatcgac
     1201 aaggcaatgt tgtaaatcga ttcttgccaa tggaagatcc attggatata tcggcaaata
     1261 tagaaatatt attgcaagaa tcttcatctt aaatttaaaa ttgagcgctt agtatgcaaa
     1321 taacacaaga gcctcttaat gatgtactaa tagtatgtca ttaggaggca ttttatattt
     1381 agaaaatcag taatttgacc tattttactg aaaggctgat ttggtacata ataaatagta
     1441 aataaataaa aaataacaac gatgcatgac tgtatgcatt aataactgac acgacataat
     1501 caaagttttg tctgaattac tgtgtcattc aaaccacatg aaggttgtaa atatttgtaa
     1561 gtataactat cagacacaac tcaagtttgt tttaatttat accatacata ttaaaaaatt
     1621 gactgaacta tcgatgaaaa atgagtcaat catctatttc aagtttattg aggtgaaaat
     1681 atttgaaatt aaatacatct aatagtccat ttacagaaaa acaagtcaca gagattaaca
     1741 atctgctgca aacattaaca gagagccaac aacagtggct tagtggtttt ttactagcta
     1801 atagtaacga tacgacaagt gatagtaatc aacaacaatt agagacagaa gtgtggcaac
     1861 aatcacaaat atcagaagaa caagaaactt caacaacgta tatgttacaa aataaagagc
     1921 cacatatcga agctaatcag cggcatgtca cagtgctata tggttctgaa tcaggtaatg
     1981 ccatgcgttt agctgaaatt ttttcagaac gattaagtga tattggacat caagttgttt
     2041 tgatgtcaat ggatgaatat gatacgacag acatcgcaca gttagaagat ttatttatta
     2101 ttacgtctac tcatggtgaa ggagaaccgc ctgataatgc atgggatttc tttgaatttt
     2161 tagaagacga taacgcacct aatttaaatc atgtgagata ttcagtacta gctttaggtg
     2221 atcaaacata tgaatttttc tgtcaagccg gtaaagatgt agatgcttta ctagaaaatc
     2281 taggcgctga gcgtatatgt aagcgtgtag attgtgatat tgattatgaa gaagacgcag
     2341 aaaagtggat ggcagacatc attaatatta ttgataccac atcagaaggt attcaaagtg
     2401 aatcggtgat aagtgaatca attaagtctg ccaaagaaaa gaaatattct aaatcaaatc
     2461 cataccaagc agaagtatta gcgaatatca atttaaatgg taccgattca aataaagaaa
     2521 cacgacatat agaattttta cttgatgatt ttagtgaatc atatgaacca ggagattgta
     2581 tagtagcatt accgcaaaac gaccctgaat tggttgaaaa actaatatcc atgttaggtt
     2641 gggatccgca atctccggtg ccaattaatg atcatggtga tacagttcct attgctgaag
     2701 cactaacatc acattttgaa tttactaaat taacattgcc attattgaaa aatgcagata
     2761 tctattttga caatgaagaa ttatctgaac gtattcaaga tgagtcatgg gcgcatgaat
     2821 atgttataaa tcgggacttt atagatttaa taacagattt tccaactata gaattacaac
     2881 ctgagaatat gtatcaaatc cttagaaaat taccaccaag agagtattcg atttctagta
     2941 gttttatggc aacgccagat gaagtgcata ttaccgttgg tacggttcgt tatcaagcac
     3001 atggacgtga gagaaaaggt gtatgctcgg ttcattttgc tgagcgtatt aaaccaggcg
     3061 atatagtacc aatttatttg aagaaaaatc cgaacttcaa atttccgatg aagcaagata
     3121 taccggtaat tatgattggt ccaggtactg gtattgctcc ttttagagca tatttacaag
     3181 aacgtgaaga acttggtatg actggaaaaa catggttgtt ctttggtgat caacaccgta
     3241 gttctgactt tttatatgaa gaagaaatag aagaatggct tgaaaatgga aacttaacac
     3301 gcgtagattt agcattttca agagaccaag aacacaaaga atatgtacag catcgtataa
     3361 tggaagaaag tgaacgtttc aatgaatgga ttgagcaggg cgcagcaatc tatatttgtg
     3421 gcgatgaaaa atgtatggcg aaagatgtcc atcaagccat taaagatgta ttggtaaaag
     3481 aacgtcatat ttctcaagaa gaagctgaga cgatattgcg tcaaatgaaa cagcgacaac
     3541 gttatcaacg cgatgtttat taatgcttca atgtgttaaa tgttttaagg tgaaatgacg
     3601 taaaaagata taaaggatgt agcgcaatat gagtatgcca ttgatgatag atttaacaaa
     3661 taagaacgtg gttatagtag gtgggggtac agttgcaagt cgtcgggcac aaacattaag
     3721 tcaatacgtt gaacatatga cggtcatcag tccgacaatc actgaaaaac ttcaaaatat
     3781 ggtagataaa ggtgtcgtca tatggaaggg aaaagagttt gaaccaagcg atattgtaga
     3841 cgcgtatcta gttattgcag caaccaatga gccacgtgtc aatgaagcgg taaaacaagc
     3901 cttacctgag catgcccttt ttaataatgt tggagatgca tcaaatggca atgttgtatt
     3961 tccaagtgca ctacaccgag acaagctaac tattagtgta tctacagatg gcgcaagtcc
     4021 taagttgaca aaatcgatta tggcagagct tgaggcgtta tatccaccat catatagttc
     4081 gtatatcgac tttttatata cttgccgaca gaaaataaag ttactagata taacatataa
     4141 cgaaaagcaa cagttactgt cacaaattgt gtcacaagaa tatttaaatc atgacaaaca
     4201 agctcaattt ttagcgtggt tggatgtaag acaataatag cgggccgtct aaccgtctaa
     4261 ggtaagtctt cttattttaa ctttaacgct taatcattga aattaagaca tgggtggctt
     4321 tgtgaatagt ctaataatga aggatttaag cgataatgat atgcgtttta aatatgaata
     4381 ttacaataga gaaaaaatac gtagaacaaa cttaataaaa tagatggata aattgaaatc
     4441 tggttgaagt cgtgactatc atagcgacct ttagccagat tttttgtgca atagaaagca
     4501 ataataaaaa tgataaatca aaatgaaata caggacagga tatacaagga ttagtcatgc
     4561 catgttatca agtaggaaaa tcaaacttca ctattgatag ttacacaaaa aagaaatttt
     4621 tgataaaatg agttaactta aatataaaaa attatattaa ttattatatt taagttaaag
     4681 agggggatta tgtaaattgt attaaaagtg gagggagaaa ataatatgaa tagtgataat
     4741 atgtggttaa cagtaatggg gctcattatt attatttcaa ttgtaggttt actcattgcc
     4801 aaaaagataa atccagttgt aggtatgaca atcatacctt gcttaggggc aatgatttta
     4861 ggatatagtg tgacagattt ggttggattt tttgctaaag ggttagatca agtcatcaac
     4921 gttgttatta tgtttatctt tgccattatt ttctttggca tcatgaacga tagtggttta
     4981 ttcaagccgc ttgtcaaacg cttaatatta atgacacgag gcaatgtcat tattgtctgt
     5041 gcaatgacag ctttaattgg cacaatagcc caattagatg gtgccggtgc ggtaacattt
     5101 ttgctttcta ttcctgcctt attaccttta tataaagcgt taaatatgaa taaatattta
     5161 ttgattctac tattagcatt aagcgcggcg attatgaaca tggtaccttg gggaggtccg
     5221 atggcgcggg tagctgctgt tttaaaagct aaaagtgtca atgaattatg gtttggatta
     5281 atacctattc aaataatagg tttcattctt gttatgttat ttgcggttta tcttggattt
     5341 aaagaacaaa agcgtattaa aaaagcaata gagagaaatg aattaccgca aacacaagat
     5401 atagatgtac ataaattagt tgaagtatat gaacgagatc aagatgtaag gtttcctgta
     5461 aaaggacgtg cacgaacaaa accatggata aaatgggtga atacagcttt aactttagct
     5521 gttattctag cgatgttaat aaatattgcg ccacctgaat ttgcattcat gataggtgtc
     5581 tcattggcgc ttgttattaa ttttaaatca gtggatgaac aaatggagcg attaagagcg
     5641 catgcgccga atgcattaat gatggctgca gtgattattg cagcaggtat gtttttaggt
     5701 gtactaaatg aaaccggtat gcttaaagcg attgcgacga atttaatcaa agtgattcct
     5761 gcagaagtag gaccatactt gcatattatt gtaggtttac ttggcgtacc attagattta
     5821 ttaactagca cagacgctta ttattttgct gtgttaccga tcgtcgaaca aacagcaggg
     5881 caatttggtg taccatctgt atcaactgct tattcaatgg tcatagggaa tattataggt
     5941 acatttgtca gtccgttttc accagcctta tggttggcaa ttggtttagc agaggcaaac
     6001 atgggcacgt atattaagta tgcattcttt tggatttggg gattcgccat cgttatgtta
     6061 gtaattgcaa tgttgatggg aattgtgacg atttaagtat gaaaaaatag aaactatgct
     6121 cccgttacaa aatgaaataa tagttgcata aagatgtcga aatgacggaa cgattcgaga
     6181 acaattttaa aaattaatga aataattgtg tagaaatatg aatttcacta aatgttaata
     6241 actttgtgac gttttagtta acagactaat aaaaatttga aaatactata tatagtggta
     6301 taacgtaatg agtagacaca atatatagga agaaggggta aaatgaatca aatcgaagaa
     6361 gcattaacgg gtttgatttc taaagatcct gctattgtta acgaaaatgc caacaaagat
     6421 agtgatacat tttcaacaat gagagattta acagcaggta tcgtttctaa atcttacgca
     6481 ttaaatcatt tattaccaaa gcatgttgca gatgcacatc aaagagggga catacatttt
     6541 cacgacttag attatcatcc attccaacca ttaacaaact gttgtttaat agatgctaaa
     6601 aatatgctac ataatggatt tgaaataggc aacgcgaatg taacttcacc aaaatcaata
     6661 caaactgcat cagcgcagct tgtacaaatt atagccaatg tttctagcag tcaatatggt
     6721 ggctgtacgg ttgaccgcgt tgacgaatta cttagtacat atgcacgaca taatgaagaa
     6781 caacatagga atatcgcaaa gcaatttgtc aaagaatctg aaattgatcg ttatgttgat
     6841 caacaagtca ctaaagacat caatgatgcg attgaaagtt tagaatatga aattaatacc
     6901 ttatatacat ctaatggaca gacacctttt gtaacattag gattcggctt aggtacagat
     6961 catttaagtc gcaaaattca acaagctatc ttaaatactc gtatcaaagg cttaggaaaa
     7021 gaccgcacga cagcgatttt cccgaaactt gtattttcaa ttaaaaaagg aaccaacttt
     7081 agtccgcaag atccgaacta tgacattaaa caattagcat taaagtgttc aacgaaacgt
     7141 atgtatccag atattttaaa ttatgacaaa ctcgtagaaa tattaggtga tttcaaagcg
     7201 ccaatgggtt gtcgttcatt tttaccaagt tggaaagatg cggaaggtca ttttgaaaat
     7261 aatggtcgtt gtaatcttgg tgttgttaca cttaatttac ctagaatggc attagaatct
     7321 gccggtaata tgacgaaatt ctgggaaatc ttttatgaac gtatcgatgt gttacatgat
     7381 gcattacttt atcgtataaa tcgtttgaaa gatgctgtac cgaataacgc accaatttta
     7441 tataaaagtg gcgcttttaa ctataaatta aaagaaacag atgatgttgc tgagttattt
     7501 aaaaataaac gtgcaacgat ttcaatgggc tatatagggt tgtatgaaac agctactgtt
     7561 ttctatggtc caaactggga aacatctcaa gaagcaaaag catttacgct tgaaattctt
     7621 aaagaaatga aacgttatca aacgaaatgg acagaattat atgacatttg gttcagtatt
     7681 tacagtacgc cgagtgaatc gctaacggat cgtttttgtc gtttagacca agagagattt
     7741 ggagatatta aagacattac agataaagga tattaccaaa actctttcca ttatgatgta
     7801 cgtaaagatg ttacaccttt tgaaaagtta gattttgaaa aagattatcc ttattatacg
     7861 agtggtggtt tcattcacta ttgtgagtat ccgaaactgc aacacaattt gaaagcacta
     7921 gaagcagtat gggactactc ttatgacaaa gttggttact taggtacaaa tattccgatt
     7981 gatcattgtt atgaatgtga ttacgatgga gattttgaag ctactgaaaa aggatttaaa
     8041 tgcccgaact gtggcaatga taatcctaaa acagttgatg tcgttaaacg aacatgtggt
     8101 tacttaggca atccagttca acgtccagta attaaaggcc gtcataaaga aatttgcgca
     8161 cgagtaaaac atatgaaagc gcctaaagaa tgacactttt agacattaaa caagggcaag
     8221 gttatattgc taaaatagaa tcaaatagct ttgttgacgg tgaaggagta agatgcagtg
     8281 tttatgtatc aggatgtcca tttaattgtg ttggatgtta caacaaagcc tcacaaaagt
     8341 tcagatatgg cgagaaatac actgataaaa tattagcaga aatattagat gattgcgatc
     8401 atgattatat atctgggcta agtctattag gtggcgaacc attttgtaat ttggatatta
     8461 cattaaatct tgtcaaagca tttcgagcac gttttggaaa tacaaagaca atttgggtat
     8521 ggactggatt tttatatgaa tatttagcaa atgattgtac agaacgtcga gagttattat
     8581 catacattga cgttttagta gatgggctat ttatacaaca cttattcaaa cctgatttac
     8641 catataaagg ttctttaaat caacgcatta tagatgtaca acaatcactc tcgcatgcgc
     8701 gtatgattga atatattgtt agttgaatat gtattagaag tcaaggtaac atccgttgcc
     8761 ttggcttctt tttaggttag gcacataatt gaaagttgat aaaagcaatt ctttagaaaa
     8821 acatattgat agaatatgac ctaacaatca ttttgatact aatactaaaa gttgcatata
     8881 cattttaaaa aaagttgaaa gagaaaagtg gtactttagt gggaaggaag tctaactttt
     8941 tggtagcgtt ttacaataaa taaatattcg ttaataacgt ataaatattc ttaaatgcca
     9001 tcctagtaaa
//
LOCUS       DATTQF010000097         8806 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.097, whole genome shotgun sequence.
ACCESSION   DATTQF010000097 DATTQF010000000
VERSION     DATTQF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.097"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..684
                     /gene="modA"
                     /locus_tag="VB241_001933"
     CDS             <1..684
                     /gene="modA"
                     /locus_tag="VB241_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1245219.1"
                     /translation="ADNGKKQEIQVAAAASLTDVTKKLASEFKKEHKNADIKFNYGGS
                     GALRKQIESGAPVDVFMSANTKDVDALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVK
                     DLKGNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAK
                     QGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKE
                     ILKEYHFTA"
     gene            698..1369
                     /gene="modB"
                     /locus_tag="VB241_001934"
     CDS             698..1369
                     /gene="modB"
                     /locus_tag="VB241_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEP1245220.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTILGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGLRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1370..1975
                     /locus_tag="VB241_001935"
     CDS             1370..1975
                     /locus_tag="VB241_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1245221.1"
                     /translation="MLKINLKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2143..3147
                     /locus_tag="VB241_001936"
     CDS             2143..3147
                     /locus_tag="VB241_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEP1245222.1"
                     /translation="MSQERYSRQILFKQIGEIGQNKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IDHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRTACTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENTSITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3176..3682
                     /locus_tag="VB241_001937"
     CDS             3176..3682
                     /locus_tag="VB241_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HEP1245223.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRNFDTDKGGQCVLQLLQVDNV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDVDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNDKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3684..4178)
                     /gene="moaC"
                     /locus_tag="VB241_001938"
     CDS             complement(3684..4178)
                     /gene="moaC"
                     /locus_tag="VB241_001938"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEP1245224.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIYKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFNWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QLDQ"
     gene            4241..5500
                     /locus_tag="VB241_001939"
     CDS             4241..5500
                     /locus_tag="VB241_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEP1245225.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSTMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5497..5982
                     /gene="mobB"
                     /locus_tag="VB241_001940"
     CDS             5497..5982
                     /gene="mobB"
                     /locus_tag="VB241_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEP1245226.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLTNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            5996..6442
                     /locus_tag="VB241_001941"
     CDS             5996..6442
                     /locus_tag="VB241_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEP1245227.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6448..6681
                     /gene="moaD"
                     /locus_tag="VB241_001942"
     CDS             6448..6681
                     /gene="moaD"
                     /locus_tag="VB241_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEP1245228.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFENLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6688..7287
                     /gene="mobA"
                     /locus_tag="VB241_001943"
     CDS             6688..7287
                     /gene="mobA"
                     /locus_tag="VB241_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEP1245229.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7307..8329
                     /gene="moaA"
                     /locus_tag="VB241_001944"
     CDS             7307..8329
                     /gene="moaA"
                     /locus_tag="VB241_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEP1245230.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATADGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8610..8780)
                     /locus_tag="VB241_001945"
     CDS             complement(8610..8780)
                     /locus_tag="VB241_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245231.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDNNVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT         3131 a         1242 c         1741 g         2692 t
ORIGIN      
        1 gcagacaatg gaaaaaaaca agaaattcaa gttgcagcgg cagcaagttt aacagatgtt
       61 acaaaaaaat tagcatcaga atttaaaaaa gaacataaaa atgctgatat taaatttaac
      121 tatggtggat caggagcatt aagaaaacaa attgaatcag gcgcacctgt tgacgtattt
      181 atgtctgcaa atactaaaga tgtagatgca ttaaaagaca agaataaagc acatgataca
      241 tataaatatg cgaaaaatag tctagtatta attggtgata aagattcaaa ttacacttca
      301 gtaaaagact taaaaggcaa tgataaatta gcattaggtg aagtgaaaac tgtaccagca
      361 ggaaaatatg cgaaacagta tttagataac aataacttgt ttaaagaagt tgaaagtaaa
      421 atcgtttatg ctaaagatgt aaaacaagta ttaaattatg ttgaaaaagg taatgcgaaa
      481 caaggttttg tatataaaac tgacttatat aaacaaaata aaaaaattga tactgtaaaa
      541 gtaattaaag aagtagaact taagaaacca atcacatatg aagctggtgc gacatcagat
      601 agtaaattag ctaaagagtg gatggaattc ttaaaatcag ataaagctaa agaaatatta
      661 aaagaatacc actttacagc ataaggagtt gtaatccatg cctgacttaa cacctttttg
      721 gatatcaata cgagttgctg taatcagtac gattattgta acgattttag gtatttttat
      781 atctaaatgg ttgtatcgtc gtaagggttc gtgggttaaa gtattggaaa gtttattgat
      841 attacctatt gttttgccgc caacggtatt aggttttatt ctattaatca tcttctcgcc
      901 aagaggacca attggtcaat tctttgcgaa tgtactacat ttacctgtag tattcacttt
      961 gacaggtgct gtgatagcat ctgtcattgt tagttttcca ctaatgtatc aacatactgt
     1021 gcaaggcctc agaggtatag acacgaaaat gattaataca gctagaacga tgggagcaag
     1081 tgaaacgaaa attttcctca aattaatttt accattagct aaacgctcta ttttagcagg
     1141 tataatgatg agttttgctc gtgcattagg tgagtttggt gctacattaa tggttgcagg
     1201 atatattcca aataaaacga atacactacc tttagaaata tacttcttag tggaacaagg
     1261 tagagaaaat gaagcgtggt tatgggtatt agtgctagtc gcattctcta ttgtggttat
     1321 atctacaatt aatttattga ataaagataa atataaggag gtcgactaga tgcttaaaat
     1381 caatttaaaa tatcaattaa agaatacttt aattcgcatc aatatagatg atactgaacc
     1441 aaaaatttat gcagttcgtg gtccatctgg cattggtaaa actactgttt taaatatgat
     1501 tgccggatta cgtaaagcag atgaagctat tatcgaagtg aatgggcaat tgcttactga
     1561 tacggcaaaa aacgtgaatg ttaaaattca acaacgacgt attggatatt tatttcaaga
     1621 ctaccaactg tttcctaata tgacggtcta taaaaatatt acttttatgg ctgaaccatc
     1681 tgaacacatc gatcaattaa tccaaacttt aaatattgat catttgatga aacaatatcc
     1741 tatgacattg tcaggtggag aggcacaacg tgtagcactt gcacgtgcac ttagcacgaa
     1801 accagattta attttattag atgaaccttt ttctagtttg gatgatacta caaaagatga
     1861 gagtattaca ttagttaaac gtattttcaa cgaatggcaa ataccaatca tatttgtgac
     1921 acattcaaac tatgaagcag aacaaatggc tcatgaaatt attacaattg gttaatgata
     1981 catttgcctt gaaagagttt agaacgtatt taaaaatgta gaagtgcaat cttctattag
     2041 cattttaatg atgttttaaa ctctttttta ggcgcaattt ttgagagaca ttgacgcacg
     2101 tcatataatg aaagtaagga taaatagaaa ggataactta atgtgagtca agaacgttat
     2161 tcaaggcaaa ttttatttaa acaaataggt gaaataggtc aaaacaaaat aaatcaaaaa
     2221 tgcgcgttga ttattggtat gggcgcattg ggtacacatg tggctgaagg tcttgtgaga
     2281 gcaggcattg ctaaactaat cattgttgac agagattata ttgaatttag taatttacaa
     2341 agacaaacat tatttactga agaagatgct ttgaaaatga tgcctaaagt ggttgcagct
     2401 aaaaagcatt tgctagcgtt acgtagtgat gttgatattg atgattatat tgaccatgtg
     2461 gattattatt ttttggagac acatggacag gacgttgacg ttatcattga tgcaaccgat
     2521 aactttgaaa cacgacaact gattaatgat tttgcatata aacatcgtat accttggatt
     2581 tatggaggtg ttgtacagag tacatataca gaagctgcat ttatacctgg taaaacacct
     2641 tgctttaact gtttggtacc acaattgcca gcattaaatt taacatgtga tacagtaggg
     2701 gtcattcaac ctgccgtgac gatggcaaca agtttacaat taagagatgc gatgaaagta
     2761 ttaacggaac aaccaattga cacaaaaata acttatggcg atatttggga aggtagtcat
     2821 tattcatttg gtttcagtaa aatgcaacgt acagcctgta caacttgtgg agatgtacca
     2881 agttatccgt atttaaacaa gaatgaacaa cgttatgcaa cattgtgtgg gagagacact
     2941 gtacagtatg aaaatacatc aattacacac gatattcttg ttcaattttt aaaacagcat
     3001 caactaaatt atcgcagtaa ttcatatatg gttatgtttg aatttaaagg gcaccgcatt
     3061 gttgctttta aaggtggaag gtttttaata catggcatga cacgcacatc agatgccaca
     3121 catctaatga atttattgtt tggataaaaa ggtaaaacaa aaggagtgta atattatggg
     3181 cgaacatcaa aacgttaaat tgaatcgtac agttaaagca gccgtactaa cggtatcaga
     3241 tactagaaac tttgatacag ataaaggtgg tcaatgcgtg ctccaactgt tacaagtaga
     3301 taatgttgaa gttagcgatg cacattatac cattgtaaaa gacgaaaaag tagctattac
     3361 gacgcaggtg aagaagtggt tagaagaaga tgttgatgtc atcattacga ctggtggaac
     3421 gggtattgca caacgtgatg taacaattga agcagtaaaa ccgcttttaa ctaaagagat
     3481 agaaggcttt ggagaattat ttagatattt gagttatgtt gaagatgttg gcacgcgtgc
     3541 attattatct cgcgcagtgg caggtacagt aaatgataaa ttgatatttt caattccagg
     3601 atcaacaggt gcagttaaat tagcattaga aaagctcatt aaaccagaat taaatcatct
     3661 gattcatgag cttacaaaat aatttattga tctaattggc gttgaaaatc tccagattta
     3721 ccgccagact tgctttcaag gtaggtttcg ccaataatca tacctttatc aactgctttc
     3781 gtcatgtcgt aaatggttaa agccgttgct gatgcagcgg ttaaagcttc catttcaaca
     3841 ccggttttgc cagttgtaga gacagttgtt tgaatgttta aagtataaag gggtgcattt
     3901 gtttcatccc agttgaagtg aacatctatg ccagtcaatg gtaatggatg acacatcgga
     3961 ataattgttg atgtattttt ggcagccata ataccagcga tttgagcagt gttcaataca
     4021 ttgcctttgg tattggtgtt attagaaatc tgcttataaa tagtttcatt tacagtaatg
     4081 cttgaatgag cgacagcagt tctttttgta atttgtttgt ctgatacatc gaccattttg
     4141 gcgtggcctt gttgattaat atgagtaaac tcagtcattt tacccctcct agtgcatcta
     4201 gtatatcatg aaaaaataaa agttttggag atgattttta atggtagtag aaaaaagaaa
     4261 cccaatccca gttaaagaag caattcaacg tatcgttaat cagcagagta caatgccggc
     4321 aattacggta gcacttgaaa aaagtctaaa tcatatttta gcagaagata ttgtagctac
     4381 ttatgatata ccaagatttg ataaatcacc ttatgatggt tttgcaattc gcagtgttga
     4441 ttcacaaggg gcaagtggtc agaatcgaat tgagtttaaa gtgattgatc atattggtgc
     4501 aggttcagtt tctgataaat tagttgggga tcacgaagcg gtgcgtatta tgactggagc
     4561 acaaatacct aatggcgcag atgctgttgt tatgtttgaa caaacgattg aactagaaga
     4621 tacatttaca attcgtaaac cattttcaaa aaatgaaaat atatctttaa aaggtgaaga
     4681 aacaacgaca ggcgatgttg ttctaaaaaa aggacaagta attaatccag gggctattgc
     4741 ggtccttgca acatatggct atgcagaggt taaagttatt aagcaaccga gcgtcgctgt
     4801 tattgcaaca ggaagcgaat tattagatgt taatgatgta ttagaagatg gaaaaattcg
     4861 taactctaat ggtccaatga ttcgtgcctt agcagaaaaa ttaggtcttg aagttggtat
     4921 ttacaaaaca caacaagatg atttagatag tggtatacaa gtcgttaaag aagctatgga
     4981 aaaacacgat atcgttatta caacgggcgg agtttctgtt ggagactttg actatttacc
     5041 tgagatttat aaggctgtaa aggcggaagt gttatttaat aaagtagcaa tgcgtcctgg
     5101 tagcgttaca acggttgcat ttgcagatgg aaagtatttg tttggattat ctggaaatcc
     5161 atcagcttgt tttacaggat ttgaactatt tgtgaaacca gctgttaaac atatgtgtgg
     5221 cgcactagaa gtcttcccgc aaataattaa agcaacatta atggaagatt ttaccaaggc
     5281 aaacccattc acacgattta tacgtgctaa agcaacgtta acaagtgctg gagctactgt
     5341 agtaccttca ggattcaata aatcaggtgc agttgtagcg attgcacatg ctaactgtat
     5401 ggtcatgtta ccaggagggt cacgtggttt taaagcgggt catacagtag atattatatt
     5461 gactgaatct gacgctgctg aagaggaact tcttttatga ttttacaaat tgtaggttac
     5521 aaaaagtctg gtaagacaac attgatgagg catattgtct ctttcttaaa gtcacatggt
     5581 tatacagttg ctactattaa acatcatggg catggtaagg aagatattca attacaggat
     5641 tcagacgtcg atcacatgaa gcattttgaa gcgggggcag atcaaagtat tgtacaagga
     5701 tttcaatatc agcaaactgt aacacgtgta gataatcaaa atcttactca aattattgag
     5761 aaatctgtta caattgacac caatatcgta ttagttgaag gctttaaaaa tgctgatttt
     5821 gaaaaagtcg tagtctatcg aaatgaagaa gagttgcaag tattacaaca attgacgaat
     5881 gtttgttata gcattaatgt aagggagcat gaagatttta cagcatttga gcaatggtta
     5941 ttaaataaaa ttaaaaatga ttgtgataca caattaacat agaggattga aatgaatgaa
     6001 acaatttgaa atcgtgacag aaccgataca aacagaacaa tatcgtgaat tcactataaa
     6061 tgaatatcaa ggtgcagtag ttgtttttac cggtcatgtt cgcgaatgga ctaaaggcgt
     6121 caaaacggaa tatttagaat atgaagcgta tattccaatg gctgaaaaga aattggcaca
     6181 aattggagat gaaataaatg aaaaatggcc tggaacgata acgagtattg ttcatagaat
     6241 agggccatta caaatttcag atatcgctgt attaattgca gtttcttcac cgcatcgtaa
     6301 agatgcctat cgagcaaatg aatatgcaat tgagcgtatc aaagaaattg ttccgatttg
     6361 gaaaaaagaa atttgggaag atggttcaaa atggcaagga catcaaaaag ggaattatga
     6421 agaagcgaag agggaggaat aagagagatg aaggtacttt acttcgcaga aattaaagat
     6481 atattacaaa aagcacagga agatattgtg cttgaacaag cattgactgt acaacaattt
     6541 gaaaatttat tgtttgaacg ttatccgcaa atcaataata aaaagtttca agttgctgta
     6601 aatgaggaat ttgtacaaaa atccgatttc attcaaccta atgataccgt tgcattaatt
     6661 ccaccggtta gtggaggtta agcgagcatg aaagcaataa ttcttgcagg tggtcattca
     6721 gtaagatttg gtaagcccaa agcttttgcg gaagtgaacg gtgagacctt ttatagtaga
     6781 gtaattaaga cattagaatc aacaaatatg ttcaatgaaa ttattattag tacaaatgcg
     6841 caattggcaa cgcaatttaa atatccaaat gttgttatag atgatgagaa tcataatgat
     6901 aaaggtccat tagcaggaat ttatacaatc atgaagcaac atcctgaaga agaattgttt
     6961 tttgtcgttt ctgttgatac accaatgatt actggtaaag ctgtaagcac gttgtatcag
     7021 tttttagttt ctcatcttat tgaaaatcat ttagatgtcg cagcttttaa agaagatgga
     7081 cgttttattc caacaattgc attttatagt ccgaatgcat taggcgctat aactaaagca
     7141 ctacattctg ataattacag ttttaaaaat gtatatcatg aattatcaac tgattatttg
     7201 gatgtcaggg atgtagatgc gccctcatat tggtacaaaa atataaatta tcagcatgat
     7261 ttggacgctt taattcaaaa attgtaagcc gttaggaggt ccacaaatgg tagaacaaat
     7321 aaaagataaa ctaggacgtc ccatccgtga cttacggtta tctgtgacag atcggtgtaa
     7381 ctttaggtgt gattattgca tgcctaaaga ggtatttgga gatgatttcg tatttttacc
     7441 taaaaatgaa ctgttaacgt ttgatgaaat ggctagaatc gctaaggtat atgcagaatt
     7501 aggtgtaaaa aaaatacgca ttacaggtgg agaaccattg atgcgacggg atttagatgt
     7561 acttattgct aaattaaatc aaatcgatgg tattgaagat attggtttga ctacaaatgg
     7621 tttgttatta aaaaagcatg gacaaaagtt atatgatgct gggctacgca gaattaatgt
     7681 cagtttggat gctattgatg atacgctatt tcaatcaatc aataatcgta atattaaagc
     7741 gactacgatt ttagaacaaa ttgattacgc gacgtctatt ggtttgaatg taaaagtaaa
     7801 tgttgttata caaaaaggta ttaacgatga tcaaattata ccaatgcttg aatattttaa
     7861 agataaacat atagagattc gatttataga atttatggat gttggtaatg ataatggatg
     7921 ggatttcagc aaagttgtaa ctaaagatga aatgcttact atgatagagc agcactttga
     7981 aattgatcct gtagaaccaa aatattttgg ggaagtagca aaatattatc gacataagga
     8041 taatggtgtt caatttggtt tgattacaag tgtttcacaa tcattttgtt ctacatgtac
     8101 acgggcaagg ctgtcatcag atgggaagtt ttacggatgt ttatttgcaa ctgccgatgg
     8161 atttaacgtt aaagcgttta ttcgttctgg cgtgaccgac gaagaattaa aagaacaatt
     8221 taaagcttta tggcaaataa gagatgatcg atattcagat gagagaactg ctcaaacagt
     8281 tgccaatcgt caacgtaaaa agataaacat gaattatatt ggtggttaat gtgtaggaac
     8341 cactacatat taaatcatta gagattcttt aatatttctg tcttactccc taaaatacaa
     8401 tattatttat taaagtaaaa acggtcatat ctatgccaaa tttaatagaa atgatcgttt
     8461 taaatatttt tacaaattgg cggggcccca acatagaagg tgacgaaaag tcagcttaca
     8521 ataatgtgca agttggcggg gccccaacat agagaatttc aaaaagaaat tctacagaca
     8581 atgcaagttg gggaacaacg aaataaattt tatgaaaata tcatttctat cccactctta
     8641 agaatcacta cataataaat ctttagtggc tctttaacat tgttgtcaca ctccaagtca
     8701 ttgagttgta atatatcttt tttaggtata aatgttgtcg aataaacaac aagttgtcca
     8761 aaagatataa atctaaacaa gatatagcca gcaatttaat atttgt
//
LOCUS       DATTQF010000098         8782 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.098, whole genome shotgun sequence.
ACCESSION   DATTQF010000098 DATTQF010000000
VERSION     DATTQF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.098"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            40..2364
                     /locus_tag="VB241_001946"
     CDS             40..2364
                     /locus_tag="VB241_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP1245232.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQGSKSAYN
                     KDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNTEATLSTNSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNSVPILSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDYVMREDHIVDDNPDNDINTPSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRSTEKRNMAINDHDKLNGQKPNTKTSANNNQKKATSKLNK
                     GRATNNNYSAILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNAVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKELETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLLGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            2381..3739
                     /locus_tag="VB241_001947"
     CDS             2381..3739
                     /locus_tag="VB241_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP1245233.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVVAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            3878..5389
                     /locus_tag="VB241_001948"
     CDS             3878..5389
                     /locus_tag="VB241_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP1245234.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EEIGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLNGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNLEPGQRVMLDQIDSTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYLISCSDNFVVKNR"
     gene            complement(5571..5659)
                     /locus_tag="VB241_001949"
     tRNA            complement(5571..5659)
                     /locus_tag="VB241_001949"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5622..5624),aa:Ser,seq:gct)
     gene            complement(5662..5736)
                     /locus_tag="VB241_001950"
     tRNA            complement(5662..5736)
                     /locus_tag="VB241_001950"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5702..5704),aa:Asn,seq:gtt)
     gene            complement(5878..6447)
                     /locus_tag="VB241_001951"
     CDS             complement(5878..6447)
                     /locus_tag="VB241_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HEP1245235.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            6657..6875
                     /locus_tag="VB241_001952"
     CDS             6657..6875
                     /locus_tag="VB241_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HEP1245236.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYMAEYKNLEAFLGE"
     gene            complement(6956..7942)
                     /locus_tag="VB241_001953"
     CDS             complement(6956..7942)
                     /locus_tag="VB241_001953"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP1245237.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            8141..8317
                     /locus_tag="VB241_001954"
     CDS             8141..8317
                     /locus_tag="VB241_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HEP1245238.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            8332..>8782
                     /locus_tag="VB241_001955"
     CDS             8332..>8782
                     /locus_tag="VB241_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1245239.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSS"
BASE COUNT         3159 a         1210 c         1503 g         2910 t
ORIGIN      
        1 taagtgttaa tgtgtcataa tgaaaagtga ttttattgaa tggagtggac attagtggat
       61 attggtaaaa aacatgtaat tcctaaaagt cagtaccgac gtaagcgtcg tgaattcttc
      121 cacaacgaag acagagaaga aaatttaaat caacatcaag ataaacaaaa tatagataat
      181 acaacatcaa aaaaagcaga taagcaaata cataaagatt caattgataa gcacgaacgt
      241 tttaaaaata gtttatcatc gcatttagaa cagagaaacc gtgatgtgaa tgagaacaaa
      301 gctgaagaaa gtaaaagtaa tcagggtagt aagtcagcat ataacaaaga tcattattta
      361 acagacgatg tatctaaaaa acaaaattca ttagattcag tagaccaaga tacagagaaa
      421 tcaaaatatt atgagcaaaa tactgaagcg actttatcaa ctaattcaac cgataaagta
      481 gaatcaactg acatgagaaa gctaagttca gataaaaaca aagttggtca tgaagagcaa
      541 catgtacttt ctaaaccttc agaacatgat aaagagacta gaattgattt tgagtcttca
      601 agaactgatt cagacagctc gatgcagaca gagaaaataa aaaaagacag ttcagatgga
      661 aataaaagta gtaatctgaa atctgaagta atatcagaca aatcaaattc agtaccaata
      721 ttgtcggaat ctgatgatga agtaaataat cagaagccat taactttgcc ggaagaacag
      781 aaattgaaaa ggcagcaaag tcaaaatgag caaacaaaaa cttatacata tggtgatagc
      841 gaacaaaatg acaagtctaa tcatgaaaat gatttaagtc atcatacacc atcgataagt
      901 gatgataaag attacgttat gagagaagat catattgttg acgataatcc tgataatgat
      961 atcaatacac catcattatc aaaaatagat gacgatcgaa aacttgatga aaaaattcat
     1021 gtcgaagata aacataaaca aaatgcagac tcatctgaaa cggtgggata tcaaagtcag
     1081 tcaagtgcat ctcatcgtag cactgaaaaa agaaatatgg ctattaatga ccatgataaa
     1141 ttaaacggtc aaaaaccaaa tacaaagaca tcggcaaata ataatcaaaa aaaggctaca
     1201 tcaaaattga acaaagggcg cgctacaaat aataattata gtgccatttt gaaaaagttt
     1261 tggatgatgt attggcctaa attagttatt ctaatgggta ttattattct aattgttatt
     1321 ttgaatgcca tttttaataa tgtgaacaaa aatgatcgca tgaatgataa taatgatgca
     1381 gatgctcaaa aatatacgac aacgatgaaa aatgccaata acgcagttaa atcggtcgtt
     1441 acagttgaaa atgaaacatc aaaagattca tcattaccta aagataaagc atctcaagac
     1501 gaagtaggat caggtgttgt atataaaaaa tctggagata cgttatatat tgttacgaat
     1561 gcacacgttg tcggtgataa agaaaatcaa aaaataactt tctcgaataa taaaagtgtt
     1621 gttgggaaag tgcttggtaa agataaatgg tcagatttag ctgttgttaa agcaacttct
     1681 tcagacagtt cagtgaaaga gatagctatt ggagattcaa ataatttagt gttaggagag
     1741 ccaatattag tcgtaggtaa tccacttggt gtagacttta aaggcactgt gacagaaggt
     1801 attatttcag gtctgaacag aaatgttcca attgatttcg ataaagataa taaatatgat
     1861 atgttgatga aagctttcca aattgatgca tcagtaaatc caggtaactc gggtggtgct
     1921 gtcgtcaata gagaaggaaa attaattggt gtagttgcag ctaaaattag tatgccaaac
     1981 gttgaaaata tgtcatttgc aatacctgtt aatgaagtac aaaagattgt aaaagaatta
     2041 gaaacaaaag gtaaaattga ctatcccgat gtaggtgtta aaatgaagaa tattgccagt
     2101 ctaaatagtt ttgaaagaca agcagttaaa ttgctaggaa aagttaagaa cggtgttgtt
     2161 gtagatcaag ttgacaacaa tggtttagca gatcaatctg gtctgaaaaa aggtgatgta
     2221 attactgaat tagatggcaa acttttagaa gatgatttac gctttaggca gattatattt
     2281 agtcataaag atgacttgaa atcaattaca gcgaagattt atagagatgg taaagaaaaa
     2341 gaaattaata ttaaactaaa ataatgttga ggtgaacaac gtgtcaattt ttagccagtt
     2401 tttaaaaaga tcaagccctc aacaaggtat tgtattgtat tatatcgtcg caattgtcgt
     2461 tgcattttta ttattaaact taccgtatgt tcataaacca ggtgtagaag taaatccaat
     2521 tgacacatta tttgttgccg tatccggaat tagtgttact ggattgtctc cgataagtat
     2581 tgtcgatacc tattctacat ttggacaatt aattatcctc gtgatattaa atattggtgg
     2641 aattggcgtc atggcaattg gtacgatgtt atgggtggta ctaggtaaac atattggaat
     2701 tagagaacgt cagttaatta tgttagataa taacaaaaac acaatgagtg gtaccgtcaa
     2761 attgattatt gatattgtaa aatcaatatt tgtaatcgaa ctcgtaggag ccatgttatt
     2821 agcattttac ttttatcgag ataatccaga tttaaaatat gcaatcatgc aaggtgtttt
     2881 tgtttctatt tctgccacta ccaatggtgg attagatatt acaggtaagt cattaattcc
     2941 ttatgcacat gattattttg tacaagcgat agttatattt ttaataattt taggatcaat
     3001 cggcttccca gtattattag aggttaaagc ttacattcaa aatagggtta ctaattttag
     3061 attttcatta tttactaaaa ttacgacatc aacatattta ttcctattta ttgttggggt
     3121 attagccatt ctattatttg aacataacca tgcgttcaaa ggattaagtt ggcatcaatc
     3181 gttattctat tcgctgtttc aatcagcgac tacaagaagt gcgggtcttc aaacaattga
     3241 tgtgacaaca ctaagtgacc ctacgaatat tatcatgggt attttaatgt ttataggttc
     3301 ttcgccaagt tcggttggtg gcggtattcg tacaacaact ttcgctattt taattttgtt
     3361 tttaattaac tttagtaata atgccgataa aacatccata aaagtataca atagagaagt
     3421 acacattatg gatattcaac gttcatttgc agtatttaca atggcgacaa ttttaacatt
     3481 tttaggaatg ctaattatat cagctactga aaatggtaag cttacatttt tacaagtatt
     3541 ttttgaagtc atgtctgcat ttggaacttg tggactatcg cttggtgtca caagtgatat
     3601 tagtgatatt tccaaggtcg tactaatggt actcatgttt ataggacgtg ttggcttaat
     3661 atcatttatc attatgatag caggacgtcg agaaccagat aaattccatt atccaaaaga
     3721 acgtattcaa ataggataat ataatagcaa tctaagttta gttaatgtag attttaactg
     3781 gaacttagat tgctttttta gtttgtattt ttaacttata ttataagacg attggatttg
     3841 aaaatggtaa actagtaaca atgagaggtg taacatgatg gaaaaaaatg aaaacattaa
     3901 tgtagagatt ttaactacgt cagatatgca tagtcatttc ttaaatggtg attatggttc
     3961 aaatatttat agagctggta cttatgttaa ccaagtaaga gcacaaaatc atcgcgtcat
     4021 tttattagat agtggcggaa gtttagctgg ctcgttagcg gcctattatt atgctattgt
     4081 tgcaccttat aaacgacatc caatgataaa gttgatgaac agaatgcatt atgatgctag
     4141 cggtgtgagt ccaagtgaat tcaagtttgg tttatcattt ttaactcgtt caattgcttt
     4201 ggcacgtttt ccatggttat cagcaaatat tgaatacaat gttactaagg agccttattt
     4261 ttcaactcca tattgtatta aacattttgg tgacttaaaa attgctatcg taggcgttac
     4321 agcagatggt ttaatggaaa atgagtattc tgaaatggag caagatgtat ctattgaaaa
     4381 gacattagtg gcatcaaaac gatggattag atatatccat gaagttgaag agccggattt
     4441 tttgattgta atttatcatg gtggattgaa taaaattagt aatagcacga aaaataaaaa
     4501 ggcaagttcg aatgaagctg aaaaattaat ggaagaaatt ggtgttatag atttaatgat
     4561 tacagctcat cagcatcaaa caatagtagg tcaagatcat gaaacgtatt atgttcaggc
     4621 tggtcaagat gcaaaagagc ttgtacatct ttcgattaat tttaaaaaga gaacaacaac
     4681 ttatgatgtt gaaagcattg attctaaagt gattgactta aatgagtatg aagaggacca
     4741 agaattatta gatttaacat tctatgatag aaaagcagtg gcttattggt cacaggaaat
     4801 cataagtgat aaaggtttga tgttatcagt aaatgggtta caagatttag tctgtcaaac
     4861 acatccattt tcgcaattat tacatgatgc aattcacctt gcatttgata atgatataac
     4921 atgtgtccac gtgcctatga acggagagaa ggggttgaat ggacagattc gaaatgaaga
     4981 tttgtatcat gcataccctt atccagataa gccaatggat atgacaatta gtggtcaaaa
     5041 tatcaaagat atattggagt atagttattc acatttagat tttgttaacg agcaattaag
     5101 cttaacaatt attgatgaaa cattatgtac aatgtggcaa ggattcaatt atgagattga
     5161 tatgaatcta gaacctgggc aacgagtaat gttagatcaa attgattcga ctaagagtta
     5221 tagagttaca atgactgact attgttatcg taactacaag aattatttaa aaaatgctat
     5281 tatacatgaa tcatacgatg aaacaatgag tacattaatt gcagagaagt taagagatcc
     5341 gaattatctt atttcatgta gtgataattt tgtagttaaa aacaggtgaa tattgctatg
     5401 taataaatta attataagtt taacaagtat aaaaaccttt cttagagaag attatctagg
     5461 aaaggttttt agaattataa aattgatata aaagaacagt gaaattctca ttcattatat
     5521 atataagtaa acaaaaaagc ttaaatggta tataccattt aagcaagtaa cggaaacgga
     5581 gggattcgaa ccctcgcgcc gctttcgcga cctacaccct tagcaggggc gcctcttcag
     5641 ccaacttgag tacgtttcca atggctccac aggtaggact cgaacctacg accgatcggt
     5701 taacagccga tagctctacc actgagctac tgtggattaa tttgtatgtg tttgaatatc
     5761 aagcacaaat attattataa caagattaag ttaatttgca atagatacct taaaaaaata
     5821 tgcagaacat ttacactata tttataaaac attccgcata ttttaaaaat aaacaattta
     5881 tttatcttca aataaagaat agcgtgtcaa tgcttcaaac acattcaatg tggctttatt
     5941 ataatctatt ggttggtcgg gattttttga tatcatgcga gattgtttat ctaccatgta
     6001 atagtaaaaa atggaattgt tatattggtg ccataaactg tggtatgaaa catgaagaat
     6061 gattgattca ttattaataa atgtcacttt acatttacga ttttttaatt ctttaatact
     6121 ttcgatataa tgcatattta accaaatatt ttcattttgt ctgtcagaat gtgttgggaa
     6181 aaaataagtt ggaaataatg gtgttagtaa aataggtggt ttactagaaa tgccagtaat
     6241 gcggtttgtc tctgctttct tgccaagata agtattacca taaaatttgc aagagcgttc
     6301 aatgattttc tggactttaa aaggattttg aatacgcgtt ttgtcaaacc gaattatttc
     6361 actaccattt ctttgatcgt catcatcaaa tgctggtcga ataaccatgt ctcctttgcg
     6421 tatcacataa atattttgag aatacataag caaaacctcg ctttattaag ttttaaacca
     6481 ttaatataaa aatgcaatta atgatataaa gatattttat aataaaattc tgagttgtca
     6541 agttattatt ttggtgaaaa tgtacaataa tttttaacta atagtcagaa tattcattga
     6601 tttcaaaatt agaaaaatgt atacttatta taaattaaag aaaggtagtg gtttctatga
     6661 aatacaatac taatgttaaa catacaactt tagaagcgtt tgtcacaacc gtcaatgatt
     6721 tgggtattga attaattatc aatgaagcac ttcgagaggt aagaaaacga cagctcatag
     6781 aacttataga tgacgcactc gtcaataaag atgaagcagc atttaatcaa tatatggcag
     6841 aatacaaaaa tttggaggca tttctcggtg aataacattg attcgaattc cataacgcaa
     6901 tcataaaagg actattctta acatctcata catgtaagaa tagtccttta atcgtttatg
     6961 acattaatct aattaattca tgtctatcta tgtcaccgaa atagtgatat aaatcatatt
     7021 ctgatagtgc ttcttctatg tgctcaaagt cgtgtagaca gccaactaat gcattttcaa
     7081 gatcagtgac atctccgaca ccaaagaaat caccgaaaat ttttgcatgt tcgattttac
     7141 ctcgtttaac atcaaacttg atttgtacaa atcctttttc aaatttttcc tcgcgttcaa
     7201 agttatattt agggtttctt ccataattcc attcccaagt tctatatttg tcgttactta
     7261 acttttcaat attttcccaa tcttcatccg ttaatttata ttcttctact tcagtttctc
     7321 caaagatagt tttcaagatg atttttttaa attcttcaat ttctaaaggg tcatttaaaa
     7381 attcttgaat gtttgctact cgtttacgaa cagatttaat accttttgat ttaatcttag
     7441 ctggattaac ttttagtgca ttctgaactt cgtctaaatc actatttaac atcaacgtac
     7501 catgactaaa cattctattt ttaactttaa ccatggcatt tcctgagatt ttcgcttgtc
     7561 caacttgaat atcgttacga ccagttaatt cagcatttac gcctaaagat tgtaatgctt
     7621 gaacaattgg ttcagtgaat ttttggaaat tgtggaaact gttaccatca tcatctgtta
     7681 taaaactaaa gtttaaattg ccagtatcat gataaacagc gccaccacca gaaattcttc
     7741 ttactacatc gatgttatga gcatctatat atgtctgatt tacttcctct atcgtatttt
     7801 gattctttcc aacaatgata gatggtctat ttatgtaaaa taaaaagtaa ctttcttctg
     7861 ctggtaaatt ttttaaaaca tattcttcca ttgctaagtt taaagttgga tctgtaatat
     7921 tattattact aatgaatttc attacaatct ctcccttata tctatatata ttctctactt
     7981 atttatgcct taactttgct gaaaatgcca attatatact cacaaatcgt tctaaatatc
     8041 ggcttaaagt tacattttat tatttttgat tgttaatagt ttatttgtta agtgaatttt
     8101 agtacaatat aatttagtta tgatttaagg aggacttaaa atgactgttg cagaagtggg
     8161 taacattgtt gagtttatgg atggattaag aggtcgtgtt gaaaaaatca acgataactc
     8221 tgttattgtt gacttaacaa ttatggaaaa ttttaatgac cttgatttac cggaaaaaac
     8281 tgttatcaat cataaacgat ataagattgt tgaataagaa ggtaagttat aatgaataaa
     8341 atctcgaagg ctttaacttg gtttattata agtttcatta tatttcatct catattattt
     8401 attatgtggg gcgaacacca agaatactgg tatttatata caggtataat gctaattgct
     8461 ggtatcagtt atgtgtttta tcaaagagat attgaatcta agcggttgct tacatcaatt
     8521 ggtgttggta ttattacggc aattatttta attatgcttc aacttttatt ctcacttata
     8581 acttctaatt taagttatag ttcattaatt aaagaattag caagaacagg tgtcaattgg
     8641 aagtggcaaa tgttagtaac tttacttttt gtcattccat gtcacgagtt atatatgaga
     8701 actgttttac aaaaagaatt aacacatttt tcaataccga aatgggtagc tatattaata
     8761 actgcaattt gttctagttc at
//
LOCUS       DATTQF010000099         8755 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.099, whole genome shotgun sequence.
ACCESSION   DATTQF010000099 DATTQF010000000
VERSION     DATTQF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.099"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            39..431
                     /locus_tag="VB241_001956"
     CDS             39..431
                     /locus_tag="VB241_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245240.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
     gene            complement(560..1936)
                     /locus_tag="VB241_001957"
     CDS             complement(560..1936)
                     /locus_tag="VB241_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245241.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIGIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            2170..3162
                     /locus_tag="VB241_001958"
     CDS             2170..3162
                     /locus_tag="VB241_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEP1245242.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDVTGHSAEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVSIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMMIFKPEKHITIRKLNDNQ"
     gene            3488..4438
                     /gene="lytM"
                     /locus_tag="VB241_001959"
     CDS             3488..4438
                     /gene="lytM"
                     /locus_tag="VB241_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HEP1245243.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHKHYFYNCYPKNANAN
                     GSGQTYVNQATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHNGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(4490..5149)
                     /locus_tag="VB241_001960"
     CDS             complement(4490..5149)
                     /locus_tag="VB241_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245244.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFESNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(5163..6083)
                     /locus_tag="VB241_001961"
     CDS             complement(5163..6083)
                     /locus_tag="VB241_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245245.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGMGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHIPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVILHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLSIFM"
     gene            complement(6080..7246)
                     /locus_tag="VB241_001962"
     CDS             complement(6080..7246)
                     /locus_tag="VB241_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245246.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKQQKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLIIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            complement(7314..>8755)
                     /locus_tag="VB241_001963"
     CDS             complement(7314..>8755)
                     /locus_tag="VB241_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245247.1"
                     /translation="FANTPKNTYLKSEQQTAKMYKDYFNDRFENEVKFQEKMKDNSFL
                     SSLELSADASDEIVKGLGIPKSVVNASKIKMSYGHDPKKEKSMINLEPTIADSALGKF
                     QLAADKDKHYFESPLFKGKYSVNNSDLLSTYSKLTGEDEETAKENGITNQQLNLNTLF
                     SNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKGKKEEIKVNGEKYKVRPVTLTLSRAD
                     TKKITLAVLEEAKKDKDLKKLMEEQGTTKDFEKDIKKAIDDVKETKKDEFAKIQSKIY
                     TEKHTIVKREITITDKENNKTKIKGTNTLEDDKLKLDYALDFDQDKYTYAEAKYTIKG
                     VSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQEKVDGTKRQDKGKITVALDKYSDENE
                     FTFENNIDSDVKNNTQKSTLNIGIKYAEEPINFILKSSTKLKADIDFDDSGAKDFNSL
                     SSKDREKLEKEIEKNGGKMFESILKKASK"
BASE COUNT         2936 a         1382 c         1516 g         2921 t
ORIGIN      
        1 attaaggctt gttaaaactt aagagaggat gtttttaaat gcaattcaaa ttaaaagaag
       61 aagagattat tagtttttta gaattgaaat atccagaaaa agagttcgaa tatggtcgtt
      121 tgttagttgg acaacataaa cgcgatgatt tagatgttta ttactttggc gatacgtttt
      181 taatgtgcac gattatttca ttcaagacat ttgaaattaa agaaacagta gaattatcat
      241 atgatgctgt taatcgtatt gtgttaaaag atggatggtt atttagaaaa atgagaatag
      301 aaacaatgca aaaagtgtta aaatacggta catctaaatt aatgttaact gattttcaaa
      361 aggacaatta taataaatat attcaaggtc agaaacaacg cgtgatattt gaaaatggcc
      421 attttgtcta attgatagtg aataataatt agaataagcg aagaacaatg aaaaatgttc
      481 ttcgcttatt tttatagaat ttgatgtata tcattttaaa gtgataaaga tatttcgaaa
      541 cataattctt ctattatatt taagatttaa ctgtttttgg aatgatcatg tatgcagaca
      601 atgagccgag tatcattaat acaatgctga cgatgaatgt tacagttgca gctacacttg
      661 gtgcatagtt tagttgtaac atactgaaaa ctgtagtact tagtgctata ccaaaggcgc
      721 cacctaatgt accactcatt ttatataatc ctgtagctaa accaactttt tcatttggca
      781 tactgaaaat tgcaattgta aggccgggtg ttgcgactaa accatttcca attgcgcaaa
      841 tgacaaaccc aataacaaca gcaatgatat attgtgatgg ttgaagtgat gtcatgctaa
      901 taatagtgat gccgatgaca gggaacaacg gaccaatgat gagcatcaat ttgccaccga
      961 aacgtaatgt tgctttttca cctaaacgaa tcatcgcaac tgccacaatg gcatatggca
     1021 atgtaacaag tccagattgc gcagctgata aaccaaggtg tgtttgagca tatatgaaaa
     1081 agaccactgt tacgcctaga ccgctattta aaacaaagtt atttaaaaat gcaccaatga
     1141 acggacggtt gcgtaatact gagaaatcaa taaaaggtac ttcatgtcga cgttcgatga
     1201 tgatgaatat cagcgtagtg atgataaaaa tgctcagaca aatgaatgaa aatgtactaa
     1261 accaaccttg ttcgaatcct tgtgttaaca ataatgtaaa gctaccaatc ataacagcga
     1321 aaatcgacat acctttgtaa tcgaatggat gacggtggct atgttgactt actttttcag
     1381 gtgtgccttt tagaagtaat atggcaatga aagcaatgcc tatactaatg atgaaattcg
     1441 tttgccatcc gaaatttgtg gcaattaaac cgccgataac accagctagg ccaatgcccc
     1501 caacagtact aatcattaga taactaatcg ctcgtcttaa atgttctcct ttaaattgat
     1561 tatttaaaac gccaactgtt gaaggtaaca agatagctgc tgatagacct tgtaaaattc
     1621 taccgatgat gagcagtgca gtgatgtccg atataattaa tagaagagat gcaaacatac
     1681 tgattatgag acccatgtat gtcattctca gttgtcctat tttatcagca atatcacctg
     1741 cagctaccat gaagatacct gtggcgaagg aagttaaact aatagataaa tttaacacgg
     1801 caggagaggt ttgatacgtt tgaccaacga gaggtcctat attaataaat gattgtgcaa
     1861 acaaccaata tgttaatgca gacaacataa tcgcaataat aatattactg cgtggtgaag
     1921 attgtgtgtt attcacagat gtcactcctt gtaaaaatta aatatagaaa gtcatgagat
     1981 gaggtgaacg gatgtataga tgatcgttca ctcacacata agtcatgatt tccttacata
     2041 tcataccaca gttattgaga atgaatatca attaattgct tgtatgattg attttttata
     2101 atatgtttaa gtgaaacagc tttatttgaa agtgattaaa gtaaatgaag gtacagagga
     2161 gtgagaacaa tgtgcacagg attcacaata caaactttaa ataatcaagt acttcttgga
     2221 cgcacgatgg attatgatta tccattagat ggttcgccag cagtgacgcc tagaaattat
     2281 cgttggacat ctcgcactgg cacgacaggc caaacccaat atggctttat tggcacagga
     2341 acagatatgg aaggttttat ttatggtgat ggtgttaatg aacatggcgt tgccatttca
     2401 acacaatatt tccgaggtta tagttcatat ggatcaacac acaaagcgga cgcgatgaat
     2461 attacgcaaa atgaaattgt gacatggatt ttgggatata caacaagcat tgaagatatg
     2521 aaacaacaag catcccaaat acatgttgta gcggtatatt taaatgatat cggtgaagtt
     2581 ccgccattgc attatcatgt ttccgatgta actggacatt cagccgaagt ttcatttaaa
     2641 gagggtgaag tggttataaa agataaccct attggtgtct taacaaatca tccagactta
     2701 gattggcatt atagtaattt aagacaatat atcaatattt ctccttatcc agcaacagca
     2761 aagttattgg aaggtgtatc gattgaacct ttaggcaatg aagcaggtac atttggattg
     2821 ccaggtggat ttacttcaac tgagcgcttt gtgagaatgg catttatgaa agcaaacatt
     2881 gctcaaaaca atgataaaga aatggattta atgaatgcat tttatttatt agatgcggta
     2941 aatataccga ttggaattgt acgtccgcat gatgctgaca atcactatac gatgtatcag
     3001 accgtaataa atttaactac aagaacgtta tatattaagt attatggcag caatgaaata
     3061 gtagcattaa agctcacaga tgatctaatt aatagaaaag atatgatgat ttttaagcct
     3121 gagaagcata tcactattag aaagttgaat gacaatcaat agcgattgaa aatggaattt
     3181 ggttgatatg atttgatgga tgattactat catacattag cgttgaaccg cgaacatgga
     3241 cgaatgatta gttgttaatt ttgaaaattt ataaaagctt aagcgaacgc agtgagagat
     3301 gtcattttaa tatcaatgta tggctatttt gtaatgacaa tgtaatgagt ttagtaaaac
     3361 atttcgggaa tattaaatag ttggaaaatg agaattaaat cctttactca gttgtctaat
     3421 tcttttagta tgtgcagtac agtttaattg aaaactaact ttaactttaa tggaggatgt
     3481 tttatacatg aaaaaattaa cagcagcagc gattgcaacg atgggcttcg ctacatttac
     3541 aatggcgcat caagcagatg cagcagaaac gacaaacacc caacaagcac atacacaaat
     3601 gtcaacacaa tcacaagacg tatcttatgg tacttattat acaattgatt ctaatgggga
     3661 ttatcatcac acacctgatg gtaactggaa tcaagcaatg tttgataata aagaatatag
     3721 ctatacattc gtagatgcac aaggacataa gcattatttt tataactgtt atccaaaaaa
     3781 tgcaaatgcc aatggaagcg gtcaaacata tgtgaatcaa gcaacagcag gagataacaa
     3841 tgactacaca gcgagtcaaa gccaacagca tattaatcaa tatggttatc aatcaaatgt
     3901 aggtccagac gcgagctatt attcacatag taacaacaac caagcgtata acagtcataa
     3961 tggtaatgga aaggtcaatt accctaatgg cacatctaat caaaatggcg gatcagcaag
     4021 taaagcgaca gctagtggtc atgcgaaaga cgcaagctgg ttaacaagtc gtaaacaact
     4081 acaaccatat ggacaatatc acggtggtgg tgcgcattac ggtgtcgact atgcaatgcc
     4141 tgaaaattca ccagtttact cattaactga tggtacagta gtacaagcag gttggagtaa
     4201 ctatggtggc ggtaatcaag taacgattaa agaagcgaac agtaataact accaatggta
     4261 tatgcataat aatcgtttaa ctgtttcagc tggtgataaa gtcaaagctg gtgaccaaat
     4321 tgcatattca ggtagtacag gtaattcaac agcacctcac gtacacttcc aacgtatgtc
     4381 tggtggcatc ggtaatcaat atgcagtaga cccaacgtca tacttacaaa gtagataata
     4441 cagtaaatcc caagttacga tatcatacgc aacttgggat tttttcgttt taataatagg
     4501 tataagccca ttgcttgtcc tctaaaacat gttgataaaa aggatcttgg ccaattaact
     4561 tgatatcatc tgcaagtgct tcaacttcat ctaaatgatg ggtagttaat ataattaaac
     4621 atttagattt cataatgtta agtagttggt ggatgtcatg tctagatttt aaatcaatac
     4681 caactgtcgg ttcatctaaa ataagaattc gaggttgacc tagtaaacct actaatatat
     4741 taattttacg tttattccca ccggacaatg tagatacttt ggcagacgta tcatcaaagt
     4801 ttaattgctg taaatattcg ttgatagttg tatcgttaat tggattttta caaagtgatt
     4861 taaaaaattt aatgttttca gccactgtca tgtgctcaaa taacgcaatg tcttgtggca
     4921 cataaccgat gtgattttgt atttgtcttt gattccattt ttcgccgaaa tagttgatag
     4981 ttccatcatt agctttttca ataccagcaa tcatacgaag taatgttgat tttccagcgc
     5041 cattatcacc aagtaatacg gttaaacgat tactttcaaa ggacatggtt aaatgattga
     5101 aaatctgttt gttgcggtaa cgctttgaaa ggttattaat ttctatcatc agttacgctc
     5161 ctttacatga aaatgctcaa gtatacgata cccatagcaa gtgcatatat aaatgtcatg
     5221 aataatcgat ggcttattgt ttgaatatgg aataagataa agacgatacc tatctcataa
     5281 atcaatataa gtagcagtga ttttaagtaa aatattaagc tgagtggttg agacaaatat
     5341 agactaactg ccaatagtac caacaataac aaaatagtat gtgtcattac ataagtacta
     5401 tatagtttga aacggcttaa atgatattgt gataatcgtt gcaatgctgc ttgttggttt
     5461 aaacgataat gaagtattac ttgaacagcg ctaacaaata aaatcaccgc aaagattaag
     5521 ctaattgaaa tagagtgttg tgcttgttta gtaagcgaca caaatttgat tttagattca
     5581 ggtatatgtt tatgatagga cttgttgata gcatcgatgg attgatgctg tttcatatcc
     5641 tcaaggtgtt cataaataat gttaggaatt tgctgttcat ataatgagct actgacaatt
     5701 tctacagcaa taccacctat aaagtcatct ctaccatata actgtatcgt ttctttcaaa
     5761 cggttctctt ttaatttttg agagaaacct ttaggaattt gcatacttaa aatagcttcc
     5821 tttttagtaa catcatcttc aatatagctt tcatcttcat cgactttttt aatagttaca
     5881 tagtcagatt gtttaatttt attgacgaat gattttgatg cagtggtttg gtctaaatct
     5941 tgaatggtaa tcggtatttt gaagttgtca tgtgctacac ggtaacccat accaataagt
     6001 acgagtgcga tgacaatggt tgttacgagc aagatgtatt gtaaccattg cttgaacaca
     6061 acaagttgta tataaggctt cattgacgat acctccaaac caatacagct aaattaatta
     6121 tcaaaagtgc gatgaagcta agatagaaac tagggtgcag ttctaaaatg tagttgttta
     6181 aaataatttc taacaattga tttgttacaa ctgcgaacgg ttgaatattg aaaacgccat
     6241 ttgctatatg ttgtaaaaaa atcgtaggta ttgttaaacc agataacacc aggatgacaa
     6301 tagctaatat gactttacta atactattca acaagcctgt tgttaaaagt tcgatgatta
     6361 ataaccacag tattaaaaag gtaacataat agcttaaatg aatggctaac gttggccaat
     6421 tatataattc aaaggtattc ggaatactga acacaatcca aactacacca acgatactcc
     6481 ataacatagt ataaaaccat gttatcaacg cacgaatgat taataaacgc tctttagaaa
     6541 aatgaaacat tttcaatcgc gctttcaata cagtatcttg attcattttc aaaactgtaa
     6601 ataaagatag tgcaaagatg aataccgttg ttaaaaatcc tgtaattgca taataactgc
     6661 ccgtatcgta taaatgaatc ggttctaagt taaatgcacc tgaacggttt aatcctgtaa
     6721 tcagcaaatc agtcataaca ttgatactgt cagaatgtga tgctttcggt gctaggtctt
     6781 gaaaagctaa gatgccaccc attgaccgca taaggcgttg gtaaacagaa tctgttagct
     6841 gagatagcac gacacttttc atggattgtt gatcatatgt atatactgaa attggtagtt
     6901 caccttgttt ataaaatgcc ttggtcatac ctttatcaaa aacaaaatag ccttgaagtt
     6961 tctgctgttt tagcaaagta tgtgcttgct tatcatcata tgctttaatg ctcacatttt
     7021 tacctaggtt actcccttta ccaatagagt ttaagattaa tttcgtttca cttgattggt
     7081 ctttatctac gacacctata ttaaaatgat tgtcatcttc tgttacatgt tggattgtcg
     7141 ttaatgtaat aaggagtgcc gctaatataa atagtaaata gataatcaaa taccactttt
     7201 tcaataaaaa agagtggtag atgcgaaaca aatgtattgt tttcatttaa ccactcctcc
     7261 aatgataaga ttgaaaggca agatgacctt ccaatcttat ttttatattt tagttattta
     7321 gatgcctttt ttaaaattga ttcaaacatt ttgccgccat ttttttcgat ttctttttca
     7381 agtttttcac ggtcttttga agatagacta ttgaaatctt tcgcaccact atcatcaaaa
     7441 tcaatatctg ctttcaattt tgtgctagat tttaaaatga aattaattgg ttcttcagca
     7501 tatttgatgc cgatatttaa cgtagatttc tgagtgttat tttttacgtc agaatctata
     7561 ttattttcaa aagtgaattc attttcgtcg ctatatttat ctaacgcgac agtgatttta
     7621 cctttatctt gacgttttgt gccatctact ttttcttggt tatctaattt gatttttgat
     7681 tcatcatatt ctgtcttttt accaaattcg tatttatcac tgtatttatt gtctttttcc
     7741 ttagaagata cgcctttaat tgtatatttc gcttcagcat acgtgtattt atcttgatcg
     7801 aaatcaagtg cgtaatctag ttttaactta tcgtcttcta aagtattagt acctttgatt
     7861 ttagttttat tattttcttt gtctgtaata gtaatttctc gttttacaat cgtatgtttt
     7921 tcggtataaa ttttagattg aattttagca aattcatcct ttttagtttc tttgacatcg
     7981 tcaattgctt ttttaatgtc tttttcaaag tcttttgtag taccttgttc ttccattaat
     8041 tttttaaggt ctttatcctt tttagcttct tctaatacag ctaatgtaat ttttttagtg
     8101 tcagctctgc taagtgttaa cgtgacaggt ctaactttgt acttttcacc attaacctta
     8161 atttcttctt ttttaccttt atcaaaatta tcgtcatcta atttgtcgac aataagttcg
     8221 gaatattttt cggcaatttt gctgtagtca ctttgttgtg cttgagcatt actgaaaaga
     8281 gtatttaaat ttagttgttg gtttgtaata ccattttctt ttgctgtttc ttcatcttca
     8341 cctgtaagtt ttgaataagt tgataataaa tcagaattat taacactata tttcccttta
     8401 aataatggtg attcgaaata atgcttatct ttatctgcag ctaactggaa tttccctaat
     8461 gcagagtctg ctattgttgg ttcaagatta atcattgatt tctctttttt aggatcatgt
     8521 ccatatgaca ttttaatttt cgaagcatta acaacagatt taggaatacc aagcccttta
     8581 acaatttcat cagatgcatc tgcgcttaat tctaatgaag ataaaaatga attatctttc
     8641 atcttttctt ggaacttcac ttcgttttca aaacggtcat taaaataatc tttatacatt
     8701 tttgcagttt gttgttcact ttttaagtat gtatttttcg gtgtgtttgc aaaaa
//
LOCUS       DATTQF010000100         8587 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.100, whole genome shotgun sequence.
ACCESSION   DATTQF010000100 DATTQF010000000
VERSION     DATTQF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.100"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(148..963)
                     /locus_tag="VB241_001964"
     CDS             complement(148..963)
                     /locus_tag="VB241_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEP1245248.1"
                     /translation="MSFDVFLYFKTGKRYVAIFIISFSFYLLNQPNNWAWSSLNICIN
                     DSKPSNVKVAILDTGINNTRINNNFVTKRYNAINNHNQITTDSNHATQVASIITYGEH
                     KEKLIGISKSVSIYDVKVLDDNLKGDIDNIIKGITWSIKNDVNIIHMSFGFQRYNKEL
                     HDAIKNAKNKGIILVAAAGNNMDDYSDYPARFNEVYSITAIDKNNKPFIYAPTKKIDF
                     KAPGVDVYTKGSKDELLTVNGTSFAAAYFTAYLTNNLEFRNDLPHILKEYPIK"
     gene            complement(1005..1697)
                     /locus_tag="VB241_001965"
     CDS             complement(1005..1697)
                     /locus_tag="VB241_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEP1245249.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDTVSTVDIVI
                     GRVAQVHIDDKFILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPSASKEELAGLE
                     GRNFDNQSDNKE"
     gene            2011..2954
                     /locus_tag="VB241_001966"
                     /pseudo
     CDS             2011..2954
                     /locus_tag="VB241_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2941..3243
                     /locus_tag="VB241_001967"
     CDS             2941..3243
                     /locus_tag="VB241_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245250.1"
                     /translation="MRTNNHITHLDAIELSSGWNYNSKLVAGKNALLSDSLLRDIKVL
                     SSNAKGDADMLETELHAVINAMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            3393..3752
                     /locus_tag="VB241_001968"
     CDS             3393..3752
                     /locus_tag="VB241_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245251.1"
                     /translation="MILRYVTNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSAAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(3837..4448)
                     /locus_tag="VB241_001969"
     CDS             complement(3837..4448)
                     /locus_tag="VB241_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEP1245252.1"
                     /translation="MHTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGNFKINPHALQAVSRLAGNDYAKLGGIFTIDRPEK"
     gene            complement(4527..5003)
                     /locus_tag="VB241_001970"
     CDS             complement(4527..5003)
                     /locus_tag="VB241_001970"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEP1245253.1"
                     /translation="MAVVYVAGGCLWGVEAFFTTIPGIIHTEAGRANGRSSKLDSPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNRQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDKIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISIDLLNKYK"
     gene            complement(5006..5506)
                     /locus_tag="VB241_001971"
     CDS             complement(5006..5506)
                     /locus_tag="VB241_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245254.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATPEALKLRIVQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVPANNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(5741..6508)
                     /locus_tag="VB241_001972"
     CDS             complement(5741..6508)
                     /locus_tag="VB241_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEP1245255.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(6505..7197)
                     /locus_tag="VB241_001973"
     CDS             complement(6505..7197)
                     /locus_tag="VB241_001973"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEP1245256.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIEDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMHKDKS
                     ASYYAYYGTDES"
     gene            complement(7214..7876)
                     /locus_tag="VB241_001974"
     CDS             complement(7214..7876)
                     /locus_tag="VB241_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HEP1245257.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2816 a         1485 c         1239 g         3047 t
ORIGIN      
        1 atttgtgttt attttactat tcgaattgtt aatattagct tcttttataa ccacagaagc
       61 ttctgcaaag tttccgtaag taaaagttat aataattgct agaaatagga taattatctt
      121 gtttttcatg ttttctcctt tgatgcgtta ttttattgga tattctttta aaatatgtgg
      181 taagtcattt cggaattcaa gattatttgt cagataagca gtgaaatagg cggctgcaaa
      241 agatgttcca tttacagtaa gtaactcatc ttttgatcct tttgtatata cgtctacgcc
      301 aggagcttta aaatcgattt ttttagtagg tgcatatata aatggcttat tgtttttgtc
      361 tattgctgtg atagaatata cttcgttaaa acgtgctgga tagtcactat aatcatccat
      421 gttattccct gctgcggcta ctaagataat acctttattt ttagcgttct ttatagcatc
      481 atgtaattct ttattgtaac gctgaaagcc aaaactcata tgaataatat ttacgtcatt
      541 tttgatactc caagttatgc cttttattat gttgtctata tcgcctttaa ggttatcatc
      601 taatacttta acgtcataaa tacttactga tttactgata ccaatcaact tttctttatg
      661 ttctccatat gtgataatac ttgctacttg agtggcgtga ttactatctg tagttatttg
      721 attatgattg tttatagcat tgtatctttt tgtaacaaaa ttattgttta ttcttgtatt
      781 attaataccc gtatctaaaa tagcaacttt tacattggat ggctttgaat catttataca
      841 tatatttaga ctgctccatg cccaattatt tggttgattt aataaataaa acgagaaaga
      901 aattataaat atagcgacat atcgcttccc ggttttgaaa tataaaaata catcgaaact
      961 aatttcaatg tgtagtttcg atgtattgaa aatgattgaa ttcactattc cttgttatct
     1021 gattgattat caaaatttct tccttctaaa cctgcaagct cttctttaga agctgatggt
     1081 gctttcattt caaatatttc attcacaacc gtgtaatcat aatatcctaa cctagcaata
     1141 ggtttaatcg acttaatgtc taatttacca ttatcaagaa tgaacttatc gtcaatatga
     1201 acctgagcaa ctcttcctat aacaatatca acggttgata cagtgtctcc tgtcggaata
     1261 cgaatcgttt gaacgtactc acattcaaaa tgtactggcg attctttgac tcgataacct
     1321 ggtgcttcta tacatgcttc tttcgtcaca cctgcatatt caaattcatc ctcttcaggt
     1381 ggtagtgcct tcgacgataa attaacagct tctcttaaat catacgtggc catgttccat
     1441 acaaaccacc ctgtctcttc tgcatttttt actgtatctt tacgctcatg atcaccaaga
     1501 acagattgat ttgctgcgaa cataaccata ggcggatccc atgttaagtt ttgatactga
     1561 ctataaggtg ctaaattatc tttcccatct ttcgatacag tagagatcca ccctattgga
     1621 cgtggtactg tactactttt aaatggatcg tgtggtaagc catgactcct tacaccttgc
     1681 ttcggcgaat aattcattct atcctcaccc cttattagta attacattta aggttacgct
     1741 ctcatgacat aagcgtctaa tataaataat caatttattt ataagaagaa actatatatg
     1801 acgtggttgc ttataatttg tgttcttgat tcgaaaattt tagataagga tttatgcaat
     1861 taatatttaa tgatgttttt tgttaattta attaatatta gttaatataa tcaagattag
     1921 cttataataa tattgaataa cattatttta gtttttgtat aatttgccta tcaactgaaa
     1981 aacagctaat acaaaggagc taatgaagat atgagaaaaa ttatgcgaat cttattatgc
     2041 acatcaatca taataagcgg ttgctctttc atatcaaaga accaaacaaa agatattcca
     2101 aagacggtta gcgtcaaaaa ttatgatggt aaatatattg gtgaacataa aaaaagaaat
     2161 gaagtatttc tgaaaaaaca taaaggtgaa gcgataaaaa aatataaaga ctatgtgaaa
     2221 gatacatttg gttatgattg taaaattaat ttagttgagg cctatactaa taaatctgga
     2281 tttagcgaaa aagtaaggta gatggtttag tagtcgtcgg tactgtcaat tatgacgttc
     2341 cctttcaatt tagactgata tttgttgagt ctggaaatgg catagctatc acaacattta
     2401 cacctggtca tataaatgaa acatcagcgg cagttgcagc catgatgtat aagcgttatg
     2461 aacctgaaat tgaacaagct cgattaaaat tcaaatcaga agttgagaaa aacggttatt
     2521 atgccatgaa tgaaaaactt caaaagaaac aagaattcaa tggtgttact aaacaatttt
     2581 tgaatttcaa cacagacagt atcgatgatt tggataagtt caaaaaagaa ttcaaacctg
     2641 ttatgcactt aaaaggagat gcattaaatc aacaattaca atctttgatt aataaatatc
     2701 ctcaaataca aaaaaatatg aagtccgagt ttattgccta ttacgataaa ggtgagaaca
     2761 gagagactgt tgctaactat gtttggaacc tacaaaaatc tataaatgac gttatgcaat
     2821 catatcctag cacaaaattt gtacagtttt ataaagatga tgtttccccg tcaatggtag
     2881 atggaaatgg ccgtttaaaa tcggatacta atgtaatttc catcgaggga ggaaagtata
     2941 atgagaacaa ataatcatat aacccattta gatgctattg agttaagtag cggttggaat
     3001 tacaattcga aattggtagc tggcaaaaat gcactactat cagatagcct tttaagagat
     3061 attaaagtat taagttctaa tgctaaaggt gacgccgaca tgttagaaac ggaacttcat
     3121 gctgttataa acgcaatgtc tcaaaacgaa ggtaaatctg ttgatgcact taaaaatagt
     3181 accgcttctc taaaaaacaa tctaaccaaa ttaaaagagc aactcgataa aactacacat
     3241 taatttcata cattttgagt gaacagaatt atatttccat agcaaacatt cctgaactac
     3301 acatgttaca taagagattc atttttgaag aaacgggtaa aatgataaaa caaccgtttt
     3361 tcaaaaaatc aaatattggg aggaataaaa atatgatttt acgttacgtg accaatttaa
     3421 aacttgcgaa agagctatac caagccagta aacctaaatt gcaagaagat aagggtatga
     3481 ttgaactgtt tgaacatact tttggtttgc aaaaagaact tgtaaaatac gtcggcattg
     3541 cagaagcaac tactgctgct ttatactcag ctagctttat caataaaaac atttctcgcc
     3601 ttgcttcgct atcaacaata ggtattttaa gtgcagctgc ttacaaacat ttcgaagctg
     3661 gccaaggtaa aaaaggtgca caacatgcat tagatttatt aggtcttgct actttaagtt
     3721 tactagatac attttcttgt aataaaaaat agtcttcatt ttagaagcac atactttagc
     3781 acctgtgtac ttttgataca atccataaac ggtacaccgc tatagcatgt gttttgttat
     3841 ttttcaggtc tatctattgt aaatattccc cctaatttag cataatcatt tccggctaaa
     3901 cgagaaacag cctgtaatgc gtgtggattg attttaaaat taccctcata aatactgtca
     3961 tcaatatgat aagttacaat ttcgcctatt attaagtcag ccccatctaa tacatctcca
     4021 agcaatatca tttgcgatag tttacattcg aatctcattt tcgcgtcttt aattcctggc
     4081 gtcttaatcg ttgtagatgt taaaagtgat aattctgtac gactcaactc actgtcacca
     4141 tatgctaacg gcgctgcagt ctcattaata tcttgaacat tatcttcgtc tgtaatatgc
     4201 acaacaaagt ctccagtccg ttctatattc aatgcagtat cttttctctt acctcctgca
     4261 cgttgaactg caatagcaat cattggcgga tgattattaa ctatattaaa aaaactaaat
     4321 ggtgctgcat ttactgatgc atcttgattt aatgttgtaa caaaagctat aggtcgtgga
     4381 ataattgaac caattaataa tttatagttt tctctagcag ttaatgattg tgcatcaaac
     4441 gtatgcataa tacctacctc ttttcttagt atatctaggt atttctccga tttcggttaa
     4501 tttaaacatc tattctcctc tgaaaatcac ttgtatttat ttagcaaatc tattgaaata
     4561 tgacacatat gcatatcttc tggatatttt tctaaatgtt gctgatgttc ttcagcactt
     4621 ttaatgtagt tagacagcgg taagacttcc actgcaattt tatctctgtc tttacgtcgt
     4681 tcaatgaact gacgcgcttc aattaagtgg tcatctacac aactatataa acccgttcga
     4741 tacttttctc caatatcatt tccttgtcga ttcacactgt atggatcaat aatttcaaat
     4801 aaataattca tgatgtctgt aattgttaac atacgatcat caaaatgaag tttgacacat
     4861 tcagcataac catcatacgg actgtctaat ttagagcttc ttccatttgc tcttcctgct
     4921 tctgtatgta taattccagg tattgttgtg aaaaatgctt caacacccca taaacaccct
     4981 cctgctacat aaacaactgc catatttaca cctcatcatc cttttttata tttttaacaa
     5041 ggttatacca tttaataccg ccatgacatg attctgatac accttcatta cgataaccat
     5101 atttttcata aaaagaaatt aatgattctc gacatgttaa cgttacacca tgtcgatgtt
     5161 gattcttagc aagattttca aaatagttta gtaagcgacc tgcaataccc tgaccttgat
     5221 aatttggtgc tacaacaaga cctaacacac taatataacc accttcacta ttatttgcgg
     5281 ggacattttt aaataaatca tcgctaatgt aacgctcttt tatgacagga ccgttgatat
     5341 atccaatcac ttcatcattc ttttcagcta caataaaagt ttcttgaatt tgtacaattc
     5401 tcaatttcaa tgcctctggt gttgcagctt cttccggtga aaaaccaata ttttcaagtg
     5461 caataatttg atctaagtcc tgcatgatta cttttcttaa taccataaaa acacttcctt
     5521 agccttatat atcaaatata ataccttcat cattatattc attattttca aactaccttc
     5581 actataaaaa acgagtcttt ttattaaaag ccattcacct taatcaatta gacaagttgt
     5641 attatacgaa ttagcaaata atcataatta taattcattt taagcatact caaaaagact
     5701 ggtacatgta taccagtcta attcgaaaaa atatatttag ttaaaaccat ttcaaaaacg
     5761 acttcacttt tcctggctta gtcggtctgt ctagataaac aaaattatca ttgattactg
     5821 aactggcatt tcgaagtaat gcttcaatgt cattcgaata tttcttcaat ttatgattgt
     5881 gaaataattc ttgcatcaaa aatggtcttt ggtcacatga atgtgcatct gaagctacaa
     5941 aatgagccaa attacattct ataaattgta atgataactt ttgaatgttt tttccaaatc
     6001 caccaactaa agaactcgat gttaattgac tcaatgcccc atttgcaaca agttcatata
     6061 atatttctgg atttttggcg atacttctat ttctttcagg atgtgcaatg attggtatgt
     6121 agcctctcga ttgtatttca aaaaacaatt gttttgtata atgtggtact tcgcccgttg
     6181 gaaattcaat tagtaaatat ttcgaacgat taataccttg aatactacca ttatctaagc
     6241 cttgcagaat cgaatctgta attctaattt cttgcccggg aaataattta atatccaatg
     6301 cttgaacttc tggatgcgtt cttaactccg ccaatttcac aagcacttgt tgaaatgtat
     6361 tatcatatct cggatgcaaa tgatgaggtg tcgctacaat acttgttaca ccttcatcct
     6421 tagcttgctt taatagtgca atactctttt caattgtctt aggaccatca tctatatcaa
     6481 ctaatatatg gttatgaata tcaatcatga ttcatcagtc ccataatatg catagtaact
     6541 agcactttta tctttatgca ttctatttaa gactacacct aataatttag cacctgttgc
     6601 ttcaataagt tcttttcctt ttttaacttc atctttatta ttattttccg aattaactac
     6661 gtagacaaca ttgccggtaa actttgaaaa taattgcgca tctgtaactg tgttcactgg
     6721 tggcgtatcg ataattacaa agttataatt catcaataat gtgtcataca aattcgcaaa
     6781 tgctcttgat gtgattaact ctgatggatt tggcggaatc ggtccagacg tcaggacgtc
     6841 taaatcttca atttcagttg agataatact gtcttgataa gttgaccaat ttagcaataa
     6901 acttgatagg ccttcattat ttggcaaatt aaaaatatag tgctgcgtag gtttacgcat
     6961 atccccgtct acgattaatg ttttatatcc tgcttgtgca tatgcaactg ctaaatttgc
     7021 tgcaattgta gacttacctg cgcctggtgc ctctgaagtg attacaatgc tttgaactgc
     7081 actgtcagga tttgcaaaca taatatttga tcttatgcct cgaaatttct cgctaatagg
     7141 tgactttggt tgttcatgga caattaaact tgatgtactt cttcgtgtat tcgtcatggt
     7201 aattcctcgt aaattaaaat ttttgtattg aacctaaaat aggtaatcct agttgcgatt
     7261 caacatcttc ttccgtctta atacgcttat ctaataattc ttttaagaaa ataatcaata
     7321 ttgctaaaac aataccaaca atgatactga ttactaagtt aacagatact attggagata
     7381 cttttacagc attatcatgt gctgaggaaa gtattgtaac attatcaaca ctcataattt
     7441 taggcatgtc atgtgcaaaa actttagata ttctattaac aattttgtca gattcagatt
     7501 tattcccagt tgtaactgat acagtaataa tttgagagtt tgtttgattg gttactttta
     7561 aaaacgaatt caactcagct gttgaatact gaccatcaaa ctctctagat actttatcta
     7621 gaattcttgg acttttgata atttccgtat atgtattaac agactgcaaa ctactttgaa
     7681 cattttggaa agctaaatca cttgaggact ttttcatgtt tactaatatt tgagtagaag
     7741 cagtatattt gtcaggcata acaaaaaagg ttaatgccgc acttactaca agacatattg
     7801 ccggtaaaat aagcaataat ttaatattct tctttagaat atttaatagt tttactaaat
     7861 caaacttttc tttcatggtt tcctccacat aatcaatcat tgtattcatt atgtatgttt
     7921 tataaatcgg acaattatat ctagtttaac gaccacaaaa atacacaact acattttctc
     7981 taatttttta tataaatatt ttatcgttta aaattatatc atgattcttg gtcgttatgt
     8041 ataatttatt tatattttcg tttgagatat aatattgccc aacttcaaat atccaaacct
     8101 atcagtaata aacctagata ccatcgtctc tattatggct ttcttataat cgagtagaag
     8161 cagcatcatt acttgattat ttgctcttta caacaccgag cgtgcccgta ctcggtaatt
     8221 caatactttg cgtaacccgt cactgtgagt tgggttaatg atgataaagc ccacatcttt
     8281 taaaaagatg tgggtaattt atataatttt tatttacatt ttaaacttat aaaaaaagcg
     8341 cctatgtcat gatttaccat cacataggcg cttatcaata aattattaat tattactttc
     8401 catttcatct aatttatgcg gatttcctgt aattagatga caacttattc ttttcagggg
     8461 aacattacac ttttataata tgttcaaaga caaacttaac cattcacaaa tataaagaat
     8521 aatattatca aatcattgaa caaatcgtat tttgcaacaa ttgatattta tattaatgta
     8581 ttgtatt
//
LOCUS       DATTQF010000101         8546 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.101, whole genome shotgun sequence.
ACCESSION   DATTQF010000101 DATTQF010000000
VERSION     DATTQF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.101"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            450..1409
                     /locus_tag="VB241_001975"
     CDS             450..1409
                     /locus_tag="VB241_001975"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP1245258.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            1687..2391
                     /gene="budA"
                     /locus_tag="VB241_001976"
     CDS             1687..2391
                     /gene="budA"
                     /locus_tag="VB241_001976"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEP1245259.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2477..3337)
                     /locus_tag="VB241_001977"
     CDS             complement(2477..3337)
                     /locus_tag="VB241_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEP1245260.1"
                     /translation="MLSVAIIGPGAVGTTIVYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3410..4228
                     /gene="panB"
                     /locus_tag="VB241_001978"
     CDS             3410..4228
                     /gene="panB"
                     /locus_tag="VB241_001978"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEP1245261.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEKAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGIEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            4221..5072
                     /locus_tag="VB241_001979"
     CDS             4221..5072
                     /locus_tag="VB241_001979"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEP1245262.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDKVTQYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            5074..5457
                     /locus_tag="VB241_001980"
     CDS             5074..5457
                     /locus_tag="VB241_001980"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEP1245263.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(5586..7268)
                     /locus_tag="VB241_001981"
     CDS             complement(5586..7268)
                     /locus_tag="VB241_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEP1245264.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGITDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGSKYDSQLIIPIVN"
     gene            7325..7433
                     /locus_tag="VB241_001982"
                     /pseudo
     CDS             7325..7433
                     /locus_tag="VB241_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7411..7998
                     /locus_tag="VB241_001983"
     CDS             7411..7998
                     /locus_tag="VB241_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP1245265.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(8079..8528)
                     /locus_tag="VB241_001984"
     CDS             complement(8079..8528)
                     /locus_tag="VB241_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HEP1245266.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEDAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
BASE COUNT         2828 a         1336 c         1656 g         2726 t
ORIGIN      
        1 tatttttaaa atcaatttca tgtttatgtc tgattgccta gtttgtatga aatgtaattc
       61 tttgactata aaaaggtgca tcaaatatga tgagagtaag gcgaaaaata ggaggttata
      121 tagtaagtca tttaagtcga atagccatcc tttttaaaat gaaaaaaata gaaagctcaa
      181 tagtttgtta aagcctttaa atagcgtcat tacgcaattt tagaatgcta aaaattgtca
      241 caaatttcaa attacgtgct aaagcttgag atatcaatat ttattggcgt tagagtgtaa
      301 tttgactccg cttacatctc agttttatgt ttgtaatttg gtagcataat attataataa
      361 aataaaattg ttaatcttta atttcagtat agatattttt acgtgtagtc acgtgtaaaa
      421 taaattcaat taggttagga gacataatta tgaaaacatt tggtaaaaag gttgtattaa
      481 tcggagatgg atctgtagga tcaagctatg cctttgcaat ggttacgcaa ggtgttgctg
      541 atgaatttgt aattattgac attgcaaaag acaaagtaaa agcagatgtt caagatttaa
      601 accatggtac agtccacagt ccttcaccag ttgatgtgaa agcaggtgaa tacgaagact
      661 gtaaagatgc agatttagtt gttattacag ctggtgcacc tcaaaagcca ggtgaaactc
      721 gtttacaatt agttgaaaaa aatactaaga ttatgaagag cattgttaag agtgtaatgg
      781 atagcggctt tgatggatat ttcttaatcg cggcaaaccc tgtagacatt ttaacaagat
      841 ttgtaaaaga atatactgga ttgccagcag agcgtgttat cggttcaggt actgtattgg
      901 acagtgcacg tttacaatat ttaattagcc aagaacttgg tgttgcacct tcaagtgttg
      961 acgctagtat tattggtgag catggtgata ctgaacttgc agtttggtca caagcaaatg
     1021 tagcaggtat ttcagtatat gacacattaa aagaacaaac tggtagcgaa gctaaagcgg
     1081 aagaaattta tgtaaataca cgtgacgctg cttatgaaat tatccaagct aaagggtcaa
     1141 catactatgg tattgcatta gcattgatgc gcatttcaaa agccatttta aataatgaaa
     1201 ataatgtctt aaatgtttct atacaattag atggtcaata tggcggtcac aaaggcgttt
     1261 acttaggtgt accgacattg gttaaccaac atggcgcagt taaaatttat gaaatgccat
     1321 taagtgcaga agaacaagca ttattcgaca aatcagttaa aacattagaa gatactttcg
     1381 attcaattaa atatttatta gaagactaag cctattttaa gtattaatta gaaatatatt
     1441 aatggtaaga ggatctatta gtgttgcggt aacatgtggc actgatagat ctattttttt
     1501 agcaagcatt gatagaggga atacttttgc gtaaaataat aattgaataa agtattatga
     1561 ctcgtgaaaa tttagtgaca tgaatagaaa tgtcgaggcg tcattgagta taaaggaaag
     1621 tagttctgca ttaatcacga agtagagcat gacaacgagg aataactata gggagatggt
     1681 tttggaatga cgaatgtctt gtatcaacat ggtacattag gtacgttaat ggctggctta
     1741 ctagaaggaa cagctacaat taatgaattg ttagaacatg gaaatttagg gattgcaacg
     1801 ttaacagggt ctgatggcga agtaatattt ttagatggga aggcatatca tgctaacgag
     1861 cataaagaat ttatagaatt aaaaggcgat gagaaagtac cgtatgcatc gattactaat
     1921 tttaaagcga gtaagacatt tccattgcaa caattatcac aagatgatgt attcgcacaa
     1981 attaaaaatg aaatgttaag tgagaattta ttttcggctg ttaaaattta tggcacattt
     2041 aaacatatgc atgtacgaat gatgcctgct cagcaaccgc catatacacg tttgattgat
     2101 tcagcacgca gacaacctga ggaaaaaaga caagatattc gtggtgccat tgttggattt
     2161 tttacaccag aattatttca tggcgtaggg tctgctggtt ttcatataca ttttgcggat
     2221 gatgaaagag cttatggtgg acatgttctt gactttgaag tggatgacgt tgtcgttgag
     2281 atacaaaact ttgaaacatt ccagcaacat ttcccggtaa ataacgagac gtttgttaaa
     2341 gcgaaaatag actataaaga tgtggcagaa gaaattagag aagctgaata gtctaatata
     2401 attaaaagac cttagcgata ttaggaacag gtggttctaa atgcatcgtt aaggtctttt
     2461 tatattatat atgtgcttac atattttgtt gatacgcgcg taaaaagcta taaatagtat
     2521 caagatatgg ggtgtcaaga ttgtgttcac gtgcacgtcg ataaataaag ccttgaatcg
     2581 cttcgacttc aagaggttgc tgatgcacaa tgtcgtaata catgctcgtt cccatttcat
     2641 cgggataacc ttgatatata gtcataatag tatcgacagt ttgttctgaa aagtttaacc
     2701 cctctgcctg tgcaacacga caaccatcta gtaatagctg tctacacagt gtacgtattt
     2761 caggattatg catgattgca actgtctgtc taccgagtgc tgtgatagaa ttaatgccca
     2821 agttaactaa caatttatac caaatagctt gttgaatatt tgcttctagt acaatgtcga
     2881 tttgactgtc ttggaccaaa tctctaaatt gtcgagttaa tgcattatct tgtatacgta
     2941 attgataatc tctgaagtgc gtaacaacat cgcctttctt ttgaccactt atatagacaa
     3001 ctgcttggca tacgttttta aatggaatat gttcgagttg accataacca ttttgggcta
     3061 aaatgatgag cgtgtcctca tgtgcaagat gagttaaatg aggaataact gcatcaagtt
     3121 gatgtgtttt gactgcaata ataataacat caaatgtgtt tgtgacatct tcataacctt
     3181 ttacaacaat atcttgtgca ggtgcatgtg gtacagtata atatgttatt gttttggcgt
     3241 gtctcccgat aagcgttgta tgaggcaatg attgttgtaa ttcatagaca atagttgtac
     3301 cgacagcgcc tgggccgata atcgcaactg ataacatctt cttctttcct ttctataaaa
     3361 gattcattat actatttcta ataatactgc aaaaagaggt tgaataacat tgaaaacagt
     3421 aagtcaatta atagatatga aacaaaagca aactaaaatt tctatggtaa cagcttatga
     3481 ttttccaagt gctaaacaag tggaagcagc gggtattgat atgattcttg ttggggattc
     3541 acttggtatg actgtattag gttatgaaag taccgtacaa gtgacgttag cagatatgat
     3601 tcatcacggt cgtgcagtaa gaagaggcgc accaaataca tttgtagttg ttgatatgcc
     3661 gattggtgca gtaggcatct ctatgacaca agacttaaat catgctctaa agctttatca
     3721 ggagacgaat gcaaatgcaa ttaaagcaga aggagcgcat attacaccat ttattgaaaa
     3781 agcaacggca attggtattc ctgttgttgc acatttagga ttaacacctc aaagtgttgg
     3841 cgttatgggg tataaattac aaggtgcaac gaaagaagca gcggagcaac ttattttaga
     3901 tgcgaaaaat gtagaaaaag ctggtgcagt tgcgttagta cttgaagcaa ttccaaatga
     3961 tttagcagag gaaattagca agcacttaac aattccagtc attggtattg gtgcaggaaa
     4021 aggtacagat ggtcaagtat tggtttatca cgatatgtta aattatggta ttgaacataa
     4081 agcgaaattt gtgaagcaat ttgctgattt tagtgttggt gtggatggtt taaagcaata
     4141 cgatcaagaa gttaaatctg gtgcatttcc atcagaagaa tatacgtata aaaagaaaat
     4201 tatgaatgag gttaataaca atgactaagc tgattactac ggtaaaagag atgcaacaca
     4261 ttgttaaagc agccaaacgc tcaggcacaa cgattggttt tatcccaaca atgggtgcct
     4321 tacatgatgg acatttaacg atggtacgcg aatcagttag tacaaatgat attacagttg
     4381 tgagtgtatt tgttaatcca ttacaatttg gtccaaacga agatttcgat gcttatccga
     4441 gacaaattga taaagattta gaattagtat cagaagtagg ggcggacatt gtttttcatc
     4501 ctgcagtaga agatatgtat ccaggtgaat taggtattga tgtcaaagta ggtccattag
     4561 ctgatgtgtt agaaggagcg aagcgtccag ggcattttga tggggtggta acagtagtta
     4621 ataagctatt taatattgtt atgccggatt atgcttattt tggtaaaaaa gatgcccagc
     4681 aattggctat tgtcgagcaa atggtaaaag acttcaatca tgccgttgaa attattggta
     4741 ttgatatcgt tcgagaagca gatggtttgg cgaaaagttc aagaaatgtt tatttaacgg
     4801 agcaagaacg acaagaagcg gtacatttaa gtaaaagttt gctattagca caagcgttgt
     4861 atcaagacgg tgaacgtcaa agtaaagtaa ttatagacaa ggttactcaa tatcttgaat
     4921 cacatataag tggacgcatt gaagaagttg ctgtttatag ttatcctcaa ttagtagaac
     4981 aacacgaaat aactggacgc atatttatct cgttagctgt caaattttca aaagcgcgtt
     5041 taatagataa tataataatt ggagctgaat aaagtgataa gaacaatgat gaatgctaaa
     5101 attcatagag caagagtgac tgagtcaaat ttaaattatg taggtagcat tacgattgat
     5161 tcagatatat tggaagcggt agacatcttg ccaaatgaaa aagtagccat cgtgaataat
     5221 aataatggtg cacgttttga aacatacgta atagctggtg aaagaggtag tggtaaaatt
     5281 tgtttaaacg gtgcagcgtc aagacttgtt gaagtaggcg atgtagtcat aatcatgact
     5341 tatgcacagt tgaatgaaga agaaattaaa aaccatgcac ctaaagtagc agtaatgaat
     5401 gaagacaatg tcattattga aatgattcat gaaaaagaaa atacgatagt gttataaaat
     5461 taaatagcca tcaataataa aaagggccac accttggttt aagattccaa gcgtgtggct
     5521 ttctcttgtt atacagtcct atcactatgt cacttttaat caaaatcgac cgtaattgtt
     5581 gcatatcaat taacgatagg aatgattaat tgtgaatcat atttactacc agtataaatc
     5641 atgtgcatgc ctttatttac ggtttcttca tgttcataac gtgttttcat gccaggggta
     5701 ctattaccaa ttacaatttc actacctttt acaacaactt ccaatgtttc gccttgttta
     5761 aatagcgtgc ctgaaggtaa caattcgatt tcaacaggta caatctcatc ttgtgacaac
     5821 tttaattctg tttcatgttt atgccaaggt tgcgcgattg tggatttttc ttgatctaat
     5881 tcacgatgtg atacgcgtaa ccaaccagta gctacttgac cattttcaat atgattaaaa
     5941 tcagggaagt taacttcatt accacgacga tctaactttt taatacctgc aaataaatcc
     6001 atatcgtctg agtctgtagt gcttacccat agttttaagt tcatgtttcc aactaactca
     6061 gtatctttgt caaacgtata tttaaaactt acatcttgtc gtttatcttc agagtcatat
     6121 tgtgcgacat gcgcgctact gatctttgca tgattcaatg tgtgattttc agcattcaaa
     6181 tacaatggtg tatattctgt gttaggtaaa gggaacgctg acgctgattt gaattcgcct
     6241 ttataaaatt gatctctaac ttcataaatg acatgaggcg tatctttcca atcgttattt
     6301 tcttctttaa ggtaaaaatc aaagaatgat ttttggcgtt cgagattttc tctagcatag
     6361 taactttccc actctttacg tccatgcaca tatagccatt tttcttctga tgctgcttgt
     6421 ttaaatcctt caaaagagcc acggttgtgc aaaccttgtg tagaccaact agcacatgtt
     6481 agtagaggtg ttttaatttg tgataatggc acttgacgct gtttccaaaa atcatcgaac
     6541 aaagggtgtt cttgttgtgc ttgaatcaaa tcttcgatat ttggattatc tgtccatctc
     6601 gcaaaaatac cttgagtcca gaaacgataa aagccagtat ccgttatacc tccgtgaaag
     6661 gctacttctc tatacatatc atttaagcct tcccaaggaa tcattgcttt taaatgtggt
     6721 ggatttaatg atgcgaccca ccattgagtc accgcaagat aagaaacgcc atttgtcccg
     6781 atgtttccat tactccatga ctgatttgct gcccactcaa tcacttcgta ataatcttcc
     6841 gcttctcttt ttgaccatgg agataagacg ccttttgatt tgtcactacc gcgtaatgca
     6901 actttaacta caacataatc atttggcacc caaaatcctg ggtctggtga ttcttcaggt
     6961 gtaaaactag atgtcggaat ggcgcctaat gttggccaaa gggcacccat atttgtgatt
     7021 ttaggcttat tatctttacc gtaagtatct gcagacataa ctacagggaa tttgccatct
     7081 ttatttggtc tgaaaatatt aatatataat ttttcgccat cgcgcatttc cacagtgaca
     7141 tctttttcca taatcatttc ttgatttcca tattgaacac tgtcgacaac gatgtggtta
     7201 ataccggctt tcacttcatt gacatgagtt acagttaatt ttggatttcc tagtaaatgt
     7261 tggttcattg ttatcacctt tttctgtttg tttgtatcac ctatattaaa caggtaaaca
     7321 ttgtgtgcca atcatcaatg ttggagattt gtaggataat gaacataaca tttataattg
     7381 tttataatca ttggggagga cttgatacaa atgactatag atcgacgtat tagaaaatca
     7441 caagttgcaa tgagaaatgc atttattgaa cttttacatc aacaccaatt agaagagatt
     7501 actgtgcaac aaattgcaga tttagctgat gttaaccgta gtacatttta tacgcattat
     7561 tatgataagt atgatttgct tgaaaaatta gaggaccagc agttggagga aataagagac
     7621 ttcattcatg atgagaagtt aaatggagca gttaagttat ccacggataa catacatcaa
     7681 attatgaccg ttttaataga aatgatagaa aaagacatcg cattttatca attgatgttt
     7741 cgtatgggta aagattcaaa tatccacgaa aaactttatg atttaattat gtgtcatttg
     7801 caacgttata aaaatgaaaa tgatcaaatt aatggtatac cattttccta tttcatgagt
     7861 tatgtttcag gtgcgggatt gtctttgata agacattggg tagaagatcc aaaccgtata
     7921 gacaaagttg acttaataag gcattttaat acgattgtca atcaagggcc agcaacgata
     7981 attaaaaata tacaataaaa aaaggcaagc catgaattaa gagtggcttg ccttgctaaa
     8041 taacataagc ttattaaatg atatctttat caataacgtt attgttggat tgcagtatag
     8101 tcttgaccat gtaacatcca acgaacaccg tatttatcgg taaagatgcc cattttgcca
     8161 ccccagaatt ggtcagcaaa cggtaattct atttcaattg aagaatgatc tttaatttgc
     8221 tcatagaatg cttcaacttt atcagcatct tccttattgt taacatcata atcaattaat
     8281 aatgatatgc cattattaat tttgtcagcg cgaccaaaag aatcagaaca tagcactttt
     8341 acaccaagca cttcaaactc agcatgcata gttgcttctt gtgcatcttc cttagtcata
     8401 ccaaaatgtg acgcttgttc ttcgccaact tctaaacgtt taacgtcagt tgcaccaaat
     8461 acttcttcgt aatatgcaag ggcttctttt gaattttcaa aagcgatata agggaataat
     8521 gctgtcatgt aaaacacctc aatgta
//
LOCUS       DATTQF010000102         8532 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.102, whole genome shotgun sequence.
ACCESSION   DATTQF010000102 DATTQF010000000
VERSION     DATTQF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.102"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..447
                     /locus_tag="VB241_001985"
     CDS             <1..447
                     /locus_tag="VB241_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HEP1245267.1"
                     /translation="ATLNESNNWTHTWTGLDEKAKGQQVKYTVEELTKVNGYTTHVDN
                     NDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKPDHSNKVKPTPPDKPSKVDKDDQP
                     KDNKTKPENPLKELPKTGMKIITSWITWVFIGILGLYLILRKRFNS"
     gene            complement(608..1072)
                     /gene="bstA"
                     /locus_tag="VB241_001986"
     CDS             complement(608..1072)
                     /gene="bstA"
                     /locus_tag="VB241_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HEP1245268.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIETILESLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNFK"
     gene            complement(1175..1813)
                     /gene="pcp"
                     /locus_tag="VB241_001987"
     CDS             complement(1175..1813)
                     /gene="pcp"
                     /locus_tag="VB241_001987"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP1245269.1"
                     /translation="MHILVTGFAPFDNQDINPSWEAVTQLENIIGTHTIDKLKLPTSF
                     KKVDTIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVVGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTQ"
     gene            complement(1966..2922)
                     /locus_tag="VB241_001988"
     CDS             complement(1966..2922)
                     /locus_tag="VB241_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP1245270.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEED
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENEAK
                     YLGACSYDCAKHERNRYVQANNISGNEWQQRLTNFDDLHQHA"
     gene            3166..4137
                     /locus_tag="VB241_001989"
     CDS             3166..4137
                     /locus_tag="VB241_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEP1245271.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKRPFVSHKANPAAIKIHKDGRLFICY
                     LGDFKSTGGIFAATENGDNIQDIIEDLSTEYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVTPDFKTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPSRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMDGGSMLYTVNGFAKGHKSYQFQ"
     gene            complement(4283..4798)
                     /locus_tag="VB241_001990"
     CDS             complement(4283..4798)
                     /locus_tag="VB241_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEP1245272.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSITESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGIINREKYGINFNQTLETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5180..6028
                     /locus_tag="VB241_001991"
     CDS             5180..6028
                     /locus_tag="VB241_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEP1245273.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEISEATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            6057..6611
                     /locus_tag="VB241_001992"
     CDS             6057..6611
                     /locus_tag="VB241_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEP1245274.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLIGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNTGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            6664..8376
                     /locus_tag="VB241_001993"
     CDS             6664..8376
                     /locus_tag="VB241_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245275.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNVTGELHINNQDATVSCLHKRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVDMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLREVCMS
                     EHVKFKVKKWIDKTSSNDDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNKGFNQLSISERSEFIGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLIQLFINHDRK
                     VRRQ"
     gene            8373..>8532
                     /locus_tag="VB241_001994"
     CDS             8373..>8532
                     /locus_tag="VB241_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP1245276.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISVSSIL"
BASE COUNT         2932 a         1311 c         1490 g         2799 t
ORIGIN      
        1 gcaacattaa atgaatctaa taactggaca catacgtgga caggattaga tgaaaaagca
       61 aaaggtcaac aagtaaaata cacagtcgag gaattaacaa aagttaatgg ctatacaacg
      121 catgtggata acaatgatat gggcaacttg attgtgacga ataaatatac gccagaaaaa
      181 ccgaataaac caatctatcc tgaaaaacca aaagacaaaa caccaccaac taaacctgat
      241 cattctaata aagttaaacc aactccccca gataagccat caaaagtgga taaggatgat
      301 caacctaaag ataataaaac caaacctgaa aatcctctaa aagaattacc aaaaactggt
      361 atgaagatta taacttcatg gattacatgg gtatttatag gtatattggg attgtattta
      421 attttaagaa aaagatttaa ctcataaacc attataatta tttttataga taaggctatt
      481 cttagttcta tgtataatac atgatattaa taggtcactt ttaatctgta tgtaagcaga
      541 ctaagagtgg ccttttaaaa aataaaaaaa tccgttaacg tgttgttaac ggttaataag
      601 cacatattta tttaaaattt tttaatatac gagacatctc ttcaatttta ccagcatgaa
      661 gtgggatgtg aatggcattt aatactaata actcttctaa gttattacaa ccgacaattg
      721 gttgtttcaa ttcaattgct aaatcatctt cagttagatt tcgtgcacgt tcaggtaatg
      781 tttggagact ttctaaaatc gtttcaatac tcggtacttc atccttccat tcatctggag
      841 acgaaccatt tccgaataaa ggtctatata tatttaaatc aatattctct ttaccagcaa
      901 cagctaaggc tgattcaaaa attgttaata catgcccaaa ttgccaatga atggtattag
      961 aaaaaggctt atgataatca tcaagtacct tttcaacttt ccaagtatca tacacattta
     1021 ctaaatatcc taaacccata tcaatgacat caaatactgt ttttgtagtc attattaatt
     1081 ccgcccccat aaataaaaca tacttccgta agtattaccc tattaacaaa tttgttagtc
     1141 atttaaggaa tgtattttgt ttgtgcattt ataactattg cgttgtgcct agggctaggt
     1201 gtaaatcctc atcattagaa atagcttcaa tagctgcagt caaacctgca actatctttt
     1261 ctaatggcat agatggtgta tcgggtttac caacgacttg ctctggtata tatggcacat
     1321 gaataaaacc gaatcgaagg tgagggtaat gcttatcttg taaataacct aagtgataaa
     1381 gtacgtgatt acatacgaac gtacctgcac tatttgaaag tgctccagga agtccttggt
     1441 taataatact ttgagtcatt gctttaactg gtaaatttga aaaataagct ggcgcaccgt
     1501 ctaaatgaat ggcttgatca ataggttgaa aatcatcatt atctggaata cgtgcatcat
     1561 caatattaat ggcgacacgt tctggggtaa tggcatttct accaccagct tgtcctatag
     1621 ctagtacaac atcataatga ttagatgcca acgttttatt tataatagta tctactttct
     1681 taaaagaggt tggtagtttt aatttatcga ttgtatgtgt gccaataata ttttctagtt
     1741 gagtcacagc ttcccaagaa ggattaatat cttgattgtc aaaaggcgcg aaccctgtta
     1801 ctaaaatgtg cattttaaga caccccgcag tattaaaatt taatttagta taacatgcgc
     1861 ataattaaaa acatatcagg ttatgcatgc gcataaaaaa acagccttga aaaaccttta
     1921 taaatcggat tttcaaagcg tgttttgaaa ggtatttaat taatactaag catgttgatg
     1981 taaatcatca aagtttgtta aacgttgttg ccactcatta ccactaatat tatttgcttg
     2041 aacataacga ttacgctcat gtttagcaca atcataagag catgcaccta aatatttagc
     2101 ttcgttttct tcagaaacta atatttgttt attacattct gggttagcgc aattaatata
     2161 acgttcacat ggtttgccat caaaccaatc cttaccaata atcgtttttt caacttggtt
     2221 gatatcaaca ctgatacgat catcaaatac atacatttta ccgtcccaat attgaccttt
     2281 tgtttcaggg tctttaccat atgtagctat accgccgtga agctgcgcta catcttcgaa
     2341 accttctttt aataaccagc cagaaaattt ttcacatcga atgccaccag tacagtacgt
     2401 aaccactttt ttatctgcaa ataacgcttt attctcttta atccagtcag gcaaatctct
     2461 aaagcgcgtg atatttggac gaattgcacc tcggaaatga cctaaatcaa attcataatc
     2521 attacgtgca tcaataatga ctgtgtcatc atcttcaaga gcttttctaa attctacagg
     2581 tgataaatat tggccagttg tgtgtcttgg atcgacgtct tcttctaaat ctaaagcaac
     2641 aatctctttt cgaggacgta catgcatttt cttaaaagca tgtccttcag cttcatcaat
     2701 tttaaacacc atatctttga atcgttcatc ggcatgcata tgtgccatat attgttcggt
     2761 ttcttctttt gtaccagata atgtgccgtt aataccttct gtagaaacaa gaattctacc
     2821 ttttaaatgg tgtgctttac aaaaggctaa gtgatcctga gcaaactgtt cagggtcatc
     2881 aatcgtcgta tatttataat ataaaagaac ttgatagttc ataggttaat ctccttctaa
     2941 aaacatgtta taagcatgtt aatttttaaa ataaagcgtc aatacgcata tttgtctacg
     3001 tccatcattt taacagagtt gtattgtcat tcaatatttc tagcttgaaa tcagccacta
     3061 gaccgataaa atagataaat gaaaacgtta tcatttgatt tgatagttac cttgtttaaa
     3121 atgaaaatga tagttaaaac gaatgaaatg aaaggaatga tcatgatgtc acaacaagat
     3181 ttacctacat tattttatag cgggaagtct aatagtgctg ttccaattat atctgaaagt
     3241 gaattacaaa caattacagc tgaaccatgg cttgaaattt ccaagaaagg attgcaacta
     3301 gaaggattga actttgatcg gcagggacaa ctctttttat tggatgtatt cgaaggaaat
     3361 atttttaaag taaatccaga aactaaagaa attaagcggc cttttgtaag ccataaagca
     3421 aatcctgcag caatcaaaat acataaagat ggccgattat ttatttgtta tttaggagat
     3481 tttaaatcta caggaggcat ttttgcagcg acagaaaatg gtgacaacat acaagatatc
     3541 attgaagatc tatcaacaga atattgtatt gatgacatgg tatttgattc taaaggtggg
     3601 ttttatttta cagattttag aggatactct accaacccac taggaggcgt ttattatgtt
     3661 acgccagact ttaaaacagt gacgccgatt attcaaaata ttagcgtagc aaatggtatt
     3721 gctttaagta cagatgaaaa agtactatgg gtaacagaaa ctacagccaa tcgattacat
     3781 cgcattgcac ttgaagatga tggtgtgacg atacaaccat ttggagctac cataccgtac
     3841 tattttacag gtcatgaagg accagactca tgttgtatcg atagtgacga taatttatac
     3901 gtagcaatgt atggtcaagg tcgagtgtta gtttttaata aaaggggtta tccaatagga
     3961 caaatattga taccaagccg agatgaaggg catatgttac gttctacgca tccgcaattt
     4021 atacctggca caaatcaact catcatttgt tccaatgata tagaaatgga cggaggatct
     4081 atgctttata cggttaatgg ctttgcaaaa gggcataaaa gttatcagtt tcaataagat
     4141 ctgcctgaat gaagggcgtc ttaatataag tttaaatgtt aaaatagtga atcataaatt
     4201 aaagaaaaat gccaactata cctagggatg atatagttgg catttcgtat atattaaaat
     4261 caatgaggat tatgagatga ggttattctg agattgagaa ttcagctgat gcttcgaatt
     4321 taacatcttt acctagcatt actccaccag tttcaagtgt ttggttaaag ttaatgccat
     4381 atttttctct gttgattata ccagtaacaa taatacctgt tacttgagaa ccatccatag
     4441 gatttttact tacgccgtta aactcaacgt cgaatgtttc ttcgttagtg atacctttaa
     4501 ttgttaaatc acctacaact ttgctttctg taattgattt tgtcacaaaa gtgattttat
     4561 caaattcatc agtaccaaag aaatcaccag attttaagtg gttatcacgt gcttcgtttt
     4621 tagtgttaat tgagcttgga ataattgttg cagtagcttt taaagtgctg aagtcattaa
     4681 tatctccttc aacagcgaca tcaaattgat cgaatgaacc tttcacttta gaaaccatta
     4741 aatgtttaat ttggaactct aaactactgt gtgcaccatc aaaagtaaag tttgtcataa
     4801 tataaaacct ccatattttt atctcttaga tatatttttt atacttgata tacatacgat
     4861 accacttgaa aaacaaagat gcaagcattt tatttcgaat tcgaatattt ttttatgatg
     4921 catataaaag acatatctat aaaacaccac actattgtta taccctagtc aattaattca
     4981 aacataaata agtgtaacca acgttcaata aatttagtta atgagataaa tctgactatt
     5041 attatcattt ttatagtcgc tactattgac tttgtaacaa gtgactcgta ctattttaat
     5101 ataattctga aaattcaaaa tatataacct taaatatgta gataatattt tttgaagttt
     5161 taaaaaaagg aggcatttta tgggaaatta tttggtattg caatcagatt ttggacttag
     5221 tgatggcgct gtaagtgcaa tgtatggagt tgcatatact gtaagtgatg atatacgtgt
     5281 cgaaaactta acgcatgata taccaccgta tgacatttgg gtagcgtcat atcgtttata
     5341 tcaaactgta aaatactggc ctaaaggcac agtttttgta tcagtggtag acccgggtgt
     5401 aggtagtgat aggcggagca ttgcttgctt aacatattca ggtcattaca ttattacacc
     5461 tgataatggc tctttaagtc atatcaaaca ttatgaaggt attaaaaaag ttatcgaaat
     5521 tgatgaagtg aaaagccgat tgcctcattc ggaagaaagt catacttttc atggtagaga
     5581 tgtttatgca tacaatggag cgagattagc tgcaaataag atagcgtttg aacgtctcgg
     5641 tcaggcaatt aatgttgata gtattgaggc attagaaata agtgaagcga caaaaaatga
     5701 ggattctgtt acaggaagta ttgatatttt agatatcaga tttgggtcat tatggacaaa
     5761 tattccattg gcattcttaa aatccttgga aattgttcat ggcaataatt tggtggtaac
     5821 aatttatcat caagataaga aagtctatca aaatattatt aaatttgcgc gatcgtttgc
     5881 agatgttaat gtgggtgaac ctcttgtgta tgttaactcg ctagtaaata ttggtgtagc
     5941 ggtcaatcaa gactcatttt cagatttata tcatattggt actggaaatg attggacgat
     6001 tcatttatct aaagcaccat cgatttaatg aattttttat gtgaggagta atgaagatga
     6061 aaaaacaaga tatttcagtt aaaactgttg ttgcaattgg tataggagca gcggtctttg
     6121 taattttagg gcgttttgtt gtaataccaa cagggtttcc taatacgaat atagaaacat
     6181 cttatgcatt tttagcatta atatctgcaa tatttggacc ttttgctggt ttaatgactg
     6241 gattaattgg tcacgctatt aaagatttca ctacatacgg tagtgcctgg tggagttggg
     6301 ttatttgctc gggaattata ggctgtttat atggatggat tggcctaaaa ttaaatcttt
     6361 cttcaggacg attttctagg aagtcgatga tttattttaa taccggtcaa attattgcga
     6421 atattatttg ctgggcactt attgcaccaa cattagatat tttgatttat aacgaaccag
     6481 ctaacaaggt ttatacacaa ggtgttatct ctgcagtatt aaatattatt tcagttggca
     6541 ttattgggac aatattatta aaagcatatg cttcatctca aataaaaaaa ggtagtttac
     6601 gtaaagaata attattttgt tgaatcagat atgtaaatga atgtagaaag gtaatgatat
     6661 atcatgactg aaccaattat ctcgttcaaa gactttagtt ttcaatatca tagtcaagca
     6721 acacctacat tacagaatat aaatgttgat atttatccgg gagaaaaagt attagtagtt
     6781 ggtgcttcgg gtagtggtaa atcgactttt gcaaattgca taaatggatt aattccattt
     6841 aaaactaaag gtaacgtaac tggggaacta catataaata atcaagatgc aactgttagt
     6901 tgtttacata aaagatctaa tgttgttggt acagttttac aagatacaga tggacagttc
     6961 ataggcttaa cagcagctga agatatggcc tttttattag aaaataattg tgttgaacaa
     7021 gatgatatga agaaaaacgt gagttattgg gctgaaaaag ttgatatgat agaacattta
     7081 aatcaccgac cgcaagattt atctgggggt caaaaacaac gcgtttcatt aggtggtata
     7141 ttaatccatc gtacccctat tttaatattg gatgagccac tggccaattt agatcctgcg
     7201 acaggacatg aaacgctgag attgttaaat aatattcatg aagaaacaaa gtcaacgatg
     7261 attattgtcg aacatcgatt agaagaatca ttagatgata cgtttgatag agttcttctt
     7321 tttaaagatg gaaaaatcat cgctaataca acgcctagtg atttgctaaa atcatctaag
     7381 cttaaagaag caggaatacg tgaaccatta tactgtacag cgcttaaata tgcagaagta
     7441 gatgttgaat ccattgataa cttagcgaat ttacgtgaag tttgtatgag tgaacatgtt
     7501 aaatttaaag tgaaaaaatg gatagataaa acatcttcaa atgatgataa taagtacaaa
     7561 tctgaaccgc tattagaatt aaatgaggtg tgtgttcaat atagtgacta tagtaatagt
     7621 gtacttaata atgttcaatt aaatgtttat agaagagaaa tgcttagtat agttggtcac
     7681 aatggtgccg gcaaatcaac acttgctaaa gcaatttgtg gatttttaga tataacaggt
     7741 aatatccaat tttgtaataa gggatttaat caattgtcaa ttagcgaacg atctgaattc
     7801 ataggttatg tgatgcaaaa tccaaatcat atgatttccg aaaaaatgat ttacgatgaa
     7861 gtagcattag ggttaagagc acgaggtatg aaagaatcag atataaagat acgagtagag
     7921 aatgtgctca aaatatgcgg actttatgca tttcggaatt ggccaattgc agctttaagc
     7981 tatggtcaaa aaaagagggt cactatagca tctgttttag tcttaaatcc ggaaataatc
     8041 atattggatg aaccgactgc tggtcaagat ttctatcatt ataatgagat aatgtcattt
     8101 ttaattgagc taaacagaca ggggaagacg attattatga ttacgcatga tatgcattta
     8161 ttgtctgagt atagttcaag aacagttgta ttatcaaaag gtcaagtcgt tgctgatacc
     8221 acgccagtat tggttttaaa tgataaaaaa atctgtgaga ttgcatcatt gagacaaaca
     8281 tcgctatttg aaatggccga atatataggg attagcgagc cacagaaatt aatacaatta
     8341 tttattaacc atgataggaa ggtgagacgc caatgaatca atataatact ataggttttc
     8401 acccgggaaa tagtcgtatt catcaattaa atgcgactgt taaactttta ttcttattag
     8461 ttgtttctat ttctgcaatg gtgacttatg acacaagata tttaatttta attagtgttt
     8521 catctatttt at
//
LOCUS       DATTQF010000103         8523 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.103, whole genome shotgun sequence.
ACCESSION   DATTQF010000103 DATTQF010000000
VERSION     DATTQF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.103"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            41..173
                     /locus_tag="VB241_001995"
                     /pseudo
     CDS             41..173
                     /locus_tag="VB241_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            435..1094
                     /gene="arlR"
                     /locus_tag="VB241_001996"
     CDS             435..1094
                     /gene="arlR"
                     /locus_tag="VB241_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HEP1245277.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1091..2446
                     /gene="arlS"
                     /locus_tag="VB241_001997"
     CDS             1091..2446
                     /gene="arlS"
                     /locus_tag="VB241_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HEP1245278.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIDYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2920..5718
                     /locus_tag="VB241_001998"
     CDS             2920..5718
                     /locus_tag="VB241_001998"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEP1245279.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGIISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5732..7003
                     /gene="sucB"
                     /locus_tag="VB241_001999"
     CDS             5732..7003
                     /gene="sucB"
                     /locus_tag="VB241_001999"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEP1245280.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNLGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNDETTNNK
                     KEETTNKSADKAEVNQTNDDNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDI
                     DKKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAML
                     TTFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGD
                     DMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMV
                     NGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSY
                     DHRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7593..8402
                     /locus_tag="VB241_002000"
     CDS             7593..8402
                     /locus_tag="VB241_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP1245281.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNCEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSSKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKIGTGGLGGEIHLMPVETEMTMPAYGAVDQV
                     EFETKDVDFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT         3197 a         1253 c         1540 g         2533 t
ORIGIN      
        1 tatattgtta catacttctt ttgaattcat acattaagaa atgttacttg caagttcaaa
       61 caggcacatt gaaatgatgt gtgataaatg tttaaactac ggtcattttg tgaaaataaa
      121 gataataact attcatttta cattaataaa agtcagtata cgacaacaat tgatattgcg
      181 gtaagacctt gtgttacagt attctatatt taagtactgc aatcagaatt aacagaattc
      241 cattaactga ttattaaata tttgagttaa taaataatta atgattgtag cttgaaaaat
      301 ttaaaacatg gttattgatt tgtgataaaa tttaaacgta aacaaactaa tttaaaaagc
      361 aactattgta tagaaaaata caaaatttaa aatatattac cttattagaa aaagtcgtaa
      421 tatgaggtgt acaaatgacg caaattttaa tagtagaaga tgaacaaaac ttagcaagat
      481 ttcttgaatt ggaactcaca catgaaaatt acaatgtgga cacagagtat gatggacaag
      541 acggtttaga taaagcgctt agccattact atgatttaat catattagat ttaatgttgc
      601 cgtcaattaa tggcttagaa atttgtcgca aaattagaca acaacaatct acacctatca
      661 ttataattac agcgaaaagt gatacgtatg acaaagttgc tgggcttgat tacggtgcag
      721 acgattatat agttaagcca tttgatattg aagaactttt agcaagaatt cgtgcgattt
      781 tacgtcgtca gccacaaaaa gatattatcg atgtcaacgg tattacaatt gataagaatg
      841 cttttaaagt gacggtaaat ggcgcagaaa ttgaattaac aaaaacggag tatgatttac
      901 tatatcttct agctgaaaat aaaaaccatg ttatgcaacg ggaacaaatt ttaaatcatg
      961 tatggggtta taatagtgaa gtagaaacaa atgtcgtaga tgtttatata agatatttac
     1021 gaaacaaatt aaaaccatac gatcgtgaca aaatgattga aacagttcgt ggcgttgggt
     1081 atgtgatacg atgacaaaac gtaaattgcg caataactgg attattgtta ccacgatgat
     1141 tacgtttgtc acgatatttt tgttttgttt aattattatc tttttcttga aagatacact
     1201 gcataatagt gagcttgatg atgcagaaag aagctcaagc gatattaata atttatttca
     1261 ttctaagcct gttaaagata tatctgcatt agacttgaat gcatctttag gtaattttca
     1321 agagattatt atttatgatg agcataataa taaattattt gagacatcga atgataacac
     1381 agtgagagtt gaacctggtt atgaacaccg gtattttgac cgcgtaataa aaaaacgtta
     1441 taaaggcatt gattatttaa ttattaaaga accaatcaca acgcaagatt tcaaagggta
     1501 tagtttgtta attcattcac tagaaaatta tgataacatc gtaaaatcat tgtatattat
     1561 tgcgctcgca tttggagtga ttgcaacaat tataactgcc acaattagtt atgtattttc
     1621 aactcaaatt actaaaccgc ttgtcagttt atcaaataaa atgattgaga tccgacgaga
     1681 tggttttcaa aataaattgc aattaaatac aaattatgaa gaaatagata atttagcaaa
     1741 tacgtttaat gagatgatga gccaaattga agaatcattt aatcaacaaa gacaatttgt
     1801 tgaagatgca tcacatgaat tacgaacacc attacaaatt attcaaggtc atttaaattt
     1861 gattcagcga tggggaaaaa aagacccagc agtattagaa gaatcgttaa atatttctat
     1921 tgaagaaatg aaccgtatca taaaattagt cgaagaatta cttgaattga ctaaaggaga
     1981 tgtaaatgac atttcttctg aagcgcagac cgtgcatatt aatgatgaaa ttcgctcgcg
     2041 aatacactca ttaaaacaat tgcatcctga ttatcaattt gatacggatc tgacatctaa
     2101 aaatctagaa attaaaatga aacctcatca atttgaacaa ttatttttaa tctttattga
     2161 taatgcaatt aaatatgatg tgaagaataa gaaaattaaa gttaagacaa ggttaaaaaa
     2221 taagcaaaaa ataattgaaa ttacagatca tggaattggt attccagagg aagatcaaga
     2281 tttcattttt gatcgctttt atcgagtgga taaatctcgt tcaagaagtc aaggtggtaa
     2341 tggactcgga ttatctattg cgcaaaaaat cattcaatta aacggtggat cgattaaaat
     2401 taaaagtgag attaacaaag gaacaacgtt taaaatcata ttttaatcat gtttaagacg
     2461 tcaatcaaag tcatagaatc aattttttaa gtgaggctgg gacataaatc aatgttctat
     2521 gctttacgaa gttatattgg ctgtagttga ctgaacgaaa atgcgcttgt aacaagcttt
     2581 tttcaattct agtcaggggc cccaacaaag agaaattgga ttcccaattt ctacagacaa
     2641 tgcaagttgg ggtgggacga cgaaataaat tttgcgaaaa tatcatttct gtcccactcc
     2701 caagaacgac tataaaacaa ataatcaggg gttctttatc atttctgtgg aattcacctc
     2761 ccaaaattta caataaaata catctataaa cctagaagaa tcaacgcttt tgttgattct
     2821 tctttttagt agataaatag gtaaatctac tttaacaaat aactaaatag tgatattatt
     2881 acattgtaag cgtttcaaca tttttgtgga gggtgtaaaa tgactaacga aagaaaagaa
     2941 gtttcagagg ctcctgtaaa cttcggtgcg aatttaggtc taatgttaga tctatatgat
     3001 gactttttac aagatccatc atctgtacca gaagatttac aagtcttatt cagcacaatt
     3061 aagaatgatg actcaattgt accagcttta aaaagtacaa gtagtcaaaa tagcgacggc
     3121 acaattaagc gtgtcatgcg tttgattgat aatattcgcc aatacgggca tcttaaagcc
     3181 gatatttatc ctgtaaatcc tccgaaaagg aaacatgtac ctaaattaga gattgaagac
     3241 tttgatttag atcaacagac tttggaaggt atatcagcag gaattgtttc agatcacttt
     3301 gccgacattt atgataatgc ttatgaagca attttaagaa tggaaaaacg ttacaaagga
     3361 ccaattgcat ttgagtatac acatattaat aacaataccg aacgtggttg gttaaaaaga
     3421 agaattgaaa cgccatataa agtaacgtta aataataacg aaaaaagggc actatttaaa
     3481 caattagcgt atgttgaagg gtttgaaaaa tatcttcata aaaacttcgt tggtgcaaag
     3541 cgtttttcaa ttgaaggggt agacgcactt gtaccgatgt tacaacgtac tattacgatt
     3601 gctgcgaaag aaggtattaa aaatatacaa ataggcatgg ctcaccgtgg acgtttaaac
     3661 gttttaacgc atgtcttaga aaaaccgtac gaaatgatga tttcagaatt tatgcataca
     3721 gatccaatga aattcttacc tgaagatggt agcttgcagt taactgctgg atggactggt
     3781 gatgtgaaat atcaccttgg tggcattaaa actactgatt catacggtac aatgcagcgt
     3841 attgcactgg ctaacaatcc aagtcacttg gaaattgttg cacctgttgt tgaagggcgt
     3901 acgagagcag cacaagatga tacgcagcgt gctggggctc cgacgactga tcatcataaa
     3961 gcaatgccaa ttattataca cggcgatgct gcttatcctg gtcaaggtat taacttcgaa
     4021 acaatgaact taggaaactt gaaaggctat tctacgggtg gttcattgca tattattact
     4081 aacaatagaa ttggatttac cacagaacca attgatgcac gttcaacaac ttattctaca
     4141 gatgtggcta aaggttatga tgtgccaata ttccatgtca atgcagatga cgttgaagct
     4201 actattgaag caattgatat tgcaatggaa tttagaaaag agtttcataa agacgtcgtt
     4261 attgatttag taggttatcg tcgtttcgga cataacgaaa tggatgaacc atcaattact
     4321 aatccagttc cttatcagaa tattcgcaaa catgactctg ttgaatatgt gtttggtaaa
     4381 aagcttgtta atgaaggtat catttcagaa gatgaaatgc attcatttat agaacaagtc
     4441 caaaaggaac taagacaagc acatgataaa attaataaag ctgataaaat ggataatcca
     4501 gatatggaaa aacctgcaga acttgcattg ccgttacaag cagacgaaca atcatttacg
     4561 tttgatcact tgaaagaaat aaatgatgca ttgttaacat atccggatgg ctttaacatt
     4621 ttgaaaaagt taaacaaagt tcttgagaag cgtcatgagc cgtttaataa agaagatggt
     4681 ttagttgatt gggcacaagc agaacaactt gcatttgcga caatcttaca agatggtaca
     4741 ccgattcgct taactggtca agatagtgaa cgtggtacat tcagtcatag gcatgccgtg
     4801 ttacatgatg agcaaacagg tgaaacatat acacctttac atcatgttcc tgatcaaaaa
     4861 gcgacatttg atatacacaa ttctccgctt tcagaagcag cagtagttgg ttttgaatac
     4921 ggctataatg tggaaaacaa aaaaagcttc aatatttggg aagcgcaata tggtgatttt
     4981 gcaaatatgt cacaaatgat ttttgacaac ttcttattca gttctcgctc aaaatgggga
     5041 gaacgttcag gattaacatt attcttacct catgcatatg agggtcaagg gcctgaacat
     5101 tcatcagcaa gattagagag atttttacaa ttagctgctg aaaataattg cacagttgtc
     5161 aacttatcta gttcaagtaa ttatttccac ttattgcgtg cacaagcagc tagtttagat
     5221 tctgaacaaa tgcgaccatt ggttgttatg tcaccaaaaa gcttactgag aaataaaaca
     5281 gttgcaaaac caattgatga atttacttct ggtggatttg agccaatttt gacagaatca
     5341 tatcaagcgg ataaagttac aaaagttatt ttggcaactg gcaaaatgtt cattgattta
     5401 aaagaagcat tagctaaaaa tccagacgaa tcagtattac tcgttgcgat tgaaagattg
     5461 tatccattcc cagaggaaga gattgaagca ttgctagcac aattaccaaa ccttgaagaa
     5521 gtgtcatggg tacaagaaga acctaaaaat caaggtgcat ggttatatgt ttatccatat
     5581 gttaaagtac tagttgcaga taaatatgat ttaagttatc atggcagaat tcaaagggct
     5641 gctccagctg aaggcgatgg agaaattcat aaacttgttc aaaataaaat tatagaaaat
     5701 gcattaaaaa ataactaggg ggaaataagt catgccagag gttaaagttc cagaattagc
     5761 agaatctatt acagaaggta ccattgcaga atggttgaaa aacctagggg atagcgtaga
     5821 aaaaggtgaa gctattcttg aattagaaac tgataaagtt aatgtcgaag ttgtatctga
     5881 agaagcaggt gtattatctg aacaacttgc aagtgaaggc gacactgtag aagttggaca
     5941 agcaattgct gtcatcggcg aaggtagtgg caatgcttct aaagaaaata gtaacgacaa
     6001 tactccacaa caaaatgatg aaacaacaaa taataaaaaa gaagaaacaa caaataaatc
     6061 ggcagataaa gctgaagtca atcaaacaaa tgatgacaat cagcagcgtg ttaatgctac
     6121 accttctgcg cgtcgatatg ctcgtgaaaa tggtgtgaat cttgctgaag taagtccgaa
     6181 aacaaatgat gttgttcgta aagaagatat tgataagaaa caacaggcac ctgcatcaac
     6241 acaaacaaca caacaagcac ctgcaaaaga agagaaaaaa tacaatcaat atcctacaaa
     6301 accagtgatt cgtgaaaaaa tgtcacgcag aaagaaaaca gctgctaaaa aattattaga
     6361 ggtatctaat aatacagcta tgctaacaac atttaacgaa gttgacatga caaatgttat
     6421 ggaattgcgt aaacgtaaga aagaacaatt tatgaaagat catgatggta ctaaattagg
     6481 atttatgtca ttctttacta aagcttctgt agcagctttg aaaaagtatc cagaagttaa
     6541 tgcagaaatc gacggcgacg acatgattac gaaacaatat tatgatattg gtgtagctgt
     6601 ttcgacagat gatggactat tagtaccatt cgtaagagat tgtgataaaa agaattttgc
     6661 agaaatcgaa gcagaaattg ctaatttagc tgttaaagca cgagagaaaa aacttggctt
     6721 agatgatatg gttaatggtt catttacgat tacaaatggc ggtatttttg gatcaatgat
     6781 gagtacgcca attatcaatg gtaatcaagc tgcaatctta ggcatgcatt caattattac
     6841 aagaccaatt gcgattgatc aagatacaat cgaaaatcgt ccaatgatgt atattgcatt
     6901 aagctatgat catagaatta ttgacggtaa agaagcagtt ggattcttaa aaacaattaa
     6961 agaattaatt gaaaacccag aagacttatt attagaatct taatccacaa cacaaaatag
     7021 tttactattc ctaaaaacgg gatcaaatca ataacaaaca gcagtaagtt tattttctag
     7081 tcgaaatatc ttactgctgt actttatttt tacaatgctt cgtattaaaa tgtgagttta
     7141 attttgacta cttaaaaaat atatcatttc tatatgaaat agactcacga tagaaatata
     7201 gctagaatag cgtgtttcaa tatgctaatg aaatcatttt agataataac aagcgatgaa
     7261 atttatgaat tctataagcg cctttagtta atttaaaatc atctgaacaa gatgtacatt
     7321 cactttatgg acacgacata tatatgtttt tcatgtacta cagaaaaacg aaatatccca
     7381 atatccaatc gctatataca gatactcatt aatgacatac tcaatagcaa ccataacaat
     7441 attatgttag ctatttaaat gaaatagttc ttttaaagaa atacattttt cacatattaa
     7501 tctataatca aaatcaactg accgatattc tataatttat gattaaaata agttataata
     7561 taatagtaaa gataaagata gaggtggcta taatgtgtgg acttagaagt ataacattag
     7621 gtacaacaaa tatagaacag acaaaacatt tcatggttga catattagga ttaaattgtg
     7681 aagaacttct tgaaaactca attcgtttcg gcgatgcaga tataagccca ggaacaagac
     7741 ttcaatttat acaagttcca agtgagcaat tagaagaatc tcactttgtg ggtattggat
     7801 tacgtacacc aactgactca ggtctagagg agtatgcgga aatattatcg agtaaggata
     7861 ttccatttac aacagttaaa gaattaaatg gcaataaata tttcagtctc gaagataaca
     7921 atggtcatat tttctcaata tattcaaacg agaataatta tggcgttggt ttaggtatgc
     7981 cttctttcga aagtgcggtc aatccgttac atcaagtgca aggtttaggg ccagtgattc
     8041 ttaaagtgaa tcatgtagat attacaggtc aaattttaac aaatatattc ggacttgaag
     8101 tatttgcaga ataccaaccc ttcgacaatg ctgactatca tgtccaagta ttcaaaattg
     8161 gaacgggtgg tctaggtggc gaaatacatt taatgcctgt agaaaccgaa atgacaatgc
     8221 cggcatatgg cgcagtcgat caagttgagt ttgaaacgaa agatgtagat ttctttaatc
     8281 aagcgaaatc gcgcttagat gaagtggaaa taccatatca aacacttgag caagatgata
     8341 ttgaatcaat tagaattact gaaaacagtg gattatcgtt tatattcact ttacaaaaat
     8401 aattttttta cgatagtgag gacaaaattt atgttacatg aaacttggaa agaacgtacg
     8461 ccaatcaaga aagtagaagt cattaataca gatgcaaaga aatttacagt ttctgacatg
     8521 tta
//
LOCUS       DATTQF010000104         8518 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.104, whole genome shotgun sequence.
ACCESSION   DATTQF010000104 DATTQF010000000
VERSION     DATTQF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.104"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            52..525
                     /locus_tag="VB241_002001"
     CDS             52..525
                     /locus_tag="VB241_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245282.1"
                     /translation="MEYKSYNKMSNNILSILLSEKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIVGLLTYKVYDEYIEIISLDSFVENKGIGSHLLNYAEIIASDMSKRSISVITT
                     NENIKALYFYQKNKYRITDVIFDAVTEARKIKPSIPEMGENGIEIRDEIVLKKCL"
     gene            complement(632..1618)
                     /locus_tag="VB241_002002"
     CDS             complement(632..1618)
                     /locus_tag="VB241_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEP1245283.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTCKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKLEL"
     gene            1927..2128
                     /locus_tag="VB241_002003"
                     /pseudo
     CDS             1927..2128
                     /locus_tag="VB241_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2177..2473
                     /locus_tag="VB241_002004"
     CDS             2177..2473
                     /locus_tag="VB241_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEP1245284.1"
                     /translation="MATEKDVNDLFLNHVNSNAVKTRKMMGEYIVYYDGVVIGGLYDN
                     RLLVKATKSARHQFQDNALVSPYPGAKEMILIPDFAEATNITNLFECIKNDLKN"
     gene            2681..3283
                     /locus_tag="VB241_002005"
     CDS             2681..3283
                     /locus_tag="VB241_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245285.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNKVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSEFLFLLVIFTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMAYTTIGLSIYLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKKIETVDNTNITER"
     gene            3551..6703
                     /locus_tag="VB241_002006"
     CDS             3551..6703
                     /locus_tag="VB241_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HEP1245286.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAENFKNGAKQAQ
                     LQSFDEYFEIWNDERFIISEEKSEIGEIIFKFYRHCFRYNEINLKIQDIFDDYSDIHN
                     PNGTHCYGYTFNIDKHGQVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDIAYDEYFSVLNSKKDGLFAHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESKRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKTHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESY
                     SKVEHELNSKKMNVKEKLNHLEIQISCDNKEIEDLDDRINYNTKQLETLNELIKSIRD
                     SNKGFVNKLKAIFNSEEDERYKKHNAEKQQLLGQQIELEKCKKIKNEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKSRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALFRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYNVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITNVGKTGWYDVKGNSVQKQFVKEHGEK
                     VLELLANDWFEAIKEGKNEPSSFVISPFSAVQKQVKRILKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQSKPFYETIFKERNVK"
     gene            6786..7268
                     /locus_tag="VB241_002007"
     CDS             6786..7268
                     /locus_tag="VB241_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245287.1"
                     /translation="MKKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIESLIGLRFLKEYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
     gene            7473..8459
                     /locus_tag="VB241_002008"
     CDS             7473..8459
                     /locus_tag="VB241_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HEP1245288.1"
                     /translation="MKKCIKTLFLSIILVVMGGWYHSAHALDSLSKSPENWMSKLDES
                     KHLTEINMPGSHDSGSFTLTDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLQVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
BASE COUNT         3120 a         1133 c         1511 g         2754 t
ORIGIN      
        1 aatcatcatt ttcaactaat aaggattatt aacagagaga agtgacttat tttggaatat
       61 aaatcttaca acaaaatgag taataatatt ttatctatct tgttgagtga aaaaaatttc
      121 gataataaaa tggtagtctc aacaggtagt tacattttgt ctgagttgcc atgtattgtt
      181 gcctataaaa ataatgatat cgtggggcta ctaacgtata aagtttatga tgagtatatt
      241 gaaatcattt ctttagatag ttttgtagag aacaagggta ttggtagtca tttacttaat
      301 tatgctgaaa taattgcctc agatatgagt aaaagaagta tcagtgttat tactacaaat
      361 gaaaatatta aggcactcta tttctatcaa aaaaataaat atagaatcac agacgttatt
      421 tttgatgctg taacagaagc taggaaaata aagccgtcga ttcctgaaat gggtgaaaat
      481 ggcattgaaa ttagagatga aattgtattg aaaaaatgtt tataacgtat aaatatacgt
      541 tgttttaaat caccctagtt cataccaaaa agactttaac acaaacaaaa aggaggaaac
      601 ttaaattcct cctcaacctt tattactcat actataattc tagtttaacg tcttcgtcca
      661 tttgggcttc aaattcatct agtagtgctc gtgcttctgc aattgattgt gtgttcatca
      721 attgatggcg aagttcacta gcgcctctta tgccacgcac atagatttta aagaatctac
      781 gtaaactctt gaattgtcgt atttcatctt tttcatattt gttaaataat gatagatgca
      841 atctcaacag atctaatagt tctttgcatg tgtgttcgcg tggttctttt tcaaaagcga
      901 atggattatg gaaaatgcct ctaccaatca tgacgccatc aatgccatat ttttctgcaa
      961 gttcaagtcc tgtttttcta tcgggaatat caccgttaat tgttaacaat gtgtttggtg
     1021 caatttcgtc acgtaaattt ttaatagctt cgattaattc ccaatgtgca tctactttac
     1081 tcatttcttt acgtgtacga agatgaatag ataaatttgc aatgtcttgt tcgaagacat
     1141 gcttcaacca atctttccat tcatcgattt catagtaacc aaggcgtgtt ttaacactta
     1201 ccggaagtcc acctgcttta gttgcttgaa taatttcggc agcgacgtca ggtcttaaga
     1261 ttaagccgga acccttaccc tttttagcaa catttgctac aggacatccc atatttaagt
     1321 caatgccttt aaagcccatt ttagctaatt gaatactcgt ttcacggaac tgttctggct
     1381 tatctcccca tatgtgagcg accatcggct gttcatcttc gctaaaagtt aagcgtccgc
     1441 gcacactatg tatgccttca gggtggcaaa agctctcggt atttgtaaat tcagtgaaaa
     1501 acacatccgg tctagctgct tcacttacaa cgtgtcgaaa gacgatatct gtaacgtctt
     1561 ccattggcgc caaaataaaa aatggacgtg gtaattcact ccaaaaattt tccttcataa
     1621 tatatttata ccctctttat aattagtatc tcgatttttt atgcatgatg atattaccac
     1681 aaaagacgaa cttatacaaa aggaatttta gttgatacaa ccatttgaaa gggaagtcta
     1741 cgagtagtct aaaatgaatg ttgtggtaag ttgatcaata tacaaatcaa ggattatcgc
     1801 gttaaattgt tcattattaa tgatacacta cttattatta tgattcagaa ttttctttgg
     1861 ctacatatac agtaatgtga tttatacgtt atcttataac aaaggcaaat ttataaaggt
     1921 gatattatgg aagatttaaa gcaatcttta aaaggtttag gttggtatga ctttttttat
     1981 agtacctatg tttctactat tcgtgtatct gccgaattat aattttataa ctatatttat
     2041 taacattgtt atcattattt tcttttccat aggtttgatt ttaactacgc atataattat
     2101 agataaaatt aagagcaact cgaaatgatt cattaacacg aatgtgatta aacataaaac
     2161 tgaaggagcg attacaatgg cgactgagaa agatgtaaat gatttatttt taaatcatgt
     2221 gaattcaaat gctgttaaaa ctagaaagat gatgggagaa tatattgttt attatgatgg
     2281 tgtggttata ggtggtttgt atgataatag attattggtc aaggcgacta aaagtgcacg
     2341 tcatcaattt caagataatg cattagtatc gccgtatcct ggtgctaaag aaatgatatt
     2401 aattccagac tttgccgaag caacaaatat tactaattta tttgagtgca taaaaaatga
     2461 tttgaaaaac taatcataaa ttgccatgct caactatttt gaaaagcatc atttccatat
     2521 tatcaaatta ttgagtatct cgatttattg ttatacacaa acagtatttt tgttatcata
     2581 gagcttataa atataataaa ttgaataagt aactttacaa aactagaatg attcaattca
     2641 atcacaatta tttgaaaata gaggtgagca ggtgaacgat atgttaatta gtctcgtaat
     2701 cccagttttt gctttgttag ttattggtgg tattatttgg atgattatag aaggtatagt
     2761 acatatttca aaaaagaata aagcaattga taactttttt aacaaagtta ataaagtaag
     2821 tgagacatat aaattcgcta ctactttttt atttctaatc ttggctacgg ctggtatttc
     2881 tcaattttat ctatattata tagtatcaga gtttcttttt ttgctggtta tatttacctt
     2941 tggtattgca ggtatcattt ttttaatgcc atatggctta tgtttcctac cactttataa
     3001 acaaaaaaag aaaaaacaga catttaaaaa atacatggct tacactacga ttggtttgtc
     3061 aatttatcta ggcttatctc tagttttggt tcacactacg aaaatttata tggacgaagg
     3121 tggcgtaaga tactattacg gtagttttgt aatgaaacaa gcgggcggtt atgcttattt
     3181 agctttagca gtactttcaa cgttgttaat tgttgcgaaa aaagctacaa ataaaaataa
     3241 aaaaatcgaa accgtcgaca atacaaatat aacggaaaga taattaagag agtgctcatt
     3301 caggagtgct ctttttttga tgttcaaatt tagttggaaa tgaaggcata gaagaaattt
     3361 ttatagtcat ttattggata atgaattatg gtcatatcga ttaattttat tagtgaggat
     3421 tttacaaaca tttgttgaat aaatatggta atgataatat atgaatattt ttgaaaaaac
     3481 actaatatga ttaaattatt tgttaaaata gcattaagta aaaaacaaac agggaacaag
     3541 gtgggatttc atgagtcaat tgctaaatga tacgttatcg gcttggttgt taattgaatc
     3601 tttaagtcca ggagaagtaa attttacagc ggaagatata ctctcagctg aaaattttaa
     3661 aaatggtgca aagcaagcgc aacttcaaag ttttgatgaa tattttgaaa tatggaatga
     3721 tgaacgcttt attatatcag aagaaaaatc agagattggc gaaatcattt ttaaatttta
     3781 cagacactgt ttccgctata atgaaattaa tttgaaaatt caggatattt ttgatgatta
     3841 ttcagatatt cataatccaa atgggacaca ctgttatggt tacacattta atatagataa
     3901 acacggtcaa gtgatagttg attctataca tattccgatg attatgagtg cattgaaaga
     3961 aattgaaaag aataaaaatg ctaatataga agaaaaattt aatgattctg ttgaaaaatt
     4021 tgttcaaaaa gtaaaagaaa ttttagcgga tgaaccaatt aatgaattta aattgaagaa
     4081 gatggacata gcttatgatg agtatttttc tgtattaaat tcaaagaaag atggattatt
     4141 tgcacattat gtagcaatag aatatgtgaa agatagtgat ttaccacagc cagaatttaa
     4201 tagcttcttc ataagtgata ttgagaaagc aagaaaatct cccaatcaaa ctttaattga
     4261 ttacattgag ggtgtagaag aaagtaagcg catagaagtg gatgaaaata aagaaatgtt
     4321 cgacaaattt ttacatcctt cacgtttgcc tgatggacgc tggccatcac agactgagtt
     4381 tagattgtca ttaatgcaac aacttgctgt aaaccaaatt acgagtggta atgaaaggat
     4441 aagttcagtt aatgggccac cagggacagg taagactact ttattaaaag atatatttgc
     4501 gcatctagta gttgaaagag gtaaagagtt agccaaacta aataatccta aagatgcatt
     4561 tgtcaaaaca aaaactcatg aaacagatga taaatatgtc tatttactta aggaatctat
     4621 tgctaaatat aagatggtag tcgcatctag taataatgga gctgttgaaa atatatctaa
     4681 agatttaccg aaaattgaag aaattataag aaatcccgaa aaatgtaaat tccctaaata
     4741 tgaacagaat tatgcaaatt tagcacatga attaaaagat tttgctgaaa tagctgaaga
     4801 tttgattggt gaaagtgcct ggggcttatt ttctggagta tttggtaaaa gtactaatat
     4861 taaccaagta ttgagtcata tgttaaaaca agatgcgaat gatattggct ttgctaaatt
     4921 actacaaaat gagaataatc gtatgagtta taacgagtta atgagtgaat ggcaatcaca
     4981 tcaacgtgca tttttagaag agttgaggca tgttgaaatg ttaaaagaag aatctattag
     5041 agcctatgat gtttataaaa attgtgagtc ttactctaag gttgaacatg aactaaatag
     5101 caaaaaaatg aatgttaagg aaaagttgaa tcatttagaa attcaaatat cttgtgacaa
     5161 taaagaaatt gaagatttgg atgatcgaat taattataat acaaagcaac tcgaaacttt
     5221 aaatgagtta attaaatcca tcagagatag taacaaaggg tttgttaaca agctgaaggc
     5281 gatatttaat tcagaagaag atgaaagata taaaaaacat aatgcagaga agcaacaatt
     5341 attaggacaa cagatagagt tagagaaatg taaaaaaatt aaaaatgaag accttgttag
     5401 caaactaaaa gaaaaagaga aattaattaa acaattaact aaagtacagt tgcaattaga
     5461 cgagttaaat tcacagttac aagagttaga agcatatcgt attgagtcaa aaattacaat
     5521 tccagaaaaa gatttttgga gtgacaacaa ttatgatgag cgccaagtta ctaatttgtg
     5581 gacgagtgac gaacttcaat acagacgtgc catgctcttt ttaagagcaa tgatattgca
     5641 taaattatta ttgattgcta ataatacaac tatttattat gcgattaatg attttaaaag
     5701 tagaaggaaa ttaattgatg caaatccaga taaagtacac aacgcatgga atgtgatgca
     5761 tttaatattt ccagtagtta gtacgacgtt tgcaagcttt aaatctatgt atgggggcat
     5821 accaaaagat ttcatagact acttatttat tgatgaagca ggacaagcaa tacctcaagc
     5881 agctgtggga gcattattta gatcgaaaaa agttgtagcc gtaggtgatc cgattcaaat
     5941 tgaacctgtt gttactttag aaagccattt aattgataat attcgtaaaa attataatgt
     6001 tccggaatat ctagtttcta aagaagcttc tgtgcagtct gtcgctgaca acgctaatca
     6061 atatggcttt tggaaatctg atgctactga tagtaatcaa aaaacgtgga taggcatacc
     6121 tttatgggtg cacagacgat gtttaaaacc catgttcacg atagctaatc aaattgctta
     6181 taataataaa atggtgctac ctagtaatat tacaaacgta ggtaagaccg gttggtatga
     6241 cgttaaagga aactcagtac aaaaacaatt tgtgaaagag catggcgaaa aagtattgga
     6301 attactagct aatgattggt ttgaagcgat taaggaaggt aaaaatgagc cgagttcatt
     6361 tgtaatatca ccattttcag cagtacaaaa acaggttaaa cgtatattaa agcaacaact
     6421 accgactaga attgatattg aacgtacaaa aattaatcaa tgggtcgata aatccattgg
     6481 tactgttcat acttttcaag gtaaagaggc tcaaaaggtg tattttgtaa taggtactga
     6541 taatactcaa gatggtgctg tgaactggtc atgcgaaaaa ccaaacttgt taaatgttgc
     6601 agtgacaagg gctaaaaaag agttttatgt aattggtgac atgcaaagaa tacagtcgaa
     6661 accattttat gagaccattt ttaaagaaag aaatgtaaaa tgaaatgaca gcatttctat
     6721 aggctaatca agtgatactt aattaccgcg caatttatac attttaaaag gagtaaaatt
     6781 gaataatgaa gaaatttaac aactggatat taaatgcaat aagtggatct caaacagaca
     6841 agaatgaaac aactgaagaa ttaaaagggg caaaatttat tattttatat gcatattcaa
     6901 tgctcgtttt gcttgcgtta gtaatttcta acatattcat tcacattttg gagcctaaac
     6961 tatcaatcac cactcaaatc atcatcgttt tgattttaat tgaatcacta attggactgc
     7021 gtttcttgaa agagtacgat gttaagcgtg gcaaagataa agaaaataag aaaaatagta
     7081 aggatttcgt taaactaaaa tcaattttag tagcaatttt atttacatca ttggcgctga
     7141 cagcaggcac tgtagctgat atatacggtt tcactgactt aggaaatact agaagtgatt
     7201 taatcgtttg gagcataggt ggtattatat ttggcctcgt atgttacaca atggaagata
     7261 aaaaataacg ataaggagct ggcgattata aagctagctc cttttttaac ttatatatgt
     7321 aaagaactat tctaaggatt tttaatcata tgtcaataat tcctataata cattattaaa
     7381 acatcaatta aataagcttt aaaattttac acatattttt atttaaaaaa gatgtataat
     7441 taatgtatta aatatagaaa gaagttgata ttatgaaaaa gtgtattaag actttgtttt
     7501 taagtatcat tttagtagtg atgggtggtt ggtatcattc agcacatgcg ttagattcgt
     7561 tgagtaaaag tccagaaaat tggatgagta aacttgatga aagcaaacat ttaactgaga
     7621 ttaatatgcc aggctcacat gatagtggct cattcacttt aactgacccg gtaaaatcag
     7681 tttgggcaaa gactcaagat aaagattacc ttactcaaat gaagtcagga gtcaggtttt
     7741 ttgatattag aggtagagca agtgctgata atatgatttc agttcatcac ggcatggttt
     7801 atctgcatca tgaattagga aaatttctcg atgatgctaa atattatttg agtgcctatc
     7861 caaacgaaac aattgtgatg tctatgaaaa aggactacga tagcgattct aaagttacga
     7921 agacatttga agagattttt agagaatatt attataataa cccgcaatat cagaatcttt
     7981 tttacacagg aagtaacgcg aaccctactt taaaagagac aaaaggtaaa attgtcctat
     8041 tcaatagaat gggtggtaca tacataaaaa gtggttatgg tgctgacacg tctggcattc
     8101 aatgggcaga taacgcgaca tttgaaacga aaattaataa tggtagctta aatttacaag
     8161 tacaagatga gtataaagat tactatgata aaaaagttga agctgttaaa aatttattgg
     8221 ctaaagcaaa aacggatagt aacaaagaca atgtatatgt gaatttctta agtgtagcgt
     8281 ctggaggcag cgcatttaat agtacttata actatgcatc acatataaat cctgaaattg
     8341 caaaaacgat taaagcaaat gggaaagcta gaacgggttg gctgattgtt gactatgcag
     8401 ggtatccgtg gcctggatat gatgatatcg taagcgaaat tatagatagt aataaataag
     8461 gattcaataa tgatattaag acgagtatga aaatagttag attctaatta ttttcact
//
LOCUS       DATTQF010000105         8283 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.105, whole genome shotgun sequence.
ACCESSION   DATTQF010000105 DATTQF010000000
VERSION     DATTQF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.105"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            229..1101
                     /locus_tag="VB241_002009"
     CDS             229..1101
                     /locus_tag="VB241_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP1245289.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1484..2863)
                     /locus_tag="VB241_002010"
     CDS             complement(1484..2863)
                     /locus_tag="VB241_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1245290.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3143..3499
                     /locus_tag="VB241_002011"
     CDS             3143..3499
                     /locus_tag="VB241_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245291.1"
                     /translation="MLKQILPRATKISVIFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFSIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3496..3681
                     /locus_tag="VB241_002012"
     CDS             3496..3681
                     /locus_tag="VB241_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245292.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3777..3968)
                     /locus_tag="VB241_002013"
     CDS             complement(3777..3968)
                     /locus_tag="VB241_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245293.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILAIIGYFLLLQGKL"
     gene            complement(3979..4614)
                     /locus_tag="VB241_002014"
     CDS             complement(3979..4614)
                     /locus_tag="VB241_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1245294.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDEYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4689..5606)
                     /locus_tag="VB241_002015"
                     /pseudo
     CDS             complement(4689..5606)
                     /locus_tag="VB241_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            5874..6404
                     /locus_tag="VB241_002016"
     CDS             5874..6404
                     /locus_tag="VB241_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245295.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6470..8074)
                     /locus_tag="VB241_002017"
     CDS             complement(6470..8074)
                     /locus_tag="VB241_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP1245296.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSSKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVVIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDNVDPKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVEDTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
BASE COUNT         2926 a         1547 c         1138 g         2672 t
ORIGIN      
        1 cttacggtaa tatatcagta tattgatata ttatttgcac atgggagtat gctatttttt
       61 atctatacat cttaaaaaaa attacagttt tataattcaa tatcattaaa caaacaacat
      121 attcagctta tttttcattc tcttacgtta caagttgtaa ataccatcac gttaaacaac
      181 aatagccaac gctaataaaa tatattgcgt taaagtggag tgattaatat gtcaaacgta
      241 ctaacagaaa tagatagtca atatccatat atgactaaaa atgaaaaaaa gatagctaaa
      301 tttatactaa attcacctca aaaggtgatt aaaatgaggt ctcaagactt agcaagctta
      361 ttagatatta gtacatcatc agtcattcga ttcagcaaga aaattactga tggcggtttt
      421 cacgacttga aaataaatat atctaaatat gttcctaaag catcatcaat ttacaatgtt
      481 gaattaatga ataacgaaag cacagaatct ttaagaacta aactccatac acgtactaca
      541 cgcgcactta atcatgcaaa taacgaatta aatgataaaa ctattgatca aatatgtcat
      601 tgtttaaaac gttctgagac gatttttata tatggttttg gcgcatcttt tgtagttgct
      661 accgacttat accaaaagtt atcaagaata ggtcttaata ttcaacttgt tcaagaaaca
      721 catatttttg caacattatt agcaactcac aattcgaacg atagtgttat tcttattact
      781 aacaatggta cacaaagtga aatgcaatca atggttaaag ttattgatga ctaccatata
      841 cctataatca caattactag tacaagggat aatcccgtag cacaggcatc aaatattgtt
      901 ttaacatatg ggaaaactga tgaaaatgag atgcatatgg gagcaacaac ttcattattt
      961 gctcaaatgt tcactattga tattttatat tatcgttatg ttgcattaaa ttatcacgca
     1021 tcattagatt tcataacaca atctaaaatg gctttagata actatcgtaa acatttatca
     1081 aatattaact ttaaacatta acagtcttat ataaattctt taatcatgtc atataataat
     1141 ttatgcatca ttttcaaagc ttacatgtct tttatcttgt ataacatacg catatgacca
     1201 atgatttgat aaggccattc tatttcttat attaaaaaca aacgcagttg attaacggat
     1261 tcattccata tatcaattgc gtttgttatt ttaataatgc tattttttta agcccacagt
     1321 aaaaactaac gatagtattg ccacttataa gtaaattcat actacatctt tgttttaagc
     1381 tatatttata aaaaatgcgc ctactcataa catttctttg gctatttaac tttaataagc
     1441 tagggccaag tttgtcataa aataagcgca cattattatc atactatttt gcttttacat
     1501 tatttttatt atatctaata aaataataaa tcgttacaat cgttaagaag atgacaccaa
     1561 taagcactga tattctagtt tcaacattaa ataccatacc gaccaatact aatattaaga
     1621 ataatattgt taagtagtta cttactgcgc caccaggcat tttgaatggg tgaccttcca
     1681 cttcttctgg gtgtaatttt ctaaaacgta agtgactgaa taaaatcata aaccaaggaa
     1741 tcatacctgg taaaatagac gcactatata cgtatacgaa aatactcttc gctccatcga
     1801 tatataaagg taaaattaca tttaataaag caccaattaa aataccaaga gatactgcaa
     1861 caacagtgta caatggcaca ccatttctca tgattttagt aaatattttc ggcatttcac
     1921 ctttatgagc aagtgtataa atcatacgac tcgcactgaa aatacctgag ttacatcctg
     1981 acattgctgc agttaataca acaaagttaa ttaatccagc tgcaaatgta ataccgattt
     2041 tagcaaatgt tgcaacaaac ggacttccaa tgtctcctaa ttgattccaa ggatacacag
     2101 aaacaataac aaaaatagca cctaaataga aaattaaaat tctccagatt acaccattca
     2161 ctgctttaac gatatttttc tgaggatctt tagtttcacc tgcagtaata cctatcagtt
     2221 ccacaccttg gtatgaacca attacaattg ataatgcaaa gaagaagcca acaataccat
     2281 ttggcataaa tccgccattt gtccataaat tagaaatacc taccgcatgg ccaccatttc
     2341 caaaaccaaa gaaaataaga ccaaaaccag caataatcat taaaataatt gttacaactt
     2401 taattaaagc aaaccaaaat tcaaattctc caaacgcttt taccgagaat aaattcgctg
     2461 ccattaataa taaaatagca ataacaccag gaatccaagt tggcaattcc gggaaccaaa
     2521 acttcatata ttctcctact gcgatgacct cactcatacc aacaacaatc cattggaata
     2581 tattactcca tgctgtcata taacctgctg caggatgtat ataatcactt gcaaatgttg
     2641 caaatgatcc tgttgtaggg tttaaataaa tcatttcccc cattgctctc atgattaaaa
     2701 ataaaaagat acccgcaatt aaatatgcaa ggataactga tgggcctgtc catttaattg
     2761 tacttgtggc acccatgaaa agccccacgc caatcgtacc accaagtgca atcatttgaa
     2821 tttgacgggc actcaatccc ctttgtagtt cattgttctc cataacttct taccccatct
     2881 ctgattgata ctaaataaat ccataaaaaa acttattgcc gtaattatac tataaattat
     2941 cgattttggt acattaatat ttgcatacca tgcaatcgtt tacagaacta ctatagcagg
     3001 cttttgcaac tttgcaaaca attattttgt gatttttcta aaaaattaaa aaagttgtcc
     3061 atgaaccgtt gcgtcacata caacttcagc tattctcggt aattatgata taattttaat
     3121 aatattaaaa aggagtatat caatgttaaa acaaatactg ccacgtgcta caaaaatatc
     3181 ggtaatcttt gctgtagcat ttttcatcat aaattatatc ggtatggaaa aacctgacat
     3241 cctatattta gttggaagaa ctattattgc aacacttgct ttcatactca tttgtcttac
     3301 agtattttct attattaatt ctccagagcg caagattaaa ctcggtacga ctttaccgat
     3361 tgcattaatt attggtatca ttttcggcgc catattttta acggtacaaa tcggtgtcat
     3421 cactgggtta atcatcggtg tgattgctac gtttatttgg gaattgattg aaaaaaataa
     3481 aggaggtcgt tcatcatgac aacagcatta attggtgtaa ttgtattaat cttactaatt
     3541 attagctttg tgccaaacta ccgtgctatg aaatcagcaa aagaacaagg aagtaatgca
     3601 acaagagcta caattatggt aggcatagat attatcttaa ttgtacttat catcgtgacg
     3661 ttattattaa aatatatgta actatttaaa ccaccctatc tcatacataa acatgaacgt
     3721 tctaccattt cacatacata tggtcgtcca tttaatgagg taaggtggtt ttctcgttaa
     3781 agtttacctt gtaatagtaa aaaatagcca attattgcga gtattaagta aatcattgaa
     3841 ccaacaacaa aagatcttac ttgcacgcga aatggtgtct gtttgttaaa tataggacca
     3901 atcaccaaat ttccaacgcc taaaattact ggaccagcta gaaaaaacaa tataatgtaa
     3961 cccatcatga tacgcctcct atttcgtaaa gaaacgcgct acaatttgat cgtaatcaat
     4021 atcttttgct acataatcta cattagaaaa gtcctgtgcg cttgtcattt tattcgtatt
     4081 aacaataaca tctaatccag ctgcaatcgc ggctgttgca ccatttacag aatcttcaat
     4141 agccaaacat tgtgtaggca tataatttaa ttgttgtact gcagataaat ataattcagg
     4201 gtcaggttta acttgttcaa catcttctct accaacaact acctctatat actcgtctag
     4261 acctaatgct ttaaaggtag gcattatttc cgctttcaca ctacttgtgg caatcgccat
     4321 aggaatgtga cgttgcttta aaaatgccat caatgattta atcgtatcaa tcatcggtaa
     4381 tgttgtacta gtaagatgat gttcttcata aagtttatct ttattttccg aaccaatcgc
     4441 tttaattaaa tggtcatgca aatctgtagc tgctcctcca atagaagaac gataaaaatc
     4501 aatgcttata ggatcggcat tatgcatctc taaatgttta ttaataacat taaataaatg
     4561 ttgttccgta tctattattg ttccatcgaa atcaaatata actgctctat acatatattt
     4621 actcctctat tttttctctt tatcaaagct taacacagtc cgatgaccac ttcattctat
     4681 tagtgcaatt ataacttttt tgaccaatac tttgctaaaa taggccctgt tatattatga
     4741 atgaaactaa acactgcgcc tggtactgct gcaattggat taaaatgcaa tgctgctaat
     4801 gacacagcta aaccagagtt ctgcattcca acttcaatac tgactgcttt ttgatcttgt
     4861 cgatctaatt ttaaaagacg agctaaccaa tatccaatcg tataccctaa tacgttatgt
     4921 aacataacta ctaaaaatat taacaatcct gtagttaaga tttggtgctt actgccacct
     4981 acaactattg ctaaaattaa tgaaatagca acaactgata taatcggcaa agctgtagaa
     5041 gctttttcag caattttacg attaataatt tacaaaacaa tacctaaagc aattggaatt
     5101 aatacaactt gaacaactga ccacaacata ctcaggaaag atacttccaa ccattcattt
     5161 gcaaatagat atattaacgc aggtgtaacg aatggcgcta gcaacgtaga gaccgtcgta
     5221 atagaaacag aaagcgctac gttagctttg gctaaataac tcattacatt acttgatgtc
     5281 ccacccggac aacatccaac taatattacg ccaacagcaa tatcaggtgg taaatgaaaa
     5341 gactttgcaa ttataaatgc taatgtgggc ataatactga attgtaggca gacaccaata
     5401 attactgctc taggtgcttt aaagaccatt ttgaaatcat taggtgtaat tgttaatccc
     5461 atacctaaca taacaatacc aagtaaataa ggtacccact tacctaatgt cgcaacatgt
     5521 tgtgggaaaa taaatccaat aacagctgct actaacatcc ataccaaaaa actcttcgtg
     5581 gcaaatatac tcaatttttg aaacattgtt aacacatcct cgtcgataat attgaaaaat
     5641 attaccataa gaccagtaag gttacaatta cttttctgaa aattgagatt atttagccat
     5701 tctcttttta ttgcaaatat tttatcactg ataaacaatt attttaaact tcattcaaaa
     5761 gtcttagtga ataaagaaaa aaagcgtggt aaaatcaact tgaacaaaat taagtcacct
     5821 ttataaatat taaacgtgta tttaatgcca tgtattcctc gggaggtcct cacatgttaa
     5881 ttgataaatt cgaaacatac atcatcaaca tcgccggttt aaatgaccgt actactagaa
     5941 aaaaattaag taagttatgc aaaagtgtac aattttgtga tgcgttgcaa ttctcaataa
     6001 acaaacagtt taatcagtat gtattagaaa ttagcttacc aaagcaacaa cttccatact
     6061 tcatttcatt tttaagtttt catcagtata gtattttcca agtactttca ccaaagaaaa
     6121 taaatgaatt acttgattct gataatttat atcaatctgc aaagcgtttc gatatcaata
     6181 ttgatggctt gcaagatgct ttcattaaag acaaagtcat cgatattatg aatatgttcc
     6241 aaaatcacac tgatatcact tatacgttga ataagtctca tgcgcatatt atatgtacgc
     6301 cagaaatatt cgctaaattg ttacatacga ttgcaactag aaatatcgac attctatctg
     6361 ccaattatag atcgtcatct atgagcaaag ctcgtatatc ataataaaag acacatctat
     6421 acgatgatca tttacaatga ttgcgtatag tgatgtgtct ttttttgtat tatttatctt
     6481 cggacgattg atcctcaacc atttgttcaa atgcttcacg aacttgtggt actgtcatac
     6541 ctactacaac ttggatactc ttaccactct ttactaatcc gtgtgccatt tgttgatgcg
     6601 tgaaatattc cgtatcctca actttacttt catcataaac agttaagcgt aatcttgttg
     6661 tacagttagt aacatctttg atattttctt tgccgcctaa accgtctagg taatacgcag
     6721 ctttgtcttc gtattcatta ccagttgaag cacgtttagg gtcaacgtta tcaccttttt
     6781 tatttttata gtcttgttta gagaataact taacctcttc ttctgttttc ttacgtccag
     6841 gtaatggaat atcaaatttc aaaattaaga atctgaataa gaagaagtaa atcgctacaa
     6901 atattaagcc tataacaact tgtgctacat aagtgcccca atgttctttc ccaagtggta
     6961 accagtttgt tgaaataaag tctaacaaac caccacccat gttacctaca acaccaaatg
     7021 cgtacattaa agtatccatt gatgctgcta aaactgcatg taaaacaaat aagtatggcg
     7081 cgatgaataa gaatgtaaat tctaatggct cagtaatacc tacaacgact gctgttaatg
     7141 tggcaggaat aagtagtgcc gccacctttt tacgattttc tttaggtgtc gtagcataca
     7201 ttgctaaggc aatacctaaa catccaaata ctttcccatt tccttgaagc ataaagccat
     7261 aagggaattg ctcttttaat ggcttactac tcttagcaaa ctcatttaaa tgttgtaacc
     7321 attcagcttt caagccgtga ttgactacta ctggtccaac ttcaattggc gcataaataa
     7381 agtgatgtaa tcctgttggt attaatacac gttctaagaa gtgatacaac catacaccga
     7441 tatagcctga agcaatgata aagtgttgca ttgagccaat accgtgctga acagttggcc
     7501 aaataacaca agtaattgct gctaatggta acatcacaaa aaatgagatt gtaactacaa
     7561 atgttaaacc ttggaatact ccaaccattt ctggtaatcg tttactatag tatctattgt
     7621 gaatccaagt aataatagct gaaataataa taccacctag aatattcgta tccaatgtgg
     7681 caatacctgc aattgatttt aaaccaggta cattttcaac gcctttttct aaattagcgc
     7741 caaacgtatg tggccattga gttaaaatgg catttataaa tgtattaaac attaagtaac
     7801 ccatcaatgc tgcaagtgct gcatgacctg gtgctttttt agctaaagaa agtggtaaac
     7861 ctacaacaaa tacaacttcc atatgcgtaa aaataaccca gccaccagat tctattactg
     7921 accaaaattt aaaccaaaac gtatgttgat ctgctaaact ccccatgatt gtaggatttt
     7981 taaataatgt cgcaaagcca agcactatcc caaagaaagc gaacattaat actggtacaa
     8041 tcattgcact accgaaacgc tttatcgcat tcatcttcta ttccctccat atcatctttc
     8101 ctaacaatac atctaattag attcatttat aaatagatgc cttactattt aaatataata
     8161 tatagtaaac gcttacacac ctacaacgac attgacgtat tttgaaagta ttttgtataa
     8221 tcagattatc ttttcatata gtgaaaattt tttcacaagc atgtcgagaa tatgctcgat
     8281 ttt
//
LOCUS       DATTQF010000106         8088 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.106, whole genome shotgun sequence.
ACCESSION   DATTQF010000106 DATTQF010000000
VERSION     DATTQF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.106"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(351..1178)
                     /locus_tag="VB241_002018"
     CDS             complement(351..1178)
                     /locus_tag="VB241_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1245297.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVETRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(1181..1300)
                     /locus_tag="VB241_002019"
     CDS             complement(1181..1300)
                     /locus_tag="VB241_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245298.1"
                     /translation="MMLISFVYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1412..2413
                     /locus_tag="VB241_002020"
     CDS             1412..2413
                     /locus_tag="VB241_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HEP1245299.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAAKEFVKPAGLKRVGIIAALGATVMLCLSTIKKLCRK"
     gene            complement(2535..2999)
                     /locus_tag="VB241_002021"
     CDS             complement(2535..2999)
                     /locus_tag="VB241_002021"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEP1245300.1"
                     /translation="MNFEGKLIGKELKVVIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHFDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(3012..4193)
                     /gene="ribB"
                     /locus_tag="VB241_002022"
     CDS             complement(3012..4193)
                     /gene="ribB"
                     /locus_tag="VB241_002022"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEP1245301.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHKLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(4204..4836)
                     /gene="ribE"
                     /locus_tag="VB241_002023"
     CDS             complement(4204..4836)
                     /gene="ribE"
                     /locus_tag="VB241_002023"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEP1245302.1"
                     /translation="MFTGIAEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINKQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4843..5886)
                     /gene="ribD"
                     /locus_tag="VB241_002024"
     CDS             complement(4843..5886)
                     /gene="ribD"
                     /locus_tag="VB241_002024"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEP1245303.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETIRDHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRHTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDKFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(6013..6148)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6367..7869)
                     /locus_tag="VB241_002025"
     CDS             complement(6367..7869)
                     /locus_tag="VB241_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEP1245304.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMYTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQTYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSANLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFEKLVHRRTGNHIADLKYDLARPTEMGIFQSIIEYLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQEQRSDNQ"
BASE COUNT         2576 a         1477 c         1104 g         2931 t
ORIGIN      
        1 caaatcactc tattacatat taattttata atttatatac aaagaaaata tatcaatatt
       61 accattaaat tgcaacaatt ctacatatat ttcctcaaaa aagatacgca ttgaattttt
      121 attacatata actctcatga aaattgatta aactaaatta attagttacc tttaaattta
      181 acaaattaaa tcaaaccgaa tgagactaaa ttggttattt ttcaagatat gatttgcata
      241 aatcaaaaca tttcttacaa atcaaatgtg tataaaatgt gatatacatt ctcaacctta
      301 aaaaagttta aaaaaaagat gagcatctac tgtctcacct ttttagaata ttaaatttct
      361 acaatttcgt catcttcaac aataaagccc attgtattga cgctattatt taagaaagtc
      421 agaatataac gcattacttc atcgcgttct ggctcattgt gaacctcgtg gtaaaaacct
      481 tgccaagctt taaaatataa ttcaggtgtt tgatattttt ctttaaactc atcaattgcc
      541 ctagtttcaa caattaaatc cttcgttcca tacattaata gtgttggcat tggttgaatg
      601 tcatgaatat gagccatcgt atctttcatc gtctcattaa ttgtgttata ccaatgatac
      661 gttgcttttt ttaacattaa accatcgtta actgtttctt caacaatttc taaattacgt
      721 gttaaatctt ttggttctac accaacatta aaacgtgtgt cttttgaaat tttacctata
      781 tttgaaacaa gtttatcttt acgatttttt ccattctttt gaagttctag cataggagaa
      841 attaacatca tcccctcgat tggcaattct actttttcaa gtaaatttaa taaaatcaaa
      901 ccgccaagtc ctacccctaa tacataagta ggaattttat attcattagc tatctttaac
      961 cagtctagca aactttcgtg atacgtttga aagttttcaa tttgtccttt attagctctt
     1021 gaagtttgac cttgaccagg caaatctccc ataatcacat gatagccatt tcttcttaac
     1081 atcgtaataa catatgcata tcttcccgta tgttctaata tattatgagc aataacaacg
     1141 acgcctttcg catcattttc agcttcccac ttccacatta ttatactgcc cctttttcat
     1201 taatcttcaa taacataatt atagcaaatt cactatgtag atttctattt atagtattat
     1261 tgttgtccat attattatat acaaatgaaa tcaacatcaa taatagtgta attatacata
     1321 attattttta attgtttttg atgaaaacgc tttctcgaat atttttttca tgctaaactt
     1381 attgtaaaca caaggatttg gaggagtagc aatggcacta ttaaagaatt tttttatcgg
     1441 attatctaat aatagttttt taaacaacgc agcaaaaaaa gtgggcccac gtttgggtgc
     1501 caataaagtc gttgccggaa atacaattcc agagttaatt aatacaatcg aatacttaaa
     1561 tgacaagaat atcgctgtta cggtagacaa tttaggggaa tttgtcggta cagttgaaga
     1621 aagtaatcat gctaaagaac aaattttaac aattatggac gcgcttcatc aacatggcgt
     1681 aaaggcacat atgtctgtta aattgagtca gttaggtgca gaattcgact tagaattagc
     1741 ttaccaaaat ttaagagaga ttttacttaa agcaaatact tacaataata tgcatataaa
     1801 tattgatact gaaaaatatg ctagcctgca acaaattgtt caagttttag atcgattaaa
     1861 aggcgaattt agaaatgttg gtactgtaat tcaagcatat ttatacgata gccatgaatt
     1921 agttgataag taccaagatt tacgattacg tttggttaaa ggtgcatata aagaaaacga
     1981 atcaattgca tttcaatcta aggaagacgt agatgcaaat tacatcaaga taattgaaca
     2041 acgtttgtta aacgcacgca atttcacttc aattgcaaca catgaccatc gcatcattaa
     2101 tcatgtaaaa caatttatga aagaaaatca cattgaaaaa gatcgtatgg aattccaaat
     2161 gctctatggt tttagatcag agttagcaga agaaattgca aatgaaggat ataatttcac
     2221 tatttatgta ccttatggcg atgattggtt tgcgtatttt atgagaagat tagcagaacg
     2281 cccacaaaac ctttctcttg ctgcaaaaga atttgtgaaa cctgctggct taaaacgtgt
     2341 tggcataatt gcagctttag gagctacagt tatgttatgt ttaagtacaa ttaaaaaatt
     2401 atgccgtaaa tagagcttgg cataaacaat aatttaggag tctggaacat taatcagtgt
     2461 tctaggctcc taaatattat attggcagtt ctgaatcatt tataaatctt tgattatgaa
     2521 ctcacaacag cgctctatgc ttttatagat tttaataaat tagccatttc aattgcactt
     2581 actgctgctt cggcaccttt attgccagct ttcgtacctg ctctttcaac agcttgttca
     2641 atactttcag tcgttaaaat accaaatatg actggtacat tagtttgatc atttacttta
     2701 gaaacacctt tcgcgacttc attacaaaca taatcaaaat gagacgtagc accgcgaatt
     2761 acgcatccta atgtaattac tgcatcataa tttcctgatg aggctaattt tttagctact
     2821 aaaggaattt caaacgcacc cggaacaaat gcaacatcaa tattgtcttc attaacatca
     2881 tgtcgaatca aagtatcttt tgcaccttca agtaatcttc cagtgataaa atcattaaat
     2941 cgactaacta cgattacaac tttcaattct tttccaatta atttaccttc aaaattcatg
     3001 ttaaagtcct cctatattaa atgacccatt tttatttttt tcgtttccat ataatcatga
     3061 ttatgtaccg tttctggtac gataacttca attctttctg caatatcaat gccatattgt
     3121 tttaatcctt cgaatttact tggattatta cttaataaat tgatatgttc gatgttaaaa
     3181 tattttaaaa tctgtgcagc aatatgataa tctcgcaaat cttcatcaaa acctaatgct
     3241 aaatttgcag ttactgtatc atatccttgc tcaattaatt catatgcgcg taatttgttt
     3301 aacaatccta tgccacgacc ttcttgaggt agataaataa tcatgccgcc atgttcattg
     3361 atatacttca tagacgattc aagttgagca ccacaatcac aacgttgact atggaaaata
     3421 tcgcctgtaa ggcacgcaga atgtaagcgt acattttcat gttgtcgaat tgcacctttt
     3481 gtcagtacaa ctatctcttc atctgtgtaa gtcgctttaa aaccatacat atcaaatgtt
     3541 ccgaaatctg taggcatttt cacttttgcc ttaaattcaa tttctggttc taatttttta
     3601 cgatattcaa ttaaatcatc aatcgtaatc atctttaatt tatgtttttc tttaaacttt
     3661 tgtaaatctt gtcctttcgc catcgtgccg tcatcattca taatctcaca aatgacacca
     3721 gcgggcttgg caccagtaag tttagctaaa tcaacagccg cttcagtatg accattacga
     3781 gctaataccc ctttatcttg tgctactaat ggaaataaat gaccaggacg attaaaatct
     3841 ttagcttcac tacttggatc aatgagcttt ttggcagtca atgtacgttc ataagcgcta
     3901 attcctgttg ttgtatctac atgatcaata ctcactgtaa attgcgtacc aaagatgtcg
     3961 gagttatcat caaccatttg taccaaatcc aaacgttgtg caatatcttt agacactggt
     4021 gcgcatatta atccccttgc ttctttcgcc ataaaattaa tggtattatc gttcatccat
     4081 tcagtgaccg ctactaaatc accttcattt tcacgattct catcatctac tacaataatt
     4141 gtttctccat tttttaaagc cattaaagca ctgtcaatat tatcgaattg catgctaccc
     4201 ctcctaaaaa ccaaatgctc ttaatttatc tacagataat tggtctttat ccttatttaa
     4261 aatattttca acatatttaa acaaaacgtc tgtttctaaa tgtactttat ctcctaattt
     4321 tttggatgat aaaatcgttg aacgcctcgt ttctggaata agatgaatgt caaaactgtt
     4381 atcatgtttg tcaaataccg ttagacttac accatccaca gtgatagacc cttgtttcac
     4441 taactgctta ttaatatgtt ggctacattg aatcgtaata attttggcat tggctgtttc
     4501 atttattttt gaaactgttc ctagttcatc tacatgaccg aggacaaaat gtccaccaaa
     4561 cctaccgtta ccactcatgg cacgctctaa atttacttct gattgtcgct taacatctgc
     4621 taaataggtt ttattttcag tgcctttaat tacttgtaca gtaaaagatg tctgattaaa
     4681 atcaatcact gttaaacatg caccattaac actgatggaa tcaccaatat gcatatctgc
     4741 cgtaatctta tgtgcttcga tttcaatcgt cctgactgat tgacgaattt gaacactttt
     4801 aacgacacct atttcttcag cgatgccagt aaacatgcat catcacttct ttcgtaaagt
     4861 taatttgaca ttttgattta ataactcgga atgaacaatt tcaaattggt tcgcatctgg
     4921 tatctcaatc acatcatttg tttgataaaa ttgataattt ccagatccgc caattaattt
     4981 cggggcataa tagagaataa atttgtctat ataattagat tggagaaatt ctgaagtagt
     5041 ggttggacct gcctcgacta gcaaagttcc aactcctctt ttatataaat tgtgaagaat
     5101 tgttgttaaa tcacaagact tcaagtaaat aatttcaata tgtgtttgat tggttgttaa
     5161 atttggattt tcagtatata tccaaattgg tgttgattca tcttgataaa tttgctgatt
     5221 aaaatgaata ttcccagact tagacaatat tacttttata gggttttttc catcttgaat
     5281 acgtgtagta tattgtggat catctaattc aactgtatgt cttccagtta acactgcatc
     5341 gtgtcgatgt cttaacttat agacatcttg tttaacctct ttgttagtaa tccattgact
     5401 ttgtccatta tcattcgctt gtttaccatc taaacttgca gatactttca ctgtaatttg
     5461 tggcagttgc tttgcttttg ctttaaaaaa gtcttggtat aattgtgatg cacgttcatc
     5521 atcaacgcat tcaacctcaa taccgtgatc ccgtatcgtc tcatcaccat gtgtatctaa
     5581 cgaattgtct tttgttgcgt aaactacttt agctatctta caatcaatta ttttgttaac
     5641 acagggtggc gttgaaccaa aatgactaca tggctctaac gtaatataaa tcgtcgcacc
     5701 ttcagcattt tgttgtgcca tatcaagtgc ttgaacctcc gcatgcttgt caccttttct
     5761 caagtgtgca ccaataccaa caatcctacc ttctttaact acaacagcgc caacgggtgg
     5821 attaacacct gtttgacctt gtaccatatt tgcaagttga atcgcataat ccataaattg
     5881 actcaaatga tcacctctat aaacaaaaat cctcacatca tgaattaaga tgcaaggaga
     5941 aaaatttatc gttaaataag cctatttgta cacattttta caaatacgct acattatctt
     6001 tgtcgataat taacattctt tctcccatcc agactttaac tgtcggctct agagtctcac
     6061 tagatcagcc actaatacga aacatattag caggtcgcag gcttaattta ctgccggttg
     6121 ggaatttcac cctgccccga aagaattata tatgaaattg ttatagatta tttgagtacg
     6181 tagtatgtca actacattta aaatgatact atatgttttc tgaaaaaaca attaatgacg
     6241 gttttaattt aatataatct gagtactata ggcatctcat tgctatgatt cttactaaca
     6301 gacattaaaa tcaaaacctc aatgcgtctc tatagagcgt tctctttatt atcttctagt
     6361 tacaaattat tgattgtcac ttcgctgttc ttgctcattc gattctaaag catcatataa
     6421 ttgagatact gtatgcgcaa cttgttctac tatcattttc acaccatttc gtagtttatt
     6481 aacaccgttt gtcatttgac ctatcgcaat catatttgtt aatgttccaa accttggact
     6541 aataacttga ttggtttccg gtatgatttg tatgcctccc attggatgtg cttgtacaat
     6601 ttgtctattt tcaagatttt taattaattg atcatcttga tctaattcat ttaaatgact
     6661 ttttgcacct gtcgcgttaa tgacaacatt atatatgtct actgattctt ggtttttgta
     6721 tgaaaaataa tacaacttgc catcatgttt cacatcttct aaatcttttt tcaaaattaa
     6781 agacttattt tctattaatt caataattag ttcagcagtt cttggaggca ttggatttga
     6841 atttaattga atcatctttg agtatttttg attaaattga tgttggtctt caatgcttaa
     6901 gctattccat atccaattta aattctcttt caaatattca atgatacttt ggaaaatgcc
     6961 catttctgtt ggacgcgcta aatcatactt caaatctgca atatgatttc ctgtacgtct
     7021 atgtactaat ttttcaaaat caatgtcata ttcagcacat tcttttaaaa ataaagaaac
     7081 taacgtatca agcggtgcat tgccgaaatg atgttttttt atgtcattta atttgtcttt
     7141 agttaagtac ttgaatgtca cgtctatcat tgtacctctt acacttggta aatttgcaga
     7201 acgactcgtc atagtaattg gtaatttcgg atgatgagca gcaacataac gaacaacatc
     7261 taaacttgca aggcctgtac caataatcgc aatatcgtcc agttcattta cttcgtctaa
     7321 cgtattatat gttggataag gcgtagcgat atatcctttt ttgcccttca agttatatgg
     7381 atcatggtag gcaaatgtac cacatgttaa aaatacataa tcgtacgttt gccatgattg
     7441 ttctgaattt gtagtacata tgtaataagt taaattcgtt tcatcgatat tagaatttgt
     7501 ataaatctct tgaactttat tataattagt tgatatattt ggatattttt tcgtatacat
     7561 agataaataa gatttcatat aatgtccgaa tacaaatctc ggtaaatatg caggttcatc
     7621 aaaattaaaa tcagtttgtt gtttatacca cttccaaaat tcagtctcat catctaaatt
     7681 taaactcatc tttttcgaag gcatattaat tagcagctca gaactatcat tttgaaatgg
     7741 tacgccctgt cccatattta ctttatcatc gtataaatct atatctaatt gattaaattt
     7801 cgggtgctta actaactctc tcaatacact tacaccagca gttcccatgc cgattattgc
     7861 tacacgcata tatcatccat cctttagtca ttttaaatat tttcttttat ctattaccca
     7921 tatttatagc aatttggtat ttttaataaa atttattaag gtaatctatt atttcacttt
     7981 tacaaccact aaaccattta aaacttttca tttaatatca aatctagtgt ttgtgttaaa
     8041 atatttttaa caatttgcca tattttatga attcaccatt gtcatact
//
LOCUS       DATTQF010000107         7974 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.107, whole genome shotgun sequence.
ACCESSION   DATTQF010000107 DATTQF010000000
VERSION     DATTQF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.107"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(371..817)
                     /gene="perR"
                     /locus_tag="VB241_002026"
     CDS             complement(371..817)
                     /gene="perR"
                     /locus_tag="VB241_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEP1245305.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(914..1864)
                     /locus_tag="VB241_002027"
     CDS             complement(914..1864)
                     /locus_tag="VB241_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEP1245306.1"
                     /translation="MKVVGLNRMREVETELQQRFSDVDFKFYKKASEIPESDLANLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIVINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSRVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1870..2325)
                     /locus_tag="VB241_002028"
     CDS             complement(1870..2325)
                     /locus_tag="VB241_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP1245307.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIVYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKYGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2406..3695
                     /locus_tag="VB241_002029"
     CDS             2406..3695
                     /locus_tag="VB241_002029"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEP1245308.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHSNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            3988..5082
                     /locus_tag="VB241_002030"
     CDS             3988..5082
                     /locus_tag="VB241_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP1245309.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5273..7009)
                     /locus_tag="VB241_002031"
     CDS             complement(5273..7009)
                     /locus_tag="VB241_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HEP1245310.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7300..7842)
                     /locus_tag="VB241_002032"
     CDS             complement(7300..7842)
                     /locus_tag="VB241_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP1245311.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKDRYKKIRQY"
BASE COUNT         2627 a         1352 c         1130 g         2865 t
ORIGIN      
        1 tttaaaaatt actgttgaag ggattatttt ctattcgtaa gtactatatg tatttaaagt
       61 gtaattcctt acgcttttta cttcagtttt acaaacatac ttatcctcac tttatggata
      121 gttatttagt aaaccttcgt ttgaatattt ttatgtttat ttataaagga tactagttat
      181 tagttaatac cacttccgta gtaagaatgt tcttttcttc tatttaaata tttttcagaa
      241 taagcataaa aaagaataag cataaaaaag aagttaaaca actttcatgt cgtttaactt
      301 cttaaatatt acgcttactt atatgaagta agctaacgcc tttttaattt gtcatactac
      361 caaagttaaa ttatttatct tggcattctt tacaaactcc ataaatttcc attcgatgat
      421 gtgttacgtc aaagtcagtc atatgctgag ctaatctttc aatttcattt aactgtggat
      481 attgaaaatc aacaatctta ccacattgtt cacatataat atgataatga ttatgtgtat
      541 taaagtcgaa tcgacttgat gagtctccat atgttaattc ttttacaatt ccaatatctt
      601 taaacactct taagttatta tatattgtcg caacacttat atttggaaaa tcaggtgaaa
      661 gtgcttgata aatttcatca gctgttggat gagtatgtga agaaattaaa taacgtaata
      721 ttgcttgtct ttgaggtgta attcttacgc ctgcttgtcg caatgatgca attgattctt
      781 ctagttcatg ttcaattgat tctatttcaa cactcatcta tatcaccatc tttcttatat
      841 aataattatt attacttact ataaatataa ctgtagtatt gcgcgcgtgt caatatttac
      901 atgatgattt catttaatag ccttttttag catcaacttc attctcaatt agaccattct
      961 tattgagaaa attaactaga ttgtttttaa aaatatctaa taagtcatac tttgcttcat
     1021 aatcattacc agttatatgc gctgttatag ttacattatc caattcatat aattcatgat
     1081 taggtttcaa aggttcattt tcaaacacat ctaaatatgc atgtcgaata actctacttt
     1141 ttaatacttc tattaagagc gcttctttaa ctatgctacc tcgtcctata tttataaaaa
     1201 gtgcttcatc tttcattaat tcaaaatgtt ttttctttag taaatgaatc gtttcttgcg
     1261 tttctggtaa agcatttata acaatgtcag catttggtaa tgtgctttca agtgattcga
     1321 tagtatatat ctcatcaaac tcatctttgt tctgacctga cttgctcaga ccaattaaat
     1381 tcatattaaa agcctttgct aacttcgcag ttctagtagc aattgcacct gtacctaaaa
     1441 ataaaactgt ttgtcctgat aggcgtttac cagttatttt cgaatcatat atatgttgtc
     1501 gttggttatc atatgatagt ttcatctttt tataatcatc taaaatgaaa gccaaaatgt
     1561 attcagataa ttgtttagct tgaacacctt ttccattagt taaaagtatg ccgtgatctg
     1621 caatataatc taacggtaat gtatttacac ccgttgcaaa ccatgcgatc cattttaaat
     1681 ttggacaacg tcgtaaaaat gcctcattga taccgccatc ataaccaact aatatatcta
     1741 aattagccaa gtcgctctca ggtatttctg atgctttttt ataaaattta aaatctacat
     1801 ctgaaaagcg ttgttgtaat tcagtttcaa cttcacgcat acgattcaac ccaacaactt
     1861 tcatatatgt caccccaaaa tgtttttaag ttcttctatt tgtgttttaa ctttaacctt
     1921 ctcgataaca tctaatactt taccttgttc atctattata aaagtcgttc ttacaatgcc
     1981 catactttct ttgccaaatg atttttttaa ctgatatacg ccagtttctt tagctaattt
     2041 aaaatcttca tctactaata aatcgaaatt caatccgtat ttttcaataa aattttggtg
     2101 ttttttcttt gaatcaccgc ttataccata tactgcaaca tctaaatcat tgaacatttc
     2161 taaattgtct ctaaagtcac aagcttctgt ggtgcaagta ggtgtattat ctctaggata
     2221 aaaatataca atcgcctttt taccttttaa tgtatcattt gtaatgacag ttccgtcctg
     2281 attttctaat ttaaatattg gaaattgttc tcctttttgc aacatataat cacctttttc
     2341 ttcattataa taattttatg atacgatatt agatgaaaat attaaattaa aagaggttga
     2401 tataaatgaa ttttagtgaa agtgaacgtt tacaacaact ttcaaacgaa tatattctag
     2461 gcggtgtcaa ttccccttct cgttcttata aagctgtagg aggcggtgca cctgttgtta
     2521 tgaaagaagg acacggtgca tatttatatg atgtcgatgg caataaattt attgattatc
     2581 ttcaagcata cggtccaatt attacggggc atgcacatcc tcatattact aaagcaattc
     2641 aagaacaagc tgctaaaggt gttttatttg gtacaccgac tgaactagaa attgaattca
     2701 gcaaaaaatt acgtgatgca attccatctc ttgagaaaat tcgctttgta aattctggaa
     2761 cagaagcagt catgacaaca attcgtgttg cacgtgcata tactaaaaga aataaaatta
     2821 taaaatttgc tggatcttat catggccatt ctgatttagt attggttgca gcaggtagcg
     2881 gcccatctca actcggttct ccagattcag ctggtgttcc agaaagcgta gcacgtgaag
     2941 tcattactgt tcctttcaat gatattaacg catataaaga agcaattgaa ttttggggtg
     3001 atgaaattgc cgcagtatta gtagaaccaa ttgttggtaa ctttggaatg gtaatgcctc
     3061 aacctggatt tttagaagag gttaatgaaa tttcacatag caacgggaca ctagtgattt
     3121 atgatgaagt aattactgca ttccgtttcc attacggtgc cgctcaagat ttattaggtg
     3181 ttatccctga tttaactgca tttggtaaaa ttgttggcgg tggtttacca attggaggct
     3241 atggtggacg tcaagatatt atggaacaag tagcacctct aggacctgca tatcaagctg
     3301 gtacaatggc tggtaatccg ttatctatga aagcaggtat tgcattactc gaagtactag
     3361 aacaagacgg tgtttatgaa aaattagaca gcttaggcca acaactagaa gaaggtttac
     3421 ttaaattaat cgaaaaacat aatatcacag ctacaattaa tcgtatttac ggatctttaa
     3481 cattgtactt tacagatgaa aaagtcacac attatgatca agttgaacat tctgacggcg
     3541 aagcgttcgg taaatttttc aaattgatgt taaatcaagg tatcaattta gcaccttcta
     3601 agtttgaagc ttggttctta acaactgaac atacagaaga agatattaaa caaactttaa
     3661 aagctgcaga ctatgctttt agtcaaatga aataactttt aaggaaatat attttaaatt
     3721 gaattaaaat aaaacaaatg ctcacatagt agattcattt tatatcgtta actattaacg
     3781 tttaaattga tatgtcccta tgtgagtttt tattttacaa atttatacta ttttaatctc
     3841 tgactaaagt tttacatcgc tatcagtatt atgtatgatt tattttaaca actataaata
     3901 agcttgaatt ttgtaactag taagtgtata acagtaatga atacgaatgt tagatattca
     3961 ataaacttaa aaggggcgtc attatttttg agactaggag ctcggatttt caaaactggt
     4021 atagccatta ttttagctat gtctatcgct tctttactac cggatgatgt tggtctgaaa
     4081 gccttagctg gtgtcagtgc tgttgttgca atgcaaccta gtatttatcg ttcatttaaa
     4141 acagtttctg atcaagcatt gggtaacatt ataggtgcca tactatctgt tacaatggta
     4201 acaattttta gtgataattt tatcattatg ggtgttaccg tcatagtact tattgctatt
     4261 ttatttaaat ttaatttagc acatgttgct actttggcga gtgtaacagc attaatcatt
     4321 atgggacaac ataccggttc tttctatatt actgcctttt acaggtttgt acttgtaatg
     4381 attggtgtca taagttcatc tctagttaac tttgtgtttc taccacctaa atttgaaaca
     4441 aaaatttact ataattcatt aaatatatcc tcagatattt ttatgtggtt taaattagta
     4501 ttaaatgata cgacggagtt taataatatt aaacaagata gtcataattt aaaacaacgt
     4561 gtcgaaaagt tagagaaaat ttatgattac tatagcgaag aacgtcctat tacaaaaaaa
     4621 catattcacc aacagaatag aaagaaaata cttttcagag aagtagttca gacgactaga
     4681 caagcttatg aagtattaaa caaattgtcg cgttatcaaa acgacttata tttacttaat
     4741 aacaatttcc tattacaaat caaattagat ttagattcat taactgcttt tcatgagcaa
     4801 attttagcta gcctttctaa aaaagcacgt tataatgtga cacatgttga ttatgagtta
     4861 gataatccgc agaaaaaaga tttattgtct acatttcagc atgagttaat aaatcaccct
     4921 tatcaaacgg aatattcgtt tgccaatgtt atgcaaattg ttgcagccat cgaagagtat
     4981 cgacaccatt tagagcattt agaccgcata agaattagct ttttcacata tcatagatct
     5041 gatgcagaca ttgaaattgt tgaagaagac tttgatttat aacattaaaa caattcctgt
     5101 aattaatacc acaatgagat aaagtaatat tgaatctcga aattaaattt aaaaaagcag
     5161 taagattatt ttcaatgaag aaaatctctt actgcttttt ctatgttatg acctcaccat
     5221 cctgttacaa atcttcccta caaaattgta ctgaagtcaa acaactaata ctttataaat
     5281 tttgaatgct atataaatgc tcgtaagcac cttgttttgc aatcaattca cgatgcgtac
     5341 ctgtttcaac aatatgtcca ttttcaatta cgacaatttt gtcagcatgt gtaatagtgg
     5401 acaagcgatg cgctacgata agtgtcgttc gatctttact caacacatct aatgcttctt
     5461 gaataatgga ttcactttct aaatcaagtg cacttgttgc ttcatccagg ataagaattg
     5521 gcggattatt taaaaatatt ctagcaatcg ataatctttg tttttgacca cctgataatt
     5581 taacacctcg ttcacctact tcagtgtcat atccctgtgg caagttcatg ataaagtcat
     5641 gtgcattagc cattttcgcc gcttcaacta cttcttcatc tgttgctgtt ggacgaccaa
     5701 gtaaaatatt ttccttaact gtatcagaaa ataaaatatt atcctgttgc accaatccta
     5761 tttgatttct taaacttccc gttaaaaaat ctttaatgtt gtgaccatct attaaaattt
     5821 gcccagaagt tacatcgtaa aatctcggta ttaagttaat taatgttgat ttaccaccac
     5881 cactcatacc tacgaaagca actgtttctc ctttttcaat actcaaatta atatctttta
     5941 aaattggagc ttcgttatcg ttatattgaa aactaacatg atcaatatca atacgacctt
     6001 gtttaatttc aataggttga gcaccaacac catttttaat gtcataatct tcgtcaatta
     6061 attggaatac acggtccatt gaagcaaaac tttgcgttaa agttgtaaat gatgcgacta
     6121 aacgacgtaa aggcccgaac aataactcca agtatccaac aaatgctgca agtgtaccta
     6181 ctgtgattga tccagaaata gcaagatatg caccaacacc gatgacaatt attggtccaa
     6241 tatctgtaac tgtattaatt gcggcaaaag aataggcatt ccatcttgta tgtttcaacg
     6301 cacgtgttaa gaaattagtg ttctttttat caaagttttt cgcttcattg tcttcaatcg
     6361 caaaactttt aacgactgaa ataccttgaa cacgttcatg caagaatcct tgaacctcag
     6421 ctaatgcttg agatctttca cgcgtcaatt ttcttaatct tccaaagaaa acgtacaccg
     6481 ttaaaatgta aaatgggaag ataaacagtg ctgctaaagt caatttcaca tctaaaaaga
     6541 acattatgga tagtgcaata ataattgtta tacaatctaa ccaaatattc attaacccgg
     6601 ttaaaatgaa atcttttgtt tgttcaacat cattaatcac tctagatatt acttgaccta
     6661 cttgattatt agcataaaat ctcgcactta aagcttgtaa atggttgtat aactttttac
     6721 gtatatcata caatatttta ttacttgtcc attgcgccaa atattgacgt ataaattcaa
     6781 ttggtggtct cactattaca aaaataaata atgcgatacc aatggcaata gttaaatgat
     6841 gaactttttc atcagtcgtt aatgcgtggt tattaatcac gccatctatt gcatatttaa
     6901 ttaaaagtgg tataagcatt ggtataccaa acttaattat cccaacaata atcgttgcaa
     6961 aaatacgata tttgtatggc ttaacaaatt gcaaatatcg tttaatcata caatcccccc
     7021 taatctattg ccctatccta ttcataagca taaaaatgaa tagaggttgg atacataatt
     7081 tgtagatgta aattcttctt acaatttaca tttttaaaat taagatatct caacctctgt
     7141 catattcatg tgattaaata cgttgtctta ttcgtatgtt atattctatt caatttaatc
     7201 tatggatact gtgtccccac acgacagcaa aagttatcat acttctttac atcactaagt
     7261 caatataaat gatttaatca gtatttacac tttatttgct taatattgtc taattttttt
     7321 gtaacggtct ttccaaactt tgataaaatc tggcgcaaat gggcccttct tttgttctat
     7381 ccattgttga agaatgtcca cgttgcgtct taaaataata tcaatatcat gcggataatt
     7441 catttgattc atatgttgct catattcatc ttcatctaat aaatgatact ttccatttgg
     7501 atatacttta atatctaaat catagtctat atattttaat gcctcttcat cacaaacaaa
     7561 tggcgatgac aaattgcaat agtaataaat tccatcttct ctaaacatgc agataacatt
     7621 aaaccaatat tctgagtgaa agtaaacaat tgccggttca cgtgttatcc aagttcttcc
     7681 gtcactttca gttactaacg tatgatcatt gccaccaatg acaacatgat cagtaccctt
     7741 taatattgtt gtttcagacc aaacgcgatg aatcttacca tcatgtttat aactctgaat
     7801 tttaatgttt tccccttctt taggtatgga ttctctgacc atactccaca ccaccttctg
     7861 ttaatttaac cattataaat tatagcatat ttcagaaata gtattatata aatacatatt
     7921 tttacgaaat aagattttac tacttaatga ttaaactcgg taatattgct aagt
//
LOCUS       DATTQF010000108         7890 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.108, whole genome shotgun sequence.
ACCESSION   DATTQF010000108 DATTQF010000000
VERSION     DATTQF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.108"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(226..522)
                     /locus_tag="VB241_002033"
     CDS             complement(226..522)
                     /locus_tag="VB241_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HEP1245312.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVIAEKDLWYFEDDHIIVNVVDHEDEISYSKK"
     gene            complement(900..1508)
                     /gene="plsY"
                     /locus_tag="VB241_002034"
     CDS             complement(900..1508)
                     /gene="plsY"
                     /locus_tag="VB241_002034"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP1245313.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            1714..3705
                     /gene="parE"
                     /locus_tag="VB241_002035"
     CDS             1714..3705
                     /gene="parE"
                     /locus_tag="VB241_002035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP1245314.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            3705..6107
                     /gene="parC"
                     /locus_tag="VB241_002036"
     CDS             3705..6107
                     /gene="parC"
                     /locus_tag="VB241_002036"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP1245315.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFISNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALGGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSYNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITIITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            6357..7817
                     /locus_tag="VB241_002037"
     CDS             6357..7817
                     /locus_tag="VB241_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEP1245316.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCFVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         2788 a         1136 c         1486 g         2480 t
ORIGIN      
        1 gtattgattc tttataagtt actataaatg tttggagagg caatattaat atttaatcta
       61 taaaaaatag tcaatacgtt aacattaaca taattagcgt gaagataagc taatatatat
      121 agcaaaaacg aacttgttca tacaacatca gatgtgctaa accaaagtac aacttccgag
      181 gttcagtaca catcagaatg tgtaaacaag ttcgttttat tttagttatt ttttggaata
      241 agaaatttca tcttcgtgat caactacatt tacaataatg tggtcatctt caaagtacca
      301 caaatctttt tccgctatga caacatttaa atcgtcatat tgttgttcat agccaatatc
      361 aacatcttcc tttggttcaa ctgtaaaagc aggactaaat ccttgcttga gttggaattc
      421 gccaccatat cttacaaaaa acacgagcac tttattattt tcaggtaact caagttcatt
      481 tttaaaccaa gttactgctg catcagtaag ttctatttgc ataacaatga cctcctatat
      541 ttattacaga atgggagtgg gcccaaactc gtattgtttg tggaatttct tttcgaaatt
      601 ctctgtgttg gagcccaccc caacctgcac attattgtaa gctgaatttc cataagcttc
      661 tatgttggga ccgccgacca gaattgaaaa aagctagtta taagcgcatt tcgttctgtc
      721 aactacagct aatataacat tgtggaacct atgacattga tttatgtctc ggtctcaaat
      781 gttcttgtaa aaaaactaag tatagttgaa ttttaagtta aaaaaattgt ttttgcaaaa
      841 ataaaataat ccatcaactg caaaaaaata tgcaatgatg gactaatgtc aaaaaattgt
      901 tacatccatt ttattttagg ttcttcgcct ctaaaaatcc ttgcgatatt agagcgatgt
      961 ctaattatca atatgattga aactaagaaa ctaacgacta ataaaatata gtcttgaatg
     1021 ataagcgagc caatcacaca gcaaattgct gcaacgatac ttgctaaaga aacatattta
     1081 aaaatcttca atacaataaa gaagataatt gcaagtatta gtaatagtat cgggttgact
     1141 cccaagacga cacctgcact agttgcaaca gctttgccac cttggaattt taaataaact
     1201 gggtaaacgt gtccaagtat agcgaataag cctacaatta aaccatttgt aaaaaaagta
     1261 ctaatagggc catctgcgtg aactggtaac cataaaggga agaaaacagt tatgaaccct
     1321 ttgaaaatat ctagaaatgt taccaagaat cctgcaggac gacctaatac tctaaagcta
     1381 ttagtagcgc cagtattacc actaccaaat tgtctaatat cttttttgaa aaataatttt
     1441 ccaattacga atccacttgg gaaagcgccg ataagataac ttagtagtaa catgacgatt
     1501 atcatcataa atattacaca tcctttaata tcttaggact attttatcat attcgtttaa
     1561 attacggcta aaatttttaa aaacggggat taatatatgg aattaagcta tgaaagttaa
     1621 ttgacacttg cattttacgc tgatttatat aagaataact attgtatagt tttaaaaacg
     1681 aacgtacgtt tgcaggaggc gaaatcattg gcaatgaata aacaaaataa ttattcagat
     1741 gattcaatac aggttttaga ggggttagaa gcagttcgta aaagacctgg tatgtatatt
     1801 ggatcaactg ataaacgggg attacatcat ctagtatatg aaattgtcga taactccgtc
     1861 gatgaagtat tgaatggtta cggtaacgaa atagatgtaa caattaataa agatggtagt
     1921 atttctatag aagataatgg acgtggtatg ccaacaggta tacataaatc aggtaaaccg
     1981 acagtcgaag ttatctttac tgttttacat gcaggaggta aatttggaca aggcggctat
     2041 aaaacttcag gtggtcttca cggcgttggt gcttcagttg taaatgcatt gagtgaatgg
     2101 cttgaagttg aaatccatcg agatggtaat atatatcatc aaagttttaa aaacggtggt
     2161 tcgccatctt ctggtttagt gaaaaaaggt aaaactaaga aaacaggtac caaagtaaca
     2221 tttaaacctg atgacacaat ttttaaagca tctacatcat ttaattttga tgttttaagt
     2281 gaacgactac aagagtctgc gttcttattg aaaaatttaa aaataacgct taatgattta
     2341 cgcagtggta aagagcgtca agagcattac cattatgaag aaggaatcaa agaatttgtt
     2401 agttatgtca atgaaggaaa agaagttttg catgacgtgg ctacattttc aggtgaagca
     2461 aatggtatag aggtagacgt agctttccaa tataatgatc aatattcaga aagtatttta
     2521 agttttgtaa ataatgtacg tactaaagat ggtggtacac atgaagttgg ttttaaaaca
     2581 gcaatgacac gtgtatttaa tgattatgca cgtcgtatta atgaacttaa aacaaaagat
     2641 aaaaacttag acggtaatga tattcgtgaa ggtttaacag ctgttgtgtc tgtacgtatt
     2701 ccagaagaat tactacaatt tgaaggacaa acgaaatcta aattgggtac ttctgaagca
     2761 agaagtgctg ttgattcagt agttgcagac aaattaccat tctatttaga agaaaaagga
     2821 caattgtcta aatcacttgt aaaaaaagca attaaagcac aacaagcaag ggaagctgca
     2881 cgtaaagctc gtgaagatgc tcgttcaggt aagaaaaaca agcgtaaaga cactttgcta
     2941 tctggtaaat taacacctgc acaaagtaaa aatacagata aaaatgaatt gtatttagtc
     3001 gaaggtgatt ctgcgggagg ttcagcaaaa cttggacgag accgcaaatt ccaagcgata
     3061 ttaccattac gtggtaaggt aattaataca gagaaagcac gtctggaaga tatttttaaa
     3121 aatgaagaaa ttaatacaat tatccacaca atcggggcag gcgttggtac tgactttaaa
     3181 attgaagata gtaactataa tcgtgtaatt attatgactg atgctgatac tgatggtgcg
     3241 catattcaag tgctattgtt aacattcttc ttcaaatata tgaaaccgct tgttcaagca
     3301 ggtcgtgtat ttattgcttt acctccactt tataaattgg aaaaaggtaa aggcaaaaca
     3361 aagcgagttg aatacgcttg gacagacgaa gagcttaata aattacaaaa agaacttggt
     3421 aagggcttca cgttacaacg ttacaaaggt ttgggtgaga tgaaccctga acaattatgg
     3481 gaaacgacga tgaacccaga aacacgaact ttaattcgtg tacaagttga agatgaagtg
     3541 cgttcatcta aacgtgtaac aacattaatg ggtgacaaag tacaacctag acgtgaatgg
     3601 attgaaaagc atgttgagtt tggtatgcaa gaggaccaaa gtattttaga taattctgaa
     3661 gtacaagtgc ttgaaaatga tcaatttgat gaggaggaaa tctagtgagt gaaataattc
     3721 aagatttatc acttgaagat gttttaggtg atcgctttgg aagatatagt aaatatatta
     3781 ttcaagagcg tgcattgcca gatgttcgtg atggtttaaa accagtacaa cgtcgtattt
     3841 tatatgcaat gtattcaagt ggtaatacac acgataaaaa tttccgtaaa agtgcgaaaa
     3901 cagtcggtga tgttattggt caatatcatc cacatggaga ctcctcagtg tacgaagcaa
     3961 tggtccgttt aagtcaagac tggaagttac gacatgtctt aatagaaatg catggtaata
     4021 atggtagtat cgataatgat ccaccagcgg caatgcgtta cactgaagct aagttaagct
     4081 tactagctga agagttatta cgtgatatta ataaagagac agtttccttc atttcaaact
     4141 atgatgatac gacgctcgaa ccaatggtat tgccatcaag atttcctaac ttactagtga
     4201 atggttctac aggtatatct gcaggttacg cgacagatat accaccacat aatttagctg
     4261 aagtgattca agcaacactt aaatatattg ataatccgga tattacagtc aatcaattaa
     4321 tgaaatatat taaaggtcct gattttccaa ctggcggtat tattcaaggt attgatggta
     4381 ttaaaaaagc ttatgaatca ggtaaaggta gaattatagt tcgttctaaa gttgaagaag
     4441 aaactttacg caatggacgt aaacagttaa ttattactga aattccatat gaagtgaaca
     4501 aaagtagctt agtaaaacgt atcgatgaat tacgtgctga caaaaaagtc gatggtatcg
     4561 ttgaagtacg tgatgaaact gatagaactg gtttacgaat agcaattgaa ttgaaaaaag
     4621 atgtgaacag tgaatcaatc aaaaattatc tttataaaaa ctctgattta cagatttcat
     4681 ataatttcaa catggtcgct attagtgatg gtcgtccaaa attgatgggt attcgtcaaa
     4741 ttatagatag ttatttaaat catcaaattg aggttgttgc aaatagaacg aagtttgaat
     4801 tagataatgc tgaaaaacgc atgcatatcg ttgaaggttt gattaaagcg ttgtcaattt
     4861 tagataaagt aatcgaattg attcgtagct ctaaaaacaa gcgtgacgct aaagaaaacc
     4921 ttatcgaagt atacgagttc acagaagaac aggctgaagc aattgtaatg ttacagttat
     4981 atcgtttaac aaacactgac atagttgcgc ttggaggtga acataaagaa cttgaagcat
     5041 taatcaaaca attacgtcat attcttgata accatgatgc attattgaat gtcatcaaag
     5101 aagaattgaa tgaaattaaa aagaaattca aatctgaacg actgtcttta attgaagcag
     5161 aaattgaaga aattaaaatt gacaaagaag ttatggtgcc tagtgaagaa gttattttaa
     5221 gtatgacacg tcatggatat attaaacgta cttctattcg tagctataat gctagcggtg
     5281 ttgaagatat tggtttaaaa gatggtgaca gtttacttaa acatcaagaa gtaaatacgc
     5341 aagataccgt actagtattt acaaataaag gtcgttatct atttataccg gttcataaat
     5401 tagcagatat tcgttggaaa gaattggggc aacatgtatc acaaatagtt cctatcgaag
     5461 aagatgaagt ggttattaat gtctttaatg aaaaggactt taatacagat gcattttatg
     5521 tttttgcgac tcaaaatggc atgattaaga aaagtacagt gcctctattt aaaacaacgc
     5581 gttttaataa acctttaatt gctactaaag ttaaagaaaa tgatgatttg attagtgtta
     5641 tgcgctttga aaaagatcaa ttaattacca tcattacaaa taaaggtatg tctttaactt
     5701 ataatacaag tgaactatca gataccggat taagggcagc tggtgttaaa tcaataaatc
     5761 ttaaagcaga agatttcgtt gttatgacag aaggtgtttc tgaaaatgat actatattga
     5821 tggccacaca acgcggctcg ttaaaacgta ttagttttaa aatcttacaa gttgctaaaa
     5881 gagcacaacg tggaataact ttattaaaag aattaaagaa aaatccacat cgtatagtag
     5941 ctgcacatgt agtgacaggt gaacatagtc aatatacatt atattcaaaa tcaaacgaag
     6001 aacatggttt aattaatgat attcataaat ctgaacaata tacaaatggc tcattcattg
     6061 tagataccga tgattttggt gaagtaatag acatgtatat tagctaaaaa ctatatgcaa
     6121 taacgaaatt aaatgataaa atacagtaat gttaaatttt gactaaattc aagggattta
     6181 tattaaatgc tgaccaagta cttatcgtta aaatagcgat atactttgac caaactaata
     6241 taaatctctt gtattgtata agaaatgatg atgtatctct atatttactg aagtttcaat
     6301 tcggtctaat tttatagaag gtgcatgatt catgtagagg atgcgagggg tttattttga
     6361 aagatttcga tagtttaatt cctggatggt ttaaagaatt tgtccatgtt ggtaccgatt
     6421 taatatggtc tcaatattta attggtctat tattgacagc tggattcttc tttacaatta
     6481 gttctaaatt cgtccaatta cgaatgttac ctgaaatgtt tagagcttta gtagaacgtc
     6541 cagaaacttt agaagatggt aagaagggta tttcgccatt ccaagcattt gcgattagtg
     6601 ctggttcgag agttggtact ggtaatattg ctggtgttgc gactgcgatt gttttaggtg
     6661 gtccaggtgc agtattttgg atgtgggtta ttgcatttat aggtgcagcg agtgcattta
     6721 tagaagcgac tttggctcag gtttataaag tacatgataa agatggtgga ttccgtggtg
     6781 gtccagctta ctatattact aaaggtttaa atcaaaaatg gctaggtatc gtatttgcga
     6841 ttttaattac aattacattt gcatttgtat ttaacacagt gcaatctaat acaattgcgg
     6901 agtcgttaaa tacgcaatat aatattagtc cagtaatcac aggtattatt ttagcaatcg
     6961 taactgctat tattatattt ggtggtgtac gtagtattgc tacgttatct tcgttaattg
     7021 taccgattat ggctattatt tacattggta tggttttagt aatattgcta tttaatttag
     7081 atcaaattgt tcctatgata ggtacgatta ttaaaagtgc atttggtatc gaacaagtaa
     7141 ctggtggcgc tgtaggtgct gcggttcttc aaggtattaa acgtggttta ttctctaatg
     7201 aagctggtat gggttctgcg ccgaatgcag cggcaactgc tgcagtgcca cacccggtca
     7261 agcaaggttt aatccaatca ttaggtgtgt tctttgatac aatgttggtt tgtacagcaa
     7321 ctgcaatcat gattttatta tattcaggac tgaaatttgg tgataacgca cctcaaggtg
     7381 ttgcagttac ccaatcagca cttaatgaac atttaggttc tgctggaggt attttcttaa
     7441 caatagcagt tacattattc gccttttcat ctgtggtagg taattactat tacggtcaat
     7501 ctaatattga atttttatca acaaaccgtg tgatattatt tatttttaga tgttttgttg
     7561 tagtacttgt ctttgtcggt gcagttgtaa aaacagaaac agtatggaat acagcagact
     7621 tatttatggg cttaatggca attgtaaaca ttatttccat tataggactg tccaatgtag
     7681 cttttgcatt gatgaaagat tatcaaaagc agaaaaaaga aggcaagaat cctgttttta
     7741 aacctgaaaa cttagaaatt aacttatttg gaattagtgc ttggggcgct aacaaatata
     7801 agaactctga taaataaata tttttaatgt aacgattcat tttataaaaa tcaaataaac
     7861 cctgaggttt tcaaattaat gcgaaaatct
//
LOCUS       DATTQF010000109         7847 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.109, whole genome shotgun sequence.
ACCESSION   DATTQF010000109 DATTQF010000000
VERSION     DATTQF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.109"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(80..1585)
                     /locus_tag="VB241_002038"
     CDS             complement(80..1585)
                     /locus_tag="VB241_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEP1245317.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVILAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(1925..2425)
                     /gene="queF"
                     /locus_tag="VB241_002039"
     CDS             complement(1925..2425)
                     /gene="queF"
                     /locus_tag="VB241_002039"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEP1245318.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEQMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(2445..3311)
                     /locus_tag="VB241_002040"
     CDS             complement(2445..3311)
                     /locus_tag="VB241_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEP1245319.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGKLSSQPLLLTRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQTQVLYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKVIKK"
     gene            4113..4511
                     /gene="nrdI"
                     /locus_tag="VB241_002041"
     CDS             4113..4511
                     /gene="nrdI"
                     /locus_tag="VB241_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP1245320.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            4474..6579
                     /gene="nrdE"
                     /locus_tag="VB241_002042"
     CDS             4474..6579
                     /gene="nrdE"
                     /locus_tag="VB241_002042"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP1245321.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFGGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            6699..7670
                     /gene="nrdF"
                     /locus_tag="VB241_002043"
     CDS             6699..7670
                     /gene="nrdF"
                     /locus_tag="VB241_002043"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP1245322.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
BASE COUNT         2859 a         1165 c         1317 g         2506 t
ORIGIN      
        1 gaatttaatt attagaaacc aataaaaaaa cagtaagcca tttaaatgac ttactgtttt
       61 ttgaattagg ccaacaatat taacgaatac ctttcattgc tttaataatt aaaggtgaga
      121 atgctaatac aattgttgta acaataattg caacaacacc taagaagata aagtaatttg
      181 tttgacctag tggttctatt aacttaacta atgtaccatt gattgcttgt gcagaagcgt
      241 tagttaagta ccaaatactc atcatttggg cattaaatgc tttaggtgct aacttaacag
      301 cagcactatt acccgttggt gataagcaaa gttcaccgat aacacagata atgtacgata
      361 aaataaccca gttaactgag aagtttgatg aacctgatgc ataacctaca ataccaatta
      421 gtatgtatga cgcacctgct aagaacgtac caattgcaaa tttcactggc aggctaggtt
      481 gtttagttcc aagcttttgc cataaaagtg aaataattgg agctaataat aaaataaata
      541 atgggttaat tgattggaaa atcgcttcac caaagtttgt tttccaacca aataagttta
      601 atttcatatc tgaatgttca attccataaa tgttcaatac attagaccct tgttcttgaa
      661 tagcccagaa caccattcca agaataaata atggaataaa tgctttaaca cgagaacgtt
      721 cagtatcagt gacatcttta cttctaataa ttaaagtgaa gtaaatgatt ggtaatgcaa
      781 tacctaatac taaaacagta ttactaacta agttaaatga taatgagtta gttaatgcac
      841 caataacgat aattaataca attgctaaga taacacttcc gataataaga ccatactttt
      901 tcttttcagc tggtgtcaat gggttagtag gtttcatacc aacgctacct aagtttttgc
      961 ggttgaaaag tacataccat actaaaccta atgccatacc aacagctgca atcaagaatc
     1021 caccgtggaa gtttttaaca ttaacaaagt gttgcaaaat aataggtgat aataatgcac
     1081 ccatattaac tgacatatag aaaataacaa aacctgcatc catacgtcta tcattttcag
     1141 gatataaacg gccaacgata tttgaaatgt ttggcttcat taaacctgaa ccaataatga
     1201 tgaagaacat tgatgtgaat aagccgatta atgcaaatgg taagcttaaa caaatatgtc
     1261 cgataataat aaagactgca cctaataaag tagcgcctct agtacctgta attctgtcag
     1321 caatccatcc gcctggtatt gatgtcatat agattaatga accataaact gacataattg
     1381 acatagctgt tgttttatca attccaaggc cattatctgt tacggcaaag tacatgtaga
     1441 aaatgagtag ggcacgcatg ccataataac taaacctttc ccagaactct acaaagaaga
     1501 gtacgcctag ccctcgagga tgcccgaaaa atcctgtttg aggtatgtct tgaatttgat
     1561 ttccatggga gttttgttgt gtcatttata catcccatcc tttcttcccc taatacaata
     1621 gtttaagtaa atttgattgt gccttatcaa ctaaatattt cacaaatcac aaaaaaataa
     1681 aaatattgag aatattctgt tattaagcgc gcacaattaa ttagagataa tataaaaata
     1741 tacttattga cgttaaagtg caataggttt ctataaaaat gttgtttaat tcgtgtacta
     1801 atattctttt ggtgtacttt ggtataaaat gttattttaa tgtaacctat tatgtatgct
     1861 aaatgaaaaa cacaccaata agttaggaaa atcaacttat tggtgtgttt attaaatgat
     1921 acatttaacg attatctatt ttttcgggat ataaatcatg attcattaaa cgatgttcag
     1981 ccatttgttc gtatttagaa tttggtcgtc catagtttgt ataagggtca atagaaattc
     2041 caccacgtgg tgtgaacttg ccccaaactt caatataatg tggatccata agctctatca
     2101 agtcattcat aattatattc atacaatctt catggaaatc accgtgattt ctgaaactaa
     2161 ataagtaaag tttcaacgat tttgattcaa ccattttaac atttggaata taagaaatat
     2221 agatagttgc aaaatctggt tgcccagtaa tcggacataa tgatgtgaat tctggacagt
     2281 tgaattttac gaaatagtca cgaccttgat gcttattatc aaacgattct aatacatcag
     2341 gacgatagtc aaaattgtac gtattgtctt gatttcctaa taaagttata tcttgtaatt
     2401 catcttgttg acggccatgt gccataaaaa gcgctccttt aaatttattt ttttattact
     2461 ttggcgtctc ggcgtgcttt ttcaaacatg taataacttg caccgataat aacgacgtaa
     2521 cctaatgttg catagaaatc tggagattct ccaaatagaa taaatccaag tattgctgtg
     2581 aaaattatag atgcatatgt gaaaatagaa atatctttcg cagcagcaaa actatatgcc
     2641 aatgtaacgc caatttgacc tacagctgca gctaaaccag cacctaatag ataaagcact
     2701 tgcgtttgac tcattggttc ataggtatat gcagtgaaag gtattaaaac gatgacagaa
     2761 aataaggaga agtaaaatac tatagtatat ggtgcttctc tagtactaag tgcgcgtaca
     2821 cacgtatatg ccgatgcagc aaaaatacct gaaaatagtc cagctagtga tggaatcata
     2881 gatgatgaaa attcaggttt cactattaaa agcataccta aaatagcaat tatcattgct
     2941 gttatttgat atttccttac cttttcatgt aagaaaacaa tacttaataa aatcgtccag
     3001 aaaggattga gtttcattag tgaatcagca tcgctaagta ccatatggtc aatggcgtaa
     3061 atatttaata atacacctat gagtccaagt gttgatcgtg ttaataataa tggttggctt
     3121 gagagtttac caaacattgg ttgatgatat ttatatataa aaaataatgg aataaacatt
     3181 gctactaagt ttcgtgctaa tgatttttga aaaacaggaa gatcacctgc aagtctgaaa
     3241 aacactgaca taaaactgaa accaatagcc gaaattaaaa tggcaatgat accttttact
     3301 ttaggattca attttatcgc ctcttttata taaaattaac gtatttatat tagcataaaa
     3361 caacatgttg tgcataaata gttgaaattt actataaaaa gactataata gactttatcg
     3421 aacaagtgtt ctgtgtttat ttgtcggaat aatagggcat tacactttta tgaatgtttg
     3481 tgttattaca taaaacaaat atcaattcag tatcaagcta ataagctttt tcttgatttc
     3541 tgttgataca attgagattg acacagattt taaaaaatca agtgatatct actgaaaaaa
     3601 tttttttaat ttgttcaagt ttttctaatt aagtattggt gcctagttgg aacgttttac
     3661 gaacattcga ttagaaaatg acactttaaa tcatagtgtg tcttatgtat aatgaaacac
     3721 ataatatagt gttggttaaa cgaaaaagac acaatatctt gtgttttgta tgcaaatgct
     3781 ttatttatga agaaattaca tttaaaagta atttaacaca gaaatttaat agttattatc
     3841 aatgaatagt catatttttt agaaaatgta ctgagcaaat ggaaggtatc taatgatcta
     3901 aacactacat atagtgattt ttatacattc aacccatata agctactatt ttctcaaata
     3961 taaatctatg caattggttt acatttgaga aaataagtag cttcattata gttaatacaa
     4021 tgctgagata accatagtag cgacgttgtt aaagcatttt ttaattgtaa tgactactta
     4081 atttaaaagg gttgaagaaa gaaggtgatc caatgaaaat aatatatttt tcatttactg
     4141 gaaatgtccg tcgtttcatt aagagaacag aacttgaaaa tacgcttgag attacagcag
     4201 aaaattgtat ggaaccagtt catgaaccgt ttattatcgt tactggcact attggatttg
     4261 gagaagtacc agaacccgtt caatcttttt tagaagttaa tcatcaatac atcagaggtg
     4321 tggcagctag cggtaatcga aattggggac taaatttcgc aaaagcgggt cgcacgatat
     4381 cagaagagta taatgtccct ttattaatga agtttgagtt acatggaaaa aacaaagacg
     4441 ttattgaatt taagaacaag gtgggtaatt ttaatgaaaa ccatggaaga gaaaaagtac
     4501 aatcatattg aattaaataa tgaggtcact aaacgaagag aagatggatt ctttagttta
     4561 gaaaaagacc aagaagcttt agtagcttat ttagaagaag taaaagataa aacaatcttc
     4621 ttcgacactg aaatcgagcg tttacgttat ttagtagaca acgattttta tttcaatgtg
     4681 tttgatattt atagtgaagc ggatctaatt gaaatcactg attatgcaaa atcaatcccg
     4741 tttaattttg caagttatat gtcagctagt aagtttttca aagattacgc tttgaaaaca
     4801 aatgataaaa gtcaatattt agaagactat aatcaacacg ttgcaattgt tgctttatac
     4861 ctagcaaatg gtaataaagc acaagctaaa caatttattt ctgctatggt tgaacaaaga
     4921 tatcaaccag cgacaccaac atttttaaac gccggccgtg cgcgtcgtgg tgagctagtg
     4981 tcatgtttct tattagaagt ggatgacagc ttaaattcaa ttaactttat tgattcaact
     5041 gcaaaacaat taagtaaaat tggtggcggc gtagcaatta acttatctaa attgcgtgca
     5101 cgtggtgaag cgattaaagg aattaaaggc gtagcgaaag gtgttttacc tattgctaag
     5161 tcacttgaag gtggctttag ctatgcagat caactaggtc aacgccctgg tgctggtgct
     5221 gtgtacttaa atatcttcca ttatgatgta gaagaatttt tagatactaa aaaagtaaat
     5281 gcggatgaag atttacgttt atctacaata tcaactggtt taattgttcc atctaaattc
     5341 ttcgatttag ctaaagaagg taaagacttt tatatgtttg cacctcatac agttaaagaa
     5401 gaatacggtg ttacattaga cgatattgat ttagaaaaat attatgatga tatggttgca
     5461 aatccaaatg ttgagaaaaa gaaaaagaat gcacgtgaaa tgttgaattt aattgcgcaa
     5521 acacaattac aatcaggcta tccatattta atgtttaaag ataatgctaa tagagttcat
     5581 ccgaattcaa atattggaca aattaagatg agtaacttat gtactgaaat tttccaatta
     5641 caagaaactt caattattaa cgactatggt attgaagacg aaattaaacg tgatatttct
     5701 tgtaacttag gttcattaaa cattgtaaat gtaatggaaa gcggaaaatt cagagattca
     5761 gttcactctg gtatggacgc attaactgtt gtaagtgatg tagcaaatat tcaaaatgct
     5821 ccaggagtta gaaaagctaa tagtgaatta cattcagttg gtcttggtgt gatgaattta
     5881 catggttact tagcaaaaaa taaaattggt tatgagtcag aagaagcaaa agattttgca
     5941 aatatcttct ttatgatgat gaatttctac tcaatcgaac gttcaatgga aatcgctaaa
     6001 gaacgtggta tcaaatatca agactttgaa aagtctgatt atgctaatgg caaatatttc
     6061 gagttctata caactcaaga atttgaacct caattcgaaa aagtacgtga attatttggc
     6121 ggtatggcta ttcctacttc tgaggattgg aagaaactac aacaagatgt tgaacaatat
     6181 ggtttatatc atgcatatag attagctatt gctccaacac aaagtatttc ttatgttcaa
     6241 aatgctacaa gttctgtaat gccaatagta gatcaaattg aacgtcgtac ttatggtaat
     6301 gcggaaacat tttaccctat gccattctta tcgcctcaaa caatgtggta ctacaaatct
     6361 gcattcaata ctgatcagat gaaattaatc gatttaattg cgacaattca aacgcatatt
     6421 gaccaaggta tctcaacgat actttatgtt aattctgaaa tttctacacg tgagttagca
     6481 agattatatg tatatgcgca ctataaagga ttaaaatcac tttactatac tagaaataaa
     6541 ttattaagtg tagaagaatg tacaagttgt tctatctaat aagtaaacgt aaaaaatgac
     6601 aaacaactaa tcatctcgtt taaaataaca gatgattagt ctgccatgtc tatatttgtc
     6661 aattattgag agtaacatta caggaggaaa ttatattcat gatagctgtt aattggaaca
     6721 cacaagaaga tatgacgaat atgttttgga gacaaaatat atctcaaatg tgggttgaaa
     6781 cagaatttaa agtatcaaaa gacattgcaa gttggaagac tttgtcagaa gctgaacaag
     6841 acacatttaa aaaagcatta gctggtttaa caggcttaga tacacatcaa gcagatgatg
     6901 gtatgccttt agtcatgcta catacgactg acttaagaaa aaaagcagtt tattcattta
     6961 tggcgatgat ggagcaaata cacgcgaaaa gttattcgca tattttcaca acgctattac
     7021 catctagtga aacaaactat ttattagatg aatgggtttt agaagagcca catttaaaat
     7081 ataaatcaga taaaattgtt gctaattacc acaaactttg gggtaaagaa gcttccatat
     7141 atgaccaata tatggccaga gttacgagtg tatttttaga aacattctta ttcttctctg
     7201 gtttctatta tccgctatat ttagctggtc aaggaaaaat gacgacatca ggcgaaatca
     7261 ttcgtaaaat tcttttagat gaatctattc atggtgtgtt tactggttta gatgcgcagc
     7321 atttacgaaa tgaactatct gaaagcgaga agcaaaaagc tgaccaagaa atgtataaat
     7381 tgctaaatga cttgtattta aatgaagagt catacacaaa aatgttatac gatgatcttg
     7441 gaatcactga agatgtgtta aactatgtta aatataatgg aaataaagca ctttcaaatt
     7501 taggttttga accttatttt gaggaacgtg aatttaaccc aattattgag aatgccttag
     7561 atacaacaac taaaaaccat gacttcttct ctgtaaaagg tgatggttat gtattagcat
     7621 taaatgtaga agcattacaa gatgatgact ttgtatttga caacaaataa taattaaaat
     7681 taaaaagacc ttcacatgaa agggtaatag cgattcgttt cgtcttgact cctacatgtt
     7741 gaaggtcttt tttctataac ttaaaaacgt atgcacattt tgaaaaagta aaaatggcat
     7801 ttaatgatgc caatgaggct agaaagtata caagatactg cattaca
//
LOCUS       DATTQF010000110         7821 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.110, whole genome shotgun sequence.
ACCESSION   DATTQF010000110 DATTQF010000000
VERSION     DATTQF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.110"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            262..1431
                     /locus_tag="VB241_002044"
     CDS             262..1431
                     /locus_tag="VB241_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEP1245323.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVQNQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
     gene            1707..2423
                     /locus_tag="VB241_002045"
     CDS             1707..2423
                     /locus_tag="VB241_002045"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HEP1245324.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            2416..3441
                     /locus_tag="VB241_002046"
     CDS             2416..3441
                     /locus_tag="VB241_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEP1245325.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            3463..5151
                     /locus_tag="VB241_002047"
     CDS             3463..5151
                     /locus_tag="VB241_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEP1245326.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            5184..6908
                     /gene="tarS"
                     /locus_tag="VB241_002048"
     CDS             5184..6908
                     /gene="tarS"
                     /locus_tag="VB241_002048"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEP1245327.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            7048..7722
                     /gene="scdA"
                     /locus_tag="VB241_002049"
     CDS             7048..7722
                     /gene="scdA"
                     /locus_tag="VB241_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HEP1245328.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNEVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
BASE COUNT         2883 a         1079 c         1414 g         2445 t
ORIGIN      
        1 tccaactgaa ttgtcatttt gtaaaataaa ataatcaatc caatcgttat ctaattcata
       61 aatgtgaaaa cacatacgtt atgaatggat aacgattttt tgttatgtta aagtggtaca
      121 ttaatcatgt atttcgtatg ataattaacg acaagtgtaa tggttaaatg tattttatgc
      181 tgaaatgcta taataggcat ggttacaatg agcttgctca tacatattaa tataattaca
      241 aaaacacgtc ggaggtacga catgattaaa actacaatta aaaaattgat agaacacagt
      301 atatatacga cttttaaatt actatcaaaa ttgccaaaca agaatctaat ttattttgaa
      361 agctttcatg gtaaacaata cagcgacaat cccaaagcat tatatgaata cttaactgaa
      421 catagcgatg cccaattaat atggggtgta aaaaaaggat atgaacacat attccaacag
      481 cacaatgtac catatgttac aaagttttca atgaaatggt ttttagcgat gccaagagcg
      541 aaagcgtgga tgattaacac acgtacacca gattggttat acaaatcacc gcgaacgacg
      601 tacttacaaa catggcatgg cacgccattg aaaaagattg gtttggatat tagtaatgtt
      661 aaaatgctag gaacaaatac tcaaaattac caagatggct ttaaaaaaga aagccaacgt
      721 tgggattatc tagtgtcacc taatccatat tctacatcga tatttcaaca tgcatttcat
      781 gttagtcgag ataagatttt ggaaacaggt tatccaagaa atgataaatt atcacataag
      841 cgcaatgata ctgaatatat taacggtatt aagacaagat taaatattcc attagataaa
      901 aaagtgatta tgtatgcgcc aacttggcgt gacgatgaag cgattcgaga aggttcatat
      961 caatttaatg ttaactttga tatagaagct ttgcgtcaag cgctggatga tgattatgtc
     1021 attttattac gcatgcatta tttagttgtg acacgtattg atgaacatga tgattttgtg
     1081 aaagacgttt cagattatga agacatttcg gatttatact taatcagcga tgcgttagtt
     1141 accgactact catctgtcat gttcgacttc ggtgtattaa agcgtccgca aattttctat
     1201 gcgtatgact tagataaata tggcgatgag cttagaggtt tttacatgga ttataaaaaa
     1261 gagttgccag gtccaattgt tcaaaatcaa acagcactca ttgatgcatt aaaacaaatc
     1321 gatgagactg caaatgagta tattgaagca cgaacggtat tttatcaaaa attctgttca
     1381 ttagaagatg gacacgcgtc acaacgaatt tgccaaacga tttttaagtg ataacttaaa
     1441 aacaataaaa aattataaat taattagtta agtgatataa ataataaacg aaatgtttgc
     1501 ttgtatgtta ttatttgtgt atgaaaacgt ctgatataat gtaatatagt tttaaacaaa
     1561 atatgctaaa atataagcaa aatatgtata attttaagta atacacagac atttagagtt
     1621 ttgattttaa aatgagtttt caattgaaaa atgcaacgaa atttaaataa ttaaatatag
     1681 atatagttga atggaggaag tattttatga aatacgctgg tattctagct ggaggtatag
     1741 gctcaagaat gggtaacgta cctttaccta aacaattttt agatttagac aacaaaccga
     1801 ttttaatcca tacattagaa aaatttattt taattaatga ttttgaaaaa attattatcg
     1861 cgacgccaca acaatggatg acgcatacga aagatacact tagaaaattc aaaatttctg
     1921 atgaaagaat tgaagtcatt caaggtggta gcgatcgtaa cgatacaatt atgaatatcg
     1981 ttaaacatat tgaatcaaca aatggtatta acgatgacga tgtcattgtg acacatgatg
     2041 cagttagacc atttttaacg catcgtatta ttaaagaaaa tattcaagct gctttagaat
     2101 acggtgcagt agatacagtg attgatgcta tagatacgat tgttacatct aaagataatc
     2161 aaacgattga tgcaattcca gtgcgtaatg aaatgtacca aggtcaaaca cctcaatcgt
     2221 ttaatattaa tttattaaaa gaaagctatg cacagttgag tgatgagcaa aagagtattt
     2281 tatctgatgc ttgtaagatt attgtagaaa caaacaaacc ggttcgactt gtaaaaggtg
     2341 agttatataa cattaaagta acaacacctt acgatttaaa agtagcgaat gctattattc
     2401 gaggtggtat tgccgatgat taatcaagta tatcaattag ttgcacctag acaatttgaa
     2461 gttacgtata acaacgtaga tatttacagt gactatgtca ttgtacgtcc tttatatatg
     2521 tcaatttgtg ctgccgatca aagatattat actggtagcc gtgatgagaa tgtcttatct
     2581 cagaaattgc caatgtcttt aattcatgaa ggtgttggtg aggtcgtatt tgacagtaaa
     2641 ggtgtgttta ataaaggtac aaaagtagtt atggtaccga atacaccgac agaaaaagac
     2701 gatgtcattg ctgaaaacta tttaaaatcg agctacttca gatcaagtgg acatgatggg
     2761 tttatgcaag attttgtgtt gctaaatcat gatagagctg taccactacc tgatgatatt
     2821 gatttaagta ttatttcata tacagagctt gtaacagtaa gtttgcatgc tattcgtcgt
     2881 tttgaaaaga aatctatttc aaataaaaat acatttggta tttggggtga tggtaactta
     2941 ggttacatta cagccatttt attacgtaaa ttatatccag agtctaaaat atatgtcttt
     3001 ggtaaaacag attataaatt gagtcacttc tcatttgttg atgatgtctt ctttattaat
     3061 aaaatacctg aaggcttaac atttgatcat gcatttgagt gcgttggtgg gcgtggtagc
     3121 caatcagcaa taaatcaaat gattgattat atttcaccag aaggaagcat tgcactgtta
     3181 ggtgtaagtg agttcccagt agaagttaat acacgtctcg tattggaaaa aggactaacg
     3241 ttgattggta gtagtcgaag tggttcaaaa gatttccaag atgttgtaga cttatacatt
     3301 caatacccag atattgtaga taaattagcg ttgttaaaag gtcaagaatt tgaaattgca
     3361 acaattaatg atcttacaga agcttttgaa gcagacctgt ctacatcttg gggtaaaaca
     3421 gtattaaaat ggattatgta aatagaaaag gatgaattaa cgttggttaa aagtaagata
     3481 tatatagata aaatctattg ggagcgtgtt caattattcg ttgaaggaca tagtgaaaac
     3541 ctagatttag aagatagtaa ttttgtatta agaaatttaa ctgagacacg tacaatgaaa
     3601 gcgaatgatg tcaaaataga tgggaatcaa ttcgtttgtc gtttcaatgt agctatctta
     3661 gataatggtt attacttacc tgaagataag tacttattag tgaatgagca agaacttgat
     3721 tatattgcac agttaaaccc agatgtgatt aatgatgcat atcaaaatct aaagccagaa
     3781 caagaagaag aatacaacga attagaaaca caaaatggta aaatcaattt cttattgcag
     3841 acttacctaa aagaatttag aaaaggcggc atttcaaaga aaacggttta tactgttaca
     3901 cctgaaattt ctagcgatgt taatgaattt gtccttgatg ttgttgtaac gactccggaa
     3961 gttaaaagta tttatatcgt tcgtaaatat aaagaattac gtaagtattt ccgcaaacaa
     4021 tcatttaata caagacaatt tatttttaaa gcgatattta atacgacgaa atttttccac
     4081 ttgaaaaaag ggaatacggt gttgttcaca tcagactcta gaccaacgat gtctggaaac
     4141 tttgaataca tctataacga aatgttacgt caaaatttag ataaaaagta tgatattcac
     4201 actgttttta aagcgaatat tacagataga cgtggcatca tcgacaagtt tagattgcca
     4261 tatttacttg ggaaggcaga ctacatcttt gttgatgact ttcacccatt gatttataca
     4321 gtgcgtttta gacgttctca agaagttatt caagtatggc atgccgttgg tgcctttaaa
     4381 acagttggct ttagtcgtac tggtaaaaag ggtggaccat ttattgattc attaaatcat
     4441 cgtagctata caaaagctta tgtatcatct gaaaccgata ttccattcta cgctgaagca
     4501 tttggtatta aagagaaaaa tgtagtgcct acaggtgttc cacgtactga tgtactattt
     4561 gatgaagctt atgcgacaca gatcaaacaa gagatggaag atgaattacc aattattaaa
     4621 ggtaagaaag tcattctttt cgcaccaaca tttagaggta gtggtcatgg tacagcacat
     4681 tacccatttt tcaaaattga tttcgaacgt ttagcaagat attgcgaaaa aaataacgcg
     4741 gttgtattat ttaaaatgca tccatttgtg aaaaatagac ttaatattgc agacaaacat
     4801 aaacaatatt ttgttgacgt ttctgacttt agagaagtta atgatatact gttcataaca
     4861 gatttattaa ttagtgacta ttcatcttta atatatgaat atgcagtatt taaaaagcca
     4921 atgattttct atgcatttga tttagaagat tatattacga cgcgtgattt ttatgagcca
     4981 tatgaatcat ttgttccagg taaaattgtg caatcatttg acgcattaat ggacgccttg
     5041 gacaatgaag attatgaagg agaaaaagtc attccattct tagataaaca ttttaaatat
     5101 caagatggtc gatcaagtga gcgtttagtc agaaatttat ttggtagcta agtttatata
     5161 gtagtcaaag tgggagaggt ataatgatga aattttcagt aatagttcca acatacaatt
     5221 cagaaaagta tataacagaa ttactcaata gccttgcgaa acaagatttt ccgaaaactg
     5281 aatttgaagt ggttgtagtt gatgactgtt caacagatca aacgttacaa atagttgaaa
     5341 agtatcgcaa taaattgaac ttgaaagtaa gtcaactcga aacaaattct ggtggtccag
     5401 gtaaacctag aaatgtggcg ttaaaacaag cagaaggtga atttgtatta tttgtggact
     5461 ccgatgacta tataaacaaa gagactttaa aggatgcagc agcatttatt gatgaacatc
     5521 actcagatgt cttattgatt aaaatgaaag gtgttaatgg tcgtggtgta ccacaatcta
     5581 tgtttaaaga aacagcacct gaagttactt tgttaaattc aagaattatc tatactttaa
     5641 gcccgactaa aatctataga acagcattac taaaagataa tgacatttat tttccagaag
     5701 aattaaagag tgcagaagat caattattta caatgaaagc atatttgaat gcaaatcgaa
     5761 tcagtgtgtt aagtgataaa gcgtattatt atgctacaaa gcgtgaaggt gaacatatga
     5821 gtagtgcgta tgtttcacct gaagactttt acgaagtcat gagattgatt gctgtagaaa
     5881 tattaaatgc agatttagaa gaagcccata aagatcaaat cttagcagaa tttttaaatc
     5941 gtcattttag tttttctcgt acgaatggct tctcacttaa agttaaacta gaagatcaac
     6001 cacaatggat taatgctcta ggagacttta tacaagcagt tccagaacgt gtagatgcat
     6061 tggtgatgag taaattacga ccattgttgc actacgcgag agcgaaagat atagacaact
     6121 atagaactgt agaagaaagt taccgtcaag gtcaatacta ccgttttgat attgtagatg
     6181 gtaaattaaa cattcaattc aatgaaggcg aaccatactt tgaaggcatt gatatcgcta
     6241 agccaaaagt gaaaatgaca gcatttaaat ttgataatca taaaattgtt acagagctaa
     6301 cgctaaatga atttatgatt ggcgaaggac attatgatgt cagacttaaa ttacattcac
     6361 gaaacaagaa gcacacaatg tatgtacctt taagtgtcaa tgcgaataaa caatatcgtt
     6421 ttaacattat gttagaagat attaaagcgt atttacctaa agaaaaaatt tgggatgttt
     6481 tcttagaagt ccaaataggt acggaagtat ttgaagtgcg tgttggtaat caacgtaata
     6541 aatatgctta tactgcagaa acaagtgcat taattcattt gaataatgat ttttatagat
     6601 taacaccgta tttcacaaaa gactttaata acatttcgtt atactttaca gctattacat
     6661 taacggattc aatctcaatg aagttaaaag gtaaaaacaa aatcatttta actggtctgg
     6721 atcgtggtta tgtatttgaa gaaggtatgg ctagtgtcgt gctaaaagac gacatggtga
     6781 tgggaatgtt aagccaaaca tcagaaaacg aagttcaaat cttacttagc aaagatatta
     6841 agaagcgaga cttcaaaaat attgttaagt taaatactgc acatatcact tatccactaa
     6901 ataaataata aaacccctca aatcattgtg aactaacatg atttgagggt tttattttac
     6961 tgtttatgac atgattatga catttcctcg tttatcattt tcaaatacat taaattgtat
     7021 acactggaaa tgaggaggtt atctataatg ataaataaaa atgacatagt agcagatgta
     7081 gtaactgatt atccgaaagc agcggatatt tttagaagtg tgggaataga tttttgttgc
     7141 ggcggacaag taagtataga agcagcatcc ttagaaaaga aaaatgtaga tttgaacgaa
     7201 ttattacagc gtctcaatga agttgaacaa acgaatacac caggttcgct taatcctaaa
     7261 tttttaaatg tttcatcact tattcaatat attcaatcag catatcatga acctctaaga
     7321 gaagaattta aaaatttaac accttatgtg acgaagttat cgaaagtaca tggacctaac
     7381 catccatatt tagttgagtt aaaagaaaca tacgatacat ttaaaaatgg catgttagag
     7441 catatgcaaa aagaagacga tgtcgatttt ccaaaactca ttaaatatga gcaaggtgag
     7501 gtagtagacg atattaatac tgtgatagat gatttagttt cagaccacat tgcaacggga
     7561 caattgttag taaaaatgag cgaattaaca tctagttatg aacctccgat agaagcgtgt
     7621 ggtacttggc gactcgttta tcagagatta aaagcacttg aagtgttaac acatgaacac
     7681 gtacatttag agaatcacgt attatttaaa aaagtatcat aactaaataa cgcgattaga
     7741 aactgttggc aaaaataagt ccagcagttt ttcgctatgt ataaaagtca taatagtgac
     7801 atgaacatca ttatttgaaa a
//
LOCUS       DATTQF010000111         7786 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.111, whole genome shotgun sequence.
ACCESSION   DATTQF010000111 DATTQF010000000
VERSION     DATTQF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.111"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            260..1051
                     /locus_tag="VB241_002050"
     CDS             260..1051
                     /locus_tag="VB241_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEP1245329.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            1065..2951
                     /locus_tag="VB241_002051"
     CDS             1065..2951
                     /locus_tag="VB241_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HEP1245330.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFEDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHVQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            3176..4519
                     /gene="brnQ"
                     /locus_tag="VB241_002052"
     CDS             3176..4519
                     /gene="brnQ"
                     /locus_tag="VB241_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP1245331.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAIALIFIYISLGYIGNHMPVSDMKLNELKSHDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRITQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(4587..5723)
                     /locus_tag="VB241_002053"
     CDS             complement(4587..5723)
                     /locus_tag="VB241_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP1245332.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(5755..6384)
                     /locus_tag="VB241_002054"
     CDS             complement(5755..6384)
                     /locus_tag="VB241_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEP1245333.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGIIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(6403..6672)
                     /locus_tag="VB241_002055"
     CDS             complement(6403..6672)
                     /locus_tag="VB241_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP1245334.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            6834..7142
                     /locus_tag="VB241_002056"
     CDS             6834..7142
                     /locus_tag="VB241_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP1245335.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            7313..7513
                     /gene="cspA"
                     /locus_tag="VB241_002057"
     CDS             7313..7513
                     /gene="cspA"
                     /locus_tag="VB241_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HEP1245336.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            7710..>7786
                     /locus_tag="VB241_002058"
     CDS             7710..>7786
                     /locus_tag="VB241_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HEP1245337.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNI"
BASE COUNT         2624 a         1120 c         1295 g         2747 t
ORIGIN      
        1 aactgaagaa tattatcaaa taatagataa ttcaggatta gttggcggtt actataaaac
       61 atatttcaaa gaagtttaaa cgcataatta ttgaaatgaa tcctaatatg agattttcaa
      121 ttagtaaatt tggtaaaata taaacaattc gcatactaag cattgcgttt tatatcagaa
      181 tagaatagta aactaaggca tttggagaac ttattccatg tcttagtttt ttaatttatt
      241 taaaggagaa cgttttaaaa tggcactaaa acattataag aattcagatt caacagtttt
      301 caatgatgcg aaggcattat tcgatttaaa taaaaatatt ttacttaaag gtccaacagg
      361 ttcagggaaa acgaagttgg cagaaacatt aagtgaagtt gttgatacac ccatgcatca
      421 agtcaactgt tctgttgatt tagatacaga aagcttatta ggctttaaaa caattaaaac
      481 aaatgcggaa ggacaacaag aaattgtctt tgtagatggt ccagttatta aagctatgaa
      541 agaggggcat attttatata ttgatgaaat aaatatggct aaacctgaaa cattacctgt
      601 attaaatggg gtcttagatt atcgtcgtca aattacgaat ccatacactg gtgaagtaat
      661 caaagctgta ccaggattta acgttattgc agcgataaat gaaggttatg ttggtacatt
      721 gccaatgaat gaagcactaa aaaatcgctt tgttgttatt cacgttgact atattgacgg
      781 ggacatttta aaaaatgtaa ttaaagagca aagtttatta caagatgata aacaaatcga
      841 acaaattatt aagtttaacg aagatttacg tactatgtct aagcagggac aaatttctga
      901 agaagccgct agtatacgtg cattattaga cttgtgtgat ttaatcactg taatgccagt
      961 tgaacgtgca attaaacgta caattattga taaattggaa gatgaacgtg aacaacaagc
     1021 aatatataat gctgtagaac taaactttta aagagggata aaacatgagt gatcgtttca
     1081 taaaatttaa cgacgaacag ttagatgcca agcaagtaat gatgttgcaa gacttagctc
     1141 gactcttatt aaaaaatgaa caaacacaag ttaaaatcca aaaattccct tactataatc
     1201 ctgttcaaaa tgttttgatt acgagttggt tttggtcaca tcgaccaagc catatcgaaa
     1261 tggctggttt aaaaacagat gttatgttag ctgcttacgg ttatcatatg atggatgtac
     1321 aaattgtaaa tgaagtagta caagataaaa cttttaaaca ccctaaattt tatcaacaac
     1381 tttttaaatt attagaagat atgcgcgtat taaattctat taaagttgag cgcccaagta
     1441 ctgctaaatt aattgatctt cgcttagata cacggatatc atatacggaa tctcaaatta
     1501 aagtgtatcg tactaaaaca caatatacag atttattatt tttgtattta gaacatgcat
     1561 ttttaagtca agatttcttt gatataccat ctatacattc agatttagat gatattttag
     1621 ttaatatgtt cttatattta cctaattttt tccaaaatca aaattctgaa gataatatgt
     1681 atctggctca aagaattatg tatcaagtag atgatatttt aaaagaagat atgctaaacg
     1741 aatattatta cttacctaaa acactatata atacattagc tagtccagag tttgaagatt
     1801 taaagagaac tgatgcaagt caagtcgatg ggcaagatga tacttcagaa gatgatgata
     1861 acgaaagtga aaaagctgac tctaaatctg cagatagtga gtcaaaaggt ggcgcctatt
     1921 tagaaatgga gcttcacgaa ggacaaaata gtgaaacatt aggtaatgat gaagcacgtg
     1981 aaggcgatgc tacagacgat atgactgata tgatgaccaa aaagggcaaa ggatcaaacg
     2041 atacgctaaa tcgtgaagaa ggcgatgctg ttggtcaaag ccaagcattt caacttgatg
     2101 gtgtgaacaa aaatgtagaa ataaaatggc aaattcctga aattgagcca caatatgttt
     2161 tagaatatca agaatcgaaa caagacgtac aatatgaaat caaagactta attcaaatta
     2221 taaagaaaac gattgaacga gaacaacgag atgcacgttt taatttaaca aaagggcgat
     2281 tacaaaagga tttaattaat tggtttatcg acgatcagta taaattgttt tacaaaaaac
     2341 aagatttaag taaatcattt gatgcgacgt ttacattgtt aattgatgcg tcagcaagta
     2401 tgcatgataa aatggctgaa acgaagaaag gtgtagtgtt attccacgag acgttaaaag
     2461 cattgaacat taaacatgaa attttatcat tcagtgagga tgcctttgat tcagacgatc
     2521 atgtacaacc aaatatcatt aatgaaatta ttaactatga ttactcaacc tttgaaaaag
     2581 atggcccacg tattatggca cttgaacctc aagatgataa tcgtgatggt gttgctatta
     2641 gagttgcaag tgaacgatta atgcgtcgaa atcaacatca acgattttta atcgtatttt
     2701 ctgacggcga accgtctgca tttaattata gtcaagatgg tattattgat acgtatgaag
     2761 ctgtagaaat gtcacgtaaa tttggcattg aagtatttaa tgtatttttg agtcaagatc
     2821 caataactga agatgttgaa caaacaattc ataatattta tggtcaatat gccattttcg
     2881 ttgaaggtgt tgcgcattta cctggacatc tctctccatt attaaaaaaa ttactactta
     2941 aatctttata aataagttta aaatatgagt ttatatgaaa ttgtgaataa aagtcatcag
     3001 ttactattta aaaagtaaat ttgaagaatt ttattttctg gcataatttg atttaaacaa
     3061 cacatgtgtt ttgtttctgc tatgtacaac atattccata tagactcttg attatttttt
     3121 gtgctaaaat attaacttgt aaattatttc gtgttttaaa aaaaggagta actctatgaa
     3181 taaaaataca tgggtcattg ggtttacgct cttcgctatg ttttttggcg caggcaatct
     3241 aatttttcct cctaatctcg gattggatag tggtcaattt ttctggcccg ccattttagc
     3301 gtttgttcta actgggattg gtttaccatt attaggtgtg attgtaggtg cacttgataa
     3361 agaaggatat attggcgcat taaataaaat ttcacctaaa ttttcaatat tgttcttaat
     3421 catcatttat ttaactatag gaccactttt cgcaatacct agaactgcat ctacatcttt
     3481 tgaaatgaca attacaccaa ttatacatag taatagtagt atcgctttat ttatatttac
     3541 gattatctac ttcatagtcg ttttgtatat ttgtttaaat ccatctaagt taatcgatcg
     3601 tattggttca ttattaacac cattattatt gattactatt ttagcgatga ttattaaagg
     3661 atacttagac tttagcggta atagtgctgg aaagggcaat gaagcactat atcattctaa
     3721 tttttcaagt tttgctgaag gctttacaca aggctattta acaatggatg ccattgcagc
     3781 aattgctttt tcaatgattg ttgttaatgc agtaaaacta acaggtatta ctaaaacaaa
     3841 tcaaatattc aaacaaactt tgactgctgg tttaattgca gcaattgctt taattttcat
     3901 atatatttca ttaggttata ttggtaatca tatgccagta agtgacatga aactaaatga
     3961 attgaaatca catgaccgta acattgggac atatttatta acgacaatgg cttcaacagg
     4021 atttggttca ttcggaaagt atttattggg catcattgtg gcgctggcat gtctaactac
     4081 agcatgtggg cttattgttg cagtttctga atatttccat agaatcgtac ctaaagtatc
     4141 atacaaagca tttgtattag ttttcatttt aatgagtttt attattgcta accaaggttt
     4201 aaatgctgtt atctcaatgt caattccggt attaagcatt gtatacccag tagcaataac
     4261 tgttgtatta ttaattttaa ttgccaaatt cataccgaca aaacgcatta cacaacaaat
     4321 tccagttatt atcgtattta tattgtcgat tttcagtgtt attagtaagt taggttggct
     4381 gaaaattaac tttatagaat cattgcctct aagagcgtat tctttagagt ggttcccagt
     4441 agcaattatt gcaacgatat taggctatct agtcggcata tttgtaaaac aagatccaat
     4501 taaatatcaa caggaataac gaaaaaagga agcatctcca atttaatcgt aggatgcttc
     4561 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
     4621 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
     4681 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
     4741 gtacgtttca atgtatcaag gtcaacaatg ccacgctcat tttctgttgc agtttcaatc
     4801 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
     4861 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
     4921 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagggcttga
     4981 ttaacatttt gaatcatacg aatttgtggc gcagtttgca acgctatttg tcttgaaagc
     5041 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
     5101 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
     5161 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
     5221 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
     5281 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
     5341 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
     5401 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
     5461 gtatctccaa taggaccaac atctttactt tgtacctcat ccaacatttg atgtgaaaat
     5521 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     5581 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     5641 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     5701 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     5761 ttgatgatgc ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     5821 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     5881 tcgtgtttgt tctaattttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     5941 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccctcaac
     6001 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     6061 tatatcatta atttgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     6121 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     6181 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     6241 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     6301 tatcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     6361 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     6421 cgataatttt tgatttagtt ctaactcttc aagttgatgg ctcgttacat ttgatgctgg
     6481 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     6541 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     6601 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     6661 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     6721 aataattact atagttaata ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     6781 taaaggtata aaaataagta tagagttttg aagtatggaa ggggtcttta ataatgtgga
     6841 cagttaccaa aattagagcc gattatgagg gatggtggtt attcagtgac tggccagaaa
     6901 acattgttga aaaatatcaa tatcaagatt ttgatgacat gtttaagcac tatcaacaat
     6961 tgattaatca atgtaaagtt cagttcgata actatgtcac aggcaaatat aatatttatg
     7021 cattttataa taattgtgac atgaactatt gcgaagattg tgaagaggat ttacaaatat
     7081 tttatagttt cattgtttta caaaataatg aagtatatta taaactacca ataattgatt
     7141 aaatgaatat tttttattat tgtattgcaa tttggtaata actgcactat aatacaagtt
     7201 aagcagatga ttattccata ttgcaaagaa tatttgagtg aaatttgcat gtattttttg
     7261 taatatgcga ataagcatat tgaatgaatt ttagtcttgg aggtttcaga ttatgaaaca
     7321 aggtacagtt aaatggttta acgctgaaaa aggattcggc tttatcgaag ttgaaggaga
     7381 aaatgacgta ttcgtacatt tttcagcaat taaccaagat ggttacaaat cattagaaga
     7441 aggtcaagct gttgagtttg aagtagttga aggcgaccgc ggtccacaag ctgcaaacgt
     7501 tgttaaacta taattcttag atttgaatca ttgattttaa ctaacacctt accaagaagt
     7561 aaggtgtttt tatatgctta ttacaaatac ttaacgcgat gagtttctca accaaattga
     7621 gaactctttt tttatatcac caacattgtt tgcaatagaa aaatatattt atataatata
     7681 attatggtgt acaacgacga ggtgatttta tgaaatatct tatccttagt ttagttgcta
     7741 atttattagt tttcggtgta ttatctgcaa ttggtttaaa tattaa
//
LOCUS       DATTQF010000112         7771 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.112, whole genome shotgun sequence.
ACCESSION   DATTQF010000112 DATTQF010000000
VERSION     DATTQF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.112"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..262)
                     /locus_tag="VB241_002059"
     CDS             complement(<1..262)
                     /locus_tag="VB241_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEP1245338.1"
                     /translation="MEFYQMPMFNKVLMNHLRLEKYQDLMLISQKDFEVGNSIYLNIL
                     VKDIEEKSQNISQKFIVSQLEEQPWNAKEMTIKDPDGNLITLT"
     gene            491..583
                     /locus_tag="VB241_002060"
     CDS             491..583
                     /locus_tag="VB241_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEP1245339.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(885..1706)
                     /locus_tag="VB241_002061"
     CDS             complement(885..1706)
                     /locus_tag="VB241_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1245340.1"
                     /translation="MDSLKVLTEVVKTQSFTKAAENLYTSQPSISRDIKRLELEYDVK
                     IFEFRHSKMSLTSDGEKLFQYALQRERLEQNLRHDLKMQPDIISGDLTIGSSYTYGEY
                     RLSQHLADLAKNYPELYIHVYLNNSDTVLQHIKNNTIDLGIIEKEVQSNIIHSRRIAQ
                     DEIVLIRKKSSSSNKSICFIRERGSGTRVYQENALSQMTSSSYLVEINNTNLIKNMVH
                     ADKGFSIVSKSTLTPYDLEKLEVTNLDIKRYFYLVIHKDKYIDTKLNEVIKNLIS"
     gene            1831..2826
                     /locus_tag="VB241_002062"
     CDS             1831..2826
                     /locus_tag="VB241_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEP1245341.1"
                     /translation="MKSLKSKAFIYGLLFTFIIAVISLLGSKLPFLDKIGALTIAILI
                     AILYRHFKGYPEAYRSGIAFSSKRLLKLAIILYGLKLNIYDVIGKGSDLVLIDIGVIL
                     FSIGLMLLLNKYIKGDKNLILLLGIGTGVCGAAAIAAVSPILKSREKDSAISIGIVAL
                     IGTIFSLAYTVIYSIFTISPEVFGVWSGTSLHEIAHVILASDFSGQEALSMGLLGKLG
                     RVFLLIPLSIILILIMRKKSHSENEKKRIDVPYFLLGFVMMALFHTYVSLPHMVMQII
                     DNITTICLLMAMVALGLNVSFKDLKDRAFKPLIAVIIVSICLSTVTFIVAKYFYS"
     gene            complement(3161..3736)
                     /locus_tag="VB241_002063"
     CDS             complement(3161..3736)
                     /locus_tag="VB241_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1245342.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEDEKSDK"
     gene            4003..6048
                     /gene="copB"
                     /locus_tag="VB241_002064"
     CDS             4003..6048
                     /gene="copB"
                     /locus_tag="VB241_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase CopB"
                     /protein_id="HEP1245343.1"
                     /translation="MSNNKKHQNHEHHSHQEHENHTSHGNHEHHHHGNFKSKFFISLI
                     FAIPIIILSPMMGVKLPFQISFTGSDWIVLILATILFFYGGKPFLSGAKDEISTKKPG
                     MMTLVALGISVAYIYSLYAFYMNNFSGSSTHTMDFFWELATLILIMLLGHWIEMNAVG
                     NAGNALKKMAELLPNTAVKLIDNNQREEVKISDIHIDDIVEVRAGESIPTDGIIVRGE
                     TSIDESLVTGESKKVHKTHNDDVIGGSINGSGTVQVKVTATGENGYLSQVMGLVNQAQ
                     NDKSKAELLSDKVAGYLFYFAVSIGLISFIVWMLIQNNVDFALERLVTVLVIACPHAL
                     GLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYIMMDKTGTLTEGNFSVNHYESF
                     TDELNNEEILSLFASLESNSNHPLATGIVDFAKGKNISYATPQEVNNIPGVGLEGTVD
                     NKKLKIVNVSYLDKSNFDYNKEQFTNLAQQGNSISYLIHDRQVIGIIAQGDKIKESSK
                     QMVSDLLSRNITPVMLTGDNKEVAQTVAEELGISDVHAQLMPEDKESIIQDYQSNGSK
                     IMMVGDGINDAPSLIRADIGMAIGAGTDVAIESGDVILVKSNPSDIINFLSLSKNTMK
                     KMVQNLWWGAGYNVIAVPLAAGILASIGLILSPAVGAILMSLSTIIVAINAFTLKLK"
     gene            6063..7496
                     /gene="mco"
                     /locus_tag="VB241_002065"
     CDS             6063..7496
                     /gene="mco"
                     /locus_tag="VB241_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-copper oxidase Mco"
                     /protein_id="HEP1245344.1"
                     /translation="MYKKMFTILITLFSIMFMVPNDTFAEGKHNMMDMKENDQKRNDM
                     MDMKSHDERKNLNSSQGKNEITFPKVLDPKKDNNGYKSYTLKAQKGKTEFYKGNFSNT
                     LGYNGNLLGPTLKLKKGDKVKIKLVNNLDENTTFHWHGLEIDGKVDGGPSQVIKPGKE
                     KTIKFEVKQEAATLWYHPHPSPNTAKQVYNGLSGLLYIEDDKKNNYPSNYGKNDLPII
                     IQDKTFVSKKLNYTKTKDEDGTQGDTVLVNGKVDPKLTTKEGKIRLRLLNGSNARDLN
                     LKLSNNQSFEYIASEGGHLEKTKKLKEINLAPSARKEIVIDLSKMKEEKVNLVDNDET
                     VILPIINKEKSTNKDTTPKVDKKIKLEGMDDNVTINGKKFDPNRIDFTQKVNRKETWE
                     IENVKDKMSGMKHPFHIHGTQFKVLSVDGKKPSEDMRGKKDVISLEPGQKAKIEVVFK
                     NTGTYMFHCHILEHEDNGMMGQIKVTK"
     gene            complement(7563..>7771)
                     /locus_tag="VB241_002066"
     CDS             complement(7563..>7771)
                     /locus_tag="VB241_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1245345.1"
                     /translation="VFGFMKAILGFTRMSVRGINKVKRELGFVLMALNIRKIAAQRAV
                     HYKINIKKADFHQIINRNQLFYIA"
BASE COUNT         2700 a         1149 c         1279 g         2643 t
ORIGIN      
        1 gagtaagtgt gattaagttt ccatctggat cttttattgt catttcttta gcgttccatg
       61 gttgttcttc taattggctt actataaatt tttgtgaaat gttttgtgat ttttcttcga
      121 tatcttttac taagatattt aagtatattg agtttccaac ttcaaaatct ttttgagaaa
      181 ttagcattaa gtcttgatat ttctctagtc tcaaatgatt cattagcact ttattaaaca
      241 ttggcatttg ataaaattcc attgttttca cctctttttt tatttattgt aatgtattac
      301 gttacgttaa tgtcaaaaag aggtggtttt tgaaacgagt ttcttcttgt cttgatacta
      361 taaaaaaaca aagttatata acataagacg acttatcgga agtagatatg cttgtatatt
      421 cacagatggt ataataatag agtcgcctat ctctcaggcg tcaattgacg aagagaggag
      481 gtgcattact ttgctaatca ttttcgttca catcatagca ccagtcatca gtggctgtgc
      541 tgttgcgtat tatacttatt ggcttagtaa acgcaacaaa taattaatag gcgactttat
      601 aagccagcat cgaaaaaatc cccacactac tgcaatagtg tggggattgg tgcattaatt
      661 gttaatcatt ttcgttatat agattataac actggttagt aaagaaatgc aaagtgatat
      721 gtacagtatc agttacaagg tatcgtttca atattttgac gctaattgtc aataatttaa
      781 aggataattt atgaatgtta tgtattgaga gtttatcctt tttttatata gattgtcatt
      841 agaacgtgca ttaaatttag aataaataat acaaagatca aaattcatga aatcaaattt
      901 ttaataactt cattaagctt cgtatcaata tacttatcct tatgtatgac taaatagaaa
      961 taacgtttga tgtctaagtt tgttacttct agcttttcta aatcataagg tgttaatgtt
     1021 gatttagaaa caatagaaaa gcctttatca gcgtggacca tatttttaat tagatttgtg
     1081 ttgttaattt ctactaaata agaggatgaa gtcatttgtg ataacgcatt ttcctgatag
     1141 actcttgtcc ctgatccacg ttctctgata aaacatattg acttatttga agatgatgat
     1201 tttttgcgaa ttaatactat ttcatcttga gcaatacgtc tactgtgtat aatattactt
     1261 tgaacctctt tttcgattat acctaaatcg atagtgttat tcttaatatg ttgtaatact
     1321 gtatcagaat tatttaaata cacatgaatg tataattcag gataattttt ggctaaatca
     1381 gcaagatgtt gtgacaatcg atattcgcca taagtgtaac tactccctat cgttaaatct
     1441 cctgaaatta tatcaggttg cattttcaaa tcgtgtctta agttttgttc taatcgctca
     1501 cgttgtaaag catattgaaa tagtttctcc ccatcacttg ttaaagacat tttagaatgt
     1561 ctaaattcga aaatttttac atcgtattcc agttctaatc gtttaatatc gcgacttatt
     1621 gatggctgtg aagtatataa attttctgca gctttagtaa aactttgcgt tttaactact
     1681 tcagttagaa cttttaatga atccattaac taggtctcct gacatttgaa tacctctatt
     1741 ataacaaaaa tgttatgagt aatatactta atatttattt tacgttataa ctatgcgatg
     1801 ctaagctttt gataaaggag gagttaaaaa gtgaagtcct taaaaagcaa agcctttatt
     1861 tatggtttat tattcacatt tatcatcgca gttataagtt tattaggctc aaagttacca
     1921 tttttagata agataggtgc tttaacaatt gctatactga ttgcgattct atatagacat
     1981 tttaagggtt accctgaagc atatcgttca ggaatcgcat tttcgtctaa acggttactg
     2041 aaattagcta ttatattata tggattaaag ttaaatattt atgatgtaat tggaaaaggt
     2101 agcgatttgg tattaatcga tattggagtc atccttttta gtatcggatt aatgttgtta
     2161 ctaaataaat atattaaagg agataaaaac ctcatcctat tattaggtat tggcactggg
     2221 gtgtgtggcg cggcagctat tgcagcagta tctccaatat taaaatctag agaaaaagat
     2281 tctgcgatta gtattggtat tgtcgcactt attggcacta tattttcgct tgcatatact
     2341 gtaatatatt ctatttttac aatatctcct gaagtatttg gggtctggtc aggtacaagt
     2401 ttacatgaaa ttgcacatgt aattcttgct tcagacttct caggccaaga ggctttgagt
     2461 atgggactgt taggaaaact gggacgtgtg tttttattaa tacctctgag tattatttta
     2521 attttaatta tgagaaaaaa atcgcatagt gagaatgaaa aaaagcgtat tgatgtacct
     2581 tactttttac ttggttttgt catgatggca ttattccata cttatgtatc cttgccacac
     2641 atggttatgc aaattataga taatatcact actatatgct tattgatggc aatggttgca
     2701 ttagggttaa atgtttcatt taaagactta aaagatcgtg ctttcaaacc attaatagct
     2761 gtcattattg tatctatttg tttatcaact gtgacattta ttgttgctaa gtacttttat
     2821 agttagacta taagtatatt aaaaaattga tatagattct cacaaacaaa cacaacacat
     2881 tgaaggcatt ccatattact acggtcaaaa aaagatcagc ctttttctta ctaggcctat
     2941 tcttctttat tattactgac atatcaagct ttacaatgca tattccgact taccttgaca
     3001 gtttaattat agtaaatttc aatatttcta ttaactatgg aacaatataa tttagggtac
     3061 attgtaatca tacaaatgaa gttgttgaat ttataatagc ttaaacgtat gattagtgtg
     3121 tattttttaa cttttattat tgttgaaagt taagttgtat ttacttatca ctcttttcat
     3181 cttctaattt attgagataa cgataaattg ttgtacgtga aacttgccat tgttcagcaa
     3241 ttgtcttaat tggcgtacca ttatcataaa gtgttttgag caattttaaa tcttgtttgt
     3301 ttaatttttc gggtcttcca ccataccgtc ctctagcacg tgctgcaatt ctaccagctg
     3361 aagaacgttc taaaattaaa ttacgttcaa attctgcaaa tgctgcgaat aaatgaaata
     3421 ataattgtcc ggtagaactt gatttatcca tcgttatatt ttcttctaaa ctatggaaac
     3481 ttacacctcg ttcattaagc tcattaacta atgtaattaa atcctccata ttacgtccta
     3541 gtctatctaa tctccaaaca acaatagtat ctccagacct tgcaaattca attgctttat
     3601 ctaaaccagg tcttttactt tttgaaccac tcatatgatc gttaaatatc ttttcacaac
     3661 catatgcatt taatcgatct tcttgtaaat ttaaattttg taatcctgtt gaaactcttg
     3721 cataacctat tttcaaaata caaacctcct atatccatca ttagtgtacc ataacttaaa
     3781 tacgggtact tttatgaaca tagatttttt attgggtttc tgaacataaa aataaatgta
     3841 atcctcactt atttcgatat aaaaaaagag tgtacagaaa atggttattt tctgtacact
     3901 acaaaataaa aaagtcaaat ggtatatacc cctatcccgt atatgttaca ctcaaattgt
     3961 cttattacta tctttaaaaa tataaaatta ggaggtttaa ttttgtcgaa taacaaaaaa
     4021 caccaaaatc atgagcatca tagtcatcaa gaacatgaaa atcacactag tcatggtaat
     4081 catgaacatc accatcatgg aaattttaaa agtaaattct ttatttcttt gatttttgcg
     4141 ataccgatta ttattttatc gcccatgatg ggagttaaat taccgtttca aatttcattt
     4201 acagggtcag attggattgt attaattctt gctacaattt tattctttta cggaggaaag
     4261 ccatttttat caggggctaa agatgaaatt tcaactaaaa aacctggaat gatgacgctt
     4321 gttgctctag gtatttcagt agcttatatt tatagtttat acgcctttta tatgaataac
     4381 ttcagtggtt catccacaca tacaatggac tttttctggg aattagcgac tttaatttta
     4441 atcatgttat taggtcattg gattgaaatg aacgcagtag gtaatgcagg caatgcactg
     4501 aagaaaatgg cagaactttt acctaatact gcagtgaagc tgattgataa caatcaacga
     4561 gaagaagtta aaatatcaga tattcatatt gacgatattg tggaagtcag agcaggcgaa
     4621 agtataccca cagacggaat cattgttcga ggagaaacat caattgacga atcattagtt
     4681 actggcgaat caaaaaaagt tcataaaact cataatgatg atgttattgg aggttcaatt
     4741 aatggttcag gtacagtaca agttaaagtg actgctacgg gtgaaaatgg ctacctatct
     4801 caagttatgg gtcttgttaa ccaagctcaa aacgataagt caaaagctga actcctctct
     4861 gataaagtcg caggttattt gttttacttt gcagtaagta ttggcctgat ttcatttatc
     4921 gtatggatgc ttattcaaaa caacgtcgat ttcgcacttg aacggctagt aaccgtatta
     4981 gtcattgctt gtccacatgc attaggcttg gcaatacctt tagttacagc acgttctacc
     5041 tctataggcg cacataatgg tttaatcatt aaaaacagag aatctgtaga aatcgcacaa
     5101 cacattgatt acatcatgat ggataaaact gggacactaa ctgaaggtaa cttttcagtg
     5161 aatcactatg aaagttttac agacgagtta aataatgaag aaatattgag tctatttgct
     5221 tcattagaaa gtaattctaa tcatccactt gccactggta ttgttgattt tgctaaaggc
     5281 aaaaatattt catatgctac tcctcaagaa gttaataata ttccaggtgt aggcttagaa
     5341 ggcacagttg ataataaaaa actcaaaata gtgaatgttt cttatctcga taaaagcaat
     5401 tttgattata ataaagaaca atttacgaat ctcgctcaac aagggaattc tattagttat
     5461 ctaattcatg accgacaagt tattggtatt atcgcacaag gcgataaaat taaagaaagt
     5521 tcaaaacaaa tggtttcgga tttattatcg agaaacatta cgccagtcat gcttacaggc
     5581 gataataaag aagtggcaca aacagtggct gaagaattag gtattagtga tgtccacgcg
     5641 caattgatgc ccgaagataa agaaagtatt atccaagatt atcaaagcaa tggtagcaaa
     5701 atcatgatgg ttggtgatgg tatcaatgat gcgccaagcc ttattagagc agatattggt
     5761 atggcaattg gcgcagggac agatgtagct attgaatcgg gggatgtcat actcgttaaa
     5821 agtaatcctt ctgatattat caattttttg tccctttcaa aaaatacgat gaaaaaaatg
     5881 gttcaaaatt tatggtgggg cgcaggatat aacgtgattg cagttcctct tgcagctggt
     5941 atattagctt ctattggttt aatattatca cctgctgtgg gtgcaatact tatgtcttta
     6001 agtacgatca tcgtagcaat taatgcattt acattaaaat taaaataaaa gaggtaaata
     6061 taatgtataa aaaaatgttc acaattttaa ttacgttatt ttctataatg tttatggttc
     6121 ctaatgatac ttttgcagaa ggtaagcaca acatgatgga catgaaagaa aatgatcaaa
     6181 agcgaaatga tatgatggat atgaaaagtc atgacgaaag aaaaaatttg aattcttcac
     6241 aaggaaaaaa tgaaataaca tttcccaaag ttttagatcc taaaaaagat aacaatggtt
     6301 ataaaagtta tacattaaaa gcgcagaaag gaaagacaga attttacaaa ggtaatttct
     6361 ctaacacttt aggatacaat ggaaatttac ttgggccaac tttaaaatta aaaaaaggag
     6421 ataaggttaa aattaagtta gtcaacaact tagatgaaaa tacaacattt cattggcatg
     6481 ggttagaaat agatggaaaa gtggatggag gcccttctca agttataaaa ccaggaaaag
     6541 aaaaaacaat aaaatttgag gttaaacaag aggctgctac attatggtat catccccacc
     6601 cctccccaaa tacagctaaa caagtttata atggtttatc agggttatta tatatagagg
     6661 atgacaaaaa gaataattat cctagcaatt atgggaaaaa tgatttgcct ataataatcc
     6721 aagataaaac atttgtatct aaaaagttaa attatacaaa aacgaaagat gaagatggta
     6781 ctcaaggtga tacagtcctt gtgaatggga aagtagatcc taaattaaca acaaaagaag
     6841 gaaaaatacg tttgagactt ttaaatggct ccaatgctcg ggatttaaat cttaagctaa
     6901 gtaataatca aagttttgaa tatattgctt cagaaggcgg ccatttagaa aagactaaaa
     6961 agttaaaaga aattaattta gctccttcag caagaaaaga aatagtaata gatttatcta
     7021 aaatgaaaga agaaaaagtc aatcttgttg ataatgatga aacagtaatt ttaccaatta
     7081 ttaacaaaga aaaaagtact aacaaagaca ctactccaaa agtagataaa aaaatcaaat
     7141 tagaaggtat ggacgataat gttactatta atggcaaaaa attcgatcct aacagaatag
     7201 atttcacaca aaaggtaaac cgtaaagaaa cttgggaaat tgaaaacgtc aaagataaaa
     7261 tgagtggtat gaagcacccg ttccatatcc acggaacgca atttaaagtt ttatcagtgg
     7321 atgggaagaa accttcagaa gatatgagag gcaaaaaaga cgttatatct ctggaacctg
     7381 gacaaaaagc caaaatagaa gtggttttta aaaatactgg gacatacatg ttccattgtc
     7441 acatacttga gcatgaagat aatggaatga tgggacaaat aaaagtaaca aaataattta
     7501 tatcgtcgcg atcattttat catctccaaa tattttaggg atgaggctgg gacattaagt
     7561 tcttaggcaa tgtaaaaaag ctgatttcta ttaattattt gatggaaatc agcttttttg
     7621 atatttattt tataatgtac agctcgttga gctgctattt tccttatatt aagtgccatt
     7681 aatacaaaac ctagctctcg tttaacttta tttattcctc gaactgacat tcgagtgaaa
     7741 cccaaaatag ccttcataaa tccaaaaaca g
//
LOCUS       DATTQF010000113         7698 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.113, whole genome shotgun sequence.
ACCESSION   DATTQF010000113 DATTQF010000000
VERSION     DATTQF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.113"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(149..484)
                     /locus_tag="VB241_002067"
     CDS             complement(149..484)
                     /locus_tag="VB241_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245346.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(573..1832)
                     /locus_tag="VB241_002068"
     CDS             complement(573..1832)
                     /locus_tag="VB241_002068"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP1245347.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYRFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(2298..3158)
                     /locus_tag="VB241_002069"
     CDS             complement(2298..3158)
                     /locus_tag="VB241_002069"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP1245348.1"
                     /translation="MPLVSMKEVLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            3376..3897
                     /locus_tag="VB241_002070"
     CDS             3376..3897
                     /locus_tag="VB241_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HEP1245349.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     ALDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(4006..5616)
                     /locus_tag="VB241_002071"
     CDS             complement(4006..5616)
                     /locus_tag="VB241_002071"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP1245350.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(5952..6482)
                     /locus_tag="VB241_002072"
     CDS             complement(5952..6482)
                     /locus_tag="VB241_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP1245351.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(6594..7454)
                     /locus_tag="VB241_002073"
     CDS             complement(6594..7454)
                     /locus_tag="VB241_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245352.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT         2425 a         1461 c         1020 g         2792 t
ORIGIN      
        1 tttaatatgt tcaaaataaa atgctttaaa attttgctgc atctaatttt ttatattttt
       61 aaataaaaat aactattccc acatcatcgc aaataatgcg aagacttaga aatagttata
      121 atcaatgcta tgtttaaacg tcatgtaatc atttagcagt acgttgatct gttaaatcga
      181 catatgattg cgcccatgct tcaattggat gtaaagcctc tgctaacgct ttaccttttt
      241 cagttagcac ataaataatt tgtactggac tcgtagaaat gatttgcttc tcaactaact
      301 cccattgtgc aagctctgac aacttaagac ttaaagcacg tggtgttatt gttttcaaat
      361 ctcttttcat atcggaaaag tgtgctgaac agtcattaca tcttgagaga taattaatga
      421 ttaatccatt ccaacttcta ccaagtattt taaaagtttc ttcgagatac ggacatactt
      481 ccatcatctt cacctctcat caataaattt agaatgtaaa cgaacaccct tttttaatca
      541 gtataattat tataccacaa aacaagataa atctatacag tttccgtcca aatatctgca
      601 cctaaagctt ttaaatgttc tacaatatct gtataacctc tataaatatg tttaacattg
      661 taaattgtag ttacaccctc agcaattaaa ccagcaataa ttaaacaagc tcctgctctt
      721 aaatcgctag cataaacttc agcaccatgt aatgttgatg gttttatcgt tgctgtgcct
      781 tcgtcaactt caatatttgc acccatgcgc tttaattctt caacatgttt aaaacgctcc
      841 ggataaatcg tatcagttac aaatgaagga ccatttgcca taaataataa tggtgtaata
      901 ggctgttgca aatcagtagc aaaacctgga tatactagtg ttttaatatc aacaaatcga
      961 tatggcgcat tattattgat gcgaattctt tcgtctctta catcaacatt cacacctaat
     1021 tcactaaatt tagcagttaa tgtttctaca tgtttcggaa caatattatt taatataaca
     1081 ttttctccac atgctgcagc gatacacata tatgtgcctg cttcaattct atcaggtata
     1141 acttgatact cagaaccatg taattctttg acaccattga ttttaattgt tgatgtaccc
     1201 gctcccttaa tattagctcc catacttgtt aagaagttag caacatcaac tacttccggt
     1261 tctttagcag cattttcaat tacagtttgt cctgttgcat aaactgcagc tagcataatg
     1321 ttaattgttg cacctacgct aaccatatct agaaaaatat gtgcaccttt taactcttta
     1381 gcttcgattt tcattgatgt cgtacttgat tcatcaattt cagcacctaa agctttaaat
     1441 cctttaatat gttgatcaat tggacgtggc ccaagtggac atcctcccgg taaaccaatc
     1501 acacattttt taaatctacc taacatggca cccatcatat agtatgaagc acgtaatgat
     1561 tcaactttat tatttggtaa tgcagcattt tgtatttcag ttgtatcaac ttctaattcc
     1621 gtaccattta gtgatgcctt aatatttaaa tcttctaata aactcactaa agttttaaca
     1681 tcagagattt gcggtaaccc ttctaatttc acatgtcctt gagctaataa tgttgcagga
     1741 atgatagcta cagcactgtt tttcgcgcca ctgatattaa cttccccatt tagtgtgcgt
     1801 ccacctctta tttttattac ctcttgagcc atatcattta tctcctttgt ccattacttt
     1861 ttaatttttt gcattattag cattataaat taattttact ttatttgtaa aatagtttat
     1921 tttaatcgtt aaaatcttgt ctttctattt tcgattgatt aataacaaat cattcacctt
     1981 tactaatata tgctgttctt ccccacttga ccatggtcta aagtctgatt attcaactaa
     2041 atattgacgt tgttttgaca attatttagt tttaattcta cggttttcac gtaaactttt
     2101 ttcataaatt gctcattttc cttataatta tatcacaatc aattttgata tattttacca
     2161 tttttacgct ttcattttaa aatcaaactg ttttatttat tacttataca acataaaaaa
     2221 gaagcaaaca ccattttgtc tgcttctttt aaaattaata tccctacgtt attaataact
     2281 taaagactaa atattaatta tttagcgcgg tttgaagtac cgaactcttt aattttacct
     2341 ttaactgttt ctttgatagc ttcacgtgca ggtcctaagt atttacgagg atcgtaaact
     2401 tctttgtcgt tatttaaaac gtcacgaact gcttttgctg aagcgatttg gttttcagtg
     2461 tttacgttaa ttttagctgt accaaatgga attgcttttt ggatatcttt agtcgggata
     2521 ccagtaccac cgtgtaatac taatggtaaa cctgtagata aaccgatttc ttccatttct
     2581 ttaaatccta attttggttc acctttgtat ggaccatgaa ctgaacctaa tgctggcgct
     2641 aatgcatcaa taccagtttt ttcaactagt tcttgacatt ctttaggatc agcataaatg
     2701 atgccgtctg ctacaacatc atcttcttgt ccaccaacag tacctaattc agcttctaca
     2761 gaaacacctt tttcatgagc gtattcaaca acttttttag ttgttgctac gttttcttcg
     2821 aatgggctgt gtgaagcatc gatcattact gatgtgaaac cagcatcgat agcttcttta
     2881 catttttcaa agcttgaacc atggtctaaa tggattgcta caggaatagt gatgtttaag
     2941 tcatgcatta acccttcaac cattttaaca attgtgtaga aaccgctcat gtaacgagca
     3001 gcaccttcag aaacacctaa aattacaggt gcattttctt cttgtgacgc ttctaaaatt
     3061 gcttgagtga attctaggtt attaatattg tattgaccta ccgcataacc attttctttt
     3121 gcatcaatta acacttcttt cattgaaact aaaggcatga aagttcctcc ttgagtgctt
     3181 acgcacgtat ttttcaaagt aattatatca aaaaaatagc gtcaatgcat tgaaatactg
     3241 cagattaaat gtgtattttt tgaaaaatgc gatagttata ctaaatgcaa acgtatacat
     3301 ttttatacaa tttaagccgt tgcataatat gtagtcatta attacaatat tttataagta
     3361 gattggagga taaagatgtc aaaaatctta aacacacaac taataggtat ctttaatagg
     3421 ctagaaaaac aatctttaga aattcaaatg gccgcccaat gtttaattca agcaattggt
     3481 ggcgaaggtt atgtttatgt aaaaggttat gatgatttac agtttttcga aagtttcatt
     3541 ctacatagtg atgagcgttt aaaatctagc cgaaaacttg acgctattaa agactttaaa
     3601 gaaattgact caactgatcg tgtattatta ttcgcaccat tttataatga tcaagttgca
     3661 ttagatattc aaaaacttat cgccttagat atcgatgtgg tcttaataag caacaaacct
     3721 aaaacagatg atttccctga tcatctcgta cattttatcg atttatctac accaagacct
     3781 atagtctata ctgaagatta cgataagatt gtccagccac atgcgatggc attgaattat
     3841 gtatactatg acatttatac acaaatgatt gaaatgacaa gagacttaga attataaaag
     3901 tcactattgt tatttaatac cttgtcacta acataatatt aaaattataa gaaaaaccgg
     3961 tactttcaat gtttaacaat gaaagtaccg gttttattag caaatttatt tattttgttg
     4021 atattttaat gaagcttcaa tgaatgattt aaaaatcggg tgcggacgat ttggtctaga
     4081 taagaattct gggtggaatt gacaagcaat aaagaaatca tttgtcggaa tctctaccat
     4141 ttctactaaa cgtccatctg gacttgtacc agaaattacc ataccatttg cttctaattg
     4201 ttctctatag tcattattaa attcataacg atgacgatgt ctttcttcaa tttccgcttt
     4261 accgtaaaca tcttgtgcca atgtgccttc tttaattgaa catggatata agcctaagcg
     4321 taatgtacca cctaaatctt cgatatcttt ttgttctggt aataaatcta taattgggta
     4381 tggtgttgct gggtctaatt cagctgaatg tgcgccttca aggcctaata cgtttcttga
     4441 aaattcaact gttgcaagtt gcattcctaa acaaatacca aagaatggta cattgttttc
     4501 tctagcatac ttaattgcac taattttacc ttcacttgca cggaaaccaa atccacctgg
     4561 tactaaaata ccgtcgacat ctgcaaggta ttcggccgca ttttcatctg taacttcact
     4621 tgaatcaatc catctaatat caatatcttt agcgaaagga tatccggcat gttttaatga
     4681 ttcaactaca gataaatatg catcttgtaa gctaacatac ttaccaacta aaccaattgt
     4741 aattttacca tccaaattat taacgatatc taacaactgt ttccattcat caagctgtgt
     4801 ttcatatttc gcgtttaatt gtaaacgttt aataacgata tcgtccatat tttgttggct
     4861 taattgtaac ggaatttcgt ataaagaatc tgcatcacga cattcaataa cactttcttt
     4921 attaatgtca cagaataatg caattttatc ttttaaatct tgtgtcattt catattcagt
     4981 tcttacaacg attaagtctg gttgaatacc taagcctcgt aattctttaa cgctatgttg
     5041 tgttggcttc gttttcattt ctccagcagc tttaatataa ggcagtaatg tacagtgaac
     5101 atacataaca ttttctctac ctaaatcgct acgaatttga cgaatcgctt caataaacgg
     5161 taatgactca atatcacctg ttgtaccgcc aatttcagtg ataacaacgt ctgcattcgt
     5221 actttcccct gcaagtaata aacgttcttt aatttcattt gtgatatgcg gaataacttg
     5281 aactgttccg cctaagtaat caccacgacg ttcttttttc aatacgtgtg aatacacttt
     5341 accggctgtt acatttgaaa acttgtttaa attaatatca ataaatcttt cgtaatgtcc
     5401 taagtctagg tcagtttctg caccatcatc cgttacgaat acttcaccat gttgataagg
     5461 actcattgta cctgggtcaa catttaagta tggatcgaat ttttgaattg ttacatttag
     5521 acctctatct tttaataatc tacctagaga agatgctgta atacccttcc ctaatgatga
     5581 aactacgcca cctgttacaa aaataaattt tgtcatgtct tgacctccta attattaaag
     5641 ggaatgcttc ctcatcaatt gtaaaaatta cgtaaaacat aacatttgag taagctcatt
     5701 caagttgttc atttgtcgaa ttcgacaaaa ttaaattctg tttacttacc gttttcatac
     5761 tgctatgtca ctatgtatat catttacaac tttgattatt gcgttatcat ttcttgattt
     5821 actcaattaa ataaaaaaac gctccctctc acaaattttg taaggggagc gtataaattt
     5881 tatacacgtg tcctatttaa aggagcccga ataaaatttt atcatctttc aactaaaagt
     5941 caaacaaaaa attaatcgtt gaagtcttct tcgtcttcaa acacttcttc atcttcgtct
     6001 agttcatctt catcttcttc aatgactata tccgaatgat tgatttcttc ttcaacctgc
     6061 tcatcttctg gatcatttag ttcttcttga tcatctgttt gagctggaat atcatcgtcg
     6121 tcatccattt catcttcgcc caataatttt aagttttgat cttcttcatc atctgcatcc
     6181 agaatatcga atttttgaat agttggtgcg attttctctt caatatcatc taccgaatac
     6241 caatcacgta atccccataa attttctcca acatttaaaa aacgaccatc tgtgtttaaa
     6301 tctgtgtaaa attgtacaac gcgattttca atttcttcgt actcataatc acctaacgct
     6361 ctaaactcat cgatgatatc atataagttc atagtttcgc ctttatcatt caataatgta
     6421 taagccatat caataaatga tttttcatca accatttgtt ttgtataatc ttgaattttc
     6481 attgcccaat acttcctttc gaaggatata atctaaattt atacgtctga attcatttat
     6541 atatagtaac aaaactaaat tataaatgac aatatcatat ttcgcaatca ctgttagtaa
     6601 tttaaatatt tttcaaaaac gacataatca tcagtttgtt cgatttcaac gaaattacta
     6661 tttaaacata atggtttaaa atcattattc tctccaaaca gaatcatcgt aatcacatta
     6721 aggttgtcat agttttttct taagtaagta ggcaattgtc gaagcgcact tgaaccaata
     6781 ccattaaagc gatatgattt atccacttca atagcagcaa tgttcatgcg ttcaccaatc
     6841 tgctccaatg cgataaagcc aaccttttgg ccatcaatca ttaaattaat gatatgtaca
     6901 ttggtcactt ctgaacgact ttcttcttca agctttaatg acgaaacatg ttttgaatct
     6961 aagtccaagt aatatagtcg ttctttgcca attggtcctt cttctttagt cgctgtatgc
     7021 atgaatccag cacgttcgta taggttccaa gcattgtcat tttccgcatc tactactaga
     7081 tatagatgat taaaatcagg aaatacacct tgaacatatt gcggcaatga catcattatt
     7141 ttcgtgccat atccaaaacc ttgatatttt tcattaatcg ataatgaacg aatataaacg
     7201 acattttcag gtgtatcata accttcatgc tgatagtaac gatgtaatac aaaaaagccc
     7261 accacttcat ttttatcgtt tattgctacg ttagcaatcc tatcagcatc attaattgca
     7321 tcatcaataa cttctttagg taaagatgaa tatatttgtt gtctttcatt caagtcaaaa
     7381 tcattaattg ctgaacgata ttgttcatca aatgctttta aagtgatacc ttcaaaacaa
     7441 agttctttag ccatatattt aacccctatc tttttatata tgaagcatga atccccatat
     7501 tattttacct attatttcac tacgatacca ttatttgctc taagttaaat aaattactta
     7561 tttatatttt ttaaatttca aaattaatgt gctattcctc attacttatc attttaatca
     7621 gagacttaca aatacactgc gtacgaacta cttttaatat tgcgtcacta actaacaatc
     7681 ctatacaact tcaaaatt
//
LOCUS       DATTQF010000114         7546 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.114, whole genome shotgun sequence.
ACCESSION   DATTQF010000114 DATTQF010000000
VERSION     DATTQF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.114"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            68..964
                     /gene="xerC"
                     /locus_tag="VB241_002074"
     CDS             68..964
                     /gene="xerC"
                     /locus_tag="VB241_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEP1245353.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            961..1506
                     /gene="hslV"
                     /locus_tag="VB241_002075"
     CDS             961..1506
                     /gene="hslV"
                     /locus_tag="VB241_002075"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEP1245354.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1572..2975
                     /gene="hslU"
                     /locus_tag="VB241_002076"
     CDS             1572..2975
                     /gene="hslU"
                     /locus_tag="VB241_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEP1245355.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLAVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3000..3773
                     /gene="codY"
                     /locus_tag="VB241_002077"
     CDS             3000..3773
                     /gene="codY"
                     /locus_tag="VB241_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP1245356.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3824..3916
                     /locus_tag="VB241_002078"
     CDS             3824..3916
                     /locus_tag="VB241_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245357.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            4115..4882
                     /gene="rpsB"
                     /locus_tag="VB241_002079"
     CDS             4115..4882
                     /gene="rpsB"
                     /locus_tag="VB241_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP1245358.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4916..5029
                     /locus_tag="VB241_002080"
     CDS             4916..5029
                     /locus_tag="VB241_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245359.1"
                     /translation="MGDKILMLITFFKKKIKANYKYSIRVLAILPIIMLKL"
     gene            5064..5945
                     /locus_tag="VB241_002081"
     CDS             5064..5945
                     /locus_tag="VB241_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP1245360.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6082..6804
                     /locus_tag="VB241_002082"
     CDS             6082..6804
                     /locus_tag="VB241_002082"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP1245361.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6823..7377
                     /gene="frr"
                     /locus_tag="VB241_002083"
     CDS             6823..7377
                     /gene="frr"
                     /locus_tag="VB241_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP1245362.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         2862 a         1057 c         1495 g         2132 t
ORIGIN      
        1 gattatcaaa ttactgtaag ccctttgctg tatatgctac aattcttatt gatggagggt
       61 aaatgtattg aatcatattc aagaagcatt tttaaataca ttgaaagttg aacgaaattt
      121 ttcagaacac acattgaaat catatcaaga tgacttaatt cagtttaatc aatttttaga
      181 acaggaacat ttacagttga atacttttga atacagagat gctagaaatt atttgagcta
      241 tttatattca aatcatttga aaagaacatc tgtttctcgt aaaatctcaa cgttaagaac
      301 tttctatgaa tattggatga cgcttgatga gaacattatt aatccatttg ttcaattagt
      361 acatccgaaa aaagaaaaat atcttccgca attcttttac gaagaagaaa tggaagcgtt
      421 atttaaaact gtagaagagg acacttcaaa aagtttacgg gatcgagtta ttcttgaatt
      481 gttatatgct acaggtatcc gtgtttcgga attagttaat attaaaaaac aagatataga
      541 tttttacgcg aatggtgtta ccgtattagg aaaagggagc aaagagcgct ttgtaccgtt
      601 tggtgcttat tgtagacaaa gcatcgaaaa ttatttagaa catttcaaac caattcagtc
      661 atgcaatcat gattttctta ttgtaaatat gaagggtgaa gcaatcactg aacgcggtgt
      721 acgatatgtt ttaaatgata ttgttaaacg aacagcaggc gtaagtgaga ttcatcccca
      781 caagctcaga catacatttg caacgcattt attgaatcaa ggtgcagacc taagaacagt
      841 acaatcgtta ttaggtcatg ttaatttgtc aacaactggt aaatatacac acgtatctaa
      901 ccaacaatta agaaaagtgt atctaaatgc acatcctcga gcgaaaaagg agaatgaaac
      961 atgagtaata caacattaca tgcaacaaca atttatgctg taagacataa tgggaaagca
     1021 gctatggctg gagatgggca agtaacgctt ggtcaacaag tcatcatgaa acaaacggca
     1081 agaaaagtgc gtcgtttata tgaaggtaaa gtgttagctg gtttcgcagg tagtgtagca
     1141 gatgcgttta cgttatttga aaaattcgaa acaaaattac aacagtttag tggtaactta
     1201 gaaagagctg ctgttgaatt ggcacaagaa tggcgaggcg ataaacaatt acgtcaatta
     1261 gaagctatgc taattgtaat ggataaagat gctattttag ttgtcagtgg aactggcgaa
     1321 gttattgccc cagatgatga ccttatcgct attggatcag gaggcaacta tgcattaagc
     1381 gcaggacgtg cattgaaacg ccatgcatcg catttgtctg ctgaagaaat ggcatatgag
     1441 agcttgaaag tagcggctga tatttgtgtc tttaccaacg ataatattgt tgtcgaaaca
     1501 ctataataat cagagcatga taaataatta tgagcaatta attttagtta aaagacggag
     1561 gaatgaaatt aatggataca gctggaataa gattaactcc aaaagaaatc gtatctaaat
     1621 taaatgaata catcgttgga caaaatgatg ctaaacgtaa agtggcaatt gccctacgta
     1681 atcgatacag aagaagttta ttggatgagg aatcaaagca agaaatttca cctaaaaata
     1741 ttttgatgat tggaccaacc ggtgttggta aaactgaaat tgcaagaaga atggccaaag
     1801 ttgtcggcgc accatttata aaagtagaag ctactaaatt tactgaagta ggttatgtag
     1861 gacgagatgt tgaaagtatg gttagagatc ttgttgatgt ttcagtaaga ttagtcaagg
     1921 cgcagaaaaa atcattggta caagatgaag caacagctaa ggccaatgaa aaacttgtta
     1981 agttattagt tccaagtatg aaaaagaaag cgtctcaaac gaataatcct ttagagtcac
     2041 ttttcggagg tgcaattcca aatttcggac aaaataacga agatgaagaa gaaccaccta
     2101 ctgaggaaat taaaacaaaa cgttctgaaa ttaagagaca attagaagaa ggaaaacttg
     2161 aaaaagaaaa ggtaagaatt aaagtcgaac aagatcctgg tgctttaggt atgctaggta
     2221 caaatcaaaa tcagcaaatg caagagatga tgaatcaatt aatgcctaaa aagaaagttg
     2281 agcgagaagt tgctgttgag acggcaagga aaatcttagc tgatagttat gcggatgaac
     2341 taattgatca agaaagcgct aaccaagaag cacttgaatt agcagaacaa atgggtatca
     2401 tctttataga tgaaatcgac aaagttgcga cgaataatca taatagtggt caagatgtct
     2461 caagacaagg tgtgcaaaga gatattttac ctatacttga aggtagcgtt attcaaacca
     2521 aatatggtac tgtgaatact gaacatatgc tgtttatagg tgctggagct ttccatgtat
     2581 ctaagccgag tgacttgata ccagaattgc aaggtcgttt tccgattaga gttgaacttg
     2641 atagtttagc ggtagaagat tttgtaagaa ttttgacaga accaaaattg tcattaatta
     2701 aacaatatga agcattgctt caaacagaag aagttactgt aaactttacc gatgaagcaa
     2761 ttactcgttt agctgagatt gcttatcaag taaatcaaga tacagacaac attggtgcac
     2821 gtcgacttca tacaatttta gaaaagatgt tagaagattt atcattcgaa gcaccaagta
     2881 tgccgaatgc agttgtagat attaccccac aatatgttga tgataaatta aaatcaattt
     2941 caacaaataa agatttaagt gcatttattc tataaaaata tacaaaagga gaaaaattca
     3001 tgagcttatt atctaaaacg agagagttaa acacgttact tcaaaaacac aaaggtattg
     3061 cggttgattt taaagatgta gcacaaacga ttagtagcgt aactgtaaca aatgtattta
     3121 ttgtatcgcg tcgaggtaaa attttaggat cgagtctaaa tgaattatta aaaagtcaaa
     3181 gaattattca aatgttggaa gaaagacata ttccaagtga atatacagaa cgattaatgg
     3241 aagttaaaca aacagaatca aatattgata tcgacaatgt attaacagtt ttcccacctg
     3301 aaaacagaga attattcata gatagtcgta caactatctt cccaatttta ggcggaggag
     3361 aaagattagg tacattagta cttggtcgag tacacgatga ctttaatgaa aatgatttgg
     3421 tactaggtga atatgctgct acagttattg gtatggaaat cttacgtgag aagcatagtg
     3481 aagtagaaaa agaagcgcgc gataaagctg ctattacaat ggcaattaat tcattatctt
     3541 attctgaaaa agaagcaatt gaacatatct ttgaagaact tggcggtaca gaaggcctat
     3601 taatcgcatc aaaagttgca gatagagttg gtattactag atctgtaatt gtaaatgcac
     3661 tacgtaaatt agaaagtgct ggtgtaattg aatcacgttc tttaggaatg aaaggtactt
     3721 tcattaaagt taaaaaagaa aaattcttag atgaattaga aaaaagtaag taaaacgatg
     3781 agtctgggac ataattcaat gtcctagtcg taacgatgtt atcttggcag tagtcaacga
     3841 aatgactttg atgattatgt tgaagtcatt tctttatttt tgtctaattt tcaatcaaat
     3901 tgatattccg cattaacatg atagaattac ctttaaattt atgaaaaaat gcttgaaaaa
     3961 catttgcttt agacacggca ttatgttata tttattttcg gctataaagc caatacacac
     4021 atatcaaacg tgattgtgta aagggtgcaa tatttgtata ttgtctttac aatagtttgt
     4081 tatggaggta attaaccaat aggaggaatt tataatggca gtaatttcaa tgaaacaatt
     4141 actagaagcg ggtgttcact tcggtcacca aacacgtcgt tggaacccaa aaatgaaaaa
     4201 atatatcttc actgagagaa atggtattta tatcatcgac ttacaaaaaa cagtgaaaaa
     4261 agtagacgag gcatacaact tcttgaaaca agtttcagaa gatggtggac aagtcttatt
     4321 cgtaggaact aaaaaacaag cacaagaatc agttaaatct gaagcagaac gtgctggtca
     4381 attctacatt aaccaaagat ggttaggtgg attattaact aactataaaa cgatctcaaa
     4441 acgaatcaaa cgtatttctg aaattgaaaa aatggaagaa gatggtttat tcgaagtatt
     4501 acctaaaaaa gaagtagtag aacttaaaaa agaatacgac cgtttaatca aattcttagg
     4561 cggaattcgt gatatgaaat caatgcctca agcattattc gtagttgacc cacgtaaaga
     4621 gcgtaatgca attgctgaag ctcgtaaatt aaatattcct atcgtaggta tcgttgacac
     4681 taactgtgat cctgacgaaa ttgactacgt tatcccagca aacgacgatg ctatccgtgc
     4741 ggttaaatta ttaactgcta aaatggcaga tgcaatctta gaaggtcaac aaggcgtttc
     4801 taatgaagaa gtagctgcag aacaaaacat cgatttagat gaaaaagaaa aatcagaaga
     4861 aacagaagca actgaagaat aatcaactgt tgaatctgac ttagatatag tttaaatggg
     4921 tgataagata ttaatgctta tcaccttttt taaaaagaaa atcaaggcaa attacaaata
     4981 ttcaattaga gtattggcaa tcttgcctat aataatgcta aaattataat atataaaatg
     5041 ataacttatt ggaggaataa tgaatggcaa ctatttcagc aaaacttgtt aaagaattac
     5101 gtgaaaaaac tggcgcgggt atgatggatt gtaaaaaagc gctaactgaa actgatggtg
     5161 acatcgataa agcgattgat tacctacgtg aaaaaggtat tgctaaagca gctaaaaaag
     5221 cagaccgtat tgcggctgaa ggtttagtac atgtagaaac taaaggtaac gacgcagtta
     5281 tcgttgaaat caactctgaa acagactttg ttgctcgtaa cgaaggtttc caagagttag
     5341 ttaaagaaat cgctaatcaa gtattagata caaaagctga aactgttgaa gctttaatgg
     5401 aaacaacttt accaaatggt aaatcagttg atgaaagaat taaagaagca atttcaacaa
     5461 tcggtgaaaa attaagtgtt cgtcgttttg ctatcagaac taaaactgat aacgatgctt
     5521 tcggcgctta cttacacatg ggtggacgca ttggtgtatt aacagttgtt gaaggttcaa
     5581 ctgacgaaga agcagcaaga gacgttgcta tgcatatcgc tgcaatcaac cctaaatatg
     5641 tttcttctga acaagttagc gaagaagaaa tcaaccacga aagagaagtt ttaaaacaac
     5701 aagcattaaa tgaaggtaaa ccagaaaaca tcgttgaaaa aatggtggaa ggacgtttac
     5761 gtaaatactt acaagaaatt tgtgctgtag atcaagactt cgttaaaaac cctgatgtaa
     5821 cagttgaagc tttcttaaaa acaaaaggtg gaaaacttgt tgacttcgta cgctatgaag
     5881 taggcgaagg tatggaaaaa cgcgaagaaa actttgcgga tgaagttaaa ggacaaatga
     5941 aataatctgt cataaagtaa aacaaggaag aagacacctt taatgttgct ttattaaaat
     6001 gtaaatcatt ctaataaaac gacaactgtg tcttctttac ttgtatatgt tacatatatt
     6061 cacgatagag aggataagaa aatggctcaa atttctaaat ataaacgtgt agttttgaaa
     6121 ctaagtggtg aagcgttagc tggagaaaaa ggatttggca taaatccagt aattattaaa
     6181 agtgttgctg agcaagtggc tgaagttgct aaaatggact gtgaaatcgc agtaatcgtt
     6241 ggtggcggaa acatttggag aggtaaaaca ggtagtgact taggtatgga ccgtggaact
     6301 gctgattaca tgggtatgct tgcaactgta atgaatgcct tagcattaca agatagttta
     6361 gaacaattgg attgtgatac acgagtatta acatctattg aaatgaagca agtggctgaa
     6421 ccttatattc gtcgtcgtgc aattagacac ttagaaaaga aacgtgtagt tatttttgct
     6481 gcaggtattg gaaacccata cttctctaca gatactacag cggcattacg tgctgcagaa
     6541 gttgaagcag atgttatttt aatgggcaaa aataatgtag atggtgtata ttctgcagat
     6601 cctaaagtaa acaaagatgc ggttaaatat gaacatttaa cgcatattca aatgcttcaa
     6661 gaaggtttac aagtaatgga ttcaacagca tcctcattct gtatggataa taacattccg
     6721 ttaactgttt tctctattat ggaagaagga aatattaaac gtgctgttat gggtgaaaag
     6781 ataggtacgt taattacaaa ataaatttag aggtgtaaaa taatgagtga cattattaat
     6841 gaaactaaat caagaatgca aaaatcaatc gaaagcttat cacgtgaatt agcgaacatc
     6901 agtgcaggaa gagctaattc aaatttatta aatggcgtaa cagttgatta ctatggtgca
     6961 ccaacacctg tacaacaatt agcaagcatc aatgttccag aagcacgttt acttgttatt
     7021 tctccatacg acaaaacttc tgtagctgac atcgaaaaag cgataatagc agctaactta
     7081 ggtgtcaacc caacaagtga tggtgaagtg atacgtattg ctgtacctgc cttaacagaa
     7141 gaacgtagaa aagagcgcgt taaagatgtt aagaaaattg gtgaagaagc taaagtatct
     7201 gttcgaaata ttcgtcgtga tatgaatgat cagttgaaaa aagatgaaaa aaatggcgac
     7261 attactgaag atgagttgag aagtggtact gaagatgttc agaaagcaac agacaattca
     7321 ataaaagaaa ttgatcaaat gattgctgat aaagaaaaag atattatgtc agtataaaac
     7381 taatatacaa tgacatatta aaatgccagt attaaacgat aatgtaacat ttaaaatggg
     7441 catgtttaat taaatcaaag atgcatgtga taatttaaat tcacaatgag cataaaaatg
     7501 gtgtttaaac aagttaatta aacatatact ttataaataa taggca
//
LOCUS       DATTQF010000115         7538 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.115, whole genome shotgun sequence.
ACCESSION   DATTQF010000115 DATTQF010000000
VERSION     DATTQF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.115"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            11..160
                     /locus_tag="VB241_002084"
     CDS             11..160
                     /locus_tag="VB241_002084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245363.1"
                     /translation="MTCIHNLNSNNELVGIHFASDVKDDDNRNAYGVYFTPEIKKFIA
                     ENIDK"
     gene            218..937
                     /gene="splC"
                     /locus_tag="VB241_002085"
     CDS             218..937
                     /gene="splC"
                     /locus_tag="VB241_002085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HEP1245364.1"
                     /translation="MNKNLVIKSMAALAILTSVTGISAAVVEETQQIAKAEKHVTQVK
                     DTNVFPYHGVVAFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGNEDISVMNIEEQAVERGSNGFNFNDNVQAFKFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKVGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            1061..1777
                     /locus_tag="VB241_002086"
     CDS             1061..1777
                     /locus_tag="VB241_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEP1245365.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVSTTMVEGIQQTAKAEHNVTKIT
                     NTSISPYKGILRVGAATGFVVGKNTILTNRHVVKDVQVGSTVLAHPNGENDTGGYYKV
                     KKVIPYAGSADLAIVEVEEDSVYPKNKKFSENTEILTLASEAKANERVAIVGYPAPYK
                     NKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVGVLYSGDQIGHSKKE
                     SYSVYLTPEIKKFIADNTEK"
     gene            1944..2659
                     /locus_tag="VB241_002087"
                     /pseudo
     CDS             1944..2659
                     /locus_tag="VB241_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            2823..3539
                     /gene="splE"
                     /locus_tag="VB241_002088"
     CDS             2823..3539
                     /gene="splE"
                     /locus_tag="VB241_002088"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HEP1245366.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGIVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYAGKEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIISDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPANKSTK
                     GYGVYFSPEIKKFIADNLDK"
     gene            complement(3488..3605)
                     /locus_tag="VB241_002089"
                     /pseudo
     CDS             complement(3488..3605)
                     /locus_tag="VB241_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3709..4428
                     /gene="splF"
                     /locus_tag="VB241_002090"
     CDS             3709..4428
                     /gene="splF"
                     /locus_tag="VB241_002090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HEP1245367.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAENSVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKYEAIGVIYAGNKPSGEST
                     RAFAVYFSPEIKKFIADNLDK"
     gene            4493..4627
                     /locus_tag="VB241_002091"
                     /pseudo
     CDS             4493..4627
                     /locus_tag="VB241_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4791..6347
                     /locus_tag="VB241_002092"
     CDS             4791..6347
                     /locus_tag="VB241_002092"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP1245368.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     CFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKVELIDQVGYFIEPQDLFSAMIH
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGIIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKKTIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            6340..7494
                     /locus_tag="VB241_002093"
     CDS             6340..7494
                     /locus_tag="VB241_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP1245369.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGDLIKVNSGKDYKHLEKG
                     DIPVYGTGGYMTSVSEPLSEIDAVGIGRKGTINKPYLLEAPFWTVDTLFYCTPKKETD
                     ILFILSLFRKINWKVYDESTGVPSLSKQTINKINRFVPSNKEQQKIGEFFIKLDRQIE
                     LEEQKLELLQQQKKGYMQKIFSQELRFKDENGNDYPNWEEKKIEDIASQVYGGGTPNT
                     KIKEFWNGDIPWIQSSDVKVNDLILRQCNKFISKNSIELSSAKLIPANSIAIVTRVGV
                     GKLCLVEFDYATSQDFLSLSSLKYDKLYSLYSLLYTMKKISANLQGTSIKGITKKELL
                     DSIIKIPHNLEEQQKIGDLFYKIDKYISFNKCKIEILKSLKQGLLQKIFI"
BASE COUNT         2939 a         1151 c         1340 g         2108 t
ORIGIN      
        1 agggagaatt gtgacatgca tacacaacct aaatagcaac aacgaactag taggtattca
       61 ttttgcttca gatgtgaaag atgatgacaa tagaaatgct tatggcgtct acttcacacc
      121 agagattaaa aaattcattg cagaaaatat agataaataa tcaaattgtt ttaaaacgag
      181 cgttgcaata tatctcaaat tgtaaaggag cttgaaaatg aataaaaatt tagtcattaa
      241 aagcatggca gcattagcca ttttaacatc agtaactgga ataagtgctg cagtcgttga
      301 agagacacaa caaatagcaa aagcagagaa gcatgttacg caagttaaag atacaaatgt
      361 ttttccgtat catggcgtcg ttgcatttaa agatgcgaca ggctttgtaa tcggtaaaaa
      421 tacaattatc actaataaac atgtatcaaa ggattataaa gttggcgata gaattactgc
      481 tcatccaaat ggtgacaaag gaaatggtgg tatttataaa attaaaagta tttccgatta
      541 tccaggcaat gaagatattt ccgtcatgaa tatagaagaa caagcggttg aacgtggatc
      601 caatggcttt aattttaatg ataatgttca agcatttaaa tttgcaaaag atgctaaagt
      661 tgatgacaaa attaaagtta tcggttaccc gttacctgca caaaacagtt ttaaacaatt
      721 tgaatctaca ggaactataa aacgaattaa agacaatatt ttaaattttg atgcatatat
      781 cgaaccaggt aattcaggat cacccgttct aaattctaac aatgaagtca taggtgttgt
      841 atatggcggc attggaaaag ttggttctga atacaatggt gctgtatatt ttacgcctca
      901 aattaaagat tttattcaaa agcacattga acaataaaca tatttaaata tacaccatga
      961 gcatgtgttc aataatttta atgaaaaaca tcggtcgaat ataacataaa aagcaaacgt
     1021 ctatatcaaa agcatcatga ataaacagag gagcacaaaa atgaataaaa atataatcat
     1081 caaaagtatt gcggcattga cgatattaac atcagtgact ggtgttagca caacaatggt
     1141 tgaagggatt caacaaacag ccaaagccga acataatgta acgaagataa caaatactag
     1201 tatttcgcca tacaaaggca tactaagagt aggcgcagct acaggatttg tagtaggtaa
     1261 aaacactatt ttaacaaata gacatgtcgt taaagatgtg caagtaggta gtacagtgct
     1321 tgcccatcca aatggcgaaa atgatactgg cggatactat aaagtcaaaa aagttatccc
     1381 atatgcaggc tcggcagatt tagctatcgt tgaagttgaa gaagattcag tttacccaaa
     1441 aaataaaaag ttcagtgaaa atactgaaat cctaacgttg gcatcggaag ctaaagcgaa
     1501 tgaaagagtt gcaatcgttg gctatccagc gccatataaa aataaacatc atatgtatca
     1561 atcaacagga acagtactat caattaatgg agataaatta gtatcagatg catttgctga
     1621 aggtggcaat tcagggtcac cggtctttaa caataaaaat gaagttgtgg gcgttttata
     1681 tagtggagac caaattggtc attctaaaaa agagtcatac agcgtatatt taacgcctga
     1741 aattaagaaa ttcatcgcag ataacacaga aaaataagcc ggaaagctca cttacatgcc
     1801 tcaacttaga ttacattttt aaacttaata actaattcaa ctatcatcat atctctgtga
     1861 ttctatttat tcgaaataat tttaaaaata aaacttcaaa aacctaacct tatattaata
     1921 cgaatactta gaggagcaca aaaatgaata aaaatataat catcaaaagt attgcggcat
     1981 tgacgatttt aacatcagtg actggcgtcg gcacaaccat tgttgaaggt attcaacaaa
     2041 cagcaaaagc cgaacataat gtgaagcaag tacaaaacac aaatatttct ccgtataaag
     2101 gaattgttag aattggatca gctacaggat ttgtggtagg aaaaaataca attttaacaa
     2161 ataagcatgt attacctggt gttcaagttg gtagcacaat tcttgctcat ccaaatggcg
     2221 aaaatgatac cggtggatac tataaagtaa aaaaatcatt ccatacgcag gatctgaaga
     2281 tttagcaata gttcaagttg aagaagattc tgtttatcca aaaaataaaa aattcagtga
     2341 aaatactgaa gtccttaaat ttgcgagtgg cgctcaagta aatgatcgtt tatcaatcgt
     2401 tggttaccct aatccatata aaaataagca tcatatgttt gaatcaacag gaaagatatt
     2461 atccatcgat ggtaatcaaa tgacttcaga tgcatttgct gaaggtggca attcaggatc
     2521 cccagtgttt aattcaaatc atgaggtcat tgcaattgca tatgctgtcg atgtcaaaaa
     2581 tgatgcaaca aaaaaatcat atttagttta tttcacaccc gagatcaaga aattcattgc
     2641 agataacaca gaaaaataaa cgaacataca aatccatcct tacattgata aataattttt
     2701 taaaataaac aacaaactca acaattcaaa tcatatctct gtgattctat ttattcgaaa
     2761 tgattcaaaa aaactcaaaa acctaacatt ataattataa gaatacttag aggagcacaa
     2821 aaatgaataa aaatataatc atcaaaagta tagcagcatt gacgatttta acatcagtga
     2881 ctggtgttgg cacaacaatg gttgaaggta ttcaacaaac agccaaagcc gaacataatg
     2941 tgaaactaat caaaaatact aatgtagcac catacaatgg tatcgtttcg ataggatctg
     3001 gaacaggttt cattgtcgga aaaaatacaa ttgttactaa caagcatgtc gttgcaggta
     3061 tggaaattgg cgcacatatt attgcgcatc ctaatggtga atataataat ggcggttttt
     3121 ataaagttaa aaaaattgtc cgttatgcag gtaaagaaga tattgccatt ctacatgtgg
     3181 aagataaagc tgttcatcca aaaaatagga attttaaaga ttatacaggt attttaaaaa
     3241 tagcatcaga agccaaagaa aatgaacgca tttcaattgt tggctatcca gaaccttata
     3301 taaataaatt tcaaatgtat gaatcaacag gaaaagtgct atcagtgaaa ggcaacatga
     3361 ttatttctga tgctttcgta gaaccaggca actcaggttc agctgtattt aatagtaaat
     3421 acgaagttgt aggtgttcac tttggtggaa acggccctgc aaataaaagt acaaaaggat
     3481 atggcgttta tttctctcct gaaattaaga aattcatcgc agataattta gataaataat
     3541 taagcagata aatcaatcct tacataagat agatgatttt taaaaattaa caacaaaatc
     3601 aacaattcaa atcatatctc cgtgattcta tttattcgaa atgattaaaa aaataaaact
     3661 tcaaaaacct aaccttatat taatacgaat aattatagga gcacaaaaat gaataaaaat
     3721 ataatcatca aaagtattgc agcattgacg attttaacat cagtgactgg tgttggcaca
     3781 acaatggttg agggtattca acaaacagcc aaagccgaaa atagtgtaaa acaaattaca
     3841 aatacaaatg ttgcaccata cagtggggtt acatggatgg gcgcaggaac aggatttgta
     3901 gttggaaatc atacaatcat tactaataaa catgttacct atcacatgaa agtcggtgat
     3961 gaaattaaag cacatcctaa tggtttttat aataatggtg gtggacttta taaagttact
     4021 aagattgtag attatcctgg taaagaagat attgcggttg tgcaagttga agaaaaatca
     4081 acacaaccaa aaggtagaaa attcaaagat ttcactagca aatttaatat agcatcagag
     4141 gctaaagaaa atgaacctat atcagtcatt ggttatccaa atcctaatgg aaataaacta
     4201 caaatgtatg aatcaactgg taaagtatta tcagtgaatg ggaatatagt gtcatcggat
     4261 gcaataatcc agcctggtag ctctggatca cctatattaa atagtaagta cgaagctatt
     4321 ggtgtaatct atgcaggtaa taagccatca ggtgaaagca caagagcatt cgctgtttat
     4381 ttctctcctg aaattaagaa attcattgca gataatttag ataaataatt aaaacttaga
     4441 cattcaccca atcctgacaa aatatactat aactaacatt tattaatata tattgcatta
     4501 tttaatatgc atcaaagcca atcaatgatt gattttcacc aactcaattg ttgattggtt
     4561 ttatttatgt atgaatgaac aactttttga catcattgag aatataaatg attttgaaag
     4621 catttgaaag ctacaacatt tccaaaaaaa ttttcaataa caattgcgcc gctaaaactc
     4681 aaaatttcca caaccaacat caaaattcta aacatcgcaa caaaaccaaa tgttataata
     4741 aatctattac acaaagagat aaattactta tgcaaaggcg gaggaatcac atgtctatta
     4801 ctgaaaaaca acgtcagcaa caagctgaat tacataaaaa attatggtcg attgcgaatg
     4861 atttaagagg gaacatggat gcgagtgaat tccgtaatta cattttaggc ttgattttct
     4921 attgcttctt atctgaaaaa gccgaacaag aatatgcaga tgccttgtca ggtgaagaca
     4981 tcacgtatca agaagcatgg gcagatgaag aataccgtga agacttaaaa gtagaattaa
     5041 ttgaccaagt cggttacttc attgagccac aagatttatt cagcgcgatg attcatgaaa
     5101 ttgaaacgca agatttcgat atcgaacatc tggcgacggc aattcgtaaa gttgaaacat
     5161 caacactagg tgaagaaagt gaaaatgact ttatcggact gttcagcgat atggacttaa
     5221 gttcaacgcg tttaggtaac aatgtcaaag aacgtactgc gttaatttcc aaagttatgg
     5281 ttaatcttga cgatttacca ttcgttcaca gtgatatgga aattgatatg ttaggtgatg
     5341 catatgaatt ccttatcggg cgctttgcgg cgacagcggg taaaaaagct ggcgagttct
     5401 atacaccaca acaagtatct aagatactgg caaagattgt cacagacggt aaagataaat
     5461 tacgtcacgt gtatgaccca acatgtggtt caggttcatt actgttgcgt gttggtaaag
     5521 agacacaagt atatcgttat ttcggacaag aacgtaacaa taccacatac aacttagcac
     5581 gcatgaacat gttgttacat gatgtacgtt atgagaactt tgatatccgt aatgatgata
     5641 cgttggaaaa tccagccttt ttaggcaata catttgatgc ggttattgcg aacccaccat
     5701 acagtgcgaa atggacagca gattcaaaat ttgaaaatga tgaacgtttt agtggttacg
     5761 gcaaacttgc gccaaaatcc aaagcagact ttgcctttat tcaacacatg gtacattact
     5821 tagacgatga aggtaccatg gccgttgtac tcccacatgg tgtcttattc cgtggtgccg
     5881 cagaaggtat cattcgtcgt tatttaattg aagaaaagaa ctacttagaa gccgtgattg
     5941 gtttgccagc gaatattttc tatgggacaa gtattccaac atgtatttta gtatttaaaa
     6001 aatgtcgcca acaagacgac aacgtattat ttatcgatgc atccaatgat tttgaaaaag
     6061 gaaaaaatca aaaccattta agcgatgccc aagtcgaacg tattatagac acatataagc
     6121 gtaaaaaaac gattgataaa tatagctaca gcgcgacatt acaagagatt gccgataacg
     6181 attacaactt aaacataccg cgatatgtcg atacattcga agaagaagcg ccaattgatt
     6241 tagatcaagt ccaacaagat ttgaaaaata tcgataaaga aatcgcagaa attgaacaag
     6301 aaatcaatgc atacctgaaa gaacttgggg tgttgaaaga tgagtaatac acaaacgaaa
     6361 aatgtgccag agttgagatt cccagggttt gaaggcgaat gggaagagaa aaagttaggg
     6421 gatcttataa aagttaattc tggaaaagat tataaacatt tggaaaaagg tgatatacca
     6481 gtctatggta ctggcggtta tatgacaagt gtttcagaac cactaagtga aattgatgct
     6541 gttggtattg ggagaaaagg gactataaac aaaccatatt tgcttgaggc gccgttttgg
     6601 acggtggata cattatttta ttgtacacct aaaaaagaaa cagacatact atttatatta
     6661 agtttattta gaaaaataaa ttggaaagta tacgatgaat caacaggtgt gccaagctta
     6721 agtaaacaaa ccattaataa aataaataga tttgtccctt caaataaaga gcagcaaaaa
     6781 ataggcgaat tcttcatcaa actcgaccga caaattgaat tagaagaaca aaaacttgaa
     6841 ttacttcaac aacagaaaaa aggctatatg cagaaaatct tctcacagga attgcgattc
     6901 aaggatgaga atggaaacga ttatccgaat tgggaagaga agaaaataga agatatagca
     6961 agccaagtat atggaggcgg aacaccaaat acaaagatta aagaattttg gaatggagat
     7021 attccatgga ttcaaagctc tgacgtaaaa gtaaatgatt tgattctacg acaatgtaat
     7081 aaatttattt ccaagaattc aattgagctt tcttctgcaa aacttattcc tgccaattca
     7141 attgcaatag ttacaagagt cggggttgga aaactgtgtt tggtagaatt tgattatgct
     7201 acaagtcaag attttttatc attaagtagt cttaaatatg acaaattata ctcattatat
     7261 tcattgctat atacaatgaa aaaaattagc gctaatctac aaggaacttc aattaaaggt
     7321 ataacaaaaa aagagttgtt agatagtata ataaagatac cccataatct agaagaacag
     7381 caaaaaatag gtgatctatt ttataaaatt gataaatata tcagttttaa taaatgtaaa
     7441 attgagatac ttaaaagtct caaacaagga ttacttcaaa aaatttttat ataaatgata
     7501 tagaatttaa aattttgtag aggtgaattg atggaaaa
//
LOCUS       DATTQF010000116         7336 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.116, whole genome shotgun sequence.
ACCESSION   DATTQF010000116 DATTQF010000000
VERSION     DATTQF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.116"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            67..1173
                     /gene="rpoD"
                     /locus_tag="VB241_002094"
     CDS             67..1173
                     /gene="rpoD"
                     /locus_tag="VB241_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP1245370.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1304..1981
                     /locus_tag="VB241_002095"
     CDS             1304..1981
                     /locus_tag="VB241_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP1245371.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHRQRLAQINDEI
                     AVIERVL"
     gene            1984..3084
                     /locus_tag="VB241_002096"
     CDS             1984..3084
                     /locus_tag="VB241_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP1245372.1"
                     /translation="MKIADLMTLLDHHVPFRTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPHGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDACQKSWAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAANIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFEYI"
     gene            3198..4544
                     /locus_tag="VB241_002097"
     CDS             3198..4544
                     /locus_tag="VB241_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP1245373.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPEQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIVFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            4554..5444
                     /locus_tag="VB241_002098"
     CDS             4554..5444
                     /locus_tag="VB241_002098"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEP1245374.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQHFDPELKNKVMQQ"
     gene            5570..6355
                     /locus_tag="VB241_002099"
     CDS             5570..6355
                     /locus_tag="VB241_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP1245375.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            6397..7260
                     /locus_tag="VB241_002100"
     CDS             6397..7260
                     /locus_tag="VB241_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP1245376.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            7247..>7336
                     /locus_tag="VB241_002101"
     CDS             7247..>7336
                     /locus_tag="VB241_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEP1245377.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVE"
BASE COUNT         2702 a          953 c         1374 g         2307 t
ORIGIN      
        1 tttagtttaa aatagcaata ttgaataaat ttcgaatgtt catatttaaa atcgggaggc
       61 cgtttcatgt ctgataacac agttaaaatt aaaaaacaaa caattgatcc gacattaaca
      121 ttagaagatg ttaagaagca attaattgaa aaaggtaaaa aagagggtca tttaagtcat
      181 gaagaaattg ctgaaaaact tcagaatttc gatatcgact ctgatcaaat ggatgatttc
      241 tttgatcaat taaatgataa tgatatttca ctagttaatg aaaaagatag ttcagatact
      301 gacgagaaac tgaatccaag tgatcttagt gcccctccag gtgttaaaat aaatgaccca
      361 gttcgtatgt accttaaaga aattgggcgt gttaacttat taagtgcaca agaagaaatc
      421 gaattagcca aacgtattga acaaggtgat gaagtagcaa aatcaagact tgcagaagcg
      481 aacttacgtt tagttgtaag tattgctaaa agatacgtag gtcgtggtat gttattcctt
      541 gatttaatcc aagaaggtaa tatgggtctt attaaagctg ttgaaaaatt tgactttaac
      601 aaaggattta agttttcaac atatgcaaca tggtggatta gacaagcaat cactcgtgca
      661 attgctgacc aagcacgtac aattcgtatc cctgtgcata tggtagaaac aattaataaa
      721 ttaattcgtg ttcaacgtca attattacag gacttaggtc gagatccagc accagaagaa
      781 attggtgaag aaatggattt accagcagaa aaagttcgtg aaattttaaa aattgcgcaa
      841 gaacctgttt cattagaaac accaattggt gaagaagatg atagtcattt aggggacttt
      901 attgaggatc aggaagcaca aagtccttca gatcatgctg cttatgaatt attaaaagag
      961 caattagaag atgtgcttga tacattaact gatagagaag aaaatgtatt acgattaaga
     1021 tttggtcttg atgacggcag aacaagaaca cttgaagaag ttggtaaagt tttcggtgtt
     1081 acacgtgaac gtattcgaca aatcgaagca aaagcactta gaaaattaag acatccaagt
     1141 cgtagtaaac gtttgaaaga ctttatggat taatttatat caattttaat gattgatacc
     1201 aagacatgaa taaacgctta tttattttta gattaagatt taacttgagt gaggttggta
     1261 cattgaataa tgttcaacct cttttttcat aaaggagaat aaaatgattt cgttaaataa
     1321 ccgattaacg acggtaagtc gatttttaaa acaaggtaca atcgcggata ttggctcaga
     1381 tcatgcttat ttgccaatct atgcaataca aaaccattta tgcgaatgcg gtattgctgg
     1441 agaagttatt caaggccctt ttcaagctgc tgtgaaaaat gtagctgcaa accaattagt
     1501 agatagaatt gatgtacgtt taggggatgg cttaagtgtt attcatcctg aggatgtaat
     1561 cgataatatc acaatttgtg gtatgggcgg accacttatc gctaaaattt taaaagatgg
     1621 gcaagataag ttaagtcaac atccaaggtt gattttacaa agtaatatcc aaactgaaaa
     1681 tttaagacaa acattacaac aattgaatta tgaaataatt gatgaaataa taatggaaga
     1741 aaaaggccat atttatgaaa ttgttgtagc agaatattct acgcaattaa tagagctatc
     1801 atctgatgaa ttgaaattcg gaccaaaatt gctgaataac aaaaatgaat attttataaa
     1861 aaaatggcaa agagagttag aagcacttta tcatattaaa tcgaaactca atactgaaca
     1921 acatcgtcaa cgtttagctc aaattaatga cgaaattgct gtaattgaaa gggtgttata
     1981 gtaatgaaaa tagctgattt aatgacattg ttagatcatc atgtgccatt tagaactgct
     2041 gaatcttggg ataatgtagg attgttaata ggtgatgaag atgttgaagt tactggtgtt
     2101 ttaacagcat tagactgtac gttggaagta gtaaatgaag caatcgaaaa aggttataat
     2161 actattatta gtcatcatcc tctaatcttt aaaggtgtaa catcattaaa agctaatggt
     2221 tatggtttga ttattagaaa actaattcaa catgacatta atttaatagc gatgcataca
     2281 aatttagatg taaatcctca tggtgtcaat atgatgttgg cgaaggcgat gggattgaag
     2341 aacatttcaa taataaataa tcaacaagat gtatactata aagttcaaac atatatacct
     2401 aaggataatg ttggaccatt taaagataag cttagtgaaa atggattagc acaagaaggt
     2461 aattatgaat attgtttctt tgaaagtgaa ggaagagggc aattcaaacc agttggtgaa
     2521 gctaatccaa caataggaca aattgataaa attgaatatg tagatgaagt taaaattgaa
     2581 tttatgatag atgcatgtca aaagtcatgg gctgagcaat taattaaaca ataccatcca
     2641 tatgaaacac cggtatttga ttttattgag ataaaacaaa catctcttta tggacttggc
     2701 gttatggcag aagtggataa tcaaatgaca ttggaagatt tcgcagctaa tattaaatct
     2761 aaattaaata tcccaagtgt ccgttttgtt ggtgagtcta atcagaaaat taaacgtatt
     2821 gcaattattg gtggttcagg tattggatat gaatatcaag ctgttcaaca aggcgcagat
     2881 gtctttgtta caggtgatat taaacatcat gatgccttag atgctaaaat tcatggtgtg
     2941 aacttaattg atattaatca ttacagcgaa tatgtgatga aagaaggttt aaaaacgtta
     3001 ctaatgaatt ggttcaatac agaaaaaata aatatagatg ttgaagcatc tacaattaat
     3061 acagatccat ttgaatatat ttaaactaaa atctcgggat ttctaaattt tgaaatttcg
     3121 aggttttaat atttttattt aaaatagcac atttatactt tataatagta aagatgaaca
     3181 tataaggagg ccaaatcatg gcaaaacatc cattcgaaca atttaatcta gaatctagtt
     3241 taattgacgc tgtgaaagac cttaattttg aaaaaccaac tgaaattcag aatcgaatta
     3301 ttccaagaat actaaagaga acaaatttaa ttggtcaatc tcaaacgggt acagggaaat
     3361 ctcatgcatt tttattacca ttaatgcagt taattgatag tgaaataaaa gaaccacaag
     3421 caatcgtagt tgcaccaaca agagaacttg cacaacaact atacgatgca gcgaaccatt
     3481 taagccaatt taaagctggt gtttcagtta aagtttttat tggtggtaca gatatagaga
     3541 aagatagaca acgttgtaat gcacaacctc aattgattat aggtacccct actagaatta
     3601 atgatttagc taaaacggga catttacatg tgcacttagc atcatattta gttattgatg
     3661 aagcggatct tatgattgac ttaggattaa ttgaagatgt agattacatc gctgcaagat
     3721 tggaagataa tgcaaatatt gcggtgttta gtgctacaat tccagaacag ttacaaccat
     3781 ttttaaataa atatttaagt catccagaat atgtagctgt cgacagtaaa aaacaaaata
     3841 aaaagaacat cgaattcttt ttaataccta ctaaaggtgc agctaaagtt gaaaagactt
     3901 taaatttaat tgatatacta aatccatact tatgtattgt tttctgtaat agtagagata
     3961 acgcaaatga tttagcacgt tcactaaatg aagctggtat taaagttggt atgattcatg
     4021 gtggtttaac gccacgtgaa cgtaaacaac aaatgaaacg tatacgtaat ttagaattcc
     4081 aatacgttat agccagcgat ttagcatctc gtggtattga tattgaaggt gttagtcatg
     4141 tcatcaattt tgatgtgcca aatgatattg acttctttac acatagagtc ggccgaactg
     4201 gccgtgggaa ttataaaggt gtagcaatta cgctttatag tcctgatgaa gaacacaata
     4261 tttcattaat agaagatcgc ggttttgttt tcaatactgt tgatattaaa gatggtgagt
     4321 taaaagaagt taaagcgcac aatcagcgtc aagcaagaat gcgcaaagat gaccatttaa
     4381 ctaatcaagt gaagaacaaa gttcgaagta aaattaaaaa caaagttaaa ccaggttata
     4441 agaagaaatt taaacaagaa gttgaaaaaa tgaaacgtca agagcgtaaa caatttagta
     4501 agcagcaaaa tagacaaaaa cgtaagcaaa acaaaaaagg ttaggtgtgt tttatgttat
     4561 taggatcaca tgtttcaatg agtggtaaaa agatgttaga aggttctgct atagaagcgc
     4621 atgaatatgg tgaaacaaca tttatgattt atactggtgc acctcaaaac acgcgccgta
     4681 aaagtattga agatttaaat ataactaaag gtcatgaagt gatggaaaaa tatggcttat
     4741 ctaatattgt tgttcatgca ccatacatca ttaatattgc aaatacaacc aaaccagaaa
     4801 cgtttaatct tggtgtagat ttcttgcaac aagaaattga aagaacgcaa gctataggcg
     4861 ctaaagatat tgtattacat ccaggtgcac atgttggagc tggtgttgat gccggaatta
     4921 ataagattat tgaaggatta aatgaagttt taacgaatga caataatgta cgtattgcgc
     4981 ttgaaacgat ggcaggtaaa ggtacagaga ttggtcgctc gtttgaagag ttagcacgta
     5041 ttattgatgg agttcataat aacgaacgtt tatcagtatg ttttgataca tgtcatacgc
     5101 atgatgctgg atataatgtg aaagaggact ttgatggcgt attaaatgaa tttgataaaa
     5161 tcattggagt cgacaggatt aaagttgttc atgtcaatga ttctaaaaat gaccgtggcg
     5221 ctcaaaaaga ccgacatgaa aatattggct tcggttacat tggttttgat gcattaaatt
     5281 acattgtgca tcatgatagt tttaaagata ttccgaaaat cttagaaaca ccttatgttg
     5341 gtgaagataa gaaaaataaa aaaccgccat ataaacttga aattgaaatg ttaaaacaac
     5401 agcattttga cccagaatta aaaaataagg ttatgcaaca ataaaatgat aatgctctct
     5461 tcaagaaatg cgataaataa aacttgaagg gggcatatct ctttattttg tctaattttg
     5521 agtcgtaaac attactgttt acatatgtta ctaggagttg tatagttgaa tgcgaggtga
     5581 gaatacgatg acaccagtct ttgaattgaa aaatgtcaat tactactatg atcataaaaa
     5641 agtgttagaa aatataaaca ttaaaataaa taaaggtgaa tttttagcaa ttgttggacc
     5701 aaatggtgct ggtaaatcaa cattattgaa gttgattcta ggattattac ctttacaaag
     5761 tggtgagatt tttgttgaag gtattgattt taaaaataag aaaacatcaa ttaaattaag
     5821 ctatgtatca caaaaagcaa atgcctttaa ttcaggtttc ccagcaagtg ttaaagaagt
     5881 tgttttaagc ggattaacaa agacaaaacg tcttttccaa acatttaata gcaaagataa
     5941 tgaaaaagtg attaaagtac tagaaagact gaatataagt gatttaattc ataaaaatat
     6001 agcagaatta tcaggtggtc aacaacaacg tgtaatgatt gctcgagcat tgatttcaga
     6061 acctgcagta ttagtacttg atgaaccgac gaatggtatt gatgcaaaac atgtaagtga
     6121 attttataat actttagatc aattaaaaca agaaggtatc actattatct tagttactca
     6181 tgatattggt gttgtagcag atactgctac tgaagtagca tgtttaaata agcatttgca
     6241 tttccatggt acaactgatg agtttaaatc acttgatgaa gttgaaattt caaaaattta
     6301 tggacatcct gtacgttttg tcgatcatca gcataatcga gaatgttgta attaataaag
     6361 atttgagttt atttaaaatg gaaaggcgtg tcacaattga tagatgcatt attgaatttt
     6421 gattttatga ggtactcttt aattagtggt atcttgatag gttttattgc gcctctaatc
     6481 ggtgctttta tcgttgttag acgactatca cttatagctg atgctctaag ccatgtaact
     6541 ttaggtggta tatccttcgg tatgttttta cttactatta tgccaacact agtatttatt
     6601 aatccaatgt ggtttggaat cttattcgca atagtaggtg cgctactaat tgaaaaatta
     6661 agaacgtcat acactgctta ccaagaaatt gctattccaa ttataatgag tgctggtatc
     6721 gccttgagtg caatcttcat ttcattagct gatgggttca atcaagaaat tgttggattg
     6781 ctatttggct caataagcgc agtgaatata agtgatttaa caacgattat tgtcattgcc
     6841 atcatagttg ttctgtttat tacattgttt tataaagaat tatttatatt gtcatttgat
     6901 gaggaatata gtaaagtcat tggcattcct aaatggattc aatttttatt tattgttatt
     6961 gtagcgatgg ttatttctgc ctcaatgcgt gttgtaggga tactgttagt aagtgcgtta
     7021 atcacgttac caattgcaat aagtatgcga ataacaaaaa gttttaagca attgatatta
     7081 ctaagtgtat ttttaggtga attatcagta atattaggct tagtcctagc tttctatatg
     7141 gacatttctc cgggtggtgt aattgtagtt ctactagtta ttttacttat gattacaatg
     7201 gcttatcaga aaatgcgaat gaagtttaaa aagggagcta atatcaatga atacaaatga
     7261 tgctattaaa attttaaaag agaacggttt aaaatataca gataaacgta aagatatgtt
     7321 agatattttt gtcgaa
//
LOCUS       DATTQF010000117         7308 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.117, whole genome shotgun sequence.
ACCESSION   DATTQF010000117 DATTQF010000000
VERSION     DATTQF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.117"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(223..1509)
                     /locus_tag="VB241_002102"
     CDS             complement(223..1509)
                     /locus_tag="VB241_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1245378.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSRPIVTNALTHGLSLVGDLFVNQDDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVEAIKA
                     LANKGTKVIAVVDDAYYGLFYEDVYTQSLFTALTNLHSNAILPIRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEALRKHLIDKYSIG
                     VIALNATDIRIAFSCVEKDDIPHVFDSIAKAIDDLR"
     gene            1709..1807
                     /locus_tag="VB241_002103"
     CDS             1709..1807
                     /locus_tag="VB241_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245379.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2049..2225
                     /locus_tag="VB241_002104"
     CDS             2049..2225
                     /locus_tag="VB241_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245380.1"
                     /translation="MKHLTKIFVALAIILFIVGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNNK"
     gene            2483..2863
                     /locus_tag="VB241_002105"
     CDS             2483..2863
                     /locus_tag="VB241_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1245381.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            2860..3756
                     /gene="pmtA"
                     /locus_tag="VB241_002106"
     CDS             2860..3756
                     /gene="pmtA"
                     /locus_tag="VB241_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HEP1245382.1"
                     /translation="MNAIEVSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIFEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            3757..4437
                     /gene="pmtB"
                     /locus_tag="VB241_002107"
     CDS             3757..4437
                     /gene="pmtB"
                     /locus_tag="VB241_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HEP1245383.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLVGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            4434..5306
                     /gene="pmtC"
                     /locus_tag="VB241_002108"
     CDS             4434..5306
                     /gene="pmtC"
                     /locus_tag="VB241_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HEP1245384.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            5306..6046
                     /gene="pmtD"
                     /locus_tag="VB241_002109"
     CDS             5306..6046
                     /gene="pmtD"
                     /locus_tag="VB241_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HEP1245385.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGDKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(6110..6673)
                     /locus_tag="VB241_002110"
     CDS             complement(6110..6673)
                     /locus_tag="VB241_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1245386.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            6794..>7308
                     /locus_tag="VB241_002111"
     CDS             6794..>7308
                     /locus_tag="VB241_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245387.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEF"
BASE COUNT         2498 a          965 c         1236 g         2609 t
ORIGIN      
        1 atttgtagag atgtgatact tgaagtgatt tgccacacgt ttgataaatt tatctaaggt
       61 gtatcaagtt ttgttaggag agatgtataa aactaattag gtatagcgat taataagttg
      121 ctgaataaaa tatatcttat gatgctttga aagaaggaat aattttaaaa ataaaaaacc
      181 aataatccga gtcatacaca tcggattatt ggttgaagtt aattatctta agtcatcaat
      241 tgctttagca attgaatcaa atacatgtgg gatatcgtct ttttctacac aactaaacgc
      301 aatacgaata tctgtagcat taagtgcaat gacaccaata gaatacttgt caattaaatg
      361 cttgcgcaat gcttcagggt caacatcatg aacttttatt gccataaagt atccagagtt
      421 aaagtcatat gcttgccaat gtgaatgata ttgatcagca tagacaactt ccttagtgac
      481 ttcatagcgt tcttttaatg tttgaatatt ttgttcgatt tctttatcga attgtttatt
      541 attttttaat acatgcttaa cagcgctttg tgttggaagc ggtccactag aaatgttgct
      601 tcgtataaga ccttttactt tggcttctaa aacttcttta gttgtttgat cagacgttcc
      661 aaatgtcata aatccaacac ggaatcccca tgcgaaaaat tcttttgttg ccccatctaa
      721 acgaataggt aatattgcat ttgaatgtaa attagttaat gcagtaaata atgattgagt
      781 atacacatct tcatagaata aaccatagta tgcatcatcc acaacagcta taacttttgt
      841 acctttatta gctaatgctt taattgcttc gacgatcgta gtcacttctt tatgcgtagg
      901 tgtgtaacct gtcggattat taggataatt taaaatcata atgactttat ctttattgta
      961 tgattgtaaa gcttctacta gtgaatcagt agtataatgc ccgtctttat caaagatagg
     1021 atatgtttga aggtttgcac catttcttgt attgaaaaca agtttgtaat taccccaatt
     1081 atgctctggt aacaagatag tgtcatcttg atttacaaat aaatcgccaa ctagagataa
     1141 accatgtgtt aatgcattcg taacaattgg tcgtgacatg ttgtcgattg atagctctgg
     1201 attgtcacgc aacatttttt gttgccataa atcacgtaat tcttcgatgc cttgtggtgg
     1261 cgcataaggg aatatttcat ctggagttaa atcattaaac attgcatcta aagatgatgc
     1321 aaacattttt ccgtctttat ttgtcgccat acctatagtt gcattatatg ttgtactctt
     1381 tgcttcagca gattgagata aaatgccctt tggataaaac atattttgac ctaagtcaga
     1441 taacattgca aaggcagttg catttgactg ttgaagttgt tcatttaagc tttgggctaa
     1501 aggattcata attgcttaac ctctctttaa aaaaattgta tgcctatata ttatcttcaa
     1561 ttataccaaa gataaagtgt tgttggtgaa ttaacgaata atatgtaaaa tccgctacca
     1621 aaatacgaac acatgttctg tttttgtgtt agaataggtg taggtcatcc ttcagtatga
     1681 tactttagtc gaacggaggt gtagtgtaat gatgtggtgg caagaaggga tgatgacact
     1741 tattacaggt ggcttactaa ttatttttcg gctttggtta gaactgaaat ggaaaaataa
     1801 aaaatgatcc tacacacata aataacccca agtctgctgg taccagactt ggggttgtgt
     1861 agtataattc tgtggataca cttaaataat atcattgaac acaatatatc gcaatcaata
     1921 atcattttga atgcatagtt ttgttgtcat gtaagccaat gagagattgg ttatattaca
     1981 ctatacaata tattaagtta tgataaatct ttatatttgt tataatgtcg atgttgaggt
     2041 gaaataaatt gaaacatcta acaaaaatat ttgtagcatt agcaattatt ttatttattg
     2101 ttggttatta tttacaagca acaaaccatg agagccaagg tataaaacta ttattggcag
     2161 caattatgtt tatgatttgt gcttttataa gtagaagtaa tgatcgacgt aaaaataaca
     2221 aatagaattg actgttttaa acgactgttc ttaatgacgc gacaatcacc gtcaaagtat
     2281 aagaatattc gttttgacag tcttttttat tgaagtagac aatgcaagat taaatggtag
     2341 agtcatttca ttttattcaa ctgctagtaa tacaataccc agtgttattt ctgaagactg
     2401 gttgataatg aaacatgcat atttacaaaa ctgtgtatat tgtgtatatt gtatatatac
     2461 agaaggtgat agggggacgt tgatgaaaat aattttaaaa aacaatagtg attttccgat
     2521 ttatgaacag attaagcaac aagtaaaaca aaatatttta aagggacatg ttgctcctgg
     2581 agagcatttg ccatcaatga gggaacttgc caaagatctt caagtaagtt taattactac
     2641 caaacgtgct tatgaagatt tagagaaaga tgggtttgtt accacaatta gaggaaaagg
     2701 gacctttgtt aaggagcaag atagttctat tttaaaagag aaacaatttt ttaccattga
     2761 aaatttggtt aaagaattgg ttaatgaagc gcaagccatc gaaatgtcac ttgaggaact
     2821 tcaagatatt ttaacgttca tttatgagga ggaatcatca tgaatgccat agaagtaagt
     2881 aatgttaatt attcaagtga tcagtttaat ttaaagaata tctctttcaa agttcctcaa
     2941 ggattcgtca ctggttttat tggtagaaat ggcgctggta aaaccacaat aataaggtta
     3001 attatggatt tgtatcaacc acaaactggt gttattagag tattagaaga ggatatggct
     3061 ctcaatccga tagagttaaa gaatagaatt gggtttgttt acgctgaaaa ttattttaat
     3121 gagagatgga ctacgaagca acttgaaaaa atgattgccc ctttttatcg taaatgggac
     3181 catcaagtat ttgaatttta tcttgaaaag ttcgatttac caatcaataa atcaataaaa
     3241 acattttcga ctggcatgaa aatgaaatta tcattagcag ttgcattttc acatcatgct
     3301 gaattgtata tttttgatga gcctacttca ggtcttgatc ccttagctag aaatgaacta
     3361 ttggagataa ttcagcaaga attaattgat gaaaataaaa caatattcat gtcgacacac
     3421 attatctcag atttagaaaa aatagctgat tatatcattc acttaagtga cggtgaagtt
     3481 atcttaaatg gttcaaaaga acaactgtta caaagatatc aagttgtgag tggtgcaatt
     3541 gaggaccttg atgatgaatt agcatctcta cttatttttg aagaacataa gcgtacagga
     3601 tttataggat taacagaaca tgcgcaagta tttaaagaaa ttttaggtca taaagtgaat
     3661 atcactacac cttctattga aaacttaatg gtttatcttg aaaagagaaa acctaaatat
     3721 catgaaaaca tcaaattgat ggaagaaggt ttttaaatga aggcattgtt aattaggaat
     3781 tttaaattac gacgatatac acttatcatc tatgttctat tattaacctt atatcctttt
     3841 tatataatgc ttgactcaac aaagttcttt tacctattgc aatcttttat aagtccaaca
     3901 atattgatta tttggatttt agatgctggt cacttattta gactcaatcg tagattaggt
     3961 ggtaatgatt catattattt ttatatgagt ttacctgtgt caaaaaaaca attgcttaat
     4021 gcgaactata ttacatgcat tgttttaaca ttagttggaa cgcttgttat tagtttatat
     4081 gcttatgaag cagatgtgat tgaaccaaat tcaatttatt tttcaactgc atatgcattt
     4141 gtcatatcta acttcttgtc tataccaatt gcatttagtc aatttacaga attgcgcaga
     4201 gtcaaagtgc cctatggtat atacgtgttt actattatca ttttagttcc atttttattt
     4261 tcaattgcaa tagtgttggt gaattatttt gttctaagtc aatcatcatt cccagatcta
     4321 tattcatata ttttaaatat tggttttcta attataagca ttgttatact tattgttaat
     4381 tattttaaac agcttaataa aataaatact agaaaattta aaggaggcag tcgatgaaat
     4441 tagaacatat tacaaaaaaa tacggctcaa atgtcgtttt aaatgatatt gattttgact
     4501 ttggcgatag tagaattgtt ggattaatag ggaaaaacgg tgttggtaaa acaaccgtta
     4561 tgaaagtaat gaatggtaat attattaaat ttgatggaaa agtagatatt gataatgcag
     4621 ataatatcgg ttttttaatt gagcatccta aattatatga taataaatca ggattgtata
     4681 acttgaaatt atttgcacaa gtattaggta agggttttga taaagcatac acagacaaaa
     4741 ttatagatgc atttggtatg agaccttata ttaaaaagaa agttaagaaa tattcaatgg
     4801 ggatgaagca aaagttagca attgcagtat ctttaatgaa taaacctaaa tttttaatct
     4861 tggatgagcc tacaaatggt atggatccag atggctcaat tgatgtgctg actacaatta
     4921 agtctctagt aaatgaactt gatatgagaa ttctaatatc aagtcataag ttagaagata
     4981 ttgaattaat ttgtgataga gctgtatttt tgagagacgg acattttgtt caagatgtaa
     5041 acatggagga aggtgttgca tctgacacaa cgatagttac tgttgatcat aaagactttg
     5101 atagaactga aaaatatctt gcagagcatt tccaattaca aaatgtcgac aaagcagatg
     5161 gacatttaat gatcaatgca caaaaaaatt atcaagttat actaaaagca ttatctgaat
     5221 tagatattta tccgaaatat attgaaacac gtaaaagttc attgcgtgat acgtacttca
     5281 atataaatca aagaggtgat aaataatgag aattttaaat ttagttaagt atgattttta
     5341 tagtatattt aaaagtcctt taacatattt agcgatacta gtcgtatcta gtttgattgc
     5401 aacgcaaagt atacttatgg caaattcgat ggataacccg aaacatatta ttgtctatgg
     5461 atctgtattt gctgcagcaa aatggttatt gttaataatt ggattaatgt ttgttgttaa
     5521 gacaattacg cgtgattttt cacaaggtac aatccaacta tatatgagta aagttaaaac
     5581 acgtgttgga tacattattt cgaaaacaat ttcaattatt ttaatttcaa tattatttgc
     5641 attaattcat tatgtgattt tgattgttgt gcaggcatct agtaatggga aaaatttggc
     5701 gttttctaaa tatgtagata atttatggtt cttcctaatc tttttactat tctttggctt
     5761 gtttttattc ttaatcacac ttgcatcaca aaaaacagca atgatatttt cattaggtgt
     5821 atttttagta cttattgtac cgtttattaa accattcatt acatttattc caagatatgg
     5881 tgataaagtt ttagatgctt ttgattatat cccttttgct tacttaactg ataaaatgat
     5941 tagctctaac tttgatttta gcaattggca atgggtaatt tcattaggtt ctatagtgat
     6001 attcttcatt ttgaatatct tatatgtcgc taaaaaagac atttaataaa aaattttgag
     6061 gttgggaatt ttaaattttc ccaacctcaa agtttgtctt attgtaaatt tatttatttt
     6121 ctaatttgtt taggatggaa ttataaactg ctttccaaaa tgaagcgtca gttttatagc
     6181 gatttgatat aactaagtgt gtttcttttt ctaaatctgc atagtctgga tgatctttgc
     6241 tcggtaattt atcggcacga acatcagtta caaatttttg gacttcattt gctcttggtc
     6301 cccaaactgt ttcttgttcg aattgatcat tcaagaatac gaagatagga attgcacgtg
     6361 atttaccatt tgttaaatat tgatcgatca gttttgtatc atcatctcta tggaacacgc
     6421 gcacttctaa atttaatgct tcactgatgt gttttagaat tgggagattc atcattgcat
     6481 ctccacacca gtcttcagta attactaata ctttagaata attcatctct tttatttttt
     6541 tgatgcgtga atcatcttct ggtaactcaa atgattgata gatactgaga acggtatctt
     6601 gatttgtctt cattccatca atgtattcat ttaagggttg gctatttttg aaataagttt
     6661 ctaaatttgt cattgtaaaa acctccttta gcatttacaa cattatacca atttatagta
     6721 ataaaaggta atgaaataaa ttaattgcaa attctttgtt aatttttgtt aaggatgaaa
     6781 cgggaagcac cttatgctat atttaaataa gtacaaagaa agggtgacat cagtgcgtat
     6841 tcaaaatcgc tgggttgtgt ttatattatt tttaatctgt tcttttggtg tattaattgg
     6901 tttatatcaa tatcgtcata cgaaaactgt ggatttgtct aatcttgaaa taaacgatat
     6961 taaattaaat gagaagtttg ataaaaaagg ctatgaagta aataaaaaaa ttaagtttga
     7021 tcgttttaaa ttttataaca gtaaagctca ccctgacctt accgttaaag tgagagaaaa
     7081 ggataacatc gttaagggga taatattagt aagagatgaa aagatacata ctaattttga
     7141 tggaggaatt ggttcgccga taaataacgc gattgaaaat cttggattcg gatataaaag
     7201 aacaaaagtt ggcaatgatt tctcatcggt aaagtatatt gatagagata accatttaaa
     7261 attaaactta ctttatcaag atttagaaat taaacgtatt gaattttt
//
LOCUS       DATTQF010000118         7291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.118, whole genome shotgun sequence.
ACCESSION   DATTQF010000118 DATTQF010000000
VERSION     DATTQF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.118"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(43..318)
                     /locus_tag="VB241_002112"
     CDS             complement(43..318)
                     /locus_tag="VB241_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP1245388.1"
                     /translation="MEYEYPIDLDWSNEEMILVINFFNHVEKYYESGVTAGDFMDAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            complement(489..1895)
                     /gene="lpdA"
                     /locus_tag="VB241_002113"
     CDS             complement(489..1895)
                     /gene="lpdA"
                     /locus_tag="VB241_002113"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP1245389.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            complement(1899..3191)
                     /locus_tag="VB241_002114"
     CDS             complement(1899..3191)
                     /locus_tag="VB241_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEP1245390.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            complement(3282..4259)
                     /locus_tag="VB241_002115"
     CDS             complement(3282..4259)
                     /locus_tag="VB241_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP1245391.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            complement(4263..5375)
                     /gene="pdhA"
                     /locus_tag="VB241_002116"
     CDS             complement(4263..5375)
                     /gene="pdhA"
                     /locus_tag="VB241_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEP1245392.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQTAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            complement(5546..6172)
                     /locus_tag="VB241_002117"
     CDS             complement(5546..6172)
                     /locus_tag="VB241_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HEP1245393.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            6537..7088
                     /locus_tag="VB241_002118"
     CDS             6537..7088
                     /locus_tag="VB241_002118"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP1245394.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT         2131 a         1447 c         1092 g         2621 t
ORIGIN      
        1 ttaaagaaaa aatcctatta aattgtgtta atttcgaacg aattattttt tagctgttac
       61 tctttgttct tcagagtgag tttttacatc ttgaactgct ttgtaactat tatagccact
      121 acttttttcg aaagtattaa aaatttgttt ttcctctgct ttagcaggca caatttcttt
      181 aaatctttta tatgcatcca taaagtctcc tgccgtcacg ccggattcat aatacttctc
      241 tacatgatta aagaaattta tcactaaaat catctcttca ttactccagt ctaaatcaat
      301 tggatactca tattccattg ttgaaccctc catttcaatt cttttgttag catcgctagt
      361 ataacatgca tgtcacatgt taacgatatc tcaaatcaat ttccttacac tactttatta
      421 cctaaaacaa aaaagccgta gcatatgcta cagcttttaa aaactgaatc tttatagata
      481 atcagttatt acattgtatg gattgggtat ccgatagctt tttcagctgc ttccatagtc
      541 atctcaccta atgttggatg tgcatggatt gttaatgcga tatcttcagc attcatacca
      601 gcttcaattg ctaaacctaa ttcagagata atatctgatg caccagtacc aactacttga
      661 gcaccgatta aagtatcatc ttctttaagt gtaataagtt taacaaatcc gttagtatcg
      721 tctaatgata atgcacgacc atttgctgca tatggaaatt tagaagcttt aattgctaaa
      781 ccttcttctt tagcttgcgc ttctgaataa ccaactgtag ctaattcagg ttcagtaaag
      841 catactgctg gcataccaat gtaatcaact tcagcagctt gaccatcaat tgcttcagca
      901 gcaactttag cttcatagct agctttgtga gcaagtggta aacctggaac gatatcacca
      961 attgcataga tattgctgat agacgtacgg ctttgtttat caacttctaa taatccacgg
     1021 tcagcgaatt taacacctaa ttcttctagg cctaattcgt ctgtgtttgg acgacgacct
     1081 acagttacta atacataatc agcttcgatt gttttctctt cgcctttagc ttcataagta
     1141 actttaactc cgttatctgt ttcttcagct gatttagcca ttgcttcagt aacgatttca
     1201 acaccttttt ctttcatacc ttttttaaca ggttgtgtca tttgtttttc gaagccacct
     1261 aagatatctt tagcaccttc aaggatggtt acttctgaac caaagttagc aaatgctgta
     1321 cctaattctg atccaatgta tcctccacca actacaacta atttacctgg tacttcttgt
     1381 aaatttaaag cacctgttga gtcgataaca cgtttaccga atttgaaatt aggaatttca
     1441 attggtcttg aacctgttgc aatgattgcg tttttaaagt tgtatgtttg tgcgctcttt
     1501 tcgtccataa cacgtaagct attgttatct acgaaatatg cttcaccttt aacgatgttt
     1561 actttgttac ctttaagtaa gccttcaaca ccaccagtta atttattaac aactgatgat
     1621 ttgaattctt gaactttttg gaagtttaaa gaaacacttt cagcaataac acctaagttt
     1681 tcagaatgtt gtgcttcaac aaaacggtga gaagcatgta gtaatgcttt tgaaggaata
     1741 catcctacgt ttaagcaaac accaccaaga ttacctttct caacgattgt tactttttgt
     1801 cctaattgag ctgcacgaat tgctgcaacg tatccaccag gacctgctcc gattactata
     1861 gtatctgttt caattgggaa atctccaact accatgtttt acccctccat taataataat
     1921 tctggattat ttaataagcg tttaatgtga ttcattgcat tttggccagt tgcaccatca
     1981 atttgtctgt ggtcaaagct taatgataat gctaatactg gtgctgcaac aatttctcca
     2041 tctttaacga taggtttttg agcaatacgg ccaattccta agattgctac ttctgggtga
     2101 ttaataactg gagtgaacca ttgtccacca gctgaaccga tattactgat tgtgcatgtt
     2161 gcacctttca tttcatcggc tgttaattta ccatcacgtg ctttaacagc taattcgtta
     2221 atttcatcag aaatttggaa aatagattta cggtcagcat gtttcactac aggtactaat
     2281 aagcctctat cagtgtccgc cgcaataccg atattccagt aatgtttatg cacaatttca
     2341 ccagcttctt cattgaatga agtattaagt gctgggtatt ttttcaatgc agaaacaagt
     2401 gctttaacaa catatggtaa gaatgttaac ttagtacctt gttcagctgc gatttcttta
     2461 aatttcttac ggtgatccca taatgcttga acatcaattt catccattaa tgttacatga
     2521 ggtgcagtat gcttagagtt aaccattgct ttcgcaattg ctctacgcat agcagggatt
     2581 ttttcagttg tttctgggaa gtcgccttct aatgatactg ctgcaggtgc tgcaggagtt
     2641 tcagcaactt cttcatttgt agctgaagca gctgattcat ttgaagctgt tggtgcacca
     2701 ccatttaagt atgcatctac atcttctttt gtaatacgac catttttacc agatccagaa
     2761 actgctttaa tgttaacacc tttttcacgt gcgtatttac gtactgaagg cattgctttt
     2821 acagttctgt tttcatctac ttcttcagtt tgagtagcta caggtgcttg ctctgctggc
     2881 gcttcctctt tcgcaggttc ttctttagat gatgaatcat catcatgacc tttaaattgc
     2941 atatcttctg catcaggtgc atcgatttta acaataacgt caccaactac agctactgta
     3001 ccttcttcta ccataacttc ttctacagta ccagatactg gtgatgggat ttctactact
     3061 gatttatcgt tttgtacctc agctaaaaca tcgtcttctt caatagtatc tccagcttta
     3121 acaaaccatt ttacaatttc accttcgtgg ataccttccc cgatatcggg taatctaaat
     3181 tcaaatgcca cgtttttgcc ctcctaagat ttcgtaaatt aaacatttca ttttaatcaa
     3241 tgagactaaa atacgctaac ttcgttaact tttacaatgt attaaaattc taaagtttct
     3301 tttgcttttt cgatgatgtc atttttgttt ggtaaccaaa cattttcagc ttgagtgaat
     3361 ggataaattg tatctgctgc tgcaactctt ccaataggtg cttctaatga aaggattgca
     3421 cgttcactta attcagctac aactgctgca ccaacaccag cttgacgttg tgcttcttga
     3481 actacaactg cacgaccagt tttttcaact gaagctacaa ttgtatcaac atcgattggt
     3541 tgaacagtac gtaagtcaat tacttcaaca gaataaccat ctttttcaag ttcttctgca
     3601 gctttcattg attcttgaac cattgcaccg tatgtgatga ttgaaatgtc attaccttct
     3661 tttttcacat tagccttacc aatgtcaatt gtatattctt cttcaggtac ttcttcacgg
     3721 aatgaacgat acaatttcat atgctctaag tatacgactg ggtcattact tctaatagaa
     3781 gaaattaata aacctttcgc atcgtatggg cctgaaggaa taacaacctt tagacctgga
     3841 gattgagcta aaataccttc taagttatct gcgtgcaatt caggtgtgtg tacgccacca
     3901 ccaaatgggc tacgaattgt tacaggtgca gttttagtac cgcctgaacg gaaacgagta
     3961 cgtgcaattt gtccagcaat cgcatcaaat acttcgaata cgaaacctaa gaattgtact
     4021 tccataaccg gacggaatcc ttcaactgca agacccattg ctaaaccacc aatacctgat
     4081 tcagctaaag gtgtatcgaa tactctatct tcaccaaatt ctttttgtag tccttcagta
     4141 acacggaaaa caccgccgtt aacaccaacg tcttcaccaa aaattaaaac atcttggtca
     4201 tttttaagtt cagttttaag cgcatcatta atcgcttgaa ccattgtcat ttgtgccatg
     4261 gcttacttcg actccttctc tttgtatatt tcatattgtt ctgctaagtt ttgaggcata
     4321 tcttcataca taatttccat tagagaagta acagtttgtt tttcagtgtt atcagcctct
     4381 ttaattgctg ctttaatatc agcttttgca cgttcaataa cttcattttc tttgtcttca
     4441 ttccataaac ctttgttttc aaggaattta cggaaacgta ctaatgggtc ttttttctcc
     4501 cattcagcat cttcatctga agttctgtaa cgagttggat cgtcaccagc cattgtatga
     4561 ggaccataac gatatgtcat agtttcaatt aatgttgggc cttcacctgc aactgcgcgg
     4621 tcacgtgctt ctttagttgc ttgatataca gctaacgcat ccataccatc aacttggata
     4681 ccaggaatac ctacagcaat tgctttttga gctaatgttt cagcagcagt ttgcttgctt
     4741 cttggtgttg aaattgcata gttattgttt tgaataacga aaattgcagg tgctttataa
     4801 gctgctgcaa agttaatacc ttcgtagaaa tcaccttgtg aagaaccacc gtcaccagtg
     4861 taagtgattg caactgcatt tttaccacgt tttttaagtg caaatgcaac gccagcagtt
     4921 tgaatgtatt gtgcaccgat aataatttgt gggcttaatg cattaacgcc ttcagggaat
     4981 tgatttcctt tgaagtgacc tcttgagaat aagaaagctt cagttaatgg taaaccatgc
     5041 caaataattt gaggaacatc tctgtatcct ggtaaaatgt aatcttcttt ttctaaagcg
     5101 tattgtgacg ctaattgtga tgcttcttga ccagcagttg gtgcatagaa acctaaacgt
     5161 ccttgtctgt ttaatgagat agaacgttga tcaaggatac gagtccatac cattctttcc
     5221 attaattcta ctaattgttc atccgtaaga tcaggtacta agtcttcatt tacgacgtta
     5281 ccattctcat ccaaaatttg aaccatttca aatttcgatt gagtatcatt taaaactttt
     5341 actgcatcga attgggcttg taacttagga gccattcaat tcaccatacc tttcccataa
     5401 ttgtatagaa attcatttta tccttactaa ttgtaccaca aataaatggg actgttaaac
     5461 catttgaaaa agttctgttt taataaaaaa agatacagat aacaggaact gtattagttg
     5521 tttcctacca tctgtatcat attaattatt taaattgatc aacgtcttgc ttttctttac
     5581 caaccttatt tagtactttc gtatacttat ctgatacttc ttttaacttt ttatagttct
     5641 gttctattgc ttttgatttt tcattaacac cttgttgtgt cgcatcattt tgatttaaat
     5701 atttaaataa tgttttttct gagtttacag cctttttata tgctttcgcg taatcactgt
     5761 gcaacttata tttttctttt aatacatcat ctaattgttt tacttcttta cgtttaacat
     5821 cattatcaat attatcaacg tgactttttg ctttcttaaa gtcttgttca gacttcttaa
     5881 ttgcgtcttc ttctttttca aattccttta gacgatcatc ggcattttta attaagtctt
     5941 ttactgcttt accgcgaact gctgtatctt tactattgac atcttcagtt aattttttct
     6001 ttttctcatc taattcagca attttcttac caacagtttt aattggttct tcatcgtctt
     6061 taattttatc cacttgcttt aaatatgcct taatttcttt tttatccgta gtacatcctg
     6121 caagcaagac actagatgct aatactactg cgattgtttt tccaaatttc attacttgta
     6181 cctccacttg ctatttaaat taaatgataa ataaaatctg cttggaaatc aattttaaac
     6241 aattacatta cacatcttca ttactaagta tatctgttga cagttacgtc atttataata
     6301 taatttttaa agtcaagacg ataaataaat ttctaaagta tcattgcctg aaatcaacac
     6361 tttaatcatt ttttaggcaa ttagcatatc tgaaaattta catatactat ctaaaataaa
     6421 ccttggcagt agttggttaa ctttgtacat acttacacat aaaaatgtat gagtatctta
     6481 acaacctttt tactgtcatt caactaaaac aaattaaaat aaggaaggtg caatatatgt
     6541 taacaatgaa agacatcatt agagatggtc atccaacttt gcgtcaaaaa gcagctgagc
     6601 tagaattacc attaactaaa gaagaaaaag aaacattaat cgccatgaga gagtttttag
     6661 taaatagtca agatgaggaa atcgcgaaac gttacggttt acgttcaggc gttggtttgg
     6721 ctgcacctca aattaatatt tctaaacgta tgattgctgt tttaatacca gatgatggca
     6781 gtggcaaatc ttatgactat atgcttgtga accctaaaat tgtaagtcat agcgttcaag
     6841 aagcttattt accaactggt gaaggttgcc ttagtgtcga tgataatgtt gctggtctag
     6901 ttcaccgtca taatagaatt acaattaaag ccaaagacat cgaaggtaat gatatacagt
     6961 tacgactaaa aggatatcca gcaattgttt tccaacatga aattgatcat ttaaatggtg
     7021 taatgttcta tgatcacatt gacaaagacc acccattaca accacataca gatgctgtag
     7081 aagtttaaaa cacactatta ctaattatca aagcttagga taaatatgat gtcctaagct
     7141 ttttctttta caacttttcc taaaaaataa cagtaaaata ttttcacctt tctaccaaac
     7201 tttttatccc ctcatttaaa ttttaccgtt tcatataaaa tcctttaatt ctttcttaac
     7261 attaatttat aatctctaca tttattttaa t
//
LOCUS       DATTQF010000119         7285 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.119, whole genome shotgun sequence.
ACCESSION   DATTQF010000119 DATTQF010000000
VERSION     DATTQF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.119"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            151..942
                     /gene="pgeF"
                     /locus_tag="VB241_002119"
     CDS             151..942
                     /gene="pgeF"
                     /locus_tag="VB241_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEP1245395.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDCPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNNYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            966..1634
                     /locus_tag="VB241_002120"
     CDS             966..1634
                     /locus_tag="VB241_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEP1245396.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1631..2194
                     /locus_tag="VB241_002121"
     CDS             1631..2194
                     /locus_tag="VB241_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP1245397.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2206..2496
                     /locus_tag="VB241_002122"
     CDS             2206..2496
                     /locus_tag="VB241_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP1245398.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2606..3385
                     /locus_tag="VB241_002123"
     CDS             2606..3385
                     /locus_tag="VB241_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP1245399.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFDLTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKIESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3409..4005
                     /locus_tag="VB241_002124"
     CDS             3409..4005
                     /locus_tag="VB241_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP1245400.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVASNTSDNNQQSNDSETTKK"
     gene            4226..6979
                     /gene="ileS"
                     /locus_tag="VB241_002125"
     CDS             4226..6979
                     /gene="ileS"
                     /locus_tag="VB241_002125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP1245401.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            complement(7090..>7285)
                     /locus_tag="VB241_002126"
     CDS             complement(7090..>7285)
                     /locus_tag="VB241_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEP1245402.1"
                     /translation="AWQRGTNENTNGLLREFFPKKTDLAKVNQEQLNYALDSINYRPR
                     KCLNWKFPYEVLCDELLHLN"
BASE COUNT         2650 a         1033 c         1374 g         2228 t
ORIGIN      
        1 gtcattaaaa gtgtttaagt atcgtgtcaa aattaagtcg aaaattattt gcgcaacatt
       61 ttaactttaa acataaatgt tatattatat aattattaac tttgtacagt tagacgaaga
      121 taatttaaat gaaatgatgg tgacgatcga gtgaatgata attttaaaaa gcaaccgcat
      181 catttaatat atgaagaatt attacaacaa ggtattactc taggtattac aactagaggc
      241 gatggtttaa gtgactatcc taaaaatgct tttaatatgg cgagatatat tgatgattgc
      301 ccatataata ttactcaaca tcaattgcaa ttagctgaag aaattgcgtt tgatagaaaa
      361 aattgggtgt ttcccatcca aacacatgaa aataaagtcg cttgtattac caaggatgat
      421 ataggcacaa atatagacac tttaactgat gcgcttcatg gtattgatgc gatgtacaca
      481 tatgatagta atgtcttatt aacgatgtgt tatgcagact gtgtaccagt atatttttat
      541 agtacaaaac atcattttat tgcattggcc catgcagggt ggcgtggtac ctatactgaa
      601 attgtaaaag aagtgctaaa acatgtgaac tttgatttga aagacttaca tgtcgttatt
      661 ggaccatcta catcatcaag ttatgaaatt aatgatgata ttaaaaataa atttgaaaca
      721 ttgccaattg atagtgccaa ctatattgaa actagaggac gagatcgtca tggtattgat
      781 ttgaaaaaag ccaatgctgc attattaaat aattatggtg ttcctaaaga aaatatttat
      841 acgacagcgt atgctacatc tgaacattta gagttatttt tctcttatcg attagaaaaa
      901 ggtcaaacag gacgcatgtt agcattcatt ggtcaacagt aaacaaggag gagatatgtt
      961 tgcgtgtgaa agataattta caacaaatct caacacaaat taatgacaaa agtgaaaaaa
     1021 ataatttttc aacaaaacca aacgtgattg cagttacaaa atatgttaca atagagcgag
     1081 ctaaagaagc gtatgaggct ggaataagac attttggtga gaatagattg gaaggctttt
     1141 ttcaaaagaa agaagcatta ccatcagatg cggtgatcca ttttatagga tcattacaat
     1201 ctcgaaaagt taaggacgtt ataaacgacg tagattattt ccatgcttta gatcgattga
     1261 gcttagccaa agaaattaac aaacgtgcag aacataaaat taaatgtttc ttgcaagtga
     1321 acgtttcggg agaagcttct aagcatggta ttgctttaga agatgttgat cagtttatag
     1381 atgatcttaa aaaatatgac aaaatcgaaa ttgtaggttt aatgacgatg gcaccattga
     1441 cagatgatga agcatatatt agatcgttat ttaaacagtt acgtttgaaa aaagaagaaa
     1501 tacaacgact caatttagaa tatgcgcctt gtgatgaatt atcaatggga atgagtaatg
     1561 actatcttat tgcagttgaa gaaggtgcaa cgtttgttag aattgggact aaacttgtag
     1621 gagaagagga gtgagccact tggctttaaa agatttattt agtggatttt ttgtaataga
     1681 tgatgaagag gaagtagaag tacctgacaa acaacaacag gtaaatgaag cgccagcaaa
     1741 agagcagtca caacaaacaa caaaacaaaa cgcaatcaaa tcagtccctc aaaaatctgc
     1801 atcaagatat acaacaacgt cagaagaaag gaataaccgt atgtctaatt attcaaaaaa
     1861 taattcacgt aatgttgtaa ctatgaacaa tgctacacca aacaatgcat cacaagaaag
     1921 ttcaaaaatg tgtttattcg aaccacgtgt tttttcagat acacaagata ttgctgatga
     1981 gcttaaaaac cgccgtgcga cacttgtcaa tttacaacgt attgataaag tatcagcgaa
     2041 aagaattatt gattttttaa gcggtactgt ttatgcaatc ggtggagata tccaacgtgt
     2101 aggtactgat attttcttat gtacgcctga taatgtggaa gtagctggaa gcattacaga
     2161 ccatattgaa aatatggaac attcattcga ctaaggagtt aacatatgga tataaatgtg
     2221 ctagctacaa tttttaaatt tatccttttt gttgttgaaa tttattattt cggcatgatt
     2281 atatatttct ttacatcttg ggtaccaagt attagagaaa ctaaggtagg ttatttttta
     2341 gcgaaaatat atgaaccttt cttacaacca tttagaaaag taattccacc tattggaatt
     2401 atcgacatat catcaatcgc tgcaattatc gttttagtat tattccaaaa agggttactc
     2461 caaatcttta attggatttt aattcaatta caataaatta agcgacataa cgaggcaagg
     2521 gtacatgata ctttagcctc gtttttgata tgtatttttc taaatataag ggcaatagat
     2581 ggtattttat aattttttta aggtagtgat taacatagat atttatcaac actttagaca
     2641 ggaagaatac gaattaattg atcagctaac ggataaatgt gatcaagcgg aacagcatta
     2701 tgcaccagta ttaacgcatt ttttagatcc aagagggcaa tatatattgg aagtgatttg
     2761 tggcagttat gaagatttaa acgtatcttt ttatggcggg cctaatgctg aaagaaaaag
     2821 agcaatcatt tcgccgaact attacgaacc taaagaaagt gactttgatt taactttaat
     2881 ggaaatagat tatcctgaaa aattcgtcac tttaaaacat caacatattt tagggacatt
     2941 aatgtcttta ggtatcgaac gcgaacaagt tggagatata attgtgaatg aacgaattca
     3001 atttgttttg acaagtagat tggaatcatt tattatgtta gaattacaac gtattaaagg
     3061 cgcatcagtt aaactttata ctattccagt aacagatatg atacaatcta atgagaattg
     3121 gaaaattgaa agtgcaacag ttagttcttt aaggttagat gttgttatta aagaaatgat
     3181 acgtaaatca cgtacgattg cgaaacaact aatcgaaaaa aaacgtgtta aagtgaatca
     3241 cactattgtt gattcagcag attttcaatt acaagcaaat gatttaatat ccatccaagg
     3301 ttttggtaga gcacacatta ctgacttagg tggtaaaact aaaaaagata aaacgcacat
     3361 tacctataga acattattca aatagtaatg atttaaggag gataacaaat gccttttaca
     3421 ccaaatgaaa ttaagaataa agagttttca cgtgtgaaga atggtttaga acctactgaa
     3481 gttgctaatt ttttggagca actaagcact gaaattgaac gtcttaaaga agataaaaaa
     3541 caacttgaaa aagtaatcga agagagagat actaatatta agtcttatca agacgtgcat
     3601 caatctgtaa gtgatgcttt gatacaagct caaaaagctg gtgaagaaac taagcaagct
     3661 gcagagaaac aagctgaagc gattatagct aaggcagaag cacaagctaa tcaaatggtt
     3721 ggtgacgcgg tagaaaaagc acgccgttta gcattccaga ctgaagatat gaaacgtcaa
     3781 tcaaaagtat ttagatcgcg tttccgtatg ttagttgaag cgcaattaga cttattaaaa
     3841 aacgaagatt gggattactt gttgaattat gatttagacg ctgaacaagt gacgcttgaa
     3901 aatattcatc atttgcatga aaatgattta aagccagatg aagttgcatc aaatacatca
     3961 gacaataatc aacaatccaa tgattcagaa acaactaaga agtaagaatt aaataaagac
     4021 agacgcgtaa tatacattta acttttcaca gcgaattagg taatggtgag agcctagtaa
     4081 aagcatgtat gttatatcac tggcttttta atatttaaat aatgtaatga gagaactcta
     4141 agttgagtta ataagggtgg taccgcgagc aatcgtccct tttaatttaa cttagagttt
     4201 tttaaatttt taaggagtga aaaaaatgga ttacaaagaa acgttattaa tgcctaaaac
     4261 agatttccca atgcgaggtg gtttaccaaa caaggaaccg caaattcaag aaaaatggga
     4321 tgcagaagat caataccata aagcgttaga aaaaaataaa ggtaacgaaa cattcatttt
     4381 acatgatggc ccaccatacg cgaatggtaa cttacatatg ggacatgcct tgaacaaaat
     4441 tttaaaagac tttattgtac gttataaaac tatgcaaggg ttctatgcac catacgtacc
     4501 aggttgggat acacatggtt tgccaattga acaagcatta acgaaaaaag gtgttgaccg
     4561 taagaaaatg tcaacagctg aattccgtga gaaatgtaaa gaatttgctt tagaacaaat
     4621 tgaattacag aaaaaagatt ttagacgttt aggtgttcgt ggtgacttta atgatccata
     4681 tattacatta aaacctgaat acgaagctgc acaaattcgt atttttggag aaatggcaga
     4741 taaaggttta atttataaag gtaaaaagcc agtttattgg tctccttcaa gtgagtcttc
     4801 attagcagaa gcagaaattg aatatcacga taaacgttca gcatcaattt acgttgcatt
     4861 taacgttaaa gatgacaaag gtgtcgttga tgcagatgct aaatttatta tctggacaac
     4921 aacgccatgg acaattccat caaatgttgc gattaccgtt catccagaat taaaatacgg
     4981 tcaatacaat gtaaatggcg aaaaatatat tattgcagaa gccttatctg acgccgtagc
     5041 agaagcactg gattgggata aagcatcaat caaattagaa aaagaataca caggtaagga
     5101 attggagtat gttgtagcac aacatccatt cttagataga gaatcgttag tgattaatgg
     5161 tgatcatgtt actacagatg ctggtacagg ttgtgtacat acagcaccag gtcacgggga
     5221 agatgactat attgttggtc aaaaatatga attgccagta attagtccaa tcgatgataa
     5281 aggtgtattt actgaagaag gcggccaatt tgaaggaatg ttctatgata aagctaataa
     5341 agccgttact gatttattaa cagaaaaagg tgcactatta aaattagact ttattacaca
     5401 tagctatcca cacgactgga gaacaaaaaa acctgtaatt ttccgtgcta caccacaatg
     5461 gtttgcttca atcagtaaag taagacaaga tattttagat gcaatcgaaa atacaaactt
     5521 caaagtaaat tggggtaaaa cacgtattta caatatggtt cgtgaccgtg gcgaatgggt
     5581 tatttctcgt caacgtgttt ggggtgtacc gttaccagta ttttatgctg aaaatggcga
     5641 aattatcatg acgaaagaaa cagtgaatca tgttgctgat ttatttgcag aacacggttc
     5701 aaatatttgg tttgaaagag aagcgaaaga cttactacca gaaggattta cacatccagg
     5761 cagccctaac ggtacattta ctaaagaaac agacattatg gacgtttggt ttgattctgg
     5821 ttcatcacac cgtggcgtgt tggaaacaag accggaatta agtttcccag cagatatgta
     5881 tttagaaggt agtgaccaat atcgtggttg gttcaactct tctattacaa cttcagttgc
     5941 tacaagagga gtatcacctt ataaattctt actttctcat ggttttgtta tggacggtga
     6001 aggtaagaaa atgagtaaat ctttaggtaa tgtgattgta cctgaccaag tggttaaaca
     6061 aaaaggtgct gatattgcga gactttgggt aagtagtacg gactatttag ctgatgttag
     6121 aatttctgat gaaattttaa aacaaacatc tgatgtttat cgtaaaatca gaaatacatt
     6181 aagatttatg ttaggtaata ttaatgattt caatcctgat acagatagca ttcctgaatc
     6241 agagttatta gaagttgatc gttacttgct aaatcgttta cgtgaattta ctgcaagtac
     6301 gattaacaac tatgaaaact ttgactactt aaatatttat caagaagttc aaaactttat
     6361 caatgttgag ttaagtaatt tctatttgga ttacggtaaa gatattttat atattgaaca
     6421 acgtgattct catatccgtc gtagtatgca aacagtgtta tatcaaattt tagttgatat
     6481 gacgaagttg ttagcaccaa tcttagtgca tacagctgaa gaagtttggt ctcatacacc
     6541 acatgttaaa gaagaaagtg ttcacttagc agacatgcct aaagttgtag aagtagatca
     6601 agctttattg gataaatggc gtacatttat gaatttacgt gatgatgtga accgtgcatt
     6661 agaaactgct cgtaatgaaa aagttattgg taaatcatta gaagctaaag ttacgattgc
     6721 tagtaacgat aaatttaatg catctgaatt cttaacttca tttgatgcat tacatcaatt
     6781 atttatcgtg tcacaagtta aagttgtaga taagttagat gatcaggcaa cagcttatga
     6841 acatggtgat attgtcatcg aacatgcaga tggtgaaaaa tgtgaaagat gttggaacta
     6901 ttcagaggat cttggtgctg ttgatgaatt gacgcatcta tgcccacgat gccaacaagt
     6961 tgtaaaatca cttgtataat tgaaattgta taaagtactt atacagatga tataaattaa
     7021 agctctcttc ataatcatgt tgtagttttt gttgacatga tgaagagagt ttttttgaga
     7081 gttgatttgt caatttaagt gcaacagttc atcacataaa acttcataag gaaacttcca
     7141 atttaaacac tttcttggtc gataattaat tgagtctaat gcatagttaa gttgctcttg
     7201 atttacttta gctaagtctg ttttcttagg gaaaaattct cttagtaaac cattggtatt
     7261 ttcatttgta cctctttgcc atgcg
//
LOCUS       DATTQF010000120         7247 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.120, whole genome shotgun sequence.
ACCESSION   DATTQF010000120 DATTQF010000000
VERSION     DATTQF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.120"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            45..149
                     /locus_tag="VB241_002127"
     CDS             45..149
                     /locus_tag="VB241_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245403.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            124..438
                     /locus_tag="VB241_002128"
     CDS             124..438
                     /locus_tag="VB241_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245404.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(730..1533)
                     /locus_tag="VB241_002129"
     CDS             complement(730..1533)
                     /locus_tag="VB241_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1245405.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQDDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEITLDHNDNGIPHALKKLL"
     gene            complement(1591..2505)
                     /locus_tag="VB241_002130"
     CDS             complement(1591..2505)
                     /locus_tag="VB241_002130"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEP1245406.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVAAKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVTKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            complement(2507..3568)
                     /locus_tag="VB241_002131"
     CDS             complement(2507..3568)
                     /locus_tag="VB241_002131"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEP1245407.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSEILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPLR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(3574..4854)
                     /locus_tag="VB241_002132"
     CDS             complement(3574..4854)
                     /locus_tag="VB241_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEP1245408.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QAYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            5045..6427
                     /locus_tag="VB241_002133"
     CDS             5045..6427
                     /locus_tag="VB241_002133"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEP1245409.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(6481..7056)
                     /locus_tag="VB241_002134"
     CDS             complement(6481..7056)
                     /locus_tag="VB241_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245410.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDNTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
BASE COUNT         2359 a         1292 c         1025 g         2571 t
ORIGIN      
        1 ataaagtgta ttaactagta ttctgtttat attttattat gaccatgtta ccattcttta
       61 ttttagatat tttgcatatc gtgtataata tagataacaa taacttttca ggggtgaaat
      121 tcaatgattg gacaaacaaa tttatttgat gatgtactaa agcaagatga gagatttgtt
      181 attgtagttc aagcattaga agaaaagaac ggacaattat taaagagaac tttaagggaa
      241 tatcccggtt taaaccataa acaaatgaat gatttattta cgcacttaaa ggaattattt
      301 tccgaagaat catttgctga aaaccaatca gcgttcagta ttacagttta tacaaattta
      361 gattatactg ctgaccaaat atatgctcat gtaaaacgct tcagagggaa gcatgactgg
      421 acacaaacag ctaaataaaa tcaagatgct tgcagtgatt ttaacaatta atgttagatt
      481 gtcactgcat tttttaatac attcgttatt actcagaatg attcaaattt atttatctac
      541 cttccgtgac taattaatac ttgatttact taaaaaatat gcatctactc tattctttag
      601 atatcattac taatctataa tatgtatttc aaatgcgcca tattatagct aacgcatttt
      661 cacataaata aaaagccttg acacaagata tcttattcaa atatcctgtg cttaggctta
      721 ttttaaaatt tataacaatt ttttgagcgc atgtggaata ccattgtcat tatggtccaa
      781 cgtaatttca tctgcatatt gcttaagttc atctgatgca ttccccatag caatagctag
      841 acctgcgact tcaaacattg atttatcatt catactatca ccaaaagcaa tgacttgatc
      901 caccgaatat cccaatttgt gacataacgc tttaattgca ttaccttttg aaacatcttt
      961 agccataaat tctaagaaga atggcttact tgtcgtagca tctacattat cattgaacac
     1021 accattcaaa tcaattctag cttctgtaat attcgctaca taatctacac ccatgacctt
     1081 aggaacatca tcttgaatat acgctttaat gtcatcaaca cgtttcatcg gtaaaccagt
     1141 taattctgct tcaatattca tatattcatg ttcgctgtcg taaataattt gaccatcatg
     1201 atatgtaaga acgaaaaagc ctctatctcg acaataatct acaatttcat cgaaatcctg
     1261 cttaccaatc gatttactta cttctacttc ttcattagtc atgttaatcg ttttactgcc
     1321 gttataacta ataatatagc tatgatgttc aggtaatttt aaatctctag cagttggaat
     1381 cataccttca gtaggtctac cagatgctaa aacaacataa taaccttcat cttgaattgc
     1441 tgttaaataa tttgcagttt cttctgatat cacattatct gaatttagca atgtgtcatc
     1501 catgtctaaa acgacaactt tataatttgt catatcgcat taaatctcct ctctatttca
     1561 cattcaatac tattttacaa tataaagtgt ctattggtat acaattcgtt ctttaacacc
     1621 actaacatta atatccacta attcagaatg acatgtaact acatttctat ttaaagcgcg
     1681 aactaattcg ccactatttt ctttgcgact aaatattaaa atagttggtc cagcaccact
     1741 aattacagtt gcataggcat tatgttgact agcaattgtt ttcacttcat caaattcagc
     1801 aattaaatgc tgacgatacg gttcatgaaa gccatcttgt tgcatgagtt tacctgctaa
     1861 ttcataattg tgctgtgcta atgcacaaat cattgtatta ctaattgcac tacttttaac
     1921 ggcttcacta tgtgttaatt tttgtggtaa agcacgtctt gatgcttctg tttttagttc
     1981 ataagttggt atcgttacaa tcacatccac gtctggtatg tcgatatgtg caactgacgt
     2041 ttctttcgtg acatcattat aatatccagc gattaaacca ccataaatgg tcggcgcaac
     2101 attatcagga tgtccttcga tttcagtcgc taattgtaat acctcgtgtt tagatagttg
     2161 gatatcacca aaataatttg cgatatatat agctcctact aaagcagaag ctgacgaacc
     2221 taaccctctt gccaatggaa tatcacttct catttcgata cataaattag gcaagtcaac
     2281 actatattta gcagcaactt gttgtgccac atgataaata aagtttgttt cgtcagtagg
     2341 taattgcttc gatgcatcat catggaaaat atattcccat tttgtccctg atgtttcttt
     2401 tacagacaga tgcaaaaatt tatctaaagc catacctata gaatcaaagc caactccaag
     2461 gttggctgtt gatgcaggaa ttgttaactc caaaacattc gacatgttat aaagctcctt
     2521 taatataatc gataatgcta tctttatcat ttggcaatgg ctttatcgga ttatctaata
     2581 gtgaaatagc agtatctgga tcttttaacc cattaccagt taaaatcgca actacttttt
     2641 taccttgagg taatttacct tgtctgtgca atttaattaa acctgcaata gaagcattac
     2701 ttgctggttc actaaagaca ccttctttag ttgtcattaa ctgatatgct tctaaaattt
     2761 catcatcagt aacactatct attaatccat ttgattcttt aagagcatta gtcgctttat
     2821 cccaactagc aggattacca attcgaatag cagttgcaat cgtttcagga tttttaatga
     2881 ctttattttg aacaattggt gatgcacctt cagcttggaa gccaaacatt ttcggcaatt
     2941 gtgatccttt agcttcatga tattctttaa agcctttcca atatgcagta atattacctg
     3001 cattacctac aggaatcgct aaaatatcag gtgcttcacc gtctaattgt tgtacaattt
     3061 caaatgagcc tgtcttttgc ccttcgattc taagtggatt gacagagttt acaagctcga
     3121 tttcgccact ttttgcaatt tcttttacaa tttctaaagc ttcatcaaag tttccttcaa
     3181 tagaaacgat ttctgcacca tacattactg cttgcgacaa tttacctaat gcaattttac
     3241 cttctggtat tacgacgata gcttttaaac ctgctctcgc tgcatatgct gctgcagacg
     3301 ctgatgtatt tccagtcgaa gcgcatatta caattttctt accttgctct tttgcttttg
     3361 tcacagccat taccataccg cgatctttaa atgaacctgt cggattggca ccttcatact
     3421 tcacatataa atctatacct agtatttcag acatattttc acaatgaatg agtggtgtat
     3481 ttccctcatt caaagttaat tttggtgtat tttcatttac tggtaaatgt gctttaaact
     3541 cttctactaa tccttgccat cttctcatta taattaaact ccttctactg ggtatacttt
     3601 gtcaacttcg tatccatgct tagtgattaa ttcttccggt gatgattcca atccaactat
     3661 aacagcggca tatgcttgat tatcgtaatt tgcaactctt aatgatcggt gaaatggtaa
     3721 tattgccttt aactcatttt caaaattttc aattgaacct ttgacatgat tcactactac
     3781 aaagaaattg gatttttctt taatattaat ttctgcatca gaatccatca tttcccttgt
     3841 tttatctgtc tttaattcaa aatgtggtgg caatgtgtgt aaatctgatt caaagaataa
     3901 tgctacattc aataaatcac ttacaacggc actacctgtt gctaaactgc ctgctccttt
     3961 tccataaaac atcgtgtcac caacggcatc accaatgaca taaatcgcgt tatattcatc
     4021 ctctacagct gctaattgat gctttttatc aattaacgtt ggttctactg atgcattaac
     4081 tttgccattt tcatatattc ccttaccaat caatttaatt ttatacccca gttgatcggc
     4141 tacattaata tcagttaaag ttacaccact aattcctctt cgtttaacgt cgtttaattt
     4201 aatgacttgg ttaaatgata aatatgatgt aatgacaact ttacgcgctg catctacccc
     4261 ttctacatca tcagttggat ccgcttcagc aaaaccaagc cttttcgctt catcaagtgc
     4321 ttcctcaaat gtcgtttgct ctttagtcat ttttgataaa ataaaattag aggtaccatt
     4381 taaaattccc ataaattttg aaatattatt cgcattcaaa ccattattta tggcatttac
     4441 gatcggaata ccacctgcta cactcgcttc aaactttaaa gctacaccat tttcttctgc
     4501 taaatcttct aaaagtttaa gatgtactgc taataaatct ttatttgcgg taataacatg
     4561 ttttttattt ttaagtgctg ttcttaacca atctacagtt ggttcaattc ctcccatgac
     4621 ttcaacgata atatctaatg aatcatcatt taaaatttca ttaacatctt cagttaaatg
     4681 atattgacta atatttagcg gtctcttttt agatttatct cgaacaagaa tatgctttat
     4741 gacaatatct ttatttaatg tatcttgaat ttgctgtcgg ttctcttcga tgattttaac
     4801 aacaccagat ccgacagtac ctaatcctaa taatgctata tttaattttt tcattttatg
     4861 tacctccgtt tgtgcttttg agaaaaacaa atgtattatt gaaaaacact agatgatagt
     4921 atagtataga attattcttt atttttgtaa agttcgtaat attcaaaata taaatacata
     4981 atatcattct tacttacaaa actgaataca attttctgat tttttagaaa ggttggcatg
     5041 cactatgaaa gtttccaaat tcggtggtag ctccgtctcc aatgctactc aaattaaaaa
     5101 ggttttaaat attgttaatt ctgatccaga gcgaaagatt gttatcgttt ctgctccagg
     5161 taaaagacat gataatgata ttaaaacaac tgatttgtta atcagattat atgaaaaggt
     5221 cattaatcat cttgattatc atgataaaaa aagagaaatt attcagcgtt atgatgatat
     5281 tgtaaaagaa ttgcaaatgg atgaaagtat tttacggacg atagatgtga ctttggaaca
     5341 ttatattaat caattaaaaa atgaaccgaa gagactatta gatgcattac tttcttgtgg
     5401 tgaagatttt aatgcgcaat tgatagcttt atataataat agtcaaggta taccaacaaa
     5461 atatatatca ccgaaggaag cgggtatttt tgttactgac gtcccacaag aagcacaaat
     5521 attagatagt gcttactctg aaattaaaaa attaagagat tatgatgaaa aactaattat
     5581 cccaggattt tttggcgtat ctcatgaagg atatatagtt acatttccac gtggcggatc
     5641 agatataact ggcgccatca tatcaagtgg tgttagagct acaatttatg aaaatttcac
     5701 cgatgtgtct ggtatttata aagctaatcc aaatatcatc aaagatcctg aattaatcga
     5761 agaaatcact tatcgagaaa tgcgtgaatt atcttatgct ggttttagtg ttttccatga
     5821 tgaagcctta caaccacttt ataaagatcg catccctgta gttattaaaa atacgaaccg
     5881 ccctcaagat aaaggtacat ttatagtgca tgatagagaa ataaatgcga aaaatgtgat
     5941 tagcggtata agttgtgata aagattttac tgttattaat atcaaaaagt atttaatgaa
     6001 tagacaagtc ggctttacta gaaagatatt aggtgtctta gaggataata atatttcatt
     6061 tgatcatatg ccttcaggta ttgatactat cagtatcgtt atgcgttcaa aacaaattca
     6121 aaataaagaa acaaaagtat taaatgaaat tcgtcaaaaa tgtgatgttg atgagctaag
     6181 tattgagcat gatttagcaa ttttaatgat tgtcggcgaa ggtatgaata aagtcattgg
     6241 tactgcaaat aaaattacac atgccttagc tgaatcaaaa attaacttaa aaatgattaa
     6301 ccaaggtgct tcggaaattt cgatgatgtt tggaattcat gtagatgatg ctgaaaaagc
     6361 tgtattatct acatatgagt tttgctatca cggggttgca ttaaaaaact tgtgtgataa
     6421 aaactaatat aaaagcaatt cgtaacacat gtattcaact gcgttatgaa ttgctttttc
     6481 ttaattattt attgttctct ggattgcatc cttaatacgt tgctcaccta ctacaaattt
     6541 taaaataaaa aattgatagt agcttaatgc atctatgacg cgtttataat tggaataatg
     6601 atgatactga tggtgttgca ttagatcata cattagaaca atctctggtt taatataatc
     6661 cacatcccaa tttagactat ggaagtaaat gttactctta ggtatacgta tattcggatc
     6721 caatctaaaa atccattcat cgtttacaac atcataaacg aaaatactca ttattgttgt
     6781 attatcatat tgcacataaa cttttgcaat ttttgaaagt gataaatctt ctttagctat
     6841 tgcttgatct tgcttatcta tgtatagtaa cgcatatgat tgcggaataa ctttcacaat
     6901 atcacttaaa cgcttacgtt ccacgcatat ttcaacaata gaaggtaatt ttaattcatc
     6961 atttaagaaa agctgcatgg caacttcacc gtgaaattta aataattgcg atgatgtttt
     7021 cattaaattg tccactaatt gttctttagc attcatttca ttcactctca aatacacctc
     7081 ctaagcaagc gctttcacaa atatcattat acattttaaa attaatgaat tcaatcttca
     7141 aaatactatg agtgacctaa ttctgtaatt taaatgaccg ttataacata aattcgacat
     7201 aaacttgaat tcacgaatct ttcacgtcat attcattgaa catcaat
//
LOCUS       DATTQF010000121         7223 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.121, whole genome shotgun sequence.
ACCESSION   DATTQF010000121 DATTQF010000000
VERSION     DATTQF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.121"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            115..489
                     /locus_tag="VB241_002135"
     CDS             115..489
                     /locus_tag="VB241_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HEP1245411.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(739..1527)
                     /locus_tag="VB241_002136"
     CDS             complement(739..1527)
                     /locus_tag="VB241_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1245412.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVITYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFLSYYLNFLKSVS"
     gene            complement(1696..2412)
                     /locus_tag="VB241_002137"
     CDS             complement(1696..2412)
                     /locus_tag="VB241_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245413.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMVMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQTFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(2574..3080)
                     /locus_tag="VB241_002138"
     CDS             complement(2574..3080)
                     /locus_tag="VB241_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245414.1"
                     /translation="MKKLLTASIIACSVVMGVGIANTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(3216..4016)
                     /locus_tag="VB241_002139"
     CDS             complement(3216..4016)
                     /locus_tag="VB241_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP1245415.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSEDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            complement(4000..4719)
                     /locus_tag="VB241_002140"
     CDS             complement(4000..4719)
                     /locus_tag="VB241_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1245416.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKIDNKGENYGSIY"
     gene            complement(4774..5724)
                     /locus_tag="VB241_002141"
     CDS             complement(4774..5724)
                     /locus_tag="VB241_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP1245417.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQIIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(5874..6761)
                     /locus_tag="VB241_002142"
     CDS             complement(5874..6761)
                     /locus_tag="VB241_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1245418.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHEKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(7080..>7223)
                     /locus_tag="VB241_002143"
     CDS             complement(7080..>7223)
                     /locus_tag="VB241_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HEP1245419.1"
                     /translation="KKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNCDFLEPY
                     FKK"
BASE COUNT         2407 a         1248 c          925 g         2643 t
ORIGIN      
        1 acttatttaa tgcaaaatta tgactaacat atctataata aataaagatt agatatcaat
       61 atattatcgg gcaaatgtat cgagcaagat gcatcaaata gggaggtttt aaccatggca
      121 attacaaaaa tcaatgattg ctttgagttg ttatcaatgg tcacttatgc tgacaaatta
      181 aaaagtttaa ttaaaaagga attttcaatt agctttgaag aattcgctgt attgacatac
      241 atcagcgaaa acaaagagaa agaatactat cttaaagata ttattaatca tttaaactac
      301 aaacaaccac aagttgttaa agcagttaaa attttatctc aagaagatta cttcgataaa
      361 aaacgtaatg agcatgatga aagaactgta ttaattcttg ttaatgcaca acaacgtaaa
      421 aaaatcgaat cattattgag tcgagtaaat aaacgaatca ctgaagcaaa caacgaaatt
      481 gaactataat tttgtttagc gcaatttggt gaagtttgat agatgataca ttctattaaa
      541 cttccttttt ttatgctctt tttacctaat tgttaagcgg ttttgcacta atggcactta
      601 atataaggaa gatagccgct taacgagcag tatatatcaa atacatttca aaaaagctga
      661 tttctatcaa ataatgaata gtaatcagct ttttcacatt gcctaagggc tttatgccct
      721 agctttatat atcacatatt atgatacgct ttttaaaaaa tttagataat aacttagaaa
      781 tgcttctggt tcttcaatat gcggtgcatg tcctgaatgt tcgaagattt ttttcgttac
      841 atttaaaaaa tgcgcttcta atttaaaatg attcttatct ttaatcaaag gatcatgttc
      901 cccattaatt aacaaagtag gtacacatgt acgttttttt aaatacatag gttcatcttt
      961 atgcaaaatt aatgaacgaa ttgccaccgc actatcatcc tcagacttaa tgctatatat
     1021 tctttgtttt tgataccatt tcatcgtctt ctcttgattt ctaaacaatt tagaaaataa
     1081 aagaatatac gcttcagact tattaaaacc cgctatatca tcttgatatt gaattaacaa
     1141 ttgagtaaag ctgtgtatta agtcatcttt cttcgatgca atggtagtta atgattttac
     1201 acgataagca tatttatctg taaataactt agcaactacc ccacctaaat catgtcctag
     1261 aatatgtgcc tcatgaatat ttaacttctc cattaaaatt tttaaatcct caacgtgatc
     1321 gtttaaatcg tatgattcac ttttagaaga cttgccatga cctcttaaat cgtaggtaat
     1381 tactttatat gatgatgcta gttgatgttg caaatcttca aatccagcta aattaccatc
     1441 caatccatga agtaatatta taggatcccc tttaccttca atttgataag ttaattggat
     1501 ttgaggatta attgtgactt tattcattgt gacacctccc tcaataaact atatacccaa
     1561 ccttcattct atgtacgctt ccattttata aacaattttt ttaaattatt tataaattac
     1621 caaaagttat tccaacaata aaaaaactat tcatctcaat taatacaatt aatcaagtga
     1681 atagttttat catatttata atcttgtacg tgaagataat ctaattgtga aatatataac
     1741 tagaactact aataatatcc atataattaa tgaaaaagtc tgggctgatt gtggattatg
     1801 aatcattgca tctacggttt tttgacaaaa taataagccg attcccgaaa actcaagtcg
     1861 cttataaaat atgccggcaa ttactaaaat aataccaatg aaaaagaaaa attgatgtcc
     1921 aaaatttgta attggtataa cgccccaagt aatattaaat gcttgtaata taattaataa
     1981 cagggatatg atagcgataa ttgtacccat aaccatttct attttattac tacgcaagta
     2041 attcttttga gacaccctta ggtatacaac aatagtcatt ggaaaaatca ctaaacaata
     2101 aaatagactc attccagtaa tagcttgaaa ctgaacatac tgtttatcat ataaatagga
     2161 tatgagaatg aaatttataa taaagaaaat aactggattc atttgtaaag caaacaaatt
     2221 cctttgaatc attaatatga tttgtgctgg agattttctt ttatattcaa tataatcttg
     2281 atctttcttt tcagaatttt ttatatcagt aattactttc tctttaatat catcaatcaa
     2341 atgcggtgat aaacctttat gattaaagta atcttctata ttttctagta aaattttatc
     2401 gttaatacgc attggtagta ctcctttaaa atgaaacatc tttatttatt gtattggttt
     2461 aatatcccct tcgcaaatca taaaaattaa tattcactgt attatgccat gtaataaaat
     2521 aaccgacaag tattgcgatt taacttgtcg gttaagcacg attaatttaa ttattatttt
     2581 tccaaatcaa tacgatataa ttttatgttt ttatcttttg gtgctgaggc aaattgataa
     2641 gtcgttttgc ctttttcaat atatccaaaa ttaccagcaa ctttgctata ttccgtatgt
     2701 gttactgctt tgccaacata atttttaaca gcttgatatg ttggaccatt agcatcatta
     2761 tacatcattg aaatatgagt tactttacca ttgtttttct taccatcagt agttactaat
     2821 aacatacctt ttttagtatg gaattcgtaa taatgttcag ttccatcttc attgtaagaa
     2881 tacattggat ttttgtactt agttaaaaca tctttaatag attcgccaat tttcacattt
     2941 tctaatgact ggtcaccctt aattaattgt ttaacagtat caatagagtt gccacttgct
     3001 gcttcggcac tagtgttcgc tatgcctact cccattacaa cagaacatgc aattatactt
     3061 gccgttagta gctttttcat aagcactctc tccttttatt tatatcgtct tgtgctcaca
     3121 accattatac aacaatcatt tctaaaacaa cagtcatatt acaattatat tacaaataat
     3181 aattactact tttatatttc acttatcact aaaaattaaa catgcttttc atcgtgatgt
     3241 ttgttgataa atgaagctgt atcttcaacg aatctttctt gctcttcaac aaatgggaat
     3301 gcacttgatt cttgatatac ttcaaagtcc gcattttgga ttaaatcagc tacttcttta
     3361 gcttctaatc ttgttgttct ttcaccgaat tctcctgcaa taatttttgt cggtactacg
     3421 acatttctat aagtttgaga aatatcagcg tttttgaata cctctttaac tgcttgaatt
     3481 tcttctttag ttgatatttc atttgtatct acgacatgtt tgaggaatcg attcattttt
     3541 ctcggacgat aatacttacg cttatttaaa aatttatctt gtttttcagg atcccagttt
     3601 cgaataatat gggcatattt tctaaataaa cgttcttccg gtaattcacc ttcaatagat
     3661 gttggattta ccaacataag tgatgatgta aattcaggat aacgtactga aatatccgca
     3721 ccaatgattc cacccatttc atggcataca aatgcaactt cttcaatgta tagatattta
     3781 agtaattcaa caatatcttc agaaaaatct tttatttcaa tgtgacgagg tttatcagaa
     3841 tagccatgtc cacgtaaatc aattaacaca acttgaaatg attttgctaa ttctgctgct
     3901 aatttattaa acacagaata attatcaagt acagtatgaa tcaatacgat aggatagcct
     3961 tcacctaatg tactgtaatg tatcgatgtt ccatcttttc tagtaaatag atccataatt
     4021 ttctccttta ttatcaattt ttattggtaa aatggttaat aattcttcca ctgttgtaat
     4081 tgtaaaatca acttcttctt cgagtggttc aatttctgca tcttcttgtt tataccatac
     4141 actaaccatg cccatagctc gtgctggagc tacatcattc aatgcatcat caccgacata
     4201 cataatttgc tcaggtaata cccctagttg atcaatcata tcttcaaaaa ttttaggatg
     4261 tggtttacga aaaccaactg tttctgatgt tgataaataa ttaataacat gcatcaaacc
     4321 aagtgaatgt aatcgaaatt gtttaatctt agatttacca tttgcgataa caccaatttg
     4381 aaagcctttt tgcgatagct tttctagtgt atacaaagta tcgtaataag gaaatacata
     4441 acgataaaaa tgcatttcaa aatcgttaaa taagtctttc caagttaacc tatctacatg
     4501 gaaacgttta atgatttctt tatataaatc aggtttatca ttatcttcat cgtcatctag
     4561 ctcaataaat gcttttttaa aatctgccag ttgaacatga attaagtagt catgaaatcg
     4621 ttcatattgc tcttcgataa atttctctcg agatttaact ctgtccaaca acgtaccttc
     4681 taaatcaaat acaactgctt taatttgatt caaatccatc gcttatgccc tctttatgtt
     4741 tatagttatt tttattttag caaatgtcat gtgctagtgt aaccgtttcg actgagatat
     4801 tagcatgtaa aataacactg gtataccaat taatgcaatg ataatacttg ccggtatttg
     4861 tacgggtgac aatatatggc taccaataaa atctgccata accattattt gtcctccaat
     4921 tacaatattc aaagccaatt gtcttcctaa tgattgtttc cactgccatt ttctaatgag
     4981 ttgtggtatc accataccaa tgaatccaat tatgccaaca tatgcgacat ttatcgccgt
     5041 tatcatagaa gctatacata acgcgatata tgtaattatt tgaacattta agcctagtga
     5101 ctgacttttt agttctccta attgcaataa ctttagttga ttaagaatga taaatatacc
     5161 aaacagtgca atgataaatg tgattgctat tatggataca tttgagtatt ctgcatcacc
     5221 aaaaccacca aacagataat tggcaattgt atttaatttg cgaggtttta ataaaatcaa
     5281 aaaatataga agtgaattga atagtgcacc aatcattaaa ccacttaata ttaagattct
     5341 aactggatag ccttggctca ataccgacgt aatgactaat acagttatta atgttattaa
     5401 actaaatgtt attgaaaata caggaatcca taacgttgtt aatcctaaaa ataatgctaa
     5461 tcctgaacca aatgtagcac cgcttgccaa tcctaatgta aagctatctg ccaatgcatt
     5521 atttaaaact gtttgaaata taagacctgc aacagttaat atcatacctg tcaacaatgc
     5581 ttctaataat ctcggtaatc taacattgat taaaatagac tggttaaata catcattgat
     5641 atcccccaac tgccaaaata gatatatgct agttgttata atcaatatga ctatccagct
     5701 caataatact ttattaaatg tcatagcatt ctcttttctt tcatcgataa ttacattcat
     5761 tatagcaatc acatactcgc gttgctatca ttcactaaat atattttaaa accccttatc
     5821 tcaaacatgt aatttttatt acatgtagca ataaggggca taattagaat ggtttatttt
     5881 ctataaattg catctcttaa ttcttttaat ccttcatcaa tacgtggacc cggtcttgat
     5941 acttcatcac catttacaac ttcaatacgt gtattcttga cagcattaat tttattgaat
     6001 ccacctcttt ttttgatgat atccatataa tctgatcttg tcttagcttc cgtcgaaatt
     6061 aatatatctg ggttcttttt aataatactt tccttcgtta cagggttcca accattaatg
     6121 tcactataaa cattttgggc ttctaatttt tctaacatat cattgaaaaa tgtatgcttc
     6181 cctgctgtat atatttcagg ctttgatgaa acctcaataa atacttttga ttttttatga
     6241 tgagcaggaa ttgaatctat gactttatcg atattatcct tagtttcctc aacaagttgt
     6301 tcagcctgct tttcatgatg cgttaatttc ccaatttgct taaatgtgtt gtaagtttca
     6361 tcaattgatt gtgcatcttt aacatacact actttgatgc cttgtttctc taatgatgac
     6421 aatactttat tagcagttgc cttttgcgac tcatgcgcaa gaattagatc tggctttgcc
     6481 tttaaaagtt cctctttatt tagattcaaa gcatcaaatt gtttcttacc ctttttcaca
     6541 tcttttggat aatcatcaac cgttgaaaca ccaactatgt atttacctaa tcctaattca
     6601 tataaaattt cagtattact aggcattaac gaaacaattc tatggtacgg cgatttagac
     6661 gagccattat cgtcgctacc ttgtttatca ttatctttca taccacagcc actcagaaca
     6721 agcataaaaa tcaaaataaa agcaattaac gatttcttca cattatcaca tctccttttt
     6781 aaagcctctt gaactttagt gattagaggc ttttatgcag ttgtgcggtt acatccaact
     6841 gcgttccttt aatcccactt ttcgctttag cgattagagg gatttatgca gttgtgcggt
     6901 agcatccaac tgcgttcctc ttcaccttta gtcattagag gcttttatgc agttgctgca
     6961 caaacaaaca acttttttct ttatatgctt ctatttatta tataagtcca catactaaaa
     7021 gaaaatagta aattattttt tatatttcat ataaactcat ctatctaact tcacagcgtt
     7081 tactttttaa agtaaggttc taaaaaatca caattcttta tatctttatc tctaaagtaa
     7141 tgtttaccaa atacatctaa cagagcatga aattcattag catcttgatt tgtaaaatga
     7201 tttggtaatg tgactacttt ttt
//
LOCUS       DATTQF010000122         7163 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.122, whole genome shotgun sequence.
ACCESSION   DATTQF010000122 DATTQF010000000
VERSION     DATTQF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.122"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            2..421
                     /locus_tag="VB241_002144"
     CDS             2..421
                     /locus_tag="VB241_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP1245420.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            448..558
                     /locus_tag="VB241_002145"
     CDS             448..558
                     /locus_tag="VB241_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245421.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(629..4402)
                     /locus_tag="VB241_002146"
     CDS             complement(629..4402)
                     /locus_tag="VB241_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HEP1245422.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKAANKTGNAQVNQKVDTTQVNG
                     DTRATQSTTSNNAKPVTKSTNTTAPKTNNNVTSAGYSLVDDEDDNSENQINPELIKSA
                     AKPAALETQYKAAAPKATPVAPKAKTEATPKVTTFSASAQPRSAAAAPKTSLPKYKPQ
                     VNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEI
                     SYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFAR
                     SMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNY
                     SYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTPSKPSTPSTGKLTVAANNG
                     VAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWV
                     KEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQ
                     IDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQIN
                     AKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDV
                     IYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSI
                     YLYGTVNGKSGWISKAYLAVPAAPKKAVAQPKTAVKAYAVTKPQTTQTVSKIAQVKPN
                     NTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLN
                     VQNLGKEVKTTQKYTVNRSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDV
                     YLYGTINNRTGWVNSKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKY
                     SLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQIGMTLNQV
                     AQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISI
                     DKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNN
                     KVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAG
                     QNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4630..5064)
                     /locus_tag="VB241_002147"
     CDS             complement(4630..5064)
                     /locus_tag="VB241_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245423.1"
                     /translation="MFSKVIDPEMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVIKSHRGQGMGKMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5220..5690)
                     /locus_tag="VB241_002148"
     CDS             complement(5220..5690)
                     /locus_tag="VB241_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HEP1245424.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5738..6964)
                     /locus_tag="VB241_002149"
     CDS             complement(5738..6964)
                     /locus_tag="VB241_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HEP1245425.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNTDDSNNSNNNNSVNNQQQPATDQNLNQNQGG
                     TQQTPQASNNQNGVVN"
BASE COUNT         2131 a         1163 c         1188 g         2681 t
ORIGIN      
        1 catgtataaa caacttgaaa aacttattac actgactaac aatgacttaa acttagtgaa
       61 tagaagattt ggacaacgca cggatatcac atctgaacag ctagaacttc tccgtatttt
      121 atttaattac gatcgcttat cacagtatga tttgacgatg aagattagta gggaacaatc
      181 tatagtttca aggtggatta agaaattagt tttgaaaggg tacatcacaa gtcaacaatc
      241 tagcgaagat ttaagatgta aagaattaat tttgactgat caagcacgta cattaatttc
      301 acaaataaat aatgcacgtt gcgaattgat tgaagcaaga tgtcaatgtt tatcggaagt
      361 cgaattagac aatttaaatc aattacttga taagttaaat caacgacgca tatcgttgta
      421 acacggcaat agcaaacaca taatttagtg tcattgtgca cacaacaaaa attgaatcat
      481 gaattacaag caaaagtagc ggtgattgtt aaaattgatg gtaaacaatc accgctattt
      541 ttgcttgtgt atgtataaaa aagggatcaa aggtcatccc ccatgattga tagtgggggg
      601 atgacttttg atcctatgtt catgttgctt atttatattg tgggatgtcg aagtatttgc
      661 cgacttcgcc aattttatca tagtagcctt tgatgatttt agcattgatg ttagcccaat
      721 ctacatctgt agcatattgg tgtgttcctg gatgtgcagg attccatctc attttgtaaa
      781 gcgtattttg accagcttta acatatgagt tgccgatgaa tttagcacca ccaacgattg
      841 cttttgatac tgtgtcccaa ccagcttgtt tagcatattt aataccttca cgtaaaggat
      901 cgttatcata tgcagcaata ccaaatacgt tatggtattt cgtgttagag ttagttacaa
      961 ctttgttgtt cactacatct gcaccttttg ctaattgaga agtaccgtta cctgtttcta
     1021 atagggcatg tgagataaga taaacttcat taatgccata catttgagca gctttgttaa
     1081 atgcagcacc ttggttttct aatacacctt taccttttaa gaattgatta attttatcaa
     1141 tagaaatatt ttgtggttgg tctaagcgta agaattgata ttttaatgct ggatcttgag
     1201 ctaaacgctt cgtatccatt gcatgcttaa catcattaaa gttagcatct gtccacttac
     1261 ctggtacacg ttgtacttgt ggtttatatt gtaaaccagc ttgtatttga gcaacttggt
     1321 ttaatgtcat acctatttga ttatacttaa ttaattcttt agctaaatca gttgatttaa
     1381 tccatgctaa tttaccgtta gataatttac catagtacca agtttgtcca ttaatgactt
     1441 gttctttaac aactgcgaat ggttgttcat taaatgcttt taatgagtat ttagctgtat
     1501 cagaatttgg tgttacatag taataaccat taccattttt aattacataa gtgtagttat
     1561 aatctttggc agctgatgta gttggtttaa cagcagttgg tgcagttaaa tcttttgaat
     1621 ttacccaacc agtgcggtta ttaatagtac cgtataaata aacatctttg cctacagata
     1681 cttgtttcgt tgcattaaat gtaccttgag caatgttatt gcctgttaaa atgacttggt
     1741 ttttagtacc ccaaggaacc attgataagc cgttatttga tctgttaaca gtatattttt
     1801 gagtcgtttt aacttctttg cctaagtttt gaacatttaa gtcttttaca ttgaaccaac
     1861 ctaatggaat attatgactt gtattattta ataatacgta tgtttcatta ccatgtgcac
     1921 gttcttttgt tacatagaat gtacgatccg catattttgc accgtttttc gctgtttttt
     1981 cataaacaga agcacgaata ccagtgttgt ttggtttaac ttgagcaatt ttgctaactg
     2041 tttgagtcgt ttgaggttta gtaacagcat aagcttttac agcagttttt ggttgtgcta
     2101 cagctttttt aggtgcagca ggtacagcta aatatgcttt acttatccaa ccagatttac
     2161 catttacagt tccatataaa tagatagatt tatcaatttg ttgttgctta gtcgctttaa
     2221 aagtttggtt acctgtacca gataccgcac cagcttcttg tttataagta ccccaaggta
     2281 ctgaatataa tttagtgcct ggttttactg tataagtttg cattacattt acaggtgatt
     2341 ttgcattgtt ataaataacg tcaccttgtt taacccaacc aattaaagtt ggactattgt
     2401 aatctttaac taagtagaat ttgtttccac ctagacttgc ttcttttgtt acagcaaatg
     2461 ttttttgaac ttctttcgtt ggcttaccag ttttgtcata aactgtagtg aataagccat
     2521 tgtttttagc attaatttga gcaacaccgt ttaatgatga aactgttaat ttattatttg
     2581 ttgtaggtgt tgatggctta ggtgttggtg taggcgtagg tttagcagta tcaactaaat
     2641 atgctttact tacccaacca gatttaccat tcacagagcc atataaataa attgatttat
     2701 caatttgttg ttgctttgaa gccttaaatg tttggtttcc agaaccagac acgctaccag
     2761 caacttgttt agatgtaccc caaggtactg tataaagttt cgtaccaggt ttgattgaat
     2821 atgattgatt tacatttaca ggtgatttag ctgtgttgta aaccacatcg ccttctttaa
     2881 cccaaccaaa tttattacca gaattgtaat cttgaacaag atagaatttt tgattaccta
     2941 atgtagctgt tttagataca gcaaatgttt tttgaacttc attagttgct ttaccagttt
     3001 tgtcgtaaac agtagtatat aaaccactat ttgtaggttt gatttgtgcg acaccattat
     3061 tagcagcaac tgttaattta ccagttgatg gtgttgatgg tttcgacggt gttgatggtt
     3121 ttgatggtgt agtaggggta gttgtagatt gcgtacccca tggcgccact ttacccattt
     3181 ttattaaata tttttcatta attaagtcat atagttgatc ataactataa ttatgacttc
     3241 ttaaatatcc atgtggatcg gcatggtccg taccacctaa atatttactt acagcgtagt
     3301 gagtccatac tgtaccattt ccatcatatt cagcactatc aggttttaaa ccataatatt
     3361 gtaattgtgt agctgcatag tcagcatagt tattcattga acgtgcaaat gaagcataat
     3421 cgtgtgtgtg cacgatttca acattgatga atctagggtt accgactgca ccgacacccc
     3481 aagataagta atccgttggt gctgtttcga ttatacgatc cccatcaaca aatgcatgta
     3541 cgaatgcgtt ttgatagttg tttttcatat aactaatttc gccatttatc gtcgaacgat
     3601 cattagctgt atcatgaaca acgatacctt caggacgacc tacaccgtta cggtatgcgt
     3661 atttagggaa gtaagatgta taatcttcct ctatcttagg tgcttttaaa ttatttttac
     3721 gaatgtaatc attaattgat gagtttactt gcggtttata ttttggcaaa ctcgttttag
     3781 gtgctgcagc ggctgatctt ggttgtgctg aagcactaaa agtagttact tttggtgtag
     3841 cttcagtttt agctttaggt gcaacaggtg ttgcttttgg tgctgcggct ttatattgcg
     3901 tttcaagagc agcaggttta gcagctgatt taattaattc tggattaatt tgattttctg
     3961 aattatcatc ttcatcatca actaaactat atccagcact tgtaacatta ttgttcgttt
     4021 taggtgctgt tgtgtttgtt gactttgtaa caggtttcgc attatttgat gtagttgatt
     4081 gagtcgcacg agtgtcacca tttacttgtg tagtatcaac cttttgattt acttgagcat
     4141 tgcctgtttt attcgctgct tttggttgga caatagcagg gtcttgatat acttgagtgc
     4201 cagaaatgtt ttgcgttgga ttttttacct cagcttttgc ttgttcagta gttgctttaa
     4261 ctttattact atctaaaaca tttttattag tagtttgatc ttgtgtcgtc tcagctgctt
     4321 gaacttgatg tgcagtgact gctgaaccta caagcgttaa tgcaaccatt gatggtagtt
     4381 tgtaattaaa ttttttcgcc attctattta ttactcctaa catttattaa ttattaatct
     4441 cattatagta cctgttttat acacctgtgc gtatttaacc aatttgtaat atactcttaa
     4501 tttatatttt cactgtaaaa aaatgaaaaa aattttgaat ctcaatggtg acgtgacaaa
     4561 tcaaatagaa ttgatgtcaa tttaagaatt ggtttattac aaatgtttct ataatagata
     4621 caactttttt taattaagcg aggtcaactt ttttgtcatt tcaatatgct cgatgccttc
     4681 ctcaagaaat atattacctc tcattttaaa atttaaactt tcatagaatg ggattgcatg
     4741 acattgggca ttcatggtag ctacgtaaaa accttcatct ttagctaatg attctacagc
     4801 ttgcataagc attttaccca tgccttgtcc gcgatgtgat tttatcacag ccactcgttc
     4861 tattttgaca gttgtttcat tgataggacg tatacgagct gtggcaactg gctgtccatt
     4921 atcatatcca atgaggtgaa tagattcaga ttcatattca tcaatttcac tttcctctgg
     4981 gacgccttgt tcttctacaa acactttctt tcttatataa aagcaatctt ctaacatctc
     5041 tggatcaatc acttttgaaa acataaaatc cctccatatc ctaacaaaat catttacatt
     5101 taatttacta ataataacat agaataacga ttgttatttt ccacaaaaat aattagacat
     5161 aaaaaagcta taaagatgat tcataggaac catctttata gctagataga gtgtatttgt
     5221 tataatgctt cttttctaat aattgctgta tattgccaaa agattcgcat ttgagcaata
     5281 ttccaattat tcattcccat tgtaaaccca gaaggtttaa agatatttac atctgtcact
     5341 tctaaagctg ctgcgactaa atattgtaat ggaatactaa gtgatttcat tgtttcagta
     5401 atgccatttt catcatatac ccatgaaaat ggtaactctg attcgaaaac atcaacacta
     5461 ttgaaaattt caggtagggc taaaatataa catgcaccat ctacaatagg attctcgata
     5521 ctatttttgt aataaattag gagtgcttca tagttttctc gatgctcatg attgacgtaa
     5581 aaaaattcac ttctaaaatg agccatatct tggctatgaa taatttgttg ttctaacata
     5641 ttaaacatcc catttatatt tgcaatcttt tcgtatgttt ttctactcat gtatgatgct
     5701 cctttcataa atagtaaatg ttaatcgtca tataatatta gtttacaaca ccattttggt
     5761 tatttgaagc ttgtggcgtt tgttgagtgc caccttgatt ttgatttaag ttttgatctg
     5821 tagcaggttg ttgttgatta ttgacgctat tgttgttatt actattatta ctgtcatcag
     5881 tattattcga agatttatct ttatcagtgg tatcgttttg ttcttttctt aataaactac
     5941 tatctaaagg tgttaaaggt atgagagaac cataatgatt aatgacacgt tgatctaaga
     6001 aatcattttt atcattaata ggtgataatt ctaagtcttt acgaagtaag tttgcatatt
     6061 tttgaatgct ttctacactt ggatgataat agtaaatacc atttaacata tcatctttac
     6121 cttttaattg cgcagtttta atttcaacat catttgttaa gtacattttt gctaaagctt
     6181 taatttcaga gttagttaaa ttatgctttg catttttacc tacaatttga atcacgttat
     6241 caagtttatc aatagaatca acttcctgtg ctttttggaa taaaatctta attaattcca
     6301 tttgacgttg tccacgtttc aagtctgaat catgatgtct agttctagca actgctaaag
     6361 cctcatcacc atttaatttt tggtaccctt ttttaatttt aatcttacca gtatcatctg
     6421 tgtttggctc atttaaatca tatggtacat catagtatat gccaccaagt tcatttacag
     6481 cttctacaaa ggccttcata ttaacgcgta catagtaatc aacaggaaca ttcatcgttg
     6541 cttcaacaga gtccattgct gcaattggac caccatatgc atgtgcatgg gtaatcttat
     6601 cgtaatagcc gactttagga atgtagctga ttgtatcacg aggaatacta agcattcgaa
     6661 tttggtgttt agattgatta aaagtagtta aaatcatagc gtctgatcta gagtgttcag
     6721 catcttggcc tttttttctt cttccgtcat tatcatcgat acctaagaag agaatagaga
     6781 taggttgttc ttcaggattg actttattgt ctcgtaaatt tgattgacga ttagcatttt
     6841 tactatcttg agaagattca aatgcatctt gagacgtttt aaaaagtaac gtagcgaaga
     6901 caattggaac aacaataaga accaatgcta gaaggatcaa aaagtatttt aaaaatttat
     6961 tcatgattga tgctcctata ttaaattttt gttaaatcat aatcataatt ttaaaatata
     7021 attagttgaa cgatatttat acaacttcat tactaataca ttatagcctt aaattgtagt
     7081 actttatagt aaaaaataca aatgttatgt caaagttcct tgtaatttga atgttttgtt
     7141 aaaatcaaaa agcattttaa aaa
//
LOCUS       DATTQF010000123         7151 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.123, whole genome shotgun sequence.
ACCESSION   DATTQF010000123 DATTQF010000000
VERSION     DATTQF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.123"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(64..993)
                     /locus_tag="VB241_002150"
     CDS             complement(64..993)
                     /locus_tag="VB241_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEP1245426.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIVLNIFINPDVFSLHFFSNQTFNYTWFVGGLLGVGYLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIIGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSLLASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTSNIILMPHLGAALTTLIGMFGQILMGILID
                     HFALFGSPKIAMTSRKTIGLLCILTGIIFLRLF"
     gene            complement(1186..1410)
                     /locus_tag="VB241_002151"
     CDS             complement(1186..1410)
                     /locus_tag="VB241_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HEP1245427.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNIVTEQVARDSMNALVDLN
                     ILKSNSGAITKVHSAQIIDKTTTVLFEAKK"
     gene            complement(1427..1630)
                     /locus_tag="VB241_002152"
     CDS             complement(1427..1630)
                     /locus_tag="VB241_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245428.1"
                     /translation="MSKTNHITIVLSFTKLDTNGKQTEFKRRFANINPEVSNDQIKTF
                     SKLIERLTGETYNNIEVIKSVSI"
     gene            1785..2345
                     /locus_tag="VB241_002153"
     CDS             1785..2345
                     /locus_tag="VB241_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1245429.1"
                     /translation="MNKVEVIKCNDDINKMYDALKARSDRDYLFFKLAIHSGMKITEL
                     LTITVEDTKRLIEKGTLSELCKAHYHSLIKIRLPETLSNELLHYIENKSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQAAIKAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            2364..4766
                     /gene="mnhA2"
                     /locus_tag="VB241_002154"
     CDS             2364..4766
                     /gene="mnhA2"
                     /locus_tag="VB241_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HEP1245430.1"
                     /translation="MSLVYLLIAILVIMAMILLTSKRRAMAKYAGYIALTAPVIASIY
                     FLLQVPSVIKQHYLSVSIPWMTSLDINVDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYLYLTLFMFSMLGIVLADNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHALFIPMIIMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFAALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMMIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIIIVCMGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLVPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRQFEKFTTKRFKRVMQDRLNQYIIMTLGIFMVIIGYGYIRIGLPKVHQLHVS
                     EFGPLEVILAIVTVIIGLSLIFIRQRLTMVILNGVIGFVVTLFFITMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSKVNKKREIIKISVSLMMALIVVSLIFIAQQADGLAS
                     ISNFYLRADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            4753..5178
                     /gene="mnhB2"
                     /locus_tag="VB241_002155"
     CDS             4753..5178
                     /gene="mnhB2"
                     /locus_tag="VB241_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HEP1245431.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIMPTFFGKPFLSQYETTLT
                     LPILGHIHVTTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5175..5519
                     /gene="mnhC2"
                     /locus_tag="VB241_002156"
     CDS             5175..5519
                     /gene="mnhC2"
                     /locus_tag="VB241_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HEP1245432.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSNKSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5509..7005
                     /gene="mnhD2"
                     /locus_tag="VB241_002157"
     CDS             5509..7005
                     /gene="mnhD2"
                     /locus_tag="VB241_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HEP1245433.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWTAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNGLIHPLLV
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPFFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVHFKKIPQYRKG
                     ILSILVVVVIAIGIAAPVLLKITNDATELNTNDQLYQKLVNPHLKGEE"
     gene            7006..>7151
                     /gene="mnhE2"
                     /locus_tag="VB241_002158"
     CDS             7006..>7151
                     /gene="mnhE2"
                     /locus_tag="VB241_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HEP1245434.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSD"
BASE COUNT         2329 a         1015 c         1259 g         2548 t
ORIGIN      
        1 caaaaaaata gcttatcact tttaaaaata attacaagtt aaaatgataa gctaaatgtt
       61 aaattaaaat aatcttaaaa atataatccc tgtcaatata cacaacaggc ctatagtttt
      121 tcttgatgtc atagctattt taggtgaacc aaatagggca aagtgatcaa tgagtatgcc
      181 cataagtatt tgaccaaaca taccaataag tgttgttaat gctgcaccta aatggggcat
      241 taaaattata ttagatgtta caaaagccat gcctagtata ccgccagtaa aatagatagg
      301 ttttaattta ccgaatttta aatgacttgt ttttagtttt aaagaacgat taaaaatagc
      361 ggttaaaatc aatagcgtta ttgacccaat tgtaaatgat actaatgatg caaaggctgg
      421 tgaatgagta tgacttgcta ataaactgtt aattgtcgtt tgaataggtg gaaagaaacc
      481 aaaaataaat cctaagagaa gccaaaacaa taaatacttt tgatcagtta gtaataaatt
      541 attcttgtta aattgattca ttatgacgat accgataatg agtaacaata ctccaattgc
      601 tttaattaaa ttaaaatcat gaattgtagc accaaataat ccaaatgtat caataatgac
      661 acccataata atttgacccg caactgttgc aattacagtt aatgttgcac ctaattttgg
      721 caataacaat aaattgccag ttaaatagcc aacaccgagt aatccgccaa cgaaccaagt
      781 atagttgaat gtttgattac taaaaaagtg taatgagaaa acgtcaggat ttataaaaat
      841 atttaagacg attaagcata tagtacctac tgaaaatgaa ataaatgatg tatagaaagg
      901 tgatttagta tacagtgata gtcttgaatt gacagatgtt tggataggaa taagcattcc
      961 aactaggaca ccaatgatat agaaaagaag catatgaata aagtctcact ttccaaagtt
     1021 aataataaaa aacatatgta tagattatca ctatagagaa aaaatacaac ttatttttaa
     1081 aagagataaa agtaaagcga gtgccataat aataccccag tcaatcaaga agaaagactg
     1141 gggcgttaat gattatattc aatgaatgac tacctatgtt tgagactatt tctttgcttc
     1201 aaataaaacg gtcgtcgttt tatcaataat ttgagcagaa tgtactttgg tgatagcgcc
     1261 actattagac tttaaaatat ttaaatctac taatgcattc atactatcac gtgcaacttg
     1321 ctcggttacg atgttgttta attgtggtaa ttgcaatttg acaggtttgt ctaatgaaga
     1381 tttaaaactt aattcaagtg ttttggtcat gaaagtgtcc tcctgattaa attgatacag
     1441 atttgatgac ttcgatgtta ttgtatgttt ctccagtaag tcgctcgata agtttgctaa
     1501 atgttttaat ttggtcgttt gatacttcag gattaatatt ggcgaatcga cgtttaaatt
     1561 ctgtttgttt accatttgta tctagttttg taaatgataa tacaattgta atgtgatttg
     1621 ttttactcat attttaaaac ctcctttcac actatatatc gaaagaaaaa aataattggg
     1681 ttaattttat tattcttgtt taaattctat aaaaaaggtt ataattatat gtgacttaaa
     1741 tacttaatgt ttatgattaa gacacttaaa gcaaggaggg acatatgaac aaggtagaag
     1801 taattaaatg caatgatgat attaataaaa tgtatgacgc gctcaaagct cgatctgata
     1861 gggattattt gttttttaaa ttagctatac atagtggtat gaaaataaca gaattattaa
     1921 caattactgt tgaagatact aaacgactga tagaaaaggg gacactaagc gaattgtgta
     1981 aagcccatta tcactcattg attaaaatta gattgccaga aacattatct aatgagttac
     2041 ttcattatat tgaaaacaaa agtctgtcaa atgaagatgt attatttcaa tcattgagaa
     2101 ctaatcaagt attatctagg caacaagcct atcgtattat tcatcaagca gcaattaagg
     2161 ctggcataga gaatgtagga ctaacgacat tgcgtaagac atttgcgtat catgcatatc
     2221 aaaagggcat acctattcct gtcattcaaa agtatttagg gcatcaatca gctatagaaa
     2281 cattgaattt tatcggttta gaaaatgaat gtgaacacag tatttatatt tcattacaat
     2341 tataacgaaa aaggaggcta ataatgagtt tggtttatct attaattgct atactagtaa
     2401 ttatggcgat gatattatta acatctaaac gtagagcaat ggctaaatat gcaggataca
     2461 tagcgctgac tgcacctgta atagcatcta tttatttttt gttacaagtt ccatcagtaa
     2521 ttaaacagca ttatttgtct gtttccattc cgtggatgac ctcattagat attaatgtag
     2581 atttacgttt agatggtttg agtttaatgt tttcccttat tatttccctt attggaatcg
     2641 ctgtattctt ctatgcaaca caatatttat cctctcgtaa agataattta ccaaggtttt
     2701 acttatactt aacgctattt atgtttagca tgctcggtat tgtattagct gataatacaa
     2761 tattaatgta tgtgttttgg gaactaacta gcgtatcatc atttctatta atttcatatt
     2821 ggtataacaa cggagatagt caatttggtg cgatgcagtc atttatgatt acagtatttg
     2881 gtggtttggc attattagtt ggttttatca tgttgtatat catgacagga acgaataaca
     2941 tcacagagat attaggacaa gcagatcata ttaagaacca tgctttattt atccccatga
     3001 ttatcatgtt tttattaggt gcatttacaa agtctgcaca attcccattt catatttggt
     3061 taccaagggc aatggcagcg cctacacctg taagtgcata tttacattca gccactatgg
     3121 ttaaagctgg tatcttttta ttacttagat ttacaccttt gttaggtcta agcaatatgt
     3181 atatttatat cgtaaccttt gtcgggttaa taactatgtt atttggatca atcacagctt
     3241 taaaacaatg ggatttaaaa ggaatcctag catactctac aatcagtcaa cttgggatga
     3301 ttatggctat ggtgggtata ggtggcggat atgctcaaca ccaacaagac gcaatagcat
     3361 ctatttatgt atttgtgtta tttgcagcat tgttccatct tatgaatcat gcaattttca
     3421 agtgtgcgct tttcatgggt gtagggattt tagatcatga agcaggttca agggacatac
     3481 gaattttaag tggcatgcgt caattatttc caaaaatgaa tcttgttatg atgattgcgg
     3541 cgctatctat ggctggtgtt ccatttttaa atggattttt aagtaaagaa atgttcttag
     3601 atgctttaac acaaactgga caattgtccc aatttagttt gatttcaatg attatcatcg
     3661 tatgtatggg tgttattgct agtattttta cattcacata tgcactttac atggttaaag
     3721 aagtattttg gactaaatat gattctaaag tgtttactaa aaaaaatatc cacgaaccat
     3781 ggttgtttag tttaccctct cttatattaa tggtgctagt acctgtaatc ttttttgtgc
     3841 caaatatatt tgggaagggg attatcgttc cagcattaag aggtgtatca ggtggtaatc
     3901 atcaaattga tccattggta ccacatgttt cgcaatggca tggatttaac ataccgcttc
     3961 ttttaaccat catcattatt ttattgggta gtgtactagc aatcaaagta gattggaaaa
     4021 aagtgttcac aggtaaaatt agacaaattt cagtttcaaa agggtacgaa atggtttacc
     4081 gacaatttga aaagtttact acgaagcgat ttaaacgtgt tatgcaagat cgtttaaatc
     4141 aatacattat tatgacactg ggcatattta tggtaatcat cggatacggt tatattcgta
     4201 taggactacc aaaagttcat cagttacatg tttccgaatt tggaccgtta gaagttatat
     4261 tagcaattgt tacagttatc attggtttgt cattaatatt tatacgtcaa cgactaacca
     4321 tggtcatttt aaatggggtc atcggttttg ttgtaacttt attctttata acaatgaaag
     4381 cacctgattt agcattgaca caacttgtag ttgaaacgat tacgacaatt ttatttattg
     4441 ttagcttttc aagattaccc aacgtaccaa ggtctaaagt taataaaaaa cgagaaataa
     4501 ttaaaatttc agtatctctt atgatggcgc tgattgttgt gtcacttatt ttcatcgcgc
     4561 aacaagcaga tggattggca tcgatttcta atttttatct tagagcggat aaattaacag
     4621 gtggcaaaaa cattgtgaat gcgatattag gagactttag agcattagat acattgtttg
     4681 aaggtttagt attaattatt actggattag gtatttacac attattaaat taccaagata
     4741 ggaggggaca agatgaaaga gaatgatgtc gttttaagaa cggtcacgaa actcgttgta
     4801 tttattttat tgactttcgg attctacgtc ttcttcgctg gccacaataa cccaggtggt
     4861 ggctttatag gtggtttaat atttagttcc gcgtttattt taatgtttct agcttttaat
     4921 gtcgaagaag tattagaaag cttaccgata gattttagaa ttttaatgat tattggtgca
     4981 ttagtttcat ctattactgc gataatgcca acattttttg gaaaaccttt tttgtcgcaa
     5041 tatgaaacaa cattgacact accaatttta gggcacattc atgtaacgac aataacactg
     5101 tttgaattgg gcattttgtt ctcggttgtc ggggttattg taacggtgat gctgtctctt
     5161 agtggaggtc gatcatgaat ttaattttat tactagttat aggattttta gtatttatag
     5221 ggacatatat gatattatct ataaatttaa ttcgtattgt cataggtatt tcaatttata
     5281 cacatgctgg taaccttatt atcatgagta tgggcactta cggctctaat aaatcagaac
     5341 cgctgattac aggtggaaat caattgtttg tcgacccact gttgcaagct attgtattaa
     5401 ctgcaatagt tataggtttt gggatgactg cgtttttact tgtcctcgtt tatagaactt
     5461 ataaagtaac taaagaagac gaaattgaag gcttaagggg ggaagatgat gctaagtaat
     5521 ttgttaatat taccaatgtt attaccattc ctttgtgcct taatccttgt atttttaaaa
     5581 aataatgacc gtatttctaa atatttatac ttaggtacaa tgactatcac cacaattatt
     5641 tcattaatgc tattaattta tgttcagcgg catcgtccaa ttacattgga ttttggtggt
     5701 tggactgcac catttggaat acagttttta ggagattcgt taagtttaat catggttaca
     5761 accgcttcgt ttgtgattac tttaattatg gcatatggat ttgggcgtgg cgaacataaa
     5821 gcaaatcgtt atcatttacc ttcatttata ttatttttaa gtgttggtgt gataggctct
     5881 tttctaacat cagatttatt taatttatat gtcatgtttg aaattatgtt acttgcgtca
     5941 tttgtactca ttacacttgg acaatctgta gaacaattac gtgccgcaat tatttatgtt
     6001 gtcttgaata ttattggttc atggctattc ttattaggta taggtttact ttataaaaca
     6061 gtaggtacat taaacttttc acatattgca atgcgtttga atgacatggg agataatcgc
     6121 actgttacaa tgatttcatt aatcttctta gtcgctttta gtgcgaaggc agcgctggtc
     6181 ctttttatgt ggctacccaa agcatacgca gtgttaaata ctgagcttgc agcattattt
     6241 gcagcgttaa tgaccaaagt gggcgcttat gcattgattc gcttctttac attattattt
     6301 gaccaacata atggtcttat acatccattg ctagtaacaa tggcagctat aactatggtc
     6361 atcggcgcta ttggtgtcat tgcttataaa gatattaaaa agattgcagc ttaccaagtc
     6421 ataatctcaa taggatttat cattttaggt ttaggaacaa acacgtttgc aggtattaat
     6481 ggtgcaatat tttatttagt aaatgatatt gttgtaaaaa cattgctatt ctttattatt
     6541 ggtagtttag tttatattac tggttatcga caatatcaat atttaaatgg tttagcaaaa
     6601 aaagaaccat tctttggtgt tgcgtttatt ataatgattt ttgctattgg cggcgtgccc
     6661 ccatttagtg gctttccggg gaaagtactt attttccaag gtgcattgca aaatggcaac
     6721 tatattggac tagcgttaat gattattact agtctaattg ctatgtacag cttgtttaga
     6781 atacttttct atatgtattt tggagataaa gatggcgaag aagttcattt taagaaaatc
     6841 ccacaatatc gtaaaggtat tttaagtatt ttagtagttg tggttatcgc aatcgggatt
     6901 gcagcgcctg tgctgttaaa aataactaat gatgcaacag aacttaatac gaacgatcaa
     6961 ttatatcaaa agcttgttaa tccacatttg aaaggagagg aataaatgaa tcaaattgtt
     7021 ttaaatatta tcattgcatt cttatgggtt ttatttcaag atgaagatca ttttaaattc
     7081 tcgactttct tttctggata tctaattggt ttaattgtca tttatatatt acacaggttt
     7141 ttcagcgatg a
//
LOCUS       DATTQF010000124         7075 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.124, whole genome shotgun sequence.
ACCESSION   DATTQF010000124 DATTQF010000000
VERSION     DATTQF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.124"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..71)
                     /locus_tag="VB241_002159"
     CDS             complement(<1..71)
                     /locus_tag="VB241_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEP1245435.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSE"
     gene            183..1232
                     /locus_tag="VB241_002160"
     CDS             183..1232
                     /locus_tag="VB241_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1245436.1"
                     /translation="MASFTITKRKNKTSTSWQYDVKHPSFKSGKKRKSGFKTKAEASN
                     AAQQLIRDLEDGNNIEDNKKFKEYYSDWINIKNKKQLSSKQFYWYERSIKLFSEFFGE
                     NMLVKNITRSEYQRFLNKYAQGHTDETVRKVHGCLARCIKDALYDGYLKKDPTYDVNI
                     KGTEKSKDEKFKYITIKDYLNLLEYFKKRDEESYIFLYILGITGARYSDVINMTYKDL
                     NKANGIVHLPGTKTKNSKRDVEVNPRDIMRINSKLARLPRRINGKLFSVSHTSVSKSF
                     KKAKEVIGLNNDNITPYSLRHTHTSYLLSKGIPIEYISKRLGHSTISQTLNTYSHLLE
                     EHKKEQGQRVRELFS"
     gene            complement(1295..1383)
                     /locus_tag="VB241_002161"
     tRNA            complement(1295..1383)
                     /locus_tag="VB241_002161"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Ser,seq:gga)
     gene            complement(1438..1509)
                     /locus_tag="VB241_002162"
     tRNA            complement(1438..1509)
                     /locus_tag="VB241_002162"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Glu,seq:ttc)
     gene            complement(1511..1585)
                     /locus_tag="VB241_002163"
     tRNA            complement(1511..1585)
                     /locus_tag="VB241_002163"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1551..1553),aa:Asn,seq:gtt)
     gene            complement(1600..1673)
                     /locus_tag="VB241_002164"
     tRNA            complement(1600..1673)
                     /locus_tag="VB241_002164"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1639..1641),aa:Gly,seq:tcc)
     gene            complement(1689..1764)
                     /locus_tag="VB241_002165"
     tRNA            complement(1689..1764)
                     /locus_tag="VB241_002165"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1729..1731),aa:His,seq:gtg)
     gene            complement(1778..1850)
                     /locus_tag="VB241_002166"
     tRNA            complement(1778..1850)
                     /locus_tag="VB241_002166"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1815..1817),aa:Phe,seq:gaa)
     gene            complement(1867..1942)
                     /locus_tag="VB241_002167"
     tRNA            complement(1867..1942)
                     /locus_tag="VB241_002167"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1907..1909),aa:Asp,seq:gtc)
     gene            complement(1964..2037)
                     /locus_tag="VB241_002168"
     tRNA            complement(1964..2037)
                     /locus_tag="VB241_002168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2001..2003),aa:Met,seq:cat)
     gene            complement(2509..3063)
                     /locus_tag="VB241_002169"
     CDS             complement(2509..3063)
                     /locus_tag="VB241_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HEP1245437.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAN
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(3383..4783)
                     /locus_tag="VB241_002170"
     CDS             complement(3383..4783)
                     /locus_tag="VB241_002170"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEP1245438.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPFGKLRAGFDLFKKPTQMQNGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDDELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(4807..5730)
                     /gene="hemH"
                     /locus_tag="VB241_002171"
     CDS             complement(4807..5730)
                     /gene="hemH"
                     /locus_tag="VB241_002171"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEP1245439.1"
                     /translation="MTKKMGLLVVAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHKD
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(5788..6825)
                     /gene="hemE"
                     /locus_tag="VB241_002172"
     CDS             complement(5788..6825)
                     /gene="hemE"
                     /locus_tag="VB241_002172"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEP1245440.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSGFVHTYTQR"
     gene            complement(6815..6922)
                     /locus_tag="VB241_002173"
                     /pseudo
     CDS             complement(6815..6922)
                     /locus_tag="VB241_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2275 a         1385 c         1066 g         2349 t
ORIGIN      
        1 aattcactac tataattttc aactgattct tttatataag ctatttctgc gtcagtaaat
       61 tttacacaca ttttatcacc tactttttat tttattatat cacatttagt acctagtact
      121 aaaatcacgg gtagcccgcc tacccttatt attttttgcc aattttgagg aggagaaata
      181 aaatggcatc atttactatt acaaaaagaa agaataaaac gtcgacatct tggcaatacg
      241 atgtaaaaca tccgtctttc aaatcaggaa agaaaagaaa gtcgggattc aaaactaaag
      301 cagaggcaag caatgctgca caacaattga ttagagattt ggaggacgga aataacattg
      361 aagataacaa aaagtttaaa gaatactaca gtgattggat aaatataaaa aataaaaaac
      421 aattatccag caaacagttt tattggtacg aaagatcaat taagttattt agcgagtttt
      481 ttggggaaaa catgttagta aaaaatatta cgcgaagtga atatcaaagg tttttaaata
      541 aatacgcaca aggtcatacc gacgagaccg tgagaaaggt tcacggttgt ttagcaagat
      601 gtatcaaaga cgcattatat gatggttatt taaaaaaaga ccctacctat gatgttaata
      661 tcaaaggcac tgaaaaatct aaagatgaga agtttaaata tataacgatc aaagactact
      721 taaacttact agagtacttt aaaaaaagag atgaagaaag ctatatcttt ctgtatattt
      781 taggtattac tggtgcaaga tatagcgatg ttattaatat gacatacaaa gatttaaaca
      841 aagcaaatgg tatagttcat ttacccggaa caaaaacaaa gaattcaaaa cgagacgtag
      901 aagttaatcc aagagatatt atgcgtataa attcaaagtt ggccagattg ccgcgtagaa
      961 ttaacgggaa gctattttcg gttagtcata catcagtgag caaatcattt aaaaaagcaa
     1021 aagaagtaat aggattaaat aatgataaca tcactcccta ttcacttaga catactcaca
     1081 catcttactt actatcaaaa ggcataccga ttgaatatat tagcaaacga ttaggtcact
     1141 ctacaatatc gcaaacgtta aacacctatt cacacctact tgaagaacat aaaaaagagc
     1201 aaggtcaacg agtcagagaa ttattttctt gacacctatt tgacacttgc tagaatgaaa
     1261 agcttttaaa tcaaggtttt aaagcttatt tttacggaga gtgagggatt cgaaccctcg
     1321 agacgcttgt ggcgcctaca cactttccag gcgtgctcct tcggccaact cggacaactc
     1381 tcctcaacaa taaatatgta ataaaaaaac agaagcgata tttcacttct gtttgtatga
     1441 ctcctacggg actcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac
     1501 caaggagcca tggctccaca ggtaggactc gaacctacga ccgatcggtt aacagccgat
     1561 agctctacca ctgagctact gtggaataat agaaaataat ggagcgggtg atgggaatcg
     1621 aacccacaac atcagcttgg aaggctgagg ttttgccatt aaactacacc cgctaatgta
     1681 aacgattatg gggcggctga aggggatcga accctcgaat gtcggaacca caatccgatg
     1741 tgttaaccac ttcaccacaa ccgccatgaa ttaagaatgg ttcaggacag agtcgaactg
     1801 ccgacacatg gagcttcaat ccattgctct accaactgag ctactgaacc gtaatggtaa
     1861 taataatggc ggtctcgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcgtg
     1921 ttaaccgcta cactacgaga cctattaaat taaaaactat gtattgcggg aggcggattt
     1981 gaaccaccga ccttcgggtt atgagcccga cgagctaccg aactgctcca tcccgcgcta
     2041 atattatttt gaattaccta attaatatac cataatcaaa aacctaaagt caagaacttt
     2101 ttgaatttaa tttaaatgtt atctcttaaa taattactta aatatcgtag caacatgttc
     2161 tctgttgaac acaaatatta gtatattcat ttttgtagta tacgtcaacg acatttttaa
     2221 agttttttgt gtaaaaaaac gcttcttatt cccttttatc atataagtgt ctaatagttg
     2281 ttataaatag tgttacagca ttttaaaagg tataggaatt ataaagttta caacgcctat
     2341 accttctgaa aaagaaattt aaaagcttgc tacaaccgca tgctcattca gtcaacgact
     2401 accaatataa cattgtagcc ctaagacatt ccttggcgct tcaattacca catttttttc
     2461 aaaatcagca gctacctaca gacacaacat aacacaacaa tacattacct atcgtgtcat
     2521 gtaatcttgc atccgatctt gcaacgctgt aaatgtttcg aagccatcct cttctaagaa
     2581 gtgccctcca tcttccacga ttcgcaagtt cccctctaat gcattcatta aacgctgagt
     2641 ctctttatat gaaacgtatt tgtcattttt agaactcaat ccgtaaaaat tgtcaacttt
     2701 ctttttaata ttatcgtaat caatagttac attacttaaa tcgatatcta aatctatatt
     2761 ttctgcatct tctttaaagc ccgctatact gaaaaagcct tcaatcggct gatcaatcat
     2821 ttcaatatat tttaaagctg tgattgaacc taaaccatgt gttacaaaat atgtatcctt
     2881 tttgcgtaca ttaatttgtt tcgtcatagc ttcaatccac tgatccactg tattcgcttc
     2941 aggggattca aaattaaata atgttacgtc atatccttct aaagttaagt tatgctccaa
     3001 ccactgatac caatgatttc tactatttcc atgcatagaa tgtacaatga ttacatctgt
     3061 catctcattc tctcctttca acttactact tcttttctat ttaaaaaaat gaatgattac
     3121 ctataattgt aaaataaaaa caccttaatt agaaatgtta tatcgcaaag tgacatttct
     3181 aattaaggtg cattgtcatc atttcaatat cattcaataa tagctaaacc ttcttcttcg
     3241 attcagtttc atgaaataat tcccgctgaa agtatcatga tttacacatt acttccacca
     3301 ttatataact taaaaatgac tatatttcat caaacattat ctaaaggcgt cgcacctaca
     3361 ccaactccat ccaacaatta acttacaact ctgcgattac ttcttcagca gcaactttac
     3421 cttgtgtaat acaatcaggt agtccaaccg cttcaaaaga tgcaccagtt actctaagtc
     3481 gtggatatgt ttgtttaata tgtgcttgaa tctgcctaat ttgttgaatg tgaccaacat
     3541 ggtactgtgg catacttttc ggcaaacgat tgacaattgt aaattcagga tcgcctttaa
     3601 atgtcatcat ttgacttaaa tctctacgta caatcgatac taattcatca tctgtatgat
     3661 catcaaccac agtatcacct ggtttaccta catacgcacg aatcaaaacc ttaccttctg
     3721 gtgtagtaaa tggccatttt ttcgatgtcc aagtacatgc ggtaatgtca gtatcactcg
     3781 ttctcgcaat cacgaagcca gtaccatcat aagtattttc aatgtctttt tcatcaaatg
     3841 ccaatacaac agttgcaaca gtcgtactat ccatcgtttt aaaataatca aatgctggat
     3901 cttgtccgaa ccaattcaaa aaaacttgat gcggtgttgt cactaatacc ccatcgaata
     3961 catcttcttg ttgattactg taaacaattt tatattgctt ttgagatgta ataatatcat
     4021 ccactgacgt attgtaacgt attgtcacac ctttattttt cacatcttgt tctaatgctt
     4081 caataaatga acttaaacca tgcttaaatt gtttgaattg tccttttggt gcgccaggat
     4141 ataattgtct ttgtttcaga cgcttatttt tctcatcctt catacctttt atcagacttc
     4201 cgaatgcctc ttctttttct ttaaaattag gaaacgtact catcaaactt aatttatcaa
     4261 tatcggtacc ataaatacca cccattaaag gctcaattaa attctcaagt acctcattac
     4321 ctaatcttgc tctgaaaaat gcaccaacag aaatgtcacc attctgcatt tgagtaggtt
     4381 ttttaaataa atcaaaccct gctcttaact ttccaaatgg tgaaattaat ttagttgtca
     4441 caaacggttt gatatctgtc ggaattccca taattgatcc acctggaata ggatacaatt
     4501 tgtttttcgc aaaaatataa gattgtccag tcgtatttgt aacaatatct tgttctaatc
     4561 caatatcttt cgctaattct gtcataatcg tttttctacc taaataagat tcaggcccta
     4621 gttcaatcat ataaccatct ttacgatacg attgaatctt cccccccgga cgattcgatg
     4681 cttcaaagat ggttacatca atattaggat cttgctgttt taaaaaatat gcacttgata
     4741 aacctgttat ccccgctcct ataatagcca ctgatttagt cacaacgttt cacgcttctt
     4801 tcgtcattaa aatatagact tgatttcatc aacaattgca ccgataaata atggatgtgt
     4861 attcggcatt tttggacgat aataattcgc gccaatgtca tcgcaaacaa ctttacattc
     4921 ataatcattg tcataaagca cctctaaatg ctcacataca aaacctactg gcgtatatat
     4981 aaagttttta tactgatgtt tttcatataa atcacgtgtt aaatcttgta catctggccc
     5041 taaccaaggt gtacctgtat taccttcaga ttgccaacca atcgcgatat gttcaatatt
     5101 agattgttct ttaattaaaa gcgcagtatg ttctagttct tgtggatatg gatcattatt
     5161 cttttcgatt aaaccttttg gcaaactatg tgccgaaaca actaataccg tgtctttgtg
     5221 ttcctcttcc ggtatttgag ctaatgtttc gttgacttta ttcgtccaat attcaataaa
     5281 tttaggttgt tcataataat gtttcacatg tgtaagttga ataccatatt ttgcagcttc
     5341 ttcatcagca cgtttgtcat atgatcctac tgaaaatgaa gaataatgtg gtgctagtac
     5401 tacagtgatt gcttcagtaa taccatcttt gtgcatttgt tcaaccgcat cttcgataaa
     5461 tggtgaaatg tgttttaatc ctaagtatag tttaaattca acatctgcat atgctttatt
     5521 taatgctgaa actagcgcat cagcctggtc atctgttgta cctgctaatg gtgataaacc
     5581 acctataaat tcatatctat ctttcaaatc ttgaagttct tcttcagatg gacgtttacc
     5641 atgtctaatg tctgtataat atggctctat gtcactttct ttataaggtg tgccataagc
     5701 cacaactaat aatcccattt ttttagtcat tgataatacc ttcctttaaa tgaattatct
     5761 ttcatgtgct tcaatgtaat actatgatta tctttgtgta tatgtgtgta cgaatccgct
     5821 tactttacgt aacgtctctg gttgcacttc tgggaaaaca ccgtgtccta aattaaagat
     5881 gtgtttaccg ttctccatac cttgatctaa tattggtttc aatctctctt caatgacatt
     5941 ccatggtgct aataaaattg atggatctaa attaccttgt aatgttttag taacgcctaa
     6001 ttgttgagcc tgattaatag acgttctcca atctaggcct aatacatcaa tcggcaaatc
     6061 attccattca ttgattaaat gactggcacc tacaccgaat agaattaccg gcacatcatg
     6121 tttttcttta acctcactga ttaatcgaat catatgtggt ttaatgtaac gtctgtaatc
     6181 ctcgacattt aatgcaccta cccatgaatc gaaaatttga atcaattcgg cacctgcttc
     6241 gacttgagct gttacatatt taacagatac atcgactaaa tgattcatta aagcaaacca
     6301 tgttgcttca tctctataca tcatcgcttt tgtaaaattg taatttttcg atggtccgcc
     6361 ttcaatcata tatgacgcta atgtaaatgg tgccccagta aatcctatta gcggcacatt
     6421 taacttttct tctgttaaaa gtttaattgt atctaataca tatggtacat ctcgttcggg
     6481 gtctatttga gaaagtttct caacatcttg aattgttttg ataggattat gaatcactgg
     6541 accaataccc gatttaattt ctacatcgac accaattggc tttaatggtg tcataatatc
     6601 tttgtataaa attgctgcat ctgtatgata attatcaact ggtaaatgtg taacataagc
     6661 gcacaactcc ggctgatgtg taatatcgaa tagtgaatat ttttctttca attttcgata
     6721 ttctggttgc gaacggccag cttgtcgcat aaaccaaaca ggtgtatgtg tcgtttcttc
     6781 acctttaatc attttcaaaa ttgtattgtt tttattatgc accataaagg cctcctaaat
     6841 taaaatcatt cttatctata ttatcatatc gctcattcgt tcgtattttc aataaataaa
     6901 tgtcataaaa ctgacattaa attacacatc atttgtttgt gtatttaaat tttaaaatct
     6961 tgatggatta tctccacatt tttttgtctt gcatgattta ttatagtaac tttaaccgta
     7021 tattaatagt ggtaatgact agtttatcgt cgtataataa ataaaaacat aaggg
//
LOCUS       DATTQF010000125         6875 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.125, whole genome shotgun sequence.
ACCESSION   DATTQF010000125 DATTQF010000000
VERSION     DATTQF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.125"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            126..635
                     /locus_tag="VB241_002174"
     CDS             126..635
                     /locus_tag="VB241_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HEP1245441.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(748..1938)
                     /locus_tag="VB241_002175"
     CDS             complement(748..1938)
                     /locus_tag="VB241_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245442.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(1916..3091)
                     /locus_tag="VB241_002176"
     CDS             complement(1916..3091)
                     /locus_tag="VB241_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HEP1245443.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICQDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            3523..5007
                     /locus_tag="VB241_002177"
     CDS             3523..5007
                     /locus_tag="VB241_002177"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP1245444.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLSANVTQVVVPDTLRVASILAHTLFDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESL
                     QGVSFDFVTPFGTYQVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            4988..5248
                     /locus_tag="VB241_002178"
     CDS             4988..5248
                     /locus_tag="VB241_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HEP1245445.1"
                     /translation="MALLVNKRLLDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LNEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            5248..6810
                     /locus_tag="VB241_002179"
     CDS             5248..6810
                     /locus_tag="VB241_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP1245446.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRLILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
BASE COUNT         2427 a         1008 c         1256 g         2184 t
ORIGIN      
        1 tttactgttt aatttaattt aaatttgtcg aaattgcaaa agatgtataa tgaattattt
       61 ttaatgtaac ggttttcaaa gaaatttgat ataatagcaa taggttaaac aaaggaggaa
      121 ttcagatgat tttaggatta gcattaattc catcaaagtc atttcaagaa gcggtggatt
      181 cttaccgtaa aagatatgat aaacagtatt cacgaattaa accacatgtg acaattaaag
      241 caccatttga aattgaagat ggtgatttag attctgtcat tgaacaggtt agagctcgta
      301 ttaatggtat accagcagta gaagttcatg ctacaaaagc ttctagcttc aaaccaacga
      361 acaatgtgat ttactttaaa gttgcgaaga cggacgactt agaagaattg tttaatcgct
      421 ttaatggaga agatttctat ggagaagctg aacatgtttt tgtgccacac tttacaatag
      481 cacaaggact atctagccaa gaattcgaag atatttttgg tcaagtagca ttagctgggg
      541 tagaccataa agaaattatc gatgaattaa ctttgttacg ttttgacgat gacgaagata
      601 aatggaaagt tattgaaacg tttaaattag cttaagtaac ataatagtat tgttaatcgt
      661 agtatgtttg aattaataag aaaatggtca tttttattga atgtaataaa aatgaccatt
      721 ttctttattt taaaatacgt tttaacctta cttagctttt tctctatttg ctataaagta
      781 gcttccataa aatacagcta agactaaaaa gattaatgcc gagaaataaa atgtattgtt
      841 taaattgttg gtaaattgtg taattaatcc gccaaataat ggcccgatca ttgagccgaa
      901 tccttggata ctattaaaaa caccccaagt ttcttcttgt tcatctgatt tgataaatcg
      961 tgccataaag gtattccatg ctggtaataa gatgccatac attagaccga tagctaaagc
     1021 gataatccac acgatgtgaa tattaacaat catagataga gtaaaaatta atatcatgta
     1081 taaaataaat ccgcttagaa taacaccata cataaagttt ctgctgcggt tatctattag
     1141 tttcgataaa aatagcatcg aaactgcaca gccgatacca ccaataatga ttgcaacagt
     1201 atattcaatt gtgcttacgt taataacctt agtagcatat gttggtaata taggaactag
     1261 tgcagcaatt gcggcacctt gtaaaagaat accagggaac aacaataaat ggcgctttgt
     1321 cacatcaaca atttgtctca attgagcttt aactggacga gtattataat ttgttaactt
     1381 tacatcgaca aaataatata atatccaagc aattaaaacg actaaagaca tcatgaaggc
     1441 aaagcgtgtt gggtgcactt tgataagtag attcataaaa accataccta ccaataggcc
     1501 taacaaccat gaaaaataaa catagcccat ttgtttgcca cgtttatctt cttcaacact
     1561 ggataacata atgacccaaa taggactaac tgcaataccg agcatcatag cactaaatat
     1621 gattacaaaa ggtgatgctg gaaaccaaat aactaaaaat aaacttgtaa atgctaaaat
     1681 aaatccagtc gttaaaacga tttttgtgcc gaattttttc agtaaaaatc ctataacaaa
     1741 gtttgtagat gcatcagcaa taaaatgtat tgaaaatgct agagacgtta ttgctacagc
     1801 aatggatgtt actgttggca agaaattaat atagcttagg atatacatgc ctctcgcaaa
     1861 ttccattaaa aataagataa taagcattaa aatgaaattt ttatgattag cgtaattatt
     1921 taacgaagaa tcttgcatat aaaggaacct ttccataaat ctcttgtggt tgtgatgaat
     1981 gaccgattaa atcaagtaag tcttgacata ttgtctgtgt agcatactta attttatctt
     2041 gttccattgt actaatcatg ttagttaatt gctcattacc attcgttaaa ctagctacaa
     2101 tttttattgc ttcttctgga gtatcagcga ttttaccaaa acctttttct tcaaagtaaa
     2161 gggcattttc aagctcttga ccaggtgcag gatttaggaa aatcattgga atacaacggg
     2221 cgaaaccttc agttattgtg ataccaccag gtttcgtaat cataagttga cttgatgcca
     2281 tccattcatt catgtgtttg gtataaccta gaatcaatac attctcgtta gatttaaact
     2341 tagctgttaa agaacgcttt agctctttgc tcttaccaca aatcataact acttgtgcat
     2401 ttgcactttt cgctaatata tcagtgatca tcgtgtcaaa gcctttagat acaccaaatg
     2461 caccagctga cattaaaata gtttgcttat ctggatctaa gttgttgtct attaaccact
     2521 gcttttgatt aataggtgtt tcaaatttgt tatcaatagg aatacctgtc actttaactg
     2581 ttgaaggatc aatacctacg tctataaagt cttgtttcgt ttcttttgtt gccacataat
     2641 atcttgttga atacggcgta atccagtttt tatgtaagcg atagtctgtc atcactgtag
     2701 caactggaat attaatgtta aattgctcag ttagtaccga cataactggt gtaggaaacg
     2761 ttaataatat taaatccggc ttttctttta tcaataaatt tattaattta ttaagtccat
     2821 agtatttgta gaaacatttg tctagtttat ctgggcggct gtaataaaac cctttgtaca
     2881 tatttctaaa atatttaaag ctattgatat accatttttt acaaatagaa gtcaaaattg
     2941 ggtgagcttc cataaataaa tcgtgctcaa tgacgcttaa atggtctaga ttcatatcat
     3001 taagttgatt aacgatactc tgtgtaactt gcatatgacc gttaccgaaa gagccagtaa
     3061 taatcaatat ctttttattt tgagtaacca ttaatagcca ccctccgtta gtttgaaaat
     3121 tttatttaag tgtaacttat tttacggcat tataaaagaa ataaagacgc aaagtcgtta
     3181 catttatagc attttaatca atagatgaat tgatacaaaa taaaacgtta ttttattaag
     3241 caatttattg ttctgtgttt tatttgtata tttaaaatta tccagtatac aattatagca
     3301 tatttttgga aacaattatg atattatacc atgttacaag atggttttaa taatttaaga
     3361 tgaaccataa ttgtgaaact aattcataat accttatgtt ttatttttaa tagtagaaat
     3421 tagaaaatgc tgattagtag gatataacag tgaaattata aatttattaa catcaacaaa
     3481 acgtgtataa taaacatatt gtagaaaaag gagcggttca gtttggatgc aagtacgttg
     3541 tttaagaaag taaaagtaaa gcgtgtattg ggttctttag aacaacaaat agatgatatc
     3601 actactgatt cacgtacagc gagagaaggt agcatttttg tcgcttcagt tggatatact
     3661 gtagacagtc ataagttctg tcaaagtgta gctgatcaag ggtgtaagtt ggtagtggtc
     3721 aataaagaac aatcattatc agctaacgta acacaagtgg ttgtgccgga cacattaaga
     3781 gtagctagta ttctagcaca cacattattt gattatccga gtcatcagtt agtgacattt
     3841 ggtgtaacgg gtacaaatgg taaaacctct attgcgacga tgattcattt aattcaaaga
     3901 aagttacaaa aaaatagtgc atatttagga actaatggtt tccaaattaa tgaaacaaag
     3961 acaaaaggtg caaatacgac accagaaaca gtttctttga ctaagaaaat taaagaagca
     4021 gttgatgcag gcgctgaatc catgacatta gaagtatcga gccatggctt agtattagga
     4081 cgactgcgag gcgttgaatt tgacgttgca atattttcaa atttaacaca agaccattta
     4141 gattttcatg gcacaatgga agcgtacgga cacgcgaagt ctttattgtt tagtcaatta
     4201 ggtgaagatt tgtcgaaaga aaagtatgtc gtgttaaaca atgacgattc attttctgag
     4261 tatttaagaa cagtgacgcc ttatgaagta tttagttatg gaattgatga ggaagcccaa
     4321 tttatggctg aaaatattca agaatcttta caaggtgtca gctttgattt tgtaacgcct
     4381 tttggaactt accaagtaaa atcgccttat gttggtaagt ttaatatttc taatattatg
     4441 gcagcaatga ttgcggtgtg gagtaaaggt acatctttag aaacgattat taaagctgtt
     4501 gaaaatttag aacctgttga aggacgatta gaagttttag atccttcgtt acctattgat
     4561 ttaattatcg attatgcaca tacagctgat ggtatgaaca aattaatcga tgcagtacag
     4621 ccttttgtaa agcaaaagtt gatattttta gttggtatgg caggcgaacg tgatttaact
     4681 aaaacgcctg aaatggggcg agttgcctgt cgtgcagatt atgtcatttt cacaccggat
     4741 aatccggcaa atgatgaccc gaaaatgtta acagcagaat tagccaaagg tgcaacacat
     4801 caaaactata tagaatttga tgatcgtgca gaagggataa aacatgcaat tgacatagct
     4861 gagcctgggg atactgtcgt tttagcatca aaaggaagag aaccatatca aatcatgcca
     4921 ggacatatta aggtgccaca tcgagatgat ttaattggcc ttgaagcagc ttacaaaaag
     4981 ttcggtggtg gccctgttgg tcaataaaag acttcttgat gaaggtaaaa ctattgatgt
     5041 ttatttattc gaagcattaa atgaccagat aatcattgct ataccagatt ggttttggtc
     5101 atatcagatg gcaatgacat taaatgaaga aacttgtttt gaagcaatac tcatgcaatt
     5161 gtttgttttt aaagaagagg aagaggcaga atcgattgca tcacaactaa cagattggat
     5221 agaaacatat aaaaaggaga aagactaatg aacttaaagc aagaagttga gtctagaaag
     5281 acttttgcga ttatttcaca tcccgatgca gggaaaacaa cgttaactga aaaactattg
     5341 tacttcagtg gtgccattcg tgaagcgggt acagttaaag ggaagaagac tggtaaattt
     5401 gcgacaagtg actggatgaa agttgaacaa gagcgtggta tttctgtaac aagttcagta
     5461 atgcaatttg attacgatga ttataaaatc aatatcttag atacaccagg acatgaagac
     5521 ttttcagaag atacgtatag aacattaatg gcagttgaca gtgctgtcat ggtcatagac
     5581 tgtgcaaaag gtattgaacc acaaaccttg aagttattta aagtttgtaa aatgcgtggt
     5641 attccaattt ttacattcat taataaatta gaccgagtag gtaaagaacc atttgaatta
     5701 ttagatgaaa tcgaagagac attaaatatt gaaacatacc ctatgaattg gccaattggt
     5761 atgggacaaa gtttctttgg catcattgat agaaagtcta aaacaattga accatttaga
     5821 gatgaagaaa atatattaca tttgaatgat gattttgagt tggaagaaga tcatgcaatt
     5881 acaaatgata gtgcttttga acaagcaatt gaagaattaa tgttggttga agaagcgggt
     5941 gaagcctttg ataatgacgc gctgttgagt ggagacttaa cacctgtatt tttcggttca
     6001 gctttagcta actttggtgt acaaaatttc ttgaatgcat atgttgattt tgcgccaatg
     6061 ccaaatgcga gacaaacaaa agaagaggtt gaagtaagcc cgtttgatga ttcattttca
     6121 ggatttatct ttaaaattca agccaacatg gaccctaaac accgtgatag aattgccttt
     6181 atgcgtgtcg ttagtggtgc atttgaacgt ggtatggatg tcactttgca acgtactaat
     6241 aaaaagcaaa agatcacacg ttcaacgtca tttatggcag acgataaaga aactgtgaat
     6301 catgctgtag caggcgatat cattggacta tatgatacag gtaattatca aattggagat
     6361 actttagttg gtggaaaaca aacctacagt ttccaagatt taccacaatt tacgccagaa
     6421 atttttatga aagtttctgc taaaaacgtc atgaaacaga agcatttcca taaaggtatt
     6481 gaacaattag ttcaagaagg tgcgattcaa tactataaaa cattacacac aaaccaaatt
     6541 attttaggtg ctgttggtca gttacaattt gaagttttcg aacatagaat gaaaaacgaa
     6601 tataatgttg atgttgttat ggagccagta ggccgtaaaa ttgcacgttg gattgaaaat
     6661 gaagaccaaa ttacagataa gatgaacaca tcaagattga ttttagtgaa agatagatat
     6721 gacgatttag tattcttatt tgaaaatgaa tttgcaacaa gatggttcga agagaaattc
     6781 cctgaaatta aattgtatag tttactttaa cggctcaatt gtataatcga atttgttaca
     6841 ttaaaaataa ttgtttcctt gaagaaaaat aaatt
//
LOCUS       DATTQF010000126         6850 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.126, whole genome shotgun sequence.
ACCESSION   DATTQF010000126 DATTQF010000000
VERSION     DATTQF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.126"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..380
                     /locus_tag="VB241_002180"
     CDS             <1..380
                     /locus_tag="VB241_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP1245447.1"
                     /translation="EIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEVFNF
                     LKNQKNIIWLDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFD
                     SHLLSISEIYYELLNLIKASDQY"
     regulatory      367..464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            539..1630
                     /gene="gcvT"
                     /locus_tag="VB241_002181"
     CDS             539..1630
                     /gene="gcvT"
                     /locus_tag="VB241_002181"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEP1245448.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGAIVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1650..2996
                     /gene="gcvPA"
                     /locus_tag="VB241_002182"
     CDS             1650..2996
                     /gene="gcvPA"
                     /locus_tag="VB241_002182"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEP1245449.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AESEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDDTAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2989..4461
                     /gene="gcvPB"
                     /locus_tag="VB241_002183"
     CDS             2989..4461
                     /gene="gcvPB"
                     /locus_tag="VB241_002183"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEP1245450.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPISDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPYTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(4846..5232)
                     /locus_tag="VB241_002184"
     CDS             complement(4846..5232)
                     /locus_tag="VB241_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP1245451.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5390..6220
                     /locus_tag="VB241_002185"
     CDS             5390..6220
                     /locus_tag="VB241_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP1245452.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEVQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6284..6502)
                     /locus_tag="VB241_002186"
     CDS             complement(6284..6502)
                     /locus_tag="VB241_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245453.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDTFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6516..>6850)
                     /locus_tag="VB241_002187"
     CDS             complement(6516..>6850)
                     /locus_tag="VB241_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245454.1"
                     /translation="STYYDKVAKALKSYNDIEKDVSKNKGDKNVQSKLNQISNDIQSA
                     HTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNHAFDDIKNGYQNKDKKQLTKGQQALS
                     KLNLNAKS"
BASE COUNT         2411 a          933 c         1272 g         2234 t
ORIGIN      
        1 cagaaatatt tagtaaacat ggtgaacaat atttcaggaa tttagagttc acatgtttgc
       61 aagaatgtat taacactgca gatataattg ctactggtgg tggtattatt gaaagtgaag
      121 aggtatttaa ttttttgaaa aatcaaaaaa acattatttg gttagattgt aatattgata
      181 ttatatatag tcgaatcaat gatgacccac atcgacctaa tgcaaataat aagacaatca
      241 agcagttaaa tgacttgtat tgctcgcgga ttttaagata taatgaaatc gcattcaaga
      301 aatttgatag tcatttgcta tcaatttcag aaatatatta tgaattgcta aatttaataa
      361 aagcgagtga tcagtattag agagaataga gcgttaagac tctatcgccg aaggtgcaag
      421 taatttatta cgaaactctc aggcaaaagg ataatactgt aacgcgttcc tgaattggtg
      481 atttataaac agggtagcga ttgctatcct gtttttataa ttttaagggg gtatttcaat
      541 gtcaagtgat ttaaaacaaa cacctttata tcaaaattat gttgatagag gtgcaaaaat
      601 tgtggaattc ggaggatggg cgatgcctgt tcaattttca agtattaaag aggagcataa
      661 tgctgttcga tacgaaattg gcctgtttga tgttagtcat atgggtgaaa ttgaagtgac
      721 aggtaaagat gctagtcagt ttgtgcaata tttattatca aatgatactg ataatttaac
      781 tacttcaaaa gcattatata ctgctttatg taatgaagaa ggcggtatta ttgatgattt
      841 agtaatatat aaattagctg acgacaatta tttattagtt gttaatgctg ctaatactga
      901 aaaagatttt aattggattt taaaacacaa agagaaattt gatgttgaag tacaaaatgt
      961 atcaaaccaa tatggtcaat tagcaataca aggaccaaaa gctagagatt taattaatca
     1021 attagttgat gaagatgtaa ctgaaatgaa aatgtttgaa tttaaacagg gtgtcaaatt
     1081 atttggagcg atcgtcattt tatctcagtc aggttacaca ggtgaagatg ggtttgaaat
     1141 ttattgtaat atcgatgata ctgaaaaaat ttgggatggt ttattagagt ataatgttat
     1201 gccatgtggg ttaggcgctc gtgatacatt aagattagag gctggattgc cattacatgg
     1261 acaagattta actgaatcaa ttacaccata tgaaggtggt atcgcttttg caagtaaacc
     1321 attaattgat gctgatttta ttggtaaatc tgtattaaaa gatcaaaagg aaaatggtgc
     1381 accaagaaga acagtgggat tagaattact tgaaaaagga attgcaagaa ctggttatga
     1441 agttatggat ttagatggaa atattattgg agaagtaact tcaggaacac agtctccatc
     1501 atcaggaaaa tcaattgcac ttgcaatgat aaaaagagat gagtttgaaa tgggtagaga
     1561 gttgcttgtt caagttcgta agcgtcaatt aaaagcgaaa attgttaaga aaaatcaaat
     1621 tgataaataa ttaaaaaggg gtgtgcattg tgagtcatcg ttatatacct ttaactgaaa
     1681 aagacaagca agaaatgtta caaacaattg gtgcaaaatc tataggagaa ttattcggtg
     1741 atgtaccaag tgacatttta ttaaatagag atttaaatat tgctgaaagc gaagcggaaa
     1801 caacgttact tagaagatta aatcgcattg caagcaagaa tatcactaaa gaaacgcata
     1861 catcattttt gggtgctggt gtttatgatc attacgcgcc atcagttgtg gatgctatga
     1921 tttcaagatc agaattttac acagcatata cgccatatca accggaaatt tctcaaggtg
     1981 aattacaagc catttttgag ttccaaactt taatttgtga attaacagat atggatgtag
     2041 caaattcttc tatgtatgat gggatgacta gctttgctga agcatgtatt ttagcattta
     2101 gtcaaacaaa gaaaaataaa attgtagtct caaaaggttt gcattatcaa gcattacaag
     2161 tacttcatac ttatgctaag acgcgtaagg aattcgaagt agtggaaatc gatttagatg
     2221 gcactgttac agatttgaaa aaattagaag cagcagtgga tgatgataca gcagctgttg
     2281 cagttcaata tccaaacttc tatggttcaa ttgaagatct tgaaaagatt catagcttta
     2341 ttgaagataa aaaagcatta tttatcgtat atgcaaatcc attagcattg ggattactta
     2401 cacctccagg ttcatttggt gcagatattg ttgtaggtga tactcagcca tttggaattc
     2461 ctgctcaatt cggtgggcca cattgtggat attttgcaac gacgaaaaag ttaatgcgta
     2521 aagtaccagg tagattagtc ggtcaaacac aagatgatga aggtaatcgt ggctttgtat
     2581 taacattaca agcacgtgaa caacatattc gacgcgataa agcgacatct aatatttgtt
     2641 ctaaccaagc attaaatgcc ctagcgtcat caattgcaat gtcagcactt ggtaaacaag
     2701 gtatctatga tattgcagtt caaaatatag aacatgctaa ctatgctaaa caacaattta
     2761 ttaaaaaagg ttttgaagta ttagatggta catctttcaa tgagtttgta gtcaaatttg
     2821 acaaaccaat tcaacaagtt aacgaggaat tagtgaagta caatattatc ggtggttttg
     2881 atttaggtgt tgtttcagac gactttaaaa atcacatgtt aatagctgta actgaattaa
     2941 gaacaaaaga tgaaatcgat acatttgtag aaaaggctgg tgagttaaat gactagtaaa
     3001 tcaagtccat taatttttga aagatctcgt gaaggcagat atgcatattc attaccaata
     3061 agtgatatta aaacaaattc tgttgagtca ttgttagatg ataaatttat tcgtaaaaac
     3121 aaagcggagt ttcctgaagt tgctgaacta gacttagtac gtcattatac tgaactttca
     3181 aataaaaatt tcggtgtaga taacggattt tatccattag gttcatgtac gatgaagtat
     3241 aaccctaaaa ttaatgaaaa ggttgctaga ataccaggat tcagtgaatc acatccatta
     3301 caagatgaag accaagttca aggttcatta gaaattattt atagtttaca agaagaatta
     3361 aaagaaatta ctggtatgga tgaggtaaca ttacaaccag ctgctggtgc acatggtgaa
     3421 tggactgcat tgatgatatt taaagcttat catgagaata atggtgaagg tcatcgtgat
     3481 gaagtcattg tgccagattc tgcgcatggt acgaatccag cctcagcttc atttgcagga
     3541 tttaaatcag ttactgtaaa atcaaacgaa cgtggcgaag ttgatattga tgacttgaaa
     3601 cgtgttgtaa atgaaaatac agcagctatt atgttaacta atccaaacac tttaggtatt
     3661 ttcgaaaaaa atattatgga aatccgtgaa atcgtccata atgctggtgg tctattatat
     3721 tatgatggtg cgaatttaaa cgctattatg gacaaagttc gcccaggaga tatgggattt
     3781 gatgctgttc atttaaactt gcataaaaca tttactggtc cacatggtgg cggcggtcct
     3841 ggttcaggtc cagtcggtgt agtaaaagaa ctagcaagtt acttaccaaa gccaatggtt
     3901 attaaagatg gcgacaaatt taaatatgat aatgacatta aaaattctat cggacgtgta
     3961 aaaccatttt atggtaactt tggtatttac ttaagagctt atacgtatat tcgaactatg
     4021 ggagcaactg gccttaaaga ggtttccgaa gcagcggttc ttaatgcgaa ttatattaaa
     4081 gcacgtttat ctgaacactt tgaaatacct tataaacaat attgtaaaca cgagtttgtg
     4141 ttaagtggtg ttcgtcaaaa agaatttggt gtacgtactt tagacatggc taagcgatta
     4201 ttagatttcg gtgtacatcc accaacaatc tacttcccat taaatgttga agaaggtatg
     4261 atgattgaac cgactgagac agagtctaaa gaaacacttg attattttat cgatacatta
     4321 attagtattg ctgaagaagc taaaaatgat cctgataaag tgttagaagc accatataca
     4381 actgtgattg atcgattaga cgaagctaca gctgctcgta aaccaatatt aaagtttgaa
     4441 aatcttaaac aggaaaaata aggtgttaaa cacatattcc gagaattatt attctaactt
     4501 tgtatgaaga tttaaggata atggttttaa aatcaattaa aaaagacaat ttctatttga
     4561 aaaagaaaac taaaccgaag taataactct tagggtttgg tattattctt tcatagaaat
     4621 tgtcttttca ctttttagat tgaggtaatt gaatcgtatt gaaaatgagt tgaactctct
     4681 ttattatgca gaaactaaat ttaatgatct gtgaagctag agaaatatag taacagtctt
     4741 tgttaaaaaa ttaaaatcct ttgttaaaaa attaaaatct gagtattgtt attgatgatc
     4801 aattaacact cagattttat taaatcgtat attaaattta caaaactatt ttttagactt
     4861 tatttttcca gtccattttt tatagccgcc ttttaacata tagatatctg tatatccatt
     4921 ctttttcaaa atacgagcgg ctctatagct agcaatccca ttggcatcac ataagtatac
     4981 cggttggtct tttcttaatc cttggaatcg ttgcctgaac attgtcatag gaatattgcg
     5041 agacccatta atgtgaccgt agtcatagtc aactttctct ctaacatcga tgacttgagc
     5101 ttttctaatc ccattatgga attcattttg atctaattct ttaacagctc gcttgttaag
     5161 aatttgttga acaatcatat aagcaataat tgctataact aaaattattg cgatgtacaa
     5221 actagcactc atcttgcatc ctccctaatt aaacgataat ataattataa gactgttagg
     5281 tcaaattatc aaaatatttc actaaagaat tgcaattatt attcatttag ttaaattgat
     5341 taattagcga atcaaatatt atacattttg ctgataggag ttcttgaata tgactgaaac
     5401 ttggaatttt attaatacag gaagtaaaga cccatattat aatatggcaa tggatgaagc
     5461 gttattaaat tttgtctcta gaggtgaaat tgatccagtt atacgttttt acacatggaa
     5521 tcctgcaaca ttatcaatag gttatttcca aaggctacaa aaagaaattg atattgataa
     5581 ggttaaagag aaaggttttg gtctcgtaag gcgtcaaact ggtggccgtg gggtgcttca
     5641 tgataaagag ctaacttaca gtgtgatagt gcctgaatcg catcccaata tgccttctac
     5701 tgttacagaa gcgtacagag tgatttcaca aggattatta gaaggattta aaaacttagg
     5761 atttgatact tattttgcgg tacctaaaac gcctgaagag cgtcaaaaat taaaacaacc
     5821 tcgaagttca gtgtgttttg atgcaccaag ttggtatgag ctagttgttg aaggccgtaa
     5881 gattgctggg agtgctcaaa cgagacaaaa gggcgtaatc ctccaacacg gttcgatttt
     5941 acaagatatt gatattgacg aattgtttga tatgtttatt tataaaaatg aaagattaaa
     6001 attaaaaatg aaagaggcat tcgttgaaaa agcagttgcc atcaatgata tttcagatga
     6061 acacattact attagtcaaa tggaagaagc tttcgagaaa ggttttaaga aaggtttaaa
     6121 tatagaatta aagccacttg aattaacaga agttcaattg gcagaagtgg aagaattaac
     6181 tgaaaaatat cgttcagatg aatggatgtt tagaaagtag tttttagaat aaaaaatgga
     6241 agcacgtaga ctattttaac tacaagtgct tccatttttt tatctatttt cttctttgat
     6301 ttcttttata cttacgcatt gctttgcgat atttgcgttg gtcttcagtt aagatgaaga
     6361 aataataaat cagataaata ataccagcta ttacagcaaa gcttactaac attctgacga
     6421 ttgaaaaaat aaaggtatct agattcatta ctaatccaat cgcagcaatt ataagtacaa
     6481 ggtaaaatat tatattacgc attaaaagac tcctatcatg attttgcatt taagtttaat
     6541 tttgacaacg cttgttgtcc ttttgtaagt tgttttttat ctttattttg atagccattt
     6601 ttaatatcat caaatgcatg attcaaatca gagtttaatt tatcgatatt tttagacgtt
     6661 tttttatcat catcactaag tgataaacca tcgatagcat ctttgtatga agtgtgagca
     6721 ctttgaatat cattagaaat ttgatttaat ttcgattgaa cattcttatc gcctttgttt
     6781 ttacttacat ccttttcaat atcgttatag gatttcaaag ctttagcaac tttgtcgtag
     6841 tatgttgatg
//
LOCUS       DATTQF010000127         6800 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.127, whole genome shotgun sequence.
ACCESSION   DATTQF010000127 DATTQF010000000
VERSION     DATTQF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.127"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            137..1075
                     /locus_tag="VB241_002188"
     CDS             137..1075
                     /locus_tag="VB241_002188"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP1245455.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNTANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKNYGISRLDSISKKHFKNREKAQKILKPL"
     gene            complement(1377..3779)
                     /locus_tag="VB241_002189"
     CDS             complement(1377..3779)
                     /locus_tag="VB241_002189"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP1245456.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIFVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQVRLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVIGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3779..4837)
                     /gene="pheS"
                     /locus_tag="VB241_002190"
     CDS             complement(3779..4837)
                     /gene="pheS"
                     /locus_tag="VB241_002190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP1245457.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5218..5958)
                     /locus_tag="VB241_002191"
     CDS             complement(5218..5958)
                     /locus_tag="VB241_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP1245458.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(6198..6305)
                     /locus_tag="VB241_002192"
     CDS             complement(6198..6305)
                     /locus_tag="VB241_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245459.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(6342..6665)
                     /gene="isdG"
                     /locus_tag="VB241_002193"
     CDS             complement(6342..6665)
                     /gene="isdG"
                     /locus_tag="VB241_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HEP1245460.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(6684..>6800)
                     /locus_tag="VB241_002194"
     CDS             complement(6684..>6800)
                     /locus_tag="VB241_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB family sortase"
                     /protein_id="HEP1245461.1"
                     /translation="SDVNVTVKDKIMTLSTCEDAYSETTKRIVVVAKIIKVS"
BASE COUNT         2186 a         1310 c          935 g         2369 t
ORIGIN      
        1 catgaccact atgtattttg taagtatccg caattaattc tttacaacat atataaatgt
       61 ttctgacgtt attatcattt atgatatgat tatttttgct aatgataatg aaattattac
      121 gaaaggattt acacaaatgg cgaatatcgt ttttaaattg tcggataaag acataacgac
      181 attaatgtca cgcatttctt ttgatactga gaatttacct caaggtatga aagcacgtgc
      241 aaagtatcaa aatacaactg taaatattta ccaatcaggt aaagtcatgt ttcaaggtaa
      301 tcatgcagaa gctgtgtctg aagaacttct accgcagcat tctcaattga atactaataa
      361 aactaaaaag aaaaatacgg ccaattcttc tcttgaacag actttaatgt atgatcaatt
      421 taactgtatt ggtagtgatg aggctggaag tggcgattac tttggtccgt tgactgtgtg
      481 tgctgctttt gttaccaaag agcatgtccc tattttaaaa actttgggtg tagatgattc
      541 aaaaaaatta accgatacta aaattgtaga acttgctgag caattggtta ctttcatccc
      601 acattcacta ttaacactac acaatgaaaa atataatatt cagcaagcaa aaggctggac
      661 tcaagtaaaa atgaaagctg ttcttcataa tgaagccatc aaaaatgtac ttgaaaaaat
      721 tgattcgtct caattagatt atatcgtcat tgatcagttc gcaaaacgcg aagtgtatag
      781 ccattacgca ttatctgata taccacttcc aaagaaaaca aaatttgaaa caaaaggaga
      841 atcgaagtca ttagccattg cagtggcaag cataatttct agatacgcat ttattacata
      901 tatggatcaa atatcaaaaa atattaatat gacgattcct aaaggtgctg gtgctaaagt
      961 cgatgtaatc gccgccaaaa taataaaaaa ttacggtatt tcacgtttag attccatttc
     1021 aaaaaaacat ttcaaaaatc gtgaaaaagc ccaaaaaatt ttaaagcctc tttgatttat
     1081 aatcattaga ggcttttatg cagttgtgca aagcatcaac tgtaatccgc tgagcctcta
     1141 tgatttataa tcaagataaa ttggatccta ataggaaatc ctattaggta agtcatacta
     1201 atttgctttg ttcataagta tgactttata tgcagttgtg caaagcatca actgaaaacc
     1261 actcttcctc tttgatttat aaatgaaatg gaacctaata ggtgaatcca aaaataaaac
     1321 cccatgttag tgatatcaat ctacttcaga tatccttata catggggttt aaatcattat
     1381 ctaataacag caccttgttc aattaatgct gcttcaattt ccgcttgtac ttttgaaacg
     1441 cgttcatctg tcaatgtttc ttctgtgtct aaataattta aacgtattgc aattgatttt
     1501 ttaccttttt ctaaatgttc gccctgatat acatcaaaga caagtgtatc ttttaatata
     1561 ttgccaccgt gtgcatgaat cgttgataat aaatcagctg ctggaatatt ttgatctact
     1621 tctaatgcaa tgtcacgaga catgcctggg aatctcggaa ttggctggta attaatgtaa
     1681 cctaccgaca cagccattaa tgcatcaaaa ttcaactcaa aaacatacgt acgttttaaa
     1741 tcattatcag ctgctaatgt tgggtgtaat tcaccaataa aaccaataac tttattctct
     1801 aataagattt cagctgtacg acctggatgt aatccatcaa tatcagcacg acgataacta
     1861 aattcaaggt ttaacttttc agatactcga tccacgacac cttttgctaa ataaaaatca
     1921 accgtttctt tcttaccttg ccattgattg actacataat ctccagttaa aataccactt
     1981 aaatattcaa cttgatctgg tagttcacct tcaccattag caaagaagac attgccgatt
     2041 tcaaataatt ttacatcttt atttttacgt gccacattat atgatgccgc ttcgattaaa
     2101 tgtggtaata aactttgacg taatgacgca tgcgcttcac tcattggcat caataaatca
     2161 attgtttgac gctgttgcat cgcaaatgca gtagcatctt ctttagaaac taacgaatac
     2221 gtaatagctt ggtctaatcc agcaccttct aacacttctt taaccattct cgttttatat
     2281 tggcgatcag ttaactgacc actagtaact ttttcgaaga caggtaatgt tgaaggaata
     2341 tcatcgtaac cataaatacg tgcaacttct tcaattaaat cttctttaat tgtaatatct
     2401 ttgcgacgtg acggcacttg cactgtaata acatcatcat ttatttctgt atcaaaccct
     2461 aattgattaa aaatagtaac aatatcattt tgtgacaaat caaatccaat agtgcgatta
     2521 attttatcag cagtgatgtc gattggtgta ataaatgcac caagttctcc tgaagacact
     2581 ctatctttta gcacttttcc gtttgcataa gtttgtaata aataacatgc acggtcgact
     2641 gcttcatcta caaattcagt agctattcct ttttcaaaac gactagatga ttcactgcgt
     2701 aaatttaaac gtcttgatgt atgacgaatt gaaactggat caaaaatagc accttcaata
     2761 actatatttg atgtatgttc tttaacttct gaaaaatcgc cacccataac accagctaat
     2821 gcaattggag tttgtccatt agtaataaca atatcgctcg ttaataattc acgttctgta
     2881 tcatctaatg ttgtcatttt ttcgccttca ttagcttgac gaacaacaat ctgttgtgaa
     2941 ccaatcgcat cttgatcaaa catgtgcaat ggttgaccgt attctaataa cacataattt
     3001 gagatgtcga caacattatt aataggacgt atacctgctt ttattaagcg tacttgcatc
     3061 caaattggcg aaggttcaat tgtcacgtcg tgaacaacac gtgcactata atatggtact
     3121 ttatcttcat tttcaattgt cacagtcagt tcatcatttg cagataactc aagctcattt
     3181 gatgttgtct ctggcttagt catttttgta ttatataatg ctgcaacttc ataagcagta
     3241 cctatcatac ttaaagcatc agcacgattc ggcgttaaat caaattccat tacttgatca
     3301 tctaaatata aagcttgtaa ggcatctgtt cctggaactt gggattcact aaatacaaaa
     3361 atgcctgatt caaaactttt cggtatatag ttacttgaaa taccaatttc ttgtaacgaa
     3421 caaatcatac cttccgaacg ttcaccgcgt aatttggcac gcttaatttt aataccacca
     3481 ggcaatctac cacctacttt agcaacaatg acatattgtc ctgcatcaac gttcggggca
     3541 ccacatacga tttgtacagg ttcgtcttct ccgatatcaa cttggcaaac atttaattta
     3601 tcagcatcag gatgtttctc ttttgactta acgaagccaa caactaaatt tttgatatct
     3661 tttgtgtagt caattaaatc atccacttca atacctgtgc gcgtaatacg ttctgccaaa
     3721 ttacttacag aatcatcgat tgttacatat tctttcaacc attcatttga tatcaacatt
     3781 acatgtcacc tctatcttct accgctttaa attgatctaa aaatctcaca tcattagtat
     3841 agaaatgacg aatatcttct ataccatatt tcaacattgc aatacggtct ggtcccatac
     3901 caaatgcaaa tccagagtac tctgaagaat caaaaccagc catttctaat acattaggat
     3961 gtaccattcc agcgcctaaa atttcaatcc atcctgtgtg tttacacaca ttacaacctt
     4021 ttcctttaca tttaaaacat gacacatcaa cttctacaga aggttcagtg aatgggaagt
     4081 aacttggacg taaacgaatt tcacgatcag caccaaataa tttcttagct aacaattcta
     4141 aagtaccttt caaatcactc attttaacgt ttttatcaac aactaatcct tcaatttgtg
     4201 taaattgatg actatgtgtc gcatcatcag agtcacgacg atacacttta ccagggcaaa
     4261 taattttaac tggaccttga ccatgacgtg attccatcgt acgtgcttgc actggtgatg
     4321 tatgcgtacg taataaaatt tcatccgtaa tatagaaact atcttgcata tcacgtgcag
     4381 ggtgtgattt aggtaaattc agcatttcga agttataatg atcttgttca acttcatatc
     4441 cattgacaat ttcataacct aaacctaaga ataagtcttc aatttcttct attgtacgtg
     4501 ttaatggatg ctttgaaccg atttcaatat gacgacctgg taatgataca tcaattgttt
     4561 cttcagccaa ttgcttattt aatttttctt taactaacat ctgttgtctt tcatctaatt
     4621 cattttgaat tgtttgacgc aattcattca ctttttgacc aaacgcaggc ttctcttcat
     4681 tcggcaaatc cttcatcaat ttcattagtc cgctaactga ccctttttta cctaagtatt
     4741 tcactttaac ttcttgcagt gcacgttcat catttgcttc attaatatct acaagcgctt
     4801 gttgttttaa ctctgacatt gtttgttgtt cagacatatg tcactgacct ccctattcga
     4861 actattcatt ttctatttat catatttgtt gtttaactaa aaaaagactt tcatcctctt
     4921 aaaaaataag ggacgaaagt catttccgtg gtaccaccca aatttatgcc taagcataca
     4981 cttgtttaat attgataacg gattgtcttt ccgacttaaa tctctttaag taaccaaaaa
     5041 ggtgaaaaga ctttaaataa ttggaataac ttgcagtcac gattattctc cctaaaacaa
     5101 ttatttatat aaatgtccat gcctttttat tggtttatat tcaattgtgt atgcaatcac
     5161 gtagaaatgt tctgttaaac aattaattat aattatatcg gaaaactttc aacacggtca
     5221 acctttcaaa tgataaagta aaatactacc tgcaatcgct acatttaaac tttcagcttt
     5281 accataaata ggtatgatta aattttgtgt agtatgtgct aataattcag gattaacacc
     5341 ttctccctca ttacctaata gtaatgcaaa agaatcactt gaagtaactt ctttatatgc
     5401 cactgcgttt tcaagtgctg taccataaac aggaccatta aactgagtaa taaacgtatc
     5461 gagatcttgt gtcataactg gcaaatgaaa aacactacct tgactcgctc gcaacacttt
     5521 atcttgataa ggatctgtcg tacccttttc cattattaca gcatccattc cagcagcatc
     5581 cgcagttcta attaatgtgc ctaaatttcc aggatcttga acacgatcga taagcaatac
     5641 ttgttgtgct ttagaaatat catattgtgg cttctcaatg attgcgaaaa acccttgtgg
     5701 tgtcactgta cctgataaag attcagcaac tttcatgttt atttcaaaaa cttcttgcgc
     5761 gtatgcgata atttgctgat ctaatcttgc cggttcaatt gcaaataatt gtgtaattac
     5821 aatcccactt tgataagctt cttcaattaa atgcacacct tcaattaaag ctaatccagt
     5881 tttatccctc tcacgtttct tttttagctt gttcgcttgt ttaattctat tattttgtgc
     5941 agaagtaatt tgttccatag atagctcctc gctttatttt taaaaataaa aatattaatc
     6001 attaataaga tgaaaacatt tgattgtata gttaatatta attaatcgat tttatcactc
     6061 atattatttc aaattgtata aatttctttt atcgatacta ctactataaa tcatacgccc
     6121 caaaatatca ttattaattc ttttcttctt caaaataaat caaaatcata tatttgatga
     6181 ttattttcaa agcacattca tatcaaacta cgttttagca atttgttgtt agcatgtttg
     6241 tgttcattaa aaaaacgacc atcatcggta tcatgtatgg tcgttacaaa agctaacaat
     6301 accaattgtc ataacaagta ctgcaacctt tttaaattca attatttcat gtaactatag
     6361 cctatatcat atgtaattac tttgttatta ataatcgggc tactttcatc ttcattttta
     6421 cttctaacat gtttatgtgc tgctttaaag acatcagatt ttaaccaatc cgtaaaagct
     6481 tgctttgatt tccaaactgt taaaattttc acttcatcaa aatcttcttg ttctaaagtt
     6541 tgtgtaacaa acatgccatc aaagccttct aatgtttcaa tcccatgtct cgtgtaaaat
     6601 cgttctataa tatcttttgc tgttcctttt gttaacgtca gcctattttc tgccataaat
     6661 ttcataatta tcctcttttc tgtttaactt accttaatta tttttgcgac aacaacaatt
     6721 cttttcgttg tttcactata tgcatcttcg cacgttgata aagtcattat tttatctttt
     6781 accgttacat taacatctga
//
LOCUS       DATTQF010000128         6766 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.128, whole genome shotgun sequence.
ACCESSION   DATTQF010000128 DATTQF010000000
VERSION     DATTQF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.128"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            161..1132
                     /locus_tag="VB241_002195"
     CDS             161..1132
                     /locus_tag="VB241_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245462.1"
                     /translation="MKYLLKGKILLLLLILLTIISLFIGVSELSIKDIFHLSDSQQNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSISGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            1119..2075
                     /locus_tag="VB241_002196"
     CDS             1119..2075
                     /locus_tag="VB241_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEP1245463.1"
                     /translation="MRNNNKKIMLLIAVTFLISMLYLFVGIDFDIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVIILVITIFLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            2072..2833
                     /locus_tag="VB241_002197"
     CDS             2072..2833
                     /locus_tag="VB241_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245464.1"
                     /translation="MIQVGNLTKTINNQMILDDISIEIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHEIVNDALDLLQLQDIRKRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVAMKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIRNQRICLYFKD"
     gene            2948..3976
                     /locus_tag="VB241_002198"
     CDS             2948..3976
                     /locus_tag="VB241_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP1245465.1"
                     /translation="MKKTVLYLVVAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLISSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKKFNKS
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKNVSNSNHGQNVSNEYINKENPDV
                     ILAMDRGQAVSGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     DELEKVVK"
     gene            complement(4293..4607)
                     /locus_tag="VB241_002199"
     CDS             complement(4293..4607)
                     /locus_tag="VB241_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245466.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(4625..5548)
                     /locus_tag="VB241_002200"
     CDS             complement(4625..5548)
                     /locus_tag="VB241_002200"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP1245467.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNVEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLVKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(5675..6193)
                     /locus_tag="VB241_002201"
     CDS             complement(5675..6193)
                     /locus_tag="VB241_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEP1245468.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     TNHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDRLLSTNDEIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            6313..>6766
                     /locus_tag="VB241_002202"
     CDS             6313..>6766
                     /locus_tag="VB241_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HEP1245469.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFE"
BASE COUNT         2393 a          942 c         1139 g         2292 t
ORIGIN      
        1 atttacaaaa aatgatttct attttaattg caaatatcaa taaaattgac aataaattat
       61 ttgaaaggct attgaaatga gtgacaaaaa acgataccat taatgagaaa tgttatcaat
      121 gataataatt atcattaaat taaagggaga aaattttgta atgaagtatt tattaaaggg
      181 aaaaattttg cttctattac ttatattgct aacaattatt tcgttgttta taggtgtcag
      241 tgaactgtct attaaagata tctttcattt gtcagactca cagcaaaata tattgttctc
      301 aagccgaata ccgagaacga tgagtatttt aattgctgga agttcattag ctttagctgg
      361 cctaatcatg caacaaatga tgcaaaataa gtttgttagt ccaactacag ctgggacgat
      421 ggagtgggca aaattaggaa tcttaattgc cctattattc tttccaactg gtcatatttt
      481 attaaaacta gtatttgctg ttatttgcag catcagcgga acatttttat ttgttaaaat
      541 tattgatttt atcaaagtga aagatgtcat ctttgtacca ctattaggga ttatgatggg
      601 tggcattgtc gctagtttta caacgtttat ctcattacgt acaaacgcag ttcaaagcat
      661 tggtaactgg cttaacggga actttgccat tatcacaagt ggacgttatg aaatcttgta
      721 tttaagtatt cctcttttag cattgacata tctttttgca aatcatttca caattgtagg
      781 tatgggtaaa gactttacca ataatttagg tttgagttat gaaaaattaa tcaatattgc
      841 tctatttatt actgccacaa ttacggcatt agtcgttgtg acagttggaa ctttaccctt
      901 cttaggttta gttattccaa atattatttc gatttataga ggcgatcatt taaaaaatgc
      961 aattcctcat acgatgatgt taggtgccat ctttgtattg ttttctgata tagttggcag
     1021 aattgtagtt tatccatatg aaattaatat tggtttaaca ataggtgtat ttggaacaat
     1081 cattttcctt atcttactta tgaaaggtag gaaaaattat gcgcaacaat aataaaaaaa
     1141 taatgctttt aattgcagtc acgttcttaa ttagtatgct gtacttattt gttggtattg
     1201 attttgatat atttgaatat caattttcaa gtcgtttaag aaagttcata ttaattattt
     1261 tagtaggtgc tgccattgca acatccgttg ttattttcca agcgattaca aataatcgtt
     1321 tattgacacc atcaataatg ggattagatg cagtttattt atttatcaaa gtattgcccg
     1381 tctttttatt tggaattcaa tcggtatggg ttactaatgt atatttgaac tttatattaa
     1441 cacttataac gatggtgtta ttcgcactaa tcctattcca aggtatcttt aaaattggac
     1501 atttttcaat ttactttatc ttacttatag gtgtcctttt aggaacattt tttagaagta
     1561 ttacaggttt tattcaattg attatggatc ctgagtcatt tttagcaata caaagtagta
     1621 tgtttgctaa ttttaatgct tctaattcga atttagttac tttttcagca gtattattag
     1681 taatcatatt agtcattaca atttttctat tgccatattt agatgtattg cttttaggtc
     1741 gtgctgaagc aattaatctg gggatatcgt atgaaaaatt aacgcggatt ctacttgtaa
     1801 tagtttctgt cttagtctct gtgtcaactg cattagtagg accaattaca tttttaggct
     1861 tattgactgt aaatctagcg catgaactaa tgaagacgta tgaacataaa tatatattaa
     1921 tcgcgacaat ttgcttgagt tggattagtt tatttagtgc gcaatgggta gttgaaaatg
     1981 tgtttgaggc tacgacagaa atgagtatac ttattgattt aattggtgga agttatttca
     2041 tttatctatt agttagaagg agaaatgcgc aatgattcaa gttgggaatt taactaaaac
     2101 tataaataat caaatgatat tggacgatat tagcatagaa attgaaaaag gtaagttgac
     2161 ttctttaatt ggacctaatg gagcagggaa gagtacgtta ctttcagcga tatgtcgctt
     2221 aattcggttt gatgaagggg aagtaaaaat agatggacag cttatgtctg attataaaaa
     2281 caatgatttg tcgaaaaaga tatcgatttt aaagcaaacg aaccatacag aaatgaacat
     2341 tactgtagaa caattagtta atttcggacg atttccatat tctaaaggac gtttaacgaa
     2401 agaagaccat gaaattgtta atgatgcttt agatttgttg caattacaag atattagaaa
     2461 gcgtaatata aaatcattgt ctggtggaca acgtcaacgt gcctatatag cgatgaccat
     2521 tgcacaagat actgaatata ttcttttaga tgaaccgtta aataatttag atatgaagca
     2581 tgctgttcaa attatgcaaa cgttaaaaat gttagcgcat aaaatgaata aagcgattgt
     2641 cattgtgtta catgatatta actttgcgtc ctgttattca gatcagattg tagcaatgaa
     2701 aaacggacaa ctagttaaat cagatttgaa agataatgtc attcaaagta gtgttttaag
     2761 tgatttatat gacatggata ttcaaattga acatataaga aatcaaagga tttgtttata
     2821 ttttaaggat tgataatttg gagacacttt aaaggggtga tgcgccaatt aaagaagggt
     2881 taacaagtat ttattcgtat ttcacatcaa gcacacagat taagccaaaa gaggagaata
     2941 ttatattatg aagaaaacag tcttatattt agtagtagca gtaatgtttt tattagcggc
     3001 atgcggtaac aattctgata aagaacaatc aaaatcagaa actaaaggtt ctaaagatac
     3061 agtaaaaatt gaaaacaact ataaaatgcg tggcgagaaa aaagatggta gcgatgctaa
     3121 aaaagttaaa gaaactgttg aagtaccaaa gaaccctaaa aatgcagttg tattagatta
     3181 tggcgcatta gatgtaatga aagaaatggg cttatcagac aaagtaaaag cattacctaa
     3241 aggtgaaggc ggcaagtcat taccgaattt cttagagtca tttaaagatg acaagtatac
     3301 aaatgttggt aacttgaaag aagttaattt cgataaaatt gcagcaacaa aaccggaagt
     3361 aatctttatc tctggccgta cagcaaatca aaagaattta gatgaattta aaaaagctgc
     3421 accaaaagca aaaattgttt acgttggtgc agatgaaaag aatttaatta gttcaatgaa
     3481 acaaaacact gaaaatattg gtaaaattta cgataaagaa gataaagcta aagaattaaa
     3541 taaagattta gataacaaaa ttgcttcaat gaaagataaa actaagaagt tcaacaaatc
     3601 agttatgtat ttattagtta atgaaggtga attatcaaca tttggaccta aaggtcgttt
     3661 cggtggatta gtttacgata cattaggttt caacgcggtt gataaaaacg taagcaatag
     3721 taaccacgga caaaatgtct ctaacgaata cattaataaa gaaaatccag atgttatttt
     3781 agcaatggat agaggacagg ctgtaagtgg taaatcaact gcgaaacaag cattaaataa
     3841 tcctgtatta aaaaatgtta aagcaattaa agaagacaaa gtttataatt tagaccctaa
     3901 attatggtac tttgcagctg gatcaactac aactacaatt aaacaaattg atgaacttga
     3961 aaaagttgta aaataatttt aaaagagggg aacaatggtt aaaggtctta atcattgttc
     4021 ccctctttgc tttaaaaaag ggaatctggg acgtcaatca atgtcctaga ctctaaaatg
     4081 ttctgttgtc agtcgttggt tgaatgaaca tatacttgta acaagctcat ttcaatacct
     4141 gtgggctcca aacatagaga aattggattt ccaatttcta cagacaatgt aagttgccgg
     4201 gctccaaaca tagagaatat caaaaagaaa ttctacagaa gtggtgcttt atcatgtcta
     4261 tcccactccc tataattttt tggctatgtt gtttaaattt cgaaataaat atgatagtga
     4321 tatttgcagc gattgttaaa tcgagattgg caattaggac aacgctcaac catcatatat
     4381 tcattgattg ttaattcatg tttgcataca ccgcataaga ttgctttttc gttaaatgaa
     4441 ggctcagacc aacgcttaat ggcgtgcttt tcaaactcat tatggcactt atagcatgga
     4501 tagtatttat tacaacattt aaatttaatt gcaataatat cttcttcggt aaaataatgg
     4561 cgacagcgtg tttcagtatc gattaatgaa ccataaactt taggcataga caaagctcct
     4621 taacttacga ttcctttgga tgttcaccaa taatgcgaac ttcacgattt aattcaatgc
     4681 caaatttttc tttgacggtc ttttgtacat aatgaataag gttttcataa tctgtagcag
     4741 ttccattgtc tacatttacc ataaaaccag cgtgtttagt tgagacttca acgccgccaa
     4801 tacggtgacc ttgcaaatta gaatcttgta tcaatttacc tgcaaaatga ccaggcggtc
     4861 tctggaatac actaccacat gaagggtact ctaaaggttg tttagattct ctacgttctg
     4921 ttaaatcatc cattttagct tgtatttcag tcattttacc aggagctaaa gtaaatgcag
     4981 cttctaatac aactaagtgt tctttttgaa taatgctatt acgataatct aattctaatt
     5041 cttttgttgt aagtttaact aacgagcctt gttcgtttac gcaaagcgca tagtctatac
     5101 aatctttaac ttcgccacca taagcgccag cattcatata cacagcacca ccaattgaac
     5161 ctggaatacc acatgcaaat tcaaggccag taagtgcgta atcacgagca acacgtgaga
     5221 catcaataat tgcagcgccg ctaccggcta taattgcatc atcagatact tcgatatgat
     5281 ctagtgataa taaactaatt acaatgccac gaataccacc ttcacggata ataatatttg
     5341 agccattgcc taaatatgta acaggaatct cattttgata ggcatattta acaactgctt
     5401 gtacttctac atttttagta ggggtaatat aaaagtcggc attaccacct gtcttagtat
     5461 aagtgtatcg ttttaaaggt tcatcaactt taattttttc atttgggata agttgttgta
     5521 aagcttgata gatgtcttta tttatcactt ctcagtacat cctttctcat gtctttaata
     5581 tcatatagta ttataccaat tttaaaattc atttgcgaaa attgaaaaga aagtattaga
     5641 attagtataa atataaaata cggcattatt gtcgttataa gtatttttta catagttttt
     5701 caaagtattg ttgcttttgc atctcatatt gtctaattgt taagctatgt tgcaatattt
     5761 gatgcttttt agtattgaat tgtaaagcga tctcatcatt agtcgataag agacgatcaa
     5821 gtgcaagata agattcaaat gtttgggtat tcatttgaat gatatgtaga cgcacctgtt
     5881 gttttagttc atgaaaattg ttaaacttcg ccatcataac ctttttatta tatttatgat
     5941 gcaaacgata aaaccctaca taatttaagc gtttttcatc taaggatgta atatcatgca
     6001 aattttctac acctactaaa atatctaaaa ttggctctgt tgaatattta aaatgattcg
     6061 taccgccaat atgttttgta tattttactg ggctgtctaa gaggttgaat aataatgatt
     6121 caatttcagt gtattgtgat tgaaaacaat tagttaaatc actattaatg aatggttgaa
     6181 catttgaata catgataaac tcctttgata ttgaaaatta atttaatcac gataaagtct
     6241 ggaatactat aacataattc attttcataa taaacatgtt tttgtataat gaatctgtta
     6301 aggagtgcaa tcatgaaaaa aattgttatt atcgctgttt tagcgatttt atttgtagta
     6361 ataagtgctt gtggtaataa agaaaaagag gcacaacatc aatttactaa gcaatttaaa
     6421 gatgttgagc aaaaacaaaa agaattacaa catgtcatgg ataatataca tttgaaagaa
     6481 attgatcatc taagtaaaac tgatacaact gataaaaata gtaaagaatt taaggcacta
     6541 caagaagatg ttaaaaacca tctcatacct aaatttgaag catattataa gtcagcaaaa
     6601 aatttgcctg atgatacaat gaaagttaag aaattaaaaa aagaatatat gacgcttgca
     6661 aatgagaaga aggatgcgat atatcaatta aaaaaattca taggtttatg taatcaatct
     6721 atcaagtata acgaagacat tttagattat acgaaacaat ttgaaa
//
LOCUS       DATTQF010000129         6752 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.129, whole genome shotgun sequence.
ACCESSION   DATTQF010000129 DATTQF010000000
VERSION     DATTQF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.129"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            181..384
                     /locus_tag="VB241_002203"
     CDS             181..384
                     /locus_tag="VB241_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HEP1245470.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(381..617)
                     /locus_tag="VB241_002204"
     CDS             complement(381..617)
                     /locus_tag="VB241_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245471.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            842..1423
                     /locus_tag="VB241_002205"
     CDS             842..1423
                     /locus_tag="VB241_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP1245472.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1496..2113)
                     /locus_tag="VB241_002206"
     CDS             complement(1496..2113)
                     /locus_tag="VB241_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEP1245473.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLFIIIAVVGVSIIIMSLPILQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2269..2763)
                     /locus_tag="VB241_002207"
     CDS             complement(2269..2763)
                     /locus_tag="VB241_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245474.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3162..3584)
                     /locus_tag="VB241_002208"
     CDS             complement(3162..3584)
                     /locus_tag="VB241_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEP1245475.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDPDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3732..4448
                     /locus_tag="VB241_002209"
     CDS             3732..4448
                     /locus_tag="VB241_002209"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP1245476.1"
                     /translation="MTHVEVVATITPQLYIEETLIQKINHRIDAIDVLELRIDQFENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVIISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            4531..5070
                     /locus_tag="VB241_002210"
     CDS             4531..5070
                     /locus_tag="VB241_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEP1245477.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(5220..5540)
                     /locus_tag="VB241_002211"
     CDS             complement(5220..5540)
                     /locus_tag="VB241_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1245478.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5684..6040
                     /locus_tag="VB241_002212"
     CDS             5684..6040
                     /locus_tag="VB241_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP1245479.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            6198..6578
                     /gene="gcvH"
                     /locus_tag="VB241_002213"
     CDS             6198..6578
                     /gene="gcvH"
                     /locus_tag="VB241_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEP1245480.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
BASE COUNT         2439 a          948 c         1111 g         2254 t
ORIGIN      
        1 atgtgaaaag aatatttaca tactaaaaac ccttaaagtt gtacaattcg ttaatatttt
       61 tagaatcaga taacaaaaaa atgtgcaaaa tcacttttgc acatttacaa atactagttt
      121 tctgataaga ttaataataa cactttaaat agcgtttaat aaatgaaggg ggcaagtctt
      181 atgacgtttt acaatttcat catgggtttt caaaatgata acacaccatt tggtatattg
      241 gccgaacacg ttagtgaaga taaagcattc cctcgattag aagaaagaca ccaagtaatt
      301 agagcatatg tgatgtctaa ttacacagat catcaattaa ttgaaactac aaatagagct
      361 attagcttat atatggcaaa ttaatttgag tagtaccaat tatgatgtat tagtgcatcc
      421 caaatatctt ttgttttaaa gtttatttca tcatttctta tcgaaaatgg tgtaataatg
      481 tctttatcta accaagtgtt tataagttca tttggtacac catctaacaa catttcactt
      541 ttactaatta taaaacattc ccagtcaagt gaaacatttt gtggattcac ataattacat
      601 tgattatgat tatccataaa cactcactcc tttaaaattc tgtactcttc attgcgtttt
      661 accccgtcac attatctttt aaactaaaat catcattact tatgaaaaaa atgttcatca
      721 aaagcaaagg ttttcgctac cgaaaaagtt taaataatgg tttaatatat ttggtactca
      781 ttttaataaa aagagaatac attttgagct atcaatactt tttattgaag aggtgttatt
      841 gttggctaaa acgttatatt taatgcgcca cggacaaact ttgtttaatt ttaagggact
      901 aattcaggga tttggagatt cgccgctaac agaacttgga attgctcaag ctcaaaaggc
      961 acgtagctat tatgaaacta aggggataaa cttcgattta tatgcatcat caacgcaaga
     1021 acgtgcaagt gacacacttg aaaatgttgc acctaatcaa tcgtatcaac gttttaaggg
     1081 actgaaggaa tggcattttg gattatttga aggtgagtca gtctatctat ttgataatct
     1141 atacaagcct gaagacttat tcggagatcg aattgttcct ttcaaaggag aggcaaggca
     1201 acaagttgaa gatcgcattg tgaaaacttt acatgacatt atgtctcaaa caaagaataa
     1261 tgcattagtc gtgagtcatg gaacaataat gggagtattt ttaagatatt gccttaaact
     1321 agatgaagca ttaaagcata atatcggtaa ttgtaatatc ttgaaatttg aatatgacaa
     1381 tggaacattt aaatttgttg agttaattga tccaaattta taataaataa agcgttatac
     1441 atagacacaa gtatgtcttt tgtataacgc ttttaaaata caactacatg acgctttaga
     1501 ataataattg aataagtttt tgtaatatca tcagagcaac aataataatt attatgcttg
     1561 atattttatt gattattgtt aataattttc cggttttatc aattgatcca accatttttc
     1621 ctaaaattgc gagtaaaaag aaccataacc atgaaacgct aatacaagcg attgtaaacg
     1681 caattttatt gctgccacta tataatgcag cactactacc aattacacca attgtatcta
     1741 aaatagcatg tggattgagt aatgaaactg ataaagcaaa acttacttgt ttcattggag
     1801 acataatttg agcttctcca tctgttgagg gtttatcatg ccaaatggtc caagccatat
     1861 acatcaagaa aattaaacca actatataaa taattgcttg aagtataggt aaagacataa
     1921 taatgataga aactcctacc actgcaataa taataaataa gctgtctgac aacccggctg
     1981 taattattgc aggcaataca tatctatatt ttggttgatt agctccttgg ttaaaaataa
     2041 atacattttg tgcacctaaa ggtaaaatta gaccaatcgc taagataaat ccatgaataa
     2101 ttgcggttac cattaagttt tgacacccct aaaacataga atattctgaa aaaacttatt
     2161 gatatcatac caaataaaaa acaactactc aatagtagag tagttgtatg aaaaatagat
     2221 taatatatga tttgaattta tgagaaatgt agaatatata aaactgtatt acttattaat
     2281 caaatcataa aaaagccaat gttcatcgaa ataggtattt ccgattttaa ttgcattttt
     2341 ctcaaaacca agaatgtcga atcctagtgc actatagaaa actttagcac ctatgttgtt
     2401 tgacacaatt gatgtaagta aagattcgta attgttctgc cgtgcataat taataatgaa
     2461 attaatgagc tcgctattaa tcgatttatc gttattagta acaaagttgt atttaattaa
     2521 tgatttatgt tcttttccaa cgtatcgaat ttgttctaag gcagctgttg cgataagtgt
     2581 ttcgttttca taacagccaa atacaataca tttcggtgaa ccttgattta aaatgtcatg
     2641 tattaaggtg tcagtcattg tttgactata atgtgaatct tgagtgaatt cttcattgac
     2701 tgtcgaaaca agtctcttat actcattaaa atcattattc gttaatgttt taatttcaat
     2761 cattactgac ctccttaatc attattaaga aagattttgt actaaaaaaa tttcttaatt
     2821 acatattaaa atgtttatta aaattttgca atcattgatt tatatgcaca ttcttgtaaa
     2881 tggattatca atttaattat atttattttt aagttcaggg aattatggaa ttggttattg
     2941 gtcgacactg gacttttatt tgtagacggt atgcgaagaa gtcaagccag tattggaacc
     3001 ggtcacattt atgatgacta agggacaatc attttcagtt aaatattgcc agaaatttca
     3061 atcacgatta gaaaaaataa ccaaccaaaa atttttggtt ggcaaatttg aattttatgt
     3121 gaattttatt aataacgttg aataatctct ttaagttaat gctaatctac aacatttaca
     3181 tttaaatcga cattaatatt tccttgagtc gcttttgaat atggacaaaa ttcatgagcc
     3241 atttgtaaat atttttcagc ttcttcttga gatataacat ttttaattgt cgcatcaatt
     3301 gaaacactta atttaggact ttctgagtct ggatcatctt ctagtctcac tgttagtgtt
     3361 acttctggat gagcatcacg cactttgttt tgctttaaaa ttaggtcgaa ggcaccgttg
     3421 aagcaagatg cataacctgc tgcaaataat tgttcggggt tagtagcttt accatctgct
     3481 tgagcaggcg gaacgatatc aatatctaat gctcgatcat cagtataaac atgtccttta
     3541 cgtccgccaa cattcgtcgc tttagtttca taatgtattg ccattcttat aacctcctaa
     3601 tttaaaatac atttatgctt tacccattcg aaaatacctt aatcatattc atttatagca
     3661 ttgtttgatt gaaggataaa aagttgttgt ttacaataaa aataatgagt atctgaaatg
     3721 agggatttac tatgacacat gtggaagtag tagcgactat cacgccacaa ttatatatcg
     3781 aagaaacttt aattcaaaaa attaatcatc gtattgatgc aatagacgta ttagaattac
     3841 gaattgatca atttgaaaat gtcacagttg atcaagtggc agaaatgatt acaaagctga
     3901 aggttatgca agattcattc aaattattgg ttacgtatcg tacaaagtta caaggtggct
     3961 atgggcaatt tacaaatgac tcgtatctta atttaatatc agacttagca aatatcaatg
     4021 gcatagatat gattgatata gaatggcaag cagatattga cattgaaaaa catcaacgaa
     4081 tcattacaca tttgcaacag tataataaag aggtgattat atcacatcat aatttcgaaa
     4141 gtacgcctcc attagatgaa ttgcaattta tattttttaa aatgcaaaaa ttcaacccag
     4201 aatacgttaa attagcagta atgccacata ataaaaatga tgtgctaaat ttattgcagg
     4261 caatgtctac attttcagat actatggact gcaaagttgt tggtatttca atgtctaaac
     4321 ttggactaat aagtagaacg gctcaaggcg tttttggtgg tgcgttgact tatggttgta
     4381 tcggagaacc acaagctcca ggacagattg atgttactga tttaaaagca caagtgactt
     4441 tatactaata aaatgaatta ggttgctttt gtgtaagtgc tgtcttataa ttgagaagta
     4501 ttatcattgt tcgtggagga gcgattttag atggaattac aacaagcaat agctaataga
     4561 agaagtgtga aaaaatttaa aagagatatg cacatagatg acgcattgct atatcaagca
     4621 attgagaaag ctgctgatgc tccaaatcac ggaatgaggg aaccatggag agttgtgcat
     4681 gttccgaaag acagattagg agatatgagt aaggatattt ctaaatttgc atttcctaat
     4741 gaattagata agcaacaatg tcattatgat gcagtaacga aactaggtgg catgttattg
     4801 cttattttaa aaacagatcc aagacaacgt caaaatgatg aaaactactt tgcatttggt
     4861 gcatatgcac aaaatcttat gttgttactt tatgaagcgg gaataggtac atgttggaaa
     4921 tcgccattat atatctatga tcctaaagta agaaaaacac ttggtataaa gaaagatgaa
     4981 gttcttgctg gattcttata tttaacggat ttagaagaag atatgcctaa agcaccacgt
     5041 aaaaatagaa acttaattac attatattaa tatgtataat tatagaaaca ttaataaaag
     5101 ctgagtcatg aattgatgga catctatcga gttagagatt taatctaact tactagagtc
     5161 ggtacaatca cagtctcagc tttttattgt gcagtatata cacatttgta ttttagtatt
     5221 tatttaaaag tttctgctaa aaatgattca acttgttcag gtgacttagc atttgctgaa
     5281 tgaaggtgtg caattttgtc gccgttttta aatactagca agctagggat acccataact
     5341 tcattttcaa caactacatc ttctaattca tcacgattaa cagtatacca ttggtaatca
     5401 ttatattgtt ctacgattgg gtcaatccat aaatccatag cacgacagtc tgggcaccat
     5461 cctgcctcaa atttaacgat tacaggtgta tcgctattaa ttacagattt aaatgattca
     5521 ttacttttga ttgcttgcat tgtaacaact cctctagata gtttaataat ttttattata
     5581 gctaaattta tatcataata aaaaatttta gcttcaaaat gaaagccttt tgctatggaa
     5641 aatgatatat ttattttaaa tacataaagg aggttgcagt cgtatgatta aattttacca
     5701 atataagaat tgtacaactt gtaaaaaggc agcaaagttt ttagatgaat atggcgtaag
     5761 ttatgaacca attgatatcg ttcaacatac acctacaata aatgaattta aaacaataat
     5821 tgcaaataca ggcgtagaaa ttaataaatt gtttaataca cacggtgcga aatatcgtga
     5881 gcttgatttg aaaaataaat tacaaacttt atcagatgat gaaaagttag agttgttatc
     5941 atctgatggt atgttagtaa agcgtcctct agcagtaatg ggcgataaga taacattagg
     6001 atttaaagaa gatcaatata aagagacttg gttagcgtaa gtataatgta aacgtttact
     6061 aaatatctcg atatttagat tcattacatg taaaatgaaa taagctatac aattgttaat
     6121 ttttataaat atagttgaat agcatctagc cttatggcat cattaatgat gtaaagatta
     6181 attaggaggg gattctcttg gcagtaccaa atgaattgaa atattcaaaa gagcatgaat
     6241 gggttaaagt tgaaggtaat gtagcaacaa ttggaatcac agaatacgca caaagcgagt
     6301 taggtgatat tgtttttgtt gaattaccag aaacagatga tgaaattaat gaaggggata
     6361 cgtttggtag cgtagaatca gttaaaactg tatcagaatt atatgcacca atctctggta
     6421 aagtagttga agtcaacgaa gaactagaag atagtcccga atttgtaaat gaatctccat
     6481 acgaaaaagc atggatggta aaagtagaaa ttagtgatga aagtcagctt gaagctttat
     6541 taacagctga aaaatattca gaaatgattg gtgaataatc accgtgtaac tccttaatct
     6601 aagattgagg agtttatttt tagtctgagc aagtaaataa tatgaaagaa aatttattag
     6661 gtactattat ttggagtatt gctacatttt attattcaag aatgatgaaa ataatgaatt
     6721 tagctatttt aaaaataaaa attgggggag ta
//
LOCUS       DATTQF010000130         6688 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.130, whole genome shotgun sequence.
ACCESSION   DATTQF010000130 DATTQF010000000
VERSION     DATTQF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.130"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            69..2111
                     /gene="lip1"
                     /locus_tag="VB241_002214"
     CDS             69..2111
                     /gene="lip1"
                     /locus_tag="VB241_002214"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HEP1245481.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPRDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDLNANNNLPSQSRTKVSPSLNKSDQTSQREIVNETEIEKVQPQQKNQANDKITDH
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKGDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGSREEIEYQKKHSGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
     gene            complement(2503..3555)
                     /gene="icaC"
                     /locus_tag="VB241_002215"
     CDS             complement(2503..3555)
                     /gene="icaC"
                     /locus_tag="VB241_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HEP1245482.1"
                     /translation="MKKIRLELVYLRAIICAIIIVTHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDTSFQKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(3542..4414)
                     /gene="icaB"
                     /locus_tag="VB241_002216"
     CDS             complement(3542..4414)
                     /gene="icaB"
                     /locus_tag="VB241_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HEP1245483.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDPPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDMHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(4411..4716)
                     /gene="icaD"
                     /locus_tag="VB241_002217"
     CDS             complement(4411..4716)
                     /gene="icaD"
                     /locus_tag="VB241_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HEP1245484.1"
                     /translation="MVKPRQREYPTLKSSLNIIRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQKGRES"
     gene            complement(4680..5918)
                     /gene="pgaC"
                     /locus_tag="VB241_002218"
     CDS             complement(4680..5918)
                     /gene="pgaC"
                     /locus_tag="VB241_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HEP1245485.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            6082..6642
                     /gene="icaR"
                     /locus_tag="VB241_002219"
     CDS             6082..6642
                     /gene="icaR"
                     /locus_tag="VB241_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HEP1245486.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
BASE COUNT         2486 a         1016 c         1086 g         2100 t
ORIGIN      
        1 ttgagtttat tcctcgaata attatcaatt tgtttatcta aaataaaaaa atagaggtgc
       61 tgacaatgat gaaaagtcaa aataagtata gtattcgtaa atttagtgta ggtgcatctt
      121 ccattttaat agctacatta ctatttttaa gtggtggaca agcacaagca gctgagaagc
      181 aagtgaatat gggaaattca caggaggata cagttacagc acaatctatt ggggatcaac
      241 aaactaggga aaatgctaat tatcaacgtg aaaacggtgt tgacgaacag caacatactg
      301 aaaatttaac taagaacttg cataatgata aaacaatatc agaagaaaat catcgtaaaa
      361 cagatgattt gaataaagat caactaaagg atgataaaaa atcatcgctt aataataaaa
      421 atattcaacg tgatacaaca aaaaataaca atgctaatcc tagggatgta aatcaagggt
      481 tagaacaggc tattaatgat ggcaaacaaa gtaaagtggc gtcacagcaa cagtcaaaag
      541 aggcagataa tagtcaagac ttaaacgcta ataacaatct accttcacaa agtcgaacaa
      601 aggtatcacc atcattaaat aagtcagatc aaacaagtca acgagaaatt gttaatgaga
      661 cagaaataga gaaagtacaa ccgcaacaaa agaatcaagc gaatgataaa attactgacc
      721 acaattttaa caatgaacaa gaagtgaaac ctcaaaaaga cgaaaaaaca ctatcagttt
      781 cagatttaaa aaacaatcaa aaatcaccag ttgaaccaac aaaggacaat gacaagaaaa
      841 atggattaaa tttattaaaa agtagtgcag tagcaacgtt accaaacaaa gggacaaagg
      901 aacttactgc aaaagcgaaa ggtgatcaaa cgaataaagt tgccaaacaa gggcagtata
      961 aaaatcaaga tcctatcgtt ttagtgcatg gtttcaatgg gtttacagat gatattaatc
     1021 cttcagtgtt agctcattat tggggcggta ataaaatgaa cattcgccaa gatttagaag
     1081 aaaatggtta caaagcttat gaagcaagta taagtgcttt tggaagtaac tatgaccgcg
     1141 cagttgaact ttattattat atcaaaggcg gtcgtgtaga ttatggtgca gcacatgcag
     1201 caaaatatgg acatgaacgt tatggaaaaa catacgaagg aatttacaaa gactggaaac
     1261 caggacagaa ggtacacctt gttggacata gtatgggtgg tcaaacgata cgtcaactag
     1321 aagaattact gcgtaatggt agtcgtgaag aaatagagta tcaaaagaaa catagtggcg
     1381 aaatttctcc actattcaaa ggtaataatg acaatatgat ttcatcaatt actactttag
     1441 gaacgccaca taatggaacg catgcttcag atttagctgg taatgaagct ttagtgagac
     1501 aaattgtatt tgatatcggt aaaatgtttg gtaataaaaa ttcaagagta gacttcgggt
     1561 tggctcaatg gggtctaaaa cagaagccaa atgaatcata tattgattat gtcaaacgcg
     1621 ttaaacaatc taatttatgg aaatcaaaag ataatggatt ttacgatctg acgcgtgagg
     1681 gtgcaacaga tttaaatcgt aaaacgtcgt tgaaccctaa cattgtgtat aaaacataca
     1741 ctggtgaagc aacgcacaaa gcattaaata gcgatagaca aaaagcagac ttaaatatgt
     1801 ttttcccatt tgtgattact ggtaacttaa tcggtaaagc tactgaaaaa gaatggcgag
     1861 aaaacgatgg tttagtatcc gttatttctt ctcaacatcc atttaatcaa gcttatacaa
     1921 atgcgacgga taaaattcaa aaaggcattt ggcaagtaac gcctacaaaa catgattggg
     1981 atcatgttga ttttgtcgga caagatagtt ctgatacagt gcgcacaaga gaagaattac
     2041 aagatttttg gcatcattta gcagacgatt tagtgaaaac tgaaaaggtg actgatacta
     2101 agcaagcata atttataaag taaagggagg aattaataat gactgcagac ttctttcaat
     2161 taatcggatc attatttaga attctaaaag agttattcaa gtaatacctt ggcgggaccc
     2221 caacaaagag aaattggatt cccaaattct acaggcaatg caagttggcg gggccccaac
     2281 aaagagaaat tggattccca atttctacag acaatgcaag ttggggtggg acgacgaaat
     2341 aaattttgcg aaaatatcat ttctgtccca ctcccattgg catttacgta gtttaaatgt
     2401 gcaattagaa tatatgtata acaatattaa acacgcggta aaacgaagtc agtcaattca
     2461 aactgatttc gcccaccgcg tgtttttaac atagcttaat aattaataag cattaatgtt
     2521 caatttatat ggttgttttc caataataaa cctaaagata tagaattcac gcaatatcat
     2581 accgacacct atacataatc ctaaaatgaa tagtagtgat atcgctagaa agaccattgt
     2641 attatcctca aatatatttg tatatgcaaa caatgagtct agaatgattg gatgtaataa
     2701 ataaataaag aatgagaaag cactaatcat ttgaatcgta ttaaataaca ttgttttaaa
     2761 atgcgtgcaa atacccaaga taacaataaa cataatacta ttatatggtg ttaatgaata
     2821 tgaaaagctg gtaacgttcc aatagtctcc atttgctaac gcaataaaca caaaataagt
     2881 agctacagct aatacaatca taataactaa ataacgttct aagaaattta aaacacgttc
     2941 gtagttataa cccatatatg cacctaaaaa gaaataaaaa atccatccga atattatagt
     3001 gttttcactt aatggataat agtgtagcac ggtatcgtga aacgctgtgt tgttcgtaaa
     3061 gtaatataaa aatgattgct gtaaaataaa agataacaat aataatattt tactgttgaa
     3121 taggttatag ttaattttaa aaatgatata actcaaaata aagaattgca tgataacaac
     3181 gataaaatag ccataccatt gacctaatag gacattttca ataaattgtt tttgaaaact
     3241 tgtgtcagtt aataatgatt cactataact gtaaaacaaa cccattaata tgtaaggaat
     3301 caatatatat tttacgcgtg tagttaagta tctataggtg actttttggt aattcaaggt
     3361 tgtcagtaac tgtgacaaga taataaagca aggtgtacca aaaatgacaa tattacgaat
     3421 atagaactgt agtactaaag atcctccttc catattctca tgttttaaag taatttgtgt
     3481 taataaatgt gtaacaataa taattgcaca tataatggca cgtaaatata cgagctcaag
     3541 tctaatcttt ttcatggaat ccgtcccatc tcttaattaa atgctcaaaa gcatcatcac
     3601 taattaatat tctagggatg taataatcat tggagttcgg agtgactgct ttttcctcta
     3661 atgaaaaacc gtattttaac ccagcttttt tgattaccgg taatttatcg tcattcatca
     3721 agccataagg ataggctata gttttctgcg actttttaaa gtttttagtt agatattttt
     3781 cacttttgtt taaatctttt atgattgtag cttcagaagc tttcattaat tttgacttat
     3841 tatttttaga taagttatgc atatcgtggg tatgtgtttc aaattcccat aacccagttt
     3901 tatacatttc ttttagttct tttttactaa tcatatcgag gttgtgaaag ttttcttccc
     3961 caacatgacc tgtgataata aacccagttg ccggtatttt atattttttt aagattggat
     4021 aagcattttc ataaatagtt tcatccatat catcaaagtt aatccataca cttcgttttg
     4081 gaaacttacc ttttttcttg taatataaaa attctttcaa agttaaaaat ttagcatcat
     4141 gtgattttag ccattttatt tgagattcaa attgtgattg gctaacacta taatttttaa
     4201 tttctttact actagaaaag aagtaaatga aattattcag aaaattcgct tttcttacac
     4261 ggtgataatt taatgccaga gcactatttt ctttatattt cagtttttta ggtggatcgt
     4321 catctgcatt tgcaatatga tgaccatcca gtgtgcttac aggcaatatg atcaagatac
     4381 tcaacactaa aattattaat tttctatact tcacgattct cttcctttct gccatttttg
     4441 aatcaatatg ctaattgtaa aaaaaacaaa aatgataatc gcgaaaatgc ccatagtttc
     4501 aaatatatct aaaatttcag tattttcaat gtttaaagca acacgtattg tattgatact
     4561 ttcgtcatga atttcaaata tagtaccaat ataaacgagt agaacaacta aacaatatat
     4621 ccaaaagaca cacgatatag cgataagtgc tgtttctctt ataatgttta gcgatgattt
     4681 tagcgttggg tattccctct gtctgggctt gaccatgttg cgtaaccacc tttcttacgt
     4741 tttaatgctt ttggaaatgc gacaagaact actgctgcgt taataatcca gtatactgtc
     4801 ggataccaac ttacaaatat gagtccagcc atattctttt tctcgtagcg actatcaata
     4861 aagagtgcga ctgtaaattg aataacgttt ataaaagtca tagtaaatga tgatagtaga
     4921 aatattgaaa aactatatgt cataaatgta tagtctaaga agtttgctgt tatgaacaaa
     4981 tagcctaaat atagaagcac tatatatacc cataaaattg agatgatttg ctcaaacatc
     5041 aaaatatata aaggaaacct tttcgttttc attgtgctaa aaaagtctcg tagtaatact
     5101 tcgtgtcccc cttgagccca tctcacgcgt tgcttccaaa gacctcccaa tgtttctgga
     5161 accaacatcc aacacatggc aagcggttca tacttaatac gatatccacg taaatgcaat
     5221 ttccaagaaa ctgcaatatc ttcggtaatc atatcagtat cccagtagcc aacgtcgaca
     5281 actgcacttt ttttaaatag agtgaagaca cccgaaatag tattgactgc gccagcaagt
     5341 gtctgacttc gcttaataca gccaattaaa cttgcatatt ctatcgtttg aattttacct
     5401 aaaatagaac tcttatttcg aattctagga ttacctgtaa ctgcaccaag ttttggatca
     5461 tgtttgaaat tttcaatcat ataatatggt gcatcttgat caacgatagt atctgcatcc
     5521 aagcacatta cataatcata tgaagcctgt ttaatgcctt gattgagtgc attggcttta
     5581 cctctgtttt cttgtaaatc gacgaaaata aagtcattat tttctttaat tttatagatg
     5641 agttctgctg tattatctga acttccatca ttaatgataa taatttcttt tttctcgtat
     5701 ttgagtgcaa gaacattaga caacgtatct tcaatcgttt cactttcgtt ataacaggca
     5761 agtaaaaatg taatgccttc taattcatcc acatttatgt caggcttctt gttcaatgaa
     5821 tatctaattt ctctagtaaa atagaaataa attgaaccga caatccagta aatagacata
     5881 aatacaggat aaaaaagcaa aaagttaaaa aattgcaatt tctttaccta cctttcgtta
     5941 gttaggttgt aagccatatg gtaattgata gtattttaat ttgcaataga ttgttgttat
     6001 aattaaacgg aaatatttgt aattgcaact taattttcct gtaacatagt gtgattaatt
     6061 ttcagtaggg ggttttaaaa attgaaggat aagattattg ataacgcaat aaccttattt
     6121 tcagagaagg ggtatgacgg tacaacactt gatgatatag ctaaaagtgt aaatataaaa
     6181 aaagcgagtt tatattacca ttttgactcg aaaaaaagta tttacgaaca aagtgttaaa
     6241 tgttgttttg attaccttaa taatattatt atgatgaatc aaaataaatc gaactattca
     6301 attgatgctt tatatcaatt cttatttgag tttattttcg acatcgaaga aaggtatatt
     6361 agaatgtatg ttcaattatc taatacgcct gaggaatttt ctggaaatat ttacggacaa
     6421 atacaagatt taaatcaatc attaagtaaa gagatagcaa aattctatga tgaatcaaaa
     6481 ataaagatga caaaagaaga ctttcagaat ttaatattgc tgtttcttga aagttggtat
     6541 ttgaaagcat ccttttcgca aaaatttgga gcagtggaag aaagtaaaag tcaattcaaa
     6601 gatgaagtgt attcgctact aaatatattt ttgaagaaat aatttttgtt actagtttgt
     6661 aataattaac ttacttttgt aaaaaaag
//
LOCUS       DATTQF010000131         6682 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.131, whole genome shotgun sequence.
ACCESSION   DATTQF010000131 DATTQF010000000
VERSION     DATTQF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.131"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            113..1204
                     /locus_tag="VB241_002220"
     CDS             113..1204
                     /locus_tag="VB241_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HEP1245487.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
     gene            1558..1671
                     /locus_tag="VB241_002221"
     CDS             1558..1671
                     /locus_tag="VB241_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245488.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISGFSLNFSKQKYVTVN"
     gene            1744..2733
                     /gene="ccpA"
                     /locus_tag="VB241_002222"
     CDS             1744..2733
                     /gene="ccpA"
                     /locus_tag="VB241_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP1245489.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGKLIEKGAKSFALVGGEHSKKAQEDVLAGLTEVLNKNSLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(2831..4000)
                     /locus_tag="VB241_002223"
     CDS             complement(2831..4000)
                     /locus_tag="VB241_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEP1245490.1"
                     /translation="MQQHLSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAMKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKALNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDSSFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADTYTNGKIIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(4025..4657)
                     /locus_tag="VB241_002224"
     CDS             complement(4025..4657)
                     /locus_tag="VB241_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1245491.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAYLTAFRD
                     AEDQFEALLEITTLPEGRIYVARQDKLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSNPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            4826..6532
                     /gene="acsA"
                     /locus_tag="VB241_002225"
     CDS             4826..6532
                     /gene="acsA"
                     /locus_tag="VB241_002225"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEP1245492.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEL
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
BASE COUNT         2340 a          968 c         1250 g         2124 t
ORIGIN      
        1 tgtcaattta gggttgaact atacatatga tattgttaga atatttttta acattatatt
       61 ttattgcttt aaagtggaat atacttgaaa taattcgtag aggtgagtaa ggatgagtaa
      121 taaattagaa tcatacagaa gtgagattgt ctcactgaat catcaaattt tagacttatt
      181 atctaaacgt ggtgaactag cacaaaaaat tggggaagaa aaattaaaac aaggtacacg
      241 tatctatgat ccacaacgcg aaaaagaaat gcttaacgac ttaatcgata gtaacaaagg
      301 accattcaac gataatacta ttaagcaatt atttaaagaa attttcaaag cctctacaga
      361 tttacaaaaa tctgaaaatg aaaaacattt atatgtatca cgtaagttga aacctgaaga
      421 tacgattgta acatttgata atgggggcat tattggagac ggcaataaat catttgtatt
      481 tgggccatgt tcagttgaat catttgaaca agttgaagct gttgctaaaa acttacatgc
      541 taaaggtgaa aaatttattc gtggcggtgc atttaagcca cgtacatcac catatgactt
      601 ccaaggttta ggtgtcgaag gtcttaaaat acttaaacaa attaaagata aatatgattt
      661 aaatgttgtc agcgaaatcg taaatccaaa tgattttgaa gtggctgatg agtatttaga
      721 cgtattccaa attggtgcac gtaatatgca aaacttcgag ttattaaaag aagctggccg
      781 tacgaaaaag cctattctat taaaacgtgg tttatctgct acaatcgaag agtttgttta
      841 tgcagctgaa tacattgctt cacaaggtaa tcaaaacatt attttatgtg aacgtggaat
      901 ccgaacttat gaaaaggcga cacgtaacac tttagatatt tcagcagtac caattttaaa
      961 acaaggtaca cacttaccag tcatggtaga tgttacgcat agtacaggtc gtaaagatat
     1021 catgttacca actgcgaaag cagcattagc agtaggtgct gatggcgtta tggctgaggt
     1081 gcatccagat ccatctgttg cacttagtga tgcgggtcaa caaatggatt tagatgaatt
     1141 ccaagcattt tatgatgaat taaagccttt gtctgattta tataacgcta aaaagttaaa
     1201 ataatattcc aaggaaacta ttgactactt aactaataag tcatgttgaa gtagaatatt
     1261 atctttgaat cgacaatttt aaacttgtag ccattctaag agtatattac ttttagagtg
     1321 gctattattt tttgtataga aataaaggta tactgcactt aacgattgtt ataatacttc
     1381 gacacttgtt caatttcaca attattaaag attatgactg atagtagtaa ttaaaattat
     1441 aactatgaat tatctgtaaa atataataga ttcacatatt tgttactgaa atgtgaacat
     1501 ttttcaacaa atgcaattga tatttgaaaa ggctttctca aaatattaca attaaaaatg
     1561 aaaaaagttt atataaaatt aaaatatatc gttcgttatc atttagcgtt tgtttttatt
     1621 tcaggctttt cgctaaattt ttccaaacaa aaatatgtta ctgtaaatta aaatatggta
     1681 aactatgaaa atgaaatgaa aacatgttat tataatgaat aaaacgttta caaggaggaa
     1741 attatgacag ttactatata tgatgtagca agagaagcgc gtgtctctat ggccacagtg
     1801 tcgcgtgttg ttaatgggaa ccaaaatgtt aaagcagaaa ctaaaaataa agttaacgaa
     1861 gtcattaagc gtttgaatta tcgtccaaat gctgttgcta gaggtttagc tagtaaaaag
     1921 acaacaacag taggtgtgat cattccagat atatctaata tctattattc acaacttgct
     1981 cgtggacttg aagatattgc aacaatgtat aaatatcact caattatttc aaattcagat
     2041 aacgatcctg aaaaagaaaa agaaattttt aataacttat taagtaaaca agttgatggt
     2101 attattttcc ttggtggtac aattactgaa gaaatgaaag aattgataaa tcaatcatct
     2161 gtacctgtag tagtatcagg aacaaatggt aaggatgcac atatagcatc tgttaatatt
     2221 gattttactg aagctgcgaa agaaattacg ggaaaattaa ttgaaaaagg cgctaaatca
     2281 tttgctttag taggtggaga acattctaaa aaagctcaag aagatgtatt agcaggttta
     2341 actgaagtgt taaataaaaa tagtcttcaa ttaggtgata cattgaattg ttctggtgct
     2401 gaaagttata aagaaggcgt aaaagctttt gctaaaatga aaggtaacct tccagatgct
     2461 atattatgta tcagtgacga agaagctatt ggtatcatgc atagtgcaat ggatgctggt
     2521 attaaagttc cagaggaatt acaaattatt agtttcaata atacacgctt agttgagatg
     2581 gttagaccac aactttctag tgttattcag ccattatatg atatcggtgc agtagggatg
     2641 cgcttattaa caaaatatat gaacgatgaa aagatagaag aacccaatgt agttttacca
     2701 cacagaattg aataccgagg aactacaaaa taaattcaca aaattaggca ttcatctaac
     2761 gactcgactt atacgggtgt tggaagaatg ccttttattc atcatttaaa atcgttgcag
     2821 attaggtgac ttattgacga gtagattcgt accaactcgc tatatgcaaa gctaattttt
     2881 tatttttctc actaatttct tttgtgcggg ggacataggt ataatcattt aaacgatctt
     2941 cccatctttt aggtaataat tcagatgaat aatgtttcca tttattaatc cattctaacg
     3001 gtaaataacc actttgaatt ggttgatcaa ttaaacttaa gaatacatga ctccatgcac
     3061 gtggtacaac tctccaaata ttgtagcctc cgccaccaaa cataattatc tttccattcg
     3121 tataagtatc agctaaatat tttacaaaat atggaatttc atataatgaa tgtaacgtac
     3181 aatttagatg agttagtgga tcacgataat gtatatcgac accatttacg cttagaataa
     3241 tatcaggttt aaaactcttt acgactggct caactgttaa tttaaaacac tccaaaaatg
     3301 atgaatcttc ggtatacggt tcaagtggga catttacagt gtgtccatag ccgatatctt
     3361 caccgcgctc agtatagtga ccagagcctg ggaaaagaaa ctttccggtt tcatggatag
     3421 aataagtagt aacatggtta tcagcataga aactccattg tgtaccatcc ccatgatgtg
     3481 catcggtatc tatgattaaa acgcgttgat tgtattcttt agctaagtat tgtgcagtaa
     3541 ttgcaatatc attgtatata caaaaaccac ttgctcgacc aggttgagcg tgatgcaaac
     3601 caccacctaa gtgacaacca tttaatgctt tgcctgacat aataagatct gctaaagtta
     3661 aagcgcctcc aacaattgtg gcactatggc ggtgcatatg cttaaattga ccattctctt
     3721 catcatttaa tccatatttc atagcctcat cttcactgat aatgccatgt gaagcatgct
     3781 taatagcttc gacgtaatca tatttatgaa ttaacattaa ttcgtcatct gttgcaattc
     3841 taggttgtac tatttgttct ggagacaata aatttgcatt caaaagtagc tctgttgtta
     3901 attttaaacg catttgattg aagggatgtt ggtcatgaaa tcgatattgt aataacttat
     3961 ctgaatatac atatgcagtt tttgataaat gttgttgcat ataatccctc cgatattcca
     4021 aaaattaaaa gaaaaaccga ttcatataac gaatatcatc aaacgcttgt tgctgttcta
     4081 atgtaatatt tttgccaatt cttgccatta aacaattagc tggattactt gttatttctg
     4141 gatcatctgt agcgaatatt tcaagtccac cagttgccat taaccgctgc attaattttt
     4201 tatagtcaaa tacatctaac tttgaatttt ttaaatccca atgccagtaa tattctgtag
     4261 ttataacgat ataatcctcg aattctggtg tagaaaggct aagttgtatt aatttttctg
     4321 caagttgtaa ttgcctaaaa ttgatgctga cttcaattgc ccccaattcg attaaatatg
     4381 gaaggttacc tgtagaccag cgctcaattt catcaggata gtggaaagtg acataaccca
     4441 caatgagttt atcttggcga gcaacataaa ttctaccttc tggtaatgtt gtaatttcta
     4501 acaaagcttc aaactgatct tcagcatctc taaatgcggt taaatacgca tcgaaagtaa
     4561 gcgctttcaa atcttcgtgt gttaaaggac cttcaataac aaattgcttg tcatgtatgt
     4621 aataatcttc ggattgatac gtctttaaat gattcatatt ttcaactcct caatcgacgt
     4681 cgtgattgta ttaaattcat tatatagaaa atttacaata attaatacta gaaaaaggat
     4741 aaaagtaaaa attttgaata attagaaatg ttatgtataa tattgagaaa gaaagcgttt
     4801 tcacataaca aagggggagt ttcaaatgaa agtcgaagtt tataaaggag cgcaaggcaa
     4861 acataacctt aaagattatg aagaaacata taatactttt gattggaaag acgtagaaca
     4921 agcattttct tggagtgaaa ctggaaaaat gaacatggca tatgaatgta tagatcgcca
     4981 tgtagatcaa ggattagggg ataaaatagc attaaattac aaagatgaac acagaaaaga
     5041 atcgtatact tataaagata tgcaacggtt atctaataaa gcggcgaatg ttttgtctga
     5101 acatgcagaa gttgacaaag gtgacagagt atttatattt atgtcgcgta cacctgaact
     5161 atattttgcg ttgttaggtg ttttaaaaat tggtgcaatt gttgggccgt tatttgaagc
     5221 atttatggaa aaggcggttg cggatagatt agagaacagt gaagctaaag tgttaattac
     5281 taataaggca ttgttacctc gagtacctgt agataaatta ccgaacttga aaaaaattgt
     5341 tgtcgtagat gaggatgtag aagacaatta catagacttc attagtttga tggaaactgc
     5401 tagcgatgaa tttgacattg aatggttaaa gtcggatgat ggtttgattt tacattatac
     5461 atcaggttct actgggcaac ctaaaggtgt attgcatgtt caacaagcga tgttagtgca
     5521 ctatatttct ggaaaatatg tattagattt acaagaagat gatgtttatt ggtgtacagc
     5581 agatccaggt tgggttacag gaacatctta tggtattttt gcaccatggt taaatggcgc
     5641 tacaaattgt atagctggtg gtcgcttttc accagaacaa tggtatagta tgattgaaga
     5701 ttttaaagtg acgatttggt atacggcacc aacagcttta agaatgttaa tgagtgctgg
     5761 tgacgatatt gttgagaaat atgacttgtc atcgttacgt tcgattctat cagtaggtga
     5821 gcctttaaat cctgaagtta taaaatgggc gaaaaaagta tacggtttaa cggtgttaga
     5881 tacttggtgg atgacagaaa caggtggaca tatgattgtt aactatccaa cgatggacgt
     5941 caagcttggc tcaatgggca aaccattacc tggtattcaa gctgcaatta tcgatgatgc
     6001 aggtaatgaa ttaccaccaa atcgaatggg caaccttgct ataaaaaaag gctggccatc
     6061 aatgatgtat cgtatctgga agaatccaga aaaatataag tcatatttta ttggagactg
     6121 gtatgtatct ggtgattcgg catataaaga tgaagatggt tacttttggt tccaaggacg
     6181 tgttgatgat gtaattatga cagctggcga acgagttggg ccatttgagg ttgagtctaa
     6241 attggttgaa cacgaagcag ttgccgaagc aggaattatt ggtaaacctg atccggttcg
     6301 cggtgaaata attaaggcgt ttgttgcact gagaaaagga tatgaactaa cagacgaatt
     6361 aaaagaagaa attcgtttat ttgttaaaga aggtttgtcg gcgcatgcag caccacgtga
     6421 aatcgaattt aaagataaat tacctaaaac acgctcaggt aaaattatga gacgtgtatt
     6481 aaaagcttgg gaattaaatt tagatgcagg ggatttaagt acaatggaat aatgacatga
     6541 atgttataga agatttcttt cgaagaataa agggtgacaa catatttcat gttaatgttt
     6601 aaataatcgt ttactttacg ataagcaata taaagagctg ttaacttgtg tcatatcatt
     6661 tcgtagaaaa catttgaaaa tg
//
LOCUS       DATTQF010000132         6651 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.132, whole genome shotgun sequence.
ACCESSION   DATTQF010000132 DATTQF010000000
VERSION     DATTQF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.132"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..821
                     /locus_tag="VB241_002226"
     CDS             <1..821
                     /locus_tag="VB241_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1245493.1"
                     /translation="ENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHA
                     KETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKD
                     HVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTDTSPRYISIA
                     KSDMKHIMDVISTFAISYDGYIFKEAGSIINLHTINNALKSACRVNNIPIITSHALRH
                     THCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM"
     gene            872..1704
                     /gene="sph"
                     /locus_tag="VB241_002227"
                     /pseudo
     CDS             872..1704
                     /gene="sph"
                     /locus_tag="VB241_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(1961..2980)
                     /locus_tag="VB241_002228"
     CDS             complement(1961..2980)
                     /locus_tag="VB241_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin/hemolysin toxin family protein"
                     /protein_id="HEP1245494.1"
                     /translation="MIKQVCKNITICSLALSTALTVFPASSYAEIKSKITTVSEKNLD
                     GDTKMYTRTATTSDTEKKISQSLQFNFLTEPNYDKETVFIKAKGTIGSGLKILNPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNSTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRG
                     GLTGNITKEKNYSETISYQQPSYRTLIDQPTTNKGVAWKVEAHSINNMGHDHTRQLTN
                     DSDDRVKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKNDKGKSR
                     FIVHYKRSMDDFKLDWNKHGFWGYWSGENHVDQKEEKLSALYEVDWKTHDVKLIKTIN
                     DKEQK"
     gene            complement(3002..4057)
                     /locus_tag="VB241_002229"
     CDS             complement(3002..4057)
                     /locus_tag="VB241_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukocidin family pore-forming toxin"
                     /protein_id="HEP1245495.1"
                     /translation="MKNKKRVFIASSLSCVLLLLSAANTEANSANKDSQDQTKKEHVD
                     KAQQKEKRNVNDKDKNTPGPDDIGKNGKVTKRTVSEYDKETNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHRNETNASWLKYPSEYHVDFQVQRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSLGGKFDSTKGIGRTSSNSYSKSISYNQQNYDTIASGKNNNRHVHW
                     SVVANDLKYGNEIKNRNDEFLFYRNTRLSTVENPELSFASKYRYPALVRSGFNPEFLT
                     YISNEKSNEKTRFEVTYTRNQDILKNKPGIHYGQPILEQNKDGQRFIVVYEVDWKNKT
                     VKVVEKYSDQNKPYKEG"
     gene            4489..5712
                     /locus_tag="VB241_002230"
     CDS             4489..5712
                     /locus_tag="VB241_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEP1245496.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANFVAEIGSGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKDDKLYGRG
                     TTDMKGGLMALVIALIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LMIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYAE
                     LKKNDTKHELDVAPMFKSLIGKDISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINNVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVDKDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            6151..>6651
                     /locus_tag="VB241_002231"
     CDS             6151..>6651
                     /locus_tag="VB241_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP1245497.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNA"
BASE COUNT         2236 a         1053 c         1047 g         2315 t
ORIGIN      
        1 aagaaaatac ctaccgtcat tatagaaatg cattacaaca tatacaaaaa cataaaatag
       61 gtaaaatgga gttatcaaag ataaatagac aagtttatca gaaattcata aacgattatt
      121 caaaagaaca cgcaaaagaa actataagaa aaacaaacgg tgctattcgg tcagctttag
      181 atgacgcatt gtatgatggg cttattttta aaaatcccgc ttataaagtt aattataaag
      241 ccggaaaacc tacgaagtca gaacaagaaa aattcatctc ggtaactgaa tatgaaatac
      301 taaaagatca cgtcagaaag aagagaactc gttcatcatt agcgctattc ataatgattt
      361 gtacgggttg tcgtgtcagt ggtgcaagaa atataaagat tgagcatatc aaccaagtga
      421 aaaacactat atttattgac gagcgaaaaa ccgatacttc ccctagatat atcagtatcg
      481 ctaaatctga tatgaaacac attatggacg tcataagtac atttgcaatt agctatgatg
      541 gttacatttt caaagaagcc ggatctataa ttaaccttca tactatcaat aatgctttga
      601 aatcagcctg tagagtcaat aatataccaa ttattacatc gcacgcatta agacacactc
      661 attgttctta tttactagca aaaggtgtat ctatacatta catttctaaa agattaggtc
      721 ataaaaatat agcaataact acatccgtgt attctcattt gttagaagaa aaatttaatg
      781 aagaggacaa aaaaacaact aaaattttag aaagtatgta atttagggac ccattaggga
      841 ctccaaaccc aataaatact gttgttacaa ggtttctatg tatccaaact gggggggcaa
      901 tataaacgcg cttatttaat cggacaatct tcttatatta aaaataatga tgtcgtaata
      961 ttcaatgaag catttgataa tggtgcatca gacaaattat taagtaatgt gaaaaaagaa
     1021 tatccttacc aaacacctgt actcggtcgt tctcaatcag gttgggacaa aactgaaggt
     1081 agctactcat caactgttgc tgaagatggc ggcgtagcga ttgtaagtaa atatcctatt
     1141 aaagaaaaaa tccaacatgt attcaaaagc ggttgtggat tcgacaatga cagtaataaa
     1201 ggctttgttt atacaaagat agagaaaaat ggtaagaacg ttcacgttat cggtacacat
     1261 acacaatctg aagattcacg ttgtggtgct ggacatgatc gaaaaattag agctgaacaa
     1321 atgaaagaaa tcagtgactt tgttaaaaag aaaaatatcc ctaaagatga aacggtatat
     1381 ataggtggcg accttaatgt taataaagga actgaagagt tcaaagatat gcttaaaaac
     1441 ttgaatgtaa atgatgtttt atatgcaggt cataatagca catgggaccc tcaatcaaat
     1501 tcaattgcga aatataatta tcctaatggt aaaccagaac atttagacta tatatttaca
     1561 gataaagatc ataaacaacc aaaacaatta gtcaatgaag ttgttactga gaaaccgaaa
     1621 ccatgggatg tatatgcctt cccttactac tatgtataca atgatttctc agatcattat
     1681 ccaatcaaag cctatagtaa atagcactca acaaaaaact cgcttcgttc taaaaagtcg
     1741 aagcgagtta tattgttaaa atttgaattg actaacttta aactgatatc tcatcgtttt
     1801 catttcttta acttccacta tacctctaca tgcctcatat gcacctaaca atatcaagtt
     1861 aggttatcca taataaaaga tacagtgtct caagctatct atcccaaaac aaataaatag
     1921 ttacctacta cgtgatacgc agcaggcaac ttgcgaaaac ttatttctgt tctttatcat
     1981 taattgtttt aattaatttt acatcatgtg tcttccaatc aacttcatat aatgctgata
     2041 atttttcttc tttttgatct acatggtttt caccagacca atagccccag aaaccatgtt
     2101 tgttccaatc taatttaaag tcatccattg atcgtttata atgaacgata aatcttgatt
     2161 tacctttatc atttttatcg tgtgacatta cagctaaaaa ttctggatta aatccttctg
     2221 acacagttac aggcatttta tttttaggcg tgaaattatc tttcgcccat aggtttccgt
     2281 ttcgagttaa tgaaaagatt tcacttttca ctctatcatc actgtcatta gttagttgtc
     2341 tcgtatgatc atgtcccata ttattgatag aatgtgcttc tactttccaa gctacacctt
     2401 tatttgttgt aggttgatca ataagtgtgc ggtaagatgg ttgttgataa ctaattgtct
     2461 ctgaataatt tttctctttt gtaatatttc cagttaaacc accacgatta attgaaaaat
     2521 ctccacctgt tttatagcca tacgtatatt taacttctct tgattcatct tgatttttag
     2581 gcgcaaaatc agtcacattt gtactgttgt tgttatcatc aacgttttga atcgagactg
     2641 aataagaacc aggccatctt aatgtactat tccaataacc atttggattt aaaattttca
     2701 atccactgcc aattgtacct ttcgccttaa taaatactgt ctctttatca taatttggct
     2761 cagtaagaaa attaaattgt aagctttgtg agattttttt ctctgtatca ctcgttgtag
     2821 ccgtacgtgt atacatcttc gtatcaccat caaggttctt ctcggaaact gtagtaattt
     2881 tagatttaat ttctgcataa gaagatgctg gaaatacagt taaagctgtt gatagcgcta
     2941 aactacagat tgtaatattt ttacatactt gtttaatcat ttgtcatccg tcccttctac
     3001 tttatccttc tttatatggt ttattttgat cagagtattt ttcaacgact ttaactgttt
     3061 tatttttcca atctacttca taaactacaa taaatctttg accatcttta ttttgttcta
     3121 aaattggttg tccataatgt ataccaggtt tatttttcaa aatatcttga ttacgtgtat
     3181 atgtaacttc gaatcgtgtt ttttcatttg acttttcatt agaaatataa gttaagaatt
     3241 ctgggttaaa tccgcttctt acaagcgcag gatatctata ttttgatgca aaacttaatt
     3301 caggattttc tacagtagat aatctagtat ttctatagaa taagaattca tcatttctgt
     3361 tcttaatttc attcccgtat tttaaatcgt tggcaacaac agaccagtgt acgtgtctat
     3421 tattattttt accgctcgca attgtgtcgt aattttgttg gttataactg atagatttag
     3481 aatagctatt agatgatgtt cgtccaatac cttttgttga atcgaattta ccacctaagc
     3541 tataagaaaa tgtactatct actttcgcag tggaaatttt attttttggc agttgatcta
     3601 aaatttcagt tttagggttt ctttgtactt gaaaatcaac gtgatattca ctcgggtatt
     3661 ttaaccatga tgcgtttgtt tcatttcgat gagattcaaa ctttaaattt gaatgaattg
     3721 acccttgttt tttgacaagt aagacattct tgtcatatgt tggatcatcg ataaagtcga
     3781 attgtaaatt ttgtaaaata tttgtctctt tatcgtattc agatacagta cgctttgtaa
     3841 ctttaccgtt tttaccaata tcatcaggtc ctggtgtatt tttatcttta tcattcacat
     3901 tacgcttttc tttttgttga gccttatcaa catgttcttt cttagtttgg tcttgtgagt
     3961 ctttattcgc tgaattcgct tctgtatttg ctgctgataa caataaaagt acacatgata
     4021 atgatgacgc aatgaatacg cgctttttat ttttcattgt tataaccttc tttcttatat
     4081 ttaatattgt tgataggtat cgacatgtga ataatatcac aaaaactaag aatatatatt
     4141 taactattta ttaaatgatt ttgtttatat tattaaatac ttttttcgat ataaaattaa
     4201 tgtgtgtaca aagtgattaa attagtaaat cgcattattt agtagttaat caccatggat
     4261 ctatatgtta tacatgctta aactaaattt ttttcataca atccgactgg acgattcaca
     4321 atgaaaaaaa cacttactat cagtgattat tagactactg atacaaggtg catattttaa
     4381 gatgtaatca tgaacatagc acgtcacttt gttacttaaa attaatgttt aaatacaata
     4441 attatgttta tataaatata tatactactt tgaaaaggtg tgagcttaat gacaactttt
     4501 agtgaaaaag aaaaaattca attactagca gatattgttg aactacaaac tgaaaataat
     4561 aatgaaatag acgtttgtaa ttatttaaaa gatttattcg acaagtacga tattaaatct
     4621 gaaattttga aagttaatga acaccgcgcc aatttcgttg cagaaatcgg tagtggctct
     4681 cccatacttg cattgagcgg tcatatggat gttgttgatg caggaaatca agataattgg
     4741 acttatcccc cttttcaact cacagaaaaa gatgacaaat tatatggtcg aggcacaaca
     4801 gatatgaaag gcggtctgat ggctttggtc atcgcactca ttgaattaaa agaacaaaac
     4861 caattacctc aaggaaccat tagattactc gctactgctg gtgaagagaa agaacaagaa
     4921 ggcgccaaat tattagcaga taaaggctat ttagatgatg ttgatggtct aatgattgct
     4981 gaaccaactg gatctggaat ttattatgca cataaagggt ctatgtcatg taaagtaact
     5041 gcaactggta aagctgtcca tagctcagtt ccatttattg gtgacaatgc aattgataca
     5101 ctgcttgaat tttataatca atttaaagaa aaatatgcag agcttaaaaa aaatgatact
     5161 aaacatgagt tagatgttgc tcccatgttc aaatcattga taggaaaaga tatttctgaa
     5221 gaggatgcaa attatgcatc tggtcttaca gctgtatgtt cgattataaa tggcggtaaa
     5281 caatttaact ctgtaccaga tgaagcttca cttgaattta acgtaagacc agttcctgag
     5341 tatgataacg actttataga atcgtttttc caaaatatca ttaataatgt ggatagcaat
     5401 aagctttcac tcgatattcc aagcaaccat cgacctgtaa caagcgataa aaatagcaaa
     5461 ttaattacta cgattaaaga tgtagcttct agttatgtag acaaagacga tatatttgtt
     5521 tcggcgcttg taggtgcaac ggatgcttcc agcttcttag gagataataa ggacaatgtt
     5581 gatttagcca tttttggacc aggtaatcca ttaatggcac atcaaatcga tgaatatatt
     5641 gaaaaagata tgtatctgaa atatattgat attttcaaag aggcttccat tcaatattta
     5701 aaggagaaat aagaggtata ctgtcagctg ccctattcgc gtgctggcag ttttttattg
     5761 cgaattttaa gttgatagaa atagcgatga atattgtttg aatgtaaata ttcaaatatc
     5821 aacaagcata tttccattga ataggtgatg taaaactgaa actatagcgt tgatttgtca
     5881 tacttaaatt gcattgtctg ttgaaattgg gaatccaatt tctctttgtt ggggccccac
     5941 cccaacttac attgtttgta gaatttcttt tcgaaattct ctgtgttggg gccccgccaa
     6001 cttgcattgt tcgcagaatt tctttttgaa attctctgtg ttggggctcc tgactagaat
     6061 tgaaaagtaa tacacatcag taacatttct attttcattt atgatattat ctaattatta
     6121 atttataata taaaggagta tgcaattaaa atgaacaaag tccacaaacc tttatatttt
     6181 tatttaatgc ttttcttctc tacaactatc ataggtgcac ttttattgta tttacctttc
     6241 actggtaaaa agccaatatc ttttttagat gccctattta tagcttcaag tgcatttaca
     6301 gttactggct tgtccccagt tgatatagga tcacagttta atatacttgg tgagatagta
     6361 atactattat taatacaaat aggtggtcta ggtatcgtaa ccgtaaccct attgacacta
     6421 gtatttttaa atagaaagat atcaatgaaa aatagatttt tgattatggt tacatggaat
     6481 attgacgaac ctggtggtgt tattaagcta attaaacact tggctattta tagtttagtc
     6541 actgaattaa ttggtatgat ttgtttgtgt ttatctttta taccaaaatt tggtataggc
     6601 aaaggattat ttttaagctt attcacatca gtatcagctt ttaataatgc t
//
LOCUS       DATTQF010000133         6575 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.133, whole genome shotgun sequence.
ACCESSION   DATTQF010000133 DATTQF010000000
VERSION     DATTQF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.133"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1458
                     /locus_tag="VB241_002232"
     CDS             <1..1458
                     /locus_tag="VB241_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1245498.1"
                     /translation="NLTGIEKAIINRLNLFKEMGRPVQCVFLSWNRYLYRNAQNYITS
                     SDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAH
                     FQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTK
                     LEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTA
                     PIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKL
                     DVEKRINHTIPVVNIPVGYSETIDTPVQTLDQHSVKLISVARYSPEKQLHQQIELVKR
                     LVPYVPKVELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITS
                     NMEGFSLALLESLAHGVPVISYDIKYGPGELITSDFNGYLITKNDEDALFDKVKYVID
                     HPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1584..3056)
                     /locus_tag="VB241_002233"
     CDS             complement(1584..3056)
                     /locus_tag="VB241_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1245499.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDLFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGTGNDIKNFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKDKNHIISIARLVENKQITH
                     QIEVIKQLVTKHPKIQLNIYGHGNGLSEYRQLVEGYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPPGDINQMVE
                     KVDQLLNNSQMMQQFSINSIESAQQYNATTISTKWQKILN"
     gene            complement(3175..>6575)
                     /locus_tag="VB241_002234"
     CDS             complement(3175..>6575)
                     /locus_tag="VB241_002234"
                     /inference="COORDINATES: protein
                     motif:HMM:NF021909.1,HMM:TIGR01168.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEP1245500.1"
                     /translation="NKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQE
                     AKAAENTSTENAKQDEASASDNKEVVSETENNSTQKNDLTNPIKKETNTDSHQEAKEA
                     PTTSSTQQQQNNATTSTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNTNNDV
                     TTKPSTSEIQTTPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNN
                     PEKLKELVRNDSNTDRSTKSVATAPTSVAPKRVNAKIRFAVAQPAAVASNNVNDLITV
                     TKQMITEGIKDDGVIQAHDGEHIIYTSDFKIDNAVKAGDTMTVKYDKHTIPSDITDDF
                     TPVDITDPSGEVIAKGTFDLNTKTITYKFTDYVDRYENVNAKLELNSYIDKKEVPNET
                     NLNLTFATADKETSKNVKVEYQKPIVKDESNIQSIFSHLDTTKHEVEQTIYVNPLKLN
                     AKNTNVTIKSGGVADNGDYYTGDGSTIIDSNTEIKVYKVASGQQLPQSNKIYDYSQYE
                     DVTNSVTINKNYGTNMANINFGDIDSAYIVKVVSKYTPGAEDDLAVQQGVRMTTTNKY
                     NYSSYAGYTNTILSTTDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKPMA
                     NVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKENGTTDGEK
                     DSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVKVT
                     LKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKDSNGLT
                     TTGVIKDADNWTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTLQNEKG
                     EVIGTTKTDENGKYHFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGGEVDVTIT
                     DHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSATKDH
                     HNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1909 a         1117 c         1018 g         2531 t
ORIGIN      
        1 aatttaactg ggatagaaaa agcaattatt aatcgtttaa atttatttaa agaaatggga
       61 agaccagtac aatgtgtatt tctgtcatgg aatagatatt tatatagaaa tgcccaaaat
      121 tatattacta gctctgacta tatcaatatg tatgatttct tccaagaagc aacatattta
      181 gaacgcaatg aaccatttga ctggttgtca tattggactg atgaatgtca ttatacttta
      241 aaacatgttg aaaatagtca tgactttaga atatatgatc aagagcgttt cttaatgtat
      301 gcccattttc aagatcctaa atatcgtata ttagattatg tgaatcattt tgatagtcaa
      361 agacgaaaag taaaacgtga tttttatgat gtgcgtggct ttttaagttg tagtaggatt
      421 cttgttgata aacaacaaac attatgtgaa tttttctata atccagaagg tgacacaaaa
      481 ttagaaaagt atttttctta caaagatggc aaaccagaag tacaaaaaat tattgtttac
      541 tatgctaaca aacaatattt ctttaataat gaaacggaat taggcgcatt tttcattaaa
      601 caactatatc aacatgggga tttatttttc agtgaccgta atgtgtatac agcacctatt
      661 ttcaacttaa cacctgagtc aatcccagta gtagctgtgc ttcacagtac acacattaaa
      721 aatattgatg cattagattc atcgccattt aaaaatgtct ataaagcgat gtttgaaaat
      781 ttatctcgat atcgtgccat tatcgtttct actgagcagc aaaaattaga tgttgaaaaa
      841 cgtattaacc atactattcc agttgttaat attcctgtcg gctatagtga aacaattgat
      901 acaccagtcc aaacattaga tcagcacagt gttaaattaa tttccgttgc tcgttattca
      961 cctgaaaaac aattacatca acaaattgaa ttagttaaac gcttagtccc ttatgtaccg
     1021 aaagtagaac ttcatatgta tggttttggt tctgaaagta aaaaattaaa tgaattgata
     1081 caaaaatacg gattggaaaa tcatgtttat ttacgtggct ttttaagcaa tctagaccaa
     1141 gaatacagcg atgcttactt aagtttaata acgagtaaca tggaaggttt ctcgttagcg
     1201 ctacttgaat ctttagcaca tggtgttcca gttattagtt atgatattaa atatggtcct
     1261 ggtgaattaa ttacttcaga tttcaatggt tatttaatta ctaaaaatga tgaagatgcc
     1321 ctatttgata aagtaaaata tgtgattgat catcctgaag tacaacaacg actgtcaaaa
     1381 ggtagtttgg caaaagcgca acaatatagt aaagcgtcgt taattaaaca atgggaccaa
     1441 tttgttcgtc taatataaat gtactttacc tttattttta taaaaaaaaa gcattttcct
     1501 atgtcattta atcaaacttg tcagttctta tgaacgtgga ttgttaaata gatgatttta
     1561 ggaaaatgct tttttctttt gacttagttt aaaatttttt gccactttgt actgatagta
     1621 gttgcattgt actgttgtgc agattctatg ctattaattg aaaattgttg catcatttgg
     1681 ctattattta ataattggtc aaccttttca accatttgat tgatgtcacc tggaggtact
     1741 aaatagccat taaatccatc ttgaatcagt tctgatggcc cataatctac atcataactg
     1801 atcactggtg tacctactga aagcgactct aaaattgcta agccaaaacc ttccatttta
     1861 cttgtcgata acatcagttc tgctttagca atctcttcat taatatgcgt cttaaaacca
     1921 tgaaatttaa catgttccga taaatgataa ccttctacaa gttgtcgata ttctgacaaa
     1981 ccatttccat gtccataaat attcaattga attttgggat gtttggttac taattgcttg
     2041 atcacttcaa tttgatgtgt aatttgtttg ttttcaacga ggcgagcaat tgatatgata
     2101 tgatttttat ccttttgatt gatgtcaaat tgatacttta agtttgccac gtagccaacc
     2161 ggaatattga taactggtat tttattttca atatattgtg aaatatcttg gcattgcttt
     2221 tctgttgata caacaatcgc tttataacgt gttaaattat taaacactgt tttataaaaa
     2281 ttttttatat cattaccggt gccggataaa tgtgtactgt ggagcacaac aataactgga
     2341 atactttgat ttaatcccgc aataacattt cctaattcat gaggacggtc taatatgatt
     2401 tgatcattat ttttacataa ttgatggaga aaatattgaa ctaattcatc ttctgtatca
     2461 aaaaattgtt gatgctggtc ttcatttaag ataacctttg tgagcgtgtt tttccctttt
     2521 atatcgtcga aatatttttg gatgacgatt tccccattag gtgtatagta attttcgagt
     2581 acaatccgtt gtccttcacc taaaattcga gaacaactta aaaagcctct tccatcatac
     2641 aattcacgtt ttacttttct tcttttatga tcaaaataat tcacatagtt taattgatga
     2701 tactgtttat ctaaaaaatg agcatacatt ataaattgct cttcatcata tattctgaca
     2761 tcatttgaat tttccacaaa tttcaacgtg tacctacatg acttttccca atactgtatc
     2821 cagttaactt gctttgtctt tttataattg attgcttttt gaaaatagtc atacattgta
     2881 aacagatcat tttcaatctg atgttgcttc gcatatgtgt atgaataagg attccattta
     2941 acatatacac attttgaaga tatgccgtgt tgtttgaaca acttcagtct atttatttgt
     3001 gctttttcta cacctgtaat tttactttct aaaattgttc ctaaaatgta attcatatta
     3061 tcgcctcata taagttttat tccgtatctt tattgtttat tttatatgaa aaatacatct
     3121 attgcatgtg taatcataaa aaaaccaggc cacaaggacc tgggtcaaat tgtattattt
     3181 gttttgtttt ttgcgacgac cgaataacaa taatgaacct aatgctgcga ataatccacc
     3241 aaataacgtt gcgttatttg agccgttatt ttcactacct gtttctggta atgctttggc
     3301 tttattgtga tggtctttag tagcactcat tggtttcaca ggtgtatgtt ttcctgcatc
     3361 tgaatctgag tcgctatccg aatctgagtc gctatctgaa tctgaatcgc tatctgagtc
     3421 tgagtcactg tctgaatctg agtcgctatc tgagtctgag tcactgtctg agtctgagtc
     3481 gctatctgaa tctgagtcac tgtctgagtc tgagtcgctg tctgagtctg aatcactgtc
     3541 tgaatctgag tcgctgtctg agtctgaatc actgtctgaa tctgaatcgc tgtctgaatc
     3601 tgagtcgcta tctgaatctg aatcgctgtc tgaatctgaa tcactgtctg agtctgagtc
     3661 actgtctgag tctgaatcac tgtctgagtc tgagtcgctg tctgagtctg agtcgctgtc
     3721 tgagtctgaa tcactgtctg aatctgaatc gctgtctgaa tctgagtctg agtcactgtc
     3781 tgatgtatct tcttcgaagt atccattatc aagtgtgaaa tcatcatgat ccgtaattgt
     3841 tacgtcaact tcgccaccat ctgcatcttt atcatcttca gttgtatttg tacctgtttg
     3901 tgttaagcca gcaggctttt caaaaataac tttgtattta ccgctatcta aattatcgaa
     3961 atgatattta ccattttcat ctgttttagt tgttccaatt acttcgcctt tttcgttttg
     4021 caatgtaact gtcacatctt taataccctt ttcagttgaa tcttgtttgc cgtctttatt
     4081 actgtcgtac caaacataat cacctaaact atattttggt gttttataga aaccactgtc
     4141 taatgtccaa ttatctgcat ctttaataac acctgttgtt gttaaaccat tagaatcttt
     4201 atcttcagct gtagtgtttt taaccgttgg cgtgtaacct gctggtgttt caaattctac
     4261 tgtatagtta ccattatcta aatctgtaaa tttatattta cccgaggcat cagtagtagt
     4321 tgtaccaata acttttccag tactatcttt taatgtaacc ttaacatctt tgataccagg
     4381 ttcattagca tcttggatac catctttatt cgtatcttcc catacatagt caccaagatt
     4441 atatttaggt tctttataaa aaccagtgtc taaagacata tcatctttac cattaatttt
     4501 aacagttaca gaactaccat ttgagtcttt ttcaccatca gttgttccat tttcttttgt
     4561 tggaagatat ccagctggcg tttcgaattt aacagtataa gtttctccgt ctttcaaacc
     4621 accgaattca taatgaccgt tagcatctgt tcttactgat tttgtagtac cgtccgggta
     4681 agttaatgta actaaaacgt ttgccattgg cttttctttt gaatctgtac cttgaacacc
     4741 gtctttatca acgtcttccc atacatagtc accaattttg tataactttt cttcaggttt
     4801 aacagtaccg tcaccaccac cagagtcagt agttgataaa atagtattag tgtaacctgc
     4861 atatgatgaa taattatatt tatttgttgt agtcattctt acaccctgtt gaactgctaa
     4921 gtcatcctca gcacccggtg tatatttact aacaacttta actatatatg cagaatcaat
     4981 gtcaccaaaa ttaatattag ccatgttagt accataattt ttattaatag ttactgaatt
     5041 ggtaacatct tcatattgac tataatcata aattttatta ctttgaggta attgttgacc
     5101 cgatgcaact ttatagactt taatttccgt attactatca attatagtac taccgtctcc
     5161 agtataataa tcaccattat ctgctacacc accacttttt atagtaacat ttgtgttttt
     5221 agcatttaat tttaatggat tcacataaat agtctgttct acctcatgct tagtagtatc
     5281 taaatggcta aatatagatt gaatatttga ctcatctttt acaataggtt tttgatattc
     5341 aactttaaca tttttactag tttctttatc tgctgtagca aatgttaaat ttaaatttgt
     5401 ttcatttggg acttctttct tatcaatata tgagtttagt tctaattttg cattgacatt
     5461 ctcatatcta tcaacataat cagtaaattt gtaagtaatt gttttcgtat ttaaatcaaa
     5521 tgttcccttc gctattactt cacctgatgg atctgtaata tcaactggtg tgaaatcatc
     5581 cgtaatatct gaaggaattg tatgcttatc atactttact gtcattgtat ccccagcttt
     5641 tacagcatta tcaattttaa aatcggatgt ataaataata tgttcaccgt catgtgcttg
     5701 aataacacca tcatctttaa taccttcagt aatcatctgt ttcgtcactg taattaaatc
     5761 attaacattg tttgaagcaa ctgctgctgg ttgtgcaact gcaaagcgta tttttgcgtt
     5821 tacacgtttt ggtgcaacac ttgttggagc tgtagcaact gatttagttg aacgatctgt
     5881 attgctatca tttctaacta attcttttaa tttctcagga ttatttttaa gttcttcttt
     5941 tgacacattt actggttctt ttggattagt tgcatctgta acttgattgt ctacttttga
     6001 aggcgttggt tgcggttgtg aattttcaat atttgtagat tcttgaggtg tagttggtgt
     6061 tgtttgaatt tcacttgtag atggttttgt agttacatcg ttatttgtat tatttggcgc
     6121 tttcttctct tctaaaataa cagatgtatc ttctgtcgca gttttatcag ttgaaggttt
     6181 aacattttct ttttcaatgt tttgaggctt agtttcagtt gaagttgtag cattattttg
     6241 ctgttgttga gtactagatg tagttggtgc ttctttagct tcttggtgtg aatcagtatt
     6301 tgtttctttc ttaattggat ttgttaaatc atttttttgt gtcgaattat tttcagtttc
     6361 tgacactact tctttattat cactagccga tgcctcatct tgcttagcat tttctgtact
     6421 agtgttttca gcagctttag cttcttggtt ccctagacca aaaatcaatg tcgtacctac
     6481 taaaatcgat gcagttccta cagtatactt tctaatcgag aatttgttta agcgattcga
     6541 aatcataccc ttttttgtta ttgccttttt gttat
//
LOCUS       DATTQF010000134         6557 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.134, whole genome shotgun sequence.
ACCESSION   DATTQF010000134 DATTQF010000000
VERSION     DATTQF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.134"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..520
                     /locus_tag="VB241_002235"
     CDS             <1..520
                     /locus_tag="VB241_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP1245501.1"
                     /translation="VKKKKKNDKDKDEYETKTEEKQRKVNQNILINIPIKSENNKYVV
                     VEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYSSSKSDDMAYLM
                     DNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDITKKDGK
                     YYVKNMTNSIGG"
     gene            525..785
                     /locus_tag="VB241_002236"
     CDS             525..785
                     /locus_tag="VB241_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245502.1"
                     /translation="MSGLFNFLVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            797..1180
                     /locus_tag="VB241_002237"
     CDS             797..1180
                     /locus_tag="VB241_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP1245503.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVVPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1215..3710
                     /locus_tag="VB241_002238"
     CDS             1215..3710
                     /locus_tag="VB241_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1245504.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            3764..5122
                     /locus_tag="VB241_002239"
     CDS             3764..5122
                     /locus_tag="VB241_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEP1245505.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFAGVLAFIFLVYRIYILFID
                     YKSVNKKLDIWTLFAYFKPHLLYLLIGTAIIFVICKLIAQLLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKTFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            5127..>6557
                     /locus_tag="VB241_002240"
     CDS             5127..>6557
                     /locus_tag="VB241_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245506.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLAITLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIDPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQYKPMPLF"
BASE COUNT         2304 a          681 c         1260 g         2312 t
ORIGIN      
        1 agtaaagaag aaaaagaaaa atgataaaga taaagatgag tatgaaacaa aaacagaaga
       61 aaagcaacgt aaagtaaatc aaaatattct aattaatata cctattaaga gtgagaataa
      121 taagtatgtt gttgttgaat atccttattt tactccaatt cctgatagtc aattaaataa
      181 agctaagatg gttgaggata acttgaaaga taataaacgt gaagataatc cgaaagcaaa
      241 agcatttatt gaagatttct ttaataaata ttcttctagt aagtctgatg atatggctta
      301 tttaatggat aatcctgagg gattagaagg aactagagaa gtttctcaaa taagagaaat
      361 tagattatat ccaaaaggcg atgattatgt tgctaaagtt gaaattttga tgaaagataa
      421 agattctcct ttggagaatt tggaacatta cacattagat ataacgaaaa aagatggtaa
      481 atattacgtt aaaaatatga ctaattctat tggaggttaa tgttatgagt ggtttattta
      541 attttttagt tttaggtgct gaccgtccat cacttggtgg agttggtgac tggataagta
      601 atgaagtggg tacgggtatt ggtttagtag tattggttgt tggtattgta aaatgggcta
      661 gtggtaaata tgggcatatg gttatattat ttgttgttgg tggcttttta ttcttagttt
      721 ctaaaggtcc tgaacaggta tttaatgcga ttgcaggtgt ttggaaaatg atttttggcg
      781 gttgaggtga ttccatttgg ataaaaaagc ttataattta aaacggactt ttgaacaacc
      841 gatagtgatg tatgaattta cagataaatt tcgtattaat aagggttttc gtttagattt
      901 ttgggctaca tttttgattg tatggtttat attatttttg ttattttggt atttattaca
      961 gcctgtcatt atgtctattg gtggacttat gtttatttac tttacggttg tcccttatta
     1021 tataacgaaa tatattgtta agttaaagca agatggtaag aaattatttt tcttcttatg
     1081 ggattttgta atatttgttt ttaatgttca gttaagaaaa gttaaattaa gttatgatga
     1141 agaagtagaa tatcatgata aaaaaataac atttaaatag cccctattgg ggttattttt
     1201 attggaggga tgacatgaat ttaaaagcac cgtatgcagg tttaagagat aatttgttat
     1261 taacgaaaac tggcgaagtt tgggcgtatt atcgtgttcg ttctgagtct atttcaacag
     1321 caaattatga tgcaaaagaa gaatcaaaaa agcgtatgcg ttatttttta gagtcgatta
     1381 aaggttatca agattttcat tttgaaatgt atgaccatga tttagatttg cgaaataaat
     1441 ttaaagaaat tagtaaagat tttgatgatg aagcttttaa tgtagctgaa tattattcaa
     1501 aagaaactat tgacgtatta gaacaagaat tacaaatgat tacgcataat tcttttgtta
     1561 tgggtgttaa aatacgtgaa ttagctgacg atgcagttac acttaaagag attttaaaaa
     1621 gtacattatc agatacggct gagcgtattg tttcgaattt tggttttgat gtgaatttag
     1681 acgataaaat tttagataag tataaaacat ttgaacgaga tttggctgat agttttctag
     1741 gtattggagg cgaacgttta actgagaatg agctggctta tattattcgg aagccattta
     1801 tatcaaatgc aacacatgac gtacaggaag aatcttcaag acgagctatt acagatattt
     1861 ataatgcagt aattgaccct actcgtttga gtgttttaga agttgaaaat gataatgaaa
     1921 aattttacac aacaacagtt gttgttgatg attttccgtt agatatggaa tatacacatt
     1981 tatttgagaa agctcaaaat atgccttttc cggttgaatg tcatgtaaaa gcaaaaatta
     2041 ttaataatga aaaagttaag aaaaaatttg accgtgcgaa aataaatcat aaacaacaag
     2101 caaaagaaaa gcaagatact ggcgatagtg cttcggaaga tgttcaagat aatttgtttg
     2161 tattgaatca aatggaacga gaagtgactg gctcaggtgt atttattgag tgggtattta
     2221 tgtttgtaat taaaagtgat gattatagag actgtaaacg taaagcaaag cgtttgatga
     2281 aacgattaaa ggaaactcaa atatatgcag ttaatccatt agctgaccag ttacaattgt
     2341 tttatcagtg tttacatggt aatgatttgt ttattaataa gtattggtta caaagaacta
     2401 cggcaacagg tatagcagaa aatttatttg gtgtgtctca aagtttggga acaaaaacag
     2461 gtttttatat agggcgtatt gataaattta ttcgttctgt ttcacgtgag gaagctgttg
     2521 caagttcaag ggatataatt ttatttagtt tattgttggc cgcaaaaggt attaaagggg
     2581 ctgtcagtga tagtccgcat gttttaatta ctggtcaaac tggtaaaggt aaatcatttt
     2641 tagcaaaatt attatggatt tatacatcat tttttaaagg tcaaatgttg tactgggatc
     2701 caaaatctga gtttgcagaa tggtttgaca gagtaacaga aagtaaggaa atgcaagaaa
     2761 agtatcccct atttattaat catttaaaaa catttaaata tgtaacattg aattatgaag
     2821 attcaaccaa ttatggttgt ctagacccta ttgtgttttt agatggtatt gaagctaatg
     2881 agatgttgaa atcagtgttt gatgaaatta aatcttttga aaattatcat catattgaaa
     2941 cggcaattta tcaagctatt tctgatacgg ttgaagaacg tgaaaatggt aaaaaagttg
     3001 gttcattaaa tgtaattgaa aaattacaga ataatgaaga tgagcatgta aaaaatgcag
     3061 gcgatttatt atttgaaaaa acgcaaaaca atattttaaa acttgttttt agtgatggta
     3121 cgaatccagc tttaaatatt caagaaaaag caactatttt acaggttaaa ggtttagata
     3181 tgcctaaagc agatgatgat acatcaagtt attcaacaag tgagaaaaat gggattacat
     3241 taatgttatt aattggtaag tttttagaga aattcggttc tcgtagagat gttcaaacga
     3301 ctatatttat tgatgagggt tgggctttta gtgcttcaag acaaggtaaa aaagttggta
     3361 agcgtataaa aagaacggga cgttctgaga ataattcact tgtatttatt acacaatcgg
     3421 taaaagataa agcagatgat gatggtggta actttggttg tcattttgca tttgatgaaa
     3481 aagatgaacg tgaggatatt ttaaaatctt taggcttaga atattcaaaa gaatctcctg
     3541 aaaatatgga aatgttgaaa gatttgaaga aaggtcaatg tatatttagt gatttttatg
     3601 gacgtgttgg aaaaatggtt gtacattgtc catttgagga aatgactgaa gcatttagaa
     3661 cacaagaaga ttctgcaagc tctaaagctg aagaaaaatt tgcgatgtag tttttagcac
     3721 atacaaaata gtatgtgctt tttattttaa aggagtggat ttgatggcaa tgaatgaaat
     3781 tgctttattt aaaggcgtac gtatacagcc ttatcagaag tatattgatt ttatgtttgc
     3841 aggtgtatta gcatttattt ttttggtata tcgtatatac attttgttta ttgattataa
     3901 atcagttaat aaaaaattgg atatatggac gttgtttgca tattttaaac cgcatttatt
     3961 gtatttatta attggtacag caattatatt tgttatatgt aagcttatcg ctcagctttt
     4021 aattcgtttt aaaacaaaag atttagctga aatgattgaa acacaaggtt ttcataatcg
     4081 tacagtaatc aagaaaacaa ccgagtatat ccctattgat tataaacaaa ctgaatatga
     4141 ctattatcca acgatgtttt ataaacgtgg taaaaaaact tttgttattc atattaaaaa
     4201 agacggttcg aggtttcaag ataattattt agagttagaa acgattattg aaccaatgtt
     4261 taattctgaa ttagtagaaa aaaagcatat tggtcgttat ttgagatatg aatttatgcc
     4321 acttaaatat aaaaagcgta ttgttatgaa tggcgaacaa gaatcaaata caatttttta
     4381 tgatacaaaa attgctatta cgcatcaaat tacttgggat tttgtgaaag cacctcatgg
     4441 attggttaca ggtataaccg gaggtggtaa aacatatttt ttattttatg tgattcgtga
     4501 gttattccgt agacattcag aagtacgttt attagatcca aaagtttcag atttgagctt
     4561 tatgaagcgc gttattggtg atgataaagt tgcagatact aaaggtcaaa tattaaaaca
     4621 attgagagaa gctaataatg aaatggaaga gcgttttaga ttaatgaatg atagttcgga
     4681 ttataaaatc gggaatgatt tccgtaattt tgatatgcgc ccctatttca ttatttttga
     4741 tgaggtaacg gcttttactt caactttaga taaaaaagaa ttacaagaaa tgaatgatta
     4801 tttaattaat attattatga aaggtcgtca agctggtgta tttatgtttt taacagcaca
     4861 aagaccagat gcagatgtga ttaaaggtaa tgtgcgtgat caattagggt tacgtgtgtc
     4921 tttaggtaat ttgtcaaatg atggttatcg tatgaccttt ggtcaaactg ataaagaatt
     4981 tcaaacgatt catgatagtg acatcggtcg aggttatatt tctatacttg gacaatataa
     5041 tgaaccaatt ttatttgatg cacctttaat ggaacaatat gattttgtgg aagatgtgaa
     5101 acaaatatta aataaggagt gatttgatga agcataaaag tgtgtatctt ttatgctttt
     5161 taattttgcc tattctgctt ttaacatctg tgagtgctta cgctgtttct aatccagttg
     5221 gagagccctc acatgcagtt aagccaaaaa ttgaaaagta tgatttatca cattatcgtt
     5281 caatatatag tgaaaaaggc gactggaatc cgtttggtca agaagaaatt tcacgacaaa
     5341 ttaataatgt gtcagatttt ttcttttcta tgactaagat tttagcaggt gttacagatt
     5401 atgcgattga aaatttattt caactagatg ttattaatga ttttgcggat aaaatcggcg
     5461 attttgttgg cgatatatat caaaagttat tgagtaattt ggcaataaca ttatttatta
     5521 ttgtttgctt taacgcattt attattttta gtgtacaagg taatgcaaga gaagcattaa
     5581 aacgggcgtt tttaattatg tgtttaattg gttttggtgt tggtatactt gcgaatgcag
     5641 gtaatattat acgtggtaca aacaatattg gtaaggactt aaataatata attatgaata
     5701 gtacatcttc tattgatggt aatattgatt atattcatga gaattcaggt atgaatcata
     5761 tacgtaatca attatttgat atgacgatat atagaacata tttagtaatg aattatggaa
     5821 ctgtagacga aaaagagata aaagcaaaag gtaaagaccg tattgataac attttaaaac
     5881 ttgattatga taaaaaatca gaaaaagaat tagatgatat tgttaaagat gaggttgaaa
     5941 agaaaaataa taaatatatg acgcaaggtt atgtatttca aaagttggct atttcagtga
     6001 ttggctttat tattacactt tttatgtcga tagtattttt atcaataagt tttgcgaagt
     6061 tgattttttc tacatttgct ttatttttat tcttattttt agtattcagt tggatagtga
     6121 gttttatacc aggatttgaa ttatctgttt tctctgcgtt tgcaaaaacg ttaggctata
     6181 ttattttaag tgcctgtatg acattcttat ttgtgatagt tggtttgtgt atcaagcttg
     6241 caaatagttt tattgatccg gatagtcaaa atgcttattt tttaaattca atatttataa
     6301 ttgtaatatt gtttgtaatg tataaaaaac gggctcaaat tatcaatttt gtaagtcgtg
     6361 gtaatattag tttttctcct agtgcaattg gtgctggtgt gatgaatcga acacaagaac
     6421 gttttaataa gattcgtcat gaacaagcgc aaaataagaa agctaaacga gaaaatcaaa
     6481 gagatgaacc cgccccacca cttcaaaatg ataatgattt aagaagaaga cagcagtata
     6541 aaccaatgcc tttattt
//
LOCUS       DATTQF010000135         6431 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.135, whole genome shotgun sequence.
ACCESSION   DATTQF010000135 DATTQF010000000
VERSION     DATTQF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.135"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(80..155)
                     /locus_tag="VB241_002241"
     tRNA            complement(80..155)
                     /locus_tag="VB241_002241"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Ala,seq:tgc)
     gene            complement(179..252)
                     /locus_tag="VB241_002242"
     tRNA            complement(179..252)
                     /locus_tag="VB241_002242"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Pro,seq:tgg)
     gene            complement(273..349)
                     /locus_tag="VB241_002243"
     tRNA            complement(273..349)
                     /locus_tag="VB241_002243"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Arg,seq:acg)
     gene            complement(355..443)
                     /locus_tag="VB241_002244"
     tRNA            complement(355..443)
                     /locus_tag="VB241_002244"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Leu,seq:taa)
     gene            complement(451..525)
                     /locus_tag="VB241_002245"
     tRNA            complement(451..525)
                     /locus_tag="VB241_002245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Gly,seq:gcc)
     gene            complement(559..631)
                     /locus_tag="VB241_002246"
     tRNA            complement(559..631)
                     /locus_tag="VB241_002246"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Lys,seq:ttt)
     gene            complement(638..713)
                     /locus_tag="VB241_002247"
     tRNA            complement(638..713)
                     /locus_tag="VB241_002247"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Thr,seq:tgt)
     gene            complement(730..805)
                     /locus_tag="VB241_002248"
     tRNA            complement(730..805)
                     /locus_tag="VB241_002248"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(770..772),aa:Val,seq:tac)
     gene            complement(818..932)
                     /gene="rrf"
                     /locus_tag="VB241_002249"
     rRNA            complement(818..932)
                     /gene="rrf"
                     /locus_tag="VB241_002249"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1490..2977)
                     /gene="lysS"
                     /locus_tag="VB241_002250"
     CDS             complement(1490..2977)
                     /gene="lysS"
                     /locus_tag="VB241_002250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP1245507.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(3030..3122)
                     /locus_tag="VB241_002251"
     CDS             complement(3030..3122)
                     /locus_tag="VB241_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245508.1"
                     /translation="MVNGKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3516..3992)
                     /gene="folK"
                     /locus_tag="VB241_002252"
     CDS             complement(3516..3992)
                     /gene="folK"
                     /locus_tag="VB241_002252"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP1245509.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISSIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPNLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVRRYK"
     gene            complement(3989..4354)
                     /gene="folB"
                     /locus_tag="VB241_002253"
     CDS             complement(3989..4354)
                     /gene="folB"
                     /locus_tag="VB241_002253"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP1245510.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLSEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4332..5135)
                     /gene="folP"
                     /locus_tag="VB241_002254"
     CDS             complement(4332..5135)
                     /gene="folP"
                     /locus_tag="VB241_002254"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP1245511.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMITVEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHRMFQVVAKYDAEIVLMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNFS"
     gene            complement(5351..6283)
                     /gene="cysK"
                     /locus_tag="VB241_002255"
     CDS             complement(5351..6283)
                     /gene="cysK"
                     /locus_tag="VB241_002255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP1245512.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
BASE COUNT         1967 a         1375 c          901 g         2188 t
ORIGIN      
        1 gtaacttata gttacgtttt ttggaattaa cgttgacata ttgtcattca gttttcaatg
       61 ttcatcgtgt aaaaataaat ggtggagact agcgggatcg aaccgctgac ctcctgcgtg
      121 caaagcaggc gctctcccag ctgagctaag cccccataaa atggaatttg agaagtaatc
      181 gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg
      241 agctacttcc cgttattcaa ttaatttaaa aatggcgcgc ccgataggag tcgaacccat
      301 aacctcttga tccgtagtca aacgctctat ccaattgagc tacgggcgct aaaatggtgc
      361 cgaggaccgg aatcgaaccg gtacggtgat ctctcaccgc aggattttaa gtcctgtgcg
      421 tctgccagtt ccgccacccc ggcaaaataa tggagcagaa gacgggattc gaacccgcga
      481 ccccaacctt ggcaaggttg tattctaccg ctgaactact tctgcattat tttgctaaat
      541 ataaaaatta aatcgtaatg agccatagag gattcgaacc tctgaccctc tgattaaaag
      601 tcagatgttc taccagctga gctaatggct caaaagatgg tgccggccag aggacttgaa
      661 cccccaacct actgattaca agtcagttgc tctaccaatt gagctaggcc ggctatatgt
      721 aagaataaat ggtggagaat gacgggttcg aaccgccgac cctctgcttg taaggcagat
      781 gctctcccag ctgagctaat tctccgattt gtcatttgcc tggcaacgtt ctactctagc
      841 ggaacgtaag ttcgactacc atcgacgcta aggagcttaa cttctgtgtt cggcatggga
      901 acaggtgtga cctccttgct atagccacca gacaaaataa cttacttatt tatgatacac
      961 gcttaattaa taaggcgcaa tagttaattt tacactttgt tgtgaacttt gtcacatcag
     1021 tttttagtgt gtttcgtctg tttttgacga cttttatata ttacttcatt caattgataa
     1081 agtcaacact tttaatcaac ttttttattc ttcaaaactg acatttctcg tgctctctaa
     1141 ttcataacac ttttagtatt ctatcataca catatgtaca aaaccataca cattctatac
     1201 aattttaaag tacttttata gctgattaag ataaattata ctaatataga aatttttgtt
     1261 ataagatatc tcaatgtcta tcattaacat ttctcatcag aactaatcta tatacgtatc
     1321 gtcattttca tctaacagct aactaccttg tttaacagtc aaaaatagca ccattatcgt
     1381 taactattac tatcaaaaac attcaaacac gaactaatta ccataattaa gaaaaccctc
     1441 gcatacacat gttgtataca cgagagctct tactaacaat caacgtcatt tatttttgtc
     1501 tcatataagg gaataataat acgtctctga ttgatggaga gtcagttaat aacataacta
     1561 atctgtcaat accgatacca agaccacctg tcggaggcat accatattct aacgcttcaa
     1621 tgtaatcttc atccatttca tgcgcttcat cattaccttg cgctttttca acaagttgcg
     1681 cttcaaaacg acctttttga tcaataggat catttaattc agtaaatgca tttgcatgct
     1741 ctctacctac aatgaacaat tcgaaacgat cagtaaatct aggatcttca ggatttttct
     1801 tcgctaaagg tgaaatttca gtcggatgac catagataaa cgttggctga ataagtgttt
     1861 cttcaacttt ttgctcaaag aattcattta aaatatgacc atatttcatt gtatctttaa
     1921 tttcaatacc atgttcctta gctaaagctt tagcttcttc atcactttta acttcataaa
     1981 aatctacacc agtagcttct tttacagcat caacaatatg caaacgagtc caagcagatt
     2041 ctaaatcaat cgtttcccca ttgtattgta cttttgcaga acctaatact tcattggcaa
     2101 tatgtctcac catagattct gttaaatcca taatgtcatg ataatctgca tatgcttcat
     2161 ataattcaat cattgtgaat tcagggttat gtctagttga tacaccttca ttacggaata
     2221 ctctaccaat ttcatatact ttttcaagtc caccgacaat taaacgtttt aaatgcaact
     2281 caatagcaat acgcatgtat aacgttgcat ctaatgcatt atgatgtgtt acaaatggtc
     2341 tagcagctgc tccaccagca atttggtgca tcataggtgt ttctacttcc aagaaacctt
     2401 tattatttaa ataattacgc atttcttgaa tgattttact acgattaata aatgtacgag
     2461 tgctatcttc gttcgtaatt aaatctaaat atctttgacg atatctctgt tcaatatcct
     2521 gtaaaccgtg gaatttatcc ggtaatggtc gcaatgattt agttagtagc gtgaatttct
     2581 tcgctttaac cgataattcg ccagtatttg ttttgaacat tacaccttca acaccaacga
     2641 tatcgcctaa atcagcattt ttccataaat caaattcgtc atcgccaact tgatctttac
     2701 gaacgtatat ttgaatttgt ccagctaagt cctgaacgtg tgcaaatcct gctttacctt
     2761 taccacgctt agtcattaat cgtccagcta tagcgacatg actatccgct tctttttcta
     2821 ccaattcttc tttagaatac tggtcccact cttctttcaa atcactagat aaacctgaac
     2881 ggtcaaattt agaaccaaac gggtctatac caagatcata taattcttgt aatttttgac
     2941 gtcgaaccaa catttggtca ttcatttctt ctgacataac tttctctcct ttaacttgtt
     3001 gtaatccttt agtattccaa ctggtacact caaacatttt cctttaaagt tttataaaat
     3061 atcagacatt tttgctcaac atacatctct ttcgatgttt ctaatacttt accattaacc
     3121 attgcacctt aaagttatga tgtggcatgt tttcttatat tcataacatc aattttatca
     3181 cttttttaat tttacaagag gttgtaaatg taataaagca atttctgttt tcgtttatga
     3241 attacttatt catttaaaat acgattatca ttaatacaac cctaagtaaa tgtataaaaa
     3301 ttatctttca caaacttcaa caaaagtcca aataaattac agcaatttat caaatattgc
     3361 ttactttgat tttatgaaat aacttaattc taacacatac taaatcatca tatactaatt
     3421 cgaaatcaaa tgcatttaga gataatcaaa atgccgaaac atctccaata atcaatcatc
     3481 tattcctaat aaatatgaat gttctcaaca atacattatt tatatcgcct tacactgtca
     3541 tcgacaaaaa ctaaatcttt cactttcaat ttcgaacgtg gttctatgac atttgctgct
     3601 atatcattta atgggattaa aacaaatgca cgttcattca ttctcggatg tggcaccgac
     3661 agatttggta aatctatcat ttcttctcca tacaacaaaa tatccacatc taaagttcta
     3721 ggaccccatc gttcctttct aacacggtgt aaacattctt ctgtcttcaa acaacattcc
     3781 aacagttgta atactgtgag tgttgtttga atctcaacac acaaatttaa aaagttaggt
     3841 tgctcagtat acccaactgg tgctgtttca taaatcgaag aaatattaga tacgttaata
     3901 ccatcatatt cattcaaaat ctttatagca tcgtttaact ggctttctct atcaccaata
     3961 ttactaccta atcctaagta tgcttgaatc atttattctc cctcacaatt tcgataccta
     4021 ctccatcata atgacccgga atcggtgggt tttctttagt gattctcact ttcgtttcca
     4081 ttacacgatt atattgtgaa tttatacgat ttgcaatacg ttcagctaga tgctcaagta
     4141 aattaacggc cttaccttcc ataattgatt taacctcttc gaacacttca ccataatgaa
     4201 ctgtatcaat aacattatca gtacgaccag cttcagataa gtctactttc aaagttacat
     4261 ccactttgaa aatttgtcct atttcatttt cagctgataa agcaccatga tatccataaa
     4321 agcgcatacc tttaagaaaa attgtgtctt gcattttcat tctcctttaa aaaatctata
     4381 cctttagcta atttagcatt caactcgaca ttatgaacgc gtactgctct aacgcctttc
     4441 ataataccat atgcagtcgt agctgcagtt acttcatctc tttcaaccgg tgttgtatca
     4501 taacccatca tctctttagt gaaacgtttc cggcttgtcg ctaataaaac tggatattct
     4561 gttgcaacaa gttcatccag tcttgccata acttcggctt cttcatttct agttttagcg
     4621 aaacctatac ctggatctag ccaaatttta tttgaaggta taccagctat tttagcttga
     4681 tgtgcttgtg ctaacaaaga tgttaacatt tcttcgacaa ccggttcatc acgattacca
     4741 ttgccattat gcattaaaac aatttccgca tcgtatttgg ctacaacttg aaacatgcga
     4801 tgatcataaa ggcctgccca ttgatcatta ataatatcaa cacctagctt taaacaagct
     4861 tcagcaactt cactacgaaa agtatcaacc gaaattttta catcaaaacc gacaatcgct
     4921 tcaacaactg gtaatactct attcaactct tcttccacag taatcatttc atggccgggt
     4981 ctcgttgaaa cacctccaac atctataatg tcagcacctt catccatcat cgctttcact
     5041 ctagttactg ctgattcaac attattaaat tttccaccat ctgagaatga atcaggtgtg
     5101 acgtttaata tgcccataat ttttgtttta gtcatgttaa ccacccttta attatgtatt
     5161 gtttaactct aataaaattt actcctgcga tttactagtt attagtatgt acgtttatta
     5221 tcttatcatt cactggatgt ttttatcaga aaaatgttta atttaacgct catattttta
     5281 aaattataca tggtagaaaa aaagagcaag ttatctcaaa aagatagctc actcttttaa
     5341 atgacattaa ttagtcatcg aaagaatata aaggtgttga taagtagcgt tcaccattac
     5401 tcggcaatac tgttactact gttttacctt ttcctaattc ttttgctttt tgaatggcag
     5461 cataaatcgc agcacctgat gaaatacctg ctaaaatacc ttcctcttta gcaactcgac
     5521 gagacatttc catcgctgta tcatttccta ctttaataat actgtcatag atttctgtat
     5581 tcaaagtacc tggaataaat ccagcaccta aaccttgtaa tttatgtgga cctggctcac
     5641 caccgctcaa tactggagaa gcctcaggct ctatagcaac aatttcgatg ttaggatatt
     5701 cttttttcag aactttacct acaccagata acgtaccgcc agtaccaaca ccagctagga
     5761 acgcatcgat agttttccct tcaaattgtt gtaataactc aggacctgta gttaactcat
     5821 gaacttcagg gttcgcaggg ttttcaaatt gttgtggctc gaagtaacca tgttcttctt
     5881 ttaattcttt agctttttta attgcacctt tcatcgcttc tgatccaggc gttaagacta
     5941 attccgcacc gtatgctttt aataaattac gacgctcttg gctcattgtt tcaggcatag
     6001 taaatactgc tttatatcct ttagcagcac atacaaatgc taaaccgata cctgtattac
     6061 cactagttgg ttctacaatt gtatcgccag gtttaatttt gccttctcgc tctgcttttt
     6121 caatcattgc taaagcaatt ctatccttta cagaaccacc tggattttga tattccaatt
     6181 ttacataaac atctgctgca ttgtcatcta ctacatttct caatttgact accggtgtac
     6241 cgccaataat ttgagtaata ttatctactg gtttttgtgc cattaagaac actccttttt
     6301 agttagttgt tgaaatctta gtttatttat cggatataga attattttat caaataaatt
     6361 ctagaaagca aacaaaatta ctaaggatta tacaactatt tagttctaca tttcactaaa
     6421 ccgctaaaaa t
//
LOCUS       DATTQF010000136         6390 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.136, whole genome shotgun sequence.
ACCESSION   DATTQF010000136 DATTQF010000000
VERSION     DATTQF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.136"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(158..664)
                     /locus_tag="VB241_002256"
     CDS             complement(158..664)
                     /locus_tag="VB241_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245513.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            777..1699
                     /gene="graX"
                     /locus_tag="VB241_002257"
                     /gene_synonym="apsX"
                     /pseudo
     CDS             777..1699
                     /gene="graX"
                     /locus_tag="VB241_002257"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
     gene            1715..2389
                     /gene="graR"
                     /locus_tag="VB241_002258"
                     /gene_synonym="apsR"
     CDS             1715..2389
                     /gene="graR"
                     /locus_tag="VB241_002258"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HEP1245514.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVIDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEIGMDSAIETKVGK
                     GYMAHE"
     gene            2382..3422
                     /gene="graS"
                     /locus_tag="VB241_002259"
                     /gene_synonym="apsS"
     CDS             2382..3422
                     /gene="graS"
                     /locus_tag="VB241_002259"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HEP1245515.1"
                     /translation="MNNLKWVVYFLKSRKNWIFWILFLNILMLGISLIDYDFPIDSLF
                     YIVSLNLSLTLIFLILTFFKEVKLYRHFEKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ILAHKDKVVDQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEENQERKQALLFEWSR
                     INSMLDTQLYITRLESQRKDMFFDYVSLKRMVIDEIQLTRHISQVKGIGFDIDFKVDN
                     HVYTDIKWCRMIIRQILSNALKYSENYNVDISTELIDQHVALIIKDHGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVDSVKDQLGIQLQVTSTIGKGTTVKLIFPLQNEI
                     VERMSEVTNLSF"
     gene            3569..4330
                     /gene="vraF"
                     /locus_tag="VB241_002260"
     CDS             3569..4330
                     /gene="vraF"
                     /locus_tag="VB241_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HEP1245516.1"
                     /translation="MTILEVKQLTKIYGNKKMAQEVLRDINMSVEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGHKLEKLSNKALSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKDTMLDRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLMKMNEEIKTTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGINYEF"
     gene            4320..6209
                     /locus_tag="VB241_002261"
     CDS             4320..6209
                     /locus_tag="VB241_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245517.1"
                     /translation="MSFNEIIFKNFRQNLSHYAIYLFSLITSVVLFFSFVALKYTHSI
                     NITETYPIIKEGSQVGSYFLFFIIVAFLLYANVLFLKRRSSELALYQTLGLSKFNIIY
                     ILMFEQLLIFSITAILGITIGIFGSKILLMIVFTLLGIKEKVPIIFSFRAVFETLLLI
                     GVAYFLTAAQNLILVLKQSISELSKHNQAKQMNHNKITFEEVILGILGIILIISGYYY
                     SLRIVHYYDAIGVLMFILFATVIGAYLFFKSSVSLVFKIIKSFKKGVISVNDVMFSSS
                     IMYRIQKNAFSLTVMAIISAITLSVLCFAAISRATLLSEIKYTSPHDVTIKDQQKANQ
                     LASELNNRKIPHFYNYKEVIHTKLYKDDLFDIKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGAIKDLVKHKKHGKAVIGTKKHHVDIKLRKDINKIYFMTDVDLGGPTFVLND
                     QDYQEIRKYTKPKHIVSQFGFDLKHKKEMLALEKATNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGISFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMAKGLKFKIA
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPIFIVMGLYICMYAIFAMTAYNHSKRT
                     IRHSI"
BASE COUNT         2320 a          830 c         1124 g         2116 t
ORIGIN      
        1 attagtggtg ttttattttg aatttaaatt tgataaatga ataagagtat acgcattcat
       61 ttaagattag atgacattga taagtatgag taatgtatta ggtaaccatt gaattcgttg
      121 ttatacgatt ctaatacaag attatgataa aatagcttta attaagaatt ttagccatca
      181 tatagtcatc gtaatattta ccatcgatga ataacttatc ttttaaaacg ccttcgattt
      241 gaaaatcggc acttttaaaa agctctaggg caggttggtt attgagtggt acatttgctt
      301 caattcggtg tatttgattg tttaaacacc aagccataat ggcatcaaga agtgcttggc
      361 caattccacg atgttgatat aatttcttta cacctaaatc aattttagca acatgtttaa
      421 tgcgttgaaa tggtgtcgta ttaacaaagg caaagcctac aagttgttca tcactttcag
      481 caacaaagat gactttatgt ggagaagtga tatattcttc taattgttta ctagccgatg
      541 tgacgctagg atcatattct cctggtgtgt agaacatata cggagattcg tcgtatattt
      601 tcgctaacat tgaaatgaaa ttttctacat ctttgatact aactctacgt ataatatggg
      661 ccataaaaag cctccagtat tttgaaattt taaaacattt gctactatta taatatatat
      721 ttaaccaaaa ggtagagtaa tatgtttttt ggttacagtt taaaggagta ttttagatga
      781 aacctaaagt tttattagca ggtggaacag ggtatattgg taagtattta agtgaagtga
      841 ttgaaaatga tgctgaactt tttgctatat caaaatatcc agacaataaa aaacagatga
      901 tgttgaaatg acttggattc agtgtgatat atttcattac gaacaggttg ttgccgcaat
      961 gaatcaaata gatattgctg tattctttat cgatccaaca aagaattctg ccaaaataac
     1021 acaatcatca gcaagagatt taacattaat cgcagcagat aattttggtc gagcagcggc
     1081 tatcaatcaa gtaaaaaaag taatctacat acctgggagt cgttatgata atgaaacaat
     1141 tgaacgccta ggtgcatatg gtacaactgt agaaacaaca aatttagttt ttaaacgttc
     1201 tttagttaat gtagaattac aagtttcaaa gtatgatgat gttagatcaa cgatgaaggt
     1261 agttttacca aagggatgga cattaaagaa cgttgtaaac cattttattg catggatggg
     1321 ttacactaaa ggaacttttg tgaaaacaga aaaatcacat gatcaattta agatatatat
     1381 taagaataag gtgcgaccac tcgcagtatt taaaatagta gaaacagctg atggaataat
     1441 aactttaatt ttattgagtg gaagtttagt gaaaaaatat acagttaatc aagggaagtt
     1501 agaatttaga ttaatcaaag aatcatcagt cgtttatata catctatacg attatatccc
     1561 tcgattattt tggccgattt attactttat acaagcacca atgcaaaaaa tgatgattca
     1621 tggctttgaa gttgactgcc ggattaaaga ttttcaaagt cgattaaaat caggagaaaa
     1681 tatgaaatat actaaatgat attgggtgat atggatgcaa atactactag tagaagatga
     1741 caatactttg tttcaagaat tgaaaaaaga attagaacaa tgggatttta atgttgctgg
     1801 tattgaagat ttcggcaaag taatggatac atttgaaagt tttaatcctg aaattgttat
     1861 attggatgtt caattaccta aatatgatgg gttttattgg tgcagaaaaa tgagagaagt
     1921 ttccaacgta ccaatattat ttttatcatc tcgtgataat ccaatggatc aagtgatgag
     1981 tatggaactt ggcgcagatg attatatgca aaagcctttt tatacaaatg tattaattgc
     2041 taaattacaa gcgatttatc gacgagtgta tgaatttact gctgaagaaa aacgcacgtt
     2101 aacatggcaa gatgctgtca ttgatctatc aaaagatagt atacaaaaag gtgatcagac
     2161 gatttttcta tccaaaacag aaatgattat tttagaaatt cttattacta aaaaaaatca
     2221 aatcgtttct agggatacaa ttattactgc gctatgggat gatgaagcat ttgtcagtga
     2281 taacacatta acagttaatg tgaaccgatt acgtaagaaa ctatctgaaa tagggatgga
     2341 cagcgcaatt gaaacaaaag taggaaaagg atatatggca catgaataat ttgaaatggg
     2401 tagtttattt tctgaaatca agaaagaatt ggatattttg gatactgttt ttaaatattc
     2461 ttatgttagg cattagtctt attgactatg actttccaat cgatagtttg ttttatattg
     2521 tttctttaaa tttaagctta acactgattt ttcttatatt gacttttttt aaagaagtaa
     2581 aattatatag acattttgaa aaagataaag aaatcgaaga aataaaacat aaagatttag
     2641 cggaaacccc attccaacgt catacagttg attatttata tcgtcaaatt ttagcgcata
     2701 aagataaagt ggtcgaccaa cagttgcaat taaacatgca tgaacaaacc atcacagaat
     2761 ttgtgcacga catcaaaaca ccagtgactg ctatgaaatt gttaattgac caagaagaga
     2821 atcaagaaag aaaacaagcg ctattatttg aatggtcacg tattaactcg atgttagata
     2881 cgcaattgta tattacgaga ttagagtcgc aacgtaaaga tatgtttttt gattatgtct
     2941 cattgaagcg catggttatt gatgaaattc aattaacaag gcatataagt caagttaaag
     3001 gtatcggatt tgatattgat tttaaagtgg ataatcatgt gtacacggat ataaaatggt
     3061 gtcgtatgat tattagacag attttatcaa atgctttgaa atatagtgag aattataatg
     3121 ttgatatttc aacggaatta attgatcaac atgttgcgtt aatcatcaag gatcatggaa
     3181 gaggtattag taaaaaagat atgcctcgaa tatttgaacg tgggtttacg tcaacggcta
     3241 acagaaatga aacgacatca tccggcatgg gtctatactt agtggatagt gtgaaagatc
     3301 aattaggaat acaattacaa gttacttcga ctattggaaa aggaacaacg gttaaattga
     3361 tcttcccatt acaaaatgaa attgttgagc gcatgtcaga agtgacaaat ctgtcatttt
     3421 aaacatgcgt tttgttactt agaattgata catcatgacc acttcaacga tataatcaaa
     3481 tagatgttaa ttaaatacaa aattgaaggt aagcgtgcca aattgaatat aaaggaaatg
     3541 aagttaagat aaatatagga gtgttaaagt gacaatatta gaagtaaaac aattaacaaa
     3601 aatatatggc aacaagaaaa tggcacaaga agttctgcga gatatcaata tgtctgttga
     3661 agaaggtgaa tttattgcca ttatgggacc ttcaggatct ggtaaaacga ccttattaaa
     3721 tgttttgagt tcaattgatt atatttcgca aggttctatc acactaaaag gacacaaact
     3781 tgaaaagctt tcaaacaaag cattatctga tattcgaaag catgatattg gttttatttt
     3841 tcaagaatat aatttacttc atacattaac tgttaaagaa aatatcatgt taccattaac
     3901 ggttcagaaa ttagataagg acacaatgtt agatcgttat gaaaaagtgg cagaagcatt
     3961 aaatatatta gacatcagtg ataaatatcc atccgaattg tctggtggtc aaagacaacg
     4021 aacatcagct gccagagcat ttataacatt gccttctatt atatttgccg atgaacctac
     4081 aggtgcactc gattcgaaaa gtacacaaga tttattaaag cgattaatga aaatgaatga
     4141 agagattaag acgacaatta ttatggtgac acatgatccg gttgcagcta gctatgcaaa
     4201 tcgagtagtg atgcttaaag atgggcaaat attcacggag ttatatcaag gtgatgatga
     4261 caaacatact ttctttaaag aaatcatacg tgtacaaagt gttttaggtg gcattaatta
     4321 tgagttttaa tgagattata tttaaaaatt tccgccaaaa tttatcacat tatgccattt
     4381 atcttttttc gctaattaca agtgtagttt tattttttag cttcgtagca ctaaaatata
     4441 cgcatagcat taacataact gagacatatc ccattattaa agaaggatcg caagtaggta
     4501 gttactttct attttttatc attgttgcct ttttgctata tgccaatgta ctatttctta
     4561 aacgtcgtag ttctgaactt gcattatatc aaacattagg tttatccaaa ttcaacatta
     4621 tatatatatt gatgtttgaa caattattaa tattttcaat aacagctata ttgggcatta
     4681 cgattggtat ctttggttct aaaattttat taatgatagt gtttacttta ttaggtatca
     4741 aagaaaaggt tccaattatt ttcagcttta gagcagtgtt tgagacacta ttattaattg
     4801 gcgttgctta tttcctaact gcagctcaaa atcttatctt agtattgaaa caatctattt
     4861 cagaactatc aaagcataat caggctaagc aaatgaatca taataaaatt acatttgaag
     4921 aagttatact aggaattcta ggtattattt taatcatcag tgggtattac tactctttac
     4981 gcattgtaca ttactatgat gcaattggcg tccttatgtt tatattgttt gcgacagtaa
     5041 ttggggcgta tttatttttt aaaagttcag tatccttggt atttaaaatt attaaaagct
     5101 ttaaaaaagg tgtaataagt gtaaatgatg tcatgttttc ttcatctatt atgtatcgta
     5161 ttcagaaaaa tgcattttca cttactgtta tggcaattat ttcagctatt actttatcag
     5221 tactttgttt tgctgctatc agtagagcaa ctttattaag tgaaattaag tatacgtcac
     5281 ctcatgatgt tacgattaaa gatcaacaga aagcgaatca gttagcaagt gaattgaaca
     5341 accgaaaaat tcctcatttt tataattata aagaagtgat tcatacaaaa ctttataagg
     5401 atgacttatt tgatataaaa gcaaaggaac catataacgt aacaattact agtgacaaat
     5461 atattcctaa tacggatttg aaacgtggtc aggctgattt atttgtagcg gaaggcgcta
     5521 ttaaagattt agtgaagcat aagaaacatg gtaaggcagt aataggaacg aaaaagcacc
     5581 atgtggatat taaattacgt aaagatatca ataaaattta ctttatgact gatgttgact
     5641 taggtggacc gacgtttgtg ttaaacgatc aagattatca agagataaga aagtatacta
     5701 agccaaaaca cattgtttct caatttggat ttgatttaaa acataaaaaa gaaatgttag
     5761 cattggaaaa agcaacaaat aaagttgaca aatctattga aacaagaagt gaagccataa
     5821 gttcgatatc gagtttaacg ggaatattgt tatttgtaac ctcattttta ggaattagtt
     5881 tcctaattgc cgtatgttgt atcatataca ttaaacaaat agatgaaact gaagacgagt
     5941 tagaaaatta tagtattctt agaaaacttg gatttacaca aaaagatatg gcaaaaggat
     6001 taaaatttaa aattgcgttt aattttgggt taccattagt cattgcactt tcacatgcat
     6061 actttacatc attagcatat atgaaattga tggggacaac gaatcaaata ccgatattta
     6121 tagtaatggg actatatatt tgtatgtatg ccatttttgc aatgacagct tataatcatt
     6181 ctaagcgtac aatcagacat tcaatttaat atatatatag cggctttcag taggctagtg
     6241 gattaagatt catgagcttt actgaaagct tttatttttg gatagttata ggtccgggca
     6301 cgacagtttt attgtatatg cttcaacttt gtaaattgta ttaaatgtga gaaaatagag
     6361 gcataggcat tgaaagaaat atgaattaag
//
LOCUS       DATTQF010000137         6257 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.137, whole genome shotgun sequence.
ACCESSION   DATTQF010000137 DATTQF010000000
VERSION     DATTQF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.137"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(15..302)
                     /locus_tag="VB241_002262"
     CDS             complement(15..302)
                     /locus_tag="VB241_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245518.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYVIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(337..1245)
                     /locus_tag="VB241_002263"
     CDS             complement(337..1245)
                     /locus_tag="VB241_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEP1245519.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVIQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(1348..2274)
                     /locus_tag="VB241_002264"
     CDS             complement(1348..2274)
                     /locus_tag="VB241_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEP1245520.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTVDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLVTDDSIEKIDKQLSLICASIPTYKTTYGQVSLEELDLPGKDREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLSESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            2501..2944
                     /gene="mgrA"
                     /locus_tag="VB241_002265"
     CDS             2501..2944
                     /gene="mgrA"
                     /locus_tag="VB241_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HEP1245521.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            complement(3070..4743)
                     /locus_tag="VB241_002266"
     CDS             complement(3070..4743)
                     /locus_tag="VB241_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HEP1245522.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRNNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGNKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFEALNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(4740..>6257)
                     /locus_tag="VB241_002267"
     CDS             complement(4740..>6257)
                     /locus_tag="VB241_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEP1245523.1"
                     /translation="FLAKIIRHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFK
                     VKHMLRQRVIYKNNGHPIGEQMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMF
                     FIHINTALIMLITAPFIPLFYIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKL
                     FNRTEQTEKHIYDDSTQFRTLTMRILRSAFLSGLMLEFISMLGIGLVALEATLNLVVF
                     HNIDFKTAAIAIILAPEFYNAIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLK
                     YEENQKPFIQLTDISFRYDDSDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAG
                     VYQPTIGTISINQRDLNIGILSQQPYIFSASIKENITMFKDIENNTVEEVLDEVGLLD
                     KVQSFTQGINTIIGEGGEMLSGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMI
                     QNVLFQHFKGTTMIVIAHRDNTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
BASE COUNT         2152 a         1116 c          864 g         2125 t
ORIGIN      
        1 tctcttttta atatttaaaa atgattaatg ccataaacat aaataattcc aaagaatgcc
       61 gcgccactaa gcactgttaa tataagactt agtaacaatg tgcgtttata atgtttaata
      121 aatgctatga acaaaatgac tacattatac gcaaagaaaa gtccgaataa tgtcattgtt
      181 attttcaaat cagaatagaa aatatttaat agtaatacaa tgactgcata aattacatac
      241 ggaataacta taaatttgcg ttcaaatctt aattgttcga aacgtttatc ttcgtttgtc
      301 atgatatgct cctttatgat ttaaaagtaa ataagtttac ggaatatctt gtccgataat
      361 agcggtgtaa atgtcaaacc acgactgatc atctaaatta agttgcagcc cttcgattgc
      421 ttgatcaata cgctttaact gacttgttcc aagtatcggc atgatacgat gcggtatttt
      481 tactaaccat gctatcatca cagctgtgtc actcacacca tatttgtcag ctattgattg
      541 aataactttc ataatacgtt gcgctttaat atcttccttg tcgaaaattt taccgcctgc
      601 aaaaggactc caagccatga tttgaacatg gttttgatac attgaatcca ttgttccatc
      661 ttgtaaacta tcaacgtgat atggcgataa ttctaattga ttgatgctaa tatgtagtct
      721 ttctttcata atatattgat ttaacaattg gtattgtgaa tgattaaaat tcgacacccc
      781 gaatgacttc aacttacctt gtttaacaag tttagttaat gcatcagcaa cttgttctgg
      841 atccatcaat ggtgaaggac gatgaatgag tagactatct aaataatcta cattcaaatt
      901 gattaatgac tgttcaacag atttcacgat gtgcttacta ctcaaatcat aacgatgtcc
      961 atttgtaaaa tcaaattgct tagaaggcaa aatgatacca catttcgtaa caatttgaat
     1021 tttatttctt aattcgggtg ataaatccaa agcattacca aacagtgatt cacattgata
     1081 atccccataa atatcagcat gatccatcgt tgtaatccca cgttcaacta attcatttat
     1141 aaaataattt aactctttcg cagtcatctt ccattcattt gcacgccaaa aaccttgtac
     1201 aagcctagaa aaatgaacat agtgattaat cattatttgt tccatatctc atccactcca
     1261 tccctcaatg tcacaaaata ttcacaataa aagtattaag aaattaatgt gctatttccg
     1321 ttaaattgaa acaacacatt ttctgattta cgtaaattgt agcatatcca attgattacg
     1381 tatgtgattt gtatctaaag tttcaccaat aataacaatc gttaatggta attgcatgcc
     1441 aattattcca taatctggta aaccataagc atattgaaat tcataaattg catttggttg
     1501 atctctaaat gatacataac ctttcaaacg tagaacagat tccgataatt tcattataaa
     1561 ttgataaaac aattgacgat caatcggacc tgtaaatgtg taagtcatac ttttaatccc
     1621 atgatgatgg tgatgatgcg acgatatctc tctgtcttta ccaggtaagt ccaattcttc
     1681 caacgaaact tgtccatagg ttgttttata agttggaata cttgcacaaa tcaaacttaa
     1741 ttgcttatca attttctcaa tactgtcatc agttacaaga tcaattttat taataataat
     1801 catgtcactt agtttcaact gatcttccat cagcgaaact gtattttcgg tatattgatg
     1861 acgttccaaa aatcgagtcg catctaatac accataaata attggctttt caaagaaatt
     1921 aacgatttgc ggatcttgac atgcaactag taattctaaa ggatgcgcaa tgcctgtcgc
     1981 ttctatgatg acatgattaa cgtccccttt taaagcaatg gcttttagtt catagacaag
     2041 ttcttgttta agatcgcaac aaacacaacc attaatcaat gaatggactt caatttcatt
     2101 tgaaatacta ttgctatcga tatcaacagt accgaattca ttcatgatga ttttaatttt
     2161 ctcatcattt tttaataatt cactaatata atgtgtcagt aacgtggttt taccactacc
     2221 caaaaatccg ttaattatgg atattcttat tttttcatct ttattatttt tcattttata
     2281 ttctcttttc tattacagtt atttttaaaa atgataacat atatattaaa ttatcttttt
     2341 gcttgattta atcaaaaata tcttctaaac tttaaacata ctttaatttt agcatggcat
     2401 tgaatcaaag aaagttgtga attaataaac aatcaacttt ttaatgatta ccaatccgaa
     2461 aagagaaaaa aacggatagt atgtctggag gagaacttta atgtctgatc aacataattt
     2521 aaaagaacag ctatgcttta gtttgtacaa tgctcaaaga caagttaatc gctactactc
     2581 taacaaagtt tttaagaagt acaatctaac atacccacaa tttcttgtct taactatttt
     2641 atgggatgaa tctcctgtaa acgtcaagaa agtcgtaact gaattagcac tcgatactgg
     2701 tacagtatca ccattattaa aacgaatgga acaagtagac ttaattaagc gtgaacgttc
     2761 cgaagtcgat caacgtgaag tatttattca cttgactgac aaaagtgaaa ctattagacc
     2821 agaattaagt aatgcatctg acaaagtcgc ttcagcttct tctttatctc aagatgaagt
     2881 taaagaactt aatcgcttat taggtaaagt cattcatgca tttgatgaaa caaaggaaaa
     2941 ataattaact tttgtcatga caattaaagt aatgtttaga atttattaag aatagaaaaa
     3001 caattagcac gcgtaagctt gttagttaaa aaactgcttg aaaggtttct tagcctatca
     3061 agcagttttt tatgcattat attgaatcat attccataaa gcaccttgat tagcgagtaa
     3121 ttgttgatag tttccctttt caactatttt accatttatc atcacaatga tggtctcaaa
     3181 acgtgacaat aaagttaaat cgtgtgtagc aacaattaat gtttctgcat gtgcttcaat
     3241 taaatccata actttcaaac tattttgttt atctaatgcc gttgttggtt catctaaaat
     3301 ccacgttgat gcagtatctt ttaataacat cctcgcaatc gctaaacgct gaatttctcc
     3361 gccagataat gtatgaccat ctaagtcaat ttgacgttct agtgccaaat gttctaaatc
     3421 taattgctta aatattgctt gcaccgcttc atctttttca tcggtaaata aattttgacg
     3481 tattgtacca tcaaataatt gttgagattg tagcaagaca tttaacgctt caaacttatc
     3541 tttgtcatct atttcaaaca tatccatatt ttcgaaacga atagagccac tatctaattg
     3601 atataaccct gccataattt gtagtaatgt actttttcct gaaccagaag gacccacaat
     3661 cgcaatcttt tcgcctctat taacattaaa attaatatcc gacaacacat acgtttgctg
     3721 gttccaatac ttaaaactag cgtccttaac ttcataaatg ttcgttgcat catacttatc
     3781 attaaggcgt ttatttccat tggtagaagg tacagatatc acttcattaa tatcgtgcaa
     3841 tgcttggtca gtatcggctt tataatacgc gacatttgtc attggtacag cttgttcaaa
     3901 taaagttaag accattaaaa ctatactcgt catataaata atatttagtt ggcctgcatt
     3961 aatttgaatt aatcctagaa ctaaactacc aaaaatcgaa agcatagcaa taatatttaa
     4021 tataaaatca taaatcgtta aaaagcgttg ctctttaagt tgtaatttat caaaatgatt
     4081 taatttagcc atcaaattat ttcgataatg attaatttga ttaaatcgac gtagttcatc
     4141 cataccagct ttataatcat aaaaatgatt taaaaatcga gcctgttcat tagctgcatg
     4201 tttctttaaa gtacgtgctt tttttgagct taaccaagga acaatgagta aagtaaacaa
     4261 catgctaacc ataatcaata gcgcatggcc gattgaaatg aacgccaata ctattactgt
     4321 aactagcgct gtcaaaccga tgacgactgg tggataataa acacgtaaat aaatattttg
     4381 taacgcctca acacgactaa tcatacgtga aattaaatca ctagaactca gtttacggta
     4441 aacattagga atgacattta ctaatttacc gaaaaactgt acccgaatat cacgtagcat
     4501 tgtaaatgta gctttatgag aaataaggcg ctctacgtat cgagtaatag ctcttaaaaa
     4561 cccaaacaat tttactgtaa cgactaaaat catcagagcg tatagtggcg caccaagtgc
     4621 actttgtgtc accatataac cacttaagaa aaacatggcg agcgcaacta aacttccaca
     4681 aacaccaaca actatagcta acaataaatc cttatctact tgaaatttta gtcgtgtttt
     4741 cataagtcat caccattttc tgttatattt actgaaatat tgcgatcatc agcaatcagt
     4801 cttccatttt ctatatacaa acgtcgttgt aaatggcgaa ttgtattatc tctatgtgca
     4861 atgacaatca tcgttgtacc tttaaaatgt tgaaacagaa cgttctgaat catgtgttct
     4921 gtttgaatat ctaaaccagt tgcaggctca tcaaatataa cgagatctgg cttcataact
     4981 aaaagacggc aaagttctat gcgtctcatc tgtccaccag ataacatttc gcctccttca
     5041 cctattattg tattaatgcc ttgtgtgaaa gattgaactt tgtctaataa acctacttcg
     5101 tctagcactt cttcaacagt attattttct atatctttaa acatcgtaat attctctttt
     5161 atagaagcac tgaaaatata tggctgttga ctaagtattc ctatatttaa atcacgctgg
     5221 tttatactta tagtacctat tgttggctga taaacacctg caataagatg tgtcaaagtt
     5281 gatttacctg ccccgcttgg acctacaagt gcaatttgat cacctttaaa tatttccaaa
     5341 tttaaatcat ttaataccaa tctatcagaa tcatcatatc gaaatgatat gtctgttaac
     5401 tgaataaatg gtttttgatt ttcctcatac tttaatagaa attcattatt attgttcggt
     5461 tgttctaaaa actcaaacac aacgtcactg gcaccttcac tttgttttcc agtatggaat
     5521 gcttgtccta agtccttaat tgcattataa aattcaggcg ctaaaataat cgcaattgcc
     5581 gcagttttaa aatcaatatt atgaaatact actaagttta gcgttgcttc caatgcaacc
     5641 aatccaatac ctaacatact tataaattcg agcattaatc ccgataaaaa agcactgcgt
     5701 aaaatgcgca ttgttaaagt tctaaactga gtactatcgt cgtaaatatg cttctctgtt
     5761 tgctctgtac gattaaatag ctttaacgtc actaaacctt tagcaatatt taaaaaccgt
     5821 tgactaaatt gattcaaata agtcatttga tcttttgact catctcgcgt tttcaaaccg
     5881 aaaataatat aaaacaaagg aataaatggt gcagttatta acataattaa tgcggtattg
     5941 atatggatga aaaacattgc aatgattatg atgagcggaa ccatcattga tttgaacact
     6001 tgaggcaaat aactcttata aaaaggtgct agaccatcaa tgttttctgt gagtatagtc
     6061 atttgttcac cgattggatg accattattt ttataaataa cccgctgtct aagcatatgc
     6121 ttaactttaa atgctaatgt atcacctaac cattgattta gaaattgcac agttgctctt
     6181 aaaagtaaaa cacctaataa aataaataat acaatccata aaccttgaaa ttgatgtctt
     6241 ataattttag ctaaaaa
//
LOCUS       DATTQF010000138         6252 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.138, whole genome shotgun sequence.
ACCESSION   DATTQF010000138 DATTQF010000000
VERSION     DATTQF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.138"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..609
                     /locus_tag="VB241_002268"
     CDS             <1..609
                     /locus_tag="VB241_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1245524.1"
                     /translation="NVENHKLLNNKERDLSNSAPLIYKTLSALYHNFHGFGLNIFDNH
                     QTFNAMHLYDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVI
                     ESETIMSNFEDSQVYINFKNNILNDKYLIVIETLDENSGNINHFISKELRDKYEWNPS
                     LLSKIDNYLKPAIEIKEHNFSNNSLNINVTFNALYIIKIGKK"
     gene            755..1114
                     /gene="sarX"
                     /locus_tag="VB241_002269"
     CDS             755..1114
                     /gene="sarX"
                     /locus_tag="VB241_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HEP1245525.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNDDD
                     ISKFNALFEDVEQFLNI"
     gene            1304..2020
                     /locus_tag="VB241_002270"
     CDS             1304..2020
                     /locus_tag="VB241_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1245526.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVDEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            2020..2493
                     /locus_tag="VB241_002271"
     CDS             2020..2493
                     /locus_tag="VB241_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP1245527.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYAVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            2585..2701
                     /locus_tag="VB241_002272"
     CDS             2585..2701
                     /locus_tag="VB241_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245528.1"
                     /translation="MLQLILMKIALNDFEKDKSVLFIFLKNHFYEEMCVLSN"
     gene            2846..3490
                     /locus_tag="VB241_002273"
     CDS             2846..3490
                     /locus_tag="VB241_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP1245529.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKIHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKN"
     gene            3608..4474
                     /locus_tag="VB241_002274"
     CDS             3608..4474
                     /locus_tag="VB241_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1245530.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMTSFVEEPKA"
     gene            4606..5826
                     /locus_tag="VB241_002275"
     CDS             4606..5826
                     /locus_tag="VB241_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HEP1245531.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            5823..>6252
                     /locus_tag="VB241_002276"
     CDS             5823..>6252
                     /locus_tag="VB241_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEP1245532.1"
                     /translation="MTIVLWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQ"
BASE COUNT         2238 a          786 c         1132 g         2096 t
ORIGIN      
        1 aatgttgaaa atcataaact gcttaataat aaagaacgag atttaagtaa tagtgctcca
       61 ttaatttata agacattaag tgcgctgtat cataactttc atggttttgg attaaacatt
      121 tttgataatc atcaaacatt taatgcgatg catctatatg ataaaaatgg atttaaaaca
      181 acactaggtc ttatattaga aaagtttatc gaatatgtct cgaaaccaaa atacgaaaac
      241 agttattatt ctatttttga tatagagaat tattattgtc tggttattta tgattggcga
      301 gtgatagaga gcgagacaat tatgagcaat tttgaagata gtcaagttta tattaatttc
      361 aaaaataata ttttaaacga taaatatcta attgtaatag aaacgttgga tgaaaatagt
      421 gggaacatta atcattttat ctctaaagaa ttaagagata aatatgaatg gaaccccagt
      481 ttactctcta aaattgacaa ctaccttaaa ccggcaatcg agattaaaga gcataacttt
      541 agtaataatt ccttaaatat taacgttact tttaatgcgt tatacataat taaaatagga
      601 aaaaaataac accttgatat gtattgcaaa atttaatttg catttgttgg taattttgac
      661 aacgtattaa aaacaaatga gaaagacaat ggcagaaaaa tttaagataa taatgacaga
      721 agcattgtct ttatatattt gggggtgcaa cattttgaat actgagaaat tagaaacatt
      781 gcttggcttc tataaacaat ataaagcatt atctgaatat attgataaaa aatataagtt
      841 gtcgctaaat gatttagcag tcttagattt aacgatgaag cattgcaaag atgagaaagt
      901 acttatgcaa tcatttttaa aaactgcaat ggatgagcta gatttaagta ggacaaaatt
      961 attagtttct ataagaagac taattgaaaa agaaagactt agtaaagtta gatcatctaa
     1021 agatgagcgt aaaatttata tttatttaaa tgatgatgat atatctaaat ttaatgcttt
     1081 atttgaagat gtagaacaat ttttaaatat ttaattgaaa ttgagtgtcg aaagcgtaga
     1141 aattgcttat cggcacattt ttaatttata catattttaa aactaagtaa cagattgaag
     1201 aaatcgtagt tcaataatgt taattgtgaa aatgtatata atcataaaaa aatcatgtat
     1261 aatatatgtt gttaattaaa cagttcgaaa gcgagatgac attatgggac gtaaatggaa
     1321 taacattaaa gaaaaaaagg cccaaaaaga taaaaataca agtagaatat atgcgaaatt
     1381 tggtaaggag atttatgttg cagcaaaatc tggtgaacca aatccagaat ctaaccaagc
     1441 tttaagattg gtacttgaac gcgctaagac atattcagta ccgaatcata ttattgaaaa
     1501 agcaatagat aaagctaagg gtgctggaga cgaaaacttt gatcacctaa gatatgaagg
     1561 atttggccca agcggatcaa tgcttattgt tgatgcgtta acaaacaatg taaatcgtac
     1621 tgcatctgat gtgcgtgctg cttttggtaa aaacggcggt aatatgggtg tatctgggtc
     1681 agttgcttat atgtttgacc atgtggcaac atttggtatt gaaggtaaat ctgttgacga
     1741 aatacttgaa acattaatgg aacaagatgt agatgtaaat gatgtgattg acgataatgg
     1801 attgacaata gtctatgctg aaccagatca atttgcagtc gttcaagatg cgcttcgtgc
     1861 agcaggtgtt gatgaattta aagttgctga atttgaaatg ttacctcaaa cagatattga
     1921 gctttctgaa gcggaccaag taacatttga aaaattaatc gatgcattag aagatttaga
     1981 agatgtacaa aacgtattcc ataatgtgga tttgaaataa tgaaatcagc agaacaatgg
     2041 attgatgaat tgcaacttga atcgcatcct gaaggtggtt tctatagaga gacaatccga
     2101 gaagtattga aagatggacg aagagcgccg tttagtagta tttatttttt gcttacagat
     2161 gacaatattt cgcattttca tcgaattgat gctgatgaag tatggtacta tcatgctggt
     2221 gattctctaa caattcatat gataaatccg gatggggaat atacgactgc aacattgggt
     2281 actgatatcc aaaatggaga tgtattgcaa tatgcagtgc ctaaaggaac aatttttgct
     2341 tcttcaatcg aaatttcaaa tacttatagt ttagtaggtt gtatgtgtca accagcattt
     2401 gagtttaagc agtttgaatt gtttaagcaa tctgaattaa ttacacaata tccgcatctt
     2461 aaatcagtaa tcgaaaaata tgctttaaaa taaaaagaat caatgaagtg atttgaaggt
     2521 tgttagtaaa cctttgagtc acttcatttt tgtatgtatt cttgattgaa tcagaataga
     2581 tttggtgctt cagctgattt taatgaaaat agcattaaat gattttgaaa aagataagag
     2641 tgtgttattt atatttttga aaaatcactt ttatgaagaa atgtgtgtac tatctaatta
     2701 atattgtcta ttttaagtaa attgaagcga gttgtagtaa aataataata aatattttca
     2761 tatgattaac aaaaactata aaactgtatc atgacatgac atcatccatg tagttttata
     2821 taataatgac aaggagttgg tgactgtgaa agtaaagtat atagataaac gtcactggcg
     2881 tcgcctaatt gatagggaat acacagaggt aaaagttaat aataataggt tcaagggtat
     2941 tataggctta gtcacgatga aaaaggttcg tgatccttta gaggtgacgg tagttggaca
     3001 aaatattatt gtcgcagatg acaattataa atggttgcaa atactacctg aaaagaaacg
     3061 ttatagtatt actgtaatgt ttgataataa aggcaatcca ttagaatatt actttgatat
     3121 aaatattaaa aatataacgc aaaaaggtaa tgcgcgtaca gtagatttat gtttagatgt
     3181 tttagcatta cctagtggtg aatatgagtt ggtagatgaa gatgatttaa tgtttgcatt
     3241 agaaagtgag caaattacaa aaaagcaatt tcatgaagca tatatgattg cgcatcaaat
     3301 tatggcagag ttagaaaatg attttaaagg attccaaaag aaaatcatgt actgctttaa
     3361 taaaattaat gcaaaggctc aaaaaattca tcaaaagcca caaaataaaa ctaattttga
     3421 aaaaagcaaa caaataaaac ctaagcaata taatcaaact aaaaatcacc aacaacaaaa
     3481 gaaaaactaa gtaattcaag ctgcagccat accaataaaa ttggtgaaaa ttattcgtta
     3541 gtaatgaact cttgaatgct cgtagtcatg aaagttcaat aattgaataa tttatggggg
     3601 ggaaattatg aagattgaag actatcgttt actaataaca ttagacgaaa cgaaaacgtt
     3661 acgtaaagcg gctgaaattt tatatatatc tcaacctgct gttacacaaa gactaaaagc
     3721 tattgaaaat gcttttggag tagatatttt tatcagaaca aaaaaacaat tgattacaac
     3781 aactgaagga acaatgatta ttgagcatgc ccgtgacatg ttgaaaagag agcgattatt
     3841 ttttgacaaa atgcaggcac atattggtga agtgaatgga acgatatcaa tcgggtgttc
     3901 ttcattgatt ggacaaacct tacttcctga agttttgagc ctatataatg cccaatttcc
     3961 taatgttgaa attcaagtac aagttggttc aactgaacaa attaaagcaa atcatagaga
     4021 ttatcatgtt atgataactc gtggaaataa ggtaatgaat ttagctaata cacatttatt
     4081 taatgatgat cattatttta tttttccgaa aaatagacga gatgatgtta caaagttacc
     4141 atttatagag tttcaagctg atccgattta tataaatcaa ataaaacaat ggtataacga
     4201 taatttagaa caagattacc atgcaactat tacagtggat caagtagcaa cttgcaaaga
     4261 aatgttgatt agtggtgtag gtgttacaat cttgccagaa atcatgatga aaaatatcag
     4321 caaagaacaa tttgagtttg aaaaagtaga aattgataat gaaccgctga ttcgttcgac
     4381 atttatgagt tatgatccga gtatgttgca attaccacaa gttgattcat ttgtaaatct
     4441 tatgacgagc tttgttgaag aaccaaaggc gtagtcttag actaatttaa ggttagtatt
     4501 taattttaag atatacggtt aaattgaaca cagttggatg ctaatgcacc aacagcaaaa
     4561 gagcccctaa ttaataaatt aaaaggggct ctaagaagtg gggttatgga tatgtttgca
     4621 gcgttattac aaataaagaa ttataaactc tttgttgcta atatgtttct attaggtatg
     4681 ggtattgcgg ttacggtccc atatcttgtt ctttttgcaa ctaaagattt gggtatgaca
     4741 acaaatcagt atggattact tctagcatct gcagcgatta gtcagtttac agtaaattca
     4801 attattgcta gattttcgga tacgcatcac tttaatagaa aaattattat tattctcgca
     4861 ttattaatgg gtgcgcttgg tttttcaata tacttttttg tagatacaat ctggttattc
     4921 atattactat atgcgatttt ccaaggatta tttgcaccag caatgcccca actttacgca
     4981 tctgctagag aatctatcaa tgtttcaagc tctaaagata gagcccaatt tgccaataca
     5041 gtattacgtt caatgttctc attgggcttt ttatttggtc catttattgg tgcgcaatta
     5101 atcggattaa aaggctatgc tggattgttt ggtggaacaa taagtatcat tttatttact
     5161 ttagtacttc aagtgttttt ctataaggat ttaaacatta aacaccctat tagtacgcaa
     5221 caacatgttg aaaagattgc tcctaatatg tttaaagaca aaacgctttt attaccattt
     5281 attgcattta ttttattaca cattggacaa tggatgtata cgatgaatat gcctttattt
     5341 gttactgatt atttaaaaga aaatgagcaa catgtcggtt atttagctag tttatgtgct
     5401 ggtttagaag tgccatttat gatcattctt ggcgttttat catctagatt acagactcga
     5461 acattgttga tttatggagc gatttttgga ggtttattct acttcagcat tggggtattt
     5521 aaaaacttct atatgatgtt agcaggacag gtgtttttag ctattttctt agcggttctt
     5581 ttaggaattg gtattagtta tttccaagat atcttaccag atttcccagg atacgcctca
     5641 acactatttt ctaatgcaat ggttattgga cagttaggcg gtaacttatt aggtggtgct
     5701 atgagtcact gggtaggttt ggaaaatgta ttttttgtat cagcagcatc aatcatgtta
     5761 ggtatgatac ttatattctt tactaaaaat caaaaaatta caaaagagga tgtgatatca
     5821 acatgacaat tgttttatgg ctacttatca tcgctgcctt catgttagca tttgttgggt
     5881 tgattaagcc gattattcct tctgttttag tattatgggt tggctttcta atctatcaat
     5941 ttggctttca taatcagcat ttatcatggg tgttttatgt atctatggca ttgctaacaa
     6001 tattaatttt atgtgcggac tttttagcta ataaatattt tgtgaatcgc ttcggtggtt
     6061 ctaagtttgg agagtatgca gctttaattg gtgtcgttat tggatgtttt gttttaccgc
     6121 catttggaat tattatcatt ccatttattt tggtattcat agttgaatta atacaaggat
     6181 attcatttga aagagcagtt aaagtaagta taggttcaat cgtagcattt ttaacaagta
     6241 gtatagctca ag
//
LOCUS       DATTQF010000139         6120 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.139, whole genome shotgun sequence.
ACCESSION   DATTQF010000139 DATTQF010000000
VERSION     DATTQF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.139"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            134..1111
                     /locus_tag="VB241_002277"
     CDS             134..1111
                     /locus_tag="VB241_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245533.1"
                     /translation="MIKFKNVTKRYDKHVAVDNISFDINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQQIEVLYQHLSESERVIVMQEDGVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            1104..2618
                     /locus_tag="VB241_002278"
     CDS             1104..2618
                     /locus_tag="VB241_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP1245534.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYDQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKIKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            2858..3916
                     /gene="hisC"
                     /locus_tag="VB241_002279"
     CDS             2858..3916
                     /gene="hisC"
                     /locus_tag="VB241_002279"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEP1245535.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLDGILKEVNDDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPSHVPVLIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNGKMLEVLSNFKYE"
     gene            4075..4617
                     /locus_tag="VB241_002280"
     CDS             4075..4617
                     /locus_tag="VB241_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HEP1245536.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(5169..6086)
                     /locus_tag="VB241_002281"
     CDS             complement(5169..6086)
                     /locus_tag="VB241_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP1245537.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKAKDYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILSANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPTMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
BASE COUNT         2197 a          878 c         1100 g         1945 t
ORIGIN      
        1 tattattcat tattttgtta aaaaagttca aggataaatc atttaaattt gaaaattgta
       61 ataaacgtgg ttaaataggg aatacataca tataatctcg gttttatgct atgagaaaaa
      121 aagaggtggc aaagtgatta agtttaaaaa tgtaactaag cgttatgaca aacatgttgc
      181 tgtcgataac attagtttcg atattaatga gggtgaattc tttgtactaa ttggaccttc
      241 aggttgtgga aaaactacga cattaaaaat gattaatcga ctcattcact taagtgaagg
      301 ttatatttat tttaaagata aaccaataag tgattatcca gtatacgaaa tgcgttggga
      361 tattggatac gtattgcagc agattgcatt attcccacat atgacaatca aagaaaatat
      421 tgcacaagtg ccacaaatga aaaagtggaa agaaaaagat atagataaaa gagtagatga
      481 attacttgaa atggttggat tagaacctga aaaatataaa aacagaaaac ctgatgaatt
      541 gtcaggggga caacgacaac gtgtaggagt tatacgtgcg ttagcagctg atccaccagt
      601 tattttaatg gatgaaccgt ttagtgcatt agacccaatc agccgagaaa aacttcaaga
      661 tgatttaatt gaattacaaa ctaaaattaa gaagacaatc atatttgtta cacatgatat
      721 tcaagaggcg atgaaacttg gtgataagat ttgtcttttg aatgaaggtc atattgaaca
      781 aattgacaca ccagaaggat ttaaaaataa tcctcaaagt gaatttgtta aacaatttat
      841 gggtagtcat ttagaagatg atgcgccatg tgttgaagag aatgcgatta tccgtgactt
      901 ggatattatg aaaccaatcg atgaggttac atctatgagc gcttatccaa ttgtttatga
      961 caatcaacaa attgaagtat tgtatcaaca tttatcagag agcgagcgtg tcattgtcat
     1021 gcaagaagat ggcgtaggtc aatatgttat tgataggaaa gatatcttca aatatttgtc
     1081 ccagaaaaag gaggtagctc aacatgacta actttttcga catattgagt gagcgtaagg
     1141 ggcaactatt ttcgacaatg atagaacata ttcaaatatc atttatcgca ttattgattg
     1201 caactgctat tgcggtacca ttaggtattt tattgacgaa gactaaaacg atatctgaaa
     1261 tcgtaatgaa tattgcggca attcttcaaa ccataccatc gttggcatta ttaggtttaa
     1321 tgattccttt atttggtatc ggtcgtgtgc cagcaattat cgcacttgta gtgtatgcat
     1381 tgttaccaat tttaaggaat acgtatactg gaattaaaga agttgatcca tcgctcattg
     1441 aagcggctaa aggtataggt atgaagccat ttagacgttt aactaaagtt gaacttccaa
     1501 tagcaatgcc tgttataatg gctggtgtaa gaacggctat ggtattaatt ataggtacag
     1561 caacactagc agcattaatt ggtgcaggcg gactaggaga tttaatttta ttaggtatag
     1621 accgtaacaa tgcatcattg atattattag gtgcaattcc agcagcctta ttggcaatta
     1681 tatttgattt aattttaaga tttatggcta aattatctta taaaaagtta ttgatgacgt
     1741 taggtgttat agtgatgatt attatactgg ccatcgctat tcctatgttt gcacaaaaag
     1801 gtgataaaat tacgttagct ggaaagcttg gctccgagcc ttcgattatt acaaatatgt
     1861 ataaaatttt aatagaagaa gagaccaaaa atactgtaga agtgaaagat ggtatgggca
     1921 aaacagcatt tttatttaat gctttaaaat ctgacgatat agatgggtat ttagagttta
     1981 ctggaacagt tttaggtgaa ttaacaaaag aaccattgaa gtcaaaagaa gagaaaaaag
     2041 tatatgatca agctaagcaa agtcttgaaa agaaatatca aatgactatg ttaaaaccaa
     2101 tgaaatataa caatacgtat gctttagctg taaaacgtga ttttgctaaa caacataata
     2161 tacgtacaat tggtgattta aataagatta aagatcaact taaaccagga tttacattgg
     2221 aatttaatga tcgtccagat gggtacaaag ctgttcaaaa ggcttataat ttaaatttag
     2281 ataacatacg tacaatggaa cctaagttga gatatcaagc gattaataaa ggtaatatta
     2341 atttaattga tgcatattca actgacgctg aattaaaaca atatgatatg gttgtgttaa
     2401 aagatgataa gcacgtattt ccaccatatc aaggagcacc attatttaaa gaaagctttt
     2461 taaagaaaca tccagaaatt aagaaaccgt taaacaaact agaaaacaaa atatctgatg
     2521 aagatatgca aatgatgaac tataaagtaa cagttaaaaa tgaagaccca tatacagttg
     2581 cgaaagatta tttaaaagca aaagggttaa tcaaataacg accaacgcca cataagatga
     2641 ggggcacgcc attatatctt atgtggcgtt gctatattta catctatgtt tatgttcaat
     2701 ttaaacatgc aatactgatt aaaaaatatg acatgttaaa cacaatgtaa gctagtaatg
     2761 tgtgaaaata gtagcattgc attttagaaa catagagcga tataatgaat ataagttttt
     2821 taaaatttca gttaattcta aggaggttgt ttttattatg aaagaacaac ttaatcaact
     2881 atcagcatat cagcctggtt tatcaccaag ggcattgaaa gaaaagtatg gcattgaagg
     2941 agatttatat aaacttgcat caaatgaaaa tttgtatgga ccatcgccta aagttaaaga
     3001 agcgatatca gcacacttag atgagttata ttattatcct gaaacaggat caccgacatt
     3061 aaaagcggcg attagtaaac atttaaatgt agatcaatca cgcattttat ttggtgcggg
     3121 attagatgaa gttatattaa tgatttctag agcagtatta acgccagggg acactattgt
     3181 tacaagtgaa gcgacattcg gtcaatatta tcacaatgcg attgttgaat cagctaatgt
     3241 gatacaggta cctttaaaag atggtggctt cgacttagat ggcattttaa aagaagttaa
     3301 tgatgacacg tcattggtat ggttatgtaa tccaaataat cctacaggta cgtattttaa
     3361 tcatgagagc ttagattcgt ttttatctca agtaccatca catgttccgg tactcataga
     3421 tgaagcttat tttgaatttg tgacagcaga ggactacccg gatacacttg ctttgcaaca
     3481 aaaatatgac aatgcattct tattacgtac attttcaaag gcgtatggat tagcgggttt
     3541 acgtgtagga tatgtggtag caagcgaaca tgcgattgaa aaatggaaca tcattagacc
     3601 accatttaat gtgacacgta tatctgaata cgcagcagtt gcagcacttg aagatcaaca
     3661 atatttaaaa gaggtaacac ataaaaatag tgttgaacgt gaaagatttt atcaattacc
     3721 tcaaagtgag catttcttac caagtcaaac gaattttata tttgtaaaaa caaagcgggt
     3781 aaatgaactt tatgaagcac ttttaaatgt agggtgtatt acgcgaccat ttccaactgg
     3841 tgttagaatt acaattggtt ttaaagaaca aaatggtaaa atgttagaag ttttatcaaa
     3901 ctttaaatac gaatagtaag tgggcaccac atggtatgaa tatgtgacaa aatgcagtgt
     3961 gaatgatgag gtgagagtat tcagtgtaaa aagcaacaat agatgatatt gttttgtatc
     4021 aattgctttt ttgctatact gaatcaatac tgatattttg aggagaagat taaaatgacc
     4081 cgtaaatcaa tcgcgattga tatggatgaa gtattggcag atacattagg agaaatcatt
     4141 gatgctgtca attttagagc ggatttaggt attaaaatgg aagctttgaa tggtcaaaaa
     4201 cttaaacatg ttattcctga acatgatgga ttaattacag aagtattaag agaaccaggc
     4261 ttcttcagac accttaaagt gatgccgcat gcacaagaag ttgtaaaaaa attaactgaa
     4321 cattatgatg tatatattgc tacagcagca atggatgtac caacatcatt cagtgataaa
     4381 tatgaatggt tactagagtt ctttccattc ttagatccac agcattttgt tttttgtggt
     4441 agaaaaaaca tcgttaaagc tgattattta atagatgaca atcctagaca gcttgaaatt
     4501 tttactggta caccgattat gtttacagca gtgcataata ttaatgatga tcgatttgaa
     4561 cgcgtaaata gctggaaaga tgtagaacag tattttttag ataatattga gaaataaaaa
     4621 tatgtcactc gaagcctttc atgtagcaaa taaaatgcat atactggggc cccaacaaag
     4681 agaatttcga aaagaaattc tacaaacaat gcaagttggg gcggggcccc aacataaaga
     4741 aatacttttt ctttagaaat tagtatttct tatgcatgag tgtaactcat gcaatcctat
     4801 ttttaagtac acattagctg tgactaatga taaagaatcg ctacataatc aaccattagt
     4861 cgttctttat catttccatc ccactctcaa taaatgttag tctatctcat tattataaat
     4921 cggatgaatg tgttaatcta tggcagatta cacttcatcc gattttttat agaatttgaa
     4981 aaaggcacat aaaccactat gatttaaaat acaacatcaa tcattttagt ggcatgcgcc
     5041 aaaattatat gtctgttttt gaaacagggt aatagcttaa agctaatacc aacaaatatt
     5101 acgtacgttg aatcttatga ttacacttta aacctaatat aatatcgcgt taatatcatt
     5161 ccggatgctt acaaatcatt gacagtaagc aactgaatgg catttggtat aacctcaata
     5221 tcaataggtg tttctaatga aatttcgcca tcaatatcaa ctttcattgt tggatctgtt
     5281 gtaagtgaaa tctttttacc aggtatatgc tcaatacctt gagtaatttc attccaattc
     5341 atactatcac gctttttaaa aatatcattt aaaatactga aactttgttc attaaaaatg
     5401 aaagtgttca gttcaccatc ttgaggagac aaatcagtca atggtattcg actaccacca
     5461 atgaatggac catttgctga tagtatcatg gtcgtttcgc cagaatatgt cttatcatca
     5521 attgataatt gataattaaa ttgtgttgga tttagcagtg ttttgacagt tgatccaata
     5581 taactcaatt taccaaatat atcttttgaa ccatcttgta cgttttcagc gttttgaaca
     5641 attagaccta agccaacaaa gttgagtgca tattgattat ttattttaat tacatcgtat
     5701 gtaccaactt gtgcagaaat catttgttca ctagcttgtt tatgattagg tgctatattt
     5761 agcgtttttg taaaatcatt aaaagtaccg cctggtaaaa tgccaatagg gagttgaagg
     5821 tcgtgtgtca taacaccatt tataagttcg ttaaccgtgc catcaccgcc aagaataaat
     5881 aatatatcta catcttttgc ataatcttta gctttaattt cttggcaata tttaattatg
     5941 tcaccttcgt tttcactcaa ttgaatagaa agatgcttac aaattgaact taatgccgtt
     6001 gtaacttccc cgataccttg attaatattt tttaatccac tgtgttcatg gtaaaagagg
     6061 acaccatgtg tatatttatt ttccatagtt tagcctactt tctaaaaatt ggttcattaa
//
LOCUS       DATTQF010000140         6005 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.140, whole genome shotgun sequence.
ACCESSION   DATTQF010000140 DATTQF010000000
VERSION     DATTQF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.140"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            218..532
                     /locus_tag="VB241_002282"
     CDS             218..532
                     /locus_tag="VB241_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1245538.1"
                     /translation="MTYKELATYLKILSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            532..1823
                     /gene="arsB"
                     /locus_tag="VB241_002283"
                     /pseudo
     CDS             532..1823
                     /gene="arsB"
                     /locus_tag="VB241_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
     gene            complement(1910..2764)
                     /locus_tag="VB241_002284"
     CDS             complement(1910..2764)
                     /locus_tag="VB241_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HEP1245539.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3040..3264)
                     /locus_tag="VB241_002285"
     CDS             complement(3040..3264)
                     /locus_tag="VB241_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245540.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            3463..3933
                     /locus_tag="VB241_002286"
     CDS             3463..3933
                     /locus_tag="VB241_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEP1245541.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSNQSLSSFLFMRLNFYLIDLFRQQKRLNDIILCENHSPILTEQPTYFNEYDLRL
                     QDVCALLNHRERQWLMLYLEGYKQYEIADIMSLSTSTIKLIKASVKRKCQRFFK"
     gene            4046..4489
                     /locus_tag="VB241_002287"
     CDS             4046..4489
                     /locus_tag="VB241_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HEP1245542.1"
                     /translation="MQDISTKHLLYIQTATSNHLETKCVFLYHEYNLEFPITKAIPYY
                     AKLHFTSASILIDAAKSILNINKLVPIYLNPKTILFPIKQKRAPIQTYINAHYIIGMT
                     EMGNSTMLHFKNGKTLEVDEPMSLISKKCYESLALKHFIENTNDY"
     gene            complement(4476..4919)
                     /locus_tag="VB241_002288"
     CDS             complement(4476..4919)
                     /locus_tag="VB241_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245543.1"
                     /translation="MNKASFDKKVKKQLWFLNKNEKQALDQRLSSITDKDNVNFNKPI
                     TFANTYLRENVFRSKETKSYSIFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVNPK
                     VELSTTVVILTIIGAILLMLVSIYLIKITTSYFTKKLLEHKFNSH"
     gene            5217..5852
                     /locus_tag="VB241_002289"
     CDS             5217..5852
                     /locus_tag="VB241_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1245544.1"
                     /translation="MQKFKDLFYDDLSVARGNYFLTLLSAFLITIILLIGFVVSDTHI
                     LYGVFIVLIGIVLLRLFKINLLSFKKLTMSQVIYIIGGALLIYGLDHLYLHFHDVPTN
                     EQELDRELQHMPLYMSIVTIAIIPAIVEEIVFRGMIIRVVFRKHLFIGLVVSSLVFAS
                     LHESDTWIGYLPYLYSGVIFGLIYLKTKRLEVVILIHFINNLSSLLILIWG"
BASE COUNT         1997 a          977 c          803 g         2228 t
ORIGIN      
        1 aaggtgattg gccatacact caagatatgg ctaatcacct ttttcaatgc agaataaaga
       61 gtatattttg caaaatcaat atttataatt tatctttaat acccttttaa tactaaaacc
      121 aaatttaaat tacatagtca tatcgtgaaa tataccaaag aaacatagat atttatctat
      181 atagatgata atctatttaa cgtctaggag gtatgttatg acgtataaag aactagcaac
      241 atatttaaaa attttatcag attcaagcag attagaaata ctagatttac tttcttgtgg
      301 agagttatgc gcttgtgatt tgttagaaca ttttcaattc tctcaaccca cacttagcta
      361 tcatatgaaa gcattagtaa aaaccaactt agttacgaca cgaaaaatcg gaaataaaca
      421 tttataccag cttaatcata atatttttga gtccgtaatt aataacttgt caaaagttca
      481 tacctctaac caacgatgta tttgtcataa ccttaagact ggtgaatgct aatgatgaca
      541 actttagcga cattcatttt tctagtaact ttattatttg tattatggca acctaaaggc
      601 ctagatattg gatttactgc attagcaggt gcctttattg ctgttattac tggtgttgta
      661 agtttttccg atgttttcga agtaacaggt atagtctgga atgccacttt aacttttgta
      721 tcagtcattc ttatttcatt aatattagat aaagttggat tattcgaatg gtcagctatt
      781 cacatgcttc atgcttcaaa aggtagtggc ctaaaaatgt tcgtttatat catattattg
      841 ggtgccgtgg ttgctgcatt tttcgcaaat gatggcgcag ctttaatctt aacgcccatt
      901 gtattagcaa tggtaaaaat ataggtttta gtaagcgggc catatttcca tttattattg
      961 caagtggttt tattgctgat acaacgtctt tacctttaat cgtgagcaat ttggtcaata
     1021 tcatttctgc agattacttt aacattagtt tctctcaata tcttagtaga atgattatcc
     1081 ctaatttatt ctcgttatta gctagccttc tagttttgtg gttatatttt agaaaagcga
     1141 ttcctaaatc atttgatgca aatcacataa aaaaacctat agatgccatt aatgatttaa
     1201 agctatttaa aatttcatgg attgttctag ttatattact tttcggctat ctaatcagtg
     1261 aatttactaa aattccggta tcaattttca ccggaatcat tgcttttata tttctaatat
     1321 tggctcgtaa atcaaatgct gtaaatatta agcaagtcat taagggcgca ccttggaata
     1381 tagtattatt ttcaattggt atgtatatcg tcgtattcgg cttaagaaat gctggcatta
     1441 ctttaatatt agctaaaata ttagaatata tttccaatta cggtctattt agcactattt
     1501 tgggaatggg cttcatttca gcatttttat catcaataat gaataatatg cctacagttt
     1561 taatagatgc gattgctatt ggtcaatcaa atgtccatgg catgttaaaa gaaggcctaa
     1621 tttatgcaaa tgttatcggt tctgatttag gtccaaaaat tacaccgata ggctctttag
     1681 ctacattact gtggttacac gtcttaacac aaaaagatgt taagatttct tggggcacat
     1741 actttaaaac tggtatcatc attacaattc cagtactatt tttcacactt ttaggattat
     1801 atctaacact tatactattt taagtaataa aaagacgctt tctatttatt gcgatagaaa
     1861 gcgtcttttt gtacttgagc aagtcatatc ataacttaac tctaacaaat tacttattca
     1921 aatgtttact gtcatcttta tacacaaagt atttgaagta ttttccttca gtcttcatgt
     1981 tcttataaaa gtcagcgata attgttgcat tactttctgc ccacttaata tctgtagcat
     2041 attgatgttc tcctggattt tttggattcc atctcatact atacaatgta ttttgatctg
     2101 tgcttgataa gaagtgctta tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg
     2161 tatcccaacc atgcttttta gcatattctg cacctgtttt aattgggtct ttatcaagag
     2221 ctcccactcc atagaaattg tagtattttt taccatcaat ttcgacacca ttagctaatt
     2281 cacttttaac tgcgccagtt tctaataatg catgtgaaat taaataaact tcgttaacgt
     2341 gcttatcttt agcagctttt aagaaatcat ccgtatgttt caataacgtt ggtctatcga
     2401 ctaacatacg tttaattcta tttttatcaa tcccttgata ctttgataaa tctaaaaatt
     2461 gatatttttg cttttcatta tcgataaaag taccgctatc cattgcactt ttaatttcag
     2521 ttgcagatgc atctctccat gcatcattct ttttatttga tacctgttga ctcgtataat
     2581 tatttatttg tttctttgct gcatcgttta atgtaacatt taacttttca atcttaatgt
     2641 cggatttaac atgtttgaaa aatatctgat cggatatcat tgagaaaaat aaaaatgaga
     2701 caacagcaaa tatgacaaca agtcctatta ttccaaaaat agaacctttc ttgtgtttat
     2761 tcatatccac acctcttagg tcattgttgt tatacattca gtttgactac gataataaag
     2821 tttaacatta ttaaaaccgt ttaacaattt tatctatgca ttgttacata ttcataaata
     2881 aacagtaatg taaatgaaac gttttaaacg atttatcaac attactttac gttgattcac
     2941 gaatcataaa tgttttatct ttttgtataa cctttgaaaa cctttatatc aataatctta
     3001 ttattcacgt attaaaaaac aaaaaattct cttgttcgtt tacttagttt cttttttata
     3061 atccatcata ataaaacttg ctgcatttga tctattttta taatagtact gatttaaatc
     3121 aattgtacct tcaatttgac catcacgata catcatttca ttagtgaagt tatttatcat
     3181 cacgaaatca gcgcgatctc ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc
     3241 taaatttaaa tgtgcgtaat ccattgttac ctcccgatta caaattttgt tctatctact
     3301 atttagcaaa gtaataatac atcatatcac attaaattaa ttacttatgt cttatattta
     3361 acacttaatt tagcgattac gcaaatgaat gatttttcta aatattttat tctatcttta
     3421 tccggaaata tttgttatac gtataacttg gagaatgact acttgaaatt taatgacgta
     3481 tacaacaaac accacaaaat catacaccat cttttaaaaa aatataatat tagctataat
     3541 tatgatgagt attatcaact actcttgata aaaatgtggc aattgagtca gatatataaa
     3601 ccctcaagca atcaatcttt atcctctttt ttattcatgc gactaaactt ttaccttatc
     3661 gatctattca gacaacaaaa acgtttaaat gatatcattt tatgtgaaaa tcattcacca
     3721 atactaactg aacagccaac atactttaat gaatatgacc ttcgtttaca agatgtttgt
     3781 gccctactaa accacaggga acgtcagtgg cttatgcttt atcttgaagg ctataagcaa
     3841 tatgaaattg ctgatattat gtcattatca acttcaacaa ttaaattaat taaggcatca
     3901 gttaagcgta aatgccaacg tttttttaaa tagatgcgac gtgtcgtgtt gattaaaatg
     3961 attaatactc ttggaacgct caataatatg catttcattt atgcgaacca ttttattatt
     4021 aactgattga aaaggagtac tatatttgca agacatttct acaaaacacc tactttatat
     4081 ccaaaccgca acttcgaacc atcttgagac aaaatgtgtc tttctgtacc atgaatacaa
     4141 tctcgaattc cctattacta aagccattcc ttattatgct aaattgcatt ttacatcagc
     4201 atcaatttta atcgatgcgg caaaaagcat actcaacatt aacaagctgg tgcctattta
     4261 ccttaatccg aaaacgattc ttttcccaat aaaacaaaag cgtgcaccga tacaaacata
     4321 tatcaatgca cactatataa ttggtatgac ggaaatgggc aattcgacta tgctccattt
     4381 caaaaatggc aaaacattag aagtcgatga acccatgtct cttatttcca aaaaatgcta
     4441 tgaaagttta gcattgaagc atttcattga aaatactaat gactattaaa tttatgttct
     4501 aacagtttct ttgtaaaata tgatgttgtg atttttatta aatagatact aactaacatt
     4561 aataaaatcg ccccaatgat tgttaaaata acaaccgttg tcgacaattc tactttaggg
     4621 ttaacgaaaa attgcacacc agatagactt gtaattaatc caaataaaaa caaacctaat
     4681 aaagcaacgt atgcaaacat catcaccact aatgttacaa agatgctata acttttagtt
     4741 tctttactac gaaatacatt ttctcttaag tacgtattag caaaggtgat tggcttatta
     4801 aagttcacat tgtccttgtc agtaatacta gacaaccgtt gatctaatgc ctgtttttca
     4861 tttttgttca aaaaccataa ttgcttttta acctttttat caaatgatgc tttattcata
     4921 attaaggatt tctctccttt ataatattga cgtaatttta aacattagcg tcaattgaaa
     4981 gtgtatttta tatattttac agaaatacat gcatcaaatc tacaaaaatt aactttgcaa
     5041 ttttattttt agtaatttga cactgtataa ttgacaccgt ttatattgca aacaaatggt
     5101 tttagttaag tgtaatgatg cgcctttaaa catatctaag tttctcatat aatgaaaatg
     5161 accataccaa tgaaatgctt taatatttat tttaaagatg atagaaagga ttaaatatgc
     5221 agaaattcaa ggatttgttt tatgatgatt tatcagttgc acgtggaaat tattttctaa
     5281 ctttattatc cgcattcttg attactatta ttctactaat tggtttcgtt gtcagtgata
     5341 cacacatact ttatggcgtg tttatagtgc tcatcggtat tgtgttatta cggttattca
     5401 aaattaattt actctcattt aaaaaattaa caatgtcaca agtgatttat atcatcggtg
     5461 gtgcattgct aatttatggg ttagatcatc tttatttaca ttttcatgac gtaccgacaa
     5521 atgaacaaga attagatcga gaattacaac atatgccttt gtatatgtct attgtcacaa
     5581 ttgctattat ccctgccatt gtagaagaaa tcgtttttcg aggcatgata ataagagttg
     5641 tatttagaaa gcatttattt attggattag ttgtgtctag tctagtgttt gcatcattgc
     5701 atgaatcgga tacttggatt gggtacttac cttatttata ttctggagtg atttttggcc
     5761 taatatattt aaaaacaaag cgattagaag tcgttatttt aatccacttt ataaataacc
     5821 tatcctcatt actcattctg atatggggat gatactagtt gatataatac acaatgattg
     5881 cccctcaaca tgttattcac aaaacgatat gcaatacaaa ttaataatat actagctttt
     5941 aaaacaataa aaaaccttgt ataattaact taattttcat aaaaattaaa cgtcaaactt
     6001 tattt
//
LOCUS       DATTQF010000141         5906 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.141, whole genome shotgun sequence.
ACCESSION   DATTQF010000141 DATTQF010000000
VERSION     DATTQF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.141"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..116
                     /locus_tag="VB241_002290"
     CDS             <1..116
                     /locus_tag="VB241_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEP1245545.1"
                     /translation="PLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY"
     gene            300..1250
                     /locus_tag="VB241_002291"
     CDS             300..1250
                     /locus_tag="VB241_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1245546.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIFGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            1399..2883
                     /locus_tag="VB241_002292"
     CDS             1399..2883
                     /locus_tag="VB241_002292"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEP1245547.1"
                     /translation="MTEWTREERYQRIEDVDTDYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKCDQFNNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            2880..3839
                     /locus_tag="VB241_002293"
     CDS             2880..3839
                     /locus_tag="VB241_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEP1245548.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAHMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKSVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(4213..4929)
                     /locus_tag="VB241_002294"
     CDS             complement(4213..4929)
                     /locus_tag="VB241_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1245549.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGNEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(4948..>5906)
                     /locus_tag="VB241_002295"
     CDS             complement(4948..>5906)
                     /locus_tag="VB241_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP1245550.1"
                     /translation="VLPIFLVVYVSISYALAYILNRILKRINGTYLSLNKKFLTVITI
                     VIVITFSLLFAYSQIDASDASTIKQYSLLFLGIIILLSILIFIYSQFTLKEMKYKRNQ
                     EEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMTGLRDYFNKNIVPMK
                     DNLQMNALKLNGIENLKVREIKGLLTAKILRAQEMNIPISIEIPDEVTRINLNMIDLS
                     RSIGIILDNAIEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFST
                     KGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         2119 a          833 c         1036 g         1918 t
ORIGIN      
        1 caccattatc tgttacagaa caagaaacga atatagggtt agacaagatt gttcatggtg
       61 aacatgctta ctttgaaggt gagttaaata gattcaataa acatattcga tattagattt
      121 atatatacat agaatattca ttgtcctgac atttaactaa aggttgatgt tgggacattt
      181 tgttatacag aaattttatt ttgaaatctt tttatgaaag aagcagaaat attatttaaa
      241 gcggttacac atatgctaaa ataaggctaa gtgtcacaaa taatgatagg tgaataagta
      301 tgaaaaatat atctgatatt gccaaattgg caggcgtttc aaaaagtaca gtatctagat
      361 ttttaaataa tggatctgtc agtaaaaaaa caagtgaaaa attaacaaaa attatagcag
      421 aacatgacta tcaaccgaat caatttgctc aaagcttaag agcgagacaa acacacctta
      481 ttggtgccat tatacctaga atgaattcat atgcagtaga tgaaacaatc aaaggattgg
      541 caaaacaatg ccaaaaatat gaatcacaat taattttaaa ttatacaggt ttaaatatcg
      601 aagcagaaat acaagcgctt gaaacattag cacgcagtaa agtagatggt attgttttaa
      661 tggctacaga cataacagag agacatattg aagtcattaa taaaatgaat gtaccaatcg
      721 ttatttttgg tcaacaacat gaacaactgc atagtattgt gcatgatgat tataaagcag
      781 gtcaaattat aggtgaatgg attggtcaac agggatatca acaagttgaa gtgtttagtg
      841 tgagtgaaaa agatattgca gttggtatac atagaaaacg tggtttactt gaccagttag
      901 ccaaatatca aattaaacct aatatacatg aaacaaattt tacttatgtg gaagcacaaa
      961 aagatgttgc aaatgttttg gaaaatgtag agcaagtaga tgcggttgtt ggagcaactg
     1021 atacgattgc attagctgcc tataaatatt attctgataa aaaagatgtt atgaaaccac
     1081 atcaaatata tggttttggt ggtgatccaa tgacacaatt agtgtctcca tcgataaaaa
     1141 caattcatta taattatttt gaagctggtc aatgcgcgat ggaagagata caacagatgc
     1201 ttaaaaagca agatatgcca tatagcgtca cagtagatgt taatatttag acgctgtatt
     1261 ttttaaaata aatgtggaac cgataccata aaagtataaa tggataggtt aaaagttaaa
     1321 gaacgtaggt aaaatttgct ataatagaat ataaattgtt aacagcataa attataaaag
     1381 gaggactggg tgaatattat gaccgaatgg actagagaag aacgttatca acgaatcgag
     1441 gacgttgata ctgattattt taaaacatta aaacaacaag ttgatcaatc aaaatttcgt
     1501 caacaatttc atatacaacc agaaacgggc ttattaaacg atcccaacgg acttattttt
     1561 tataaaggaa agtattatgt ttcacatcaa tggttcccat taggcgcagt acatggctta
     1621 aagtattggt ataactacac gagtgatgac ttaataaact ttaaagctga agggccaatt
     1681 ttaaacccag ataccaaata tgacagccat ggtgtatata gcggtagcgc ttttgaatat
     1741 aacgggcatt tatattatat gtacacagga aatcatcgag ataatcattg gcaacgccat
     1801 gcgagtcaga tgatcgcacg attgaaagaa gacggttcag ttgaaaagtt tccaaagcca
     1861 gtaattagcc agcaaccaga aggatatacg agtcatttta gagatcctaa agtttttaaa
     1921 tatggtgaga aatattatgc gatcattggc gcacaaaata atgatcagca aggtcgatta
     1981 ttactttata atactgaaga tataattaat tggcattatt taggtgaaat aaatacagag
     2041 ttagatgatt ttggatatat gtgggaatgc cctgattact ttaatgtaga taatcaagat
     2101 gtcatactta tttgtccaca aggtattgaa ccaaaatgcg atcagttcaa caatatttat
     2161 caaagtggtt atatacttgg aaagtttgat attgaaaagt taacatatga acatgaaaat
     2221 tttgtcgagc ttgataatgg ttttgatttc tatgcacctc aaacattttt agatgaaaaa
     2281 ggccgacgag tactaattgg atggatgggg ttaccggaaa tcgaatatcc tacagataat
     2341 gaaggatggg cccattgctt aacaattcct cgtgtattaa atgtagaaaa tggtcaactt
     2401 aagcaacgtc catatccagc gttggaaaaa ttacgtcaca ataaagagac agcattaggc
     2461 tacgcaaata aatttactcg aaaattacat ccgtatgaag gtaaacagta tgaattaatc
     2521 atagatattt tggataatga tgctaccgaa gtatactttg aattacgtac gtctaagact
     2581 tcttcaacat taatcgctta taacaagcgt gaaaataaaa taacattaga tcgcagcgac
     2641 agtggtttat tgcctacaaa tgttgaaggt acgacgcgta gtacgatatt agaaacgcca
     2701 ttaaagcaat tacaaatttt tgttgataca tctagtatag aaattttctg taatgatggg
     2761 gaacgtgttt tgacatctcg aattttcccg acagaagacg cactaggtat taaaacatca
     2821 acagaatcag ggcaagtata tttacaattt actaaatatg atttaaagga tgaacataaa
     2881 tgagacgttt attttcaata ggagaagcgt taattgattt tattccaaat attacaaatg
     2941 cgaatttaaa agatgttcaa acatttacga agcaaattgg tggcgcgcca tgcaatgtag
     3001 cttgtacagt tcaaaagtta ggacaacaag cgcatatgat tacacaatta ggtaatgatg
     3061 catttggaga tagcattatt gaaacgatat caagtattgg tgtagatgta tcaaaagtgt
     3121 atcgcacgaa tgaagcaaac actgcgttag cttttgtgag tttgactgaa gcgggagagc
     3181 gagatttttc tttttatcgt aagccctctg ctgatatgtt atttgaacca agttttgtga
     3241 atgatattga tgtgaatgaa aacgatgtag tacatttttg ttctgttgat ttggtcgata
     3301 gtccaatgag agatgcgcat tatcaattga ttaccaaaac attaaatgca aatggtactg
     3361 tcgtatttga tcctaatgta agattgccat tatgggataa cgcagaagat ttaagacaaa
     3421 ctattcatac ctttttgcca ttggcgcata ttgtaaaagt ttcagatgaa gaattagaat
     3481 ttattacagg tattcatgat gaaaatgaag ccattcaatc attatttaca ggtaatgtca
     3541 ctgtagtcat ttatacgaaa ggagcagatg gtgctgccgt atatttgaaa aatggtatta
     3601 atcattatca tagtggctat aaggtaaaat ctgttgatac aactggggca ggggatgcct
     3661 ttattggtgc agttattagc aggattttag caaccgatgt gttaaattta actcagttat
     3721 ttgaaaatga aggtgaggaa attttagcgt ttagcaatcg cgttgctgca atagtgacaa
     3781 cgaagtatgg tgctattaat agtttgccaa cattacaaga ggttgaacaa gcattttaaa
     3841 taattcattt ataaaacagt gtaggaaaca agttgaatct tgtgctacac tgtttttttg
     3901 cgagcaaaat aagatttatt gggtaggata ttgtagcatt ttatttatgg aaactgtcga
     3961 tttcaaattt agtatatttt gttaaaaatt gcgctatagg attgtagagt gaaatagaaa
     4021 ttgataaatt atgtagtaat aggaataggt atacaatgaa acgttgtatc gttttattta
     4081 aatttacgct ttaaattaaa tttttgctat taaaaaataa aaagccagct atacagtgca
     4141 tttgctagtt atcttgttaa aatccaacaa gatttacaat tgaatacgcc gttaactgac
     4201 tttattatct tattatattt ttttaacgtt tctcaccgat gcatagcagt gttctttatt
     4261 tttaaaataa acaatacgct ctttcgaatc aatagattcg atattatgac gattgacgac
     4321 aaagctatta tgacatctga agaaacgatc atctaattga ctcagttctt ttaaattacc
     4381 ataaaattca atttgacggt tatctaaatg ggcaatgagt ctgtgagatt ttgttgatga
     4441 ttcaaaaaac ataatatcat catattgaac atacactgaa ttactgccac gttttaactc
     4501 aatcgtttca acgctattat cttttgataa taattgtaag cgtgtatgtg cagtttctaa
     4561 acaatctata attcgagttc ttaattctgc tggatcatct ttaaaaataa aatccatcgc
     4621 tgcaactttg tagacaaatg ttaaatacgt aagttcactg tgactcgtaa cgaaaataat
     4681 gttaccaact gggtcatgct tacgaatttc attgcctaat ttgataccat taatatcagt
     4741 tgaaagttga atatctaaaa agtaacagcc tatgtcattc atatttttag cttgctcaag
     4801 cacctcataa ggattatcag ttgcgagggc aatttccata ggcttttctt ctatcattat
     4861 ataatttttg ataatggtaa ccatgttttc tctttgtttt ggatcgtctt cgcaaatgaa
     4921 aattttcata cattcacatc cttatggcta gttgttaata atttcaactt tttgaatgaa
     4981 gaaaccattt tcgataattg tatctaacaa gacattgtct gcattatcag caatttcttt
     5041 taaagttgat agacctaaac cacgaccttc acctttagta gaaaaacttt cttggaacaa
     5101 ttcatgaata cgtggtatat catctgcaca tttattcata acaataaacg ttactgaatt
     5161 ttcgctttca ataaatgcaa ctcgaatgat aggatcatca atttcacttg atgcctctat
     5221 tgcgttatca agaataatac caatactgcg acttaaatcg atcatattca aattaatgcg
     5281 agttacttca tctggtattt cgatactaat cggtatattc atttcttgtg cacgtaaaat
     5341 tttagctgtt agaagacctt taatctcacg tactttaaga ttctcaatac catttaactt
     5401 aagagcattc atttgtaaat tatctttcat aggtacaata tttttattga aatagtctcg
     5461 aagaccggtc atgtcatctt cacgaatgta ttctgaaaga gtcgttaaga tattgacata
     5521 atcatgacgg aacttgcgca tttcattatt aattgcttca atctttaatg tgtattcata
     5581 ataggtttca atttcttctt gattacgttt atatttcatt tctttaagag taaattgaga
     5641 ataaataaaa attaagatac tcagtaatat aataattccc aagaataaaa gcgaatactg
     5701 tttgattgtt gatgcatcac tcgcatcaat ttgcgaatat gcaaataaca aagagaatgt
     5761 tattactata acaatagtta taacagtaag gaatttttta ttaagagaaa ggtatgtacc
     5821 atttatcctt tttaatattc tatttaaaat gtaggcaagt gcataagaaa ttgatacata
     5881 cactaccaaa aagattggta gaacta
//
LOCUS       DATTQF010000142         5737 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.142, whole genome shotgun sequence.
ACCESSION   DATTQF010000142 DATTQF010000000
VERSION     DATTQF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.142"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(139..978)
                     /locus_tag="VB241_002296"
     CDS             complement(139..978)
                     /locus_tag="VB241_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1245551.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKTVKSPVRKASQAKKVVQQPVQQASKKVVVKQAPKQTVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1747..2857)
                     /gene="prfB"
                     /locus_tag="VB241_002297"
     CDS             complement(join(1747..2784,2786..2857))
                     /gene="prfB"
                     /locus_tag="VB241_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP1245552.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDGEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(3032..3130)
                     /locus_tag="VB241_002298"
     CDS             complement(3032..3130)
                     /locus_tag="VB241_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245553.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSLKK"
     gene            complement(3172..5703)
                     /gene="secA"
                     /locus_tag="VB241_002299"
     CDS             complement(3172..5703)
                     /gene="secA"
                     /locus_tag="VB241_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP1245554.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKSVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
BASE COUNT         1685 a         1095 c          840 g         2117 t
ORIGIN      
        1 tactttcttt ttacatatca ttcaattaaa tatcatttaa atatcttctt tatataagtc
       61 tgattagatg ataccaaaaa atcctctcaa cctgttactt aaacaggtta agaggatagt
      121 cttgtcttga tatgttactt agtggatgta attatatttt cctggattta aaattgttct
      181 tgaagattta acattaaatc cagcatagtt catttccgaa acagtaattg ttccattagg
      241 atttacagat tcaacaacac caacatgtcc atatggtcca gcagctgttt ggaaaatagc
      301 gccaacttct ggtgttttat ctactttaaa tcctgcaact tttgctgcgt aattccagtt
      361 atttgcattg ccccataaac ttcctatact tctacctaat tgtgcacgac gatcgaaagc
      421 ataatatgtg cagtttccat aagcatataa gtttcctctg ttagcaactg atttattgta
      481 gttatgtgca acaggtacag ttggtactga tttttgtact tgagcaggtt tgtatgctac
      541 attaactgtc ttagctactg tttgcttagg tgcttgctta actactactt ttttagacgc
      601 ttgttgtaca ggttgttgta ctaccttttt agcttggctt gcttttctta ctggtgattt
      661 aaccgtttta gtttgtttca ctttattttg aggcacaagt gaaatcacgt caccaggaaa
      721 aattaaaggt gttacaccag gattgtatga atataattga ttcaacgtta agtgatgctc
      781 taaagcaatc ttatataatg aatcgccagc aactactgta taagttgtcg gtgattgcgt
      841 ttgtgcttga acatttgata cataattatg ttgaacaggt gtttttactt gtgtgccatg
      901 ttgttgtgca tgtgctgcat tatttaaagc taaaaaagct aacactgacg aaaccgtcac
      961 tgtaagagat tttttcatct tgctgtcatt cctttgctgt tagtatttta agtatgcaaa
     1021 tactatagca caatacattt tgtcaaaagc tattgttata acgatgtaat caaatggtta
     1081 acaatataaa aaagaatgca aactacctac tcggtgtaaa atgtattcac tgcaaatgat
     1141 tgacaacgtt ttcaacattt aattcaatac attgaaattc gttgaaggta atattactta
     1201 atgcacagta catagatttg tttaacaaac gaaactttta taaaggataa tatttcgtca
     1261 aaaaccaccg aattgtactt aaatattcat aaacaaatca tattccaaaa tctaatttac
     1321 aatttattta gctaccttta aaaaaaccaa agagcctgag acataaatca atgttcaatg
     1381 ctctacaaag ttatattggc agtagttgac tgaacgaaaa tgcgcttgta acaagctttt
     1441 ttcaattcta gtcaggggcc ccaacaaaga gaatttcgaa aagaaattct acaggcaatg
     1501 caagttgggg tgtgggcccc aacaaagaga aattggattc ccaatttcta cagacaatgc
     1561 aagttggggt gggacgacga taaagaaata ctttttctat agaaattagt atttcttatg
     1621 catgagtttt actcatgtat tcctattttt aagtacacat tagctgtggc taatgtgtaa
     1681 gaaccactac ataataaatc attagtggct ctttatcatt tctgtcccac tcccttaaaa
     1741 tatatattaa tcgtgcgaca ttgtctgtct taaatatgat tcgataaatg gtccaatgtc
     1801 tccatccatc actgcatcaa ccttacctgt ttcttcgttc gtacgatgat ctttcaccat
     1861 tgagtatgga tggaaaacat atgatctaat ttggcttccc catccgattt ctttttgttc
     1921 gccacgaatt tcagccattt cacgtgcctg ctcttccaat tttaattgat ataatttaga
     1981 ctttaacatt ttcatagctg cttcacggtt tttaatttga gaacgttcat tttggttatt
     2041 aacaactata cctgaggggt gatgggtaat tcgtattgcc gattcagttt tgttaatatg
     2101 ctgaccacct gcaccagaag ctctgaatgt atcaactgta atatcatccg gattgatttc
     2161 aatctctatt tcatcattat taaaatctgg aatcacgtca catgatgcaa atgatgtatg
     2221 acgacgtcct gatgaatcaa atggagaaat tcgtactagt cggtgtacac ctttttcagc
     2281 ttttaaataa ccataagcat tatgcccttt gatgagcaat gttacacttt taatccctgc
     2341 ttcatctcca ggtaaataat caacagtttc aactttaaaa cctttcttct cacaataacg
     2401 ttgatacatt ctaaatagca tattagccca atcttgagat tccgtgccac ctgcaccagg
     2461 atgtaactct agaattgcgt tattggcatc gtgaggtcca tctaataata attgcaattc
     2521 gtattcatcc actttagcct taaaattaat gacctcttgc tctaagtctt ctttcatttc
     2581 tccatcaaat tcttcttgta ataaatccca agtagcatcc atgtcatcta cttctgcttg
     2641 tagtgtttta taaccattaa ctattgcttt taacgcatta tttttatcta taatatcttg
     2701 cgcttttgtt tggttatccc aaaaattagg ttctgccatc atttcttcat attcttgaat
     2761 attagtttct ttgttctcta agtcaaagag accccctaat ttgtgttaaa tcttgattat
     2821 acttatctat atttcgtttg atttctgata attccatagc cttcgctcct atttataatt
     2881 caattcaagt cattgattta tatcttttat aatgctaaat tttaacataa ttttgttaaa
     2941 taacaatgtt aagaaatata agcccactga caattagttt atacttttat tgttaaaaaa
     3001 cagtacattt atcatagaca tatgcttaaa cctatttttt taaactaagt acataacaac
     3061 gtttaacaac ttcttcacat tttttaaagt atttaacgct tgtaaaataa aaagactcct
     3121 cccataacac aaactatagg tgtttaattg gaaggagtta ttttatatca tttattttcc
     3181 atggcaattt ttgaattttt taccactacc acatggacaa tcatcgttac gaccaacttg
     3241 atcgccttta acgattggtt tcggtttcac tttttcttta ccatcttcag ctgaaacgtg
     3301 cttcgcttca ccaaactcag ttgttttttc acgttcaata ttatcttcaa cttgtactac
     3361 agattttaaa atgaatttac aagtatcttc ttcgatattt tgcatcatga tatcaaataa
     3421 ttcatgacct tcattttgat agtctcgtaa tggattttgt tgtgcataag aacgtaagtg
     3481 aataccttga cgtaattgat ccattgtgtc gatatgatca gtccaatggc tatcaataga
     3541 acgaagtaaa atcatacgct caaactcatt catttgttct tctaagatat ctttttgact
     3601 ttgatatgct gcttcaatct tagcccaaac gacttcgaaa atatcttcag catctttacc
     3661 tttgatatca tcctctgtaa tgtcaccttc ttgtaagaaa atgtcattaa tgtagtcgat
     3721 gaatggttga tattcaggct cgtcatctgc tgtatttata tagtaattga tactacgttg
     3781 taacgttgaa cgtagcattg catctacaac ttgagagctg tcttcttcat caataatact
     3841 atttctttcg ttatagataa tttcacgttg tttacgtaat acttcatcgt actctaagat
     3901 acgtttacgc gcgtcgaagt tattaccttc tacacgtttt tgtgctgatt ctacagctct
     3961 tgataccatt tttgattcaa ttggtgtaga gtcatctaaa cctagtcggc tcatcatttt
     4021 ctgtaaacgt tcagaaccaa aacgaatcat taattcatct tgtaatgata aatagaagcg
     4081 actatcccct ttatcacctt gacgtccaga acgaccacgt aactggtcat caatacgacg
     4141 agattcatgt cgttctgtac ctattaccgc taaaccgcct aattcctcta cgccttcacc
     4201 taatttaata tctgtaccac gaccagccat gttagtggca atagtaacgg cacctttttg
     4261 tccagcgcct gcaacaattt cagcttcacg ttcatgattt ttcgcattta acacatcatg
     4321 acggataccg cgttttttaa gtaaatttga aatatattct gaagtctcaa ctgcaacagt
     4381 acctaatagc actggttgcc ctgccttgtg tttttctaca acatcttcta ctactgcatc
     4441 aaatttacct ttttggctaa tgtaaattaa atcagactta tcgttacgtt gcacaggttt
     4501 atttgtcgga atttgagtta ctgtcatgtt ataaatattt ctaaattctt cttcttcagt
     4561 tttagctgta cctgtcatac ccgcaagttt attgtacatt ctgaaatagt tttggaatgt
     4621 aatagacgcc atagttttag attcattttg aatttgaacg ccttccttcg cttcaatagc
     4681 ttggtgtaaa ccttccgaga aacgacgtcc tggcattgta cgtcctgtaa attgatcgac
     4741 aattaatact tcgccatcaa caaccatata gtctacatca cgttgtaatg taacgtgcgc
     4801 acgtaaagct gtgttgatat gactaataac atcaacattt tgtacatcat ataagttttc
     4861 aactttgaac atacgttcag ctttatccgc accttgttct gttaaatgta ctgattttgt
     4921 tttttcatca tatttataat cttcgtcctg ttttaacatt tttgcaaaaa catttgcttg
     4981 tgtataaagt gacgttgact tttcagcttc accagaaata attaatggcg tacgtgcctc
     5041 atcaattaaa attgagtcaa cttcatcaat gattgcgaaa tgtaatggac gcattactct
     5101 atcttcagaa taattcacca tgttatctct taaatagtca aaacctaact cattgttagt
     5161 actgtaagta atgtcttgcg catatgcttc acgtttttct tcagtcgtct tactgtttaa
     5221 gtttaatccg acagtcaaac ctaagaagtt atataactca gccatttctt cactttgaac
     5281 acttgataag tattcattga ctgtaataac gtgaacacct ctaccagcta atgcatttaa
     5341 gtatgttggc attgtcgctg ttaacgtttt accttcacct gttctcatct cagcgatatc
     5401 acctttatga attgcaatac cacccataat ttgaacttta tatggtgtca tattgaatac
     5461 acgtttagag ccttccctaa caagtgcata tgcttctggt aaaattttat ctaaataatc
     5521 attttgcttt ttgacattat caatgtcagc taattctgtt tggaattgtt tcgttttatt
     5581 acgaatttct tcatcagtta aaattgccgt tttttcttct aaagcgatta ctttatcagc
     5641 aagtttacct aactgtttaa tttctttatt attgccatca agaatttttg ataaaaatcc
     5701 catttcgttc gctcctttag ctaaaaaact gtttggc
//
LOCUS       DATTQF010000143         5717 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.143, whole genome shotgun sequence.
ACCESSION   DATTQF010000143 DATTQF010000000
VERSION     DATTQF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.143"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(144..1541)
                     /gene="sufB"
                     /locus_tag="VB241_002300"
     CDS             complement(144..1541)
                     /gene="sufB"
                     /locus_tag="VB241_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP1245555.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(1691..2155)
                     /locus_tag="VB241_002301"
     CDS             complement(1691..2155)
                     /locus_tag="VB241_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP1245556.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2145..3395)
                     /locus_tag="VB241_002302"
     CDS             complement(2145..3395)
                     /locus_tag="VB241_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP1245557.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(3501..4808)
                     /gene="sufD"
                     /locus_tag="VB241_002303"
     CDS             complement(3501..4808)
                     /gene="sufD"
                     /locus_tag="VB241_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP1245558.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEAVKRQLREVIERKVSK"
     gene            complement(4906..5667)
                     /gene="sufC"
                     /locus_tag="VB241_002304"
     CDS             complement(4906..5667)
                     /gene="sufC"
                     /locus_tag="VB241_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP1245559.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1727 a         1144 c          821 g         2025 t
ORIGIN      
        1 aagtacaaag cttatctatc aatccgattt agttataaaa caaaaaaagc cacagtaatg
       61 tggctttttg ttatattcag tatcaaaatg gtatcaataa ccgttttcga cagttaagaa
      121 tggcttaaca acgcggttta aagctaccca atactacctt ccatttcgaa cttgattaaa
      181 cggttcattt cgaccgcgta ttccattgga agttcttttg taaatggttc gatgaatccc
      241 ataacaatca tttctgtcgc ttcttcttca gaaataccac gactcattag atagaataat
      301 tgttcttcag aaacttttga aaccttcgct tcatgttcta atgatatttg atcgttgaat
      361 acttcgttat atggaattgt atctgatgtt gattcgttat ctaagattaa tgtatcacat
      421 tcaatatttg aacgagcgcc ttttgcttta cgtccaaaat gaacaatacc gcgataaata
      481 actttaccac catttttaga aatagattta gaaacaatcg ttgaagatgt atttggcgct
      541 ttatgaatca ttttagcacc ggcatcttga acttgtcctt taccagcaaa tgcaatagat
      601 aatgtactac cttttgcacc ttcacctaaa agaacacagt ttggatattt catcgttaac
      661 ttagaaccta agttaccatc tacccattcc atatttccgt tttcataaac aaaagtacgt
      721 ttcgtaacta aattgtatac attgttcgcc cagttttgaa tcgtagtata acgaacgtgc
      781 gcatctttat gcacaatgat ttccacaaca gcagagtgta aagaactagt tgtataaact
      841 ggtgcagtac aaccttctac gtaatgtaca gaagcacctt catcagcaat gattaatgta
      901 cgttcaaatt gacccatgtt ctcagagtta atacggaaat aagcttgtag tggcgtatct
      961 agtttgatat ttttaggtac ataaatgaac gaaccacctg accatactgc tgagtttaac
     1021 gccgcaaatt tgttatctgc tgcaggtact acagaagcaa agtatttttt gaataattct
     1081 tcattttctt gtaaagcact atctgtatct ttaaagataa tacctttttc ttcaagttct
     1141 ttttccatat tatggtaaac aacttcagat tcatattgag cagaaacacc agctaaatat
     1201 ttttgttcag cttcaggaat tcctaattta tcgaaagttc ttttaatttc ttcaggcact
     1261 tcatcccatg aacgttcagc ttgttctgaa ggctttacat agtaagtaat gtcatcgaaa
     1321 ttcaattctg ataagtcgcc accccattga ggcattggca ttttataaaa caattttaat
     1381 gatttaagac ggaaatctaa catccattcc ggctcatttt tcatgttaga aatttctcta
     1441 acgatattct cagttaaacc acgttctgat ctgaaaatgg acacatcatc gtcgtggaat
     1501 ccatatttat aatccccaac atcaggtgct tttttagcca tttcaatcac tccttaaaaa
     1561 tatatttaca acggcgtgcg taagttttta attcatgatg taaaccatat tataacaatg
     1621 acatgacatc atataaaaat tttttactat tatatgtcta atatcaaaat catctatgat
     1681 taacagcatt ctattcttct tcagtcgtac cttctgcttt accttcttta gcaacagtac
     1741 ctttttccaa tgctttccaa gctaatgtgg cacatttaat acgagctggg aattgagata
     1801 caccttgcaa tgcttcaata tctcccattt cttctgtaat cacatagtct tcaccaagca
     1861 tcattttcgt aaattcttgg ctcatttgca ttgcttctcc aagtgaatga cctttaacgg
     1921 cttgtgtcat catcgatgca cttgccattg aaatcgaaca accttcacct tcaaacttag
     1981 catcttttat aatgccgtct tctatatcaa atgttagtcg tatacggtca ccgcatgtcg
     2041 ggttattcat atctactgtc atagacccgt tatctaatac acctttattt ctaggatttt
     2101 tataatgatc cataatgaca gatctatata attgatctag attattaaaa ttcataagag
     2161 aaaaactcct tcgtttgttt caaggcatta attaactgat caacgtcttc tttcgtgttg
     2221 tatatataaa aactcgctct agctgttgaa gacacattta accatttcat taacggttgc
     2281 gcacaatgat gcccagctct aaccgctaca ccttctgtat ctacggctgt agcaacatcg
     2341 tgtggatgta catcttgtaa attaaacgtt attacacctg cacgacgatc ctttggcggg
     2401 ccataaattt caattccttc aattgcagac atttgctcat aagcatatat cgttaattct
     2461 tgttcatatt tatgaattgc atcaaaacct atgcgttcta aatagcgaat agcttctgca
     2521 agcccaattg cttgagcaat taatggagta cccgcctcaa atttagtagg taaatcagcc
     2581 catgttgcat catacttact tacaaaatca atcatgtcgc caccgaactc aatcggttcc
     2641 attttttgta gtaactcacg tttaccaaat aatacgccaa tacctgttgg tccaagcatt
     2701 ttatgtccac taaaactata aaaatcagca ttcatttctt gcatatcaag tttcatatgt
     2761 ggtgctgctt gcgccccatc aacactgata attgcaccat gttgatgagc tatttctgca
     2821 atggttttaa catcattaat tgtaccgagt acattagaaa tatgtgcaat agcaacgatc
     2881 tttgttttat cattaatcgt ttgcttaata tcctcgatgt ttaattcacc gtcagctgtc
     2941 attggtataa atttcaatgt cgcattttta cgctttgcta actgttgcca aggaacaata
     3001 ttggcatgat gttccatttc agtgacaaca atttcatcgc cctcttcaac atttgcatca
     3061 ccatagctat gtgctacaag gttaatcgac gcagttgttc cgcgtgtaaa aatgatttct
     3121 tcaaaatact tcgcattaat aaaacgacga acggtttcac gggcattttc ataaccatca
     3181 gttgctaatg atcctaatgt atgaacacca cgatgaacgt ttgaattata acgcttgtag
     3241 taatcttcta aaacatttaa cacttgcaca ggcgtttgac ttgtcgctgt tgaatcaaga
     3301 tatgctaaac gtttgccatt gactttttga tctaatatcg gaaaatcctt gattacttca
     3361 ttaacgtcaa atgagtgttc ggccacttca ttcacgacct ttcttaaata aaaatcctaa
     3421 tcatttaaat actgacgttg tattagtctt ataccaatat cgacagtcta tatctattac
     3481 aaacttttat tttcaaaata ttatttagaa actttgcgtt caattacttc tctcaattga
     3541 cgtttaacgg cttcgatagg taattcacgt actactggat ctaagaaacc atgtataaca
     3601 agacgttccg cttctctttg agaaatccca cgactcatta aatagtaaag ttgatctgga
     3661 tcaacacgac ctactgatgc agcatgacca gcttgtacat catcttcatc aattaataaa
     3721 ataggattcg cgtcaccacg agcatgttca gataacatta atacacgtga ttcctgatta
     3781 gcaattgatt tagttccacc atgcttaatg tagccgatac cattaaatac agatgatgca
     3841 tgttctttca taacaccatg tttaaggata taaccatcag tttctttacc atattgtacg
     3901 attttagatg ttagattaat tttttgttcg cctgtaccta caactactga tttaagtgaa
     3961 cttgttgaac gatcaccaaa taaatttgtt gtattatcaa taatttggct accctcattc
     4021 attaaaccta atgcccaatt aattgaggca tccgcttcag taataccacg tcgaatgata
     4081 tgacctgtaa agcctttatc catatagtct actgagccat atgtgatatt tgaatttgca
     4141 ccagcaatca cttcagaaat aatatttaat tgatttcctt caccagatgc atttgataag
     4201 taattttcaa catatgtgac ttcggcgctt tcttcagtaa cgatgatgac atggttataa
     4261 aagcttgcat tttcgtcgtc gtgcaacaca acgtattgta ctggatgttc tacaactaca
     4321 tttttaggaa cataaacaaa tacgccacca ttaactaatg ccgtgtgtag cgctgtgata
     4381 cgatgttcat ctactgttac tgcatctttc ataaagtact tttgtactaa atcactatgg
     4441 ttcataagag catctgctaa accttcaacg ataacgccat ctttcgatgc attatcatca
     4501 acttgtgtgt acgcaatcgt attattatgt tgaattacta agtttttaga atgatctacg
     4561 tcaataattt ctcttactga ctcaggtaat tgtgataaag attgataaac atcacctttt
     4621 acatcgtgtt gtttaaaaga atcaaaatcc cattttctta attttgtttt atcaggtttt
     4681 ggcatttcta aagtttctgt taatttcaaa gcttttttac gtaattctgt catccaagaa
     4741 ggttcattgt gggctttaga ataatcaaca agttgttctt cagaaatgtt caaaatatca
     4801 gttgtcataa ctttttcctc ctacttgatg aaatttacta tttcgtataa atgttcattt
     4861 tcatcatata tagatgaaca tctcatggat actgtattaa taagattatt cagctgaacc
     4921 gaactcttct ttaacccatt catatccttc ttcttcaaga cgttttgcta attctggacc
     4981 accagattta acgaccttac cagcatacat tacatgtact ttatcaggag taatgtaatt
     5041 taataatcgt tgatagtgtg tgatcattaa tgcaccaaag ttttccccac gcatttggtt
     5101 aataccttta gatacaactt ttaatgcatc gatgtctaac cctgaatcga tttcatctaa
     5161 gattgcaaac ttaggttcta acatcattaa ttgtaagatt tcgttacgtt tcttctctcc
     5221 acctgagaaa ccttcattta aataacgttg tgccatgtct ttatctatgt ctagaaaatc
     5281 catattttta tctaatttct taataaattg cattaagttg atttcttgtc cttcttcacg
     5341 tttcgcattg atagctgaac gcatgaaatc agcatttgta acacctgtaa tttctgatgg
     5401 atattgcatt gccaagaata atcctgcttt tgctctttca tcaacttcta attctaaaat
     5461 atttacaccg tctaaaagta cttctccttt agttacttca taacttgggt gtcccataat
     5521 tgcagatgac aaagttgatt tacctgtccc gtttggtccc ataatcgcat gtatttcatc
     5581 agtgttaatt gtcaagttaa caccttttaa gatttcttta tcctcaatag acacatgtag
     5641 gtctttgatt tctaatgttg atgccatttt atattccctc cgtaaaatat aaagttttct
     5701 taactagttt ataataa
//
LOCUS       DATTQF010000144         5711 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.144, whole genome shotgun sequence.
ACCESSION   DATTQF010000144 DATTQF010000000
VERSION     DATTQF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.144"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            50..1150
                     /gene="qoxA"
                     /locus_tag="VB241_002305"
     CDS             50..1150
                     /gene="qoxA"
                     /locus_tag="VB241_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HEP1245560.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            1150..3138
                     /gene="qoxB"
                     /locus_tag="VB241_002306"
     CDS             1150..3138
                     /gene="qoxB"
                     /locus_tag="VB241_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HEP1245561.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNGYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            3128..3733
                     /gene="qoxC"
                     /locus_tag="VB241_002307"
     CDS             3128..3733
                     /gene="qoxC"
                     /locus_tag="VB241_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HEP1245562.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            3742..4020
                     /gene="qoxD"
                     /locus_tag="VB241_002308"
     CDS             3742..4020
                     /gene="qoxD"
                     /locus_tag="VB241_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HEP1245563.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFIQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(4363..5556)
                     /gene="fmtA"
                     /locus_tag="VB241_002309"
     CDS             complement(4363..5556)
                     /gene="fmtA"
                     /locus_tag="VB241_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HEP1245564.1"
                     /translation="MKFNKEKLVIHACVLLFIIISIGLVFHRLQTKTNSIEPIHKETK
                     LSDNAKYLVDRNKGKGEPSKLKEVYNSKDPKYKKIDRYLQNSLFNGSVAVYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYIPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVRAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHSAFYDEKSFRKYFAKGYSYNSTGLSFLKP
                     NVLEQYYGAGNIYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGLFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
BASE COUNT         1760 a          985 c          928 g         2038 t
ORIGIN      
        1 ttaaaattaa tgttaagccc tacatttgta gtattaggag gtcaaaaaag tgtcaaaatt
       61 taagtctttg cttctattat ttggcacact aattttactt agtggttgtt caaatataga
      121 aattttcaac gcaaaagggc cagtagcaag tagtcagaag ttcttgattc tttattcaat
      181 cgtcttcatg cttgttattt gtttcgttgt acttggcatg ttcgccattt ttatttacaa
      241 gtatagttat aataagaatg ccgaatctgg taagatgcac cataatgcca tcattgaaac
      301 aatatggttt gtaataccta tcataatcgt tgctgcttta gctattccta cagttaaaac
      361 tttatacgat tacgaaaaac caccgaagag tgagaaagat ccaatggttg tatatgcagt
      421 aagtgcagga tacaaatggt tctttgctta tccagatgaa catatagaga ctgttaatac
      481 attaacaatc cctaaagatc gtcctgttgt atttaagctt caagctatgg atacaatgac
      541 aagtttctgg attccacaat taggtggtca aaaatatgcc atgactggca tgacaatgaa
      601 ttggacgtta gaagcatctc aaactggtac gttcagaggt cgtaattcta acttcaatgg
      661 tgaaggattc tcgcgtcaaa catttaaagt aaatgccgtt agtcaaaaag actacgacaa
      721 atgggtgaaa gaagttaaag gtaagaaaac gttagatcaa gatacatttg ataaacaatt
      781 attaccaagc acacctaata aagctttaga atttaatggt acacatatgg cgtttgttga
      841 tccagcagcc gatccagaat acatcttcta tgcatataaa cgtttcaatt tcgaattgaa
      901 agatccaaac ttcactagtg aagaaaatat gtttaaagat gtatcagaca aaccattaat
      961 acctgctcgt aaagctcaaa ttacaaatgc gaactataaa cgacatggta tgaaattgat
     1021 gattcttgga aatgacgaac catataacaa tgagttcaag aaagatgaat cgaaaaatgc
     1081 gaaagaaatg aagaaaattt caaaagatgc gcaagatcaa gacaatgatg atcatggagg
     1141 tggacattaa tgaattttcc atgggatcaa ttactagtta aaggtaactg gatgattaca
     1201 atggcacaaa ttggtgcccc attcttagtt attggtttaa tcgcagtaat tacttacttt
     1261 aaattatgga aatatcttta caaagaatgg ttcacatctg tagaccataa gaaaatcggt
     1321 attatgtatt taatctgtgc cgtattaatg ttcgtccgtg gtggtattga tgcgttactt
     1381 attcgtgcgc aactaacagt acctgataac aaatttttag aatctaacca ctataacgaa
     1441 atttttagta cacatggtgt aatcatgatt atcttcatgg caatgccatt tatctttggt
     1501 ttatggaata tcgttgttcc attacaaatt ggagcacgtg atgttgcatt cccagtatta
     1561 aacaacgtaa gtttctggct attctttgca ggtatgattt tattcaacct gtcatttatt
     1621 attggtggtt caccagcagc tggttggaca aactatgcgc cactagcagg tgaattcagt
     1681 cctggtccgg gtgttaacta ttacttaata gctatacaaa tttcagggct tggtacctta
     1741 gctacaggta tcaacttctt tgtaacaatt ttaagatgta aaacaccaac tatgaagttt
     1801 atgcaaatgc caatgtttac agtaacaaca tttattacta cattaattgt tattcttgct
     1861 ttccctcctt taacagtagc gttagcatta atgacaactg atagaatatt tgacacagca
     1921 ttcttcacag ttgcacatgg tggtatgcca atgctttggg ctaacttctt ctgggtatgg
     1981 gggcaccctg aagtttatat cgttatcctt ccagcatttg gtatttactc agaaattatt
     2041 ccgacattcg ctcgtaagcg tttattcgga catcaaagta tggtatgggc aactgccggt
     2101 atcgctttcc ttagtttctt agtttgggtt caccatttct tcacaatggg taatggtgcg
     2161 ttaatcaact cattcttctc aatttcaaca atgctgattg gtattccaac tggtgtgaaa
     2221 ttgtttaact ggttattaac attatataaa ggacgtatta catttgagtc accaatgtta
     2281 ttctcattag cattcatccc taacttcctt ttaggtggtg ttacgggtgt aatgttagca
     2341 atggcatctg ctgactatca atatcacaat acgtacttct tagtagctca cttccactat
     2401 acattggtta ctggtgttgt atttgcctgc ttagctggtt taatcttctg gtatccaaag
     2461 atgatgggtt acaaattaaa cgaaacatta aacaaatggt gcttctggtt cttcatgatc
     2521 ggatttaacg tttgtttctt accacaattc attcttggat tagatggtat gccacgtcgt
     2581 ttatacactt atatgccatc agatggttgg ttcttattaa acttaatttc aactatcggt
     2641 gcattattaa tggcaatcgg attcttattc cttgttgtta gtatcgtata cagtcacttc
     2701 aaatctccac gtgaagcaac tggagataac tgggatggcc ttggtcgtac tctagagtgg
     2761 actactgcat cagcaattcc acctaaatac aactttgcca ttacaccaga ttggaatggc
     2821 tacgacacat ttgttgatat gaaagaacat ggtcgtcatt atttagacaa ccataattac
     2881 aaagacattc atatgccaaa caatactcct gttggattct ggattggtat ctttatgact
     2941 atcggcggtt tcttcttaat tttcgaaact gtaattccag cattaatttg tttattcggt
     3001 atttttggta ctatgattta tcgcagtttc caaatcgatc acggatatca tatccctgct
     3061 gctgaagttg ctgaaactga agctagatta agagaagcgc gtattaaaga aagggaggct
     3121 gtaagtcatg agtcatgata caaacactat tgattcacgc acacatgaag gcgaattaaa
     3181 taagcttggc ttttggattt tcattacagc cgaatttgcg ttattcggta ccctatttgc
     3241 gacactatta actttgcaac atggtggcga ttatgcaggt aaaatgacta ctgagttatt
     3301 tgaattgcct cttgttttaa taatgacgtt tgcattatta ttcagttctt acacatgtgg
     3361 tattgctatt tactacatgc gccaagaaaa acaaaagtta atgatgtttt ggatgatcat
     3421 tacgttactt ttaggtttag tctttgttgg attcgaaatt tatgaattcg cacactatgc
     3481 atcagaaggc gttaacccaa caattggttc ttactggtct agtttcttta tcctattagg
     3541 aacgcatggt tgtcacgtat cgctaggtat cgtttgggcc atttgtttat taatccaaat
     3601 ccaacgacgc ggattagaca aatataatgc gccaaaatta tttatagtaa gtttatactg
     3661 gcacttctta gatgttgttt gggttttcat ctttactgcc gtatatatga tagggatggt
     3721 gtatagcgga tgagtacaat aatgaaacat actgtaggat ttatcgcatc tatcgtatta
     3781 acgcttttag cagtttacgt aacactatac acgtcattaa cattccacgc gaagttgaca
     3841 attatctttg gctttgcatt catccaagct ggacttcaat tattaatgtt catgcattta
     3901 actgaaggta aagatggacg tttacaaaca ttcaaagtta tctttgctct tgtaatcaca
     3961 ctttgtttcg ttgtcggaac atattgggtt atgcaaggcg gtcactcttc acacttataa
     4021 accacatttt aataatgtaa agtttaacca gctaacactt ttgttagctg gtttttatta
     4081 tctttcaatt tttaaatggt taagtcccct tctatatcta ataagacaat cattattatc
     4141 cattcaatta atacattctt atacaatcat ttatgtacat ttttatattg acagtcacat
     4201 caattgttta tatcttttta acttaacgca aatttatcac cctactactt caaccttatc
     4261 ctttaaactt ttcacctcta taaaaaagaa gatataactc gtatactagt caaataaggt
     4321 caatgcctta atgccatata caagttatat cttctatatt aattattgaa caataacacc
     4381 cttcgtatta taaggtttat tttgttttaa aatatcatta taaatatgtt tgattcgaac
     4441 ttcattgttt ccttttacat ttaatgcaag cacaactaca tacttatcat tatagtaaac
     4501 agtaaagact tgtccgaaga gtcccccgtt aagtctattc aatgttggct taacatagaa
     4561 accatatcga tattcatctg gatactgttt cgtaccaaac tcatgtaata atggattggt
     4621 tattttagga ctaaataatt tatattgttg tatctgagta attaatttac ccatatctgt
     4681 tggtgtcatg tatatattac ctgcaccata atattgttct aaaacatttg gcttcaagaa
     4741 agaaagtcct gtactattat aagaataacc ttttgcaaaa tattttctaa atgatttttc
     4801 atcataaaat gccgaatgct gaagttttaa tggatcacct attttagtat agtaattctc
     4861 ggcatatgat ttgccggtaa cttcttcgat aactttagct agcactaaat aatttccatc
     4921 attatacata tgctttttgt atttcttagg atcaatgcca cgtttttgaa ttgctctcac
     4981 tgcttgatct aagttcttat agtcttttga agatttatat ttatataatc cactttgatg
     5041 cagcatcaag tcttttaaag gaataggctt agatgtttta aaccatggaa tatatttgct
     5101 tactggatca ttaatattaa ttttatgttc ttcttctaac tgttttaaca gtaatcctgt
     5161 tgaaaatttt tgagctgaac caattagaaa catcgtattt ggtgtatttt taattccttt
     5221 ttcaaaatct tgatatccat agcccttact cattttcaat ttgccatttt catataccgc
     5281 tacagaacca ttaaataatg aattttgtaa ataccggtca atcttcttat actttggatc
     5341 tttactattg tatacttcct ttaatttcga tggctcacct ttgcctttat tacgatcaac
     5401 caaatatttc gcattatccg ataattttgt ttctttatgt ataggttcta tagagttcgt
     5461 tttcgtttgt aatcgatgaa aaactaatcc tatggagata atgatgaaca aaagcacgca
     5521 cgcatgtata accaatttct ctttattaaa tttcattcgc taatagcctc tcattgtatt
     5581 caaattaaat aattaaaaca ataaacttaa cttcgttata atataactta caatttcatc
     5641 tgtcacccac ttatacgtta tactacttag taatattttc ttcatataag ctcttattac
     5701 aacaaattta a
//
LOCUS       DATTQF010000145         5702 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.145, whole genome shotgun sequence.
ACCESSION   DATTQF010000145 DATTQF010000000
VERSION     DATTQF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.145"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..704
                     /locus_tag="VB241_002310"
     CDS             <1..704
                     /locus_tag="VB241_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP1245565.1"
                     /translation="KGNKKRSKTLISLVQNVVKYIVWFIVVTTILSKFGISVEGVIAS
                     AGVVGLAVGFGAQTIVKDVITGFFIIFENQFDVGDYVKINNGGTTVAEGTVKSIGLRS
                     TRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTSLR
                     SKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGIKT
                     PQPIMTPFNHSENGV"
     gene            734..937
                     /locus_tag="VB241_002311"
     CDS             734..937
                     /locus_tag="VB241_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEP1245566.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            949..2046
                     /gene="ychF"
                     /locus_tag="VB241_002312"
     CDS             949..2046
                     /gene="ychF"
                     /locus_tag="VB241_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP1245567.1"
                     /translation="MVLTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(2132..2323)
                     /locus_tag="VB241_002313"
     CDS             complement(2132..2323)
                     /locus_tag="VB241_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245568.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            2567..2863
                     /locus_tag="VB241_002314"
     CDS             2567..2863
                     /locus_tag="VB241_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP1245569.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            2884..3387
                     /gene="ssb"
                     /locus_tag="VB241_002315"
     CDS             2884..3387
                     /gene="ssb"
                     /locus_tag="VB241_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1245570.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            3439..3681
                     /gene="rpsR"
                     /locus_tag="VB241_002316"
     CDS             3439..3681
                     /gene="rpsR"
                     /locus_tag="VB241_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP1245571.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(3919..4926)
                     /locus_tag="VB241_002317"
     CDS             complement(3919..4926)
                     /locus_tag="VB241_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEP1245572.1"
                     /translation="MNRKTPKTTDGLMRHIRDNKGIQINGSTEKNQLRNIGYFHGFKG
                     YNFFLNKEEELNFEKFSELHALYSFDTEIKNLFYKHVMFCETAIKNRLLEIVCVNSGF
                     DLDSLFQKSLTYYKSYSPGSSKYKKHLKNTTRLRRDIYDMIHEHCNSKPYIYHYIHQD
                     RTVPLYAVFELFTLGNLVFFTRCMSQNLNIEAQKQLKLYSPAFDQSKDILGDIIDCMK
                     GLRNAIAHNGVLYDCRFKTGETSNRLKEYLAVKMDIHNLDFDRIIDYLILLILICSGL
                     SYSKAELQRIIRQFEQNLDFLRSKISESTYDKIIGVRARPKLRSLKNFINNLDFYLKT
                     D"
     gene            complement(4932..5596)
                     /locus_tag="VB241_002318"
                     /pseudo
     CDS             complement(4932..5596)
                     /locus_tag="VB241_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1941 a          829 c         1042 g         1890 t
ORIGIN      
        1 aaaagggtaa taaaaaacgt tcgaaaacat taatatcatt ggtgcaaaat gttgttaaat
       61 atattgtgtg gtttatagtt gtaactacca ttttaagtaa atttggcatt agtgttgaag
      121 gagtaattgc aagtgctggt gtcgtaggcc tagcagtagg ttttggtgct caaactatag
      181 ttaaagacgt aattacagga ttctttatta tatttgaaaa ccagtttgat gtaggtgatt
      241 atgttaagat aaacaacggt ggtacaactg tagcagaggg aacagttaaa tcaattggac
      301 tccgttcaac acgcattaat acaatatctg gggaacttac aatcttgcca aatagtagta
      361 tgggtgaaat aacgaattac tcaattacaa atggaacagc aattgttaaa attccagtat
      421 ctgtagatga aaatattgat aatgttgaga agaaattaaa caaattattt acatctctgc
      481 gtagtaaata ttatttattt gtaagtgatc ctgttgtgat tggtattgat gcctttgaag
      541 atactagagt aatattaagg gtttcagcag aaactatacc aggtgaaggt ttctctggtg
      601 cgcgtattat tcgtaaagaa gtacaaaaaa tgtttttaca agaaggtatt aagacaccac
      661 aaccaataat gacacctttt aatcatagtg aaaacggtgt ttagtagttt ataatacatg
      721 gaggtcatat ttaatggcgt caaaatatgg aataaatgat atagtagaaa tgaaaaaaca
      781 acatgcgtgt ggaaccaatc gttttaaaat tattagaatg ggtgctgaca ttagaattaa
      841 gtgtgaaaat tgtcaacgaa gcattatgat tccacgtcaa acgtttgata aaaaactaaa
      901 aaaaatcatc gtatctcagg atgatacaca aagataggag aatgattcat ggttttaaca
      961 gcaggtatcg ttggcttgcc aaacgttggt aaatcaacat tatttaatgc aattacaaag
     1021 gcgggtgcct tggcagcaaa ctatccgttc gcaactatag acccaaacgt gggtattgta
     1081 gaagtgccag atgcaagact acttaaatta gaagaaatgg ttcaacctaa aaaaacatta
     1141 cctactacat ttgaatttac agatattgcc ggaattgtta aaggtgcttc taaaggtgaa
     1201 ggtttaggta ataaattctt atcacatatt agagaagtag atgcaatttg tcaggttgtt
     1261 cgtgcttttg acgatgataa tgtaacacat gtagctggtc gagttgatcc aattgatgac
     1321 atcgaagtta tcaatatgga attagtactt gcagacttag aatcagttga aaaacgctta
     1381 cctagaattg aaaaattggc acgtcaaaaa gataagactg cggaaatgga agtgcgtatt
     1441 ttaacaacta ttaaagaagc tttagaaaat ggtaaacccg ctcgtagtat tgactttaat
     1501 gaagaagatc aaaaatgggt gaatcaagcg caattactga cttctaaaaa aatgctttat
     1561 atcgctaatg ttggtgaaga tgaaattggt gatgatgata atgataaagt aaaagcgatt
     1621 cgtgaatatg cagcgcaaga agactctgaa gtgattgtta ttagtgcaaa aattgaagaa
     1681 gaaattgcta cattagatga tgaagataaa gaaatgttct tagaagattt aggtatcgaa
     1741 gaaccaggat tagatcgatt aattagaaca acttatgaat tattaggatt atcaacatat
     1801 tttactgctg gtgtgcaaga agtacgtgct tggacattta aacaaggtat gactgcacct
     1861 caatgtgctg gtatcattca tactgatttt gaacgtggat ttatccgtgc cgaagtaaca
     1921 agttatgacg actatgtaca atatggtggc gaaagtggcg ctaaagaagc gggcagacaa
     1981 cgattagaag gtaaagaata tattatgcaa gatggcgata tcgttcattt cagatttaat
     2041 gtataaacga tagagtgaag ttaattaaat agtatatatg tagaagaggc ggaatcaatt
     2101 gttcgcctct tttaattatg cgtataattt attaaaagaa tggaataatt ttactcgcgt
     2161 taataatatc ttgagtgctg aaaaattgtt tgccttcgcc agtataagca ggctctaaaa
     2221 caagattagc ctttgcacaa taaagccatt caggatgaat gccactatta agtatctctt
     2281 ggaattcttg aaaatcttta gaccaatcaa tatttaaatt cattccttac cacctcacaa
     2341 tcattataga acatgtgttc gcttttgtga agtgtatttt taaaaatatc atagaaaatt
     2401 tcaaatgaat taatgtcaaa aatgatatga agagtagttt gaattattat tgtaaaaagt
     2461 aatggcgcat gatataattc tttattgtga gtaatgaaaa ttattccttg cttatctgtt
     2521 ttaagattga taagccgtat agaccacaag gaggtgcaaa tataaaatga gaacatatga
     2581 agttatgtac atcgtacgcc caaacattga ggaagatgct aaaaaagcgt tagttgaacg
     2641 tttcaacggt atcttagcta ctgaaggtgc agaagtttta gaagcaaaag actggggtaa
     2701 acgtcgccta gcttatgaaa tcaatgattt caaagatggc ttctacaaca tcgtacgtgt
     2761 taaatctgat aacaacaaag ctactgacga attccaacgt ctagctaaaa tcagtgacga
     2821 tatcattcgt tacatggtta ttcgtgaaga cgaagacaag taataattag agggggcgtt
     2881 taaatgctaa atagagttgt attagtaggt cgtttaacga aagatccgga atacagaacc
     2941 actccctcag gtgtgagtgt agcgacattc actcttgcag taaatcgtac gttcacgaat
     3001 gctcaagggg agcgcgaagc agattttatt aactgtgttg tttttagaag acaagcagat
     3061 aatgtaaata actatttatc taaaggtagt ttagctggtg tagatggtcg cttacaatcc
     3121 cgtaattatg aaaatcaaga aggtcgtcgt gtgtttgtta ctgaagttgt gtgtgatagc
     3181 gttcaattcc ttgaacctaa aaatgcacaa caaaatggtg gccaacgtca acaaaatgaa
     3241 ttccaagatt acggtcaagg attcggtggt caacaatcag gacaaaacaa ttcgtacaat
     3301 aattcatcaa acacgaaaca atctgataat ccatttgcaa atgcaaacgg accgattgat
     3361 ataagtgatg atgacttacc attctaataa aaattaacga aattaaagcg aaaaaattat
     3421 caaaggaggc acacaatcat ggcaggtgga ccaagaagag gcggacgtcg tcgtaaaaaa
     3481 gtatgctatt tcacagcaaa tggtattaca catatcgact acaaagacac tgaattatta
     3541 aaacgtttta tctcagaacg cggtaaaatt ttaccacgtc gtgtaactgg tacttcagct
     3601 aaatatcaac gtatgttgac tacagctatc aaacgttctc gtcatatggc attattacca
     3661 tatgttaaag aagaacaata atatttagtt tattgtaaaa ccccgtaagc ataggcttat
     3721 ggggttgttt ttgtgttttg ggataaaagg tgaagggcaa aaaagaggat gtgaatgttt
     3781 tgtgttcgga atttgcacaa agatatgttt atattgcaaa aataatatga atttagatgc
     3841 ataaaaaaag aggcaaccgt cagtaacagt taacctcaag tgcattccgc agatatgcac
     3901 cgctatttat gtataatatt aatctgtttt tagataaaaa tcaaggttat tgataaagtt
     3961 ctttaaactt cttaatttag gtcttgctcg aacgcctata attttatcat aagtcgactc
     4021 gcttatcttt gatcgtaaaa aatcaaggtt ttgttcgaat tgccttataa ttctttggag
     4081 ttctgcttta gaataagaga gaccagaaca aattagaatt aataaaatga gataatcaat
     4141 tattctgtcg aaatctaaat tgtgtatatc catttttact gctaaatatt cttttaatct
     4201 attacttgtc tctccagttt taaatctaca gtcataaagt acaccattgt gagctatagc
     4261 atttcttaag cctttcatac aatcaatgat gtcacctagt atatctttac tttgatcaaa
     4321 tgctggggag tataatttta gttgtttttg agcttctata tttaaatttt gggacatgca
     4381 acgagtgaaa aataccagat ttcctaaagt gaataattca aatacggcat atagtggcac
     4441 ggttctatct tgatgtatgt aatgataaat gtaaggtttt gaattacaat gttcatggat
     4501 catgtcgtaa atatctcttc ttagtctagt agtatttttt aaatgttttt tatattttga
     4561 actaccggga ctataacttt tataatatgt taatgatttt tgaaataaac tatctaaatc
     4621 aaagccagag tttacacaaa ctatttctag caatctattt ttaatagccg tttcacagaa
     4681 cataacgtgt ttatagaaaa ggtttttaat ttctgtatca aaactgtata aagcgtgtaa
     4741 ttcgctgaat ttttcaaaat taagttcttc ttctttgttt aaaaagaaat tatacccttt
     4801 gaatccatga aaatagccga tgtttcgtaa ctggtttttt tccgtgctac catttatttg
     4861 gataccttta ttgtctctta tatgtctcat taaaccatca gtagttttag gtgtttttct
     4921 gttcatagtt tttaactccc caatcgttca aatttattca gcatatcttt cgccatctga
     4981 ttagtaacat gtgtgtatat ctctagattt ttttataatc tgaatgacct acatgctctt
     5041 gcattgcttt taagttaatt cctaattgag caagtgtaga tatatgcgaa tgatgtaatg
     5101 tatgcgtcgt tgcaggtttc ttaatagaac taatctcagt tgtctctttg atagcccccc
     5161 ctttaataat gttatttatt ttgtttaaat cgataggacc accagctgca tttgtgaata
     5221 tgtaccctat attaataaac ttatcattca aagcataagt gttcttagta agtcgatgct
     5281 atggatagtg aggcctatag tcctatagct gttacttgtt ctagttgtct cttttactcc
     5341 ggatgcttcc atttttaatc agttatccaa ttaattgtac catcgatatc tagcgtttta
     5401 tcttcatagt ttatattttc tctttttatt gcaagtagct caccgatacg catgccatta
     5461 gcaatttgaa actgcactat agattttacc tttttataat tacgttttct cgttgggtat
     5521 ttttatgctg aattagatag tcgaaacaat ccggtatttc tttcactaca ttatctttta
     5581 aattgttatt acatatagtt agtaacgcat cactataggg atatctattt tatctattac
     5641 acatataact ttgaattgct tactatttta aattaacaaa ttttattctc ttagaatttg
     5701 tc
//
LOCUS       DATTQF010000146         5538 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.146, whole genome shotgun sequence.
ACCESSION   DATTQF010000146 DATTQF010000000
VERSION     DATTQF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.146"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            81..869
                     /locus_tag="VB241_002319"
     CDS             81..869
                     /locus_tag="VB241_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245573.1"
                     /translation="MSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTL
                     DMYPIKNLEDLYDKEGYRDGEFEKGDKGMWVLYSSIVSEFKGESLKSRGMILKLDRNK
                     RTAKGSYIIRELKEDKNHDVQKNEKKYPVKLVNNKIIPTEDVKNEDLKREIENFKLFS
                     QYGEFKSLNTDRITNISYNPNAPNYSAEYKINDDDNNIKQLKNRFNIKSNKNPKLLFK
                     GAGNIKGSSVGYKEIQIIFNRNKEESVSCIDSIEFKPSEGDYNE"
     gene            882..2189
                     /locus_tag="VB241_002320"
     CDS             882..2189
                     /locus_tag="VB241_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245574.1"
                     /translation="MRCWQIKKDTEPTSAISFMAYEIENANNHNLSKMAIKEQLDRSA
                     ERGMFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKLKEGEFS
                     RQNVTNALETVKDGYADIKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGG
                     RDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDA
                     LTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLKGYAEENNKSF
                     VKHSNNAISKLASIELLRANMMTANGGGLSSSQQKVLDSLTALTIAQSFSQLIDDEIN
                     QIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNEVLNALDEGQVNESSMVREPEQMI
                     SAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2186..2509
                     /locus_tag="VB241_002321"
     CDS             2186..2509
                     /locus_tag="VB241_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245575.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2528..2842
                     /locus_tag="VB241_002322"
     CDS             2528..2842
                     /locus_tag="VB241_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245576.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3131..3391)
                     /locus_tag="VB241_002323"
     CDS             complement(3131..3391)
                     /locus_tag="VB241_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245577.1"
                     /translation="MSTLLYNRDKDISHERESRCINMLFVILVLYIIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3510..4712
                     /locus_tag="VB241_002324"
     CDS             3510..4712
                     /locus_tag="VB241_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEP1245578.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEGAITKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5371..5433)
                     /locus_tag="VB241_002325"
     CDS             complement(5371..5433)
                     /locus_tag="VB241_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HEP1245579.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
BASE COUNT         2157 a          770 c         1016 g         1595 t
ORIGIN      
        1 tatgaaatat tcaaatgtgt ataaaaataa agcagagaga tgtgaaatga tggagtatat
       61 aaaaaaaaat tgctttgtac atgagtgtat tacttttaat catttttatt gggggatgtg
      121 gaaatatgaa agatgaacag aaaaaagagg aacaaacaaa taaaacagat tcaaaagaag
      181 aacaaatcaa aaagagtttt gcgaaaacgt tagatatgta tccaattaag aatctcgagg
      241 atttatacga caaagaaggc tatcgagatg gcgaatttga aaaaggtgac aaagggatgt
      301 gggttctgta ttcatctatt gtatcagaat tcaaaggcga gagtttaaaa tcacgaggaa
      361 tgatattaaa gttagataga aataagagaa ctgctaaagg aagttatatt ataagagaat
      421 tgaaagaaga taaaaatcat gatgttcaaa aaaacgaaaa aaagtatcca gttaaattgg
      481 tgaataataa aataattcca actgaagatg taaaaaacga agatttaaaa agagaaattg
      541 aaaacttcaa attattttct caatatggtg aatttaagag tttaaatacg gataggataa
      601 caaatatttc ttataatcct aatgcgccta actattcagc tgaatataaa attaatgatg
      661 atgataataa catcaaacaa ttaaaaaatc gattcaatat taagtctaat aaaaatccta
      721 agttattgtt caaaggagca ggtaatataa aagggtcttc tgttggatat aaggagatac
      781 agataatttt caataggaac aaagaagaaa gtgtttcttg tattgatagt atagagttta
      841 aaccgagtga aggagactat aatgagtaag agtgtctata aattagatgt tggcaaatta
      901 aaaaagatac tgaaccgaca agcgcaatat catttatggc gtatgaaatt gaaaatgcta
      961 ataatcataa tttgagtaag atggcaataa aggaacagtt agatagaagc gcagaaaggg
     1021 gcatgttccc ttctaatctt gaatatgttg atagttacac tgattctctt actggagtaa
     1081 caacttctgc ttttttaaat aaagatacag gcaaagtaac tcttgggatg actgggacta
     1141 atatacaagg cgaagccttt aaaaagttaa aagaaggtga attttcaaga caaaatgtta
     1201 ccaatgcttt ggaaacagtt aaagatggat atgcagatat taaaatatta tattctcctg
     1261 catctgatca aaactataga tatgcgaata cacaagaatt tataaataaa ataaaaagta
     1321 agtatgacat tgattttatt actggacatt cactaggtgg aagagatgcg gtagttctag
     1381 gaatgagtaa tggtattccg aacattgtgg tttataatcc agctcctatt tctataacta
     1441 gtttgaatcc taattcccca gatggaaaac gattgttaga attatataaa aattataaag
     1501 gtaatattac taggtttgtt gcagaaaatg atgcattgac agaaaatctg aagaaatata
     1561 agcattatgt ttttttcggt aatgataaag tctttaaaaa tggtaaaggt catgaaatgg
     1621 aaggctttct gaccgaagaa gaacaaaaag ctataaaaaa agaacttaaa aaactaaaag
     1681 gttatgcaga agaaaataat aagtcatttg taaagcattc aaataatgct atctctaaat
     1741 tagctagtat agaattactt agagccaata tgatgactgc aaatggagga ggattatctt
     1801 cttcgcagca gaaagtttta gacagtttaa cagctttaac aattgcgcag tcattcagtc
     1861 aactgataga cgatgaaatt aatcaaatca aaaaaatgta taatgaaaag aaaaagaaat
     1921 ttggaaaaaa ttgggaagac gctcaaaaag ctggaaaagc tgtaggtgaa gatttgagtg
     1981 taaatgaagt tcttaatgct ttagatgaag gtcaagtgaa tgaaagtagt atggtaagag
     2041 aacctgaaca aatgatatct gcaaaagaaa gacaactttc aacgataggg tcttctgtat
     2101 caaattatat tatgagagtt agactcagta ttaatgaaat cgtagataaa gatcaagtgc
     2161 ttgcatcaca aataggtgga ttactatgat gtttaatcaa attaataata aaaatgaatt
     2221 agaagaatca tatgaatccg agaaaaaacg tatagagaat gaactgcaaa atttaaatga
     2281 acttaggcat agaactcgaa aagaaaatga acgtagttat gatgtttttc aatatttgaa
     2341 gcacgaaatg aattatagtg aagatgccca aaggaaaatg acgagaaata tagaagcgta
     2401 tgagcaagaa atcaatgaga taattagaaa gcaagaatgg aaattagaag aatataaaga
     2461 agacttaaaa aagtcttatg aaaagcagtt agataaacta agtgactgat aatttggagg
     2521 agattaaatg actctaatag aaccagatat gaccttaaga atgccagata taagtactac
     2581 agtagaaaca cttaatctca tatctaaaat gaatgctcaa aaagaaaata ttcgcacagt
     2641 tattgcacct gaacataagc ataaatacaa agatattgaa aacggattaa aaggtgaaga
     2701 aaaagtatta attgaacaaa tggcgcaaca ttgcgaggct tttaaagcta attttaaagg
     2761 cgcagctcaa ggagattggg ttaaaagtgc catgtctgag atagacagca ttaaggatga
     2821 cctgaaaaaa attaatagct aaactacata tcattatact aaataaagcg accaatgttc
     2881 agtgtattca caactgacac taggccgctt ctttttaatt tattttattt ccatgtaaag
     2941 taacgctctc ttaatcgaaa tatcacactc acaattgtaa aaacacaact taaattactg
     3001 tcccgcatta aacatatcca cattccaaac agcaacaaca aaatcctcaa gtaccaaaag
     3061 ctatgaccac tttatcaaaa tcaacgcaag ttaacctctc ctgaactacc cgccataaat
     3121 catcttcaac ttacaaacca taaagcgctg cagttaagta ttgaatggca tagccaattg
     3181 aaataaaaat agcaatcgtt ataatagcac tgccaatggt atataaagta tgtttcgtag
     3241 ataatccttc agtctttgaa ccaaaaacac tgagtagaat aaatgcaata ccaatgatat
     3301 ataaaactaa aatgacaaat aacatgttta tacacctcga ttctctctcg tgagatatat
     3361 ctttgtctct attataaagt aaagtgctca ataatttaat taagtatact atttttttat
     3421 tatcgaagat tgcgaaaggt gaaatgctcc tatataatgt aaaacggaat tattcctatt
     3481 tacaaataaa ttgaagtgag ggtatatata tggctaaaat tccagttacg gtattaagtg
     3541 gttatttagg ctcggggaaa acaacgttgt taaatcatat tttacaaaat cgtgaaggtc
     3601 gacgtatcgc agtaattgta aatgatatga gtgaagttaa tatcgataaa gatcttgtcg
     3661 cagatggtgg gggactatcg cgcacagacg aaaaattagt cgaactttct aacggttgta
     3721 tctgttgtac acttagagac gatttattaa aagaagttga gcgtttagtg aaaaaaggtg
     3781 gcatcgatca aattgttatt gagtcaacag ggatttcaga gccagtacct gttgcacaaa
     3841 ctttctcata tattgatgat gaacttggca ttgatcttac agcgatttgc cgtttagata
     3901 caatggttac agttgtggat gctaaccgct tcgtccatga catcaactca gaagatttat
     3961 tgatggatcg tgatcaaagc gttgatgaaa cagatgagcg ttcgattgct gatttattaa
     4021 ttgaccaagt tgaattttgt gatgtattga ttattaataa aattgactta ataagtgaag
     4081 aagaactagc gaagttagaa aaagtgttaa gtgcattgca accgactgct aaaattatta
     4141 agacaacaaa ttctgaagta gatttaaaag aagtcttgaa tacgcagcgt tttgattttg
     4201 aaaaagcgag cgagtcagca ggatggatca aagaacttga gtctggtggg catgcatcgc
     4261 atacacctga aacagaagaa tatggtatat catcgtttgt atataaacgt cgtctacctt
     4321 tccatgctaa aaggttcaat gattggttag aaagcatgcc aaataatgtc gttcgatcaa
     4381 aaggtatcgt atggttagca caatacaatc atgtagcatg tttattatct caagcagggt
     4441 catcttgcaa tattcatcca gttacatatt gggtggctag tatgtctgaa gcgcaacaaa
     4501 cgcaaatatt agcagaacgc caagacgtcg cagctgaatg ggatccagaa tatggcgatc
     4561 gtcatacaca atttgtcatt attggtacag acttagatga aggtgcaatt acaaaagaac
     4621 tcgatgcatg cttagtcaat gcgcaagaaa ttgatgcaga ttggcaacaa tttgaagatc
     4681 catatcaatg gcaaattaga ccggcacgat aagttgaatg aagtgtaatc atttttaaat
     4741 tgtggcttaa ttgtttgcaa tttatgacaa ttaataaaaa gttaagatgt tcttaggatc
     4801 tttttgtttt caatctatct aaagtgtgat agttttgata aagcagaaat ttgcatttta
     4861 tccatagggc taggacatgt atgtgtctta gtccttttta tatttgcgtt gatgaaaaat
     4921 gacaaaacca tcgtaccaac tgttaattga aagtacaatc attgtttaaa ggtcataaaa
     4981 atgagtgaag caaatgcagc tatcaatgta ctattactgc ttacagtcac acgagtgttt
     5041 cagcctaaac gtgcaatgaa attctgcacg cgtccaatat atcatgcgct agatcatgaa
     5101 gatcattaag atttcacatg ccaaatcatg aatgtctcag acctcgagta tcaaacataa
     5161 aacacgccac aaaaatataa cgcgtcagac tacaaagatc acgaatattc cgtacgtccc
     5221 actaagaaca tcctatacaa aaaacaccaa gaccatttat ttgcaacaaa ctacccagac
     5281 tcatctcaca tcaataaatc aacacaaagc aaagccacca tccctattgg tatagtggcc
     5341 tgagaatttt aagtattcaa ttcgcttaaa ttattttgcg aaaatgtcga taattgcttt
     5401 gatgatttta atgatagtac ctacaatagc catcgttttg tcctcctgta tgttgattta
     5461 acatagtgat gagttgttga tcgttaatgt ttgagattag tagttaccta aaaatttacc
     5521 aagtaaatct ttaaagaa
//
LOCUS       DATTQF010000147         5526 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.147, whole genome shotgun sequence.
ACCESSION   DATTQF010000147 DATTQF010000000
VERSION     DATTQF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.147"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            250..1368
                     /gene="ald"
                     /locus_tag="VB241_002326"
     CDS             250..1368
                     /gene="ald"
                     /locus_tag="VB241_002326"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEP1245580.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLATSHDLDYKEILNVIE"
     gene            1463..2503
                     /gene="tdcB"
                     /locus_tag="VB241_002327"
     CDS             1463..2503
                     /gene="tdcB"
                     /locus_tag="VB241_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HEP1245581.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2534..3856
                     /locus_tag="VB241_002328"
     CDS             2534..3856
                     /locus_tag="VB241_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP1245582.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            4013..5404
                     /gene="norB"
                     /locus_tag="VB241_002329"
     CDS             4013..5404
                     /gene="norB"
                     /locus_tag="VB241_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HEP1245583.1"
                     /translation="MEKPSRETFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWCWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITILAIAIVSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLKAGSLSITYLVMVLIMIRVGEKLLQTFGCKKP
                     MLIGTAVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIDTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1943 a          775 c         1035 g         1773 t
ORIGIN      
        1 tctaaaaaac atatttgatt aattattttt tgagtcaatg tattaataca actataaatc
       61 aaatggagta taagaaataa atatagtggt ataattgcac ataatattta agaataataa
      121 taatatatta aatatttatt aacaaattat agataaaata tgaataatta attaatggta
      181 tttacatatt cataaccaaa ggataaaata attgtgaaat tattcacaaa tgaaagagga
      241 gtgttgcaaa tgttagttgc agtagtcaaa gaattaaaac aaggtgaagg acgtgtagct
      301 tgcacacccg aaaatgtgcg taagttaacg gatgcaggac ataaagtaat tgttgaaaaa
      361 aatgctggca ttggttcagg attttctaac gatatgtatg aaaaagaagg cgctaagatc
      421 gtaactcacg aacaagcatg ggaagctgat cttgttatca aagtaaaaga acctcatgaa
      481 agcgaatatc aatatttcaa aaagaatcaa attatctggg gatttttaca tctagcatct
      541 tcaaaagaaa tagtagaaaa aatgcaagaa gttggtgtaa ctgcgattag tggtgaaacc
      601 attataaaaa atggaaaagc agaattatta gcgccaatga gtgctatagc aggtcaacgc
      661 tcagcaatta tgggagctta ctactctgaa gcacaacatg gtggtcaagg tactttagtg
      721 actggtgtac atgaaaatgt ggatatacct ggtagtacat atgtgatttt cggtggtggc
      781 gtagcagcaa caaatgcagc aaatgttgcc ttgggactaa atgctaaagt aatcattatc
      841 gagttaaacg atgaccgcat taaatatctt gaagatatgt atgcagaaaa agatgtcaca
      901 gtagtcaaat caacaccaga aaatttagca gaacaaatta aaaaagcaga tgtatttatt
      961 tctacaattt taattcctgg tgcgaaaccg ccaaaattgg ttactcgtga gatggttaaa
     1021 tcaatgaaaa aaggttcagt attaatcgat atagctattg accaaggtgg aactattgaa
     1081 acaattagac caactacaat ttctgatccg gtgtatgaag aagaaggtgt gattcattat
     1141 ggtgtaccaa atcaaccagg agcagtccca agaacttcaa caatggcatt agcacaagga
     1201 aatattgatt atatattaga aatttgtgac aaaggcctag aacaagcaat taaagataat
     1261 gaagccttaa gtactggagt aaacatttac caaggacaag tgacaaacca aggattagct
     1321 acatcacatg acctagatta taaagaaata ttaaatgtta tcgaatagat agtgatttaa
     1381 atgaaattga gtgaaatgaa tattttaaat atagcattat agtttggact aaaaatttac
     1441 aaaacggaag gatgtaatta ttatgacaac caacacagtt acattacaaa cagcacatat
     1501 tgtaagttta ggagatatcg aagaagctaa agcaagcatt aaaccattta ttcgtcgaac
     1561 acctctaatt aaatcaatgt atttaagcca aagtataact aaagggaatg tatttctaaa
     1621 attagaaaat atgcaattca caggatcttt taaatttaga ggcgctagca ataaaattaa
     1681 tcacttaacg gatgaacaaa aagaaaaagg cattatcgca gcatctgctg ggaaccatgc
     1741 tcaaggtgtt gctttaacag ctaaattatt aggcattgat gcaactatcg taatgcctga
     1801 aacagcacca caagcgaaac aacaagcaac gaaaggctat ggggcaaagg ttattttaaa
     1861 aggtaaaaac tttaacgaaa ctagacttta tatggaagaa ttagcgaaag aaaatggcat
     1921 gacaatcgtt catccatatg acgataagtt tgtaatggca ggccaaggaa caattggttt
     1981 agaaatttta gatgatattt ggaatgtgaa tacagtcatc gtaccagttg gcggtggagg
     2041 attaattgca ggtattgcca ccgcattaaa atcatttaac ccttcaattc atattatcgg
     2101 tgttcaatct gagaatgttc atggtatggc tgagtctttc tataagagag atttaactga
     2161 acatcgagtg gatagcacaa tagcagatgg ttgtgatgta aaagttcctg gtgaacaaac
     2221 atatgaagta gttaaacatt tagtagatga atttattctt gttactgaag aagaaattga
     2281 acatgctatg aaagatttaa tgcagcgtgc caaaattatt actgaaggtg caggcgcatt
     2341 accaacagct gcaattttaa gtggaaaaat aaacaataaa tggcttgaag ataaaaatgt
     2401 tgttgcttta gtttcaggcg ggaatgttga cttaactaga gtttcaggtg tcattgaaca
     2461 tggactgaat attgcagata caagcaaggg tgtggtaggt taaaacattt aatctgaaaa
     2521 atgaggtgta attatgtcaa atggtaaaga attacaaaaa aatataggtt tcttctcagc
     2581 gtttgctatt gttatgggga cagttattgg ttcaggagta ttctttaaaa tatcaaacgt
     2641 aacagaagta acaggaacag caggaatggc cttgtttgta tggttcctag gcggcatcat
     2701 taccatttgt gcggggttaa cagcagcaga acttgctgct gcaatccctg aaacaggtgg
     2761 cttaacaaag tatatagaat atacatacgg tgatttctgg ggcttcctat caggctgggc
     2821 acaatcattt atttattttc cggcaaacgt agcagcattg tctatcgtat ttgcgacaca
     2881 gttaattaat ttattccatt tatctatagg ttcattaata ccaatagcaa tcgcatctgc
     2941 gttatctatt gtgttgataa atttcctagg ttcaaaagca ggcggaattt tacaatcagt
     3001 taccttagtt attaaattaa ttccaattat tgttatcgtt atctttggta ttttccaatc
     3061 aggagacatc actttttcat taattccaac tacaggtaat tcaggaaatg gtttctttac
     3121 agcaattggt agtggtttat tagcaactat gtttgcatat gatggttgga ttcatgtagg
     3181 aaatgttgcg ggagaactta aaaatcctaa acgcgattta cctttagcga tttcagttgg
     3241 tatcggttgt attatggctg tgtatttatt aattaacgct acattcttat taacactgcc
     3301 aatagaacta cttgcaggaa atttaaatgc agcatcagat acatcaaaaa tattatttgg
     3361 agaaaatggc ggtaagatta tcacaatcgg tatattaata tctgtttatg gtacgatcaa
     3421 tggctatact atgactggta tgcgcgtacc atatgcaatg gctgaaagaa aattattgcc
     3481 atttagccac ttattcgcaa aattaacaaa atctggtgca ccatggtttg gcgcaattat
     3541 acaacttata atcgctatca tcatgatgtc aatgggtgcc tttgatacaa ttacaaacat
     3601 gttaattttt gttatttggt tgttctattg tatgtcattt gttgcggtaa taattttaag
     3661 aaaacgtgaa ccaaatatgg aacgaccata taaagtaccg ttatatccaa tcataccttt
     3721 aattgctatt ttggcaggat catttgtatt aattaataca ctgtttacac aatttatatt
     3781 ggcaattatt gggattctaa taacagcact tggtatacca gtttattact ataagaagaa
     3841 acaaaaagca gcataaggta agataactag cattgagaat aattgaatgg actactaata
     3901 aatttaaagt tttgcacatt aaaatcaaaa accattcaat tattctatcg aacagacaaa
     3961 tttctgttat ggaatttgtc tgtttttcaa aagtataggg aggcaaatag agatggaaaa
     4021 gccgtcaaga gagacatttg aaggcaataa taagttgtta ataggaattg ttttaagtgt
     4081 aataacgttt tggctatttg cacaatcatt ggttaatgtt gtaccaatac ttgaagatag
     4141 ttttaataca gatattggaa cggttaatat cgctgttagt ataactgctt tattttcagg
     4201 aatgtttgta gtaggagcag gtggtcttgc tgataaatat ggcagaatta aactcacgaa
     4261 cattggtatt atcttaaata tattgggttc attattaatc attatttcaa atattccttt
     4321 attacttatt ataggaagat taattcaagg actttcagca gcatgtatta tgcctgcaac
     4381 tttgtctatt attaagtcat attacattgg gaaagataga caacgcgctt taagttattg
     4441 gtcaattggc tcatggggcg gctctggtgt ttgttcattt tttggaggtg cagttgcaac
     4501 acttttaggt tggtgttgga ttttcatctt atcaattata atttcattaa ttgcgctgtt
     4561 tcttattaaa ggcacacctg aaactaaatc taaatcgatt tctctaaata aatttgacat
     4621 taaaggtctg gttcttttag tcattatgct tctcagttta aatattttaa ttactaaagg
     4681 atcagaatta ggtgttacct cacttctttt tatcactata ttagcaattg caattgtatc
     4741 ttttagttta tttatagttc ttgaaaagcg tgctacaaat cctttaatcg attttaaatt
     4801 atttaaaaat aaagcttata caggtgcaac agcttcaaac tttttgttaa atggtgttgc
     4861 aggaacatta atagtagcca acacatttgt tcaaagaggt ttaggatatt cttccttgaa
     4921 agcaggaagt ttatcaatca cttatttagt aatggtacta attatgattc gtgttggtga
     4981 aaagttactt caaacattcg gatgcaagaa accaatgcta attggaacag cagttcttat
     5041 tgtcggagaa tgtctcattt cattaacatt tttgccagaa atattatatg tcatttgttg
     5101 tattataggt tatttattct ttggtttagg actaggaata tatgctacac catcaacaga
     5161 tacagcaatt gcaaatgcac cgttagaaaa agtaggcgta gctgcaggta tctataaaat
     5221 ggcttctgca ttaggaggag catttggcgt cgcattgagt ggtgcagttt atgcaatcgt
     5281 atcaaatatg acaaacattg atactggtgc aatgattgca ttatggttaa acgcaggtat
     5341 gggaatttta tcattcgtta tcattttgtt acttgtgcct aaacaaaacg acactcaatt
     5401 atgataattg agaattaaat tgaaatcata aaggtcgcta caatattaaa caaaaatata
     5461 aaccgattct tatgtgtaat cattataaat gattctacat aaggtcggtt ttttattacc
     5521 ctttta
//
LOCUS       DATTQF010000148         5499 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.148, whole genome shotgun sequence.
ACCESSION   DATTQF010000148 DATTQF010000000
VERSION     DATTQF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.148"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            268..732
                     /gene="accB"
                     /locus_tag="VB241_002330"
     CDS             268..732
                     /gene="accB"
                     /locus_tag="VB241_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP1245584.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPFESAAMPMPQVQSTDNNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            732..2087
                     /gene="accC"
                     /locus_tag="VB241_002331"
     CDS             732..2087
                     /gene="accC"
                     /locus_tag="VB241_002331"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP1245585.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2102..2464
                     /locus_tag="VB241_002332"
     CDS             2102..2464
                     /locus_tag="VB241_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP1245586.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2524..2913
                     /gene="nusB"
                     /locus_tag="VB241_002333"
     CDS             2524..2913
                     /gene="nusB"
                     /locus_tag="VB241_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP1245587.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPNLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2930..4267
                     /locus_tag="VB241_002334"
     CDS             2930..4267
                     /locus_tag="VB241_002334"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP1245588.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4260..4490
                     /locus_tag="VB241_002335"
     CDS             4260..4490
                     /locus_tag="VB241_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP1245589.1"
                     /translation="MTKETQSFEEMMQELERIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4468..5349
                     /locus_tag="VB241_002336"
     CDS             4468..5349
                     /locus_tag="VB241_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP1245590.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMNTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELMQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         2101 a          761 c         1038 g         1599 t
ORIGIN      
        1 acatatcata ttgttgagga taagaaataa tagctaataa aaataacatt gatatgttta
       61 actgaatgca taaaattttt aatcaagagt taagatgagt caatgttaag taataaatcc
      121 acaagtgttt ttaatttgaa atgtggtttt attataactt tgggatagac ctataatgtc
      181 gcctgcgata tgaacatgct tgaattgaag agttgtctca agtaaaatag acgggtagat
      241 gaaaacaaac tgaaggagtc agtaataatg aactttaaag aaatcaaaga attaattgaa
      301 attctggata aatcaacttt aacggaaatc aatattgaag atactaaagg caaagtgacg
      361 cttaagaaag aaaaagaaac tgagattatc acgccacaaa tctcacaaat gccatttgaa
      421 tctgcggcaa tgcctatgcc tcaagtacaa tcaactgata acaataaaac tgaagctcca
      481 aagccaactt cagataatca caaaacaatt aatgcaccta tggtaggtac attttacaaa
      541 tcgccatctc cagacgaaga agcctatgtg caagttgggg acactgtttc aaatgaaaca
      601 acagtgtgta ttttagaagc aatgaaacta tttaatgaaa ttcaagcaga aatttcaggt
      661 gaaattgttg agatcttagt agaagacgga caaatggtag agtatggcca accgttattt
      721 aaggtgaaat aatgaaaaag gttttaattg caaaccgcgg tgaaatcgca gttaggatta
      781 ttcgcgcttg tcgtgattta ggcatccaaa ctgttgcaat ctattctgaa ggggataaag
      841 atgcgctaca tactcaaatt gctgatgaag catattgcgt aggtcccact ttgtctaaag
      901 attcatattt aaatattccg aacatcttat ctattgcaac ttctacaggt tgtgatggcg
      961 ttcatccggg ttatggcttt ttagctgaaa atgctgactt tgcagaatta tgcgaagcat
     1021 gccaattgaa gttcattgga ccaagttatc aatctatcca aaaaatgggt atcaaagatg
     1081 ttgctaaggc agaaatgatc aaagccaatg ttccagttgt tcctggtagt gacggtttaa
     1141 tgaaagacgt ctcagaagct aagaaaatcg ctaaaaaaat tggttatccg gtcatcatta
     1201 aagctactgc tggtggtggc ggaaaaggta tccgtgttgc tcgtgatgaa aaagaacttg
     1261 aaactggctt ccgaatgaca gaacaagaag cacaaactgc atttggtaat ggtggacttt
     1321 atatggagaa attcatcgaa aacttccgcc atattgaaat ccaaattgtt ggggacagct
     1381 atggtaatgt aattcattta ggagaacgtg attgtacaat tcaaagacgt atgcagaaat
     1441 tagtggaaga agcaccttcc ccaattttag atgatgaaac acgtcgtgaa atgggaaatg
     1501 cagcagttcg tgcagcgaaa gctgtaaatt atgaaaatgc gggaacaatt gagtttatat
     1561 atgatttaaa tgataataaa ttttatttta tggaaatgaa tacacgcatt caagtagaac
     1621 atcctgtaac tgaaatggta acaggaattg atttagttaa attacaatta caagttgcta
     1681 tgggtgacgt gttaccgtat aaacaagaag atattaaatt aacaggacac gcaattgaat
     1741 ttagaattaa tgctgaaaat ccttacaaga actttatgcc atcaccaggt aaaattgagc
     1801 aatatcttgc accaggtgga tatggtgttc gaatagagtc agcatgttat actaattata
     1861 cgataccgcc atattatgat tcgatggtag cgaaattaat catacatgaa ccgacacgag
     1921 atgaagcgat tatggctggc attcgtgcat taagtgaatt tgtggttctt ggtattgata
     1981 caactattcc attccatatt aaattattga ataacgatat atttagaagc ggtaaattta
     2041 atacaaactt tttagagcaa aatagcatta tgaatgatga aggttaatag gaggtcaatc
     2101 catggtcaaa gtaactgatt attcaaattc aaaattaggt aaagtagaaa tagcgccaga
     2161 agtgctatct gttattgcaa gtatagctac ttcggaagtc gaaggcatca ctggccattt
     2221 tgctgaatta aaagaaacaa atttagaaaa agttagtcgt aaaaatttaa gccgtgattt
     2281 aaaaatcgag agtaaagaag atggcatata tatagatgta tattgtgcat taaaacatgg
     2341 tgttaatatt tcaaaaactg caaacaaaat tcaaacgtca atttttaatt caatttctaa
     2401 tatgacagcg atagaaccta agcaaattaa tattcacatt acacaaatcg ttattgaaaa
     2461 gtaatgtcat acctaattca gtaattaaat aaagaaaaat acaaacgttt gaaggagtta
     2521 aaaatgagtc gtaaagaatc ccgagtgcaa gcttttcaaa ctttatttca attagaaatg
     2581 aaggacagtg atttaacgat aaatgaagcg ataagcttta ttaaagacga taatccagat
     2641 ttagacttcg aatttattca ttggctagtt tctggcgtta aagatcacga acctgtatta
     2701 gacgagacaa ttagtcctaa tttaaaagat tggactattg cacgtttatt aaaaacggat
     2761 cgtattattt taagaatggc aacatatgaa atattacaca gtgatacacc tgctaaagtc
     2821 gtaatgaatg aagcagttga attaacaaaa caattcagtg atgatgatca ttataaattt
     2881 ataaatggtg tattgagtaa tataaaaaaa taaaattgag tgatgttata tgtcagatta
     2941 tttaagtgtt tcagctttaa cgaaatatat taaatataaa tttgatcaag acccacatct
     3001 ccaatctgta ttgattaaag gtgagctttc aaatttcaaa aagcattcaa gtggtcattt
     3061 atactttaat gtgaaagata aagaaagcgt tataagtgcc atgatgttca aaggtagtgc
     3121 ttctaaatta aacttcgaac ctaaagaagg agatgaagtc ttattagaag cacgtgtttc
     3181 tgtttttgaa cgtcgtggaa actatcaaat ttatgtaaat aaaatgcaat tagatggtat
     3241 agggaactta tatcaaaaat tagaagcatt aaagaaaaaa ttaactgaag aaggttgttt
     3301 tgataaagcg aataagaaat caatacctaa gtttcctaaa aaaattgcag ttttaacagc
     3361 gagtacaggt gccgcaattc gagatatcca ttcaacgata aatagtcgtt ttccattagc
     3421 tgaacaaata caaattagta ctttggttca aggtgaaaaa gcaaaagacg acattattga
     3481 aaaaattgaa tatgcagata gtttaggtgt agataccatt attgtaggtc gaggcggtgg
     3541 atccatagaa gatttatgga atttcaacga agaagctgtc gtccgtgcaa tatataattg
     3601 taagacaccg attatatcag cagtaggtca tgaaacagac tttacattaa gtgattttgc
     3661 tgcagacatc agagctgcga ctccaactca agctgctgtt attgcaacac cagaccaata
     3721 tgaattgctg caacaaatac agcaatatca gtttacattg actcgtttca tcaagaaaca
     3781 tttagaacaa caacgtaaac atgttgaaca tttgtcatcg tattacaagt ttaaacaacc
     3841 aactttattg tatgatcaac agatacaacg tcgtgatgat ttagaaaaga gactgaaaca
     3901 acaaattcag gcaacttttg aacaacagag acatcgatta atgctgttgc agcaacgcta
     3961 taatttaaaa gcgttactaa gtagtgttaa tcaagaacaa caaaataatc ttcaattgac
     4021 aaaccaactt gttaaattat tgaattctaa gatattaagt tataaaaatg atttgaaaaa
     4081 taaagtagaa aacttaaaca atttaagtcc aactaataca atgttgcgtg gatatgcaat
     4141 tgttaataaa aaagacgaag tcattacgag tactaaagat ttaacagaaa atgatcaatt
     4201 gacgttaaca atgaaagatg gcttagtaga tgcaaaagtt acgaaagtaa ggtgtaataa
     4261 tgactaaaga aacgcaaagt tttgaagaaa tgatgcaaga attagagcga attgttcaaa
     4321 aattagataa tgaaacagta tctttagagg aatcattaga tttataccaa cgtggtatga
     4381 aactatcagc agcttgtgac acaactttaa aaaatgccga aaaaaaggtg aatgacttaa
     4441 taaaagaaga agctgaggat gtaaaaaatg acgaatctac cgatgaataa attaatagat
     4501 gaagtcaata atgaattatc ggttgcgata aataaatcag taatgaatac tcagctagaa
     4561 gaaagtatgt tgtattcatt aaatgctgga ggtaaacgca tccgaccagt tctgttatta
     4621 ctcactttag attcactaaa taccgagtat gagttaggta tgaagagcgc aattgcacta
     4681 gaaatgattc atacatattc acttattcat gatgacctac cagcgatgga taatgatgat
     4741 tatcgacgag ggaaattaac gaatcataaa gtatatggtg agtggacggc gatattagca
     4801 ggtgatgctt tattaactaa agcatttgaa cttatttcaa gtgatgatag attaactgat
     4861 gaagtaaaaa taaaagttct acaacggctg tcaatagcaa gtggtcatgt tggaatggtc
     4921 ggcggtcaaa tgttagatat gcaaagcgaa ggccaaccaa ttgatcttga aactttggaa
     4981 atgatacaca aaacaaaaac aggagcacta ttaacttttg cggttatgag tgcagcagat
     5041 atcgctaatg tcgatgatgc aactaaagaa catttagaaa gttatagtta tcatttaggt
     5101 atgatgtttc agattaaaga tgatttatta gactgctatg gtgatgaagc gaagttaggt
     5161 aaaaaagtgg gcagcgatct tgaaaataat aaaagtacgt acgtgagttt attaggaaaa
     5221 gatggcgcag aagataaatt gacttatcat agagacgcag cagtggatga actaatgcaa
     5281 attgatgaac aattcaatac aaaacactta ttagaaatcg ttgatttatt ttatagcagg
     5341 gatcactaaa agcaaatata tcgattagca acaattgtta agtttgaaat gtctcaaaag
     5401 gcatattcaa ctgacgttgt ttgttaatcg atttttctca tgttgaaact taaaattatg
     5461 ttaaagactt tcatctaaaa atagaatttt caatttcta
//
LOCUS       DATTQF010000149         5496 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.149, whole genome shotgun sequence.
ACCESSION   DATTQF010000149 DATTQF010000000
VERSION     DATTQF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.149"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            271..1146
                     /locus_tag="VB241_002337"
     CDS             271..1146
                     /locus_tag="VB241_002337"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP1245591.1"
                     /translation="MFIIELIKGIILGIVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            1318..2001
                     /locus_tag="VB241_002338"
     CDS             1318..2001
                     /locus_tag="VB241_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245592.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEVKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            2004..2462
                     /locus_tag="VB241_002339"
     CDS             2004..2462
                     /locus_tag="VB241_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEP1245593.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVITQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEKSLLRVIHRIKELD"
     gene            2464..3030
                     /locus_tag="VB241_002340"
     CDS             2464..3030
                     /locus_tag="VB241_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEP1245594.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(3125..3667)
                     /locus_tag="VB241_002341"
     CDS             complement(3125..3667)
                     /locus_tag="VB241_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245595.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(3747..4046)
                     /locus_tag="VB241_002342"
     CDS             complement(3747..4046)
                     /locus_tag="VB241_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245596.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            complement(4184..4573)
                     /locus_tag="VB241_002343"
     CDS             complement(4184..4573)
                     /locus_tag="VB241_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245597.1"
                     /translation="MKKLIISIMAIMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKEASQDKQSKDQ
                     TKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            complement(4640..5083)
                     /locus_tag="VB241_002344"
     CDS             complement(4640..5083)
                     /locus_tag="VB241_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245598.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            5210..>5496
                     /locus_tag="VB241_002345"
     CDS             5210..>5496
                     /locus_tag="VB241_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245599.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKY"
BASE COUNT         1862 a          883 c          831 g         1920 t
ORIGIN      
        1 tgagtaccgg aaaaatttta tattgaaaca gtattgttgt taattttttc acaattatat
       61 cacctaaacc tattataaaa ggttttcctt aaaatcaaat taaatagctc aataaaatat
      121 tgtagtttta catgtaaaag aaaatgaata tatacatctt tagtctagta ttataactat
      181 aactagcatg tctttaaatg aatatgctat catgatatgg taattttgat atttaaataa
      241 attaaattga gtaatgaaag tgggtacatt atgtttatca ttgaattaat aaaaggtatt
      301 atcttaggga tcgtcgaagg attaacggaa tttgcccctg tttcctctac tggacatatg
      361 attctagttg atgatatgtg gttaaaatca tctgaatttt taggttctca atcagcattt
      421 acatttaaaa tcgtcatcca attaggatcc gtctttgcag cagcatgggt gttccgcgaa
      481 cgcttcttag agattttaca tattggtaaa cacaaacatg ttgaaggaga taacaatcaa
      541 caaagacgtt caaagccaag acgtttaaat ttattacatg tattagtggg tatggtgcca
      601 gcaggtattt taggcttact atttgatgat ttcatcgaag aacatttatt tagtgtgcca
      661 actgttatga ttggcttatt cgtaggtgct atctatatga ttattgctga taaatactca
      721 gctaaagtta aaaatccaca aacagtagat caaatcagtt atttccaagc atttgtcatc
      781 ggtatttctc aagctgtagc tatgtggcct ggtttcagtc gttcaggttc aacgatttca
      841 actggggtat taatgaaatt aaatcacaaa gctgcatcag actttacatt tattatggct
      901 gttccaatta tgttagcggc tagtggttta tctttattaa aacattatca agatattcag
      961 attgctgata taccttttta tattttaggc tttttagctg catttactgt tggactaatt
     1021 gcaatcaaaa cattcttgca tcttattaac aaaatcaaat taattccatt tgccatttac
     1081 agaattgtat tagtaatctt tattgctata ctttacttcg gatttggcat tggtaaaggt
     1141 atttaatttc cttttaaaat atagactgtc aattaaggaa ccacttgatt ggcagttttt
     1201 attgtcttct ttgaaaaaac atctgaaata cttataagtg attcactact ctcatcgttt
     1261 atattgatgt atcatagggt atgtaaaatt aataaagtaa ttatggaggc aagatctatg
     1321 gataagaaaa aagtcatcaa atttatgatt aatgtattac caattgtatt ggtaccgtta
     1381 attgttgaac gtaaacgtat caaacaacat ccggacgtac aaaaagttac agatgctaca
     1441 agtaaagttg cttcaaaaac atctgcagca atcagtaaca cagcgagtga tgttaaagaa
     1501 tatgtcggcg ataaaaaaca agattttgaa aataagcgtg aacttaaaaa gtttgctaga
     1561 gaacatgatc ctgcctatat tgagaaaaaa ggcgaaaaat tagctaaaca aaatcgtaaa
     1621 gacgctgata aaatgaataa aatacttcaa aaaaatatcg aaaagcgtca taaagaagag
     1681 caaaaagccc gcgaaaagaa tgaaatacaa cgtattaaag atatgaaaaa gtcacaaaaa
     1741 tacgaagtaa aagcaggctt aacacctaat aaattagatg agaaaactga gaaaaaaggc
     1801 gataaactag ctgaaaaaaa tcgcaaagaa atcgctaaaa tgaataaaaa gttacaaaaa
     1861 aatattgaaa aacgacacaa agaagaacaa aaacgccaac aagaagctga taaagcacgc
     1921 atcaagtcat ttaaaaaata taaagattat gttgccaaaa gcgcctctca acaaaataaa
     1981 gaaaacaata cagaggcata attgtgacac atattattat tgatggagat gcttgtcctg
     2041 ttgttgattc tattatagat ttaacaactg agacaggcat ttttgtgaca attattcgga
     2101 gcttcagcca tttttcgaac caattatatc ccccacatgt atcaacatta tatgttgatg
     2161 atggaccaga tgcagttgat tacaaaattg ttcaattatc aacgaaagat gatatcgtaa
     2221 tcactcaaga ttatggactc gcaagtttat tagtcgacaa agtcttaatt gtcatgcatc
     2281 ataatgggaa gatatacaat tccaaaaata ttcaacaact attagataaa cgatatatga
     2341 atgcacaaat tagaaaacaa ggtggtcgcc acaaaggtcc cccacctttt acaaagcaag
     2401 atcaaaaagt gtttgaaaaa tcactattaa gagtcattca tcgaattaag gagctggatt
     2461 aatatgaagc gaatcgcagt ttattgtggt gcaagcaaag gtcatgaccc ttcatatgta
     2521 caaaaagcat acgatttagg taaatatttt gctgaacaag gttatgaatt agtatttggt
     2581 gcagggtcaa ttggtattat gggtgccatt caagatggtg tattagatca tggtggcaaa
     2641 gctattggcg tcatgcctaa aatgttagat gaacatgaaa tcacaagcca acgtttaact
     2701 gaattaattt tagttgattc tatgcatgaa cgtaaaaata aaatggcaga acttgcagat
     2761 gcatttgtca tggctcctgg tggcgcaggt tcactagaag aatttttcga aatgtatagt
     2821 tgggctcaaa taggtatcca tgaaaagcct attgccattt ataatattaa tggctttttc
     2881 aatccactac aaacaatgat tgaccatatg attgaggaag gttttattga tcctaagtat
     2941 cgtgcacttg caccgttatg cgacactaag gaatcattaa tagaatcaat attaaatttc
     3001 aaacctctag gtactcgttc atacgattaa tcattctaag gttaaaaaat aaacaaacac
     3061 ccctaatatt gttatcatca gctaaacaat attaggggtg tttgttacgc ttatgtataa
     3121 attttcactt tcgaagatta atgtaatagc gcacttgcct ttttaataaa tgatacttac
     3181 ttagattggg taattcgaaa tgatcataca ctgtcatgcc aattttttcc attacacgtt
     3241 gtgaagcttt atttgcttct gctgtaaaac tatagacatc gtatatttga tgttgttctg
     3301 ctaacttcaa cgttgccttt gcgccttcag ttgctaatcc ttttccccaa aattctggca
     3361 acaagcgcca acctatttca taaagcggta attctttaaa tggataatcg cttgtttctg
     3421 gaatataatt caaacctata aagcctatcc attgatggga ctctttatct tctacagcaa
     3481 ataatccaat gccataatct ttaataaccg catccatagt tctcatatct aattctgaac
     3541 gacgataact caataaactt ggaaaatatt tacgtacgtc ataattcgca ttcatttttt
     3601 gaaaaggtaa cagatcatct tcatgccagt ctcttaatat taaacgctct gtttcacaat
     3661 aaatcatcga ttgacactcc tatataattt ctcaataaaa aacacaaaat cgatattttg
     3721 atcaactttg cgttttagat agcagtttat tttgatttag aaatatagct tataagttca
     3781 acatgaactg gcacttcttt taaacgtgct ctatctgatt tcagcgcagt aaaagttgca
     3841 tctataaaat cttgttgatg gtttaaacga tatgtagcaa caaaagtatc tgtatcggac
     3901 tcaaagtttg gtaaatgtgc aattacacgc ttaatttcgc cagtagaatc agcaatcgtg
     3961 cgtcctgtaa tattttctaa ttctctacct aattctggca aagtaatatc ttgaccctct
     4021 aaaattctaa aatcttgttc atgcatgccg tttctctcct ttattaatta cgttgtctat
     4081 ttttacgttc tgtttcaatc atgaccacat tttaattaat acataccctt aaaataaggt
     4141 ttaaaacgct gtacctttca tctataacaa gtaagcaatt caattatgat tggtgtttgt
     4201 cattagcttt ttgatcttta tctgttgatg taggcttttt gtgattatct ttatccgatg
     4261 acttagtttg atcttttgat tgcttatctt gtgatgcctc tttatctttg tctttatttt
     4321 tgtcttcgtc ttttttatcg tccttgttat cttcttttga aggttttaaa tctttcactt
     4381 ctttttcaag gtctttttgc ttatctgcta atttttcttt ttcttgttga agcttttctt
     4441 ttttatcttt taattcttta ttttcttttt gtaaattatc gatatccttt tccagagtgg
     4501 ctttctcttg gcttttacca caacctgtta aaaatagcat gatcgccata atgctgatga
     4561 ttaatttctt catataaaac tccttaacgt cattaaatct tttttaattt taacatgaat
     4621 tagcaacata atcctaattc taatcaatat ctttatacat ttttacatga ctcacttgat
     4681 aacctaaatc tttattcaaa gatatcattg gtaaattact tttatgaatt gtactgctta
     4741 ttcgctttgc attcatagtt tttgcccatt tttcaagcgc aatcttcagt tgcgtagcta
     4801 ttcccagttt gcgaaattgt ggttcaacat atagcagttc aatgttaacc atactttttt
     4861 cattactaaa atgtccccaa ataaacgcta ttaattgacc ttcattttca tatatataaa
     4921 ttttatcatt tgtatgttct aatcttgaac atatcatctc gtacctaaga gcaatactca
     4981 gtttagtaca tttatagttg gattctgctt gcgacaataa tgtctcatgt atattagcta
     5041 tttgcttgat ataattatgt tcatctttat taagtgtcct cattttcttc tccgatatta
     5101 atcgttatat ttatgcgttt aatatgatat gattgacctt attaatacta taaaatcatt
     5161 tccattttag tttggaatta aacattttta aaggagcgct ttagtaacaa tgaaaagtac
     5221 tgcgcaatta acaaaagaaa ataatgttaa atcattaaga ttaagcaata ctgatagaga
     5281 aatcttcgag aattatatga cttatatgcg ctctgacttt cgcgtcaatc ctcatgatac
     5341 agagttaatt attaaccgta tacttaaaca attattaagc gcagaacaac atggtttatt
     5401 agccttagat tttttcaatc acgacccaaa agcacatgca ataaaagaac taaaagcgat
     5461 gcctaatgaa acatttaaaa atatttttaa atatat
//
LOCUS       DATTQF010000150         5445 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.150, whole genome shotgun sequence.
ACCESSION   DATTQF010000150 DATTQF010000000
VERSION     DATTQF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.150"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1163
                     /locus_tag="VB241_002346"
     CDS             <1..1163
                     /locus_tag="VB241_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245600.1"
                     /translation="SSSSSLGLATHTVEAKDNLNGEKPTTNLNHNVTSPSVNSEMNNN
                     ETGTPHESNQAGNEGTGSNSRDANPDSNNVKPDSNNQNPSPDSKPDPNNPNPGPNPKP
                     DPDKPKPNPEPKPDPDKPKPNPDPKPDPDKPKPNPDPKPDPNPNPNPKPDPNKPNPNP
                     SPNPNQPGDSNQSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFL
                     ALANGAYKYNPYILNQINQLGKEYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFR
                     FQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTV
                     VKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            complement(1188..1931)
                     /locus_tag="VB241_002347"
     CDS             complement(1188..1931)
                     /locus_tag="VB241_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1245601.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     YTQIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            2106..2390
                     /gene="groES"
                     /locus_tag="VB241_002348"
     CDS             2106..2390
                     /gene="groES"
                     /locus_tag="VB241_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP1245602.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            2466..4082
                     /gene="groL"
                     /locus_tag="VB241_002349"
     CDS             2466..4082
                     /gene="groL"
                     /locus_tag="VB241_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP1245603.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDATIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEVGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(4151..5323)
                     /locus_tag="VB241_002350"
     CDS             complement(4151..5323)
                     /locus_tag="VB241_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP1245604.1"
                     /translation="MASYEKRGNTWRYRISLGKDAETGKYKYISNSGFKRKSDAKHHA
                     EMVERQLRNGDYIAPSTSTFKQVADDWISQYANEVKVSSVRAREKAINHAIERFNNKP
                     IQTINKHEYQRFVNDISAQYSKNYVDSIIASTNMIFKYAYDMKLIRIMPSEGIKRPKK
                     KISVEELEDTEIHKKFLEKDELFQFLEVAKNHHSPQNSFEVFCTLAYTGMRAGELLAL
                     KWSDIDFENNTINITKTYYNPNNNKKQFQILTPKTESSIGKISVDPHVIKLLKNYKTD
                     VQDTWKNELYVDNNFVFTDVNGYPLVIKKLQLWIKAILKKTDITNKQISTHSFRHTHC
                     ALLIEAGVHIKEIQERLRHKDINTTMNIYAKITNSYKKDASQKFSKLMENVSKDLF"
     gene            complement(5337..>5445)
                     /locus_tag="VB241_002351"
     CDS             complement(5337..>5445)
                     /locus_tag="VB241_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP1245605.1"
                     /translation="DIEELFPNKGEKSTTLHKEILQTDSRLSSDIFKEY"
BASE COUNT         1880 a          866 c         1026 g         1673 t
ORIGIN      
        1 gtagtagtag tagtagttta gggcttgcaa cgcacacagt tgaagcaaag gataacttaa
       61 atggagaaaa gccaacgact aatttgaatc ataatgtaac ttcaccatca gtaaatagtg
      121 aaatgaataa taatgagact gggacacctc acgaatcaaa tcaagctggt aatgaaggaa
      181 ctggttcgaa tagtcgtgat gctaatcctg attcgaataa tgtgaagcca gactcaaaca
      241 accaaaaccc aagtccagat tcaaaacctg acccaaataa cccaaaccca ggtccgaatc
      301 cgaagccaga cccagataag ccgaaaccaa atccggaacc aaagccagac ccagataagc
      361 cgaaaccaaa tccggatcca aaaccagatc cagacaaacc gaagccaaat ccggatccaa
      421 aaccagatcc aaatccgaat ccgaatccaa aaccagaccc taataagcca aatccaaatc
      481 cgtctccaaa tcccaatcaa cctggggatt ccaatcaatc tggtggctcg aaaaatgggg
      541 ggacatggaa cccaaatgct tcagatggat ctaatcaagg tcaatggcaa ccaaatggaa
      601 atcaaggaaa ctcacaaaat cctactggta atgattttgt atcccaacga tttttagcct
      661 tggcgaatgg ggcttacaag tataatccgt atattttaaa tcaaattaat caattgggga
      721 aagaatatgg tgaggtaact gatgaagata tctacaatat catccgtaaa caaaacttca
      781 gcggaaatgc atatttaaat ggattacaac agcaatcgaa ttactttaga ttccaatatt
      841 tcaatccatt gaaatcagaa aggtactatc gtaatttaga tgaacaagta ctcgcattaa
      901 ttactggcga aattggatca atgccagatt tgaaaaagcc cgaagataag ccggattcaa
      961 aacaacgttc atttgagcct catgaaaaag atgattttac agttgtaaaa aaacaagaag
     1021 ataataagaa aagtgcgtca actgcatata gtaaaagttg gctagcaatt gtatgttcta
     1081 tgatggtggt attttcaatc atgctattct tatttgtaaa gcgaaataaa aagaaaaata
     1141 aaaacgaatc acagcgacga taatccgtgt gtgattcgtt ttttatatta tggaataaaa
     1201 atgtgatata taaaattcgc ttgttccgtg gcttttttca aagcttcagg attaagtaat
     1261 tggaatataa cgacaaatcc attttgtaac atatgaataa taattggaac agcaagtcgt
     1321 ttcgtccata catatgctaa tgaaaaaatg acgcccatac caaagtatat aggaataaat
     1381 ttatagtcat tatgcgctaa tgcaaagatt aatgagctta cagtcgttgc aatagcaaat
     1441 gcgacgatac gattaccttt aattgcatta aataattcgc caaagattac ttttctaaat
     1501 acatattctt ctaatagagg accaacgatt gatacgaaaa agataaacaa tggaatctta
     1561 cgtgcaataa cgattaaacg ctcagtattt ggacttgttt gttgtgtgcc aaaaatttga
     1621 gtatatacaa tactaactac catttgataa atcattacca gtgcaaatcc aagcaatgcc
     1681 catggaatga tatatttttt aggttcttta acttctaatt ctaattttgt tggattttta
     1741 atttttaaat taattaaaat aatcgtcgtg gcggcgatta aaaatagaac aagttgtatg
     1801 taaatgactg ctttagtcag ttctatgcca ctatattgta caaatggtaa tttttttaca
     1861 atgagaagcg gtaaaaattg agacaatata taaataataa cagttagcaa tgatgcccat
     1921 agtcttgtca taattttcct ccaaatattt gtttataatt tattttatcg taaataactt
     1981 gaagttacaa aacttaattg aaaggttatg acttgaagtt ttgaccaaat ttgattatta
     2041 taaatgtatg ttagcactct ataaagttaa gtgctaaact ttaggttttt taaggaggaa
     2101 caatcatgct aaaaccaatt ggaaatcgtg tgattattga gaaaaaagaa caagaacaaa
     2161 caactaaaag tggtattgtt ttaactgata gtgctaaaga aaaatcaaac gaaggcgtta
     2221 tcgttgcagt aggaacagga cgcctattaa atgatggtac aagagtgact cctgaagtga
     2281 aagaagggga ccgtgtcgtg ttccaacaat atgcaggtac agaagttaaa cgagataatg
     2341 agacttattt agttttaaat gaagaagata ttttagcggt aattgaataa tatagaatat
     2401 aattcataga tagattgtaa agaacgaaaa tgaaatatga ctaaacaaat ggaggtttat
     2461 cacttatggt taaacaattg aaattctctg aagatgcacg tcaagcaatg ttacgtggtg
     2521 ttgaccaatt agcaaatgca gttaaagtaa cgattggtcc taaaggacgt aatgttgtat
     2581 tagataaaga gtttacagca cctttaatta cgaacgatgg tgtaacgatt gctaaagaaa
     2641 tcgaattaga agatccatat gaaaatatgg gggctaaact agttcaagaa gtcgcaaata
     2701 agacaaatga aattgctggt gacggtacga caactgcaac agtattagct caagcaatga
     2761 ttcaagaagg cttgaaaaat gttacaagtg gtgcaaaccc agttggttta cgacaaggta
     2821 tcgacaaagc agttaaagtt gctgttgaag cgttacatga aaattctcaa aaagttgaaa
     2881 ataaaaatga aattgcgcaa gtaggtgcga tttcagcagc agatgaagaa attggacgct
     2941 atatttcaga agctatggaa aaagtaggta acgatggtgt cattacaatt gaagaatcaa
     3001 atggcttgaa cactgaactt gaagtggttg aaggtatgca atttgatcgt ggttatcaat
     3061 caccgtatat ggttactgat tcagataaaa tggttgctga attagaacgc ccatatattt
     3121 tagtaacaga taaaaaaatc tcttctttcc aagatatctt acctttatta gaacaagtgg
     3181 ttcaatctaa tcgtccaatc ttaattgtag ctgatgaagt tgaaggcgat gcattaacaa
     3241 atatcgtgct aaaccgtatg cgtggcacat ttacagctgt tgcagtaaaa gcacctggtt
     3301 tcggtgatcg tcgtaaagca atgttagaag atttagctat tttaactggt gcgcaagtga
     3361 ttacagatga tttaggctta gaccttaaag atgcaacgat tgatatgtta ggtactgcaa
     3421 gtaaagtaga agttactaaa gataatacaa ctgttgtaga tggtgacggt gacgaaaaca
     3481 gcattgatgc acgtgtaagc caattgaaat ctcaaattga agaaactgaa tctgactttg
     3541 atcgtgaaaa attacaagaa cgcttagcta aattagcagg cggtgttgca gttatcaagg
     3601 taggtgcagc aagtgaaaca gaacttaaag aacgtaaatt acgtatcgaa gatgctttaa
     3661 actctacacg tgcagcagtt gaagaaggta ttgttgcagg tggtggtact gcactagtaa
     3721 atgtttacca aaaagtaagt gaaattgaag ctgaaggcga cattgaaaca ggtgtgaata
     3781 ttgtacttaa agcattaact gcaccagttc gtcaaattgc tgaaaatgca ggattagaag
     3841 gctctgtcat cgttgaacgc ttgaaaaatg cagaaccagg tgttggtttt aatgctgcta
     3901 caaacgagtg ggttaatatg ttagaagtag gtatcgttga tccaactaaa gtaacacgct
     3961 cagcattaca acatgcagca agtgttgcgg caatgttctt aacaactgaa gcggttgtgg
     4021 catcaattcc agaaaaaaat aatgaccaac ctaacatggg tggcatgcca ggaatgatgt
     4081 aaaatgactg ttaaacgccg attttataac gtttgtaata ttgggtggtc ataatttggt
     4141 catagaaatt ttaaaataaa tcttttgaga cgttttccat gagtttacta aacttttgag
     4201 aagcgtcttt tttgtatgag ttcgtaatct tagcgtagat gttcatagtg gtatttatat
     4261 ctttatggcg caagcgttct tgtatttcct taatatgcac accagcctct ataagtaacg
     4321 cacaatgagt atgacgaaat gaatgagtgc ttatttgttt attagttatg tcagtctttt
     4381 taagtatagc ttttatccat aattgtagtt ttttaattac aagagggtag ccgttaacat
     4441 cagtaaaaac gaaattatta tctacataca attcgttttt ccatgtgtcc tgcacgtcgg
     4501 ttttataatt tttaagtaat ttaatcacat gaggatccac tgaaattttt ccgattgagc
     4561 tttcagtttt tggtgtaagt atttgaaatt gctttttatt gttattcgga ttgtaataag
     4621 tctttgtaat attgattgtg ttattctcaa agtctatatc agaccatttc aatgccaata
     4681 attcacctgc acgcatgcct gtatatgcta atgtacaaaa cacctcaaag ctgttttggg
     4741 gtgaatggtg atttttagca acctccagga attgaaataa ttcatctttt tcaagaaact
     4801 ttttatgtat ctcagtatct tctaattctt ccacactaat tttcttttta ggtcgtttaa
     4861 taccctcgct aggcattatt cttattaatt tcatatcgta tgcgtactta aatatcatat
     4921 ttgtagaggc tataatgcta tcaacataat tcttgctata ctgtgcgctt atatcgttta
     4981 caaaacgttg atattcatgt ttattgatag tttgtattgg tttattgtta aagcgttcta
     5041 tggcgtggtt tatggctttc tcgcgtgctc tgacactact tacttttact tcgttagcat
     5101 attgtgatat ccaatcgtca gcaacctgtt taaatgtaga tgtggacggt gcgatataat
     5161 cgccatttct taactgacgc tcaaccattt cagcgtgatg tttagcgtct gatttgcgtt
     5221 taaaacctga gtttgaaata tatttatatt tgcccgtttc tgcgtctttt cctagtgata
     5281 tacgatagcg ccatgtattt ccgcgttttt cataacttgc catttgatca cctcgattaa
     5341 tattctttaa aaatatcact agataaacgg ctatcagttt gtaaaatttc tttatgtaga
     5401 gtcgtacttt tttcgccttt atttggaaaa agctcttcaa tatcg
//
LOCUS       DATTQF010000151         5412 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.151, whole genome shotgun sequence.
ACCESSION   DATTQF010000151 DATTQF010000000
VERSION     DATTQF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.151"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            176..634
                     /locus_tag="VB241_002352"
     CDS             176..634
                     /locus_tag="VB241_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245606.1"
                     /translation="MADRTNKEIKTGRFIATASIAFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            701..934
                     /gene="secG"
                     /locus_tag="VB241_002353"
     CDS             701..934
                     /gene="secG"
                     /locus_tag="VB241_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP1245607.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            1063..1803
                     /locus_tag="VB241_002354"
     CDS             1063..1803
                     /locus_tag="VB241_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEP1245608.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDKEMHHFKPTET
                     LKELSEALDTIKDQVDEVLDPILVIQAENDNMIDPQSANYIYEHVDSDEKDIKWYSQS
                     GHVITIDKEKEQVFDDIYQFLESLDWSE"
     gene            1837..4209
                     /gene="rnr"
                     /locus_tag="VB241_002355"
     CDS             1837..4209
                     /gene="rnr"
                     /locus_tag="VB241_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP1245609.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERSKTDRYQKKHSSKGQSKLIKGTLSQNKKGFAFLRPKDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLEEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGHTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIESERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGKQRKGKNQRKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            4231..4695
                     /gene="smpB"
                     /locus_tag="VB241_002356"
     CDS             4231..4695
                     /gene="smpB"
                     /locus_tag="VB241_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP1245610.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            4786..5147
                     /gene="ssrA"
                     /locus_tag="VB241_002357"
     tmRNA           4786..5147
                     /gene="ssrA"
                     /locus_tag="VB241_002357"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1878 a          752 c         1082 g         1700 t
ORIGIN      
        1 taacagacta ataatcatga ggtaactaat aacacatatt taacttgtat tattaaactg
       61 gtataataaa tttatgttga aatgaatatt gtatgacagg gtattcactt ttattaaaag
      121 gtaaaattaa ataaaggttt tatagaacgt atttaaatat atgaggagta aacaaatggc
      181 tgatagaacg aataaagaaa ttaaaacagg acgctttatt gcaactgcat caatcgcatt
      241 ctcaatattg ttgattattc attactttgt ttcgttggat aatgcgactg ccaaagcatt
      301 actcaattta acgaatcaaa acacttcaga taaagcgata gattacattt taaacagctt
      361 tagattcact ggtattatgt atattttggc ttatctagca ggcttcatca ctttttggaa
      421 tcgacatact tatgtgtggt ggtttatgtt tgcagtttat gtatcaaata gtttgtttac
      481 gttgattaat ttatcaatca caattcaagc aataaaagct gcacacggtg cgtacttaac
      541 attgccaatt ttaattgtta ttataggttc ggttgcatta gcgatttata tgcttgttgt
      601 ttctatcaaa cgtaaaagta catttaatcg ctagaaaatt gattttaaca atgaaaatat
      661 gatatactac ttgtcgtata taaggaacgg aggacaattt atgcatacat ttttaatcgt
      721 attattaatc attgattgta ttgcattaat aactgttgta ctactccaag aaggtaaaag
      781 cagtggactt tcaggtgcca tcagtggtgg tgctgagcag ttattcggta aacaaaaaca
      841 acgtggcgtc gatttattct taaatagatt aacaattatt ttatcaatat tattttttgt
      901 acttatgatt tgcataagtt atcttggtat gtaaggtccg gcgatgtaaa tgtcgggctt
      961 ttttatttat aattagaata ataaaggact catgttcaat tattagaaac atattgttta
     1021 attaagtacg tttgttaatt gtgaaaaagg agcgttagat ttatgcaaat aaagttacca
     1081 aagcctttct tttttgagga aggtaaacgt gccgtgttat tactacatgg ttttacaggc
     1141 aattcgtctg atgttcgtca attaggtcga tttttacaaa agaaaggtta tacatcgtat
     1201 gcaccacaat atgaaggtca tgcagcacca ccagaagaga tacttcaatc aagtcctttt
     1261 gtttggttta aagatgcctt agatggctat gactaccttg ttgaacaagg ttatgatgaa
     1321 atcgttgttg ctggcttgtc attaggtggc gattttgcat taaaattaag cttaaatcga
     1381 gatgtaaagg gtattgtaac gatgtgtgct cctatgggtg gcaaaactga aggtgccatt
     1441 tatgaaggct ttttagaata tgcacgcaat tttaaaaagt atgagggtaa agatcaagaa
     1501 acgattgaca aagaaatgca tcatttcaaa ccaactgaaa ctttaaaaga attaagtgaa
     1561 gcattagata cgattaaaga tcaagttgat gaggtgcttg acccaatttt agtgattcaa
     1621 gcagaaaacg acaatatgat tgatccacaa tccgcaaatt atatatatga acatgtagac
     1681 tctgacgaaa aagatatcaa atggtatagt cagtcaggac atgttattac aattgataaa
     1741 gaaaaagaac aagttttcga tgatatttat caatttttag agtcattaga ctggtcagaa
     1801 taaaaagaga ttttaacatt agaaaggagg ggcataatga atttaaagca atcaatagaa
     1861 gagattatta atcaacctga atatgaacct atgtcagttt cagattttca agatgcatta
     1921 ggtttaagca gtgccgactc gtttagagat ttaattaagg tgcttgtgga gttagaacaa
     1981 tcaggattaa tcgaacgttc gaaaacagat agataccaaa aaaagcatag ttccaaaggt
     2041 caatcgaaat tgataaaagg aacgttaagt caaaacaaga aaggttttgc tttcctaaga
     2101 cctaaagatg aggatatgga agatatattt attcccccaa caaaaattaa tcgtgcctta
     2161 gacggagata ctgtaattgt agaaatacat caatctaaag gtgaacataa aggtaaaatt
     2221 gaaggggaag ttaagtcgat tgagaagcat tctgtaactc aagttgttgg tacgtatagt
     2281 gaagctagac atttcggctt tgttattcca gatgataaac gtattatgca agatattttc
     2341 attcctaaag gtcaaagttt aggtgcagta gatgggcata aggtactagt tcaaattact
     2401 aagtatgctg atggttcaga taatcctgaa gggcatattt cagcaatatt aggacataaa
     2461 aatgatccag gtgtagatat tttatctatt atttatcaac atggtattga aattgagttt
     2521 cctgatgaag tattagaaga agctgaagca gtacctgatc atattgaaaa tactgaaatt
     2581 aaaggccgtc atgatttacg tgatgaattg acaatcacaa ttgatggtgc ggatgctaaa
     2641 gacttagatg acgcaatcag tgttaaaaag ttagcgaacg gtcatacgca attaactgta
     2701 agtattgctg atgtcagtta ttatgtaacg gaagattcag ctttagataa agaagcttat
     2761 gatagagcga caagtgtata tcttgttgac cgtgtgattc cgatgattcc acatcgatta
     2821 agcaatggta tttgttcatt aaatcctcac gttgatcgtt taacgttaag ctgtcgcatg
     2881 gaaattgatg caagtggtcg tgttgttaaa catgaaattt ttgatagtgt tatacattct
     2941 gattatcgaa tgacgtatga tgcagttaac cagattatta ctgaaaagga tcctaacatt
     3001 cgcgagcaat ataaagaaat tacgcctatg ttagatttag cacaagattt atctaatcgt
     3061 ttgattcaaa tgagaaaacg tcgtggtgaa attgattttg atattagcga agcgaaagta
     3121 ctagttaacg aagatggtat acctacagat gttcaattga gacaacgtgg tgaaggcgaa
     3181 cgtcttattg agtcatttat gttaattgca aatgaaacag ttgctgaaca ttttagtaag
     3241 ttagatgtac catttattta ccgagtgcat gagcaaccta aatcagatcg cttaagacaa
     3301 ttctttgatt ttattactaa ctttggcatt atgattaaag gaacaggcga agatattcat
     3361 ccaacaacac ttcaaaaggt ccaagaagaa gtagaaggtc gacctgaaca aatggtcatt
     3421 tcaacaatga tgttacgttc aatgcaacaa gcgcattatg atgatgtgaa cttgggacat
     3481 tttggcttat cagctgaata ttatacgcat tttacatcac caataagacg ttatcctgat
     3541 ttaacagttc atcgtttaat ccgtaagtat ttaattgaga aatcaatgga taacaaagaa
     3601 gtgaagcgtt gggaagacaa attgcctgag ttagctgaac atacttctaa acgtgaacgt
     3661 cgtgctattg agtcagaacg tgatactgat gaattgaaaa aagcagaata tatgattcaa
     3721 catattggtg atgaatttga aggtattgtc agctcagtag ctaacttcgg tatgttcatt
     3781 gaattgccaa atacgataga aggtatggtt catattgcga atatgactga tgattattac
     3841 cgttttgaag agcgtcaaat ggcattaatt ggtgagcgtc aagctaaagt atttagaatt
     3901 ggtgacacag ttaaggttaa agtgacgcat gttgatgtag atgaacgatt aattgatttt
     3961 caaattgtag gtatgccttt accgaaaaat gatcgatcac agcgcccagc acgaggtaag
     4021 acaattcaag ctaaaacgcg tggtaaatca ttagataaat cgaaatctga tgataaaggt
     4081 cataagaaaa aaggtaagca acgtaaaggt aaaaaccaac gtaaaaatga caaatcagga
     4141 aacagtaaac ataagccatt ttataaagat aaaagtgtga aaaagaaagc acgtcgtaag
     4201 aaaaaataag cagcaatgag gtgagtatga atggctaaga agaaatcacc aggtacatta
     4261 gcggaaaatc gtaaagctag acatgattat aatattgaag acacgattga agcgggaatt
     4321 gtattgcaag gcacagaaat aaaatcaatt cgccgaggta gtgctaacct taaagatagt
     4381 tatgcgcaag ttaaaaacgg tgaaatgtat ttgaataata tgcatatagc accatacgaa
     4441 gaagggaatc gttttaatca cgatcctctt cgttctcgaa aattattatt gcataagcgt
     4501 gaaatcatta aattgggtga tcaaacacgt gagattggtt attcgattgt gccgttaaag
     4561 ctttatttga agcatggaca ttgtaaagta ttacttggtg ttgcacgagg taagaaaaaa
     4621 tatgataaac gtcaagcttt gaaagaaaaa gcagtcaaac gagatgttgc gcgcgatatg
     4681 aaagcccgtt attaagcgat ttagttgctt aatcgggcta tatttgatat agttatatgt
     4741 gcttttgtaa attgcaaaag tatgatttgt ttgatttatt atttcgggga cgtttctgga
     4801 ttcgacaggg gtcccccgag ctcattaagc gtgtcggagg gttgtcttcg tcatcaacac
     4861 acacagttta taataactgg caaatcaaac aataatttcg cagtagctgc ctaatcgcac
     4921 tctgcatcgc ctaacagcat ttcctatgtg ctgttaacgc gattcaacct taataggata
     4981 tgctaaacac tgccgtttga agtctgttta gaagaaactt aatcaagcta gcatcatgtt
     5041 ggttgtttat cacttttcat gatgcgaaac ttttcgataa actacacacg tagaaagatg
     5101 tgtatcagga cctctggacg cgggttcaaa tcccgccgtc tccatatttg tagcctacaa
     5161 cctttgcgga tgtgggcttt tttatttgtt ttttatctct tcttgtgagg aaggttaaat
     5221 tagctgtgaa cgttgatatg atagtatctg attaggttca aattttcatg aatgaaaaat
     5281 taatacttgt gtacatataa gattttgcgt taagggttga aagaatatgt gtcaaataag
     5341 tgtcaaaaaa gttgagcttt gagttttaaa atgtaaattt tatttgcaaa attaattaag
     5401 ctaaagaagt at
//
LOCUS       DATTQF010000152         5379 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.152, whole genome shotgun sequence.
ACCESSION   DATTQF010000152 DATTQF010000000
VERSION     DATTQF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.152"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            203..1054
                     /locus_tag="VB241_002358"
     CDS             203..1054
                     /locus_tag="VB241_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP1245611.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNSDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            complement(1288..1482)
                     /locus_tag="VB241_002359"
     CDS             complement(1288..1482)
                     /locus_tag="VB241_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP1245612.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(2298..3119)
                     /locus_tag="VB241_002360"
     CDS             complement(2298..3119)
                     /locus_tag="VB241_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP1245613.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGSKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGNVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(3137..3832)
                     /locus_tag="VB241_002361"
     CDS             complement(3137..3832)
                     /locus_tag="VB241_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245614.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3825..4850)
                     /locus_tag="VB241_002362"
     CDS             complement(3825..4850)
                     /locus_tag="VB241_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP1245615.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIILITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHAVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSIDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(4939..5042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5100..5378)
                     /locus_tag="VB241_002363"
     CDS             complement(5100..5378)
                     /locus_tag="VB241_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP1245616.1"
                     /translation="MKDVTTFYEEDKHLIYGYTPTCGTCKVSERMLDITNEILQLPLL
                     KIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFQSVTDLLENLK"
BASE COUNT         1625 a          966 c          780 g         2008 t
ORIGIN      
        1 aacttaaacc ctttttatta tttttaataa ctctaaagta caactctaat ccgctctctt
       61 taaaaatata aatgataata agaacacata atttctcaat ggattttatg aatttaaaat
      121 atgttatcat ttcactagga catttgtaat atggtatgat gctatttatg attttattcc
      181 aatcgtttga aaggagttat tcatgcaaca atttaaatgg attaatattc taaaaggttt
      241 tgctatggga acgagtgact tggtacctgg tgttagcggt gggactattg cattattgtt
      301 aggtatatac aatcaattta tcgcttcaat tagtggcata ttctcacgac gtttttggcc
      361 aagttttaca tttttaatcc ctattataat tggaatgtta ctggcaatgg gatcactaag
      421 taatcttttt aattatttat taagccaaca ccatattcca actatgtttt tctttggtgg
      481 attaatcatt ggtatcgttc catatttatt gaaaatttct aactataaaa cgtcatttac
      541 aactaaacat tacatgatgg ttatagctgg tatcgctatc ttaattgtta ttacattaat
      601 gaataatagc gataaacatg ctggagaaac acttactcta tcaactggtc ttattattaa
      661 atactttatt gcaggtatgt gtgcatctag tgctatgtta ctcccaggta tttcagggtc
      721 atttatgtta ttagtatttg gcgtctatgg tacggtcatg ctagcaatat ctgaagttgt
      781 taaacttaac tttgccggtc ttcctatatt acttgcagtc ggatttggcg tgcttgctgg
      841 attcattatt tcaagcaaaa ttatccagta tttcctaaca catcataaac taatgacttt
      901 tgcattaatc attggttttg ttgttggctc cctctttgca gtatttccag gtttaccaac
      961 taatatcgtg atgtggttcg tttcattagt agtatttatc atcggtttta ttgtaagttt
     1021 gactttaggt cgaattaccg ctgaaaatga ataatcattt taggggtgat aaaatatatt
     1081 cagtatctcg ttaatcatat gcaatatatg tctcgttttg tatacatatt attggatatc
     1141 attaacgagt ttttatttag tttttttcta acattaaaat aattattaat aaattttggt
     1201 atccactttt ttaatacaaa aaaccaccta gccgatggtt ataccgacta agtggcatgt
     1261 gcagtgtatt aggaatataa agtaaaatta ctctcctttg ttacctttta ctttatcaat
     1321 aaaatcagtt gctttttctt ttgcattttc aacgaattct ttcgctttac cagaagcttt
     1381 atcttcttta ccttcgtttt ctaaattttt attatcagta acattaccta ctgtttcttt
     1441 aacattacct tttgcttgtt caaatttact ttcgtctgcc ataataaatg cctcctcgga
     1501 ataattaaat gttatatata atacttaccc actgaaaaat tatctaaaca ttttacttaa
     1561 ataatttttg atattgattt gacgtcattt tataactagc gaaatagatt catcattaac
     1621 ttgagggagc ggaactaaaa taataaagaa tcactaatga tttattatga aatggttctt
     1681 gggagtggga ctgaaatgat attttcgcaa aatttatttc gtcatccaac cccaacttgc
     1741 attgtttgta gaatttcttt ttgaaattct ctgtgttggg gccccgcccc cccaacttgc
     1801 acattattgt aagctggctt ttcgtcagct tctgtattgg gaccaacccc aactcgcatt
     1861 gtttgtagaa tttcttttcg aaatcctctg tgttggggcc ccgccaactt gcacattatt
     1921 gtatgctggc tttccatcag cttctatgtt gggcccaacc ccaactcgca ttgcctgtag
     1981 aatttctttt cgaaattctc tatgttgggg ccccgcaatt attgtaaaaa ggttgttaca
     2041 atcgtatttt cattcaatca actactgctt atatatcatt gtaaagcttt ggacattgat
     2101 tgaagtcaca gaagcgtata ggcttcactt aatacataat gacttgataa tcatttatat
     2161 atagttattc atctcttatc atattaaatc ttctacattt catattgatt ctaattaaaa
     2221 aaaaccccca tcaaagtttg cattttttca atgtctactt tgatgggagc atatcagtca
     2281 atgagcttaa ttattaatta tttagcagga ataactgcac cgttgtattt ttcattaatg
     2341 aagtcttgaa tatctttaga ttgtaatact tcaattaatg ctttgatttt cttatcatct
     2401 tgatgtcctt ctttaacagc aattaagttt gcataaggat tatctttcgc actttctaca
     2461 gcaatagaat cttttttagg atttagtttt tgttcgattg caaagtttga attaatgata
     2521 acagcatccg cgtcttcatt ttgataaatt ttaggtaaga attctgctga ttgtttatta
     2581 ttaaacttaa tatctttttt attctctgta atatcactaa acttagcatc ttcaattttt
     2641 acgccttttt tgatcttaat taaacctgca tcaacgaaga attttaagaa gcgtccttgt
     2701 tcagctggat tattagacac ataaactgtt gcaccttttg gtaattcttt taaactttta
     2761 tactttttag agtatacagc cataggttct aagtgaacat taccggcact tacgattttg
     2821 taacctttat cctttttctc tgtgtttaaa tatggtgtat gttggaaata gtttgcgtca
     2881 atttcacctt tgtctagtaa tttattaggc gtagtgtaat cgttaattgt tttaatatct
     2941 agttcataac cttttttctc taataatggt tttgcttttt ctaaaatttc agcatgtggt
     3001 gctggtgaag cacctactgt tattttcttg tcgtcactgc cacttttgct tccattaccg
     3061 caagctgcta atacaactgc aaatgttaat actaaaataa gaccaaataa ttttttcata
     3121 aaatgaaacc cccaatttat cgtttatcaa gtttatttgt aagccaatcc ccaatgaatt
     3181 ggattataaa tacaataatt aaaataaaaa ctgttgatac taaaatgaca tcattttgat
     3241 ttcgagtgaa acctgttaag tatgctaaat ttcctaaacc accggcacca attacacctg
     3301 caactgccgt tgaaccaact aaagcgattg ctgtaactgt aatgccagac actagcgctg
     3361 gcatggcttc aggtaagagg actttacgaa ttactgtcca agtattagca cccattgacc
     3421 aggccgcttc gatgacacct ttatcaattt ctttaaaagc aatttctacg agccttgcat
     3481 aaaacggcgc tgcgccaatg atcaatgctg gtaacgcacc tgtcggacca cttatcgttc
     3541 caagtatcaa acttgtaaat ggaattaata ataaaattaa aataataaat ggtatcgctc
     3601 taaataagtt aacaatgaaa gaaacgatag aataaaataa ccttgcacca atagacttac
     3661 ctttagcaga caagaataat aacacaccta aaataagacc gagtataaat gcaaatatag
     3721 ttgagacgac tgtcatgtat agtgtttcga ctattgcagt ccaaacttct ggccactgaa
     3781 tattaggcat tgtaatcatt tcatttataa tttcactaaa tgatttaccc atgtcttaac
     3841 acctccattt taacttgtcg ctcaattaac tctttttcga attttccgaa atctatactt
     3901 gaaatatatg gaatatgcag aactaaaaag ccgactgttc cattttttgt atttttaata
     3961 tttgcttcta aaatattaat tttaatatca taggcagttg atagactcga tacaataggc
     4021 tcggttgttg ttgaaccagc gaaaactaat ctaacgatat aagcatcttt ttctaatggc
     4081 tctaattctg ttaaagatgt ttcgaaatca tcatttaaat cttctttcac aaatcgtttt
     4141 gtcacagcgt gttgcggatt ttcaaaaacc tgtgtcaccg gtccatgttc tatcacttta
     4201 ccactttcca taactgcaac ttcatcacaa atacgacgaa tgacatgcat ttcatgcgta
     4261 attagtataa ttgttaaatt ttgttgttct ctaattttta gtagtagatc taaaatttca
     4321 tctgttgttt gcggatcaag tgcacttgtt gcctcatcac aaagcaagac tgttggatca
     4381 ttagctaacg ctcgtgcaat cccaacgcgt tgcttttgtc cgcctgataa ctctgatgga
     4441 taagcctttt ctctaccttt taaaccgacg agttcgacaa gttctaatgc tttttgcttt
     4501 gctctccttc tagggacacc tgcaatttca agcggaaaca taatattttt taacacagtc
     4561 cttgaccata acaaattaaa atgttggaag atcatactta ctttttgtct ttttgctctt
     4621 aatccatttt tggacaattg gcctatatgg tctccatcta taataacttc acctgatgta
     4681 ggcgcttcta aatgattaaa cattcgaatc aaagtacttt ttcctgctcc agaaaaacca
     4741 atgacgccat aaatcgatcc tgctcgaatc gataaattaa cgtgatctac agcaaggact
     4801 tctttatttt tagtccgata ttctttaaca acttctttta attcaatcac gttgattcct
     4861 ccctgtgttg cttaataaaa taaaaaatgc tttctcaata tcgatagaaa aattgagaaa
     4921 gcaatagtag tattgtttct ctcatcttca aaagttaaaa ctttatgtga attggcacca
     4981 tttctatata agacggttgc cgggcttcgt agggcacatc cctccaccac tctcgataag
     5041 agtttacgca tcatttaatt tgtattaatc ctaacacctt agtaaaattt cgtcaataac
     5101 tattttaaat tttctaacaa atcagtcacc gattgaaatg cataaattcg ttttacttct
     5161 ttatctttat tcatcaacaa taaaatcggc gtagacatga tttgcatatc tttacaaaac
     5221 tgaggataaa agtttaaatc tattttcaat aatggcaact gcaatatttc attagtaatg
     5281 tctaacattc tttctgaaac cttacacgta ccacaagttg gtgtataacc atagattaaa
     5341 tgtttatctt cctcataaaa tgtagttaca tctttgatg
//
LOCUS       DATTQF010000153         5302 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.153, whole genome shotgun sequence.
ACCESSION   DATTQF010000153 DATTQF010000000
VERSION     DATTQF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.153"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(14..220)
                     /locus_tag="VB241_002364"
     CDS             complement(14..220)
                     /locus_tag="VB241_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245617.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLLYHQ"
     gene            complement(286..894)
                     /locus_tag="VB241_002365"
     CDS             complement(286..894)
                     /locus_tag="VB241_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEP1245618.1"
                     /translation="MQLFETMKIDNGHIPRITYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHNGQYRLKIVLHAESEFETIVSPLPEKSSFTAKFQVLPKAVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRIFLINSLREVADVHLCL"
     gene            complement(894..2045)
                     /locus_tag="VB241_002366"
     CDS             complement(894..2045)
                     /locus_tag="VB241_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEP1245619.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFENAESINSTYEHQT
                     TYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEVYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(2029..2622)
                     /locus_tag="VB241_002367"
     CDS             complement(2029..2622)
                     /locus_tag="VB241_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP1245620.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDIINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIT
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            2973..3641
                     /gene="queC"
                     /locus_tag="VB241_002368"
     CDS             2973..3641
                     /gene="queC"
                     /locus_tag="VB241_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEP1245621.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            3643..4062
                     /gene="queD"
                     /locus_tag="VB241_002369"
     CDS             3643..4062
                     /gene="queD"
                     /locus_tag="VB241_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEP1245622.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            4066..4779
                     /gene="queE"
                     /locus_tag="VB241_002370"
     CDS             4066..4779
                     /gene="queE"
                     /locus_tag="VB241_002370"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEP1245623.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVIAQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            4878..>5302
                     /locus_tag="VB241_002371"
     CDS             4878..>5302
                     /locus_tag="VB241_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245624.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDESA"
BASE COUNT         1774 a          888 c          809 g         1831 t
ORIGIN      
        1 ctactcatta aatttactgg tgatacaata aatccattgc tcgcacaaca aaaggcatga
       61 gttcgtgaat taatgcgtat acaaggataa agcttataac agtagtaatt gttgctatca
      121 aacgaacaac atatattcta ttttcagata gcaatcgatt cactaatcga taatttacgt
      181 atacaagaat taacaacagc acaatataaa caataatcat atttggcccc atttaatttt
      241 taaatttgtt ttaacttcat tttctacatt attatcatac taattttaaa ggcaaaggtg
      301 gacatcggca acctctcgta aactatttat caaaaatata cgtatctcat tgttatgata
      361 tttataaatc aattcgtttt tattaaagtc tttttcaaca agtttgtcac tatctattaa
      421 ataatcacgc atgcatcctt ttaagaaatc atctttataa cttggtgtgt accattttcc
      481 atcctcttca atgacaatgt tgccaatatc aaattcaagg accttgccgt cctctgaagt
      541 tagcaatatt aaatctgttt catgattgtg tgctaaatgc tttcgttctg tcgttttatt
      601 atttataaaa gttggattaa ctgctttggg caacacttga aattttgctg taaaactact
      661 tttctcaggt aaaggtgaca ctatcgtttc gaattcgctt tcagcatgta atacgatttt
      721 aagtctatat tggccattgt gatactttgt tgttacatcg tttaattcat ttcgccatgc
      781 atgttcatca aatttaaagt ttaatcgctc agaagaacat tttatgcgat tagtatgata
      841 agtaattcta gggatatgtc cattatcaat tttcattgtt tcaaataatt gcattataac
      901 atctccaaaa tcttggtttt tgcgtaaaat tcatttactt catcttttgg cttagaatca
      961 attgtaatac ctgctccgac tccataaatc gcttgtccat atttatactc aatagtacga
     1021 attggaatat taaaaatcat tttatcatct tcagttggaa gtaatagtcc aattgctccg
     1081 cagtatatac cacgaggtga actttctaat tgtttaatat atttcattgt attcagtttc
     1141 ggtgcacctg taatcgaacc acaaggaaat aacgatgtta aaatttgagt taagtctgta
     1201 ttagttttta aagttccact taccatcgaa gtcatttgaa atacagtttt atatacctca
     1261 atgaaaaata gtttatacac cttaattgag ccactctgtg atattctccc tatatcgttt
     1321 cttagtaaat caacaatcat gacattttct gcacgatctt tcgaagaagt ttgcaatgtt
     1381 ttataatact gttgatcttc agcttccgtt ttacctctag gcattgtccc tttcatcggt
     1441 ttgcttatga taacgttatc gacattgtta aactgtcctt tttgaaaaaa taattctggt
     1501 gagatagacg ctacttggat ttcatctgtt tgtaataacg cagtataatt accattacta
     1561 aattgagtta atcgttcata taaagtacta ataggataat aaatgttgtc cgttaagcgc
     1621 gccgtatagt tcacttgata cgtttcgcct tcaacaattg cttgttggac acgtttaata
     1681 ttagtcatca taacctcaga agattcaaca aatgaaaaat gatgctttga tacataagta
     1741 gtttgatgtt catatgttga atttatgctt tccgcatttt caaaactata agctactgca
     1801 taaatatcat ctttagctaa tgaatgtgta cacataacat gattaaaata ctttgccgct
     1861 tcgtaactta aatataacga aacatatcta ccttgtcgtt gctgcgcttg tgcaaagtgt
     1921 atcacttctc ccacatcagc caacttagtg gcaacatact tctttataaa tccctttaat
     1981 tgaatatggt attgcttata ttcattttca gttaaatagt agcgataatt atattctatt
     2041 ctcacagtaa tcacctacct tcgataaaaa taattcaact tgtcgataac cgtattcact
     2101 caaaatagat tcaggatgat attgcacacc aaaaaccgga aatgtaatat gctcaaatgc
     2161 cataataatt gcttcatcgt tttttgctgt aatctttaag caatttggaa aagtcgctcc
     2221 gtcagcaatt aatgaatgat aacgcattac attgaaattt tgaggcagtc cttgaaaaat
     2281 accttcattg gtatggcgta actgtgtagt atgtccgtgt acaggatgat agccgtgaat
     2341 gatatttcca ccaaaataag acacgataca ttgaaatcct aaacatacac ctagtatagg
     2401 tacacgctga taaaattgtt ctaacacttc attcaagata ggataatcat ccggattacc
     2461 cggcccaggc gaaataacaa ttgcttttgg cttcatatta atgatgtctt ctatcagcag
     2521 attatcaata ccaacaactt gaactgttag tttcgtttga gtcttaatat agtctattaa
     2581 attatatgta aatgaatcat tattatctat gactagaatc attgtatact ccgttctaaa
     2641 tgtgttttat ttttataata tgtattggat gtagctaaaa ctttaaaggc attgtcatta
     2701 tcctgacctt gattttaact actatatggt atattctatt catcgtacat aaatgaatat
     2761 cagaggttcc tagctgaaac cctctataaa aaactagaca ttgaaatttc aaacattcgt
     2821 tggtaaacct ttaatatata ttataggagc aacccatttc tttcgtctat cttttatgga
     2881 gataggcttt ttttatttta taaattaaaa acgggcttat caatgccatt aacctcttta
     2941 ttaatattat taatgatagg agcaattaca aaatggaaag tgtattaaat aatgaaaaag
     3001 ccatcgttgt ctttagtggt ggtcaagaca gtacaacatg tctcttttat gcaaaaaaac
     3061 atttcaaaga agttgaactc gtaacgttta attatggtca aagacatgat actgaaattg
     3121 aagtcgcaaa acaaattgca caagatcaag gaatgaaaca tcacgtttta gatatgtcat
     3181 tattatcaca acttactcca aacgcattaa cacaacatga tatggaaatt actaataatg
     3241 aagatggtat acctaataca tttgttccag ctagaaattt acttttcttg tcgtttgcag
     3301 gtgctctagc ttatcaaatt ggggctaagc atattattac aggcgtatgt gaaacagact
     3361 tttcaggcta cccagactgt cgcgatagtt ttataaaatc aatgaacgta acattaagcc
     3421 tagctatgga caaagatttt gttattcata ctcctttaat gtggttaaac aaagcagaaa
     3481 cgtggaaatt aagtgatgaa ctcgaagttt tagattatat tcgtacaaaa acattaacat
     3541 gctataacgg tatcattggg gatggctgtg gtgaatgtcc agcttgtcat ttacgtcaac
     3601 gtggactaaa tcaatatctt gaaagtaaag gagcgcttta aaatgttaca acaaatctat
     3661 cctagtacaa cgcatccata tcaattcgaa ttaaataaag attttaattt ttcagctgca
     3721 catcacattc cgtgtgaaga agcaggtatt tgtcaaaatg tccatggtca tacttacttt
     3781 gttaatttaa caattgtcgg tgataaacta gatgacactg gctttttagt gaactttagc
     3841 catttgaaaa agatgataca cggtaaattt gaccatcaac tgttaaataa cttacctgct
     3901 tttaaaaaca aaatcccttc aactgaaatc gtagcggaaa caatttatca aattgttaaa
     3961 gaaaatttgg catcgctcga acaccaacca aaatgtattc aagtatttgt aagagaaaca
     4021 ccaacaagtt atgtcgtatt tagaccaaag gaacaggtgt aactaatggc taaaattcct
     4081 gtactcgaaa tattcggtcc aaccattcag ggtgaaggtc gagtaatcgg tagaaaaacg
     4141 atgtttgtga gaacagctgg atgtgattat cgctgtagct ggtgtgattc tgcatttact
     4201 tgggatggta gtgctaaagg cgatataaaa ctcatgaccg ctgaagaaat ttatgatgaa
     4261 ttaaaacgaa ttggtggcga tttatttaac cacgttacaa tttctggtgg taacccagca
     4321 ttaattaaag gtatccaaga gttagttgac ttatttcaag ataaaggtat tttcagcgca
     4381 ctggagacac aaggcagtaa attccaacct tggatgacac aaattgatga tttaacgatt
     4441 agtccaaaac caccaagttc aactatgacg cctgatttaa aaaagttaga tgaagtgatt
     4501 gcacaatgcg taccttcctc attaaactta aaagtagttg tattcgacga taaagattat
     4561 gattttgcca aaatgataca ccaccgttat ccagatattc ccttctattt acaagttggt
     4621 aacccatatt tatcagacag cgtagataat cataccgaaa agttgttaga acgctacgag
     4681 cagttggtag atttagttat gcaaagtaat gatatgaatc acgtttatgt gttaccacaa
     4741 cttcatacat tactttggag taataaaaaa ggtgtataaa ggataactta ttttactttt
     4801 tacaagtcat atctaatgaa taacaatata ttaattgata taatagttga ttgaagtata
     4861 acgaacagga gtccatcatg attgtatata tattaattaa tattgctatc gtcgtactta
     4921 taactggatt caatttatat aggcatcaaa tgcaacattt atcattaagt gcaatgttgt
     4981 tatctattac tataaacgcc tttataaata cattcataat tgataaatat aactttatta
     5041 ctctgtgtac tataacgatg tttatcattt ggacgatact acagttttac attgataaaa
     5101 aattaaaacc tgtatacatt acagaccaaa aatttattgc tatcatatta acgattgttg
     5161 tcagtttaac gcaacgtgtt accgactttt caagtacaca atcaatatat atgtctattc
     5221 catttttagc ccctgccatt ttcatcattg gtggtattat gttatttatt tcaacattta
     5281 atagcttaga tgaatctgct ga
//
LOCUS       DATTQF010000154         5114 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.155, whole genome shotgun sequence.
ACCESSION   DATTQF010000154 DATTQF010000000
VERSION     DATTQF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.155"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..913
                     /locus_tag="VB241_002372"
     CDS             <1..913
                     /locus_tag="VB241_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HEP1245625.1"
                     /translation="ILGYKDEERKKKRATNQRMFNNKKEDLETIIDEFFGEIGQQRPT
                     SIPTLAPKEEKETNIKNANKLKSDTEAAKNDEAKRSLNTHNHKSVSQEVSEQQKADYE
                     RKAEERKARFLDKQKNKKTPVVSLEYDFEHKQRVDNENDKQLVVSEPSKKPTTPPTYT
                     ETTTQLPMPTVERQTQQQIVYKAPKPLAGLNGESHDFTTTHQSPTTSNHTHNHLIEIE
                     ETSALPGRKTGSLVGLSQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRLN
                     AQQKVNTLSAGHQKRFNKQINKVYNGK"
     gene            1264..2289
                     /gene="emp"
                     /locus_tag="VB241_002373"
     CDS             1264..2289
                     /gene="emp"
                     /locus_tag="VB241_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HEP1245626.1"
                     /translation="MKKKLLVLTMSTLFATQFMNSNHANASTESVDKNFVVPESGINK
                     IIPTYDEFKKAPKVNVSNLADNKNFVASEDKLNKIADPSAASKIVDKNFAVPESKLGI
                     IVPEYKEINNRVNVTTNNPASKQVDKQIVAKDPEVNRFITQNKVNHRFITTQTHYKKV
                     ITSYKSTHVHKHVNHATSSIHHHFTIKPSEAPRYTHPSQSQSLIINHHFAVPGYHGHK
                     VVTPGQASIRIHHFCAVPQINSFKVIPSYGHNSHRMHVPSFQNNTTATHQNAKVNKTY
                     NYKYFYTYKVVKGVKKHFSFSKSHGCKIVKPALNIKNVNYQYAVPSNSPTHVVPEFQG
                     ILPAPRV"
     gene            2620..3177
                     /locus_tag="VB241_002374"
     CDS             2620..3177
                     /locus_tag="VB241_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245627.1"
                     /translation="MKRKVLVLTMGVLCATQLWQTNNAKALVTESGVNDTKQFTEVTS
                     EEKVIKDAISKVNESFIYYPQNDLKGLGGEHNDYEKITYSTSSNNVLELSMSSKYVGG
                     KSGAMVGYSEIYSSHFTDRDKRAIRRDHVKEAQNLINDYKYTQIYEDFAKATAKVSTL
                     SQSHQNYLNKQIDKVNNKIEKTEKR"
     gene            3518..4204
                     /locus_tag="VB241_002375"
     CDS             3518..4204
                     /locus_tag="VB241_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEP1245628.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKEQYAKRFFFFATSCL
                     VLTLVVASSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            4561..4761
                     /locus_tag="VB241_002376"
     CDS             4561..4761
                     /locus_tag="VB241_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP1245629.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT         2062 a          736 c          805 g         1511 t
ORIGIN      
        1 gattcttggt tataaagatg aagagagaaa aaagaagagg gctaccaatc aaagaatgtt
       61 caataataaa aaagaggatt tagaaactat tattgatgaa ttctttggag aaattggaca
      121 acaaaggcca acatctatac caacattagc gcctaaagaa gaaaaagaaa caaatataaa
      181 aaatgcaaat aaattaaaat ctgacactga agcagcaaaa aatgatgaag caaaaagaag
      241 tttaaatacc cacaatcaca aatctgtatc tcaagaagtc tctgaacaac aaaaagctga
      301 ctacgaaaga aaagctgaag aaagaaaagc gagattttta gataagcaaa aaaataagaa
      361 aactcctgta gtttcattag aatatgattt tgaacataaa caacgtgttg acaacgaaaa
      421 cgacaagcaa cttgtggttt ctgagccatc aaagaaacca acaacaccgc ctacatacac
      481 tgaaacaacc acacagctac caatgcctac agttgagcgt caaacacagc aacaaatcgt
      541 ttacaaagca ccaaaaccat tagctggatt aaatggtgaa agtcatgatt tcacaacaac
      601 gcatcaatca ccaactactt caaatcacac gcataatcat cttattgaaa ttgaagaaac
      661 atctgcttta cctggtagaa agacaggttc attggttggt ttgagtcaaa ttgattcttc
      721 gcatttaact gaacgtgaga agcgcgtgat taaacgtgaa cacgtgagag aagctcaaaa
      781 gttagttgat aattataaag atacacatag ttataaagac cgattaaatg cccaacaaaa
      841 agtaaatact ttaagtgcag gtcatcaaaa acgttttaat aaacaaatta ataaagtata
      901 taatggcaaa taattaatgc atggctgcaa aggaaataat gagtttgccg taaaaataac
      961 aacattttaa actagcaata aataatatca aagtcatcat ttcaatgatg caatctagta
     1021 tagtccacat tctaaacagg tgtggactat tacttttttc actttatatt acgaaaaaat
     1081 tattatgctt aactatcaat atcaataatt aattttaagc tgaaaaacaa taaaaatgtt
     1141 aagacaacgt ttacttcaag ttaattatta tactgaaaat tctggtatat aatgctgtta
     1201 gtgaatataa caggaaaatt aaattggtta tgatattgag tctatataaa ggagaaataa
     1261 cagatgaaaa agaaattatt agttttaact atgagcacgc tatttgctac acaatttatg
     1321 aattcaaatc acgctaatgc atcaacagaa agtgttgata aaaactttgt agttccagaa
     1381 tcgggtatta ataaaattat tccaacttac gatgaattta aaaaagcacc aaaagtaaat
     1441 gttagtaatt tagctgacaa caaaaacttt gtagcttctg aagataaatt gaataagatt
     1501 gcagatccat cggcagctag taaaattgta gataaaaact ttgccgtacc agaatcaaaa
     1561 ttaggaatca ttgtaccaga gtataaagaa atcaataatc gagtgaatgt aacaacaaac
     1621 aatccagctt caaaacaagt tgacaagcaa attgttgcta aagacccaga ggtgaataga
     1681 tttattacgc aaaataaagt aaaccatcgt ttcattacta cgcaaaccca ctataagaaa
     1741 gttattactt catacaaatc aacacatgta cataaacatg taaaccatgc aacatcttct
     1801 atccatcatc actttactat taaaccatca gaagcaccta gatatacaca cccatctcaa
     1861 tctcaatcgt taattataaa tcatcatttt gcagttcctg gataccatgg tcataaagtt
     1921 gtaacaccag gacaagctag tattagaatt catcactttt gtgctgtacc tcaaataaat
     1981 agttttaagg tcattccatc atatggtcac aattcacatc gtatgcatgt accaagtttc
     2041 caaaataaca caacagcaac acatcaaaat gcaaaagtaa ataaaactta taactataaa
     2101 tatttttata cttataaagt agtcaaaggt gtaaaaaaac atttctcatt ttcaaaatca
     2161 catggttgta aaattgttaa accagcatta aacatcaaaa atgtaaatta tcaatatgct
     2221 gttccaagta atagccctac acacgttgtt cctgagtttc agggtatctt accagcacca
     2281 cgagtataaa aattgacatt aagtttacga gatatgataa atacctatta ttttaaacat
     2341 agtctgcaat ctatgaggtt gtaggctatg ttttttgcag tttatcaata aacacccatc
     2401 aacaaattat accgtttttc tactttaaaa gttggaagta acataatctt aaataaatat
     2461 attattaatt aagataaata taagactcga gattattgtt aatagtttgt tcatcgcaag
     2521 ttaattattg tttctaaaat attggtatat aattttcaat ggcgaagaaa acagggtaaa
     2581 aaagtcggtt tttaaatcaa agcaaataag gagtaaaaaa tgaaaaggaa agtactagta
     2641 ttaacaatgg gcgtactttg tgcgacacaa ttatggcaaa cgaataatgc aaaagcttta
     2701 gtgacagaga gtggcgttaa tgatactaag caatttactg aagtaacatc ggaagaaaaa
     2761 gttataaaag atgctatttc gaaagtcaat gaaagcttta tttactatcc ccaaaatgat
     2821 ttgaagggat taggtggaga acacaacgat tacgaaaaaa ttacatatag cacttcttct
     2881 aataatgttt tagaattatc aatgagttca aaatacgtag gcggtaaatc aggagctatg
     2941 gttggttata gtgaaattta ctcatcacat ttcacagacc gcgacaaacg tgctatcaga
     3001 cgtgatcatg ttaaagaagc acaaaacttg attaatgatt ataaatatac gcaaatatat
     3061 gaagactttg ctaaagctac tgcaaaggta agtacactta gtcagtctca ccaaaattat
     3121 ttaaataaac aaattgataa agtgaataat aagatagaga aaactgaaaa acgctaatac
     3181 aaagtaattt ataagttata catttcgttt ttaaatgaca atttatcccc gtaaatataa
     3241 taaataatct tttcaaattc cgcatagata tagagacgct aataaacctc tttgtctcga
     3301 tatgatagtc tgcaacgatt catgttgtag gctttttaat tttacaaata cgactaaata
     3361 tataagttct gacacctaaa atatagaaaa tacataaaag taagtatagt tattttatta
     3421 taattattaa atttttatta attaattgta aaaatgtaga attataatta attaacgttt
     3481 aatattaaaa ttaactaaaa agaaagaggt gttagttatg acagaatact tattaagtgc
     3541 tggcatatgt atggcaattg tttcaatatt acttataggg atggctatca gtaatgtttc
     3601 gaaagaacaa tacgcaaaga ggtttttctt tttcgctact agttgcttag tgttaacttt
     3661 agttgtagct tcaagtctaa gtagctcagc aaatgcatca caaacagata atggcgtaaa
     3721 tagaagtggt tctgaagatc caacagtata tagtgcaact tcaactaaaa aattacataa
     3781 agaacctgcg acattaatta aagcgattga tggtgatact gttaaattaa tgtacaaagg
     3841 tcaaccaatg acattcagac tattattggt tgatacacct gaaacaaagc atcctaaaaa
     3901 aggtgtagag aaatatggtc ctgaagcaag tgcatttacg aaaaagatgg tagaaaatgc
     3961 aaagaaaatt gaagtcgagt ttgacaaagg tcaaagaact gataaatatg gacgtggctt
     4021 agcgtatatt tatgctgatg gaaaaatggt aaacgaagct ttagttcgtc aaggcttggc
     4081 taaagttgct tatgtttata aacctaacaa tacacatgaa caacttttaa gaaaaagtga
     4141 agcacaagcg aaaaaagaga aattaaatat ttggagcgaa gacaacgctg attcaggtca
     4201 ataatgctca ttgtaaaagt gtcactgctg ctagtggcac ttttataatt tttagatcac
     4261 gttatgattt attatcaaat cagaattaaa aaagtaaatc gtatcaaaat caagtgtatt
     4321 taatattaga aaataaaaat tttaaattta gtattaaaat ggaatattac tatatatttc
     4381 aatgtgtatt atcacagaaa ataaaataat gctttacttc tatatttaaa agtgtataat
     4441 gaaggttaag taataaagag cgtgaagaaa aatgtgagtt atttatatag aatattctcc
     4501 ttttcattta tgaatttgtt acaaaatatt tagtgcaaaa gcacgacgga ggtattcaat
     4561 atgaataacg gtacagttaa atggtttaat gcagaaaaag gttttggttt catcgaaaga
     4621 gaagatggta gcgacgtatt cgtacacttc tcagcaatcg ctgaagatgg atacaaatca
     4681 ttagaagaag gccaaaaagt tgaattcgac atcgttgaag gcgaccgtgg cgagcaagct
     4741 gcaaacgtag ttaaaatgta attttaactt attcaaacag tccttattat agggctgttt
     4801 ttttatgctt taaatcgata gcagttggtg tggtaaaagc actagctgtt atttttttgt
     4861 ccaataaatt taggtggaga tctaacaata tataatggtt ctaaaataaa tcgaactgat
     4921 ggaaaagttt tttacttttc atctgtccga cttttgattt tgaatataaa aaaagcgcca
     4981 atacagaact ttaataatga cgagaattaa agtctgtata tggcgataac aagaagtaat
     5041 gttaaacact caaaatgttt aacaataata ggataccaca tcgcataata tcttactact
     5101 taattaataa ttta
//
LOCUS       DATTQF010000155         5110 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.156, whole genome shotgun sequence.
ACCESSION   DATTQF010000155 DATTQF010000000
VERSION     DATTQF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.156"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            1..1393
                     /locus_tag="VB241_002377"
                     /pseudo
     CDS             1..1393
                     /locus_tag="VB241_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="class D beta-lactamase"
     gene            1383..1763
                     /gene="blaI"
                     /locus_tag="VB241_002378"
     CDS             1383..1763
                     /gene="blaI"
                     /locus_tag="VB241_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HEP1245630.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            2027..2620
                     /locus_tag="VB241_002379"
     CDS             2027..2620
                     /locus_tag="VB241_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1245631.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNAYGCEKIFSDHISGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEEKEDEKQGEVSN"
     gene            2592..4034
                     /locus_tag="VB241_002380"
     CDS             2592..4034
                     /locus_tag="VB241_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEP1245632.1"
                     /translation="MKNKEKYLTNFSEAKRKEATQKYNIIKPFILGKQSLSSISKSKG
                     IALSTLYRWNKLYKEQGLTGLIHNTRVDKGEHKLKQNIIDEIKRLALKNKRNSIATIH
                     RKIANYCIENNFYKPSYKQVYSIIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPN
                     EIWQADHTLLDIYILDQKGNINRPWLTIIMDDYSRAIAGYFISFDAPNAQNTALTLHQ
                     AIWNKNNTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIE
                     RFFQTVNQTFLEQLPGYINNNDTSSDLIDFQNFEEKLRYFLIEDYNQKEHSAIQSTPI
                     NRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKIHSDGIHFQGFRYSNTNLTAYVGEYVL
                     IRYNPNDMAEIRVFYRDEFLCTAISPDLADYSIDIKEIQHARSQRRKHLKQNIASPST
                     TDLIKEEKSYGYSPQETTKNVKKLKRYRND"
     gene            4027..4842
                     /locus_tag="VB241_002381"
     CDS             4027..4842
                     /locus_tag="VB241_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1245633.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWNTIEKQVNHRGWEDLASKTTDDILSVNKIFYTAPAEKQTRLSNDLYSISASI
                     DLGQKLHIVNKYGHDHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSCYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
BASE COUNT         2018 a          666 c          838 g         1588 t
ORIGIN      
        1 tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta aacgaaaatg
       61 aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaata ttgtgattcg
      121 aaaagcagag actattcaat ctccaataac tttttggtat gggaaatata ttattttgat
      181 tcctagttca tattttaaaa gtgtaattga caaaagacta aaatatataa tattgcatga
      241 atatgctcat gctaaaaata gagatacttt acatttaatt atctttaata tcttctctat
      301 tattatgagt tacaatccat tagtacatat tgtaaaaaga aagataattc atgacaatga
      361 agtagaagcc gatagatttg tccttaataa tattaacaaa aatgaattta aaacttatgc
      421 ggaatctatt atggactccg tattaaatgt tccatttttt aataaaaata tattaagcca
      481 ttcatttaat ggtaaaaagt cattactcaa aagaagatta attaatataa aagaagccaa
      541 tctgaaaaaa cagtccaaat taattctaat ttttatttgt atttttactt ttttgctcat
      601 ggtaattcaa agtcagtttt tgatggggca atccataact gattataatt ataaaaagcc
      661 cttacacaac gattaccaaa ttttagataa aagtaaaatt tttggttcaa atagtggatc
      721 tttcgtcatg tacagcatga agaaagacaa atactatatt tataacgaaa aagaaagtag
      781 aaaaaggtat tctcctaatt caacttataa aatttattta gctatgtttg gacttgaccg
      841 acatattata aatgatgaaa attcacgtat gtcatggaat cataagcatt acccttttga
      901 tgcttggaat aaggaacaag atttaaatac agcaatgcaa aattcagtta attggtactt
      961 cgaacgtatt agcgatcaaa taccaaagaa ctatactgcg actcaactca agcaattaaa
     1021 ttatggtaat aaaaatttgg gaagttataa aagctattgg atggaagata gtttgaaaat
     1081 atctaatctt gaacaagtaa tagtttttaa aaatatgatg gaacaaaata accattttag
     1141 taaaaaagca aagaatcaat tatcttcttc attattgatt aagaaaaatg aaaagtatga
     1201 actgtatggg aaaacaggta caggtatagt aaacgggaag tataataatg ggtggtttgt
     1261 aggttacgta attacaaatc atgataagta ttattttgct acacatttat cagatggaaa
     1321 gccatctggg aaaaatgctg aattaatcag tgaaaaaata ttaaaagaaa tgggtgtttt
     1381 aaatggccaa taagcaagtt gaaatatcta tggctgaatg ggatgttatg aatataatat
     1441 ggggtaaaaa atcagtatca gctaatgaaa ttgtagttga aattcaaaaa tataaagaag
     1501 ttagcgataa aacgattaga acattaatca caagactata taaaaaagag attataaaac
     1561 gatacaaatc agagaatatt tatttttact catcaaatat taaagaagac gatattaaaa
     1621 tgaaaactgc taaaaccttt cttaataaac tgtatggagg ggacatgaaa agtttagtgc
     1681 tgaattttgc gaaaaatgaa gaattaaata acaaagaaat tgaagaattg cgagacattt
     1741 taaatgatat tagtaaaaag taattagaga tatgtttcaa acataataca tatgtaaact
     1801 atgcttgact cttattaaat tagttttgtt aataacattt aaaatatatt attaaagatg
     1861 agagtgtaca gaaaataacc attttctgta cactctcttt ttatgccgaa ataagtgagt
     1921 aatacatata ttttcgtgtt caaaaaccca acaaaaaatc tatgttcaca aaagaccttg
     1981 aaattaagtt atggtacact gtatatgtat ataggaggtt tgtattttga aaataggtta
     2041 tgcaagagtt tcaactggat tacaaaatct gaatttacag gaggaccgat taaacgcata
     2101 tggttgtgaa aagatattta gcgaccatat tagtggttca aaaagtaaaa gacctggatt
     2161 agataaagca attgaatttg cgagatcagg agatacgatt gttgtttgga gactcgacag
     2221 actaggacgt aatatggagg atttaatcac attagttaat gagcttaacg aacgaggtgt
     2281 aagtttccat agtttagaag aaaatataac gatggataaa tcaagttcta caggacaatt
     2341 attatttcat ttattcgcag cgtttgcaga atttgaacga aatttaattt tagaacgttc
     2401 ttcagctggc cgaattgcag cacgtgctag aggacgctat ggaggtagac ccgaaaaatt
     2461 aaatcaaaaa gatttaaact tacttaagac actttatgat aatggcacgc caattaagac
     2521 aattgcagag cagtggcaag tttcacgtac aacgatttat cgttacctca ataaattgga
     2581 ggaaaaggaa gatgaaaaac aaggagaagt atctaactaa tttttctgaa gccaaacgta
     2641 aagaagctac tcaaaagtac aatattataa aaccttttat cttgggcaag caatctttat
     2701 caagcatttc taaaagcaaa ggtattgcgt taagtacact ttataggtgg aataaattat
     2761 ataaggaaca aggtcttact ggtctaattc ataatacaag agttgataaa ggtgagcata
     2821 aattaaaaca aaacataata gacgagatta aacgccttgc actcaagaat aaaagaaata
     2881 gtattgctac gattcatagg aaaatcgcta attattgtat agagaataat ttttataaac
     2941 caagttataa acaagtttat agtattatta aagcaatgcc taagtctgtt attgatttct
     3001 ctcaccaagg tgaaaaatac tatcaaaata agtacgattt aatacaaata agagaatcct
     3061 caagacccaa tgaaatatgg caagccgacc atactttgct agatatttat atattagatc
     3121 aaaaaggtaa tatcaataga ccatggctaa ccattattat ggatgactat agtcgtgcaa
     3181 ttgcaggcta ctttataagt tttgatgctc ctaatgctca aaacacagca ttaacactac
     3241 accaagcaat atggaataag aataatacca attggccagt atgtggtata cccgaaaaat
     3301 tctacacaga tcacgggagt gactttacct cgcaccacat ggaacaagtc gctattgatt
     3361 tgaaaattaa cttgatgttt tcaaaagttg gtgtccctcg tggtcgcggg aaaatagaac
     3421 ggttttttca gacagttaac caaactttct tagaacaact tcctggatat ataaacaata
     3481 atgatacttc tagcgattta atagattttc aaaatttcga agaaaaatta cgatactttt
     3541 taattgaaga ttataatcaa aaagagcaca gtgctatcca gagcactcct atcaatcggt
     3601 ggaacagtaa tcattttttt cctaatatgc caagcagttt agaacaactt gatttattat
     3661 tattagaaat acctaaatct agaaaaattc attcagatgg tattcatttt caaggcttta
     3721 gatacagtaa tacaaaccta actgcttatg taggtgagta tgtattaatt cgttacaatc
     3781 ccaatgatat ggctgaaata cgcgtttttt atagagatga atttctttgt acagcaatat
     3841 cccctgattt agctgattat tcaattgata taaaagagat acaacacgca cgtagtcaga
     3901 gaagaaaaca cttaaaacaa aacattgcta gtccaagcac cactgattta attaaggaag
     3961 aaaaaagtta tggttactca cctcaagaaa cgaccaaaaa cgtcaaaaaa ttaaagaggt
     4021 atcgtaatga ctaaaaatca gaatttcatt gaaacgaaag agtataaacg atttgctgaa
     4081 ttttgcgatg cttgtatcaa atatcaatat attgggatct gctatggtca acctggtgtc
     4141 ggaaaaactt tatcttctag gtattacaca aattggaata ctattgaaaa gcaagttaat
     4201 catagagggt gggaagatct agctagtaaa acgactgatg atatattatc ggtgaataag
     4261 atattttata ctgcaccggc tgaaaaacaa acaagattaa gtaatgatct atatagtatt
     4321 agtgcaagta ttgatttggg tcaaaaatta cacatcgtaa ataagtatgg tcatgatcat
     4381 agtaaacatt atagtgatat gtttaaatat attgatttaa ttatagtaga tgagattgat
     4441 cgccttaaag tgcaacattt agagcaatta agagctattt atgatgaaca taatttagca
     4501 atgatattta ttggtatgcc aggtatagag aaaaaactat cttgttatcc tcaactatat
     4561 tcgcgtatag gttttgcaca tgaatttgat aatctaagta aagacgagac gcatcatata
     4621 ttagaatata agtggcaaga tttaggattt gatctaaaac tcgaagattt tactgattat
     4681 gaggcaataa cgacgattat taaaattaca aaagggaatt ttaggctgat tcatcgttta
     4741 tttgcgcaga tagatagaat tatggatata aatggcttag ataaaatcag tacagaagtt
     4801 gtagaaacag ctagagatag tttagtcata ggtattcgtt aatagaaaag caatgtactt
     4861 taatatagga cattgctttt ctcatttact aggtaaaatc attctcatat atcaagtaaa
     4921 gtaacatatg gtggaaaggt ggaacttttt gttttttaat acaaaaaagc gctattgcct
     4981 ctataattta aagtgaccaa acccaaacta aaggagacaa tgcgcctatg aacaatgtta
     5041 tactaaatac ctttgatttg aaagaataaa gcttgcattt tacttggaaa ttttaattat
     5101 taatttaaaa
//
LOCUS       DATTQF010000156         5064 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.157, whole genome shotgun sequence.
ACCESSION   DATTQF010000156 DATTQF010000000
VERSION     DATTQF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.157"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..504
                     /locus_tag="VB241_002382"
     CDS             <1..504
                     /locus_tag="VB241_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245634.1"
                     /translation="AIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQILLEREG
                     DQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNILPSKEGY
                     MLSINKNVYEELERTIENNGYEADLNVRMTYYHNVSHKQQEVILKGQIDRFNTYNNKE
                     IYDLQFI"
     gene            643..1173
                     /locus_tag="VB241_002383"
     CDS             643..1173
                     /locus_tag="VB241_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEP1245635.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPNYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1437..4526
                     /gene="clfA"
                     /locus_tag="VB241_002384"
     CDS             1437..4526
                     /gene="clfA"
                     /locus_tag="VB241_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HEP1245636.1"
                     /translation="MNMKKQEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSM
                     TQTENTSNESKSNDPSSVNAAPKTDNTNVSDSNTTTNTNSDETNVAQNPAQQETTQSA
                     STNATTEETPVTGEVTTTATNQANTPATTQSSNTNAEESVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDISTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNEAVQGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVTKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDAQNTSIKVYKVDNASDLSESYYV
                     NPDNFEDVTDSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD
                     SDSASDSDSASDSDSTSDSDSTSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSVSDSDSDSDSD
                     SESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDLDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDLDSDSASDSDSDSDSDSASDSDSDSDSDSDSESDSDSDSD
                     SDSDSDSASDSDSDSDSASDSDSDSTSDTGSDNDSDSESDSNSDSDSGSNNNVVPPNS
                     PKSGTNASNKNEAKESKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKENKDKK"
     gene            4747..>5064
                     /locus_tag="VB241_002385"
     CDS             4747..>5064
                     /locus_tag="VB241_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HEP1245637.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEENPYKSESLKLNG
                     KRSTTITSDKYEENLDMLISSLSFADYEKYEEPEYKEAVKKYQQKFMAEDDALKNFLV
                     KRKK"
BASE COUNT         1865 a          791 c         1007 g         1401 t
ORIGIN      
        1 gctataggat ctattggaac atttattatg gctttatttt attttgtatc agtttcagtt
       61 caactttatc aaatgaaaat tagctttctg ccagctttag gttttaacca aattttatta
      121 gaaagggagg gggatcaact taatataatg aattccgcta cagaagagca tcatcataaa
      181 gattatatta aactatataa tttaggtggc ggtgctgcta aaaaaattgc aatagaggtt
      241 ttattgggta atgataaagt cattcagaaa aagtacgtga atattttacc tagtaaagaa
      301 gggtacatgt tatcaattaa taaaaatgtg tacgaagaat tagaaagaac gattgagaac
      361 aatggttatg aagctgattt gaatgtacgt atgacttatt atcataatgt aagtcacaaa
      421 caacaggaag ttatattaaa aggtcaaatc gaccgtttta atacttataa taataaagaa
      481 atttatgatt tgcagtttat ctgaaaattg atttaagagg atagttgttt attgcaaaaa
      541 atatcattca attttaatga aataatggcg tcattactat aaaatattac tttatgttgt
      601 aatgcatttt tctataagat agaactaaaa ggaggggcaa agatgcaaat tagacaaata
      661 catcaacatg actttgctca agttgaccag ttaattagaa cggcatttga aaatagtgaa
      721 catggttatg gtaatgaatc agaactagta gaccaaattc gtctaagtga tacgtatgac
      781 aataacttag aattagtagc tgttcttcaa aatgaagttg tagggcacgg tttactaagt
      841 gaagtttatc ttgataacga ggcacaacgg gaaattggat tagtgttagc acctgtatct
      901 gttgatattc atcatcaaaa taaaggtatt gggaagcgat tgattcaagc attagaacga
      961 gaagcaatat taaaaggata taattttatc agtgtattag gatggccgaa ttattatgcc
     1021 aatctaggat atcaacgcgc aagtatgtac gacatttatc caccatatga tggtatacca
     1081 gatgaagcgt ttttaattaa agaattaaaa gtgaacagtt tagcgggaaa aacaggtacc
     1141 ataaattaca catctgcttt tgaaaaaata tgatttcaag ctaggattac attaggcaga
     1201 gttcatatta ataatacaaa ttgtttacaa tgaaatcgcg cattgtcgtt tgcaattttt
     1261 aaaacagaga aaatgaaaaa cttttcgttg taaatattta ttgtagttaa taaaattcta
     1321 aaacaaatta attgccatta tgcaattttg gtgtataatt gcattaatag agattaaata
     1381 tatattaaaa gggtatacag ttaatataaa atgacttttt aaaaagaggg aataaaatga
     1441 atatgaagaa acaagaaaaa cacgcaattc gtaaaaaatc gattggcgtg gcttcagtgc
     1501 ttgtaggtac gttaatcggt tttggactac tcagcagtaa agaagcagat gcaagtgaaa
     1561 atagtatgac acaaacggaa aatacgagta atgagagcaa aagtaatgat ccaagtagcg
     1621 ttaatgctgc acctaaaaca gacaacacaa acgtgagtga ttctaataca acgacaaaca
     1681 ctaatagtga cgaaacgaat gtagcgcaaa atccagcaca acaggaaacg acacaatcag
     1741 catcaacaaa tgcaactaca gaagaaacac cggtaactgg tgaagttact actacggcaa
     1801 cgaatcaagc taatacaccg gcaacaactc aatcaagcaa tacaaatgcg gaagaatcag
     1861 tgaatcaaac aagtaatgaa acgacttcta atgatactaa tacagtatca tctgtaaatt
     1921 cacctcaaaa ttctacaaat gcggaaaatg tttcaacaac gcaagacatt tcaactgaag
     1981 caacaccttc aaacaatgaa tcagctccac agagtacaga tgcaagtaat aaagatgtag
     2041 ttaatcaagc ggttaataca agtgcgccta gaatgagagc atttagttta gcggctgtag
     2101 ctgcagatgc accggctgct ggcaaagata ttacgaatca gttgacgaat gtgacagttg
     2161 gtattgactc tggagataca gtttatccgc accaagcagg ctatgtcaaa ctgaattatg
     2221 ggttctcagt accaaatgag gctgttcaag gtgacacatt caaaataact gtgcccaaag
     2281 aattaaactt aaatggtgta acttcaactg ctaaagtgcc accaattatg gccggagatc
     2341 aagtattggc aaatggtgta atcgatagtg atggtaatgt tatttataca tttacagact
     2401 atgtaaatac taaagatgat gttaaagcaa ctttgaccat gcccgcttat attgaccctg
     2461 aaaatgttac aaagacaggt aatgtgacat tggctactgg cataggtagt acaacagcaa
     2521 acaaaacagt attagtagat tatgaaaaat atggtaagtt ttataactta tctattaaag
     2581 gtacaattga ccaaatcgat aaaacaaata atacgtatcg tcagacaatt tatgtcaatc
     2641 caagtggaga taacgttatt gcgccggttt taacaggtaa tttaaaacca aatacggata
     2701 gtaatgcatt aatagatgca caaaatacta gtattaaagt atataaagtt gataatgcat
     2761 cagacttgtc tgaaagttat tatgtgaatc cagataactt tgaagatgtc actgatagtg
     2821 tgaatattac attcccaaat ccaaatcaat ataaagtaga gttcaatacg cctgatgatc
     2881 aaataacaac accatatatt gtagttgtta atgggcatat tgatcctaat agtaaaggtg
     2941 atttagcttt acgttcaact ttatatggat ataattcgaa tataatttgg cgatcaatgt
     3001 catgggataa tgaagtagca tttaataacg gatcaggttc tggtgacggt atcgataaac
     3061 ctgttgttcc tgaacaacct gatgagccgg gtgaaattga accaattcca gaggattcag
     3121 attctgaccc aggttcagat agtggttcag attctggcag cgattctaat tcagatagcg
     3181 gttcagattc gggtagtgat tctacatcag atagtggttc agattcagcg agcgattcag
     3241 attcagcaag tgattcagat tcagcgagtg attcagattc agcaagtgat tcagattcag
     3301 cgagtgattc agattcagca agtgattcag attcagcgag tgattcagat tcaacgagtg
     3361 attcagattc aacgagtgat tccgattcag caagcgattc agattcagat agtgactcag
     3421 actcagacag cgattcagat tcagatagtg actcagactc agatagcgat tcagattccg
     3481 acagtgactc ggattcagat agcgattccg actcagacag tgactcagat tcagatagcg
     3541 actcagactc agaaagcgac tcagattcag acagcgattc cgactcagat agtgattcag
     3601 attcagacag cgactcagac agtgtttcag attcagacag tgactcagac tcggatagtg
     3661 aatcagactc agacagcgac tcagattcag acagcgactc agactcggat agtgaatcag
     3721 actcagacag tgaatcagac tcagacagcg actcagactc agatagcgac tcagattcag
     3781 atagcgattc cgactcagac agcgactcag actcagatag cgattccgac tcggatagcg
     3841 actcagactc agatagcgat tccgactcgg atagcgattc agaatcagat agtgactcag
     3901 attcagatag cgattccgac tcagatagcg actcagattc cgacagcgat tcagattcag
     3961 acagtgattt agactcagac agtgactcgg attcagatag cgattccgac tcagacagtg
     4021 actcggattc cgacagcgat tcagattcag acagtgattt agactcagac agtgcctcgg
     4081 attcagatag cgattccgac tcagatagtg cctcagattc agatagcgac tcagactcag
     4141 acagcgattc agaatcagat agtgactcag attcagatag cgattccgac tcagacagtg
     4201 cctcagattc agatagtgac tcggattcag cgagtgattc cgattcagat tcaacgagtg
     4261 acacaggatc agataacgac tcagactcag aaagtgattc gaatagcgat tccgattcag
     4321 gttctaacaa taatgtagtg ccgcctaatt cacctaaaag tggtactaat gcttcaaata
     4381 aaaatgaggc taaagaaagt aaagaaccat taccagatac aggttctgaa gatgaagcta
     4441 atacgtcact aatttgggga ttattagcat cattaggttc attactactt ttcagaagaa
     4501 aaaaagaaaa taaagataag aaataagtaa taatgatatt aaattaatcg tatgattcat
     4561 gaagaagtca cctaaaaagg tggctttttt acttggattt tccaaatata ttgtttgaat
     4621 ataattaata attaattcat caacagttaa ttattttaaa aaggtagatg ttatataatt
     4681 tggcttggcg aaaaaatagg gtgtaaggta ggttgttaat tagggaaaat taaggagaaa
     4741 atacagttga aaaataaatt gctagtttta tcattgggag cattatgtgt atcacaaatt
     4801 tgggaaagca atcgtgcgag tgcagtggtt tctggggagg agaatccata taaatctgag
     4861 tcattgaaat taaatgggaa aagaagtact acaataacta gtgataaata tgaagaaaat
     4921 ttagatatgt taatatcgtc attatcattt gcagattatg aaaaatatga ggaaccagaa
     4981 tacaaagaag cagttaaaaa gtatcaacaa aaatttatgg ctgaagatga tgcattaaaa
     5041 aattttttag tgaagagaaa aaaa
//
LOCUS       DATTQF010000157         5053 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.158, whole genome shotgun sequence.
ACCESSION   DATTQF010000157 DATTQF010000000
VERSION     DATTQF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.158"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(29..910)
                     /locus_tag="VB241_002386"
     CDS             complement(29..910)
                     /locus_tag="VB241_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245638.1"
                     /translation="MQNKSKSPFKIALSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(922..1572)
                     /locus_tag="VB241_002387"
     CDS             complement(922..1572)
                     /locus_tag="VB241_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245639.1"
                     /translation="MIKLIFKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLGNLISDITYGFIDPRIRSN"
     gene            complement(1565..2545)
                     /locus_tag="VB241_002388"
     CDS             complement(1565..2545)
                     /locus_tag="VB241_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEP1245640.1"
                     /translation="MENILEVNQVKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKLNKNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            complement(2548..3534)
                     /locus_tag="VB241_002389"
     CDS             complement(2548..3534)
                     /locus_tag="VB241_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245641.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYQNEYKEI"
     gene            complement(3585..>5053)
                     /locus_tag="VB241_002390"
     CDS             complement(3585..>5053)
                     /locus_tag="VB241_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1245642.1"
                     /translation="DKKIHPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWI
                     YSIEVLANKDYEGAYYPSVENIQGAKDYHEGKADHINGLKKIDDYTMQVTFDKKQENY
                     LTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDY
                     WQGKPALDKINLKVIDQAQIIKAMEKGDIDVTNDATGAMAKDAKSSNAGLKVLSAPSL
                     DYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINS
                     FVPSMHWIAADPKELNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHY
                     SGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTD
                     PDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFDKVGIDEKKRKDIYVKWQKYMNDEL
                     PGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA"
BASE COUNT         1584 a          925 c          645 g         1899 t
ORIGIN      
        1 ggatttatgt cccagactct tttattattt aacgtctacc tctaggatta aaagcatctt
       61 taagcccttc ccctacaaag tttatactta atatagttaa tgtaatcatt atagcaggtg
      121 gcatccatat ccacggttta ccacttataa catccccttc ttgagcatca ctcaacatat
      181 taccccaaga tggaattgct ttactaattc cgaatcctaa aaagctcaat cctgattcca
      241 ctacaatcat accggcaaat aacaatgttg cttgtacgat aactacactt aatatattcg
      301 gcaaaagatg tttcaaaata attttatatg tgggtgtacc aattgatttt gctgccaaaa
      361 agtattcatt ttctttttct tgaagtactt taccacgaac aagtcttgca atacctcccc
      421 aacttaatac gataataact agaacaagaa ttatggcaga tccataagga tttttaattt
      481 tatctccaag tgcagcattt aatacaattg caaatattaa aaatggaaat aacataacaa
      541 attcggttat tctcattaat aatgtatcaa caatacctcc aaaataacca gaaatcactc
      601 caactgtaat tccaatcaaa agcattccta ttgtagatgt aattccaatg gataatgaaa
      661 tacgacctgc atataacaaa cgactaaagt tatccctacc accagagtct gtaccaagaa
      721 tgttttgtgc tgtcatttca ccttttatat ttaataaatc ttgttggttc actggaaaag
      781 gagctattaa tggcgctata attgatacaa tagttatgat taataaaaaa ataaccgata
      841 acattgcaat tttattatga ataaatttag acaatgcaat tttaaaaggc gattttgact
      901 tattttgcat tctaatcccc cctagttact tctaattctt ggatctataa atccataagt
      961 aatatcagaa atcaaattac ccagtaaacc taaagtgaat gcaggtaaaa atgtgtgata
     1021 tattttactc atataatatt caaaagtacc ttctttaagg ttaatatcaa cagaaccttg
     1081 aaacgggaac cattgtaatt caaaagaaaa aataaaaatt gcaaatactc ccgcaataaa
     1141 agatggaatg gctaacatca aataattaaa tatttgcaca gtataatccg tcaaactgta
     1201 agaatatcta cctgacgtta ttcctaaagc aaatgagata atataagtaa taattaatga
     1261 catagcaccg agtaatattg tatttggaat tctttcctca ataacatcaa ttaccggcct
     1321 tttatatttg attgactccc ctaaatcacc gtgtataaca tgattgaccc atctaatgta
     1381 ttgatgtggt attgagtcgt tgagacctaa tttttccctt tgtgcattta ttgcttcttg
     1441 ttttatttta ccgttttgta gatctgaaaa tggatctcct ggttgtatga ttgctaatga
     1501 aaatacaact attgaaatta aaattagtaa cgggatcatt aaccctaatc ttttaaatat
     1561 taatttaatc atttaaatca cctactttat ctacgtagaa acaagcgact tgatgttcgt
     1621 taccaatgtc ctttaattct ggcatacttt ttttgcacat atcttgagca attggacatc
     1681 tagtatggaa aggacaacct tgtggcggat tacttggcga aggtaattct ccctttaata
     1741 taattctatt tttatttagc ttatcaattt gtggtattga tgaaatcaac gcttttgtat
     1801 aaggatgttt gggattttca taaatttctt tatcagatgc gatttcaact atatgaccta
     1861 aatacataac tccaatgaca tcacttatat gttttactac acttaaatca tgtgcgataa
     1921 ataaatagct taagttaaat tgttcttgta aatcttttaa taaattcagt acttgagatt
     1981 gaacagatac atctaatgca cttacaggct catcagcaac aattaaactc ggacgcaaag
     2041 ccaatgctct tgcaattccc actctttgtc tctgtccacc tgaaaattca tgtgcatatt
     2101 tataatatgc atcttcactt agaccaacac attttaataa atatagaact tcttttttta
     2161 tttcttcttt tggcaatttt ttataattta aaataggttc tgaaatgata tctccaacca
     2221 tttgcatcgg attcaatgat gcatacggat cttgaaatat catctgatat tgttgtcgtg
     2281 attttctcag ttttttacct tgtaatcttg ttatatcttc accattaaca attattgagc
     2341 ctgaagttgc atcttcaagt ctgataatca ctttacctaa cgttgactta ccacaacctg
     2401 attcgcctac caatccaaaa gtttgtcctt tttttattga aaacgatacg tcatcaacag
     2461 ctttaacgta ctgagttttt tgtaataatc cagttttaat tttgtagtat ttttttactt
     2521 gatttacttc taaaatattt tccattctta tatctcctta tattcatttt ggtataaatg
     2581 acaccgtaca atgacgtctt cacaaacatg cattttagga gattgattat tacaaatatc
     2641 cagttttttg ttacatctat ttgcaaatct gcacttatta acgtgaaatg cttggattga
     2701 tggcacaatt ccttctattg tttcaagtcg tttctgctct tctttaagtt ttggaattgt
     2761 agataataat ttttgggtat aaggatgttt cggattgtgt agtatttctt ttatgcctcc
     2821 aaattctaca atttgacctg catacataac caaaacttta tcgcaaaatt cagcaaccac
     2881 actcaaatca tgtgtaatca tcataattgc catttgcgtt tccttttgca attcttttaa
     2941 taagtctaat atttgcgctt gaatcgtgac atccaaagca gttgtaggtt catcagcaat
     3001 taataatttg ggagaacatg aaatggccat tgcaatcatt actctttgcc tcataccacc
     3061 agataattga tggggatagc tatttaatat ttcatcttgt cttgcaattc caactttttt
     3121 tagtaaatct tttgctaagt tatttgcttc ttttttagaa atttttttat gtgattttat
     3181 agattccaca agttgatttt ttatagtaaa taccggattt aaagcagtca taggttcttg
     3241 aaaaatcata gcaatatcat ttcctcgaat atctagcaat tgcttctcat tgagcgtatc
     3301 gattcgttta ccatcaaata taacttctcc attacttagt ttagatatct tttctggtaa
     3361 taatttaata attgacttac ttaatatgga cttaccgcaa ccagattccc ctactatacc
     3421 caaaacttct cctttagata attcaaaaga aatattttca acagttgcta accattcatt
     3481 atttattttt aatgatgttt ctaaatcttt aacctctaac aatacattat tcataacatc
     3541 cccccatata agtaaacctc gacaaaatcg tcgaggttac attactaagc ttctttagtt
     3601 aaattatata aactttgatc agttccaatt tcaatgtcta agtttcgtac tttatcatta
     3661 actatagtta tcgatttacc ttggaacatt ggtaatccag gtaactcatc attcatatat
     3721 ttttgccatt taacataaat atctttacgt ttcttttcat caatgcctac tttatcgaag
     3781 tctaatgcat catctaaata ttgatctgat tttggtaaaa ctgttctcat ttcattttga
     3841 ggtctatcag tgtggtataa atctgatggg tctggatctg taccacctgc ccatgatctg
     3901 aagtacactt ccatatcttt tgatgcattt gctaagtctt cattatattt accgaattct
     3961 actaacttca catttgtttt caagccaact ttttcccaga aatcttttat cgcagcagtt
     4021 cttggttcaa aagtaggatt tgaacctgaa taatgtttaa agttaatctc aaatttatta
     4081 cctttaggat cttctctaaa tccgtcacca tctcgatctt tataacctaa cttatctaag
     4141 atttttttag ctttttcagg atcatattta taatcgttta gttccttagg atcagctgct
     4201 atccaatgca ttgatggtac aaaactattt atttcactag cgtaaccatt gaaaaatgct
     4261 ttgatccatt tttctctatc aattgcataa agcattgctt tacgtaattc tttgtcttca
     4321 tattttggtc tcactttacc agttttatta gcttttttat cgtaatcatg agatacgaat
     4381 cctattaaac cgtagtctaa gcttggcgca gataatacct tgagaccagc attagatgac
     4441 ttagcatctt ttgccattgc accggtagca tcattcgtaa catcaatatc gcctttttcc
     4501 attgccttaa taatttgagc ttgatcaata acttttaaat tgattttatc taatgcaggc
     4561 ttaccttgcc aataatcatc aaatttaaca agttgaacag cctcacctgg gacgattttc
     4621 ttaactttat atggtccaat accaatagga tattttcgga ttttatctga tttcgctaaa
     4681 tctttaattg gtacatctga taaatatttt ttacttaata aaggtccagt aataaatcct
     4741 gttaagtaat tttcttgctt tttatcaaat gtaacctgca ttgtgtagtc atctattttc
     4801 ttcaatccgt taatatgatc agcttttcct tcatgataat cttttgcacc ttggatattt
     4861 tctacacttg gataataagc accttcgtaa tccttgttag ctaagacttc aattgaataa
     4921 atccaatcat caattttcaa ttcattacca tcatgccatt taataccttt tttaatttta
     4981 aattctattt tcttagccgg ctcgatatcc ttccatgaag caatatatgc tttaggatgt
     5041 atttttttat caa
//
LOCUS       DATTQF010000158         5032 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.159, whole genome shotgun sequence.
ACCESSION   DATTQF010000158 DATTQF010000000
VERSION     DATTQF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.159"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..314
                     /locus_tag="VB241_002391"
     CDS             <1..314
                     /locus_tag="VB241_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEP1245643.1"
                     /translation="VFLGILFFYLNLNRRLALSVFTMNKEKSQKMILLPNLKHFFFII
                     FAYLYFGGFSFFTLYALITIDVQNIIIFICWGAVTMFFFFINIVPIGIKKAHVILLKK
                     S"
     gene            767..1705
                     /locus_tag="VB241_002392"
     CDS             767..1705
                     /locus_tag="VB241_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP1245644.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
     gene            1915..2451
                     /locus_tag="VB241_002393"
     CDS             1915..2451
                     /locus_tag="VB241_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEP1245645.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            2620..3096
                     /locus_tag="VB241_002394"
     CDS             2620..3096
                     /locus_tag="VB241_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245646.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            3136..4431
                     /locus_tag="VB241_002395"
     CDS             3136..4431
                     /locus_tag="VB241_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP1245647.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGKIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            4459..4980
                     /locus_tag="VB241_002396"
     CDS             4459..4980
                     /locus_tag="VB241_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HEP1245648.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
BASE COUNT         1807 a          667 c          916 g         1642 t
ORIGIN      
        1 ttgtatttct tggtatttta tttttctacc taaatttaaa tcgtaggtta gcattaagtg
       61 tttttactat gaataaagaa aaaagtcaga aaatgatatt gctacctaat ttaaaacatt
      121 ttttctttat aatatttgca tatttatatt tcggtggttt ttcatttttc actttatatg
      181 ccctgataac tatagatgtt caaaatataa ttatatttat ctgttggggt gcagtaacta
      241 tgtttttctt ttttataaac attgttccca ttggcattaa aaaagctcat gtaatacttc
      301 ttaaaaaaag ctgaataata aaggagtttt ttaaatctaa aattattaaa tttaatataa
      361 aaattcgact catagataga atgaataaaa atccgaaata tagaattatc aactacaaag
      421 atgaatgttt tataatggat atcccatgtt aatattaaca gttttaattt ctttgttatg
      481 caaatcaaaa cattatagta tttctaattg aatttgcaat ggctttaatg ttgtcgttct
      541 taaatgattt ttcaatttct gattcaaaag tacatgtaat ttaaaggcta acaagcaatc
      601 gcactatgtg aatatcaact taagcaatac ttattttaac tgagttctac ataacatttt
      661 tctaatacac cgcctaaatt caaaccctaa ttctaacatt tggaagttgt attaaatcat
      721 gatagtgtga tagtggaaaa gttataaaga taaggagctg ggtctaatga agcaatttgt
      781 aaacttaggt aaatctgatg ttgaagtgtt tccaatcgca cttgggacga acgcagtagg
      841 tgggcataat ttatatccga acttagatga agaacaagga aaagatgttg ttcgtcaagc
      901 cattaatcat ggtattaatt tattagatac ggcatatatt tatgggccag aacgatcaga
      961 agaattggtt ggagaagttg ttaaggaata tccgcgagag caaattaaaa ttgctacgaa
     1021 agggtctcat gaatttgatg aaaatcaaga agtacatcag aacaatcaac cggaatattt
     1081 aaaacaacaa gttgagaata gtttgaaacg tctacaaact gattatatcg atttatatta
     1141 tattcatttt ccagatgaca acactccgaa agatcaagca gttgcagcat tacaagagct
     1201 taaggaacaa gggaagatta aagccattgg tgtatcaaat ttcacattag atcaacttaa
     1261 agaagcaaat aaagatggtt acgttgatgt tgtacagtta gaatataatt tattgcatcg
     1321 tgaaaatgag gcagtattac aatattgtgt tgatcaccaa atcacattta ttccatattt
     1381 ccctttagca tccggtattt tagctggaaa atatgatgag aacactaaat ttagtgacca
     1441 tcgtactaca cgtcgtgatt ttaaaccagg tgtatttgaa gaaaatgtgc gtcgcgtaaa
     1501 agctttggaa agcatagctg cagcacatca aacttcaatt gcgaacattg tattagcatt
     1561 ttatttaacg agaccagcta tcgatgtgat tattcctggt gcaaaacgtg cagaacaagt
     1621 cattgaaaat attaaagctg cagatatcgt tttatcagat gatgagattc aatatatcga
     1681 tgaactgttt ccaattgaag actaaatgaa ttgtatttaa tggtgacata tgtatctaat
     1741 tcatcatgaa ttagccccgt atcacaaacg actttatata aggcatgacg aaatttcgtc
     1801 aaatttatat aaaagttgta agcgttttac tattcggtag gtaaagctta tgatagtata
     1861 attaggtaac gaaaaacaca gatatttatt aatcaaaagt gaggttaggc taaaatgatt
     1921 actgttttgt ttggtgggag tagaccaaac ggtaatactg cacaattaac aaaattcgct
     1981 ttgcaagatt tagagtatca atggattgac gtgacacaac atcagtttaa accgatacgt
     2041 gacgtgagac atacagcaga gactattact tcatatgacg atgactattt gccgattcta
     2101 gataaaatat tggctagtga tacgattatt tttgcatcac cagtgtattg gtatagcatt
     2161 tcagcaccat tgaaagcatt tatcgaacat tggtcagaaa cattacaaga taaacgatat
     2221 cctaatttta aggcacaaat ggccgaaaag gattttagag ttattttagt tggtggagat
     2281 tgtccaaaaa taaaagcgaa gccagcaatt acgcaaatga aatatagttt agacttttta
     2341 ggtgccactt taaatggtta tattattgga actgctgaaa agcctggtga catcatgaaa
     2401 gacaactatg ccttagcacg tgcaactgag tggaatagta tattgcaata agacatacct
     2461 catactaaac gaagtgattt tttgcttcgt tattttttat ttttaatttt aacggaaaat
     2521 ataaatatat aaattgaata gacaatcatt gtttattaag tattgagcaa gttgtggtat
     2581 aatgccgtta ttaaaacttg ttaataaggg gagaaagtca tgttcaaggt aagacaagca
     2641 actgaaaaag atgttgttca aattagagat gtcgcaacta aagcttggtt taatacatac
     2701 ttaaatatat acgctgcgac aacagttaat cacttgttag aagcttcata taatgaacat
     2761 catttaaaga aaagacttca agaacaatta ttcttagtcg ttgaagaagg taatgacatc
     2821 gttggctttg ctaactttat ttacggtgaa gaattatatt tatcagctca ttatgttaaa
     2881 ccagaatcgc aacatacagg ttatggtaca gcattgttaa atgaaggatt atcacgtttt
     2941 gaagataaat ttgaaggtgt ttacttagaa gtagataata aaaatgaaga agcagtagct
     3001 tactataaag agcaaggttt tacaatctta cgctcttatg agccagaaat gtatggcgaa
     3061 aagttagact tagcacttat gtacaaagca ttttaagtaa attaaatatt atattaaacg
     3121 aaaaggaggg gaataatgac taacgcatat gtagatttaa aattagtaga agaaaaagtt
     3181 tttaaagacc cgatacatcg atatattcat gttgaagatc aattgatatg ggatttaatt
     3241 aaaactaagg aatttcaaag gttacgtcga attagacaat taggaacact gtacctatct
     3301 tttcacacag cagaacatag tcgctttgga cattctttag gtgtgtatga aatagttaga
     3361 cgattaattg atgagtcatt tattggtcat gatgcatggg acaataaaga tagaccgttg
     3421 gcattatgtg ctgcattatt acatgattta ggacatggtc cattttcaca tagttttgaa
     3481 aaaatattta atacagacca tgaagcatac actcaagcga ttattactgg agatactgag
     3541 gtgaatgctg tattacgtaa agtatcgcct gagtttccaa gagaagttgc ggaagtaatt
     3601 aataaaacgc atcataataa attggtcatt tcgatgattt cttcacaaat cgatgcggat
     3661 agaatggatt atttacaacg tgatgcgtat ttcacaggtg tgtcatatgg tgcttttgat
     3721 atggaacgta ttttaagatt aatgcgacct tctaaagatg aagtactaat taaagaaagt
     3781 ggtatgcatg cagttgaaaa ctttattatg agtcgttatc aaatgtattg gcagatttat
     3841 ttccacccag ttagtcgtgg tggagaagtg ctgcttaata attgtttgaa acgcgcaaaa
     3901 cagctttata atgaaggcta tgaatttaag ttgcatccac atgattttat tccatttttt
     3961 gaagagacag ttacgattga acaatatgtt gaactcgatg aagcggtagt tacgtattat
     4021 ttggaaaaat ggacaaaaga agatgatgcc attttaagtg atttagcaag tcgatttatt
     4081 aatcgagact tatttaaata tattccattt gatggctcaa ttattacaat atcagaactg
     4141 caagaactgt ttgaagcagg tggtattaat ccagattatt attttgtgag tgaagcattt
     4201 tcggatttgc catatgacta tgatcgacct gggtcaaatc gcaaaccgat tcatttatta
     4261 agacaagatg gtaagattag agaaataagc aatcaatctt tagtcattca tagtattaca
     4321 ggcattaatc gccaagacta taaattatat tatcctagag aaatggttgc aaagattaaa
     4381 gataagacaa ttagagaagc tattgaaaat ttgattaatg agcttaatta aacagggcta
     4441 aaattgttat cgttaaatat ggaggttata tcattgtctg agaaaaaagg ctttaatttt
     4501 aatatcataa aaaatgaccc tctagatggt cataaaggta caaatattgg ttcaattagc
     4561 ttagacaata ttgcaccagt ttttatcgat gttgctaaca aagaagcatt tattgatatt
     4621 ggaggcatgc atgctcgcgc caaagttgaa aaaggcgtga aatggattac tgataaagct
     4681 gctgttgaag gcgatgaagc taaagaatat tggttgtgtt gggtaacaac agaacgtaat
     4741 gaacaaggac catattatgc tggtttaaca gcgtgctatt tattagtgaa taaagcaatt
     4801 cgtcgtggtt ataaaagtat gcctgaacat gttaatatga tggataaatc aatgaaacat
     4861 catattatca ttgatcaaat tggtgacgag aataaagcta ttttaaaaga ctttttaatg
     4921 aaccatgatg aaggtatgtg gaagcattct tctgatgcat tacatcaagc atttaattaa
     4981 atattagaaa ctaaaatttc ccaattaatc tataaagata tgattcattt ct
//
LOCUS       DATTQF010000159         5028 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.160, whole genome shotgun sequence.
ACCESSION   DATTQF010000159 DATTQF010000000
VERSION     DATTQF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.160"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(116..2725)
                     /gene="clpB"
                     /locus_tag="VB241_002397"
     CDS             complement(116..2725)
                     /gene="clpB"
                     /locus_tag="VB241_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEP1245649.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDSDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(2928..4742)
                     /locus_tag="VB241_002398"
                     /pseudo
     CDS             complement(2928..4742)
                     /locus_tag="VB241_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1586 a          935 c          642 g         1865 t
ORIGIN      
        1 gaaatattgg cgtttattaa atcatttaaa aagtatattg ccaatgaaca attataataa
       61 atttcaaatc taaaaaacca agaatgcgat taatcatcac attcttggtt caattttatt
      121 catgaatttt ttcaacatta aacgttaagt tattgtctga atttaaatta actttaatag
      181 ttgtaccttc tgggaatccc tctttaatca tcatacgtgc taatggtgtt tcaatttggc
      241 gttgtacaaa acgttttaat ggtcttgcac cgtattgagg ttcataagcc tcttgaccta
      301 gccaagcttt agcatcatca gaaacttcaa ttgagattcg ttgttctagt aatcttatat
      361 ttaattgcgt taatattttg tctacaatca tactcatgtc atcaatagat aaaggtttaa
      421 ataatacgat gtcatccata cgattcaaaa tttctggttt gaaatatgca tttaaacttg
      481 tcataacagc tttttctgtt gattctgtaa tttcaccagt ctcttttacg ttttctagta
      541 aaacttgaga tccaatatta cttgtcataa taataatagt atttttaaaa tcaacgctac
      601 gtcctttaga atcagttaaa cggccttcat ctaaaatttg caataataca ttaaagacgt
      661 cagtatgcgc tttttcaacc tcatctaata aaataactga gtatggatta cgacgaaccg
      721 cttcagttaa ttgaccccct tcatcatgtc caatatatcc tggaggtgcc cctatcaatc
      781 ttgatactgc atgtttttcc atatattcac tcatatcaat acgaatcata tgtttttcag
      841 aatcaaataa tgatgcagct aatgatttag ctaattcagt tttacctacc ccagttggac
      901 ctaggaataa gaaactacca ataggtctgt ttggatcttt aatacctgct ctagctctaa
      961 ctactgcatc tgaaaccagg tcaaccgctt tatcttgacc tacaacacgt ttatgcaaga
     1021 tatcacttaa gtgaagtaat ttttcacgtt ctgtttcaac taattttgaa actggtatgc
     1081 ctgtccattg gctgacaata tcaccaattt cttcgtctgt tacaacttca cgaatcattc
     1141 gatctgaatc ttcaccttgc tcatcttgga aattatcctc taattctcta agctcttttt
     1201 ccaattgagg aattgttcca tattgtagtt cagctgcttt ttctaaattg ttatttgttt
     1261 gtgcatcttc caacgcttgt ctactttcat ctagttgcgc acgtttttct tgtaaatttg
     1321 ctattttttc tttttctgat tctacacgag attgaagtgc tgcttgtttc tctttttcat
     1381 tggcaagctc ttcttgtagt tcttgtaatc tctgtttgct cgcattgtca gattcatttt
     1441 taagtgcgct ttcttcaatt tctaattgca tgacacgtct attaacttga tccaattcag
     1501 ttggatttga tcccatttcc gtacgaattg ttgcacatgc ttggtcaact aaatcaatcg
     1561 ctttatctgg taaaaaacga tcagtgatgt aacgatcaga caattcagcg gcagcaacta
     1621 aggctctatc ttgaatacgc acaccatgat atacttcata tcgttctttt aaaccacgta
     1681 aaattgaaat tgtatcttca acatcaggct cactaactgc tactttttgg aaacgacgct
     1741 ctaatgccga atctttttca atatattctc gatattcatt taaagttgtt gcaccaatac
     1801 aatgtaactc tcctcgtgct aacattggtt ttaacatgtt gcctgcatcc atggcaccat
     1861 ctgttttacc agcacctaca agcatatgga tttcatcaat aaataatata attctaccat
     1921 cagactcttt aacttctttt aggactgctt ttaatctctc ttcaaattca ccacgatatt
     1981 tagcgcccgc tactaatgcg cttaaatcta actcaaaaac agttttatct aataatgatt
     2041 ctggcacatc tttcttaact atacgctgcg ctaatccttc aacaattgca gttttaccaa
     2101 cacctggttc accaatgagc acagggttgt ttttagtttt acgacttaaa atacgaatcg
     2161 tatttcgaat ttcttcatct cttcctataa caggatccat tttaccttgt ctaacttctt
     2221 ctactaagtc gcggccatat ttagctaatg cttcgtaatt aacttctgga ttttgtgatg
     2281 tcacgtgatt tccccctctt acttttttaa taatttcttt gataacttct actttattat
     2341 ttatataatg ttttgttgtt tgatcaatgt ccattgccga acgtaaaata tgctccattg
     2401 aaatatattc atcttcatat tctttcatgt atgattcagc cttagttatc aattggtttg
     2461 cttgttggct aatatattga ccatattgta tattgtcacc ttctacagat gcatacgtgt
     2521 ttagtttgtc ttcataagct ttgtttaatt gatctacctc aatatttgct cgttctaaaa
     2581 tacttttata taagctttca ctttcattta aggcagcgct taaaattgcc tcaatttcta
     2641 tattttgtaa tttatgttgc tgactcagtt caactgcttg ttgtaaagca ctttgaacag
     2701 catatgtcat tttatttata tccattaatt tcacctctaa tttgtagagt tggtcaagtt
     2761 gcacttttga ctttgacttt ctttgacctt aattatatta tagtcccaat ataatcattt
     2821 atcaactctt ttacacttaa atttcctaat atttgtaaat aattttttga attaaaaaaa
     2881 tctagctaat gttacgttac acattaacta gacttattaa acatatgtta tttcttttta
     2941 agtgctttta atatttcatc ttttaaagct aggactcctt tgtactctaa atgtacaccg
     3001 tctggtgcaa aatattcact atgtccttgt gatcgcttat accaatcaat taatgtgaca
     3061 ttggactttc gtttcgccgc gtcagctaat aatcgattta catttgcctc ataaattctt
     3121 ggaacacgtg tattaactaa ataaatcttg gcttttccaa attgattaag taaatcgtcg
     3181 agctgtttga cagtaaagtc gccatttgta cctaattcta atacgacttg atcagatgat
     3241 tttttatatt gtgaataatt cgctttaact aaaggtaagg tttgatacaa ttgacgccct
     3301 acttttccat caattctaga cttaggaact gatgacttaa atgactcacc gatatcaacc
     3361 attactgaat caccgattaa aagaggttta atattattat atacatcgga agattccttt
     3421 ttcttctcac ttactaagcc atcaatgtga atgttatcaa ttggtgctat ccgcattgaa
     3481 taattatctt caatagtggt atcaaagcta ttcgcttttt ctcctataat gtctttacca
     3541 tatttattga atgcacctac taaaatcaac ataaatggaa ttaacaaggt tactacaatt
     3601 accattctta taaattgtgg tatataagaa gatcgccaat ttaaagcttt aataccttct
     3661 tttctaaatg gagtttctat aaagcgatat gatagctctg caaatataat tgttaaactt
     3721 atatctataa agtacacata tacaggtatc tgtccgtcta catagtaact atgtacgaaa
     3781 ctaattactg caaaatgcca taaatataga ctataagacc ttttcccgat aaatactaac
     3841 actggatttg aaaatatctt cgctatccat gtagatggat gaacgacact ggcaataata
     3901 aataacgtta atatggatat taaatagaaa ccaccatcat atatccaatt cgtctcatca
     3961 ttaataatga aaaataataa tataagtact ataaatgata aactacctat gctatcaata
     4021 acatatttta caactttagg tggatcattt ttcaatttaa acggcggcca taaaaaagct
     4081 aaaataacac ccagtaacaa tgtctgtaat ctagtatctg taccaaaata cactcgtgaa
     4141 tgatccccat taatactgta gataaacatc attaacccta aagaaattat agatactcct
     4201 caaaaaataa atcctatttt gtatcgcttt ttaattgtta acaataatgt aaccaaaata
     4261 actgggaaaa atatgtaaaa ctgttcttca attgctaaag accataaatg ctttaatggc
     4321 ataaatgaaa attgctcaaa ataattaaca tcttttgcta tataccacca gtttgataca
     4381 taaaatatcg cagcaataat atcatgttta accctaatga tattatctga ttttaataat
     4441 aaggttgctg tccctacaac cattaataaa actatgactg ctggtaataa acgttttaaa
     4501 cgacgtatcc aaaagctttt caatttaatg atacctgtgt catcatactc tttgagtaat
     4561 aagcttgtaa ttaaataacc agagatcaca aaaaatgtat ccacacctaa aaagccacct
     4621 gtcaaccatt gcttatttaa gtggtaaata ataattccta gaacagcgat tgccctcaaa
     4681 ccatcgagcc ctggcatata tctcattttc ttatattttg taaaaccctt tgttttgttc
     4741 atttttgcat tcttcccttt taaaactgtt cttcttagat gcttaattaa atttagttat
     4801 gctgtttaaa agaatattga aatgcatatg tatattattg aattacgaca tcatcaaaat
     4861 catattgact aaaatactgt taaattaaaa aattaccaat gatgattctt atttcgaaat
     4921 ccaatttgta atgcaactcg gcaacttaaa aactatgaag tattatgtat tgtaatataa
     4981 ctgtaatata aattcaattt attatagaat ttttcaagaa aatattca
//
LOCUS       DATTQF010000160         5003 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.161, whole genome shotgun sequence.
ACCESSION   DATTQF010000160 DATTQF010000000
VERSION     DATTQF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.161"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            120..542
                     /locus_tag="VB241_002399"
     CDS             120..542
                     /locus_tag="VB241_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEP1245650.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITNFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDWDIQRSFNDAVGLNLLDLQ"
     gene            729..1346
                     /locus_tag="VB241_002400"
     CDS             729..1346
                     /locus_tag="VB241_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEP1245651.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLGEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            1343..1627
                     /locus_tag="VB241_002401"
     CDS             1343..1627
                     /locus_tag="VB241_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245652.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(1782..3155)
                     /locus_tag="VB241_002402"
     CDS             complement(1782..3155)
                     /locus_tag="VB241_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEP1245653.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFSKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKSKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DPKALYIKTYLPIFNQIDAKYLHDTQRNESNLFELGIELGRHYPRQMVDHQEKRTQVL
                     AAFKALD"
     gene            complement(3241..4794)
                     /locus_tag="VB241_002403"
     CDS             complement(3241..4794)
                     /locus_tag="VB241_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEP1245654.1"
                     /translation="MSEVKKVVKHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKTGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         1669 a          878 c          721 g         1735 t
ORIGIN      
        1 agtttctata ttgtattaca acattgctac atttgacaat atttttttct ttgtaattat
       61 cactattttc catttaattg tataattaat gacatattaa aataaagggg attgatcata
      121 tgaaattgca aaaaagtaat catactatac tactagtgtt agaaaaagga gaagatatcg
      181 ttgaatgtat aacaaacttt gcagatgacc aagacttaac atttacatct gtcagcggca
      241 ttggtgcatg tgatgacgtc gttttaaaat ttttcaattt aacaacgaaa caatatgaag
      301 aaaaacatat tactgagcca ctagaattga cgagcttatt aggtaatatt tctcgtctag
      361 ataatggaca ttttgcacat ttgcatgcta ctttcggtac tcaatcatat gaaactttta
      421 gcggacatct cgctaaagca attgtttctg caactgctga aatcattctt actgtaactg
      481 attgggacat tcaacgttca ttcaacgatg cagtcggttt aaatttactt gatcttcaat
      541 agcactttac tttttagttg aatcaaattt tattacaagt tatcataagt gcttaatcat
      601 aaatgcattc tcgtttatta atatacttca acgagttaaa ttaaattttt aattaggact
      661 tgaatgcgtt tataactatc acatttgaat gaataacttt tttcattaga aagtagtgtt
      721 tattattgat gcaatctcga tacatcgcta gaatttactt tttaatatta tttatcgtct
      781 ccctttttga gactaaaatt tttcaattta tgactttaaa tttgttttta gcatatatac
      841 cttttgagct tagtctatta ttacgattat tcaaaccgaa taaaaagtat gaatggccac
      901 tctttatcat atatagtttt atatttgtgt tacttttacc aaatacattt tatatggtaa
      961 ctgatttaat ccatttgaac caatttcaat tcaactttta cgcaggacta aatttaggtg
     1021 agtggaaata ctttacctat ttactgcttg gtgtcttctt agcggtttat gtgatgattt
     1081 taatctattt tgaaatacta acattgactt ctcaccgttg gcttaataga ttaattgttg
     1141 tcgtactaat gtttttaaat ggattcggaa tttatattgg tcgtttctta cgcttacatt
     1201 ccgtttattt ctttaatgaa cccttacatg ttctaaatca cattctagaa agtctaacgc
     1261 ttaaaactgc tatgtttgta tgttttatgg ttgtgatgca agcagcaatt ctattatttg
     1321 gaaaaggagt gcgtttgaac aagtgacaat aaatataatc gttttaatag tatctatcat
     1381 tgtctttcaa ttgattattg gacacatatg gcatgatatt ggtttaagtt atttaagaag
     1441 tatcttactc atgatgctac catttggact aggtgtattt atacaacaag tgtcgtatta
     1501 cgaacgacaa tatcctaaat ggcaagtgcc tcaaaacata aaagttagat tgaaatatat
     1561 ctatctcgct acatttttag agtatgtcgt actatacctc actttattta cggacatact
     1621 aagataacac caaattaata agaaaactaa atacaactta tatatttgag caaagccaat
     1681 ttctatcaca tcaataatag aaattggctt atttatattg cttaagaact ttaaattctg
     1741 ctccagattt tactcatgcc tgatctatca attcagccga attagtctag cgctttaaat
     1801 gcagctaaaa cttgtgtacg tttttcttga tgatctacca tttgtcttgg ataatgacga
     1861 cctaattcaa tccccagttc aaaaaggttg gactcattgc gttgtgtatc atgcaaatat
     1921 ttagcatcaa tttgattaaa aatcggaaga tatgttttga tatacaaagc tttaggatca
     1981 aagcgttcac tctgtcttat tggattaaac attctaaaat acggcactgc atccgtacct
     2041 gtagaagcag accattgcca tccatgaata tttgatgctg catcatagtc aataaggtgc
     2101 tttctaaaga atttttctcc ccatgtccaa tctataaata aatctttggt taaaaattgc
     2161 gacacaacca ttctcattcg attgtgcata aaaccagttt gtgtcaattc cattattgct
     2221 gcatcaatga ttggaaatcc tgtttgccct tcgcaccatg cattaaaatc cgcttcattt
     2281 tgcgaccatt ttatctgtcg atatttagat ttgaaagctt ggtatgaggt ttctggatac
     2341 tgtgtcatta acacataata aaattctcta aaaatgagtt cacgtataaa tgcttcatag
     2401 tttgcctcat cctcatcata acgcgccaat aaatcattaa aaatttcaat aatatctaat
     2461 aatccatatg ctaaagcaac acttagctga cttgttaaca cttcaggtaa atagtctctt
     2521 ccgctttcgt aattctgtat atcttgatct aaaaaatgtt gccatttatt ctgttcaatt
     2581 atttgtgagg atccaaatgc tatgtcatca aacactaaat catcaggtga tgcaatgacg
     2641 aagtcttcga atgattttaa atcatagtga tatacgtctc tatgcctcaa gtaaggtcgc
     2701 cattttttag agaaactagt aaaaactttg tatggctccc cttgttgatt cattgtttta
     2761 ctcgctttaa agtaatgatt cccacgtatc aaagtaacgg caatgcgtgc ttcattgaat
     2821 aaagagcgtt gatgtaaaat atcatattct tcaaggtgat aactcataat atcacctgca
     2881 atcactactt cagagatatt gttttcttta caaaatgaaa tgatatcgtc ataagacatg
     2941 acataaggtg tgagcttttc tgcgtgcaac gattggtaaa agcgctgcaa agtaccgcga
     3001 taaaactgtg ctttcaaatc agaagctgaa gacatgtcct cttccggaat gataaaataa
     3061 catgcatcaa tgtcttcttt ttgttgataa atatattcaa ataatggatt gtgttccatt
     3121 cgaaacattc gatttaataa cacagctatt gccataaaca aaacaccctt caattatctt
     3181 tttattttat taaacttttt gtcactttaa aataacctag attcttattt atattttaaa
     3241 ttaaagcaaa cctaaccatt tccaataagt catactaaaa actatcacta gaatataccc
     3301 tacaattgtc aatggtatac ctgtcttcaa gaaatctttc acagtaaaag taccagtgcc
     3361 ataagccaac atattttgag gtgcactaac aggtaataag aaaccaaaac taataacaaa
     3421 ttgttgaatt aaaacaaatc ctatagactg gtctcctaag tgtaacgtag aggttagcga
     3481 aataaaaaca ggtattaacg ctgatgataa acttgttgca ctcgcaaagc ccaaatgaat
     3541 caatatatta aaaagcgtga taagtgcaat tgtcgcgata ataggtaaat gttttaaacc
     3601 taaaacacca aaagtttgat cacttaacca ttgagctgct cctgttttca aaagaacgtt
     3661 acctagtgaa atacctacac caaacacgat aattgttccc catggtattt tattttcaac
     3721 atgtttccat gtcatgacac caattttcgg cattaacata acacctaaag caataatagt
     3781 aatggatgca gagtcaatcg gatgtaatac tttttcagtt gaccaaaaca gtaataataa
     3841 catcgatata acaattaaac gccattcacg tgcgctaacg gggccaagtt tatgcaattc
     3901 ttcttttatt aaatctttgc caccttctat tgtattaatt tctggaggca tcactttaat
     3961 catgatgaaa tataaagcta tagacataac tatggaccaa ggcgctgcat ataagaacca
     4021 ctcgccccat gaaacatcaa atcctaattg atggtttata aaattaatcg ctacgatatt
     4081 ttgtgctgcc gccgttttga taccaatatt ccaaattgac acagcttgta ctgaagtaat
     4141 tattaataaa gacgctaact tgctatcttt agaaacttta aatgctgcaa tcatacccag
     4201 caagattggt acaactgccc ctgctctagc tgttgcagaa ggaacgaaaa atgcaagtac
     4261 aattgaaaca ataattgctc caataactat atttctagtt ttattaccaa caattgatag
     4321 cactaaaaga gctagtcttt tatgcaaatt cgtttcttgc atagcagcag ccaaaaataa
     4381 tgcggctgca acgagagcta cagctgatgt tgcaaatcca ctaaatgcta atgataatgc
     4441 atgatttgtt cctagaagat cacttccagc taaaacagca ctgccacttt tcggattacc
     4501 tagcttctcc cctaaatttt gaactggact aaatcctaaa agtaatatca ttaaaccaat
     4561 aattaaagtt gctgacaccg gatatgatac agcttccgtt acccacataa taactgcaaa
     4621 agctaaaata gctagtactg ccttagccat tattggcaga cttgaaggtg ccgggagtaa
     4681 tagtacaact ataagtatta taaaactcat aacaatccaa aatggcttgt attgcttttt
     4741 tgtcttttct ttcttttgtt gttcatgttt aactaccttt tttacttctg acataacagt
     4801 cacccctttg tttaatctta atttaattat aacaattttt ataaacatcc ttttttgatt
     4861 tatgcaaaat acacaaattt atcgaaatat tttgtaaata atattgctag gtgatgagtt
     4921 atctcggtta attacaaaac gatatctagg tttaaatatt agatcataaa aaaactatct
     4981 actgaatatc ctcatagcat cat
//
LOCUS       DATTQF010000161         4995 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.162, whole genome shotgun sequence.
ACCESSION   DATTQF010000161 DATTQF010000000
VERSION     DATTQF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.162"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            55..381
                     /locus_tag="VB241_002404"
     CDS             55..381
                     /locus_tag="VB241_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEP1245655.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            383..862
                     /gene="rsbW"
                     /locus_tag="VB241_002405"
     CDS             383..862
                     /gene="rsbW"
                     /locus_tag="VB241_002405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HEP1245656.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            837..1607
                     /gene="sigB"
                     /locus_tag="VB241_002406"
     CDS             837..1607
                     /gene="sigB"
                     /locus_tag="VB241_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HEP1245657.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            2042..4192
                     /locus_tag="VB241_002407"
     CDS             2042..4192
                     /locus_tag="VB241_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP1245658.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVENAIRGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTAVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVAKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIHSIDKNKDKVSLTMIDPHE"
     gene            4185..4640
                     /locus_tag="VB241_002408"
     CDS             4185..4640
                     /locus_tag="VB241_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP1245659.1"
                     /translation="MNNEILQRMVENLSEEKFGRTFQHRAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1913 a          619 c          969 g         1494 t
ORIGIN      
        1 gatacagcgc ttaaaatgaa gataaatatt tttaataagt aggagtgtaa tgaaatgaat
       61 cttaatatag aaacaaccac tcaagataaa ttttacgaag ttaaagtcgg tggagaatta
      121 gatgtttata ctgtgcctga attagaagag gttttaacac ctatgagaca agatggaact
      181 cgtgatattt atgttaattt agaaaatgtg agttatatgg attcgacagg tttaggttta
      241 ttcgtaggta cattaaaagc attaaaccaa aatgataaag agctatacat tttaggtgtg
      301 tcagatcgta tcggtagact atttgaaatt actggtctta aggatttaat gcatgttaat
      361 gaaggaacgg aggtcgaata acatgcaatc taaagaagat tttatcgaaa tgcgcgtgcc
      421 agcatcggca gagtatgtaa gtttaattcg tttaacactt tctggcgttt tttcgagagc
      481 tggtgctaca tatgatgata ttgaagatgc caagattgca gttagtgaag ctgtgacaaa
      541 tgcagttaaa catgcataca aagaaaaaaa caatgtgggc attattaaca tatattttga
      601 aattttagaa gataaaatta aaattgttat ttctgataaa ggtgacagtt ttgattatga
      661 aacaactaaa tcaaaaatag gtccttacga taaagatgaa aatatagact ttttacgtga
      721 aggtggccta ggtttatttt taatcgaatc tttaatggat gaagtgacag tatataaaga
      781 atctggtgtg acaatcagta tgactaagta tataaaaaaa gagcaggtgc gaaataatgg
      841 cgaaagagtc gaaatcagct aatgaagttt cacctgagca aattaaccaa tggattaaag
      901 aacaccaaga aaataagaat acagatgcac aagataagtt agttaaacat taccaaaaac
      961 taattgagtc attggcatat aaatattcta aaggacaatc acatcacgaa gatttagttc
     1021 aagttggtat ggttggttta ataggtgcca taaatagatt cgatatgtcc tttgaacgga
     1081 agtttgaagc ctttttagta cctactgtaa tcggtgaaat caaaagatat ctacgagata
     1141 aaacttggag tgtacatgtt ccgagacgta ttaaagaaat tggaccaaga atcaaaaaag
     1201 tgagcgatga actaaccgct gaattagagc gttcaccttc tatcagtgaa atagctgatc
     1261 gattagaagt ttcagaagaa gaagtgttag aagcaatgga aatgggacaa agttataatg
     1321 cgttaagtgt tgaccattcc attgaagctg ataaagatgg ttcaactgtt acgctattag
     1381 atattatggg acaacaagat gaccattatg acttaactga aaaacgtatg attttagaaa
     1441 aaatattacc tatattatct gatcgcgaac gagaaatcat acaatgtacg tttattgagg
     1501 gtttgagtca aaaagagaca ggtgagcgta tcggtttaag tcaaatgcat gtttccagac
     1561 ttcaaagaac ggcaattaag aaattacaag aagcagcaca taaatagaat ttgcttatta
     1621 ataatgcgtt ttataatgaa aaatccatat aattatccct tgattattaa attgaaatcg
     1681 agggatattt ttaatttaat taagattttc gaattaatac attattaacg tagtttaatg
     1741 tgtatccaca taaatgtcgc gatatagtat taataattta agtgaagaag atatctaatt
     1801 gtcagtttaa ttaagtggat tgctattaga ataaaaaaag taggtttaga ttatgaaatt
     1861 taggaaatga tggtgtgtca ttttcaataa tcttagtgcg ttttaagata tagtatgacc
     1921 taataattcg tttttaaagt tttggaagtt aaaattgcat taagtatcat accttaatag
     1981 aagtatttta gaatatgtta aaataaatga gtaaatttaa gaaaaagtgt gggttaagta
     2041 aatggacaat caattgatta attcaatcat agagaaatat caatttagta aaaaacaaat
     2101 tgaagcagtg ttaacactgc ttgaagaaaa aaatacagta ccatttattg cgaggtatcg
     2161 aaaagagcaa actggtggac tagatgaagt tcaaataaag caaattgatg acgaatacca
     2221 atatatggtc aatttacaaa aacgtaaaga agaagttatc aaaaatatag aacagcaagg
     2281 attacttact gaggaattaa agaaagatat tttaaaacag aacaaattac aacgtgttga
     2341 agacctatat agacctttta aacaaaagaa aaagactagg gcgactgagg cgaaacgtaa
     2401 aggattagaa ccattagcga tatggatgaa ggcacgtaaa catgaagtat caattgaaga
     2461 aaaagcacaa caatttataa atgaagaagt gcaatcggtt gaaaatgcta tcagaggtgc
     2521 gcaagatatt attgctgaac aaatttcaga taatcctaaa tatagaacaa aaattttaaa
     2581 agatatgtat catcaaggtg tgttaactac atctaaaaag aaaaatgctg aagatgaaaa
     2641 aggtattttt gaaatgtact atgcatatag tgagccaatt aaacgcattg ctaatcatag
     2701 agttttagct gtgaatcgtg gcgaaaaaga gaaagtatta tctgtaaagt ttgaattcga
     2761 tacgacagca gtagaggact tcattgcacg tcaagaaatc aatcataata atgtaaatcg
     2821 cagttatatt ttagaggcga ttaaagatag cttgaaacgc ttaattgtcc cttcgataga
     2881 gcgtgaaatc catgctgatt taactgaaaa agctgaaaat catgcaatag atgtttttag
     2941 tgaaaactta agaaatctat tactgcaacc tccaatgaaa ggtaaacaaa tattaggcgt
     3001 agatcctgca tttagaacag gttgtaaatt agcagtcatt aacccatttg gtacttttat
     3061 agcaaaaggt gtgatttatc cgcatccacc agttgctaaa aaagaagcag cagagaagga
     3121 ttttgtacaa atggttaaag cgtatgatgt gcaattaatt gcaattggca atggtacagc
     3181 aagtcgtgaa acagaacaat ttgttgcaga tttaattaaa aagcatcagt tgccagtaca
     3241 attcatcatt gtcaatgaag cgggcgcctc agtatactca gcatcagaaa ttgctagaga
     3301 tgaatttcct gattttcaag tggaagaaag aagtgccgta tcaattggac gacgcgttca
     3361 agatccatta agtgaattag ttaaaatcga tcctaaatct attggtgttg gacaatacca
     3421 gcatgatgtt aatcaaaaag cactagaaaa tgcattaact ttcgttgttg aaacagcagt
     3481 taaccaagta ggtgttgatg tcaatacagc atcatcatca ttattacaat atgtgtctgg
     3541 tctaagttcg caaattgcta aaaatataat tgcatataga gaagaaaatg gcgcaataaa
     3601 gcataataaa gagttaagca agattaagcg tttgggtgct aaaacttttg agcagagtat
     3661 aggattttta agaatagtag acgggtcaga gccattggat aatacatcga ttcatcccga
     3721 aagctataaa gtaacgtatc agttattaga taaactcgga tttggtggta atgatttagg
     3781 tagtgatgca ttaaaagcta aattaaattc attggatatg gatgaactgg cgattgaatt
     3841 acaagtcggt gtacctacat tagaggatat tattaaatcc ttgaaagcac ctaatcgaga
     3901 tccaagggac gagtttgaaa caccaatcct taaatccgat gtgctatcaa ttgaagattt
     3961 aaaagaagga atgaaattga gtggcacagt tagaaatgta gtagattttg gagcatttgt
     4021 agatataggt gttaaacaag atggacttgt ccatgtttcg aaactttcta agaaatttgt
     4081 taaaaaccca atggatattg tcagtgttgg tgatatcgta gacgtttgga ttcatagtat
     4141 tgataaaaat aaagacaaag tatcattaac aatgattgat ccacatgaat aacgaaatac
     4201 ttcagcgaat ggtcgagaac ctttcggaag aaaaatttgg acgtaccttt caacatcgtg
     4261 catattttaa taaaaggtta cgtacaacag gcggacgtta tcttttaaag tctcatgata
     4321 ttgaaattaa tccaaaacaa tatgagcatt atggagaaga tgctgttgta aaaattatac
     4381 tgcatgaact gtgtcattat cacttgcata ttgctggtaa aggttatcaa cataaagatc
     4441 aagattttaa acgattgagc caacaggttg gagcgcctag attttgtaac agcattgaaa
     4501 gctatcaaca acgggcaaat tatgaatatt attgcactaa atgtcatgca aaatatatac
     4561 gtatccgtaa agttgatacg aatcgtatgc gttgcggaca ttgcaatgga aaattaagaa
     4621 tgaaaagaca attgaaataa gtacaataca aatagagcta attcatgtat atgtaagatt
     4681 atttatgagt tgtatggaat ttatatagaa tttatcagtg agttattttg attggtaaac
     4741 atagtaattg aataagaaga aattgtgttt ataaaatgtt taaagccgag attgtcgtaa
     4801 tttaatactt cgcaatcgcg gcttttctac ttttaatata ggtaatggca taaattgtga
     4861 cttgatagga tgaaattaaa tagattagat taaaaatctt ggcgtatttt agtaatgaat
     4921 tatatataaa ctttacccaa ttaaagcttt aaagtggcac aaaagataat tatacatttt
     4981 taatgacatt ttgat
//
LOCUS       DATTQF010000162         4965 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.163, whole genome shotgun sequence.
ACCESSION   DATTQF010000162 DATTQF010000000
VERSION     DATTQF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.163"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            180..1052
                     /gene="dprA"
                     /locus_tag="VB241_002409"
     CDS             180..1052
                     /gene="dprA"
                     /locus_tag="VB241_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEP1245660.1"
                     /translation="MIRLFLLKLYWAHFSTKQIHQFLMAYPNVIKEGGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDTEYPVLLKEIYQFPLLLF
                     YRGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEIGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1226..3301
                     /gene="topA"
                     /locus_tag="VB241_002410"
     CDS             1226..3301
                     /gene="topA"
                     /locus_tag="VB241_002410"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP1245661.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3457..4764
                     /gene="trmFO"
                     /locus_tag="VB241_002411"
     CDS             3457..4764
                     /gene="trmFO"
                     /locus_tag="VB241_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP1245662.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLSERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEAAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1893 a          719 c          915 g         1438 t
ORIGIN      
        1 aggtttgatt ataaagtatg ttggaaattg aaatttaaaa ttctttccaa catacttttc
       61 actttttaag tcggtatttt actccttaaa ataacattac atcgacaatg tttttgtatt
      121 agaaatgtaa aacaaaaata gaatatgatt ttgtatgatt aaaaaaagga gttgatttat
      181 tgattagact atttttgctt aagttatact gggcacactt ttcgactaaa caaattcatc
      241 aatttttaat ggcataccct aatgtaatta aagagggggg cagaaaaaaa gatagttatt
      301 tatgtgaatg ggtgaatagg gaagaaaatg ttcatttatt acgtaaatac tatgctttta
      361 taaaacttga tcataacgat attattaaag aactgcagaa attaaaagta agttacatta
      421 catatatgga tactgaatac ccagtgctat taaaagaaat atatcaattt ccattacttc
      481 ttttctatag agggaacatc aaattaataa ataatatgca tcatttggca gtagtaggtg
      541 caagagattc tacaagttat acccaacagt ctttagaatt tttattatca aatgataaaa
      601 gcaaatattt aacaattgtt tccggccttg ctcaaggagc tgatgcaatg gcacatcaaa
      661 tagctttaaa atacaatctc cctacaatag cagttttagc ctttggccat caaacacatt
      721 atcccaaaag cacattagca ttaagaaata aaatagaaga aataggttta gttatatccg
      781 aatatccacc acatacacca attgctaaat atagatttcc tgagcgcaat agaattatca
      841 gcggtttgtc aaaaggggtt ttaattactg aggctaagga acaaagtggt agtcacatca
      901 cgatagattt tgcattagag caaaatagaa atgtttatgt tttacctgga tctatgttta
      961 atcctatgac aaaaggtaat ttattacgta tccaagaagg tgctaaggta gtattaaacg
     1021 ctaatgatat atttgaagac tactatattt aaaactaaaa ataacgagtt gcttcatatt
     1081 tcttttgtta ccctttgtaa tttatttttt aataaaatct ataaaaaata gacagggaaa
     1141 atgatttgtt tagatataaa acgttgacaa aagcaaaatt aagcgtttat catttatctt
     1201 tagtaattag attagcgagg gggaaatgac attggcagat aatttagtca ttgttgaatc
     1261 gcctgcaaaa gcaaaaacca ttgaaaagta tttaggtaag aaatataaag ttatagcttc
     1321 aatgggacac gtcagagact taccaagaag tcaaatgggt gtcgacactg aagataatta
     1381 cgaaccaaaa tatataacaa tacgcggaaa aggtcctgtt gtaaaagaat tgaaaaaaca
     1441 tgcaaaaaaa gcgaaaaacg tctttctcgc aagtgacccc gaccgtgaag gtgaagcaat
     1501 tgcttggcat ttatcaaaaa ttttagagct tgaagattct aaagaaaatc gcgttgtttt
     1561 caacgaaata actaaagacg ctgttaaaga aagttttaaa aatcctagag aaattgaaat
     1621 gaacttagtc gatgcacaac aagcgcgtcg aatattagat agattagttg gctataacat
     1681 ctcgccagtt ctatggaaaa aagtaaaaaa agggttgtca gcgggtcgag ttcaatctgt
     1741 tgcacttcgt ttagtcattg accgtgaaaa tgaaatacga aactttaaac cagaagaata
     1801 ttggactatt gaaggagaat ttagatacaa aaaatcaaaa ttcaatgcta aattccttca
     1861 ttataaaaat aaacctttta aattaaaaac gaaaaaagat gttgagaaaa ttacagctgc
     1921 attagatgga gatcaattcg aaattacaaa cgtgactaaa aaagaaaaaa cgcgtaatcc
     1981 agcaaaccca tttacaactt ctacattaca acaagaggcg gcacgtaaat taaactttaa
     2041 agctagaaaa acaatgatgg tcgcacaaca attatatgaa ggtatagatt tgaaaaaaca
     2101 aggtacgatt ggtttaataa catatatgag aaccgattct acacgtattt cagatactgc
     2161 caaagctgaa gcaaaacagt atataactga taaatacggt gaatcttaca cttctaaacg
     2221 taaagcatca gggaaacaag gtgaccaaga tgcccatgag gctattagac cttcaagtac
     2281 tatgcgtacg ccagatgata tgaagtcatt tttaacgaaa gaccaatatc gattatacaa
     2341 attaatttgg gaacgatttg ttgctagtca aatggctcca gcaatacttg atacagtctc
     2401 attagacata acacaaggtg acattaaatt tagagcgaat ggtcaaacaa tcaagtttaa
     2461 aggatttatg acactttatg tagaaactaa agatgatagt gatagcgaaa aggaaaataa
     2521 actgcctaaa ttagagcaag gtgataaagt cacagcaact caaattgaac cagctcaaca
     2581 ctatacacaa ccacctccaa gatatactga ggcgagatta gtaaaaacac tagaagaatt
     2641 gaaaattggg cgaccatcaa cttatgcccc gacaatagat acgattcaaa agcgtaacta
     2701 tgtcaaatta gaaagtaagc gttttgttcc tactgagttg ggagaaatag ttcatgaaca
     2761 agtgaaagaa tacttcccag agattattga tgtggaattc acagtgaata tggaaacgtt
     2821 acttgataag attgcagaag gcgacattac atggagaaaa gtaatcgacg gtttctttag
     2881 tagctttaaa caagatgttg aacgtgctga agaagagatg gaaaagattg aaatcaaaga
     2941 tgagccagcc ggtgaagact gtgaagtttg tggttctcct atggttataa aaatggggcg
     3001 ctatggtaag tttatggctt gctcaaactt cccggattgt cgtaatacaa aagcgatagt
     3061 taagtctatt ggtgttaaat gtccaaaatg taatgatggt gacgtcgtag aaagaaaatc
     3121 taaaaagaat cgtgtctttt atggatgttc gaaatatcct gaatgcgact ttatctcttg
     3181 ggataagccg attggaagag attgtccaaa atgtaaccaa tatcttgttg aaaataaaaa
     3241 aggcaagaca acacaagtaa tatgttcaaa ttgcgattat aaagaggcag cgcagaaata
     3301 atatttttat ttcctagaga cattttaaga ttgttaaata gaatcattag tgaatcttat
     3361 tttaaagata gtaatggatt aatctaaata agtgcggata atattaacat aacaacataa
     3421 ttaaaagaca taaatgacaa taaaaggagt atagaaatga ctcaaactgt aaatgtaata
     3481 ggtgctggtc ttgccggttc agaagcggca tatcaattat ctgaaagagg aattaaagtt
     3541 aatctaatag agatgagacc tgttaaacaa acaccagcgc accatactga taaatttgcg
     3601 gaacttgtat gttccaattc attacgcgga aatgctttaa ctaatggtgt gggtgtttta
     3661 aaagaagaaa tgagaagatt gaattctata attattgaag cggctgataa ggcacgagtt
     3721 ccagctggtg gtgcattagc agttgataga cacgattttt caggttatat tactgaaaca
     3781 cttaaaaatc atgaaaatat cacagttatt aatgaagaaa ttaatgccat tccagatgga
     3841 tacacaatta tcgcaacagg accacttact acagaaaccc ttgcacaaga aatagtggac
     3901 attactggta aagatcaact ttatttctat gatgcggctg ctccaattat tgaaaaagaa
     3961 tctattgata tggataaagt ttacttaaag tcccgttatg ataaaggtga agctgcatat
     4021 ttaaactgtc ctatgactga ggatgaattt aatcgctttt atgatgcagt attagaagct
     4081 gaagctgcgc ctgtaaattc atttgaaaaa gaaaaatatt tcgagggttg tatgcctttt
     4141 gaagtaatgg cagaacgcgg acgcaagaca ttactatttg gaccaatgaa accagtagga
     4201 ttagaagatc caaagactgg gaaacgtcct tatgcggtgg ttcaattaag acaagatgac
     4261 gctgctggca cactctacaa tattgtcggc ttccaaacac atttaaaatg gggagctcaa
     4321 aaagaagtca ttaaattaat tccaggttta gagaatgttg atattgtcag atatggtgtg
     4381 atgcatagaa ataccttcat taactcaccg gacgtattaa acgagaaata tgaattgatt
     4441 tcacaaccaa acatacagtt tgcgggccaa atgactggtg ttgaaggtta tgtagaaagc
     4501 gcagctagcg gcttagttgc aggcatcaat cttgcgcata aaatattagg caaaggtgag
     4561 gtagtatttc cgagagaaac tatgattgga agtatggctt actatatttc tcatgctaaa
     4621 aacaataaga atttccaacc tatgaatgct aacttcgggt tattaccatc tttagaaact
     4681 agaattaaag ataaaaaaga acgctatgaa gcacaagcta atagagcttt ggattactta
     4741 gaaaatttca aaaaaacttt ataaaatagt tagaaagact agatatgcta ttcattctta
     4801 agtcatcaac gagtaagtaa tgactttcta aatggaaaat acttatccta gtctttttaa
     4861 ttttggaatt gttacgtatt tctgacaatt tagaattcgt attcaaataa atatctaaat
     4921 aaataacacg caataagttg attgatgtaa catgtaagtg aatgt
//
LOCUS       DATTQF010000163         4913 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.164, whole genome shotgun sequence.
ACCESSION   DATTQF010000163 DATTQF010000000
VERSION     DATTQF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.164"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(177..1148)
                     /locus_tag="VB241_002412"
     CDS             complement(177..1148)
                     /locus_tag="VB241_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEP1245663.1"
                     /translation="MTETKAIDTHAHLWNEDYLDKLGKLGSQGTEVAKGINQSASKED
                     LEKRFKMMDDAGVDLQIISATPQSPQWGTKEEAHQSAQEINDLYERLVQQYPDRFLAY
                     GAVSLPYVDQAIEEAQALLKKDEFVGIAIPTIVKDKVSVADKQFEPFFAAMNELNATI
                     YIHPTGCGAQSPLVNDYQLEWVIGAPLESTFITLQLIKNEIPQKYPNIKFHISHLGGA
                     LPFFMTRIKDNYEDWNAFNIDPYEVLNRQFWYDTANFHEPSLINSIETFGKDKFMMGS
                     DFPYFQDEKYTRGVDYIKNSNIDPDTINGILRGNAIEFYQIEENHNH"
     gene            complement(1201..1572)
                     /locus_tag="VB241_002413"
     CDS             complement(1201..1572)
                     /locus_tag="VB241_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEP1245664.1"
                     /translation="MLLRHVINTYVGSKIFKSSLPKVQNSDEMAQQFEQGFGLSRRSM
                     RLAGAFEMIGSLLLFTSIFGKLGKKLVVVGTIMINIVMGTAIYNHYKAGHGYKGAQAA
                     SKFFFLNVLSLLEVLSLTRNK"
     gene            complement(1592..2953)
                     /locus_tag="VB241_002414"
     CDS             complement(1592..2953)
                     /locus_tag="VB241_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEP1245665.1"
                     /translation="MKQRNRVIAVVMIGAFVGVLNQTLMTTILPEIMKDFTVSSSTAQ
                     WLTTIFMLVNGIMIPITAFLIERFTLRSLFFNATCFLMIGSFICMLGINFPMLLLGRS
                     IQALGAGILMPLTQTLLFIMFPPEKRGMAMGMFGLVIGFAPAIGPTAAGWFVNIFDWR
                     YLFLVVLLIGMIDFVFGYLSLPNITELSKPNLDKLSVILSTVSFGGLLYGFSTAGNLG
                     WSHPMVYITIIAAIVILTVFIFRQLKLESPLLEFRVFKYNDFSVAMILIVLMFMLFIG
                     NLTILPIYMQTMMKWSPLESGLILLPGGLIMGLLSPVTGKLFDKIGGRILSIMGMLTI
                     MIGALLMAQFSQNTTQLYVIISFSVTMLGNAMIMTPMTTQALNALPRQYIAHGTAMNN
                     TIRQVSAAIGTGILVTLMTGLGKTSSLSGSQGLIHGLDITFYVVALIAFIGTIIAFFI
                     RKH"
     gene            3096..3299
                     /locus_tag="VB241_002415"
     CDS             3096..3299
                     /locus_tag="VB241_002415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245666.1"
                     /translation="MKNAIMKEVVYINFEGLEQYLNGDNTRYNTLSLETLNYYLKQYV
                     LTVPFKNMDVQNGMKVSAEVKDI"
     gene            3309..3674
                     /locus_tag="VB241_002416"
     CDS             3309..3674
                     /locus_tag="VB241_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEP1245667.1"
                     /translation="MNHQHGDFYYEMNNFFNVYFEYKGFTVNIVSSTIHTHGDGKSLK
                     GSHVSLIIPIDGVDYVTGADFGDLPFSVMPIVPKDNSPVIHDMNGDFHAIYVNDYLFY
                     VRKMGKDNDNNLDHTMKWN"
     gene            3949..4523
                     /locus_tag="VB241_002417"
                     /pseudo
     CDS             3949..4523
                     /locus_tag="VB241_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         1746 a          810 c          753 g         1604 t
ORIGIN      
        1 ttattcctta cctctataaa aaatgtctca aaaaacatat aaacaaatat aaccattcaa
       61 tcattaaagc ataaagaacg ctacttcatt tccattaata tttatataga aagtaaagta
      121 gcgttattct aacttattta gtttatacca taatataatg tctctagaat aaaacgctag
      181 tgattatggt tttcttcaat ttgataaaat tcaatcgcat tgcctcttaa tataccatta
      241 atcgtgtcag ggtcaatatt actgttttta atatagtcta cgccgcgtgt atatttttca
      301 tcttggaagt aagggaagtc tgaccccatc ataaatttat ccttaccaaa tgtctcaata
      361 gagttaatca atgaaggttc gtggaagttt gctgtatcat accaaaattg tcgatttaat
      421 acttcataag gatcaatatt aaaggcattc caatcttcat aattgtcttt aatacgagtc
      481 atgaagaatg gtaaagcgcc acctaaatgt gaaatatgga atttaatatt tggatatttt
      541 tgcggaattt catttttaat caattgtaat gtaataaagg tagactctaa cggtgcacca
      601 ataacccatt ctaattgata gtcattgact aacggacttt gagcgccaca acctgttgga
      661 tgaatataaa ttgtagcatt gagctcattc atcgctgcaa agaaaggttc aaattgttta
      721 tcagcaacag agaccttatc ttttactata gttggaatgg caatgcccac aaattcatct
      781 tttttcaaaa gggcttgggc ttcttcaata gcttgatcga catagggtag tgagacagca
      841 ccatatgcca agaaacgatc cggatattgt tgaactaatc tttcatataa gtcattaatt
      901 tcttgagcac tttggtgagc ttcttcttta gtaccccatt gtggagattg tggtgtcgca
      961 gaaataattt gtaaatctac gccagcatcg tccatcattt tgaagcgttt ctctaaatct
     1021 tctttagacg cagattgatt aatcccttta gcaacttcag taccttgact acctaatttg
     1081 ccaagtttgt ctaaatagtc ttcattccat aaatgtgcgt gtgtatcaat cgcttttgtt
     1141 tcagtcatat tatcccctcc attaaaaatt gtcattcata atttgcttat tgccaatagc
     1201 ttatttatta cgagttaagc ttaacacttc tagtaagctt aatacattta aaaagaaaaa
     1261 tttagaagca gcttgtgcac ctttataacc atgcccagct ttataatggt tataaatagc
     1321 tgtacccata acaatattaa tcataatcgt accaacaact actaattttt tccctaattt
     1381 accgaaaata gaagtaaata aaagaagtga accaatcatt tcgaaagcgc cagctaatct
     1441 cattgatcta cgagataaac caaagccttg ttcgaattgt tgagccattt catcactatt
     1501 ttgtactttt ggtaaactgc ttttaaaaat tttactacct acatatgtgt taatgacgtg
     1561 tcttaataac attttaaaat cctccaaata attaatgttt gcgaataaag aatgcaatga
     1621 tagtacctat aaaggcaatc aatgcgacga catagaatgt aatatctaga ccatgaataa
     1681 gtccttgact accacttaat gaagaggttt ttcctaatcc tgtcatcaat gtgaccaaga
     1741 taccagtacc tattgcagct gacacttgtc taatcgtatt attcatggct gtaccatgtg
     1801 caatgtattg acgtggcaaa gcatttaaag cttgagttgt cattggcgtc atgatcatag
     1861 cattacctaa catggtaact gaaaatgaaa taatcacata tagttgtgtt gtattttgac
     1921 taaattgagc cattaataat gcaccaatca taatcgttag catccccata atactcaaga
     1981 tacgtccacc aattttatcg aataatttac cagtgacagg cgataacagt cccataatta
     2041 acccaccagg taataaaata agtcctgact ctaacgggga ccatttcatc atcgtttgca
     2101 tataaattgg taagattgtt aaattcccta taaataacat gaacattagt acaattaaaa
     2161 tcattgccac tgaaaagtcg ttatatttaa atactctaaa ctctaacaat ggcgattcca
     2221 gttttaattg tcggaaaata aataccgtta atataactat ggcagcaata atagttatat
     2281 aaaccattgg atgtgaccaa cctaagtttc ctgctgtact aaaaccatat agtaagccac
     2341 caaagctaac cgtcgataaa ataacggata gtttgtctaa gtttggttta cttagttccg
     2401 ttatatttgg caatgataga tacccgaata caaaatcaat catacctatt agtaatacga
     2461 ctaggaacag atagcgccaa tcgaaaatat tgacgaacca accagctgca gttggtccaa
     2521 tcgcgggtgc aaaacctatt acaagaccga acattcccat tgccattcca cgtttttcag
     2581 gtggaaacat aatgaaaagg agtgtctgtg tgagtggcat cagtatacca gcacctaagg
     2641 cttgaatact acgtcctaat aataacattg gaaaattgat tcctagcata caaataaatg
     2701 aaccaatcat taaaaagcat gttgcattaa aaaataaaga acgtaatgta aaacgttcaa
     2761 ttaaaaatgc tgtgatagga atcataatcc cattgactag cataaaaatt gtcgtgagcc
     2821 attgtgctgt actacttgat accgtaaaat ctttcataat ttctggaagt atcgtagtca
     2881 tcaaagtttg attcagcaca ccaacaaaag caccaatcat tacgacagct ataacacggt
     2941 tccgttgttt caaatctata ttaaagcata tacattcctc aaattttgac gcctccctct
     3001 cttttaagtg gaacctacta tcatttgttt tatacggaat acgttccgtt tgtcaattta
     3061 ataactcaag ggtatgataa taggtatctg ataaaatgaa aaacgcaata atgaaggagg
     3121 tagtttatat aaattttgaa ggattagaac aatatttaaa tggtgataat acaagatata
     3181 acacgcttag tttagagacg cttaattatt atttaaaaca atatgtgtta actgtcccgt
     3241 tcaaaaatat ggatgttcaa aatgggatga aagtttctgc agaagtaaaa gatatttaaa
     3301 ataatgtagt gaaccatcaa cacggtgact tttattatga aatgaataat ttctttaacg
     3361 tgtattttga atataaagga tttactgtaa atatagtttc ttcaactatc catacccatg
     3421 gtgatggaaa aagcttaaaa ggttctcacg tgtctttaat catacctatt gatggcgttg
     3481 attatgttac aggtgctgac tttggagact tgccatttag tgtcatgcca attgttccaa
     3541 aagataatag tccagtcatt catgatatga acggtgattt ccatgcaatt tacgtcaacg
     3601 attatttatt ttatgttcga aaaatgggga aagataatga caataacttg gatcacacta
     3661 tgaagtggaa ttaacacctc gtcatattca tgactttgtt tacaatattg aatataatga
     3721 aattaatcct aatgctattt ttgtgaaacg ctagttagta acaatgatta aatctttttg
     3781 gtcgtgctac aatgttacaa aataatataa ctttaactaa gcaacaaaag aaagaataat
     3841 atgatgtaac acctgagaat gatagacaat ttttagaaga agcatttaat ttagatgtta
     3901 aaattgatag attagaaagt taaaaacaac gttaaaaaga ggaatataat ggaaaaaaat
     3961 caacgaaaaa aacgcagtga tgccgaatat aaccaacaga ttatcttaac tacaatggaa
     4021 gatttactag aacagggtga agatataagt actaagaaaa tgtcagatat tgctaaaata
     4081 tcaggtgttg gagttggtac attatatcgt cattttgaaa gtaaaacatt gttgtgtcag
     4141 gctattatgg ataaaaagtc gatcaaatgt ttatagaaat agaagatatt ttagcggaaa
     4201 atacacagtg gcccgtgcga gataaaatca atgttatatt aacaaagtat ctagatttaa
     4261 aagaagcaaa tttcacaaca ttaaatttta tagaaaaatc cagttcacac tctagttctg
     4321 taattaatat tccattcttt gagcgactaa aaaacttatt aattcaacaa ttcgaaaatg
     4381 ttcattccat agcagacttg gattttaaac ttaatctaat gctcaatgca ttttcttcag
     4441 atttctatta ttttgtaaaa cataaccaaa aattaacaaa acaacaattt ttagacaaat
     4501 tactagattt atatctaaaa taatatgttc tgaaaaatag aagtattttt tgaatcacta
     4561 aataacgcgc tattaaatgg cttcgctatg cccgtctaac acattagtgt aaaattttat
     4621 aaattgaatt tttgatgacg ggtcccttcc tagggtgctc tctcagacac cccaaccggc
     4681 acattattgt aagttgactt ttcgataata ttaaagattt aaaataataa agctattaat
     4741 tatgattagt ggtttcaaaa tgctattttt agaggtttaa gattgccatg aatttcgttt
     4801 tatttgttct catgttcagg tttctttata tcaactttta ttttagtaaa ccaaaaagag
     4861 tagcaacttc aaaattcgca tttatatcta aagataattt taaaatgcac atg
//
LOCUS       DATTQF010000164         4833 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.165, whole genome shotgun sequence.
ACCESSION   DATTQF010000164 DATTQF010000000
VERSION     DATTQF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.165"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            141..1292
                     /locus_tag="VB241_002418"
     CDS             141..1292
                     /locus_tag="VB241_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEP1245668.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETVLIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKINEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNLEFKTEQFKMSESRNLLKNRSQNYALIRLLNWLRTTN"
     gene            1457..2500
                     /gene="recA"
                     /locus_tag="VB241_002419"
     CDS             1457..2500
                     /gene="recA"
                     /locus_tag="VB241_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP1245669.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            2854..4413
                     /gene="rny"
                     /locus_tag="VB241_002420"
     CDS             2854..4413
                     /gene="rny"
                     /locus_tag="VB241_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP1245670.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT         1799 a          641 c          926 g         1467 t
ORIGIN      
        1 taatgatata ttgtaaaata atatttgttc attttttagg gaaatgtgtt gtagcatctg
       61 atacattgaa tttaaaattg atgtgaattt ttaaatggaa tacatgaaaa aatgaattaa
      121 acgatacaag ggggatataa atgtcaattg ccattattgc tgtaggctca gaactattgc
      181 taggtcaaat cgctaatacc aatggacaat ttctatctaa agtatttaat gaaattggac
      241 aaaatgtatt agaacataaa gttattggag ataataaaaa acgtttagaa tcaagtgttc
      301 gtcatgcgtt agaaaaatat gatactgtta ttttaacagg tggcttaggt cctacgaaag
      361 atgacttaac gaagcataca gtggcccaga ttgttggtaa agatttagtt attgatgagc
      421 cttctttaaa atatattgaa agctattttg aggaacaagg acaagaaatg acacctaata
      481 ataaacaaca ggctttagta attgaaggtt caactgtatt agcaaatcat catggcatgg
      541 ctccaggaat gatggtgaat tttgaaaaca aacaaattat tttattacca ggtccaccga
      601 aagaaatgca accaatggta aaaaatgaat tgttgtcaca ttttataaac cataatcgaa
      661 ttatacactc tgaactatta agatttgctg gaataggtga atctaaagta gaaacagtat
      721 taatagatct tatcgataaa cagactaatc ctacgattgc gcctttggca ggaagtcatg
      781 aagtatatat tagattgact gcaaatgccg actcaaaaga acaagcacaa tcattgattc
      841 aacctgttaa gcaagaaatt cttgatcgta ttggagaata ttattatggt tcagatgaca
      901 cattaattga gcaagctgta ataaagaaaa ttaatgaacc ttttgtaata tatgatggta
      961 ttactaatgg tgctttatat catcgattga aagaagtgga tttaaacgat gttctaaagg
     1021 gtatgattaa tcacaatgaa aactttgttg atattaataa acctattgag cagcaattaa
     1081 aagatgcagt gcaatttgtt aataaattat ttaatgtgtc atcagcaatt attctattag
     1141 agtatgatgg tgtagtccat ataggctatg ataataactt agaatttaaa actgagcaat
     1201 ttaaaatgtc tgaatctaga aatttattaa agaacagaag tcaaaattat gcgcttataa
     1261 ggttattaaa ttggcttaga acaacaaatt aattgtatta tcgataaaaa tataagcacg
     1321 tttgttcgtt tttgtgtttt gatttcaaga ttttacacga acaaatattc gcaaaacact
     1381 tgtattttat tttgaatcct tgtatagtat tggtaagata attaaaagat agcaatttca
     1441 attaggaggt ctcgctttgg ataacgatcg tcaaaaagct ttagatacag taattaaaaa
     1501 tatggagaaa tctttcggta aaggtgccgt aatgaagttg ggtgacaata taggtcgccg
     1561 agtttcaact acatcaactg gttcagttac attagataat gcgctaggtg taggtggcta
     1621 tcctaaagga cgaattattg aaatttatgg tcctgaaagt tctggtaaga caacagtagc
     1681 gcttcacgct attgctgaag tacaaagtaa tggcggggtg gcagcattta tcgatgctga
     1741 acatgcttta gatccagaat atgctcaagc attaggcgta gatatcgata atttatattt
     1801 atcgcaaccg gatcatggtg aacaaggtct tgaaatcgct gaagcatttg ttagaagtgg
     1861 tgcagttgat attgtagttg tagactcagt tgctgcttta acacctaaag ctgaaattga
     1921 aggagaaatg ggagacactc acgttggttt acaagctcgt ttaatgtcac aagcgttacg
     1981 taaactttca ggtgctattt ctaaatcaaa tacaactgct attttcatca accaaattcg
     2041 tgaaaaagtt ggtgttatgt tcggtaatcc agagactaca ccaggtggac gtgcattaaa
     2101 attctatagt tcagtaagac tagaagtacg tcgtgcagaa cagcttaaac aaggacaaga
     2161 aattgtaggt aatagaacta aaattaaagt cgttaaaaat aaagtggcac caccatttag
     2221 agtagctgaa gttgatatta tgtatggaca aggtatttct aaagagggtg aacttattga
     2281 tttaggtgtt gaaaacgaca tcgttgataa atcaggagca tggtattcat acaatggcga
     2341 acgaatgggt caaggtaagg aaaatgttaa aatgtacttg aaagaaaatc cacaaattaa
     2401 agaagaaatt gatcgtaaat tgagagaaaa attaggtata tctgacggtg atgttgaaga
     2461 aacggaagat gcaccaaagt cattatttga cgaagaatag tacacaaatt tatatctata
     2521 gttaaactta gcaaatatcc ttataggatt gattgaaagt gatattcatc tcataaagct
     2581 ggaataatat ctaactttat gggatgcact tcaaatcgag actataaggt tttttatttt
     2641 atttattttt acattatcaa tagttttata atcgagcttc aaaactttag aaaatagtag
     2701 aaatagcatt caatatagtg caaaagtgca aattgataac ttgacactta tctcctataa
     2761 accgtacaat taatttgtat gatttatata taatttcata aagtcatatt gaatttcata
     2821 taaagagcaa accctagaaa aggaggtgtt tgtgtgaatt tattaagcct cctactcatt
     2881 ttgctgggga tcattctagg agttgttgga gggtatgttg ttgcccgaaa tttgttgctt
     2941 caaaagcaat cacaagctag acaaactgcc gaagatattg taaatcaagc acataaagaa
     3001 gctgacaata tcaaaaaaga gaaattactt gaggcaaaag aagaaaacca aatcctaaga
     3061 gaacaaactg aagcagaact acgagaaaga cgtagcgaac ttcaaagaca agaaacccga
     3121 cttcttcaaa aagaagaaaa cttagagcgt aaatctgatc tattagataa aaaagatgag
     3181 attttagagc aaaaagaatc aaaaattgaa gaaaaacaac aacaagtaga tgcaaaagag
     3241 agtagtgttc aaacgttaat aatgaagcat gaacaagaat tagaacgcat ctccggtctc
     3301 actcaagaag aagctattaa tgagcaactt caaagagtag aggaagaact gtcacaagat
     3361 attgcagtac ttgttaaaga aaaagaaaaa gaagctaaag aaaaagttga taaaacagca
     3421 aaagaattat tagctacagc agtacaaaga ttagcagcag atcacacaag tgaatcaacg
     3481 gtatcagtag ttaacttacc taatgatgag atgaaaggtc gaatcattgg acgtgaagga
     3541 cgaaacatcc gtacacttga aactttaact ggcattgatt taattattga tgacacacca
     3601 gaagcagtta tattatctgg ttttgatcca ataagaagag aaattgctag aacagcactt
     3661 gttaacttag tatctgatgg acgtattcat ccaggtagaa ttgaagatat ggtcgaaaaa
     3721 gctagaaaag aagtagacga tattataaga gaagcaggtg aacaagctac atttgaagtg
     3781 aacgcacata atatgcatcc tgacttagta aaaattgtag ggcgtttaaa ctatcgtaca
     3841 agttacggtc aaaatgtact taaacattca attgaagttg cgcatcttgc tagtatgtta
     3901 gctgctgagc taggcgaaga tgagacatta gcgaaacgag ctggactttt acatgatgtt
     3961 ggtaaagcaa ttgatcatga agtagaaggt agtcatgttg aaatcggtgt agaattagcg
     4021 aaaaaatatg gtgaaaatga aacagttatt aatgcaatcc attctcacca tggtgatgtt
     4081 gaacctacat ctattatatc tatccttgtt gctgctgcag atgcattgtc tgcggctcgt
     4141 ccaggtgcaa gaaaagaaac attagagaat tatattcgtc gattagaacg tttagaaacg
     4201 ttatcagaaa gttatgatgg tgtagaaaaa gcatttgcga ttcaggcagg tagagaaatc
     4261 cgagtgattg tatctcctga agaaattgat gatttaaaat cttatcgatt ggctagagat
     4321 attaaaaatc agattgaaga tgaattacaa tatcctggtc atatcaaggt gacagttgtt
     4381 cgagagacta gagcagtaga atatgcgaaa taatttttgt ctccctcaca aattagtgag
     4441 ggagcttttt taagttgtag tttcaatcta gttagacagt actttattgg taataactat
     4501 attagacagt agttatttga aagtaagatg gaccttatat taaataagaa gttattgctt
     4561 ttaataaaaa tgttttaggt tcataattac tatatttata ttatgtaaac ctataaaggt
     4621 gattgctttt tcaaactaaa aaaaagaaga gaagatgtaa cacatcttct cttctgtaat
     4681 attaattaga aattatttct aagttgagtt attttaattg taaatctgtt ttctttaatt
     4741 cttttataac ttctgcagta tcataacaat ttgttgcaat tgttgaatat ctctctgcta
     4801 aacgatatgc attaatgtaa agctttaaac ttt
//
LOCUS       DATTQF010000165         4780 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.167, whole genome shotgun sequence.
ACCESSION   DATTQF010000165 DATTQF010000000
VERSION     DATTQF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.167"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..161
                     /locus_tag="VB241_002421"
     CDS             <1..161
                     /locus_tag="VB241_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP1245671.1"
                     /translation="IYVGDAVFDIKMAKNANIDSCAVTWGSHSIEVLNTENPNFIINN
                     VKELNFEE"
     gene            165..1313
                     /locus_tag="VB241_002422"
     CDS             165..1313
                     /locus_tag="VB241_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245672.1"
                     /translation="MSNIKIVTLIGFFTMFIVGVSDLIVVGMLNEMSKEFHVSNALIG
                     QLVTLYAVAFSILSPLLTKVTSQINDKKLLVSCLILFIIFSMLTTVMTNYFFLCIVRI
                     FMAGIASLITVKLMSTGANIVPVEYKASVIANIYVGFSAANILGIPIGTLIASHYNWK
                     IPFYIIAIITFLCLLGIMLFIPNKSHYSINNHSKYQLVNFRGTLIMLGFLLIMMISNS
                     MLFTYIEPLVKSKGHSIWIVSLCLFFSGIAGVLGSKLGNNLTEKKGYRIAGNIIIIVY
                     IISIMSILLTGQNVILLILSIFTWNLFHWGTNPTVQYALLKFLKGDPSQILSYDISIL
                     NLGIGLGSLIGGILFSIDNQFNLSIVVAMILAMVSSILLKTAVHTEKN"
     gene            complement(1399..3441)
                     /locus_tag="VB241_002423"
     CDS             complement(1399..3441)
                     /locus_tag="VB241_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEP1245673.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(3781..4218)
                     /gene="mnhG2"
                     /locus_tag="VB241_002424"
     CDS             complement(3781..4218)
                     /gene="mnhG2"
                     /locus_tag="VB241_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HEP1245674.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSTKALQLRAKKREEYRKKWYQND"
     gene            complement(4193..4495)
                     /gene="mnhF2"
                     /locus_tag="VB241_002425"
     CDS             complement(4193..4495)
                     /gene="mnhF2"
                     /locus_tag="VB241_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HEP1245675.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGYVFNGNNKRNL"
     gene            complement(4492..>4780)
                     /gene="mnhE2"
                     /locus_tag="VB241_002426"
     CDS             complement(4492..>4780)
                     /gene="mnhE2"
                     /locus_tag="VB241_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HEP1245676.1"
                     /translation="LYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDWAITFLTI
                     LIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKNYEDLILEVSR"
BASE COUNT         1679 a          774 c          668 g         1659 t
ORIGIN      
        1 ctatatatgt tggagatgct gtttttgata ttaaaatggc taaaaatgcc aatattgatt
       61 cttgtgctgt aacttgggga agtcatagta ttgaagtatt aaacactgaa aatccaaatt
      121 ttataattaa taatgttaaa gaacttaatt ttgaggagta agatatgtca aatattaaaa
      181 ttgtaacttt gattggcttt ttcacaatgt ttattgttgg tgtttcagac ttaattgttg
      241 taggaatgtt aaatgaaatg agtaaagaat ttcatgtaag taatgcttta ataggtcagt
      301 tggtaacatt gtatgctgtt gctttttcaa ttctaagccc tctcttaaca aaggtaacta
      361 gtcaaattaa tgacaaaaaa ttattagtat cctgtttaat actttttatt attttcagca
      421 tgcttacaac tgtaatgacc aattatttct ttctatgtat tgttagaatc tttatggcgg
      481 gcatcgcttc attaatcact gtaaaattaa tgtctactgg agcaaatatt gttccagtag
      541 aatacaaagc atcagttatc gctaacatat acgtaggctt tagtgcagca aatatacttg
      601 gtatacccat aggtacatta attgcttccc attacaattg gaagattccg ttttatatca
      661 ttgcgattat cactttttta tgtctactag gtattatgtt atttatcccg aataaatcac
      721 attatagtat taataatcat tctaaatacc aattagtaaa tttcagaggc acattaatca
      781 tgttgggctt tttattaatc atgatgatat ctaatagcat gttatttaca tatatagagc
      841 ctttagtaaa aagtaaaggt catagcatct ggatagtaag tttgtgttta tttttctcag
      901 gtattgcagg tgttttaggt tcaaaattag gaaataattt aactgaaaag aaaggctata
      961 ggattgctgg aaatatcatt ataattgtct atattatttc tattatgagc atcttattga
     1021 ctggacaaaa tgtcatactt ttaattttat ctatatttac atggaattta tttcattggg
     1081 gaacaaatcc aacagttcaa tatgctttac ttaaattttt aaaaggcgat ccaagtcaaa
     1141 tattgagcta tgatatttca atattaaatt taggaatcgg attaggatca ttaattggtg
     1201 gtatattatt ttctatcgat aatcagttca acttatcaat tgttgttgct atgatattag
     1261 caatggtttc atctatatta ttaaaaacag ctgttcatac agagaaaaat taaaactatc
     1321 tattcaattc ccctaaataa aagggattga tgaaaagcca ttcagttttt catcaatccc
     1381 ttttagcata attacgaact acgtcatact atctacaata accatttcat cataattgat
     1441 ggcttctctt agttttaacg ctgtaccttc tgatacttca tctgtatcaa tcaactcacg
     1501 taaaatgcga cgttgttctc tcaatgcatt taatttaatt ttagtaatcg tatcttcact
     1561 tgctgagttg aagaaattag atggtgttaa gttttcaatt cgcattaaat aaccgtcaca
     1621 aaccattcca acttcaagtg tattatcttt tgttgtttcc ttagacaaac gactaaccac
     1681 attataatgg actaatttat taattttatt taattccatt aagttatccg taacactaag
     1741 tgaagatgcc gagttcactc tcgtttgtac tcttcgtttt aataaagcac ctcgtaataa
     1801 tacaatcatt cttcttatta atgatgcttg tctatatact tgtgttcttt cagcataacg
     1861 catatagttt tcaagtacac tattcgttat ttgtccttca tctactaaag actctaaagt
     1921 ctttgtttct acattaaaag caattttttg tagacgttct aattctttag agttttcatc
     1981 atctttctct acagttttta aaaatgctaa tttatcatga tattctttaa tcacattacc
     2041 atatttaaaa cttgtttcga aagtagattt ttgatttaga taatcaataa cttgttctaa
     2101 tatataaatt ctagcaactt taaacgacat attgccaatt actgttttag gtgcaggttt
     2161 cgttaataat ggcaataata cttgcgcaac taccaaacta ataataacca taccagatgc
     2221 aataaataat aaatcatttc tatacgtaaa agcatgatga cctgctaaaa aatacggtaa
     2281 cgttaatgcg attgctaaag aaattgttcc atgcacacca cataacgtca taattaaagc
     2341 gtataaactt cgctttggtg gtttctcagt cgttggatta tcatcatcat ttttagtcat
     2401 cattttttgg aatggactga tggctaaata aaaataagga tataagacat aaacccaaac
     2461 aaatctaaat agatagacag ctaaagcaac aacaatagtg atgcctatta aaaagattaa
     2521 attgtgcggt tctgttttga taattttaat aataacttca ggtactaaaa atcctagtat
     2581 tgaaaaaaca aagccattta aaacataacc tagtatattc catgtatgat tgtaactcat
     2641 ttgtagttgt gtacgtactt gcataattct gtcacgttcg aaaccatgta caagtcctgc
     2701 aactactgct gcaatgattc ctgatgcgtg aaacaattca gcaattaaat acgtaacaaa
     2761 tggtgttaac aattgaataa ttgtaaacat attaatgttt tcatatcctc gacgcatcaa
     2821 tgttaatcgg aaccttacta atgccatacc tataagtaaa ccaaccactg cgccaccaat
     2881 tgatgcaatt aaaaacaact gaacagcatc aacaagtgaa aaagcacctg taactaatac
     2941 tccaacagct attttaaatg aaataatacc agcagcatca ttcaataatg actcaccttc
     3001 aagaattgtc attgctcctt ttggcaagac ctttccttta gtaattgctt gcactgctac
     3061 tgcatcagta ggacaaagaa tggcagcaat tgcaaatgct gctccaatag gtaaatctgg
     3121 ccaaatccaa tgaataaata aaccgacacc tatcacagta gtaatgacta atcctaatgc
     3181 catcatcatc actggcttaa tatatttcct taaatggact ctagaaacat taacaccttc
     3241 tacaaataac aaaggcgcaa tcattgttac cataaataat tcagaatcaa aattaaattg
     3301 aacagggatt ggggtaataa atagtaacat gcccaagaaa atttgtataa atgctagggg
     3361 tactttaggt atgaaagtat ggacaaacga acttagtatc acaacagcta taaatataag
     3421 aattgtttca aatatttcca aactttcacc tctctaaaaa gtattgttta attgaaaaat
     3481 aagtatcaca tctcgttgta attatacttt agaggataaa ttgagttagc gaccacaaaa
     3541 gcactttaat atagatatat gtctacgatt gcagtactta aatttgcaat tatttaattt
     3601 tattttatca ctaattgttt gtataaataa acaacttgct ttcacataac aacattaact
     3661 tataatacaa aaaatgagca ccttaaaatc gactaaccaa tttcaaagta ctcttttaat
     3721 gattaatttt gaaaacaaat cttcaaaagt attgttatgc ttaacaattt agccaatact
     3781 tcaatcgttt tgataccatt tcttacgata ctcttctcgt tttttagcac gtaattgtaa
     3841 tgcttttgta gagttttgtt catttgaact taataatatt gatgcatgtg tgtgatcatc
     3901 attttttcga tacatataag cgccgttgcg ataagcagcg cgagcgacta agtgcatgcc
     3961 gactggtgaa gttaaattaa taaaaacaag tgacagtaat aaacgcacac tgaaaaatcc
     4021 tgtattcaca ataaaataaa ttaatacacc aattaaagtt aataacacgg ataaagtcga
     4081 actttttgtt gcagcatgac ttcttaagaa aacatcttgg aatttcacga tacctattgc
     4141 actaataaga gcaataaaac tacctaacaa caacatcaca gcagcaataa gactaaagat
     4201 ttcttttgtt atttccatta aacacatacc ccccaccaat aaatcttgaa atcgatacag
     4261 agcttacaaa agatataatg gcaatgagca tgattgaatc taggaaagaa acggtgccca
     4321 ttagtacact taagacaccc acaatagaca tgacaacagc acttgttgta tcaaatgtaa
     4381 cgacacgatc tgctgttgta ggtcccttga ttaatctaaa taaacagatg attaatgcaa
     4441 ttccaaaaat aatgagtgaa ctaataatca taatatgtgt gattgtttgt atcatcgcga
     4501 cacctccaat attaagtctt cataattctt aatacttctt aacaaactat ctttttcttt
     4561 ttcagacacg tcgatactat gaataaaaaa cttttttgag tcttgagaaa ttcgtattac
     4621 tgtagaccct ggagttataa taattaaaat tgttaaaaat gttattgccc agtcacttgt
     4681 tagtcttgtt tcataagaaa gtaatccagg gttcatatct tttgttttaa aaagaatata
     4741 attaatcgtg ctaatgctag atgttattaa ttgatataaa
//
LOCUS       DATTQF010000166         4780 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.168, whole genome shotgun sequence.
ACCESSION   DATTQF010000166 DATTQF010000000
VERSION     DATTQF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.168"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            177..670
                     /locus_tag="VB241_002427"
                     /pseudo
     CDS             177..670
                     /locus_tag="VB241_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            780..1574
                     /locus_tag="VB241_002428"
     CDS             780..1574
                     /locus_tag="VB241_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245677.1"
                     /translation="MIQSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDGFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENLKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSKNIEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(1578..1700)
                     /locus_tag="VB241_002429"
     CDS             complement(1578..1700)
                     /locus_tag="VB241_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245678.1"
                     /translation="MSYIIKFTFKFFEMTTYISQLTLDIELVSSELLVILSGFI"
     gene            2135..2326
                     /locus_tag="VB241_002430"
     CDS             2135..2326
                     /locus_tag="VB241_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245679.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(2465..2648)
                     /locus_tag="VB241_002431"
                     /pseudo
     CDS             complement(2465..2648)
                     /locus_tag="VB241_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2584..2865)
                     /locus_tag="VB241_002432"
     CDS             complement(2584..2865)
                     /locus_tag="VB241_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP1245680.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEIKKLDDVRMPFSSAHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            3164..3859
                     /locus_tag="VB241_002433"
     CDS             3164..3859
                     /locus_tag="VB241_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP1245681.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKMAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAKAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITATYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            4111..4533
                     /locus_tag="VB241_002434"
     CDS             4111..4533
                     /locus_tag="VB241_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1245682.1"
                     /translation="MTKKHVFIIIGVILCICIVATVIYLKVKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKTGPMGDAVIEGYINENKKADFVAYGSPEHHYQ
                     FGGSLIKSKNLSTLLKPAHQTKSPDEIKKELESKKNDR"
BASE COUNT         1845 a          678 c          812 g         1445 t
ORIGIN      
        1 gtcgaagcgc ccaaattaaa gacacattta atcaaacatt aaaattatat ccaaccaaaa
       61 atctcgatga tttttacgat aaagaaggct ttcgagatca agaatttgac aaaggagaca
      121 aaggtacatg gataatcagc tctgaaatga atattcaatt aaagggagaa agtttaaaat
      181 caagaggaat gattctatat atgaatagaa atacaaaaag gacaacaggt tatttttttg
      241 taagtgaaac aaaaggaaaa acgtatgaaa aagaaaaaaa tatccagtca aaatggaaca
      301 taatcaaatt atcccaacga agccaataca agatgacaag ttgaaaaaag aaattgaaaa
      361 ctttaaattt tttgtacaat atggcaattt taaagatatt aatgattata aaaatggtga
      421 tatttcatat aatcctaatg tgccaagtta ttccgaaaaa tatcaattga ggaatgatga
      481 ttataatgtg caacagttaa gaaaaagata tgacatacca acgaaacaag caccgaaaat
      541 gttattaaaa ggcgatggtg atttaaaagg ctcatctgta gggtctaaag agttagaatt
      601 tacttttgta gaaaataaaa aagaaaacat ctatttttct gatagtatta atttcaaacc
      661 tactgaatag agttaataga aatttggaat gaaaattcca ttaagtgtta aaaaattttt
      721 ctctttagat gtataaatta aagattgagt cagtattaaa aatataaggt ggagaaatga
      781 tgattcaatc aaagaaatta acgctcggta tatgcttggt attactcatt atattgattg
      841 taggttatgt cattatgaca aaaacaaatg gtcgaaacgc ccaaattaaa gacacattta
      901 atcaaacatt aaaattatat ccaaccaaaa atctcgatgg tttttacgat aaagaaggct
      961 ttcgagatca agaatttaaa aagggtgata aaggtacttg gatagttaat tctgaaatgg
     1021 taatcgagcc aaaaggtaag gatatggaaa cgagaggaat ggtgctctat atcaatcgca
     1081 atactagaac cacaaaaggg tattatttta taagtgaaat gacagatgac agtaacggca
     1141 gaccaaagga tgatgaaaaa aggtatccgg taaaaatgga acataataaa atcataccaa
     1201 cgaagccact accgaatgac aagttaaaaa aagagattga aaaccttaag ttctttgtac
     1261 aatatggcaa ctttaaagat attaatgatt ataaagatgg tgatatttca tataatccta
     1321 atgtaccaag ttattcggca aaatatcaat tgaataatga tgattataat gtccaacagt
     1381 taagaaaaag atatgatatt ccaaccaaac aagcgccgaa actattattg aaaggtgatg
     1441 gtgatttaaa aggatcatca gtaggatcaa aaaatataga atttaccttt gtagaaaata
     1501 aagaagaaaa catctatttt acggattctg tacaatatac tccaagtgag gatacaaggt
     1561 atgagtcaaa ctgaatatca aataaaacct gataatataa caagtaactc tgaagaaaca
     1621 agttcgatat ctaaagtgag ctgtgaaata taggtagtca tttcaaaaaa tttaaaggtg
     1681 aatttgataa tgtagctcaa ggagattggg ttaaaaaggc gaagaatgaa ctggatgata
     1741 ttagtaagaa attaaaaaat attcaaagaa cggaagttta atattctagc cgattcctga
     1801 aactaagaca gcgtgtgttc attcatgcca ttatcaatat aagcaccaca acaaaaaagc
     1861 ttctaatgtg atacaggaac ctcatattcc gtatcatgtt agaagctttt aatgtctaaa
     1921 gaacatctac attttatcat attttctgac ttattaaact tttatataat taactatttc
     1981 ttaattttcc aaaatagtga taaatttgtg aaatgcatca caaacccctt tatttattta
     2041 gaaattcatg taatattaga cttgtaagaa gttaataaat agagagagac gagagagttt
     2101 atataaataa tatataaaca ttggagtgat gattatgaga aaagagattg aagcgcttat
     2161 tttctcagac gtaagtagct atgatattta cgtgaacact ggtgtaaatc aaggattaat
     2221 tggtgacatc aaagatggtt acctaactat tgattctatg ccttacattg atgctgagcg
     2281 tttgtatcac tttgctatgg aacgtaaatc gttagtcact aactagttct tattgccaat
     2341 gattactacc cctagtcggc ggcaattgaa gtgtgattga tgtaacttgc cctcgttggt
     2401 gagcaattga gggcagaccc ctttaattaa gtaaacccta actccccaca aatctggaac
     2461 gatactaaaa gccacgtcct atattggatg tggctttagt catgtctata ttgaatttta
     2521 gtatgattac ctacaagatt atatataact gtatcatgtc cacgatatat aaacgaaatg
     2581 atattagaat ccttcatttt caacttgttt gaaattacgt ttaatgccat tgtatgaata
     2641 cacaacatta tacagtttca cttgggcatc atgtgcactt gagaagggca ttcttacatc
     2701 atctaatttt ttgatttcgt gtcgaaatgc tttctcggct ttttgtcttt ctgcaagcga
     2761 catacttagc cattcttctt tgctaaataa atcctcgaat ctaaaatcaa atgcagttct
     2821 tttactttct gctctcagtg ctaacacacg ctcatgatat gtcacaataa ctcccccatt
     2881 tctaacagcc ttgccattgt ttactaatac ttataacttt agtactaact ttcgactttt
     2941 acaataaaga attgacaatt tttcaacatt tttaaaaata aaaaatatga acaacgatta
     3001 tttgtatatt ttgaataact taaaaatgta ctttatcagt gtaaataaat cgagttagta
     3061 taaccagaat atgaattgtg atacgctttt taaaagtata aatagtaata tgtacttatg
     3121 atgtacccta aaaaaataaa aagaatcaaa ggagtcgatc attatgacag tattaacaga
     3181 taaaatagca gtagttacag gtgcaggtag tggtattgga gaagcaattg caacattact
     3241 acatgaagaa ggggcgaaag ttgtcttagc cggtagaaat aaagataaac tacaaaatgt
     3301 ggcgaatcaa ttagctcaag atagtgtgaa ggtagtgcca actgatgtaa cgaaaaaaga
     3361 agaagtcgat gaattgataa aaatggcaca acaaacattc ggtggtttgg atattgtcat
     3421 caatagtgcg gggcaaatgt tgtcgtctaa gattactgat tatcaagtag atgaatggga
     3481 tagtatgatt gatgttaata tcaaaggcac tttatacacg gcaaaggctg cattaccaac
     3541 tatgttagaa caatcaagtg gccatcttat taacattgca tctatttctg gctttgaagt
     3601 aacgaaaagc agtacgattt atagtgcgac gaaagcggca gttcacacta ttactcaagg
     3661 attagaaaaa gagttggcaa agacaggtgt taaagtaaca agcatttctc caggaatggt
     3721 tgatacagca ataactgcca catacaatcc aacagatcgt aaaaaacttg agccacaaga
     3781 tattgcagaa gcagtattat atgcattaac acaaccaaag cacgttaacg tgaatgaaat
     3841 tacagtgcgt cctgtataaa aaggtgtttg aggtgtggga ggtaggcatt taatgtaata
     3901 gctatcggtt ttgttaatat tagtaaggca ataatgatat aaaagcatga taggaaatta
     3961 ttccttgcac aattctcagt aactggtgat tttaacaatc gagtgagtga aggcgacgat
     4021 attagtgcta tgatttataa tctataatgt tcataattac agaactataa aaactttttc
     4081 attttccacc acattttagg agttgcttta atgaccaaaa aacatgtttt tataattatt
     4141 ggtgttattt tatgtatatg tatagttgct acagtcattt atttaaaagt gaaatatgac
     4201 gaaaaagaaa agcaaaaatc aatttattat aaagaacaac aagagcgtat tacactctat
     4261 ctcaagcata acactaaaga accgaacacg atcaaatctg tacatttcac aagtttaaag
     4321 acaggaccta tgggcgatgc cgtaattgaa ggttacatca atgaaaacaa aaaagctgat
     4381 tttgttgcat atgggtctcc agaacatcat tatcaatttg gtggaagttt gatcaaaagt
     4441 aaaaatttaa gcacgttatt aaaaccagca catcaaacca aatcacctga tgagataaaa
     4501 aaggaattag agtctaaaaa gaacgaccgc taaaactatg cagtcgtttt aattttgctt
     4561 tcaagttaat catacttaca cataaaaatg aaaagttgaa tttttagtac aatttccact
     4621 gaatttatct gttaaccact tcataaccgc aatattccga tgatcttaga tgaactttat
     4681 atcaacactt ttaattgctt ttccttgacc tagattaaaa ttatagtttt tctttgtaag
     4741 agcgtgttat gtagtatcat aaacatgtaa actgtataaa
//
LOCUS       DATTQF010000167         4734 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.169, whole genome shotgun sequence.
ACCESSION   DATTQF010000167 DATTQF010000000
VERSION     DATTQF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.169"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            50..1717
                     /locus_tag="VB241_002435"
     CDS             50..1717
                     /locus_tag="VB241_002435"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP1245683.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(1963..2052)
                     /locus_tag="VB241_002436"
     CDS             complement(1963..2052)
                     /locus_tag="VB241_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245684.1"
                     /translation="MLLTTPIRWYLIDNNYQYQLLVLIVAHPN"
     gene            2051..4723
                     /gene="harA"
                     /locus_tag="VB241_002437"
                     /pseudo
     CDS             2051..4723
                     /gene="harA"
                     /locus_tag="VB241_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
BASE COUNT         1821 a          741 c          884 g         1288 t
ORIGIN      
        1 aacttacata ttataaatgc ataaattaaa caaaaagggg cgaaaaaagt tgactcattt
       61 atcagattta gatattgcga atcaatcaac actacaacca attaaggata ttgctgcatc
      121 agtaggtatt tcagaggatg cattagaacc ttatggtcat tacaaagcta aaatcgacat
      181 taataaaatt acgccaagag aaaacaaagg gaaagttgtt ttagtaactg cgatgagccc
      241 aacaccagct ggtgaaggta aatcaacggt tacagttggt ttagctgatg cattccatga
      301 gttaaataaa aacgttatgg ttgcattaag agagcctgct ttaggaccaa catttggtat
      361 taaaggtggt gcaactggtg gtggttatgc gcaagtctta cctatggaag atatcaactt
      421 acatttcaac ggagatttcc atgcgattac aactgctaat aatgcattgt ctgcgtttat
      481 cgataatcat attcaccaag gtaatgaatt aggaatcgat caaagacgta ttgaatggaa
      541 acgtgtatta gatatgaatg atcgtgcact tagacatgta aacgttgggt taggtggacc
      601 tacaaatggt gtaccacgtg aagatggctt taatattaca gtagcgtctg aaattatggc
      661 gattttatgc ttaagtagaa gtattaaaga cttaaaagat aaaattagtc gtattactat
      721 tggttacact agagatcgca agccagttac agttgcagat ttaaaagtgc aaggtgcact
      781 tgcaatgatt ttaaaagatg caattaaacc aaacttagta caatcaattg aagggacacc
      841 agcattagtt catggtggac catttgcgaa tatcgcacac ggttgtaact caattttagc
      901 aactgaaaca gcacgtgatt tagctgatat cgttgtaacg gaagctggat ttggttcaga
      961 cttaggcgct gaaaaattca tggacattaa ggcgcgtgaa gctggattcg acccagcagc
     1021 agttgtagtt gtagcaacga ttcgtgcatt aaaaatgcat ggtggtgtag caaaagataa
     1081 tttaaaagaa gaaaatgtag aagcagtaaa agcaggaatt gttaatttag agcgtcatgt
     1141 taataatatt aaaaaattcg gtgtagaacc ggttgttgca attaatgcat ttatacatga
     1201 taccgatgca gaagtagaat atgtaaaatc ttgggctaaa gaaaataacg tacgaattgc
     1261 cttaactgaa gtttgggaaa aaggtggtaa aggtggcgtt gacttagcaa atgaagtatt
     1321 agaagtcatt gatcaaccta attcatttaa acctttatat gaattagaat taccattaga
     1381 gcaaaagatt gaaaagattg tgactgaaat ctatggcggt tcaaaagtaa cgtttagtag
     1441 taaagcgcaa aaacaattaa aacaatttaa agaaaatggt tgggataatt acccagtatg
     1501 tatggcgaaa acacaatatt cattctcaga tgatcaaacg ttgttaggtg caccatcagg
     1561 atttgaaatt acaattcgtg aattagaagc gaaaacaggt gcaggattta tcgtagcgtt
     1621 gacaggtgca atcatgacta tgcctggttt acctaaaaaa ccagcagcat taaacatgga
     1681 tgttactgat gatggtcatg caattgggtt attctaatac atcatgtcaa ttgtttaata
     1741 aagataagta aatagtttaa tagaccggac tgttggagat gcattatttc agcagttcgg
     1801 tttttttgct gtgctaaaaa tagattcaat ttggcgaatc taacggcaat gtttgaagat
     1861 ggttaattaa tgtattccaa gataaaaagt gggcttgaag aataggaaag cgatgcaatg
     1921 aacattcata ttaaaaattt ttaataaaat aggttgcaat atttaattgg gatgcgctac
     1981 aattaacact aataattgat attgataatt attatcaatt aaataccatc ttataggagt
     2041 tgttaacaac atgaacaaac atcacccaaa attaaggtct ttctattcta ttagaaaatc
     2101 aactctaggc gttgcatcgg tcattgtcag tacactattt ttaattactt ctcaacatca
     2161 agcgcaagca gcagaaaata caaatacttc agataaaatc tcggaaaatc aaaataataa
     2221 tgcaactaca actcagccac caaaggatac aaatcaaaca caacctgcta cgcaaccaac
     2281 aaacactgcg aaaacctatc ctgcagcgga tgaatcactt aaagatgcaa ttaaaaatcc
     2341 tgcagtagaa aataaagaac atgatatagg tccaagagaa caagttaatt ttcaattatt
     2401 agataaaaac aatgaaacgc agtactatca ctttttcagc atcaaagatc cagcagatgt
     2461 atattacact aaaaagaaag ctgaggttga attagacatc aatactgctt caacatggaa
     2521 gaagtttgaa gtttatgaaa acaatcaaaa attgccagtg agacttgtat cgtatagtcc
     2581 tgttccagaa gaccatgcct atattcgatt ccctgtttca gatggtacac aagagctgaa
     2641 aattgtctct tcaactcaaa ttgatgatgg agctgaaaca aattatgatt atactaaatt
     2701 agtatttgct aaacctattt ataacgatcc ttcacttgta aaatcagata caaatgatgc
     2761 agtagcaacg aatgatcaat caagttcaga cgcaagtaat caaacaaaca cgaatacatc
     2821 taatcaaaat acatcaacga ccaacaatac taatgatcaa ccgcaggcaa cgaacaatat
     2881 gagttaacct gcacaaccaa aagcgtcagc gaattcagat caagcatcaa gccaaccagc
     2941 tcatgaaaca aattctaatg acaatactaa cgatgcaacg aatgcatcaa gtaatcagtc
     3001 aaatgggtac caacagtatc caccagcaga tgaatcacta caagatgcca ttaaaaaccc
     3061 ggctatcatc gataaagaac atacagctga tcattggcga ccaattgatt ttcaaatgaa
     3121 aaatgataaa ggtgagagac agttttatca ttatgctagt actgttgaac cagcaactgt
     3181 tatttttaca aaaacaggac caataattga attaggttta aagacagctt caacatggaa
     3241 gaaatttgaa gtttatgaag gtgacaaaaa gttaccagtc gaattagtat catatgattc
     3301 tgataaagat tatgcctata ttcgttttcc agtttctaat ggggcgagag aagttaaaat
     3361 tgtgtcatct attgaatatg gtgagaactt ccatgaagat tatgattata cattaatggt
     3421 ctttgcacag cctattacta ataacccaga cgactatgtg gatgaggaaa catacaattt
     3481 acaaaaatta ttagctccgt atcacaaagc taaaacatta gaaagacaag tttatgaatt
     3541 agaaaaatta caagataaat tgccggaaaa atataaagca gaatataaaa agaaattaga
     3601 tcaaactaga gtagagttag ccgatcaagt taagtctgca gtgacagaat ttgaaaatgt
     3661 tacacctaca aatgatcaat taacagatgt acaagaggct cattttgttg tttttgaaag
     3721 tgaagaaaat agtgagtcag tcatggatgg atttgttgaa catccattct atacagcaac
     3781 tttaaatggt caaaaatatg tagtgatgaa aacaaaggat gacagttact ggaaagattt
     3841 aattgtagaa ggtaaacgtg tcactactgt ttctaaagat cctaaaaata attctagaac
     3901 gctgattttc ccatatgtac ctgacaaagc aatttataat gcgattgtta aagtcgttgt
     3961 ggcaaacatt ggttatgaag gtcaatatca tgtcagaatt gtaaatcagg atatcaagac
     4021 aaaagatgat gacacatcac aaaataacac gattgaacct ctaaatgtac aaacaggaca
     4081 agaagataag gttactgcaa cggatacagc tgaaaatagc agcactgcaa caaatccaaa
     4141 agatgcgtct gataaagcag atgtgataga accagagtct gacgtggtta aagatgctga
     4201 tagtagtgtt gacaaagatg tgcaaaatga tgttgatcat ttatccgata tgtcggataa
     4261 cactcacctc gataagtatg atttcaaaga aatggatact caaattgcta aagacactga
     4321 taagggtgtt gataatagcc ttggaatgac atctaatgtc gatactgaca aagactctaa
     4381 taaaaatgaa ggcaaagtta tacaactagc acataatacc gataaaaatc atactggaaa
     4441 agcagcaaag cttgatggcg tgagacaaaa ttataataat acagacaaag ttactgataa
     4501 aaaaacaact gaacatctgc cgagtgatat tcataaaact gtagacaaaa cagtgaaaac
     4561 aaaagaaaag gccggcacac catcgaaaga aaacaaactt agtcaatcta aaatgctacc
     4621 aaaaactgga gaaacaactt caagccaatc atggtggggt ttatatgcgt tattaggtat
     4681 gttagcttta ttcattccta aattcagaaa agaatctaag taattaactg gata
//
LOCUS       DATTQF010000168         4550 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.170, whole genome shotgun sequence.
ACCESSION   DATTQF010000168 DATTQF010000000
VERSION     DATTQF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.170"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..435
                     /gene="tilS"
                     /locus_tag="VB241_002438"
     CDS             <1..435
                     /gene="tilS"
                     /locus_tag="VB241_002438"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP1245685.1"
                     /translation="FKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQT
                     IEKPGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKV
                     QQWVRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            440..979
                     /gene="hpt"
                     /locus_tag="VB241_002439"
     CDS             440..979
                     /gene="hpt"
                     /locus_tag="VB241_002439"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEP1245686.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            1237..3330
                     /gene="ftsH"
                     /locus_tag="VB241_002440"
     CDS             1237..3330
                     /gene="ftsH"
                     /locus_tag="VB241_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP1245687.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            3744..>4550
                     /gene="hslO"
                     /locus_tag="VB241_002441"
     CDS             3744..>4550
                     /gene="hslO"
                     /locus_tag="VB241_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP1245688.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEA"
BASE COUNT         1663 a          638 c          912 g         1337 t
ORIGIN      
        1 tttaaacaat ttagtagtaa gaaagcacaa ttcagtatta atctcacgga taaatggata
       61 attcaaatcg catatggtaa attaataata atggctaaaa ataatggcga tacatatttt
      121 agagttcaaa ctattgaaaa gccaggtaat tatattttta acaaatatcg attagagata
      181 cattctaatt taccaaaatg tttatttcca cttacagtga gaacacgaca aagtggcgat
      241 acatttaaac tgaatgggcg cgatggttat aagaaagtaa atcgcctgtt tatagattgt
      301 aaagtgcaac agtgggttcg ggatcaaatg ccaatcgtat tggataaaca acagcgcatt
      361 attgcggtag gagatttata tcaacaacaa acaataaaac aatggattat aattagtaaa
      421 aatggagatg aatagcgtta tgcataatga tttgaaagaa gtattgttaa ctgaagaaga
      481 tattcaaaat atctgtaagg aattgggagc acaattaaca aaggattatc aaggtaaacc
      541 attagtatgc gtgggtatct taaaaggctc agcaatgttt atgtcagatt taattaaacg
      601 aattgatacc catttatcaa ttgatttcat ggatgtttct agttatcacg gaggcactga
      661 gtcaactggt gaagttcaaa tcattaaaga tttaggttct tctattgaaa ataaagacgt
      721 attaattatt gaagatatct tagagactgg tactacactt aagtcaatta ctgaattatt
      781 acaatctaga aaagttaatt cattagaaat agttacttta ttagataaac caaaccgtcg
      841 taaagcggac attgaagcta agtatgtagg taaaaaaata ccagatgaat ttgttgttgg
      901 ttacggttta gattatcgtg aattataccg aaacttacca tatatcggta cgttaaaacc
      961 tgaagtgtat tcaaattaat tttttaatcg atttcagtta ttattactat gcgtttgaga
     1021 aataatagtg tagactcaaa aatatgaaaa atgtatttca tatatattta attttagaca
     1081 agacatatgt cttaaaaagt tgaaaaatat agagattgat aaaactaaca cagggtgtga
     1141 atgacattga tgttaagctc aattactagc ttataaaaca tgtcatatgt tacaattttt
     1201 gttagtttta ttatgggaag taggaggaaa tgacgcatgc agaaagcttt tcgcaatgtg
     1261 ctagttatcg taataatagg cgttattatt tttggtctat tttcatattt aaacggtaat
     1321 ggaaatatgc cgaaacagct tacatataat caatttactg agaagttgga aaaaggtgac
     1381 cttaaaactt tagaaatcca accacaacaa aatgtctata tggtaagtgg taaaacgaaa
     1441 aatgatgaag actattcatc aactatttta tataacaacg aaaaagaatt acaaaaaatt
     1501 actgatgctg ctaaaaagca aaacggtgta aaattaacga ttaaagaaga agaaaaacaa
     1561 agtgtctttg tgagtatact ttcaacatta attccagttg tagtcatagc gttattattt
     1621 attttcttcc taagccaagc acaaggtggc ggtagtggcg gtcgtatgat gaactttggt
     1681 aaatctaaag caaaaatgta cgataataat aaacgtcgtg ttcgtttctc tgatgtagca
     1741 ggggcagatg aagaaaaaca agaattaatt gaaattgttg atttcttgaa agacaataaa
     1801 aaattcaaag aaatgggatc taggattcct aaaggtgtct tacttgttgg acctccaggt
     1861 actggtaaaa cattacttgc tagagcggtt gcaggtgaag caggcgcacc attcttctct
     1921 attagtggtt cagactttgt agagatgttt gttggtgttg gtgcgagccg tgttcgtgac
     1981 ttattcgata atgctaagaa aaacgcgcct tgtatcatct ttatcgatga gattgatgct
     2041 gttggtcgtc aacgtggtgc aggtgttggt ggcggtcatg atgaacgtga acaaacccta
     2101 aaccaattat tagttgaaat ggatggattc ggtgaaaatg aaggtatcat tatgatagct
     2161 gctacaaacc gtcctgatat ccttgaccca gccttattac gtccaggtcg ttttgataga
     2221 caaattcaag ttggtcgtcc agatgtgaaa ggccgtgaag caattcttca tgttcatgct
     2281 aaaaataaac cacttgatga aacggttgat ttaaaagcga tttcacaacg tacacctggt
     2341 ttctcaggtg ctgatttaga aaacttatta aatgaagcat ctttaattgc tgtacgtgaa
     2401 ggtaaaaaga aaattgacat gagagatatc gaagaggcaa cggatagagt tatagccgga
     2461 cctgctaaga aatctcgagt tatttctaag aaagaacgta atattgttgc tcatcacgaa
     2521 gctggtcata caattatcgg tatggtactt gatgaggcag aagtagtgca taaagttact
     2581 atcgttccac gtggacaagc tggtggttat gcaatgatgc tacctaaaca agatcgtttc
     2641 ttaatgactg aacaagagtt attagataaa atctgtggtt tacttggtgg acgtgtatca
     2701 gaagatatta actttaacga agtatcaaca ggtgcttcaa atgacttcga acgtgcaaca
     2761 caaatcgcac gctcaatggt tacgcaatat ggtatgagta aaaaattagg gccattacag
     2821 ttcggtcata gcaatggtca agtattctta ggtaaagata tgcaaggtga gcctaattat
     2881 tcaagccaaa tcgcatatga aattgataaa gaagttcaac gaatcgttaa agaacaatac
     2941 gaacgttgta aacaaatttt attagagcac aaagaacaat taattttaat tgctgaaaca
     3001 ttattaacag aagaaacatt agttgctgaa caaattcaat cattattcta cgaaggtaaa
     3061 ttacctgaaa ttgattatga tgcagctaaa gttgttaaag atgaagattc tgaatttaat
     3121 gatggtaaat tcggtaaatc ttatgaagag attcgtaaag agcaattaga agatggacaa
     3181 cgtgacgaaa gtgaagatcg taaagaagaa aaagatattg ctgaggataa aaaagaagct
     3241 gataaatctg atgaaaaaga tgaaccagca catcgacaag ccccaaatat cgaaaaacct
     3301 tacgatccaa atcacccaga caataaataa tcgattatat tcagtacctc tttctatgat
     3361 aaagttatag aaagaggtac ttttatgatt taaggaatac gagcctgaga cataaaacaa
     3421 tgttcaatgc tctacaaagt tatattggca gtagttgact gaacgaaaaa gcgctagtaa
     3481 caagcttttt tcaattctag tcaggcgccc caacacagag aatttcgaaa agaaattcta
     3541 caggcaatgc gagttggggt gggacgacga aataaatttt ttgaaaatat catttctgtc
     3601 ccactcccat tatggcacga ataattaata atcaaacaag ttgcttcatt atttagaatg
     3661 atgggtgtag aataagtaca attgttgcat tttatgaagt aaagtaattt ttaaaatata
     3721 gagtaataga ggagattgaa ataatgacac acgattatat tgttaaagca ttagcatttg
     3781 atggggagat tagggcttat gctgctttga caactgaaac tgttcaagaa gcacaaacga
     3841 gacattatac atggccgaca gcatctgctg caatgggaag aacaatgaca gcaacagcta
     3901 tgatgggcgc aatgttgaaa ggtgatcaaa aattaacagt cactgtagat ggccaaggac
     3961 ctattggacg aattattgcc gatgcaaatg ctaaaggcga ggtgcgtgct tatgtagacc
     4021 atccacaaac tcattttcca ttaaatgagc aaggtaaact tgatgtaaga cgagcagtag
     4081 ggacaaatgg atctattatg gttgttaaag acgttggaat gaaagactat ttctctggag
     4141 caagtccaat tgtttcagga gaacttggtg aagattttac ttattattat gctacaagtg
     4201 aacaaacacc ttcatcggta ggtcttggtg tattggtaaa tcctgataat acgattaaag
     4261 cagcaggagg atttatcatt caagttatgc caggtgctaa agatgaaaca atttcaaaat
     4321 tagaaaaagc aattagtgaa atgacaccag tttctaaatt aattgaacaa ggattaacgc
     4381 cagaaggatt actaaacgaa atcttaggtg aagaccatgt gcaaatttta gagaaaatgc
     4441 ctgttcaatt tgaatgtaat tgtagtcatg agaaattttt aaatgctatt aaaggattgg
     4501 gcgaggctga gattcaaaat atgatcaaag aagatcatgg tgctgaagca
//
LOCUS       DATTQF010000169         4508 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.171, whole genome shotgun sequence.
ACCESSION   DATTQF010000169 DATTQF010000000
VERSION     DATTQF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.171"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            123..1613
                     /locus_tag="VB241_002442"
     CDS             123..1613
                     /locus_tag="VB241_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEP1245689.1"
                     /translation="MDDKQHTTSSDDERAENATSNQDQQTNSSKRVHLKRWQFISILI
                     GTIIITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQNSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENLV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            2014..2523
                     /locus_tag="VB241_002443"
     CDS             2014..2523
                     /locus_tag="VB241_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP1245690.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDLVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            2535..3605
                     /locus_tag="VB241_002444"
     CDS             2535..3605
                     /locus_tag="VB241_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEP1245691.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEVLYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNKMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            3622..4236
                     /locus_tag="VB241_002445"
     CDS             3622..4236
                     /locus_tag="VB241_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP1245692.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSSIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
     gene            4353..4508
                     /locus_tag="VB241_002446"
                     /pseudo
     CDS             4353..4508
                     /locus_tag="VB241_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1729 a          575 c          795 g         1409 t
ORIGIN      
        1 taaaataaac taagttgtga ctaaaaatta atcaattata gtgaaatatg gtgcgctatc
       61 ttgcataaat tgatatgatt aactacacag aatttaaaag tacataataa ggaagtgata
      121 caatggatga taagcaacac acaacttcat ccgatgatga acgcgctgaa aatgcaacaa
      181 gcaatcaaga ccaacaaact aattcatcga aacgcgttca cttaaaacgt tggcaattca
      241 tatcaatttt aattggtaca atcataataa cagctgtcat cacagttgtt gcatatattt
      301 ttataaatca aaaaataagt ggtttaaaca aaactgatca agcaaactta aataaaattg
      361 aaaatgtgta taaaatctta aatagtgatt attataaaaa acagaactct gacaagttga
      421 gtaaagctgc aattgatggc atggtcaaag aattaaaaga tccttattct gaatatttaa
      481 caaaagaaca aactaaatcc tttaatgaag gtgtttcagg tgattttgta ggtattggtg
      541 cagaaatgca aaagaaaaat gatcaaatta tggttactag ccctatgaag ggttctccag
      601 cagaacgtgc tggcattcgt cctaaagatg tcattactaa agtaaatgga aaatcaatta
      661 aaggaaaagc attagacgaa gttgtcaaag acgttcgcgg taaagaaaac actgaagtaa
      721 ctttaactgt tcaaagaggc agtgaagaaa aagacgttaa gattaaacgt gaaaaaattc
      781 atgttaaaag cgttgagtat aagaaaaaag gtaaggttgg agttattact attaataaat
      841 tccagaatga tacatcaggt gaattgaaag atgcagttct taaagctcac aaagatggtt
      901 tgaaaaagat tgttttagat ttaagaaata atccaggtgg actactagat gaagctgtta
      961 aaatggcaaa tatttttatc gataaaggaa aaactgttgt taaactagaa aaaggtaaag
     1021 atactgaagc aattcaaact tctaatgatg cgttaaaaga agcgaaagac atggatatat
     1081 ccatcttagt gaatgaaggt tcagctagtg cttctgaagt gtttactggt gcgctaaagg
     1141 actataataa agctaaagtt tatgggtcaa aaacattcgg taaaggtgtc gtacaaacta
     1201 caagagagtt taaagatggt tcgttgttaa aatatactga aatgaaatgg ttaacgccag
     1261 atggtcatta tattcacggt aaaggcatta aaccagacgt tactattgac acacctaaat
     1321 atcaatcttt aaatgtcatt cctaatacga aaacatttaa agttggagac gatgataaaa
     1381 atattaaaac tattaaaatt ggtttatcag ctttaggtta taaagttgat aatgaatcaa
     1441 cgcaatttga tcaagcttta gaaaatctag ttaaagcttt ccaacaagcg aataaactcg
     1501 aggtaactgg tgaatttaat aaagaaacga ataataaatt tactgagtta ttagttgaaa
     1561 aagctaataa acatgatgat gttctcgata aattgattaa tattttaaaa taaagcgata
     1621 cacactacta aaattttatt attattatgt taatgacacg cttcctaaat ttgcaaagat
     1681 agcaatttag gaggcgtatt tatttttatt gacgtctaac tctaaaagat ataaattaga
     1741 catttacaaa tgatgtaaat aacacaattt ctatcatcgc tgttaacaat tcatgcttta
     1801 atatactatg agcatatact ttttaaatag tattattcac tagttttaac aatcaattaa
     1861 ttggtatatg atacttttat tggttatttt tatcccatag tgtgataatt actatttttc
     1921 attcataata aaggtttaaa gcatgctaat agtgtgtaag attaacatgt actgaaaaac
     1981 atgtttaaca taacgatata aggagtgacg tacatgatcc gtctaggtaa aatgtcagat
     2041 ttagatcaaa tcttaaatct agtagaagaa gcaaaagaat taatgaaaga acacgacaac
     2101 gagcaatggg acgatcagta ccctctttta gaacattttg aagaagatat tgctaaagat
     2161 tatttgtacg tattagagga aaatgacaaa atttatggct ttattgttgt cgaccaagac
     2221 caagcagaat ggtatgatga cattgactgg ccagtaaata gagaaggcgc ttttgttatt
     2281 catcgattaa ctggttcgaa agaatataaa ggagctgcta cagaattatt caattatgtt
     2341 attgatttag ttaaagcacg tggtgcagaa gttattttaa cggacacctt tgcgttaaac
     2401 aaacctgcac aaggtttatt tgccaaattt ggatttcata aggtcggtga acaattaatg
     2461 gaatatccgc catatgataa aggtgaacca ttttatgcgt attataaaaa tttaaaagaa
     2521 tagaggtaat attaatgacg aaaatcgcat ttaccggagg gggaacagtt ggacacgtat
     2581 cagtaaattt aagtttaatt ccaactgcat tatcacaagg ttatgaagtg ctttatattg
     2641 gttctaaaaa tggtattgaa agagaaatga ttgaatcaca actaccagaa attaagtatt
     2701 atcctatttc gagtggtaaa ttaagaagat atatttcttt agaaaatgcc aaagatgtat
     2761 ttaaagtatt aaaaggtatt cttgatgctc gtaaagtttt gaaaaaagaa aaacctgatc
     2821 tattattttc aaaaggtgga tttgtatctg tgccagttgt tattgcagcc aaatcattaa
     2881 atataccaac tattattcat gaatctgact taacaccagg attagcgaat aagatagcac
     2941 ttaaatttgc caagaaaata tatacaacat ttgaagaaac gctaaactac ctacctaaag
     3001 agaaagctga ttttattgga gcaacaattc gagaagattt aaaaaatggt aatgcacata
     3061 atggttatca attaacaggc tttaatgaaa ataaaaaagt gttactcgtt atgggtggaa
     3121 gcttaggaag taaaaaatta aatagcatta ttcgcgaaaa cttagatgca ttattacaac
     3181 aatatcaagt gatacattta actggtaaag gattaaaaga tgctcaagtt aaaaaatcag
     3241 gatatataca atatgaattt gttaaagagg atttaacaga tttattagca attacggaca
     3301 cagtaataag tagagcaggg tcaaatgcga tatatgagtt cttaacatta cgtataccaa
     3361 tgttattagt accattaggt ttagatcaat cccgaggcga ccaaattgac aatgcaaatc
     3421 attttgctga taaaggttat gctaaaacga ttgatgaaga acaattgaca gcacaaattt
     3481 tattacaaga gctaaataaa atggaacagg aaagaactcg aattatcaat aatatgaaat
     3541 cgtatgaaca aagttatacg aaagaagctt tatttgataa gatgattaaa gacgcattga
     3601 attaatgggg ggtaatgctt tatgagtcaa tggaaacgta tctctttgct catcgttttt
     3661 acattggttt ttggaattat cgcgtttttc cacgaatcaa gactagggaa atggattgat
     3721 aatgaagttt atgaatttat atattcatca gagagcttta ttacgacatc tatcatgctt
     3781 ggggctacta aagtaggtga agtctgggca atgttatgta tttcattact tcttgtggca
     3841 tatctcatgt taaagcgcca caaaattgaa gcattatttt ttgcattaac aatggcatta
     3901 tctggaattt tgaatccagc attaaaaaat atattcgata gagaaagacc tacattgctg
     3961 cgtttaattg atataacagg atttagtttt cctagcggtc atgctatggg atcaactgca
     4021 tattttggaa gtagtatcta tttattaaat cgattaaatc aaggtaattc aaaaggtatt
     4081 cttatagggt tatgtgcagc tatgatttta ttgatttcca tatcacgtgt atatctaggt
     4141 gtacattatc caacagatat tattgccggc attattggtg gcgtattttg cattatttta
     4201 tcaacgttat tacttagaaa taaattaatc aattaaatag taaaaaaaca aaagcagtaa
     4261 acctaaagtg tcgtaagggt ttactgcttt tataaaacgt tgttataacg tatattgtct
     4321 tttacgggca tataaaaggg gaatatttga aaatgaccaa tccaacaaga acgaaacgtt
     4381 gtggggggat gttctatgtg gtattgataa tcattttcaa ctactattat acattagtga
     4441 gaatcattgt caattagaaa ctaaaacttt ttttgaatat tttttaagaa tagtaaataa
     4501 aacgcatg
//
LOCUS       DATTQF010000170         4504 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.172, whole genome shotgun sequence.
ACCESSION   DATTQF010000170 DATTQF010000000
VERSION     DATTQF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.172"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..460)
                     /locus_tag="VB241_002447"
     CDS             complement(<1..460)
                     /locus_tag="VB241_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HEP1245693.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIRPSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGELRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYNKL"
     gene            complement(1009..2670)
                     /locus_tag="VB241_002448"
     CDS             complement(1009..2670)
                     /locus_tag="VB241_002448"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP1245694.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVAAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(2667..3083)
                     /locus_tag="VB241_002449"
     CDS             complement(2667..3083)
                     /locus_tag="VB241_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP1245695.1"
                     /translation="MDKKVSIHTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(3176..3284)
                     /locus_tag="VB241_002450"
                     /pseudo
     CDS             complement(3176..3284)
                     /locus_tag="VB241_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3359..4369)
                     /gene="adhP"
                     /locus_tag="VB241_002451"
     CDS             complement(3359..4369)
                     /gene="adhP"
                     /locus_tag="VB241_002451"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP1245696.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         1354 a          939 c          605 g         1606 t
ORIGIN      
        1 ctaatttatt atatattttt cttgtatagc tatctggaat aaattcaata cgtccgaata
       61 tataaacaag taagacatct gctgtttcac ttccaatacc tttcaaagat aataattctt
      121 ttcttaattc acctttatat cgctcattaa tctcttgata attgaaatga tgtcgtgcta
      181 accatgttaa taatgtttta atcgtcagtg atttactttt ataaaagcca cttggacgta
      241 tcaatgattg taacgtttca ataggtagtt ctaatatatg atttggatta aaatgcgtgt
      301 gttctttaat ctgattcaat gcaatttctg catttcgcca tctagtattt tgaactaaaa
      361 ttgcacctaa catcatttca atgtcattat cagcaggcca ccaattttga ggtcccatat
      421 gcctatataa caatttatac aattcatcag ttcctaacat ttacatcctc cttttagagc
      481 cccttgagct ttagcgatta ggggctcttt tgcagttgct gcgcaagcaa accaactgga
      541 ttcctttaac ccccttgagc tttagcgatt aggggctctt ttgcagttgc tgcgcaagca
      601 aaccaactgg attcctttaa cccccttgag ctttagcgat taggggctct tttgcagttg
      661 ctgcgcaagc aaaccaactg gattccttta actccccttg agctttagcg attaggggct
      721 cttttgcagt tgctgcgcaa gcaaaccaac tgtattcctt taaccccctt gagctttagc
      781 gattaggggc tcttatgcag ttgtgcgcaa gcaagccaac tgtattcctt tccccccttg
      841 agctttagcg attaggggct cttttgcagt tgctgcgcaa accaactgta ttcctttaac
      901 cttattcaca tactaagcat tccccaatat gtcacatacc aaataaaaaa acactccaac
      961 acaaagtatc tttcgagaaa ctctacgttg gagtgtatat aaatgttctt acattgattc
     1021 aggtgcgctt acaccgacca ttgccaatgc atttttcaat gtaattctga ccgcttcaat
     1081 catagcaaca tgtgcttttg ttttttcaat atcatctgtt aacacttttt cagcattata
     1141 gaatttatgg aaatgagaag ctaaatcttg aatataatta gtaattctat gtgctgatct
     1201 atgctcagca gcactttcaa ttgtaggttc gaaatcagct acttttttca acaattcgat
     1261 cgctttttca tttgtaatcg ttgtaaaatc attcgcagca gctacctcaa ttccttgctc
     1321 tttcgcttgt tttaaaattg aacaaatacg cgcatgtgca tattgagcat agtaaactgg
     1381 attgtcttga gattgctctt tcgctaattc catatcaaaa tcaaagtgac tatcaggact
     1441 acgcatagtt aagaaataac gtgcagcgtc aacgccaact tcatccataa tttctcttaa
     1501 tgtaatcgca ttaccagtac gtttactcat cttcacttct ttaccatttt ccattaaacg
     1561 aaccatttgc atgatttgaa tttctaaacg attactatct acaccaaacg tttcaagaga
     1621 tgctttcaaa cggttaatat aaccatgatg atcagcacca aataaatcga ttaaaatgtc
     1681 attaccacgt ttaactttat cgaagtggta cgcaatatct ggtaagaaat acgtatatgt
     1741 accgtcattt ttaattaata ctctgtcttt gtcgtcttta aaatcagttg tacgtaacca
     1801 tgtagcgcca tcagcttcat acgtataacc taattctttc attttagcta aaacttcaag
     1861 aatttcgcct ttttcatata aagatgtttc actaaaccaa ttatcaaaat gcgtattgaa
     1921 ctctgctaaa tcatttttca gtttagccat ttcgtattct acgcctaatt ttctaaattc
     1981 tttcaaacgt gcttcttcag aataatcttt aatttcaggg tgtttctctg ctaaatcttt
     2041 acctatttca ataatatctt tgccattata gccatcttct ggcattgaat aactattgtc
     2101 acctaaagct tcaaagaaac gtgtttcaat cgaacgcgct aagttagtaa tttgattacc
     2161 agcatcatta atataatatt cacgtgttac attatagcca gctgcagtta aaatattagc
     2221 taaagcatca ccaactgctg catttctagc atgaccaata tgtaaatctc ctgtagggtt
     2281 agctgaaaca tactcaagca atacattttg accttttgat tcatttacat gtccaaattg
     2341 atcacctttt tcaattgctt caggaataat tgctgttaaa tactgattat ctaagtaaaa
     2401 attaatgaat cctggaccag caatgtcaat ttgttttaca tgtgcttttt cagtatctaa
     2461 gttatcaaca atcgcttgag caatttcacg aggattacgc tttgcaatct tagtcagtac
     2521 catcgcaata ttagtagcat aatctccatt ttttgtatct ttaggaactt caattttaat
     2581 atcaggaatc tcatctgcta atcctgcttt gttaatactt gctgcaattt cttctactaa
     2641 tgtttgtttc acttgatcaa taatattcat tcgcctatct ccttatagtt aatttcatat
     2701 tgataagaac ccattttttc attatcttga tataattcat aatgaatctt taacttgcca
     2761 ccgtctccac ttacgaaatg taaaatgcgt aatgttttaa cttctagtgg aattcgtcca
     2821 gccgatatat cgtaaaaagt tgttgtcgtt tgtccttcaa caaaatgcaa attcatatta
     2881 atgtcgcctt tacgaatcaa cttaacgcta tcatcatcca cttttattgt aacattaact
     2941 gttgcatcct caatttgttc tacataccga ataaagttag attgcctttg ttgccaagtt
     3001 ccttcagtag taaattcaaa tttttctttt tcgttgtgct gtttcaacac ttgctttgta
     3061 tgaatactta cttttttatc caatttacta ctcaccttaa tcttcgcata atagaaatat
     3121 tatagcataa aataaggtca tcttgttatg ccttcttaaa gctaaatagg ttcaattaat
     3181 taacaattcc atacttaatc aacgcttgat atatgtaagc cattttataa aatacaaaca
     3241 aaacctcaaa accaaaaaaa cacgagattt tcactatctt tcatgaaaac ctcgtgtttg
     3301 attataatga tagtgttcag agcactacct tctttatcta taaagtcatt tgatattttt
     3361 aaaatttaat aaccattcta ccagttatag taccattttc catttcttca aaaatatcat
     3421 tgatttcttc taattttctt aattgaactt taggtgttac tttattttca gcagcaaatt
     3481 caaacgcttc acgtaagtct tgtcttgtac caacaagtga acctactact tcaataccat
     3541 ctagcactaa tcttgggata tctaagttca ttttatcaac aggtaaacca acggcaacaa
     3601 ctcttgcacc agcttttaca acatcaaccg cttggttaaa tggcgtctta gcaactgaag
     3661 ttaccactgt tgcatctaat cctttgttat cagttaattt cataacttct gcaactggat
     3721 caacatcttt agaattaata atagcatctg cacctaattc ttttgcgaat gctaatttat
     3781 catcattaat gtcgaaggca acaattttag cccccataac gtttttagca tattgtaaag
     3841 ctaggttacc taaaccacct ataccaaaaa cacctaacca ttgtccaggt ttaacattac
     3901 ttacttttac agctttataa gttgtcacac ctgcgcatgt aatagaagac gctgctgctg
     3961 gatctaattt ttcaggtact ttcacagcat agtctgcagt aacaataact tgttcagcca
     4021 ttgcaccatc tactgtataa ccagcatttt tcacactacg gcaaagtgtt tcacgacctg
     4081 ttgtacaata ttcacatctt ccacagcttt cgaacatcca agcgatagac acacggtctc
     4141 caatttttaa tgattctaca tcttctgcaa cttcgatgac tttaccaata ccttcatgac
     4201 ctaaagtaac gcctgtaaca tcaccaaaat cagcattctt aacatgtaaa tcggtatgac
     4261 aaacgccaca atattccgtt tgtaccaatg cttcaccagg ttttaaagct cttaactttt
     4321 tgtcctcaat acttactttg tgatctttcg ttacaactgc tgctctcata ataatgtcct
     4381 ccttatagtc actccagtat gtatgatatt aattggtgat tactttcaaa aatgaaaacg
     4441 aataattata gatgtaattt ttatttgtaa tagacgccaa attaaaacat actattgtga
     4501 cttt
//
LOCUS       DATTQF010000171         4478 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.173, whole genome shotgun sequence.
ACCESSION   DATTQF010000171 DATTQF010000000
VERSION     DATTQF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.173"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            81..1040
                     /gene="hyl"
                     /locus_tag="VB241_002452"
                     /pseudo
     CDS             81..1040
                     /gene="hyl"
                     /locus_tag="VB241_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
     gene            1494..1727
                     /locus_tag="VB241_002453"
     CDS             1494..1727
                     /locus_tag="VB241_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245697.1"
                     /translation="MVFKDWEINVVYSGKHEVVTNENSLFVIDEDYDVAIAINYLDNK
                     LKVSHVNYGSEFTIDASNKVLALMIHNPNIDEN"
     gene            2337..2522
                     /locus_tag="VB241_002454"
     CDS             2337..2522
                     /locus_tag="VB241_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245698.1"
                     /translation="MNKHINKKTTKHLEDYKKVQQRNSEETNRGCLKSAAMLIVLFLF
                     IIFGIVACSTHVNFFFQ"
     gene            complement(2771..3121)
                     /gene="scc"
                     /locus_tag="VB241_002455"
     CDS             complement(2771..3121)
                     /gene="scc"
                     /locus_tag="VB241_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-C"
                     /protein_id="HEP1245699.1"
                     /translation="MKFKKYIVAGTLAVLLSTTAVSTLDGNKADASSKKDYIIQSEFH
                     DKRIAEELKSLLDQSYVNDLAAGSLNPYYKRMIMMNQYRAKAALKSNNFAKMAEAKVG
                     LENIYKEIDEIINR"
     gene            complement(3273..3770)
                     /gene="efb"
                     /locus_tag="VB241_002456"
     CDS             complement(3273..3770)
                     /gene="efb"
                     /locus_tag="VB241_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HEP1245700.1"
                     /translation="MKNKLIAKSLLAIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNILEYNDGTFKYQSRPKFNTTPKYIKFRHDYNIVEYNDGTFEYGARPQFNKPAAKTE
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYNVKKMILQERIDQVLK
                     QGLVR"
     gene            complement(4027..>4478)
                     /locus_tag="VB241_002457"
     CDS             complement(4027..>4478)
                     /locus_tag="VB241_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245701.1"
                     /translation="TNKKKPFLFLKTLFMGVVFIFIGYISLAISAVIIYGIIQFITID
                     FGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAVN
                     EKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT         1615 a          659 c          614 g         1590 t
ORIGIN      
        1 tgtctcaact gcattattct aaattgacat attgattatt gtttcctcaa atcgttttaa
       61 aaaatagaag gatgatgaaa atgaaaacac gtatagtcag ctcagtaaca acaacactat
      121 tgctaggttc tatattaatg aatcctgtcg ctaatgccgc agattctgat attaatatta
      181 aaaccggtac tacagatata ggaagcaata ctacagtaaa aacaggtgat ttagtcactt
      241 atgataaaga aaatggcatg cacaaaaaag tattttatag ttttatcgat gataaaaatc
      301 acaataaaaa aatattagtc atcagaacga aaggtaccat tgctggtcag tatagagttt
      361 atagcgaaga aggcgctaat aaaagcggtt tagcctggcc ttcagcattt aaggtatagt
      421 tgcaactacc tgataatgaa gtagcacaaa tttctgatta ctaccctaga aattcaattg
      481 atactaaaga atatatgagt acattaactt atggattcaa cggtaacgtg actggtgatg
      541 attctggaaa aattggcggt ttgattggtg caaatgtttc aattggtcat acactcaaat
      601 atgttcaacc agatttcaaa acaattttag aaagtccaac tgataaaaaa gtaggttgga
      661 aagttatatt taacaatatg gtgaatcaaa attggggacc atatgacaga gattcttgga
      721 acccagtata tggcaatcaa cttttcatga aaacaagaaa cggttcaatg aaagcagcag
      781 agaacttcct tgaccctaac aaagcaagtt ctttattatc ttcaggattc tcaccagact
      841 ttgctacagt tattactatg gataggaaag caaccaaaca acaaacaaat atagatgtaa
      901 tatacgaacg agttcgtgat gactatcaac tacattggac ttcaacaaat tggaaaggta
      961 ccaatactaa agataaatgg acagatcgtt cttcagaaag atataaaatc gattgggaaa
     1021 aagaagaaat gacaaattaa tggttatgta cactcaaata gtcacataat aatattagcc
     1081 gataacatca gaaatgatgt gcgtcggcta ttttagttgc agtataaaat taaacatcat
     1141 gaatagtaat atacctatta tgcttttcag tatatattca cggctaaaat catgattacg
     1201 gtactgattt cttaactaca aacatttgaa attcattgtt gtcaaaccct ttaattaaaa
     1261 gttttgaaat tgttcaaaac ttattgaaca cattatgtgt taatatattt gcagaattat
     1321 tttaaaatcg ttttaaaact atactgaaat aagaagataa aaagtataat aagtacatat
     1381 aattgaaaat aatttctaga aatctattta tctctaatct agatattgta agaagttgta
     1441 tgaataagtt aaacttagat tgaatcagtt taaatgttca aggaggatga caaatggttt
     1501 ttaaagattg ggaaattaat gttgtttact cagggaaaca tgaagtagtt acaaacgaaa
     1561 atagtttatt tgttattgat gaagattatg atgtcgcaat cgcaatcaat tatttagata
     1621 ataaattaaa agttagtcat gttaactatg gctctgaatt tacaattgac gcatctaaca
     1681 aagtacttgc gttgatgatt cataatccaa atattgacga gaattaaata ttaaaaaaca
     1741 tttagaaaca taaaggcata ctatatatag tcttttaaac gtataacgaa cgaaaattag
     1801 aaattcggat attcattaca ctattaaaat gcaatttttc aaagcattta aaaacaacag
     1861 ttaaggtggt gaccaaaaat taacattgtg tataacatca ttgtatatat agcatgcatg
     1921 attaacttcg gactgattat cgtcccattg ctaactacca atgacgcaga ttttgtttgg
     1981 acttcttcgt cattaatata tatgatcctt ttaacactac ttatattaat tgtttatatt
     2041 aaaactaaag atagtttaca gctaacaata tttatactta gtgtggcatt aatccttcta
     2101 tactgtgcac ctttactgtt catttatgta ttctaatgtt tcattaatat tttaatacgt
     2161 cagtttaaaa acaaacaaat tggaaaataa acgaaacaag ttatcatcta atttaacatg
     2221 atactcactt aaaatttgaa acaacgagca gcctaactta agctttatat ctttatttca
     2281 agttatgttt tcaactattt taatgcaatt tacatttaga ttaaggagta aacatcatga
     2341 ataaacatat aaataaaaag accacaaagc atcttgaaga ttataaaaag gttcaacaaa
     2401 gaaattcgga ggaaacaaat agaggttgtt taaaaagcgc cgccatgctt attgtcttat
     2461 ttctattcat tatatttggc atcgtggcat gttcgacaca cgttaacttt ttcttccaat
     2521 aatatactca ttgagtaaaa atcagaacgt ttcatactac gtttaaattt atctatggga
     2581 gcactataaa tattttttag attgtacaaa gcaattttgt gcataatatt tctaactatt
     2641 atatttatat ttgtctcccc tattttttgt atacattgct catacataaa ttttaaattg
     2701 cagaattttt aactgtacaa ataaaaaaac aggcgaagac aattctattt ttgtctctgc
     2761 ctgtttttta ttatctattt ataatttcat caatttcttt gtaaatgttt tctaatccaa
     2821 ctttagcttc agccattttt gcgaaattat tactttttag tgctgctttt gctctatatt
     2881 ggttcatcat aatcatacgt ttgtagtatg ggtttaagct tcctgcagct aaatcattta
     2941 cataagattg atcaagtaat gatttcaatt cttcagcaat tcgtttatca tgaaactcac
     3001 tttgaattat atagtctttc ttactacttg catctgcttt attcccatct aacgttgata
     3061 ctgctgttgt tgataatagt actgctaatg ttcctgctac tatatatttt ttaaatttca
     3121 ttgtaaattc tccttttaaa attgatactt aatgattata tagtcaattt taggaaagtt
     3181 acattaacct ggcattaaat aacatagtcg ttttattaat aaaaaataat ataatattaa
     3241 attattttga acttataata atgacagaag tattatctca ctaatccttg ctttaatact
     3301 tgatcaattc gctcttgcaa tatcattttc ttaacattgt attcaaatga tactaagttg
     3361 actgcctttt gtgcttttct atgtgcgctc acagtatgtg ttttttcaaa ttctctcaca
     3421 agattttggg cttgaatcaa tttttgttct tttttaatag ttgcctcagt tttcgctgct
     3481 ggtttattaa attgtggacg tgcaccatat tcgaatgtac cgtcattata ttccacgatg
     3541 ttataatcat gtctaaactt aatatattta ggtgttgtat taaattttgg tctagattga
     3601 tatttaaaag taccatcatt gtactctagg atattgtgat taatactcac tggtttcttt
     3661 tctcttggac cgtatccttc gcttgcatct gcagttgacg caattgtagt tgtagtaata
     3721 ccaattgccg ctatcgctag taatgatttt gctatcaatt tatttttcat gttaattatc
     3781 ctccaaatta ttatcttaca tttattaagt tatcctattt atacaacttt tgaaattaac
     3841 tagttattaa ttaactataa acactttata atatagacaa aatatagtta tctatcttgt
     3901 gtctcatcaa tacttataca ctagtcaaaa atttcatgcc tctacttttc gaatttcaaa
     3961 aataaaacta cacaacaaaa agacaccatt tgaatgttag tgtctgatgg tataatttca
     4021 tagattttat ttgtaacttc tcattctata gttaaatatt gtaacccagc taataatcaa
     4081 agttggtaga attactaaat tcaaattaga cacgtcaaaa aagactgatt ggaaattgtc
     4141 gatattaata tcattaatgt ccattttttc atttactgct tgatagatcg caaaataaac
     4201 taagaaccat tgaattaaat gttctaaaac gacaacctct gttaaatcga cattcttttt
     4261 tctaaaaagt aatctaacta taaataattg gaatatactt gaaatcaaga tcaaaataat
     4321 acccattaag aaaaaactac caaaatcaat tgtgataaat tgaataatac cataaataat
     4381 tacggcagat attgccagtg aaatatatcc tataaagata aacaccacac ccataaagag
     4441 tgtctttaag aacagaaaag gcttcttttt atttgtaa
//
LOCUS       DATTQF010000172         4427 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.174, whole genome shotgun sequence.
ACCESSION   DATTQF010000172 DATTQF010000000
VERSION     DATTQF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.174"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            66..2588
                     /gene="mprF"
                     /locus_tag="VB241_002458"
     CDS             66..2588
                     /gene="mprF"
                     /locus_tag="VB241_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEP1245702.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILN
                     KITWVRWVLYAVSLFLPLFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDVHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYLLLAIHTSACLLLLLNVVGIYK
                     QSRRAIIYAMISIILIIVATLFTYASYILITWLVIIFALLIVAFRRARRLKRPIRMRN
                     LVAMLLFSIFILYINHIFIAGTFYALDVYTIEMHTSVLKYYFWITILIIAIIVGAIAW
                     LFDYQFSKVRISSNIEECEEIIDQYGGNYLSHLIYSGDKQFFTNEDKNAFLMYRYKAS
                     SLVVLGDPIGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNETYLSKAPIGVMRNENNEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2791..3300)
                     /gene="msrA"
                     /locus_tag="VB241_002459"
     CDS             complement(2791..3300)
                     /gene="msrA"
                     /locus_tag="VB241_002459"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP1245703.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3437..4420
                     /locus_tag="VB241_002460"
     CDS             3437..4420
                     /locus_tag="VB241_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP1245704.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
BASE COUNT         1423 a          605 c          787 g         1612 t
ORIGIN      
        1 tatttttatg gtttatatga tatattttga tttataacag aaataattag aattgatgtg
       61 aaaaaatgaa tcaggaagtt aaaaacaaaa tattttcaat cttaaaaatt acgtttgcta
      121 cagctttatt tatttttgta gtaatcacgt tgtatcgaga gttatctggg attaacttta
      181 aagatacttt ggttgaattt agtaaaatca accgtatgtc ccttgtctta ttatttattg
      241 gtggtggggc atcgcttgtt attctctcaa tgtatgatgt gattttatct agagctttaa
      301 aaatggatat ttcattagga aaagttttaa gagtaagtta tatcattaat gcattaaatg
      361 cgattgtagg ctttggtggc tttattggtg ccggtgtaag agcaatggtt tacaaaaact
      421 atacgcatga taaaaagaaa ttggttcact ttatttcatt aatactgatt tcaatgttga
      481 caggtttaag tttgttatca ttgttaatcg tattccatgt ttttgatgca tcattaattt
      541 taaataaaat tacgtgggtg agatgggtac tttatgctgt gtcgttgttc ttaccattat
      601 ttattatata ttcaatggtt agaccgcccg ataaaaacaa tcgttatgta ggcttgtatt
      661 gtacattagt gtcatgtgtg gaatggttag ccgctgcagt tgtattatac ttctgtggtg
      721 tcatagttga cgtccatgtc tcatttatgt catttattgc aatattcatt attgctgcat
      781 tatctggatt agtcagcttt attccaggtg gttttggtgc cttcgactta gttgttttat
      841 taggctttaa aacgttaggt gtacctgaag aaaaagtatt attaatgcta ctactttatc
      901 gctttgcgta ctatttcgta ccagtgatta ttgcattaat cctttcatca tttgagtttg
      961 gtacttcagc taagaaatat attgaaggat ctaaatattt cattccggct aaagatgtga
     1021 catcgttttt aatgtcgtat caaaaagata ttattgctaa aataccatca ttatcattgg
     1081 caattttagt attctttaca agtatgattt tctttgtaaa taatttgacg attgtatacg
     1141 atgctttata tgatggcaat catttaacgt attatctttt attggcaata catacaagtg
     1201 cctgtttatt acttttactt aatgttgtag gtatttataa acaaagtaga cgtgccatta
     1261 tttatgcaat gatttcgatt atcttaatta ttgtcgcgac gttattcact tatgcgtcat
     1321 acattttaat aacttggtta gttattatct ttgctttact tattgttgct ttccgtagag
     1381 cacgtaggtt aaaacgtcca attcgaatga gaaatctagt cgcaatgctt ttgttcagca
     1441 tatttatact ctacatcaac catatattta tagctggaac attttatgct ttagatgttt
     1501 atacaatcga aatgcatacg tctgtactta aatattattt ctggattacc attttaatta
     1561 tcgcaattat tgttggtgca attgcgtggt tattcgatta tcaatttagt aaagtgcgta
     1621 tatcttctaa tattgaagag tgcgaagaga ttattgatca atatggtggg aattatttaa
     1681 gtcatttaat ttatagtggt gataaacagt tctttactaa tgaagataaa aatgcatttt
     1741 taatgtatcg ctataaagca agttcgttag tagttcttgg cgatccaatc ggtgatgaaa
     1801 atgcctttga tgaattgtta gaagcattct ataattacgc tgagtattta ggctatgatg
     1861 ttatattcta tcaagttaca gatcaacaca tgcctttata tcataatttc ggcaaccaat
     1921 ttttcaaatt aggtgaagaa gcaattattg atttaacgca attttcaact tcaggtaaaa
     1981 aacgccgtgg atttagagcg actttaaaca aattcgatga acttaatatt tcattcgaaa
     2041 ttattgaacc accgttttca actgaattta taaatgaact tcaacatgtc agtgatttgt
     2101 ggttagataa tcgtcaggaa atgcatttct ctgttggtca atttaatgaa acatatttat
     2161 ctaaagcgcc aattggtgtg atgcgcaatg aaaataatga agtaattgca ttttgtagtt
     2221 taatgccaac atactttaat gatgccattt cagtcgattt aattagatgg ttgccagagt
     2281 tagatttacc gttaatggat ggattatact tgcacatgtt actttggagt aaagaacaag
     2341 gttatacaaa atttaatatg ggtatggcaa cgttatcgaa cgttggtcaa ttgcattatt
     2401 catatttaag agaaagactt gcaggtcgtg tctttgaaca tttcaacggt ctatatcgtt
     2461 tccaaggatt acgtcgttat aaatctaaat ataatccgaa ttgggaacca cgctttttag
     2521 tttatcgtaa agataattcg ctttgggaat cactttctaa agtaatgcgt gtaatacgtc
     2581 acaaataatt aaaatccaag tgctaatagg tatacagtta tgcctgaagc gcttggattt
     2641 tttggtttct ataaagaatg atttataaaa aacggcacag ataagaatat ttaagtttcc
     2701 tatctgtacc gtattttaat attgaaatga acttaagtta agtcgcctaa cttttattat
     2761 cagtccaatc agtttcatca agtgtaaatg ttattgttta tttttgtatt cttggcgtat
     2821 tttttgttct tctgcataac gctctggatt tttcttataa aaatcttggt gatagtcttc
     2881 ggctttgtaa aattgtgacg ctggtaatat ttttgttgca attgccttat cagcattaat
     2941 cgtattttta agctgctcga tataagtctc agcgagttct ttttgatgat cattagtgta
     3001 gaaaatagct gtttgatatt gaggaccacg gtcttgatat tgaccacctg tatctaatgg
     3061 gtcaatgact gagaaaaata tttctaataa cttattgtat gagaataatg caacatcata
     3121 ttgaatttca acagtttcta aatgtccact cgtacctgat tttacttgtt cgtaagtagg
     3181 gttttcaata tgtccgccca tatatccaga agttactttt tctatgccgt caaaggtgtc
     3241 aaatggtttc gtcatacacc aaaagcaacc tccggcaaaa taagctgtat taatattcat
     3301 ttttgacatc ctttcattag accttagtac gatttattaa gaaatcactt gctttttgaa
     3361 ttgtttttat ataacgttaa tatgtgatta ttatagtaca caaaaatcta tttttgaaga
     3421 tataaggtag gtaaatatgg ataaagaaac taatgacaac gaatatagac gtcaaagtga
     3481 acatcgcact tcggcgccta agcgaaaaaa gaagaagaaa attaggaaat tacctatcat
     3541 tcttctgatt gttgtaattt tacttatcgc attagttgta tatattgtac atagttacaa
     3601 tagcggtgta gaatatgcca agaaacatgc gaaagatgtt aaagtacatc aatttaatgg
     3661 accagtaaaa aatgatggta aaatttctat tcttgtactc ggtgcagata aagcacaagg
     3721 tggacaatca agaacagatt ctatcatggt tgttcaatat gactttatca ataaaaagat
     3781 gaaaatgatg tctgtcatgc gtgatattta tgcagatatt ccagggtatg gaaaacacaa
     3841 aattaattca gcatacgctt taggtggtcc agagctactt agaaaaacac ttgataaaaa
     3901 tttaggaatt aatcctgaat attatgcagt agttgatttt actggatttg agaaaatgat
     3961 tgatgaatta atgccagaag gtgtaccaat taatgtcgaa aaagatatgt cgaaaaatat
     4021 tggtgtatct ttgaaaaagg gtaaccatag gttgaatggt aaagaattac ttggttatgc
     4081 aagattccgt cacgaccctg aaggtgactt cggacgcgtg cgacgtcagc aacaagtaat
     4141 gcaaactttg aaaaaagaaa tggttaattt tagaacagtt gttaaattac caaaagttgc
     4201 aggtatttta agaggctatg tgaatacaaa cattcctgat tcagggatct tccaaacagg
     4261 tttgagtttt ggtatccgag gtgaaaaaga tgttaagtca ttgactgtgc caatcaagaa
     4321 ctcatacgaa gatgtcaata caaatactga tggtagtgca ttacagatta ataaaaacac
     4381 aaataaacaa gctattaaag actttttaga tgaagattaa aaataaa
//
LOCUS       DATTQF010000173         4418 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.175, whole genome shotgun sequence.
ACCESSION   DATTQF010000173 DATTQF010000000
VERSION     DATTQF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.175"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            369..4295
                     /locus_tag="VB241_002461"
     CDS             369..4295
                     /locus_tag="VB241_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEP1245705.1"
                     /translation="MLNRENKTAMTRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     IGSQEAKAAEVTNKEMKEDATSVNNDQVSKKVDTGQLNNDGNTSKVDTEQLKDENINK
                     VANQKEVTRVENSVALEKNNNSQPSENGKLQTSDVKKTDDNNATSDDQVTTTVNSQQL
                     NTDNTTANATMSSAPINVSKDDLKINSEEVRNVEEKRDNTDNANSSELIKPKSTAPKR
                     LNTRMRMAAVQPSSTDSKNVNDLITSNTTLTVVDADKNNKIVPAQDYLALKSQIKVDD
                     KVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTANNLITYTFT
                     DYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNDTTKTTANIQYPDYVSRDN
                     NSIGSAFTETVSHAGNAEDPGYYKQTVYVNPSEKSLTNAKLKVEAYHKDYPDNVGQIN
                     KDVTKIKIYQAPKDYVLNKGYDVNTNQLIDVTEQFKDKITYGANDSVNVDFGSINNSY
                     VVMVDTKFEYTTSESPTLVQMATLTSDGNRSVSTGNALGFTNNQSGGAGQEVYKIGNY
                     VWEDTNKNGVQDLGEVGVKGVTVVAYDNKTNKEVGRTITDDKGGYLIPNLPNGDYRVE
                     FSNLPQGYEVTPSKQGNNEELDSNGVSSVITVNGKDNLSADLGIYKPKYNLGDYVWED
                     TNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGNYKVEFTTPE
                     GYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNYVWEDTNKDG
                     KQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEFETPSGYTPT
                     QVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTNKNGVQDKDE
                     KGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGYTPTSVTTGN
                     DTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSESDSDSDSESDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSATK
                     DHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1811 a          624 c          790 g         1193 t
ORIGIN      
        1 aaataataca atatgaccca ggtccttgtg gcctggtttt tctgtttaga aatttccaga
       61 tataaaacaa aaaatcttta aggaatagat ataatttaat ttgttacaga aatgtaattg
      121 tctatgaatt agaaaagcta agatattaat tttaagggca aataatacta tttcttatga
      181 taattaaata aaaaatacaa aactcataaa aacttttatt taactacaaa tgttaaataa
      241 cgttttacga taaagaaaaa taattaaagt attgtgattt gtctaaaaat gtaatactat
      301 attgatatac atttttgtat ttaaaacaat tgtattattt aaaaatttga tgaattagga
      361 gtaatctaat gctaaacaga gaaaataaaa cggcaatgac aagaaaaggc atggtgtcaa
      421 atcgattaaa taaattttcg attagaaagt acacagtggg aacagcatca attttagtag
      481 gtacaacatt aatttttggt atagggagcc aagaagctaa agctgcagaa gtaaccaaca
      541 aggaaatgaa ggaagatgca acatcagtaa ataatgatca agtgtctaaa aaagttgata
      601 cagggcaact aaataatgac ggtaatacta gtaaagttga tacagagcaa ctaaaagatg
      661 agaatattaa taaagttgct aatcaaaaag aagtaactag agtagaaaat agtgtcgctt
      721 tagaaaaaaa taataactct caaccatctg aaaatggaaa attacaaacg tcggatgtta
      781 aaaaaactga tgataataat gcaacttcag atgatcaagt tactactaca gttaattcac
      841 agcaattaaa tactgataac acaactgcaa atgcaacaat gtcgtcagca ccaattaatg
      901 taagtaaaga tgacttaaaa attaatagtg aagaagtaag aaatgtagaa gaaaaaagag
      961 ataatactga caatgcaaat agttcagaat taataaagcc gaaaagcaca gcgcctaaac
     1021 gtttgaatac gagaatgcgt atggcagcag tacaaccatc atcaacagat tctaaaaatg
     1081 ttaatgattt aatcacatca aatacaacat taactgtcgt tgatgcagat aaaaacaata
     1141 aaatcgtacc agcacaagat tatctagcat taaaatcaca aattaaagtt gatgacaaag
     1201 ttaaatcagg tgattatttc acaataaaat actcagatac agtacaagta tatggattga
     1261 atccagaaga tattaaaaat attggtgata taaaagatcc aaataatggg gaaacaattg
     1321 caactgcaaa acatgatact gcaaataatt taattacata tacatttaca gattatgtcg
     1381 atagatttaa ttctgtacaa atgggaatta attattcaat ttatatggat gctgatacaa
     1441 ttccagttag taaaaacgat gttgagttta atgtaacgat tgggaatgat acaacaaaga
     1501 ctactgcaaa cattcaatac ccagattatg tttctagaga taataattct ataggttcag
     1561 catttactga aactgtctca cacgcaggta atgcagaaga cccgggttac tataaacaga
     1621 ctgtttatgt aaatccatcg gaaaaatctt taacaaatgc taaattaaaa gttgaagctt
     1681 atcataagga ttatcctgac aatgtaggtc aaataaataa agatgtaact aaaattaaaa
     1741 tttatcaagc accaaaagac tatgttttaa acaaaggtta tgatgttaat acaaatcagc
     1801 taatagatgt aacagaacaa tttaaagata agattacata cggagctaat gacagtgtaa
     1861 atgttgattt tggtagcatt aataattctt atgtagttat ggtggataca aaatttgaat
     1921 atacaactag tgaaagtcca acacttgttc aaatggcaac tttgacttct gatggaaata
     1981 gatccgtttc tactggtaat gctttaggat tcactaataa ccaaagtggc ggcgctggtc
     2041 aagaagtata taaaattggt aactacgtat gggaagatac aaataaaaac ggtgttcaag
     2101 atttagggga agttggagta aaaggtgtaa cagtggttgc atatgacaat aaaactaata
     2161 aagaagttgg aagaacaatt actgatgaca aaggtggata cttgattcca aacttaccta
     2221 atggagatta ccgtgtagaa ttttcaaatt taccacaagg ttatgaagta accccttcaa
     2281 aacaaggtaa taatgaagaa ttagattcaa acggtgtatc atcagttatt acagttaatg
     2341 gcaaagataa tttatctgct gacttaggta tttacaaacc taaatacaat ctaggtgact
     2401 atgtatggga agatacaaat aaaaatggta tccaagacca agatgaaaaa ggtatttcag
     2461 gtgtaacagt tactttgaaa gatgaaaacg gtaacgtgtt aaaaacagta acaacagatg
     2521 ctgacggtaa gtataaattt actgatttag ataacggtaa ttataaagtt gaatttacta
     2581 caccagaagg ctatacaccg actacagtaa catccggtag cgatattgaa aaagactcta
     2641 atggtttaac aacaacaggt gttattaatg gtgctgataa catgacatta gatagtggat
     2701 tctacaaaac accaaaatat aatttaggta attatgtatg ggaagataca aataaagatg
     2761 gtaagcagga ttcaactgaa aaaggtattt caggcgtaac agttacattg aaaaatgaaa
     2821 acggtgaagt tttacaaaca actaaaacag ataaagatgg taaatatcaa tttactggat
     2881 tagaaaatgg aacttataaa gttgaatttg aaacaccatc aggttacaca ccaactcaag
     2941 taggttcagg aactgatgaa ggtatagatt caaatggtac atcaacaaca ggtgtcatta
     3001 aagataaaga taacgatact attgactctg gtttctacaa accgacttac aacttaggtg
     3061 actatgtatg ggaagataca aataaaaacg gtgttcaaga taaagatgaa aagggcattt
     3121 caggtgtaac agttacgtta aaagatgaaa acgacaaagt tttaaaaaca gtaacaacag
     3181 atgaaaatgg taaatatcaa ttcactgatt taaacaatgg aacttataaa gttgaattcg
     3241 agacaccatc aggttacaca ccaacttcag taactactgg aaatgatact gaaaaagatt
     3301 ctaatggttt aacaacaaca ggtgtcatta aagatgcaga taatatgaca ttagacagtg
     3361 gtttctataa aacaccaaaa tacagtttag gtgattatgt ttggtacgac agtaataaag
     3421 atggcaaaca agattcaact gaaaaaggta tcaaagatgt taaagttact ttattaaatg
     3481 aaaaaggcga agtaattgga acaactaaaa cagatgaaaa tggtaaatat cgtttcgata
     3541 atttagatag cggtaaatat aaagttattt ttgaaaagcc tgctggctta acacaaacag
     3601 gtacaaatac aactgaagat gataaagatg cagatggtgg cgaagttgac gtaacaatta
     3661 cggatcatga tgatttcaca cttgataacg gatacttcga agaagataca tcagacagcg
     3721 actcagactc agacagtgat tccgattcag aaagcgactc agactcagat agtgaatcag
     3781 actcagacag tgattcagac tcagacagtg actcagactc agacagtgac tcagactcag
     3841 acagtgattc agactcagat agcgattcag attcagacag tgactcagac tcagacagtg
     3901 actcagattc agatagcgac tcagattcag atagcgactc agactcagat agtgactcag
     3961 actcagacag tgactcagac tcagacagtg actcagattc agacagcgat tcagactcag
     4021 atagcgactc agattcagac agtgactcag actcagatag cgattcagat tcagacagtg
     4081 actcagactc agatagcgat tcagattcag atgcaggaaa acatacacct gtgaaaccga
     4141 tgagtgctac taaagaccat cacaataaag caaaagcatt accagaaaca ggtagtgaaa
     4201 ataacggctc aaataacgca acgttatttg gcggattatt cgcagcatta ggttcattat
     4261 tgttatttgg tcgtcgtaaa aaacagaata agtaatttgc ttaaccaaac caggtccgtg
     4321 aggcctggtt ttttatttca aaatttatga aatgtatctt atgtttgaga ttaaatagtg
     4381 caatatttta aaagatataa catacatcaa cataaatt
//
LOCUS       DATTQF010000174         4194 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.176, whole genome shotgun sequence.
ACCESSION   DATTQF010000174 DATTQF010000000
VERSION     DATTQF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.176"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            256..1713
                     /locus_tag="VB241_002462"
     CDS             256..1713
                     /locus_tag="VB241_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEP1245706.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPAAYAEQDQKWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNDYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGVVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1706..2428
                     /locus_tag="VB241_002463"
     CDS             1706..2428
                     /locus_tag="VB241_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP1245707.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2579..3706
                     /gene="queG"
                     /locus_tag="VB241_002464"
     CDS             2579..3706
                     /gene="queG"
                     /locus_tag="VB241_002464"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP1245708.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3711..4181
                     /gene="trmL"
                     /locus_tag="VB241_002465"
     CDS             3711..4181
                     /gene="trmL"
                     /locus_tag="VB241_002465"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEP1245709.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
BASE COUNT         1533 a          554 c          811 g         1296 t
ORIGIN      
        1 atagcttgat gacaagaata atttttaata tatgataaat ataggcgtta ctgtcaataa
       61 gatattgata gtagcgcttt ttttgattga ttttaaaatt taagaattgt tcgcgtataa
      121 tagacgattt tttaagtaaa gtcttttaaa aagaagcttt tctttatatt gataaaaata
      181 catttataaa acactattgc atcaaaatgt attaatatgt ataattatga atattaatta
      241 gggagggctg tataaatgaa gtgtttaatt aggtttatat tagtattagg gttgttaatt
      301 agcagtgcta tggtgtatat caatcccgca gcatatgctg aacaagatca aaaatgggag
      361 aagattaaag aacgcggtga acttagagtg ggtctttctg cagattatgc accaatggaa
      421 tttgagcata cagttaatgg taagactgag tatgcaggtg tagatattga tttagctaaa
      481 aaaattgcga aagataataa tttaaaatta aaaatcgtca atatgtcatt tgatagtttg
      541 ttaggagctc ttaaaactgg aaaaattgat attattattt ccggaatgac ttcaacgcct
      601 gaacgtaaga agcaagttga tttttcagat tcatatatga tgactaaaaa tatcatgctt
      661 gtaaagaaag ataaagttaa tgattataaa gatatcaaag actttaataa taaaaaagta
      721 ggggcacaaa agggaactga acaagaaaaa atcgctcaaa ccgaaattga aaatgcatct
      781 attacttcat taagccgttt accagacgtt atattagctc tgaaaagtgg aaaggttgaa
      841 ggtgttgtag ttgaaaaacc tgtagcagaa gcatatttaa aacaaaatcc taaattagga
      901 atttccaatg tgaaatttaa tgaagaagaa aaagatacag tgatagcagt accaaaagat
      961 tcaccaaaat tattgtcaca aattaataaa acgattaaag aggttaaaga taaaggatta
     1021 atcgataaat atatgactaa tgctgcaaat gcgatgaatg atgacagtgg ttttatttct
     1081 aagtatggta gttttttctt gaaaggaatt aagattacaa tattaatttc acttatcggt
     1141 gtagcattag gttctatttt aggtgcattc gttgcgttaa tgaaattaag taaaataaaa
     1201 attatttcat ggattgcctc tatctatatc gaaatattaa gaggaacacc aatgttagtt
     1261 caagtattta tcgtattctt tggtataacg gctgcattag gactagacat ttcagctctt
     1321 gtttgtggaa caattgcatt agttattaat tcctcagctt acattgctga aattattcgt
     1381 gcaggtataa atgctgttga taaaggccaa atggaagccg cacgtagtct aggtttgaat
     1441 tatagacaaa cgatgaaaag tgtaattatg ccacaagcaa ttaaaaacat tttaccagct
     1501 ttaggtaatg aatttgtcac tttaattaaa gaatcatcta ttgtgtcaac aattggagtt
     1561 ggcgaaatta tgtttaatgc acaagtggtt caaggtattt catttgaccc attcacacca
     1621 ttactagtgg cagcagcatt atactttgtc ttaacatttg tacttacacg cattatgaac
     1681 atgattgaag ggagattgaa tgccagtgat taaaataaac aatcttaata aagtttttgg
     1741 agataatgaa gttttaaaag atatcaatct tgaaatcaat caaggggaag tagtagcaat
     1801 aataggtcca tctggtagtg gtaaaagtac attgttaaga tgtatgaatt tattagaagt
     1861 acccactaaa ggtcaagtga tttttgaagg caatgattta acggaaaaag ggacacaagt
     1921 agataaacta cgccaaaaaa tgggtatggt atttcaaaac ttcaacctat ttccacataa
     1981 aaaagttgtc gataatatta ttttagctcc taaattatta aagaaagata ataacgatga
     2041 attacataag gaagcattgt cgttattaga taaagtggga ttaaaagaaa aagcagatgt
     2101 atatccgaat caattatcag gtggtcaaaa acaaagggta gcaattgcaa gagctttagc
     2161 aatgcatcca gatgttattt tattcgatga accaacttca gcattagatc ctgaggtagt
     2221 tggtgatgta ttaaaagtaa tgaaagacct agctaaagaa ggtatgacca tggtggttgt
     2281 gacacatgaa atgggatttg ccaaagatgt aagtgacaaa gtcatattta tggcagatgg
     2341 cgttgtcgtc gagtcaggca caccagtcga aatatttgaa caaccgcaac atgaaagaac
     2401 acaaaatttc ttagcaagag tattataaca acctaacgta gcttgaatat atgatgcgca
     2461 ccacaaagtt atatcatata ttcaagcttt ttattattaa aattatgtga aatgaaacct
     2521 caatatgaat aatggttgtg ttaaaataac atagtattat tttatggggg gcatacgatt
     2581 ggatacaaag cagttaaagc aagatatcat tgactatgca tatacaattg gtatcgacag
     2641 tattggattt actactgccg atccctttga tgaattgaag caaaagctag aagcatatca
     2701 tgcaaatggt tatgcctcag gatttgaaga atctgatatt gctttacgaa cggagcctaa
     2761 attatcctta ccaacagcaa ggtcaatcat agcaattgca gttggttatc ctaacaaact
     2821 gaaaggtgca cctaagagtg ttagaggtga tcgcagaggc ttatttgcta gagcatcgtg
     2881 gggtcaagat tatcatacaa ttatgcgtaa acggttagat atgttagctg catttattga
     2941 atctaaagtt ccagatgttg aaatcaaatc tatggtagat acgggtgtat tatcagatag
     3001 ggcagtagca gaacgtgcag gtttaggatt tgttggtcgt aatggctttg tcatcaatcc
     3061 taaactagga acatggacat acctcggtga aatgttagtc agtatacctt ttgaacctga
     3121 tgatccatta ttagatagct gtggtgattg tacaatttgt gttgatcgtt gtccaacaag
     3181 tgcattagtt ggaaatggcc aattaaatag tcagaaatgc attagttttt taacgcaaac
     3241 aaaaggctat atgcctgacc aatatcgtta taaaattggg aatagattat atggttgcga
     3301 tacgtgtcaa caagtttgcc cgaaaaatag aggtattaat accgaacaag atgacatcat
     3361 tttggaacca gaaattttaa agccaagatt agtaccttta ctacgtatgt ctaataaaga
     3421 attcaaacaa acatatggtc accttgcagg tgcttggcgt ggtaaaaaac cgatacaacg
     3481 aaatgctatt ttagcattgg cgcattttaa tgaagtggat gcaattccag aattgaaaaa
     3541 agttgcaaca acagatgaaa gaccgatgat tcgagcaact gcttattggg ctattggtca
     3601 aattcttggg gaagaagcaa gagattttat caatgcaaat tacgatcaag aggatgcaga
     3661 agttcagaat gaaatgataa aaggattgga cacaaggaga gaatagtaat atgacaaatc
     3721 atatcgtttt atatcaacca gaaatcccag ctaatactgg taacattgca cgtacgtgtg
     3781 caggtacaaa cacacattta catttaatta aacctcttgg atttagaact gatgataaaa
     3841 tgttaaaaag agccggttta gattattggg aatttgtgaa tattacgtat catgatagta
     3901 ttgaagcatt ttttgaagcc acaaatggcg agtattactt attaacaaaa tttggtaaaa
     3961 agacttatag tgattttgat ttttcaaatc atgacaaaga tttttacttc atttttggaa
     4021 aagaaacgac aggattacca gactgggtga aagagaaata tcaagacaca gcgttaagaa
     4081 ttcctatgag tgaacatatt cgttcattaa atttatcaaa tactgctgcg ttattaattt
     4141 atgaagcgtt acgtcaacaa gattttccag gattgaatta aaatttactc attt
//
LOCUS       DATTQF010000175         4185 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.177, whole genome shotgun sequence.
ACCESSION   DATTQF010000175 DATTQF010000000
VERSION     DATTQF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.177"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            189..905
                     /locus_tag="VB241_002466"
     CDS             189..905
                     /locus_tag="VB241_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEP1245710.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNTQGIN
                     DDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            898..1923
                     /locus_tag="VB241_002467"
     CDS             898..1923
                     /locus_tag="VB241_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEP1245711.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            1945..3639
                     /locus_tag="VB241_002468"
     CDS             1945..3639
                     /locus_tag="VB241_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEP1245712.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFLCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIDITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMENELPLIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDYREVNDILFVTDLLISDYSSLIYEYVVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
BASE COUNT         1459 a          630 c          803 g         1293 t
ORIGIN      
        1 aactgcatgg caaaacaact tagaatggtc agttacaaaa atacattttt ataaaaaatt
       61 atcacactat tgtgacaact gcctttggct taataaaaga ggaaagtgag caataggttg
      121 ggcttatgtg tgggcatagg tcagtaatgt ataaatggaa atgatgtaat gacagaatgg
      181 aggacaacat gatttatgca ggtattttag caggaggtat tggttcgaga atgggaaacg
      241 tgccattacc aaaacaattt ttagatattg ataataaacc gattttaatt catacaattg
      301 agaagttcat tttagtgagt gaatttaatg agattattat cgcaacgcca gcacagtgga
      361 tttcgcatac gcaagatatt ttaaaaaaat ataacattac agatcaacgt gtcaaagtag
      421 ttgcaggtgg tacggatcga aacgaaacaa ttatgaacat tatcgaccat attcgcaata
      481 cgcagggtat caacgatgat gatgtgattg taactcatga tgccgtaaga ccatttttaa
      541 ctcaacgtat tattaaagag aacattgaag tagcagcaaa atatggtgca gtagatacag
      601 tcattgaagc aattgatacg attgtaatgt ctaaagataa acagaacata cacagtatcc
      661 ctgtaaggaa tgaaatgtat caaggccaaa caccacaatc atttaatatt aaattattac
      721 aagatagtta tcgtgcctta agtagtgcac aaaaagaaat cttatcagat gcatgtaaaa
      781 tcattgtcga atctggacat gcagttaaat tggtacgcgg agaactatac aacattaaag
      841 tgacaacacc gtatgattta aaagtagcaa atgccattat tcaaggtgat attgccgatg
      901 attaatcaag tatatcaact cgttgcaccg agacagttcg acgtcacata taataatgtt
      961 gatatttatg gtaatcatgt catcgtaaga cctttatact tgtctatttg tgcagctgat
     1021 caaaggtatt acacaggtcg aagagatgaa aatgtactgc gcaaaaaatt accaatgtca
     1081 ttagttcatg aagctgttgg tgaagttgta ttcgatagta aaggcgtatt tgaaaaaggt
     1141 acgaaagtag taatggtgcc gaatacacct acagaaaaac acgacgtgat tgcggagaat
     1201 tacttagcct ctagttattt tagatctagt ggttatgatg gttttatgca agactacgtt
     1261 gtgatggcac atgatcgtat cgttccgctg cctaatgaca ttgatttgag tacgatttca
     1321 tacacagagt tagtgtcagt aagttatcat gctatacaac gatttgaacg taaatctata
     1381 cctttgaaaa ccagctttgg tatttggggt gatggtaact taggttatat tactgctatt
     1441 ttgctgcgta agttgtaccc agaagctaaa atttatgtat ttggtaagac agactataaa
     1501 ttaagtcatt tttcatttgt agatgacatc tttacagtaa atcaaatacc agatgatctt
     1561 aaaattgatc atgcatttga atgtgttgga ggtaaaggaa gtcaagttgc acttcaacaa
     1621 atagttgaac atatttcacc agaaggcagt attgctttgt taggcgtaag tgaattaccc
     1681 gtggaagtga atacacgatt agtacttgaa aaaggattaa cgttgattgg tagtagtcga
     1741 agcggctcta aagattttga gcaagttgtt gatttatatc gtaagtaccc agacattgtt
     1801 gaaaagttag ccttattaaa aggacatgaa attaatgtat gtacgatgca agatatcgtc
     1861 caagcgtttg aaatggattt atcgacatct tggggaaaaa cagtattgaa atggacgatt
     1921 taataaaccg atgggggaat aacaatgaca aaaacgaaac aagcaataca tattgataac
     1981 atatactggg aacgtgttca gttatatatt gaaggacata gtgaaggcgt agatttaaca
     2041 tcaggacaat ttgttctgag gaatttaacc gaaacaaaaa cattagaagc aaatgaaatg
     2101 aaaatagacg gtaatacatt tctatgtaga ttcaatgtcg caatattaga cgatgggtat
     2161 tatttaccaa tggataaata tttatttgtt tatcatgacc agttagagta tattggacaa
     2221 cttaatccaa atattattga tcaagcttat gcggcattaa atgaagagca aattgaagaa
     2281 tacaatgagc tgactacaca aaatggaaaa gcaaactatg tgttagccaa cgaagctaaa
     2341 gttttccgca aaggtggcgt atcacaacat acggtttata ccattactcc agaaatagca
     2401 agtgacgtta acgaatttgt atttgatatt gacatcacct tacctcaaga gaaatcaggg
     2461 gtcattgcga caagcgcaca ctggcttcat aaacaaggcc ataaagcttc atttgaaagt
     2521 agaagtttct tatttaaagc tatttttaat attacaaagt tactacatat taaaagaagc
     2581 aaaacaatat tattcacatc agattcacgt ccgaatttat caggtaactt taaatatgta
     2641 tatgatgagt tactacgcca aaaagtagat tttgattatg atattaaaac ggtatttaag
     2701 gcgaatatta cggatagacg taaatggaga gacaagttta gattgccata tttacttggt
     2761 aaggctgatt atatttttgt cgatgatttc catccattaa tttatacggt tcgctttaga
     2821 ccatcacaag aaattattca agtgtggcat gccgttggtg cttttaaaac agttggcttt
     2881 agtcgtacag gtaaaaaagg tggtccgttt attgattcat taaatcatcg tagctataca
     2941 aaagcttatg tatcatcaga aaccgatatt ccattttatg ctgaagcatt tggaattaga
     3001 gaagaaaatg ttgtaccaac aggtgtacca cgtactgatg tactatttga tgaagcttat
     3061 gcaacacaaa ttaaacaaga gatggaaaat gaattaccac ttataaaagg taagaaagtt
     3121 attctattcg caccgacatt tagaggtaat ggtcacggta cggcacatta tccatttttt
     3181 aaaattgatt ttgaacgttt agcaagatat tgcgagaagc ataatgcagt tgtgttattc
     3241 aaaatgcatc cgttcgtaaa aaatagactc aatatttcac gtgaacatag acaatacttt
     3301 atcgatgtgt cagattatcg tgaagttaac gatattctct ttgttacaga cttgttgatt
     3361 agtgattatt catctttaat atatgaatat gtagtattta aaaaaccaat gattttctat
     3421 gcatttgact tagaagatta tattacgacg cgtgatttct atgaaccata tgaatcattt
     3481 gttccaggta aaattgtaca atcctttgat gcattaatgg atgctttgga caatgaagat
     3541 tatgaggttg aaaaagttgt accattctta gataaacatt ttaaatatca agatggtcgc
     3601 tcaagtgaac gtttagtcaa agatttgttt agacgctaat gttggcatat attacttgcc
     3661 atgcaaatga tcataaggca tagttttcaa gaagggttat gacaatgaag tgagcaacat
     3721 gtcatgatga gaagcaggac tatacaatga gaataacctt ttgatttttc atgcataaga
     3781 ggctgggaca taaatcccta aaaaaacagc agtaagataa ttttcaatta gaaaatatct
     3841 tactgctgtt ctctatttat acaatacttc gtattgaatg gcttcgcttt gcccgtctgg
     3901 cacattactg taaaattcta taaatagaat ttttgatgac gggtcccttc ctagggtgcc
     3961 gtctcagcca ccccaaccgg cacattgttg taagctgact atatgtcagc ttctgtgttg
     4021 gggcccctgt cttcgactgg cactgctccc tcaggagtct cgccattaat actacgtatt
     4081 aacatgtaat tttactttta aatactttga aaaaataaga cactttgccc aacttgcaca
     4141 taaatgtaaa attcaataaa atgaattttc tgtgttaggg cccac
//
LOCUS       DATTQF010000176         4029 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.178, whole genome shotgun sequence.
ACCESSION   DATTQF010000176 DATTQF010000000
VERSION     DATTQF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.178"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1000)
                     /locus_tag="VB241_002469"
     CDS             complement(<1..1000)
                     /locus_tag="VB241_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP1245713.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHEL"
     gene            complement(1547..1630)
                     /locus_tag="VB241_002470"
     tRNA            complement(1547..1630)
                     /locus_tag="VB241_002470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1594..1596),aa:Leu,seq:caa)
     gene            complement(1759..1833)
                     /locus_tag="VB241_002471"
     tRNA            complement(1759..1833)
                     /locus_tag="VB241_002471"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1798..1800),aa:Gly,seq:tcc)
     gene            complement(1841..1914)
                     /locus_tag="VB241_002472"
     tRNA            complement(1841..1914)
                     /locus_tag="VB241_002472"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1880..1882),aa:Cys,seq:gca)
     gene            complement(1920..1991)
                     /locus_tag="VB241_002473"
     tRNA            complement(1920..1991)
                     /locus_tag="VB241_002473"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1957..1959),aa:Gln,seq:ttg)
     gene            complement(2003..2075)
                     /locus_tag="VB241_002474"
     tRNA            complement(2003..2075)
                     /locus_tag="VB241_002474"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2040..2042),aa:His,seq:gtg)
     gene            complement(2078..2151)
                     /locus_tag="VB241_002475"
     tRNA            complement(2078..2151)
                     /locus_tag="VB241_002475"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2117..2119),aa:Trp,seq:cca)
     gene            complement(2167..2247)
                     /locus_tag="VB241_002476"
     tRNA            complement(2167..2247)
                     /locus_tag="VB241_002476"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2211..2213),aa:Tyr,seq:gta)
     gene            complement(2253..2328)
                     /locus_tag="VB241_002477"
     tRNA            complement(2253..2328)
                     /locus_tag="VB241_002477"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2293..2295),aa:Thr,seq:tgt)
     gene            complement(2336..2408)
                     /locus_tag="VB241_002478"
     tRNA            complement(2336..2408)
                     /locus_tag="VB241_002478"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2373..2375),aa:Phe,seq:gaa)
     gene            complement(2427..2502)
                     /locus_tag="VB241_002479"
     tRNA            complement(2427..2502)
                     /locus_tag="VB241_002479"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2467..2469),aa:Asp,seq:gtc)
     gene            complement(2512..2585)
                     /locus_tag="VB241_002480"
     tRNA            complement(2512..2585)
                     /locus_tag="VB241_002480"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2549..2551),aa:Met,seq:cat)
     gene            complement(2600..2692)
                     /locus_tag="VB241_002481"
     tRNA            complement(2600..2692)
                     /locus_tag="VB241_002481"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2654..2656),aa:Ser,seq:tga)
     gene            complement(2731..2806)
                     /locus_tag="VB241_002482"
     tRNA            complement(2731..2806)
                     /locus_tag="VB241_002482"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2771..2773),aa:Asp,seq:gtc)
     gene            complement(2817..2906)
                     /locus_tag="VB241_002483"
     tRNA            complement(2817..2906)
                     /locus_tag="VB241_002483"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2868..2870),aa:Ser,seq:tga)
     gene            complement(2916..2989)
                     /locus_tag="VB241_002484"
     tRNA            complement(2916..2989)
                     /locus_tag="VB241_002484"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2953..2955),aa:Ile,seq:cat)
     gene            complement(3018..3091)
                     /locus_tag="VB241_002485"
     tRNA            complement(3018..3091)
                     /locus_tag="VB241_002485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3055..3057),aa:Met,seq:cat)
     gene            complement(3113..3188)
                     /locus_tag="VB241_002486"
     tRNA            complement(3113..3188)
                     /locus_tag="VB241_002486"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3153..3155),aa:Ala,seq:tgc)
     gene            complement(3206..3279)
                     /locus_tag="VB241_002487"
     tRNA            complement(3206..3279)
                     /locus_tag="VB241_002487"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3243..3245),aa:Pro,seq:tgg)
     gene            complement(3289..3362)
                     /locus_tag="VB241_002488"
     tRNA            complement(3289..3362)
                     /locus_tag="VB241_002488"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3326..3328),aa:Arg,seq:acg)
     gene            complement(3381..3469)
                     /locus_tag="VB241_002489"
     tRNA            complement(3381..3469)
                     /locus_tag="VB241_002489"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3433..3435),aa:Leu,seq:taa)
     gene            complement(3480..3554)
                     /locus_tag="VB241_002490"
     tRNA            complement(3480..3554)
                     /locus_tag="VB241_002490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3520..3522),aa:Gly,seq:gcc)
     gene            complement(3558..3639)
                     /locus_tag="VB241_002491"
     tRNA            complement(3558..3639)
                     /locus_tag="VB241_002491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3603..3605),aa:Leu,seq:tag)
     gene            complement(3643..3718)
                     /locus_tag="VB241_002492"
     tRNA            complement(3643..3718)
                     /locus_tag="VB241_002492"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3683..3685),aa:Lys,seq:ttt)
     gene            complement(3724..3799)
                     /locus_tag="VB241_002493"
     tRNA            complement(3724..3799)
                     /locus_tag="VB241_002493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3764..3766),aa:Thr,seq:tgt)
     gene            complement(3816..3891)
                     /locus_tag="VB241_002494"
     tRNA            complement(3816..3891)
                     /locus_tag="VB241_002494"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3856..3858),aa:Val,seq:tac)
     gene            complement(3903..4017)
                     /gene="rrf"
                     /locus_tag="VB241_002495"
     rRNA            complement(3903..4017)
                     /gene="rrf"
                     /locus_tag="VB241_002495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1282 a          916 c          792 g         1039 t
ORIGIN      
        1 acaattcatg aacaataaac gccgatacaa atggtatgac aaaataacca agagctaata
       61 aaataatatt cattgttgga tcacctgaca ttcggttaaa tgcatttatt gggccaacta
      121 atcctgtata tccaaatcca gcagacaatg gtgttccttt aacctgaaga acgtaagcaa
      181 tgattccagt aattattcca tttatagtca atggtatcga aataattaaa tttttcaagt
      241 aaactgggat catcattttt gcagctccta tgagtaaaac tgcatttacg ccaatagaat
      301 tgacacgcaa tgagccgaat aagaaagtaa cacaagcagc cactatacct aggtttgctg
      361 ctccacttcc tagcccgttt aaactaatcg cagttgcaat cgctactaac gatattggtg
      421 ttaccattaa taatgaaaat gccacactaa taagtataga cattaacaac ggatttaagt
      481 ctgtaaaaga atgaattaca tttccaattg cttgagtaat ttttcgaatg taaggtaatg
      541 tgattagacc gataccccca ctaacgatag gtactaaaac tggtaatata attaattcaa
      601 aagatccaag cttgttttgt aataccatat atataagaca tgcaataata acaaccaaac
      661 ttgtatttat aatgtcacct atacctttta acataaaact attattgcta tatacaacag
      721 cacctgaacc aatcatagct gatgtaccta ctatagcagc acctgcgcca ttaaatttaa
      781 attgatgagc agctaaaacc ccaataataa atgccataaa tgattgaatt agtatcacta
      841 actgatacgt taattctaaa atttcattac cacttttaaa tatttttaat acttcaccta
      901 ataaagcatt cggaacaagt gcaataacaa caccagcacc aatagaattt aaaatcttac
      961 tgaaaaactg tttattatca gcattctttg cgttactcat aaacgacctc caatttgaac
     1021 attaaactca tcatattata gaaaattaac tttaacaatg attagttggc taatttaatc
     1081 taaatataac aattcacaaa aggaataaag taaattggaa caatctaatc agtaaacaat
     1141 gttatgttaa ttttaaaata ctactaatta catcttgtga ctaataatgt aataaattgt
     1201 aaatattaaa atagccatat tactattaag atagataaat tgaatcgact tggtataaag
     1261 cacaataaaa tagcactttt acaaatattt tattagaaaa gttaaaacaa atacataccc
     1321 cagataatat agtcattatc attaatgtaa taccaaaaaa agaagttaaa caatgtatag
     1381 ttgcttaact tcctatatta aactcatcat tacaatttga catagagcct attaaagcgt
     1441 gtgccatttg agtgccactt tatttgtatt gtatagagag aaataaaaag aaaccttgtt
     1501 ttacaaggtt tctagtacgt tatgttatgt aaataacagt taattatacc ggtggtcggg
     1561 gtcgaaccga cactccacaa gtggaacggg attttgagtc ccgcgcgtct gccaattccg
     1621 ccacaccggc ttaatggtaa acaaaaaact tccctttgga agcaattatg gagcggaaga
     1681 taggatttac acctatacct cattccagga aggaatgtat tctaagagtt gaaatactcc
     1741 cgcattatta ttaaattatg gagcggaaga taggatttgc acctatacct cgttccggga
     1801 aggaacgtgt tctaaaagtt gaactactcc cgcataaacc tggaggcggc aaccggattt
     1861 gaaccggtga taaaggtttt gcagacctct gccttaccac ttggctatgc cgccaataac
     1921 tgggctagct ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg
     1981 gctatagccc attaataata agggcggctg aaggggatcg aaccctcgaa tgtcggaacc
     2041 acaatccgat gtgttaacca cttcaccaca gccgccatgg caggggcagt aggaatcgaa
     2101 cccacaccaa aggttttgga gacctctatt ctaccgttga actatgcccc tattaaaaat
     2161 aataaatgga ggggggcaga ttcgaactgc cgaacccgaa ggagcggatt tacagtccgc
     2221 cgcgtttagc cacttcgcta cccctccata aatggtgccg gccagaggac ttgaaccccc
     2281 aacctactga ttacaagtca gttgctctac caattgagct aggccggcta agaaatggtt
     2341 caggacagag tcgaactgcc gacacatgga gcttcaatcc attgctctac caactgagct
     2401 actgaaccat aataaaaatg taatgatggc ggtctcgacg ggaatcgaac ccgcgatctc
     2461 ctgcgtgaca ggcaggcgtg ttaaccgcta cactacgaga cctataaaat attgcgggag
     2521 gcggatttga accaccgacc ttcgggttat gagcccgacg agctaccgaa ctgctccatc
     2581 ccgcgataat aaaaaataat ggcggaggaa gagggattcg aacccccgcg gcccgttaag
     2641 gccctgtcgg ttttcaagac cgatcccttc agccggactt gggtattcct ccattattat
     2701 aggtaaatcg ctattaatta taaaattaaa tggcggtctc gacgggaatc gaacccgcga
     2761 tctcctgcgt gacaggcagg cgtgttaacc gctacactac gagaccatta gtaaaacgga
     2821 ggaagaggga ttcgaacccc cgcgagccgt taagcccctg tcggttttca agaccgatcc
     2881 cttcagccgg acttgggtat tcctccaaaa ttatatggac cttgcaggac tcgaacctgc
     2941 gaccgaacgg ttatgagccg ttagctctaa ccaactgagc taaaggtcct aaatataatt
     3001 ttacaactaa taaatagtgg cggtggaggg gatcgaaccc ccgacctcac gggtatgaac
     3061 cgtacgctct agccagctga gctacaccgc cttatatagt ttgtaaataa tatggtggag
     3121 actagcggga tcgaaccgct gacctcctgc gtgcaaagca ggcgctctcc cagctgagct
     3181 aagcccccat aataattaca gtatatcggg aagacaggat tcgaacctgc gaccccttgg
     3241 tcccaaacca agtgctctac caagctgagc tacttcccgt ataattaacg cgcccgatag
     3301 gagtcgaacc cataacctct tgatccgtag tcaaacgctc tatccaattg agctacgggc
     3361 gcatatattt ttattgaaaa tggtgccgag gaccggaatc gaaccggtac ggtgatcact
     3421 caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc caccccggca ctataaaaat
     3481 ggagcagaag acgggattcg aacccgcgac cccaaccttg gcaaggttgt attctaccgc
     3541 tgaactactt ctgcatatgc gggtgaaggg agtcgaaccc ccacgccgta aggcgctaga
     3601 tcctaagtct agtgcgtctg ccaattccgc cacacccgca aatggtgagc catagaggat
     3661 tcgaacctct gaccctctga ttaaaagtca gatgctctac caactgagct aatggctctt
     3721 ccatggtgcc ggccagagga cttgaacccc caacctactg attacaagtc agttgctcta
     3781 ccaattgagc taggccggca atatgtaaga ataaatggtg gagaatgacg ggttcgaacc
     3841 gccgaccctc tgcttgtaag gcagatgctc tcccagctga gctaattctc cgatttaaaa
     3901 ctgcctggca acgttctact ctagcggaac gtaagttcga ctaccatcga cgctaaggag
     3961 cttaacttct gtgttcggca tgggaacagg tgtgacctcc ttgctatagt caccagacat
     4021 atgaatgta
//
LOCUS       DATTQF010000177         3970 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.179, whole genome shotgun sequence.
ACCESSION   DATTQF010000177 DATTQF010000000
VERSION     DATTQF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.179"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..851
                     /gene="ylqF"
                     /locus_tag="VB241_002496"
     CDS             <1..851
                     /gene="ylqF"
                     /locus_tag="VB241_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP1245714.1"
                     /translation="AKREVSEQLKKVDVVFELVDARIPYSSRNPMIDEVINQKPRVVI
                     LNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIKATAEKFEREKAK
                     GLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQWIKVGNALQLLDT
                     PGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDLARLKAHYKIEVP
                     EDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNYCFDIFKDMTEEL
                     ANDANN"
     gene            835..1602
                     /locus_tag="VB241_002497"
     CDS             835..1602
                     /locus_tag="VB241_002497"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP1245715.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILTENPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1711..2877
                     /gene="sucC"
                     /locus_tag="VB241_002498"
     CDS             1711..2877
                     /gene="sucC"
                     /locus_tag="VB241_002498"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEP1245716.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            2899..3807
                     /gene="sucD"
                     /locus_tag="VB241_002499"
     CDS             2899..3807
                     /gene="sucD"
                     /locus_tag="VB241_002499"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEP1245717.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYELLLTVNK"
BASE COUNT         1455 a          543 c          848 g         1124 t
ORIGIN      
        1 aagccaaaag agaagtaagt gaacaattaa aaaaagtaga tgtagtgttt gaactagtag
       61 atgcaagaat tccatatagt tcaagaaacc ctatgataga tgaagttatt aaccaaaaac
      121 cacgtgttgt tatattaaat aaaaaagata tgtctaattt aaatgagatg tcaaaatggg
      181 aacaattttt tattgataaa ggatactatc ctgtatcagt ggatgctaag cacggtaaaa
      241 atttaaagaa agtggaagct gcagcaatta aggcgactgc tgaaaaattt gaacgtgaaa
      301 aagcaaaagg acttaagcca agagcgatta gagcgatgat tgttggaatt ccaaatgttg
      361 gtaaatccac attaataaat aaactggcaa agcgtagtat tgcacagact ggtaataaac
      421 caggtgtgac caaacaacaa caatggatta aagttggtaa tgcattacaa ctattagata
      481 caccagggat tctttggcct aaatttgaag atgaagaggt cggtaagaag ttgagtttaa
      541 caggcgcgat taaagatagt attgtgcact tagatgaagt tgccatctat ggactgaact
      601 ttttaattca aaatgattta gcgcgcttaa aagcacatta taaaatcgaa gttcctgaag
      661 atgcagaaat catagcgtgg tttgatgcga tagggaaaaa acgtggctta attcgacgtg
      721 gtaatgaaat tgattacgaa gcagtcattg aactgattat ttatgatatt cgaaatgcta
      781 aaataggaaa ttattgtttt gatattttta aagatatgac tgaggaatta gcaaatgacg
      841 ctaacaatta aagaagtaac gcagttgatt aatgcggtta atacaataga agaattagaa
      901 aatcatgaat gctttttaga tgagcgaaaa ggtgtacaaa atgccatagc cagacgaaga
      961 aaagcgttag aaaaagaaca agctttaaaa gaaaagtatg ttgaaatgac ttactttgaa
     1021 aatgaaatat taacagagaa tcctaatgct attatttgtg gtattgatga agttggaaga
     1081 ggacctttag caggtccagt cgttgcatgc gcaacaattt taaattcaaa tcacaattat
     1141 ttgggccttg atgattcgaa aaaagtacct gttacgaaac gtctagaatt aaatgaagca
     1201 ctaaaaaatg aagttactgc ttttgcatat ggtatcgcga cagctgaaga aatagatgaa
     1261 tttaatattt ataaagctac tcaaatcgcc atgcagcgag ctattgatgg attatcagta
     1321 caaccaacgc atttattgat agacgcgatg acgcttgata atgcactgcc tcaagtatct
     1381 ttaatcaagg gtgatgcaag aagtgtgtct attgcagctg caagcatcat ggcaaaggtt
     1441 tttcgtgatg attatatgac acagttatct aaagattatc ctgaatatgg ttttgaaaaa
     1501 aatgcgggtt acggtaccaa acaacattta ctagcaatcg atgatattgg cattatgaaa
     1561 gagcatagaa aaagctttga acctataaaa tcgttactgt aaatcgtaaa attcaattaa
     1621 atgataaaaa agtagcattt aagtttacaa tagcgcttac attttttata attgtaaggg
     1681 aacttaacct aagtaacagg aggatggaag atgaatatcc acgagtatca aggtaaagaa
     1741 atatttcgtt caatgggcgt tgcagttcca gaaggacgag tagcatttac tgctgaagaa
     1801 gcggtggaga aagcaaaaga attaaattct gatgtatatg ttgtgaaagc acaaattcat
     1861 gctggaggta gaggtaaagc aggcggagta aaaattgcta aatctttatc tgaggtagaa
     1921 acatatgcaa aagaattatt agggaaaact ttggtgacac atcaaactgg tccagaaggt
     1981 aaagaaatta agcgtttata tatcgaagaa ggttgtgcta ttcaaaaaga atattacgtt
     2041 ggattcgtta ttgatcgtgc gactgaccaa gtaacattga tggcgtctga agaagggggc
     2101 actgagattg aagaagttgc tgcgaagact cctgaaaaga tcttcaaaga aactatcgat
     2161 ccagtaatcg gactttcacc attccaagca agacgaattg cgtttaatat taatattcct
     2221 aaagaatctg ttaacaaagc tgctaaattc ttattagcac tttataatgt attcattgaa
     2281 aaagattgtt caatcgtaga aatcaaccca ttagttacaa cagctgatgg tgatgtattg
     2341 gcattagatg ctaaaattaa ttttgatgat aatgcattat tcagacataa agatgttgta
     2401 gaattacgtg atttagaaga agaagatccg aaagagattg aagcgtctaa acatgattta
     2461 tcatacattg cattagatgg tgacatcgga tgtatggtta atggtgcagg tttagccatg
     2521 gcaacaatgg atacgattaa tcatttcggt ggaaacccag ccaatttctt agatgcaggc
     2581 ggaagcgcta ctagagaaaa agtaactgaa gcatttaaaa tcattttagg tgatgaaaat
     2641 gttaaaggta tttttgtaaa cattttcggt ggcattatga aatgtgatgt tatcgcagaa
     2701 ggtatcgttg aagctgtaaa agaagtagat ttaactttac cactagttgt acgcttagaa
     2761 ggtacaaatg ttgagttagg taaaaaaatc ttaaaagact caggattagc aattgaacca
     2821 gcagcaacaa tggctgaagg tgcacaaaaa attgttaaac tagtcaaaga agcataagga
     2881 aaggatggga gcactaagat gagtgtattt atagataaga atactaaagt aatggtacaa
     2941 ggtattacag ggtctactgc ccttttccat acaaaacaaa tgcttgatta tggtacgaaa
     3001 atagtagcag gtgtgacgcc tggtaaaggt ggtcaagttg ttgaaggcgt tcctgttttc
     3061 aacactgttg aagaagctaa aaatgaaact ggggcaacgg tttcagtcat ttacgttcca
     3121 gcaccatttg ctgcagactc aattttagaa gcagctgatg cagacttaga tatggttatt
     3181 tgtatcactg aacatattcc tgtattagac atggttaaag ttaaacgcta tttacaaggt
     3241 agaaaaacac gtttagttgg tccaaactgt ccaggtgtga ttacagcaga tgaatgtaaa
     3301 attggtatta tgcctggcta tattcacaaa aaaggtcatg ttggtgtagt atcacgttca
     3361 ggtacactaa catatgaagc agtgcaccaa ttgactgaag aaggtattgg tcaaactaca
     3421 gctgttggta ttggtggaga cccagtaaat ggcacaaact ttattgatgt tttaaaagca
     3481 ttcaatgaag atgacgaaac gaaagcagtt gttatgattg gtgaaatcgg tggtacagct
     3541 gaagaagaag cagctgaatg gattaaagcg aatatgacaa aaccagttgt aggctttatc
     3601 ggtggacaaa cagcacctcc tggaaaacgt atgggacatg ctggtgcgat catttcaggt
     3661 ggtaaaggta ctgctgaaga gaaaattaaa acattaaata gttgtggtgt gaaaacagcg
     3721 gcaacacctt cagaaattgg ttcaacatta attgaagctg ctaaagaagc aggtatttat
     3781 gaattattat taacagttaa taaataaagt taaaagatga tataaatggc tatagcccat
     3841 tccatacttt ataaaagtat cggaaatggg ctatttgtcg tgcgatgaat ttaagtacac
     3901 ttttcatatt ttgaaaaggc aagttaatta ttttacctcc ttcaatagct ttctaaaaca
     3961 aagcaaattt
//
LOCUS       DATTQF010000178         3962 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.180, whole genome shotgun sequence.
ACCESSION   DATTQF010000178 DATTQF010000000
VERSION     DATTQF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.180"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            103..828
                     /locus_tag="VB241_002500"
                     /pseudo
     CDS             103..828
                     /locus_tag="VB241_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            1211..1909
                     /locus_tag="VB241_002501"
     CDS             1211..1909
                     /locus_tag="VB241_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HEP1245718.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASEQSRLSVTSNDTQ
                     ELKKYYSGTGYNFQNVSGYREKDKMNIIDGTQLNVVTLLGTDKERFKDYDYDYEGLDV
                     FVVREGSGKQAENISIGGITKTNKNDYKDFVNNVGLEITKPTGHNTATRQAETYRINK
                     EEISLKELDFKLRKHLIENHELYKTEPKDGKIRITMKGGGYYTFELNKKLQPHRMGDV
                     IDGRNIEKIEVDLY"
     gene            1928..3415
                     /locus_tag="VB241_002502"
     CDS             1928..3415
                     /locus_tag="VB241_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HEP1245719.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASEANEKPSTNQE
                     SKVVSQTEQNSKETKTVESNKNFVKLDTIKPGAQKITGTTLPNHYVLLTVDGKSADSV
                     ENGGLGFVEANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGEDEEDEEVEEASTDKAG
                     VEEESTEAKVTYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALSINNKFINFEESVKGGVSKEDTKASSDGIWMPIDDKGYFNFDFKTKRFDNLELKE
                     GNDISLTFAPDDEEGALKPLIFKTKVTSLEDIDKAETKYDHTKLNKVKVLDNVKEDLH
                     VDEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEAQLFPDLQVNEKG
                     EFSFDSHGAGFRLQNGEKLNFTVVDPITGDLLSNEFVSKEIDIEETPEQKADREFDEK
                     LENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERTTPKLEK"
     gene            complement(3521..3829)
                     /gene="spn"
                     /locus_tag="VB241_002503"
     CDS             complement(3521..3829)
                     /gene="spn"
                     /locus_tag="VB241_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HEP1245720.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNEADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1597 a          491 c          709 g         1165 t
ORIGIN      
        1 agatatgtct tttttactaa ttcatttaga aaagaaatga ttacaaaaag ttctatgaca
       61 actagagctt taacatcagg tagcgcaatt aataaaatga aggtcatata ccctgtttcg
      121 gctaaagaac agagaaaaat aggtgacttc ttcagcaaac tcgaccgaca aattgaatta
      181 gaagaacaaa agcttgaatt acttcaacaa caaaaaaaag gctatatgca gaaaatcttc
      241 tcacaggaac tgcgattcaa agatgagaat agtgaagatt atccacattg ggaaaatagc
      301 aaaatagaaa aatatttaaa agagagaaac gaacgttctg acaaaggtca aatgctttca
      361 gtaactataa atagtggcat tataaaattt agtgaattgg atagaaaaga taattcaagt
      421 aaagataaaa gtaattataa agtagttagg aaaaatgata ttgcatataa ttctatgaga
      481 atgtggcaag gggctagtgg tagatcaaat tataatggga ttgttagccc tgcatatact
      541 gtgctttatc caacacaaaa tactagctca ttatttattg gatataagtt taaaacacat
      601 agaatgattc ataaatttaa aattaattca caaggattaa catcagatac atggaactta
      661 aaatataaac aattaaaaaa tataaatata gatatacctg tattggagga acaagaaaag
      721 ataggtgatt tctttaaaaa aatggatata ttgattagta aacagaaaat aaaaattgaa
      781 atattagaaa aagagaaaca atccttttta caaaagatgt tcttataact ttgataaaca
      841 cataggttgc ataagaataa aatttgtgta atttaacata aaagttataa acataaagta
      901 aattaaaaac gaacattaaa tttaggcact gtgatagcac agtgtctttt ttgtgtcgaa
      961 attgtgtaca gaataagtag ttaaataaag attaagttga gataaagtgt tattcgtaaa
     1021 taaaagagag tagatcgata ggaattgaat gatattagtt aactatttat taaatcactt
     1081 aataatgatt aatttttagt taaagtaagt ttaatgtgaa gcacgaccgt tgctcattat
     1141 aatgaatgag gattgttcgt attgcgtaat agaataaatc aaatagacta aaaattggga
     1201 gcatagaatt atgaaattaa aaaatattgc taaagcaagt ttagcactag ggattttgac
     1261 aacagggatg attacaacta ctgctcagcc agtaaaagca agtgagcaaa gcagattatc
     1321 agttacttca aacgacacgc aagaattaaa aaaatactac agtggaacag gatataattt
     1381 tcaaaatgtg agtggttata gagaaaagga taaaatgaac attattgatg ggacacaact
     1441 taatgtagtt actttacttg gtacagacaa agaaagattt aaagattatg actatgatta
     1501 tgaagggtta gatgtctttg tagtcagaga aggatcaggt aaacaagctg aaaatatttc
     1561 aataggtgga attaccaaga cgaataaaaa cgattataaa gatttcgtaa ataatgtagg
     1621 tctagaaata actaaaccaa caggacataa tacagcaaca agacaagcag aaacttatag
     1681 aattaataaa gaagaaattt cattaaaaga attagatttc aaattaagaa aacatttaat
     1741 tgaaaatcat gaactttata agacagagcc taaagacggt aaaattagaa ttactatgaa
     1801 aggtggcggc tactatactt ttgaattaaa taaaaaatta cagcctcatc gtatgggtga
     1861 tgtaattgat ggtagaaata tagaaaaaat tgaagtcgat ttatattaat attcgaggga
     1921 gtatatcatg agggaaaatt ttaagttacg taaaatgaaa gtcgggttag tatctgttgc
     1981 aattacaatg ttatatatca tgacaaacgg acaagcagaa gcatcagagg ctaatgagaa
     2041 gccaagtaca aatcaagaat caaaagttgt ttcacagact gaacaaaatt caaaagaaac
     2101 aaaaacagta gaatctaata agaactttgt taaattagat actattaaac ctggagctca
     2161 aaagataacg ggaactactt taccaaatca ctatgtttta ttaacagttg atgggaaaag
     2221 tgcggattca gtagaaaatg gcggtttggg ttttgttgaa gcaaatgaca aaggagaatt
     2281 tgagtaccct ttaaataatc gtaaaattgt tcataatcaa gaaattgagg tttcgtcgtc
     2341 aagccctgat ttaggtgaag atgaagaaga tgaagaggtg gaagaagctt caactgataa
     2401 agctggcgtt gaggaagaaa gtacagaagc taaagttact tacacaacac cgcgatatga
     2461 aaaagcgtat gaaataccga aagaacaact aaaagaaaaa gatggacatc accaagtttt
     2521 tatcgaacct attactgaag gatcaggtat tattaaaggg catacgtctg taaaaggtaa
     2581 agttgcttta tctattaata ataaatttat taattttgaa gagagcgtta agggcggagt
     2641 tagtaaagaa gacactaaag ctagttcaga tggtatctgg atgcctattg atgacaaagg
     2701 atactttaac tttgacttca aaacgaaacg tttcgataat ttagagttaa aagaaggtaa
     2761 tgacatttca ctaacatttg cacctgatga tgaagaaggt gcattaaaac ctttaatttt
     2821 caaaactaaa gtaacgagct tagaagatat cgataaagca gaaactaaat atgaccatac
     2881 taaactcaac aaagtgaaag ttttagataa tgttaaagaa gatttacatg ttgatgaaat
     2941 atatggaagc ttatatcata cagacaaagg taaaggtatt cttgataaag aaggtactaa
     3001 agtaattaaa ggaaagacta aattcgcgaa tgcagtagtg aaggtagact ctgaactagg
     3061 tgaagcacaa ttattccctg atttacaagt aaatgaaaaa ggtgaattta gctttgactc
     3121 acatggtgct ggttttagat tacaaaatgg agaaaaatta aacttcacag tggttgatcc
     3181 tattacaggt gacttgttaa gtaatgagtt tgtttctaaa gagattgata ttgaagaaac
     3241 acctgaacaa aaagcggatc gtgagtttga cgaaaaactt gaaaatacgc ctgcttacta
     3301 caagttatac ggcgataaaa tagttggatt cgatactaac gatttcccga ttacttggtt
     3361 ctatccattg ggtgaaaaga aagttgaacg tacaacacct aaattagaaa aataattaaa
     3421 taaaacagct taatgatgta atgaaattag tgaattaatc actgacttct acgtcattga
     3481 gctgtttttt tatgtgcttt gttataaagc attattgaat ttattttacg tgttcatatt
     3541 ttgaaacatc aaagccgtct tgcttagctt tgttgataat gtctttgatt gaatgtagtc
     3601 ctttatcggc gaagtatgat cttaagttgt cttttgtagc ttggtcagca ttcttatcta
     3661 ataacacatc gatatagctt aattcatgtt ctaagaagtt tgcatcatca tgtagtacga
     3721 gtccattttg agaatacact ttcgcatctg cttcattacc atatccaaca acgccagttg
     3781 ctaatacacc taccattgcc gtagctacta aaaccttttt aaatttcata tctatcactc
     3841 ctctaaaaat tgtaactcca tcataacact gaatattgag aaaattacgt ttattaagtc
     3901 gatttaatga tttttaataa atagttaaag tgacaaatat tgtttaaatg caattaatct
     3961 tt
//
LOCUS       DATTQF010000179         3941 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.181, whole genome shotgun sequence.
ACCESSION   DATTQF010000179 DATTQF010000000
VERSION     DATTQF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.181"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            228..854
                     /locus_tag="VB241_002504"
     CDS             228..854
                     /locus_tag="VB241_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP1245721.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYENQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            1215..2000
                     /locus_tag="VB241_002505"
     CDS             1215..2000
                     /locus_tag="VB241_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HEP1245722.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     KLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(2603..2737)
                     /locus_tag="VB241_002506"
     CDS             complement(2603..2737)
                     /locus_tag="VB241_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HEP1245723.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            2973..3536
                     /locus_tag="VB241_002507"
     CDS             2973..3536
                     /locus_tag="VB241_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HEP1245724.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTLAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALLSVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
     gene            3540..3680
                     /locus_tag="VB241_002508"
     CDS             3540..3680
                     /locus_tag="VB241_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HEP1245725.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
BASE COUNT         1406 a          545 c          592 g         1398 t
ORIGIN      
        1 gtaacatttt cttaaaccaa tgatatgaca tttcacgaga cacctccctt tttcatataa
       61 gtattaatct aagtataata aatatgcgtt gaaaatgcat ttatatttgg aatttgaaaa
      121 tgaatattaa tattcttgaa attttcgtaa atattacttt acaagttaga gtataaattt
      181 tatactaagt ctattaagta ttctttacta aagaaggagt aatgattatg ggtttattta
      241 aaaaagataa aaaagcaatg acttttgaca acgcaatgga agaaagacgt tcaatatata
      301 atttgaaaga ttcaatatca attagcgatg acgaattaga atcaatgatt gcacatgcag
      361 ttaaacatgt tccttcatca tttaattctc aatcaactag aattgttctc ttattaaatg
      421 acaacaataa taaattttgg gataacacaa aagcaatttt aaaagaagtc atgggagaga
      481 atcgtgattt cgagccaact gaacaaaaaa ttgataactt caaacattca tacggtacaa
      541 ttttgtttta tgaaaatcaa gatgttgtaa gtggtttaca agaacaaatg ccaaactact
      601 atgataattt tgcaatatgg tcaactcaaa caaatgccat gcatcaattt gcaatttgga
      661 ctgcattagc tactaaaggc ataggtgctt cattacaaca ctataatcca cttgttgatg
      721 agatgacttc aaatgaattt aatattccta aaagttggaa acttattgct caaatgccat
      781 ttggtgatat tcgtgaagct gctggcgaaa aaacatttaa tccagtggaa gatcgtttcg
      841 taataaaaaa ataatcaata tattgttggg aatgggacag aaatgatata gaatcattag
      901 ttattcatta tgtagtagtt cttacacatt agccgctgct aatgtgtact taagaatagg
      961 aatacatgag taaaactcat gtataagaaa tactaatttc taaagaaaaa gtatttcttt
     1021 acgttagagc ccaccccaac ttgcattgtc tgtagaaatt ggtgttccaa tttctctatg
     1081 ttggggcccc atcaactact gtcaatataa cattgtagag cctaggactt tgattttatg
     1141 tcctgggctt gttttttgcg tttcaacata acttttagta tgttatatat aagatctatc
     1201 aaggatgtga tgttatgaaa gtccaaattt atcaattacc gattattttt ggagatagtt
     1261 ctaaaaatga aactcaaata acgcaatggt tcgaaaaaaa tatgaatgct gaagtagatg
     1321 tagtcgtttt acctgaaatg tggaataatg gctatgattt agagcattta aatgaaaaag
     1381 cagataataa tctaggacaa agtttttcat ttattaaaca tttagctgaa aaatataaag
     1441 tcgatatcgt agctgggtca gtttctaaca ttagaaacaa tcaaatattt aatacagctt
     1501 ttagtgtgaa taaaagtggc aaactgatta atgaatacga caaagttcat ttagttccta
     1561 tgttaagaga gcatgaattt ttaaccgctg gcgaaaacgt agctgaaccc tttcaattgt
     1621 ctgacggtac ttatgtgacc caacttatct gttatgattt acgttttcca gaattattaa
     1681 gatatcctgc tcgtagtggt gctaaaattg cattttatgt ggcacaatgg cctatgtcac
     1741 gtcttcaaca ttggcattct ttactaaagg cacgtgcaat tgaaaataat atgtttgtca
     1801 ttggaactaa tagcactgga ttcgatggta acacagaata tgctggtcat tcgatagtta
     1861 taaatccaaa cggagattta gttggagaat taaatgaatc cgcagatatt ttgactgtag
     1921 atttaaactt aaatgaagta gaacagcaac gcgaaaatat acctgtattt aaaagtatta
     1981 aattagattt gtataaataa aaagcacacg aaataatatt taacacatgt catgactaaa
     2041 catagattta tgagacgatt tttcataagc ctatgtttat ttttttataa tagataactc
     2101 agtaagaacc agtttcgccc ttagcaaatg aatgattttc ttaattaaat tgattatttt
     2161 tttgaccttt tccaacatta gacttattca tatattttaa cggtggatct cataatgagg
     2221 attaactctt ccctacttta ttacaataaa aaaaggccgc gagcttggga ggggctcacg
     2281 accatactta ttattaaggg aatgttttac agttattttt tcaatctatt tttggggatg
     2341 ttattaatta tgaaaaaaaa ttttatttat caaataatga taaatcgatg ttgtttacga
     2401 tagcttacat gctagaaata attatctgta atgataatta agaaaaatac atagcactga
     2461 gtccaaggaa actaactcta ctagcaaatg ttactcactt actaatttta catggttatt
     2521 aagttgggat ggcttaataa ctcatacttt tagtactata cgaagataac aaatttacaa
     2581 tgaaagtaat taattattca tcttattttt tagtgaattt gttcactgtg tcgataatcc
     2641 attttactaa gtcaccgatt gttgaaatga tatcttgtgc cattgaaatc actccttcct
     2701 taattaagat aaaaattctt aaaattaaac aactcatcaa ctattttcca tcacatctct
     2761 gtgatctagt tatattaaaa catgctataa gcatttattt tccatttttt cttaactagt
     2821 cgttttttat tcttaactgt aaattttttt atgttaaaat attaaataca aattacattt
     2881 aacagttaag tatttatttc ctacagttag gcaatataat gataaaagat tgtactaaat
     2941 cgtataataa cagtgaggag agtggtgtaa aattgaatta ttttgataac aaaattgacc
     3001 agtttgccac gtatcttcaa aagagaaata acttagatca tattcaattt ttgcaagtac
     3061 gattagggat gcaggtctta gctaaaaata taggaaaact gattgttatg tatactcttg
     3121 cctatatttt aaacattttt atcttcactt taattactaa tatatcgttt tatctaatta
     3181 gaaggtatgc acatggtgca catgcacctt catctttttg gtgttatata gaaagcatta
     3241 cactttttat tgttctacct ctattagtac tacattttca tattaatgaa acactaatga
     3301 tgtttttagc attattaagt gttggcgtag tgattaaata tgcacctgca gctactaaga
     3361 agaagcctat cccggcaagg cttgtaaagc aaaaaagata tttttcaatt ataattagta
     3421 caattctttt cattatcaca ctttttgtaa aggaaccata tacccaattt attcaactgg
     3481 gtattattat acaagctatt acattactac caatatacta ttcaaaggag gactaaatta
     3541 tgaaaaaatt actcaacaaa gttattgagc ttttagttga ctttttcaac agcatcggtt
     3601 acagagcagc ctatataaat tgtgattttt tattggatga agctgaagta ccaaaagaat
     3661 taactcaatt acacgaataa ctataagaga aagtgtgata gtaagtggaa gcattaaatg
     3721 attataatta cgttttattt gtaattgttc aagtttcatt aatgttcttt atatctgcat
     3781 ttattagtgg aatacgatac aaaaaatcag attatatata cattattgga atagtgttat
     3841 cttcagtata tttctttgac aaaatcagaa gtatttcact agttgttata acgattttta
     3901 taataatttt tctctatttt aaaattagac tatattctgt a
//
LOCUS       DATTQF010000180         3912 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.182, whole genome shotgun sequence.
ACCESSION   DATTQF010000180 DATTQF010000000
VERSION     DATTQF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.182"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(264..1034)
                     /locus_tag="VB241_002509"
     CDS             complement(264..1034)
                     /locus_tag="VB241_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1245726.1"
                     /translation="MREKVSNRLKHIMKIRNLKQVDIINKSKPYQKQLGISLSKSTLS
                     QYINDVQSPDQDRIYLLSKTLNVGEAWLMGYDVNSYRVPDEERQEETVMSKINNISSQ
                     LTPPRQSNVLNYANSQLDEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHE
                     EVFFKEDEAPSNADFCILINGDSMEPMLKQGTYAFIKKEDYIKDGTIALVVLDGVSLI
                     KRVDICEDYINLVSLNPKYDDIKVASFSDIKVMGKVVL"
     gene            1194..1415
                     /locus_tag="VB241_002510"
     CDS             1194..1415
                     /locus_tag="VB241_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HEP1245727.1"
                     /translation="MCYDYSRLSGKIVEKYGTQYNFAIAMKLSERSLSLKLNGKVGWK
                     DSEIWKAIQLLGIPVEKIHLYFFKEKVHV"
     gene            1439..1882
                     /locus_tag="VB241_002511"
     CDS             1439..1882
                     /locus_tag="VB241_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245728.1"
                     /translation="MEQITLTKEELKEIIAKEVRNAIKGEKPISSGAIFSKVRINNDD
                     LEEINKKLNFAKDLSLGRLRKLNHPIPLKKYQHGFESIHQKAYVQDVHDHIRKLTLSI
                     FGVTLNSDLSESEYNLAAKFYRDIKNYYLYIYEKRVSELTIDDFE"
     gene            1897..2076
                     /locus_tag="VB241_002512"
     CDS             1897..2076
                     /locus_tag="VB241_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245729.1"
                     /translation="MEQTIKQFLEFRKQFTPAQWHEINRIIDGQFSKKAAELQLDDQD
                     VEVIKNIITQQKIMK"
     gene            complement(2177..2659)
                     /locus_tag="VB241_002513"
     CDS             complement(2177..2659)
                     /locus_tag="VB241_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245730.1"
                     /translation="MEFIQSTLFSNVVAFLALGLSAYSIFYTRSQNKFSFVISDLNFY
                     YENNFVELNFVVANDSSRTHTLEELIFLDKNKNVLTPINVVLESDEYSSLGIYNPSYL
                     HAPIDKQLDKPEVMIANSSSEFLYKFELEPAFIKIVSNQRINKLKKYKLISTDSYEHN
                     "
     gene            2719..3456
                     /locus_tag="VB241_002514"
     CDS             2719..3456
                     /locus_tag="VB241_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEP1245731.1"
                     /translation="MQALQTFNFEELPVNTLTIENEPYVVGNEVAKILGYSNYRNAIN
                     NHVEDEDKLRTQIRYAGQLRTVTLINESGLYSLIFDASKQSKNENIRETARKFKRWVT
                     SEVLPTLRKTGAYQVPSDPMQALRLMFEATEETKQEIKNVKDDVIDLKENQKLDAGDY
                     NFLTRTINQRVAHIQRLHAITNQKQRSELFRDINSEVKKMTGASSRTNVRQKHFDDVI
                     EMIANWFPSQATLYRIKQIEMKFENEI"
     gene            3469..3678
                     /locus_tag="VB241_002515"
     CDS             3469..3678
                     /locus_tag="VB241_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245732.1"
                     /translation="MEYIGYADANAFVKISGISKDDLEKKVYSNKEFQKECMYRFGRG
                     QKRYIKIDKAIQFIGTNLMINEYEL"
     gene            3692..3853
                     /locus_tag="VB241_002516"
     CDS             3692..3853
                     /locus_tag="VB241_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HEP1245733.1"
                     /translation="MSKTYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
BASE COUNT         1464 a          601 c          637 g         1210 t
ORIGIN      
        1 ttccgtctta ttaattgatt tggtatattc tcttaatatt ttttcgtttt catcaacaat
       61 gtcttttagt gtgtttaaaa gaaagtcaca atcacctttg gctactgcac cagcttgtga
      121 atggttgatt accactagtt aaaacttcgt atattacagt ttctcttttt attttgcaat
      181 tagttatttt cattataaac ttcctttcaa acactgctga aatagatgtc ttttatatta
      241 aagctccaca taggagctat taatcacaat acaactttgc ccattacttt aatatcacta
      301 aacgaagcaa ctttgatatc atcatacttc ggatttagag ataccaaatt aatataatct
      361 tcacatatat ctacacgttt aatcagactt actccatcta atacaacgag tgcaattgtg
      421 ccatctttaa tataatcttc tttcttaata aaagcgtatg ttccttgctt taacataggt
      481 tccattgaat cgccgttaat taaaatgcaa aaatcagcat ttgatggcgc ttcatcttct
      541 ttgaaaaata cttcttcgtg taatatatca tcgtacaatt cttctccgat accagcacca
      601 gttgcgccac acgcaatata cgacactaac ttagattctt tatattcatc tatagaagtg
      661 actttatttt gttcatctaa ctgactattc gcgtagttga gtacattgct ttgtcttgga
      721 ggcgtgagct gagatgatat gttattaatt tttgacatca cagtttcctc ttggcgttct
      781 tcatcgggta cgcgataaga atttacatca tatcccataa gccacgcttc accgacattt
      841 aaagttttag aaagtaggta aattctatct tggtcaggag attgtacatc gttaatatat
      901 tgagacaaag tacttttact taaagatata cctagttgct tttgataagg tttcgattta
      961 ttaatgatat ctacttgttt taaatttctt attttcatga tgtgttttag tctgtttgaa
     1021 actttttctc tcatttagtg cacctccgtt tgataacttt ataataaacc ttgttgaaca
     1081 aaaattcaat aaaaaagttc ataaaacatg aatttttgtg ttgacttaat tcaaaacaag
     1141 gtgtaaagta tagttaagtt cacgatacat gaacttcaaa ggaggtgttt tttatgtgtt
     1201 acgactactc gcgtttgagt ggtaagatag ttgaaaagta tggcactcag tacaatttcg
     1261 ctattgctat gaagttgtcc gagagaagct tatccttaaa actcaacgga aaagttgggt
     1321 ggaaagatag cgaaatatgg aaagctatac aattgctagg tataccggta gaaaaaatac
     1381 atttatattt ttttaaagaa aaagttcacg tttgatgaac tagaaagtgg aggacatcat
     1441 ggaacaaatc acgttaacca aagaagagtt gaaagaaatt atagcgaaag aagttagaaa
     1501 tgctataaaa ggcgagaaac caatcagttc aggtgcaatt ttcagtaaag taagaatcaa
     1561 taatgacgat ttagaagaaa tcaataaaaa actcaatttc gcaaaagatt tgtcactagg
     1621 aagattgagg aagcttaatc atccgattcc actaaaaaag tatcagcatg gcttcgaatc
     1681 aattcatcaa aaagcttatg tacaagatgt tcatgatcat attagaaaat taacattatc
     1741 gatttttgga gtgacgctta attcagattt gagtgaaagt gaatataacc tagcagcaaa
     1801 attttacaga gatatcaaaa attattattt atatatctat gaaaagagag tttcagaatt
     1861 aaccatcgat gatttcgaat aaaggaggaa caacaaatgg aacaaacaat caaacaattt
     1921 ttagaattta gaaagcaatt cacacccgca cagtggcacg aaatcaacag aattattgac
     1981 ggacaattta gtaaaaaagc cgccgagcta caactcgacg accaagatgt tgaggttatt
     2041 aaaaatatta ttactcaaca aaagattatg aagtaacaat ttgaataaaa gttattcaaa
     2101 aatcacgaaa ggaagataca aaatgatgct gaccaacaca ctactagcaa ttcacttttt
     2161 catgaattta gcgatattaa ttatgctcgt aagaatcggt agagattaac ttatactttt
     2221 taagtttgtt tattcgttga ttagaaacaa tctttataaa cgcaggttct aattcgaatt
     2281 tatataaaaa ctctgatgac gaattagcta tcataacttc tggtttatct aattgtttat
     2341 cgattggagc atgcaaataa cttggattat atataccgag agatgaatat tcatcagatt
     2401 ccaatactac gttaataggt gttaaaacat ttttgttttt atctaaaaat attaattctt
     2461 ctaaagtatg agttctagac gagtcattag cgacgacaaa atttaattct acaaaattat
     2521 tttcatagta gaaattaaga tcgctaataa caaaactgaa cttattttga gaacgagtat
     2581 aaaaaattga gtatgcagat agacctaaag ctagaaaagc tacaacgttt gaaaacaaag
     2641 tagattgtat gaattccata agaataacca ccttaaatat ttgataacaa cattatacac
     2701 gaaaggagca taaacattat gcaagcatta caaacattta attttgaaga attaccagta
     2761 aatacattaa caatcgaaaa tgaaccatac gttgtaggta acgaagtagc taagatatta
     2821 ggatattcaa attatcgaaa cgctataaat aaccatgtag aggatgagga taagctacgt
     2881 acccaaatta ggtacgcagg tcaattaagg actgtgacat taatcaacga atcaggatta
     2941 tacagtttaa tcttcgatgc ttctaaacaa agtaaaaacg aaaacattag agaaaccgct
     3001 cggaaattca aacgctgggt aacatcggaa gttttaccaa cactaagaaa aactggtgca
     3061 tatcaagtac ctagcgaccc aatgcaagca ttgagattaa tgtttgaagc tacagaagaa
     3121 acaaaacaag aaattaaaaa cgtgaaagat gatgttattg atttgaaaga aaatcaaaaa
     3181 ctggatgcgg gagactacaa cttcttaact agaacaatca atcaaagagt agctcatata
     3241 caaagactac atgcgataac aaaccaaaaa caacgtagcg aattattcag ggatattaat
     3301 tcagaagtga aaaagatgac tggtgcgagt tcaagaacga acgtaagaca aaaacatttc
     3361 gacgatgtaa ttgaaatgat tgctaattgg ttcccgtcac aagctacttt atacagaatc
     3421 aagcaaattg aaatgaaatt tgaaaacgaa atataggagg ggttgaatat ggaatacatc
     3481 ggatatgcag acgcaaatgc gtttgtaaaa ataagtggca tttcaaaaga tgatctagag
     3541 aaaaaagttt actcgaacaa agagtttcaa aaagaatgca tgtacagatt tggtcgagga
     3601 caaaagcgtt atataaaaat tgacaaagct attcaattta tcggtaccaa tttaatgatt
     3661 aatgaatacg aattatagga ggagttatca aatgagtaaa acttataaaa gctacttatt
     3721 agcagtatta tgcttcacag tcttggcaat tgtacttatg ccgtttctat acttcactac
     3781 tgcatggtcg attgcgggat tcgcaagtat tgcaactttc atattctata aagaatactt
     3841 ttatgaagaa taaaaaaact gctacttgcg ccaacaagta acagtgacaa acgattaaca
     3901 aaattaattc at
//
LOCUS       DATTQF010000181         3826 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.184, whole genome shotgun sequence.
ACCESSION   DATTQF010000181 DATTQF010000000
VERSION     DATTQF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.184"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..313
                     /locus_tag="VB241_002517"
     CDS             <1..313
                     /locus_tag="VB241_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245734.1"
                     /translation="GMDITFVPANLKGLEHTTLKEKVIPNVMETLNEGALHIAEEAHH
                     IMNNHSIMIYPIDIETLFETNKWINAYECYFKNMLGLKCELQSIDAFNFIQQLDLNNN
                     S"
     gene            318..428
                     /locus_tag="VB241_002518"
     CDS             318..428
                     /locus_tag="VB241_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245735.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            476..1777
                     /locus_tag="VB241_002519"
     CDS             476..1777
                     /locus_tag="VB241_002519"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP1245736.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            1928..3190
                     /gene="clpX"
                     /locus_tag="VB241_002520"
     CDS             1928..3190
                     /gene="clpX"
                     /locus_tag="VB241_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP1245737.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            3344..>3826
                     /locus_tag="VB241_002521"
     CDS             3344..>3826
                     /locus_tag="VB241_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP1245738.1"
                     /translation="MKVNPNNIELIISAVKEDQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     I"
BASE COUNT         1510 a          501 c          715 g         1100 t
ORIGIN      
        1 tggtatggat ataacttttg taccagctaa tttaaaaggg ctagaacata caacactgaa
       61 agaaaaagtt atacctaacg ttatggaaac attaaatgaa ggtgctttac atatcgctga
      121 agaagcacac catattatga ataatcactc tatcatgatt tatccaattg atattgaaac
      181 tttgtttgaa acgaataaat ggattaatgc atacgaatgt tatttcaaaa atatgttagg
      241 acttaaatgt gaattacaaa gtatcgatgc atttaatttt attcagcaat tagatttgaa
      301 taataatagt taaattgttg caaaataata ctcagcattt taatatagtt ttgacgaaaa
      361 aagttgattt acaaatatat aaacgttgtg atttcaatgt ttgtataggg aatagaccta
      421 tgatataata gggaagttga aaaaataaat tagttaatca tggaggtata tatacatgac
      481 agcaacttgg gaaaaaaagg aaggtaacga aggtttatta acagttactg ttcctgcaga
      541 aaaagtaaac aaagcgttag atcaagcatt taaaaaagtg gttaaacaaa ttaacgtacc
      601 tggattccgt aaaggtaaag tgccacgtcc aattttcgaa caacgctttg gtgtagaagc
      661 attatatcaa gatgctatcg acattttatt accagatgct tatggtgaag caattgacga
      721 aactgatatt aaaccagttg cacaaccaga agtaagtgtt actcaaattg aaaaaggtaa
      781 agatttcatt tttgaagcaa cagttacagt tgagccagaa gttaaattag gagactataa
      841 aggtcttgaa attgaaaaac aagaaactga attatctgat gatgagttac aagaagcaat
      901 tgaccacagc ttaggacatt tagctgaaat ggtagttaaa gaagatggtg ttgttgaaaa
      961 tggcgacaca gttaacatcg actttagtgg ttcagttgac ggagaagaat tcgaaggtgg
     1021 acaagctgaa ggttacgatt tagaaatcgg ttcaggttca ttcattcctg gtttcgaaga
     1081 gcaattagaa ggtatgaaag ttgacgaaga aaaagatgtt gtcgtaacat tcccagaaga
     1141 ataccatgct gaagaattag ctggtaaaga agcaactttc aaaacaaaag ttaacgaaat
     1201 taaatttaaa gaagttccag aattaactga tgaaattgct aatgaattag atgcagaagc
     1261 aaatacagta gacgagtaca aagaaaactt acgtaaacgt ttagctgaac aaaaagctac
     1321 agatgcagaa aatgttgaaa aagaagaagc gattactaaa gctactgata atacaacaat
     1381 cgatattcct gaagcaatgg ttaatactga attagatcgt atggtgtctg aatttgcaca
     1441 aagaattcaa caacaaggtt tagatttaca aacgtacttc caaatctcag gtcaagatga
     1501 atctcaatta agagagcaaa tgaaagacga tgcagaacaa cgtgttaaaa ctaacttaac
     1561 tttaactgca atcgctgaag ctgaaaaaat cgaagctact gatgaagata tcgataaaga
     1621 attagaaaaa atgagtaaac aatttaatat ctcagttgaa gatatcaaaa atactttagg
     1681 taatactgat atcattaaaa atgatgttcg tattcaaaaa gttatcgatt tattaagaga
     1741 taacgcaaag ttcgttgaag gaactaaaga agattaatct tcattaaata ttaaattaca
     1801 aaaatgagta gcagatgcat agcttatgta tctgctacta ttctttaagc aaaaagtttg
     1861 tatgttaata tgttgcattg taatatccaa tctagtatag tctttaacga ataggggtgt
     1921 aaaaagaatg tttaaattca atgaagatga agaaaatttg aaatgctctt tctgcggaaa
     1981 agaccaagat caagtaaaaa aacttgtagc aggaagtggt gtatatattt gtaatgagtg
     2041 tattgaatta tgctcagaaa tcgtcgaaga agaattagct caaaacactt ctgaagcgat
     2101 tacagaatta cctactccta aagaaattat ggatcattta aacgaatatg ttattggtca
     2161 agaaaaagct aaaaaatctt tagctgtggc tgtttataac cactataagc gtattcaaca
     2221 attaggacca aaagaagatg atgttgagtt acaaaaaagt aacattgcct taattgggcc
     2281 aacaggtagt ggtaaaacat tattagctca aacattagcc aagacgttga atgtaccatt
     2341 tgcaattgca gatgcgacaa gtttaactga agctggttat gtaggcgatg atgttgaaaa
     2401 tatcttgttg agattaattc aagcagctga ctttgacatt gataaagccg aaaaaggtat
     2461 tatttatgta gatgaaattg ataaaattgc acgtaaatct gaaaacacat ctataacacg
     2521 tgacgtttca ggtgaaggtg ttcaacaagc attgcttaaa atcttagaag gtacaactgc
     2581 aagtgttccg ccacaaggtg gacgcaaaca tccaaaccaa gaaatgattc aaattgatac
     2641 aacaaatatc ttatttattc ttggtggtgc ctttgatggt attgaagaag tgattaagcg
     2701 ccgtcttggt gaaaaagtta ttggtttctc aagcaatgaa gctgataaat atgacgaaca
     2761 agcattatta gcacaaattc gcccagaaga tttgcaagcc tatggtttga ttcctgaatt
     2821 tatcggacgt gtgccaattg tagctaattt agaaacatta gatgtaactg cgttgaaaaa
     2881 catcttaacg caacctaaaa atgcacttgt gaaacaatat actaaaatgc tggaattaga
     2941 tgatgtagat ttagagttca ctgaagaagc tttatcagca attagtgaaa aagcaattga
     3001 aagaaaaaca ggtgcgcgtg gtttacgttc aatcatagaa gaatcgttaa tcgatattat
     3061 gtttgatgtg ccttctaacg aaaatgtaac gaaggtagtt attacagcac aaacaattaa
     3121 tgaagaaact gaaccagaat tatacgacgc agaaggtaat ttaattaata atagtaaaac
     3181 atcagcttaa tcattgatgt gttataaaag tgaaaagctc caatcaaagt tgacgctcaa
     3241 atagtttaac tttgatcgga gcttttttat aatgtatacc gaaaggttca aatattatat
     3301 aatggtaaag ttgaaagaga atataaaaaa gtgaggatat aaaatgaaag ttaatcctaa
     3361 taatatagaa ttaatcatta gtgcagtaaa ggaagatcaa tatccagaaa cagaattgtc
     3421 tgaagttgca ctgagcggtc gatctaatgt aggtaagtct acatttatca atagtatgat
     3481 tggcagaaaa aatatggcac gtacatcaca gcaacccggc aaaacgcaaa cgttaaattt
     3541 ttataatata gatgaacaac ttatttttgt ggatgttcca gggtatggat atgctaaagt
     3601 aagtaaaaca caacgtgaaa aatttgggaa aatgattgag gaatatataa ctaagagaga
     3661 gaatttacaa ttagttattc aattagttga tttaagacat gatccaacac aagatgatat
     3721 cttaatgtac aattatttga aacattttga tattcctact ttagttatat gcactaaaga
     3781 agacaaaatt ccaaaaggca aagttcaaaa gcatattaaa aatatt
//
LOCUS       DATTQF010000182         3773 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.185, whole genome shotgun sequence.
ACCESSION   DATTQF010000182 DATTQF010000000
VERSION     DATTQF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.185"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(182..1048)
                     /locus_tag="VB241_002522"
     CDS             complement(182..1048)
                     /locus_tag="VB241_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEP1245739.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVT
                     HSMDKILQDTTD"
     gene            1266..2330
                     /locus_tag="VB241_002523"
     CDS             1266..2330
                     /locus_tag="VB241_002523"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEP1245740.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     TRYLKDHHFDNVSDIIGLDA"
     gene            2485..2760
                     /locus_tag="VB241_002524"
     CDS             2485..2760
                     /locus_tag="VB241_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245741.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLIIVILAIIRFVHKMM"
     gene            complement(2946..3668)
                     /locus_tag="VB241_002525"
     CDS             complement(2946..3668)
                     /locus_tag="VB241_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP1245742.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
BASE COUNT         1270 a          704 c          557 g         1242 t
ORIGIN      
        1 aaaagttaga aaatcacttt tattgcaact caatcttgtt ttgtattata gaaattaaca
       61 atagagatta aaattttatt ttccaacaat aaaaaagcac aaatattttt attgaggcag
      121 ctgaaggcct tctcattata aaattatctg tgcatattat tatcttaatc taacgtaata
      181 actaatctgt tgtatcttgt aaaatcttat ccatagaatg cgtaacacta tcacggtaca
      241 tctccccaaa tttcaataaa tgtaccatca ataaatataa acgataaaat tcaagtctat
      301 aggatgcacc ttttgcgagt ggataatgtt tattatacgc atcataaaat tcgctcgtaa
      361 aaccaccaaa taccgttgta ataccgatat caaattctct gtcaccatat aatggtgctg
      421 gatcaaataa cgccggacga ccatcttgta agaacatata attgccaccc cataggtcac
      481 catgtaataa agatggttta ctttgatgct tttctaattc cgccacaatt tgacgtcgca
      541 ctttgtcata tactttgata tcgttggcat cccatagccc tctatttagc aactcatctt
      601 tcaaatgatc catgcgcttg tcgacaaaaa tggtacacca atcgtcttgc caatgattat
      661 caaaagaaat atcgccacct tcataaggta atgagaagcc aaatttgcct tcttcttgtt
      721 gctgactgtg taattgagct acgagttgcc ctaattggcg ttgactccct gaagcccctt
      781 cttctaaata cgtcatcact aaatacgcat caccgttaac ctcgccactt gcaattactc
      841 taggtgccgt gatacctgca cgttcaaatt catttaaacc tgcaatttct gcagcataaa
      901 atgattcttt acgtccacgt tggacaagta agaaaaatgt atccgtatcc gtttcgactc
      961 gatatgcttc gtttacatcg ccaccactca ctggtgaaat ctctttaata tcttttaaag
     1021 gtaaatgctc taaccattgt tcattcatac aaacaccttc cattacgctt actttatact
     1081 attatattgc ctaattagga tgatttaatc tcaattttat ttgtggtact tgcgtcaatt
     1141 ttcaaaactt tttagtttta actaggaaat tatgtaggga aatgctagtc tattaaaaga
     1201 atatatttca catatattaa gttgaggata gtaagcctca aatataatga aataggagca
     1261 tactcatgta caaattaatt aaacctttct tattcaaaat cgaacccgaa aaagcacacg
     1321 gactaactat cgatgcatta aaaacgttac aaaaatttcc agttttattc ccagtcgtcg
     1381 ataaactatt tacttataag aatccaacgt tatcacaaac gatacaaggt aatacgtacg
     1441 acaatccaat tggcttagca gctggtttcg acaaatcttg cgaagtacca aaagcattgg
     1501 aacaccttgg attcggtgct ttagaattag gtggtatcac acctaaacct caaccgggta
     1561 accctcaacc acgcatgttc agattattag aagatgatgc cttgataaat cgtatgggct
     1621 tcaataatat tggtatgaac aaagcactca gtcatttgcg taaaaatgct tatcaagtac
     1681 ctgttggtat caatgttggt gtgaataaaa tgacacctta tgaagcgcgt tatcaagatt
     1741 atataaaagt cattgatacg tttaaacacg acgtttcatt tttcacagtc aatatcagtt
     1801 ctccaaatac tgaaaacctt caaaatttcc atgataaaga tgaattttca atgttatgtc
     1861 aagctttgac aacatttaaa aaacagcacg atgtaacagt gccaatttac ttaaaactaa
     1921 cgtctgatat ggatttcgat ggcttaaaag cactattacc agcgattact gagacatttg
     1981 acggtatcat cttagcaaac acaacgcgac aacgagatgg tttaacttct gctaataaag
     2041 tcgaagaagg cggtttgagt ggtcgtccat tatttgaacg taatttaaaa ttgattaagt
     2101 atgcttatca gcaaacaaat ggtgaatttt taattatagg tacaggcggc gtattcagta
     2161 ctgaagatgc aatcaaaatg atgcgtcacg gtgcgtcact tattcaaatt tattcatcac
     2221 ttgttattga aggcccaggt ttaactaaga aaatgaacaa aggcatcaca cgttacttaa
     2281 aagatcatca ttttgacaat gtcagtgata ttataggact agatgcctaa tccaacgtag
     2341 tcaaataaca ctttaaaact tgctgaatcg tcttattaaa attatgatat aactcaagaa
     2401 gatatattta tcaccatttt tcaaaataat ggcttaaata tcacatatca ttaaatatag
     2461 cgttttataa cggaggcgtt tgttatgact aaagaaacag ataataaaaa aacagtagcg
     2521 acttctgaca acaaagacac acagcaacaa aatcaacctg aagaagatga acgtcaaggt
     2581 ttgaatggtt atcgtaaaac agatcttgat ttagaaatcg agcaagagct acgtgaaatg
     2641 atgaaaacag gagaaaatga aacgaaaaat gactacaaaa agtttaaagt gttttcactt
     2701 atttcaacac ttatcattgt cattttagca attataagat ttgttcataa aatgatgtaa
     2761 ttagagtgag acattgtttt atgtctcaga atccagttat tcatgatagc tacaatattt
     2821 acgatgatat aaataacccg agattttagt atgattcatt tcactaaagt ctcgggtttc
     2881 tatttgataa tttttaatag ggcatggctt gtatatgcgc ctgcctttta tcttatttcc
     2941 aatgattaat ctggatattg ttctaaaaat gctttcgctt ctttattaac tgttttaaaa
     3001 tcaatacctt gttgcatcgc tgcaaagaca catccacaat aacactgcct aaagatatta
     3061 tagtcattac acatttctat ggaacgctca taacctttac ttttcttaaa atcacttggc
     3121 aaatagttca catcgtatat tttttggaca tccataccaa gttcattgat taattgtgcg
     3181 ttctttttag gtgataacgt gattgcactg ccaaaataat catagccatg ttctacagct
     3241 gctttcgcta caatatccaa acgcatttca aaacaagcgg tacaacgtaa gccgccttct
     3301 ttttcatcag ctaattcttt atccttcacc attttcacaa acttatgcgg ttcataaggt
     3361 gcttcaatat acttcacatt cgcaccagtc ttgcgattaa aatcttccac aaattgttct
     3421 tgcactttag cacgtcgtaa atattcattt ttcggatgaa tatttgaatt cgcgaaataa
     3481 attgcaatgt ctgcatattg tgttaaaaac tctaatgtat atgtactaca aggtgcacaa
     3541 caactatgca ataagatttt aggtctgatt gcttctcttt cccactgccc gattaatttc
     3601 ttcaacacct tgtcataatt aattttttga tttttcattt tactgataat aggttcagca
     3661 ttaatcatga tttaacctcc cacatttaat cattaacttc tattatatat gattcatatt
     3721 aaatgtcagt caaaaaagtt agaaattcat tttaatgcat ttatattttg aaa
//
LOCUS       DATTQF010000183         3758 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.186, whole genome shotgun sequence.
ACCESSION   DATTQF010000183 DATTQF010000000
VERSION     DATTQF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.186"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            155..1882
                     /locus_tag="VB241_002526"
     CDS             155..1882
                     /locus_tag="VB241_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245743.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            2241..3470
                     /locus_tag="VB241_002527"
     CDS             2241..3470
                     /locus_tag="VB241_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP1245744.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIGGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
BASE COUNT         1269 a          494 c          714 g         1281 t
ORIGIN      
        1 atagaaatat tctatcatat aaatgttatg agcggtattt tggggcaata ctttatttga
       61 tttttaaagt tttgttggga gaaaatatat gatagaaatg catgtatcta tctaaatgaa
      121 ttaactataa atttcaaaca gaagaggtaa aactatgaaa cgagaaaatc cattgttttt
      181 cttatttaaa aaactatcat ggccagtggg tcttatcgtt gcagctatca ctatttcatc
      241 actagggagc ttaagtggac tattagtgcc actgtttact ggacgaattg tagataaatt
      301 ttccgtgagt catatcaatt ggaatctaat cgcattattt ggtggtatct ttgtcatcaa
      361 tgctttatta agcggattag gtttatattt attaagtaaa attggtgaaa aaattattta
      421 tgcgatacgc tcagttttat gggagcatat catacaatta aaaatgccat tctttgacaa
      481 aaatgaaagt ggtcaattaa tgagtcgatt aactgacgat acgaaagtga taaatgaatt
      541 tatttcacaa aagctaccta acttattacc atcaatcgtt acattagttg ggtcactaat
      601 catgttattt attttagatt ggaaaatgac attattaaca tttataacga taccgatatt
      661 tgttttaatt atgattcctc taggacgtat tatgcaaaag atatcgacaa gtacacaatc
      721 tgaaattgca aactttagtg gtttgttagg gcgtgtccta actgaaatgc gtcttgttaa
      781 aatatcaaat acagagcgac ttgaattaga taatgcacat aaaaatttga atgaaatata
      841 taaattaggt ttaaaacagg ctaaaattgc ggcagttgta caaccaattt caggtattgt
      901 tatgttgcta acaattgcaa ttattttagg ttttggtgca ttagaaattg cgactggtgc
      961 aatcactgca ggtacattaa ttgcaatgat attttatgtt attcaattat cgatgccttt
     1021 aatcaatctt tcaacgttag ttacagatta taaaaaggca gtcggtgcaa gtagtagaat
     1081 atacgaaatc atgcaagaac ctattgaacc gacagaagct cttgaagatt ctgaaaatgt
     1141 attaattgat gacggtgtgt tgtcatttga acatgtagac tttaaatatg atgtgaagaa
     1201 aatattagat gatgtgtcgt tccaaatccc acaagggcaa gtgagtgctt ttgtaggtcc
     1261 ttctggatct ggtaaaagta cgatatttaa tctgatagaa cgtatgtatg aaattgagtc
     1321 aggtgatatt aaatatggcc ttgaaagtgt ctatgatatc ccgttatcta agtggcgacg
     1381 caaaattgga tatgttatgc aatcaaattc gatgatgagt ggtacaatta gagacaatat
     1441 tttatacgga attaatcgcc atgtttcaga tgaagaactt attaattatg ctaaattagc
     1501 gaactgtcat gattttatca tgcaatttga tgaaggatat gacacgcttg taggtgaacg
     1561 aggattgaaa ctgtctggcg gacaacgtca acgtattgat attgctagaa gttttgttaa
     1621 aaatcctgat attttgttac ttgatgaagc aacagctaat ctcgatagtg aaagtgaatt
     1681 gaaaattcaa gaagctttag aaacattgat ggaaggtaga acaacgattg tcattgcgca
     1741 tcgtttgtct acaattaaaa aagccggtca aattatattc ttagacaaag gacaggtaac
     1801 aggtaaaggt acgcattcag aactgatggc atcacatgcg aagtataaaa actttgtagt
     1861 gtctcaaaaa ttaacagatt aaatttatat atataagtaa gcttggagca agtacagata
     1921 taccatcgag aaaattaaaa tgtggcacat tgatggatat agatgttaat aaattgcttc
     1981 aagcttttgt ctattttaaa tcatgtgaga agttacgaca taataattct taaattaatg
     2041 aaatcgatat tttaagaaaa aaatgctcat ggtataatac aagttataag caaacataca
     2101 tatattaaat actgtagcca cgagtcataa ttcttcatat tttacatagc aatttaactg
     2161 attttagagt ccacggtaca gaagtttgat atttcaatgt ttctaaattt ttaaaaaatt
     2221 aaatcatagg tgggtgccaa atgtttttat taatcaacat tattggtcta attgtatttc
     2281 ttggtattgc ggtattattt tcaagagatc gcaaaaatat ccaatggcaa tcaattggga
     2341 tcttagttgt tttaaacctg tttttagcat ggttctttat ttattttgat tgggggcaaa
     2401 aagcagtaag aggagcagcc aatggtatcg cttgggtagt tcagtcagcg catgctggta
     2461 caggttttgc atttgcaagt ttgacaaatg ttaaaatgat ggatatggct gttgcagcct
     2521 tattcccaat tttattaata gtgccattat ttgatatctt aatgtacttt aatattttac
     2581 cgaaaattat tggaggtatt ggttggttac tagctaaagt aacaagacaa cctaaattcg
     2641 agtcattctt tgggattgaa atgatgtttt taggaaatac tgaagcatta gccgtatcaa
     2701 gtgagcaact aaaacgtatg aatgaaatgc gtgtattaac aatcgcaatg atgtcaatga
     2761 gctctgtatc gggagctatt gtaggtgcgt atgtacaaat ggtaccagga gaactggtac
     2821 taacggcaat tccactaaat atcgttaacg cgattattgt gtcatgcttg ttgaatccag
     2881 taagtgttga agagaaagaa gatattattt acagtcttaa aaacaatgag gttgaacgtc
     2941 aaccattctt ctcattcctt ggagattctg tattagcagc aggtaaatta gtattaatca
     3001 tcatcgcatt tgttattagt tttgtagcat tagctgatct atttgatcgt tttatcaatt
     3061 tgattacagg attgatagga ggatggatag gcataaaagg tagtttcggt ttaaaccaaa
     3121 ttttaggtgt gtttatgtat ccatttgcgc tattactcgg tttaccttat gatgaagcgt
     3181 ggttggtagc acaacaaatg gctaagaaaa ttgttacaaa tgaatttgtt gttatgggtg
     3241 aaatttctaa agatattgca tcttatacac cacaccatcg tgcggttatt acaacattct
     3301 taatttcatt tgcaaacttc tcaacgattg gtatgattat cggtacattg aaaggaattg
     3361 tagataaaaa gacatcagac tttgtatcta aatatgtgcc tatgatgcta ttatcaggta
     3421 tcctagtctc attattaaca gcagctttcg ttggtttatt tgcatggtaa tatgtcgaag
     3481 agtgactacg ataatacatt ttaactaaaa aagatgtcca ggcatgtcgt ctattgatat
     3541 aggtgagatg cttggacttt tttattattg atataaagat atttaaatat ttttaaagtt
     3601 atcgaaattg atgcaatata aaaaccaagt gcacatggta atacacttgg cttttatggg
     3661 aaatgaatat tattgtacat atgacagtaa ggactaggta cagtcatagt acttcgagca
     3721 aaatttgttt tgttattata aacaacacaa aggagata
//
LOCUS       DATTQF010000184         3747 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.187, whole genome shotgun sequence.
ACCESSION   DATTQF010000184 DATTQF010000000
VERSION     DATTQF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.187"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            185..688
                     /locus_tag="VB241_002528"
     CDS             185..688
                     /locus_tag="VB241_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP1245745.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGHYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            669..1205
                     /locus_tag="VB241_002529"
     CDS             669..1205
                     /locus_tag="VB241_002529"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEP1245746.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFKQIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            complement(1497..1694)
                     /locus_tag="VB241_002530"
     CDS             complement(1497..1694)
                     /locus_tag="VB241_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245747.1"
                     /translation="MNCYDEIYNTIKKLIENKEITSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(2188..3528)
                     /gene="glnA"
                     /locus_tag="VB241_002531"
     CDS             complement(2188..3528)
                     /gene="glnA"
                     /locus_tag="VB241_002531"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP1245748.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILESGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(3547..>3747)
                     /locus_tag="VB241_002532"
     CDS             complement(3547..>3747)
                     /locus_tag="VB241_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP1245749.1"
                     /translation="ERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVD
                     ATQKPIGETLPINRGDLSRFIK"
BASE COUNT         1255 a          607 c          580 g         1305 t
ORIGIN      
        1 agattctaat attgatcctg aaaagtttga gaaagataat aaaccagttt atgaagaaaa
       61 taatatgaaa aaataaagaa tcaactccct atacgcttta gcggtggtat tgggagttga
      121 tgaacatata tcctcttata acttatttta taaataacct ttttaaaagt aaaggaggat
      181 atacatgaaa aaaagaaaaa aacgtaaaga attagactat aaaacacgtc tagctatcgc
      241 aaagtattta aatgatggtt taaacatcat tcagatttct aagattttag gtcgtgatga
      301 tgcggttctt tatagagaag taaataaacg ttctgttgat ggtgtttata atcctttatt
      361 agctgaggaa gatgctataa aatcgcgtag tatttcagat gaacaagttg ctaaacgtag
      421 acgtttgaat gatgaaacaa aagcatatat tgaagaaaaa ctttcattaa aatggtctcc
      481 tagacagata gcaagtcaaa ttgaaaaaga tacagggcat tatataagtt atcctactat
      541 atatagatat attcgcaatg gattggtaaa agttgatgtt aagcgtgata tgagacaagc
      601 aggcaagaaa tataataagt cttctgaaaa gcgtggcaaa ttagacgtag ggtctcgtgt
      661 tataaagtat cggctcaaaa agtattaata atcgtgaaga atttggacat tatgaaattg
      721 atactgtttg gagtgttaga ccatcaacat attgcttgtt aactattatt gaaagaaaaa
      781 caagatattt atatgctagt agattaaata cacgtaaatc caatgtagtt tgtaatgaga
      841 ttataaatat tatgaaacca ttattaccca aaagtataac tatggataga ggtaaggagt
      901 ttgctctatt taaacaaata gaagatgaat tgaattgtag tatttatttt agtgatttgg
      961 gttgtccata tcaacgtggt tccattgaaa atgcaaatgg acttttaaga cagtattatc
     1021 ctaaaggtac ggattttgca aatataacac aaaagtcatt agatgaagct gttaaacaaa
     1081 ttaatgcacg acctcgaatg atatttgact ataaatcgag tgaggaaatg ctaaagtacc
     1141 atgtaagcac acaaaattgt gaacctatat taaatgactg tgtgaggcac gaacccgtca
     1201 attaatatag gttcatctaa gcgaatggtt tacaatgagc gcggaaggac aggacgaata
     1261 aattttgtat ataaaatgca taatctaaaa agaagcatac tatttaaatc tgtggttttt
     1321 gacagatatt actagtaaaa tgcttctttt tcttcttctg tactctttta gtaatattta
     1381 ttaaaaaaat ttaagaaatt ctcatttttc tattgacaca ctgccatact ataaaaacac
     1441 ccactcagtc actagtttgg gcagttattg tatgcctatt gaactcaatg cttatattat
     1501 aatacctttt tcgcatattc atataagatc tttgcattct ttaagcttaa attttctatt
     1561 cttctttctc cacggcgcat agcattaata ttaccgtaac ttatcccagt atctttatta
     1621 atttgataac tcgttatctc tttgttttct atcaattttt tgattgtatt gtatatttca
     1681 tcatagcaat tcataaatta gatgaggcaa aaatttttaa ttttttagaa tatcaatagt
     1741 agtataacta aaatgaaaat accgatcgat aaacaaaaag atattttttg ttttgtttct
     1801 cttttcatat agtattaccc ccttaataat gagtagtaag gcccctattt tcggggtctt
     1861 accttagaac gttctgcgaa tgaattcgat gagaagtaat atgaatgtgg ctattttcaa
     1921 gtaatactca acgttttcgc gacgttcttt tatcacctca tctcatcacc tccaaatata
     1981 ttaaaattca tgtgaactaa aatataaaat ggtcttcccc agctttaaaa aaataaatac
     2041 ataaaacatt tacttgacca aaacttgacc aaatccattt ttttacaaaa gtggtcaagt
     2101 gaaattccac ctttccaaat caaaaaagag ctaaagcaaa acgctctagc ccttgatatt
     2161 actgatttcc catcattgtt tagcgtatta atattgcttc atgtactgat ctctttccca
     2221 ttcagatact tgagttctat agtaatccca ttcaattgat tttgaattaa taaattgatt
     2281 atagatatga tttcctaaag cttttttaat aacttcattt tcacgcattg cttttaatgc
     2341 agtataaagt gttgaaggta agtcttgaat gcctactgct tcacgttctt cacggttcat
     2401 ttcgtaaata ttttggttaa ctggttctgg aacttttaat ttatttttaa tgccgtctaa
     2461 accagattct aaaattgcag ctaacgccat atatgggttc gcagctgggt caactgaacg
     2521 tacttcaata cgagttgata atcctcttga agatggtaca cggattaatg gtgaacggtt
     2581 tttaccactc catgcaatat aacatggcgc ttcataacca ggtactaaac gtttatatga
     2641 gtttactaac gggttacata cagcagtaaa tcctcgtgca tttttaagta cacctgctgt
     2701 aaattgatat gctgtttccg ttaagcccat ttcagtattt ggatcgaaga atgcattttc
     2761 tttacctttg aataatgaaa cgttaaagtg cataccgcta ccattcacac caaataatgg
     2821 tttaggcata aatgttgcgt gtaaattatg tttacgtgcg attgttttaa caaccaattt
     2881 aaatgtttgg atattatcac atgctgtaac agcatctgca tatttaaagt caatttcatg
     2941 ttgaccaggg gcaacttcat ggtgactagc ttcaatatcg aagcccatat cctctaattc
     3001 taaaacaata tcacgacgac agttttcacc taaatctgta ggtgctaaat cgaaatatcc
     3061 accatcatca ttaagttcta aagttggttc ccctttttca tccaacttaa acaagaagaa
     3121 ttctggttca ggccctaggt taaagtctgt gaagcctaaa tcttccattt cttttaatac
     3181 acgttttaag tttgcacgag gatccccttc aaatggtgtt ccatctgttt tatatacatc
     3241 acaaattaaa cgtgcaactt taccttgtcc agcagtccat gggaagatta cccaagtatc
     3301 taaatctgga tgtaagtaca tatctgattc ttcgatacgt acgaaacctt cgatagaaga
     3361 accgtcaaac atcatttcgt tatcaagtac tttttctaat tggcttacag gcacttcaac
     3421 atttttaatt gttcctaaaa tatcagtgaa ttgtaatctt aaatatctta cattttcctc
     3481 ttctgcaaat ttacgaatgt cgtctttagt gaaagtacgt tttggcattt taaaatcctc
     3541 caaattttat ttaataaatc gggataaatc accacgattt attggcaaag tttctccaat
     3601 aggcttttgc gtggcatcta caatcatctt ttttcttatc tcttgttcat ctgttgttaa
     3661 atgctcttgt gagtcataaa tgatttgttt aatccctttg atattaaatc ctttttctaa
     3721 taatgattta atttctagta atctttc
//
LOCUS       DATTQF010000185         3718 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.188, whole genome shotgun sequence.
ACCESSION   DATTQF010000185 DATTQF010000000
VERSION     DATTQF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.188"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            177..1655
                     /gene="spa"
                     /locus_tag="VB241_002533"
     CDS             177..1655
                     /gene="spa"
                     /locus_tag="VB241_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HEP1245750.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNKFNK
                     DQQSAFYEILNMPNLNEEQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDGNKPGKEDGNKPGK
                     EDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHA
                     DANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
     gene            complement(1984..3576)
                     /locus_tag="VB241_002534"
     CDS             complement(1984..3576)
                     /locus_tag="VB241_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEP1245751.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1392 a          714 c          575 g         1037 t
ORIGIN      
        1 agcgtgattt tgcggtttta agccttttac ttcctgaata aatctttgga caaaattttt
       61 attttataag ttgtaaaact tacctttaaa tttaattata aatatagatt ttagtattgc
      121 aatacataat tcgttatatt atgatgactt tacaaataca tacagggggt attaatttga
      181 aaaagaaaaa catttattca attcgtaaac taggtgtagg tattgcatct gtaactttag
      241 gtacattact tatatctggt ggcgtaacac ctgctgcaaa tgctgcgcaa cacgatgaag
      301 ctcaacaaaa tgctttttat caagtgttaa atatgcctaa cttaaacgct gatcaacgta
      361 atggttttat ccaaagcctt aaagatgatc caagccaaag tgctaacgtt ttaggtgaag
      421 ctcaaaaact taatgactct caagctccaa aagctgatgc gcaacaaaat aagttcaaca
      481 aagatcaaca aagcgccttc tatgaaatct tgaacatgcc taacttaaac gaagagcaac
      541 gcaatggttt cattcaaagt cttaaagacg atccaagcca aagcactaac gttttaggtg
      601 aagctaaaaa attaaacgaa tctcaagcac cgaaagctga caacaatttc aacaaagaac
      661 aacaaaatgc tttctatgaa atcttgaaca tgcctaactt gaacgaagaa caacgcaatg
      721 gtttcatcca aagcttaaaa gatgacccaa gtcaaagtgc taacctttta gcagaagcta
      781 aaaagttaaa tgaatctcaa gcaccgaaag ctgataacaa attcaacaaa gaacaacaaa
      841 atgctttcta tgaaatctta catttaccta acttaaatga agaacaacgc aatggtttca
      901 tccaaagctt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa gctaaaaagc
      961 taaatgatgc acaagcacca aaagctgaca acaaattcaa caaagaacaa caaaatgctt
     1021 tctatgaaat tttacattta cctaacttaa ctgaagaaca acgtaacggc ttcatccaaa
     1081 gccttaaaga cgatccttca gtgagcaaag aaattttagc agaagctaaa aagctaaacg
     1141 atgctcaagc accaaaagag gaagacaaca acaagcctgg caaagaagac aacaacaagc
     1201 ctggcaaaga agacaacaac aagcctggca aagaagacgg caacaaacct ggtaaagaag
     1261 acaacaaaaa acctggcaaa gaagatggca acaaacctgg taaagaagac ggcaacaagc
     1321 ctggtaaaga agatggcaac aagcctggta aagaagacgg caacggagta catgtcgtta
     1381 aacctggtga tacagtaaat gacattgcaa aagcaaacgg cactactgct gacaaaattg
     1441 ctgcagataa caaattagct gataaaaaca tgatcaaacc tggtcaagaa cttgttgttg
     1501 ataagaagca accagcaaac catgcagatg ctaacaaagc tcaagcatta ccagaaactg
     1561 gtgaagaaaa tccattcatc ggtacaactg tatttggtgg attatcatta gcgttaggtg
     1621 cagcgttatt agctggacgt cgtcgcgaac tataaaaaca aacaatacac aacgatagat
     1681 atcattttat ccaaaccaat tttaacttat atacgttgat taacacattc ttatttgaaa
     1741 tgataagaat catctaaatg cacgagcaac atcttttgtt gctcagtgca ttttttattt
     1801 tacttacttt tctaaacaac ttctgaaacg cctcaacact ttctactctg attacatata
     1861 cgacattttt agacattaaa aaatcgaact agacaagatg ctcattgtat ttcgtactag
     1921 ttcgattcat gaataattag atttaaaatg tcatttgaat ccaagtgaca acattattta
     1981 tatttagaat attaacgtta gtataaacgt ccaaacacaa ataaaggcaa caaatataat
     2041 actgtatttt aacgtcattt ttaataatgc agattcttca ccaacttttt taacagctgc
     2101 agtcgcaatg gcaattgatt gtggtgaaat aagtttcgct gctacaccac ctgcagtgtt
     2161 agccgccaca agtaatgaac cgcttgttga aatttgttgt gccactgtcg cttgaatagg
     2221 tgcgaataaa gtattgttat ttacaactga accagtcata aacacaccaa tccaacctaa
     2281 tactggagag aataatggga aaattgctcc cgctttagca ataccttgtc caattgctac
     2341 agtcaaacca ccgtatgtca taactttagc aatagctagg atagctgaaa ttgtaaggat
     2401 cggtaaccat aattctttaa ttgcttcgac caataaagca cctgcacttt tccattttaa
     2461 cttcgtaatt aaaattgtaa taattactgt taataaaatc gctgtcccag ttgcaccaat
     2521 taaatcgaga cgcaacgcaa ttcctttagg cgataaatca ctcacagtat ttggaattgg
     2581 caattttatt actaaacttt caagtgcacc tccaggttgg aataattttt tgaagaatgg
     2641 tgcactccat actaatacaa aggcagttaa aatgacgaac ggactccaag caaagacaat
     2701 ttctttaggc gttcgttttt gaattttatg ttcagacgct tccaatctga aaatgttttt
     2761 cggtttaaat ttacgacaaa caaatgctaa caccaccatt gttgctagtg atggaataat
     2821 gtctgctagt tctggaccat ggaatattgt taataataat tgtaatccag tatatgtacc
     2881 actcactgtt aaaatgacag gtaaaatttc tttaatacct ttcataccat ctacaatgaa
     2941 tactaaaaca aatggaataa taaagtttaa aattggaagt gttaatgctg agtatctcgc
     3001 aacatctaat gttgtaacgc ctccacttaa gttaaacgta tcaataatac taactggtaa
     3061 accaattgca ccaaaggcac ccgccgcacc attagcaatt aaacataaca tcgctgcttt
     3121 taatggttca aatccaagtt gaattaataa tactgcacaa atcgcaattg gcacaccaaa
     3181 tcctgctgca ccttctaaaa atgcgttgaa acaaaatcca attaataata gttggattct
     3241 ttggtcctct gaaatacttg caatactatc ttgaataata gaaaattgtc ctgttttaat
     3301 agaaacttta tataaccaaa ctgccattaa aacgatatat cctattggga aaataccggc
     3361 aacaacgcct tctgtaatcg cacctgctga tacacgcgct ggtaattcaa atacaaataa
     3421 agccacaatc aatgtaacaa ccaaagttgt caatgctgca taaatgcctt tcattttaaa
     3481 aacggttaag cataataaaa ataaaataat aggtactgct gcaactaagg ctgataatcc
     3541 aacattatcg aatggattta cagtaagtag tgtcataatg actccctctc tttatataaa
     3601 atatttatca tcctgattaa tctacaacct atttcaactt atattttgcg atgatcacat
     3661 atttaaaatg taacactcct atatgtgaca ggcaatcgaa tttttacaaa aagttcac
//
LOCUS       DATTQF010000186         3718 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.189, whole genome shotgun sequence.
ACCESSION   DATTQF010000186 DATTQF010000000
VERSION     DATTQF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.189"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            69..1952
                     /locus_tag="VB241_002535"
     CDS             69..1952
                     /locus_tag="VB241_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP1245752.1"
                     /translation="MGMEAYKIEHLNKSYADKIIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAERELTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQHSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            1964..>3718
                     /gene="recQ"
                     /locus_tag="VB241_002536"
     CDS             1964..>3718
                     /gene="recQ"
                     /locus_tag="VB241_002536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEP1245753.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLET"
BASE COUNT         1418 a          492 c          714 g         1094 t
ORIGIN      
        1 gatttcaaaa aggtgtataa tgtatagaac tgatttaagt acgtagagat ttcaacagaa
       61 gaaggaagat gggtatggaa gcatataaaa ttgaacattt aaataaatct tatgccgata
      121 agattatatt cgataatcta gatttatcaa tttcagaagg tgaaaaaata ggtttagtag
      181 gcataaatgg tacagggaaa agtacgttgt taaaagtaat tggtggtatt gatgatgatt
      241 ttacagccaa tgttatgcat ccaaatcaat atcgaattcg ttattcgtct cagaaacagg
      301 atcttaatga agatatgact gtttttgatg cagtattaag ttctgataca acaactttac
      361 gcatcatcaa gcaatatgaa caggcagtac aagcttatgc ggatgatcaa agtgataaat
      421 tgttcaagcg aatgatggat gcgcaagatg ctatggatca acatgatgct tgggactata
      481 acgctgaaat taaaacgatc ctctcaaaac taggtataca tgatactact aaatacatta
      541 aagaattatc cggcggacaa caaaaacgtg ttgtacttgc taaaacatta atagaacaac
      601 cagatttatt gttattagat gaacctacga accatttaga cttcgaatca atcagctggt
      661 tgatcaatta tgtgaagcaa tatcctcata cagttttatt cgtaacccat gatcgatatt
      721 ttttaaatga agtttccact agaattattg aactaaacag aggtaagtta gcgtcatatc
      781 ctggtaacta tgaatcttat attgaaatgc gcgctgaaag agaactaaca cttcaaaagc
      841 aacaacaaaa gcaacgagct ttatataagg aagaacttgc ttggatgagg gctggagcta
      901 aggcacgtac tacaaagcaa caagctagaa ttaatcgatt taatgaccta gaaaatgaag
      961 ttaaccagca atataaagac gataaaggtg aattgaatct tgcttattca agattaggta
     1021 agcaagtgtt cgaattagaa gacttatcaa aggctattaa tgataaagta ttatttgaac
     1081 atctgacgga aattattcaa aaaggtgagc gtattggtgt tgttgggcca aatggagctg
     1141 gtaaaacaac actcttaaat attttgagtg gagaagacca acaattcgaa ggtaaattga
     1201 agactgggca gacggttaaa gtagcttatt ttaagcaaac agatgagacc ctggatagag
     1261 atattcgtat gattgattat ttaagagaag aaagtgagat tgcaaaagaa aaagatggaa
     1321 cctcggtatc tattacacaa cttcttgaac gatttttatt tccaagtgca actcatggta
     1381 aaaaagttta taaattatct ggtggagagc aaaagcgttt gtatttatta cgtctactcg
     1441 tacaccagcc aaatgttctg ttgttagatg aaccgacaaa tgatttagat actgagactt
     1501 taacaatact tgaagattat attcatactt tcggtggtac agtgattacc gtaagccatg
     1561 atcgctactt cttaaataaa gttgcacagt catattggtt tattcatgat ggtcagatgg
     1621 aaaagattat cggaactttt gaagattatg aaagttataa aaaatcatta gataaaaata
     1681 aatccacatt gaagcaacac tctaaatctt ctacaactgt acgtaagaaa aatggtttat
     1741 catataaaga aaaattagaa tatgaacaat tgatgaaacg catagaacaa gcggaagtaa
     1801 gaatggaaga aattgatgtg ctcatgattg aggcaagtgc agattatggg aaaattaaag
     1861 aattaaacga agaaaaagaa caacttgaaa ttcaatatga tttagacatc acaagatgga
     1921 gtgagttaga agaaattaaa gaacaacaat aaggggtcaa tttatgatgc aacaaacatt
     1981 atcgcattac tttgggtatg aaacgtttcg accaggacaa gaagaaatta ttagcaaagt
     2041 attagaccat cgtaatgtgc ttggtgtctt accaactggt ggaggtaagt ctatatgcta
     2101 tcaagtacca ggtttattgt taggtggtac aacaattgta ataagtccac taatatcatt
     2161 aatgaaagat caagtggatc aattaaaagc gatgggaatt caagctgctt ttttaaatag
     2221 tagtttgact caaaaagagc aacaacgtat tgaaaaagca ttatcaaatg gagaaattca
     2281 atttttgtat gttgcaccag aacgatttga aaaccgatat tttttaaata tgcttcagcg
     2341 tataaagatt catttagtcg cgtttgatga agcgcattgt atatctaaat ggggacatga
     2401 ttttagaccg agttatcaaa acgttatttc taaagtattt acgttacctc aagattttac
     2461 aataatagcg ttgacagcaa ctgccacggt tgaagtacag caagatatta gagaaaagtt
     2521 aaatatcgct caaactgatc aaattaaaac gagtactaag cgtagaaact taatttttaa
     2581 agtaaatcct acttatcaac gtcaaaaatt tatattggat tatattaaaa cacacgatga
     2641 agatgcaggc attatttatt gttctacacg taagcaagtt gaagagcttc aagaagcctt
     2701 agaaagccag aaaattgaaa gtgttatata tcatgcaggt ttgagcaata aagaaagaga
     2761 agaagcgcag aatgatttct tatttgatcg tgttaaagta gtcgttgcta caaatgcttt
     2821 tggcatgggt attgataaat ccaatgtacg ttttgttatt cattataata tgcctggaga
     2881 tttagaatct tattatcaag aagcgggtcg tgcaggtcgt gatgggttga aaagtgaatg
     2941 tattttgtta tttagtgaac gcgatatcaa tttacacgag tattttataa cagtctctca
     3001 agctgatgat gactataaag ataaaatggg cgaaaagtta actaaaatga ttcaatatac
     3061 aaaaacaaaa aaatgtttag aagcaacaat tgtccattat tttgaaccga atgaaaaatt
     3121 agaagaatgt gaacaatgta gtaattgtgt tcaacaagat aaatcatata atatgacaca
     3181 agaagctaag atgattatta gttgcatcgc tcgtatgaaa caacaagaga gttatagtgt
     3241 tatcattcaa gtgctaagag gagagtcaac agattatatt aagtataaag gttatgatca
     3301 aatttcaacc catggtttaa tgaaaggcta cacaacatca gaattaagtc acttaataga
     3361 tgaattaaga ttcaaagggt tcttaaatga aaatgatgaa atattaatgt gtgatacttc
     3421 aattaaaaaa ttactcagta atgaagtaga agtattcaca acaccattta agcaaaaagc
     3481 gactgaaaaa gtatttataa atacggttga aggggttgac cgagtattat tcagtcaatt
     3541 ggtagaagtt cgtaaaaagt taagtgacaa attaacgata gcacctgtaa gtatattttc
     3601 tgattacacg ttggaggaat ttgctaaacg taagcctgct tcgaaacaag atatgattaa
     3661 tattgatggc gtaggtagtt ataaattaaa acattattgt ccagcatttt tagaaacg
//
LOCUS       DATTQF010000187         3654 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.190, whole genome shotgun sequence.
ACCESSION   DATTQF010000187 DATTQF010000000
VERSION     DATTQF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.190"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(608..2758)
                     /locus_tag="VB241_002537"
     CDS             complement(608..2758)
                     /locus_tag="VB241_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP1245754.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQS"
     gene            complement(2755..3432)
                     /gene="recU"
                     /locus_tag="VB241_002538"
     CDS             complement(2755..3432)
                     /gene="recU"
                     /locus_tag="VB241_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP1245755.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEIFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1140 a          620 c          575 g         1319 t
ORIGIN      
        1 tgaattgatt aaaatgaatg ctagttatca aacaaggtta aagaataagc aggtatagac
       61 aagtttgatc atttcctaat gttgaaaaga tgtaaaacta attcatgcac acatatttgt
      121 atttatgtta ggtgtcatgt cattacaacg tcttaatata aaaaatgtcc atctacatca
      181 taatggatat cttgaggtat ggtttaagat atctatttcg aatcgtaggt ggatttctta
      241 tatctatgat tgtgaatata cgttgcaaac atgttgatat ttcactgatt aacgtactca
      301 gggaattgat aaacatatca atttaaaaca acgtctaaga tgactatgag aaaatataat
      361 cataatttta aaaatgggta caaccacttt tgcaatttca ataattttac atagctaaag
      421 atgtgaatat gaaagactat ttagatgttt caaaatgtat gtgaataact gattttacgg
      481 tttctactaa tatagcaata gcgtatgaat acatataaaa aaacctcagc tacaccataa
      541 atatggtata actgagtttt ttatgttgag tggattagtt gaatatacct gttaatccac
      601 cgctgtttca agattgttga gatctagtat tgttatttga ttgtgcagta ccactgctgt
      661 ttgctgatgt gtcactacca ccgtgtgtac tacgatttgt agtgttgtta tcttgactac
      721 cagaaacatt gattgatggg atactaccac ttactgagct aggacgttta aagtcttcgc
      781 catctctaga tgaaattttt gacatcacat tttcatataa gaactgtgga tattcttgtt
      841 ggctatgtcc cacaaatgag ttttcaccat attgtttaac tttactgaag cccatccaca
      901 ctgacatagt gtattgaggt gtaaagccgt taatccacac gtctttcgct gcattatcag
      961 gtaaattata ttgtgaataa gtttcagcac cgtaagtacc agtacctgtc ttagcaccca
     1021 tatttactcc agatacacca tggccatatg cagaaccata tggtttaaat gtacctttta
     1081 gcatctcagc taacatgtat gcagtgtaat cactcatcgc tttatggcta gtatgatcgt
     1141 attcgattgt ttcaccatca cgagtaacta ctttttgaat tgaatgcgcg ttgttataag
     1201 taccaccgtt agcgattgca gcaaatgctg atgctaattg tgttggtgag aattctgaag
     1261 cagaaccacc aagtacttca gatggaccaa tatcgccttc gtagtttaag ccaagtttgg
     1321 cagcgaattt cttaggtgca tcattaccag cattttgctt aactgattgc caagctttta
     1381 aagctgggat attgaaactt tgtcgtaaag catcataaat agatacagta ccgtgactct
     1441 tcgtatcata gtttctaaat gtagatccat caacttgata tgaagattca tcttgaatcg
     1501 catggtttgt tgcccatttc atattttcaa tggcaggtcc atacgctaag aaaggtttta
     1561 aagatgaacc agtagggtgt ggatctgttg cttggtttct gttaacgacg tctttgaaat
     1621 cacgtccgcc agatatagca actaaaccac cagttttact atcaagaatc gttgcaccaa
     1681 cttgttggtc tttattcttg tagaagctac cattatcaac atcattttgt aatgtttttt
     1741 gaacatcttt atccatgttt gtataaattt taataccact ttgtaataca ttacctaaat
     1801 tttcatcttt gaatgcttta ttattcatta attcagattt tacaaagtta acgtatgaat
     1861 tatactcaga atcttgattt gtatcaatgt tttgacgttc ttcagcagta cgatttacta
     1921 agttcgcttt taaatcgatt ttcttagcat cttcccactg tttatctgta atgcgtttat
     1981 gataatgcat taagtataaa acagtgtttt tacgatcttc agcagctttt ggatgatcat
     2041 aaatattata gttgtttgga acctgaggta aaccggctaa ataagcttct tccgctaagt
     2101 ttaaatcttt taaatcttta ttaaagtaat acttagcagc agctttaata cctgttacgc
     2161 catcagaata gtaaatttta tttagatata cttggaagat atcatcttta ctatactctt
     2221 gttctaaacg atatgataag tatgcttctt gagctttacg tccaatagat ttatgttgtg
     2281 ataaaaatgc atctttaaca acttgttgtg ttaatgttga ggcaccttca gaaccaaatc
     2341 caccagtcaa gttcttacca attgcaccga ataaacgttt ataatcaagt gcgccatgtt
     2401 cgtagaaacg attgtcttca gttgcaagta ctgcgtcttt cattgatttc ggcacgtctt
     2461 ttaaatttac atgctcatgt ctttggccat tatctaatgt tttaacaagt tctccgttct
     2521 tgtcatatat ctttgcagga atcggatctt gtaatttagc ttcggtaaaa gcaggtgctt
     2581 tccaagcata ataagcaaac aataagatac ctagtaaaag aacaacaaaa aatgcaataa
     2641 tcatgaagcc aataatctta ataatcgttc tcttcacatt tctattcttt tttgagttgg
     2701 atttcccacc attattacca tttttcttag gctgagaaga tcctttgttt tccgtcatac
     2761 gcggtcctca ctttcatcta atatcaactt atcaactgct tttagatagt ctaatcttgg
     2821 ttgatactga taaggaatat ggtaaccatt ttttcgtatt tcatcaactg ttatagactt
     2881 tttaatatta tctttatatc tcttccaaaa tatttcgaat tttgaatagg gtaaaagata
     2941 aacttcatct agcgttttaa aacgaatcat taaaaacaca ataccttttt gttgatatgc
     3001 atttttcata tgttcgactt gatggtcatg aatattattt aaaggaaagg acgtcttgtt
     3061 tttagtttcc tttgcttcaa aatcaatata ataaccttga taaacgccgt tgtaatcagt
     3121 tgttgaaggt gtacgaaaat aagcttcgtt aatcacagct ttactccgct taggatagtt
     3181 gacattaact atttgtactg gcgtaggctt tttgtgaata actgcaatgt cgcttttaag
     3241 ataaaacgca tttgaatgtt cgatatcttt ttcaagtgac ataccacgtc caccatactc
     3301 aatattacta aaggcagcgg tctttttccc tccgtctata gcactatttt tacgatatgg
     3361 tttaccattt ggataattca taaatttcac cacacaagtt tggtagtaaa cgttgtttat
     3421 tatacacaac attatagtga aataccaaac ttaaattagt ataaatgcga aagacacaat
     3481 acaccaaagc ttattttaac gtgatttcag cattaacaca ataatattat aatacattta
     3541 ccgttaatgt tggaatttta cacacaattt taattaattg aaaagttttg cttataaaaa
     3601 tgaatactca ttacaatatt tttgaatttt gttataaaag tcaaatcatg atca
//
LOCUS       DATTQF010000188         3582 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.191, whole genome shotgun sequence.
ACCESSION   DATTQF010000188 DATTQF010000000
VERSION     DATTQF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.191"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            133..1362
                     /locus_tag="VB241_002539"
     CDS             133..1362
                     /locus_tag="VB241_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEP1245756.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1557..2414
                     /locus_tag="VB241_002540"
     CDS             1557..2414
                     /locus_tag="VB241_002540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP1245757.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2414..3358
                     /locus_tag="VB241_002541"
     CDS             2414..3358
                     /locus_tag="VB241_002541"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEP1245758.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
BASE COUNT         1285 a          482 c          705 g         1110 t
ORIGIN      
        1 ctatttgtgt tacataatta aaactataag ttcttactca ttcagatata cccaatattt
       61 ttacttttat aatgtaatgc gttttatcca agttattttt taaaaataaa tattgaatta
      121 ggggctgatt tcatgtcatt aagagatgaa gcattggaaa tgcacaaacg taatcaaggt
      181 aaattagaag ttaaaccaaa tgtaaaagtt actaataaag aagaattaag tttagcatac
      241 tcacctggcg ttgctgaacc gtgtaaagat atatatgaag acaaaagaaa agtatatgat
      301 tacacaatta aaggaaatac agttgcagtt attactgatg gaacagcggt attaggttta
      361 ggtaacattg gacctgaagc aagtattcct gtaatggaag gtaaagcagt attattcaaa
      421 agcttcgctg gtatcaatgg ggtgcctatt gcgttaaata caactgatac cgaagaaatc
      481 attaaaacag ttaaattgtt agaacctaat tatggtggta ttaatttaga ggatatttcg
      541 gcaccacgtt gttttgaaat tgaagaacga ttgaaaaaag aaactaatat tccggtattc
      601 catgacgatc aacatggtac agcaattgta acaatggcag gtttggtaaa tgcattgaga
      661 gttgttaaca aagatattgc taaaataaaa gttgtactaa atggtgctgg tgcagcagga
      721 atagccattg ttaaattact atacgcgtat ggtgtaagaa atatggttat gtgtgactca
      781 agaggcgcaa tttttgaagg acgttcatat ggtatgaatc ctacgaaaga tgttgtagct
      841 aagtggacaa ataaagacaa gattgaaggg tctctagaag aagtcgtaaa agacgcagat
      901 gtatttattg gagtttctgt agctaatgcg ctgtcacaag atatggttaa gagtatggca
      961 gataatccaa ttatatttgc aatggctaat ccaaatcctg aaataatacc tgatgatgcc
     1021 aaagcagcag gtgcacgcgt tgttggtaca ggacgttcag actatcctaa ccaaattaat
     1081 aatgtattag ctttccctgg tatttttaga ggtgcattag aggttgaagc tacacatata
     1141 aatgaagaaa tgaaaaaagc agctgtagaa gcgattgctg atttaattga tagttctgaa
     1201 ttaaatgaag actactgtat cccaggaccg tttgataaac gtgtagcgcc atcagttgct
     1261 cgtaatgttg ctaaagcggc aatggaatct ggagtagcta ggattgaagt tgatccgcaa
     1321 gatgtgtatg ataaaacaat gaaacttaca gatttacaat aaaaataatt aacgtttaat
     1381 taaagtgctt aaaagttagc aataaagagt cattaaaatg atgaaaatga aagtttatga
     1441 taaacattca acagtcaaac gaatataaat caaataaatt taaacccgtt tttaactggt
     1501 caagttcagt ttaaggctct aaatggttag aacagaggtt atttggaggt tttcctatgt
     1561 ttaaagattt ttttaatcga acaaagaaaa agaaatatct tacagtacaa gactctaaaa
     1621 ataatgatgt gcctgcaggt attatgacta agtgtccaaa gtgtaagaaa attatgtaca
     1681 caaaagaatt agctgaaaat ttaaatgtgt gctttaattg tgatcatcat attgctttaa
     1741 ctgcgtataa acgtatagaa gcaatttctg atgaaggatc atttacagaa ttcgataagg
     1801 gaatgacctc tgcgaatcca ttagattttc caagttattt agaaaaaatt gaaaaggacc
     1861 aacaaaagac aggtcttaaa gaagcagtag tgactggtac agcgcaacta gatggtatga
     1921 aatttggcgt tgctgtcatg gattcacgtt ttagaatggg aagtatggga tcggttattg
     1981 gtgaaaagat atgtcgcatc attgattact gcactgagaa ccgtttacca tttatacttt
     2041 tctctgcaag tggtggtgca cgtatgcaag aaggtattat ttccttgatg caaatgggta
     2101 aaaccagtgt atctttaaaa cgtcattctg acgctggact attatatata tcatatttaa
     2161 cacatccaac tactggtggt gtatctgcaa gttttgcatc agttggtgat ataaatctaa
     2221 gtgagccaaa agcattgata ggttttgcag gtcgtcgagt tattgaacag acaataaacg
     2281 aaaaattgcc agatgatttc caaactgcag aatttttatt agagcatgga caattggata
     2341 aagttgtaca tcgtaatgat atgcgtcaaa cattgtctga aattctaaaa atccatcaag
     2401 aggtgactaa ataatgttag attttgaaaa accacttttt gaaattcgaa ataaaattga
     2461 atctttaaaa gaatctcaag ataaaaatga tgtggattta caagaagaaa ttgacatgct
     2521 tgaagcgtca ttggaacgag aaactaaaaa aatatataca aatctaaaac catgggatcg
     2581 tgtgcaaatt gcgcgtttgc aagaaagacc tacgacacta gattatattc catatatctt
     2641 tgattcgttt atggaactac atggtgatcg taattttaga gatgatccag caatgattgg
     2701 tggtattggc tttttaaatg gtcgtgctgt tacggttatt ggacaacaac gtggaaaaga
     2761 tacaaaagat aatatttatc gaaattttgg tatggctcat ccagaaggtt atcgaaaagc
     2821 actacgttta atgaaacaag ctgaaaaatt caatcgtcct atctttacat ttatagatac
     2881 aaaaggtgca tatcctggta aagctgctga agaacgtgga caaagtgaat ctattgcaac
     2941 aaatttgatt gagatggctt cactaaaagt accagttatt gcgattgtca ttggtgaagg
     3001 tggcagtgga ggtgccctag gtattggtat tgccaataaa gtattgatgt tagagaatag
     3061 tacctactct gttatatctc ctgaaggtgc agcggcatta ttatggaaag acagtaattt
     3121 ggctaaaatt gcagctgaaa caatgaaaat tactgcccat gatattaagc aattaggtat
     3181 tatagatgat gtcatttctg aaccacttgg cggtgcacat aaagatattg aacaacaagc
     3241 tttagctatt aaatcagcgt ttgttgaaca attagattca cttgagtcat tatcacgtga
     3301 tgaaattgct aatgatcggt ttgaaaaatt cagaaatatc ggttcttata tagaataatc
     3361 aacttaagca tttttatgtt aaatcgttac tgggttttac cataaattga agtacattaa
     3421 aacaataatt caatatttag atactgaatt ttaactaaga ttagtagtca aaattgtggc
     3481 tactaatctt tttttaatta agttaaaata aaattcaata tttaaaacgt ttacatcaat
     3541 tcaatacatt agttttgatg gaatgacata tcaatttgtg gt
//
LOCUS       DATTQF010000189         3548 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.192, whole genome shotgun sequence.
ACCESSION   DATTQF010000189 DATTQF010000000
VERSION     DATTQF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.192"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            255..2960
                     /gene="acnA"
                     /locus_tag="VB241_002542"
     CDS             255..2960
                     /gene="acnA"
                     /locus_tag="VB241_002542"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEP1245759.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSKLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRGAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGSVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3141..>3548
                     /locus_tag="VB241_002543"
     CDS             3141..>3548
                     /locus_tag="VB241_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEP1245760.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKV"
BASE COUNT         1199 a          523 c          696 g         1130 t
ORIGIN      
        1 tttctttctg ctcaatgaaa taatacgttt aaaaattgaa ttgaatcatt cacgaaattg
       61 ataattattc tcaattaata aaaaatactt attacacctt acctgcatga taagttttac
      121 ttatatactc tgattaaaaa gtcaaaacct aaattaagcg ttttctaagc atgatgagtt
      181 gtttatcaat aaatataata atacaattta atcaaggcat ataaatataa aaatgtatca
      241 agggggatca ttaaatggct gcaaatttta aagagcaatc aaaaaaacat tttgacttga
      301 atggccaaag ttatacttac tatgatttaa aagctgtaga agagcaaggt attactaaag
      361 tttcgaaatt accttattca attcgtgttt tgttagaatc tttacttcgt caagaagatg
      421 attttgtaat tacagacgat catattaaag ctttaagtca gtttggaaaa gatggaaatg
      481 aaggcgaggt accatttaaa ccttctcgtg ttattttaca agatttcaca ggtgtaccag
      541 ccgtagttga tttagcttct ttacgtaaag caatggatga cgttggggga gatattacta
      601 aaattaatcc agaagtaccg gtggatttag ttattgacca ctcagttcaa gtggatagct
      661 atgcaaaccc agaagcactt gaacgtaata tgaaattaga atttgaacgt aactatgaac
      721 gttatcagtt tttaaattgg gcaacgaaag catttgataa ttacaatgca gttcctcctg
      781 caactggaat agttcaccaa gttaacctag aatatttagc aagtgttgta catgttcgtg
      841 atgtagatgg tgaaaaaact gcatttccag atacattagt tggtactgat tcacatacaa
      901 caatgataaa tggtattggc gtactaggat ggggtgttgg tggtattgaa gctgaagctg
      961 gaatgcttgg acaaccttct tatttcccaa ttccagaggt tattggtgta cgactagtaa
     1021 attcattacc acaaggcgca acagcaactg atttagcgtt gagagtaact caagagctac
     1081 gtaaaaaagg tgttgttggc aaatttgtag agttctttgg tccaggtgta caacatttac
     1141 cactagcaga ccgtgctaca atcgcaaaca tggcacctga gtatggagca acttgcggat
     1201 tcttcccagt tgatgatgaa tctcttaaat atatgaagtt aactggtaga tcagacgaac
     1261 atatcgcgct tgtaaaagaa tatttgaaac aaaaccatat gttctttgat gttgaaaaag
     1321 aagatcctaa ttatacagat gttatcgaat tggatttatc aacagttgaa gcatcgcttt
     1381 caggaccaaa acgtcctcaa gatttaattt tcttaagtga tatgaaatca tcatttgaaa
     1441 attctgtaac agctccagcg ggcaaccaag gacacggttt agataaaagt gaatttgata
     1501 agaaagcaga aattaacttc aaagatggat caaaagctac aatgaaaaca ggtgatattg
     1561 caatagcagc aattacatca tgtacaaata catctaaccc ttatgtaatg ttaggtgcag
     1621 gtttagttgc taaaaaagca gttgaaaaag gcttgaaagt tcctgaatac gttaaaactt
     1681 ctctagcacc aggatcaaaa gttgttaccg gatatttaag aggtgctggc ttacaaccct
     1741 atttagatga tttaggcttc aacttggttg gttatggatg tacaacttgt attggtaatt
     1801 caggtccttt attaccagaa attgaaaaag cgattgctga tgaggaccta ttagtgacat
     1861 ctgtattatc tggtaaccgt aactttgaag gtcgtatcca tcctcttgtt aaagccaatt
     1921 acctagcttc accacagtta gttgttgctt atgcattagc tggatcggtt gatattgatt
     1981 tacaaaatga acctattggt aaaggtaatg acggtgaaga tgtatatttg aaagatattt
     2041 ggccatcaat taaagaagtt tcagataccg ttgatagtgt tgtaacacct gaattattta
     2101 ttgaagaata taataacgta tacaataaca acgaattatg gaatgagatt gatgtaactg
     2161 atcaacctct atatgacttt gatcctaatt caacatacat tcaaaatcca tcattcttcc
     2221 aaggattatc taaagaaccg ggtacgattg ttccgttaaa tggtttacgt gttatgggta
     2281 aattcggtga ttctgtgaca actgaccaca tctctccagc aggtgcaatt ggtaaagata
     2341 cgccagctgg taaatattta caagatcacc aagtgcctat tcgtgaattt aattcatatg
     2401 gttcaagacg tggtaatcac gaagtaatgg ttcgaggtac gtttgctaat atacgtatta
     2461 aaaaccaatt agcgccaggt actgaaggtg gttttacaac ttattggcca acaaatgaag
     2521 taatgcctat ctttgatgct gcaatgaaat ataaagaaga tggtacaggt ttagttgtat
     2581 tagctggtaa cgattatggt atgggttcat ctcgtgactg ggcagcaaaa ggtacaaact
     2641 tattaggtgt taaaacggtt attgcacaaa gttatgaacg tatccatcgt tcaaatttag
     2701 ttatgatggg tgtattacca ttagagttta aaaaaggtga atcagctgat tctcttggtc
     2761 tagatggtac agaagaaatt tctgttaata ttgatgaaaa tgttcaacca catgactacg
     2821 tcaaagttac tgctaagaag caagatggtg atttggtaga atttgacgct atggttcgtt
     2881 ttgactcact tgttgaaatg gattactatc gccacggtgg tattttacaa atggttttaa
     2941 gaaataaatt agcgcaataa aaaataggta tcacagtaaa ttttttatcg gtatttgagg
     3001 cactttgata gtgctctaaa aatagctcta taaactttat gatttaatag gtcggttcaa
     3061 aacctttgtg ttcttggccg gcctcttctt gtattttatg ttagaatgag atataaagtt
     3121 aaactgaaag aaggcttaaa atgatatata gtattacaga aatagaagcg cgttatgctg
     3181 aaactgataa gatgggtgta atttatcacg ggaattatgc aacttggttt gaagttgcgc
     3241 ggttggatta tatatcgaag ttaggtttta gttatgctga tatggaaaaa caaggaatca
     3301 tttcacctgt gactgacctc aatgtcaatt ataaaaagtc tattttttat ccagaaaaag
     3361 ttaaagttaa aacttgggtt gaaaaatatt cgagattacg ttcagtgtat aaatatgaga
     3421 tttttaatga aaaaggtgaa cttgcaacta caggttccac agaattgatt tgcattaaag
     3481 aagatacttt taagcctata cggttggatc gttatttccc agattggcat gaagcctata
     3541 gtaaagtg
//
LOCUS       DATTQF010000190         3546 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.193, whole genome shotgun sequence.
ACCESSION   DATTQF010000190 DATTQF010000000
VERSION     DATTQF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.193"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..459
                     /locus_tag="VB241_002544"
     CDS             <1..459
                     /locus_tag="VB241_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245761.1"
                     /translation="SHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIF
                     NSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLI
                     AGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            465..1274
                     /locus_tag="VB241_002545"
     CDS             465..1274
                     /locus_tag="VB241_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245762.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            1274..2347
                     /locus_tag="VB241_002546"
     CDS             1274..2347
                     /locus_tag="VB241_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP1245763.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLEQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            2421..3440
                     /locus_tag="VB241_002547"
     CDS             2421..3440
                     /locus_tag="VB241_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEP1245764.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQELERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
BASE COUNT         1263 a          468 c          621 g         1194 t
ORIGIN      
        1 agtcacgatg gtgtgattaa tcaatttttc cacttattta atttaccatc attcaatttg
       61 ttatttacaa ctggtgcctt tttagtagtg gcaagttata tttatatacc atttatgatt
      121 ttacctatat ttaatagcat gaaagcaatt cctaataatt tattgcaagc ctcaagtgat
      181 ttaggtgcta gtccattcta tacttttaga aaagtaatca tgccgttaac aaaagaaggt
      241 gttatgactg ggattcaagt aacctttatt ccatcacttt cattatttat gattacaaga
      301 ttaattgcag gtaataaagt cataaatata ggtacggcaa tagaggagca atttttaaca
      361 attcaaaatt atggtatggg atcaactata gctatattct taattgtatt tatggcattc
      421 attttaatca ttacaaaatc atctaatggg agagggtgaa tcatatgaaa tggtatggaa
      481 agctgtatat cgggatactt ttagcgattt tatacatccc aatattcttt ttaatgttct
      541 attcattcaa ttcggctggt aatatgattc actttgaaca ttttacatta gagcattatc
      601 aatcattatt tcaaaatgat catttaatgt cggtcatttt taacacgata gctgtagcac
      661 ttttagcagc ctcaatttct acagttattg gtacatttgg tgccattgct atttattatt
      721 taagaaataa aaagtttaaa gtaactttac taacattgaa taatgtcttg atggtatcat
      781 ccgacgttgt cataggtgca tcattcttaa ttatgtttac aacgattggc cattttactg
      841 gtcttggttt aggattttgg acagttctaa tatctcatat tgcattttgc atacctatag
      901 ttgtgattat cgtcttacca caactgtatg aaatgaataa taatatgtta aatgctgcaa
      961 gagatttagg agcgactgaa ccacaattat taagcaacat tattattcct aatattttac
     1021 cttctattat aggaggattc tttatggctt taacttattc actagacgat ttcacagtaa
     1081 gtttcttcgt tactggtaat ggctttagtg tgttatcagt tgaagtttat gctatggcga
     1141 gaaaaggaat tagtatggaa attaatgcga tttcaacatt attgtttgct gttattgtat
     1201 taggaatact aggatattat ttgattcaat acgtgataaa taagaaaaaa ctaatcaagc
     1261 gaggtgtaaa ataatgaaac gttttttaca actcattata ggtgcattag ttgtgggtat
     1321 gctttgtctt actttaagtc attggtttaa atctaaagaa caagtgcata caaatcaaaa
     1381 aatttacgta tacaattggg gcgaatatat tgatccagag ttaattaaga aatttgaaaa
     1441 agaaactggc attcaagtcg tttatgaaac tttcgattca aatgaagcga tggaagccaa
     1501 aattcgcaat ggtggtacac attatgatgt tgcttttcct agtgaatata cagttcaaaa
     1561 attgaaaaga gatcatttat tgttaccaat agatcataat aaagtaccta atattaaaaa
     1621 tttagattca gattatatga atatgtcatt tgataaaggc aataaatatt ctttacctta
     1681 tttctttgga actgtaggta ttttatataa taaagaaaag tatccaaatg aatcatttga
     1741 tagttggaag tcattgtata atcctaaatt taaaaaccaa attttactag ttgacggtgc
     1801 tagagagatt ataggcatga gtttgaataa acttgggtat aatcttaatg accgtaattc
     1861 gcaccattta aaagaagcag agcgagattt aactaaatta gcaccacaag taagaggtgt
     1921 cgtaggtgat gaaattacca tgatgcttga acaaaatgaa ggtaacattg cggttgtttg
     1981 gagtggtgtt gcagcacctc tagtgcaaga aggggataaa tataactatg ttattcctaa
     2041 agaaggatcg aatttatggt tcgacaatat ggtaattcca aaaacggcac aaaataaaga
     2101 gggtgcatat aaatttatga attttttatt agatgctaaa aataacaagc aaaatactga
     2161 attcgtaggc tatgcaacgc caaacaaggc tgctcgacaa ttgttgccta aagagattaa
     2221 agacgaccat cgtttctatc cgactaagaa agagcaagaa cgccttgaag tttataaaga
     2281 tttaggacct gaagttttaa gtgaatacaa tgaaaacttt ttgaatttca aaatgtcatt
     2341 aaaataaaat cataaatagt agttatttac tgtatattta gttataataa cgggataagt
     2401 actataagga gttgtaacga atgactggag aacaatttac tcaaattaaa cgtccagtaa
     2461 gtagattaac tgaaaaagtt ctaggttggt tatgttgggt gatgttatta gtgcttactg
     2521 tcattacgat gtttatagca cttgtttcat tcagtaataa cacatcgatt gctaatcttg
     2581 aaaatacatt aaacaataat gcatttatcc agcaattatt agctggaaat ggctataata
     2641 caacacaatt tgtaatatgg ttacaaaatg gtatatgggc tattatcgtt tactttattg
     2701 tttgtttgtt gatttcattt ttagctctca tatctatgaa tataagaatc ttgtcaggtt
     2761 tcttattctt aatatcagca attgtaacga ttcctttagt tttacttatc gttactttaa
     2821 tcattccgat attattcttt attattgcga tgatgctatt tataagaaaa gataaagttg
     2881 aaatggttgc gccacaatat tatgaagagt acaatggacc gatttatgat tatcgtgaac
     2941 ctgtgtatga gcgcccacaa ccgaaagatg attattatga tgtgcctaaa tatgaaaaag
     3001 aattggataa atcaaacact gtatatgatc aagaactgga aagagataaa tatgatcaat
     3061 ttcctaaacg tgcagttgaa agtgaatata atcatgatga gcgcactgag gaagaaccat
     3121 cagtattatc cagacaggct aaatacaaac aaaaaagtac tgaagaacta ggtattgaag
     3181 atgatggtta ttatgcagaa cctgaagttg atccaaaaga attgaaagca caacaaaagc
     3241 gagaaaaagc tgaaattaaa gctaagaaaa aagaaaaacg taaagcttat aaccaacgaa
     3301 tgaaagaacg taggaaaaat caacctagcg cagttagtca acgtcgaatg aattttgaag
     3361 agcgacgtca aatttacaac aatgatattt ctgaagaacg caattcaagt gaagttaagg
     3421 acaaaaaaga gcaagaataa atattgatga tacccagatt agtaagtaga ggtcttttgc
     3481 ttattaatct gggtttttat atgaggttaa ttatcgataa cgtttaatta aagtgtttag
     3541 gtgtca
//
LOCUS       DATTQF010000191         3429 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.194, whole genome shotgun sequence.
ACCESSION   DATTQF010000191 DATTQF010000000
VERSION     DATTQF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.194"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..132)
                     /locus_tag="VB241_002548"
     CDS             complement(<1..132)
                     /locus_tag="VB241_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HEP1245765.1"
                     /translation="MTFEEKQSEMYNKIANEISGMIPVEWEKVYTIAYLDDEGGEVVF
                     "
     gene            complement(143..631)
                     /locus_tag="VB241_002549"
                     /pseudo
     CDS             complement(143..631)
                     /locus_tag="VB241_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            complement(642..1142)
                     /locus_tag="VB241_002550"
     CDS             complement(642..1142)
                     /locus_tag="VB241_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HEP1245766.1"
                     /translation="MTFEEKLSEMYNEIANKISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKINSDELNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRNLFKEEGHEPWTSCEF
                     DFTRDGKLNVSFDYIDWANSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QEEAEL"
     gene            complement(1153..1653)
                     /locus_tag="VB241_002551"
     CDS             complement(1153..1653)
                     /locus_tag="VB241_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HEP1245767.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPIEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQNLRNIFKEEGYEPWTSCEF
                     DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QDEAEQ"
     gene            complement(1785..2003)
                     /locus_tag="VB241_002552"
     CDS             complement(1785..2003)
                     /locus_tag="VB241_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245768.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLKIAIIVTIIVFVLNLLAYLGLR"
     gene            complement(2180..2767)
                     /locus_tag="VB241_002553"
     CDS             complement(2180..2767)
                     /locus_tag="VB241_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HEP1245769.1"
                     /translation="MIIFILLTVFALFYIAMIASLFKSEGFSIIGLILDIVILTTLIF
                     YYFVGASFVDNDLSNFLAFMNFGSFVYMYYAIKSLWVKPKLVNYIIAKEIGESKDVIE
                     EQELDLQTSKIRGIYFFIIAIALLIITKLRMQPELQADAISMNPVFIFIGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIVLS"
     gene            complement(2791..3249)
                     /locus_tag="VB241_002554"
     CDS             complement(2791..3249)
                     /locus_tag="VB241_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HEP1245770.1"
                     /translation="MDKNGYSYIMMKNVAYKTYFDVTMDDYEDILKDFTNLAISKGKT
                     ITGPLTFAVTQIDLQKRMNIDLFISVGKCFKSNEELSYRTYFCLDNMLHGRITSNNFI
                     TDEIALLEDMNEFASDNNLTFTSPYYHTMRKSFSGEQGWIDVKAKVYEND"
     gene            complement(3242..>3429)
                     /locus_tag="VB241_002555"
     CDS             complement(3242..>3429)
                     /locus_tag="VB241_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HEP1245771.1"
                     /translation="STKCFEEEEFERAYKEIEYVANENNIKILNQPYYHVMVDYFGGT
                     AFEIYAQVDLDESEVYG"
BASE COUNT         1113 a          662 c          323 g         1331 t
ORIGIN      
        1 aaaaacgact tctccacctt catcatctag gtaggcaatt gtatatacct tttcccactc
       61 tactggtatc atcccactaa tctcatttgc aattttattg tacatttcac tttgtttttc
      121 ttcgaaagtc atgttatctc ccctatagtt cagcttcttc ttgctcttta atatattgct
      181 cgatttcttt aatttcttcc atttctggta taaccccaaa ttttttgtac atataataat
      241 tttcaagact caattgatca aattctgtat tctttcaatc aatataatca aatgaaactt
      301 ttaatttacc ttcgtttgta aaatcaaatt cgcatgatgt ccatggttca tgtccttctt
      361 ctttaaatat atttcttaat tttttaaatg atctataaag ttccatccat aaatcatcaa
      421 atatttcttc cgaaacatta taatctctag atatattcat gtaataattc aattcatcgc
      481 tacctggttt agtataatta aaaacgacct ctccacctct atcatttaca taggctattg
      541 tatatacctt ttcccattct acaggtatca tgccaacaat ctcatttgca atcttgttgt
      601 acatttcact tagtttttct tcgaaagtca tgttatctcc cctatagttc agcttcttct
      661 tgctctttaa cataatcttt tattttttca acccaattta tggcatattc tttttcaggc
      721 caaattccaa attttttata catataataa tgttctcttc ccattggccc aaactctgaa
      781 ttcgcccaat caatataatc aaatgaaaca ttcaatttgc cgtctcttgt aaaatcaaat
      841 tcgcatgatg tccatggttc atgtccttct tctttaaata aatttcttaa ttcctcaaac
      901 aaatcatata aatccatcca taaatcatca aatacttgca cagaaatgtt atactcctta
      961 ggtatatcgg tgtaataatt caattcatcg ctgtttattt tagtataatt aaagaatact
     1021 tcacctcctc catcatctat ataagccatt gtatatacct tttcccattc tactggtatc
     1081 atgctactaa ttttattcgc aatttcattg tacatttcac ttaatttttc ttcgaaagtc
     1141 atgttatctc ccctattgtt cagcttcatc ttgctcttta atatattgct cgatttcttt
     1201 aacttcttcc atttcatatt ccatttctgg tataacccca aattttttgt acatataata
     1261 attttgacgg cccaattgat caaactctgt attgatccaa tctatataat caaatgaaac
     1321 ttttaattca ccttcttttg taaaatcaaa ttcgcatgat gtccatggtt catatccttc
     1381 ttctttaaat atatttctta aattttgaaa ttgtttatac aactcatacg ctgagtccat
     1441 aaattctgat tccgatatat catatttatt taatacacta gtatagtagt ataattcatt
     1501 gcttccaggt tctgtgtaat agtagaacac ttctccacct tcatcatcta tataagccat
     1561 tgtatatact ttttcccact ctatcggtat catcccactg atctcattcg caatctcgtt
     1621 gtacatttga cttaacttct cttcgaaagt catgttatcg cccctatgtg ttcgaaatat
     1681 ctcttgatta agctattaat ttattctcta cacttatttc cgttaaataa aaagcattaa
     1741 acaacaccca aatgtcgctt aatgcttctt caaattcatt ttacttatct taaacctaaa
     1801 tatgctaata aatttaaaac aaaaacaatt attgttacga taattgcaat tttcaataac
     1861 ttcgaacctt tttctgcatc gtattttttg taattaatac tacaaacaat acttccaata
     1921 ccaaatagcg gtgtaactac tgttaataca ataaataccc atgttgcaat ttttaagcca
     1981 ttgccttgtt tttggttttc catctcatta actcctactc attattttgt ctttcaattt
     2041 atttaactat ttataattat agtaaaaaag atattaatag tatatacttt ttacatattt
     2101 ttcaaaaatc attaagattt ttctatcgat taattaatat ccatcaacaa ttataaccaa
     2161 aaaaattatt gaaaatttct tatgaaagta caatagtagc tataataatc caagtcgtaa
     2221 caactaatgg cactatcgtc ttgaataaga atataccgta ttttttctta cgatatatat
     2281 ccagtactag ccaaattaaa atgataataa ctcctataaa aataaaaact ggattcatcg
     2341 atatagcatc tgcctgtaac tcaggttgca ttcttaattt agtaattatt aacaaggcaa
     2401 ttgcaataat gaaaaagtag atacctctta tctttgatgt ctgtaaatct aattcctgtt
     2461 cttcaatgac atcttttgat tcgcctattt cttttgcaat tatataattt actaacttag
     2521 gcttcaccca caagctctta attgcatagt acatatacac aaatgaccca aaattcatga
     2581 atgctaaaaa gttacttaaa tcattatcaa caaaactcgc acctacaaaa taataaaata
     2641 ttaacgttgt caaaataact atatccaata ttaagcctat tattgaaaaa ccttcacttt
     2701 taaataaact agcaatcatc gctatataaa agagtgcaaa aactgttaat aaaataaaaa
     2761 ttatcatata ttacctctac tgattattaa ttaatcattt tcatacactt tggcctttac
     2821 atcaatccaa ccttgttcac cgctaaaact ttttctcatt gtgtgataat aaggcgaagt
     2881 aaacgttaaa ttgttgtcag aagcaaattc gttcatatcc tcaagtaacg ctatttcatc
     2941 tgttataaaa ttgttggaag tgatacgacc atgtaacatg ttatctagac aaaaatatgt
     3001 tctatatgat agttcttcat ttgatttaaa acattttcca actgaaataa ataaatcaat
     3061 attcatcctt ttttgcaagt ctatttgcgt gactgcaaag gttaatggcc ctgtaattgt
     3121 tttaccttta gaaattgcaa gatttgtaaa gtcttttaat atatcttcat agtcatccat
     3181 agtcacatca aaataagttt tgtaagctac atttttcatc ataatatatg agtatccatt
     3241 tttatccata tacttcactc tcatccaaat ctacttgagc ataaatttca aaagccgtac
     3301 caccaaaata atctaccatt acatgataat atggttgatt taggatctta atattatttt
     3361 cgttagctac atattcaatt tctttataag ctctttcaaa ttcctcttct tcaaaacact
     3421 tagttgata
//
LOCUS       DATTQF010000192         3403 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.195, whole genome shotgun sequence.
ACCESSION   DATTQF010000192 DATTQF010000000
VERSION     DATTQF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.195"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            96..1076
                     /locus_tag="VB241_002556"
     CDS             96..1076
                     /locus_tag="VB241_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEP1245772.1"
                     /translation="MEDLEKVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLVALVSVAESVEKPILSQEINVVAT
                     LKLLETIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAVGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHDFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            1039..1728
                     /locus_tag="VB241_002557"
     CDS             1039..1728
                     /locus_tag="VB241_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEP1245773.1"
                     /translation="MKKLQRKKWKCREIGIEAVRNKGYAYQRKLDTLEEVKKGYFPIK
                     RAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMCK
                     NAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVEL
                     FSSEIIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIRT
                     LMVVITGEGSR"
     gene            1938..3104
                     /locus_tag="VB241_002558"
     CDS             1938..3104
                     /locus_tag="VB241_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP1245774.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVSPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINDSTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICIV
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3085..>3403
                     /locus_tag="VB241_002559"
     CDS             3085..>3403
                     /locus_tag="VB241_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HEP1245775.1"
                     /translation="MENQHNSKYLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFY"
BASE COUNT         1170 a          469 c          631 g         1133 t
ORIGIN      
        1 aaagttatcg cattttattt ttataaataa aaaataccga taaaaagctt acaatacttt
       61 atgtttttgt gatatatttt taatgtataa atgaggtgga agatttggaa aaagttttga
      121 taactggtgg ggctggtttt attgggtcac atttagtaga tgatttgcaa caagattatg
      181 atgtttatgt tctagataac tatagaacag gtaaacgaga aaatattaaa agtttggctg
      241 acgatcatgt gtttgaatta gatattcgtg aatatgatgc agttgaacaa atcatgaaga
      301 catatcaatt tgattatgtt attcatttag tagcattagt tagtgtcgct gagtcggttg
      361 agaaacctat cttatctcaa gaaataaacg tcgtagcaac gttgaaatta ttagaaacca
      421 ttaaaaaata taacagtcat ataaaacgat tcatctttgc atcatctgca gccgtttatg
      481 gtgatttacc tgatttacct aaaagtgatc aatcattaat tctaccatta tcaccatatg
      541 caatagataa atattacggc gaacgtacga cattaaatta ttgctcgcta tataacattc
      601 caacagctgt tgttaaattc tttaatgtat ttgggccaag gcaagatcct aagtctcaat
      661 attcaggtgt gatttcaaaa atgttcgatt catttgaaca taacaagcct tttacatttt
      721 ttggcgatgg tctgcaaacg agagactttg tttatgttta tgatgttgtt caatctgtgc
      781 gcttaattat ggagcataaa gatgcggttg gacatggtta taacattggt acgggtacat
      841 ttacgaattt gctagaggtt tatcgcatta ttggtgaatt atatgggaaa tcagtcgagc
      901 atgattttaa agaagcaaga aagggagata ttaagcattc ttatgcagat atttctaatt
      961 taaaagcatt aggatttgtt cctaaataca ctgtggaaac aggcttaaag gattacttta
     1021 attttgaggt agataatatt gaagaagtta cagcgaaaga agtggaaatg tcgtgaaatt
     1081 ggcattgaag ctgtccgtaa taagggttac gcctatcaaa gaaaattaga cacactagaa
     1141 gaagtaaaaa agggctattt tccaatcaaa cgtgcgattg acttggtatt aagtatcgtt
     1201 ttattatttt taacatttcc gattatgttc atattcgcca ttgctatcgt catagattcg
     1261 ccaggaaacc ctatttatag tcaggttaga gttgggaaga tgggtaaatt aattaaaata
     1321 tacaaattac gttcgatgtg taaaaacgca gagaaaaacg gtgcgcaatg ggctgataaa
     1381 gatgatgatc gtataacaaa tgtcgggaag tttattcgta aaacacgcat tgatgaatta
     1441 ccacaactaa ttaatgttgt taaaggggaa atgagtttta ttggaccacg cccggaacgt
     1501 ccagaatttg tagaattatt tagttcagaa ataataggat ttgagcaaag atgtcttgtt
     1561 acaccaggat taacaggtct tgcgcaaatt caaggtggat atgacttaac accacaacaa
     1621 aaattaaaat atgacatgaa atatatacat aaaggtagct taatgatgga attatatata
     1681 tcaattagaa cattgatggt tgttattaca ggggaaggct caaggtagtc ttaatttgct
     1741 taataagttt aaataaaagt tacattttaa agattgtgac caattgttac agtataacga
     1801 ggaatccctt gagacagtat caaatggcat taagaaatat ttgccatcat tgatttgcat
     1861 ggctataaat actattcatc tgatgagata gccatgttaa gaaattgaaa gtatagcatt
     1921 aaaggggttt gtaacagttg aaaattatat attgtattac taaagcagat aatggtggtg
     1981 cacaaacaca tctcattcaa ctcgctaacc atttttgcgt acaccatgat gtttatgtca
     2041 ttgtaggcaa tcatggacca atgattgaac aactagatgc aagagtcaat gtaattatta
     2101 tcgaacattt agtaagtcca attgacttta aacaagatat tttagctgtc aaagtgttag
     2161 cacagttatt ctcgaaaatt aaacctgatg ttatccatct acattcttcc aaagctggaa
     2221 cggtcggacg aattgcgaag ttcatttcga aatcgaaaga cacacgtgta gtttttactg
     2281 cgcatggatg ggcttttaca gagggtgtta aaccagctaa aaaatttcta tatctagtta
     2341 ttgaaaaatt aatgtcacgt attacagata gcattatttg tgtttcagat ttcgataaac
     2401 agttagcgtt aaaatatcga tttaatcgat tgaaattaac cactatacat aatggaattg
     2461 cagatgttcc cgctgttaag caaacgctaa aaagccaatc acataacaat attggcgaag
     2521 ttgttggaat gttgcctaat aaacaagatt tacagattaa tgactcgaca aaacatcaat
     2581 ttgttatgat tgcaagattt gcttatccaa aattgccaca aaatctaatc gcggcaatag
     2641 agatattgaa attacataac agtaatcatg cgcattttac atttataggc gatggaccta
     2701 cattaaatga ttgtcagcaa caagttgtac aagctgggtt agaaaatgat gtcacatttt
     2761 tgggcaatgt cattaatgcg agtcatttat tatcacaata cgatacgttt attttaataa
     2821 gtaagcatga aggtttgcca attagcatta tcgaagcgat ggctacaggg ttgcctgtca
     2881 tcgctagtca tgttggtggc atttcggaat tagtaactga taatggtata tgtatagtga
     2941 acaatcaacc tgaaactatt gccaaagtcc tggaaaaata tttaatagac agtgattaca
     3001 tcaaaatgag taaccaatct agaaaacgtt atttagaatg ttttacagag gagaaaatga
     3061 ttaaagaagt ggaagacgtt tataatggaa aatcaacaca atagtaaata tctaacattg
     3121 ttacttatcg gtttagcggt ttttattcag caatcttcgg ttattgccgg tgtgaatgtt
     3181 tctatagctg actttatcac attactaata ttagtttatt tactgttttt cgccaaccat
     3241 ttattaaagg caaatcattt tttacagttt ttcattattt tgtatacata tcgtatgatt
     3301 attacgcttt gtttactatt ttttgatgat ttgatattta ttacggttaa ggaagttctt
     3361 gcatctacag ttaaatatgc atttgtagtc atttatttct att
//
LOCUS       DATTQF010000193         3237 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.197, whole genome shotgun sequence.
ACCESSION   DATTQF010000193 DATTQF010000000
VERSION     DATTQF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.197"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1164
                     /locus_tag="VB241_002560"
     CDS             <1..1164
                     /locus_tag="VB241_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEP1245776.1"
                     /translation="NARKELKNWTKTKNVDTPLYLFPTKSYIKKEPYGTVLIIAPFNY
                     PFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDANYIEVIEGGIEETQ
                     TLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVIVDETANIKVASERI
                     CFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNIQQSPDYGRIVNLKH
                     YHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTNDSAIMQEEIFGPILPILTYQ
                     SLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAINDTLMHLANPKLPFGG
                     VGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKFKYIKAFFKN"
     gene            complement(1284..2312)
                     /locus_tag="VB241_002561"
     CDS             complement(1284..2312)
                     /locus_tag="VB241_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEP1245777.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            2583..2984
                     /locus_tag="VB241_002562"
     CDS             2583..2984
                     /locus_tag="VB241_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HEP1245778.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(3041..3214)
                     /locus_tag="VB241_002563"
     CDS             complement(3041..3214)
                     /locus_tag="VB241_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245779.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         1096 a          583 c          475 g         1083 t
ORIGIN      
        1 aatgcccgta aggaacttaa aaactggact aaaaccaaaa atgtagacac acctttatat
       61 ttatttccaa caaaaagcta tatcaaaaaa gaaccttatg gaacagtttt gatcattgca
      121 ccatttaact atccttttca actagtgttc gaacctttaa tcggtgctat tgcagcaggt
      181 aatacagcaa ttattaaacc atctgagttg acaccaaatg ttgcacgagt gattaaacga
      241 ttaatcaatg aaacatttga tgcaaattac attgaagtta ttgaaggagg aattgaagaa
      301 acgcaaacgt taattcactt accttttgac tatgtcttct ttacaggaag tgaaaatgta
      361 ggcaaaatcg tttatcaagc tgccagcgaa aatttagttc ctgtgacatt agaaatgggc
      421 ggtaaatctc cagtcatcgt tgatgaaaca gcgaatatta aagttgccag tgagcgcatt
      481 tgttttggga aattcaccaa tgccggccaa acatgtgttg caccagatta cattttagta
      541 cacgaatctg taaaagatga tttaatcaca gccctatcaa aaacgttgcg tgaattttat
      601 ggtcaaaata tacaacaaag tccagattat ggccgcattg taaaccttaa acattatcat
      661 cgtctgacgt cattacttaa cagtgcacaa atgaatattg tatttggtgg tcatagtgat
      721 gaggatgaac gttatataga accaacattg ttagatcacg ttacaaatga ttcagcaatt
      781 atgcaagaag aaatttttgg tcctatctta ccgattttaa cgtatcagtc attggatgaa
      841 gcaatagcct ttattcacca aagaccaaaa cctttgagtt tatatttatt tagcgaagat
      901 gaaaatgcta cgcagcgtgt aataaacgag ttatcatttg gcggcggcgc tattaatgat
      961 acattgatgc acctagcgaa tcctaaatta ccatttggtg gtgttggcgc ctcaggtatg
     1021 ggacgctatc atggtaaata ttcatttgac acttttacac atgaaaaaag ctacattttc
     1081 aaatctacac gattagaatc aggtgtccat ttaccaccat ataaaggtaa atttaaatac
     1141 atcaaagctt tctttaaaaa ttaatttttg agacaaaaat aaaccatgct tccataattt
     1201 gatgttcttg aaaacaataa atttcaaggt atacacaaat tttttggaaa catggttttc
     1261 tttttatcat gattaattaa agattaaaat atgacacata caccttcaga tgctacacga
     1321 gtgttatcac atagcgtaat tttacctgtt tctttatttc tttcgaaaac agttacaact
     1381 gaatctcctt gctcatgagc acaaactaaa aggtcatctg atgaggcaat attaaaatct
     1441 cttgggaatt gaccaccact ttcagtaatt gttactagtt ctaagtgttg accattatca
     1501 agaactttaa aaattgcaat gctatcatgc cctctattag atacatataa gaattgttga
     1561 tcatgagaca aacgcactgc tgcaagttta gtatctccat caaagttttc aggaattgtt
     1621 aaatgacgct cgagctcttc aaatttaccg tcattatatt ctgcaacact cacagtattg
     1681 gataattcgt gtacgacata agcaaatttt ccattatcat gaaattcaat atgtcttgtc
     1741 ccatcactat ctttaaataa agattcttta taaaattcaa acccgttgtc atcaaattta
     1801 taagtaacga tacgatcagc acctaaatct gttactgcaa cgtacttacc atctggagtt
     1861 tgattaatat aatgtgcgtg tggatgatct tgtctttcat gtgtacctgt tggaaaatca
     1921 tgcgcaagtt cttgaattag acgtataatt tcacctgtgt gcgtgtttaa ttcatacata
     1981 cgtatgatgc cagctccata taccgcttca aataaatatc gtttatcttc tgaaatcgat
     2041 acataacaac ctgtaccagc ttttgaagac aaacatttat ttaataaatg caattcacca
     2101 ttgtcatcaa ttttcaaact cgcaacaccg cattgttctc cttctttgtt gattccatat
     2161 aaaacttcgt tattacgcac caaatatgta gacgcttcta attcaaatcc tgtttctaat
     2221 aaatcaatac gtgactgatt ttcgtttaat tcaaaacgat aaatcccttt accattcttt
     2281 ttagtgtaag aaccaatata tccatttgtc attactttta cccctccatg ttagtcttat
     2341 tttcatttta ttaaatttta gctagaatta acaatcattt atcatttcct taaagctaaa
     2401 actgtttaac tactttaaaa gaactatttg ctggtgatgt gtcattattc gattttaatt
     2461 aatccctctt ttttacttac ccgagcatta attattaacg cgtttatgct tccacaaaat
     2521 ttttgttgca aaatagaaca cgtgttcgta taatgtaatt atcagattgg ggtgaattaa
     2581 ggatgacaag ttatgaagaa gaaactgatt atcgtaacgt tccaagagaa ttactagata
     2641 acaatatacc tatgggaaga ggcatgatta aatgggctcc ttttgcaaca ttacccgaac
     2701 aattcgaaac gattcaacaa tacattatcg accaaaacaa aattacacgt cctgtattat
     2761 cagatgatca attggctgag ctaaacattc ggctacacga ggcgctacaa tatgcccaac
     2821 ctgtagaagt taaattttat aataatggct ttgtagattc agtacgctta accatttatc
     2881 gtattgatgc tattaattat gaaattgatg gttatgttta caatcagcaa caacgtcaaa
     2941 aaatttctat ctttgatatc ttagatattg tcttgttacc ttaatgcttt gaacataatt
     3001 aaaggcaaga acaatcaata ttgtccttgc ctaaatgtta ttagttattt ttactttcga
     3061 tatcagaaat aatttcttta gtttttcttt caatatcttc aaaatctttt ttaaagatat
     3121 aagcaaatgc tactacacca acaccagcaa ctaatgttgt aatagcaact aagctaaata
     3181 cgaatttaaa cacacattta ataaaattcc acatctcaat ttcacctctg ttaaatt
//
LOCUS       DATTQF010000194         3179 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.198, whole genome shotgun sequence.
ACCESSION   DATTQF010000194 DATTQF010000000
VERSION     DATTQF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.198"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..245
                     /locus_tag="VB241_002564"
     CDS             <1..245
                     /locus_tag="VB241_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1245780.1"
                     /translation="KIYSQRKIDVEPVFGFMKAILGFTRMSVRGINKVKRELGFVLMA
                     LNIRKIVARRAVYYQIHLKKADFYQIINRNQLFYIA"
     gene            complement(317..1633)
                     /locus_tag="VB241_002565"
     CDS             complement(317..1633)
                     /locus_tag="VB241_002565"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HEP1245781.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYVIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNCQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1685..2509)
                     /locus_tag="VB241_002566"
     CDS             complement(1685..2509)
                     /locus_tag="VB241_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP1245782.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIERNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2623..2931
                     /locus_tag="VB241_002567"
     CDS             2623..2931
                     /locus_tag="VB241_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP1245783.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT          992 a          527 c          528 g         1132 t
ORIGIN      
        1 aaaaaatcta tagtcaaaga aaaattgatg tagagcctgt ttttggattt atgaaggcta
       61 ttttgggttt cactcgaatg tcagttcgag gaataaataa agttaaacga gagctaggtt
      121 ttgtattaat ggcacttaat ataaggaaaa tagtagctcg acgagctgta tattatcaaa
      181 tacatttaaa aaaagctgat ttctatcaaa taattaatag aaatcagctt ttttacattg
      241 cctaagaact taatgtccca agctcaacaa aattctaacc tagacaatta agattagatt
      301 gaatcgcatg attcatttat ttagctttgt aaccaatcat attgattaaa tctttagggt
      361 ggctatatgg tggtgcataa gccacttcaa actcagttaa ctcatctaca gttagctggt
      421 tcatcattgc catcgatagt acatcaatac gtttatctgc gccttctttt cctactgcag
      481 ctgctcttaa aatctgacag tttgaagtgt cataatatac tcttaagtgt aaaggggaat
      541 ttcctgggta ataattcgcg tgtgcacctt gagtgacttc caccatttta tagtcaaatt
      601 gctttagttc gtttggttta acgccgacac tcgcaaatgt ataatcaaag aacttcacaa
      661 tattgttgcc taagaagcct ttgaattcaa tagtgtcatt tccagcaatt tgttcggcaa
      721 caatacttgc tgcacggtga gcgccccaag ctaaaggaac actagccggt agatcgacat
      781 gtcgataatg tgatgttgca atatcgccta ttacataaat gtttggaaca tttgtttcaa
      841 atttatcgtt taccggtatg aaaccttttc gatcaagttt gatatttgaa ctttcgataa
      901 attttgaatt ggggtgagta ccgacacctt caataatcat atcgtaatgt tcaacttttc
      961 ctgatttaaa tgtaatttca tttccattga tagcatcaat ttcctcattt aaacggtatg
     1021 gaatctcccg cttatctaat tcatcaagta taggttgatt catatcggca tccattaatt
     1081 tatttatctt atcagatcga tgaattaaag tagggtgtaa accacgttca taaagatttt
     1141 caagaacttc taatgaaaca taccctgcac ctacaaccaa tactttatca acttgatttg
     1201 ctttgatgaa ttgatcgata gcatcagtgt cttctaaatt tctaagtgta aatgtaatat
     1261 cactttcaaa gccaaggcta tttgcacttg caccagggct taaaatgagt ttatcgtatg
     1321 attcttcaaa ttgttcgttg gtctttctat ttaatacaga tacagtttgt ctttcatcat
     1381 tgattgcaat aacttcatga taagttttta ctgtaatttg ttttctatca taaaattttt
     1441 caggtgtata cgctaaagca tattttctat cttcaacaac ttcgccaatg acataaggca
     1501 atgcacaatt agcaaagctc atatcacgat ctttttcaaa aataataatg tcactttctt
     1561 tatctaaacg tcgaatttgg ctggcacatg ttgctccgcc agcgactgct ccgactacga
     1621 ctattttggg catcgtaact tacctcctat tgttttaaaa tattataaaa tgattagtta
     1681 taaattaaca gtaatatcta atatttaaat taaagaaatc atttaaatat cgaccaattc
     1741 catcttcatt attgttgaat gtaatattgt tcgctacatc tttaagttct tgcaaaccat
     1801 tttccatagc aacaccgtga cgcgcgtact caatcatttc tatatcatta tcttcatcac
     1861 cgaatgcaat aatattattt cgttcaatat ttaaaaattg tctaacttgt tcaatgcctc
     1921 ttgctttatt aattccaagt tttacaattt caatgacagg gaatggtgcg ccccagcgtc
     1981 gatgctcaat atgatcggca taaaaatgag taagcatatt tttgatttca ggtattttac
     2041 tttcttcggc ttcaattaaa attgaggtag gggattcttt caagtggaca agtaaattac
     2101 cagtttgaat tcttggatta cccattgaaa aaccttcaaa taatcttgga tcatgattat
     2161 taatgaaaac ataatctttc acttctgcaa taatattcga tacttgatat tgttgtaatc
     2221 cttgaataat attttgtgcg atgcctaaat ctaaaatttc atggcaagtt ttgaagtttt
     2281 tatctttagg gtgatgtacg taagcgccat taaaattaac aattggtgtc gttaaattta
     2341 attcatgata atacatttga cttgcacgat aaggtctgcc agtcgcaatc ataatttggt
     2401 gtccacgttg ttgtaattca tttaatactt gtttagtata tgatgaaatt tctttgttat
     2461 cgtttaataa tgttccgtct aagtctagac atattaaatg tggttgcata tctctctcct
     2521 cataagttgc ttagttcata tagatacatc atagcatttt attggcgttt tgtcggatat
     2581 ttgatatatt atgaataata tgacattaaa gaggtgatat ggatggaaga ggcattgaaa
     2641 gatagtatct taggtgcatt agaaatggta attgaccctg aattaggaat tgatatcgtt
     2701 aatttaggtt tagtatacaa agtgaatgtt gatgatgaag gcgtatgtac agttgatatg
     2761 actttaacat caatgggatg tccaatggga cctcaaatta ttgatcaagt taaaacagta
     2821 ttagcagaga ttcctgaaat tcaggatact gaagtgaata tcgtatggag tccaccttgg
     2881 acaaaagata tgatgtcacg ttacgctaag attgcacttg gtgtgagcta acataaactt
     2941 gcattacttg tagaaattgg aaatccaatt ggggctccgc tcccaacttg cattgcttgt
     3001 agaatttctt ttcgaaattc tctgtgttgg ggccccgcag tcaatataac attgtagagt
     3061 ataattcatt gtgaataagc tcccttcaaa gtagacattg aagggagcct tatttacgtt
     3121 ctaggttctg actttatttt gaaaatttat atctaacttg tttcagaaaa tatgaatta
//
LOCUS       DATTQF010000195         3081 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.199, whole genome shotgun sequence.
ACCESSION   DATTQF010000195 DATTQF010000000
VERSION     DATTQF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.199"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..231)
                     /locus_tag="VB241_002568"
     CDS             complement(<1..231)
                     /locus_tag="VB241_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1245784.1"
                     /translation="MTKFQYKKIIDDIINKINNGILSPGDKLYSQRKLAKYYNVNKST
                     VIQALDILKSYGILDTIEKKGIYVSQYKWNSYI"
     gene            330..1310
                     /locus_tag="VB241_002569"
     CDS             330..1310
                     /locus_tag="VB241_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEP1245785.1"
                     /translation="MTLKLEKAVFSMEIKKDLKLLMKKDNYHNIFALIFDWLVILGSA
                     CISIYMSNLIIYIISIILIGSRMRAFDNLMHEACHRSLFTNKFYNKWIACLLVAFPIF
                     TSFTTYCNSHFKHHRNLWDEEKDPDTKRYRIVGLDKPQKDVKKFIINHIVKVLFLFHV
                     PKYVLGTVSANLYSRDTPKSEIWTRNIFWVTIITLSIMFNFWLYVILYWFIPLLTTFQ
                     IIRYWAEMAEHSGLNNDSELTASRNTFGMPWTIFLFHPHHDNFHLVHHLFPAIPHYNL
                     KKAHIILMKDQKYREAHHCTGFFKTTLPGFYSVVKDICTPNIVGKGEDAN"
     gene            1307..1924
                     /locus_tag="VB241_002570"
     CDS             1307..1924
                     /locus_tag="VB241_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEP1245786.1"
                     /translation="MIQPVLHGILLALGLILPLGAQNVFVFNQGANQKKMINALPVVI
                     TAGLCDTFLIIIAVLGVSLILMSLPVLQLFIYIVGLFFLLYMAWSLWKEKPNTLENYE
                     PMSTKKQIGFAISVSLLNPHAIMDTVGVIGSSASVYSGLEKILFCIATIMVSWLWFFL
                     LAILGKVLGSIDRTGKYILFLNKISSIIIIAVSIVIVKNILKLIL"
     gene            complement(2155..2766)
                     /locus_tag="VB241_002571"
     CDS             complement(2155..2766)
                     /locus_tag="VB241_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEP1245787.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
BASE COUNT         1109 a          407 c          488 g         1077 t
ORIGIN      
        1 aatataagaa ttccatttat actgggatac ataaatacct tttttttcaa ttgtatctaa
       61 gatgccatag ctttttagaa tatctaaagc ttgtataact gtggatttat ttacattata
      121 gtacttagct aattttcttt gcgagtacaa tttatctcca ggagataaaa taccgttgtt
      181 aattttatta ataatatcat caataatttt tttatattga aatttagtca taaatgtcat
      241 cctcaatctg gttggaattt ttattagaat ctggtaggtt ttttaatttt cagattatta
      301 taacatatta aataagggag gcggttaaat tgacactaaa attagaaaaa gctgtatttt
      361 caatggagat aaaaaaagat ttgaagttat taatgaaaaa ggataattac cacaatattt
      421 tcgctttgat ttttgactgg ttggtaattc tcgggagtgc atgtataagt atctatatga
      481 gtaatctcat tatttatatt ataagtatta ttttaattgg aagtcgaatg agggcgtttg
      541 ataacttaat gcacgaagca tgtcatcgtt cattatttac aaataagttt tataataaat
      601 ggattgcttg tcttttagta gcattcccta tatttacaag ctttactaca tattgtaatt
      661 ctcattttaa acatcataga aatttatggg atgaggaaaa agacccagat actaaacgat
      721 atcgcattgt aggtttagat aagccgcaaa aagatgtgaa aaaatttata attaatcaca
      781 ttgtaaaggt actattttta ttccatgtgc ctaaatatgt tttaggtaca gtttcagcta
      841 acttatacag tagggataca cctaaatcag aaatatggac tagaaatatt ttctgggtaa
      901 caattattac gttatcaatt atgtttaatt tttggttata tgttattctt tattggttta
      961 taccactttt aacaactttt cagattattc gttattgggc agaaatggct gaacactctg
     1021 gattgaataa tgatagtgag ttaacagcaa gtcggaatac ttttggtatg ccttggacaa
     1081 tatttttatt tcatcctcac catgataatt ttcacttagt tcaccattta ttcccagcaa
     1141 ttccacatta taatttgaaa aaagcacata ttatattaat gaaagatcaa aaatatagag
     1201 aagcacacca ttgtactggg ttcttcaaga cgacactacc gggtttttat agtgtagtaa
     1261 aagatatttg tacaccgaac attgttggga agggggagga tgcgaattga tacaaccagt
     1321 tttacacggt attttattag cattggggct tattttgccc ctaggagcgc aaaacgtgtt
     1381 tgtatttaat caaggtgcta atcaaaaaaa aatgataaac gcattgccgg ttgttataac
     1441 ggctggatta tgtgacactt ttttaataat aattgctgta ctgggtgttt cactaatatt
     1501 aatgtcatta ccagttctac aattatttat ttatatagtt ggtttgtttt ttctattgta
     1561 catggcttgg tctttatgga aagaaaagcc taacactcta gagaactatg aaccgatgag
     1621 tacaaaaaag caaataggtt ttgctatatc tgtttcgttg ttaaatccac atgcaattat
     1681 ggatacagtt ggtgtaattg gatcaagcgc atctgtctat tctggtttag aaaagatact
     1741 tttttgtatt gccacaatta tggtttcttg gctatggttt tttctgttag caatattagg
     1801 taaggtttta ggttctatag atagaacagg taaatatata ttatttttaa ataaaatctc
     1861 tagtataata ataattgctg ttagtattgt tattgtaaaa aatatattaa agttaatttt
     1921 ataatataaa agtgcatatt agttataatt ggaaaaacag taagtgatat ttaaaaatga
     1981 aaagatttta atattataat taatcttgag gtgttgagct ccttagaatt agttgacaaa
     2041 aaagattgga aatcttaact gaaaaaatgt aacattatta tgaaattata taaaaatata
     2101 aagaagaacc tcggaggaat aaattcgagg ttcttcttta tgctaacaac aatattattt
     2161 tactctctta cgtttgccaa tgtaacgtat agcaaagtaa atgactgcga taatgatgac
     2221 aacatacata atacgtgaat atgtatgaag accagtcatc aacataccaa agctatcgct
     2281 caacgtgcgt cctagtaaaa ttaaaccgaa gttccaaatt gttgtaccta ttaatgaaat
     2341 aacggtaaat gtcacaacat tcatgcggtt tacaccggct ggaatggtaa tcaatacacg
     2401 taatacaggt ataaaacgac agataaatac agcccatacg ccatactttt taaaccaatc
     2461 atttgctcgc tttaaatcct tacttttcaa tttaatccac ttaccgtgtc gatcaataaa
     2521 gcgatataga cgttcttctg aaatcaaacg acagatataa tataaaatta acagtcctat
     2581 aaacgatgcg atggttgcaa taataaataa ggttaaaatt gataaatgtg atttaacaga
     2641 cataagacca gcaaatgtta gaataatttc cgatggtacg ataggtaaaa cattttctaa
     2701 taaaattaat ataaaaatgg ctgcataccc aaaacgacta ataaattcag tgataatttg
     2761 ttccattatt aaaaataagc tcctttaatt ttcagcaatt gcagacaaat agaaactgga
     2821 taaattcaaa tgaataatga atgatgtcgt atgcgatgtt gaaacaagca tttgtcttaa
     2881 aatgtattat aagcaataaa tcaaaaaatt gcttgctata agtacgaaaa gtcaacaaat
     2941 gatcatctaa ataacaggcg ctgcacaatg aatgagtaat gtagttttat gcaatttaaa
     3001 atgaatgtca aatatgtaag aaatcctgca atatacgttt taaaacacta gtattgcagt
     3061 atttttaatt aacaaatgat a
//
LOCUS       DATTQF010000196         3065 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.200, whole genome shotgun sequence.
ACCESSION   DATTQF010000196 DATTQF010000000
VERSION     DATTQF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.200"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(149..544)
                     /locus_tag="VB241_002572"
     CDS             complement(149..544)
                     /locus_tag="VB241_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HEP1245788.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
     gene            complement(562..1854)
                     /gene="arsB"
                     /locus_tag="VB241_002573"
     CDS             complement(562..1854)
                     /gene="arsB"
                     /locus_tag="VB241_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HEP1245789.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVISPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGIGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            complement(1851..2165)
                     /gene="arsR"
                     /locus_tag="VB241_002574"
     CDS             complement(1851..2165)
                     /gene="arsR"
                     /locus_tag="VB241_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HEP1245790.1"
                     /translation="MSYKELSTILKILSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVESG
                     DC"
     gene            complement(2226..2561)
                     /locus_tag="VB241_002575"
     CDS             complement(2226..2561)
                     /locus_tag="VB241_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP1245791.1"
                     /translation="MITSLPMMNEVIGNPLLDKFMKDLIIQILAMISEQERNESKRRQ
                     AQGIQVAKEKGIYKGRPILYSPNAKDPQKRLVYYRVVELLEQGKSISTIAKEVGITRQ
                     TIYRIKNSK"
     gene            2881..>3065
                     /locus_tag="VB241_002576"
     CDS             2881..>3065
                     /locus_tag="VB241_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245792.1"
                     /translation="MNKKNELDIAFIMSTVLNVLFLVGLTFLVQLENLYILIPYTILG
                     FINAVYLTVRAINITKN"
BASE COUNT         1064 a          521 c          443 g         1037 t
ORIGIN      
        1 caaataatta actgtattaa tactctcatc atgattgcgt tataaacgtt cgttaatgag
       61 aaaaagagag ggcatattgt atatggcaca aattttgttc tttaaatggc aaaacacctc
      121 ttattaacta aataagaggt gtttatgatt atctcaattt aaacttttct atagctaatt
      181 taatctcgtc tctaacacgt tggaattctg accattcttt acctgctgga tcatcaaaac
      241 cccaatgctc ttttttaacg tttggtggta aaataggaca attattgtct gcatcactac
      301 ataacgttac gaccaaatct gattgtttta aaatatcatt atcaatcaag tctgacgtat
      361 ggtttgatat atcaatatct acttctttca tagcttctat tgctttagga ttaacaccat
      421 gtgtttcaat accagcagaa tagacattcc aaccttcacc caatatttcc tttccccaac
      481 cttcagccat ttggctacga caagagtttc ctgtacatat aaaataaatt gttttcttat
      541 ccataattaa agcccctttt cttaaaatat gattagtgta aggtataaac ctaagagtgt
      601 tacaaatagg actggaatag tgatgataat tccagtttta aagtatgttc cccacgaaat
      661 cttcacacct ttttgtgtta agacatgtag ccacaataat gttgctaaag aaccaattgg
      721 cgtaatttta ggtcctaaat cagaacctat gacattcgca taaaccatgc cttcttttaa
      781 tattcctgta gcactggatt ggccaatcgc tattgcatct attaaaacag ttggcatgtt
      841 gttcataatc gaagataaga aagcagctat aaagccgata cccatgatgc tgctaaataa
      901 cccataattt gaaatgtttg ttaaaacatc cccaaggacc gttgtaatac ctacattttt
      961 tagtccaaac acaacaagat acataccaat agagaataca acgatattcc atggtgcacc
     1021 tttaatcact tgttttgtat gaactgcttt agatttacga gctaatatta caaagataaa
     1081 agcaataata ccagcaataa ttgatacagg aatttggata aactcgctaa caagatatcc
     1141 aacgagtagt actgctaata ctatccatga aagcttaaat aacttagaat ctttaattgc
     1201 acttttagga tctgatagat tttctgtatc gaacgtttta ggtatggatt ttctgaaata
     1261 taaccataaa acgagaatac tagcaatcag agagaatata ttaggaatga tcattcggct
     1321 aaaatattca ataaatccaa tatcgaagta atctgcagaa acgatattaa ctaagttact
     1381 tacaattaag ggaagtgatg tagtatcagc aataaaacca ctggcaataa taaaggggga
     1441 aatcactttt tgattaaatc ctagattcct taccatcgct aatacaatag gcgttaagat
     1501 taaagctgca ccatcatttg cgaaaaatgc tgctacaatt gctccaagta acataataaa
     1561 aacaaacatt tttaagccat taccgtttga agccttgacc atatgtatcg cagaccattc
     1621 aaaaaaacca atttcatcta atattaatga aataagaata acagccacaa aagttaaagt
     1681 tgcattccaa acaatacctg ttacttctaa tacatcagaa agacttacga ctcctgtaat
     1741 gatagcaaca acagctccaa ttaaagctgt aataccaata tctaaacctt ttggttgcca
     1801 aatcacaaag gttaaagtta aaagaaaaat tacaattgct aaaatagtca tcaacagtca
     1861 cctgattcca catttttaca tacacatctt tgattagatg tattaatgat gttcaagttt
     1921 tggataatat catctaaaat agcatgatta agttgatacc aatgtttatt gccgtctttt
     1981 cgtgttgtaa ctaattcatt atctactaat gacttcatat gatgacttag tgtaggttgt
     2041 gagaattgaa agtgttctaa taagtcacaa gcgcatagct caccacaaga aagtaaatct
     2101 aatatttcta acctacttga atctgataaa atttttaata ttgttgatag ttctttataa
     2161 gacatgaata aacctcctag acattaaata gattaacatc tatatagatt attgtctatg
     2221 ttattttatt tactattttt tattctatat atagtttgac gtgtaatacc aacttcttta
     2281 gctatagtac ttatagattt accctgttca agtaattcaa caactcggta ataaactaaa
     2341 cgcttttgtg ggtctttagc attgggagaa tacagaatag gtctcccttt atatatacct
     2401 ttttctttgg cgacttgaat cccttgagct tgtcgacgtt tactttcatt tctttcttgt
     2461 tctgaaatca ttgctaaaat ttgaataatt aaatctttca taaatttatc tagtagtgga
     2521 tttccaatga cttcattcat cataggtaaa ctcgttatca tgagctgaac ttcttttcga
     2581 agatttttcg tagatataat ttgctaataa acttcctaaa actgaataaa gaaaaatagt
     2641 taaactcatg taatcactcc ataattctga agtgtctcca tgtctacgca tagacacgca
     2701 tttatgccga gaaaatttat tgtgcgttga gaagaaccct taactaaact tgcagacgaa
     2761 tgtcggcata gcgtgagcta ttaagccgac cattcgacaa gttttgggat tgttaagggt
     2821 tccgaggctc aacatcaata aagcaactat ggtaaaattt taaaaaagga ggaatgtatt
     2881 ttgaataaaa aaaatgagtt agatatagct tttattatgt ctaccgtttt gaatgtttta
     2941 tttttagttg ggttaacttt cttagttcaa ttagaaaatt tatatatttt aattccatat
     3001 actattttgg gttttataaa tgcagtttat ctaactgtta gggctataaa tattactaaa
     3061 aatag
//
LOCUS       DATTQF010000197         3025 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.201, whole genome shotgun sequence.
ACCESSION   DATTQF010000197 DATTQF010000000
VERSION     DATTQF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.201"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            16..231
                     /locus_tag="VB241_002577"
     CDS             16..231
                     /locus_tag="VB241_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEP1245793.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIIVIFTKKADKDGYIIFPKN"
     gene            295..426
                     /locus_tag="VB241_002578"
     CDS             295..426
                     /locus_tag="VB241_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245794.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHK"
     gene            complement(473..1432)
                     /locus_tag="VB241_002579"
     CDS             complement(473..1432)
                     /locus_tag="VB241_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP1245795.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRIVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHNKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTAHVGERKGGPYIYLNNEELANINPKVMILATNGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLQSRGIMASESMAEDLEKIAEKAK"
     gene            1923..2279
                     /locus_tag="VB241_002580"
     CDS             1923..2279
                     /locus_tag="VB241_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEP1245796.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            complement(2368..>3025)
                     /locus_tag="VB241_002581"
     CDS             complement(2368..>3025)
                     /locus_tag="VB241_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP1245797.1"
                     /translation="INGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEK
                     IVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNV
                     FLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDN
                     KNGYLIENHNINDMADKILKLVNNDVLAEEFGSKARENIIEKYSTESILEKWLNLFNS
                     "
BASE COUNT          942 a          522 c          383 g         1178 t
ORIGIN      
        1 aaggaggatt tacaaatgaa agtcaataaa gccatttatg ttattgttgc attatttctt
       61 ggaggtttag gtattcataa attttatgct gataaatttg gacaaggtct ttttcactta
      121 ttatttttct ggacaggtat ccctaccatc atttcaataa tacatgccat tattgtcatt
      181 tttactaaaa aagcagataa agacggttat attatattcc ctaaaaatta aattgtgaca
      241 atgacgttta aaaagcatat atgaatgggt atatagtaca taagataaag agaaatgagg
      301 tatgtaacga tgcgtcaatt cattaaaaga attgttaaaa caatccttgt cggttatgta
      361 attaaattta ttcgaaataa actttcaggt aaatcatcac atccgacaga taataaacat
      421 aaataaaaaa tacagcgcca ctcttatatg atttacgagt ggcgctgtat ttttattttg
      481 ctttttctgc aattttttct aaatcttctg ccatactttc acttgccata atacctcttg
      541 attgcaacca cttatttcga tcaacatcat atactttatt atctttcaca gcttttaatg
      601 atttccaaac tgcaggatca atgaatttcg ttctattttt atctgttttt ccattcgtag
      661 ctaaaatcat aacttttgga ttgatattgg caagctcttc attatttaaa taaatataag
      721 ggccaccctt gcgttcacca acatgcgcag ttttgtcttt tgtgacttca ggttgaatac
      781 ccattttaat taagaattgt cccataaatg tatcttcatt attaataaac atacctgctc
      841 ttgagatacc aaatgcaaat gcagatttta acgtactctg ttcaattttc tttctaatct
      901 ccgctaatat tttattatgc ttttccagac gcttttcgcc ttctttctct ttgccaactg
      961 ctttagcgac tgttttaaat gcttcaatat ttgcattgta atctcccgta ccgctaacta
     1021 acatgattgt cggcgcaatt ttgctcaatt ctgatttgat tttcttatgt ctgctgacat
     1081 ctgcaataat caaatccggt tttaatttac ttatcacttc catattcggt tgcggtctag
     1141 atccaaccga ttcatatgcc ccaatcttat ctcttactga ctttgttata tttttagtac
     1201 taccatcatc tgcaatacca acaggtttca tatctaatgc tgctaaataa tcagcaaaac
     1261 tatattctaa tacaacaata cgtttcggat ttttcttaat tttttcagtt cctaattcat
     1321 cttttatcgt cgtcatttca gttttctctt cagtatcttt ttgaccacaa cccgctactg
     1381 ctacaactaa gattagcata aacacaacta atttaacgat attcctattc acacatttac
     1441 ctccaaacaa tttttataga gaactatatt attattttat catttaataa acttataata
     1501 tatttaaacg gtcattattt tttcttatca tagttgatta tcaaatcttc gtattataaa
     1561 cagcaaatgg cctctaattt tgatgatgac gcactggtta atattaatca tatcgcttaa
     1621 agtagtattc tgtttgttct cactactact aagcctccac aacagtgttc aagatacgta
     1681 tattttgatg ccataattaa ttcatttacc tataaacttt gcactatgct gtactaatct
     1741 taatttataa atacaaaaag taactgatga cttaaacatc tttctacaac aattgttcaa
     1801 tacaatagaa catttaataa atatttaata attattttta taaataatct ttactttcca
     1861 cttctttaca aatataattt ggactatgtt ttaagaaaaa aatgtatgtt ggaggtgaca
     1921 tgatgaaatt aggtatgcta ttaattagat ggatgctagc aacatcattc ttagtacacg
     1981 gcacattgaa atttgtagat ttagatggaa caattcactt cttagggtct ttaggtttac
     2041 cacctaccat cgcagtattg atgtcaattg gtgaggttgt tggaggttta gcacttatta
     2101 tcggcttttt aacgccttat gttaatgttt tttatatttg tgttatgtta ggctcaatct
     2161 tcacacttaa acttacaaga ggatatgtaa gtggttatga atttgaaatc atacacatcg
     2221 taatgaactt agcttgtatt agtagttaca gctggaagaa atggctacaa ttttattaac
     2281 tattatttga gaggactatc ctctcttttt ttgtatatat catgcgtaaa aatcctaggt
     2341 actcatgaat acctaggatt ttttcattta gctattgaaa agatttaacc atttttctaa
     2401 tattgattcc gttgaatatt tttctataat gttttctctc gctttcgaac caaactcctc
     2461 tgctaaaaca tcattattaa caagctttag tattttatca gccatgtcgt taatattatg
     2521 gttttctatt aaataaccat ttttattgtc ttctataaaa tcacttggtc cgtatttgat
     2581 gtcaaaggca acaacaggcc ttttagaaat cattgcttct atcatactta acccttgacc
     2641 ttcatattga gatgtagaaa caactaattt aaaatcttcc aagcattttt gtggagttgt
     2701 tgtatatcct ttaagaaaaa catttctttc taaattttta tcttcaatca ttttagctat
     2761 tttatctttg acagatcctt ctccatatat atgaaattta acagcattat ctttttttac
     2821 gactaactca gccacttcaa taagcaaatc aattcgcttc gttggtacca ttctagaaat
     2881 atgacctacg attttttcag tttgaaaatg ttttggagca ttatgtatct taacaaaatt
     2941 gctaatagtg aaaatatttt ctacatcaaa ttgattaaga atatctaatc tttgtgcctc
     3001 tgttaataca attacaccgt taatt
//
LOCUS       DATTQF010000198         3011 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.202, whole genome shotgun sequence.
ACCESSION   DATTQF010000198 DATTQF010000000
VERSION     DATTQF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.202"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            381..1124
                     /locus_tag="VB241_002582"
     CDS             381..1124
                     /locus_tag="VB241_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP1245798.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKREDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            1674..1769
                     /locus_tag="VB241_002583"
     CDS             1674..1769
                     /locus_tag="VB241_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEP1245799.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
     gene            complement(1984..2325)
                     /locus_tag="VB241_002584"
     CDS             complement(1984..2325)
                     /locus_tag="VB241_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245800.1"
                     /translation="MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRAPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(2412..>3011)
                     /locus_tag="VB241_002585"
     CDS             complement(2412..>3011)
                     /locus_tag="VB241_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEP1245801.1"
                     /translation="DVIERFVLFNHYRIHKREQYLEISTSSNLKHILNSITNNFTKFE
                     LKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHIDDFKNRLDIPKSYRMTDINKNVFKPI
                     IIELGSIFNNLTINKIKAKKGRKIEWIEFTFDAEKRIHNKRQPQMSKIDKSRQYVRRE
                     KTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQLELNWE"
BASE COUNT         1071 a          443 c          445 g         1052 t
ORIGIN      
        1 gatttttata attttcactg gtaaagaata ctttagggat ttgataaaat ctttctttgt
       61 aattttcttg cactgtaaaa tattgatttt acatgacaaa aaacctcttt ccatatttta
      121 gtttggtaag aggtaataaa acgtttttat ttagctttac atttatttaa tgatttaggt
      181 gcttgcacta agatattatt cattatataa tacaagtatc gaaaattata taaataaaca
      241 gtttctaaac gttttattac caaaccccga ctattaccag tagttggggt ttttgttata
      301 ttaagtttat tatagcactt taaataaact tataaaaggg aaaatatagt tttatcaagg
      361 gtttataaag ggttgatgat atgaaaagtg tgaaaaagtt gagtgaagaa ttaggggtta
      421 gtaagcaaac gatttttaat aatattaagc gattgaatat agagacaata aaacaggaaa
      481 acacttcgtt tattaaagaa gataccgata tagaaaaaat cattcaaaga gtaaatgaaa
      541 ataaaaagaa atatggtttt gaaagtacta ctgaagacaa acaaaaaaaa gggtcggata
      601 atattaattt tgaatcgaaa agcgatgctc aaatagtaga aatccttaaa aatcagattg
      661 atactttaaa taatcaaatt gaaaaacaag aaagtcgtca tgaaacaaca attgaatttt
      721 acagaaaaga gttgcaagaa agatcaaaat tgcttgaaaa tcagcaagtt ctggcattag
      781 agagtaataa aaagattcaa aaattagaaa atcaattaga aaaagaaaaa caccttaatt
      841 atccctttga tacatctact aacactagac aaaacgttga tgcacaagaa aaaacatata
      901 ctactaatcc tgtaaatatt aacagagatc aggaagaaac caaagaaaca gagattcaat
      961 ataaggatat atctggaagt caaagcgacg aaagtacaca aggtgaagaa gcaaaaagag
     1021 aagacgttcc agctaatcta aatgacaatg atagtgatat agaagaaaag agtgaagaaa
     1081 cggaacctaa aaaggggttt tggagtcgtt tgtttggtag ctaaaataat tgattaggca
     1141 ctaaaagtta taaaatttta gtgtctttta tatagtgaga acttctggta atcatcgatt
     1201 atgttaacca aacaaaaagc cataaatgcc gtcatatagg catttatggc tttttacttt
     1261 cattatcttt tatgcatctt ttatacatcg ttgatttgac gcgttttcat tcttttgcat
     1321 tggcaagtat tttgtatctt tattcagtta ctatttcttt tctccaacct aacaaaccat
     1381 ctttaactct aataatatgt ttgccacgcc aggcacttct tacttcgaaa taataccatt
     1441 ccccgttttt gttgaggaaa taaatatctt gataaccttc tttaatgaca tattttgtag
     1501 cttcttatct ttgatcgcca aacattatca atcccaccct tatatttatt tatctttaat
     1561 atataacaac atttattaat attcatgtta tttcatggaa gaagagattt gttataatgt
     1621 gaaggtcgtc tatctctcag acgtcaattc ggcgtagaga ggaggtgcga tttatgaatg
     1681 aaattcttgt tcacatcatg acaacggcaa tcagtggttg tctcgttacg ttatttggtt
     1741 attggctcca taaacgtgat aaaaaataat atagacgatt aaagccaatt caccaaaaaa
     1801 ccccgaacta cggcgaatag ttcggggttt ggtgcgttta caaacgaaat tcttgttact
     1861 tttagtatat atttcatatt gccttttgta aactcttcta ttcttatcaa tacatttcaa
     1921 ggtgtcgtaa aaatactttc ctttttcata ttgcctgatg tgcattaaat tttgatttta
     1981 aaatcaaccc atgaacgaat gaatggccca aactaaaaat ccggcaacag gaaagaaaag
     2041 ccaccaaaaa tcataaagaa aatcaactat ggaaggtgcc ctgttactct ctctataatt
     2101 tccctctaaa ataacttttt taaaacgtgc atctccttta attaaatgct ctaaagagat
     2161 tccaaataaa tcgctcatag caatcaaaat atctaaggaa ggatagtttt tggcactttc
     2221 ccatttagaa acactttgtc tggaaacctt taatatattg gctaattcct cttgagacca
     2281 tctttttaat tctctctgtt cttttatagc ttcagctaat ttcataaaat cactccttaa
     2341 ctcaaactat aattaaaata attgaaaagt cacgatactt ttagttaacc aactcgcaac
     2401 gaaaagttgc gttattccca attcagttca agttgtttta aaaaatcctc tcgttctttc
     2461 tctaattgtg ggtcgtaatc tttttgaggt tgcttttcat atgaccgttc ttctaaccat
     2521 ttaggcgtct tttcacgcct cacatattgt cttgatttat ctatcttgga catttgaggt
     2581 tgtcgtttgt tatgaatgcg tttctcagcg tcaaatgtga actctatcca ttcaatttta
     2641 cgcccttttt tcgctttgat tttattgata gtaagattat taaatataga acctaattca
     2701 ataataatag gtttaaaaac atttttgtta atatcagtca tacgataaga tttaggtata
     2761 tctaaacgat ttttaaaatc atcgatgtga atttttaaat agcctgtatg tttgtattgt
     2821 ttaagtaatc taaacatatt tttagaatat gtagatttaa gcctagtcat ttcttttaat
     2881 tcaaattttg taaaattatt agtaattgaa ttcaatatgt gttttaaatt tgaagaagtt
     2941 gatatttcta gatattgttc tcgcttatgg attctataat gattgaataa aacgaatctt
     3001 tctattacat c
//
LOCUS       DATTQF010000199         3006 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.203, whole genome shotgun sequence.
ACCESSION   DATTQF010000199 DATTQF010000000
VERSION     DATTQF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.203"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(273..1208)
                     /locus_tag="VB241_002586"
     CDS             complement(273..1208)
                     /locus_tag="VB241_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP1245802.1"
                     /translation="MRLQKAPLVTSGLILGLLGLGNLLKDISLVLNAICGIFALFIWV
                     HLLCTILNNFKNVKEQLNTPLVSSVFTTFFMSGFLGTTYLNTYFSDVTIITSLITPLW
                     LLCLMGIMIHMIIFSIKYLKGFSLENVYPSWTVLYIGIAIAGLTAPISGFYLIGKLSV
                     IYGFLATCIVLPIVFKRLKTYPLQTSIKPNTSTICAPFSLVAAAYVITFPKANDLIVI
                     ILLVLAQFFYFYIIFKLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLTYPEIWEW
                     LLFFEITLATLIVLRVFLGYVHFFFKTSPKASYNS"
     gene            2327..2506
                     /locus_tag="VB241_002587"
     CDS             2327..2506
                     /locus_tag="VB241_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245803.1"
                     /translation="MATISITTDYKFTKKSAQKLIDAMEINENNSNVNKTNIKATKIK
                     STCEIENLLKDYRSN"
     gene            2503..2799
                     /locus_tag="VB241_002588"
     CDS             2503..2799
                     /locus_tag="VB241_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245804.1"
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNILNSFETISISGSESA
                     HDVDLFLKNKSIAFDRQNLSRTHLVFSQFKNKQILVGYFTISNKPLVFYKTYVR"
BASE COUNT         1156 a          378 c          493 g          979 t
ORIGIN      
        1 aatggtaatt gaaacctcag atctttagtt taaagctaat tttaataatg caaacattca
       61 agcaagttta agggttggtg gataaagaga aaaaaataat agggtataga attaagtttt
      121 ttaccctata cccagtttta tatgaagcaa gtaaaatcgg cagctgaatg gctggttgat
      181 tttgagcaaa gatttattaa gatatgtctt gtcatatttc tctttatcat tttgtcatta
      241 ctataagata tttttaaaag tgctacatta gattaagagt tatagctagc cttcgggcta
      301 gttttaaaaa agaaatgaac atagcctaaa aagactctta atactattaa agttgctaat
      361 gtaatttcaa aaaataagag ccattcccaa atttctgggt acgttagtac aggtaaacta
      421 ttttttaagg cagttgctga aattactaaa ggaaaagtga aagctgaaaa tactggtgaa
      481 aacggctctt ttagcaactt tggaagttta aatataatat aaaaataaaa aaactgagcc
      541 aataccaaaa gaataataac gattagatca tttgccttag gaaaagttat aacatatgcc
      601 gcagcaacta aagaaaatgg tgcacaaatt gtggaagtgt tcggtttaat agacgtttgc
      661 aatggatacg tttttaatcg tttgaatact attggtaaga caatacatgt tgctaaaaaa
      721 ccatatataa ctgataattt tccaattaaa taaaatccgc tgattggtgc tgttaatcca
      781 gcaatagcaa taccaatata aagcactgtc catgatggat aaacattttc gagcgagaac
      841 ccttttaaat atttaattga aaaaataatc atatgtatca taatccccat aagacataag
      901 agccataagg gtgttattaa gctagtgata atggttacat cactaaaata cgtatttaaa
      961 taagtggttc ccaaaaatcc agacatgaaa aatgttgtga acacagatga aactagaggg
     1021 gtattcaatt gttctttaac atttttaaaa ttattgagaa tagtacataa aaggtgaacc
     1081 caaataaaga gggcaaagat accacaaata gcatttaaaa caagtgatat gtctttcaag
     1141 agattgccca accccaacaa acctaagatc aatcccgatg ttactaaagg tgctttttga
     1201 agtctcatga tttaaaacct tccttttgtg attttattca ctaattataa catgatatct
     1261 tagaactatt aataagcaga atgagttttg tatgtttaat gtggattaat atgatgttgt
     1321 ttcgggaaat atatgtattt atctattttt gatattttat attcagtata atacgtgatt
     1381 acaacgttga atataaaaaa atatgacgat gtttatacgt attaatatta atataatcac
     1441 acgttgcagg catcaaacgc ttatttatta atcaaaaatg ggtggacaaa tttatatagt
     1501 tttatcaatt ttaatatttt acactaactt tattaggtga tataagatgc tgagataagc
     1561 attatattgc aatgaaaaag cattatatgg ataatcatat ctatcattgc aaatatactt
     1621 atagagattt atgtgtgtga taattggtgg tcataaattg gtcataatga aataaaaaaa
     1681 ctaaaaaaat tgaatgcata aagaatacac gatgctgatt taataggatt tttgtatatg
     1741 atttatatct atttcatact gcccttaatg ccaggaatga tgtaaaacag tttctagttt
     1801 tgactaacta gaaaattaca ttatgcacga gtatgattcg ttccaaataa atattagagc
     1861 gtatgaaaat atgtttttaa gacgttttcc attagttacc aaaacttttg gaaggcgtct
     1921 ttattatgtg ctgatgtgtt atttagattc ataatggggg gttttttcaa tgttaaaatc
     1981 atatagatta tatagagaag tttagtatac cttttgagta tataaggagt ttcttctagt
     2041 atgttgtaat gtaattagat ttccggtaat caattcggct ttgcagagga ctcacttgcg
     2101 tattgtaata agaagctgtc tgcattttga aaacacccac actagttaca gcatgggtgt
     2161 ttttatgcta agaaacaaat tggtttaagt gtaaaatcat atacactaaa gaaatttatt
     2221 gaatctattg tcgaaaagat gctttcatga tacattgtaa acaagaagac aacgtagaca
     2281 atgtatactt ggttgtcaaa tgaagtaaaa taaggaagtg agtagtatgg ctacaataag
     2341 tattactact gattataaat tcacaaaaaa atcagcgcaa aaactaatag atgcaatgga
     2401 gattaacgaa aataatagta atgtgaacaa aacaaacata aaagcgacta aaataaaatc
     2461 aacttgtgag attgaaaatc tattgaagga ttatcgaagt aattgactgt aaaagtaata
     2521 tcactttcag aattgttaac aggtgataag caagaggtta agcgaaagat accttcagtt
     2581 ttaaatatac taaattcatt tgagacaata tcaatttcag gaagtgaatc agcacacgat
     2641 gttgatttat ttttgaaaaa taagtctata gcatttgata gacaaaacct gtctagaact
     2701 catttagttt tttcacaatt caaaaacaaa caaatactag ttggctattt tacaattagc
     2761 aataaaccct tagtttttta caaaacgtat gttagataaa atatcaaaca cgttaaagaa
     2821 gaagttatat caaaagggtg aaactcacag tggaaatgac aatttaatca tacaaggata
     2881 cttaattgct caaatagaaa aaaattattc tgaagaagca ttggctacaa aatccataaa
     2941 tgcaaacgat ttattaactt tagcatatgt gaaagtttta gaaggtgcga acatatttgg
     3001 cggttc
//
LOCUS       DATTQF010000200         2927 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.204, whole genome shotgun sequence.
ACCESSION   DATTQF010000200 DATTQF010000000
VERSION     DATTQF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.204"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..520
                     /locus_tag="VB241_002589"
     CDS             <1..520
                     /locus_tag="VB241_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HEP1245805.1"
                     /translation="VSEYESININHRLGKLLDSYDIPDVAKVACLTIDTSMRHLDDIT
                     YNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIVEINELKSSLHHQALNDYE
                     ISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDYYPSYFKKYNQSEVKHYLH
                     DIQKSYLKSRGN"
     gene            523..1248
                     /locus_tag="VB241_002590"
     CDS             523..1248
                     /locus_tag="VB241_002590"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HEP1245806.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGMKALDVCCGTGDWTIALSKAVGSTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEVDFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1250..2209
                     /locus_tag="VB241_002591"
     CDS             1250..2209
                     /locus_tag="VB241_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP1245807.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKEVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2301..2750
                     /gene="ndk"
                     /locus_tag="VB241_002592"
     CDS             2301..2750
                     /gene="ndk"
                     /locus_tag="VB241_002592"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEP1245808.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1086 a          397 c          540 g          904 t
ORIGIN      
        1 tgtatcagaa tatgaatcca ttaatattaa tcatcggtta ggtaagttgc ttgattcata
       61 tgatattcca gatgttgcca aagtagcatg cctcacaatt gacacatcaa tgagacacct
      121 tgatgacatt acatataatc atttgtctaa acattccatt ttgattggag atttaattag
      181 tgcacatttt tatacgctac tagcagaaat caatgattta tcatttcaaa atgaaattag
      241 taaagcaatt gttgaaatta atgaattgaa atcttcatta catcatcaag ctttaaatga
      301 ttatgaaatt tctcaagcaa tagtaaaaat tgaaacactt ttcccatata ttacgttatc
      361 tcatttcggt atcaatattg atgagtcgga aatatataat tatttatttg aagatatgtc
      421 tgattactat ccatcatact tcaagaaata taatcaaagt gaagtaaagc attacttaca
      481 cgatatccaa aaaagttatt taaaaagtag aggtaattaa gcatggctga caataaagca
      541 aataaagagc aagtacatcg cgtttttcaa aatatttcaa aaaaatatga cagattaaat
      601 aatattatta gttttgagca gcataaagta tggagaaaac gtgtcatgaa agacatgggt
      661 gttagaaaag gtatgaaagc attagatgtt tgttgtggta ctggtgattg gacaatagca
      721 ttaagcaaag ctgtaggatc aacaggagaa gttactggca ttgactttag tgagaatatg
      781 ttagaagtag gaaaagaaaa aactgcttca atggaaaatg ttaaacttgt tcatggtgat
      841 gcaatggaat tgccgtttga agacaattct tttgattatg taacaattgg gtttggatta
      901 agaaatgtgc cagactattt agtcgcgtta aaagaaatga atagagtact taaaccaggt
      961 ggtatggtgg tatgtcttga aacgagccaa ccaactttgc cagtctttaa acaaatgtat
     1021 gcattatact ttaaatttgt tatgcctatt tttggaaaat tatttgcaaa atcaaaagaa
     1081 gaatatgaat ggttacagca atctacgttt aattttcctg gaaaagaaga gttgaagcgc
     1141 atgtttgaag aagtggattt cataaatgta agagtacgta gttttacagg gggcgttgct
     1201 gcaatgcacc ttggctataa agaaaaagat aataccaaag gtgattaacg tggcaaagtt
     1261 aaacatgaac aatgaaatta agaaagtgga acaacgactt gaaaaggcaa taaaaagtaa
     1321 agattctgta ttagaacagg catcattaca cttattgtct tctggtggta aaagagtacg
     1381 cccagcattt gtaattctga gtagccaatt tggcaaagat gaacaaacgt ctgaacaaac
     1441 gtatcaagtt gcagtcgcat tagagttaat tcatatggca acacttgttc atgatgacgt
     1501 tattgataaa agcgacaagc gtcgaggcaa gttaaccata tcaaagaaat gggatcagac
     1561 aactgctatt ttaactggga attttttatt ggcattagga cttgaacact taatggcagt
     1621 taaagataat cgtgtacatc aattaatatc tgaatctatc gttgatgttt gtagagggga
     1681 acttttccaa tttcaagacc aatttaacag tcaacagaca attatcaatt atttacgacg
     1741 tatcaatcgc aaaacagcac tgttaattca attatcaact gaagttggtg caattacatc
     1801 tcaatctgat aaagaaactg tacgaaaatt gaaaatgatt ggtcattata taggtatgag
     1861 cttccaaatc attgatgatg tattagactt cacaagtacc gaaaagaaat taggtaagcc
     1921 ggtcggaagt gatttgctta atggtcatat tacgttaccg attttattag aaatgcgtaa
     1981 aaatccagac ttcaaattga aaatcgaaca attacgtcga gatagtgaac gcaaagaatt
     2041 tgaagaatgt atccaaatca ttagaaaatc tgacagcatc gatgaggcta aggaagtaag
     2101 ttcgaagtat ttaagtaaag ctttgaattt gatttctgag ttaccagatg gacatccgaa
     2161 atcactactt ttaagtttga cgaaaaaaat ggggtcaaga aacacatagt atttatgaaa
     2221 agtattgaaa gcgctttacc aacctgttaa tatataatag taatatacat aaacctatta
     2281 aacacagggg gatacataaa gtggaacgta catttttaat gattaaacca gacgcagtac
     2341 aaagaaatct aattggtgaa gtaatttcaa gaattgaacg aaaaggacta aaacttgtcg
     2401 gtggtaaatt aatgcaagta ccaatggaac ttgctgaaac acattatggt gaacaccaag
     2461 gtaaaccatt ttataatgat ttaatttcat ttattacatc agcaccagtg ttcgcaatgg
     2521 tagttgaagg tgaagatgca gttaatgtat ctagacatat tattggcagc accaatcctt
     2581 cagaagcttc accaggatca attagaggtg atttaggttt aactgttggt agaaatatca
     2641 ttcacggttc agattcatta gagtctgctg aacgtgaaat taatttatgg tttaatgaaa
     2701 atgaaattac tagctatgct tcaccacgtg atgcatggtt atatgaataa aatataagct
     2761 ataaaccttt atgattaata aaaggaaaaa gggttttgtt atgtggttag tgatttatga
     2821 ccattcataa caaaacccta tttttatacg gactgattaa ctttataaaa aatgatcagt
     2881 ccattttctt ttatataaag aaatatagct tgtatattat ttattcg
//
LOCUS       DATTQF010000201         2924 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.205, whole genome shotgun sequence.
ACCESSION   DATTQF010000201 DATTQF010000000
VERSION     DATTQF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.205"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            194..559
                     /locus_tag="VB241_002593"
     CDS             194..559
                     /locus_tag="VB241_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEP1245809.1"
                     /translation="MTKDMCEVTYIHEDKVNRAKKDLAKQNPMDVAKVFKALSDDTRV
                     KIAYVLSLEGELCVCDVANIIESSTATASHHLRLLKNLGIAKYRKEGKVVYYSLDDEH
                     VKQLVEKAFLYQREVASIG"
     gene            552..2732
                     /gene="cadA"
                     /locus_tag="VB241_002594"
     CDS             552..2732
                     /gene="cadA"
                     /locus_tag="VB241_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase CadA"
                     /protein_id="HEP1245810.1"
                     /translation="MDSSAKTLTEDKQVYRVEGFSCANCAGKFEKNVKQLAGVQDAKV
                     NFGASKIDVYGNASVQELEKAGAFENLKVFPEKLANSSMQAVKEDTKAPKEEKIPFYK
                     KHSTLLFATLLIAFGYLSHFVNGEDNLVTSMLFVGSIVIGGYSLFKVGFQNLIRFDFD
                     MKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEAL
                     VMRNGQEIMIHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGESVPVAKTV
                     DDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYTP
                     IIMVIAALVAVVPPLFFGGSWDTWVYQGLAVLVVGCPCALVISTPISIVSAIGNAAKK
                     GVLIKGGVYLEELGAIKAIAFDKTGTLTKGVPVVTDFKVLNDQVEEKELFSIITALEY
                     RSQHPLASAIMKKVEQDNITYSDVRVEDFTSITGRGIQGNIDGTTYYIGSPRLFKELN
                     VSDFSLEFENKVKVLQNQGKTAMIIGTDQTILGVIAVADEVRETSKNVIQKLHQLGIK
                     QTIMLTGDNQGTAEAIGAHVGVSDIQSELMPQDKLDYIKKMKAEHGNVAMIGDGVNDA
                     PALAASTVGIAMGGAGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAI
                     GIKIIALLLVIPGWLTLWIAILSDMGATILVALNSLRLMRVKDK"
BASE COUNT          940 a          467 c          651 g          866 t
ORIGIN      
        1 aactaacggg gcagttatgt ttaagatact ttaaaatttg gttaaaatct ccgaaaggat
       61 tggtttctaa cattagaaat caatcctttt taatattaat tatatatcat tcaaacaatc
      121 atttgactat ttgtttatgg tgggatatat tataatcatc aaacaaacag tcatttgaat
      181 aaggaggata attatgacaa aagatatgtg tgaagttacc tatattcatg aagataaggt
      241 aaacagggct aaaaaagacc ttgctaaaca gaatcctatg gatgtagcga aagtttttaa
      301 ggctctatca gatgatacaa gagttaaaat tgcttatgtt ttgtctttag agggagagtt
      361 atgtgtttgt gatgtagcta atatcattga atcttcaacg gctacggcat cccatcattt
      421 aagattattg aaaaatttag gtatagcaaa ataccgtaaa gaaggaaaag tagtctatta
      481 ttcactagat gatgagcatg ttaaacagct tgtagaaaaa gctttcttgt atcaaaggga
      541 ggttgctagt attggatagt tcagcaaaaa cattaacaga agataaacag gtttaccgtg
      601 tggagggttt ctcgtgtgcg aattgtgctg ggaagtttga aaaaaatgta aaacaactag
      661 ctggagttca ggatgcaaaa gtgaactttg gcgcttccaa aattgatgta tatggaaatg
      721 catcggttca agagcttgaa aaagcaggtg ctttcgagaa tcttaaggta tttcccgaaa
      781 aactagcgaa ttcatcgatg caagcggtca aagaagacac taaggctcct aaagaagaga
      841 aaataccgtt ttataaaaaa cacagcacat tgctgtttgc cacattactg attgcttttg
      901 gttacctttc tcactttgta aatggagaag ataaccttgt aacttccatg ttatttgtag
      961 gttcgattgt aattggcgga tattcactat ttaaagttgg ttttcaaaat ttgatacgct
     1021 ttgatttcga catgaaaacc ctgatgaccg tagcagttat tggagctgcc atcattggtg
     1081 aatgggcaga ggcatccatt gttgtcgttc tctttgcaat cagtgaagca cttgaacgtt
     1141 tttctatgga tagagcaaga cagtccattc gttcattgat ggatattgcc ccaaaagaag
     1201 cacttgttat gcggaatggt caggaaataa tgatccatgt ggacgatatt gccgtgggtg
     1261 atattatgat cgtcaaacca ggggagaaaa ttgccatgga tgggatcatt ataaatggtg
     1321 tgtcggctgt caaccaggct gctataacag gagaatctgt ccctgttgcc aaaacggtag
     1381 atgatgaagt atttgcaggt acgcttaacg aagagggact acttgaagta aaaatcacca
     1441 aatacgtaga ggatacaact atctccaaga ttattcatct ggttgaggaa gcacaagggg
     1501 agcgcgctcc agcgcaagca ttcgtagata aatttgcgaa atattatacg ccgatcatta
     1561 tggttattgc ggcgctcgtt gcagtcgttc cacctttatt ctttggtgga agttgggata
     1621 cttgggttta tcaaggatta gcggtacttg tagttggatg tccgtgtgca ttagttattt
     1681 ctactccaat ctcgattgtc tcggcaattg gaaatgcagc taaaaaaggt gtgttgatta
     1741 aaggcggtgt ctatctagag gaattaggag ccattaaggc aatcgcattt gataaaacag
     1801 gaacactgac aaaaggtgta ccagtggtaa cagattttaa agtgttaaat gatcaagtgg
     1861 aagaaaaaga gctgttttcc attattacag ctttagaata tcgatcacaa catccacttg
     1921 cttcagcaat aatgaagaaa gtagagcaag ataatattac ttattccgat gttagagtgg
     1981 aggacttcac ttctattaca ggtcggggca ttcaagggaa tatagatgga acaacctatt
     2041 acattggcag tccaaggctt tttaaagaat taaatgtttc cgattttagc cttgagtttg
     2101 aaaataaagt gaaagtttta caaaaccaag ggaaaacggc catgattatt ggaacggacc
     2161 aaacaatcct cggcgtgatt gctgtagcag atgaggtccg cgaaacaagt aaaaatgtga
     2221 ttcaaaaact tcatcagtta ggaatcaagc aaacaattat gctgacaggt gataatcaag
     2281 gtaccgcaga agcaatcggt gctcatgtag gcgtttctga tattcagtcc gaattgatgc
     2341 cacaggataa gttggactat attaaaaaaa tgaaagccga gcatggtaat gtagctatga
     2401 ttggcgatgg cgtcaatgat gctcctgcac ttgctgcatc cactgttggc attgcaatgg
     2461 gcggtgctgg aacagatact gccatcgaga cagctgatat tgcattaatg ggagatgatt
     2521 taagtaagct tccatttgca gtaagactta gcaggaaaac gctaaatatc atcaaagcga
     2581 acatcacgtt tgccatcgga attaagataa ttgccttact attggttatt ccaggctggc
     2641 taaccctttg gattgcgatt ctttccgata tgggagccac tattttggta gcattaaata
     2701 gtttgcgact gatgagagtg aaggataaat aggtactata tgtgtgcaat ttacggagag
     2761 gctgggacat aaatccctaa aaagcagcag taagataatt ttcaattaga aaatatctta
     2821 ctgctgttct ctatttatac aatacttcgt attgaatggc ttcgctttgc ccgtctggca
     2881 cattactgta aaattctata aatagaattt ttgatgacgg atcc
//
LOCUS       DATTQF010000202         2881 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.206, whole genome shotgun sequence.
ACCESSION   DATTQF010000202 DATTQF010000000
VERSION     DATTQF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.206"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            237..2342
                     /gene="rsp"
                     /locus_tag="VB241_002595"
     CDS             237..2342
                     /gene="rsp"
                     /locus_tag="VB241_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HEP1245811.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPTSDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            2413..2835
                     /locus_tag="VB241_002596"
     CDS             2413..2835
                     /locus_tag="VB241_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP1245812.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMIFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
BASE COUNT         1070 a          445 c          385 g          981 t
ORIGIN      
        1 actctatcga aaatttccaa attttgctta ctatctttct aattttactt ttatattgtg
       61 cacgatggat aaaaacaagc gttttcctag gagttttctt taatttcttt tatttttcac
      121 tattaccttt tcaaaaatat tcaactataa taggtatact atccatgatt ttaaaaattt
      181 ccatgtttta catttaaatg atagatttca atatatatta ggcaggagag agaaatatga
      241 catgccaact taaaatacat catacattat cgacaatacc aagtcgtaat atcaataata
      301 ttatggtgct attttcactt acaagtaaac tcaatattac gattaatggt gaaaccaaag
      361 acgtaagtaa ttatattatc ttaattaatc atggtgatat ttataatatt aaccacggcg
      421 aaaatataat cgaattaatg atacccgttt tttattttta tcaacaagat gatgatttct
      481 ttaatggtta tttagatcgt catttattac aatcaagtaa ttatattaag tcattaattg
      541 ccgatttaat cagtacacca acaagttctt ctttaatggg aaaaaatata ggtcaaagta
      601 ttatagacac tttacttaaa gaagctttta taagaataga tcatgaatat ttaccaaata
      661 ttgctttaag caatccggtg tttatagact gtgtaaacta tattcatgac aatattgatg
      721 cgcacttgtc tttaaaagat atagcgatgc attgcaacat ctctgaatca tattgttcta
      781 atctttttgt tcgatattta agtatgaatt ttaaagatta ttttacaagt attaaacttg
      841 ttaacgccat caatttatta ctttctacaa aacattctat tacaactgtt tctgaattgg
      901 ccggttttaa cagtcataca aattttgcaa atcaatttaa gaattacttg cattttagtc
      961 ctaaacagtt ccgttcactc gtttccaaga ttactgaacc accacaaata cacttccaac
     1021 aagataacgt atcacaattc actgagttaa tttcaacaat tgatttaaca gctcagctgg
     1081 ctacaaatac aacagatatt catatagatg attttaatcc gaaagatcga agtcagcgtg
     1141 ccaaagtatt tgttcgattt agtaatttca atgaactatt tcaatttatt ttcaatgaat
     1201 attacgatat taactttgag cacctaccaa aacctgtcgt tttcattgac gatattcacg
     1261 atattgagat tagccaaacg aattacaacc tgttaaatag atgttttgaa aagcttttcg
     1321 aaaaaaatat aggtttagca atcgctatta aatcaacaca acaattcgaa acgatgaagc
     1381 aattaatact aacattttta caaggcaatc aagactataa gacaagcaag aaattagtta
     1441 aatttatgct cgtattttgc tctaactcta tgacagcaga agaaattcat ttatgtcatc
     1501 ttaaaattaa aaacaaaaat aaagaaatta aatatagtgt gacagtcgat ggctttttag
     1561 aaacttattc aaccgtggaa caagtttatg atgtcatgca gcgtcttaaa ttccattatt
     1621 actttattga tattgaaaat tcaaaaactg caacacatct tatcactaaa aaccaacact
     1681 atcatcaaac tgacactcat tttgaacagt ataagaaatt tattttagat tcaggtatat
     1741 catcaacaca atttgtatat aataacctgt ctgtaagtgg atttaaatat actaatgatg
     1801 gtaagaatcc aattcaatta tctgacatag tgtatcactt aatcgcatta ttgcgatatg
     1861 gcggtggcat tagctatcaa ttattagatg accattcaaa ttatatttcc ttgtacaaca
     1921 aatatggtag ccccctacca ttaatgcatt tatataaaat gtttagacct tttgttaatg
     1981 aagacattga aattacaaat aattatgtat tgagtcgtaa agataataac taccatttct
     2041 tattattcaa taaaattaat gaccgatata tgtcagacgt aaaacaagac ttcattttcc
     2101 ataatgaatt acctcaagac tctttgatga ttattaaaac attgaatcat gaacatggtt
     2161 caattcaaca tttgcttcca acaagcgatc aacttgtcta tatagaaaaa gaaattctag
     2221 atgaattaga caaaacgaat taccctaaaa cggagcttgc agttcaagaa gaaactggtc
     2281 gtacatttga actcaagtta aatcacgacg aggttaaata tatttgcttt aaaccaagct
     2341 aaatactaac agtcctcttg tgtttagttt cttacgttaa aggctattta tatcataagg
     2401 agatgatata ggatgagtaa ctcacaagca attcaagcaa ttgaaaacgt gctagcaacg
     2461 tcaaaagttg gtgtattatc aactgcatat aataacaaac ctaatagtag atatatgatc
     2521 ttttataatg atggtcttac tttatatact aaaacgaata tccattctgc taaggtcaaa
     2581 gaaattaaag ataatccagc agcatatgtt ttattaggct ataacgacac aactaatcgt
     2641 agtttcgttg aaatggaagc gacaatcgaa atcgttacag aacaagaagt gattgattgg
     2701 ctatgggaaa cacaagacaa aagctttttc agttcaaaag aagacccaga gttatgtgtt
     2761 ttaagagtag ttccacaatc cattaagcta atgaatgata aatcattaga tacacctatt
     2821 aaaattgatt tataatatgt aagtttttag ttttaaaatt agtcagcaat tgaacatgtt
     2881 a
//
LOCUS       DATTQF010000203         2875 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.207, whole genome shotgun sequence.
ACCESSION   DATTQF010000203 DATTQF010000000
VERSION     DATTQF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.207"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            42..692
                     /locus_tag="VB241_002597"
     CDS             42..692
                     /locus_tag="VB241_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEP1245813.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSAKNSERRGMLKGCGGCLISF
                     IILIILLSACSMMFSNNDNSTSNQSSKTQLTQKDEDKSENMPEEKSESETDKDLQSTE
                     EVPANENTENNQHEIDEITTTDQSDDEINTPNVAEEESQDDLKDDLKEKQQPSDHHQS
                     TQPKTSPSTETNKQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            773..1768
                     /locus_tag="VB241_002598"
     CDS             773..1768
                     /locus_tag="VB241_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEP1245814.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDNSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1844..2470
                     /locus_tag="VB241_002599"
     CDS             1844..2470
                     /locus_tag="VB241_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245815.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMNDQAEMDNESEDI
                     QHHDIDNNKAIQNDLPDQKVDDKNDKNAVNKEEKHDNRENNSAETKVK"
     gene            2511..2855
                     /locus_tag="VB241_002600"
     CDS             2511..2855
                     /locus_tag="VB241_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HEP1245816.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSTLGVKPDIIDLIHKGTELEDFSAFNLSIDDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIMQEK"
BASE COUNT         1183 a          478 c          481 g          733 t
ORIGIN      
        1 taactccctc ttaaatgcat ccaggtttta tgtagtaaat catgaaaata acatataaat
       61 atagaggaga tttacctttg aatacagaga acaacaagaa tcaaaaccaa tctgctaaaa
      121 attctgaaag acgtggcatg ttaaaaggat gcggcggttg ccttatttct tttattatat
      181 taattatctt attatcagcc tgttcaatga tgtttagtaa taatgacaat tccactagta
      241 atcaatcatc aaaaacgcaa ttaactcaaa aagacgaaga taaaagtgaa aatatgcctg
      301 aagaaaaatc agaatcagaa acagataagg atttacaatc aaccgaagaa gtacccgcaa
      361 atgaaaatac tgaaaataat caacatgaaa ttgatgaaat aacaacaaca gatcaatcag
      421 atgatgaaat taacacacca aacgttgcag aagaagaatc acaagatgac ttgaaagatg
      481 atttaaaaga aaagcaacaa ccaagtgacc atcatcaatc cacgcaacct aagacttcac
      541 catcaactga aacaaacaag caacaatcat ttgctaattg taagcaactt agacaagtat
      601 atccgaatgg tgtcactgcc gatcatccag catatcgacc acatttagat agagataaag
      661 ataaacgtgc atgtgaacct gataaatatt aaacaacaag cgaattgaat tcaaattgta
      721 tttagcttta tgcactaatc acatagtaaa taatgaggga gattttttag gcatgagcaa
      781 tcaattcaaa agcgaagaag agcgaagaca atgggaacaa ttccaagctt tccaaaatca
      841 acaaaaccaa cagaaccagc aatacggaca aaagaaatct aaaaaaggat ggttctgggg
      901 ctgtggtggt tgtctagtat tattcatttt aattatcatc ggtatttcag cttgtacagc
      961 tggtattaca ggtaaccttg gcggaaatag ttctaaagaa acgaacaaaa cccataaaat
     1021 cggtgaaact gttaaaaatg gcgaccttga agtcactgta aattcagtgg aaactatgaa
     1081 atctgtagga ccatctcttg caccaacaaa cgctaaaggt atatttgtcg ttgctgatgt
     1141 gacgattaaa aacaaaggta aagaagcgtt aacaattgat agttcaatgt ttaagctaaa
     1201 atccggtgat aaaacatttg aagcagataa tacaggttca atgtctgcta atcaaagtga
     1261 caatggtagt atagaaaatt catttttctt acagcgtata aatccagata gcactgctca
     1321 aggtaaaatt gttttcgatg tgtcagaaaa catagccaac gcaaaagata aaaaattaga
     1381 agttatttct agtttattta gcgtcaagaa gattacattt gatttatccg atgctaaaaa
     1441 aacatcaaaa gctaaaaaag acaagcaaga tacagaagta gctgttgcga gttcaaatag
     1501 cgataatgta agttatgaag cttcggctac tacacctgct acaacttcta gtgcggatac
     1561 tgattctgaa gatagcgaaa agtctagtaa agatgaggat aagcagaatg cgtctaaaag
     1621 tgataattct agtgtagaaa aaagtgaatc taatgaggaa actgctcctg tagagcccat
     1681 gccccatagc aaacctacca ctagtgaagc acctcctagc caaaatattc acaatgaaga
     1741 tagcatgtac gacgcttcaa cagaataaaa tttcagggta gctcggctac ccttctttta
     1801 cggaaaaatt aattatacat aatcaaatca aggagataaa aaaatgaaat tcaaagctat
     1861 cgttgcaatc acattatcat tgtcactatt aaccgcctgt ggtgctaatc aacataaaga
     1921 aaatagtagt aaatcaaatg acactaataa aaagacgcaa caaactgata acactacaca
     1981 gtcaaataca gaaaagcaaa tgactccaca agaagccgaa gatatcgttc gaaacgatta
     2041 caaagcaaga ggtgctaacg aaaatcaaac attaaattat aaaacaaatc ttgaacgaag
     2101 taatgaacat gaatattatg ttgaacatct agtccgcgat gcagttggca caccattaaa
     2161 acgttgcgct attgttaatc gacacaatgg tacgattatt aatatttttg atgatatgtc
     2221 agaaaaagat aaagaagaat ttgaagcatt taaaaagaga agccctaaat acaacccagg
     2281 tatgaatgat caagctgaaa tggataatga gtcggaagac attcaacatc atgatattga
     2341 caataacaaa gccattcaaa atgacttacc agatcaaaaa gtcgatgata aaaacgataa
     2401 aaatgctgtt aataaagaag aaaaacacga taaccgtgaa aataattcag cagaaactaa
     2461 agttaaataa gggtatactt tgattaatcg taatttttag gagaaccgct atgataacat
     2521 ttgaaaatat acaacaactt gaaaaataca ccttaatgac tatgcatggt ctttttaatc
     2581 aacttaaact cggtattatt tcaatcgaca atgcagagca tacgctcttt acaccttata
     2641 tgatggaaac actctctacc ctaggtgtga aaccagacat tatcgattta attcacaaag
     2701 ggacagagtt agaagacttt tcagcattta atttatcaat tgatgataca gttacagtct
     2761 gtttacaaag aactgaagaa ctattaaaac aatacaaaaa tgtagaattc aatgacaaaa
     2821 tattaatcaa ttggcgcatt atgcaagaga aatagacata taaaagtaga accta
//
LOCUS       DATTQF010000204         2821 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.208, whole genome shotgun sequence.
ACCESSION   DATTQF010000204 DATTQF010000000
VERSION     DATTQF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.208"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            72..>2821
                     /gene="sdrC"
                     /locus_tag="VB241_002601"
     CDS             72..>2821
                     /gene="sdrC"
                     /locus_tag="VB241_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HEP1245817.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNEATAPSENKTTEKVDSRQQNNVEQSTTSNQPKVNESDNT
                     SVKETTEEPQNTTSTQPTKKNNDATANKDNLAAQNISTQANDVSATPKTTTIKPRTLN
                     RMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNSTTGKTEFWATSSDVLKLKANYT
                     IDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTYTFT
                     NYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDAYSEEIIVDYGNKKAQPL
                     ISSTNYINNEDLSRNMTVYVNQPKNTYTKETFVSTLTGYKFNPDAKNFKIYEVTDQNQ
                     FVDSFTPDTSKLIDVTDKFKITYSNDNKTATVDLMNGQTNSNKQYIIQQVAYPDNTST
                     DNGKIDYTLDTDKTKYSWSNSYSSVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANE
                     KGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLGNGTYSVEFSTLAGYTPTTVNAGT
                     DDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKG
                     VKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLKQTGTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEETSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSD
                     SDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSGKHTPVKPMSATKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLL
                     LFG"
BASE COUNT         1127 a          463 c          516 g          715 t
ORIGIN      
        1 gaaaggcaga aatttttata tgcaaaatat tatatttatc aaattttgat attttaaagg
       61 agtattatta aatgaataat aaaaagacag caacaaatag aaaaggcatg ataccaaatc
      121 gattaaacaa attttcgata agaaagtatt ctgtaggtac tgcttcaatt ttagtgggga
      181 caacattgat ttttgggtta agtggtcatg aagctaaagc ggcagaacat acgaatggag
      241 aattaaatca atcaaaaaat gaagcgacag ctccaagtga gaataaaaca actgaaaaag
      301 ttgatagtcg tcaacaaaat aatgttgaac aaagtacaac gtcaaaccaa ccaaaagtaa
      361 atgagtctga taatacttca gttaaagaaa ctactgaaga acctcaaaat acaacttcta
      421 cacaaccaac taaaaaaaat aacgatgcga cagcaaataa agataattta gcggctcaaa
      481 atatttcaac gcaggctaat gatgtttcag ctactcctaa aacaacgact attaaaccaa
      541 gaacattaaa ccgtatggct gtgaacacag tagcagcacc acaacaaggt acaaatgtaa
      601 atgataaagt tcatttttca aatattgata ttgctattga taaaggacat gtcaattcta
      661 caacaggtaa aactgaattt tgggcgactt ctagtgatgt tttaaaatta aaagcgaatt
      721 acacaatcga tgattctgtt aaagagggcg atacatttac atttaaatat ggacagtatt
      781 tccgtccggg ttctgtaaga ttaccttcac aaactcaaaa tttatataat gcccaaggta
      841 atattattgc aaaaggtatt tatgatagta caacaaacac aacaacatat acttttacga
      901 actatgtgga tcaatataca aatgttagtg gtagctttga acaagttgca tttgcgaaac
      961 gtgaaaatgc aacaactgat aaaactgctt ataaaatgga agtaacttta ggtaatgatg
     1021 cgtatagcga agaaatcatc gtcgattatg gtaataaaaa agcacaaccg cttatttcaa
     1081 gtacaaacta tatcaataat gaagatttat cacgaaatat gactgtttat gtaaatcaac
     1141 ctaaaaatac atacacaaaa gaaacatttg ttagtacttt aacaggttat aaatttaatc
     1201 cagatgcaaa aaactttaaa atttatgaag tgacagatca aaatcaattt gttgatagct
     1261 ttacacctga tacttcaaaa cttatagatg ttactgataa attcaaaatc acatacagca
     1321 atgataataa gactgcaaca gttgatttaa tgaatggtca aacaaatagt aataaacaat
     1381 atattattca acaagttgct tatccagata atacatcaac agataatgga aaaattgatt
     1441 atacattaga tacagacaaa actaaataca gttggtcgaa cagttattca agtgtaaacg
     1501 gctcatcaac tgctaatggc gaccaaaaga aatataatct aggtgactat gtatgggaag
     1561 atacaaataa agatggtaaa caagatgcca atgaaaaagg gattaaaggt gtttatgtca
     1621 ttcttaaaga tagtaacggt aaagaattag atcgtactac tactgatgaa aatggtaaat
     1681 atcagtttac aggattggga aatgggactt acagtgtaga attttcaacg ctagcagggt
     1741 acacaccaac tactgtaaat gcaggaactg atgatgctgt agattctgat ggactaacta
     1801 caacaggtgt cattaaagac gctgataaca tgacattaga cagtggtttc tataaaacgc
     1861 caaaatacag tttaggtgat tatgtttggt acgacagtaa taaagatggc aaacaagatt
     1921 caactgaaaa aggaattaaa ggtgttaaag ttactttgca aaatgaaaaa ggcgaagtca
     1981 ttggtacaac tgaaacagat gaaaatggta aataccgctt tgataattta gatagcggta
     2041 aatacaaagt tatttttgaa aagcctgctg gcttaaaaca aacaggtaca aatacaactg
     2101 aagatgataa agatgcagat ggtggcgaag ttgacgtaac aattacggat catgatgatt
     2161 tcacacttga taacggatac ttcgaagaag aaacatcaga cagtgactca gacagtgatt
     2221 cagattcaga tagcgattca gactcagata gcgattcaga ctcagacagt gaatcagact
     2281 cagacagcga ctcagattca gacagcgact cagactcaga cagcgattca gactcagata
     2341 gcgactcaga ttcagaaagc gactcagact cagacagcga ttcagactca gatagcgact
     2401 cagattcaga cagcgactca gattcagaaa gcgactcaga ctcagacagc gactcagatt
     2461 cagacagcga ctcagactca gatagtgatt cagattcaga cagcgactca gattcagaca
     2521 gcgactcaga ctcagaaagc gactcagatt cagacagcga ttcagaatcg gatagcgact
     2581 cagacagcga ctcagactca gacagcgact cagactcaga tagcgactca gattcagaca
     2641 gtgactcaga tagcgactca gattcaggaa aacatacacc tgtgaaacca atgagtgcta
     2701 ctaaagatca tcacaataaa gcaaaagcat taccagaaac aggtagtgaa aataacggct
     2761 caaataacgc aacgttattt ggtggattat tcgcagcatt aggttcatta ttgttattcg
     2821 g
//
LOCUS       DATTQF010000205         2770 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.209, whole genome shotgun sequence.
ACCESSION   DATTQF010000205 DATTQF010000000
VERSION     DATTQF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.209"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..115)
                     /locus_tag="VB241_002602"
     CDS             complement(<1..115)
                     /locus_tag="VB241_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEP1245818.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFV"
     gene            339..1268
                     /locus_tag="VB241_002603"
     CDS             339..1268
                     /locus_tag="VB241_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEP1245819.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAGYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQRSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGINLTSSRFIKWLNDMNVIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQSINNKQ"
     gene            complement(1335..1769)
                     /locus_tag="VB241_002604"
     CDS             complement(1335..1769)
                     /locus_tag="VB241_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEP1245820.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(1785..>2770)
                     /locus_tag="VB241_002605"
     CDS             complement(1785..>2770)
                     /locus_tag="VB241_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEP1245821.1"
                     /translation="IYLFYSPRLKFDVLENPNKGNKVNRSEQVNKSNNHAENPKPKEG
                     VGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVYATGE
                     NVNVSPLKIGQHSAEIFNHTSINPEPSFTVDGKKYEFELSDEDLKTQTLIKYGNIYAQ
                     VYSDQQSKKVLSVRFLTKEMLADIEPYRLNSNSTSEEHNKRPVEQNPNQLISLYEVTN
                     EMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTEDALRGQLDKNHVTYKTTA
                     QNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVMRDYYSLIFLEK"
BASE COUNT          901 a          485 c          418 g          966 t
ORIGIN      
        1 ttacaaattt acgatctcta tttgaatgaa cgatatgtcc atattttcgg atttgtcgtt
       61 catgtttcat atgttttaaa taaactacaa tactagttct tgggattaaa ttcatttaaa
      121 cctcactcca tttattttca gcattaatca tatcatgtct ttattaattt taaaatatta
      181 agctacctta attatgtctt atgtaattgc acgataaatg tatttagatt atattaattc
      241 aattctcaag agcaacacta tatgattaac cgcacatttt gctgtagtca gaactatgaa
      301 tttgttaagc tataaaagta aacgaggagg aattttttat gaaaaaagta aacagaaaca
      361 ttttaaatgc ggtactagtc ggtgctggat ttgtaagtag tttgttaatg ataaataaaa
      421 ataaagtgat tactaaaaag caaaccatcc ctgctttttt caaaggcaat gcaccatata
      481 tatttgcgca tcgcggtggt atggctttaa gaccagagca aacgcaactt gcatttgact
      541 atgcaaaaca attaggcgtt gatggttttg aaacagatgt gagactcaca aaagatcaac
      601 agcttatcgt ttttcatgac gcaacagtcg atagaacgac gaacggctct ggaaaagtca
      661 gtgcccatac attagcagaa ttaaaaaaat tagacgcagg ttatcacttc aaagatatca
      721 atggactaac accatatcgt ggtcatgcac acacagctat actaaccttc gatgagttgc
      781 tgaagcaata tcctgatatg tatataaacg tagacttaaa ggatgctcct gaatcctatg
      841 aaggctcaat cgcaccacaa attatgtttg atacaattgc agagaatcaa gcatttgacc
      901 gtgttcttgt gacaagtttt tataaagaac aaatcgtacg ttttaataaa attgcacaaa
      961 gatctgttgc aattggtgct agccaacagg aagttacaga ggcattttta aaatatcatt
     1021 tattaggtgg taggtactac caaccattag cacaaacgtt ccaaatgcct actcatttta
     1081 aaggtattaa tttgacttca tctcgtttta tcaaatggtt aaatgatatg aatgtcattc
     1141 caggctatta tggcgtaaat agtatagatc tgatgaatga tttataccaa aaaggtgcac
     1201 acacgattgt gacagatcga ccagatttag cacaacaatt caaacaatca ataaacaaca
     1261 aacaataaac aaaaacatcg agtcagatgc tagtaaatgg tttcaataac catctttttc
     1321 tgactcgatg ttttttataa tgaacaatta caggatccgc ccgtcgcaca accactatgt
     1381 gatgtttgaa agaatggatt gcctgtttct attttgacat gttctgaaac agcgtacgca
     1441 attttcgtaa caacttcatc aattaaatgt tgaagtgcca tttccctagc tttgtaatgc
     1501 atcacaacgt caagcatctc atatgctctt ttgcgttgtc ttgtctctaa catgactttc
     1561 ttataatcag gatgatattt cccgaaacgc attacttcat catatttctc ttttgatttt
     1621 aaaaatgatt gataaagtaa atgggcttcg tcattgtttt cgagcgcctg tttagcttgc
     1681 tcaaatgatg cataaatatc tgatgcaact atcatatcag ccagttcatc aatatcatct
     1741 aatatcgcta gtgaagcttc attaatcata aatacccctc attgttattt ttctaagaaa
     1801 attagtgaat aataatctct cattacatct ccacccatct catcgtattt tgaatttaat
     1861 agacgagatc gatgtatatc tgaattcatc caactatgga ttaatgttgg tacatcatta
     1921 aacgcataac caacattttg agcagttgtt ttataagtaa catgattttt atctaattgc
     1981 cctcttaatg cgtcctctgt aaattcaaca ctatcagaac cattagaggt cgcttcatat
     2041 aagttattag atgcaatatg tgctaaatcg ctattgattt tcaatggttt taatcctttt
     2101 aattttctca tttcattcgt tacttcataa agagaaatta attgatttgg attttgctca
     2161 actggacgct tattatgctc ttctgacgta gaattagaat ttaatcgata aggttcaata
     2221 tctgctaaca tttcttttgt taaaaatcgt acacttagca cctttttcga ttgttgatca
     2281 gaatacactt gtgcatatat gttgccatat ttaatcaatg tttgtgtttt taaatcttca
     2341 tctgaaagtt caaattcata ttttttacca tcaactgtaa aggacggttc tggattaata
     2401 cttgtatgat taaaaatttc tgcagaatgt tgtcctattt ttaacggact aacattgaca
     2461 ttctcacctg tagcatacac tgaaacgatt tcttcacgtt tagttgaaac aatgtaataa
     2521 ctgtttttgt ctttaaacac ataatttttg taaccatctc taaaagggta gacacgatct
     2581 gcttgtccaa atttagaagt aagcacttta atgtctttac ctacccatgt accaacacct
     2641 tctttaggct ttggattttc agcatggtta tttgatttat tcacctgttc agatctattt
     2701 actttattac ctttattcgg gttctctaat acatcaaatt ttaatctcgg tgaataaaaa
     2761 agatatatta
//
LOCUS       DATTQF010000206         2720 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.210, whole genome shotgun sequence.
ACCESSION   DATTQF010000206 DATTQF010000000
VERSION     DATTQF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.210"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            390..2485
                     /locus_tag="VB241_002606"
                     /pseudo
     CDS             390..2485
                     /locus_tag="VB241_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
BASE COUNT         1063 a          383 c          448 g          826 t
ORIGIN      
        1 gcttgacata gtattaaata cataaagtcg cgctgatttg cttttcgtat tatgtataaa
       61 atagcaatct tattctaaat taatatcttt ttaagttcac ttatcatttt attacttaaa
      121 tcaaaatttg tctcttttct aacattttac tttacatcaa ttttaattac tttaaatatt
      181 gtataaatta attatttaaa atgctataat cgataagatt attaatattt aattttccaa
      241 ttttaatttc tttacatttt tagatattaa ataaatctca tggtgactga tggtttccat
      301 gcatcaacga atttcccaat cctttggaaa gttgaatcat taattaatcc taatatcaat
      361 tgcacacacg caaaaaagga gacatgtcaa tgacaaataa aatgaagaaa tggcaaaaat
      421 tatccaccat tacgttatta atgaccggag tgattgcttt aaataatggt gaatttagaa
      481 atgttgataa acatcaaatc gctgtggctg atacgaatgt tcaaacgcca gattatgaaa
      541 aattgaagaa gacgtggctc gacgttaact acggttatga tcagtatgat gagaataatc
      601 aagatatgaa gaagaagttt gatgctaaag aaaaagaagc caagaagtta cttgatgaca
      661 tgaaaactga tacgaataga acatatttgt ggtcaggagc tgaaaacctt gaaactaatt
      721 cttctcacat gacaaaaacc tatcgtaata tcgagaaaat cgcagaatca atgcaacata
      781 agaatacggt attaaaaaca gttgaaaaca agttgaaaat aaaagaagcc ctagattgga
      841 tgcacaaaaa tgtttatggc aagaatcctt ctcaaaaagt cgaggattta actaaaaatc
      901 gtaaggggca aactacaccc aagaataact cattgaattg gtgggattat gaaattggta
      961 cgccaagagc attaacaaat acactacttc taatggatga tatgctcact aaagatgaaa
     1021 tgaaaaatta ttcaaaacct attagtacat atgcaccatc cagtgacaaa attttatctt
     1081 ctgttggtga atcagaagat gctaaaggtg gaaatttagt ggacatttct aaagtaaaac
     1141 ttttagaaag tgttattgaa gaagatgtag atatgttgaa aaagtctata gattctttta
     1201 ataaagtgtt cacttatgtt caagattctg ccactggtaa aggtcgcaat ggattctata
     1261 aagatggctc ttacattgat catcaagatg tcccttacac tggtgcttat ggtgttgtac
     1321 tattagaggg tatttctcaa atgatgccga tgataaaaga atctcctttt aaaactacac
     1381 aagataatgc tacattaagc aattggattg acgaagggtt tatgccatta atctataaag
     1441 gtgaaatgat ggatttatca cgaggtagag ctatcagtcg tgaaaatgaa acgagtcata
     1501 cagcgtcagc gactgtaatg aaatcattgt tgagattgaa tgataccatg gatgattcaa
     1561 caaaaactag atataagcaa atcgttaaaa cttctgttaa ttctgattca agttacaacc
     1621 aaaataatta tttaaattca tattcagaca tagctaaaat gaaaaagtta atgaatgata
     1681 gtactatttc taaaaacgat ttaacacagc aacttaaaat atataatgac atggatcgtg
     1741 tcacctatca caataaagac ctggactttg catttggttt aagtatgaca tcgaaaaaca
     1801 tcgcacgata cgaaaatatc aacggagaga acttaaaagg ttggcacacc ggtgcaggca
     1861 tgtcttattt atataacagc gatgtcaaac actatcgcga taacttctgg gcaacagccg
     1921 atatgacttg tcttccaggc actactactt taaatgatat gccatctact aatactaaga
     1981 atgataaatc ttttgttggc gggacaaaat taaataataa atacgcaagc atcggtatgg
     2041 attttgaaaa tcaggacaaa actttaactg ccaaaaaatc atatttcata ttaaacgata
     2101 aaattgtctt cttaggaact ggcattaaaa gtactgattc atcaaagaat ccagttacaa
     2161 gtgttgaaaa tcgcaaagca aatgggtata aattatttaa agatgatatt gaaattacca
     2221 cttcagatgt taatgctcag gaaacccatt cagtcttttt agagtccaac gatactaaaa
     2281 agaacattgg ttatcatttc ttagacaagc caaaaataac tgtaaaaaaa gaaagtcata
     2341 ctggtaagtg gagtgaaatt aataaaagtc aaaaaaagat gacaaaaaag atgagtatta
     2401 tgaagtaact caaacacata atacatctga cagtaaatat gcatatgttt tgtatcctgg
     2461 tttatcaaaa agtgatttta aatcgaagaa taataatgta agtattgtta aacaagatga
     2521 agattttcat gtgataaaag ataatgatgg cgtatttgct ggggttaatt atagtgataa
     2581 tactaaatct tttgatataa acggaattac tgttgaatta aaagaaaaag gcatgtttgt
     2641 aattaaaaag aaagatgata aagcatataa atgtagcttc tataatcctg aaactacaaa
     2701 taccgcttca aatatagaat
//
LOCUS       DATTQF010000207         2633 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.211, whole genome shotgun sequence.
ACCESSION   DATTQF010000207 DATTQF010000000
VERSION     DATTQF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.211"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(115..414)
                     /locus_tag="VB241_002607"
     CDS             complement(115..414)
                     /locus_tag="VB241_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEP1245822.1"
                     /translation="MSSKDAKKSQEEARLQQLKQEMRNSTDNMVSNAKNNVALKFQTL
                     NEIIETINNSSQSLEGTFEGKASEAAQQSINKLKNNNENLKQKFETLLNTLKING"
     gene            complement(429..2039)
                     /locus_tag="VB241_002608"
     CDS             complement(429..2039)
                     /locus_tag="VB241_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEP1245823.1"
                     /translation="MNLSDALSRSVSDMSYENLDKDQTYDFLGQDYKVIEKRDDTQNG
                     FRAYVFAPVIKGKVDKSQLLIGYAGTDPYSLNDIRTDLQLPIHHNTNNLKINNHYKDL
                     KNSTIHKKYNTIKDKNDLFSFSSNLNNKALFGDLWAAKFAPTQMDESDAFTESVKKKY
                     PDSEIHPLGHSLGAFLAQYNLIKHNLDNGTTFAAPNVYHSFTGDFKKDIDNGVYDSKI
                     RNIGHFDDPINNLNFFNPRIGKNITSMPHYDGFITNLPLIGQHTLKSYNDFDADGNVK
                     EMPELEQFNDDQSLNNRFQLFDPFGIKSRFFDSIKIDSSILNRTSENDFDPFAILNKK
                     DKLFESNDLDIEGFNNKQKEDLSIDLNPFDDELNDEDEPLIPKRGDSKKLKAEKKISV
                     KKTKSSSKNSGKKVIIQPDEVRNIASTIRDRLFLYTQIITALDEYREETMSGANRILD
                     KYQSELLSGSHEFITPNDLEQYMEILSVEGSSGNFKFYNSGLMDDLIQALDQNKKDLL
                     QFAEKLEFAANKFEEKDLEESDVFGLFS"
     gene            2082..2531
                     /locus_tag="VB241_002609"
     CDS             2082..2531
                     /locus_tag="VB241_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1245824.1"
                     /translation="MIRKTKVIILIIILIIAILMMFAYMSHKQKKEHYIAIQEKRIDL
                     FFKHNLNNYKNMKVTDFHKTPMGGYFIVGYINDDKKYKFQASIDSGSNNQYQKNIGYH
                     DDKLGKLFKEKDPKYKLSVDEIIEKEHLDKNEYEADPPLFFFSGPIE"
BASE COUNT          895 a          370 c          370 g          998 t
ORIGIN      
        1 gatactaaaa taagttagat aagaaataaa aattaaaatc ccttcactat ttgtttagcg
       61 aggggatttt agtaatgtat aaaaaagtac agataaatta ataatttctc aatatcatcc
      121 atttatcttc aatgtattta atagggtctc aaatttttgt ttaagatttt cgttattgtt
      181 tttgagttta tttatacttt gttgggctgc ttcgcttgct tttccttcaa atgtaccttc
      241 taaactttgc gaagaattat taattgtttc aatgatttca tttaatgttt gaaattttaa
      301 tgctacatta tttttagcat tgctaaccat attatcagta ctattacgca tttcttgctt
      361 taattgttgt aatctagctt cttcttgact tttctttgca tcttttgagc tcatataaat
      421 taccctcttt aactaaatag tccaaaaaca tcgctttctt ctaaatcttt ttcttcgaat
      481 ttatttgcag caaattctag tttttcagca aattgtagca agtctttttt gttttgatct
      541 aatgcctgaa ttaagtcatc cattaaacca ctattataaa acttgaaatt acctgaactt
      601 ccctcaactg ataaaatctc catatactgt tcaagatcgt taggtgttat aaattcatgt
      661 gaaccgctta gaagctctga ttgatattta tctaaaatgc gattagcccc tgacattgtt
      721 tcttctctat attcatccaa tgcagtaatt atttgtgtat acaaaaatag tctatcacga
      781 attgtgctag cgatattacg cacttcatca ggctgaatta ttactttttt gcctgaattt
      841 ttggaactac ttttggtttt tttaacagag attttctttt ctgcttttaa ttttttacta
      901 tcgcctcttt tgggaattaa tggttcgtcc tcatcattta attcatcatc aaaaggattt
      961 aaatcaatgc ttaaatcttc tttttgttta ttattaaatc cttctatatc aaggtcattt
     1021 gattcgaata atttatcttt tttattgaga atagcaaaag gatcaaaatc attttcactt
     1081 gttctattta atatagaact atcgatttta atagagtcga aaaatctgct ttttatacca
     1141 aatgggtcaa ataattgaaa tctattatta agagattggt catcattaaa ttgttcaagt
     1201 tctggcattt ccttcacatt accatctgca tcgaaatcat tgtaagattt aagcgtgtgt
     1261 tgaccaatca aaggaaggtt agttataaat ccatcataat gtggcattga tgtaatattt
     1321 ttgccaatac gaggattaaa aaaattcaaa ttattaatag gatcatcaaa atggccaata
     1381 ttgcgtattt ttgaatcata gacaccatta tcaatgtctt ttttaaagtc tccagtaaat
     1441 gaatggtata cgttaggtgc tgcaaacgtt gtaccgttat ccagattatg ttttattaaa
     1501 ttatattgag ctaaaaacgc acctaaacta tgtcctaatg ggtgaatttc actatctgga
     1561 tatttctttt taactgattc agtaaaagca tcactttcgt ccatttgagt cggtgcaaac
     1621 ttagctgccc atagatcacc gaaaagtgct ttattattta aattagaaga aaaactaaaa
     1681 aggtcatttt tatcttttat tgtattgtat tttttgtgaa tagttgagtt ttttaaatct
     1741 ttataatgat tattaatttt tagattattt gtgttatgat gaatgggaag ttgtaaatca
     1801 gtacgaatat catttagact atatgggtca gtgccagcgt aacctattaa aagttgactc
     1861 ttatcaactt tacctttgat tactggagcg aaaacatatg ctctaaaccc attctgggta
     1921 tcgtcgcgtt tttcgatgac tttgtaatct tggccaagaa agtcgtatgt ttgatcttta
     1981 tctaaatttt cataagacat atcggaaaca cttcttgaaa gagcgtctga aagattcaaa
     2041 ataaaaactc ctttattaaa aatttaaaag tgaggtaaaa tatgattcgg aaaactaaag
     2101 ttattatttt aattattatt ttaattatag caatattaat gatgtttgca tatatgtcgc
     2161 acaagcaaaa aaaagaacat tatatcgcca ttcaagaaaa aagaattgat ttgtttttta
     2221 aacataactt aaataattat aaaaatatga aagtgacaga tttccataaa actccaatgg
     2281 gtgggtattt cattgtaggt tatataaatg atgataaaaa atataaattc caagcaagta
     2341 tagattcggg aagcaataat caatatcaga aaaatattgg ttaccatgat gataaattag
     2401 gcaaattatt taaagaaaaa gaccctaaat ataaactctc agtcgatgaa ataattgaaa
     2461 aagaacactt ggacaaaaat gaatatgaag ccgatccacc gttatttttc ttttcaggtc
     2521 caattgaata aatgttatgg atagaatgga tgaaaaatat aattattaaa attttaaagt
     2581 aaacctgatc atgatttaag attataagaa agaaaaattg actaatgtta aaa
//
LOCUS       DATTQF010000208         2597 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.212, whole genome shotgun sequence.
ACCESSION   DATTQF010000208 DATTQF010000000
VERSION     DATTQF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.212"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            111..1151
                     /locus_tag="VB241_002610"
     CDS             111..1151
                     /locus_tag="VB241_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEP1245825.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1208..2050)
                     /locus_tag="VB241_002611"
     CDS             complement(1208..2050)
                     /locus_tag="VB241_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP1245826.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSPTYISKSNIKNSNIKVVIGMLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFINGKVGKSDSVLEFYTIDGN
                     IKVE"
     gene            complement(2047..>2597)
                     /locus_tag="VB241_002612"
     CDS             complement(2047..>2597)
                     /locus_tag="VB241_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP1245827.1"
                     /translation="ITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEEEIL
                     KKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSIVGL
                     FVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKILEY
                     IYRLILKYLLWYIKTVKGSVRK"
BASE COUNT          919 a          449 c          350 g          879 t
ORIGIN      
        1 ttttatattt catttagtaa aatataactt tatttaacat gacattcaat atacatacga
       61 ttactattta tttacaatat attacaacta aaaagaaagg ggccgatgat atgcaacaag
      121 tgacatcaga tattatgact tttagaggtt cacatttcga gttaggtgtt aaaactggta
      181 aatggcttca acaaacacct cttctaaaaa atagagaaaa agaatggaaa aaacgtgtcc
      241 cacgtttcga tatagatgta aatgaaactt atcaaatatt ccaaacttac gcacctcaaa
      301 tatgggaaga attaatggga ctacaaagta tactcaaaat gccaacacgt caaattattt
      361 taaactttgg acattatcgt tttaccgatt taaaagaaag tggttgcact gtctttcaag
      421 gcaaagactt catggttaga aattacgatt accaccctgc aacttatgat ggacgctact
      481 tactatatca acctacagat agcggtttag ctcaaattgg tcctgtatca agggtaactg
      541 gaagaatgga tggtatgaat gagtcaggat taactatggg ctataacttt atgcaccgga
      601 agaaaccagc aaatggtttt gtatgttata tgattggtcg tctaattctt gaaaattgcc
      661 gaaatgtaac agaagcaatc caattattga aagaaatacc acacagaagc tcatttagct
      721 acatactgat ggacaaatca ttgaaccatg caattgttga agtgacgccg agatcaatag
      781 atgttcgtta tgataatata tgtacaaatc attttgaaat attaacacat gaaaatcgca
      841 attatacaaa agaatctaaa gaaagactag cgcgtaccat ttcacaaact aatgataatt
      901 tagatatgac tacagcattt aagttattca acaatcctca gtatgaaatt tacagtaaac
      961 tattcaaaag ctggtctggc accatccata catcaatgta tcaccctgaa acattaactg
     1021 catattttac attgggagaa aatgcgccac cagaaataat agatttcaaa tcatggttag
     1081 atggtcaaga ccttaatatc actcacttta ctggcaatat agataccgac ttaacttttg
     1141 ccaacaaata aataacaccg atattagaga aattatgctt cctaatatcg gtgcttacat
     1201 cctttattta ttcaactttg atattaccat caatcgtata aaattctaaa acactatcgc
     1261 ttttcccaac tttaccatta ataaacgttt tatttttaac tacgctatca cccgttcccg
     1321 gatttagctt cagtatagta tcttcaggtt tactatcata cttaaaacga atatctcctt
     1381 gtttagtaga agcttttgca tctacttttg atggcatgtt tttaaattca atgtcaccat
     1441 gatcgtttaa aaatatggat tgtttaattt gactcttgtc gatttgtagc ataccaataa
     1501 caactttaat attactattt ttaatgttac ttttactaat ataagtagga ctattatttg
     1561 tctttgaatc tagtgcgtca agatttgagt ttttaataac tacttcaccg ttaatgcttt
     1621 gaatacttgt gttctctaaa tcaacatcac tgattctaac acttcctgct ccagacgata
     1681 tatttaaacg tttaatcatt ttatcaggca tttcaatcgt taacgttttc ttattctcat
     1741 gaaaaggatt catatcaatt gcatatcctc ttgtcttaga ccttttatca ctaatcttca
     1801 acgtcttatc tactatatta attaatatat cattgtcacc atcatattta actctaaatt
     1861 gattcccccg ctttatacga agttctgtac tatccaaatt tatagataca ttgtcaaaag
     1921 catcgtcttt gaatgtttta tcatattgtt tagtaccata tttgttttta tcatagctga
     1981 accaaataat ggttgctgct gtaaaaaaga caacaaacac taaaagccca ataaaaaaga
     2041 atttcttcat tttctaacgc ttcctttgac agttttaata taccaaagta aatattttaa
     2101 gattaaacga taaatgtatt ctaaaatctt aaatatgaca atgataaata ctaatcctaa
     2161 tcctgaatat gatatggcaa aaaatacatt actaattgag tctacaattc ctctggatat
     2221 tgctgatgct aataaaataa ttggacaaaa cagcagcaaa agtgatatta aaaataatgc
     2281 taatacaaat aatccaacta tagatactgg tataaggatg acaaaaatag atagaatgcc
     2341 taaacttaat gaagcagcaa cagctcttga aatattttca aaagttggtt tagattcagc
     2401 ataatcaata acacttctag cttttgtttc agctgcaaca tcttgtgggt cttttaactt
     2461 ctttaaaatt tcctcttcat caacgccatt aagttcttgc tcataaaaat aacgttcata
     2521 ttcatacatt ttttgatctc ttaaatgcct aggtagcgca tcaagtgctg tttctaattc
     2581 atttaaataa gtaatct
//
LOCUS       DATTQF010000209         2593 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.213, whole genome shotgun sequence.
ACCESSION   DATTQF010000209 DATTQF010000000
VERSION     DATTQF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.213"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            117..887
                     /locus_tag="VB241_002613"
     CDS             117..887
                     /locus_tag="VB241_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245828.1"
                     /translation="MGNIKSFALYISILLLIVVVAGCGKSDKTKEDSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKFSDRKNYKVEMKNNKIILLDKVNDPNLKERIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            919..1719
                     /locus_tag="VB241_002614"
     CDS             919..1719
                     /locus_tag="VB241_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245829.1"
                     /translation="MRYLNRVVLYIIVMVLSVFIIGCDKSSDTSEKPKEDSKEAQIKK
                     SFEKTLDMYPIKNLEDLYDKEGSRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVL
                     YLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRIENFK
                     FFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVL
                     KMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVNEDDE"
     gene            1738..2532
                     /locus_tag="VB241_002615"
     CDS             1738..2532
                     /locus_tag="VB241_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245830.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKITGDSKEEQIKKSFAK
                     TLEMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVIRSEMKIQLKGENLESRGVVLEINR
                     NTRTAKGNYIVREVVEDSDGMTHNHTKRYPVKMENNKIIPLKQIVDEKVKKEIEEFKF
                     FVQYGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFVENKGENIYFTDSVYFNPSEDKYNY"
BASE COUNT         1109 a          253 c          501 g          730 t
ORIGIN      
        1 attaaattga ttgtatatgt atattttggt aacgtaaaag agaaatatac aaaataatta
       61 attatttata tgaaaagaga atataaatga agtataaaac agagagacgt gaaatgatgg
      121 gaaatataaa aagttttgca ttgtacataa gcatcttgct tttaatagtt gttgtagcag
      181 gttgtggcaa aagtgataaa acaaaagaag attccaaaga agaacaaatt aaaaagagct
      241 ttgcgaaaac attagatatg tatccaatta agaatctcga ggacctatat gataaagaag
      301 gatatcgaga tggcgaattt gaaaaaggtg acaaagggat gtggacgata tatacagatt
      361 ttgctaaaag caataaatca gacgaattgg atgatgaagg tatggtttta aatctggata
      421 gaaatactcg aacggctaag ggatattatt ttgttaagaa attttatgaa aaggataaat
      481 tttcagatag aaaaaattat aaagttgaaa tgaaaaacaa taaaattatt ttattagaca
      541 aggtaaacga tccaaacctt aaagaaagaa tagaaaattt taagtttttt ggacaatatg
      601 caaattttaa ggatttggaa aattacaaca atggcgatgt gtcaataaat tggaatgttc
      661 caagttatga cgtggaatat aaaatgagca ataaagatga aaatgttaag caattaagaa
      721 gtcgttataa cattcctact gataaagctc caatgttaaa aatgcatatt gacggggact
      781 taaaaggaag ttctgttgga tataaaaggt tagaaataga cttttcaaaa gaagataggg
      841 atatttcagt cattgattat ttaagttata agccagcgaa aaaatagtgt tgataattaa
      901 atattagggt gtgaaatgat gagatattta aatagagttg tactgtacat aattgttatg
      961 gttttgagtg tttttataat aggttgtgat aaatcaagcg atacttcaga aaagccaaaa
     1021 gaagattcaa aagaagcaca aattaaaaag agttttgaga aaacattaga tatgtatcca
     1081 attaagaatc tcgaggattt atacgacaaa gagggatctc gtgatggtga gtttaaaaaa
     1141 ggcgataaag ggatgtggac gatatataca gatttcgcca aaagtaataa acaaggtgga
     1201 ttgagtaatg aaggtatggt cttatactta gatagaaata cacggacagc aaagggacat
     1261 tattttgtta agacatttta tgaaaaggat aaattcccag atagaaaaaa atataaagtt
     1321 gaaatgaaaa acaataaaat tatcttatta gacaaggtag aagatccaaa actaaaaaag
     1381 agaatagaaa actttaaatt tttcggacaa tatgcaaacc ttaaagaatt gaaaaattac
     1441 aacaatggtg atgtctcaat taatgagaat gttccaagtt atgacgcaaa atttaaaatg
     1501 agcaataaag atgaaaatgt taagcaatta agaagtcgtt ataatattcc tactgataaa
     1561 gcaccggtat taaaaatgca tattgatggt aatttgaaag gaagttctgt gggttataaa
     1621 aagttggaaa ttgacttttc aaaaggtgaa aaaagcgatt tgtcagtaat agattctttg
     1681 aatttccagc cggcgaaggt gaatgaagat gatgaatgat gaggatggtg tgtaacaatg
     1741 aagtctataa aaaggattgg attgtgcatt agtttgttga ttttaatcat ctttgttaca
     1801 tcttgtgatg gtgataataa gattactgga gactcaaaag aagaacaaat caaaaagagc
     1861 tttgcgaaaa cattagaaat gtatccaatc aaaaacctcg aagatttata cgacaaagaa
     1921 ggatatcgag acggagaatt taaaaaaggt gataaaggga cttgggttat tagatctgaa
     1981 atgaaaatcc aattaaaagg agaaaatctg gaatctaggg gagtagtttt agaaattaac
     2041 agaaatacta gaacggctaa agggaattat attgttagag aagttgttga agatagcgat
     2101 ggaatgacac acaatcatac aaaaaggtat cctgtaaaaa tggaaaataa taaaatcatt
     2161 ccattaaaac aaatcgttga tgaaaaagta aaaaaagaaa ttgaagaatt taaattcttt
     2221 gtacaatacg ggaatttcaa agaattggaa aactataaag acggagaagt gacatataac
     2281 ccagaagcac caatatactc tgcacaatat caattgaaaa acagtgatta caatgtagaa
     2341 caattacgta agcgatataa tatcccgacg caaaaagcgc ctaaattatt attgaaaggg
     2401 tcaggtaatc taaaaggctc atcagttgga tataaaaata ttgaatttac ctttgttgaa
     2461 aataagggag aaaatattta cttcacagat agtgtctact ttaatccaag cgaggataaa
     2521 tataattact aatataaaaa ataacgttag aacatttaat aataaagtta aatgaattac
     2581 atgtattaaa ttg
//
LOCUS       DATTQF010000210         2592 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.214, whole genome shotgun sequence.
ACCESSION   DATTQF010000210 DATTQF010000000
VERSION     DATTQF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.214"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..226
                     /locus_tag="VB241_002616"
     CDS             <1..226
                     /locus_tag="VB241_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245831.1"
                     /translation="ILRFHGVSKRKICKMYNFAYFRILEVCEMAKKNDYRFTYKDYKF
                     LKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            377..697
                     /locus_tag="VB241_002617"
     CDS             377..697
                     /locus_tag="VB241_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HEP1245832.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            711..1013
                     /locus_tag="VB241_002618"
     CDS             711..1013
                     /locus_tag="VB241_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245833.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1255..2280
                     /locus_tag="VB241_002619"
     CDS             1255..2280
                     /locus_tag="VB241_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEP1245834.1"
                     /translation="MEAVVDWVQVTFHITPISAVIEDVIGLPITLFKKRNSGIYFYNR
                     GYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVNANFT
                     RIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRSGSAINGFNLSDGRSKGATFYIGSKQ
                     SNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKVLSRTDNISEIVK
                     SILHNNLRFISPPKDGNDKNRKRWPLYRPWALFIKDTEKLNLTTRPTLKSIEDNLDWL
                     CKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQFNDEHTNRINHYLEALKQKK
                     HLSKDKC"
     gene            2341..>2592
                     /locus_tag="VB241_002620"
     CDS             2341..>2592
                     /locus_tag="VB241_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP1245835.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYED"
BASE COUNT          942 a          311 c          497 g          842 t
ORIGIN      
        1 aattttaaga tttcatggtg tttctaaaag aaaaatttgt aaaatgtata attttgcata
       61 ttttcgtata ttagaagttt gtgaaatggc aaaaaagaat gattatcgat ttacttataa
      121 ggattataaa tttttaaaac aatatggtgt gtctaataca tttatttgta agatgtatca
      181 tatttcaatt gaagacttag aattttttga ggtgatgaac cgataggttt gattaaaaat
      241 atgataggtc aggagaagca tagacactcc attatcaaaa atgcagtggt acggatttgc
      301 gggagttatt cctattcgtg cgacccttta gaagtcgttt aattttatta aaaattttgt
      361 aaaggatgga tatattatga aaccagtatt ggatattaaa aaaactttag gacaattaaa
      421 ctttttaggt gtagatgaaa aatataaata tgaaaatgga gagcgtactg ataaaactgt
      481 ttatgcatat aaattagcga gccaagagca aggcgaacaa atcactgtta aaacgcctaa
      541 taaagtagaa ttagactatt tacaagaatg cgaattagtt gaaccagacg ttaaaatgta
      601 tgttcaaatg tcaggcgatt ttggaactat cgcatatagc tggaatgcag aagatattaa
      661 agtagttggt tctaatagtg cacttaaaca aaaataatta ggtgatgtaa atggattatt
      721 atacagcaga tagattatat cgctacacaa atagttcaaa tttgagtgaa cctattttaa
      781 actatgttgc tagtcgtata aattggggcg ataaagtttc attaatgact ttagctaaag
      841 aaatacagtc taaatttaat gatagttacg ttaaagaaaa tacggtaaaa ggtcggccta
      901 aaatttacgc tgacctttgt ctattgtgta tgagcttgag tgaagcagga catggcagaa
      961 tgctacaagt taatttggaa gattgtattt atattggcga tattgatgtt taacatatag
     1021 tattagtgat ttaacatgac cgtgtagctt ttagcgtatg caggcgcaga aatgcaagca
     1081 cggagtgcgt taagcatttg ggcggagcct gcgacaaata cgcgttaaaa aagcgagaac
     1141 ggtcatgtat caaatcgctt gcgatttgat aggaggcata aataaatatg actttaaaaa
     1201 accagtgtac cccccttact aatagggggg gtagagcgta caaataaaag tgccgtggag
     1261 gcagttgttg actgggttca ggtcactttc catataaccc ctatttcagc agtaattgag
     1321 gacgttatcg gtttgcctat aacgttgttc aaaaaacgaa atagtggcat ttatttttat
     1381 aatcgcggtt atgaattttc aaacattaaa ttgtattatt caagtgatga tgaatcaatg
     1441 ggcattcact tacagttaac tggtacaggt tgtagagagt ttgaacatca tttacaacag
     1501 ttgaataaaa cttggcaaga tttttttgat aaatgtttat cagttaatgc aaattttaca
     1561 agaattgata ttgcaattga tgattataaa acatatttaa aagtgccttt attaattaaa
     1621 aaggctgaaa aagctgagtg tgtttcacgt tttagatctg gaagcgcaat aaacggtttt
     1681 aatttgtcag acggaagaag taaaggcgct actttttata ttggttctaa acaaagcaat
     1741 ttatattgtc gtttttatga aaagaattat gaacaagcgt ttaaacgtca ttgtgatgtc
     1801 gaagacattg ggttatggaa tcgttatgaa atacaaatgc gaaaggcata tgcagttaat
     1861 tgtgcgaaag ttttgagtag aacagataat ataagcgaaa ttgtaaagtc tatattgcat
     1921 aataatttgc gttttatctc ccctcctaaa gatggaaatg ataaaaatcg taagcggtgg
     1981 ccattatata gaccttgggc gttatttatt aaagatactg aaaaattgaa tttaactact
     2041 agaccaacat taaagtccat tgaggataat cttgattggt tatgtaaaca agttgctact
     2101 actttagata cagtgttaac tgccgaaagt atggcgcaat ctgaggggtt gcttacagat
     2161 actgattttt tagataaaat tttagcgcat tcacaattta atgatgagca tacgaataga
     2221 attaatcatt atttggaggc tttaaaacaa aaaaagcact tatcaaaaga taagtgttaa
     2281 aaaaataact gggatgtgat ttttattaaa aatttcgtaa aggattttag aaaatcgttt
     2341 atgaaaataa taagcaagta cattttaaca cgattttcta aaaacggaaa gcattttgat
     2401 attcctaaag aacaacgccg agtaattcca aaatttaaaa atcgtcgtaa gatgattgta
     2461 atttgttttt actcattatt agctattttg ttaattcttt tattagcttc ttttattaaa
     2521 gctacaagag caaataatga tagtaaagaa gcagttaata aaacgaatat gatccaaaaa
     2581 aagtatgaag at
//
LOCUS       DATTQF010000211         2565 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.215, whole genome shotgun sequence.
ACCESSION   DATTQF010000211 DATTQF010000000
VERSION     DATTQF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.215"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            42..434
                     /locus_tag="VB241_002621"
     CDS             42..434
                     /locus_tag="VB241_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEP1245836.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            436..>2565
                     /locus_tag="VB241_002622"
     CDS             436..>2565
                     /locus_tag="VB241_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEP1245837.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFDLLWQNSTQLTQQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPTKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEK
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFEMFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLREGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDALLTENESKN
                     LVFFSRQIAPGLKRIDSIVLEELLKNEL"
BASE COUNT          998 a          281 c          467 g          819 t
ORIGIN      
        1 gaataacaat taagatgatt ttaaattgaa gggtgagtct tatgaaaaaa gtaatcaatg
       61 tagtaggagc tattattttt tctgataaca aaattctttg tgcacagaga agtgaaaaaa
      121 tgagtctgcc tttaatgtgg gaatttcctg gcggtaaggt tgaaaagaat gaaactgaaa
      181 aagacgcttt gattagagaa attagagaag aaatgaaatg tgatctaatt gttggagaca
      241 aagttataac tacagaacat gaatatgatt ttggaattgt taggttaaca acatacaaat
      301 gtactttaaa caaagagtta ccaactctaa ctgaacataa gagtattgaa tggttgtcaa
      361 taaacgaact tgataaatta aattgggccc cagcggatat accagccgtt aataaaatta
      421 tgactgaggg ataatatgag tagattacta aatgatttca atcaatcttt acataaaggg
      481 tttatagaca aacatatttc tcataaaggg aattatacac cgaagctttt agtaaataat
      541 aaaaatgaaa aagttctttc aacaattata gatgaacttc aaaaatgtga aacgttctat
      601 ttttctgtgg cttttataac agaaagcggt ttagccagct taaaggctca actattagat
      661 ttaagcaata agggtgttaa agggaaaata ttaacatcta attacttagg gtttaatagt
      721 cctaaaatgt atggagaatt acttaaatta aaaaatgtag aggtaagatt aaccgatatt
      781 gctggattcc atgccaaagg atatattttt gaacataagg attatagttc tatggtaata
      841 ggaagctcta atttaacatc gaatgcgtta aaggttaatt atgagcataa tgttttactg
      901 tctactatga aaaatgggga tttggttgat agtgtaaaaa atgaatttga cttgttatgg
      961 caaaacagta cccaactgac tcagcaatgg attaattcat ataaagaatc atttgagtac
     1021 cgctccttag aaaaattggc ggaggttgaa caaactcaaa tgctgctagc tgataaggtg
     1081 aaaaaatcag tggaaattgt accgaattta atgcaagcag aagcattaag gtcattaaaa
     1141 gctattaggg ataaagccaa agataaggcg ttaataatat ctgcaactgg tacaggtaaa
     1201 acgattttat gtgcattaga tgttagagaa gtaaacccta ataaattttt atttattgtt
     1261 cataatgagg gaattttaaa tagagctaag gaagaattta aaaaagtatt acctacaaaa
     1321 aatgatagtg attttggatt gttgacaggc aaacatagag atgtcgatgc caaatattta
     1381 tttgcaacaa ttcaaacatt atctagagat gataatttta agcagtttga tgaaaaagaa
     1441 tttgattata ttgtttttga tgaagcacat cgttctgctg catctactta tcaacgagta
     1501 tttaattact tcaaacctaa gtttatgctg ggtatgactg caacaccaga aagatcagat
     1561 gaattaagta tatttgaaat gtttgattat aatattgctt atgaaataag gttacaagca
     1621 gcattagaaa gtgatatttt atgtccattt cattattttg gtgtgactga ttatgtacat
     1681 caaggtatta aagaagatga tgtaactaaa ctgagatatt taacttctga tgaaagagtt
     1741 aattatatta ttcaaaagac agattactat ggatattcag gtgaaatttt acaaggatta
     1801 atttttgtaa gtagcaaaaa agaagcttat gatttagcgg ataaactaag ttcaaaaggg
     1861 attaaaagtg ttgctttaac aggagacgat agtgttaatt atcgccaaat tgtaatagaa
     1921 aaattaagag aaggtaagat taactatatt ataactgtag atctatttaa cgaaggtatt
     1981 gatattccag aagtcaatca agttgtaatg ctgagaccaa ctgagtctag tattatattc
     2041 attcagcaac ttggtagagg gttaagaaaa agttcaaata aagagtatgt tacagtaatt
     2101 gattttattg gtaattataa gacaaattat ttaattccga ttgcgctttc tggggatcaa
     2161 tcgcaaaata aagataatta taagaagttt ttaacgaata acgattcgat taatggagta
     2221 tctacaatta attttgaaga agttgctaaa aaacagattt acaattcatt ggatgcagta
     2281 tcattgaatc aaaataaatt aatattaaaa gcttatgaag aagttgaaaa tagattggga
     2341 cacatgccgt tactaatgga ttttatacaa caacattcaa tagatccaag cgttatattt
     2401 tctaaattta gtaattatta cgaattttta gtaaggtata aaaaaataga tgctttattg
     2461 acggaaaatg aatctaaaaa tctggttttc ttttcaagac aaatagcacc tggattgaaa
     2521 agaatcgata gtatagtttt agaagaactt ttaaaaaatg aatta
//
LOCUS       DATTQF010000212         2533 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.216, whole genome shotgun sequence.
ACCESSION   DATTQF010000212 DATTQF010000000
VERSION     DATTQF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.216"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(121..1275)
                     /locus_tag="VB241_002623"
     CDS             complement(121..1275)
                     /locus_tag="VB241_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1245838.1"
                     /translation="MKLSLNSNSKFLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPNIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(1470..2480)
                     /locus_tag="VB241_002624"
     CDS             complement(1470..2480)
                     /locus_tag="VB241_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEP1245839.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
BASE COUNT          909 a          365 c          374 g          885 t
ORIGIN      
        1 agaggaatat aactcggtag tatttacagt ctctttagag tattatttaa aacatataga
       61 atataaaaaa ctgaacgtac tatgaatttc gttcacgacg tccagttcaa catatcattt
      121 ttatttagta tttaaatatt tagcaagtct cttcatacct tcttttaaaa catccatttc
      181 ataagcatag gaaatcctca caaatccttt accgaattct gtaaaggatg agcctggaac
      241 gattgctaaa tgtgttgatt caagtaaatc gacacaaaat tcgaaatcgt catcggtgat
      301 atgcttaata tttgggaaaa tataaaacgc gccttcaggt tgagcggtaa tctcaaaacc
      361 taatttagtt aattcagata ctaaataatt tcttcgttct acataagctt cattcatata
      421 tttaggagct tctaatcctt cgttaagtgc tgttatacat gctatttgag ctggaacatt
      481 ggcacaaata caattatagg cgtgcataaa tgttaattta tcaatcaaat attgaggtcc
      541 taatagaaaa ccaattctta ttcctgttgc tgagtgtgat ttacttaaac caccaattaa
      601 tattaattga tcacgaatgt cttcaaattc agcgaaggat acatgtttac cactaaatgt
      661 attttcagca taaatctcat cgctaataat aaatatcgga tattttttta atacatttac
      721 gatatttaaa acttcatttc tttttaaaac tacgccagtt ggattagtcg gataatttaa
      781 caagacagct tttgtctttg gagaaatatg actttctaac gcatcaggtg taattttgaa
      841 ttgtgttgct gttgtatcaa tataaattgg tttaccacct agtacttcga tgagtggtat
      901 gtagcctgca taaattggtc ccggtattat aatttcatct ccaggctcta tgatagaacg
      961 taacgttgtg tctattgctt cacttgctcc atttgtcaca ataatttctt caggatcata
     1021 ggaaaaatga taacgatttt tgaagtattg actaatagct tcgcgagttt ctaataagcc
     1081 tttattgtga gagtaagatg tcttgtcatt gttaatagca tctatatatg cctttttcac
     1141 aacatcaggc atagggaagt ctggttggcc aatagttaaa ttaacacaat catccaaatt
     1201 attcatacga tttgaaaatt ggcgaatact tggtgctctt aaaaatttag aattagagtt
     1261 taaagaaagt ttcatcgtga cacctcaaat ataaatcaaa tattgtctaa aaatttagaa
     1321 attaataata tcataacata cttttaaagc aaaaaagggt aaattactta agctttattt
     1381 gaaaaatgaa aaatgtctaa atacactgta gtaactactt ttcaaaatga atgaagtggt
     1441 tacagttaat gtacttagac attatataat tatgagtctt taaactgtga tggtctttgt
     1501 aaattaatta ttggatttgt ccatttacaa acaaagtttg tagataatac atatacgatg
     1561 attacagata tacttattaa ataaagatac gtcattaatg atataggatt atcgaatggg
     1621 taccattcaa atcctctaac aatgccaata attaaaccat gtaataaata tacgtatagc
     1681 gtacgactgc caatataagt atataatttt ttctttgttg acattaaatt tagaaacgca
     1741 gtcattgcga ttaatataat tccatataat ataagtcgtt taaaaggact gaatacactc
     1801 tgtccttcat tttcaagtga agtatatggt gaacttccta ataaccaatc tgcattgata
     1861 ggatgaatca cgtaaatgat aaaaaataaa ataaacgtaa tgatagatac tggtattagt
     1921 tttttatttt taaaaatagc cgtatgtttt ttggtgaaaa tgtagcctag gtaaaatatt
     1981 gggaaaaata cgattgtcct tgaaatgctt aagtagctat cgatgttatc tgaaaaacct
     2041 gctccaatag atataataat tgaaactgat agcactttat atggattaaa tcttctaact
     2101 attactaaaa tgacatgaaa gaaaaatagc gtgatcaaaa accataacgc aaatactggg
     2161 ttaaaaggat caagttgtaa ttcgtcactt ttacctgtta agaaataata tattgaaaag
     2221 aatgcaaaaa atatcatata aggtactatc aagcgttttg aaattttttc taaatagtat
     2281 ggtttatcaa tatttttcgc gaaataacca gatataaaca aaaatgttgg catatggaaa
     2341 ctataaataa ctaggtataa tgctgataaa tatttatcgc cactagtgta gggttgtaac
     2401 atatgtccaa atacgactaa taatattaaa attgctcttg cgttgtcaaa aaaataatct
     2461 ctctctttta atgaagtcat attttatctc ctttatgtcc ataagactct tactaattta
     2521 ttatattttg tat
//
LOCUS       DATTQF010000213         2429 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.217, whole genome shotgun sequence.
ACCESSION   DATTQF010000213 DATTQF010000000
VERSION     DATTQF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.217"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..318
                     /locus_tag="VB241_002625"
     CDS             <1..318
                     /locus_tag="VB241_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1245840.1"
                     /translation="FFKYNLNHYKSLKVTRFKKSPMDSYFIGGYINNNKDYEFQAFIN
                     VGGRKQFQRDIGYDEEKLGKLLKEQEVKTWKTVDQIIKNEHLDKSEYEAEPPLFFFSG
                     SLD"
     gene            complement(493..594)
                     /locus_tag="VB241_002626"
     CDS             complement(493..594)
                     /locus_tag="VB241_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245841.1"
                     /translation="MQGFKEKHQELKSALCQIGLMRSISEVRQLNIA"
     gene            complement(717..812)
                     /gene="pepA1"
                     /locus_tag="VB241_002627"
     CDS             complement(717..812)
                     /gene="pepA1"
                     /locus_tag="VB241_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HEP1245842.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1263..1709
                     /locus_tag="VB241_002628"
     CDS             1263..1709
                     /locus_tag="VB241_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HEP1245843.1"
                     /translation="MKKRYVFVVVVALLIVLIVIAYAFHKSKKEHYIETQEKRIDLYF
                     KHNLNHYKSMKITKFQKSPVNAYFIKGYINNDKQYKFQAYINTGDEGNQFNSSIGYKE
                     EKIGRLLKEKDAKDRLTVDEIIEKEHLDKNEYEAEPPLFFFSGSLD"
     gene            1902..>2429
                     /locus_tag="VB241_002629"
     CDS             1902..>2429
                     /locus_tag="VB241_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP1245844.1"
                     /translation="MTAIDKVYYEDVLEDDILNLLKPYIQGKEKELYITSDNFAIDLN
                     KVTALLDLKIQYEDLDDDVSGYIDERTIYVNKNHSSNRKRFTIAHEIGHFLHNHKNKI
                     KYRNNSYAYDTEEMIEERVANDFSANLLMPKKQMEALVYKFYKENNINLTSGLSISER
                     EELINYIAIKLEVSKI"
BASE COUNT          960 a          288 c          409 g          772 t
ORIGIN      
        1 ttttttaagt ataacctgaa tcattataag tcacttaaag taacgagatt taaaaagtcg
       61 ccaatggaca gttattttat aggtggttat ataaataaca ataaagacta tgaattccaa
      121 gcttttatta atgtaggtgg tagaaagcaa tttcaaagag atataggata tgacgaagaa
      181 aaacttggaa aactattaaa agagcaagaa gtaaaaactt ggaaaaccgt cgatcaaata
      241 attaaaaatg aacacttaga taaaagtgaa tatgaagcgg aaccaccgtt gtttttcttt
      301 tcaggatcac tggattagat tcggttgtat ggtgtaataa aaattattat tatcctcaca
      361 atcattaaag gacaagagta ttttaaaaaa tcattacata acatacaaat tttggttatg
      421 gaagttacaa tcatttgcgc aatcgaatat tattatgttt aaaactttac gctccaaaaa
      481 gtaaaaagga agttaagcaa tgtttagttg cctaacttcc gatattgaac gcatcagtcc
      541 aatttggcat agagcactct ttagttcttg atgtttctct ttaaaacctt gcatatttta
      601 caaaaagaaa aattagcagt ataattgaga taacgaaaat aagtatttac ttatacacca
      661 atcccctcac tatttgcggt agtgagggga tttttattgg tgcggctata tgtcacctat
      721 tttgtattgc gtctacttag ccaataagaa aaaaacgcaa tggcacagcc actgatgact
      781 ggtgctatga tgtgaacgaa aataagcatc accttataca cctcctctct gcgtctaaat
      841 tgacgcctga gagataggcg actctactat tatatcatct aattggacaa attctatgat
      901 agtagatttt gttaatttaa gaaagaagaa catttagttc agagtttaag ttataaatgg
      961 ttagattata tgaaaatggt aagccaagga atgaaattat acgcgagtat gatttaacat
     1021 ctttgatgtt aggaaaaaca gcattttcat ttcgccattt tcctacattt tataaaccca
     1081 tatataacag cgttatttta tgtatttata attatgtcaa gcaaataatt tttaaaagta
     1141 ttttgaaaca acgtttcatg aggggcgtaa acggaacata gaaaaaagat agcatgtcaa
     1201 agtgaattgt tttttgaaat ctaaaatcta taagaaataa atactaaact atgggggtta
     1261 aaatgaaaaa gagatatgta tttgtggtag ttgttgcatt attaatagta ctaattgtta
     1321 ttgcatacgc gtttcacaaa agtaaaaaag aacattatat tgagacacaa gaaaagcgaa
     1381 tcgatttata ttttaagcat aacctgaatc actataagtc tatgaaaata acaaagtttc
     1441 aaaaaagccc tgtgaatgct tactttatta aaggatatat aaataatgat aaacaatata
     1501 aatttcaggc atatataaat acaggagatg aaggaaatca gtttaatagt agcataggat
     1561 ataaggaaga aaaaataggg cgattattga aagagaaaga tgctaaagac cgattaacag
     1621 tggatgaaat aattgaaaaa gaacacttag acaaaaatga atatgaagcc gagccaccgt
     1681 tgttcttctt ttcgggatcg ttggattaga ttcggttgta cagtgaaaaa atattttatt
     1741 attaatttta gtgattaaaa caataaatgt aagtaaagat atgcgcatat ttaattagtg
     1801 actaaaataa tgaaagttag actggttatt ttaagcttta gaatataaaa tataattaat
     1861 agatagagaa gattaacaat atcttttagg aggcgatttt tatgactgcc attgacaaag
     1921 tttattatga agatgtatta gaagatgaca tacttaatct gcttaaacca tatatacagg
     1981 ggaaagaaaa ggaactttat atcacttctg ataattttgc aattgattta aataaagtga
     2041 ccgcattact ggatttaaaa attcagtatg aagatttgga tgatgatgtt tcgggttata
     2101 ttgatgaaag aactatttat gtaaataaaa atcattctag taatagaaaa agatttacaa
     2161 ttgcccatga aataggacat tttttacaca atcataaaaa taaaatcaaa tatagaaata
     2221 atagttatgc atatgacact gaagaaatga ttgaagaaag ggtggctaat gacttttcag
     2281 ctaatttact aatgcccaaa aagcaaatgg aagctttagt atataagttt tacaaggaaa
     2341 ataatattaa tttaacttct ggtttatcaa tttcagaaag agaagagtta attaactata
     2401 ttgcaattaa gttggaagtt tctaaaata
//
LOCUS       DATTQF010000214         2341 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.218, whole genome shotgun sequence.
ACCESSION   DATTQF010000214 DATTQF010000000
VERSION     DATTQF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.218"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(307..1314)
                     /locus_tag="VB241_002630"
     CDS             complement(307..1314)
                     /locus_tag="VB241_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEP1245845.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1330..1947)
                     /locus_tag="VB241_002631"
     CDS             complement(1330..1947)
                     /locus_tag="VB241_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEP1245846.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          819 a          405 c          318 g          799 t
ORIGIN      
        1 atacaaattt acattaaata aagtccaata caatttaggt taattaaaca agttgatagc
       61 tatttaatta ttcattcatt gaagaatata aactgttaaa tcattatttt acgcttactt
      121 atattttaat gacctaactt attatgttcc atggaataca tttataactt agcccctaat
      181 taaaatgctt tgtcttggtc attctatgta aactctataa aataggttgt ctatttacat
      241 aaaaaaatga acttcaatac caccatatat ttgtgatact gaagttcagt atagcttaat
      301 tttcaattag aaaaataaat taagtatata gaataataaa cctgctaaca atgctgaaat
      361 aggtaatgta atcacccatg taatgatcat tcgttgcgca gtactccatt ttacaccttt
      421 agctcggtta gaagcaccaa cacctaagat tgatgatgac acaacgtgag ttgttgataa
      481 tgggaaatgt agcgatgatg caacaaaaat tgttaatgca gatgataaat cggccgcagc
      541 accgtttgct gggcgtattt tcataatatt accacctaca gttttgataa ttttccagcc
      601 accaattgca gtaccaagcc ccattgctgt cgcacaggca aattttaccc ataactgtgg
      661 ttcaacgctg ccatcattct gtacattagc aacaatcaat gccaacgtaa taatacccat
      721 tgatttttgc gcatcattcg taccgtgaga gaatgattgt aacgctgctg tgaaaatttg
      781 gaaaaatcta aagttacgat tcgctcttgt taaatttgca tttttaaaga taactttaaa
      841 aattgaatac atcaagaaac caacacaaaa tgcgataatc ggtgaaacga ttaatacaat
      901 aataattttt gtgaaacctt ggtaatgtaa tacgccaaat gagccttcag atgcgattgc
      961 tgcacccgca attgaaccta taagtgcatg tgaagacgaa cttggaattc cgtaaaacca
     1021 agtagctaaa ttccaaataa tagccgcaag tattgcagct aacacaacaa ctaatccatt
     1081 ttccaattta aatggatcga caatgtcttt agtaatggtg cctgcaacgc ccgtaaatgt
     1141 taaagcacct ataaagttca tcactgctgc cattaaaatt gccgttttag gcgttaacgc
     1201 tctagtagat acagcagtag ctactgcatt ggctgtatca tggaatccat tgataaagtc
     1261 aaatatcagc gagaaaataa ctacagctat agtgacgatg attatatatg acataaatat
     1321 atactcccct tagctatttt tcataataat agtttcaaaa ttatttgcta cgatttgaca
     1381 tttatcagca atttcttcca tgctttcata aatatctttt attttaatta atgtgattgg
     1441 atctgtttca ctattgaaaa tatgtttaat tgactgtctt aaaataccat cacagtttgt
     1501 ttcaaattct ttaatattaa ttgaatgaat acgcatatgt gataattttt tatcgactaa
     1561 taagccgaca gcaagtttca tttctgcaac tgctttttga atgttatcaa caaactcagc
     1621 catatattca tctgtgtatt cgattgaata catttcaaac atagctgccg tttcttcaat
     1681 tgcatctaaa acatcatcaa ttgcatcaca taatgataaa atatcttcac gttcaattgg
     1741 tgtgataaat gtttgattta aatcagtaat tacttgatgt actaattcgt caccatgtga
     1801 ctcataagtt ttaatgttgt ctgagtatgc ctttaaatct agatgtgtat tgaaatccat
     1861 tttaccgaat tcaatagctg cacgatctag gttaaaaacc atctcttcta attgaaccat
     1921 aaacttatct tttttcttac taaacattta aaatcctcca tttaagcgat tgtcaccaat
     1981 cacattcagt tataatttgt ttcaaattaa gacaagtgaa tttacaaact aatgatacaa
     2041 atttgttatt atcaatcgtc agtatatttt tagtgtactg atattaattt caaaaatgcc
     2101 tcacagtaaa caatttactg tatttgccct tatatgaaaa tagcacaaaa tttacaatcc
     2161 cacaattcaa tttacaaatt tttaacaatt ccgaaactta acttttacat aaaatttaaa
     2221 tagtggtagg catttgtttt gaaaaacttg atatataggc aattttttat ctacaaaact
     2281 ctaataattg taaatggaca tactcggaaa atttgcaacg atacaatatt tattacataa
     2341 c
//
LOCUS       DATTQF010000215         2328 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.219, whole genome shotgun sequence.
ACCESSION   DATTQF010000215 DATTQF010000000
VERSION     DATTQF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.219"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            69..920
                     /locus_tag="VB241_002632"
     CDS             69..920
                     /locus_tag="VB241_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP1245847.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMTLNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            895..1062
                     /locus_tag="VB241_002633"
     CDS             895..1062
                     /locus_tag="VB241_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245848.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            1055..2263
                     /locus_tag="VB241_002634"
     CDS             1055..2263
                     /locus_tag="VB241_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP1245849.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
BASE COUNT          806 a          293 c          385 g          844 t
ORIGIN      
        1 aattattata cttttaagtt agacatttcc gatatataat cttaataata gaaaaatagg
       61 tgatgataat gggagaatat attgttacta aaacattgaa caacaatgtc gtagtatgta
      121 ctaataatga tcaagaagtt attttaatcg gtaaaggtat tggttttaac aaaaaagagg
      181 gaatgacgtt aaacgaccaa actattacaa tagagaaaat ttataaatta gagagtgaac
      241 aacaaaaagc acattataaa agtttagttg aaatcgctga tgataatgta ttacaagtaa
      301 ttattgattc gttgaatttt atttctaata ctgcgatgaa tgttgattca aaacaacttg
      361 tagtttcatt aacggatcat attatatttg cttataaacg cttaaaacaa aatcaagtta
      421 ttagtaatcc atttgttatg gaaactatgc agttatatag tgatgcatat catattgcta
      481 aacaggtaat tgatcagtta aatgcagcat tagatgtgca ttttcctgaa gatgagatag
      541 gatttattgc attacatatt gcatctaaca cagaagattt atctatgcat gagatgacct
      601 tgatcaataa tgttattaaa aaaggtatag atatcattga atcagacctt gtgacaactg
      661 ttgataagga atcattacaa taccaacgtt ttataagaca tgtacaattt ttaattcgcc
      721 gattaagaag aaaagaatat atacatgcac aagatgattt tgtgtctatg attaaaaatc
      781 actatccgat ttgctataac acagcatata aaattttaac tatgatacaa aaacaatttg
      841 atgttaatat cagtgagtct gaaattatat atttaacatt acacattcat cattttgaag
      901 aaaggattaa tcaatcctaa attaagtgta ttacacgggc gtttcgttac tgtaaagggt
      961 atttgtatgg tatcattgat gttagtaatt ttacataagt ggactgtttt tatacaacat
     1021 cttatgaata ttttaagtgg tggtgaatct ataattgaat gaaaatgaaa agaatataag
     1081 aaagaacttt ttaaaattta ccgaatcacg gtatatgaag tttgttggtg ggaatgattt
     1141 agtcttctca ttaatagcac tagtattgtt gggtattgtt atttttattt tcgaaaaagt
     1201 atcatacgtt tttgatcctt ttatcatcgt ttttaagacg atagcagcac ctatcatcgt
     1261 ctctttaatt ctattctatc tatttaaccc aatcgtaaat atgatggagc gttatagaat
     1321 accaagagtt gcaggtattt ctattattta tctagctgta gtaggtgtta ttacgttaat
     1381 tgttaattta ttgataccta ttattggttc gcaagtagat agtttagtta aaaattcacc
     1441 gcaatatcta gaaaaattaa ttaattctat tgataaaata gcaaataata cgtttttctc
     1501 ttcgtattat agtcaaatta atgattggtt aaattcttta cctaagaaaa taccatctat
     1561 gttaagtgaa tttacagatg gctttgggtc taaaattgca acgtttgcag aaacgattgc
     1621 taatattggc gttgtgattg tcacaacacc atttgtacta ttctttatgc ttaaagatgg
     1681 acatcacttc aaagaatttt caacgaatat tatgccaccg aaattccgaa aagattttca
     1741 tgatctactt gaaaaaatga gtgttcaagt tggttcatac attcaaggac aaattatcgt
     1801 ttcattctgt atcggtatac tgttgtttat cggttattcg gttatcgggt tgaaatatag
     1861 cttagtatta gctagtattg ctgcagttac aagtgttgtg ccatatttag ggcctactat
     1921 agcgatttca ccagctattg tcatagctgc tataacatcg ccgtggatgc tcttaaaatt
     1981 agcagtagta tggactttag tacaatttgt tgaagggcac ttcatttcac caaatatcat
     2041 gggtaaaaca cttaagattc atccacttac aatcattttc attttactgt gtgcaggcaa
     2101 attgcttggt attgtaggcg ttattttagg tattccggga tatgctattt taaaagtatt
     2161 agttactcat ttattccaat tatttaaacg tcgatacaat cgtttctatg gtaatgatgt
     2221 aggtgaatat gatattaaag aaagtaataa aatagttgaa taagtaatta aaaataccaa
     2281 tagactatac gtttgttggt atttttatgg tgcttataat gttaggtg
//
LOCUS       DATTQF010000216         2291 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.220, whole genome shotgun sequence.
ACCESSION   DATTQF010000216 DATTQF010000000
VERSION     DATTQF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.220"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            988..1857
                     /locus_tag="VB241_002635"
     CDS             988..1857
                     /locus_tag="VB241_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEP1245850.1"
                     /translation="MEMSRLNLTDNNDINEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(1928..2290)
                     /gene="mscL"
                     /locus_tag="VB241_002636"
     CDS             complement(1928..2290)
                     /gene="mscL"
                     /locus_tag="VB241_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP1245851.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          941 a          299 c          372 g          679 t
ORIGIN      
        1 tatctcaata ttgaacgctt gagaactgag ggtgaacaat tagctgatga attaaataat
       61 ctaattgaag gtttagaaaa ggtcgaagat tcaataggta ataacgaaag tgattacgag
      121 aaaattatcg aactaaataa tgcgataact aacataaata atgaaattaa tgtaattaaa
      181 gaaaatgaaa aagctaaagc tgaattagat aaactattag gtagtaagca agagttagag
      241 aatcaaatta atgaagaaac atctacattg aagaatctcg aaataaaatt agatcgctac
      301 gataaatcaa aattggactt aaatgataaa gaaagtttta taagtgaaat taaatctgct
      361 gtaaagattg gagatcaatg tccgatatgt ggtaatgaaa ttcaggattt agggcatcat
      421 attgattttg acagtatcgc taaacgtcaa aatgaaatta aagaaattga agcaaatatc
      481 cacactatga aatcgaatat tgctgtacat aattctgaaa ttaaatttgt taatgaaaaa
      541 atatcgaata ttaatattaa aacgcaaagt gatttctcac tcgaagtatt gaataagcgt
      601 ctgctagaaa atgaaaatgc attgaataat caaagagagc ttaataaatt tatagaacaa
      661 atgaaagaag aaaaagataa tttaacgttg caaattcata ataaacaatt gcgtctaaat
      721 aaaaatgaat ctgagttgaa attatgtcga aatctcatca ctgaatttga aacactctcg
      781 aaatataata atatcactaa ttttgaggtg gattataaga agtatgtaca agatgtgaat
      841 caacatcaag aacactcaaa ccagattgaa gataagttaa tacaattatc tcaaagaaag
      901 ttaattgagc aaaataatct aaatcactat gaaaaacaac tagaaactta caataatgac
      961 ttagaattga atgaacaatc tattgaaatg gaaatgtcga ggctgaattt aactgataac
     1021 aatgatataa atgaaataat agcctggagg ggcgagcaag aggaattaga gcagaaaagg
     1081 gatacttata aaaaacgtta tcatgaattt gaaatggaaa tagctaggtt agaatcatta
     1141 accaaagata aagagttatt ggactctgat aaattaaaag atgaatatga actaaaaaaa
     1201 ggaaagatga atacactgat agatgaatac tctgctgttc attatcaatg tcaaaataat
     1261 attaataaaa cacaatctat agtttcgcat attaattact taaatcaaga attaaaggat
     1321 caacaagaaa tatttcaatt ggctgagatt gtcagtggta agaataacaa aaatcttaca
     1381 ttggaaaact tcgtcttaat ttactattta gatcaaatta ttgctcaagc aaatctgaga
     1441 ttagcgacaa tgtcagataa tcgataccaa ctaattaggc gagaagcggt ttctcatggt
     1501 cttagtggcc tagaaattga tgtatttgat ttgcattcaa ataagtctag acatattagc
     1561 tcgttatcag gtggagaaac tttccaatcg tcgcttgcat tagctttagg gttaagtgaa
     1621 attgtacagc agcaatcagg aggtatttca ctagaatcaa tttttattga tgaaggattc
     1681 ggtacattag atcaagaaac gcttgaaaca gcgttagaca ctttgttaaa tcttaaatca
     1741 actggtagaa tggttgggat tatttcacat gtgagcgaat tgaaaaatag aataccttta
     1801 gttttagaag tgaaatcaga tcaatatcag agttcaacaa gattcaaaag aaattaaaac
     1861 ataaaaaata gagatggtac tttggaatgt atagaacaac cttagcccat ctctttttat
     1921 ttaaaaatta ttttttctca cgtaataaat ctctgatttc agttaataac acaacatttt
     1981 cttccacaac tgcttcttct tcggcttctt ctttcttcat taaggtattt gcaatcttaa
     2041 cgaagatgaa taaagcaaac gcgatgataa taaagtcgat aacagattgg ataaataaac
     2101 catatttaat accccagaat gaccattctt tagcaaaatc aactgatccg aaaattttac
     2161 caattaatgg cataatgata ttttctacta atgaagatat aatcttgttg aaagctgcac
     2221 ccatcacaac agcaattgct aaatctaaga cgttaccttt taaggcgaac tctttgaatt
     2281 cttttaacat t
//
LOCUS       DATTQF010000217         2244 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.221, whole genome shotgun sequence.
ACCESSION   DATTQF010000217 DATTQF010000000
VERSION     DATTQF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.221"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            36..386
                     /gene="sarV"
                     /locus_tag="VB241_002637"
     CDS             36..386
                     /gene="sarV"
                     /locus_tag="VB241_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HEP1245852.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(488..1699)
                     /locus_tag="VB241_002638"
     CDS             complement(488..1699)
                     /locus_tag="VB241_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245853.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGVIISLFIKVDFEVTNSKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFAIERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1692..2132)
                     /locus_tag="VB241_002639"
     CDS             complement(1692..2132)
                     /locus_tag="VB241_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEP1245854.1"
                     /translation="MLSQEFFNSFITIYRPYLKLTEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
BASE COUNT          834 a          335 c          382 g          693 t
ORIGIN      
        1 aaacatctaa gaagaaacat aggaggcatc atattatgag taataaagtt caacgtttta
       61 tagaagcaga aagggagtta agtcagttaa agcactggtt aaaaacaaca cataagattt
      121 caattgaaga atttgtagta ctttttaaag tgtatgaagc tgaaaagatt agcggtaaag
      181 aattgaggga tacattacat tttgaaatgc tatgggatac aagtaaaatc gatgtgatta
      241 tccgtaaaat ctataaaaaa gagcttattt ctaaattgcg ttctgaaacg gatgaaagac
      301 aagtattcta tttctatagt acttctcaaa agaaattgtt agataaaatt actaaagaaa
      361 tagaagtgtt aagcgttaca aactaaaaac ttaaaaagca tgccaatcag tacatcataa
      421 ttgcgtcttg gggacagaca aatgatgaat agagattggc atgcttttta tttttgaata
      481 taaatattta gttcatggca tttctagtta catgacgtcc atgaattaag aagtaaacaa
      541 gcatagtaat gattgctaaa gcggccataa aaccgaagat ttcactatat gaaaacatat
      601 gagtaaataa cccaaggaat gatggaccga agccgacacc tgcatctaga ccaacgtaaa
      661 aagtagatgt cgcgatacca tatttaatcg ggggtgagac ttttatcgca atagattgca
      721 ttgcagatga taaatttcca taccctaaac ctaggcaagc accagcaagt aatattaacc
      781 agctttgata gcttgaaatt aagcacacaa atgaaaggaa aagcatgata aatgctgggt
      841 agacaataat attttcattt ttatcatcca tcaatctacc agcaataggt ctagtaatta
      901 acgatgctat agcatagcaa ataaagaaat agcttgctgc agttactagg tgtcgctcta
      961 tagcaaatgc ttgtaaataa gttaggatgg acgcataggt aacgccaatt aaaagcataa
     1021 taacagcaac aggaatggcc tcttttgcaa taaattgatg aatactaaat cttggtttat
     1081 caatgacatt agtttcagtt ttgctatttg ttacttcgaa atcaactttt ataaataatg
     1141 agataatgac tccgagtatg cctaatataa cacaaataat aaacagtaaa tcaattgcgt
     1201 attttgtaat aagtaacatg cctagaaatg ggccaatcgc tgtacctaat actaaactta
     1261 aagaaaataa actgatgcct tcactttttc tattaacagg ggtaacgtat gccgcaatag
     1321 tacctgttgc agttgtcaca actgcagttg cgataccgtt tatgagacgt acaaagatta
     1381 aaaaagctaa agatccatca ataaaataaa gtaattgcgt gataattaaa gcaattaaac
     1441 caataaataa taatcgttta ggtccgattt gatttacaaa tttacctgta gcaaatcgac
     1501 caattaaaga accgacaata aatagtccta caactaatcc ggctaatgaa tcggaagcgt
     1561 cgaaagcttg cttactatag ccagcaataa ctacaagtaa caaatacatg cataggtata
     1621 cgataaaatt gacaaaaaag ttaattgtaa aacttttggt gaaaataggt gatttattta
     1681 atgagccttg attagtcatt gtgtgcctcc tttaataata tctcatgaat ttgattcatc
     1741 acatttatgg tttcttgcat ttgatcttgt gaaatgtttg ttttggcaac tactgcttgt
     1801 tggagctttt gtacatcaag acaaacaatc tcatataatt catgcccttt aggtgttaaa
     1861 gttaaaaact tttgtcgttt atcctctaaa ctgttttcta ctgttataag gtcattttct
     1921 attagagctt ttaaagtttt tcttgcagta ggcttttcaa ttgcacgtct atgtgaaatt
     1981 tcaatgagag tagtgggttg atgtttagcg atatcgcgta agattaacca ttgaccataa
     2041 tatatattgt gtttttccaa aataggctct gttaatttta aatacgggcg atatattgtt
     2101 ataaaactat tgaaaaattc ttgtgatagc atagcgacat ctcctaagac aaaatagtta
     2161 gcttagctaa ccttttttac aacaatagta attataaaac ggggacaatt agaaatcaat
     2221 atataattat taagagcaaa aata
//
LOCUS       DATTQF010000218         2234 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.222, whole genome shotgun sequence.
ACCESSION   DATTQF010000218 DATTQF010000000
VERSION     DATTQF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.222"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            409..1538
                     /locus_tag="VB241_002640"
     CDS             join(409..655,655..1538)
                     /locus_tag="VB241_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558209.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP1245855.1"
                     /translation="MTRERRLFSSEFKLQMVRLYENGKPRNEIIREYDLTPSPLGKWI
                     KQYQNTGTFNHQDNLSDEEKELIKLRKEVQHLKMENDIFKASSVDHGTKIEVIQKNAH
                     QYSVSAMGNVLKILRSTYYDSIKRKDNKITKDDSNVERAVINIFNANRKVFATRRIKN
                     HLNDKGHTVSRRKIGRIMKKYNLVSVYTKAKYKNHPKETNEKLIKNHLNRAFNREQPM
                     ETLVSDLTYVKVAGTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISRINHNLEQI
                     KLFHTDRGKEFDNHLIDEVLETFKIKRSLSTKGCPYDNAVAEATMKAMKTEFVKQMQF
                     ENLEQLKTELFDYVNWYNNFRPHSSLQYLTPVAFKNLHMKTV"
     gene            1726..>2234
                     /locus_tag="VB241_002641"
     CDS             1726..>2234
                     /locus_tag="VB241_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245856.1"
                     /translation="MDNSFLESLKEILEPYDDIESNKDGFVFGGYIDWPSFVDYEDFQ
                     VEEVMSLLGLDIPFGSSKNPFEFYEILKVIQNIEKGLYEAICDEAIIIKEDEEISTED
                     FIDSIMDYKVQIPTVDRLKSSITELFEKYEIPQGIQYEYDEGMPESSKYWSLEMDEDL
                     YFIKEINFN"
BASE COUNT          926 a          258 c          387 g          663 t
ORIGIN      
        1 gagtaataac aaaaaaataa agttaggatc atttttaaca attataaatg tattgtcaca
       61 taaaccatac agagcaatag atgtaattgg ttcagcaaaa tttttgatga ggtatattga
      121 agatgtagat aaagctcatt atgatgctat gaaggattga attaatggtc gtataatttt
      181 ttatttgaaa agaacttaaa attcatggag aacataaatt gaactattat aagtatgctg
      241 aaaataaaat ttagaagaaa tagaaactta ttgatacacg aagcagattg tagtaactta
      301 tcacctatgt ttactacagt gagaagatta gctccaaata gaggtagaat aatgaattat
      361 ttataagctt cgacagaagc gtaaatacct gtggaatttc atttgattat gacaagagaa
      421 agaagattat ttagttcaga gtttaagtta caaatggtta gattatatga aaatggtaag
      481 cctaggaatg aaattatacg cgagtatgat ttaacacctt cgccgttagg aaaatggata
      541 aagcaatatc aaaacacggg tacattcaat caccaagata acttatcaga tgaagaaaaa
      601 gagctgatta aattacgtaa agaagttcaa catttaaaaa tggagaacga tattttaaag
      661 caagcagcgt tgatcatggg acgaaaatag aagtcattca aaagaatgca catcaatatt
      721 cagtatcagc aatgggtaac gtcttaaaaa tactaagaag tacctattat gattctataa
      781 aaagaaaaga taataaaatc actaaagatg attcaaacgt agaacgtgcc gtcataaata
      841 tttttaatgc taatagaaaa gtctttgcta caagacgaat taaaaatcat ttaaatgaca
      901 agggtcatac tgtatcaaga cgaaagatag gtcgtatcat gaaaaaatat aatctagttt
      961 ctgtttatac gaaagctaaa tacaaaaatc atccaaaaga aactaatgaa aaactaatta
     1021 aaaatcattt gaatcgcgct tttaatagag aacaaccaat ggagacatta gtaagtgatt
     1081 tgacatatgt aaaagtcgca ggaacatggc attacatatg tttatttata gatttattta
     1141 atagagaaat tgttggttac agtgcaggta aaaataagga cgcaaattta gtatcaaaag
     1201 caatcagcag aataaatcat aatcttgaac aaatcaaact attccacact gatagaggca
     1261 aagaatttga taatcatttg atagatgaag tcctagaaac atttaaaatc aaacgttcat
     1321 taagtaccaa aggttgtcct tatgataacg cagttgcgga agcaacgatg aaagcaatga
     1381 aaaccgaatt tgtaaaacaa atgcaatttg aaaacttaga acagttaaaa acagaattat
     1441 ttgattatgt aaattggtac aacaatttta gaccacattc ttcattacag tatttgacgc
     1501 cagtggcatt taaaaatcta cacatgaaaa ctgtctaaaa aaatgttgac ataccaatga
     1561 aatcttgaag gatagctttt catttagagt aaaagaaatt gcaacgatta ttctcaatga
     1621 gatagcatat tctgttaaca gatatcatgc tcaaagatta attaaagata ttatatctat
     1681 tggaattaaa ccaataattg aggatatttt aaaaggagat aaataatgga caatagcttt
     1741 ttagaaagcc taaaagagat tttagaacca tatgatgata tagaatcaaa taaagatggt
     1801 tttgtgtttg gtggatatat agattggcct agtttcgttg attacgaaga tttccaagtt
     1861 gaagaagtaa tgagtttatt agggttggac ataccatttg ggagttctaa aaatccgttt
     1921 gaattttatg aaattttaaa ggttatacaa aatattgaaa aaggattata cgaagctatt
     1981 tgtgatgaag caattattat aaaggaagat gaagaaatta gtacagagga ttttatagat
     2041 tcaattatgg attataaggt tcaaatccct acagtggata ggttgaagag ttcaataaca
     2101 gaattatttg aaaagtacga aattcctcaa ggtatacaat acgagtatga tgaaggaatg
     2161 ccagaatcat ctaaatactg gtcactagaa atggatgaag acctttattt tattaaagaa
     2221 ataaatttta atga
//
LOCUS       DATTQF010000219         2229 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.223, whole genome shotgun sequence.
ACCESSION   DATTQF010000219 DATTQF010000000
VERSION     DATTQF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.223"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            145..2214
                     /gene="eap"
                     /locus_tag="VB241_002642"
                     /gene_synonym="map"
     CDS             145..2214
                     /gene="eap"
                     /locus_tag="VB241_002642"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HEP1245857.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKQIDKSSSSLHH
                     GYSKIQIPYTITVNGTSQNILSSLTFNKNQQISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAKYTVHFKNGTKRVVDLKAGIHTADLINTSDIKAISVNVDTKKQVKDKEAKANVQ
                     VPYTITVNGTSQNILSNLTFKKNQQISYKDLENNVKSVLKSNRGITDVDLRLSKQAKF
                     TVNFKNGTKKVIDLKAGIYTANLINTGDIKNININVETKKQAKDKEAKANNQVPYSIN
                     LNGTTTNIQSNLAFSNKPWTNYKNLTAKVKSVLKSDRGVSERDLKHAKKAYYTVYFKN
                     GGKRVIHLNSNIYTANLVHAKDIKKIEVTVKTGSKANAERYVPYTIAVNGTSTPNLSD
                     LKFKGDSRVSYSDITKKVKSVLKYDRGIGERELKYAKKATYTVHFKNGTKKVINLNSK
                     ISQLNLLFVKDIKKIDVDVKTGSKAKADSYVPYTIAVNGTSTPIASKLKLSNKQLIGY
                     QDLNKKVKSVLKHDRGINDIELKFAKQAKYTVHFKNGKTQVVDLKSDIFTRNLFSVKD
                     IKKIDIDVKQHTKSNKALNKVANIATKVKFPVTINGFSNVVSNEFAFLHPHKITTNDL
                     NAKLRLALASDQGITKHDIGLSERTVYKVYFKDGSSKFVDLKAAKQDSKVFKATDIKK
                     VDIEIKF"
BASE COUNT          963 a          290 c          343 g          633 t
ORIGIN      
        1 caaaattaaa agaactttta acagtgttaa aaaattattg ttaaagataa tttattatta
       61 atattcagtt aattcaaaaa atagagaaag tctggctata attaagttgc aatcacgaat
      121 tacataaaaa aggagagata atttatgaaa tttaagtcat tgattacaac aacattagca
      181 ttaggtgttt tagcatcaac aggagcaaac tttaatacta acgaagcatc tgccgcagct
      241 aagcaaatag ataaatcatc aagttcatta catcatggat attctaaaat acagattcca
      301 tatacaatca ctgtgaatgg tacaagccaa aacattttat caagcttaac atttaataag
      361 aatcaacaaa ttagttataa agatatagag aataaagtta aatcagtttt gtactttaat
      421 agaggtatta gtgatatcga tttaagactt tctaagcaag caaaatacac ggttcatttt
      481 aagaatggca caaaaagagt tgtcgatttg aaagcaggca ttcacacagc tgacttaatt
      541 aatacaagtg acattaaagc aattagtgtt aacgtagata ctaaaaagca agtgaaagat
      601 aaagaggcaa aagcaaatgt tcaagtgccg tatacaatca ctgtgaatgg tacaagccaa
      661 aacattttat caaacttaac atttaaaaag aatcaacaaa ttagttataa agatttagag
      721 aataatgtaa aatcagtttt aaaatcaaac agaggtataa ctgatgtaga tttaagactt
      781 tcaaaacaag cgaaatttac agttaatttt aaaaatggca cgaaaaaagt tatcgatttg
      841 aaagcaggca tttatacagc gaacttaatc aatacaggcg atattaaaaa tatcaatata
      901 aatgtagaaa ctaaaaagca agcgaaagat aaagaagcaa aagcaaataa tcaagtgcca
      961 tattcaatta atttaaatgg tacaacaact aatattcaat ctaatttagc attttcaaat
     1021 aaaccttgga caaattacaa aaatttaaca gcaaaggtta aatcagtatt gaaatctgac
     1081 agaggcgtta gtgaacgtga tttgaaacac gcaaagaaag cgtattacac tgtttacttt
     1141 aaaaatggtg gtaaaagagt gatacattta aactcgaata tttatacagc taatttagtt
     1201 catgcgaaag atattaagaa aattgaagtt acagttaaaa ctggatcaaa agcgaatgca
     1261 gagcgttatg taccatacac aattgcagta aatgggacat caacaccaaa tttatctgat
     1321 ttaaaattta aaggtgactc acgtgtaagt tacagtgata tcactaaaaa agttaaatca
     1381 gtattgaagt atgatagagg tatcggtgaa cgtgaattaa aatatgctaa aaaagcaact
     1441 tatacagtac attttaaaaa tggaacaaaa aaagtgatta atttaaactc aaaaattagc
     1501 caactgaatt tgctttttgt caaagatatt aaaaagatag atgtcgatgt taaaacaggg
     1561 tcaaaagcga aagcggatag ctatgtacca tacacaatcg cagtaaatgg gacatcaaca
     1621 cctattgcat caaaactaaa actttcgaat aaacaattaa ttggttatca agatttaaat
     1681 aaaaaagtta aatcagtttt aaaacatgat agaggtatca atgatattga attgaaattt
     1741 gcgaaacaag caaagtatac tgtacacttt aaaaatggaa agacacaagt agttgacctt
     1801 aaatcagata tctttacaag aaacttattc agtgtcaaag atattaaaaa gatagatatt
     1861 gatgtaaaac aacacactaa atctaataaa gcgcttaata aagtggctaa catagcgact
     1921 aaagtgaagt ttccagtaac gataaatgga ttttcaaatg tagtttcaaa tgaatttgcg
     1981 tttttacatc cacataaaat aacaacaaac gacttgaatg ctaaacttag actagcgtta
     2041 gcaagcgatc aaggtattac taaacatgat attggacttt ctgaacgcac tgtgtataaa
     2101 gtgtatttta aagacggatc atcaaaattt gttgacttaa aagctgcgaa acaagattca
     2161 aaagtattta aagcaactga cattaaaaaa gtagacattg aaattaaatt ttaatcttta
     2221 attttagat
//
LOCUS       DATTQF010000220         2155 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.224, whole genome shotgun sequence.
ACCESSION   DATTQF010000220 DATTQF010000000
VERSION     DATTQF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.224"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            162..2102
                     /gene="ltaS"
                     /locus_tag="VB241_002643"
     CDS             162..2102
                     /gene="ltaS"
                     /locus_tag="VB241_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HEP1245858.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT          802 a          314 c          340 g          699 t
ORIGIN      
        1 tacaaataat gaaaattata aacttgaatc tgaaagtaat tactataatt atgacaatgt
       61 taacttttaa acgcacttat taattaacta cataatgtta atatctaatt tattcaagta
      121 ctttcgcaag atttattatc taaataacgg gggaaagaat catgagttca caaaaaaaga
      181 aaattagtct ttttgcgttc ttcttattaa ccgtaataac gattaccttg aagacgtatt
      241 tttcttatta tgttgatttt tctttaggtg ttaaaggttt agtacaaaac ttaatattat
      301 tgatgaatcc ttatagttta gtagcactgg ttttaagtgt gttcctattc tttaaaggca
      361 aaaaagcatt ttggtttatg ttcataggcg gcttcttatt gacgttccta ttatatgcca
      421 atgttgtgta ctttagattc ttctcggatt ttttaacgtt tagtacttta aaccaagtag
      481 gtaacgtaga atctatgggt ggtgcggtta gtgcatcatt caaatggtat gactttgttt
      541 atttcattga tacgttagtt tacttattca ttttaatatt taaaacaaaa tggttagaca
      601 caaaagcatt tagtaagaaa tttgttcctg tcgtaatggc ggcttcagta gcattattct
      661 tcttaaactt agcttttgct gaaactgaca gaccagaatt attaacacgt acatttgacc
      721 ataaatattt agtgaaatat ttaggaccgt ataactttac agtatacgat ggtgttaaaa
      781 ctatcgaaaa taatcaacaa aaagcgttag catctgaaga tgacttaaca aaagtattaa
      841 attatacgaa acaacgtcaa acagagccta acccagaata ttatggggta gcaaagaaga
      901 aaaatattat taagattcac ttagaaagtt tccaaacctt cttaattaat aaaaaggtta
      961 atggtaaaga agtaacaccg tttttaaaca aattatcaag tgggaaagag caattcacat
     1021 acttccctaa ctttttccat caaacaggtc aaggtaaaac atctgactct gaatttacaa
     1081 tggataacag tttatacggt ttaccgcaag gttctgcctt ttcattaaaa ggagataata
     1141 cgtatcagtc attaccagca attttagatc aaaagcaagg ctacaaatct gatgtcatgc
     1201 acggtgacta taaaacattc tggaacagag accaagtata taaacacttt ggtatcgata
     1261 aattctatga tgcaacatac tatgacatgt cagataaaaa cgttgtaaac ttaggcttga
     1321 aagacaaaat tttctttaaa gattctgcta attatcaagc taagatgaaa tcaccattct
     1381 attctcattt aattacattg actaaccact atccattcac attagatgaa aaggatgcaa
     1441 ctattgagaa gtcaaacaca ggtgatgcaa cagttgatgg ttatattcaa acagcacgtt
     1501 atttagacga agcattagaa gaatatatta atgacttgaa gaaaaaagga ttatatgaca
     1561 attcagtgat tatgatttat ggtgaccact atggtatctc tgaaaaccat aacaatgcca
     1621 tggaaaaact attaggtgaa aaaatcacac cagctaaatt tacagattta aacagaactg
     1681 gtttctggat taaaatccct ggtaaatctg gtggtatcaa taatgaatat gctggtcaag
     1741 tcgatgtaat gccaacaatt ttacatttgg ctggtataga tacgaagaac tatttaatgt
     1801 tcggtactga tttattctct aaaggtcata atcaagtagt tccattcaga aatggtgact
     1861 ttataacaaa agattataaa tatgttaatg gtaagattta ttctaataaa aataatgaac
     1921 tcataactac tcaaccagct gatttcgaaa agaataaaaa gcaagttgaa aaggatctcg
     1981 aaatgagtga caacgtgctt aatggtgatt tatttagatt ctacaaaaat ccagacttca
     2041 aaaaggtaaa tccttcgaag tataaatatg aaacaggacc taaagcaaac tctaaaaaat
     2101 aatatttaaa catgaacccg gattgataaa atatcaatcc gggtttttat ttatg
//
LOCUS       DATTQF010000221         2131 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.225, whole genome shotgun sequence.
ACCESSION   DATTQF010000221 DATTQF010000000
VERSION     DATTQF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.225"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..418
                     /locus_tag="VB241_002644"
     CDS             <1..418
                     /locus_tag="VB241_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245859.1"
                     /translation="INKENQKNGNKRREQQESMNGNDVKSASVESNANNYSKQPQKAS
                     QQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAYDTDVQKRQIQNAT
                     QNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            408..1454
                     /locus_tag="VB241_002645"
     CDS             408..1454
                     /locus_tag="VB241_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP1245860.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFTFPVIVMF
                     LLAPDSNKDKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            1461..2051
                     /locus_tag="VB241_002646"
     CDS             1461..2051
                     /locus_tag="VB241_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245861.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
BASE COUNT          832 a          258 c          417 g          624 t
ORIGIN      
        1 tataaataaa gaaaatcaaa aaaatggtaa taagcgaaga gaacaacaag agtcaatgaa
       61 tggtaacgat gtaaaatctg cttctgttga atcgaatgca aataattatt ctaaacaacc
      121 acaaaaagca tcacagcaag aacatcaagt acgagaaaca agacagcgaa aagatataca
      181 aaggtcgcca caagtggtta atcagccatt aaataatgag aatcattcaa taaatcgtaa
      241 agaacagaag tcagttcaaa ctgcttatga tactgatgtt caaaaacgac aaatacaaaa
      301 tgcgacacag aatcaacaaa gtagacaatc tggaaatcgt aatcaaccta ttacacgtaa
      361 ttctcagtca aaagatcgtc taaaagagca aaaggatatc aataagcatg gtaaataaaa
      421 aagatattgc taaaaaagta ataggtaaaa cgccaatagg tgttaaattg aaattagcga
      481 aagtcatcat cattttgtgt gtggtggctt tttttacatt tccagtaatt gtaatgtttt
      541 tgttagcgcc tgattcaaat aaagataaag atgatgctgg ttgtactgtt agtggcggta
      601 atgtttcggc aaatggtatt gataagttta atgaaaatgc taaaggtgga aaattagagg
      661 gtaaaggaaa agaaatacag aaaattgcag aaaaaaataa agtacctgtg aatattttta
      721 tggctattat tgcgagtgaa tcacaatggg gtaaaggaga aaatgcgaca agacaaaata
      781 atcctttgtc tgttatgggt tcaaaatcga ttcatgattc aacatatcca actattgaag
      841 acggtcttaa tgcgggagct aaaaatttgt atgatgttta tatttctaaa ggtttagata
      901 ctcctaaaaa aataggtccc aaatatgcgc ctgttggtgc gtcaaatgac cctaataata
      961 tgaatgcccg ttggatacca acagttgaaa aaattatgaa agatttaggc ggttcagaag
     1021 ctaaaacgtc atgtagtaat ggtaaaggaa agtctataaa gtttaatggt aaattgccac
     1081 attggtcaaa tgatgaccct ggaaaaggta atttatacac tgctggtcaa tgtacttggt
     1141 atgcatacgg tatgcgtcaa aaaatgggta aacctgtatc aacttattgg catgatgcac
     1201 ataaatggaa tgatagggct aaggctgagg gttataaagt tgataaaaat ccggaacctg
     1261 gtgcattatt tattgctgaa caaggtgcag gcggtcatga tgggcattat ggtcatgtgg
     1321 ctgttgttat tggtgtaagt gatggtggta aaacatttag aataacagaa atgaattggg
     1381 aaggtgcttt taaagttaat gaacgtacat tgaaaatgac tgatggatat agttttattc
     1441 atgataagga gtgaaaataa atgaagcaaa aaattattat gtttactctt gtaacagtaa
     1501 tattattttg tgctgtttta attggttatc agattcctaa acaacaagtt aaaatgaaac
     1561 aaaatcaaat tgaagattta caggaagaac aacgtatatt aagagataaa aatggagagt
     1621 taaataaatt agtaaagaga caatcaaaaa cagttatttc tgatgaagaa aagcaaatac
     1681 gtgaagtttc ttcaaatttt gttaaacaga tgtttgaaat gaaaaaagat tcttctttta
     1741 aatcaaaagc accacaaata aagcctttag ttacaaagga ttattacgat acattattta
     1801 aggattctaa agataaatat gatttatatg atgatataac ggttaatgat attcatgttt
     1861 attttgatac ttatgatcct aaaaaagata gttataaagt ttttgttcag ttcgacgaac
     1921 gtattgaaac agatggagat gataaaattg aacatcgtca aacatctgca caattggatt
     1981 tagttcgtac agctgaaggt tggcgtattg ataatttaaa acgtttcaat ttaaaacctc
     2041 taggacgtta gtttgtttca agacgatagc aatgatataa tttacgtaaa aaggagtgaa
     2101 tttatcatga ggcgatggtt tgtattaatt t
//
LOCUS       DATTQF010000222         2111 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.226, whole genome shotgun sequence.
ACCESSION   DATTQF010000222 DATTQF010000000
VERSION     DATTQF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.226"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(148..705)
                     /gene="efp"
                     /locus_tag="VB241_002647"
     CDS             complement(148..705)
                     /gene="efp"
                     /locus_tag="VB241_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP1245862.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(731..1792)
                     /locus_tag="VB241_002648"
     CDS             complement(731..1792)
                     /locus_tag="VB241_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP1245863.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAADIVDETYEYILTVAKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYFNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1897..>2111
                     /locus_tag="VB241_002649"
     CDS             1897..>2111
                     /locus_tag="VB241_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245864.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTAS"
BASE COUNT          675 a          383 c          285 g          768 t
ORIGIN      
        1 tattttaaaa tttattttaa aaataaaaag cctcatatca gcatataacc tataatcatt
       61 ttaggcaata gctttatgag acttatttgg aatatcttac gttaaatcag tatagtgaat
      121 acaagctatt tgttaacaaa ttagagatta tcctcttgaa atgtagcttc catcaccagt
      181 gttgataatt aaaacgtcac cttcgtttac aaataaaggt acatttaatg tataacctgt
      241 ttcaacagtt gccgatttag tagcaccggt tgcagtatca cctttaatac caggttctgt
      301 ttcagttact gttaattcaa cagttttagg taattcaaca ccgatagttt caccttcgta
      361 tgtttgaatt tgtacttcca taccttcttt taagtaattc aattcttctt ttaagtaatc
      421 acttgaaagt tctgtttgtt caaagctttc attatccata aatacatgat tatctccgtc
      481 agcatataaa tattgcatgc gacgattttc aatcattgct ggttcaactt tttcaccagc
      541 tctaaacgtt ttctcttgaa ttgcaccagt tcttaaatta cgtaattttg aacgaacgaa
      601 tgctgaacct ttaccaggct ttacatgttg gaagtctata actttccaaa tagcgttatc
      661 aacagaaatt gttaaacctg ttttaaaatc attaaccgaa atcattcagt ttcctcctca
      721 ttttacacgc ttatgttaaa actataaggt cttttgtgca tttagtaaag acttgacaac
      781 cattttctgt aattaaaata tcatcttcta ttcttatacc gcccaaacct tctatataaa
      841 caccaggttc tactgtaaca cagttgttaa cttgaagttt atcttgtatc gtacgagcca
      901 gcattggccc ttcatggatt tctaaaccaa taccatgtcc tagtgaatgt ccaaattctt
      961 ttccataccc ttttgactct aaatagtttc ttgaaatggc atcagcttct gcaccagtca
     1021 tgccaggtct aatctcatta attgctttca tttgagattc aagtactatt tgatatattt
     1081 ctttcagttt aggatctggt tctccaatag caaatgttct agtaatatct gaacaatatc
     1141 cattgaaata agcaccaaaa tccaatgtta tcatgtcgcc tttttcaata attttgtcac
     1201 ttgcaacacc atgtggtaat gcacctctat gaccagatgc tacaatcgta tcgaatgatg
     1261 gtccatctgc tcctaattct agcattttac tttctaatat tgcctttaat tctttttcag
     1321 tcataccagc ttttgcaacc gttagtatgt attcatatgt ttcatcaacg atatctgctg
     1381 ctttttgaat caatgcaatt tcgtcagcat cttttacgtc tctaatttta tctacagtat
     1441 tagaaatgct tattaatgat atacggcttt tatttaattc aaggtatgta tcataactca
     1501 catggtgccc ctcaaagcct acattttcaa aattctcttg gtgtagcaat tctttaatct
     1561 caccaatgat agtagattta cgattaataa tttcataatt tggcgcctgc ttagttgctt
     1621 gatcaatata tctaaagtct gttatcaaat attgtttatc tttagatatg ataagtgctc
     1681 cactggtacc agtaaaacct gataaatatc ttctattgta atccgaaaga atgataatcg
     1741 catctaaatg tttttgttct aaaatacgat gcacttgtgt tattctgctc atcttattac
     1801 ctcctagtga aacgtttgtg catttcaact ttatacatta aaataatatc ataataagga
     1861 taaaaaataa tagatattga ttttagggag atagtaatga aaaaattggt ttcaattgtt
     1921 ggcgcaacat tattgttagc tggatgtgga tcacaaaatt tagcaccatt agaagaaaaa
     1981 acaacagatt taagagaaga taatcatcaa ctcaaactag atattcaaga acttaatcaa
     2041 caaattagtg attctaaatc taaaattaaa gggcttgaaa aggataaaga aaatagtaaa
     2101 aaaactgcat c
//
LOCUS       DATTQF010000223         2108 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.227, whole genome shotgun sequence.
ACCESSION   DATTQF010000223 DATTQF010000000
VERSION     DATTQF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.227"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            61..915
                     /locus_tag="VB241_002650"
     CDS             61..915
                     /locus_tag="VB241_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245865.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     NQITAIFK"
     gene            complement(1058..1591)
                     /locus_tag="VB241_002651"
     CDS             complement(1058..1591)
                     /locus_tag="VB241_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEP1245866.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHKGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            1730..1918
                     /locus_tag="VB241_002652"
     CDS             1730..1918
                     /locus_tag="VB241_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEP1245867.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(2031..>2108)
                     /locus_tag="VB241_002653"
     CDS             complement(2031..>2108)
                     /locus_tag="VB241_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEP1245868.1"
                     /translation="SVRIDKLFADNRFDAWKKANEKGWI"
BASE COUNT          728 a          326 c          278 g          776 t
ORIGIN      
        1 aatctttatt aattattatt gtatacaaag ttaaagaagt caaaaaggat tggtgaaaaa
       61 atgtcttttt atgttgtact tatcattatc attgtagcat taattggtat attagtttta
      121 aatcaaaggt atagtaatag taaaatagat accgaagttt atgcaagaaa gcaacttatt
      181 aaaaagaaca aagcattaag tgcagaaaat gctgaattaa gaagtcaaat gctgagttct
      241 aataatgatg ttggccacca tgcatacaaa aatgctaaaa gagaattacg caagatttta
      301 gacagctatc tcgaaaatgg taaattaaaa tattacgata ttatcgttac aagcaattta
      361 gctactaaac atccgttttt cgagtatgca cgttcatttg attttatcat tgtttcagat
      421 atcggtttaa taaacgttga tgtaaaaagt tggggtgaaa aaacatttta tcactttgat
      481 gtacctgatg agcatgatac agaaatgagc aatagtaata ttgaaaaagt tgtcggtcat
      541 tacattagtc agcaatatca tgatcagttt aattcatcaa gaaagtctat ctatactttt
      601 acagaaactg ttcaaccaaa tcgtgttatt tatgattttt atgattatga tccgtatcaa
      661 ttagcagcaa ataatgctaa agcattaaag gatcatattg aacaaaactt taactttaaa
      721 gtccaaagta ccggtgttat ttactttagt gatggtacag ttaatatcat tcaaggctct
      781 gaagaaagag ataaatatgt tgatacagtt tcaacaaaat cttcgttacg acgtattatt
      841 agcgaagcca tagagctatc taaacaccct ttaaataaag aacaggttaa tcaaatcaca
      901 gctatattca agtaaaatca aaggcataat aatccattac gctcgatacg cactatggtt
      961 attatgcctt taaattattt ctatatataa atttatggta ataataaatc gatgcttcaa
     1021 gtatatttta ctttaatata tataatcacc tatttaatta tttactccaa atatttaatt
     1081 tctgttgttt agctttattt tgtgcttcta taaatacatt tctatatttg ccatttggtg
     1141 aaaaatactt ttctctagca agtccctttt ccactaattc cttattgtac atacgatctt
     1201 tacttatcca tacatacgcc aatgttctac catagcgatc ttgtttttct ttatcatatt
     1261 ctaaataaac atcttgattt gttaatgtct tcttactata atttgatgct tctttgccaa
     1321 atggttgtac aggcgtattc ggtttcaccg tttctggcgt atcaacccct ataagcctaa
     1381 ctttaatttc tttaccattt tgatttgcaa taaatgtatc accatccaca actctttgaa
     1441 catgtacttt atctttacca tttaaatctt gtacagtttc atgatttgtt ccctttttaa
     1501 aaggaccctt atgattcata aattgaaatg ctaatacacc tacaataatg atggctacca
     1561 caatcatagc aagcgattta tttgacttca ttttgccacc tacttctctt ttttcattcc
     1621 gattaatcat ataaaagtta agaaatatcg tcaatagcca tatctacata tttcaatatt
     1681 ttggaaagct atagcatgat ataatgttat tgttaaggag tgaaaatgaa tgaacatttc
     1741 aaatgaaaat acagtcacat taattgcagt aattatcgca attatcattg gtattttcct
     1801 acaaatattt tttaaattac cattaatagt gacggcagta ctatctattt tattgggtat
     1861 ctttgtaggc tttatcgttt acctgattgt ttcgttttat aataaacgaa aaaattagtt
     1921 aacaaatcta acttgcgagt accaaaataa aagtattgag taagattttg attattgact
     1981 cactttcaaa gtctcttaaa tcaaaacact caatactttt ttgtttgtat ttagatccag
     2041 cctttttcat ttgccttttt ccaagcatca aaacgattat ctgcaaataa tttatcaatt
     2101 ctaacaga
//
LOCUS       DATTQF010000224         1961 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.229, whole genome shotgun sequence.
ACCESSION   DATTQF010000224 DATTQF010000000
VERSION     DATTQF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.229"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            104..364
                     /locus_tag="VB241_002654"
     CDS             104..364
                     /locus_tag="VB241_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245869.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            393..581
                     /locus_tag="VB241_002655"
     CDS             393..581
                     /locus_tag="VB241_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245870.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            769..1338
                     /locus_tag="VB241_002656"
     CDS             769..1338
                     /locus_tag="VB241_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245871.1"
                     /translation="MNYIKRTLILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIITKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAADWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1426..1710
                     /locus_tag="VB241_002657"
     CDS             1426..1710
                     /locus_tag="VB241_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245872.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1772..>1961
                     /locus_tag="VB241_002658"
     CDS             1772..>1961
                     /locus_tag="VB241_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245873.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEIDLDN
                     GNVSNIDHSLLVSVDPKAL"
BASE COUNT          693 a          280 c          279 g          709 t
ORIGIN      
        1 tacaaataat attttaaaaa taactatact ttttatttac ctattggaaa atagtgttat
       61 catatttatt aagaagctat ttttatacta gtaggtgtca gtcatgaata acattttgtt
      121 aaatgctatc aatatagtta ttactaccac ttttgttatc tttaatattt taatcacata
      181 taataaagat ttagatgatt tatgttggct cctgcctggt attatcattt gtggtgtgat
      241 actcatagta tcctttacca ttgcaatgat aactaaaaac tggttaagtg aaatattatt
      301 ttttataaat atcgtactcg ttctctatta catttatcct attttttata gttttatagg
      361 ttaataatta cggtatataa ggagggtttc acatgtcttt acattttgca attctgtttt
      421 ggctagcatt aattttctta gttgccgcta cgtttatact cgtattaatg aaaaaaactg
      481 gcaaagaatc taaaaaagag tcctatttaa gtttcactgt cattctctat attttcggat
      541 tcgctatttt aatatacaca tttatatttg gtgtgctata aatgaaattc attttatatc
      601 cagtctgaaa cgtctcaaag atgttttaga ctgtttttta ttcttaatag acatttcaca
      661 ctctcatact ttaatattta agatatgttt tagtcttgca tgcctattaa agattaaaat
      721 tataattcaa ggctatttac atatatttct tcaacaagga gcttttaagt gaactatatt
      781 aaacgaacgt taattttact tatattattc gttgtcgtat cccctataaa ctcaccaaaa
      841 acaattgcag ataataagta ttcagaaatt caagatgaca aattccaatt acaacctggt
      901 gatattatta ttacaaaagg ccctgtcatg tggggatttt ttggtcattg tagtatcgcg
      961 attgatgata aaacgatttt gcaaattgag gggcctggcg acaaaccaac tacacaatcc
     1021 ttcgaatctt ttaaatataa ttatgcaagt ggcaaaaatg attggatgaa agtttatcgt
     1081 tgcagctatc ctggtgcagg taaaaaagcg gctgactggg ttaagaaaaa ctatgaaaat
     1141 acaaatcatc gttatctagt tacattaaat ttgaacagca aaaaatttac gtattgtact
     1201 aaaattatct atcaagctta taaatttggt gtaagtgaaa aatcagttaa aagttatgga
     1261 ctacatatta tttcacccta tgcaattaaa gataatttta tagatccata taaactaaga
     1321 cttgttaaag cttattaaca cagtttcatc aaatagttaa attagtttgt gttttgcaat
     1381 aaattgggta tagattacaa atgatatttc aggaggccca atattttgaa tgaactaact
     1441 aaagaacaaa agtatacaat agctaaattt tacaaattat atattgaacg ttcgaataat
     1501 ggagaaacag aaacagtcgc taactttttt ggcgatgcta aagatgcacg tgaaaactat
     1561 ttttgcgatc gtgattatca agacttctta actaactgcc aaattttaat tcaaaacaaa
     1621 tacttaactg gtgaagtttt ggacgataac atttacaata tttctatttt aaacaagaca
     1681 tttattgaat ttgaacaaaa ttttggttga acatgaatgt ctcaactata aactttctaa
     1741 ccctaattta aagcaggagt gaaaaacata catgagtgaa tggcatatta ataatcaatc
     1801 tacaaaacct tttttaatac aacaagcgga aaagcaattg tctattgtta aaccgttgcc
     1861 taatggtagc ttccaaatac tcacagaaat agatttggat aatggcaatg ttagtaacat
     1921 cgatcattct ctacttgttt cagtagaccc caaagcatta g
//
LOCUS       DATTQF010000225         1921 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.230, whole genome shotgun sequence.
ACCESSION   DATTQF010000225 DATTQF010000000
VERSION     DATTQF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.230"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            43..753
                     /locus_tag="VB241_002659"
     CDS             43..753
                     /locus_tag="VB241_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEP1245874.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            737..1741
                     /locus_tag="VB241_002660"
     CDS             737..1741
                     /locus_tag="VB241_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEP1245875.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEQMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT          716 a          261 c          349 g          595 t
ORIGIN      
        1 aaaaatcaaa gtaaagtaac caaatattga agaggtgtac aaatgaaaat atatagtcaa
       61 ggtgaccaag ccattgtagt agcaattgaa aaagaagtat ctaaaagttt aaccgaagat
      121 ttattaacac ttcgctcata tttaattgaa caaaattatc catttattat agaaattgtg
      181 ccatcagaat cagacatgat gattgtctac gacgcaaggg atatgattaa acaccataat
      241 atacaatcac cttttttata catgaaagca ctaatagaat cgattcattt aaacataaaa
      301 catgatttta accagcaaga tttgattgaa ataccaattg tgtatggttc gaaatatggt
      361 ccggatttag aatcactttt aaaacattac aaaatcaagc tagaaacatt tattgaatta
      421 cattctaagg cgcaatattt tgtttcgatg atgggatatt cacctgggtt tccttattta
      481 actggattaa ataagaaatt gtatattaat cacacgagta aacagaaaaa attcattcca
      541 gctggttctg tagtacttga agggaaaaaa tgcggtattg taactacgga tacaattaat
      601 gattggttag ttattggtta tacaccatta tcacttttta atccgaaaga atcagatttc
      661 gcacgcttaa agttaggcga taatattaaa tttagaccta tcaatgaaaa tgaattagaa
      721 gtaggagcgt ttaaagatgt caatcataat tgaaaaaagt ggcttattca gtagctttca
      781 ggactttggt agaaggggat atgaacatga tggtgtaatt ccatgtggtg cacttgatac
      841 tttagcacat gaaattgcta atcgattagt tgcaaatgac aagaatgaag caactttgga
      901 aatgactaat aaaatggcaa cgattcgttt tacagaacct acgctgattg cattagcagg
      961 gggtaatgtc aaagcttaca ctgagcaaat gactatatct ccatataaat tgtatttgtt
     1021 agataaaggc gatgttttaa agtttagaga aacaagttat acatcgcgag tgtatttagc
     1081 tgtgggaggt ggatttgaat tagatgcatg gttaggatct aattcaaccg actttaatgt
     1141 aaaaattggt ggttttaaag gaagaacatt acaagatggc gatgaaataa agcttaagag
     1201 agattataca gctcgtcatc ataagttatt tgaaaacctt gctcacacga aacaaacaga
     1261 ttggggtatt gatgggtacg ccttgtcatt taattatatg tctgatgtat ttcatgtcgt
     1321 taaaaataaa ggtacggaag attttaaaga agatgccatt caaagatttg tgaaacatga
     1381 ttataaagta acgagcaaag caaatcgcat ggggatgatg cttgaaggtg aaaaaatcaa
     1441 agctttttat gaagatatgc caccgtatca gactgtcaaa aaaggaacga tacaaattaa
     1501 gcgtgatggc acacctatta tcttattaaa tgatcattac acgttaggta gctacccgca
     1561 aatcggtaca atcgcaagtt atcatttaac gaaattagca caaaaaccgc aaggatcacg
     1621 tttgaaattt caatttatag atattttaac ggctgaaaag aacctcgtta agtatagtaa
     1681 ctggttaaac caattattcc atggaataga atatagaatg caattagaaa tgatgaaata
     1741 atatttggta cgtagctcat actagagtcc gatgcaaata atttcctcct aatgtataat
     1801 gaaataatac tgtgttttat ctgcgaaatg tattatattc taattcgttt cacagtaaaa
     1861 tgaaaagata aagtgtgttt ttacttgaat tttgactaaa attactctat atttattaat
     1921 t
//
LOCUS       DATTQF010000226         1900 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.231, whole genome shotgun sequence.
ACCESSION   DATTQF010000226 DATTQF010000000
VERSION     DATTQF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.231"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1795
                     /locus_tag="VB241_002661"
     CDS             <1..1795
                     /locus_tag="VB241_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP1245876.1"
                     /translation="IDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTP
                     SFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNS
                     NVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAP
                     DSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYS
                     GRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAG
                     LKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVI
                     QLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELS
                     HDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPI
                     GMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYA
                     ENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINENGQETIESL
                     NHKLREATRIGDVELQKYYLQQIVAKNKERM"
BASE COUNT          720 a          250 c          350 g          580 t
ORIGIN      
        1 aatagatcaa tcgatcatta atgaaataaa agataaaacc gatattttag acttggtaag
       61 tgaatatgta aaattagaaa agagaggacg caattatata ggtttgtgtc cttttcatga
      121 tgaaaagaca ccttcattta cagtttctga agataaacaa atttgccatt gttttggttg
      181 taaaaaaggt ggcaatgttt ttcaatttac tcaagaaatt aaagacatat catttgttga
      241 agcggttaaa gaattaggtg atagagttaa tgttgctgta gatattgagg caacacaatc
      301 taactcaaat gttcaaattg cttctgatga tttacaaatg attgaaatgc atgagttaat
      361 acaagaattt tactattacg ctttaacaaa gacagtcgaa ggcgaacaag cattaacgta
      421 cttacaagaa cgtggtttta cagatgcgct tattaaagag cgaggcattg gctttgcacc
      481 cgatagctca catttttgtc atgattttct tcaaaaaaag ggttacgata ttgaattagc
      541 atatgaagcc ggattattat cacgtaacga agaaaacttc agttattacg atagatttcg
      601 aaatcgtatt atgtttcctt tgaaaaatgc gcaaggaaga attgttggat attcaggtcg
      661 aacatatacc ggtcaagaac caaaatacct aaatagtcct gaaacaccta tctttcaaaa
      721 aagaaagttg ttatataact tagataaagc acgtaaatca attagaaaat tagatgaaat
      781 tgtattacta gaaggtttta tggatgttat aaaatctgat actgctggct tgaaaaacgt
      841 tgttgcaaca atgggtacac agttgtcaga tgaacatatt acctttatac gaaagttaac
      901 atcaaatata acattaatgt ttgatgggga ttttgcgggt agtgaagcaa cacttaaaac
      961 aggtcaacat ttgttacagc aagggctaaa tgtatttgtt atacaattgc catctggcat
     1021 ggatcctgat gaatacattg gtaagtatgg caacgacgca tttactgctt ttgtaaaaaa
     1081 tgacaaaaag tcatttgcac attataaagt gagtatatta aaagatgaaa ttgcacataa
     1141 tgacctttca tatgaacgtt atttgaaaga actgagtcat gacatttcac ttatgaagtc
     1201 atcgattttg caacaaaagg ctttaaatga tgttgcacca tttttcaatg ttagtcctga
     1261 gcaattagca aacgaaatac aattcaatca agcaccagcc aattattatc cagaagatga
     1321 gtatggcggt tacattgaac ctgagccaat tggtatggca caatttgaca atttgagccg
     1381 tcaagaaaaa gcggagcgag catttttaaa acatttaatg agagataaag atacattttt
     1441 aaattattat gaaagtgttg ataaggataa cttcacaaat cagcatttta aatatgtatt
     1501 cgaagtctta catgattttt atgcggaaaa tgatcaatat aatatcagtg atgctgtgca
     1561 gtatgttaat tcaaatgagt tgagagaaac actaattagc ttagaacaat atagtttgaa
     1621 tgatgaacca tatgaaaatg aaattgatga ttatgtcaat gtaattaatg aaaacggaca
     1681 agaaacaatt gagtcattga accataaatt aagggaagct acaaggattg gcgatgtaga
     1741 attacagaaa tactatttac agcaaattgt tgctaagaat aaagaacgca tgtagcatgt
     1801 ggttttaaag aataatacga ataatgatta tgtcaaaatg tataagggta aatgatagtt
     1861 accgcattta aacaacacta ttgaaaaata aatattggga
//
LOCUS       DATTQF010000227         1879 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.232, whole genome shotgun sequence.
ACCESSION   DATTQF010000227 DATTQF010000000
VERSION     DATTQF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.232"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..110
                     /locus_tag="VB241_002662"
     CDS             <1..110
                     /locus_tag="VB241_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP1245877.1"
                     /translation="VAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            381..488
                     /locus_tag="VB241_002663"
     CDS             381..488
                     /locus_tag="VB241_002663"
                     /inference="COORDINATES: protein motif:HMM:NF028245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP1245878.1"
                     /translation="MISIANALHLMLSFGMFIVTFIGVVVAIINLNNKK"
     gene            1027..1128
                     /locus_tag="VB241_002664"
     CDS             1027..1128
                     /locus_tag="VB241_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245879.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            1138..1410
                     /locus_tag="VB241_002665"
     CDS             1138..1410
                     /locus_tag="VB241_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEP1245880.1"
                     /translation="MFGTILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HAHKGHGATAIGKYRSFSGYTRASVKAKASATKHNLRVNRAYYNIY"
     gene            1454..>1879
                     /locus_tag="VB241_002666"
     CDS             1454..>1879
                     /locus_tag="VB241_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEP1245881.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKY
                     NIHYLSDDELLKKDIKGSYFVKDKNFDVTKFINFLKEYGV"
BASE COUNT          687 a          245 c          300 g          647 t
ORIGIN      
        1 ttgttgcaca agtgatatta tcattaagtt atgagtatac tcgacgtata gcaacaacat
       61 ccgttgcaca aattgtagct attatattgt tattaatttt taatgcttaa ataagctttt
      121 cttagacgtc tgtgttctaa gaaaagcttt tttgctttag aatagcaata cgaagattcg
      181 aggcatgctt cattatagtg tacacgtttt taacggcaag ctgttattat tgagattagg
      241 ttttagtcag tttttattac taaatatttt ataaaaacag tataatattt tgcgagcaag
      301 tttaattgat ggtggctatc tgagtaaagg ggggtggtgc ctatggcatt acttaacact
      361 tttgaaagga aaagcctatt gtgatatcta ttgcaaacgc attacattta atgttaagtt
      421 tcggtatgtt tatcgtcact ttcattggtg tagtagtcgc aataattaat ttaaacaata
      481 aaaaataacc atcatttcaa aactttgaca acgaatgatg gttaaagaaa atctcattta
      541 gtcaccgtct ttttaacggg ctcattaggc gacatgttcc tgcatgtcgt ctttttacat
      601 ttttatagta acatatcttt tatttaattg tctattttta ttagatgtgt aacaaaattt
      661 tgaagtattt tccgtatatt gcctgctaaa catgtcagat ttgaaaggaa aatcatactg
      721 tggtagaaat caattatgat tatatggtag tacttataaa taagtgattt ctattgaata
      781 tgtaatgaag ttcatatatt aatataactt tcattgatat agaagtcgca atgattaatt
      841 taaacaataa aaaacaacca tcatttccaa actttgacaa agaatgatgg ttaaagcaaa
      901 tctcatttag tcaccgtctt tttaacgggt tcaatagggg acatgttcct gcatgtcgtc
      961 tatttatgca ctgcgcaatt taataataat acaattatac tttgatgtcc atttaatata
     1021 aagtggttga aatttgcagc aaataaatta accttgacta ttttcagaaa attctgtata
     1081 aataacgaaa aagagatatg gattaaatat gaagaggtgt ttttatagaa gaataaaatt
     1141 tttggcacta tattatattt atctttagca cttggattat caacagcagc ttatgcatct
     1201 acagaatacg ctgaaggagg cacttggagt cacggtgtcg gcagtaagta tgtttggtcg
     1261 tattattatc atgctcataa aggtcatggt gcaacagcta tcggaaaata tagatcattt
     1321 agtggttata caagagccag tgtaaaagca aaagcatcag ctactaaaca taatttgcgg
     1381 gtcaatagag cgtattataa catttattaa atactaaaaa atcatagaaa gtaaacaacg
     1441 caagaggtac aacatgaaat ggttcaaatt aatacttgat gttacaatct tcattctgat
     1501 tgctatattg ttatttgttt atacatataa agaaaacgaa gaaatattgc ctgatactaa
     1561 ataccctata gcggtaactg actggaataa aaaatatagt aaaaatgaga tttataaacg
     1621 tataaatcaa ttcgctaaaa atgagaacgt agcaatctat aaatcaactt caaattatac
     1681 aaacaaaaac gtagataaag atatatatgt atttaataaa tcaaaagcaa caactatcac
     1741 tccttttaat gctaaatata acattcatta tttaagtgac gacgaattat taaaaaaaga
     1801 tatcaaaggg agttattttg taaaagacaa aaattttgac gtgactaaat tcataaattt
     1861 tttaaaagaa tatggtgtt
//
LOCUS       DATTQF010000228         1871 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.233, whole genome shotgun sequence.
ACCESSION   DATTQF010000228 DATTQF010000000
VERSION     DATTQF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.233"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(176..1822)
                     /locus_tag="VB241_002667"
     CDS             complement(176..1822)
                     /locus_tag="VB241_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEP1245882.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKYKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSRMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          683 a          360 c          278 g          550 t
ORIGIN      
        1 taaataaacc tattttaaaa tataaaaaaa tcgagaattc gtagtttaat aacgaaattc
       61 tcgttcttat cattttgaat atactcaatt ttccacaaaa ataaacaagt agtatatctg
      121 ttctagctac tagaatgaca tactacttgt tattaaaata cttgactaaa ttttattagt
      181 tatctttttt ctctatattt ctacgtgact gacgcttttc aagaatgtca gattcataat
      241 cttcttgttg actcttgata tattcttgta agcggtgttt attcggagtc aatgttaaac
      301 ctaggaattt acgttcctgg tttgcatcct tgtagaaact taccatcatg agtatgacga
      361 caaaggagaa tgggaatgca cttataattg cagcactttg aatcgcattt aaagcttcag
      421 caccgttacc gccaccagct aataaaagta caaatgctat taaggcctgt gaaattcccc
      481 aaacaacttt taccattcta gatggattta atgaaccaaa tgttgtttgc attcctaata
      541 caaatgttgc tgagtcagca gatgtaataa agaatgatgc aattaataat aatgcaatca
      601 acgataaaac aatgccaaat ggcacatgat taaacactcc aaatagctgt gtttcaggag
      661 tcatatcaaa aatttctttg tatttcttac ctgtctcgat gcctaataca ccaaagacac
      721 taaaccatac aaaactaaca attgctggaa ctagcaagac accagaaatg aactctctaa
      781 ttgaacgtcc ttttgaaact cgtgcaataa acacaccaac gaatgggctc caacttaacc
      841 accaacccca gtaataaagt gtccatgaag acatccattc acgtttttga ggatttaaag
      901 cagctgtatc aaaactatta aacaagaatg tgtttagtaa actacccgta gagctagtta
      961 acatatttaa aataagaaca gttggtccaa caattaaagc agctaccatt aaaatagtac
     1021 ctaaaccaat gttcaagtta cttaagtatt gaataccttt acttaatcca gaccatgcac
     1081 ttgctataaa taagatagta acaacaatga tgataatcgc ttgtacaaac gtattgtttg
     1141 gaacattgaa caagtaatgt aaaccaccat taatttgtag agcacccata cctaacgaaa
     1201 cagctacccc aacgattgtc gcaaatacag ataaaacgtc aataaaaatc ccaataggac
     1261 cttctacttt atcacctaaa agaggacgta aagttctaga taataaacct ggttcacctt
     1321 tacggaattg cgaatatgcc aacgctaacg caacaacacc ataaacagcc caagcatgga
     1381 atccccaatg gaaaaatgtt gaacgtaggg cttcagtata agcttcagta gttttgggat
     1441 ctgccgtagg tggcgtagca aagtgcgcca tcggttcagc tgcaccataa aacaccaaac
     1501 ctatccccat accagcacta aacaacatag caaaccatga aattgtatta aactcaggtt
     1561 tgtcatttgg tttacctagt ttaagttttc caataggact aaaaataagg aatatacaga
     1621 agaacacgat aatcgtagta agaataagat aataccaacc taacttttct gtaatccaca
     1681 ttttaatatt attggtaaca tagttgaatt gttcaggtaa aaatgcacca agtaatacga
     1741 ctatagcaac aacaattgca ctatagatga agactggtga atacttcttt ccatttggat
     1801 tctctggtga agaagaattc ataattaatt actcccttca attctatatt taattttatg
     1861 tagtagaata a
//
LOCUS       DATTQF010000229         1838 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.234, whole genome shotgun sequence.
ACCESSION   DATTQF010000229 DATTQF010000000
VERSION     DATTQF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.234"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            59..598
                     /locus_tag="VB241_002668"
     CDS             59..598
                     /locus_tag="VB241_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP1245883.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEVYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            611..1705
                     /locus_tag="VB241_002669"
     CDS             611..1705
                     /locus_tag="VB241_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245884.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            1698..>1838
                     /locus_tag="VB241_002670"
     CDS             1698..>1838
                     /locus_tag="VB241_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245885.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQI"
BASE COUNT          688 a          221 c          336 g          593 t
ORIGIN      
        1 ttaaacaaaa tgtttgataa aagtaaactt aatatagctt ttttaggtgg aaagataaat
       61 gaacataggt aataaaatta aaaatcttag aagaattaaa aatttaacgc aagaagaact
      121 tgctgaacgt actgacttat cgaaaggcta catttcacaa atagaaagtg aacatgcctc
      181 accaagtatg gaaactttct taaatattat agaggtgtta gggacgacgc caagtgaatt
      241 ttttaaagac agtgaaaatg aaaaagtatt atacaagaag gaagaacaag ttatttatga
      301 tgagtatgat gaaggttata tattaaattg gttagtttca aagtcaaatg aatatgatat
      361 ggagccatta atattaactt taaagcctgg cgcatcatat aaaaatttta atccatcaga
      421 gtctgatacg tttatttatt gtatgtcagg tcagataaca cttaatttag gcaaagaggt
      481 atatcaagca caagaagaag acgttttgta ttttaaagca cgagataatc atcgtttgtc
      541 aaatgaatca aacaatgaaa cacgaatact tattgtagcg acagcttcat atttataggg
      601 gggatcttat ttggaaccgt tattatcatt aaaatcagtt agtaaaagct atgatgatct
      661 taatatctta gatgacatag atattgatat tgaatcagga tacttttata cattattagg
      721 tccttcaggt tgtggtaaaa caacaatttt aaaattaatt gcagggtttg aatatcctga
      781 cagtggtgaa gtgatttatc aaaacaaacc aattggtaat ttaccaccaa ataaacgtaa
      841 agtgaataca gtctttcaag attatgcatt atttccacac ttaaacgtct atgataatat
      901 cgctttcggt ttgaaattaa aaaaattatc aaaaaccgaa attgatcaaa aagtaactga
      961 ggcattaaaa ttagttaagc tttcaggtta tgaaaaaagg aatattaatg aaatgagtgg
     1021 cggacaaaag caacgtgttg caattgcacg tgctattgta aatgaaccag aaatattatt
     1081 gttagatgaa tctttatccg cattagattt gaaattgcgt actgaaatgc aatatgaatt
     1141 acgagaattg caatctagat taggtattac atttatattt gtaacacatg atcaagaaga
     1201 agctttagca ctaagtgact ttctttttgt attaaaagat gggaaaattc aacaatttgg
     1261 tacaccaaca gatatatatg acgaaccagt gaatcgattt gtagctgatt ttattggaga
     1321 atctaatatt gttgaagggc gcatggttag agattatgtc gtgaatattt atgggcaaga
     1381 tttcgaatgt gtcgatatgg gtattcctga aaataaaaaa gtagaagtcg ttattcgacc
     1441 agaagatata tcattaatca aagctgaaga aggattattt aaagcaactg ttgattctat
     1501 gttgtttaga ggggtccact atgaaatatg ttgtatagac aataaaggtt atgaatgggt
     1561 aatacaaacg actaaaaaag ccgaagtagg cagcgaagtt ggtctttatt ttgatcctga
     1621 agccattcat attatggttc ctggtgaaac agaagaagag tttgataaac gtattgaaag
     1681 ctatgaggaa gtagacaatg cgtaatacta ataaatttct tttaatcccg tatttactat
     1741 ggatggttat atttattatt gtaccagttg tattactcat ttatttttca tttttagata
     1801 tcaatggaca ttttagtttc acgaattatc aacaaatt
//
LOCUS       DATTQF010000230         1805 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.235, whole genome shotgun sequence.
ACCESSION   DATTQF010000230 DATTQF010000000
VERSION     DATTQF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.235"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..693
                     /gene="seg"
                     /locus_tag="VB241_002671"
     CDS             <1..693
                     /gene="seg"
                     /locus_tag="VB241_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HEP1245886.1"
                     /translation="PKIDELNKVSDYKSNKGTMGNVMNLYMSPPVEGRGVINSRQFLS
                     HDLIFPIEYKSYNEVKTELENTELANNYKGKKVDIFGVPYFYTCIIPKSEPDINQNFG
                     GCCMYGGLTFNSSENERDKLITVQVTIDNRQSLGFTITTNKNMVTIQELDYKARHWLT
                     KEKKLYEFDGSAFESGYIKFTEKNNTSFWFDLFPKKELVPFVPYKFLNIYGDNKVVDS
                     KSIKMEVFLNTH"
     gene            complement(1268..1805)
                     /locus_tag="VB241_002672"
                     /pseudo
     CDS             complement(1268..1805)
                     /locus_tag="VB241_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
BASE COUNT          633 a          211 c          275 g          686 t
ORIGIN      
        1 cctaaaatag acgaactaaa taaagtaagt gattataaaa gtaataaggg aactatggga
       61 aatgttatga atctttatat gtctccacct gttgaaggaa gaggagttat caattctaga
      121 cagtttttat ctcatgattt aatttttcca attgagtata agagttataa tgaggttaaa
      181 actgaattag aaaatacaga attagctaac aattataaag gtaaaaaagt agacattttt
      241 ggtgttccat atttttatac atgtataata cctaaatctg aaccggatat aaaccaaaat
      301 tttggaggtt gttgtatgta tggtggtctt acatttaata gttctgaaaa tgaaagagat
      361 aaattaatta ctgtacaggt aacaatcgac aatagacaat cacttggatt tacaataact
      421 acaaataaga atatggttac tattcaagaa ctagattaca aagcaagaca ctggctcact
      481 aaagaaaaaa agctatacga gtttgatggt tctgcatttg aatctggata tataaaattt
      541 actgaaaaga acaatacaag tttttggttt gacttatttc ctaaaaaaga actagtacct
      601 tttgttccat ataagttttt aaatatttac ggagataata aagtagttga ttctaagagt
      661 attaaaatgg aagtatttct taatactcac tgataagtat atttgatatc actatacaat
      721 cataaagtgc gtttttcgaa cttttgttct aagtgtttta tagattcatg taaatagtta
      781 gtttattgct ggttgaatta tttaattttt ctcaatgatt tattctaaat atgttttatg
      841 tcaatagcgg tttaaaaatg ctgtttaatg gcggtttgaa aatgacgttt tttagaggtt
      901 taatattgtc gtgactttaa atgtattttt actttaataa tcttttagtc gccatgattc
      961 tccagtggaa atgataattt aataacgtat acttttgatt gtttaagaat gtacaatttt
     1021 ttaacactct agagataaat atatcgaggt aatccgcctt ctccgttaat ttaacggaaa
     1081 tacaatgaaa tgttagtatt cttaaaagag caagtgtcaa atacgtatca agaaaaagat
     1141 tttctgattc gttgaccttg ctctttttgt tctaaaataa aacgtgcaaa taaaaaagta
     1201 tctacttttt tacttgcacg atatgatttg aaaattaaag aattacaaat aggggtctaa
     1261 tgttaattta tgaggttaaa gtttcaatga tttttgattt attagaccat tttaatatct
     1321 caaggttctc atgttctgct acagtttgtg ctctttcatt tatcggatga tatacatcat
     1381 ttacaattat taacatgata ggttctaatt tgtttctgga aggtctgttt ggtttgatat
     1441 cacggtaaat ataagcttca ttagttattt tgttaaaatc taagttattt gtaaagttaa
     1501 ctagtttttc tggttgtgat tttgtttctg aaacaatata atcaatagaa tattttaaac
     1561 ctgattctcc agaaacagaa acttgtgcta aacctcttat ttctcggtca tataagaatt
     1621 caaatacttc ttcatagaat ctgtttgtaa cgttagattt agtagttaaa gtaaggtcat
     1681 aaattttcaa tatgccttga attaaattat gtttagactg agcaaaactc taatttttaa
     1741 catccgctgt aatttcttta tcaactaatt ttaaattaaa ttgatttaaa atattctgta
     1801 taagt
//
LOCUS       DATTQF010000231         1799 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.236, whole genome shotgun sequence.
ACCESSION   DATTQF010000231 DATTQF010000000
VERSION     DATTQF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.236"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            278..1303
                     /locus_tag="VB241_002673"
     CDS             278..1303
                     /locus_tag="VB241_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEP1245887.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          624 a          215 c          326 g          634 t
ORIGIN      
        1 gttccattta aatcccctct tttctctatt tcctaaatca tagaactttt aacagtaaaa
       61 ttcaatcatt aatatatgtg tttcggcacg caaagtaatt gtggtaaaaa aattaattaa
      121 ttttcataat gtattgtttg atgtataata tatttattca acttcgcgat aatgcgatac
      181 aaactttaac cattcataat acattttaaa tatcgaacta gttttgaaat tggtttatct
      241 tattaaaatg ttgatattag ttttaggggg aaatgtagtg atttcattcg aaaatgatta
      301 tttagaaggt gctcatgaaa aagtattaaa acgattagta gatacaaatc ttgtacaagc
      361 ttcgggatat ggctttgatc aattcacagc tcaggcaatt gaaaagatta aagatacaat
      421 tgactgtcca aatgctacaa ttcgtttttt agtaggtgga acacaaacca atcaggttgt
      481 tattaattca atgttagaaa gttacgaagg ggtcatttca gcagacactg gtcatgttgc
      541 tgtgcatgag ggcggtgcaa ttgaatatag tggacataaa gtaattacta taccctctaa
      601 ggaaggaaaa gttagtgctt cagatgttga aacttacatg gagacattta aaagtgattt
      661 taaaaaggac catatggtat tcccaggaat ggtatatatt tctcatccta ctgaatatgg
      721 cacattatac tctaaatcag aattggaaga gttatgcaaa gtatgtaagc aatatcagct
      781 tccattattt atggatggtg cacgattagg gtatggattg atgagtgatc aatcagatat
      841 gactattaaa gacatcgcta aatattgtga tgtattttat ataggtggta cgaagatagg
      901 tgcattatgc ggagaagcaa ttgtttttac gaagaataat gaaccgaaac aatttaccac
      961 tcgaatcaaa catcacggtg cacttttagc aaaaggacgg ttaacaggca tacaattttt
     1021 agaattattt acggataatt tatattttaa tataagcaga catgctatcg aaatggcaaa
     1081 taagatgaaa gacgggttta taaataaagg ctatcgtctt tactttgatt ctccaaccaa
     1141 tcaacaattt tttattttaa gtaacgagaa aatagcagag ttagaacaaa aagttaaatt
     1201 tgctgtttgg gaaaaatacg atgatcaaca tcgtgttgtt agattcgcaa ccagttgggc
     1261 gacaaccgaa gaaaatttaa ataaactatt agaattaatc taataatgct gttatatgtc
     1321 atctggcaaa tcatttatta attaatcaag atcagttgag tagttgtgtt tatttatata
     1381 tgaaactgct catttgtagc cgttgatgat ataaaattac attattccaa aacaaggtac
     1441 tacagattat tgagtatctt gttttatttt atatatttaa aattgttaat ttgtgctgta
     1501 tttactttaa tttgacgaga tttgagaagt attatgatgg ttgtagttat tttatgaaag
     1561 taatgtatta acaatcgaat tactaagtct atattggtag aaatatcgat gtttagttta
     1621 aatggaatgc actatttaag tttttaatat ggagatgttt gtgacttttc gatgattaag
     1681 atttttatag gtgtgcatct ttacaaataa actttgtgtt taaaattgag cttaggaaat
     1741 cgataggttt agatgaggat attgttgaag gtatgtgtct tgtatcctta gttgttaaa
//
LOCUS       DATTQF010000232         1797 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.237, whole genome shotgun sequence.
ACCESSION   DATTQF010000232 DATTQF010000000
VERSION     DATTQF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.237"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(117..512)
                     /gene="spxA"
                     /locus_tag="VB241_002674"
     CDS             complement(117..512)
                     /gene="spxA"
                     /locus_tag="VB241_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP1245888.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            807..1796
                     /gene="trpS"
                     /locus_tag="VB241_002675"
     CDS             807..1796
                     /gene="trpS"
                     /locus_tag="VB241_002675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP1245889.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKASFKTVKKMEKAMGLGRKR"
BASE COUNT          657 a          268 c          292 g          580 t
ORIGIN      
        1 ttgatttaaa agatactaag caattattgt aaatttaaaa ccaaaaaagc aacactatag
       61 aaatagcgtt gcttgacata tcttttagta aagtatgaca catagttaaa tggttattag
      121 tcaaccatac gttgtgcttc ttgtaattgg aacgtacgaa cttttctagg taagaaacgt
      181 cgaatctcgt cctcattata accaacttgt agtcgtttat tatctaaaat aattggacga
      241 cgtaataagc caggattatc ttgaatgatt gaatataagt cttgtaatgg tagtgaatca
      301 atatcaacat ttaatttttg gtatgtttta gaacgtgtag aaatgatttc atcagtaccg
      361 tcttcagtca tttttaatat ttgcttaatt tcatcaattg ttaaatgttc agaaaaaata
      421 ttacgctccg tatacggaat gtcatgttct tgtaaccatg ctttcgcttt acggcaagat
      481 gtgcaacttg gtgaagtaaa taatgttacc atacatctca ctctcctatt tgaatgaata
      541 aaattcattg cttaaaattt agttatagat caagaaaaaa catttttttc taaaattctt
      601 aatcgttact atttattata actatctaac attaaaatta aatgagaaaa acctaatttt
      661 tcagataagt ttctacactt ataaaaaaga ttattaatcc ctttgttagt agcaagttat
      721 acatatattc taacacatct tacattttta agaaatactg ttataatgat aattgttaaa
      781 ttattactaa gaaagtaggc atttaaatgg aaacattatt ttcaggcatt caacctagtg
      841 gaattcctac tattggaaat tatattggcg cattaaagca atttgttgat gtgcaaaacg
      901 actatgattg ttatttctgt atcgtagatc aacatgcaat tacaatgcca caagatcgct
      961 taaaattacg taaacaaact agacaattag cagcgattta tttagcttct ggtatagatc
     1021 cagacaaagc aacattgttc atacaatctg aagtccctgc acacgtacaa gcaggatgga
     1081 tgttaactac aattgcttct gttggcgaat tagagcgtat gacgcaatac aaagataaag
     1141 ctcagaaagc agttgaaggt ataccagctg gtctattaac atatccacct ttaatggcag
     1201 ctgatattgt tctttacaat actaatatcg ttccagttgg cgatgaccaa aagcagcaca
     1261 ttgaattaac acgtaatctt gtagatagat ttaatagtcg ttataatgat gtccttgtga
     1321 aacctgaaat tcgtatgcct aaagtcggtg gccgtgttat gagtttacaa gatccaacaa
     1381 gaaaaatgag taaaagtgat gacaacgcta aaaactttat ttcattatta gacgaaccaa
     1441 atgttgctgc taaaaaaatt aaaagtgctg tgactgattc agacggtatt attaaattcg
     1501 accgcgacaa taaaccaggt attacaaatt taatttcaat atacgctgga ttaacagaca
     1561 tgcctattaa agatattgag gcaaaatatg aaggcgaagg ttacggtaaa ttcaaaggcg
     1621 accttgctga aatagttaaa gcatttttag tagaattcca agaaaaatac gaaagtttct
     1681 ataactcaga taaacttgat gatattttag atcaaggtag agataaagca cataaagctt
     1741 catttaaaac tgtcaaaaaa atggaaaaag cgatgggttt aggacgtaag agataaa
//
LOCUS       DATTQF010000233         1726 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.238, whole genome shotgun sequence.
ACCESSION   DATTQF010000233 DATTQF010000000
VERSION     DATTQF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.238"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..664
                     /locus_tag="VB241_002676"
     CDS             <1..664
                     /locus_tag="VB241_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEP1245890.1"
                     /translation="EPNELNTRYDKEIDHLIDYFILPLRQIEQVSIARSKIICDVDLI
                     NENNDLKEKITMSDINKVTELLTNYTKFRGLIMHMSIISEASKEINLTRLSIFIFIIN
                     ILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSCKGLQQFSHAKT
                     KQIVLSIDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVSSNSIVHLKYHL
                     EI"
     gene            690..1433
                     /locus_tag="VB241_002677"
     CDS             690..1433
                     /locus_tag="VB241_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HEP1245891.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKARDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLLNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            1497..>1726
                     /locus_tag="VB241_002678"
     CDS             1497..>1726
                     /locus_tag="VB241_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245892.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGANEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTI"
BASE COUNT          655 a          191 c          297 g          583 t
ORIGIN      
        1 ggaaccaaat gagttgaata cgcgctatga taaagaaatt gatcatctaa tagactattt
       61 tatcttaccg ctaagacaaa tagaacaggt gtctattgca cgttctaaaa ttatatgtga
      121 tgtagatttg attaatgaga ataatgactt aaaagaaaaa atcacaatgt ctgatattaa
      181 taaggttact gaacttttga caaattatac aaaatttcga ggacttatta tgcacatgtc
      241 aattataagt gaagcgagca aagaaataaa tttgactaga ttatcaatat tcatttttat
      301 cataaatata cttaatcaat tacgagggat tgttatttat caaaatgaca cgatgattgt
      361 aacgaagttc aaacatgaag tacaatgtgt catttgtttg ccagccgcat tgttaaatga
      421 gaattcagac gctatacgtt tatcgtgtaa gggtcttcaa caatttagcc atgctaaaac
      481 taaacaaatt gttttaagta tagataaaga taatcatact tccgaaaatg gtgtacttgt
      541 gaatgatatt atgcaacaac acagttattg gaaagctaat tttatagaaa gaaatattta
      601 tgatcattca ttaatggtct caagcaatag tattgttcat ttgaaatatc atcttgagat
      661 ataggcatgg tttaagagag gttttaaaga tgtctaaatt taaagttaag cgtttaactg
      721 cacacatact aatgttgaaa gtcattaatg atatgttgaa atattcattt catctcactt
      781 tgactcaagt gaaactatta aatctattag ttaaatatga taattctgat tccaatagcg
      841 cttcaataga taggttatta aaaatgaaag aaatccaatc taagatggca cttcttcaaa
      901 tgttaagtta tttaaacaat catcaatggc tattgaaagc aagagatcaa agggatcaac
      961 gtaaattgac tatttcgatt aataaaatgc atatagataa aatagaatat atgaataatg
     1021 agcttagtga ctatattgaa cgttattttg gtcaatatac atttgatttt agttgtgact
     1081 atgtatcgta tttattgtcg tcacaagaat tattgctaaa tctaaagtgt tatttaaata
     1141 tgtgtcaatt atcattagaa gagttatacg ttttaggaat attaaatctg cataaaggtc
     1201 aattgacggt taaagaatta cagggtgaat tccatcaccc gatatttgcg gttagtccga
     1261 ttttaaagtg tttaattttg aaaggattag ttaaaaaaga gagatgtgaa ttagatgaaa
     1321 ggcgtgttat tgtaacaatc aagcgggaaa agttttctaa agtcactatg cttattcaag
     1381 catgttataa ttatcttgaa aagggaatac aagttaagct taataataaa tagttaatca
     1441 cttgtatttc atatacctat gaagaatagt ttatagtttt aagtgggagg gatattatga
     1501 ttttagataa tgtgaatcca aatgatttat ttccaactga aaaaaagggt ccgtcagtgt
     1561 taggaataat tgagtatcaa gttcaaggtg cgaatgaatt tgaaggggca tttatagcaa
     1621 caaatgaaag attaattatg aatgtagata tgaatggcca attttattat agaagtatta
     1681 gttataatga aatagaaaag atagattatg atggtcaaac tataat
//
LOCUS       DATTQF010000234         1656 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.239, whole genome shotgun sequence.
ACCESSION   DATTQF010000234 DATTQF010000000
VERSION     DATTQF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.239"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            147..467
                     /locus_tag="VB241_002679"
     CDS             147..467
                     /locus_tag="VB241_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEP1245893.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKDDTPKE
                     VRQE"
     gene            464..1105
                     /locus_tag="VB241_002680"
                     /pseudo
     CDS             464..1105
                     /locus_tag="VB241_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1193..1483)
                     /locus_tag="VB241_002681"
     CDS             complement(1193..1483)
                     /locus_tag="VB241_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245894.1"
                     /translation="MFFACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYVA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILFFFSLPLLLS"
BASE COUNT          671 a          202 c          259 g          524 t
ORIGIN      
        1 attggaatat tgacgaatgt aattttacat tctcactata taatgatgat atttgcaacg
       61 attattgtcg ttcaaatatt attgcagatt tgcagcctat actatcatgg ccgacgtttt
      121 aatgaagtta tcaaggagtt ttaactatga aatatatcat tgcaatatta ttaggattaa
      181 tcgtatctat tactattgct tttacaatca tacatcatcc tatacttgat cgttttaatc
      241 ctttcttaaa aacggaatat agttatgcca aagtgccaaa aggtacgcaa caatatgtta
      301 atattacggc ttatagtgaa agaggggaaa agcttgatta taaattaaca tttaatggat
      361 tttctcctag tagaacgtat gtcgaaataa agcataaagg gcaatatgtt atatcaatca
      421 catatgttga aaaagatgat acaccaaaag aggtaagaca agaatgatag aattaaaaga
      481 tttaaccata caaaaaggta atacacatat tttgaagaaa cttaatttga aatttcaatg
      541 tggaaaatca tatgcactta ttggtaaaag tggatgcggt aaatctacat tattaaatac
      601 tattgctgga cttgaaaaaa caggaaaaca atatgtctat tttaatggtc aattagaaca
      661 attttaatct aatttttaca gagataaatt agggtattta tttcaaaatt atggattaat
      721 cgttaatctg acagtaaatg aaaatttaga tattggatta gcatataaaa aaataagtaa
      781 gaaagaaaaa gaacaattaa agatacgtta tatagaacag tttggtctgt caaacagttt
      841 aaaaagaaaa gttcatacgc taagtggagg tgaacaacaa cgtgtcgctt taattagaat
      901 gatgttaaaa gatccgattg ttatgttagc tgatgaacca acgggtgcgt tagatcctaa
      961 aacaggacag atgattattc aatcattatt tgatttgatc gatgaaaata aagtgctgat
     1021 tttagcaaca catgatatgg ctattgcaaa tcaatgtgac gaaataatag atttagaaca
     1081 gtatagaaaa gtagcatcta tgtgatatcc aaaagataaa attaaaagcc aatcaattgg
     1141 gaatgttaat ttcaacactc catttgattg gcttttataa tgcgttgcta tgttatgaaa
     1201 gtaacaacgg caaactgaaa aaaaacaata taataatatt taatacgagt agtaataaat
     1261 gtaattgatt ttttgtttta atgtagcaaa aaacaatgac taaagatagc agcaatatca
     1321 tgattgcacc tgggaagaac cataatgctg aagctacata ccaaatcatt ggtaacgcca
     1381 taataataaa agatataaga caagaaagga taccaataat attataagtt ctagtcaaaa
     1441 ttacacctcc aattttcatt ttaaaatgac atgcaaaaaa caatcatttt aaatacatca
     1501 tacaataaaa ataaattata tgatagatga atttgaaatt aaattgaatt ggcttatcat
     1561 tcgtaaacat tattatgacg taacaattct tataagtgga acgagtgtaa ttataccatc
     1621 tgtatagaga gtcgtatcaa tcgtaaaatt taatct
//
LOCUS       DATTQF010000235         1605 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.240, whole genome shotgun sequence.
ACCESSION   DATTQF010000235 DATTQF010000000
VERSION     DATTQF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.240"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1226
                     /locus_tag="VB241_002682"
     CDS             <1..1226
                     /locus_tag="VB241_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEP1245895.1"
                     /translation="KIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATESDLQG
                     STGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDELIYIT
                     GSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGISERTKVIAIQR
                     SKGYDQRPSIPLNEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADLIAGSL
                     IKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFFLAPHV
                     VSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQHASPI
                     NAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTYEHVKI
                     AVTRAVMQLKEQGLI"
     gene            1470..>1605
                     /locus_tag="VB241_002683"
     CDS             1470..>1605
                     /locus_tag="VB241_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP1245896.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     E"
BASE COUNT          570 a          221 c          323 g          491 t
ORIGIN      
        1 gtaagatagt agctgacgtc gaatcaacgt tagcaccata ttttaaagaa attgaagaaa
       61 ctgcatatat taaccaagaa aaagtattaa atgcatttca tcatgtcaaa gcaaccgaaa
      121 gtgatctaca aggatcaaca ggatacgggt atgatgattt tggacgtgat catttagaag
      181 aaatatatgc gcaggcattt aaagcagaag atgcaattgt tcgcccacaa attatttcag
      241 gtacgcatgc gattactatt gcattacaaa gtttattaaa acatggtgat gaattaattt
      301 atataacggg aagtccatat gacactttac ttgaagtcat tggtgtaaac ggaaatggta
      361 ttgaaagttt aatggagcac ggcgtatcat ataaagatat tgcacttaaa gaaggtaaga
      421 tcgatattga aagtgtgtta gatgggattt ctgagcgcac caaagtaata gcgattcaac
      481 gttcgaaagg ctatgatcaa agaccttcaa ttcctctaaa tgaaattgaa caggtgatta
      541 ctaggttgaa agaagtgcat cctaatattt taatatttgt ggataactgt tatggggaat
      601 ttgttgaaag acgtgaacct atagaatgtg gtgccgattt aatagcagga tcattaatta
      661 aaaaccctgg cggtggttta gctaagattg gtggatacat tgctggtaga aaagatttaa
      721 ttgaacgatg tggttataga ttgacagcac ctggtattgg taaagaagcg ggtgcatcat
      781 taaatgcatt gcttgaaatg tatcaaggtt tctttttagc accacacgtt gtcagtcaga
      841 gtcttaaagg tgcattgttt actagtttat ttttagaaaa aatgaatatg aacacaacgc
      901 cgaagtacta cgaaaaacga actgatttaa ttcaaacagt taaatttaaa acgaaagaac
      961 aaatgatttc attttgtcaa agtattcaac acgcatcccc aattaatgca cattttagtc
     1021 cagaacctag ttatatgcct ggttacgaag atgatgttat tatggcagct ggtacgttta
     1081 ttcaaggttc atcgattgaa ttatctgcag atggacctat tcgtcctcct tatgaagcat
     1141 atgttcaagg aggattaaca tatgaacacg ttaaaattgc tgtgacaaga gctgttatgc
     1201 agttgaaaga acaaggactt atataataga ttaaatttca ccctaaatga taatatgttt
     1261 tgggtgaaat ttttttgcga atatttatca gattattttt aaaaattgaa aaaatatttt
     1321 agctgtaggt cgttttatgg cgttttagag gtcgaaatcg cagttatatc aatgttttga
     1381 gctttatgtt agaaaacctg acatattttt gaaatcctaa aaaaattatg ataagttatt
     1441 aacaagttca aaagtagagg agaggaacaa tgatatcgaa tgatgcaatc agacgaaata
     1501 tggctgtctt ctctatgagt gtagtaagta agttaacgga tttaacgcca aggcaaatac
     1561 gttactatga aacacatgaa ctcatcaaac ctgaaagaac agaag
//
LOCUS       DATTQF010000236         1596 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.241, whole genome shotgun sequence.
ACCESSION   DATTQF010000236 DATTQF010000000
VERSION     DATTQF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.241"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            139..1092
                     /locus_tag="VB241_002684"
     CDS             139..1092
                     /locus_tag="VB241_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245897.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKFEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVMPALAYFTFSPKNYEDKSNPDPKVLN
                     LVGMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1255..1458)
                     /locus_tag="VB241_002685"
     CDS             complement(1255..1458)
                     /locus_tag="VB241_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEP1245898.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
BASE COUNT          621 a          214 c          256 g          505 t
ORIGIN      
        1 acaaaacaat tttcttaaat tatcaattaa agtatttaaa aatattgatg gttaatgtaa
       61 aatatcaata taaataagtt ttttaaattt atgtatgttt atttgattca aacaaaataa
      121 cttaagagga gaaagtttat gaaaaagaca ctgggatgtt tacttttaat tatgctttta
      181 gtcgtagcag gttgttcttt tggtgggaat cataaattat catcaaagaa atcagaagaa
      241 tcaaaacaag aaactgtaaa aaaagaatcg gaagaagaga aagatccaga tttagagaaa
      301 tttgaagaaa tagagaagaa aatgaaagga attaaagatg cgccatctct agataagttg
      361 gatccattaa tgacagaaaa gtcgtttacg aatagtaaag ggattcaagg atggaaagat
      421 tacaaagaat taatgggtaa agtggaactt gcagattata gatttacaaa agattcaaaa
      481 ggatcttcta taaaagatgt tgatgcattc tttaaaggta agaaaggtat aaaaaggaaa
      541 gtgattgaaa cacacgatga tgtaaaacaa gttgattatt ggtatgtaga tccagatgga
      601 aagaaaattg gcaattcaaa cacacctgtt ttttacgcag aaattatgac aaaatataaa
      661 gatggaaagt tagtttatgc atcagtcgaa ccaggttctt acgtaataca taaagatgat
      721 gcaattaaat atgacgatta ttctaagtta aaaaaattaa gtcaactaac taaacttgat
      781 catccaaagc cagttccata tagcgtagct caaatcaaat ctttcggagt acctttaaca
      841 agcgtttcat ttatgacgca tggatcaaag gatactaaag atgaagtgat gccggcattg
      901 gcatatttca ctttttcacc aaaaaattat gaagacaagt ctaatccaga tccaaaagtt
      961 ttaaatttag tagggatgga tttcttaaat gcatctagtg attttggtaa cgcacatttt
     1021 gttgttctaa gtaaatatat taaagagtac gaatcaaact atgaaacagc gtcagatgat
     1081 tctttaaaat agtatttact gtgcggaaaa taaatagtgt gctacattaa ataatcgcaa
     1141 taataatccc gataaacaat cagcattgct gcttatcata tagagttcgt aataactata
     1201 actctatgat tcgcaaataa taaatgattg tcatcgggat ttatttttat caatttataa
     1261 agtgacatta ccttgttcat cagcaggttt gaaaacagta atcactgcac taataattgc
     1321 taaaatgtgt gggatacctg tccaacagaa tattaagtgt agaatacctt gcatattctt
     1381 gccggcataa aatttatgaa taccaaaact acctaagaac aatgctaata aaatataaat
     1441 aactttgttt acttgcattt ctttccctcc agttgaattg cttataatga cattagcttc
     1501 tctttttatt atacccactt ttagttcaaa cattctagtt tcagcattcc caatcatcta
     1561 aatttcattt tttcaatcct tacaataaat ttagga
//
LOCUS       DATTQF010000237         1590 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.242, whole genome shotgun sequence.
ACCESSION   DATTQF010000237 DATTQF010000000
VERSION     DATTQF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.242"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            1..575
                     /locus_tag="VB241_002686"
                     /pseudo
     CDS             1..575
                     /locus_tag="VB241_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            967..1503
                     /locus_tag="VB241_002687"
     CDS             967..1503
                     /locus_tag="VB241_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245899.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
BASE COUNT          666 a          247 c          230 g          447 t
ORIGIN      
        1 aattgctata catagcgatg ttacaagcaa taaaaaaaga tattataaat catatagtag
       61 aggaacagtg gtatatgtaa aactagggag taatattgga agtgagtttt ctggtaatca
      121 tttttgtgta atattagata acaaagacaa taaaggaaaa gaaacagtta caatagttcc
      181 actttcttcc aaaggtaata aaaattattt gaagttaaac gaatcggtgc taaatctaac
      241 aacaacagat ttaaaaaaaa cagattatcg atatcagtag taaagtacaa gcattagccc
      301 aaaaatatag agatattgac attaaactta gcacagaaga taaaaagctt ttgtctaact
      361 taaatcaaaa agctaatgaa ctgtatagag tgataggtgt ttattccaag cacaaaggga
      421 aagatacata cgttaatata tcagcaatta caacaataag caaaagacgt ataagcaaaa
      481 taaatgattc tgatcctact ggaacgataa taatttcaga agatgacatg aatgaaatcg
      541 aaaagcaatt aaagataaag ttcttttcta attgaattgt aatattacat ctgatataat
      601 tggtttacaa attcccggta accattccgg cttcattctc ttgaagaatg acatattctc
      661 agcgttttag ctgaaggtca gatgatacgt catctggcct ctttttttat ataaatgccc
      721 tttaagtgaa atatacaaca atctgcaacc aatatagaca tcacaaaaca cacaataaat
      781 tacacaatgt gttataagcc acatgtcact attatcgtag ctagctgcgt cactcaaaac
      841 gaatacggaa cgcgttggtt attccaatca taatattact ctgcaaatac acagccctgt
      901 aaccgagtaa atggatagag atttgaacaa atgaaaacaa tcaactaatg gaaaggataa
      961 aatattatgc acaacaaaaa tgaattcaac aaaaaactga aagattttat aggtagcgat
     1021 aaacggatgg ctttagtaaa gggttatatt aacgagtata aattaacaac tgttttaaga
     1081 gctttaaata attccgatta cacaaaaggg acgatacaca ctcgttcaat aggcaaccta
     1141 aaacaaatag tagataatcg aattattcct aaaaatataa ctcaaaacac aatcttcaaa
     1201 ttgagtaatt taaccttaga ggttaacctc tatgaaagaa acacaattta tcacggagac
     1261 tttgctattt actatcctgt agaatctgct ctaataatag aaacagatac tcctaggcta
     1321 attaatcata taaataacaa cccagtccaa aaaatattta taataacaac aaatgactgg
     1381 agctttaata cacttgaatt agaaaaaata gtagacgaaa ctataattta tgatttaaaa
     1441 caagaagatc ctaaacaata tgaaatttta tataaaaata aacacggaaa attgccttat
     1501 tgatgaatta aaacgcaaac ttagaacacc ttaccccttt tccgtcctta ttgtaatttt
     1561 aaatcatcaa gaggctggga cataaatccc
//
LOCUS       DATTQF010000238         1531 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.243, whole genome shotgun sequence.
ACCESSION   DATTQF010000238 DATTQF010000000
VERSION     DATTQF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.243"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>1531
                     /locus_tag="VB241_002688"
     rRNA            <1..>1531
                     /locus_tag="VB241_002688"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          430 a          305 c          456 g          340 t
ORIGIN      
        1 ggtgaaaagc accccggaag gggagtgaaa tagaacctga aaccgtgtgc ttacaagtag
       61 tcagagcccg ttaatgggtg atggcgtgcc ttttgtagaa tgaaccggcg agttacgatt
      121 tgatgcaagg ttaagcagta aatgtggagc cgtagcgaaa gcgagtctga atagggcgtt
      181 tagtatttgg tcgtagaccc gaaaccaggt gatctaccct tggtcaggtt gaagttcagg
      241 taacactgaa tggaggaccg aaccgactta cgttgaaaag tgagcggatg aactgagggt
      301 agcggagaaa ttccaatcga acctggagat agctggttct ctccgaaata gctttagggc
      361 tagcctcaag tgatgattat tggaggtaga gcactgtttg gacgaggggc ccctctcggg
      421 ttaccgaatt cagacaaact ccgaatgcca attaatttaa cttgggagtc agaacatggg
      481 tgataaggtc cgtgttcgaa agggaaacag cccagaccac cagctaaggt cccaaaatat
      541 atgttaagtg gaaaaggatg tggcgttgcc cagacaacta ggatgttggc ttagaagcag
      601 ccatcattta aagagtgcgt aatagctcac tagtcgagtg acactgcgcc gaaaatgtac
      661 cggggctaaa catattaccg aagctgtgga ttgtcctttg gacaatggta ggagagcgtt
      721 ctaagggcgt tgaagcatga tcgtaaggac atgtggagcg cttagaagtg agaatgccgg
      781 tgtgagtagc gaaagacggg tgagaatccc gtccaccgat tgactaaggt ttccagagga
      841 aggctcgtcc gctctgggtt agtcgggtcc taagctgagg ccgacaggcg taggcgatgg
      901 ataacaggtt gatattcctg taccacctat aatcgtttta atcgatgggg ggacgcagta
      961 ggataggcga agcgtgcgat tggattgcac gtctaagcag taaggctgag tattaggcaa
     1021 atccggtact cattaaggct gagctgtgat ggggagaaga cattgtgtct tcgagtcgtt
     1081 gatttcacac tgccgagaaa agcctctaga tagaaaatag gtgcccgtac cgcaaaccga
     1141 cacaggtagt caagatgaga attctaaggt gagcgagcga actctcgtta aggaactcgg
     1201 caaaatgacc ccgtaacttc gggagaaggg gtgctcttta gggttaacgc ccagaagagc
     1261 cgcagtgaat aggcccaagc gactgtttat caaaaacaca ggtctctgct aaaccgtaag
     1321 gtgatgtata ggggctgacg cctgcccggt gctggaaggt taagaggagt ggttagcttc
     1381 tgcgaagcta cgaatcgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
     1441 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga tttgggcact
     1501 gtctcaacga gagactcggt gaaatcatag t
//
LOCUS       DATTQF010000239         1522 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.244, whole genome shotgun sequence.
ACCESSION   DATTQF010000239 DATTQF010000000
VERSION     DATTQF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.244"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..1335)
                     /gene="cls"
                     /locus_tag="VB241_002689"
     CDS             complement(<1..1335)
                     /gene="cls"
                     /locus_tag="VB241_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP1245900.1"
                     /translation="MQFSFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIIIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVY"
BASE COUNT          528 a          270 c          188 g          536 t
ORIGIN      
        1 atatacaaag gcatttactt caaaatttaa ttcaaaactt ctaaagtcca tatttgctgt
       61 gcccactgat acgatttcat catcaattaa gcacatttta gaatgtataa atccattttc
      121 atacgtataa attttaacac cacttgataa taagtcagag gcatttgaaa atgtcgccca
      181 ataaactaaa ggatgatctg gcttacatgg aatcattaaa tgtacatcta cacctgattt
      241 agctgcaatt ttaatggcat ttatatatga attatccgga atgaaataag gcgattgcaa
      301 atataccgac ttcttagcgc tcataatcat ttttgtataa ccatattcaa tttgatgcca
      361 gtcactagct gggccacttg cagctatttg aattggtgaa ttacccaatg gtccgttctt
      421 tttagggaaa tacttaacat catattcaaa ttgaggtcga tgtgcttgcg aattccagtc
      481 taaaataaag cgcaattgta atgcatcaac tgcatctcct tgaattcgta aatgtgtatc
      541 tctccaatat cctagtttac ctaaaccaag gtattcatct ccaatgttaa atcctccgac
      601 ataacctaat tgaccatcaa taataatgat ttttctatga tttctgttat tcatacggaa
      661 atttaataat ggtagtttag acgcaaaaaa tgcttcaact tcgccaccta atgatttaaa
      721 gtgatcaaaa tttgccatct taacattttt agaaccaaca tcatcatata atatttttac
      781 ttctaaacct tgtttcaatt tttcttctaa agcatgtaaa attcttttac ctaaaccatc
      841 taaagcgaaa gtatagtatt ctaaatggat atattctttt gcatttttaa tatcttttaa
      901 aacttgatcg tataaatcat taccgtcaat gaaatgatca actttattat tttcagttaa
      961 aaaaccatct tgatccatca aaagcatacg tactaaatca tgatgttttt gaacttgttt
     1021 gttatcagta ccataattac ctttatcaaa gctttctatt tgttgtttca aaagtccatc
     1081 gaaatccgtt aacacgttac cattgttttt attcaatttg cgtgccgaaa cggttctacc
     1141 aaaaaacaag taaagaataa aaccaatcaa gggtaagaca aaaagtacaa atagccatgc
     1201 ccaagttgaa ctcgctgtac gcctatttct ttctaaaaag ataataataa aagctaatac
     1261 taaattaatg atgaatccaa tggctaaaat aatagtaaat aacgttccta aatcgtttga
     1321 aaatgaaaat tgcatagttt atttctccta taaagaaagg cacaaaaaac ttttgtacct
     1381 ttcgttccag attatttatt tacagataga gttaatattg cattaaactc ttcttcatta
     1441 tttggaaatg ttctttcctc aatttctttt attgtaatat ttactaattt caaatttgca
     1501 gcttcttcag aacctaaaaa tt
//
LOCUS       DATTQF010000240         1466 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.245, whole genome shotgun sequence.
ACCESSION   DATTQF010000240 DATTQF010000000
VERSION     DATTQF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.245"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(202..375)
                     /locus_tag="VB241_002690"
     CDS             complement(202..375)
                     /locus_tag="VB241_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245901.1"
                     /translation="MKKFFMFFVCLLTVFLSFNFVNSILFDGSFVFLESFIYSLVFTL
                     VFLGLEGDKEKKK"
     gene            complement(929..1244)
                     /locus_tag="VB241_002691"
                     /pseudo
     CDS             complement(929..1244)
                     /locus_tag="VB241_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1237..>1466)
                     /locus_tag="VB241_002692"
     CDS             complement(1237..>1466)
                     /locus_tag="VB241_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEP1245902.1"
                     /translation="RHEDMHYMLEDIEDEILTSLKRLKKAIHDRGVKGQKETIKSMQA
                     YLMQTILTELEEIHALYMRRKNMKQYNIFNQ"
BASE COUNT          507 a          255 c          182 g          522 t
ORIGIN      
        1 aaacttctaa agattattag atagcaaatc tatattgctt tattccaatt gctttattga
       61 cgttgagcct cggaaccctt aacaatccct aaacttgtcg aatagtcggc ttaatagctt
      121 atgctatgcc gacattcgtc ttcaattttg gttaagggtt cttctcaata tcaataaatt
      181 ttctcggcat aaatgcgttt cctacttctt tttctcctta tccccctcta atcctaaaaa
      241 aacgagtgta aaaaccaatg aataaataaa actttctaaa aagacaaaag aaccgtcaaa
      301 taaaatcgaa ttaacaaaat taaaacttaa aaacacagtt aataaacaaa caaaaaacat
      361 aaaaaacttt ttcataatag actcctccaa ttttttaaca aactttatca caagacttac
      421 taaaataatg aatgccccta ttttgaattt taaaggggga ttttaaagat taaaagataa
      481 atcgcgtagt tttctatcta aaacttatat aagctcttaa aacgaaaata cgagccaaat
      541 aaatatatgg taatttcaca aacaaaaatt tcagcaaaac cagtgactaa ttttttaaca
      601 ctgcccattt acatgcaaat taaaaatgag cataaaattc tttaaaactt tcccatgttt
      661 ttctaaattc ctgcagggcg ttttttatca aaaatcgata aatttcaatt tttctctaaa
      721 aactccgtaa gagcataagg gaaatttgtc cccatcaatg aatgggggat ctggcaaagc
      781 cagatatttc aaggcttcga aaatgttttt gtgtgaggtc ataaaaagct gtagtgtcac
      841 acttttgtga cactacatac agcatttgtc acaaaaataa gacagcactt ttttacagaa
      901 aaacaataaa aaaagacatc agttatcact aatgtcttaa ataataattt tgcttttcga
      961 aattccaaat tgcggtaatt gaatgtgctt tttttcttcg tctgtcgttt tatcttcatc
     1021 acttgtatta atcaaattac catcttcggc atcgtctaag tttaaaattt tatgcttttg
     1081 tttaagcaat ccaccataac ttaacatacg ttttcgatac aaaccttttt ctaaatcact
     1141 tacaatttct tgattttttc gtcgtcatca gttaaaaagt cagatgattt aacggaatat
     1201 tttgatgtct cttcgagtgt tataaaatca tttgaattat tgattaaaga tattatattg
     1261 tttcatgttt ttacggcgca tataaagggc atgtatttct tcaagttcag ttagaattgt
     1321 ttgcattaaa taagcttgca ttgactttat tgtttccttt tggcctttaa ctcccctatc
     1381 atgtatagct tttttaagtc tttttaaaga agttaatatt tcatcctcaa tgtcctctaa
     1441 catgtaatgc atatcttcat gccttg
//
LOCUS       DATTQF010000241         1346 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.246, whole genome shotgun sequence.
ACCESSION   DATTQF010000241 DATTQF010000000
VERSION     DATTQF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.246"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            41..640
                     /locus_tag="VB241_002693"
     CDS             41..640
                     /locus_tag="VB241_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HEP1245903.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            665..817
                     /locus_tag="VB241_002694"
     CDS             665..817
                     /locus_tag="VB241_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245904.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT          509 a          185 c          238 g          414 t
ORIGIN      
        1 gtttttatgt aaaatgcaaa ctaagaggag tgtagaaatc atggcaatga actttaaagt
       61 ctttgacaat agtcaacttg tagcagaata tgctgctgat attattagaa agcaatttaa
      121 caataatcct actacaattg caggttttca tttagatact gatcaagcgc cagtactaga
      181 tgaattaaag aaaaatattg aaaaacatgc tgttgatttt agccaaataa atattttaga
      241 ttatgacgat aaaaaatcat atttcgaagc gttaggtgta ccagcaggtc aagtttatcc
      301 aattgcttat gaaaaagatg caatcgaatt aatcgctgat aagattaaaa ctaaagaaaa
      361 taaagggaaa ttaacattac aagttgtttc tatcgatgag caaggtaagt taaatgttag
      421 tattcgtcaa ggactaatgg aagcaagaga aattttctta gtagtgactg gtgctaataa
      481 acgagatgta gttgaaaaat tatatcaaga aaatggtaaa acaagctttg aaccagccga
      541 tttaaaagca catagaatgg taaatgttat tcttgataaa gaagcggctg caggtttacc
      601 cgaagatgtt aaagcttact ttacgtcacg ctttgcttaa ttaaaaatct gtgaggtatt
      661 gattatgtct aaagacaaag atccaaaatt aaattatcat gaagaagaaa acagtatggt
      721 aactgatttt gaagatttaa aagaattagg taaagaaatg gaacaaattt ctgatcaaaa
      781 tgatcaagaa aagaattctg aagaagacag tcagtaaagt attataattg tgtaatatac
      841 agctatttat catgtaaaca atgaatagta tagaacctat atcaaatagg attgataatc
      901 ttgaatatga agttaggtag cattttgtta cctagctttt ttattatatt cacagaagct
      961 gactttccgc cagcttctat gttggacccg ttccccaact tgcaatgtct gtagaaattg
     1021 gaaatccgat ttcactatgt tggggtcgtt ccccaacttg caatgtcagt agaaattgga
     1081 aatccaattc cactgtgttg tggccccgcc agcttgcatg aaatagtaag ctgacatgcc
     1141 gaagcttcaa tgttggggac tacagtcaac tactgccaat ataactttgt agagcataga
     1201 atattgattt gtgtactaga catagtaata tatcaaaaat tcaattttaa attttaaaac
     1261 accatttaaa cagaattgat aattaattac tccagacata attgaaagaa gtcatctatc
     1321 agtaatattt ttaatgaaat cattaa
//
LOCUS       DATTQF010000242         1345 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.247, whole genome shotgun sequence.
ACCESSION   DATTQF010000242 DATTQF010000000
VERSION     DATTQF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.247"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            9..1127
                     /locus_tag="VB241_002695"
     CDS             9..1127
                     /locus_tag="VB241_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP1245905.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEAVVKTSDTSLKLDNKSAISNGNEINQD
                     MKISNTPKNSNQGNNLVVNNNEPTKEIKIANLEAQNSNQKKVNKGTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVPSNNKSNSNNSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            1155..>1345
                     /locus_tag="VB241_002696"
     CDS             1155..>1345
                     /locus_tag="VB241_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEP1245906.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIF"
BASE COUNT          567 a          164 c          228 g          386 t
ORIGIN      
        1 gtttcgtcat gaataaacaa caaagtaaag tacgctattc aattagaaaa gttagtattg
       61 gaattttgtc aatttcaata ggtatgtttt tggcattggg tatgtcgaac aaagcatatg
      121 cagatgaaat tgataaatct aaagatttta caagagggta tgagcaaaat gtattcgcga
      181 aatcagagtt aaatgctaat aaaaatacga caaaagacaa aataaaaaat gaagctgttg
      241 ttaaaacatc ggacacaagt ttaaagttag acaacaaatc agcaatttca aatggaaatg
      301 aaattaatca agatatgaag atttcaaata ctccgaaaaa ctcaaaccaa ggtaacaatc
      361 tagttgttaa taacaatgaa cctactaaag aaattaaaat tgcaaacttg gaagctcaaa
      421 attctaatca gaagaaagtg aataaaggta ctaataatta ctttggttac tacagtttta
      481 gagaagctcc aaaaacacaa atctatactg taaaaaaagg tgacacactt agtgctatag
      541 cattaaaata caaaactaca gtttcaaata ttcaaaatac aaataatata gcaaatccta
      601 atttaatatt tattggtcaa aaattaaaag tgccaatgac accattagta gaaccaaaac
      661 caaaaacagt gccttcaaat aataaaagta atagtaataa cagtacatta aattatttga
      721 aaacattaga gaatagagga tgggatttcg acggtagtta tggatggcaa tgtttcgatt
      781 tagtcaatgt gtattggaat catctttatg gtcatggatt aaaaggatat ggagctaaag
      841 atataccata tgcaaataat tttaatagtg aagctaaaat ttatcacaac acaccaactt
      901 tcaaagctga acctggggac ttagtggttt ttagtggaag atttggtgga ggatatggtc
      961 atacagctat tgtcttaaat ggtgattatg atggaaaatt aatgaagttc caaagtttag
     1021 atcaaaactg gaataatggt ggatggcgta aagcagaggt tgcacataaa gttgttcata
     1081 attatgaaaa tgatatgatt tttattagac catttaaaaa agcataattt aaatcgaagg
     1141 caggacattg aaatatgaaa ttttcaactt taagtgaaga agaatttacc aactacacca
     1201 aaaagcactt caaacattat acacagtcta tagaattata taattataga aataaaataa
     1261 atcatgaagc acatattgtg ggagtgaaga atgataaaaa tgaagttata gctgcatgtt
     1321 tattaacaga ggcacgtatt tttaa
//
LOCUS       DATTQF010000243         1287 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.248, whole genome shotgun sequence.
ACCESSION   DATTQF010000243 DATTQF010000000
VERSION     DATTQF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.248"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            33..443
                     /locus_tag="VB241_002697"
     CDS             33..443
                     /locus_tag="VB241_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HEP1245907.1"
                     /translation="MYFTGRTYYEKNITKVIIASTVIATGLLTHSNDAKAFFSYEWKG
                     LEIAKKLADQAKKDDERATKLIDEGFKGETVNDLYIIVKKLSKGDVKKAVVKMRNGGP
                     KDFYTFDLTRPLEENRKNIPLVKNGEIDSIYWDQ"
     gene            409..492
                     /locus_tag="VB241_002698"
     CDS             409..492
                     /locus_tag="VB241_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245908.1"
                     /translation="MVKSTRSIGINKHTELNSCTPNVGKQC"
     gene            complement(791..1108)
                     /locus_tag="VB241_002699"
     CDS             complement(791..1108)
                     /locus_tag="VB241_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HEP1245909.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKAEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
BASE COUNT          453 a          206 c          177 g          451 t
ORIGIN      
        1 aaatagcagt gtactcgcat tagtaagtac aaatctattt tacagggaga acttattatg
       61 aaaaaaatat tacaaaagta attattgcat ctacagtcat cgctacaggt cttctaactc
      121 attcaaatga tgctaaagct ttctttagtt atgaatggaa aggattagaa atcgctaaaa
      181 agttagcaga tcaagctaaa aaagatgatg aacgtgctac taaattaatt gacgaaggtt
      241 ttaaaggtga aactgttaat gacctataca tcattgtcaa aaaattaagc aaaggcgatg
      301 tgaaaaaagc tgtcgttaaa atgagaaatg gaggacctaa agatttctat acttttgact
      361 taactcgtcc tttagaagaa aacaggaaaa atattccctt agttaaaaat ggtgaaatcg
      421 actcgatcta ttgggatcaa taaacacact gaattaaata gttgcacgcc aaacgttgga
      481 aaacaatgct aacgttttgg cgtgcttttt tatatgctgc aactcccaat ttatttatca
      541 tctttacgac tgtttaataa agcacgtatg attaacacaa tacctctaaa tccgcgaaaa
      601 ttctaacgat tgttaataca tatgcttggc gttatcccgt cattcaaaac acctatcaca
      661 tacattttca aaggattttc taagatttac cccaatatac tcatcaagta aaatacatac
      721 aatgtttaaa taactttcat cagattacac atagtaaaaa agccacactc tatagcatgg
      781 cttctatcaa ttattttaaa gtattatatt ttaaaactag atcgatttgt ctttgtaatt
      841 ttttcttttc atagctgtgt tggaaatgaa ttaagttaac agctctttgt gctttacggt
      901 gtgttgcaac agttcttgta cgtttgaaca agtttacagc tttttgtgca tccacaactt
      961 ttttatttac ttgtttttta aagtttgttt gatactcgtt atatttttca gccttagttt
     1021 gtgcatgaga ttcaccggca aatgttgata ccgttaaact aatagcagct atgcttaaaa
     1081 ttgatttccc aataaaattc tttttcataa ttaataacac ctatcctcaa atatttattt
     1141 tgtatatatt tataataata gacagaagca aaaaactaca ttaactattt attaactaaa
     1201 cattaatatt tgtgtcacta catttctgaa tttcaagaat tttgctactc catattttaa
     1261 ccatcaaatt attgatataa tttatgc
//
LOCUS       DATTQF010000244         1268 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.249, whole genome shotgun sequence.
ACCESSION   DATTQF010000244 DATTQF010000000
VERSION     DATTQF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.249"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            5..1249
                     /locus_tag="VB241_002700"
     CDS             5..1249
                     /locus_tag="VB241_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEP1245910.1"
                     /translation="MQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIP
                     VVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNLFKPGAGLDPTK
                     LPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELLPIIFFAVFFGL
                     GLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLL
                     KLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTSSSEAVLPVMMK
                     KMENFGSPKDITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLTLSEQIVLMLTL
                     MITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNALST
                     IVIAKWENVYDKAKGQEYLKSI"
BASE COUNT          409 a          185 c          230 g          444 t
ORIGIN      
        1 acctatgcaa gtagttattg cattagtgtt aggtgttgtc gtaggacttt tattatatgg
       61 acaagaaaac gtagcaaatt acattaaacc atttggtgac gtatttttaa atttaattaa
      121 aatgatcgtt ataccagttg tattttgctc actagcgctt tctatttcga acgttgggga
      181 atcgaaaact gtagggcgtt atggctggaa aacaatttta tactttgaaa ttattacaac
      241 aatcgcaata ggtttaggga ttatcttcgg taacctattt aaaccaggtg ctggattaga
      301 cccaacaaaa ttacctaaag gtgatatttc taaatatcaa tcaactgcac atgcagcaga
      361 acaatctaca tatggaaatc attttattga taccattgta catattattc cgacaaactt
      421 ttttgaagct ttaaataagg gtgaattatt acctattatc tttttcgcag tattctttgg
      481 attaggatta gctgcagtag gtaaaaaagc agaaccagtt aaagaatttt taagcggatc
      541 gcttgaagct gtgttctgga tgattaataa aattttaaaa ttagcaccac ttggagtgtt
      601 tgcattcatt tgtactacaa ttattacatt tggtgcatca gcattattac cactattaaa
      661 attagtatta gttgttgtct ttgcaatggt gttctttgta ttcgctatac taggactagt
      721 tgcatggatg tgtggtatta atatcatgaa tattattaga atcttgaaaa gtgaattgct
      781 tttagcattt tctacatcaa gttcggaagc tgttcttcct gtaatgatga agaaaatgga
      841 aaactttggt tcaccaaaag atattacatc ctttgttata ccgattggct atacgtttaa
      901 cttagacgga tcagcacttt atcaatctat tgcagcatta ttcgttgcac agatgtatgg
      961 aatgcactta acattatcag agcaaattgt gttgatgtta acattaatga ttacatctaa
     1021 aggtatggcg gcagtaccag gaacttcaat cgttgtttta ttaacaacat taggcgccat
     1081 gggcttaccg gcacaaggtt tagcattaat tattggtgtt gaccgtatct tagatatggt
     1141 acgtacatgt gtaaacgtta ttggtaacgc attatcaaca attgttattg ctaaatggga
     1201 aaacgtatat gataaagcaa aaggtcaaga atatttaaaa tcaatttaaa aaatactatc
     1261 tgacattt
//
LOCUS       DATTQF010000245         1240 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.250, whole genome shotgun sequence.
ACCESSION   DATTQF010000245 DATTQF010000000
VERSION     DATTQF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.250"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            338..984
                     /locus_tag="VB241_002701"
                     /pseudo
     CDS             338..984
                     /locus_tag="VB241_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190211.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            995..1189
                     /locus_tag="VB241_002702"
     CDS             995..1189
                     /locus_tag="VB241_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245911.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          536 a          125 c          222 g          357 t
ORIGIN      
        1 taagtaagac agaacatttt aaagaaaaag taatgaaaaa aatgatagca aacttattaa
       61 tagaaaaatc tctagatgta attagtacaa aagatattga aaggttatta aaagagttat
      121 acaataacga taatataata taaagaaata ggtgattaaa aggtgacaat gatgttagac
      181 ggtaagttat ctaaaaaaga attgctcagg ttgttaactg aaaaaaacga tgaaaaaaat
      241 aagggaaaag agaaacaatc taaatagcat cctttctaca caaaaatata gaaagtggtg
      301 ctatttttat atgctttttt aactatttac tgaaatagca gaatcacaaa agatagctta
      361 tcttaaagat ttaggcgaag atggcgaata taaatatgtt gccaaaatag atagtaaaac
      421 atctaaatta tgtcattcac ttaacggaaa aatatttaaa gttaaagata tgataccagg
      481 tgtgaatgcg ccacctatgc atccttggtg tagaagtacc acagtgccac atgtcggcaa
      541 ttggcgagac aagttcttta aagagcgtga aggtaagtat caagtagaag taaaagaagc
      601 aaaattacag gaaaaagcta aaaaccagat gaaagaaatg attgaaagtg ataaaataaa
      661 aatataaata aattgtggga aataaaatag atatatgtta ggtcatcact tatataatga
      721 aaataaaaaa agagccattt taaataaaaa gaaattgcct agctatacaa tactttctat
      781 agatctattg agtgaattgt taagagaaaa atgtcaacag gcaatctaat attaagtgat
      841 gagcgatttg atttgaaaga tattattaat tttaatcaaa ttattggaaa aatacatatc
      901 ggaaatgtgt atattgaaac cataaaggaa aaagtgcatt attcgaagac aggtgctcat
      961 atagtacctt atattgataa gtaggtgaaa agtattgaga attgaagatg catatcgtaa
     1021 agatgttatt atcacgcttt tgaataatga agaatacgaa gggtttgtaa ctgactatga
     1081 aaatgaattt gagagtgaaa caggaaatct tgttgtagat atacagaccg attttgctgt
     1141 ttattcgttt gatgaaactg agataaaaag tattagatta ttaaaataat ttctaaatag
     1201 catcctttct acacagataa agagaaagcg gtgctatttt
//
LOCUS       DATTQF010000246         1236 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.251, whole genome shotgun sequence.
ACCESSION   DATTQF010000246 DATTQF010000000
VERSION     DATTQF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.251"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            202..312
                     /locus_tag="VB241_002703"
     CDS             202..312
                     /locus_tag="VB241_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245912.1"
                     /translation="MKITIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            705..890
                     /locus_tag="VB241_002704"
     CDS             705..890
                     /locus_tag="VB241_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245913.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            complement(1102..>1236)
                     /locus_tag="VB241_002705"
     CDS             complement(1102..>1236)
                     /locus_tag="VB241_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEP1245914.1"
                     /translation="KTDLAKVNQEQLNYALDSINYRPRKCLNWKFPYEVLCDELLHLN
                     "
BASE COUNT          527 a          113 c          194 g          402 t
ORIGIN      
        1 agaatcgtgt ggtaaacata atttatatag attataagga ggtgaaaatg gtcgaggaag
       61 taaaaacgat aaaatagttt gaatgttttg attttaattc tgataggact attgaatttg
      121 caaactaata agaatgtgag atttgattta atacgaagtc atattctttg aataaaatat
      181 tactttctca ccattagtaa aatgaagata actattaaag tgcgaaaaac aaataaaaaa
      241 gagaaaatag aatttttaat agggacgttt ataattattt tagtaatatt agggttcaaa
      301 attatgaaat aaatgtatag tatttgagaa gtctatatga ctccggaaaa attaaagtct
      361 aaagatatct ctatatctca aatttaatag actactggag ttagtagttt gaatattgaa
      421 agacactaat ctaataaaaa tacataaaag gttaaacatt gaagtttagt aaaagacgta
      481 aggtatacaa gacaaatcgt ttgtctttga taaagtaatt gatgatgaaa agtcgttaat
      541 atgtagaaac aaaaattgaa aatatgaaat ttaactatta tgaagtgatg atagccatta
      601 atgaatagaa aaataataaa atcatttata aataaaaaat acgttgagtt cacagaattt
      661 cgaaaaaaag tagtaaacat tgtacagata tggagatgaa aaaaatgtca gaatacaaga
      721 aaaagataat tgaattaatt gaaagtaatt taacaggata tgaaatttct aaaaaaactg
      781 gagtttctca atatgtactt tcacaattaa gacagggcaa acgcgaagta gataatctaa
      841 ccctgaatac aacagaaaaa ttatatgaat atgccaataa agttttgtaa tttaactact
      901 gtataaatta atcaagctat gtttatttga tttaactatt aataaaaatc atatggtgaa
      961 tggatattat aataattaaa atacaaaaat agtagattcc aatttgtgaa aaaggagtat
     1021 gtcgtttcat ttagaattta ttgcacgaaa ttagcatcga ataagggaaa ataagtgtta
     1081 agttttaaat gagttgattt gtcaatttaa gtgcaacagt tcatcacata aaacttcata
     1141 aggaaacttc caatttaaac actttcttgg tcgataatta attgagtcta atgcatagtt
     1201 aagttgctct tgatttactt tagctaagtc tgtttt
//
LOCUS       DATTQF010000247         1231 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.252, whole genome shotgun sequence.
ACCESSION   DATTQF010000247 DATTQF010000000
VERSION     DATTQF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.252"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..288
                     /locus_tag="VB241_002706"
     CDS             <1..288
                     /locus_tag="VB241_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEP1245915.1"
                     /translation="MFGGYDELMEPVCNTFTAKEPFNQLGGYPYFDQIDPRTNDQELK
                     MYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTDLEAMKFDDYMYSWDCS"
     gene            879..>1231
                     /locus_tag="VB241_002707"
     CDS             879..>1231
                     /locus_tag="VB241_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEP1245916.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSNDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPFLPN"
BASE COUNT          400 a          166 c          239 g          426 t
ORIGIN      
        1 atgtttggtg gttatgatga attaatggaa cctgtgtgta acacatttac tgctaaggaa
       61 ccatttaatc aacttggtgg ttatccatat tttgaccaaa tagatccaag aacgaacgat
      121 caagaactga aaatgtatga tagagtctta ctgcaaattg attctacaag agatggtaat
      181 tcttcgatta tatggggtga tttaggtatt gccaatatct tagtaaaatc tactgacctt
      241 gaggctatga agtttgatga ttacatgtat tcatgggatt gcagctaaga gaatcataaa
      301 aatatattac tttaataagt gtagtatctg tagatcaatc tagggagtgg gacagaaatg
      361 ataaagaacc actagtgatt tataatgtag tggttctttt tcataagcca cagctaatgt
      421 gtacttaaaa ataggaatac atgagtaaaa ctcatgcata agaaatacta atttctaaag
      481 aaaaagtata tctttatgtt tgggcccacc ccaacttgca cattgcttgt aagctgactt
      541 accgtcagct tctgtgttgg ggcccacccc aacttgcatt gcttgtaagc tgactttccg
      601 tcagcttcta tgttggggcc ccgctataat tgaaaaaggc ttgttatagg tgtattttct
      661 tttggttaac tattggtaat atatcattgt agattttagg atgttgattt atggtttagc
      721 ctttgtctgc ttatgttaaa tgtcccagct agtcaaacat tgattttgta attattatat
      781 taagctagtt taaatttttt gtgatttatg ttttattcat accaattttg tgaaatgata
      841 cagtgacaga tgtttattga ttttaaaggg tgatgatgat ggctattgta aataaagtga
      901 taattgttga agggaaatct gataaaaaaa gggtgcaaca ggttattgca gaaccagtca
      961 atattatttg tactcatgga acaatgagta tagataagct tgatgatatg atagaatcac
     1021 tatatgataa acaagttttt gtattagccg attctaatga cgaaggagat cgaattagaa
     1081 attggtttaa acgttatttg agtgaaagtg aacatatatt tattgataaa acttactgtc
     1141 aagttgcgaa ttgccccaaa caatatttgg cgcatgtact ttcaaaacat ggctttactt
     1201 gtaagaaaga aacacctttc ttaccgaata t
//
LOCUS       DATTQF010000248         1227 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.253, whole genome shotgun sequence.
ACCESSION   DATTQF010000248 DATTQF010000000
VERSION     DATTQF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.253"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..1046
                     /locus_tag="VB241_002708"
     CDS             <1..1046
                     /locus_tag="VB241_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP1245917.1"
                     /translation="IPNHIFKKILTNDIEESLQTNYEEPKGNLKLRIEIVKYMKRRGV
                     DCNINNICITSGAVQGLKLIADGLLIPQSKIIIETPSYINSIRTWHNIRAKIIPLSIN
                     YIKQNINNIFKLNSDYRHSIFYCIPTLHNPTQNTYSKEEKQKIIDQCHKEGIPIVEDS
                     IYSDLWFSPNQQISMKALKNSDNVLYLGSLSKTVSPGLRIGWIIADEKVIHHLADLKM
                     QNDYGASSISQYIATTWLSNYHEDHLYKLKIELKLRKSIFIKSLKKHLSKFGYWNEPQ
                     GSFYIWFKLSVPVNIKLLFNEAIKENILIHPGEIYDINSKSYIRFSYSYIDKEEIDQS
                     LKKLSKIINRIRT"
BASE COUNT          498 a          171 c          159 g          399 t
ORIGIN      
        1 taattccaaa ccatatattt aaaaaaatat taactaatga tattgaagaa tctttacaaa
       61 ctaactatga agaacctaaa ggaaatctaa agttaaggat agaaatagta aagtatatga
      121 agcgaagagg agttgattgt aatatcaata atatatgtat tacatccggt gctgtacaag
      181 gtttaaaact aattgcagac ggactgttaa ttccacaatc taaaattata attgaaacac
      241 catcatacat taattccata cgtacttggc ataatatcag agcgaaaatt attcctttgt
      301 ccatcaatta catcaaacag aatattaaca atatctttaa attaaatagt gattataggc
      361 acagtatttt ttactgtata cctacgctgc ataaccctac tcaaaatact tatagtaaag
      421 aagagaagca aaaaattata gatcaatgtc ataaagaagg tattccaatt gtggaagata
      481 gtatatactc tgatttatgg ttcagcccta accaacaaat ttctatgaaa gctttaaaaa
      541 atagtgataa tgttttatat ttaggtagtt tatctaaaac tgtaagtccc ggtttacgta
      601 ttggttggat tatagcagat gaaaaagtaa ttcatcattt agccgattta aaaatgcaaa
      661 atgattatgg tgcaagttct atatcacagt atattgccac aacgtggtta agtaattatc
      721 atgaagatca tttatataaa ttaaaaatcg aactaaaatt acgaaaatct atttttataa
      781 agtcattaaa gaaacatcta tctaagtttg gatattggaa tgaaccccaa ggctcatttt
      841 atatctggtt taagctatct gttccagtaa atataaagct cttatttaac gaagcaataa
      901 aagaaaatat actaatacac ccaggagaaa tatatgacat taactctaaa agctatatcc
      961 gcttttcata ctcatatatc gacaaggaag aaattgacca aagcttaaaa aaattaagta
     1021 aaattataaa tagaatacga acttagttaa catttatcac cctttatata attcatttta
     1081 tacatgcgtg ttataatatt aagcacaata cgcattacat ttcataaaaa acctaaaatg
     1141 cattttgtta taagatattg ggatattata cacatttaca aatattgaca atatgatcta
     1201 tatcatcaaa tattatttca ttgtatg
//
LOCUS       DATTQF010000249         1195 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.254, whole genome shotgun sequence.
ACCESSION   DATTQF010000249 DATTQF010000000
VERSION     DATTQF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.254"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>1195
                     /locus_tag="VB241_002709"
     CDS             <1..>1195
                     /locus_tag="VB241_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1245918.1"
                     /translation="KTGNGNSKITSGLYWIAPETFANFDSRSIWYIFDTYKLPKELIN
                     EQLKPKEKLTGRHYLELINMLKQVLKCQEVEINDFIDLSYESWHYSEMINEANRNKFD
                     SNIKEIVVSENDNIIQNRSRTTKVIPYGKQEFLDEVFIDESDYDRLVQLLRRKRNVIL
                     QGPPGVGKTFLAKRLAYSLIGSKCKERIKLIQFHQSYSYEDFIMGYRPTSSGFELTTG
                     TFYDFCKKADKDRDNEYFLIIDEINRGNLSKIFGELFMLIESDKRDEELILLYSGERF
                     SVPRNIYIVGMMNTADRSLAILDYALRRRFAFYNMQPAFHTTKFKKLLKAINNTELES
                     VINCIEGLNKEIVQDDMLGNGFVIGHSYFSNIECIDKDELSNIIEFEIIPMLEEYWFD
                     EPSKIN"
BASE COUNT          447 a          125 c          219 g          404 t
ORIGIN      
        1 taaaacagga aatgggaata gtaagattac gagtggtttg tactggattg cacctgaaac
       61 ttttgctaac tttgattcac gttcaatttg gtacattttt gatacatata aactccctaa
      121 agaattgatt aatgaacaac ttaaaccaaa agaaaaacta acaggtcgac attatttaga
      181 attgataaat atgttaaagc aagttttgaa gtgccaggaa gtagagatta atgattttat
      241 tgatttaagt tatgagtctt ggcattattc agaaatgata aatgaagcta atagaaacaa
      301 atttgattct aatatcaaag aaattgttgt cagtgaaaat gacaatataa tacaaaatag
      361 aagtagaaca actaaagtta taccatatgg aaaacaagaa ttcttagatg aagtttttat
      421 tgatgaaagt gattatgata ggttagtaca acttttaaga agaaagcgaa atgtcattct
      481 acagggaccg cctggtgtag gaaaaacttt tcttgcaaaa cgtcttgctt attcattgat
      541 tggcagtaag tgtaaagaac gaattaaatt aattcaattt catcaaagtt attcatatga
      601 agattttatt atgggatata gacctactag ttcaggtttt gaactgacta cagggacgtt
      661 ctatgatttt tgtaagaaag ctgataaaga ccgcgataat gaatattttt taattattga
      721 tgaaattaat aggggtaatc tgagtaaaat ttttggtgaa ttatttatgt tgattgaaag
      781 tgataaaaga gatgaagaac taatattact ttatagcggt gagagatttt ctgtacctag
      841 aaatatttat attgtgggaa tgatgaatac agcagatcgt agtttagcaa ttttagatta
      901 tgcattacgc agacgctttg cgttttataa tatgcaaccg gcgtttcata caactaaatt
      961 caaaaaactt cttaaagcaa ttaataatac tgaattagag tcagtaatta attgtattga
     1021 aggattaaat aaagagattg ttcaagacga tatgttaggt aatggctttg ttattggtca
     1081 tagttatttt agcaatattg aatgtatcga caaagatgag ctttctaata ttatagaatt
     1141 tgagattatt cctatgttag aagaatattg gtttgatgaa ccttcaaaaa taaat
//
LOCUS       DATTQF010000250         1176 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.255, whole genome shotgun sequence.
ACCESSION   DATTQF010000250 DATTQF010000000
VERSION     DATTQF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.255"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            99..941
                     /locus_tag="VB241_002710"
     CDS             99..941
                     /locus_tag="VB241_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP1245919.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHITADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGESIDAYILRETL
                     VDNN"
     gene            complement(1065..>1176)
                     /locus_tag="VB241_002711"
     CDS             complement(1065..>1176)
                     /locus_tag="VB241_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP1245920.1"
                     /translation="RLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVER"
BASE COUNT          418 a          168 c          220 g          370 t
ORIGIN      
        1 gtggggcgtt tcatttttgt tcgcatttat gaatagtgat atgatgtaat aaacacatgc
       61 taattgtaat aacgaatata gaaagaatag gtgatgttat gaaaatcggg gatatatcta
      121 ttcattatct aaatggtggc aatacaaaaa tggatggcgg tgcaatgttt ggtgttgttc
      181 cgaagccgtt gtggtcaaag caatacaatg caaatgaacg aaatcaaatc aatttaccga
      241 cacatccaat tttgattcaa acggctcaat ataatttgat tatagatgcg ggtattggta
      301 atggtaaatt atctgaaaag caattacgta attttggtgt agatgaagaa agtcatataa
      361 ctgctgattt ggcaaattat aatttaacgc caaaggatat tgattatgta ctaatgacac
      421 atatgcattt tgatcatgct gccggtttga ctgatcaagc gggacatgca atttttgaaa
      481 atgcgattca tgttgtgcaa caagatgagt ggcatgagtt tattgcacct aatataagaa
      541 gtaaatcaac ttactgggat aagaataaag gcgattatag taacaagttg attttattcg
      601 aaaaacattt tgaaccggtt ccgggtatca agatgcaaca tagtggaggt catagctttg
      661 gccacacaat cattacgatt gaaagtcaag gagataaagc agttcatatg ggtgatatat
      721 tcccgactac tgcacataaa aatcctctat gggtaacagc atatgatgat tatcctatgc
      781 aatcaattcg tgaaaaggaa cgcatgatac catattttat ccagcaacaa tattggttct
      841 tgttttatca tgatgaaaac tactttgctg taaaatacag cgatgatggc gaaagtatag
      901 atgcatatat tttacgtgaa acattagttg ataataacta aaaaaagatg tattactaaa
      961 caaattttca agaataaaaa attgagccac atcctatctt actaattagg gtgtggctca
     1021 tttttaagtt ttacgatcca aatcaaatat ggataaaatt cgtattaacg ctctacaatg
     1081 ttaatgactt caccagtata tgcatctgca taaaaatcat aatgaatatt ttgaccattt
     1141 ttaatagttg taattccacc ttgataaact aaacgg
//
LOCUS       DATTQF010000251         1162 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.256, whole genome shotgun sequence.
ACCESSION   DATTQF010000251 DATTQF010000000
VERSION     DATTQF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.256"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            128..856
                     /locus_tag="VB241_002712"
     CDS             128..856
                     /locus_tag="VB241_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HEP1245921.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKNYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
BASE COUNT          509 a          138 c          172 g          343 t
ORIGIN      
        1 ttattacaag aattatttaa tatcatctta gaatctcaca aaatatcaac tttgtttaat
       61 tactcaaaac gataagtcgt ttagagtttt taaaataaat ttttgcgaaa taaaggagac
      121 atgttaaatg aaaaaagtaa tggggatatt attagcaagt acacttatct taggtgcttg
      181 tggacatcat caagatagtg caaaaaaaga gagcactagt cacaaaaaga aagaaaatga
      241 caatgaagaa ttaaatgaag aacttaaaga atttaaaagc aaaaaaaata tggatataaa
      301 aattaaaggc gatactattg ttagtgacaa atttgaagct aaaataaaag aaccgtttat
      361 catcaatgaa aaagatgaga aaaagaaata tatcgctttt aaaatggaaa ttactgctaa
      421 aaaagacgat aaagatttaa atccatcttc tatttctcat gactatatta atatcactca
      481 agatgataaa aatacagtaa ataaattaag agatggttat cttttaagtg ataaaaatta
      541 taaagattgg acagaacata accaagatca aattaaaaaa ggcaaaactg cacaagccat
      601 gtttatctat gagttaagag gtgatggaaa cattaattta aatgtccata aatactcaga
      661 agataaaaca gttgattcta aatcattcaa atttagtaaa cttaaaaccg aagatttttc
      721 tcatagagcg gaaacaaggg aagaagtaga aaagaaagaa aaagaatttg aagaagagta
      781 caaaaaagaa caagaacgag agaaagaaaa agaaaagcaa aaagatgacg accacagtgg
      841 tttagatgaa gtataaattt atgaatgctc gcattcagcg ttataatctt tgttgttatt
      901 attgtcatta taaattagct tacttacact cataatgaca cactaattta attgttaaat
      961 ataacaaaaa agagtagctc gcctactctt ttttgttgtt ttgaaatggt catttttgaa
     1021 atgactcgat tatagtttca cgaataccat gcaacatttt tcgtgaactc atattttaca
     1081 aatcaatatt tatcgatcaa aaataaacct atacacatag ttagctatgc ccttttaaaa
     1141 gttctatatt atattagtta aa
//
LOCUS       DATTQF010000252         1144 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.257, whole genome shotgun sequence.
ACCESSION   DATTQF010000252 DATTQF010000000
VERSION     DATTQF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.257"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            194..997
                     /locus_tag="VB241_002713"
     CDS             194..997
                     /locus_tag="VB241_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEP1245922.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
BASE COUNT          330 a          163 c          239 g          412 t
ORIGIN      
        1 ataacctatg gcattttgtc agaggggagt aacttaagaa tcatgaccgt ataaatgatt
       61 cgacacttta tcgtcattac gaagatatct tccggtaaag tgggcaatta aaattgctta
      121 gtgagacctt tgctatttat ttagcatagg tctttttgtt tgtacttaac ttatttattt
      181 aaaggagttg tacatgttaa tggatccaag tttgatctta ccttatttat gggtacttgt
      241 cgttttagta tttttagaag gcttattagc agcagataac gcaatcgtta tggctgtaat
      301 ggttaagcac ttaccacccg aacaacgtaa aaaagctttg ttttacggtt tgctaggtgc
      361 atttgtattt agatttttag cattattctt aattagtatt atcgcgaact tttggtttat
      421 tcaagctgca ggagcggttt acttaattta tatgtcaatc aaaaatctat ggcagttctt
      481 taaacaccca gaaattgaaa gtcctgaagc tggagatgat catcattatg atgagtctgg
      541 tgaagagatt aaagcaagta acaaatcatt ctggggaact gtgttgaaaa tagaatttgc
      601 agatatcgca tttgccattg attctatgct tgctgcttta gctattgctg taacacttcc
      661 taaagttggt attcactttg gtggtatgga cttaggtcag ttcgtagtca tgttcctagg
      721 tggaatgatt ggtgttattc taatgcgtta tgcagcaaca tggttcgtag agctattaaa
      781 caaatatcca ggacttgaag gtgcagcctt cgcgatcgtt ggttgggtag gtgttaagtt
      841 agttgtcatg gtattagcgc acccagacat cgctgtattg cctgagcact tcccacatgg
      901 cgtattatgg caatctattt tctggacggt actaattgga ttagtaatta tcggttggtt
      961 aggttcagtt gttaaaaata aaaaatcgca taaataaatg atgtgaagcg gacaatctta
     1021 atttagttta aggttgtcct ttttcattta attaagtgat ctatgaaaaa tggattttga
     1081 agaatatgta tcaaaagatg cgatatagta ttaagaaaat gtgcctttta tatttagcat
     1141 tttt
//
LOCUS       DATTQF010000253         1138 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.258, whole genome shotgun sequence.
ACCESSION   DATTQF010000253 DATTQF010000000
VERSION     DATTQF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.258"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..705
                     /locus_tag="VB241_002714"
     CDS             <1..705
                     /locus_tag="VB241_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1245923.1"
                     /translation="YFVAMTIYYVVILLIVFDNLKKVKDREYKKEIEEKNRYINTLIT
                     EQERHRIGQDLHDTLGHVFASLSLKSELAYKIIDSDTENAKAELLAINKLSRESLNKV
                     REIIDDIRLPSFIEEIESICKILKDADIQFTFENKELAQLLSPTKQSILVMIAREAIN
                     NVIKHANASKVHGQLRAMNEHKLQFIIQDNGEGIDSGCEIKSISQRVKHLNGTLEVES
                     NKGTKLIIEMPTGGIA"
     gene            702..>1138
                     /locus_tag="VB241_002715"
     CDS             702..>1138
                     /locus_tag="VB241_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP1245924.1"
                     /translation="MISLIIAEDQNMLRQAMIQLIKLHGDFEILADVDNGLDAIKIIE
                     AHHPDVVILDIEMSGMTGLEVLSEIRKKHLNIKVIIVTTFKRPGYFEKAVANDVDAYV
                     LKERSIEELVETIYKVYNGKKEYSASLMTSFFTDKNPLTPKEQ"
BASE COUNT          441 a          132 c          211 g          354 t
ORIGIN      
        1 tattttgttg caatgacgat ttattatgtt gttattttgt taatcgtgtt tgataattta
       61 aaaaaagtga aagatcgtga atataaaaaa gaaatagaag aaaaaaatcg gtatattaat
      121 acgttaataa ctgaacaaga gcgtcataga ataggtcaag atttacatga tacgcttggt
      181 catgtgtttg caagtttatc attaaaatca gaattagctt ataaaattat tgattctgat
      241 actgaaaatg caaaagctga attgttagcg attaataagt tgtcgcggga atcattgaac
      301 aaagtacgcg aaatcatcga tgatataaga ttaccatcat ttattgaaga aattgagagt
      361 atctgtaaaa ttttaaaaga tgctgacatt cagtttacgt ttgaaaataa agaactggca
      421 caattattga gtcctacaaa acaatccatt ttagtaatga ttgctcgtga agcgataaac
      481 aatgtgatta agcatgcaaa tgcttcaaaa gttcatggtc aattacgagc tatgaatgaa
      541 cataaattac agttcattat acaggataat ggagaaggta ttgacagcgg ctgtgaaata
      601 aagagtattt cacaacgagt aaaacattta aatggaacat tagaagttga atcgaataaa
      661 ggtactaaat taatcatcga aatgcctaca gggggaatag catgatttct ttaattattg
      721 cagaagatca aaatatgtta cgacaggcaa tgattcaatt aattaaacta catggtgatt
      781 ttgaaatttt agccgatgta gataatggtc tcgatgcaat aaaaattatt gaggcacacc
      841 atcctgatgt agttatttta gatattgaaa tgtcaggcat gactggactt gaagttttat
      901 cagaaattag aaagaagcat ttgaatatta aagtgattat tgtaacaact tttaaaaggc
      961 cggggtattt tgaaaaagct gttgcaaatg atgtggatgc atatgtttta aaagaacgtt
     1021 ctatagaaga attagtggaa acaatttata aagtgtataa cggtaagaaa gaatatagtg
     1081 cttctcttat gacttcattt ttcacagata aaaacccatt aacgccaaaa gaacaaat
//
LOCUS       DATTQF010000254         1130 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.259, whole genome shotgun sequence.
ACCESSION   DATTQF010000254 DATTQF010000000
VERSION     DATTQF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.259"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            151..>1130
                     /locus_tag="VB241_002716"
     CDS             151..>1130
                     /locus_tag="VB241_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEP1245925.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTI"
BASE COUNT          426 a          148 c          209 g          347 t
ORIGIN      
        1 tcctaaagtt ttttagctta aaagtatcat ctacagcaaa attgcaaacg acaaaattga
       61 taagtgcaat taaataaatg ttagtaagtg aatcataatt atccttgctt aagcatttgc
      121 tttgtaaggg aagtgaggag gcaactaatc gtggaagaat ttaagcaaca ttataagggt
      181 ttaattgatg aaagtttaac gtgccaagat aaagtagaat tgataaaaaa gtgtgagaaa
      241 tacactgacg aagtgattcg taaggacgtc ttgcctgaag acattgtcga tattcacaaa
      301 aactatatat tgacgttaaa cttaacgcgt gaagatgtgt tcaagacatt agatgtctta
      361 caagaaatcg ttaaaggctt tggttatagt tatcgagatt atcaaagatt ggtagataaa
      421 cttcaagttc acgataaaga gatagactta gcttctagct tacaacaaac aatgcttaaa
      481 acagatattc cacaatttga tagtattcaa attggcgtta tttcagtggc ggcacaaaaa
      541 gtaagtggag attattttaa tttaattgac cataacgatg gcacaatgag ctttgctgtt
      601 gcagatgtca ttggaaaagg tataccagct gctttagcaa tgagtatgat aaagtttggc
      661 atggattctt atggacactc acaattaccg agtgatggct taaaacgttt aaatcgtgtt
      721 gttgaaaaga atattaatca aaatatgttc gtcacaatgt tttatggttt atatgaagaa
      781 atgaaccatt tattatattg tagttcagct ggtcatgagc ctggatatat ttatcgcgct
      841 gaaaaagaag agtttgaaga aatttcagtt agaggtagag tgttaggaat cagttcacaa
      901 acacgatatc aacaacaaga aattccaata taccttgatg atttaattat cattttaacg
      961 gatggtgtga ctgaagctag aaatagtgaa ggtaccttta tagataaaca aaaactttta
     1021 gaatatatta aaaaacataa acatatgcac ccacaagata ttgttcaaat tatctatgaa
     1081 gcaattttaa aacttcaaaa cccaaataaa aaagatgata tgactatttt
//
LOCUS       DATTQF010000255         1113 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.260, whole genome shotgun sequence.
ACCESSION   DATTQF010000255 DATTQF010000000
VERSION     DATTQF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.260"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>1113
                     /locus_tag="VB241_002717"
     CDS             <1..>1113
                     /locus_tag="VB241_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISSau3 family transposase"
                     /protein_id="HEP1245926.1"
                     /translation="ANRYTFVWKKSIQNHESKLNENSKALYRDLVEEKIIPEIKEDGD
                     SDLTIEEIDLIGSHLDKEIEDLNHSIQNEDCTQIRKQTRKKRTEIKKFKKKFDDYSER
                     KSKYEEQKSILKDRNSFSKTDHDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYD
                     LFQNPTDTRTLIPFLTMIQNTFGYLPEYIVADAGYGSEQNYMAIIDDFNKTPLITYGM
                     FIKDKTRKFKSDIFNTQNWKYDELNDEFICPNNKRIGFKRYAYRNDRYGFKRDFKLYE
                     CDDCSACSLRQQCMKPNSKSNKKIMKNYNWEYFKAQINQKLSEPETKKIYSQRKIDVE
                     PVFGFMKAILGFTRMSVRGINKVKRELGFVLMALNI"
BASE COUNT          460 a          140 c          190 g          323 t
ORIGIN      
        1 atgccaatag gtatacattt gtgtggaaga aaagtattca aaatcacgaa tcgaaattga
       61 acgaaaattc aaaagcatta tatcgtgact tagttgaaga aaaaataata ccagagataa
      121 aagaagatgg agatagcgat ttaacaatag aagaaataga tttaattggt agtcatttag
      181 ataaagaaat cgaagattta aatcattcta ttcagaacga agattgtact caaattagaa
      241 aacagacccg taaaaaaaga actgagatta agaagttcaa aaagaaattt gatgattatt
      301 ccgaaagaaa aagtaaatat gaagaacaaa aatcgattct taaagataga aatagctttt
      361 ctaaaactga tcatgatgca acttttatga gaatgaagga agaccatatg aaaaatggcc
      421 aacttaagcc aggatacaat ttacaaatag cgacaaattc tcaatttgtt ttatcctatg
      481 acctatttca aaacccgaca gatactagaa ctttaattcc atttttaaca atgattcaaa
      541 ataccttcgg ttatttaccg gagtatattg tagctgatgc aggttatggt agtgagcaaa
      601 actatatggc tattatagat gattttaata aaacgccact tattacgtat ggtatgttta
      661 ttaaagataa aacgagaaag tttaaaagtg acatttttaa cactcaaaac tggaaatatg
      721 acgaacttaa tgatgaattt atatgtccta ataacaaaag aataggtttt aaaagatatg
      781 cataccgtaa tgatagatat ggttttaaac gtgacttcaa actatatgaa tgcgatgact
      841 gttcagcatg ttctttgagg caacaatgca tgaagccaaa ttcgaaatcc aataagaaaa
      901 tcatgaagaa ttataattgg gaatacttta aagcccaaat taatcaaaag ctttctgaac
      961 cagaaacgaa aaaaatctat agtcaaagaa aaattgatgt agagcctgtt tttggattta
     1021 tgaaggctat tttgggtttc actcgaatgt cagttcgagg aataaataaa gttaaacgag
     1081 agctaggttt tgtattaatg gcacttaata taa
//
LOCUS       DATTQF010000256         1104 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.261, whole genome shotgun sequence.
ACCESSION   DATTQF010000256 DATTQF010000000
VERSION     DATTQF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.261"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            1..306
                     /locus_tag="VB241_002718"
                     /pseudo
     CDS             1..306
                     /locus_tag="VB241_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            928..1104
                     /locus_tag="VB241_002719"
                     /pseudo
     CDS             928..1104
                     /locus_tag="VB241_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
BASE COUNT          459 a          103 c          181 g          361 t
ORIGIN      
        1 agagattata atatttcaga aaaaatattt gatgatttat ggatgaatct ttattacttg
       61 tttatgaatt taagggattt atttaaataa gaagatttag aaccatggac atcatgtgaa
      121 tttgacttta caagcgaagg tgatttaaac gtatcttttg attatataga ttggattaaa
      181 ttaggttttg gcccatcagg aaaggaaaac tactatatgt ataaaaagtt tggtgtttta
      241 ccagaaatgg aatatgaaat ggaagaaatt cgagcagtag agaagtatgt taaagagcaa
      301 gagtagcaga tatgttataa aaggctgtgc aaaaccaacc tcgttttaca tttgattcaa
      361 agaagaaggt aaaaaataag attatttgca acttaaaaag tcaattagct tatcggtatc
      421 catacatcat ggataaataa gttcaactaa ttaacaaatc acgatataaa tttaaaattg
      481 tcattaattg aaaaggttat tgtagtgtat tttataaagt tgatgtacat cgacttattt
      541 tttaaaactt atatcaaaaa aatatgagaa aagtatatac tattaaaatg ttttttacta
      601 taattataaa tagttaaata tttgggtata aaataaatgt gtgggagcaa tttttagaga
      661 tacatagcaa tgtaaaggta tcaaatactt attgcataat ttattatgat gacataattt
      721 tcagtagata gttgtaaatt aacactgaaa tagatgttta tattaagaag actttaatat
      781 cataattaat caaaatcttg cgtcaaacat ttaaacgaat tttaagttga taacaattgg
      841 ctaaacattg ttcaaacatc acaatgataa agcatattat cagtattgta gtgtgtggaa
      901 aatgacagcc atctaaggag aaaaatgatg aaaagaatat tggtagtatt tttaatgtta
      961 gcaattgtat tagcaggttg ttctaataaa ggtgaaaagt atcaaaaaag atattgataa
     1021 agtgtacaaa gaacagaatc aaatgaataa aattgcctcg aaagtacaaa acactattaa
     1081 aacagacatt aaacaagaag acag
//
LOCUS       DATTQF010000257         1056 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.262, whole genome shotgun sequence.
ACCESSION   DATTQF010000257 DATTQF010000000
VERSION     DATTQF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.262"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            1..1056
                     /locus_tag="VB241_002720"
                     /pseudo
     CDS             1..1056
                     /locus_tag="VB241_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          416 a          116 c          176 g          348 t
ORIGIN      
        1 cacaatgaaa gtaaaatgaa ggaagagtat attaagcgat ttaattcttt agcatctttc
       61 aatactaata ttaatatcat acctatggaa aatggaaaaa aagttaatga tttggaatat
      121 cctctattct ttatggtgac taaaaatcta tcaaaaaaca agaattaata tcaattaata
      181 gtagaaaaat tgatagagca cttaattctt taccatatgc agctagagaa caatatttta
      241 atgatttatt aatcgatgaa ttacaaagta ctaatgaaat tgaaaatgta tttagtacta
      301 aacaagagat tgcacatgcg ttaaataacc aagcatcaga ctttcttaag ttcaggggac
      361 tcgtggatca atataaagag atagaactta ataaaaaaat taaagttgat aatgtaagag
      421 acattagagc gatttatgat aaattagttt caaatgaaat taacgaacaa gataagttag
      481 atggagagct atttcgtaaa aattttgtcg gtgtgcatga tgggtcaacg aataaatata
      541 tacatgttgg gttacaacct gaaaccaaaa ttgttgaata tataggtgaa atgctaacat
      601 ttttaaaata ttttgatgcg cctcagccgt tcaaaatcat ggctagtcat tatatgtttg
      661 aatatataca tcctttttat gatggcaatg gtagagttgg aagatttata attgccaagc
      721 tcttgagtga ttattatgat aattatactg ctttgacatt ttcatatgtt ataaatagga
      781 ataaatcgaa atattacaag gcgtttatga ctgcttcaaa tcatttgaat tgtggtgatt
      841 tgacggaatt tattgacaca atgcttgagt tattgatagc aggacaagaa agaatacttg
      901 atgagttaat accaaaaatg gatgcgactg aaaaattaac gctttattta actagtcatt
      961 ataaaaagat agattatgaa tttttgtatc tcctttcaat ggataaattg tttggaaata
     1021 aaagaaatag attaacatta attgatttag aaaaca
//
LOCUS       DATTQF010000258         1043 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.263, whole genome shotgun sequence.
ACCESSION   DATTQF010000258 DATTQF010000000
VERSION     DATTQF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.263"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            381..609
                     /locus_tag="VB241_002721"
                     /pseudo
     CDS             381..609
                     /locus_tag="VB241_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            710..946
                     /locus_tag="VB241_002722"
     CDS             710..946
                     /locus_tag="VB241_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245927.1"
                     /translation="MIRFDVNGSDHANSPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIIEPRLL"
BASE COUNT          419 a          150 c          143 g          331 t
ORIGIN      
        1 tttaaggtta atattaatga aattaaagat aaagttttat cagaacacaa tagcacagtt
       61 caattcattg atgaaccaaa agctatacaa tacagagaaa ctttaagatt aacttcactc
      121 taaaaatttg ggtagcccgc ctacccttat tatttttttc caacttttat gagtatgtca
      181 tatatcaata aaaaaacaac caaattgtaa aaggttttat ggttttggat atgaatgtaa
      241 ttattgtaaa aagtaattat ttattaatca atacattgta atattaagtg caacccaata
      301 aaaattcata aaaataccca caatgggcac tttattgtaa ggtgtttaca accacgaaaa
      361 cacatttaag tattgccaat atgagcgata actatcttat actaactgag cacgtataga
      421 gaaaattatt ctttaagacc tattgcacgt aaattagacc gatccgcatc tactatctta
      481 cgtgaaatta gcagaaataa tgtaaataag ttatatcaat ctgaaacggc tcaaaagaat
      541 tatgtaacta caagaaaact ttgtggtcat cctactggat ttactccaga acttagcaat
      601 atcattaagt aaaatttaaa aataccacta ctcttctgaa ttaatcgtga ttcgattttt
      661 taaaaccgat tttcttttat aaccaaggat ttatgtttta accactgata tgattaggtt
      721 cgatgttaat ggtagcgatc atgcaaattc accaaataat gaaagaatac ctacgccaca
      781 tatacacata tatactgaag aatataataa tggtggtatt gcgatcccat taaaagatat
      841 tgaagattta gaattaacag atgaaattat agaatcatta gacttcttta tgaagtatac
      901 taatataaaa cacgataatg ttataataga accaagatta ctgtaaaaga aatggcggga
      961 tacttattga agactatcga agaaagaatg aacgaatatt tcaactggtt aaaacaaaat
     1021 tatatcttca aagaattaga tag
//
LOCUS       DATTQF010000259         1013 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.265, whole genome shotgun sequence.
ACCESSION   DATTQF010000259 DATTQF010000000
VERSION     DATTQF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.265"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(86..760)
                     /locus_tag="VB241_002723"
     CDS             complement(86..760)
                     /locus_tag="VB241_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISSau6 family transposase"
                     /protein_id="HEP1245928.1"
                     /translation="MNYFRYKQFDKDVITVAVGYYLRYALSYRDISEILRERGIYVHH
                     STIYRWVQEYAPILYQIWKKKNKQAYYKWHIDETYIKIKGQWNYLYRAIDADGHTLDI
                     SLRKKRDNHSAYTFIKRLIKQFGKPQMIITDQAPSTKVAMSKVIKDFKLTPNCHCTSK
                     YLNNLIEQDHRHIKVRKISYQSINTAKNTIKGIECIYGLYKKNRRSLQIYGFSPCRVI
                     SIMLAS"
     gene            855..>1013
                     /locus_tag="VB241_002724"
     CDS             855..>1013
                     /locus_tag="VB241_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1245929.1"
                     /translation="MDKQVRNTTEIVRLAKQKSQKTREKVDKAISKFSIEGKAINFNS
                     IAKEANVSK"
BASE COUNT          332 a          161 c          166 g          354 t
ORIGIN      
        1 attacggtat gcatatcttt taaaaggttc tgttgcaaag ttaaaaaata agtgtctaac
       61 cttatgttta aaataatact tcgtattaac tagctagcat gatgctaatt acacggcatg
      121 gcgaaaaccc gtagatctga agagatctgc ggttcttttt atatagtcca taaatacatt
      181 caatgccttt gatcgtattc tttgccgtat tgatactttg ataacttatt ttccttactt
      241 tgatatgacg atgatcttgt tcaatgagat tatttaaata tttagaggta caatgacagt
      301 tgggggtaag tttaaaatct ttaatcactt tggacattgc caccttcgtt gaaggggctt
      361 ggtctgtaat tatcatttga ggtttgccaa attgtttaat aagacgttta ataaacgtat
      421 atgctgagtg attatctcgt ttcttacgta acgaaatatc tagtgtatgg ccatctgcat
      481 caatagcgcg atataaataa ttccattgcc ctttaatttt gatatacgtc tcatctatat
      541 gccatttata ataagcttgc ttattctttt tcttccaaat ttgatacaaa ataggagcat
      601 attcctgtac ccatcgataa attgttgaat gatgaacata gatgcctcgt tctctcaata
      661 tttcagatat atcacgataa cttaatgcat atcttagata gtagccaacg gctacagtaa
      721 tgacatcttt gtcaaattgc ttatatctga aataattcat cctctgcacc tcatttttgt
      781 tgactattat actatatttc taactttgca acagaaccga acaaatcatt ggaagtttga
      841 cggggtaatt atcaatggat aaacaagtta gaaatacaac agaaattgta cgtttggcga
      901 agcagaaatc acaaaagaca agggaaaaag tagacaaagc gatttctaaa ttttcgattg
      961 aaggtaaagc tattaatttt aattcaatag caaaggaagc taatgtttct aaa
//
LOCUS       DATTQF010000260          983 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.266, whole genome shotgun sequence.
ACCESSION   DATTQF010000260 DATTQF010000000
VERSION     DATTQF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.266"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..302
                     /locus_tag="VB241_002725"
     CDS             <1..302
                     /locus_tag="VB241_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEP1245930.1"
                     /translation="ILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFG
                     GLSIMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            376..537
                     /locus_tag="VB241_002726"
                     /pseudo
     CDS             376..537
                     /locus_tag="VB241_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            538..983
                     /locus_tag="VB241_002727"
                     /pseudo
     CDS             538..983
                     /locus_tag="VB241_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          359 a          118 c          152 g          354 t
ORIGIN      
        1 taatactttt tttatttctg aatcgtaagc taagattgga aatttataat aataactcta
       61 gtaaagggaa aataatttta tttccttcat taaaaaactt ttgctttaca atattttatt
      121 attttttatt tggcggtctt tcaataatgg ctctaagtat gttattaact ttaaatcctc
      181 aaaatataat aggctttatt ggttggttgg taatgactgc aggtttcttt ctgttaaaca
      241 tgtcatcgat tattgacaaa aaaatttatg tattatctaa aactaacacg gtggaaaaat
      301 gatggtttag ctggatttac tgcgagttct gtttcgtcaa tacttgtata ttggaccaat
      361 caaagaaatg aatttggaat aaaaggtaaa aacgattgga taggacataa actagacgat
      421 ggtatcgata gtgttaaaaa atctacagaa aaagccgtag atagtgttgg gaatgttgtt
      481 ggtgaaggtt caaaaagtat ttctaatcat ataagcccta agaaatggag ctggtaaatg
      541 ttgctatgcg aatctaaaat catcaataaa aaccccaaat atagaattat taaatataat
      601 gatgaatact taatggtcga tataataagc acttggatta gtttattttt tccatttatc
      661 aattggttca tcccaaaaag atatgtcaaa atcagtaaaa aagaatttga cgatttaaat
      721 attgttaaac ctgctaaaaa atgttttttg gccagttgca ggtagctctg ctttgctggg
      781 agttgcatta agaaaatata cacatttact tgacgttcaa cttgataaaa aattagttat
      841 tgccatatgt tgcatcacat ttatagggat tttaatattt tatgtacgcc taattaaaaa
      901 atcatcttta aatatttata atactaaaaa taaaaggtca aaaatttttt taatacctac
      961 actaaaaaat gtttgtttca cat
//
LOCUS       DATTQF010000261          980 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.267, whole genome shotgun sequence.
ACCESSION   DATTQF010000261 DATTQF010000000
VERSION     DATTQF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.267"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>980
                     /gene="mcrC"
                     /locus_tag="VB241_002728"
     CDS             <1..>980
                     /gene="mcrC"
                     /locus_tag="VB241_002728"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HEP1245931.1"
                     /translation="KGYQKLATEQFENIFDLYSAILIKGISSQLNSGLHHEYIEQTDS
                     LKVIRGKVDVKNSIQGLGVLSQRINCIYDEFSLNTYMNKILKTTMKCLIKTDISRKNK
                     IKLRKLLVHFNNVDTLDYRNIQWYHSFDRNNQTYKMLISICYLIFQGVIQTESKGQND
                     LMVFVDEQQISRLYEKFILEYYKKEFPELVVTSSNIQWSLDNDDNVNMLPVMRSDIML
                     RYKDKCLIIDAKFYKNTLHNYYDTKKIHSTNLYQIFTYVKNQQLNLKKKAIQVSGMLL
                     YAKTDENIVLNDKFHMSGSQIIIKTLDLNCNFTIIKKQLNGIVNDIFLLK"
BASE COUNT          406 a          104 c          143 g          327 t
ORIGIN      
        1 aaaaaggcta tcagaagtta gcaacagaac aatttgaaaa tatatttgat ttatattcag
       61 ctattcttat aaaaggtata tcaagtcagt taaatagtgg acttcatcat gaatatatag
      121 aacagacaga ttctctaaaa gttattaggg gaaaagttga tgttaagaat tctatacaag
      181 gcttaggagt tttaagtcaa cgtattaatt gtatttatga tgaattttca ttaaatacat
      241 acatgaacaa gattttaaaa actacaatga aatgtttaat aaaaacagat atttctagaa
      301 agaataagat aaagctccga aagttattag ttcattttaa taatgttgat actttagatt
      361 atagaaatat tcaatggtat cattcgtttg atcgaaataa tcagacgtat aaaatgttaa
      421 tatctatatg ctatttaatt tttcaaggag taatacaaac tgaaagcaag ggacaaaatg
      481 atcttatggt atttgtagac gaacaacaaa tatctcggct atatgaaaaa tttattctag
      541 agtactataa aaaagaattt ccagagcttg ttgtaacatc atcaaatatt caatggtcat
      601 tagataatga tgataacgtg aatatgcttc ctgtaatgag aagtgatatt atgttgagat
      661 ataaagataa atgtctaatt atcgatgcaa aattttataa aaatacatta cacaattatt
      721 atgatactaa aaagatccat tcaactaacc tgtatcaaat atttacttac gtgaaaaacc
      781 aacaattaaa tttaaaaaag aaagcaatac aggtctcagg aatgttgtta tatgctaaaa
      841 cagacgaaaa tattgttttg aacgataaat ttcatatgag tggtagtcaa atcattatta
      901 agacattaga tttgaactgt aattttacta ttattaaaaa acagttgaat gggatagtta
      961 atgatatatt tttactaaaa
//
LOCUS       DATTQF010000262          954 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.268, whole genome shotgun sequence.
ACCESSION   DATTQF010000262 DATTQF010000000
VERSION     DATTQF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.268"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..777
                     /locus_tag="VB241_002729"
     CDS             <1..777
                     /locus_tag="VB241_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP1245932.1"
                     /translation="GNLESLNKKESIKETAKVVRAKILQDESNEALLFMLEKAGIVIY
                     EKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHELGHLVLHRHIQFDLLSP
                     EEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLAVLKEKWYVSIQAIAMRA
                     YYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKMNSLLKLYFDRNILTPQK
                     LLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
BASE COUNT          364 a          120 c          164 g          306 t
ORIGIN      
        1 ggcaatttag aatcactaaa taaaaaagaa agcataaaag aaactgctaa agttgtgagg
       61 gctaaaattc ttcaagacga aagcaatgaa gcactattat ttatgcttga aaaagcgggt
      121 atagttatct acgaaaagcg aattaatgat agtatagacg catatagttt ttggagtaaa
      181 gatcagacac cttttattat actgggtaca aataaggggg tagcagttag aaggaatttt
      241 gatcttgcac atgagctggg acatctcgtt ctacatcgtc atattcaatt cgatttatta
      301 tctcctgaag aatataagac aattgaacat gaggcagata ttttcgcctc tgaattttta
      361 ctacctgaag aagcgtttaa aaaagacttt gatcaaatga caaaaaaatc aaatcctgat
      421 tatttagctg tattgaaaga aaaatggtat gtttctatac aagcaattgc aatgagagcc
      481 tattatctag gtctaatgtc tagtactcag tacagatatt tttgggcatc attaaataaa
      541 aaaggctata aaagtaaaga gcctttagac gacgtgattg aaatgtcacg tcctgtcaaa
      601 atgaatagtt tattaaagtt gtattttgat agaaatatac tgacaccaca aaaattattg
      661 aattatttaa aagttgatga aacatttttg aatcatctag caggtattaa tttaaaactc
      721 tttaaggatt atgttaatga aaatagagaa tataatataa cgaatctata taaataagac
      781 accaatattt aaacctcgag ctgaaaagat tatcatttca gttcgaggtt tttatttgca
      841 acatctgttg ataggtatcg acaaggacct tttaatgttg tgttttgtgc gatataacaa
      901 gctttttagg ctattaaaga atattcagaa ttaaatatat aattatgaat aaat
//
LOCUS       DATTQF010000263          945 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.269, whole genome shotgun sequence.
ACCESSION   DATTQF010000263 DATTQF010000000
VERSION     DATTQF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.269"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
BASE COUNT          407 a          166 c          101 g          271 t
ORIGIN      
        1 aaaaacaata catagccatt ttgaactaac ttgctaatct tttattgata ttcaactatg
       61 ttataaaaaa agcaaattga ccaaaatgga agtttgaatc aaaaaacatt aaatgtaccc
      121 atgacttaaa ttttatcgcc attatttaag tttggacata cacttttgtc atcaaacgct
      181 gaaaattagt tcgattgaaa tcacttcttg ttataaaaat tcttagtagc cctgttacaa
      241 aatattcgct acaaattatt caaatcaaca acaataacca tcaactaaga aattttaaac
      301 aatctactaa aaaataaagc gtttaaaagt tctgtttaga aaacatcaag ataagtataa
      361 acatctagaa aagtgaacat ctaagaaaat ccatctagtt attgccgtaa caattagaat
      421 aatcatttga aaaagtgcat tagacaacga aattcaccat caagataatg aagcaacaag
      481 aaacagcaca catcaatgaa ttaacacctt agattcatca tgagaaaatg catggcacaa
      541 ctagatttac catcaagata attcttaagc agcgaagaat taacatctag aaatgcataa
      601 acatcttaga ttttacatca agagatgcga taactataaa caactagaaa cgtcaccaag
      661 atagtgcaac aacaaagaat cagcatcttg aaatcgcact aacatctaag aatcaacatc
      721 aagtattata ttaacatcat agataacttc atcaagacag gcatccacat ctaagaaact
      781 catctagaat agctaacacc taacatcaaa gaatattcat caagaacagt atctaacaga
      841 actttttgac tatatatgaa ttaattatgt tactagctaa atcatctatt attaactttc
      901 acactcaatt tctacatcta atatcaattt attaacaata gaatt
//
LOCUS       DATTQF010000264          914 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.270, whole genome shotgun sequence.
ACCESSION   DATTQF010000264 DATTQF010000000
VERSION     DATTQF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.270"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            135..914
                     /locus_tag="VB241_002730"
                     /pseudo
     CDS             135..914
                     /locus_tag="VB241_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          348 a          106 c          140 g          320 t
ORIGIN      
        1 aaaattaccc attatatagt tttatttatt tttgatttgg tattcgtatt gtgttaaatt
       61 tacgtttaat taaataagtt aaatattata gggaatgata aaagtggtac ttaaatatag
      121 aaagaggtaa agttatggca aaatcatgct tgcatatact tactaataat gaatatgcga
      181 caacgcgttg ccaagatggc atagtcttat tttggccaat tgacggggaa atcgaactac
      241 aaaaatttcg taaaagtaaa ataattgaag atgatatata tatattatta atcatctgga
      301 tgtatttagt attaagaata ataaaaaaac gatcatgttg tatttgagta gcgattggtt
      361 tgcggaatta ggctttactt tctttaatta ccactataca gcaaagttaa ttaaatcatc
      421 ttataatttg aaatgtttac tattaaaatt gacttatcga taccttgata atcaacctct
      481 caatgacgct gatgttagaa aattacagga tattattaaa attattgcaa aagaagcaag
      541 tatggataaa aagattgcgc aaaatcaata tcgatatgcc tattatggtg atttgcgtga
      601 cgagctcgaa tatatttatc aaaatgtaaa tcaacgattg acattaaaaa gtgtcgctga
      661 taaattattt gtctcaaagt caaatttgtc atcacaattc cacttactta tgggcatggg
      721 ttttaaaaaa tatattgata ctttgaaaat tggtaaatcg attgaaattc tacttactac
      781 tgatagtact ataagcaaca taagtgaaca tttaggtttt agtagtagct caacttactc
      841 taaaatgttt aaaagttata tggatataac accgaatgaa tatcgtaatt tatcaaaatt
      901 taataaatct ataa
//
LOCUS       DATTQF010000265          914 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.271, whole genome shotgun sequence.
ACCESSION   DATTQF010000265 DATTQF010000000
VERSION     DATTQF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.271"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..611
                     /locus_tag="VB241_002731"
     CDS             <1..611
                     /locus_tag="VB241_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP1245933.1"
                     /translation="EDVKPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNGWRQRL
                     MVTPFTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVEMSAVQWLESGIFLWLGAS
                     LLITFGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHL
                     RKLGVDIASNHHINLISFVIILLYALGSIIAVYCISHFKRAE"
     gene            615..>914
                     /locus_tag="VB241_002732"
     CDS             615..>914
                     /locus_tag="VB241_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP1245934.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPTWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAIPFTF"
BASE COUNT          276 a          108 c          147 g          383 t
ORIGIN      
        1 cggaagatgt taaacctaaa ttttataaag agtatatgta tagtatgacg gtttatagtt
       61 tgttaagttt tagcttacta acttttccat tagatattat taatgaaaaa caaaatggat
      121 ggcgccaaag attaatggta acaccattta cttttactag ttattatatt tcaaaagtag
      181 tgaaaactat gctgcaattt gcaatagcga tattagtgat ttttatggtt gggcattttt
      241 ataaaggtgt tgaaatgagt gcagttcaat ggttagagtc aggaatattt ttatggttag
      301 gtgcgtctct attaataact tttggcatat tattttcttt gttaaatgat attcaaaaaa
      361 caagtgcttt agccaatatc gtaacaattg gattagcagt attaggtgga ttgtggtttc
      421 cgataaacac atttccgaat tggcttcaac atgttgctca tgttttaccg agctatcatt
      481 tgcgtaaact aggtgtagat attgcttcaa atcatcatat caatttaata tcatttgtta
      541 taatactctt gtatgcttta gggagtatta tagcagtata ttgtattagt cattttaaaa
      601 gggcggaata aaatatgaaa tttttaaaag atacttcaat tgctgaaata tcgtctatac
      661 tttatctaat ttttcctatt gccggtatat tttttaatga agtatatggt cccacatggt
      721 tgtatattat atcagtcatt gtcttttcgt tatcgtatct tatattagtt atagtaaata
      781 atagacttaa tacattaatg ttttacattt tgttgattat tcattatttt attatttgtt
      841 attttgtttt cagtgtacat ccaatgctaa gtttgttttt cttttatagt gcttttgcca
      901 ttccatttac tttt
//
LOCUS       DATTQF010000266          913 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.272, whole genome shotgun sequence.
ACCESSION   DATTQF010000266 DATTQF010000000
VERSION     DATTQF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.272"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(160..912)
                     /gene="sarS"
                     /locus_tag="VB241_002733"
     CDS             complement(160..912)
                     /gene="sarS"
                     /locus_tag="VB241_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HEP1245935.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMNDAQQDHAEQLLAQVNQLLADKNHLHLVFE"
BASE COUNT          300 a          113 c          139 g          361 t
ORIGIN      
        1 aagaataaaa tggaattttg aatgtgctga ggtttaattt cggaaatggt tttaagagat
       61 atagaaaata gaaagttgtt cataatgtgc tgaggtttta ttaaaaaata gaaaacacaa
      121 gtgcacttta gaatacagca cacttgcgta atagagatat tattcaaaaa caagatgtaa
      181 atgattttta tctgccaata attgattcac ttgagctaat aattgttcag catggtcttg
      241 ctgcgcgtca ttcatatgaa ttaaaatttt tctttcatct tcagttgagc gttcttttat
      301 tagatagcct tgctttttta aattattgag agctctaaca gtttgagggt atttatggtg
      361 gattgtttca attaaatctt taagaagaac gatgttttta ttttgagaag tgataatagc
      421 tagaattgtg aattctacaa aacttaatgt tagatgcttt ttgataatat tcttgaaata
      481 cattgtatac atcatcaagt ttagaaattc tttactatct tttggtatca tctgtgattc
      541 actttgatct gcaagattaa attgtttaat gatttgatca aacaatgtaa cacgttctgc
      601 aattttctct cgttgttctt cagatattga aatgtaagta ttacgctcat caattttact
      661 tcgaacttta ctaatatatg aatgtttcac aagtactttt atatgctgta ctaaatccga
      721 ttgtttataa cataaatctg aaacaatctt cttaaatgga agtgtgtttt cttgctgatg
      781 aaataaataa gtcagtaata taaattcttt tatagtcata tcgacttcag gcttgacttt
      841 tttcttaaaa cgaaacatat atgcttcaat gattataaaa tctctaattt tgtcatggtt
      901 attatatttc att
//
LOCUS       DATTQF010000267          906 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.273, whole genome shotgun sequence.
ACCESSION   DATTQF010000267 DATTQF010000000
VERSION     DATTQF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.273"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            6..782
                     /locus_tag="VB241_002734"
     CDS             6..782
                     /locus_tag="VB241_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016930909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP1245936.1"
                     /translation="MTATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKYNTSVESI
                     KQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNIIANRYGVS
                     VDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNLYTAGQCT
                     WYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGPYGHVAYV
                     ERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          297 a          179 c          153 g          277 t
ORIGIN      
        1 ttgcaataac agcaacatct ggtgcagctg catttttaac gcatcatgat gcgcaagctt
       61 ctacacaaca tacagtacaa tctggtgaat cattatggag tattgctcaa aaatacaaca
      121 cttcagtaga gagtattaaa caaaataacc aattagataa caacttggta ttccctggtc
      181 aagttatctc agtaggtgga agtgatgcac aaaatacgtc aaacacttct ccacaagctg
      241 gttcagcatc atctcatact gtacaagctg gtgaatcatt aaatatcatc gctaacagat
      301 atggtgtttc agttgatcaa ttaatggcag ccaataactt acgtggttat ttaattatgc
      361 ctaaccaaac attacaaatt cctaatggtg gatcaggtgg tacaacacca acagctacaa
      421 caggtagcaa tggcaatgca tcatctttta atcaccaaaa tttatacact gctggtcaat
      481 gtacatggta cgtatttgac cgtcgtgctc aagctggtag tccaattagc acatattggt
      541 cagacgctaa gtattgggct ggtaacgcag ctaatgatgg ttaccaagta aacaacacac
      601 catcagttgg ttcaatcatg caaagcacac ctggtccata tggtcatgtt gcttatgttg
      661 aacgtgtcaa tggtgatggt agtatcttga tttctgaaat gaattacaca tatggtccat
      721 acaatatgaa ctaccgtaca attccagctt cagaagtttc tagctatgca ttcatccatt
      781 aattaaataa attgtactga tatatactag caattcacat catgtgagat tgctagtttt
      841 ttatttttga aaaaaatttt cattttggta caaaaaatta tctcaccctt ccctatcata
      901 cttatt
//
LOCUS       DATTQF010000268          868 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.274, whole genome shotgun sequence.
ACCESSION   DATTQF010000268 DATTQF010000000
VERSION     DATTQF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.274"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..491
                     /locus_tag="VB241_002735"
     CDS             <1..491
                     /locus_tag="VB241_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="base excision DNA repair protein"
                     /protein_id="HEP1245937.1"
                     /translation="IRTILAQQVSIEQANKLFLLFINTYGEIFELNNKEYTFLNWDKI
                     FSRIYKEQKVLVGTTNNRKQAIITLAELHIKNGLRENVLQVISSIKGIGSWTVQMFML
                     FQFPHVAFENIPVADIGLHRAIEKNHDLPHKSIDKSKGESYFQNMKSIDVFKYWYIYM
                     VE"
BASE COUNT          326 a          105 c          129 g          308 t
ORIGIN      
        1 taatacggac tatacttgct cagcaagtga gcatagaaca agccaataaa ctatttttat
       61 tatttattaa tacttatggt gaaatttttg aactaaataa taaagaatac acttttttaa
      121 attgggataa aatattcagt cgaatatata aagaacaaaa agttttagta ggtacgacta
      181 ataatagaaa gcaggctatc ataactcttg ctgaattaca tataaaaaac ggcttaagag
      241 aaaatgtttt acaagttatt tcaagtataa aaggtatagg ttcttggaca gtgcaaatgt
      301 ttatgctatt tcaatttcct cacgtagctt ttgaaaatat tcctgtagca gacataggat
      361 tgcacagggc catagaaaaa aatcatgatc ttccacataa atcgattgat aaaagtaagg
      421 gagaatctta cttccaaaac atgaaatcta tagatgtgtt taaatattgg tatatataca
      481 tggttgaata gatgtttaag cagtttgttg tacagtaaaa ataaaaccta ttaaatataa
      541 ggattgctta atctcttttc taattattca actcccgatg taatattctg taaattactt
      601 tacagaatat gtttacttta cgtacttttt aaatgaaagg taagagtaga taaacttttt
      661 aaagacctat ttctgaaata atagaagata ctactttatt tatataaatg ttggattatc
      721 ttaaataggc agtatagtga ctatttatca tgccagttct atcatttttt taaaggaatt
      781 attaagatga ttatcacatt actaccggaa aggtttaagg taattgttgt agaaaattct
      841 caatatattt tttcagcgat acattttt
//
LOCUS       DATTQF010000269          854 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.275, whole genome shotgun sequence.
ACCESSION   DATTQF010000269 DATTQF010000000
VERSION     DATTQF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.275"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>854
                     /locus_tag="VB241_002736"
     CDS             <1..>854
                     /locus_tag="VB241_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEP1245938.1"
                     /translation="MIKGKQHFKTIRFINTVCKSVLLVLIASVMIENTSVIKDLNKIK
                     ETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVD
                     NDNFSPDEGNVILVNNQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAY
                     HSWFEFVQNKNNKESKLSIQFINKNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSK
                     DFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLN
                     FSQIIIAI"
BASE COUNT          335 a          110 c          144 g          265 t
ORIGIN      
        1 atatgattaa aggtaagcag cactttaaaa caattcgttt tattaataca gtttgtaaaa
       61 gtgttctctt agtactaata gctagtgtaa tgatagaaaa tactagtgtt attaaagatt
      121 tgaataaaat aaaagaaact gagaaatatt ggaatgtatt agatgattat tacacgattg
      181 aatttgcacc ttatcacgaa acaaaacaaa gtttgattga taatatggtg cgatcagaac
      241 aattagtaaa ggctagtgaa gcagaaaata atgcgatttt attcaaacca aagggtgact
      301 ccgttgacaa tgacaacttt tcacctgatg aggggaatgt aatattagta aataatcagt
      361 tttggtcgat ttatcacaag cagtttcaac ctgatattcc aataaaaaat caaaaaaata
      421 atgtcgaagt aattattcca caaaagtttc atgcaatgcg taatgaaatc aatcaagcat
      481 atcattcatg gtttgaattt gtacaaaata aaaataataa agagagtaag ttatctatac
      541 agtttatcaa caaaaatgat tatcgaattt ttacatttga tgcacgagat agtcgccatt
      601 tgtcatttat agaggcgcca atcattgtga atgttcaggc atcagattta tcgaaggatt
      661 tttattatgc catgatcagt caaggcgggt atttattcaa aaattatgac gcgctagtaa
      721 aaaatattga aaagtatcat cttgatgggg aaatcagtgg aataaccaat tataaagata
      781 gcgtgatgga aatgtatcat gaaaacaatt tgaaattaac agtactcaac ttttcacaaa
      841 tcattatcgc aatc
//
LOCUS       DATTQF010000270          842 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.276, whole genome shotgun sequence.
ACCESSION   DATTQF010000270 DATTQF010000000
VERSION     DATTQF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.276"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            311..727
                     /locus_tag="VB241_002737"
     CDS             311..727
                     /locus_tag="VB241_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245939.1"
                     /translation="MFKVNYSILSYYPDIYLHSNLAVGVAFEIIGESYHKNEIKFITQ
                     RRKILSFDDEIDNLELINMFLDGLKCEFEQTNQSLKSYKKKFVNNFYFENIEYKMFDS
                     LKEVNNFIENTYKYVLHHGLDKKERLKKTEKELLNS"
BASE COUNT          330 a           88 c          131 g          293 t
ORIGIN      
        1 ataaaaaaga ttggaatgga tcgtcttgaa atgctccctt caaagttttc attttttcaa
       61 tgtctacttc gaagggggca tttccattaa atttttatag ctttttatat ttatataatg
      121 agcatataag tttaagtaag tgcgagtgaa gggaataaaa aagctcaaat gtaccaaatt
      181 gttaatctta ataaatctct actttataaa gattgaatgg acattcgagc gttaatcagt
      241 caggagggac tttccctcct acaatttaat aataatactt gcttcaccac tgtacaagga
      301 gtgattagtt atgttcaaag tgaattattc gattttaagt tattatccag atatttatct
      361 ccatagtaat ttagctgtag gtgtggcatt tgaaattata ggagaaagtt atcataagaa
      421 tgaaattaaa tttataaccc aaagaagaaa aattttatca tttgatgatg aaatagacaa
      481 tttagaactt attaatatgt ttttggatgg gcttaaatgt gaattcgaac aaactaatca
      541 atcattaaaa agttataaga aaaagtttgt taataatttc tactttgaga atattgaata
      601 taaaatgttt gactcattaa aagaagttaa taattttatt gaaaatacat ataaatatgt
      661 attacatcat ggtttagata aaaaagaaag acttaagaaa actgagaaag aacttttgaa
      721 tagctaagta aaaaggtcaa acatattgtt ctgaatataa ataagtaata accgtacctt
      781 tattgatgcg gttatttttt atgtaaaaaa acgaaaaaag tttttaaaaa agtgttggat
      841 ac
//
LOCUS       DATTQF010000271          819 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.277, whole genome shotgun sequence.
ACCESSION   DATTQF010000271 DATTQF010000000
VERSION     DATTQF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.277"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..475)
                     /locus_tag="VB241_002738"
     CDS             complement(<1..475)
                     /locus_tag="VB241_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP1245940.1"
                     /translation="MSQNTNHSYYHQNQHAQAISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFY"
BASE COUNT          334 a          122 c           95 g          268 t
ORIGIN      
        1 tataaaaaat aggattttga agaaaaatac caattagact cgcaataatt aatgttatta
       61 atgtaacgaa taaatacttg cctatactcg atgcatctcc aacgaagaaa taaccaataa
      121 taccaaatgc tataaatgca aaaattgcta atgcgatatt tttatagaaa atatctggtc
      181 ctaaattttg taaatacgtg gtaaacgttg cgtatgacaa taagccaatc actacagcgt
      241 atatatgtga aataattaaa ccgaaccgtc tcgctctttc aaaaacaagt gttgctaaaa
      301 taataatcag taatccaaat gacaagggtt gtcgccaact taatggtaaa aactgaccta
      361 gatagcatcc tatgccaaat ataatccaat aatacataaa ataaagccac actttactta
      421 ttgcttgagc atgctggttt tgatgataat atgaatgatt tgtattttgc gacaaaataa
      481 ttcctccttc tactgtcgtt ttatgcgtca aattttacag taaattttgc ttcatcaaaa
      541 gaaataacct ttaacaagta tagtaatttt acattttata atgttacaaa atataatttt
      601 ttataattag ttaaaatcac taaaacgctt ttatacacta tcaaatcagc atttataaaa
      661 atatgaaccg atatcctaaa atgttaataa tattacaaga taataacaaa ccacacaaag
      721 ctacttattt ttgataatat ggaaagcgta atataaaacg aaaacttaat ttactatata
      781 aattgtctta ataattttta aaagtagtaa atcttaatt
//
LOCUS       DATTQF010000272          811 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.278, whole genome shotgun sequence.
ACCESSION   DATTQF010000272 DATTQF010000000
VERSION     DATTQF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.278"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>811
                     /locus_tag="VB241_002739"
     rRNA            <1..>811
                     /locus_tag="VB241_002739"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          194 a          178 c          253 g          186 t
ORIGIN      
        1 cgtggagctt tactgtagcc tgatattgaa attcggcaca gcttgtacag gataggtagg
       61 agcctttgaa acgtgagcgc tagcttacgt ggaggcgctg gtgggatact accctagctg
      121 tgttggcttt ctaacccgca ccacttatcg tggtgggaga cagtgtcagg cgggcagttt
      181 gactggggcg gtcgcctcct aaaaggtaac ggaggcgctc aaaggttccc tcagaatggt
      241 tggaaatcat tcatagagtg taaaggcata agggagcttg actgcgagac ctacaagtcg
      301 agcagggtcg aaagacggac ttagtgatcc ggtggttccg catggaaggg ccatcgctca
      361 acggataaaa gctaccccgg ggataacagg cttatctccc ccaagagttc acatcgacgg
      421 ggaggtttgg cacctcgatg tcggctcatc gcatcctggg gctgtagtcg gtcccaaggg
      481 ttgggctgtt cgcccattaa agcggtacgc gagctgggtt cagaacgtcg tgagacagtt
      541 cggtccctat ccgtcgtggg cgtaggaaat ttgagaggag ctgtccttag tacgagagga
      601 ccgggatgga catacctctg gtgtaccagt tgtcgtgcca acggcatagc tgggtagcta
      661 tgtgtggacg ggataagtgc tgaaagcatc taagcatgaa gcccccctca agatgagatt
      721 tcccaacttc ggttataaga tccctcaaag atgatgaggt taataggttc gaggtggaag
      781 catggtgaca tgtggagctg acgaatacta a
//
LOCUS       DATTQF010000273          808 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.279, whole genome shotgun sequence.
ACCESSION   DATTQF010000273 DATTQF010000000
VERSION     DATTQF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.279"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            154..531
                     /locus_tag="VB241_002740"
     CDS             154..531
                     /locus_tag="VB241_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1245941.1"
                     /translation="MSYSIIRVVKVKSKTNTRGIQRENKNYENIDIDLSKSYLNYDLV
                     NDTKFDFNKKIDEKIEKNYKGKRKIRTDAIKHIDGLITSDNVFLINYLKKRQNSFLSM
                     LKRSWKKNTVKKIFYMRQYIWMR"
     gene            471..797
                     /locus_tag="VB241_002741"
     CDS             471..797
                     /locus_tag="VB241_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEP1245942.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVIPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSGYKQKTEYHKQMYMREKQ
                     IEDHLK"
BASE COUNT          338 a           90 c          137 g          243 t
ORIGIN      
        1 aacttaatgt cccaagctct ttttgtgatg acaaaaaaca agcctcgcag agcacacgtt
       61 tttaatgact tatttggttt ttaataagtc tagtatgtta aaacttttct gtgtttaggt
      121 gttataatca tatgcagtgg aagttgattt attatgtctt attcaattat tcgagttgtt
      181 aaagtgaaat caaaaacaaa tacaagaggt attcaaagag aaaataaaaa ttatgaaaat
      241 atagatattg atttaagtaa gtcatattta aactatgatt tagtcaatga tactaagttt
      301 gattttaata aaaaaattga tgagaaaatc gaaaaaaatt ataaagggaa acgcaaaatt
      361 agaacagatg ccattaaaca tattgatgga ttaattacat cagataatgt gtttttaatc
      421 aattatctga agaagagaca aaatagtttt ttaagcatgc taaagcgttc ttggaagaag
      481 aatacggtaa agaaaatctt ttatatgcga cagtacatat ggatgagata acaccacata
      541 tgcattatgg cgtaattcct attactaaag atggacgttt aagtgcgaaa gaagttgtag
      601 gtaataaaaa agcattaacc gaattccaag atagattcaa tacgtatatt aataagcaag
      661 gctatgattt aaaacgagga atttctagac aacttacaaa agaaaaacat gatcaagtta
      721 gcggctataa acaaaaaaca gaatatcata aacaaatgta tatgcgcgag aagcaaatag
      781 aggatcattt aaagtgagaa aatgataa
//
LOCUS       DATTQF010000274          787 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.281, whole genome shotgun sequence.
ACCESSION   DATTQF010000274 DATTQF010000000
VERSION     DATTQF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.281"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..245
                     /locus_tag="VB241_002742"
     CDS             <1..245
                     /locus_tag="VB241_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245943.1"
                     /translation="SGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGYLY
                     TIGTIQYLILFIALLIFIYLIIIFYFTRREQHETKN"
     gene            229..744
                     /locus_tag="VB241_002743"
     CDS             229..744
                     /locus_tag="VB241_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEP1245944.1"
                     /translation="MKLKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVTELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          294 a          101 c          125 g          267 t
ORIGIN      
        1 cttcagggtt aactcgtcca caaactataa ataaactttc tagcagtagt aacttaagag
       61 tgatgcttaa ttatgcagaa acgttatatt ttatttttaa tatcgcattt ttacttatgg
      121 gtggttactt atatacaata ggaactattc aatacttaat attatttatt gcattattaa
      181 tttttatata tttaataata atattttatt ttacaaggag agagcaacat gaaactaaaa
      241 actgaattta aagggaacaa tataccatat gaatacgcag caggtgcaga tgtgagtgat
      301 tctattaacg ggaatccaat taaatcattt ccatttgaag taactgaatt accggaaggg
      361 actaaatatc ttgcttggtc tttaattgac tatgatgcaa tcccagtatg tggctttgct
      421 tggattcatt ggagtgtagc taatgtaagt gttagtggca attcaatttc tattaaagca
      481 gatttatcaa gaacaaaggg cgactatgta caaggtaaaa atagcttcac tagtggtttg
      541 ttagctgaag atttttcaga aatagaaaat cactatgtag gaccaacacc acctgataaa
      601 gatcatcaat atgaattaac agtttatgcg ttagatcatt ctttaaattt gaagaatggg
      661 ttctacttga atgaattttt aaaagaagta aatcaacata aaatagatca aacaagtatt
      721 aaccttatag gaagaaaaat ttaatattaa atatctcatc aatataaaat tgttcaatta
      781 aaagtac
//
LOCUS       DATTQF010000275          784 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.282, whole genome shotgun sequence.
ACCESSION   DATTQF010000275 DATTQF010000000
VERSION     DATTQF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.282"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..650)
                     /locus_tag="VB241_002744"
     CDS             complement(<1..650)
                     /locus_tag="VB241_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP1245945.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSI"
BASE COUNT          267 a           98 c          117 g          302 t
ORIGIN      
        1 ttgatagata gaaattgtgc tttttcataa atatttaaaa tattattttt aagtgatgac
       61 caatattctg gatggttatc acttattatt ttgtattttt caaataaaga tgcctcagtt
      121 agaagatgat tattctcttt attgggagca atcaatacaa ttttaccttc gcatactttt
      181 tcagaatgaa cttctcttag tttaggttga tttctgctaa tcccgatgtc atacgtatga
      241 ttattaatat ctttttcaat attttcattt ttgacatgga gagaaacatc gataaacgga
      301 tgctcgttaa agaaagattt taaaaatttg ggcataatga atgtcgcgat atatgaagac
      361 acgacaacat ttaatttcga ttgaaacatc gtctttttaa gttggatatg tttgatgcca
      421 ctttcatatt gttcaataaa actttgcgca attggaagaa atgtatgacc atcttcagtc
      481 aagataattt gatttttata agtttcaaat agtttcacat tgagatgctg ttctaaattt
      541 ttaatttgct tatgtataga aggtatagtg agattaattt cttcactagc taatcgatag
      601 tttaacgtct tcgctaaaat gacaaatgta tagtaccaat ccaaattcat gaacttacct
      661 ctttcaaaaa aagttaataa ttcattagat aatatttaat ttttattgca taaaaataat
      721 attaatatta tatcagaggt tatcgagggg gtgaagtaat atgattcgaa ttcaagataa
      781 taca
//
LOCUS       DATTQF010000276          780 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.283, whole genome shotgun sequence.
ACCESSION   DATTQF010000276 DATTQF010000000
VERSION     DATTQF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.283"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            185..>780
                     /locus_tag="VB241_002745"
     CDS             185..>780
                     /locus_tag="VB241_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HEP1245946.1"
                     /translation="MMIHSKRLKMCLCLIILSVFIGACGMKKEESSKDKQIKENFNKT
                     LSLYPTKNLEDFYDKEGFRDEEFDKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRN
                     TRTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKQIADDKLRKEIENFKFF
                     VQYGDFKDINDYKDGDISYNPNVPSYSAEYQLSNNDYNV"
BASE COUNT          321 a           82 c          144 g          233 t
ORIGIN      
        1 ttggttaggc caataaaaat tataaagata tttgcgcttt tttaaataat ttgtgatgtt
       61 aatatgtgaa aaatacaact aacaattaaa agccacgagg atttaatcat aaaatttcct
      121 ctagtattat taatattgga atttgaactt aactttgcat caaaaataaa aatggggatg
      181 tgaaatgatg attcattcaa aaaggttgaa aatgtgtcta tgcttgataa tactatctgt
      241 ttttataggg gcttgcggaa tgaaaaaaga agaaagtagt aaagataaac aaattaaaga
      301 aaacttcaac aaaacgttga gcctgtatcc aactaaaaat cttgaagact tttatgataa
      361 ggaaggattt cgtgacgaag agtttgataa aggagataaa ggcacttgga ttgttgattc
      421 tgagatggta gttgagctga aggataaaaa aatggaatct agaagtatgg tgctctatat
      481 aaatcgtaat actagaacaa cgaaaggtaa ttttattgtt agagaattgt gggaagatag
      541 taaagggtat gcacaaagta aagatacaaa atatccagtg aaaatggaac ataatcgaat
      601 tatcccaacc aaacagatag ctgatgataa attaagaaaa gaaatcgaaa actttaaatt
      661 ttttgtacaa tacggtgact ttaaagatat taatgattat aaagatggtg acatttcata
      721 taatccaaat gtacctagtt attcagcaga atatcaattg agtaacaatg actacaatgt
//
LOCUS       DATTQF010000277          760 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.284, whole genome shotgun sequence.
ACCESSION   DATTQF010000277 DATTQF010000000
VERSION     DATTQF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.284"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..229
                     /locus_tag="VB241_002746"
     CDS             <1..229
                     /locus_tag="VB241_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEP1245947.1"
                     /translation="DLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYK
                     LVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            213..>760
                     /locus_tag="VB241_002747"
     CDS             213..>760
                     /locus_tag="VB241_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEP1245948.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTANDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTII"
BASE COUNT          311 a           87 c          116 g          246 t
ORIGIN      
        1 tgatttagga ttgaaaaatt cacaagttgt tcagtttcca ataagtaata tttctagcaa
       61 gaaaatcttg cacaataaat ttaaatcaat agttaacact gaagaaattg atgaaaatat
      121 ttctttttat aaacttgtga acaagttttc acctgaagta gcacctgaag atacagtgga
      181 tttacttcag ataataaaaa ggaggagtaa agatggaaat tttaaatgat tctgtaaaaa
      241 gttttgaaaa actttattta caattacaag atggttttcc agtgattgtt cctactgata
      301 ctaattataa tttatgtagt ttaccaaaca acgatctttg tatcgataaa atatttgaat
      361 ataaaaagcg atcgaaagat aagccattat cattatttat tgataagcca gaggattgga
      421 aattgtatgg agataatcaa aatacggaaa tagttgataa actagttgaa atattttggc
      481 ctggaccatt aaatattatt ttaaaaaata aaacaagcta taattatatg ctcaataatt
      541 cagatagtat agctatagga tgtgtacaaa ataaaacgat gagaaggttt atttcatata
      601 ttaattcacc tatagcaatt acttcagcaa atatctctgg aactgccaat gatattttaa
      661 taactgaaaa tgaagcgatt aagcacatgg gtgaaaatgt aagatatatg ctaagaagtc
      721 aaaataaaac taactataaa acgtctagta cgattattaa
//
LOCUS       DATTQF010000278          723 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.286, whole genome shotgun sequence.
ACCESSION   DATTQF010000278 DATTQF010000000
VERSION     DATTQF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.286"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            240..518
                     /locus_tag="VB241_002748"
     CDS             240..518
                     /locus_tag="VB241_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245949.1"
                     /translation="MENNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKDAKENNDSNEVAYLLKGGKVTKVDGNQNSVSFVPG"
BASE COUNT          294 a           96 c          121 g          212 t
ORIGIN      
        1 acaattattt tattcatgcc atagacagaa tatacataaa tactagtaaa gggctatttc
       61 ctgaatctga actcgtagca tggggataaa gaaagtttac ttttaaaaat ttcatattta
      121 attttaaaaa ttttaacctt ccaatgtgaa ggttattttt attgtccaaa acgtgctgat
      181 gacattttaa aagcaagtat ggaatatcag tcgacagact ataaacggag gtatatctca
      241 tggaaaataa tgaaagtaat attactgatg taactcagaa cgaagagcaa ctagataaca
      301 gtgatgaaca atcacaacag aatgagaaaa cattttctca agaagaagta tcacaattga
      361 ttaaagagcg tatagctaga gaacgcaaaa aatcagatga acgtattaaa gatgccaaag
      421 aaaacaatga tagcaatgaa gtagcttatt tattaaaagg tggtaaagtt acaaaagtag
      481 atggcaatca aaatagtgta tcttttgtgc cagggtaaca agcaaccgaa ttgttatttg
      541 acagtaaacc gaattcaatt gttatgttac ataactatcc tggccaatca ggattttcag
      601 aatatgattt atttacattt tttaagcatc catcaataaa atcaatgaca atcgttacta
      661 ataaggaaca agtaaaattt attaccaagt cagataggtt tcaaggtaaa atagtgagta
      721 aat
//
LOCUS       DATTQF010000279          722 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.287, whole genome shotgun sequence.
ACCESSION   DATTQF010000279 DATTQF010000000
VERSION     DATTQF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.287"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
BASE COUNT          308 a          103 c           93 g          218 t
ORIGIN      
        1 ttatgaatca gaatattatc ttcttgaaaa aaatttaatt aagacacaat ttatcaaaag
       61 tactaaaagt agatgtctat atgaagaaac cacttatcac atattgacga atggagacat
      121 cttaaaccaa cccggaaatt tgaaattatg gacgtaggca aggcttttgt ccaaccgttt
      181 cattgcatga atttggaaaa acatgtcaga tacctttatg aaatttccga atatttgcat
      241 ttaagtaaat cataaatttc agaagtatta tgataatata taccattaaa accaattact
      301 aattcattgt tctaactatg caggtaactt tccgcaataa gtccaaaata aatctaatgg
      361 ataaaaaata atcaaaactt taactttaat caatattgaa ctgcaaaaat ttggcacaag
      421 ctaaaacaat gattaaaccg acagggattg ccaatgacac aggtaaatca gtagaagaaa
      481 tgatgtgtag aagttcacaa gtaaactgga aaatagcaat taattaaaat tcgtttcaac
      541 ctttatacat actaagcaaa attacacact ttataaaaat aatattaatt ttgtaaaatt
      601 cagcttattt ggaggattaa aagcaatgat aaaacaggtt ctaagaaaaa gtttacatac
      661 agtaaaagaa catatcgata aaagtaactt ccaaaaaaac ttaatatttt cacttgtcat
      721 at
//
LOCUS       DATTQF010000280          686 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.288, whole genome shotgun sequence.
ACCESSION   DATTQF010000280 DATTQF010000000
VERSION     DATTQF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.288"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            105..392
                     /locus_tag="VB241_002749"
     CDS             105..392
                     /locus_tag="VB241_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245950.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEI
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            398..>686
                     /locus_tag="VB241_002750"
     CDS             398..>686
                     /locus_tag="VB241_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEP1245951.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERL"
BASE COUNT          295 a           58 c          105 g          228 t
ORIGIN      
        1 caaataaacg aaattaaata ttttaattat tgtatatata gttgctctaa atggttgcgg
       61 agtataaaat atattttgtt aaatatttta ttaagaggtg cgctatggaa aaggggattt
      121 tcaattacga taatgcgaat gtattaaaat tagatactaa tcagttaaat gaaaacataa
      181 aagtcattga cgatatattt aaaaattatg agcagataga gccaactata gaaatcgaaa
      241 atggaaatac aaaattaaaa ttaaatggat attttattgc gtcaattata agcccattaa
      301 atttaaataa acttaataat ttatatgttg aagaagaatt ttatcataca tataacgaat
      361 taattgttaa atatactgag gtaaaggaat aattataatg aaaaaaatat ttatgatggt
      421 acatgagtta gatgtcaata aaggtggtat gacctcttcg atgttcaata gaagtaaaga
      481 gttttatgat gcggacatac ctgctgatat tgttactttc gattacaaag gaaactatga
      541 tgaaattatt aaagctttga aaaaacaagg taaaatggat cgaagaacga aaatgtataa
      601 tgtatttgag tattttaaac aaatttcaaa taataaacat tttaagtcta ataaattgtt
      661 atataaacat atttcagaaa gactaa
//
LOCUS       DATTQF010000281          679 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.289, whole genome shotgun sequence.
ACCESSION   DATTQF010000281 DATTQF010000000
VERSION     DATTQF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.289"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>679
                     /locus_tag="VB241_002751"
     CDS             <1..>679
                     /locus_tag="VB241_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEP1245952.1"
                     /translation="PDKNNYIPENGNVFFINNNFLNFYKNINHDFKMIVNHSDKINVF
                     IPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFDRTTNLKFGYLDSP
                     IVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEISGITNYKDSVLNE
                     LNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIHGYSLFSSNVRY
                     LTISILLS"
BASE COUNT          263 a           92 c           88 g          236 t
ORIGIN      
        1 cccgataaaa acaattacat tcctgaaaat ggcaatgtct tttttatcaa taacaatttt
       61 ctgaatttct ataaaaatat aaatcatgat tttaaaatga ttgtcaatca ttcggataaa
      121 ataaacgtgt ttattcctcc aaggttacaa tatcaaaaat ctgaaattca aaaaaatcac
      181 caaggatgga ttgactttca aaagcaacaa aacaaaaaag tagatgtgca agtactgtca
      241 aaaaacgtat ctgttttttc ttttgatcgc acaactaatt tgaaatttgg atatctagat
      301 tctccaatag taatgttgtt gacacctgac gatttaacag gagattttta tttagctgct
      361 gtttctcaag gtggatatct atttaaagat ttagatacac ttaaacagtt gcttaaagat
      421 aatcacctag aagaagaaat tagcggtata actaattaca aggattctgt gttaaatgaa
      481 ttaaatgaaa cgaaaacaaa aatgataata acaatagtta ctattattat taacgttttc
      541 attcttttga tagctactgt atttgaaaca attcaatatt ttagctggaa taaaaagcaa
      601 ctattattaa gaaaaattca tggttattca ttattcagca gtaatgtacg ttatttaaca
      661 atttctattt tactttcta
//
LOCUS       DATTQF010000282          670 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.290, whole genome shotgun sequence.
ACCESSION   DATTQF010000282 DATTQF010000000
VERSION     DATTQF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.290"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..128)
                     /locus_tag="VB241_002752"
     CDS             complement(<1..128)
                     /locus_tag="VB241_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HEP1245953.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKIDNFDGYNSIE"
     gene            complement(135..467)
                     /locus_tag="VB241_002753"
                     /pseudo
     CDS             complement(135..467)
                     /locus_tag="VB241_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(538..663)
                     /locus_tag="VB241_002754"
     CDS             complement(538..663)
                     /locus_tag="VB241_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245954.1"
                     /translation="MIDSQLDGNVTAKELSESFKELVEEFERIEKANKNSNSKDK"
BASE COUNT          202 a          118 c           79 g          271 t
ORIGIN      
        1 atttctatcg aattataacc atcaaaatta tcaattttat catcaaagtc tatcactttt
       61 ccaattaata ttttattatt agtaagcgtt aatttgacta atttgcctat gtatgactgt
      121 aaattcattt ttaatcactc ctttttaata tacggaacta catgaaaacc agttttagaa
      181 taatgaactt cacctaattt catttcagta ttgttctcta cccgatattt accttcacgc
      241 tctttaaaga acttgtctcg ccaattgccg agatgtggca ctgtggtact tctacgccaa
      301 ggatgcatag gtgacgcatt tacacctggt atcatacctt taactttata tatttttccg
      361 ttgagtgaat gacataattt agatgtttta ctatctattt tggcaacata tttatatttg
      421 ccatcttcac caagttcttt taaatatgtt aacttttgtg attctgatat ttcagtaaat
      481 agttaaaaaa gtgtataaaa acagcaccgc tttctcttta tctgtgtaga aaggacgcta
      541 tttatctttt gaatttgaat tcttattcgc tttttctatg cgttcaaatt cttcaactaa
      601 ttctttaaaa gattcactca attcttttgc agttacattt ccatctaatt gtgaatcaat
      661 cataattaaa
//
LOCUS       DATTQF010000283          668 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.291, whole genome shotgun sequence.
ACCESSION   DATTQF010000283 DATTQF010000000
VERSION     DATTQF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.291"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(77..439)
                     /locus_tag="VB241_002755"
                     /pseudo
     CDS             complement(77..439)
                     /locus_tag="VB241_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          196 a          112 c           74 g          286 t
ORIGIN      
        1 agagtattgt atagctagga aacctctcat cattctgcaa tgcataaatt ttatttttta
       61 aatataattt atgacctaaa gaatgtctat tctgtttttc aggatttatt tctattttta
      121 ttttaccact ttcaatcatt tctttcatct ggtttttagc tttttcctgt aattttgctt
      181 cttttacttc tacttgatat ttaccttcac gctctttaaa gaacttgtct cgccaattgc
      241 caacatgtgg cactgtggta cttctacacc aaggatgcat aggtggcgca ttcacacctg
      301 gtatcatatc tttaacttta aatatttttc cgttgagtga atgacataat ttagatgttt
      361 cactatctat tttggcaaca tatttatatt cgccatcttc accaagttct tttaaatatg
      421 ttaacttttg tgattctgct atttcagtaa ttagttaaaa aaacacataa aaatagcacc
      481 actttctctt tatctgtgta gaaaggatgc tttaatacca tgcatatata tattcattcg
      541 gaaatttatt ttcatcaata aaatttaata ttgcatgaaa tgatttatct ctataaatta
      601 aaataccttt ttcattttca acttttgata atttatagtc aattaccttt gaatctaatt
      661 tatttact
//
LOCUS       DATTQF010000284          664 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.292, whole genome shotgun sequence.
ACCESSION   DATTQF010000284 DATTQF010000000
VERSION     DATTQF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.292"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(192..548)
                     /locus_tag="VB241_002756"
     CDS             complement(192..548)
                     /locus_tag="VB241_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HEP1245955.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIIKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT          220 a          120 c           83 g          241 t
ORIGIN      
        1 tttatgcatg cgcaaagcgc aggcgaactg aagcgaagtg taggggcttt tatgcatgcg
       61 ctaaagcgca gggcatttgc aaataaactg gtatatttat tctcaaaaaa acagcagtaa
      121 gatgattttc aattagaaat atcttactgc tgcttttaat atcttatata ttactcaaaa
      181 ttaaagctta attagtgttt catgtgcatt ttgcccatgt catgaatttt catcattaaa
      241 ccatgtggta catcgtcatg atcaactttt tttactttaa taattttatc agaatcggct
      301 aatttataaa caattacaaa gtcgcctttt ttaactgaaa cgtctgcatc tgaaggtaat
      361 ttaacgttct tctcaatgct ttcatctttt tcactaatca ttttttcaat tgtagcattg
      421 tctttcataa ttccataata tgtatctttc tttccaccag tcatcatcat gattactagt
      481 acaatacata caatcaacat gatggcaatt aaactaatac caagaccaaa accttttttc
      541 tttttcacgg ttcatcaatc tccttaaatg tagttaattc tgttatacct aaaagtacaa
      601 cagtttgcaa atgcagtatt tatattacat aaaattaatt taaaaagaca caccttaatc
      661 acaa
//
LOCUS       DATTQF010000285          662 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.293, whole genome shotgun sequence.
ACCESSION   DATTQF010000285 DATTQF010000000
VERSION     DATTQF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.293"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            51..482
                     /locus_tag="VB241_002757"
     CDS             51..482
                     /locus_tag="VB241_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245956.1"
                     /translation="MVNENDFKAEFTDQDTGEKIIYDTNEVKASIAPVVAVLVGFIAK
                     QGLKKAIQKYGKTVVTSMIRTSPQVAAQAAKKLGYSATKHVSHGKKVFKKNSKGRPQY
                     ISVDKDGHRGGAWKGASSIKNLGSKKTRSGTYDANLKRIGD"
     gene            484..>662
                     /locus_tag="VB241_002758"
     CDS             484..>662
                     /locus_tag="VB241_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP1245957.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQSPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTF"
BASE COUNT          267 a           92 c          119 g          184 t
ORIGIN      
        1 ataattataa taataaactt gataaaactt atgatgttaa atttttaagg attgttaatg
       61 aaaatgactt taaggcagaa tttaccgacc aagatacggg agaaaaaata atatatgata
      121 cgaatgaggt taaagcttca attgcacctg tagtggctgt attagtagga ttcatcgcca
      181 aacaaggact taagaaagct attcaaaaat atggtaagac agttgtaaca tcaatgattc
      241 gtacaagccc tcaagttgcc gcgcaggcag ctaaaaaatt gggttattct gctacaaagc
      301 atgtatccca tggcaaaaaa gtgttcaaga aaaatagtaa agggcgtcct caatatatat
      361 ctgtcgataa agatggacac cgaggaggag catggaaagg tgcaagcagt attaagaatt
      421 taggtagcaa gaaaacaaga agtggtacat acgatgctaa cctaaaaaga attggagatt
      481 aaaatgatta ttaaccttga aacacaacaa atacattttg atttagaaca tgaaattccc
      541 tattttcaat cacctgagaa aaataggata cgcttagata ttgatgttct caataaaaag
      601 caaatttcta atattattaa tgttatattt aataaccaat caaagacgaa atgtacattt
      661 tt
//
LOCUS       DATTQF010000286          642 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.294, whole genome shotgun sequence.
ACCESSION   DATTQF010000286 DATTQF010000000
VERSION     DATTQF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.294"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(21..248)
                     /locus_tag="VB241_002759"
     CDS             complement(21..248)
                     /locus_tag="VB241_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245958.1"
                     /translation="MLIEERGLKMTELNEIINAIQSLFESQSGYKISKNSGVPYQTVQ
                     DLRNGKTKIEDARFRTIIKLYSYYTSLKEQS"
     gene            complement(205..351)
                     /locus_tag="VB241_002760"
     CDS             complement(205..351)
                     /locus_tag="VB241_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245959.1"
                     /translation="MKLFYIAFLIIIWLNIFLGNEVIHTLTVLITTLYIVNRRKRVKN
                     DRVE"
BASE COUNT          213 a          107 c           56 g          266 t
ORIGIN      
        1 ctctttattt aatgatttaa ttatgactgc tcttttaatg atgtataata actatatagt
       61 tttataattg ttctaaatct agcatcttct attttcgttt taccattcct caaatcttgt
      121 acggtctgat acggcacccc tgaatttttc gatattttgt agcctgattg agactcaaac
      181 aaagattgaa tagcgttgat aatttcattc aactctgtca tttttaaccc tctttcttct
      241 attaacaata tacaatgttg ttattaaaac agtcagtgta tgaatgactt catttcctaa
      301 aaatatattc agccatataa taattagaaa tgcgatataa aatagtttca ttataatccc
      361 ctatcatttt tatatttatc actttccact tagtttcaac attaaaagct aacctaagtt
      421 taagattctc ttataacgcc tataactaga agtctacatt ttcccttgta taacatattt
      481 tacaatgttt tataatatta cactacaccc ttttacattt ttatacgaaa ttacaatact
      541 ttgttaaaat taattcgcac aaatattcca agacaattaa tttttctcta ttcataattg
      601 attaattata ctagttaata tacagttaac ttttatttaa ta
//
LOCUS       DATTQF010000287          628 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.295, whole genome shotgun sequence.
ACCESSION   DATTQF010000287 DATTQF010000000
VERSION     DATTQF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.295"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..268
                     /locus_tag="VB241_002761"
     CDS             <1..268
                     /locus_tag="VB241_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEP1245960.1"
                     /translation="PEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPN
                     IEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            325..>628
                     /locus_tag="VB241_002762"
     CDS             325..>628
                     /locus_tag="VB241_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP1245961.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKSPKDMAHNVDQLIEFIEKYDGEN"
BASE COUNT          248 a           73 c          117 g          190 t
ORIGIN      
        1 gccggagatt gatattttaa aagtaggaca tcatgggagt aagacaagta gttctaaaga
       61 atttatagag atgattaagc ctaaaataag tttgatttct tctggaaaga acaatatgta
      121 tcatcttcct aacatagaag ttgttaaacg attgcaaagg attcgcagtc gcatttacaa
      181 tagccaacaa aacggtcaag ttacaataga cttagatgat aatttaaaag ttgattcaaa
      241 ctcttatgga aatgcaagtg gtttatagat acagaaacat gatataatct attgatgtgt
      301 caaacgaatg aaagggtgta ctgaatgagc gacaatattg tagctattta tggagatgtg
      361 cctgaattgg ttgaaaaaca aagtgcagaa atcatatcac aatttttgaa aagtgataga
      421 gatgacttta actttgtgaa atataattta tacgaaacag agattgcacc aattgttgaa
      481 gaaacattaa cattgccttt cttttcagat aaaaaagcaa ttttggttaa aaatgcatat
      541 atatttacag gtgaaaaatc gccaaaagat atggctcata atgtagatca attaatagaa
      601 tttattgaaa aatatgatgg cgaaaatt
//
LOCUS       DATTQF010000288          626 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.296, whole genome shotgun sequence.
ACCESSION   DATTQF010000288 DATTQF010000000
VERSION     DATTQF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.296"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            188..394
                     /locus_tag="VB241_002763"
     CDS             188..394
                     /locus_tag="VB241_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245962.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGNTKI
                     KDAKFKTIIKLYEYQKSSENNSEC"
BASE COUNT          268 a           70 c           88 g          200 t
ORIGIN      
        1 aaataatata atatagaata gattgagaca agaaatttta tcaatatata ataaaaaacg
       61 ccaatactat taggcattag tttatgaatt aactagtttg gcaactacaa agaaacaatg
      121 tattgctaaa cgtagttact gcattaggat aattataatt ataaatagaa agagagcgtg
      181 attaaaaatg aatgagattg aaactattat aagtgaaata gaaaagttat taactaacaa
      241 tacaccatat agtatttcaa aaaactcagg tgtaccacgt caaacagtta ctgatttaaa
      301 ggtaggtaat actaaaataa aggatgccaa atttaaaaca ataatcaagt tatatgagta
      361 tcaaaagtcg tcagaaaata attcagagtg ctaataaata taccttgttt gattagatgg
      421 acaagagatt aaatttctat taatttcatg attgtgtgtc cacactttgt tcacctcaat
      481 aaaattatct ctaggttttt aaaattgtac atgtttaaac aatcaaaagt atgcattatt
      541 atattatcat ttccataaaa atttaaatga tctttaagtg atgatataga aggctgaaca
      601 cagtaaataa gttaacttta atgcag
//
LOCUS       DATTQF010000289          594 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.297, whole genome shotgun sequence.
ACCESSION   DATTQF010000289 DATTQF010000000
VERSION     DATTQF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.297"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            88..381
                     /locus_tag="VB241_002764"
     CDS             88..381
                     /locus_tag="VB241_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEP1245963.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
BASE COUNT          203 a           72 c           97 g          222 t
ORIGIN      
        1 aaaaacactt ttagattatt tttactcatt ctctatttta tttatttttg ctataataaa
       61 taaagaatat taaatggagg tgtttttatg aaaaagaaat ttgtttctag ttgtattgct
      121 agtacaatct tattcggcac tttattaggt gtaacttata aagctgaagc tgcaacagta
      181 catgtagctg gtggtgtttg gagccatggt attggaaaac attacgtatg gtcttattat
      241 agtcacaata aaagaaatca tggttcaaca gcagtaggga aatattcatc ttttagtggt
      301 gttgctcgac ctggtgttca atctaaggca tctgctccaa aggcttgggg tggaaacaaa
      361 acattttata gtcttcacta attacatata ttaattaagg agaatagaca gaattaagta
      421 aacttaattc tgtctattct cttgaaagag gcaatatgaa aaaattcaaa ttatttttag
      481 attttagtac tttattatta atttcagggc tattattttt atttttcttc aaagaaaatg
      541 aagaaattat acctgaaagt tccaatattt taacaatctc taattgggat aaat
//
LOCUS       DATTQF010000290          583 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.298, whole genome shotgun sequence.
ACCESSION   DATTQF010000290 DATTQF010000000
VERSION     DATTQF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.298"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(63..>583)
                     /locus_tag="VB241_002765"
     CDS             complement(63..>583)
                     /locus_tag="VB241_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245964.1"
                     /translation="ATYSQAVAYLLNKYGAVTDDYYKEKSYTRFLNGEIKSISKGKYT
                     RASEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENLVYCDLIEHLILHAIITKE
                     SNGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARAYLPGILVEKLLIKIDDML
                     KGIEIYDFLESR"
BASE COUNT          178 a           98 c           90 g          217 t
ORIGIN      
        1 attacaggtt taaacatcta taacaaaatc tcaacagcaa aaaataaatc tccattaatc
       61 atttatctag actcaaggaa atcatatatt tctatgcctt ttaacatatc gtcaatttta
      121 atgagtaatt tctctactaa tattccaggc aaataggcac gtgctttggt ggcttgcatc
      181 catgctggtt ttggattata ttcgccaatg taccaatcaa tgactgttgg tttgatcatt
      241 tgacataatc cagccacacc aaattggcca ttggattctt ttgtaataat tgcatgtaaa
      301 attaaatgct cgattaaatc gcagtaaact aagttttctt tcttttgata attgtatgag
      361 tacttaaatt cggaaatgaa tcttaggtca gataagtttt ggaatttgtc ctcgcttatg
      421 tgatgacaat ataatccttc gctagctcta gtgtattttc cttttgaaat acttttgatt
      481 tctccattta aaaatcgtgt gtatgatttt tccttataat agtcatcggt tacagcgcca
      541 tatttattta aaaggtatgc tacagcttgc gagtaagtag cgt
//
LOCUS       DATTQF010000291          581 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.299, whole genome shotgun sequence.
ACCESSION   DATTQF010000291 DATTQF010000000
VERSION     DATTQF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.299"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            62..328
                     /gene="zapA"
                     /locus_tag="VB241_002766"
     CDS             62..328
                     /gene="zapA"
                     /locus_tag="VB241_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEP1245965.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            329..>581
                     /locus_tag="VB241_002767"
     CDS             329..>581
                     /locus_tag="VB241_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP1245966.1"
                     /translation="MIIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAF"
BASE COUNT          213 a           76 c           98 g          194 t
ORIGIN      
        1 atccagtcat attttataac gtgaatttag attttaaaaa aaggatcagg agaaattata
       61 aatggcacag tttaaaaaca aggtaaatgt atcaattaat gatcagcttt ttacaattgt
      121 tggggaagat aacccagagc acatacgata tgtagcacat ttagttgatg ataaaataaa
      181 agaattaggg tataaagcag caggtttaga tacttcaaga aaagcaatac taactgctgt
      241 gaatattatg catgaaaaag tactactaga agaagaaaat cgacgtttga aacaacaaat
      301 tcacaaattg cagcagcgtg agcaataaat gatcattgat tttatcataa tcattttctt
      361 tgtgtatttt gtcatcgttg gattcagacg aggtttttgg ttatctatga tacatttgag
      421 tgcaacgatt gtatcattgt ggattgccag tcaattttac aaatctattg tagaaagatt
      481 aattgtattt attccatatc ctaaaacaac agcatttaat acaacttttg cgtttcattt
      541 taatcatcta caaaatcgat ttgaagcgat tgtagctttt t
//
LOCUS       DATTQF010000292          580 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.300, whole genome shotgun sequence.
ACCESSION   DATTQF010000292 DATTQF010000000
VERSION     DATTQF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.300"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..>580)
                     /locus_tag="VB241_002768"
     CDS             complement(<1..>580)
                     /locus_tag="VB241_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEP1245967.1"
                     /translation="IQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQEET
                     LNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFWISS
                     EDQMYGMSENLKGNNINSRLVYIKEPITDLSLKDIQAHLKSLDADNEDIPFSGAFSIE
                     FRLSKQTITCTDYKYDEDDLALWNKVNPSFALH"
BASE COUNT          174 a          106 c           83 g          217 t
ORIGIN      
        1 atgtagcgcg aaggatggat tgactttatt ccacaatgca agatcgtcct catcgtactt
       61 ataatcagta catgtaatag tttgtttcga caatctaaat tctatagaaa atgctccgct
      121 gaacgggata tcctcattat cagcatctaa tgacttcaaa tgcgcttgaa tatctttgag
      181 tgataaatct gtaattggct cttttatata aacaagcctt gagtttatat tgtttccctt
      241 taaattttca gacataccat acatttgatc ttctgaacta atccaaaact gcaatatccc
      301 ttcatttaca ggaaataatt cttgttgttc tggaagatca ttcaaattaa tctgtgctaa
      361 caataccatt ggtacattat tttcatctaa tggtatatct ttaaaagatt ttaaaaatgg
      421 cattccacca atcttactct ggtttaatgt ttcttcttgc atctctttta gatccatttc
      481 aatacatggt agtactggta aattaccgtt ttctacagat tctacgaaac ggttatatgc
      541 tttttgctcc tttttagaaa gatgactaac gtcttgaatg
//
LOCUS       DATTQF010000293          568 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.301, whole genome shotgun sequence.
ACCESSION   DATTQF010000293 DATTQF010000000
VERSION     DATTQF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.301"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>568
                     /locus_tag="VB241_002769"
     CDS             <1..>568
                     /locus_tag="VB241_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP1245968.1"
                     /translation="ANEENPKIEDLCKKSSVDDIALHNIDKDYMTNRFTINESTVLTT
                     EKFLDIDLLFKNFTWLDGKSAEFKDLKVEFSSSEISKEYFGKTVDIYGVYYKAHCHGE
                     HQVKTACTYGGVTPHENNKLSEPKEIGVAVYKDNVNVNTFIVTTDKKKVTAQELDIKV
                     RTKLNNVYKLYDRMTSDVQKGYIKFHSHS"
BASE COUNT          228 a           61 c           97 g          182 t
ORIGIN      
        1 atgcaaatga agaaaatcct aaaattgagg atttgtgtaa gaagtcaagt gtagacgata
       61 ttgctctaca taatattgat aaagactata tgactaatcg ctttacaata aatgagtcaa
      121 ctgtattaac tacagaaaaa tttttagata ttgatttatt atttaaaaat tttacttggt
      181 tagatggaaa atctgctgaa tttaaagatt taaaagtgga atttagctca tcggaaattt
      241 ccaaagagta ttttggaaaa actgtagata tttatggtgt ctattataaa gcgcattgtc
      301 atggtgagca tcaagtgaaa actgcctgta cttatggcgg ggtaacacct catgaaaata
      361 ataaattaag tgaacctaaa gaaataggag tagctgtgta taaggataat gtaaatgtta
      421 atacatttat cgttactaca gataaaaaga aagttactgc acaagaactt gatattaaag
      481 taagaacaaa attaaataat gtatataaat tatatgatcg aatgactagt gatgtacaaa
      541 aaggttatat taaattccat tctcattc
//
LOCUS       DATTQF010000294          563 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.302, whole genome shotgun sequence.
ACCESSION   DATTQF010000294 DATTQF010000000
VERSION     DATTQF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.302"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(102..>563)
                     /locus_tag="VB241_002770"
     CDS             complement(102..>563)
                     /locus_tag="VB241_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP1245969.1"
                     /translation="DMNQSSQALGLVLGISSLGALIGSLSASFLLKLFNFGKLIVIIL
                     FLDTIFRLMLPFSTYIFILIPLLGLTYMTQSILNIAIITLRQKKCSEHMLGRVNSVFK
                     TMVFAFRAIGLFLGGILLENKGGFYALTISAVLFIPLVLYILKNRFYKVEE"
BASE COUNT          207 a           92 c           67 g          197 t
ORIGIN      
        1 acttttatta accaattcag attttaaata taattgttta tctatatttg ctgcaccaat
       61 acaaacaata ttttggctgt ttttcattaa ttttctcctt actattcttc tactttataa
      121 aatctatttt tcagtatata taacactaat ggtataaata acacagcgct gatcgtaagt
      181 gcataaaatc cacctttatt ttcaagtaat atgccgccta aaaaaaggcc tatagctctg
      241 aatgcaaaaa ccattgtttt aaaaacacta ttaacacgtc ctaacatatg ttctgaacat
      301 ttcttttgcc tcagtgtgat tatcgctata tttaaaatgg attgtgtcat atatgtcaac
      361 cctaaaagtg ggatgagtat aaaaatataa gtactaaatg gtaacattaa tctaaaaatt
      421 gtatctaaaa ataatataat tacaattagt tttccgaaat taaataattt caagagaaaa
      481 cttgcagata atgaaccgat tagcgcacct aatgatgaga tacctaaaac taaccccaat
      541 gcttgagatg attgattcat gtc
//
LOCUS       DATTQF010000295          557 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.303, whole genome shotgun sequence.
ACCESSION   DATTQF010000295 DATTQF010000000
VERSION     DATTQF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.303"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            5..370
                     /locus_tag="VB241_002771"
     CDS             5..370
                     /locus_tag="VB241_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HEP1245970.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
BASE COUNT          218 a           58 c           94 g          187 t
ORIGIN      
        1 cataatgaag aagaaaaaaa acgcgattaa agttatcatt atattattta tttcattaat
       61 tgttgctgta gcatttttct ttggtctgaa aacatatcaa ggccataaga atattcaact
      121 tattgacagt tacttggaag agaaaaatct aaaagataaa attaaatcag aaaaaacaga
      181 gtatagtgct aaaaaaggtc tcttctataa ggaagttacg tttaaagatg aacctggtgt
      241 aacttatgtt gtgcaaccaa tcagtactaa taaaggatta tttgttgaag gctttgatac
      301 agagactaag aaaagtttga aaactgcaaa gcataaatat ttcaatcaga attataaacc
      361 atcaaaatag tgaaacgtat aactggtgaa acagtttagt gcctgcaatt ttaattattt
      421 aaggtaaaca gtttttgtat ggaaattagt gatttttgac atctataaaa gataaatatt
      481 ttaatttttt atagataatt atcaattttc tgatataatt gagtgatgtc agatgaaggg
      541 gagtttgtac tatggct
//
LOCUS       DATTQF010000296          540 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.304, whole genome shotgun sequence.
ACCESSION   DATTQF010000296 DATTQF010000000
VERSION     DATTQF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.304"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>540
                     /locus_tag="VB241_002772"
     CDS             <1..>540
                     /locus_tag="VB241_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP1245971.1"
                     /translation="GAITFFLLEQFNTMQNMGLVEKIGNSFFQSITTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KYIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLL"
BASE COUNT          188 a           76 c           78 g          198 t
ORIGIN      
        1 taggagctat tacattcttt ttattagaac agtttaatac tatgcaaaat atgggactag
       61 ttgaaaaaat cggaaattct tttttccaat caataacaac acgaacagcg ggctttaaca
      121 gtattgatat agcaagcatt aacaaatcta ctgctttaat gttaatgcta cttatgttta
      181 ttggtggtgc ccctctcagt gcagctggag gaattaaaat aactactttt gcagtagcgt
      241 ttatttttgt attaaattat atacgtaaag aaaataatgt ttcagtattc aataaagaaa
      301 tatctgacaa atatataaaa ctatctattg ttaccattaa tatctcattt ctatttatca
      361 gtatcattac ttttatatta tcgataatta atccgaacat atcattaatc aagttattat
      421 tcgaagtggt ttctgcattt ggaacagtcg ggttaagtat gaaccttacc acagaatatc
      481 atggtattac taaaataatt attatattcg ttatgctttg cggtaaagta ggactattaa
//
LOCUS       DATTQF010000297          538 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.305, whole genome shotgun sequence.
ACCESSION   DATTQF010000297 DATTQF010000000
VERSION     DATTQF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.305"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            455..>538
                     /locus_tag="VB241_002773"
     CDS             455..>538
                     /locus_tag="VB241_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP1245972.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDS"
BASE COUNT          225 a           63 c           68 g          182 t
ORIGIN      
        1 gtgaaactac ttatgaaata aaaaattttc taacttcaaa tattagagtt gatttagaaa
       61 ctgaactttt taaagacaca ttacttgaag attctaattt atctgattat ataaagaacc
      121 aacttccaac gatatggaga gactaaaaaa tagtttaaat ttatttaatt tcaataaaaa
      181 ctcataataa tatttaagta agttagattg cttttagcat taggaattcg tttataatta
      241 gatgttcaat ataggctttt ttacacatac atgaactata aaaagcggat aaataggagg
      301 tcattgcgaa atttgttaaa tacaaaattg atgagttcca cctaataaaa ttgttggttt
      361 tatagtaaca tctattttcg taccattcat gttaatttca gtgctaataa tgctgatata
      421 aagaagaata aattgattag gagaacacta tttaatgtct aaaaatatca atcaagcaaa
      481 tagtaaatta aatacgtcaa acaaaaaact aaaacaagct tattcaaaat ccgatagt
//
LOCUS       DATTQF010000298          532 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.306, whole genome shotgun sequence.
ACCESSION   DATTQF010000298 DATTQF010000000
VERSION     DATTQF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.306"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>532
                     /locus_tag="VB241_002774"
     CDS             <1..>532
                     /locus_tag="VB241_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEP1245973.1"
                     /translation="NAQAKQIIRLFNAETSQLINKVNSKNIESMQNKIFKSYEGINKI
                     FETDNVRIPETLLDIKLEMLDLMHKYQVKIEDEKIIRREERARLKEIEQAEKEMEKKL
                     KELDKDIRHHNNEIKKLTMYLNNTDLQVEKELYIEKIRELDQSLKNLNSERENVEDRK
                     DNAQSGFVYIISNIGS"
BASE COUNT          256 a           58 c           88 g          130 t
ORIGIN      
        1 aaaatgcaca ggccaagcaa ataatacgat tatttaatgc tgaaacttct caattaataa
       61 ataaagtaaa tagcaaaaat atcgaaagca tgcagaacaa aatatttaaa agttatgaag
      121 ggataaataa aatatttgaa acggataatg tacgtattcc tgaaacttta cttgacataa
      181 aacttgagat gttagattta atgcacaagt atcaagttaa gatagaagat gaaaagatca
      241 taagaagaga agaacgagct agactgaaag aaatagaaca agcagaaaaa gaaatggaga
      301 aaaaactaaa agagcttgat aaagacatta gacaccacaa taatgaaatc aaaaagctga
      361 caatgtattt aaataacaca gatttacaag tcgaaaaaga attatatatt gaaaagatta
      421 gagaattaga tcaatcactc aagaatttaa attctgaaag agaaaatgta gaggacagaa
      481 aagataatgc tcaatcaggg tttgtttata taatatcaaa tatcggttca tt
//
LOCUS       DATTQF010000299          528 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.307, whole genome shotgun sequence.
ACCESSION   DATTQF010000299 DATTQF010000000
VERSION     DATTQF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.307"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..167)
                     /locus_tag="VB241_002775"
     CDS             complement(<1..167)
                     /locus_tag="VB241_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP1245974.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITL"
BASE COUNT          182 a           87 c           68 g          191 t
ORIGIN      
        1 agagttatat agttatctat attaaaacca cttcttaact catttaactt gtctagtcca
       61 gcagaattag cttcacgctt tgttctaaaa cctttcttac ggtatctttt tccttcatac
      121 ttaaattcat attgccattt tttaccatcg taacaacgtg ttttcatgcg ttccctcctc
      181 aaaattggca aaaaataata agggtaggcg ggctacccat gaaaattgta taaaaaaaga
      241 cgcctgtata aaatacagac gccacttata gttataagat tacatggtta attaccaaaa
      301 atggtaacga atatatacgt gttttaaagg ataaaccttt aatatattaa aattatatca
      361 tcttatatca ggaatctgca atatattatt attaattcta tttatcagta acataatatc
      421 cgaagaatct attactggat ttttaatttt ttggggtaaa acttttctta tgcgaaactt
      481 actaatcggc tggaaagaat ttatgcaagc gtaactatta ccttttaa
//
LOCUS       DATTQF010000300          527 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.308, whole genome shotgun sequence.
ACCESSION   DATTQF010000300 DATTQF010000000
VERSION     DATTQF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.308"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>527
                     /locus_tag="VB241_002776"
     CDS             <1..>527
                     /locus_tag="VB241_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1245975.1"
                     /translation="QLTLPIETSVRIPQNDISRYVNEIFETIPDSEFDEFRHHRGATS
                     YHPKMMLKIILYAYTQSVFSGRRIEKLLHDSIRMMWLAQDQTPSYKTINRFRVNPNTD
                     ALIESLFIQFHSQCLKQNLIDDKSIFIDGTKVEASANRYTFVWKKSIQNYESKLNENS
                     KALYRDLVEEKIIPE"
BASE COUNT          198 a           79 c           79 g          171 t
ORIGIN      
        1 caacttacac taccaataga aacttctgtt agaattcctc aaaatgatat ttcgcgatat
       61 gttaatgaaa tttttgaaac tatacctgat agcgaattcg atgaattcag acatcatcgt
      121 ggtgcaacat cctatcatcc aaaaatgatg ttaaaaatca tcttatatgc atatactcaa
      181 tctgtatttt ctggtcgtag aatagagaaa ttacttcatg acagtattcg aatgatgtgg
      241 ttagctcaag atcaaacacc ttcttataaa actattaatc gttttagagt gaatcctaat
      301 actgatgcgc taattgaatc tttatttatt cagttccata gtcaatgttt aaagcaaaat
      361 cttattgatg ataaatcaat ttttattgat ggtacaaaag tagaagctag tgccaataga
      421 tatacatttg tgtggaagaa aagtattcaa aattacgaat cgaaattgaa cgaaaattca
      481 aaagcattat atcgtgactt agtcgaagaa aaaataatac cagagat
//
LOCUS       DATTQF010000301          524 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.310, whole genome shotgun sequence.
ACCESSION   DATTQF010000301 DATTQF010000000
VERSION     DATTQF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.310"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..137)
                     /locus_tag="VB241_002777"
     CDS             complement(<1..137)
                     /locus_tag="VB241_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEP1245976.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LS"
BASE COUNT          183 a           96 c           56 g          189 t
ORIGIN      
        1 gacaaacgta atgtattagt atcttgctct tttaatttat tacacatcgc aaaaaataaa
       61 ttaatttcag tagctgtaaa tcgccttaat ggaactaaat tcatttcatt tttatataca
      121 actgtctctc ctgtcatgat acagcccccc ctttattacg aacctaaagg agtatcgttt
      181 gaatatacac tcccatttga aaacttttat tcacaataaa ctactttatt atcaatgtca
      241 aaccataaaa ggagtacaac tttttaaaac actcctttta tgtggcaaaa acactccttt
      301 aggcattgaa atcccttgtt gatctaggtc tgaacaagcc cctaaaaagg tttttaaaaa
      361 gatttataaa aatatattaa aaagttagag gggggctacc tatttttcat cctaaaaaat
      421 aattaacctc aaacaaatgc atttaaatat ctatttttat aatgtataac catcttctct
      481 atcttcttta aaaatgattt ctcattcttg tgcttgatat ttct
//
LOCUS       DATTQF010000302          524 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.311, whole genome shotgun sequence.
ACCESSION   DATTQF010000302 DATTQF010000000
VERSION     DATTQF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.311"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            94..>524
                     /locus_tag="VB241_002778"
     CDS             94..>524
                     /locus_tag="VB241_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSau1 family transposase"
                     /protein_id="HEP1245977.1"
                     /translation="MSYNHLTLTERARIEVLRQENYSLRSIARKLKRSVSTISREISR
                     NNLNQSYQAETAQKNYETKRKLCGRPTRFTPELGNIIKYYLKCHWSPEQIVGRLLQNQ
                     ICFKTIYRWINSNMINFELISCLRQKGKRQKPKETRGKFNIG"
BASE COUNT          205 a           94 c           74 g          151 t
ORIGIN      
        1 gcaacaccta taaaaattca taaaaaatgc ccataattgg cactttgttg taagatgtta
       61 ataaccacaa aaacacatac aaggagtgcc aacatgagct ataaccatct tacactaact
      121 gaacgcgcac gtatagaggt tttacgtcaa gaaaattatt ctttaagatc tattgcacgt
      181 aaattaaaac gatctgtatc tactatctca cgtgaaatta gtaggaataa tttaaatcag
      241 tcatatcaag ctgaaacagc tcaaaaaaat tatgaaacca aaagaaaact ttgtggtcgt
      301 cctactcgat ttactccaga acttggtaat atcattaaat attatttaaa atgccactgg
      361 tctcctgaac aaatcgtagg tcgtctatta caaaaccaaa tctgttttaa aaccatctat
      421 cgatggatta actctaatat gattaatttc gaacttattt catgccttcg tcaaaaagga
      481 aaaagacaga aacctaaaga aactagaggt aagtttaata ttgg
//
LOCUS       DATTQF010000303          520 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.312, whole genome shotgun sequence.
ACCESSION   DATTQF010000303 DATTQF010000000
VERSION     DATTQF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.312"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            124..>520
                     /locus_tag="VB241_002779"
     CDS             124..>520
                     /locus_tag="VB241_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1245978.1"
                     /translation="MKCIDKTWVSYYKELADKLTDYQNKRYELIEIVKEVYKKTGIKF
                     PTLASDNVLMDIDPFTIFALFNKNSMRETNKVKILTELASELNIKSKIPSVFDSIPTV
                     NNLNATYYNFKDYGKDNDIETLWTLFTAAL"
BASE COUNT          208 a           53 c           84 g          175 t
ORIGIN      
        1 aatttattaa tctagtaaac taatagattt atagaaaatt ttatttgcaa ggggataatt
       61 ttgaaaagta gtattttcta tctttccata atacattgta attacaacgg aggggatatt
      121 gtgatgaagt gtatagataa aacgtgggtt agctattata aagaattagc tgataagtta
      181 acagattatc aaaataaacg ttatgaatta attgaaatag tgaaggaagt atataaaaaa
      241 acgggaataa aattccctac tttagcaagt gataatgtat tgatggacat agatcctttt
      301 acaatatttg cattatttaa taaaaattcc atgagagaaa ctaataaggt aaaaatatta
      361 acagaattag cttcggaatt gaatattaag tccaaaattc cgtcagtttt tgacagtatt
      421 ccaacagtca ataatctgaa tgctacatat tataatttta aagattatgg aaaagataat
      481 gatatagaga ccttgtggac cttatttact gctgcattaa
//
LOCUS       DATTQF010000304          517 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.314, whole genome shotgun sequence.
ACCESSION   DATTQF010000304 DATTQF010000000
VERSION     DATTQF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.314"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..241
                     /locus_tag="VB241_002780"
     CDS             <1..241
                     /locus_tag="VB241_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP1245979.1"
                     /translation="YINVRFYENSDTIKVIENNKIRFLKLKVGKIINVAKVDIIAFDK
                     KGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            445..>517
                     /locus_tag="VB241_002781"
     CDS             445..>517
                     /locus_tag="VB241_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP1245980.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHV"
BASE COUNT          223 a           55 c           79 g          160 t
ORIGIN      
        1 atatatcaat gttagatttt atgaaaacag tgacactatt aaagtaatag agaataataa
       61 aatacgattt ctaaaactaa aagtaggcaa aataattaat gttgcaaaag ttgatattat
      121 tgcctttgat aaaaaaggga atatcaataa atttggtagc ataacaattg ataataacaa
      181 ttcaatttat agaataatat tccatattga aaaaggaaga attcgttatg aaaagctata
      241 agtgtaaagg atcattctta atagatagta tggctggatt tttgcttatt ggattgatta
      301 cattactatt gataccaatg atgaatcaaa tgcaagcgag tataaaccat aaactacaaa
      361 caattgatgc ttctaaagta attttgacga ctgtatctaa aattaataaa gaagaactta
      421 agaagggggt aactataggg aaatatgata ttaagcaaag tgaccaacaa atttgtgcta
      481 tttcaaaaaa taccacttct tatcaaaaga catgtat
//
LOCUS       DATTQF010000305          506 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.315, whole genome shotgun sequence.
ACCESSION   DATTQF010000305 DATTQF010000000
VERSION     DATTQF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.315"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..426
                     /locus_tag="VB241_002782"
     CDS             <1..426
                     /locus_tag="VB241_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP1245981.1"
                     /translation="KVDFYRYTLGYLFQNFALLENQTISQNLGLALKFKKNKKDNMNL
                     KKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLDTKNGKI
                     VIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
BASE COUNT          202 a           54 c           89 g          161 t
ORIGIN      
        1 aaagtcgatt tttatagata tacgttaggg tatttgtttc aaaattttgc tttacttgaa
       61 aatcaaacaa tcagtcaaaa tttaggttta gccctaaagt tcaaaaagaa taaaaaagat
      121 aatatgaact taaagaaaga agtattaaaa aaggtcggcc tagatttaga tatcaagaga
      181 attgttagtt ctctaagcgg tggtgaacaa caaagagtag cattggcaag attaatatta
      241 aaagatccga aaatcatttt agctgatgaa cctactggat cactagatac gaaaaatgga
      301 aaaatagtta ttgatttatt attgaagcta cttgatgaaa ataaaactat gattgtcgta
      361 acgcatgatt tagagcttgc gaaacgattt gatgtcatag tcaatatttc tgagcttaga
      421 agttaagaaa tattgatagg tatagccata catagagaaa aaacaatatg aaatatgatt
      481 agtcattact tttgagtttt ttaatt
//
LOCUS       DATTQF010000306          504 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.316, whole genome shotgun sequence.
ACCESSION   DATTQF010000306 DATTQF010000000
VERSION     DATTQF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.316"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..>504)
                     /locus_tag="VB241_002783"
     rRNA            complement(<1..>504)
                     /locus_tag="VB241_002783"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          107 a          154 c          106 g          137 t
ORIGIN      
        1 tctctagagt tgtcaaagga tgtcaagatt tggtaaggtt cttcgcgttg cttcgaatta
       61 aaccacatgc tccaccgctt gtgcgggtcc ccgtcaattc ctttgagttt caaccttgcg
      121 gtcgtactcc ccaggcggag tgcttaatgc gttagctgca gcactaaggg gcggaaaccc
      181 cctaacactt agcactcatc gtttacggcg tggactacca gggtatctaa tcctgtttga
      241 tccccacgct ttcgcacatc agcgtcagtt acagaccaga aagtcgcctt cgccactggt
      301 gttcctccat atctctgcgc atttcaccgc tacacatgga attccacttt cctcttctgc
      361 actcaagttt tccagtttcc aatgaccctc cacggttgag ccgtgggctt tcacatcaga
      421 cttaaaaaac cgcctacgcg cgctttacgc ccaataattc cggataacgc ttgccaccta
      481 cgtattaccg cggctgctgg cacg
//
LOCUS       DATTQF010000307          502 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.317, whole genome shotgun sequence.
ACCESSION   DATTQF010000307 DATTQF010000000
VERSION     DATTQF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.317"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            334..438
                     /locus_tag="VB241_002784"
                     /pseudo
     CDS             334..438
                     /locus_tag="VB241_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          215 a           49 c           73 g          165 t
ORIGIN      
        1 ccttattcct ttgacgtact gatttataat acattttttt cttttttaaa ttatataatc
       61 atgtcttaac tcaatcttcg attggaagtt atttaattga gacatgacat ttatgaagga
      121 atgctatttt acattatgaa aggtattaaa ccaaattatg ctgagcttgg tcgacaatat
      181 aattgtgatt caagaacagt taaaaaatat tatgaagcag gaaaataaaa tgaattagaa
      241 cgattgaaga aaagacaaca aaataagaaa gcatcaaagc tagattcatt taaagaaatt
      301 aataataaaa aaattgaatt aggatataca gctatggcca tttttaaata tatggagaaa
      361 aagggttatg aaggtaaata taatattcta gaagaatact gtaaaaataa aatacaaaat
      421 gagactaaaa aaataacatt tttttgaaga tagtataaat atttgtttct tagataacag
      481 tgttgttggt aaaacacacc ta
//
LOCUS       DATTQF010000308          500 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.318, whole genome shotgun sequence.
ACCESSION   DATTQF010000308 DATTQF010000000
VERSION     DATTQF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.318"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>500
                     /locus_tag="VB241_002785"
     CDS             <1..>500
                     /locus_tag="VB241_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEP1245982.1"
                     /translation="NKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEII
                     SKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAIL
                     NGAKQIETTFLGIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQ
                     ISENKP"
BASE COUNT          201 a           64 c           82 g          153 t
ORIGIN      
        1 caaataaata ttatattcag aaaatcaaag actgcttgga tatattaaag aaagataaaa
       61 aaggagtaga tatttgtttt gaagatgcaa caaggacttc tagagaaaaa ttgaaagaat
      121 acatggaaat tatttcaaaa tatcaagtta gaacagttac atttgcggac actgtaggat
      181 gttcgacacc attagaatac ggagatattt ttaattactt tgtaaaaaaa tattctaaca
      241 taattttttc tgctcattgt cataacgatc tagggttggc tactgcaaat acattagctg
      301 caattttaaa tggtgcgaag caaatagaaa ctacattttt gggaattggt gagagagcgg
      361 gtaatgctcc tattgaggaa ataattacta ttttgacaaa aaaacaaata gaaagtacgg
      421 aattcacttt acccgacgta tataaaacta gtattaatat ttctaaaatt ctcgattttc
      481 aaatatcaga aaacaaacct
//
LOCUS       DATTQF010000309          498 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.319, whole genome shotgun sequence.
ACCESSION   DATTQF010000309 DATTQF010000000
VERSION     DATTQF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.319"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..497
                     /locus_tag="VB241_002786"
     CDS             <1..497
                     /locus_tag="VB241_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEP1245983.1"
                     /translation="MTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIELN
                     YYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPKD
                     YKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKSV
                     MKEF"
BASE COUNT          217 a           64 c           82 g          135 t
ORIGIN      
        1 caatgacata tagtgataat acagcaaaca ataaaattat aaaagaaatc ggtggaatca
       61 aaaaagttaa acaacgtcta aaagaactag gagataaagt aacaaatcca gttagatatg
      121 agatagaatt aaattactat tcaccaaaga gcaaaaaaga tacttcaaca cctgctgctt
      181 tcggtaagac tttaaataaa cttatcgcaa atggaaaatt aagcaaagaa aacaaaaaat
      241 tcttacttga tttaatgtta aataataaaa gcggagatac tttaattaaa gacggtgttc
      301 caaaagacta taaggttgct gataaaagtg gtcaagcaat aacatatgct tctagaaatg
      361 atgttgcttt tgtttatcct aagggccaat ctgaacctat tgttttagtc atttttacga
      421 ataaagacaa taaaagtgat aagccaaatg ataagttgat aagtgaaacc gccaagagtg
      481 taatgaagga attttaat
//
LOCUS       DATTQF010000310          498 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.320, whole genome shotgun sequence.
ACCESSION   DATTQF010000310 DATTQF010000000
VERSION     DATTQF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.320"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            245..327
                     /locus_tag="VB241_002787"
     tRNA            245..327
                     /locus_tag="VB241_002787"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Leu,seq:gag)
     gene            349..422
                     /locus_tag="VB241_002788"
     tRNA            349..422
                     /locus_tag="VB241_002788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:381..383,aa:Gly,seq:tcc)
BASE COUNT          185 a           77 c           84 g          152 t
ORIGIN      
        1 cgttccttaa aaaagaacaa attaaataaa ctttgatttg aaagacagtc aattctaaat
       61 ttaatttata aaatatgttg acaataagtt cttaatgttt tattatgaat aacgttgtgt
      121 taacaactga taacacaaat taatttaatg taaaaaaacg attaaaagtt attgacaaat
      181 atttttaatc ataatataat aaataacgtt ctgaaaaacg aagatgtttt aaatgcctat
      241 atccgcggtc gtggcggaat ggcagacgcg ctaggttgag ggcctagtgg gagaaatccc
      301 gtggaggttc aagtcctctc ggccgcatca aaattcttaa ttaaataagc gggtgtagtt
      361 taatggcaaa acctcagcct tccaagctga tgttgtgggt tcgattccca tcacccgctc
      421 cataaataat tttaatgaac attgaaaact gaatgacaat atgtcaacgt taattccaaa
      481 aaacgtaact ataagtta
//
LOCUS       DATTQF010000311          497 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.321, whole genome shotgun sequence.
ACCESSION   DATTQF010000311 DATTQF010000000
VERSION     DATTQF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.321"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..477)
                     /locus_tag="VB241_002789"
     rRNA            complement(<1..477)
                     /locus_tag="VB241_002789"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          111 a          143 c          103 g          140 t
ORIGIN      
        1 cagttactta cacatatgtt cttccctaat aacagagttt tacgatccga agaccttcat
       61 cactcacgcg gcgttgctcc gtcaggcttt cgcccattgc ggaagattcc ctactgctgc
      121 ctcccgtagg agtctggacc gtgtctcagt tccagtgtgg ccgatcaccc tctcaggtcg
      181 gctatgcatc gttgccttgg taagccgtta ccttaccaac tagctaatgc agcgcggatc
      241 catctataag tgacagcaag accgtctttc acttttgaac catgcggttc aaaatattat
      301 ccggtattag ctccggtttc ccgaagttat cccagtctta taggtaggtt atccacgtgt
      361 tactcacccg tccgccgcta acatcagaga agcaagcttc tcgtccgttc gctcgacttg
      421 catgtattag gcacgccgcc agcgttcatc ctgagccagg atcaaactct ccataaaaat
      481 tatgatgttt gattagc
//
LOCUS       DATTQF010000312          494 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.322, whole genome shotgun sequence.
ACCESSION   DATTQF010000312 DATTQF010000000
VERSION     DATTQF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.322"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..371
                     /locus_tag="VB241_002790"
     CDS             <1..371
                     /locus_tag="VB241_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245984.1"
                     /translation="SFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWFSFKT
                     IQLQCFNNSNWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIV
                     MPIGLRIERNISKKHSHTFL"
BASE COUNT          161 a           64 c           61 g          208 t
ORIGIN      
        1 ttagttttct caaaggattt ttaggatttt tcatggaaaa aagtggaagt aatttatatt
       61 ttgtttcatt cccgttttca gtagtcgttg gattctttat tgtcttttta tttatatggt
      121 ttagttttaa aacaattcaa ctgcagtgtt ttaacaattc aaattggata tggatattca
      181 cctatttagc aatcattcta ctaattgtag gatttttcta tgtattcttt attccacaat
      241 cattacttgc ctttggacca tatattcagg taagcaattg ggtatttatt atattttcat
      301 ttatagttat gcctattgga ttacgcattg aaagaaatat ttcgaaaaaa cattctcata
      361 catttttata aacaaaaagc ttattacatc atatgctaat ttggtactgt taatgataaa
      421 atcactaaat tacagttacc taaaaacata ttttgcaata agctttttat taattaatca
      481 ataaagcatc tata
//
LOCUS       DATTQF010000313          491 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.323, whole genome shotgun sequence.
ACCESSION   DATTQF010000313 DATTQF010000000
VERSION     DATTQF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.323"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(130..>491)
                     /locus_tag="VB241_002791"
     CDS             complement(130..>491)
                     /locus_tag="VB241_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP1245985.1"
                     /translation="TLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDILEDFKGVDRN
                     DIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKI
                     TITVKVGTNLQTKTIYV"
BASE COUNT          166 a           68 c           54 g          203 t
ORIGIN      
        1 gaaaatggaa aaataaattg ctgttagcat caataagaac ggcgtaacat atcctttttc
       61 tttatataaa tcaaaattaa taaattattt cacgtccttt ttaatagtac tcatttaaat
      121 tctagtaagt tatacataaa ttgttttagt ctgcagattt gtaccgactt taactgttat
      181 cgttatttta ataatagatt tatagtagat atttgcagta aatgctgtaa cattattaat
      241 cattgttata tttcctctgt catttacaac tttaataatt ttattattta ttaatttgta
      301 ttcgatcatt tcttcacctt tatgtaaaat gatacgctgt tgcctaattt caatatcgtt
      361 tctatctaca cctttaaaat cctctagaat atctcttgag aaaaattcga aatctacagt
      421 tgttaattcc ctactttcaa ttagaaaagt tttgttaagt ctaattaagt ctggaacaat
      481 tagtaaagtt a
//
LOCUS       DATTQF010000314          467 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.325, whole genome shotgun sequence.
ACCESSION   DATTQF010000314 DATTQF010000000
VERSION     DATTQF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.325"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>467
                     /locus_tag="VB241_002792"
     CDS             <1..>467
                     /locus_tag="VB241_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245986.1"
                     /translation="KTSLARGIKEIIEENNPDSLRYFYTDYIHELLLLEDSNKFKGVK
                     ATFGTKNVEIENKIIKLKNEVVDMTDTKKLLVEKRRKLRELINEIHKSRKGNLKIPLK
                     SSNKSIEEVIAIYEKNLKDAKKIEHNIESIRNFVANDETLYNKYNSIYEVMIP"
BASE COUNT          223 a           40 c           76 g          128 t
ORIGIN      
        1 aaaacttcat tagctagagg tattaaagaa ataatagaag aaaataatcc agatagcttg
       61 aggtattttt acactgatta tattcatgaa cttcttttac tagaagattc caataaattt
      121 aaaggggtaa aagcgacatt tggtacaaaa aatgtagaaa ttgaaaataa aataatcaag
      181 ctgaaaaatg aagttgttga tatgacggat actaaaaagt tactagttga gaagagaagg
      241 aagttaagag aactaataaa cgaaatacat aagtcaagaa aagggaatct taaaatacca
      301 ttaaaatcat caaataaaag tatagaagaa gtaatagcta tttatgaaaa aaatttaaaa
      361 gatgctaaaa aaatagagca taatatagaa agtataagaa attttgtagc gaatgatgaa
      421 acactttata ataaatataa tagcatttat gaagttatga ttccaag
//
LOCUS       DATTQF010000315          463 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.326, whole genome shotgun sequence.
ACCESSION   DATTQF010000315 DATTQF010000000
VERSION     DATTQF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.326"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            73..>463
                     /locus_tag="VB241_002793"
     CDS             73..>463
                     /locus_tag="VB241_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEP1245987.1"
                     /translation="MAYKYPDLIEYTCLNKKEYKAEDFKKRENNMAYLQHYGLRTTLI
                     DITENPFIALLFLTSNSQVFNNATLDMYNINPKIHSEQNLFSRVKMISKNKRIIAQKG
                     AFFNFEKLLIFQNEQNVNRDKINKIPLV"
BASE COUNT          205 a           56 c           66 g          136 t
ORIGIN      
        1 atgaaagcag gattattaag aaatgatata atagatgatt taaaaaaaga gtttgagaat
       61 atatacgaag atatggctta taagtaccca gatttaatag agtatacgtg cttaaataaa
      121 aaagaatata aagcagaaga ttttaaaaaa agagaaaaca atatggctta tttacaacat
      181 tatgggctaa gaactacatt aattgatatt actgaaaacc cctttatagc gctcctattt
      241 ttaacttcta actcacaagt gtttaacaat gcaaccttag acatgtataa tatcaatcct
      301 aaaatacaca gcgaacaaaa tttgttttca agagtaaaaa tgattagtaa aaacaaaaga
      361 ataatagctc aaaagggtgc gttttttaac ttcgaaaagc tattgatttt tcagaatgaa
      421 caaaatgtaa atagagataa aattaataaa atacctctag ttc
//
LOCUS       DATTQF010000316          458 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.327, whole genome shotgun sequence.
ACCESSION   DATTQF010000316 DATTQF010000000
VERSION     DATTQF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.327"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            198..374
                     /locus_tag="VB241_002794"
     CDS             198..374
                     /locus_tag="VB241_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP1245988.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT          189 a           61 c           69 g          139 t
ORIGIN      
        1 tctcattaaa taaattatta agagattttt agaacaaaat ctaaaagtct ttgtgatttt
       61 gatgtgaaat atgtttcaaa taaaaaacaa tttactaatt gaccataaat tacagatata
      121 ttatacttat aaatgcatag ttttactgtg caattgacta taaagttccg ttgatatttg
      181 gagggaggga aatacagatg tctaaaacag tagtacgtaa aaatgaatca cttgaagatg
      241 cgttacgtag atttaaacgt tcagtttcta aaagtggaac aatccaagaa gtacgtaaac
      301 gtgaatttta cgaaaaacca agcgtaaaac gtaaaaagaa atcagaagct gcacgtaaac
      361 gtaaattcaa ataattaata cctctgttga ctccctcaac acgaatatta attatataaa
      421 ataaacatca caagttagtg tctgacacta atatgtga
//
LOCUS       DATTQF010000317          451 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.329, whole genome shotgun sequence.
ACCESSION   DATTQF010000317 DATTQF010000000
VERSION     DATTQF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.329"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..322
                     /locus_tag="VB241_002795"
     CDS             <1..322
                     /locus_tag="VB241_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP1245989.1"
                     /translation="KEIINKTLKQLSRIELLIIDEICYSAITKEQADLFYQLMSLRYE
                     WKSTIITTNIPFSSWGESFSNKIVSAAIIDRLVHHSKVFKITGESYRLKDYKTEKSLN
                     IRQS"
BASE COUNT          192 a           65 c           56 g          138 t
ORIGIN      
        1 caaagaaatc attaataaaa cattaaaaca attaagcaga atagagttac ttatcattga
       61 tgaaatttgt tattctgcca tcaccaaaga acaagctgat ttattctacc agttaatgtc
      121 actaaggtat gaatggaaat caacaataat tacgactaat attccatttt cgagttgggg
      181 agagtcattt agtaataaga ttgtatcagc agctataatt gatcggttag ttcaccattc
      241 aaaagtattt aaaattactg gagaatcata tcgattaaaa gattataaaa ccgaaaaatc
      301 attaaatata cgacaatctt aaaccactaa aaaacgacat tttcaaacct ccataaaaca
      361 gcatttttaa accgctattg acattaagat tatataaaag gcaaatatta aagctaaaat
      421 tccgattaga taattaataa agattattat t
//
LOCUS       DATTQF010000318          446 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.331, whole genome shotgun sequence.
ACCESSION   DATTQF010000318 DATTQF010000000
VERSION     DATTQF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.331"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..420
                     /locus_tag="VB241_002796"
     CDS             <1..420
                     /locus_tag="VB241_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP1245990.1"
                     /translation="FWSIIAKLKEENRTILYTSHYIEEVERMSDKIILIENGEIKLND
                     STSHIRTNQQSQITLSDEYKRKLKPDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLH
                     INLDDIEIQKVSIVDSYFNNKKQRGSNYDTKLLENRI"
BASE COUNT          192 a           60 c           61 g          133 t
ORIGIN      
        1 ttttggtcaa taatagctaa gttaaaagaa gaaaatcgga cgattcttta tacatcgcac
       61 tatatagaag aagtagagcg catgtccgac aaaattattt tgatagaaaa tggagaaatc
      121 aaacttaatg attcaacgtc acatattaga accaatcagc aatctcagat tacgttatcc
      181 gatgaatata aaagaaagtt aaaaccagat aaagatgatt tagttattca aaaaaatcat
      241 aatggcacta tcaaaattat tacttcaaat gtaaatgata cgattttata tcttcaacaa
      301 cttcatatta atttggatga tattgaaata caaaaagtct caattgttga ttcatacttc
      361 aacaataaaa agcaaagggg atctaattat gatactaagt tacttgaaaa tcgaatttaa
      421 agttatactg cgtaaaaaaa caacat
//
LOCUS       DATTQF010000319          439 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.332, whole genome shotgun sequence.
ACCESSION   DATTQF010000319 DATTQF010000000
VERSION     DATTQF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.332"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            63..>439
                     /gene="sem"
                     /locus_tag="VB241_002797"
     CDS             63..>439
                     /gene="sem"
                     /locus_tag="VB241_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HEP1245991.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQNI
                     NPDNNRLSHQLVFSKDNSTVTAEFKNVEDVKKFKNRAVDVYGLSYSGYCLKNKYMYGG
                     VTLAGDYLEKSRCIPINLWVNGNL"
BASE COUNT          158 a           51 c           76 g          154 t
ORIGIN      
        1 atgttttttt acaataaaag gataataaag atagacatat ttaattatag gagaaatata
       61 aaatgaaaag aatacttatc attgttgttt tattgttttg ttattcacaa aatcatatcg
      121 caaccgctga tgttggagtt ttgaatctta ggaactatta tggtagctat ccaatcgaag
      181 accatcaaaa tattaatcct gacaataatc gtctttcaca tcaattagtt ttttctaagg
      241 ataattcgac agtaacagct gaatttaaga atgttgaaga tgtaaaaaaa ttcaaaaatc
      301 gtgctgttga tgtatacggc ctaagttata gtggatattg tctaaaaaat aaatatatgt
      361 acggaggagt tactttagcg ggtgattatt tagagaagtc tagatgtatt cctattaatc
      421 tttgggttaa tggcaacct
//
LOCUS       DATTQF010000320          436 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.333, whole genome shotgun sequence.
ACCESSION   DATTQF010000320 DATTQF010000000
VERSION     DATTQF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.333"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..>436
                     /locus_tag="VB241_002798"
     CDS             <1..>436
                     /locus_tag="VB241_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP1245992.1"
                     /translation="ISTDQLLNNTIILKDIDISVLKTSSLKVEFNSSDLANQFKGKNI
                     DIYGLYYGNKCVGLTEEKTSCLYGGVTIYDGNQLDEERVIGVNVFKDGIQQEGFVIKT
                     KKAKVTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSN"
BASE COUNT          189 a           43 c           70 g          134 t
ORIGIN      
        1 aaattagtac agatcaacta ctgaataata ctataatatt aaaagatatt gatatatccg
       61 tacttaaaac ttctagtttg aaagttgagt ttaactcatc agatttagca aatcaattta
      121 aagggaaaaa tatagatatt tatggactgt attatggaaa taaatgtgta ggcttaactg
      181 aagaaaaaac atcatgctta tacggaggag ttacgatata tgatggaaat caattagatg
      241 aagagagagt tataggcgtt aatgtattta aagatggtat ccaacaagaa ggttttgtta
      301 taaaaactaa aaaggctaaa gtaacagtac aagaattaga tactaaagtt cgatttaaat
      361 tagaaaattt atataaaata tacaataaag ataccggtaa catacaaaaa ggatgcattt
      421 tctttcattc taataa
//
LOCUS       DATTQF010000321          435 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.334, whole genome shotgun sequence.
ACCESSION   DATTQF010000321 DATTQF010000000
VERSION     DATTQF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.334"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..383
                     /locus_tag="VB241_002799"
     CDS             <1..383
                     /locus_tag="VB241_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HEP1245993.1"
                     /translation="IMGYMIRSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTK
                     SNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNG
                     SNVVTMDLALDKAVRDDIYRYITN"
BASE COUNT          186 a           44 c           77 g          128 t
ORIGIN      
        1 taatcatggg atacatgata agaagccaag aaatgccaat atttatcact tatgataagc
       61 atgaggatat aagtgatagc acaaaatatg aagatgaatt tttaagtcaa gatgaattaa
      121 aatggtttac taaatctaat agaacgctaa aatcaaaaga agttcagaaa attttgtccc
      181 atagagcaaa aggaattaaa atgtatattt ttgtacagaa aaaagatgat gatggtatat
      241 atttttatta tttaggaact gctggatata tagaaggttc agagaagcaa gataaaatgc
      301 cgaatggttc aaacgtagta actatggatt tagcactcga taaagctgtc agagatgata
      361 tatatcgcta tataacaaat taatgtaaac cgaaaaatga atgttagtaa agatgtattt
      421 aaacatcaat tttaa
//
LOCUS       DATTQF010000322          433 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.335, whole genome shotgun sequence.
ACCESSION   DATTQF010000322 DATTQF010000000
VERSION     DATTQF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.335"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            102..433
                     /locus_tag="VB241_002800"
                     /pseudo
     CDS             102..433
                     /locus_tag="VB241_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120900.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
BASE COUNT          171 a           54 c           79 g          129 t
ORIGIN      
        1 ctaaattatg tcattcactc aacgaaaaaa tatttaaagt taaaggtatg ataccaggtg
       61 tgaatgcgcc acctatacat ccttggtgta gaagtaccac agtgccagat gtcggtaatt
      121 ggcgagacaa gttctttaaa gagcgtgaag gtaaatatcg ggtagaaggt tcttttattg
      181 aatcgggtgc acttaataat aaaagtgatg aatacggtat caaaaggaac aggcatgctc
      241 aaatttatta taattcagtt agaaatcaag aaaacagata gagatttcta aaattgctaa
      301 aaatactaat ataaataaaa atacaattca aagagtttat gaacatatat ttgaaaataa
      361 gtatttatta gaaaatggat ttaaacaatt tgatcctgac ttttatatgg ctcaaagttg
      421 gcaacgttta aga
//
LOCUS       DATTQF010000323          426 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.336, whole genome shotgun sequence.
ACCESSION   DATTQF010000323 DATTQF010000000
VERSION     DATTQF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.336"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..138
                     /locus_tag="VB241_002801"
     CDS             <1..138
                     /locus_tag="VB241_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245994.1"
                     /translation="INKQIAKNLSLEGMYVTREQQIQILHAINNEKEITNELIRKIAF
                     K"
BASE COUNT          175 a           58 c           54 g          139 t
ORIGIN      
        1 attaataaac aaatcgctaa aaacctttca cttgaaggta tgtatgttac acgagaacag
       61 caaatacaaa ttcttcatgc aattaataat gagaaagaaa ttacaaatga acttattcgt
      121 aaaatcgctt ttaaataatg aaatatatag caacttttca aaaggcatta aactatcttg
      181 aaaagttgct tattaaataa taattgctca aataaaaaac atataaatgc tcttaccaaa
      241 aaaatattcg taactaaaat attacaaagt aattaaaaag tgtgattacc caaaaatcct
      301 ttttttattc agattgcctt aatattgctc ctaaatgttg ctgtgcagta gtaaataata
      361 cgaatgtaat taacttgtca atagcggttc aaaaatgctg ttttatggcg gtttgaaaat
      421 gtcgtt
//
LOCUS       DATTQF010000324          384 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.338, whole genome shotgun sequence.
ACCESSION   DATTQF010000324 DATTQF010000000
VERSION     DATTQF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.338"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            78..>384
                     /locus_tag="VB241_002802"
     CDS             78..>384
                     /locus_tag="VB241_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP1245995.1"
                     /translation="MEKNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKNAKENNDSNEVAYLLKGAKVTKVYGDQNSVSFVPGEKATELLFDS
                     "
BASE COUNT          166 a           52 c           75 g           91 t
ORIGIN      
        1 tgtccaaaac gtgctgatga cattttaaaa gcaagtatgg aatatcagtc gacagactat
       61 aaacggaggt atatctcatg gaaaaaaatg aaagtaatat tactgatgta actcagaacg
      121 aagagcaact agacaacagt gatgaacaat cacaacagaa tgagaaaaca ttttctcaag
      181 aggaagtatc acaattgatt aaagagcgta tagctagaga acgcaaaaaa tcagatgaac
      241 gtattaaaaa tgccaaagaa aacaatgata gcaatgaagt agcttattta ttaaaaggtg
      301 ctaaagttac aaaagtatat ggcgatcaaa atagtgtatc ttttgtgcca ggggaaaaag
      361 caaccgaatt attatttgac agta
//
LOCUS       DATTQF010000325          343 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.345, whole genome shotgun sequence.
ACCESSION   DATTQF010000325 DATTQF010000000
VERSION     DATTQF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.345"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..106)
                     /locus_tag="VB241_002803"
     CDS             complement(<1..106)
                     /locus_tag="VB241_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP1245996.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTLTS"
     gene            complement(84..>343)
                     /locus_tag="VB241_002804"
     CDS             complement(84..>343)
                     /locus_tag="VB241_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEP1245997.1"
                     /translation="VLLIISLLLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYAL
                     KHNRNPSSIEDLIADGFIKEEQKTCKSGETITISNGEAVAN"
BASE COUNT          109 a           58 c           43 g          133 t
ORIGIN      
        1 tagatgtcaa tgtcaaaagt aaaaatatac tgattaacat cattaccaca agcatctcaa
       61 tcatagtaaa tgctgactgt tttctaattt gcaactgctt ctccattact aattgttatt
      121 gtctctcctg atttacatgt cttttgctct tcttttataa aaccatctgc aattaagtct
      181 tcaatagacg atggatttct attatgtttc aatgcatacg cttcaatttg actattaacc
      241 atttttacct gtgcattaca acctgttgat tgtatgtgag cagtttgttt agcaatattt
      301 ggaatgatta aaattaataa taaactgatg attaataaca cta
//
LOCUS       DATTQF010000326          331 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.348, whole genome shotgun sequence.
ACCESSION   DATTQF010000326 DATTQF010000000
VERSION     DATTQF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.348"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            complement(<1..>331)
                     /locus_tag="VB241_002805"
     CDS             complement(<1..>331)
                     /locus_tag="VB241_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP1245998.1"
                     /translation="FYVLELIKKVLKSKFNQIFIFTHSWNDFCDITYRLKGEDHNFYE
                     VYKDHQGTSFLEKFKKVKTPYKKLFQEIYELSRKSQKDIVEEDCYYYHSINSIRRVFE
                     EFLSFKLK"
BASE COUNT          113 a           53 c           28 g          137 t
ORIGIN      
        1 ttttaacttg aaacttaaaa attcttcaaa cactcttcta atgctattta ttgaatgata
       61 gtaataacaa tcttcctcaa ctatgtcctt ttgacttttt ctcgataact cgtaaatctc
      121 ttgaaataac tttttataag gcgtctttac tttcttaaat ttttctaaaa aactagttcc
      181 ctgatgatct ttgtacactt cataaaaatt atgatcttcc ccttttaatc tataagttat
      241 atcacaaaag tcattccaag aatgggtaaa tataaatatt tgattaaatt tgcttttaag
      301 tactttttta attaattcta aaacgtaaaa t
//
LOCUS       DATTQF010000327          322 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.denovo.349, whole genome shotgun sequence.
ACCESSION   DATTQF010000327 DATTQF010000000
VERSION     DATTQF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.denovo.349"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            135..>322
                     /locus_tag="VB241_002806"
     CDS             135..>322
                     /locus_tag="VB241_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1245999.1"
                     /translation="MYKNYNMTQLTLPIETSVRIPQNDISRYVNEIVETIPDSEFDEF
                     RHHRGATSYHPKMMLKII"
BASE COUNT          122 a           60 c           40 g          100 t
ORIGIN      
        1 ggcactgctc cctcaggagt ctcgccatta atactacgta ttaacatgta attttacttt
       61 gaaatacttt aaaaaaataa gacactttcg tataatttaa taaataccac taaactaaat
      121 taacgaggtg ccttatgtat aaaaattata acatgaccca acttacacta ccaatagaaa
      181 cttctgttag aattcctcaa aatgatattt cacgatatgt taatgaaatt gttgaaacga
      241 tacctgatag cgaattcgat gaattcagac atcatcgtgg cgcaacatcc tatcatccaa
      301 aaatgatgtt aaaaattatc tt
//
LOCUS       DATTQF010000328         5138 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.154, whole genome shotgun sequence.
ACCESSION   DATTQF010000328 DATTQF010000000
VERSION     DATTQF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.154"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            205..804
                     /locus_tag="VB241_002807"
     CDS             205..804
                     /locus_tag="VB241_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP1246000.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1173..1838
                     /locus_tag="VB241_002808"
     CDS             1173..1838
                     /locus_tag="VB241_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP1246001.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPVTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMNQNTHMN
                     QQPHMQQGHMQSSNHQMNQSNKKVLPAAGESMTSSILTASIAALLLVSGLFLAFRQRS
                     TNK"
     gene            complement(1940..2695)
                     /locus_tag="VB241_002809"
     CDS             complement(1940..2695)
                     /locus_tag="VB241_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP1246002.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2931..3638
                     /gene="deoD"
                     /locus_tag="VB241_002810"
     CDS             2931..3638
                     /gene="deoD"
                     /locus_tag="VB241_002810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP1246003.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIVAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            3645..4997
                     /gene="tet(38)"
                     /locus_tag="VB241_002811"
     CDS             3645..4997
                     /gene="tet(38)"
                     /locus_tag="VB241_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HEP1246004.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT         1771 a          776 c          892 g         1699 t
ORIGIN      
        1 aatggaagtt gtctttttta attctcttta aaagcgggaa acaaaagcag ttaaatgcct
       61 ttttgcattc aatattaaat attatatcaa tttcgaatat ttaaatttta tataattgga
      121 tataacaaat aaataataat tattgcaaaa cacacccaaa attaattatt ataaaagtat
      181 attcataaaa ggaggaatat acttatggca tttaaattac caaatttacc atatgcatat
      241 gatgcattgg aaccatatat agatcaaaga acaatggagt ttcatcacga caaacatcac
      301 aatacgtacg tgacgaaatt aaacgcaaca gttgaaggaa cagagttaga gcatcaatca
      361 ctagcagata tgattgctaa cttagacaag gtaccggaag cgatgaggat gtcagtccgt
      421 aataatggcg gtggtcattt taaccattca ttattctggg aaatactatc acctaattct
      481 gaagaaaaag gtggcgtaat agacgacatt aaagcgcagt ggggcacttt agatgaattt
      541 aaaaatgaat ttgcaaataa agcaacaaca ttatttggat caggttggac ttggttagtt
      601 gttaatgatg gaaaattaga aattgtgaca acgccaaacc aagataatcc attaacagaa
      661 ggcaaaacac caatcttact atttgatgtt tgggagcatg cctactatct gaaatatcaa
      721 aataaacgtc cagactatat gactgcattt tggaatattg ttaactggaa aaaagttgat
      781 gaattatacc aagcagcaaa ataatataac ttaatataat tgaggtggag catctattag
      841 gtgtttcacc ttttttgtac ttaatatctt tacttattcg tttttgtgta atgggatagc
      901 gcgtaaaagt ggcaaaataa taagataaat ttcaactcga aataccttat tattgttgac
      961 tataacgatg acttaaatgc tatatacatc agacaacact attgattaat caacatattg
     1021 acattaaata aattgacaaa ataagtaatt attgtgaatc taaagtgaaa tttttataaa
     1081 aaattgtaat gattcaaaaa ttttgttgca tttcttttgt aatcatatga taatataaat
     1141 gtaatcaaat tgtaatataa ggggaaaaga caatgaaaaa attagcaaca gtaggttctt
     1201 taattgtaac aagcacttta gtattctcaa gtatgccttt tcaaaatgcg catgctgaca
     1261 caacttcaat gaatgtgtcg aataaacaaa gccaaaatgt acaaaatcat cgcccttatg
     1321 gcggagtagt accacaagga atgacgcaag cacaatatac tgaattagag aaagctttac
     1381 cccaattaag cgctggcagt aatatgcaag actataatat gaaattgtat gatgcgacgc
     1441 aaaatattgc tgataaatac aatgtgataa ttacaactaa tgtaggggta tttaaaccac
     1501 atgctgttag agatatgaat ggccatgcgt tacctgtaac aaaagatggc aatttttatc
     1561 aaacgaatgt agatgcaaat ggtgttaatc atggtggtag tgaaatggtg caaaataaaa
     1621 caggtcatat gaatcagaac acacacatga accaacagcc acacatgcaa caaggtcata
     1681 tgcaatcatc aaaccatcaa atgaaccaaa gtaacaaaaa agttttacca gctgctggtg
     1741 aaagtatgac atcaagtatt cttactgcaa gtattgccgc actactatta gtatctgggt
     1801 tattcttagc atttagacaa cgttcaacaa ataaataaac atcatacgtt ttaaagtaga
     1861 aaaatcatgt gattacctga aagttattgt cgattaaaca atagctaaac agtcatcaca
     1921 tgattttttt tcaataattt taataatccg attgtcttat acgtgtcagt gttaattcag
     1981 atatttcctg tggaatatac cacttattaa tcataatcgg atagggcgtt tgtgcgtaca
     2041 atgtttcaat aatcagccaa caatgtgtat caccatcaaa cacgtgacta tgatttttga
     2101 agtggggcgc tttggtaata gacattttta aatctgattg atatgcattg ctataaatcg
     2161 tttgctcaac gaacgtcttc atgtcgtctt cgttttgtgt attcacttta aatgtgtcaa
     2221 tgacatttaa cggtataaag gtaaaacaaa atgcatcagc ttgcttagaa tggttgtcct
     2281 ttttttgata atagcgttcc attgcaatga cggcagaagg atggtttgca aacaaatgat
     2341 ttgtatattc actttctaaa tcaacacgat aattaattga tgacatagat acacgatcta
     2401 ataatatttg atcaagtgga tgcttaaatt gatccatact tgaagcgtgt tgggcatttg
     2461 tttgtggaat aacaaagtgt cccttccctc ttgtactctc tacgatgcca tcttcggcta
     2521 acaattttat agcttggcgc aaagtcatac gactgacatc aaagcgcgca caaagttcct
     2581 tttcagtagg taatgcatgg ccactcggat attttcctat ttgaatttct ttatataacg
     2641 tattataaat cgttaaaaat tttggttgtg tttgcgtcac gtagacaacc tccataaagt
     2701 tacttaatca ttctcatcat acaataattt ttactcgaat tggaaaaatt ataaaaatta
     2761 aacatagaaa caacttgaac attggtttta tgcaagatca gtagagataa ctgtaaaatg
     2821 ttcttaatat tgtcaaaatg taatgcttga aagcgctttt aaaaaatatt attatataca
     2881 tggttagaca aatagacaaa ttactataca aatattggga ggaatatttt atgaaatcaa
     2941 caccacacat taaaccaatg aatgacgtcg aaattgcaga aacggttcta ttgccaggag
     3001 atccgttaag agctaagttc attgcagaaa catatttgga cgatgtggaa cagttcaata
     3061 cagtgcgaaa tatgtttggt tttaccggaa catataaagg taaaaaagtt tctgtcatgg
     3121 gttcaggaat gggtatgcca tctattggca tttactctta tgaattaatt catacatttg
     3181 gttgtaaaaa attaattcgc gttggttctt gtggcgcgat gcaagaaaac attgatttat
     3241 atgatgtgat tgttgcacaa ggtgcctcta ctgattcaaa ttacgttcaa caatatcaat
     3301 taccaggtca ttttgcgcca attgcttctt atcagttatt agaaaaagct gttgaaacag
     3361 cacgtgacaa aggtgtacgt catcatgtag gtaatgtgtt atcaagtgat attttctata
     3421 acgcggatac aacagcgagt gaacgttgga tgcgtatggg tattttaggt gtagaaatgg
     3481 aatcagctgc attatacatg aatgcaattt acgctggtgt cgaagcatta ggtgtgttca
     3541 cagtgagcga tcatttaatt catgaaacgt caacaacacc tgaggaaagg gaacgtgcat
     3601 ttacagatat gattgaaatt gcactgtcat tggtgtagat gattatgaat gttgaatatt
     3661 ctaaaataaa gaaagcagta cctattttat tattcttatt tgtattcagt ttggttatag
     3721 acaactcatt caaattgatt tctgtagcca ttgctgatga cttaaacatt tcagtaacga
     3781 cagtaagttg gcaagcgaca ttagccggtt tagtaattgg tattggcgct gtagtatacg
     3841 cttcattatc tgatgccatt agtatacgca cactatttat ttatggcgtg atattaatca
     3901 ttatcggatc aattattggt tacattttcc aacatcaatt cgcattactt ttagttggac
     3961 gtattattca aactgcaggt ttagctgctg cagagacatt atatgtgata tatgttgcaa
     4021 agtatctttc taaagaggac cagaagactt accttggctt aagtacgagc agttattcct
     4081 tgtcattagt tatcggtaca ttatcaggtg gatttatttc tacgtattta cactggacaa
     4141 atatgttttt aattgcatta atcgtagtat ttacgttgcc attcctattt aaattattac
     4201 caaaagaaaa taatacaaat aaagctcatt tagattttgt tggcttaatt ctagtggcaa
     4261 ctatcgctac aacagttatg ctgtttatta cgaactttaa ttggttatat atgattggtg
     4321 ccttaattgc gattatcgtt tttgcgctat atattaaaaa tgcgcaacgt ccattagtaa
     4381 acaaatcatt tttccaaaat aaacgttatg cttcattttt atttatagta tttgtaatgt
     4441 atgccatcca attgggttat atttttacgt tcccattcat aatggaacaa atttatcatc
     4501 ttcagctaga cacaacatca ctgctattag taccgggtta tattgtagca gtcatcgttg
     4561 gtgcactaag tggtaaaatc ggcgaatatc ttaattcaaa acaagcgatt atcacagcaa
     4621 ttattttaat cgcactgagc ttgattttac ctgcatttgc agtaggtaat cacatttcaa
     4681 tcttcgtcat ttctatgata ttctttgcag gtagctttgc tttaatgtat gcacctttac
     4741 ttaacgaagc cattaaaaca atagatctta atatgacagg tgtggctatt ggtttttata
     4801 atttaattat taatgtggcg gtatctgtag gtattgcgat tgctgcggct ttaatcgatt
     4861 ttaaagcatt aaatttccca ggcaatgatg cattaagttc acatttcggt attattttaa
     4921 ttattttagg tttaatgagt attgtcggat tagttttatt cgtcatctta aatcgttgga
     4981 cacaatctga aaaataaata gatataaatt cgcgagatat attcgtattt atagtaaaat
     5041 taaataaaga gattatataa cacgaggagt agtaagtatg aaatttgaga aatatataga
     5101 tcacacttta ttgaagcctg agtcaacacg tacgcaaa
//
LOCUS       DATTQF010000329         4807 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.166, whole genome shotgun sequence.
ACCESSION   DATTQF010000329 DATTQF010000000
VERSION     DATTQF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.166"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            89..283
                     /locus_tag="VB241_002812"
     CDS             89..283
                     /locus_tag="VB241_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEP1246005.1"
                     /translation="MSRILTYSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            295..546
                     /locus_tag="VB241_002813"
     CDS             295..546
                     /locus_tag="VB241_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HEP1246006.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            584..1708
                     /locus_tag="VB241_002814"
     CDS             584..1708
                     /locus_tag="VB241_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HEP1246007.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEDILPDIKAAFSGESQVLESGKDPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTKHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMTNRTI"
     gene            1724..2161
                     /locus_tag="VB241_002815"
     CDS             1724..2161
                     /locus_tag="VB241_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEP1246008.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEEVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGRDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            2585..3541
                     /locus_tag="VB241_002816"
     CDS             2585..3541
                     /locus_tag="VB241_002816"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP1246009.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            3741..4220
                     /locus_tag="VB241_002817"
     CDS             3741..4220
                     /locus_tag="VB241_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP1246010.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            4235..>4807
                     /gene="fakB2"
                     /locus_tag="VB241_002818"
     CDS             4235..>4807
                     /gene="fakB2"
                     /locus_tag="VB241_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HEP1246011.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTFNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLD"
BASE COUNT         1778 a          691 c          867 g         1471 t
ORIGIN      
        1 gggtcaaaaa tgttataata tttttatgtc aaatttaaaa cagtaacact tatttacaag
       61 gttgcaatat tttgaagtaa taaaggaagt gtcgcgtatt ttaacttatt cagagcaaaa
      121 tgcactcgcg aaaatagatg atttaatgaa tacttattgc aatcaatgtc caatcaaaac
      181 tcgtctgcgt aaattagagg ggaaaacgaa ggcgcatcat ttttgtatca atgagtgttc
      241 aatagggaaa gaaataaaac aattaggaaa tgaacttcaa taggaggaag tcaaatgcaa
      301 attatatcta tatcagaaac accgaaccac aacacaatga agattacact tagtgaaagc
      361 agagaaggta tgacatcaga tacgtatact aaagttgatg attcacagcc agcatttatt
      421 aatgacatct taaaggttga aggcgttaaa tcaattttcc atgttatgga ctttatttca
      481 gtagataaag aaaatgacgc aaattgggaa acagtattgc caaaagtaga ggctgtattc
      541 gaataaattt ttcatcaact agtattcggg gggaataaag tatatggaaa ttttacgtat
      601 agagccaaca cctagtccaa atacaatgaa agttgttttg tcatatacaa gagaagacaa
      661 gttatctaat acttataaaa aagtagaaga aaatcaacca agatttataa atcagttgtt
      721 atctatagat ggtatcactt ccatttttca tgtcatgaac ttcttagctg ttgataaggc
      781 accaaaagct gattgggaag acatactacc tgatattaaa gctgcttttt caggtgaaag
      841 tcaggtgttg gaatctggaa aagacccaca aattgacaat cattttggtg aaattaaagc
      901 tgaactatta acttttaagg gtataccgta tcaaattaag ctaacttctg ctgaccaaga
      961 attaagagaa caattaccac aaacatatgt tgatcatatg actcaagcgc aaacaaaaca
     1021 tgacaatatt gtttttatgc gtaaatggct agatttagga aatcgctatg gaaatattga
     1081 agaagtaatg gatggtgtcc tagaagaagt actagctacc tatccagaat cacagttacc
     1141 tgtattggta aaacatgctt tagaagaaaa tcacgcaact aataattatc atttctatcg
     1201 acatgtctct ttggatgaat atcatgcgac tgataattgg aagactcgat taagaatgtt
     1261 aaatcatttt ccaaagccga cttttgaaga tataccgctg cttgatttag ctttatctga
     1321 tgaaaaagta ccggttagac gtcaagcgat tgtactatta ggtatgattg aaagtaaaga
     1381 aattttaccg tatttatata aggggcttcg tgataaaagt cctgctgtaa gaagaactgc
     1441 aggggattgc ataagcgatt tagggtatcc agaggcacta ccagaaatgg tgctactatt
     1501 agatgatcca cagaaaatcg ttaggtggcg tgctgctatg tttatctttg atgaaggtaa
     1561 tgcagagcag cttcccgcac ttaaagccca tattaatgac aatgcgtttg aagttaaatt
     1621 acaaattgaa atggccatat ctcgtattga aaacggagat gaagcacttg gttcagtatg
     1681 gaaacaaatg acgaacagaa caatttaatt ggaggaatta aatatgaatg catatgatgc
     1741 ttatatgaaa gaaattgcgc aacaaatgcg tggcgaatta actcaaaatg gttttacaag
     1801 tttagaaacg agcgaggaag tatcagagta tatgaaccaa gtaaatgctg atgacactac
     1861 ttttgtagtt attaactcta catgcggctg tgcagctgga ttagcaagac cagctgcagt
     1921 agctgttgca acacaaaatg aacatagacc tacaaataca gttacagttt ttgctgggca
     1981 agataaagaa gcaactgcta caatgcgaga attcattcag caggtaccat ctagtccttc
     2041 gtatgcttta ttcaaaggtc gagatttagt ttattttatg cctagagaat ttatcgaagg
     2101 tagagatatt aatgacattg caatggactt aaaggatgcc tttgacgaaa attgtaaata
     2161 gtacacataa ataaatataa aggttaacac attttataat attaaaaatg gtgtctgtca
     2221 ttgaaaatag agaatatagt tgtattccat ttgttatata aagaacgttc ttaccaacta
     2281 tattttctag aaatttaact gttttaatag gacatcaaac ataatattca aatcatgtgt
     2341 taacctcttt tttaaaattt tttagcatta aagttataga tttgggtaaa caattagcaa
     2401 ttggaaacat agatcacgtt atgatgtggc aggtacttaa tcatcatttt ataaataaag
     2461 taacggaatt catgatatta atatcatatt cctaaaatga gtgataacaa aatgctacat
     2521 aaagttaaat tatatcgaac taaatataca tactataaat aataaaaatg aggtgttatc
     2581 gcatatgttg aattcatttg atgcagcata tcacagtctt tgtgaagaag ttttagaaat
     2641 aggaaataca cgaaatgatc gcacaaatac aggtacgatt tcgaaatttg gtcatcaact
     2701 tcgctttgac ttatctaaag gatttccact attaacgaca aagaaagttt cttttaaatt
     2761 agtagcgacc gaattattat ggttcattaa aggagataca aatatccaat acttattaaa
     2821 gtataataat aatatttgga acgaatgggc ttttgaaaat tatatcaaat cagacgagta
     2881 taaaggtcca gatatgacag atttcgggca tcgtgcattg agtgatcctg aatttaacga
     2941 acaatataaa gaacaaatga aacaatttaa gcaacgtatt cttgaagatg atacatttgc
     3001 gaagcaattc ggtgatttag gaaatgttta tggtaaacag tggcgagatt gggttgataa
     3061 agatggtaat cattttgatc aacttaaaac agtaattgag caaattaagc ataatccaga
     3121 ttcaagacga cacatcgtat ctgcatggaa tccaacagaa attgatacaa tggcacttcc
     3181 gccttgtcat acaatgttcc agttttatgt tcaagatggt aagttaagtt gccagttata
     3241 ccaacgtagc gcagatatct ttttaggtgt gccatttaat atcgcaagct acgctttatt
     3301 gacgcacctt attgccaaag aatgtggact tgaagtagga gaatttgtgc atacatttgg
     3361 agatgcacat atttattcaa atcatattga tgcgattcaa acacaattag cacgtgaaag
     3421 cttcaatcct ccaacattaa aaattaacag tgataagtct atttttgaca taaattatga
     3481 agatttggaa attgttgact atgaatcaca tccagcaata aaagctccaa tagcagtgta
     3541 gtcgttgcat agttagctaa ccatatagac atcaaaatga catcaaagta ttttcaagtg
     3601 caaaaaagta cttttttgtg ttaaacgttt tcataaatta tgcaaaatca ttatttctat
     3661 cacactttat gataaaaatt gtgttaaatt aaagataact tagtaattaa aaatgaaatg
     3721 atagaagaag gaggataatc atgactttat ccattctagt tgcacatgac ttgcaacgag
     3781 taattggttt tgaaaatcaa ttaccttggc acctaccaaa tgatttgaag catgttaaaa
     3841 aattatcaac aggtcatact ttagtaatgg gtcgtaagac atttgaatcg attggtaaac
     3901 cactaccgaa tcgtcgaaat gttgtactta cttcagatac aagtttcaac gtagagggcg
     3961 ttgatgtaat tcattctatt gaagatattt accaactacc gggccatgtt tttatatttg
     4021 gagggcaaac attatttgaa gaaatgattg ataaagtgga cgacatgtat attactgtta
     4081 ttgaaggtaa atttcgtggt gatacgttct ttccacctta tacatttgaa gactgggaag
     4141 ttgcctcttc agttgaaggt aaactagatg agaaaaatac aattccacat acctttctac
     4201 atttaattcg taaaaaataa gggggaaaac gaccatgaca aaacagatta tagtaacaga
     4261 ctcaacatcc gatttatcta aagaatattt agaagcaaac aacatacatg taattccttt
     4321 aagtttaact attgaaggag cttcatacgt tgaccaagta gatattacat cagaagaatt
     4381 tattaatcat attgaaaatg atgaagatgt aaagacaagt cagccagcca taggtgaatt
     4441 tatatctgct tatgaagaac taggaaaaga tggttctgaa atcataagta ttcatctttc
     4501 ttcaggacta agtggtacat ttaacactgc ttaccaagca agtcaaatgg tagatgctaa
     4561 tgtaactgtt attgattcaa aatctatttc ttttggttta gggtatcaaa tacaacacct
     4621 agtagagctt gtaaaagaag gtgtctcaac ttctgaaata gttaaaaagt taaatcattt
     4681 aagagaaaac attaaattat ttgtagttat agggcaattg aatcaattaa ttaaaggtgg
     4741 cagaattagt aaaacaaaag gtttgattgg taatcttatg aaaattaaac caattggtac
     4801 actagat
//
LOCUS       DATTQF010000330         3868 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.183, whole genome shotgun sequence.
ACCESSION   DATTQF010000330 DATTQF010000000
VERSION     DATTQF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.183"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            116..535
                     /gene="fosB"
                     /locus_tag="VB241_002819"
     CDS             116..535
                     /gene="fosB"
                     /locus_tag="VB241_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HEP1246012.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFKLAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(791..1726)
                     /locus_tag="VB241_002820"
     CDS             complement(791..1726)
                     /locus_tag="VB241_002820"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEP1246013.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSTPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFATLAAKSIVNHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTGYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFIHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            2004..3263
                     /gene="sdpC"
                     /locus_tag="VB241_002821"
     CDS             2004..3263
                     /gene="sdpC"
                     /locus_tag="VB241_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HEP1246014.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSVIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKIQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYDTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTES
                     QHHIDNQTESNHDEDHDITSKSVESAESVKHAPQSDDLTNDSNEDETEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(3329..>3868)
                     /locus_tag="VB241_002822"
     CDS             complement(3329..>3868)
                     /locus_tag="VB241_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEP1246015.1"
                     /translation="NITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPS
                     LNIIKGGGGALFREKVIDEMASRFVVVVDETKMVQYLGETFKLPVEVDKFNWYHILRK
                     IESYADIKVERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFL
                     DMADQVIVGTQEGVKILEK"
BASE COUNT         1332 a          716 c          540 g         1280 t
ORIGIN      
        1 ttattagagc ctcttatgca gttgctcagt caactgtata aaaattaatg acgtcatttc
       61 aaaaatcgat acaaaaataa tttattataa aaattctaag aaagaagtga acccgatgtt
      121 aaaatctatt aatcatatat gcttttcagt cagaaattta aacgattcaa tacattttta
      181 tagagatatt ttacttggaa aattgctatt gactggtaaa aaaactgctt attttaagct
      241 tgcaggccta tggattgctt taaatgaaga aaaagatata ccacgtaatg aaatccactt
      301 ttcatataca catatagctt tcactataga tgacagcgaa tttaaatatt ggcatcagag
      361 gttaaaagat aataacgtga atatcttaga aggaagagtt agagatatta gagatagaca
      421 atcaatttac tttactgacc ctgatggtca taagctagaa ttacatactg gcacacttga
      481 gaacagatta aattattata aagaggctaa accacatatg acattttaca aataaggcgt
      541 cattataaaa aggcctcttg aactccgtta aaattttaat taattattat ataataagag
      601 aacttttcaa acaatacagt tgttattttt actatctcaa caaacataaa taagcagtaa
      661 gatgactaca acttaagagt cttcttactg caattatctt tcaaatatat caacgttaat
      721 ataacttcta ttacgaaata ctcacattct gccctgcaat gcaaatttcg tcacatataa
      781 atatttttaa ttattttaaa aaatgatgca ctaaattagc aacgagcttc gcagttctat
      841 tgtcagcgtc atatgttgga ttcatctcag caatactaac tgaagacacc ttatcacttg
      901 gaataatacg ttttgccaat tcaagaacag tatgtggata caaacctaac actgccggcg
      961 cacttacccc aggcgcaaat gcactatcaa tgacatccat acaaatcgta aacataatga
     1021 catcatgttc atgtataaaa cgctcaatca tatctttgat tgttggtgat acgtgactca
     1081 ataattcatc tgcaaagaca taatcaatct ttttctcttt agcataatca aataaacttt
     1141 gcgtattccc accttgagca ataccaagca ctaaataacc tgtgttttca tcttcttcta
     1201 aaatttgtct aaagctcgtt ccagatgtag attgttgttc agcacgtgta tcaaaatgcg
     1261 catcaatatt tatcacacca atagattgtg ttggatagac tttacgcgtt gctaaatatt
     1321 gagcatacgc aatatcatgt ccaccaccta ataaaaacgt ttgtctatga ttaacaattg
     1381 acttcgctgc aagtgttgca aattcttttt gagtatcaat taattcctca tgatcatgat
     1441 aaacatttcc gtaatcgact aaagtttcac attgattcaa atccggcaaa cctgcaaatg
     1501 cttgtttaat cgcatctggt ccttcttttg caccaatgcg ccccttgttt aaagcaacac
     1561 ctttgtcaac agcatagcct aatataccaa cccctgatgg tgtactactt ttttccaatt
     1621 tcgacaaatc ttcaaatgtc actgtttgaa aatgcctgaa ttttttcggg tctgtttcac
     1681 tatctaacct tccagtccat aaatttggtt caccttgctt gtacacagca tttccccctc
     1741 ttatttatgt ggctttttaa caattaaagt ataacgtact acaaattttg aattcaattc
     1801 ataattaaaa agtacttatc tatatacctt ttacccctat tttctattca ctaacgaaca
     1861 cgaaagcgat ttatgttaat aaaatacgtc aagactatta cattttcatt aatattagca
     1921 cagacaattt aactcacggc ttgtaatatg tataattgtt acaaaatgat attttgcttg
     1981 ttacctaatg gaggttacat ataatgaaaa acaataaaat ttctggtttt caatgggcaa
     2041 tgacgatctt cgtctttttc gttattacaa tggcgttatc cattatgctt agagatttcc
     2101 agtctgtcat tggtgtcaaa cactttatat ttgaagttac agatctagca ccattaattg
     2161 ctgcaatcat ttgtatactc gttttcaaat ataaaaagat ccaacttgca ggtttaaaat
     2221 tctcaatcag cctgaaagta attgaacgtc tactgctagc tttaatttta cctttaatta
     2281 ttctaattat tggtatgtac agctttaata catttgcaga tagctttatt ttattacaat
     2341 caacaggctt atcagtacct attacacata ttctgattgg acatattcta atggcgttcg
     2401 tagtagaatt cggattccgt tcatacttac aaaatattgt tgaaacgaaa atgaatacgt
     2461 tctttgcaag catagttgtt ggtttaatgt actctgtatt ttcagcaaat acaacttacg
     2521 atacagaatt tgcagcttat aatttccttt acacattctc attctcaatg attctaggtg
     2581 agttaattag agctacaaaa ggacgtacga tctacatcgc aacaacattt catgcttcaa
     2641 tgacatttgg ccttattttc ttatttagcg aagaaatcgg tgatttattt tcaattaagg
     2701 tcattgctat ttcaacagca attgtagcgg ttggctatat tggtttaagc ttaattattc
     2761 gaggtattgc ttatttaaca actagacgaa accttgaaga acttgagcct aataattatt
     2821 tagaccatgt caatgacgat gaagaaacta atcatactga ggctgaaaaa tcttcttcaa
     2881 atattaaaga tgctgaaaaa acaggtgtag ctactgcatc aacggtcggt attgctaaaa
     2941 atgatactga aaatactgtg gctgacgaac caagcattca tgaaggtact gaaaaaacag
     3001 aatctcaaca tcacatagat aatcaaactg aatctaatca tgatgaagat catgacatca
     3061 cttcgaagtc agtagaatca gcagaatcag ttaaacatgc accacaaagt gacgatttaa
     3121 caaacgattc aaatgaagat gaaacagagc aatcattaaa agaacctgcg acctataaag
     3181 aagacagacg ttcatcagtt gtaatcgatg cagaaaaaca tatcgaaaaa actgaagaac
     3241 aatcttcaga taaaaacaaa taataacaaa gcgcttgcta gtacctctta aaaagatgat
     3301 gctagcaagc gcttttctat actatatatt atttttctaa aattttaacg ccctcttgag
     3361 tacctacaat aacttgatct gccatatcta agaagtagcc tgtctcaaac acacctgtca
     3421 gatgaattaa atactcatgg aacttatatg ggtcaattcc ttttggtaac ttacaatcta
     3481 aaatataatt gccattatca gttataaatg cgacatcttc gtttacacgg cgttctactt
     3541 ttatatcagc gtatgattca attttacgta atatgtgata ccagttaaat ttatccacct
     3601 ctactggtaa cttaaacgtc tcacctaagt attgaaccat tttcgtttca tcgacaacca
     3661 caacaaaacg tgatgccatt tcatctatca ctttctctct gaacagcgca ccaccaccac
     3721 ctttaattat atttaaagat ggatctactt catcagcacc atcaattgct aagtcgatat
     3781 gatcaacatc attgatttcg catattttaa tacctagttc ttttgctaaa aatgcaattt
     3841 tattagaagt gcatacacct gtaatatt
//
LOCUS       DATTQF010000331         3257 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.196, whole genome shotgun sequence.
ACCESSION   DATTQF010000331 DATTQF010000000
VERSION     DATTQF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.196"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            <1..68
                     /locus_tag="VB241_002823"
     CDS             <1..68
                     /locus_tag="VB241_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP1246016.1"
                     /translation="KENKKLQNQIQKLYGDLYNKE"
     gene            219..1001
                     /locus_tag="VB241_002824"
     CDS             219..1001
                     /locus_tag="VB241_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEP1246017.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1127..1858)
                     /gene="erm(A)"
                     /locus_tag="VB241_002825"
     CDS             complement(1127..1858)
                     /gene="erm(A)"
                     /locus_tag="VB241_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEP1246018.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1916..1975)
                     /locus_tag="VB241_002826"
     CDS             complement(1916..1975)
                     /locus_tag="VB241_002826"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEP1246019.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2367..3029
                     /locus_tag="VB241_002827"
     CDS             2367..3029
                     /locus_tag="VB241_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP1246020.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1101 a          465 c          590 g         1101 t
ORIGIN      
        1 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
       61 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
      121 gctttattga acagtaattt aagtatatgt ccaatctagg gtaagtaaat tgagtatcaa
      181 tataaacttt atatgaacat aatcaacgag gtgaaatcat gagcaatttg attaacggaa
      241 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
      301 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
      361 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
      421 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
      481 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
      541 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
      601 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
      661 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
      721 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
      781 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
      841 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
      901 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
      961 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
     1021 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
     1081 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
     1141 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
     1201 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
     1261 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
     1321 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
     1381 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
     1441 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
     1501 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
     1561 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
     1621 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
     1681 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
     1741 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
     1801 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
     1861 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
     1921 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
     1981 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
     2041 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
     2101 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
     2161 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
     2221 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
     2281 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
     2341 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
     2401 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
     2461 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
     2521 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
     2581 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
     2641 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
     2701 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
     2761 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
     2821 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
     2881 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
     2941 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
     3001 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
     3061 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
     3121 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
     3181 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
     3241 taatttactc ttgataa
//
LOCUS       DATTQF010000332         2079 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.228, whole genome shotgun sequence.
ACCESSION   DATTQF010000332 DATTQF010000000
VERSION     DATTQF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.228"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            1..1424
                     /locus_tag="VB241_002828"
                     /pseudo
     CDS             1..1424
                     /locus_tag="VB241_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
     gene            1414..1794
                     /gene="blaI"
                     /locus_tag="VB241_002829"
     CDS             1414..1794
                     /gene="blaI"
                     /locus_tag="VB241_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HEP1246021.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          862 a          229 c          307 g          681 t
ORIGIN      
        1 gttttagtta ttattttaag ttttaaattt ttgaaagcct tattatatct taaatattta
       61 aagaaacagt cactttatct aaacgaaaat gaaaaaaata aaatagatac gatacttttc
      121 aaccatcaat ataaaaaaat attgtgattc gaaaagcaga gactattcaa tctccaataa
      181 ctttttggta tgggaaatat attattttga ttcctagttc atattttaaa agtgtaattg
      241 acaaaagact aaaatatata atattgcatg aatatgctca tgctaaaaat agagatactt
      301 tacatttaat tatctttaat atcttctcta ttattatgag ttacaatcca ttagtacata
      361 ttgtaaaaag aaagataatt catgacaatg aagtagaagc cgatagattt gtccttaata
      421 atattaacaa aaatgaattt aaaacttatg cggaatctat tatggactcc gtattaaatg
      481 ttccattttt taataaaaat atattaagcc attcatttaa tggtaaaaag tcattactca
      541 aaagaagatt aattaatata aaagaagcca atctgaaaaa acagtccaaa ttaattctaa
      601 tttttatttg tatttttact tttttgctca tggtaattca aagtcagttt ttgatggggc
      661 aatccataac tgattataat tataaaaagc ccttacacaa cgattaccaa attttagata
      721 aaagtaaaat ttttggttca aatagtggat ctttcgtcat gtacagcatg aagaaagaca
      781 aatactatat ttataacgaa aaagaaagta gaaaaaggta ttctcctaat tcaacttata
      841 aaatttattt agctatgttt ggacttgacc gacatattat aaatgatgaa aattcacgta
      901 tgtcatggaa tcataagcat tacccttttg atgcttggaa taaggaacaa gatttaaata
      961 cagcaatgca aaattcagtt aattggtact tcgaacgtat tagcgatcaa ataccaaaga
     1021 actatactgc gactcaactc aagcaattaa attatggtaa taaaaatttg ggaagttata
     1081 aaagctattg gatggaagat agtttgaaaa tatctaatct tgaacaagta atagttttta
     1141 aaaatatgat ggaacaaaat aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt
     1201 cattattgat taagaaaaat gaaaagtatg aactgtatgg gaaaacaggt acaggtatag
     1261 taaacgggaa gtataataat gggtggtttg taggttacgt aattacaaat catgataagt
     1321 attattttgc tacacattta tcagatggaa agccatctgg gaaaaatgct gaattaatca
     1381 gtgaaaaaat attaaaagaa atgggtgttt taaatggcca ataagcaagt tgaaatatct
     1441 atggctgaat gggatgttat gaatataata tggggtaaaa aatcagtatc agctaatgaa
     1501 attgtagttg aaattcaaaa atataaagaa gttagcgata aaacgattag aacattaatc
     1561 acaagactat ataaaaaaga gattataaaa cgatacaaat cagagaatat ttatttttac
     1621 tcatcaaata ttaaagaaga cgatattaaa atgaaaactg ctaaaacctt tcttaataaa
     1681 ctgtatggag gggacatgaa aagtttagtg ctgaattttg cgaaaaatga agaattaaat
     1741 aacaaagaaa ttgaagaatt gcgagacatt ttaaatgata ttagtaaaaa gtaattagag
     1801 atatgtttca aacataatac atatgtaaac tatgcttgac tcttattaaa ttagttttgt
     1861 taataacatt taaaatatat tattaaagat gagagtgtac agaaaataac cattttctgt
     1921 acactctctt tttatgccga aataagtgag taatacatat attttcgtgt tcaaaaaccc
     1981 aacaaaaaat ctatgttcac aaaagacctt gaaattaagt tatggtacac tgtatatgta
     2041 tataggaggt ttgtattttg aaaataggtt atgcaagag
//
LOCUS       DATTQF010000333         1034 bp    DNA     linear   BCT 27-DEC-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain H30 isolate 30
            SAMN07257427-rid20656623.guided.264, whole genome shotgun sequence.
ACCESSION   DATTQF010000333 DATTQF010000000
VERSION     DATTQF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07257427
            Sequence Read Archive: SRR5714697
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002040057.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002040057.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/27/2023 18:42:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,830
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,682
            CDSs (with protein)               :: 2,682
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 50 of 81
            Pseudo Genes (incomplete)         :: 18 of 81
            Pseudo Genes (internal stop)      :: 28 of 81
            Pseudo Genes (multiple problems)  :: 13 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07257427-rid20656623.guided.264"
                     /strain="H30"
                     /isolate="30"
                     /isolation_source="Human colonisation"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand"
                     /collection_date="2014"
                     /collected_by="Auckland Hospital"
     gene            169..1014
                     /locus_tag="VB241_002830"
     CDS             169..1014
                     /locus_tag="VB241_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HEP1246022.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          437 a          124 c          150 g          323 t
ORIGIN      
        1 aataataaca atagaaatat aaaacaaaag cttactatgc tcattattaa taacttagcc
       61 atttcaacac cttctttcaa atatttataa taaactattg acaccgatat tacaattgta
      121 atattattga tttataaaaa ttacaactgt aatatcggag ggtttatttt gaaaaagtta
      181 atatttttaa ttgtaattgc tttagtttta agtgcatgta attcaaacag ttcacatgcc
      241 aaagagttaa atgatttaga aaaaaaatat aatgctcata ttggtgttta tgctttagat
      301 actaaaagtg gtaaggaagt aaaatttaat tcagataaga gatttgccta tgcttcaact
      361 tcaaaagcga taaatagtgc tattttgtta gaacaagtac cttataataa gttaaataaa
      421 aaagtacata ttaacaaaga tgatatagtt gcttattctc ctattttaga aaaatatgta
      481 ggaaaagata tcactttaaa agcacttatt gaggcttcaa tgacatatag tgataataca
      541 gcaaacaata aaattataaa agaaatcggt ggaatcaaaa aagttaaaca acgtctaaaa
      601 gaactaggag ataaagtaac aaatccagtt agatatgaga tagaattaaa ttactattca
      661 ccaaagagca aaaaagatac ttcaacacct gctgctttcg gtaagacttt aaataaactt
      721 atcgcaaatg gaaaattaag caaagaaaac aaaaaattct tacttgattt aatgttaaat
      781 aataaaagcg gagatacttt aattaaagac ggtgttccaa aagactataa ggttgctgat
      841 aaaagtggtc aagcaataac atatgcttct agaaatgatg ttgcttttgt ttatcctaag
      901 ggccaatctg aacctattgt tttagtcatt tttacgaata aagacaataa aagtgataag
      961 ccaaatgata agttgataag tgaaaccgcc aagagtgtaa tgaaggaatt ttaatattct
     1021 aaatgcataa taaa
//
